LOCUS       DASUJG010000001         2328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_856213, whole genome shotgun sequence.
ACCESSION   DASUJG010000001 DASUJG010000000
VERSION     DASUJG010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856213"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..1609)
                     /locus_tag="VFX38_00005"
     CDS             complement(152..1609)
                     /locus_tag="VFX38_00005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEX5313283.1"
                     /translation="MSTEIVVNAAPYETRVAVVEGGAVQEIYLERPGRRSLVGNVYRG
                     RVMRVLPGMQAAFVDIGDRRSALLQDGTGGEARRAENGPSLSLAAVLREGEALLVQVR
                     KDPLGSKGARLTTRIRLPSRYLVREPGGEGVAVSARIADGEERERLRRLVTELARGAS
                     PGAWLLRTAAAGVSAAELSGEMHRLVRLSDSLVRAATTVSPPALVHEEPRLALRLLRD
                     LPGGELTAMRVDAESLWGAARDFAAEFAPDCTDRIHYHDGAQPIFDQYGIEEEITGAL
                     GRRVKLRSGACLVFDETEALTSIDVNSGARVRGRDFASTALAVNLEAAEAIARQLRLR
                     NLGGIIVIDFIDMAKVEHRAMVLETFRRALARDRARSVVGEISQFGLVEMTRERNRES
                     LAGARSESCTICRGGGRRPTNESVCFEIYRELERLARNVAASGYLVLAATRLVELLRG
                     EEASNLAAVCARIGRSVTLQSESVYRPEQFDVVAL"
     gene            complement(1606..2172)
                     /locus_tag="VFX38_00010"
     CDS             complement(1606..2172)
                     /locus_tag="VFX38_00010"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX5313284.1"
                     /translation="MPAILTLASSSPRRRALLARIGLRFRVEAPRISEAIAAGEDAET
                     LIRRLAGAKAAEVAERIGACALGADTVVVCGAAIFGKPADDEAAEEMLAQLSGREHRV
                     VTAVALAGEGPTAVATSLTRIWFRPLDAATRGRYVASGEGRDAAGAYRIQGAASAFLV
                     RLAGSYSNVVGLPLAETVQLLRNAGLVP"
     gene            complement(2233..>2328)
                     /locus_tag="VFX38_00015"
     CDS             complement(2233..>2328)
                     /locus_tag="VFX38_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEX5313285.1"
                     /translation="LTLPHALVRVIVAEQLYRAISLNAGHPYHRS"
BASE COUNT          413 a          824 c          734 g          357 t
ORIGIN      
        1 cgcgctcacc tcggactgaa tcttgtcgtg ataacgcggg agcatggcgg ccgcgagaaa
       61 gaagagcccc accgcaatcg cgacgatggc gagcaccgcc acgacgatcc cgatcgtcca
      121 gtggcgcagc cggtgtgtcg cgggccgcga ttcagagggc gacgacatcg aactgctccg
      181 ggcgatagac cgattccgac tgcaatgtca ccgagcgtcc gatgcgcgcg cagaccgccg
      241 cgagattgga agcctcctcc ccgcggagaa gttcgaccag ccgggttgcg gcaagcacga
      301 gatagccgct cgccgcgaca ttcctcgcga ggcgttcgag ttcgcgatag atttcgaagc
      361 acacgctttc gttcgtcggc ctgcgcccgc ctccgcgaca gatcgtgcag gattcgcttc
      421 gcgcgccggc gagactttcg cggttgcgtt cgcgggtcat ttctacgaga ccgaactgcg
      481 aaatctcgcc gacgacgctc ctcgcccgat cgcgggcgag tgcacgccga aacgtttcca
      541 gcaccattgc ccggtgttcg actttcgcca tgtcgatgaa gtcgatgacg atgatgcctc
      601 ccagattgcg cagccgcaat tgccgcgcga tcgcctcggc ggcttcgaga ttgaccgcga
      661 gcgcagtgga tgcgaaatcg cgaccgcgca cccgagcgcc gctgttcacg tcgatactcg
      721 tgagcgcctc ggtttcatcg aagaccagac aggcgccgga tcgcagcttc acccgccgcc
      781 cgagcgcgcc cgtgatctcc tcctcgattc cgtactggtc gaagatcggt tgcgcgccgt
      841 cgtggtaatg aatgcggtcc gtgcaatccg gagcaaactc ggccgcgaaa tctcgcgccg
      901 cgccccacag cgattcggca tcgacccgca tcgccgtcag ctcgccgccc ggcagatctc
      961 gcagaagacg cagcgcgagc cgcggttcct cgtgcaccag cgccggcgga gaaaccgtcg
     1021 tcgcagcgcg cacaagcgaa tccgaaagcc gcacaaggcg gtgcatctcg cccgacaatt
     1081 cggcggcgga gactccggcg gcggcggtgc gcaggagcca ggctcccggc gatgcgcccc
     1141 gcgcaagttc cgtgaccaac cgacgcaaac gctcgcgctc ctcgccatcg gcgatgcgcg
     1201 ccgacacggc aacgccttcg ccgccgggtt cgcgaacgag atagcgcgac gggagtcgga
     1261 tacgcgtcgt caggcgcgcg cccttgcttc cgagcggatc cttgcgaacc tgcaccagca
     1321 gcgcttcgcc ttcgcgaaga acagccgcga gcgaaagcga tggtccgttc tccgctcgcc
     1381 gcgcttcgcc gcccgtgccg tcttgcagca aggcgctgcg ccgatcgccg atgtccacga
     1441 acgccgcctg cattcccggc agcacccgca tcacccgccc gcgatagaca ttgcccacga
     1501 ggctgcgccg cccggggcgt tcgaggtaaa tctcctgcac cgcgccgcct tcgacgacgg
     1561 ccacccgggt ttcgtaaggt gcggcgttga cgacaatctc ggtactcacg gaaccagccc
     1621 cgcgtttcga agcagctgca cggtttcggc caacggcagg ccgacgacgt tggaataact
     1681 gccggcgagc cgcacgagaa aggccgaggc cgcgccctga atccggtagg cccccgccgc
     1741 atcccggccc tcgccgctcg ccacataacg tccgcgggtt gccgcatcca gcggacggaa
     1801 ccagattcgg gtgagcgagg tcgcaaccgc cgtcggacct tcccccgcaa gagcgacggc
     1861 agtcacgacg cgatgctcac ggccgcttaa ttgtgcgagc atctcctcgg ccgcctcgtc
     1921 atcggcgggc ttgccgaaaa ttgccgcgcc acaaacgacg accgtgtctg cgcccagggc
     1981 gcaggcgccg attcgctcgg ccacttccgc ggccttcgca cccgccagtc gccgtatcag
     2041 cgtttcggcg tcttcccccg ctgcgatggc ctcggaaatt cgtggagcct ccacacgaaa
     2101 ccgcagaccg atacgggcga ggagcgcgcg gcggcgcggc gaagaagagg caagcgtgag
     2161 gattgcgggc atgtcatcag cttacccgac gggcaggggc ccgctgccgg caggcgcggc
     2221 acgcttgccc ggtcagcttc ggtgataggg atgccccgca ttcaggctga ttgcacgata
     2281 gagctgttcg gcgacaatta cccgaacgag agcgtgcggc aacgtcag
//
LOCUS       DASUJG010000002         2041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1223396, whole genome shotgun sequence.
ACCESSION   DASUJG010000002 DASUJG010000000
VERSION     DASUJG010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223396"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1724)
                     /locus_tag="VFX38_00020"
     CDS             complement(<1..1724)
                     /locus_tag="VFX38_00020"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX5313286.1"
                     /translation="MTENGPARTRAYRFGPYRLDAAERRLERAGEALRLPRKAFDLLL
                     ILVAAAGRLKTREELVAGLWPDTIVGEQGLSARVYLLRRALGDEDEASRFIETVRGVG
                     YRFVAPVTVETPAAAEFPTDPAPSHRRGFRLGIGAVVVIAVAIAAGIFLWRSGRQPPA
                     IATTEVATPSIAVLPFENLSADPANAYFARGIQDEILTRLAGIGSFKVISRTSTERYA
                     NHPQDLRRIGRELGVTTVLEGSVQKSGNEVHINVQLIDARTLAHIWARSYDRKLTNVF
                     GVEGEVAGEVAEALNVRLTDPERTALAARPTENPAAYDAYLRGLAYGTRGSLDLHDDR
                     EAVHEYLRAVRLDPRFAVAWARLAMARMWIYQFYEPTPAQLDEAHAAITTALRLAPDL
                     GEAWLAEGIYRDYGKHDLAGALAAFERARERLPNSAEVLARTSYVERDLGHWKRALAL
                     QQQAAQLDPRNVGDLVQWSLTYSCLRQFSRAHAVLGHALDLAPDRAYVIATVALFYQA
                     QGDLDTADEQLRPLPFDPNSLVAAGAKIRQLLYRRDYPAAIAMLKTAIAHARPGVGNI
                     VGYYYAMLG"
BASE COUNT          353 a          636 c          700 g          352 t
ORIGIN      
        1 cccagcatcg cgtagtaata gccgacgatg ttgcctacgc cgggacgggc gtgggcgatc
       61 gcggtcttga gcatggcgat ggccgcggga tagtcgcgcc ggtataacaa ctggcgaatc
      121 ttcgcgccgg cggcaacgag cgagttcgga tcgaacggca aggggcgaag ttgctcgtcg
      181 gcggtatcga gatctccctg ggcctgatag aagagcgcga cggtggcgat gacatacgcg
      241 cggtccggcg ccaggtcgag cgcgtgcccg agcaccgcat gcgcccggga aaattgacgc
      301 agacaagaat aggtcaaact ccattggacc agatcgccga cgttgcgcgg atcgagttgc
      361 gcggcctgtt gctgaagtgc gagcgcgcgc ttccagtggc cgagatcacg ctcgacgtaa
      421 ctcgtgcgcg cgagcacttc ggcgctgttg ggcagccgtt cgcgggcccg ctcgaaggcc
      481 gcgagtgcgc cggcgagatc gtgcttgccg taatcgcgat aaattccttc ggcgagccag
      541 gcttcgccga gatcgggcgc aagcctgagc gccgtcgtga tcgccgcgtg cgcctcgtca
      601 agttgcgcgg gggtcggttc gtaaaattga taaatccaca ttcgcgccat ggcgagacgg
      661 gcccaagcca ccgcgaagcg gggatcgagc ctcacggccc gtaggtactc atgcacggct
      721 tcgcgatcgt cgtgcagatc gagcgagccg cgggtaccgt aggcgaggcc gcgcagatag
      781 gcgtcgtagg cggcgggatt ttcggtcggc cgggcggcga gggcggtgcg ttcgggatcg
      841 gtaagccgga cgttgagcgc ctcggctacc tcgcccgcga cttcgccctc gacgccgaag
      901 acgttggtga gtttgcggtc gtaactccgg gcccagatgt gggccagcgt tcgggcatca
      961 atcaattgga cgttgatgtg cacctcgttg cccgatttct gcacgctgcc ttcaagcacg
     1021 gtcgtaacgc cgagttcgcg gccgatgcgc cgcaggtctt gcgggtggtt cgcgtagcgt
     1081 tcggtcgagg tgcgcgagat gaccttgaag gagccgatgc cggcgaggcg cgtgagaatc
     1141 tcgtcctgga tgccgcgggc aaagtaggcg ttggccggat cggcggagag attctcgaag
     1201 ggcagcaccg cgatcgatgg cgtggccacc tctgtcgtcg cgattgcggg cggctggcgg
     1261 ccggaacgcc agaggaagat tccggcggcg atggcgacgg cgatgacgac gactgcgcca
     1321 atgccaaggc ggaaaccccg ccgatgggat ggcgctgggt cggtcgggaa ttccgccgcc
     1381 gccggcgtct cgacggtgac cggcgcaacg aaacgatagc cgaccccgcg cacggtctcg
     1441 atgaatcgcg aggcctcgtc ctcgtcgccc agcgcccggc gcaacaggta cacccgggcg
     1501 ctgagcccct gttccccgac gatcgtatcc ggccacaacc cggcgaccag ctcctcgcga
     1561 gtcttcagcc gcccggctgc ggcaaccagg atcagcagca gatcgaaggc cttgcgcggc
     1621 agccgcaggg cttcgcccgc acgctccagg cgccgctcgg cagcgtccag ccgatagggc
     1681 ccgaagcgat aggcccgagt tcgcgccggg ccgttttcag tcattgctgc ccgattcccc
     1741 gacctgaaaa tgaaaatgta agggaaaaat catcgaaaca tcatgtagac ttcaggcaac
     1801 gtttcgccct cccgtctata ttttctccgt gaccagaata cgctgccgcg cgacgggtcg
     1861 ggagatgcag cgcactccgt gtcggtccat gcgatcgatc ccgacaccgg cgcgcttgcg
     1921 gaaatcgcgg gctcgccgtt ctttgccggc tccggctcgg acgcggttgc cgtcacggcc
     1981 tgcgcgtgac gaacgtattt cggggacgat tctcgttctc cattttcacc acggaggttt
     2041 c
//
LOCUS       DASUJG010000003         2511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_123019, whole genome shotgun sequence.
ACCESSION   DASUJG010000003 DASUJG010000000
VERSION     DASUJG010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123019"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VFX38_00025"
     CDS             <1..516
                     /locus_tag="VFX38_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEX5313287.1"
                     /translation="SPPLGAGERLEINYNQRNVAVRGREPGLRLCRCGEETALADRGR
                     ELCERLAPVAAHFDAGAATREYGAALELAAARFADPDRTPSARALAEIAAAPGGFLGW
                     ALERSQRLTAALRESPLAPERLAALREEAALSLAKQAALEAASSESFEDYLARYYAGG
                     GVARASRAAAS"
     gene            650..1588
                     /locus_tag="VFX38_00030"
     CDS             650..1588
                     /locus_tag="VFX38_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5313288.1"
                     /translation="MAPVLSIHAGVEPRLAAALAAIERVVLGKPREIRLALVCLIAGG
                     HLLIEDIPGVGKTTLARALARVLGLAFQRIQFTSDLLPADILGATVFEREQERFVFRP
                     GPLFTQVVLADEINRATPRTQSALLEAMQEGQVSVDGEARALGRPFFVIATQNPQEQI
                     GTYPLPESQLDRFAMRLALGYPDVTAERMLYQAEDRRDLLAQLPPVLDAEEVLALQQA
                     ANGVRTAPALLDYVQALVGWTRHSGRCDPGLSPRAGRVLLALARAHALVAGRPAALPE
                     DVQAVLVPAVAHRLQRHGTTGGGALARELLETVSIP"
     gene            1675..>2511
                     /locus_tag="VFX38_00035"
     CDS             1675..>2511
                     /locus_tag="VFX38_00035"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX5313289.1"
                     /translation="MERAGARRALRAPWPSRILRGQVFILPTRFGWLGVATTLVLLLL
                     GLNYENAPIFLLAFLFAALGPVSMVATHRHLQGLELGAPAAAPVFAGDPIRVRIPVVN
                     VSARRRRALIAFADEALAPALDLAPGAEGCFELVLPARERGRHRLRGLGIASSEPFGA
                     FRAWARIADTNLECIVYPRPANRAPLSGGDADKLGGMRSADQPEDFVGLAAYRPGDRP
                     GQIAWRAYARGGEIERKEFGGQGESSLWFDFDSLPDLAPELRLRVLARWLLDAERGGA
                     AAY"
BASE COUNT          320 a          921 c          870 g          400 t
ORIGIN      
        1 agcccgccgc tcggcgcggg cgagcggctc gaaatcaact acaaccagcg caacgtcgcg
       61 gtgcggggac gcgagcccgg tctgcggctg tgtcgctgcg gcgaggagac ggcgctcgcg
      121 gatcgcggcc gcgaactctg cgaacggctc gcgccggtcg ccgcgcattt cgatgccggt
      181 gccgcgacgc gggagtacgg cgcggcgctg gagttggccg cggcgcgttt cgccgatccc
      241 gatcgcacgc catcggcccg ggcgctggcg gagatcgccg cggcgccggg aggctttctc
      301 ggctgggcgc tcgagcgttc gcaacggctt acggccgccc tgcgcgaaag cccgctcgct
      361 cccgaacgcc ttgcggcgct gcgcgaggag gccgcgctgt cgctcgcgaa acaggcggcg
      421 ctcgaggccg cgagcagcga aagcttcgaa gactatctcg cccgctacta cgccggcgga
      481 ggagttgcgc gcgcctcccg cgccgcggct tcctgacgct gccgcaatcg cggctctgca
      541 agggcccccg ccttcccgca actccggcga gcaccccgac ggaggcgggc gaaaggcgga
      601 tcgaagatgc gacaatgcca gcgagggttg cgccgcaacg ggagtcccga tggccccagt
      661 actcagcatt cacgccggcg tagaaccacg gctcgcggcg gctctcgccg cgatcgagcg
      721 cgtggtgctc ggcaagccgc gggagatccg tctcgccctc gtctgcctga tcgcgggcgg
      781 gcatctcctg atcgaagaca tccccggcgt cgggaagacc acgctcgccc gcgcgctcgc
      841 ccgggtgctc gggctcgcct tccagcgcat tcagttcacc agcgatcttc tgcccgccga
      901 catcctcggt gcgaccgtgt tcgagcggga gcaggaacgc ttcgtcttcc gtcccggccc
      961 gctgttcacc caggtcgtgc tcgccgacga gatcaatcgc gccacaccgc gcacgcagag
     1021 cgcgctcttg gaagcgatgc aggaaggaca ggtgagcgtc gacggcgagg cccgtgcgct
     1081 cggccgcccc ttcttcgtaa tcgccaccca gaatccgcag gagcagatcg gcacctatcc
     1141 gctgcccgaa tcgcagctcg accgcttcgc gatgcggctc gcgctcggct accccgatgt
     1201 caccgccgaa cgcatgcttt atcaggcgga agatcgccgc gatctgctgg cgcaactgcc
     1261 gccggtactc gacgcggagg aagttctcgc cctgcagcag gcagcgaacg gcgtgcgcac
     1321 ggccccggcg ctgctcgact acgtgcaggc actggtcggc tggacccgcc attcggggcg
     1381 ctgcgatccg ggactttcgc ctcgtgccgg gcgtgttctt ctcgccctgg cccgcgcgca
     1441 tgcgctcgtc gccggtcgtc cggcggcgtt gcccgaggac gtgcaggcgg tgctcgtccc
     1501 ggccgtcgcc catcgcctgc aacgccacgg aacgacgggc ggtggcgcgc tcgctcgcga
     1561 actcctcgag accgtgtcga ttccctgagt cgccgcgtgc tccgatccag gccatggcgt
     1621 gcggcttcgt ccatcggtgc ggggtcgccg ccgccgcgcc gctggcggct atggatggag
     1681 cgcgccggcg cgcggcgcgc gctacgcgca ccctggccct cccgcattct tcgcgggcag
     1741 gtgttcatcc tgccgacgcg ctttggctgg ctgggcgtcg ctaccaccct ggtgctgctg
     1801 ctgctcgggc tcaactacga gaacgcgccg atcttcctgc tggccttcct gtttgccgcc
     1861 ctcggcccgg tcagcatggt cgcgacccat cggcatctgc agggactcga actcggagcg
     1921 cccgcggccg ccccggtctt cgccggcgat ccgatccggg tgcgcattcc ggtcgtcaat
     1981 gtctccgccc gccgccggcg cgcattgatt gcctttgccg acgaggccct cgcccccgcg
     2041 ctcgatcttg cccccggggc ggagggctgc ttcgagctgg tgcttcctgc gcgcgagcgc
     2101 gggcgtcatc ggttgcgagg cctggggatc gcgagttccg agcccttcgg ggcctttcgt
     2161 gcctgggcgc gcatcgccga cacgaatctc gaatgcatcg tctatccgcg cccggcgaac
     2221 cgcgcgccgc tctccggtgg cgacgccgac aagctcggag gcatgcgctc ggccgatcaa
     2281 ccggaggatt tcgtcggact cgccgcctac cggcccggtg accgcccggg gcagattgcc
     2341 tggcgcgcct atgcccgcgg cggcgagatc gaacgcaagg aatttggcgg gcagggagag
     2401 agcagcctgt ggttcgactt cgactccctg ccggatctcg cccccgagct gcggctgcgg
     2461 gttctggcgc gctggcttct cgatgcggag cggggcgggg cggcggctta c
//
LOCUS       DASUJG010000004         3116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_979872, whole genome shotgun sequence.
ACCESSION   DASUJG010000004 DASUJG010000000
VERSION     DASUJG010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979872"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2686
                     /locus_tag="VFX38_00040"
     CDS             <1..2686
                     /locus_tag="VFX38_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX5313290.1"
                     /translation="IRITDGSANDRDPMWVGGKLYFLSDRNGPATLFGYDLRTKETRQ
                     LVANDAFQIDSASAGPGAIVYSQMGAINLYDLASGKTRAVPITIAADLPQLAPRFENV
                     AKEIQNANLSPSGVRAVFEAHGDILTVPADKGDVRDITRTPGIEERDPAWSPDGQWIA
                     YFSDASGEYALYLRRQDGLGKPRRISLGEPPSFFYQPVFSPDGKRIAYSDKRLNLWYV
                     DLDHPQPVKVDTDLYDTPLHEFDQVWSPDGQWLAYTKQLPNHLRAVFVYSLATHKARQ
                     VTDGMSDCLYPAWDASGKYLYFTASTDTALSNGWLDMTSESHPVNRNVYVAVLRKDLP
                     SPLKPESDEEKAPAKSAAAANATAGEAEASGKPASVRIDFDGLLQRTLALPIPAANYL
                     GMSAGTAGELYLLQGPQVITDFGALPMTLQKFDLAKRKSDKLADGITAFRISSDGKKM
                     LYQQNDNWYIADATAAPGPDATPIGTVGMQVYAVPKQEWAQMYREVWRIERDFFYDPH
                     FHGYDIAAAQKRFAVYLPGIASREDLNFLFRRMLSYMSVGHMFVGGGTEPDITNIEVG
                     LLGADYTSDHGRYRFAKIYNGENWNPDLHAPLTQPGVNVKPGEYLLAVNGRELHAGDN
                     LYGFFQNTVGKQTVIRVGADPGGSGARDVTVVPVASEFDLRHLAWIEDNREKVDKLSD
                     GKLAYVYLPDTAGGGYTSFNRYFFAQVGKQGAVIDERYNHGGQLADYIIDYLRRPLMS
                     WVMTREGRSYPEPAEAIFGPKAMVINQFSGSGGDALPWYFRKAGVGPLIGMRTWGGLV
                     GIGGYPPLMDGGSVTAPRWAIYGTHGEWEVENHGIAPDIEIWQDPKLVREGHDPQLEM
                     AVDTGLKQLRKHPQPTYKVPAYPNYHPVLPPLPASGGD"
     gene            complement(2934..>3116)
                     /locus_tag="VFX38_00045"
     CDS             complement(2934..>3116)
                     /locus_tag="VFX38_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX5313291.1"
                     /translation="ITFLIIAFAVFMLVRSLNKMQTRFKKPEAPAEATTKSCPQCFTD
                     IPIKATRCPNCTSTLS"
BASE COUNT          614 a         1018 c          960 g          524 t
ORIGIN      
        1 gatccggatc accgacggaa gcgccaacga ccgcgatccg atgtgggtcg gcggaaagct
       61 gtatttcctt tcggaccgca acgggcctgc aacgctcttc ggctatgacc tgcgcacgaa
      121 ggaaacgcgg cagctcgtcg ccaacgacgc gttccagatc gattcggctt ccgcagggcc
      181 gggcgcgatc gtgtattcgc agatgggcgc gatcaatctc tacgatctcg cgagcggcaa
      241 gactcgcgcg gtgccgatca cgatcgccgc cgatctgccg cagcttgcgc cgcgcttcga
      301 aaacgtcgcg aaggaaatcc agaatgccaa cctttcgccg agcggcgtgc gcgcggtatt
      361 cgaagcccac ggcgacattc tcacggtgcc cgcggacaag ggcgatgtcc gcgacatcac
      421 ccgcacgccg ggcatcgagg aacgcgaccc ggcctggtct ccagacgggc agtggatcgc
      481 ctatttctcc gatgcttccg gcgagtacgc gctgtacctc cgccgccagg acggactcgg
      541 caaaccgcgc aggatttcgc tcggcgagcc gccgtcgttt ttctatcagc ccgtcttctc
      601 gcccgacggc aagcgcatcg cctacagcga caagcgtctg aatctctggt acgtggacct
      661 cgatcacccg cagccggtca aggtggacac ggacctttac gacacgccgc tccatgaatt
      721 cgatcaagta tggtcgcccg acggccaatg gctcgcctac accaagcagt tgccgaatca
      781 tctgcgcgcg gtgttcgtgt attcgcttgc gacccacaag gcgcggcagg ttaccgacgg
      841 catgagcgat tgcctgtatc cggcctggga cgcaagcggc aagtatctct acttcaccgc
      901 gagcaccgac accgcactca gcaacggctg gctcgacatg acgagtgaaa gccatcccgt
      961 gaatcgcaac gtgtatgtcg ccgtgctgcg caaggacctg ccctcgccgc tcaaaccgga
     1021 aagcgacgag gaaaaggctc cggccaagtc tgcggcggcg gcaaacgcga cggcgggcga
     1081 ggccgaagcc tccggcaagc cggcgtcggt ccgcatcgac ttcgatgggc ttttgcaacg
     1141 cacgctcgcg ctgccgattc ccgccgcgaa ctatctcggc atgagcgccg gcacggccgg
     1201 cgaactctat ctcctccagg ggccgcaggt catcactgac ttcggcgcgc ttccgatgac
     1261 cctgcagaag ttcgatctcg cgaagcgcaa gagcgacaag ctggccgacg gcatcaccgc
     1321 cttccggatt tcgagcgacg gcaagaagat gctttaccag caaaacgaca actggtacat
     1381 cgccgacgcc accgccgcgc ccgggcccga cgcgactccg atcggcacgg tcggcatgca
     1441 ggtgtacgcc gtgccgaagc aggaatgggc gcagatgtac cgcgaagtct ggcgcatcga
     1501 gcgcgacttc ttctatgatc cgcattttca cggctacgac atcgccgcgg cgcaaaagcg
     1561 cttcgcggtg tatctcccgg gcatcgcgag ccgcgaggat ctgaacttcc tcttccgccg
     1621 catgctttcc tacatgtcgg tcggccacat gttcgtcggc ggcggaaccg aacccgacat
     1681 caccaacatc gaagtcggac ttctcggcgc ggactatacg agcgatcacg gccgttaccg
     1741 cttcgcgaaa atctacaacg gcgagaactg gaaccccgat ctgcatgcgc cgctgacgca
     1801 accgggcgtg aacgtgaagc ccggcgaata tctcctcgcc gtcaacggcc gcgaactcca
     1861 tgccggcgac aatctctacg gcttcttcca gaacaccgtc ggcaaacaga ccgtgatccg
     1921 cgtcggcgcc gatccgggcg gaagcggcgc gcgcgacgtg acggtcgttc cggtcgcaag
     1981 cgaattcgat ctccggcatc tcgcctggat cgaggacaac cgcgagaagg tggacaagct
     2041 aagcgacggc aagctcgcct acgtctatct gcccgatacg gccggcggcg gctacaccag
     2101 tttcaaccgc tactttttcg cgcaggtcgg gaaacagggc gcagtgatag acgaacgcta
     2161 caaccacggc ggccagctcg ccgactacat catcgactat ctgcgccgcc cgctgatgag
     2221 ctgggtcatg actcgcgaag gccggagcta tccggagccg gccgaggcca tcttcgggcc
     2281 gaaggccatg gtcatcaacc agttctcggg ctcgggcggc gacgccctgc cctggtattt
     2341 ccgcaaggcc ggcgtcgggc cgttgatcgg catgcgcacc tggggcgggc tggtcggcat
     2401 cggcggctat ccgccgctga tggacggggg ctccgtgacc gcgccgcgct gggcgattta
     2461 tgggacgcac ggcgaatggg aagtggagaa ccacggcatc gcacccgaca tcgagatctg
     2521 gcaggacccg aaactggtgc gcgagggtca cgacccgcag ctcgaaatgg cggtagacac
     2581 ggggctgaaa cagcttcgca agcacccgca gccgacctac aaggtaccgg cctatccgaa
     2641 ctatcatccg gtgctgccgc cgctccccgc cagcggcggc gactagtcca gcaatgaaac
     2701 gggtatccgt ccccgtttga ttgctcgcca ggggcggcac atgtcgggcc ctggctccgc
     2761 ggcttcgtcg cgccaagtca cacaccatgt aggccggcat aagaccgaag gtcgttgccg
     2821 gcaaagatgc cgggaacgct gcgcttatcc cggcctacat aaaatgcgaa cttgtgaggc
     2881 ggggcactag tgtctcgcac gtaagagggt tacgtttcag cctcgaacga atctcagctc
     2941 agggtggacg tgcagttggg gcagcgcgtt gccttgatcg ggatatcggt gaagcactgc
     3001 gggcaggact tggtggtcgc ctccgccggt gcctccggtt tcttgaagcg cgtctgcatc
     3061 ttgttaagcg aacggacgag catgaacacc gcaaaggcaa tgatgaggaa ggtgat
//
LOCUS       DASUJG010000005         3638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_735281, whole genome shotgun sequence.
ACCESSION   DASUJG010000005 DASUJG010000000
VERSION     DASUJG010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735281"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /gene="ftsA"
                     /locus_tag="VFX38_00050"
     CDS             complement(<1..1214)
                     /gene="ftsA"
                     /locus_tag="VFX38_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX5313292.1"
                     /translation="MARKTDRRLVVGLDIGTSKVVAIVGELAPDGTIEVAGVGSHPSR
                     GLKKGVVVNIESTVQSIQRAVEEAELMAGCEIHSVYAGIAGSHVRSLNSHGIVAIRDK
                     EVAPADVERVIDAARAVAIPADQRILHVIPQEFIIDHQDGIREPVGMSGVRLEARVHI
                     VTGAVSAALNIDKCVRRCGLSVDDIVLEQLASSKAVLTEDEKDLGVCLIDVGGGTTDI
                     AIFSEGGLRHTAVLPIAGDQVTNDIAIALRTPTKSAEEIKLKFACALPQLASGEETIE
                     VPSVGERPPRRLARQTLAEVVEPRYAELFALVQAEVRRSGFEDMIAAGTVLTGGAAKM
                     EGAVELAEDIFHMPVRLGAPTGVAGLAEVVRNPIHATGVGLLIYAREALGGRRGAERQ
                     EGGGFRDLFERMR"
     gene            complement(1198..1959)
                     /locus_tag="VFX38_00055"
     CDS             complement(1198..1959)
                     /locus_tag="VFX38_00055"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEX5313293.1"
                     /translation="MRAEAATLSSWRRSHRSRRWIVFLACGIALAVFLRLMLGMPAGP
                     AVTRIVVTVPPHSPVAAGVVEQAAAAAIGSGIFTVRLAAVQARIERLPWVAKAEVRRV
                     WPDALAVTVTLERPVARWGEGALVDASAHLFRPPQVTGLGMLPALSGPPGTAAQLLDD
                     LARARAVVAARGLQVTALRENARGELRVVFAGGLVLELGRERPLAKLARFERVAAPAL
                     GTALARAAAIDLRYPNGFAVSWRKATDGRKHGQKD"
     gene            complement(1949..2896)
                     /locus_tag="VFX38_00060"
     CDS             complement(1949..2896)
                     /locus_tag="VFX38_00060"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX5313294.1"
                     /translation="MSVERDPGRFGRVGVLLGGNSAEREVSLLSGGRVLEALLAAGVD
                     AHPLDPREDGLAALWERRFDRCFLVLHGRGGEDGAMQGFLETLAIPYTGSGVLGSALA
                     MDKLATKRIWRALGFPTPEWVAMRRGETPPADLFDRLGPVLAVKPAREGSTLGLSKVT
                     RSEALSAALEAAFARDADVVIEPWIAGRELTASLLGEEALPLIHIEPAVDFYDYEAKY
                     LSDATRYHCPSGLAAAEETRLRSLCRDAFLALGCSGWGRVDFIRDADGQSWLLEANTI
                     PGMTKHSLVPMAARVAGTDFQALVLRILETSMTERADAR"
     gene            complement(2893..>3638)
                     /locus_tag="VFX38_00065"
     CDS             complement(2893..>3638)
                     /locus_tag="VFX38_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX5313295.1"
                     /translation="ADEPLLERAAQTLGRPVSTSGFGPADFRASGLRPDGGRMHFTVE
                     RPGADALALTLNLPGRHNVLNALAAVAVAAELNIADEALARAFDGFHGIRRRIQNLGE
                     LRFGARRALLVDDYAHHPRELAATLEAARSAWPGRRLVVAFQPHRYSRTHDLLDDFAA
                     VLADVGVLVLGEVYAAGEKPIAGADSRALSRAIRGRGRIEPVFLPDFAALPATLDALV
                     EDGDLVLTLGAGDIGQHARELLRARRVAA"
BASE COUNT          611 a         1272 c         1236 g          519 t
ORIGIN      
        1 cgcatccgct cgaaaagatc gcggaagccg ccgccctcct ggcgttcggc gccacgccgc
       61 ccgccgagcg cctcgcgcgc gtaaatgagc aaccccacac cggtcgcgtg aatcggattg
      121 cgcacgacct cggcgagccc ggcgacgccg gtcggcgccc ccagtcgcac cggcatgtga
      181 aagatgtctt cggccagttc caccgcgccc tccatcttgg cggcgccacc ggtgagcacg
      241 gtgccggccg cgatcatgtc ttcgaagccg ctgcgccgga cctccgcctg cacgagcgcg
      301 aacagctcgg cgtaacgcgg ctccaccacc tcggcgaggg tctggcgcgc gaggcggcgc
      361 ggcgggcgct cgccgacgct cggcacctcg atggtctcct cgcccgaggc gagctgcggc
      421 agcgcgcagg cgaacttgag cttgatctcc tcggcgctct tggtcggagt ccgcagcgca
      481 atcgcgatgt cgttggtgac ctgatcgccg gcgatcggaa gtaccgccgt atggcgcaac
      541 ccgccttcgg agaagatcgc gatgtcggtg gtgccgccgc cgacgtcgat cagacagacg
      601 ccgagatcct tttcgtcttc ggtgagcacc gccttgctcg aggcgagttg ttcgagcacg
      661 atgtcgtcca ccgaaagccc gcagcggcgc acgcacttgt cgatgttgag cgccgcggac
      721 accgcgccgg tcacgatgtg cacgcgcgcc tcgagacgca ctcccgacat gccgaccggc
      781 tcgcggattc cgtcctgatg atcgatgatg aattcctgcg gaatgacgtg aaggatgcgc
      841 tggtcggcgg ggatcgcgac ggcgcgcgcg gcgtcgatca cgcgctccac gtcggccggc
      901 gcgacttcct tgtcgcggat agcgacgatg ccgtgcgaat tgaggctgcg tacatggctg
      961 ccggcaatgc cggcatagac cgaatgaatt tcgcagccgg ccatgagttc ggcctcttcg
     1021 accgcgcgct gaatcgactg cacggtagat tcgatgttga cgacgacgcc ctttttcagc
     1081 ccgcgcgagg gatgcgagcc gacgcccgca acctcgatcg ttccatcggg cgcgagttct
     1141 ccgacgatcg ccaccacctt ggaggtgccg atatccaatc ccaccaccag tcgccgatca
     1201 gtctttctgg ccatgtttcc tcccgtcggt agccttgcgc caggaaacgg cgaatccgtt
     1261 cggatagcgc agatcgatcg ccgccgcacg cgcaagcgcg gtgccgagcg ccggcgcggc
     1321 gacgcgctcg aagcgcgcga gcttcgcgag cgggcgttcg cggcccagtt cgagcacgag
     1381 gccgcccgcg aagacgaccc gaagttcgcc gcgggcgttc tcgcgcagtg ccgtaacctg
     1441 caatccccgc gcggcgacga cggctcgcgc ccgggcgaga tcgtcgagca attgcgccgc
     1501 cgttcccggc ggtccggaaa gcgccggcag catcccgagc cccgtgacct gtggcgggcg
     1561 gaacaggtgc gccgaggcat ccacgagcgc gccctcaccc cagcgcgcga ccggccgctc
     1621 gagcgtcacg gtgaccgcga gcgcatcggg ccagacgcgg cgcacctcgg ccttcgcgac
     1681 ccagggaaga cgctcgatgc gcgcctgcac cgccgccagc ctgacggtga aaatcccgct
     1741 tccgatcgcg gcagcggccg cctgctcgac aaccccggcc gcaaccggcg aatgcggtgg
     1801 cacggtgacg acaatgcggg tgacggcggg tccggcgggc atgccaagca tcaggcgcag
     1861 aaaaaccgcg agcgcgattc cgcaggcgag aaatacgatc cagcggcggc tgcgatggct
     1921 gcgccgccag gacgaaagcg tcgcggcctc agcgcgcatc ggccctttcc gtcatcgagg
     1981 tttcgagaat ccgcagcacc agcgcctgga aatccgtgcc ggcgacgcgc gccgccatgg
     2041 ggacgagact gtgcttggtc attccgggaa tcgtgttcgc ttcgagcagc caggactggc
     2101 cgtcggcgtc gcgaatgaaa tccacccgac cccagccgct gcagccgagg gcgagaaagg
     2161 cgtcacggca aagagaacgc agccgcgttt cttccgcggc cgcgagtccg ctcggacagt
     2221 gataacgggt ggcatcggac aaatacttcg cctcgtaatc gtagaaatcc acggccggct
     2281 cgatgtgaat gagcggcagg gcctcctcgc caagcagcga cgcggtcaat tcgcgaccgg
     2341 caatccacgg ctcgatcacg acatcggcgt cgcgtgcaaa ggcggcctcg agcgcggcgg
     2401 aaagtgcttc gctgcgcgtc accttgctca gtcccagggt cgagccttcg cgcgcgggct
     2461 tcacggccag gaccggcccg agacgatcga agagatccgc gggcggagtc tcgcctcgcc
     2521 gcatggcgac ccattcggga gtcgggaatc cgagcgcgcg ccagatgcgc ttggtcgcga
     2581 gcttgtccat ggcgagcgcc gaccccagga ctccgcttcc ggtgtagggg atggcgaggg
     2641 tttcgagaaa tccctgcatc gcgccgtcct ctccgcctcg gccgtgcagc acgagaaagc
     2701 agcgatcgaa gcgtcgctcc cacagggccg cgagtccgtc ctcgcgcgga tcgagcggat
     2761 gcgcatcgac gcccgcggcg agcagcgctt cgagcacgcg cccgccgctc aggagcgaca
     2821 cttcgcgttc ggccgaattg ccgccgagaa gtacgccgac acggccgaag cgcccgggat
     2881 cgcgttccac gctcatgcgg caacccgtcg cgcacgcagc agttcgcgcg catgctggcc
     2941 gatgtcgccc gcgccgagcg tgagcacgag gtcgccgtct tcgacgagcg cgtcgagcgt
     3001 cgcgggaagc gcggcaaagt cgggaagaaa cacgggttcg attcgtccgc gcccgcgaat
     3061 cgcgcgcgag agggcgcgcg aatccgcgcc ggcgatgggt ttttctccgg ccgcgtacac
     3121 ctcgccgagc acaagcacgc ccacgtccgc gagcaccgcg gcgaaatcgt cgagaagatc
     3181 gtgcgtgcgc gaatagcgat gcggctggaa cgccaccacg agacgtctcc ccggccaggc
     3241 ggagcgcgcc gcctccaggg tcgccgcgag ttcccgcgga tgatgcgcgt aatcgtccac
     3301 gaggagcgca cgccgggcgc caaagcgcaa ctcgccgaga ttctggatgc gccggcggat
     3361 gccgtgaaat ccgtcgaagg cacgcgcaag tgcttcgtcg gcgatgttga gctccgccgc
     3421 caccgcaacg gcggcgagcg cgttgagcac gttgtgccga ccgggcagat tgagcgtgag
     3481 ggcgagcgca tcggcgccgg gacgctcgac ggtgaaatgc atgcgcccgc cgtcgggccg
     3541 cagcccgctc gcgcggaagt cggccgggcc gaaaccggag gtcgaaaccg gccgcccgag
     3601 cgtttgcgcg gcgcgttcga ggagcggctc gtccgcac
//
LOCUS       DASUJG010000006         2577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_980426, whole genome shotgun sequence.
ACCESSION   DASUJG010000006 DASUJG010000000
VERSION     DASUJG010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980426"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="VFX38_00070"
     CDS             complement(<1..1160)
                     /locus_tag="VFX38_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5313296.1"
                     /translation="MKVPEILLVESEHAPGSLARILQAIGESGLVVEELIAVRRDQDK
                     TVWEITLEMDEHTDYGLAERIAALPNARLLGESDRVFDRHRGGKIRTVARTPVKSLQE
                     LRDIYTPGVARVCLAIQRDPDKVREYTNLPRTVAIVTNGTAVLGLGDIGAQAGLPVME
                     GKAALMAQFAELSGVPILLGTRDPAEVIATVKAIAPSFGAIQLEDIAAPACFEVEGAL
                     RDALDIPVMHDDQHGTAVIVLAALHNATRRVGRELCGSVIGQIGLGAAGIGIARLLMA
                     YGVKTVIGSDPQPLARERLESMGGRAAPLEELMRNAEIVIATSGRPGLIEPAWIRRGQ
                     IILALSNPEPEIEAADALAQGAAFAADGRAINNLLGFPGLFRGALEAGAKRFT"
     gene            complement(1180..2292)
                     /gene="mnmA"
                     /locus_tag="VFX38_00075"
     CDS             complement(1180..2292)
                     /gene="mnmA"
                     /locus_tag="VFX38_00075"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX5313297.1"
                     /translation="MSAPLAIVGLSGGVDSAVAAHLLLKAGWRVEGLHMVNWEADDAW
                     CSRVDDEAAAAAVADHLGIVLHRANFAREYRERVFADFLAEYRAGRTPNPDVLCNRDI
                     KFGAFFSWALRLGADAVATGHYAGIGTGEHPTLLCARDSNKDQTYFLHAIRGETLART
                     IFPLGEYRKPEVRAIAERIGLPNHDRPDSTGICFIGERPFAAFLENYLPPARGRIEDE
                     DGRAIGEHRGLHFYTLGQRTGLGVGGRRNAREAPWYVIEKNMARNALMVAQDADHPRL
                     LATALEADRLQWIAGEPPSSPAAVRVRVRHRQPPVPATLDMRGETMGLRFAQPLRAVT
                     PGQYAVIYAGDVCLGGGRIIARDTLADHLGRRRATG"
     gene            complement(2289..>2577)
                     /locus_tag="VFX38_00080"
     CDS             complement(2289..>2577)
                     /locus_tag="VFX38_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX5313298.1"
                     /translation="ETGYRFVPEALGGVYVWRQPGDGDTFLRCNFIGRCTGHDPRAKL
                     DDGIIGPEWLALAELAAQAGALRSPLVLRSFREYLAGVRYPLEVLTTLIEA"
BASE COUNT          429 a          819 c          902 g          427 t
ORIGIN      
        1 gtgaatcgtt tcgcgccggc ttcgagcgcc ccgcggaaga gaccggggaa accgagaaga
       61 ttgttgatcg cgcgcccgtc ggcggcgaag gcggcgcctt gcgcgagcgc atccgcggct
      121 tcgatttcgg gttcgggatt cgaaagcgcg aggatgatct ggccgcggcg gatccaggcg
      181 ggctcgatca ggccggggcg cccgctggtc gcgatcacga tctcggcgtt gcgcatcaat
      241 tcttcgagag gagcggcgcg gccgcccatg ctttcgagcc gttccctcgc cagcggttgc
      301 ggatcggatc cgatgaccgt cttcacgccg taggccatga ggagacgcgc aatcccgata
      361 cccgccgcgc cgagcccgat ctggccgatg acgctgccgc aaagttcgcg accgacgcga
      421 cgggtcgcgt tgtgcagcgc cgcgagcacg atcacggccg tgccgtgctg gtcgtcgtgc
      481 atgacgggaa tgtcgagcgc atcgcgaagc gcgccctcga cctcgaaaca ggcgggcgcg
      541 gcaatatctt cgagttgaat cgcgccgaag gagggcgcga tcgccttgac cgtggcaatg
      601 acttcggcgg gatcgcgggt cccgagcaag atcggcacgc cggaaagctc ggcgaactgc
      661 gccatcaatg cggccttccc ttccatgacg ggaaggccgg cctgggcgcc gatgtcgccg
      721 aggccgagga ccgccgtacc gttggtgacg atggcgacgg tgcgaggcag attggtgtat
      781 tcgcgaactt tgtccgggtc tcgctgaatt gcgaggcaga cgcgggcaac gcccggcgtg
      841 taaatgtcac gaagctcttg caggcttttg accggcgtgc gcgcgaccgt gcgaatcttc
      901 ccgccgcgat ggcgatcgaa cacgcggtcc gattcgccga gaaggcgcgc gttgggaagg
      961 gccgcgatcc gttcggcaag tccgtagtcg gtgtgctcgt ccatttcgag cgtgatttcc
     1021 cagacggtct tgtcctggtc gcggcgcacc gcgatcagct cttcgacgac gagcccggat
     1081 tcgccgatgg cctggagaat gcgcgcaagg ctcccgggcg catgctcgga ttcgacgaga
     1141 aggatttcgg ggactttcat gtcttttctc tccgtcggat cagcccgtgg cgcgtcgccg
     1201 cccgagatga tcagcgagtg tatcgcgggc gatgatgcgc ccgccgccga gacagacatc
     1261 gccggcgtag attaccgcgt actgccccgg cgtcaccgcc cgcaggggct gggcgaaacg
     1321 caggcccatg gtctcgcccc gcatgtccaa agtcgccggc accggcggct ggcgatggcg
     1381 cacgcgaacc cgcaccgccg cgggcgaaga gggcggctcg cccgcaatcc attgcaagcg
     1441 atcggcttcg agcgcggtcg cgaggagccg cggatgatcg gcatcctgcg cgaccatcaa
     1501 ggcattgcgc gccatgttct tttcgatgac ataccacggc gcctcgcggg cgttgcggcg
     1561 gccgccgact ccgagtccgg tccgctggcc gagcgtgtaa aagtgcaagc cccgatgttc
     1621 tccgatcgcg cggccgtcct cgtcttcgat ccgtccccgt gccggcggaa gataattctc
     1681 gaggaacgcg gcgaaggggc gttcgccgat gaagcagatt ccggtggaat cggggcggtc
     1741 atgattggga aggccgatcc gttccgcgat tgcgcgcacc tcgggcttgc gatattcgcc
     1801 gagcgggaaa atcgtgcgcg cgagtgtttc gccgcggatg gcatgcagaa aataggtttg
     1861 atccttgttc gaatcgcgcg cgcacagaag cgtggggtgc tctcccgttc cgatacctgc
     1921 gtaatgtccg gtcgcgacgg cgtcggcgcc gaggcgcagc gcccaggaaa agaacgcgcc
     1981 gaacttgatg tcgcgattgc agagcacgtc gggattggga gtgcgcccgg cgcggtactc
     2041 ggcgagaaaa tccgcgaaca cccgctcgcg gtattcgcgc gcgaagttgg cccgatgcag
     2101 cacgatgccg agatggtcgg cgacggcggc cgcggcggct tcgtcgtcca cgcggctgca
     2161 ccaggcgtcg tccgcttccc agttgaccat gtgcaggcct tcgacccgcc agccggcttt
     2221 gagcagcaga tgcgcggcga ccgcggagtc cacaccgccg gaaagaccga cgatggcgag
     2281 cggtgcgctc atgcctcgat cagggtcgtg agtacttcga gcggatagcg cacgccggcg
     2341 agatactcgc ggaaggagcg aagcacgagc gggctgcgca atgcgccggc ttgcgcggcc
     2401 agttcggcga gcgcgagcca ctcgggcccg atgatcccgt cgtccagttt cgcccgcgga
     2461 tcgtggccgg tacagcgacc gatgaagttg cagcgcagga aggtgtcgcc gtcgcccggc
     2521 tggcgccaga cgtacacgcc gccgagcgcc tcgggaacga agcgataacc ggtttcc
//
LOCUS       DASUJG010000007         2183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1103017, whole genome shotgun sequence.
ACCESSION   DASUJG010000007 DASUJG010000000
VERSION     DASUJG010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103017"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..1256
                     /gene="purM"
                     /locus_tag="VFX38_00085"
     CDS             222..1256
                     /gene="purM"
                     /locus_tag="VFX38_00085"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX5313299.1"
                     /translation="MAQDSPRYRAAGVDIDAGDRLVERIKPLAAATLRPEVLAGPGGF
                     ASLFRLPADRYRDPVLVAGTDGVGTKLLLALAADRLEGLGRDLVAMCANDVAVTGAEP
                     LFFLDYYATGALDVAQGERLIAGIAEGCREAGCALVGGETAELPGLYAGRDFDLAGFC
                     VGIVERERILGATAVRSGDIVLGVASSGPHANGYSLIRRILADTGSDLGELLAGRSLA
                     DWLLEPTIIYVQAVRALLAAEPIHALAHITGGGLTENLPRVLPEGLGAIIEPRAWPRP
                     AIFTWLAEKGSIEEVELLRVFNAGIGLVAVLPADSAKSAAGILKALGLESWLIGRVER
                     GMSGVRYSVS"
     gene            1253..1909
                     /gene="purN"
                     /locus_tag="VFX38_00090"
     CDS             1253..1909
                     /gene="purN"
                     /locus_tag="VFX38_00090"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX5313300.1"
                     /translation="MKSSLPRVVVLGSGTGTNFEALAEKAGDDYEIVAVGSDRRDAPI
                     LERARRRRIPTFSCTVTDFPESARHDSQLASALGARRPEWVALAGYMRILGPDTLAAC
                     AGRILNVHPALLPLFPGTDTHRRALAAGASEHGATVHFVTARLDAGPAILQGRTAVRE
                     RDDPESLRLRVQALEHRIYPLALAWCARGRVLLRQNRAWFDGRPLDEPVTILEARQCA
                     "
     gene            1900..>2183
                     /locus_tag="VFX38_00095"
     CDS             1900..>2183
                     /locus_tag="VFX38_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313301.1"
                     /translation="MRMKALLACSLLILAAVAAAAPPHKPAKAELPATLAIPPFSMQY
                     QVLRNGWHMGDARFTLERDGDVWQFRSEAAPAGLAALFIHSTWSESSRFRV"
BASE COUNT          323 a          780 c          706 g          374 t
ORIGIN      
        1 cggctgcgag atcgcgggag aaggcggcgt cgcgcgcgct gtccgattga tcggcgaccg
       61 gtgtcgccgc ggcgtacaaa tccgtggcgg tcgccgccct cgctcccgcc gcgcagagca
      121 gacccgccaa caccaacaga agtcccgctc cccctcgcaa catgatcggc ccgcccaaag
      181 cccgtaaaat agcataggct ttcccgcata tcccctgcca catggcccag gactctcccc
      241 ggtatcgcgc cgcgggcgtg gacatcgatg ccggcgatcg actcgtcgag cggatcaaac
      301 ccctggccgc ggcgaccctt cgtcccgagg ttctcgccgg gcccggaggt ttcgcgagcc
      361 tgttccgcct gcctgccgat cgctatcgcg atcccgtcct cgtagccggc accgacggcg
      421 tcggcaccaa acttttgctc gccctcgccg ccgatcggct cgagggactc ggacgcgatc
      481 tggtcgcgat gtgtgccaac gacgtcgccg ttaccggcgc cgagcctctg ttctttctgg
      541 attattacgc caccggagcg ctcgatgtcg cccagggaga acggctgatc gccggcatcg
      601 ccgaaggctg ccgggaggcg ggatgcgccc tggtcggcgg cgaaaccgcg gaactgccgg
      661 ggctctacgc gggccgggac tttgatctcg cgggattctg cgtcggtatc gtggagcgcg
      721 agcgcattct cggcgccacc gccgtccgct ccggcgatat cgtgctcggc gtcgcctcca
      781 gtggcccgca cgcgaacggc tactcgctga tccgtcgcat tctcgccgat accggctccg
      841 acctcgggga attactcgcg ggccgttccc ttgccgactg gctgctcgaa ccgacgatca
      901 tttacgtgca ggcagtccgc gctctgctcg cggcggagcc cattcacgcc ctcgcgcata
      961 tcaccggcgg cgggctcacg gaaaacctgc cgcgggtgct gccggaaggc ctcggagcga
     1021 tcattgaacc gcgcgcctgg ccgcggccgg cgatcttcac ctggctcgcg gaaaagggtt
     1081 ccatcgagga ggtcgaactg ctgcgggtct tcaatgccgg gatcggactc gtcgccgttc
     1141 tgccggccga ttcggcgaag agcgcagcgg gcatcctcaa agccctggga ttggaatcct
     1201 ggctaatcgg cagagtcgaa cgcggtatgt ccggcgtccg ctactcggta tcgtgaagtc
     1261 ctccctgccg cgggtcgtcg tgctgggctc ggggacggga accaacttcg aggcgctcgc
     1321 ggaaaaggcc ggcgacgatt acgaaatcgt cgcggtcggc agcgatcggc gcgatgcgcc
     1381 cattctcgag cgggcacgcc gacgccggat tccgacgttc tcctgtaccg tgaccgattt
     1441 tcccgagagc gcgcgccacg attcgcagct cgcctccgcg ctcggggccc gacggcccga
     1501 atgggtcgcg ctcgccggat acatgcggat tctcggaccg gacaccctcg ccgcctgcgc
     1561 gggacggatt ctcaacgttc acccggcgct gcttccgctc ttcccgggaa ctgacacgca
     1621 taggcgtgcg ctcgccgcag gcgcgagtga acacggcgcg accgtgcact tcgtcaccgc
     1681 ccggctggat gcgggccccg cgattctgca aggacgcacc gccgtgcggg agcgcgacga
     1741 tcccgagtcg cttcgcctgc gggtacaggc gctagagcac cggatttatc cgctcgcgct
     1801 cgcctggtgt gcccgcggtc gcgtcttgct gcggcagaat cgcgcatggt tcgacggtcg
     1861 tccactcgac gaacccgtga cgatcctcga ggcccggcaa tgcgcatgaa ggccctgctc
     1921 gcctgctcgc tcctgatcct tgccgcagtg gccgcggccg ccccgccgca caaaccggcg
     1981 aaagcggaac tgccggcgac gctcgccatt ccgccgtttt ccatgcagta ccaggtgctg
     2041 cgcaacggct ggcacatggg ggatgcacgg ttcaccctcg agcgcgacgg cgacgtctgg
     2101 cagtttcgtt ccgaggcggc accggccggt ctcgcggcgc tcttcatcca ttccacctgg
     2161 agcgaatcga gccgctttcg cgt
//
LOCUS       DASUJG010000008         2250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_613825, whole genome shotgun sequence.
ACCESSION   DASUJG010000008 DASUJG010000000
VERSION     DASUJG010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613825"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VFX38_00100"
     CDS             <1..340
                     /locus_tag="VFX38_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313302.1"
                     /translation="YHHLGIAEDNLPPNIGRLYHVGGARRKMPGLEKFLRSVVVRGLI
                     RLAVSKRRYKRMQMELERWNARPGIIVVPGETYDALANHYAGGVEELARLCGRRPRWF
                     EHRGEPSIAL"
     gene            complement(421..1842)
                     /locus_tag="VFX38_00105"
     CDS             complement(421..1842)
                     /locus_tag="VFX38_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017940774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX5313303.1"
                     /translation="MNEDAAPATESSARRLFVLDTNVLMHDPASLFRFAEHDIFIPMV
                     VLEELDANKKGVSEPARNVRQASRFLDELVRDRDRGEIEAGIALDPVANGNGRHAGGR
                     LFLQTAPLEPHNATVLAADKADNQMLATTLALAERHPERVTLVSKDINLRIKAAVLGL
                     KVEDYYNDKVLDDSDLLYRGVEMLPDDFFADAGRRVESWHAEGRTYYRLPLPVFPVRA
                     NQFLYSEGEGEGMELRVRDIAADSFTAELAWDYRRERHAVWGVAARNREQSFALNLLM
                     NPELDFVTLIGPAGTGKTLLALAAGLAQTLDDKRYREIVMTRVTVPLGEDIGFLPGTE
                     EEKMTPWMGALMDNLEVLTTSEEGGTWGRGATQDLLASRIKIRSLNFMRGRTFLNRYI
                     IIDEAQNLTAKQLRALVTRAGPGTKMIALGNIAQIDTPYLSETTSGLTYVVERFQDWP
                     YAGHITLQRVERSRLADYATEIL"
     gene            complement(1839..>2250)
                     /locus_tag="VFX38_00110"
     CDS             complement(1839..>2250)
                     /locus_tag="VFX38_00110"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX5313304.1"
                     /translation="GELSDNDLAGRKTVFYFYPKDDTPGCTIEGNEFRDLHEKFRRAG
                     VAVYGVSRDNLRSHERFRDKFDYRFELVSDPEEKLCEAFGVMRDKNMYGKKVRGIERS
                     TFLFDSKGTLRQQWRKVKPEGHAAEVLEAAKALD"
BASE COUNT          371 a          671 c          748 g          460 t
ORIGIN      
        1 ctatcaccat cttggtatcg ccgaagacaa tttaccgccg aatatcggac ggctttatca
       61 cgtcggcggc gcgcggcgca aaatgcccgg gctggaaaaa tttctgcgct cggtcgtcgt
      121 gcgcgggctg atccgtctcg cggtatccaa acgccggtac aagcggatgc agatggaact
      181 cgaacgctgg aacgcgaggc cgggcataat cgtagtgccg ggtgaaacct atgatgcctt
      241 ggcgaatcat tatgcggggg gcgtcgagga actcgctcgc ctgtgtggca ggcgtccgcg
      301 ttggttcgaa catcgcggtg aaccctccat cgccctctag cagccgcctt gtagcgtagg
      361 tgtaggagcg agcaagctcg ctcctacacg tctctgtcgg ttaggagatg atgtgcgccg
      421 ctacaggatt tcggtggcgt agtcggcgag acgcgaacgt tcgacccgtt gcagcgtgat
      481 gtgcccggcg tagggccagt cctggaagcg ctccaccacg taggtcagcc ccgaggtcgt
      541 ttcggacaga tacggcgtat caatctgggc gatgttgccg agcgcgatca tcttggtgcc
      601 cggcccggcg cgggtgacca gcgcgcgcag ctgcttggcg gtgaggtttt gcgcctcgtc
      661 aatgatgatg tagcggttga gaaaggtacg tccgcgcatg aagttgagcg agcggatctt
      721 gatccggctg gcgagcagat cctgcgtcgc cccgcggccc caggtgccgc cttcctcgga
      781 cgtggtcagc acttcgaggt tatccatcaa cgcgcccatc cacggtgtca tcttttcttc
      841 ttcggtgccg ggcagaaagc cgatgtcctc gccgagcggc acggtgacgc gggtcatgac
      901 gatttcccgg tagcgcttgt cgtcgagcgt ctgcgcgagt ccggcggcga gcgcgagcag
      961 cgttttcccg gtgccggccg gcccgatcag ggtcacgaag tcgagttcgg gattcatcag
     1021 caaattgagc gcgaagctct gctcgcggtt gcgcgccgcc actccccaaa ctgcgtggcg
     1081 ttcgcgccgg tagtcccagg cgagttcggc ggtgaaggaa tcggcggcga tgtcgcgcac
     1141 acgaagttcc attccttcgc cttcgccttc actgtagaga aactggttcg cacgcaccgg
     1201 aaagacgggc agcggcaggc ggtagtatgt gcgcccttcg gcgtgccagg actcgacccg
     1261 acggccggcg tcggcgaaga aatcgtccgg aagcatttcc acgccgcggt agagaagatc
     1321 gctgtcgtcg agcaccttgt cgttgtaata gtcttcgacc ttgagtccga gcaccgcggc
     1381 cttgatgcgc aggttgatgt ccttggacac cagcgtgacc cgctcgggat ggcgttcggc
     1441 gagcgcgagc gtggtcgcga gcatctggtt gtcggccttg tccgcggcca gcaccgtggc
     1501 attgtgcggt tcgagcggcg cggtctgcag aaacaggcgc ccgcccgcat ggcggccgtt
     1561 gccgttggcg accggatcga gcgcgatgcc ggcctcgatt tcgccccggt cgcggtcgcg
     1621 gaccagctcg tcgagaaagc ggcttgcctg acggacgttg cgcgccggct ccgacactcc
     1681 cttcttgttc gcgtcgagtt cttcgagcac gaccatcgga atgaaaatgt cgtgctcggc
     1741 gaagcggaag agcgaggccg gatcgtgcat gagcacgttg gtgtcgagca cgaacagccg
     1801 tcgcgcggag gactccgtcg ccggcgcagc gtcttcgttc aatcgagcgc tttggcggct
     1861 tccagcactt ccgcggcgtg tccttcgggt ttgaccttgc gccattgctg tctcagcgtc
     1921 cctttggaat cgaagagaaa cgtgctccgt tcgatgccgc gcaccttctt gccgtacatg
     1981 tttttgtcgc gcatcacgcc gaaggcctcg cagagttttt cctcgggatc ggagacgagt
     2041 tcgaagcggt aatcgaactt gtcgcggaag cgttcgtgcg agcgcaggtt gtcgcgcgac
     2101 acgccgtaga cggcgacccc ggcacggcgg aacttttcgt gcagatcgcg gaattcgttg
     2161 ccctcgatgg tgcagccggg ggtgtcgtcc ttcggataga agtagaacac ggttttccgg
     2221 ccggcgagat cgttgtcgct cagttcgccg
//
LOCUS       DASUJG010000009         2294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_492003, whole genome shotgun sequence.
ACCESSION   DASUJG010000009 DASUJG010000000
VERSION     DASUJG010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492003"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..144
                     /locus_tag="VFX38_00115"
     tRNA            71..144
                     /locus_tag="VFX38_00115"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:103..105,aa:Gln,seq:ttg)
     gene            204..1140
                     /locus_tag="VFX38_00120"
                     /pseudo
     CDS             204..1140
                     /locus_tag="VFX38_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637112.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
     gene            1296..1994
                     /locus_tag="VFX38_00125"
     CDS             1296..1994
                     /locus_tag="VFX38_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018169038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEX5313305.1"
                     /translation="MAVEFMLNAEPRTDAGKAASRRLRRDGRVPAVLYGGAQGAQALS
                     LDHNELMQNLKSEAFRSRVLTVRFGGASAQAIVKDLQAHPFKSEVLHVDLQRVDAAQK
                     IRMEVPLHFKGGDVAPGVKAGGVLSRSVVEVEIECLPKDLPEHLELDVAALDIGDALH
                     LSDIPLPAGVAIVALAHDDVHEHDVPVASVHLPRVTAEEEEAEAQAAIEGAEAAPAAD
                     AKGREESRKGESAA"
     gene            2016..>2294
                     /locus_tag="VFX38_00130"
     CDS             2016..>2294
                     /locus_tag="VFX38_00130"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Enables the recycling of peptidyl-tRNAs produced at
                     termination of translation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX5313306.1"
                     /translation="MSENAIRVVVGLGNPGAEYLRTRHNIGFWFADTLAAELGVGFRD
                     THRFHGKTARAKFGGAELLLLKPDTLVNRSGLAVRAVLDYFKHEPGEVL"
BASE COUNT          402 a          760 c          749 g          383 t
ORIGIN      
        1 gggcgatcct cgcccgcggc tgcaatcgct cgccgcttcg cgagcgcctg cgaggcgaac
       61 cgggaaatgt tggggcgtag ccaagcggca aggcacgggg ttttgatccc cgcatcccag
      121 gttcgaatcc tggcgcccca gccactgtct cgactgaagg ccgggaggaa cgcgacatga
      181 aggattccac ggtaccggga aacatgatgg tcttcgccgg gcgggccaat cccgggctcg
      241 cggcggacat cgcctcgcat ctgcagttgc cgctcggaaa gatcaacgtc gggcgcttca
      301 gcgacggcga agtgcaggtg gaaatcttcg agaacgtgcg cgggcgcgat gtcttcgtgg
      361 tgcaaccgac ctgcccgccg gtgaacgaaa atctgatgga gctgctgctg attgccgacg
      421 ccctgaagcg tgcctccgcg gggcggatca cggcggtcgt tccctatttc ggctactcgc
      481 gccaggaccg tcgtccgcgc tcgctgcgcg tgccgatctc ggcgaagctc gtcgccaatc
      541 tcatcgccca tgccggggtc gaccgcctgt tgagcgtcga catccacgcc gaccagattc
      601 agggctactt cgacattcct tttgacaacg tccacgcctc gccgatcctg ctctccgatc
      661 tgtggcgctg cggctatccc gacatcatca tcgtctcgcc cgacgtcggt ggcgtcgccc
      721 gcgcgcgctc ttgccaagcg catggacgat gccgatctcg cgatcatcga caagcgccgc
      781 tcgcaaccga acgaatcgaa ggtgatgaac atcatcggcg atgtccgcgg gcgcaactgc
      841 gtgatcgtgg acgacatggt ggataccgcc ggaacgctct gtcaggcggc tcaggccctc
      901 aaggacgagg gcgcggtcag ggttgtcgcc tacatcaccc atccggtact ctcgggcgac
      961 gccgtctcgc gcattgccgg ctccgccctg gaccaactcg tcgtgacgga cacgatcgcg
     1021 ctgcgccccg acgccgccgc ctgcccgaag atccgccagc tttcgatcgc cgacatcatg
     1081 gccgagaccc tgcggcgcat cagcgacgag gaatccgtct cctcgctcta cgtggattga
     1141 acgagaaggg aggggagaga agcgggaagg gagagaagct gaccgcgagg gccgcgtttc
     1201 gcttgtgtcg gccccggccc cggtccccca ttccccaaac cctgtaaaat atccggttcc
     1261 gactcgccga tccgcctttt cgagggaaga acgacatggc cgtggaattc atgctgaacg
     1321 ccgaaccgag aaccgacgcg ggcaaggccg cgagccgccg cctgcgccgc gacgggcggg
     1381 ttccggcggt cctctatgga ggagcccagg gcgcacaggc gctgagtctg gatcacaacg
     1441 aactcatgca gaacctcaaa agcgaggcct tccgttcgcg ggtgctcacc gtacgattcg
     1501 gcggcgcgag cgcgcaggcg atcgtgaagg atctgcaggc ccatcccttc aagtccgaag
     1561 tgctgcatgt ggatttgcag cgggtggacg cggcgcaaaa gatccgcatg gaggtgcccc
     1621 tgcacttcaa gggcggcgac gtcgcgcccg gcgtcaaggc cggcggcgtt ctttcgcgct
     1681 cggtcgtcga agtcgaaatc gaatgcctcc cgaaggatct tcccgaacat ctggagctgg
     1741 atgtcgcggc gctcgatatc ggcgatgccc tgcatctctc cgacattccc ctgccggccg
     1801 gagtcgcgat cgtcgcgctt gcccatgacg atgtgcacga acatgacgtg ccggtcgcct
     1861 ccgtgcatct gccgcgtgta acggccgagg aagaggaagc cgaggcacag gccgccatcg
     1921 agggcgccga ggccgctccc gccgcggacg cgaagggccg ggaagagtcc aggaagggcg
     1981 agtccgcggc ctaggcgggg cgcgtcgagc gcaccgtgtc ggaaaatgcg attcgcgttg
     2041 tcgtcgggct gggcaacccc ggtgccgaat atctgcgtac gcgccacaac atcggcttct
     2101 ggttcgcgga cacgctggcc gcggaactcg gcgtcggttt ccgcgatacc caccgtttcc
     2161 acggcaaaac ggcacgggcg aagttcggcg gcgcggagct gctgctgctc aagcccgata
     2221 cgctcgtcaa ccgcagcggg ctcgcggtgc gtgccgtgct cgattacttc aagcacgagc
     2281 ccggcgaagt gctc
//
LOCUS       DASUJG010000010         4058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_125988, whole genome shotgun sequence.
ACCESSION   DASUJG010000010 DASUJG010000000
VERSION     DASUJG010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125988"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VFX38_00135"
     CDS             complement(<1..285)
                     /locus_tag="VFX38_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313307.1"
                     /translation="MYSLEYRLFELMLRTISLSVTYVLLGICVLAGAWAPLNWQILPA
                     LWMYLAAAAAVGLAAGGISPRALPAWLAWPVLIVLAWAVFAYVAIALVPPP"
     gene            312..1595
                     /gene="purD"
                     /locus_tag="VFX38_00140"
     CDS             312..1595
                     /gene="purD"
                     /locus_tag="VFX38_00140"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012990301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX5313308.1"
                     /translation="MKVLIVGGGGREHALAWKTAQSPAVERVYVAPGNAGTAVEPRCE
                     NVAIAATDIAGLLEFAQKRKIDLTIVGPEAPLVAGMVDEFRAAGLACFGPTRAAARLE
                     GSKAFTKAFCERHAIPGAAYRGFRETEAAREYARKAALPLVIKADGLAAGKGVVIAET
                     QEEADRAIEAMLSGNAFGAAGREIVIEEFLRGEEASYIVLAAGEQYAALASSQDHKRI
                     GDGDTGPNTGGMGAVSPAPIIDDALAARIENDIIRPALAGLASEDMPYTGFLYAGLMI
                     GADGAPRLLEFNCRLGDPETQPILMRLESSLPELIQAALAGDLVGRKISWDSRSAVGV
                     VLAAAGYPGSIRRGDPIRGLDGVFPQDVKVFHAGTAMQNGDVVTAGGRVLCATALGKD
                     FEAAQAKAYETVEKIRFEGMQYRRDIGFRAVSDEK"
     gene            complement(1781..3037)
                     /gene="rho"
                     /locus_tag="VFX38_00145"
     CDS             complement(1781..3037)
                     /gene="rho"
                     /locus_tag="VFX38_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005003334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX5313309.1"
                     /translation="MNLSELKRKSATELSEVAEAVGLEGAARLRKQDMIFAILKGHAQ
                     NGEQICGDGVIEILSDGYGFLRSGDSSFQAGPDDIYVSPNQIRRFNLRTGDSVTGRIR
                     PPKPGERYFALLRVDTINDDRPEAAKSKILFENLTPLFPKKRFKLELGNGSTEDITAR
                     IIDLMAPLGMGQRGLIVSPPKAGKTMMLQNIAHSIAHNHPDCVLIVLLIDERPEEVTE
                     MTRSVRGEVLSSTFDEPATRHVQVAEMVIEKAKRLVEHKRDVVILLDSITRLGRAYNT
                     VVPASGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTILATALVETGSRMDDVIYEE
                     FKGTGNSEIHLDRRIAEKRVFPAININRSGTRREELLVTEEELQKIWILRKLLHPMDE
                     LAASEFLIDKLKDTKTNAEFFNSMKR"
     gene            complement(3184..3510)
                     /gene="trxA"
                     /locus_tag="VFX38_00150"
     CDS             complement(3184..3510)
                     /gene="trxA"
                     /locus_tag="VFX38_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012983830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEX5313310.1"
                     /translation="MSQNVSHTSDAAFDADVLKSETPVLVDYWAEWCGPCKMIAPVLD
                     EVAGDFKARIKVVKLNVDENAQTSRKYGIRGIPTLMLFKGGDVAAQKVGALSKSQLVA
                     FLEANL"
     gene            3914..>4058
                     /locus_tag="VFX38_00155"
     CDS             3914..>4058
                     /locus_tag="VFX38_00155"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006746307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5313311.1"
                     /translation="MQNRKLPLRVSDLALAPPIAAGVRDAGFEQLTPIQQRTLPDALA
                     GQDV"
BASE COUNT          725 a         1290 c         1324 g          719 t
ORIGIN      
        1 cggcggcggc acgagtgcaa tcgcgacata cgcaaacacc gcccaggcca gcacgatcag
       61 aaccggccac gccagccagg cgggcagtgc gcgcggcgag atgccgcccg ccgccagccc
      121 caccgcggcg gcggcggcga gatacatcca cagcgccggc agaatctgcc agttgagcgg
      181 cgcccaggcg cccgcgagca cgcagatgcc gagcagaaca taggtgacgc tgagcgaaat
      241 ggttcgcagc atgagctcga acagccgata ttcgagcgaa tacaatagcg cgaactccgc
      301 gaagagcgct catgaaggtt ctgatcgtcg gcggcggcgg acgcgaacat gccctggcat
      361 ggaagacggc gcaatcgccg gcggtcgagc gggtgtacgt cgctcccggc aacgccggca
      421 cggccgtcga gccgcgctgc gagaacgtcg cgattgcggc gaccgacatt gccggcctgc
      481 tcgagttcgc gcaaaagcgc aagatcgacc tcacgatcgt cggccccgag gcgccgctgg
      541 tcgcgggcat ggtggacgaa tttcgcgccg ccggtctcgc ctgtttcgga ccgacgcgcg
      601 cggccgcgcg actggagggt tcgaaggcgt tcaccaaggc gttctgcgaa cgccatgcga
      661 tcccgggagc ggcttatcgc ggctttcgcg agaccgaagc cgcgcgcgag tatgcgcgca
      721 aggccgcact gccgctcgtg atcaaggccg acggactcgc cgccggcaag ggcgtggtga
      781 tcgccgaaac gcaggaagaa gccgatcgcg caatcgaggc catgctttct ggcaacgcct
      841 tcggcgccgc cgggcgcgag atcgtcatcg aggagttttt gcgcggcgaa gaggcgagct
      901 acatcgtgct cgcggccggc gagcaatacg cagcactcgc aagttcacag gatcacaaac
      961 gcatcggcga cggcgacacc ggcccgaaca ccggcggcat gggcgccgtc tcgcccgcgc
     1021 cgatcatcga cgatgcgctc gccgcgcgga tcgagaacga catcatccgt ccggcgctcg
     1081 cggggcttgc ctccgaagac atgccgtaca ccggttttct gtacgcgggg ctcatgatcg
     1141 gcgcggatgg tgcaccgcgg ctgctcgaat tcaactgccg cctgggcgat cccgaaacgc
     1201 agccgattct gatgcggctc gaatccagtc tcccggaact gattcaggcc gcgctcgccg
     1261 gcgatctggt cggccggaaa atctcctggg actcgcgcag cgccgtcggg gtcgtactcg
     1321 cggccgcagg ttatccggga agcattcgcc gcggcgaccc gatccgcgga ctcgacggcg
     1381 tatttccgca ggacgtgaaa gtgtttcacg ccggcaccgc aatgcagaac ggcgatgtag
     1441 tcacggccgg cggccgggtg ctctgcgcca ccgcgctcgg caaggacttc gaggccgccc
     1501 aagccaaggc ctacgaaacc gtagaaaaga tccgcttcga gggcatgcaa tatcgccgcg
     1561 acatcggctt tagagcggtg agtgacgaga agtgaggagt gagagcacgc attgactaac
     1621 gatgaagcgc tttcctctcc actcgctctg acatgtaggt tggggtgagg tattccgaac
     1681 cccaacagcc tcatcgttta gatcgccacg ccgccgttcg ttgggcttcg ctgcgctcag
     1741 cccaacctac caagactacg actccagcca gtccctgcct tcagcgcttc atcgagttga
     1801 agaactcggc gttggtcttg gtgtccttga gcttgtcgat gaggaattcg ctcgccgcga
     1861 gttcgtccat cggatgcagg agtttccgca gaatccagat cttctgcaat tcctcttccg
     1921 tcacgagcag ttcctcgcgg cgggtgccgg agcggttgat gttgatcgcg gggaagacgc
     1981 gcttctcggc gatgcgccga tcgagatgga tttcggaatt gccggtgccc ttgaattcct
     2041 cgtagatcac gtcatccatg cgcgagccgg tttcgacgag cgcggtcgcg agaatggtga
     2101 gcgagccgcc ctcctcgatg ttgcgcgcgg cgccgaaaaa gcgcttcggg cgctgcaggg
     2161 cgttcgcgtc cacgccgccg gtgagtacct tgccggaggc cggaaccact gtgttgtagg
     2221 cgcgcccgag ccgggtgatg gaatcgagca ggatcacgac atcgcgcttg tgctcgacca
     2281 gccgcttggc cttttcgatc accatttccg cgacctgcac atgacgggtc gccggctcgt
     2341 cgaaggtcga ggaaagcacc tcgccgcgca ccgagcgggt catctccgtg acctcttcgg
     2401 gccgctcgtc gatgagcagc acgatgagca cgcagtcggg atgattgtgg gcgatcgaat
     2461 gggcgatgtt ctggagcatc atcgtcttgc ccgccttcgg cggcgagacg atgagtccgc
     2521 gctggcccat gccgagcggc gccatcagat cgatgatgcg cgccgtgatg tcctcggtcg
     2581 agccgttgcc gagttcgagc ttgaagcgtt tcttcggaaa gagcggcgtg aggttctcga
     2641 agagaatctt ggacttcgcc gcctcggggc ggtcgtcgtt gatggtgtcc acgcgcagca
     2701 gcgcgaagta gcgctcgccc ggcttcggcg gacggattct cccggtgacc gaatcgccgg
     2761 tgcgcaggtt gaagcgccga atctggttcg gactcacgta gatgtcgtcg ggcccggcct
     2821 gaaaggagct gtctccggaa cgcaggaagc cgtagccatc ggaaaggatt tcgatgacgc
     2881 cgtccccgca gatctgctcg ccattctggg cgtggccctt gaggatcgcg aagatcatgt
     2941 cctgcttgcg caggcgcgcc gcgccctcga gcccgacggc ctcggccacc tcggaaagct
     3001 ccgtggcgct cttgcgcttg agttcggaga gattcagcgt tgggccgttg ccggcctctt
     3061 cgttttcgta ttgtgattcg tccatgtctt ccatttcgac gcccggggag agaacgttgc
     3121 cgtcggattc gcgacgattg ttgccgttgc gccgggtgtt gcggcgccgg tatgcatgcc
     3181 cgctcaaagg tttgcctcca gaaaagcgac gagctgggat ttcgacagcg ctcccacctt
     3241 ctgcgcggcg acgtcgccgc ccttgaagag catcagcgtc ggaatgccgc ggatgccgta
     3301 tttgcgcgag gtctgcgcat tctcgtcgac gttgagtttg accaccttga tgcgcgcctt
     3361 gaagtcgccc gcgacttcat cgagcaccgg cgcgatcatc ttgcagggtc cgcaccattc
     3421 ggcccagtaa tccacgagca cgggcgtctc ggatttgagt acgtccgcgt cgaaggcggc
     3481 atccgacgta tgggaaacat tctgactcac cttggtcctc cagttcgacg acaaccatcg
     3541 acggcttttc gccgccgggg aaagaacgga agctccgatg cattcgcgac ggaacggatc
     3601 ggagacggct tgtccggcag agcccggatt tgccttccaa ccggatcgtg cgcagtgcgc
     3661 gcgaagcggc gatggaaatg cgcatccggc ggcacgatcg agaatccata agcagggtgc
     3721 tgggccggac aggtaggaac ggcgggccgc atgcggcaca atagagactg ctggtgtgag
     3781 tctggttggc tcgaaacggc ccgatcactc aggccggccg cggggccgcc ctgatcccat
     3841 ccgctgccga ttgtggggcc attcgcgcgc caatgcaagc cccgcgccac acgcctacga
     3901 gaatcttccc agaatgcaga accgaaaact ccccttgcgc gtctccgacc tcgcgctcgc
     3961 gccgccgatc gcggccggcg tgcgcgacgc cggcttcgag caattgacgc cgattcaaca
     4021 acgtacgctt cccgatgcgc ttgccgggca ggatgtcg
//
LOCUS       DASUJG010000011         2321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1104556, whole genome shotgun sequence.
ACCESSION   DASUJG010000011 DASUJG010000000
VERSION     DASUJG010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104556"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..668
                     /gene="ispF"
                     /locus_tag="VFX38_00160"
     CDS             183..668
                     /gene="ispF"
                     /locus_tag="VFX38_00160"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009721410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX5313312.1"
                     /translation="MRVGQGFDVHRFADGDHVMLGGVRLAHERGIVAHSDGDVLLHAL
                     TDALLGASGLGDIGALFPDTDPAYAGADSRALVSGVMSRLHETGWRVVNADCTVLAER
                     PKIAPHVAVMRAAIAQLLDVDVSAVSVKATTAERLGFVGREEGIAALAAVLIERPESR
                     S"
     gene            665..1438
                     /locus_tag="VFX38_00165"
     CDS             665..1438
                     /locus_tag="VFX38_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5313313.1"
                     /translation="MIECGELAPGIAGLTLNRPPANALSPELLAELTSALERTARDGA
                     RAIVIAGQPGMFSAGLDVPLLLTLRREEMREFWRTFYAALHALARSPVPVLIAITGHC
                     PAGGTVLALFADCRIAAEGDFKLGLNEVQVGLPMPPLVFGAFRRLVGAQAAAGLAVSA
                     QLIPPEEALRIGLVDEIVSPDRVVTKAVDKAQTLASLPAGALAATRALARADLLALFD
                     AVTDATYAEMNEVWFGAETQGAMRALVARLAERRAAASR"
     gene            1628..1990
                     /gene="rpsF"
                     /locus_tag="VFX38_00170"
                     /pseudo
     CDS             1628..1990
                     /gene="rpsF"
                     /locus_tag="VFX38_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637496.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
     gene            2191..>2321
                     /gene="rpsR"
                     /locus_tag="VFX38_00175"
     CDS             2191..>2321
                     /gene="rpsR"
                     /locus_tag="VFX38_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009562432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX5313314.1"
                     /translation="MEERSASRFRRGRKFCRFSAEGAQEIDYKDLDTLRQYLGETGK"
BASE COUNT          377 a          723 c          818 g          403 t
ORIGIN      
        1 ggagcggtgc aggacggaga cgacaagttc gcgaaggacc gccgctaccc cgtgcagcgc
       61 gtatcgccct ggctttttct cgtgatcctc gtggttaccg tcgtcgtgat tctcgccggc
      121 gtcttccgtt tcgagccgct gtcgcgcgtg gtgaattggc tcgcggtggt tctgtgagcg
      181 cgctgcgcgt cgggcaggga ttcgatgtcc atcgtttcgc cgacggcgat catgtgatgc
      241 tcggcggagt gcgcctcgcg cacgagcgcg gaatcgtcgc gcactccgac ggcgacgtac
      301 tgctgcacgc gctcaccgac gcgctgctcg gcgcttcggg actgggcgac atcggcgcgt
      361 tgtttccgga caccgatccc gcctacgcgg gggccgacag ccgcgctctc gtgtccgggg
      421 tgatgtcgcg tctgcacgaa acagggtggc gagtcgtgaa cgccgattgc accgtgctcg
      481 cggagcgtcc gaaaatcgcg cctcatgtcg ccgtgatgcg tgcggcgatc gcgcaactgc
      541 tcgatgtcga tgtttccgcg gtgagcgtca aggcgacgac cgccgaacgc ctgggcttcg
      601 tcgggcgcga ggaaggtatc gccgcgctcg ccgccgtcct catcgaacgt ccggaatcgc
      661 ggtcgtgatc gagtgcggag aactcgcgcc cggcatcgcg gggctcactt tgaatcgtcc
      721 gccggccaat gcgctttcgc cggaactcct cgcggagctg acctccgcgc tcgagcgcac
      781 ggcgcgggac ggcgcgcgcg caatcgtgat cgcgggccag ccggggatgt tttccgccgg
      841 gctcgacgtg ccgctgcttc tgacgttgcg acgcgaggag atgcgcgaat tttggcggac
      901 cttttatgcc gccttgcatg ctctcgcgcg ctcgccggtt ccggtgctga tcgcgatcac
      961 cgggcactgc ccggcgggag gaacggtgct ggcactcttt gcggactgtc gcatcgccgc
     1021 cgaaggcgac ttcaagctcg gtttgaacga agtgcaagtg ggactgccga tgccgccgct
     1081 cgtttttgga gcgttccggc ggctggtcgg tgcgcaggcc gccgcagggc tcgcggtttc
     1141 cgcgcagctg atcccgcccg aagaagcctt gcggattggg ctcgtggacg agatcgtatc
     1201 cccggatcgg gtcgtgacga aggcggtcga taaggcgcaa acgctcgcga gtctgccggc
     1261 cggggcgctc gccgcgactc gcgcgcttgc ccgcgccgac ctgctcgcgc tcttcgatgc
     1321 ggtgacggac gcgacctacg ccgagatgaa cgaggtctgg ttcggcgccg aaacccaggg
     1381 cgcgatgcgc gcgttggtgg cgcgtctggc ggagcggcgg gcggccgcct cgcgctgaac
     1441 ttgcgcccgg ggcgcaaaac ccggataatt tgccgcagaa gttccccgtc cgagcgctcg
     1501 ccgagtcgcg ggctacttcg gttcgatcgg agctttcgca accttttcgg gagtcgtctg
     1561 aattccttct ccttctcctt gcctcaccgg cgcgcaccgg gaggcttcac agccacgagg
     1621 agtcacaatg cgtcattacg aagtcgtgtt tctggtccat ccggatcaga gcgaacaggt
     1681 atccgggacc atgggtcgct acaagagcct cgtcgaaggg gcgggcggca cggtgcaccg
     1741 gctcgaggac tgggggcgac gccagctcgc ctatccgatc cagaaactgc acaaggccca
     1801 ttacgtgctg atgaacgtcg agtgcgacca gaaggcgctc gacgaactgc tcgaagcgtt
     1861 ccgattcaac gatgcggtgc tgcggcatct ggtgatgcgc cgcgatcgcg ccttcaccga
     1921 tccgtcgccg ctcgtcaagg agcgcgaacg cgaggatgcc gagaagcagg ctcgggccgc
     1981 cgccgaagag cgggccgcgc aggcgcgcgc ggaacgcgag gaagccgagg ccgagcgcga
     2041 cgaggccggg gaaaccgcgc agcagggcga gaatccgggt gaggaaagcg agaatccggg
     2101 cgaggatggg gaagatttcg agggtttcga aggcatggac gagtccggcg attccgacga
     2161 ggatgcggac gacgacgagg agaaatgaca atggaagaac gcagcgcatc gcggtttcgc
     2221 agaggacgca aattctgccg cttttcggcc gagggcgcgc aggaaatcga ctacaaggat
     2281 ctcgataccc tgcgccagta tctcggcgag acgggcaaga t
//
LOCUS       DASUJG010000012         3135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_738057, whole genome shotgun sequence.
ACCESSION   DASUJG010000012 DASUJG010000000
VERSION     DASUJG010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738057"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..1106)
                     /locus_tag="VFX38_00180"
     CDS             complement(123..1106)
                     /locus_tag="VFX38_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5313315.1"
                     /translation="MEYRRLGQSGLKISALSFGSWVTFANQVNHKRAEEMLALAYEAG
                     VNFFDNSEVYAHGRSGEMMGAVLAGLGFDRDTWCVSSKVFWGRVRDPKPTQRGLARKH
                     VIEACHGALRRLRVEYLDLYFCHRPDPDTPVEEVVRTMNTLIAQGKILYWGTSEWSAA
                     EIRAACDCAGRLGLEGPTMEQPQYNLFHRHRVEEEYASIFRDFGIGTTTWSPLASGIL
                     SGKYSDGIPEDSRFRLEGYGWLQEMIESERGRARIEAASKIAAISRELGATPAQLAIA
                     WCLRNPKVSSVILGASKPEQLRENLGALAVYQRLDDHTANKLEALTSSLAD"
     gene            complement(1170..>3135)
                     /locus_tag="VFX38_00185"
     CDS             complement(1170..>3135)
                     /locus_tag="VFX38_00185"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEX5313316.1"
                     /translation="AGVLGVLSLILWALILVVCVKYQIFVLRAKNRGDGGIIALTALL
                     RNLMRPGPERAGGGRSRARPLRAGAWIIMMGTFGAALLYGDAMITPSITVLSAVEGLH
                     RTVPELGPLIIPIACVVLFFLFWFQHRGTERVARWFAPIMVVWFLVIGGLGLASFLRL
                     PSVIAAFNPAYAVDFFLVHKWFGFVVLGSVFLTVTGAEVLYAAVGHFGDKPIRNAWFV
                     LVFPMLVLNYLGQAAMVLRDPAAAPDLFFSLAPGFGGLIPVYLLVALATMAAVIASQA
                     VISGVFSLVSQAIALNISPRVRIVRTSRAERGQVYIPSLNWALMVACIVLVVGFGGSE
                     SLAGAYGVAISSDMLITTILLFIVMRRLWHWPRYAAVGLTLLFLCMDVPFWGGNIIKI
                     AEGGWVPLVVAAAAFVLMRVWTNNRERLIATLRARTEALAIFLDRLGRAMPQRVPGTA
                     VFLTTPGLGVPPMLNYHLRHNQVLHEQVLLLSVITTDAPTVMTADRVDVVPYGYGIYR
                     VNLYYGFAQAQPVMRSLENAAARGLLRLDPARMTFYFGRETIVPSPYGGPFLGLRRWL
                     LMQWCRWRRLPVPPLREPLRVGLRQAISERLFALLHRNAPRATDFFGIPDDFTVEFGL
                     RLHSTSLAPVASAPVADAAKTEHAPGAIMN"
BASE COUNT          651 a          995 c         1016 g          473 t
ORIGIN      
        1 cgggacccct tcctgacgga agggggaaga ttggaggagc accccttcgg cggagcgact
       61 ttgtcagtgg caggcgatat tcgcttacgc gggcacggcg gtgcttgcga gccggtcgcg
      121 gatcaatcgg cgagcgatga agtcagtgct tcgagtttat tcgcggtgtg gtcgtcgagc
      181 cgctgataga ccgccagtgc gccgagattc tcgcgcagtt gttccggttt cgaggcgccg
      241 aggattacgc tcgagacttt gggattgcgc aggcaccagg cgatcgcgag ttgcgccggc
      301 gtggcgccca gctcgcggga gatcgccgcg attttgctcg cggcttcgat gcgcgcgcga
      361 ccgcgttcgc tttcgatcat ctcctgcagc cagccgtaac cttcgagccg gaagcgcgag
      421 tcttccggaa tgccgtcgct gtacttgccg ctcaggattc cggaggcgag cggcgaccag
      481 gtcgtcgtgc cgatgccgaa gtcgcgaaag atcgacgcat attcttcttc gacccgatgc
      541 cggtgaaaaa ggttgtactg cggttgctcc atcgtcgggc cttcgaggcc gagtcgcccg
      601 gcgcagtcgc aggccgcgcg gatctccgcc gcgctccatt ccgatgttcc ccagtagaga
      661 atcttgccct gcgcgatgag cgtgttcatg gtgcgcacca cctcttccac cggagtgtcc
      721 ggatcggggc gatgacagaa atagagatcg agatactcga cgcgcaggcg ccggagcgcc
      781 ccgtgacagg cctcgatgac atgcttgcgc gcgaggccac gttgtgtcgg tttgggatcg
      841 cgcacgcgcc cccagaaaac cttcgaggac acgcaccagg tgtcgcgatc gaagccgaga
      901 ccggcgagca ccgcgcccat catctcgccg gaacggccgt gagcgtacac ctcggagttg
      961 tcgaaaaaat tcacgccggc ctcgtaggcg agcgcgagca tctcctccgc ccgtttgtgg
     1021 ttcacctggt tcgcaaaggt aacccaggaa ccgaaggaca gggcggagat tttcagtccg
     1081 ctttgcccga ggcgccgata ttccatcgcc gattctcctc tcgccgcggg cgcaccgttc
     1141 cggtgacgac agccgcagcg aacatcccat caattcatga tagcgccggg agcatgttcc
     1201 gttttcgcgg cgtcggcaac gggagcgctc gcaacgggcg caaggctcgt ggaatggagc
     1261 cgcaggccga actcgacggt aaagtcgtcg ggaatgccga agaaatcggt tgcgcgcggg
     1321 gcattgcgat gcaagagcgc aaacaaacgc tcggaaatcg cctggcgcag gcctacccgc
     1381 agcggttctc tcagcggcgg aaccggcagc ctgcgccacc ggcaccattg catgagcaac
     1441 cagcggcgca atccgaggaa cggtccgccg tagggcgagg gcacgatcgt ttcccggccg
     1501 aaatagaagg tcatgcgggc gggatcgagc cgcaacaggc cgcgcgcggc ggcgttctcc
     1561 agactgcgca tcaccggttg cgcctgcgcg aagccgtagt agagattcac ccgataaatt
     1621 ccgtagccgt agggaacgac atccacgcga tccgcggtca ttaccgtggg cgcgtcggtc
     1681 gtgatgaccg acaggagcag cacctgttcg tgcagaacct gattgtgccg cagatgatag
     1741 ttcaacatcg gcggcacgcc gagtcccgga gtcgtcagaa ataccgcggt cccgggtacg
     1801 cgctgcggca tcgcgcgccc gaggcggtca aggaatatcg cgagcgcttc ggtgcgcgcg
     1861 cgcagcgtcg cgatcagacg ttcgcgattg ttcgtccaca cccgcatgag aacgaaggca
     1921 gcggccgcga cgacgagcgg cacccagccg ccctcggcga tcttgatgat gttgccgccc
     1981 cagaagggga cgtccatgca cagaaagagc agcgtcagac cgaccgcggc atagcgcggc
     2041 cagtgccaga ggcggcgcat gacgatgaac agcaggatgg tcgtgatcag catgtcggag
     2101 ctgatcgcga ctccgtacgc gccggcgaga ctttcggaac cgccgaaacc gaccacgagc
     2161 acgatgcagg cgaccatcaa cgcccagttg agcgagggga tatacacctg cccgcgctcg
     2221 gcacgcgagg tgcgcacgat ccgcacccgc ggactgatgt tgagcgcgat ggcctgactc
     2281 acgagcgaaa acaccccgga gatcaccgcc tgcgaggcga tgaccgccgc catcgtcgcg
     2341 agcgcgacga gaaggtacac cggaatcaat ccgccaaaac ctggagccag cgaaaagaag
     2401 agatcgggag ccgccgccgg atcccgcagc accatcgccg cctggccgag ataattgagc
     2461 accagcatcg gaaacaccag cacgaaccac gcattgcgga tgggcttgtc gccgaaatga
     2521 ccgaccgccg cgtacagcac ctcggcgccc gtcacggtaa gaaagaccga accgagaacg
     2581 acgaagccga accatttgtg cacgaggaaa aagtccaccg catacgccgg attgaaagcg
     2641 gcgatgacgg agggcaggcg cagaaaggaa gcgagcccga ggccgccgat cacgagaaac
     2701 cacacgacca tgatcggcgc gaaccagcgc gcgacccgtt cggtaccgcg atgctggaac
     2761 cagaagagaa aaaacaacac gacgcaggcg atcgggatga tgagcggtcc cagttcggga
     2821 acggtgcgat gcagaccttc gacggccgac agcaccgtga tcgagggcgt gatcatcgcg
     2881 tcgccataca ggagcgccgc gccgaaggtt cccatcatga tgatccaggc gccggcgcgc
     2941 agcggccggg cacggcttcg gcccccgcct gcccgctccg gcccgggtcg catcagattc
     3001 ctgagcagcg ccgtgagcgc gatgatcccg ccgtcgccgc gatttttggc gcgcagcacg
     3061 aagatctgat acttgacgca aacgacgaga atcagggccc acagaatcag cgaaagcacc
     3121 ccgaggacgc ccgcc
//
LOCUS       DASUJG010000013         6095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1594224, whole genome shotgun sequence.
ACCESSION   DASUJG010000013 DASUJG010000000
VERSION     DASUJG010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6095
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1594224"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..415)
                     /locus_tag="VFX38_00190"
     CDS             complement(50..415)
                     /locus_tag="VFX38_00190"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5313317.1"
                     /translation="MFRPRCANRFQGLGVSNTIRAACAAWAKTLARELAADGITVNNV
                     LPGYTATDRLATLFAARAERAGKTPAEIEEATRLSVPAARFAAPEEIAEAVAFLASPA
                     AGYITGVSLPVDGGRLHGI"
     gene            complement(441..845)
                     /locus_tag="VFX38_00195"
                     /pseudo
     CDS             complement(441..845)
                     /locus_tag="VFX38_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
     gene            complement(928..1782)
                     /locus_tag="VFX38_00200"
     CDS             complement(928..1782)
                     /locus_tag="VFX38_00200"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002811020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEX5313318.1"
                     /translation="MAVFAIGDVQGCAKSLKNLLARIRFDPGDDLLWFTGDLVNRGPR
                     SLEVLRFVRGLGERAVTVLGNHDLHLVAMAAGLRPPTPGLAAVLEAEDGAELIAWLRA
                     RPLLHHDPRYGYVLVHAGLAPAWTLAAAAGYAREVETALAGDGAEALLAGMYGNEPAH
                     WSEDLEGIARLRTIINVFTRLRYVSPAGLFDFEHKGAPGTQPPPLLPWFLAPGRRNAD
                     EHILFGHWSTLGPVETPGIYPLDTGCVWGGQLTAMRLDDEGGWYSVPCRRAARPQESE
                     RKRSRSEK"
     gene            complement(1801..2181)
                     /gene="apaG"
                     /locus_tag="VFX38_00205"
     CDS             complement(1801..2181)
                     /gene="apaG"
                     /locus_tag="VFX38_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEX5313319.1"
                     /translation="MNSLRREITVNVESNFLAGESEPEESRYVFGYTVTLANTGDIGA
                     RLLTRHWIITDADGEVREVHGEGVIGEAPYLTPGAGFRYSSGAVLATPVGTMHGSYQW
                     IGDDGVPFDAPIPMFRLAVPGLLH"
     gene            complement(2341..2802)
                     /locus_tag="VFX38_00210"
     CDS             complement(2341..2802)
                     /locus_tag="VFX38_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5313320.1"
                     /translation="MAGYTHVLAAVDLGADSELLVARALAVAQRFEARLSLAHVVEYV
                     LAEPAGETLLPPPMSLEPELASGAELQLAEFAARAGAGEADRHVAVGSIPAELSRLVE
                     DLGVDLLIAGAHERHWLAFFASGTERSLLKRVACDVLVVRLPKSRDGEISS"
     gene            2907..5069
                     /locus_tag="VFX38_00215"
     CDS             2907..5069
                     /locus_tag="VFX38_00215"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEX5313321.1"
                     /translation="MTLRSPAQGLVRTVALRLRPRFAPNQILLGFALRAILSIALPLA
                     IFRLAGWAPAGLYMALGALQITLAGSGGAYRDRVLGVVLPCLLLPETYLLGTQVAALW
                     WVAAPVMFLLAFASGMLRVFGAAGAPLGLLVGATYLLGTMSTSSPSSALLHTGLFLAG
                     CLWTLVLTLAAWWLRPYVALRRDVAAAIDAAATLLAALPERAAAELVNDALEVRARIE
                     TARSALGMTQDMSAAINDTLAVLYALLHLPSHMATLALDLAQLRPRLGADARALASFD
                     AGCIALARHAEALSRALASGQGWSFEEPIAASIEALRVYRVTDDELRPRVALAVEALA
                     STASRLREAARLTQELARSVRRPAADWLPQIGLGMRLRRSVRAIGTQLRWRSAIFRYA
                     LRIAFVAALAVAVEVSLRVPHGIWLPLTVLVIMQPDFGATLPRAFARAGGTLAGVVIA
                     GAILVAVPWPAGREAAVFVLAFLMALTLRLRYGFFVACLTPLVVLLLALYQPGEGWNF
                     VVERVLETAAGTVLAILGALLLWPAKARLRLPGEFSLALRAAGAYLLAAFSAAASGER
                     RLQPVYDARRTAELAVSNAQAALHTTRTEPWRKPGERRHYLELELRLKRLIRLLDILT
                     VRIEIDHPSLGALHPLATRWVDALRFMADSLQTAAAPPAGHEKFAAELPVLPESLAKP
                     IRWIGETIAEIGEATRAGPAGGPAAPEHAPEAALPAPH"
     gene            complement(5147..5974)
                     /gene="rsmA"
                     /locus_tag="VFX38_00220"
     CDS             complement(5147..5974)
                     /gene="rsmA"
                     /locus_tag="VFX38_00220"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX5313322.1"
                     /translation="MYANKRLGQHFLHDPAVIARILAAIAARPGEHLVEIGPGRGALT
                     RPLAASGARLTVIELDRGLAAALAADPALAAVEVIVADALRTDYTALAGGAPLRIVGN
                     LPYNISSPLLFRLLEAAARIRDLTLMLQREVVERMVAAPATRAYGRLTVMLAARCRVE
                     RLFVVGAGAFVPAPKVESAVVRLVPHLVPPFDWGDWELFEQLVRAGFSARRKTLRNAL
                     QAFADAKTIEAAGLDAGARPGTLAAADYARLSCLVASRGVACDFFGDHANHEVSGKE"
BASE COUNT          961 a         2100 c         2047 g          987 t
ORIGIN      
        1 ttgtaggctc gcgacggcga agcgttgcgg gcgctacgcc gatgcccgct cagatgccgt
       61 gcaggcgccc gccatccacg ggaagcgaaa cgccggtgat gtagccggct gccggcgatg
      121 cgagaaaggc cacggcctcc gcgatctctt ccggggcggc gaagcgcgca gccgggacgg
      181 acaaacgggt ggcctcctcg atttcggcgg gagtcttgcc ggcgcgttcg gcgcgggcgg
      241 caaagagcgt tgcaagccga tcggtcgccg tgtaaccggg aagcacgttg ttgacggtga
      301 tcccgtcggc ggcgagttcg cgcgcgagcg ttttcgccca ggccgcgcag gccgcgcgaa
      361 tcgtgttcga aacgccgagc ccctggaatc ggttcgcgca ccgaggtcga aacaatattg
      421 acgatgcgcc cgtaccctgc ctcgcgcatc cccggcacca gcgtctgcgc gagaacctgt
      481 gcggcgatca gcaggcgttc gaacgccctg cggaattgct cgggcttcgc atcgaggatc
      541 gcaccgccgg gcggaccgcc ggagttgttg acgaggatgt gaattgggcg cggacttctt
      601 tcgagcagtg acgccagcgc caccgtgtcg tcgagatcgg cgacgagcgt gcggtgctcc
      661 gcggcgccga cgacgggaag cgcggcacgc agattctcca gaccttcgcc gtcgcgcgcg
      721 aggagtgtca ggctcgcacc gcgggcggcg agaagttccg ccgtcgcccg cccgatgcca
      781 cggctcgctc cgcacaccaa cgcccgatag cccgcgagat ttcgatccgg cacgagtata
      841 gtcacaacaa attcttcctc atcttacgca gaagtaagaa gggaggcgtg cgtaatgaga
      901 aagcgtgcga aaccaggttt tccattctca cttctcgctt ctcgaccgtt tcctctcgct
      961 ttcctgcggg cgcgccgcgc ggcggcaggg aaccgagtac caacctcctt cgtcgtcgag
     1021 ccgcatggcc gtcaactggc cgccccagac gcatcccgtg tcgagcggat agattccggg
     1081 agtttccacc ggcccgagcg tcgaccagtg gccgaagagg atgtgctcgt cggcattgcg
     1141 ccgccccggc gcgaggaacc acggcagcaa cggcggcggc tgcgttcccg gcgcgccctt
     1201 gtgctcgaag tcgaagagcc ctgccggcga aacataacga aggcgcgtga acacgttgat
     1261 gattgtgcgc agccgggcaa tgccttcgag atcctcggac cagtgcgcgg gctcgttccc
     1321 atacatcccg gcgaggagcg cctcggcgcc gtcgccggcg agcgcggtct cgacttcgcg
     1381 ggcatatcct gcggccgcag cgagcgtcca ggccggggcg agtccggcat gcacgagcac
     1441 atagccgtag cgtggatcgt gatgcaagag cgggcgcgcc cgcagccagg cgatgagttc
     1501 cgctccgtcc tcggcttcga gcacggcggc gagcccgggg gtcggcggcc gcagtcccgc
     1561 cgccatcgcg acgagatgca gatcgtgatt gccgagcacg gtgacggcgc gctcgccgag
     1621 cccgcgcacg aagcgcaaca cctcgagcga gcgggggccg cggttcacga gatcaccggt
     1681 gaaccacaaa aggtcgtcgc ccggatcgaa gcggatgcgg gcgagaaggt tcttcagcga
     1741 cttggcgcag ccctggacgt caccgatggc aaaaacggcc atgccggttc ggcatgcggc
     1801 tcagtgcaac aaaccgggga cggcgaggcg gaacatgggg atcggcgcat cgaagggaac
     1861 cccgtcgtcg ccgatccact gatagctccc gtgcatggtt ccgacgggcg tcgcgagcac
     1921 cgcgccggag ctgtagcgaa agccggcgcc cggagtgagg tagggcgcct cgccgatgac
     1981 cccctcgccg tgcacctcgc gcacttcccc gtcggcatcg gtgatgatcc agtggcgggt
     2041 cagcaggcgc gcgccgatgt cgccggtgtt ggcgagagtc acggtgtagc cgaacacgta
     2101 gcggctctcc tcgggttccg attcgcccgc gaggaaattg ctctcgacat tgaccgtgat
     2161 ttcacgccgc aaggaattca cctgctctct cccgccggat gcgctgcgat tctaccagtg
     2221 ccgggtatcg gtcggcactc cgccccgcga aaccgcgatc cgccgccggg gagggagccg
     2281 cgcagaaacc ttgccggagc tgctgcggca attcgatgac gaagttctcc gcgagcctgc
     2341 tcaggatgag atctcgccgt cgcgagactt gggcagccgt acgaccagca cgtcgcaggc
     2401 cacccgcttg agcagcgaac gctcggtgcc gctcgcgaaa aaagcgagcc agtgccgttc
     2461 gtgcgcgccg gcgatgagaa gatccacgcc gagatcctcg acgaggcgcg aaagctccgc
     2521 tgggatgctc ccgaccgcga cgtgacgatc ggcctcgccc gctccggcgc gcgcggcgaa
     2581 ctccgcgagc tgcagttccg cgccgctcgc gagttcgggc tcaagcgaca tcggcggcgg
     2641 gagcagcgtt tcgccggccg gttcggcgag aacgtattcg accacgtggg cgaggctcaa
     2701 gcgtgcctcg aagcgctgcg cgaccgcaag cgcgcgagcg acaagcaatt ccgaatcggc
     2761 tccgagatcg acggcggcga gcacgtgcgt atagccggcc attgcgaact ccttcgctcg
     2821 aggcggcgcc gcctctatga tgtgtcatcc tgcggcgcag ggaagtattt gtcaatgggc
     2881 tcatgccggc gcgagaggtc cgagacgtga cgctgcgatc cccggcgcag ggcctcgtgc
     2941 gcaccgttgc gctgcgcctt cgcccgcgat ttgcgccgaa ccagatcctc ctcggtttcg
     3001 cgctgcgcgc catcctttcg atcgccctgc cgctcgcgat attccggctc gcgggatggg
     3061 cgccggccgg gctgtacatg gccctggggg cgcttcagat cacgctcgcg ggaagcggag
     3121 gagcctaccg cgatcgtgtc ctcggcgtgg tgctcccctg tctccttctg cccgagactt
     3181 acctgctcgg cacgcaggtc gccgcgctct ggtgggtcgc ggcgcccgtg atgttcctgc
     3241 tcgcttttgc ctccggaatg ctgcgcgtgt tcggagccgc gggcgcgccg ctcggactgc
     3301 tcgtgggcgc gacctatctg ctcggaacca tgtccacctc ctcgccttcg agcgcgctcc
     3361 tgcacacggg gctgttcctc gccggttgcc tgtggacgct cgtgcttacg ctcgccgcct
     3421 ggtggcttcg gccctatgtc gcgctgcgtc gcgatgtcgc ggccgcgatc gacgccgcgg
     3481 caacgctgct cgcggcgctg cccgaacgcg ccgccgcgga actcgtcaac gatgcgctcg
     3541 aagttcgcgc caggatcgag accgcgcgca gcgcgctcgg gatgacgcag gacatgagcg
     3601 cggcgatcaa cgacacgctc gcagtcctgt acgcactcct gcacctgccc tcgcacatgg
     3661 caaccctcgc tctcgatctc gcgcaactgc ggccgcggct cggcgccgat gctcgcgcac
     3721 tcgcgtcctt cgatgccggc tgcatcgcac ttgcgcgcca cgccgaggcg ctttcccgcg
     3781 cgctcgcctc cgggcagggc tggagtttcg aagaaccgat cgcggcttcg atcgaggcgc
     3841 tgcgcgtata tcgcgtcacc gacgacgagc tgcgcccgcg tgtcgcgctc gcggtagagg
     3901 cgctcgcgag taccgcgtcg cgtctgcgcg aagccgccag actcacgcag gaactcgcgc
     3961 gcagcgtccg ccggcccgcc gcggattggc tcccgcaaat cgggctcggc atgcggctgc
     4021 gccgatccgt ccgcgccatc gggacacaat tacgttggcg ctcggcaatt ttccgctacg
     4081 ccctgcgcat cgccttcgtt gccgcgctcg ccgtcgcggt cgaagtgtcc ttgcgcgttc
     4141 cgcacggaat ctggctgccg ctgaccgtgc tcgtgatcat gcaacccgat ttcggcgcga
     4201 cgctgccgcg cgcgttcgcc cgcgccggcg ggacgctcgc cggcgtcgtc atcgccggcg
     4261 cgattctggt tgcggtgccg tggccggcgg gtcgggaagc cgcggtcttc gtcctcgcat
     4321 ttctgatggc cttgacgctg cgtctgcgct acggattctt cgtcgcctgt ctcactccgc
     4381 tcgtcgtgct gcttcttgcg ctctatcagc cgggcgaggg ttggaatttc gtcgtcgaac
     4441 gggtgctcga aaccgcggcg ggaaccgtgc tcgccatcct gggagccttg ctcctctggc
     4501 cggcgaaggc aaggctgcgc ctgcccggag aattttccct cgccctgcga gctgcaggcg
     4561 cttatctcct tgcggccttc tccgccgccg cgagcggcga gcgcaggctg caacccgtgt
     4621 acgacgcccg ccgcacggcg gaactcgccg tgagcaatgc gcaggcggcc cttcacacta
     4681 cgcgcaccga accctggcgc aagcccggcg agcgccgcca ctacctcgaa ctggaactgc
     4741 gtctgaaacg cctgatccgt cttctcgaca tcctcacggt gcgcatcgaa atcgatcatc
     4801 cttcccttgg tgcgctgcat ccgctcgcga cacgctgggt cgacgcgctt cgcttcatgg
     4861 ccgattcgct gcaaacagcg gccgcgcctc ccgccgggca cgagaaattc gcggcagagc
     4921 ttccggtcct tccggagagc ctcgccaaac cgatccgctg gatcggcgag acgatcgccg
     4981 agatcggcga ggcgacgcgc gccggtccgg ctggcggccc ggccgccccc gagcatgccc
     5041 ccgaagccgc tctgcctgcc ccgcactgaa acgccgtggg cgatcgtgtc aaggcgaaaa
     5101 atctcgtgcg gcacgaaatg ttcgcgctcg cgccgtatgg tcgtcgctat tccttgccgc
     5161 tcacctcatg attcgcgtga tcgccgaaaa aatcacaagc cacgccgcgg cttgcgacca
     5221 agcaggataa gcgggcgtag tcggcggcgg cgagcgtccc ggggcgagcg cccgcatcga
     5281 ggccggcggc ctcgatggtc ttcgcgtcgg cgaatgcctg caaggcgttg cgcagggtct
     5341 tgcgccgtgc cgagaatccc gcgcgtacga gttgctcgaa caactcccaa tcgccccaat
     5401 cgaagggcgg aacgagatgc gggacgagac gcacgacggc ggattcgacc ttcggcgcgg
     5461 gaacgaaggc gccagcgccg acgacaaaca gcctctccac gcgacaccgc gccgcgagca
     5521 tgacggtcag ccggccgtag gcgcgcgttg cgggcgcggc aaccatgcgt tcgaccactt
     5581 cgcgttgcag catcagggtg aggtcgcgaa tgcgggcggc cgcctcgaga aggcgaaaaa
     5641 gcagcggcga ggaaatgttg tagggcagat tgccgacgat gcgcaaaggc gccccccccg
     5701 cgagcgccgt gtagtcggtc cggagcgcat cggcgacgat gacttcgacc gcggcgagcg
     5761 cgggatcggc cgcgagtgcc gccgcgagac cccgatcgag ttcaatcacg gtgagccgcg
     5821 caccgctcgc ggccagaggc cgggtaagcg ccccgcgtcc cggtccgatt tcgacgaggt
     5881 gctcgcccgg gcgggctgcg atcgcggcga ggatgcgcgc gatcaccgcg ggatcatgca
     5941 gaaaatgctg gccgagacgt ttgtttgcgt acacgctgga agactaaacg caagttccac
     6001 aaaagtcgca gcatacgcga gcgggatgcg cgatcaccgg acttttgttg acggcgcgcc
     6061 gggcctctgc gaacgggatc aaccgggcgt gctct
//
LOCUS       DASUJG010000014         5601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_615496, whole genome shotgun sequence.
ACCESSION   DASUJG010000014 DASUJG010000000
VERSION     DASUJG010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615496"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VFX38_00225"
     CDS             <1..702
                     /locus_tag="VFX38_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metalloprotease"
                     /protein_id="HEX5313323.1"
                     /translation="EEKRRTAYHEAGHAVVGLSVPGHDPVYKVTIIPRGRALGVTLFL
                     PETDRYSASRQQLESQLASLFGGRLAEEIVFGKEQVTTGASNDIERATQIARNMVTKW
                     GLSERLGPLSYSEEDDEVFLGHSVTRHKQVSDDTAHAIDEEIRSIIDRTYHFAFDILT
                     ANRGKLDNMAEALVKYETIDAEQIADIMAGREPRPPKDWTDPGGPGDEARGRAPAGKT
                     GRETGEPIGGPASQH"
     gene            888..1673
                     /gene="folP"
                     /locus_tag="VFX38_00230"
     CDS             888..1673
                     /gene="folP"
                     /locus_tag="VFX38_00230"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX5313324.1"
                     /translation="MGVINVTPDSFSDGGKFLDPGAAADQAARLVAEGAAILDVGAVS
                     TRPGAQAVAPEEEQRRLLPALERIVAESKTPVSVDTSNAAIMRLAVEAGAAMINDQRA
                     LREPGALEAAAAADCAVCLMHMQGSPETMQYAPRYADVVKEVCTFLETRADACVDAGI
                     APDHILLDPGFGFGKSVEHNLRLLRHLPEFLDPGYPLLVGLSRKSSIGKITGAGPDER
                     LAGSLAAATIAVWLGASIVRAHDVKATVEALAFTDAVRRSTTS"
     gene            1685..3031
                     /gene="glmM"
                     /locus_tag="VFX38_00235"
     CDS             1685..3031
                     /gene="glmM"
                     /locus_tag="VFX38_00235"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF008139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX5313325.1"
                     /translation="MRRWFGTDGIRGRVGIAPLTPEFVLHLGWATARVLGAAGGRIAV
                     VGKDTRRSGYMFESALEAGFSAAGMDVLLLGPIPTPAIAHLTRSVGAAVGVVISASHN
                     PYGDNGIKFFGPDGRKLADATEADIENALAEPMEVVESRHLGGARRMSDARERYVEFC
                     KASLPPQTSFAGMRLVLDCAHGATYAVAPRVFRDLGAEVIVIAADPDGLNINDGCGSM
                     HPAQMIAAVREHGADYGFAFDGDGDRVLAADASGRLYDGDDLLYVMARERAARGMLTG
                     SVVGTVMSNLGLEQALAAAGIGFERVAVGDRHVLARLDQIGGVLGGETSGHIICLDRA
                     SSGDGIIAALEVLAAVRERGRGLAELLAPLVKRPQLLRNVPLGAASPEALMQDDDLAR
                     VRATVEARLGVQGRVLLRPSGTEPLLRIMVEGPELALVEDCVAALVAVVEERLALL"
     gene            3083..3880
                     /gene="tpiA"
                     /locus_tag="VFX38_00240"
     CDS             3083..3880
                     /gene="tpiA"
                     /locus_tag="VFX38_00240"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX5313326.1"
                     /translation="MRRALVAANWKMHGRAALIGGYVSQLARAAESARDDVEVLLCPP
                     TPYLGAAAAATADTDIALGAQDCSYEEEDGAFTGEASAAMLADAGCGWVLVGHSERRR
                     RWAESDALVAAKFRAVQGAGLKPILCVGETEGERADGRAESRVLAQLAAVMERCEMDQ
                     LGRAALAYEPVWAIGSGQAASPADAQSLHAALRAAVAERDRAAARRIRILYGGSVSGA
                     NAGEFFAQPDIDGALVGGASLDAGTFSGIVAAAREVRHRQEQSRGAA"
     gene            3935..4345
                     /gene="secG"
                     /locus_tag="VFX38_00245"
     CDS             3935..4345
                     /gene="secG"
                     /locus_tag="VFX38_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX5313327.1"
                     /translation="MLFSILIAVMVVLGIVLIALILVQQGKGADAGAAFGSGASGTVF
                     GSRGSATFLSRATGVLMALFMALAIVMVYIGHRAPAQTGSVMASAPAAAATFAPPAKA
                     ASAPVAPAATAGAKARSAAPAGSAPNPATGAGRA"
     gene            4399..4484
                     /locus_tag="VFX38_00250"
     tRNA            4399..4484
                     /locus_tag="VFX38_00250"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4434..4436,aa:Leu,seq:gag)
     gene            4724..5080
                     /locus_tag="VFX38_00255"
     CDS             4724..5080
                     /locus_tag="VFX38_00255"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012982121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX5313328.1"
                     /translation="MLQNYLSVLIFIVVGGGLGALLIIIGLLVAPHRPDEEKNSPYEC
                     GFEAFEDTRMRFDVRYYLVALLFIIFDLEIAFLFPWAVALETIGLFGLFAMAVFLAVL
                     IVGFIYEWKRGALEWE"
     gene            5071..5565
                     /locus_tag="VFX38_00260"
     CDS             5071..5565
                     /locus_tag="VFX38_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014703781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX5313329.1"
                     /translation="MGIEGTLPAGVLTTTVDKIVNWARTGSLWPMTFGLACCAVEMMH
                     AGAARYDLDRFGVIFRPSPRQSDVMIVAGTLVNKMAPALRKVYDQMPDPKWVISMGSC
                     ANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALLFGILQLQQKIRRTNTIARTH
                     TIAR"
BASE COUNT          871 a         1816 c         1958 g          956 t
ORIGIN      
        1 gaggagaagc gccgcaccgc ctatcacgag gcggggcacg cggtcgtcgg gctttccgtt
       61 cccggacacg accccgttta taaggtgacg atcattccgc gcggtcgcgc gctcggcgtg
      121 acgctcttcc tgcccgagac cgatcgctac agcgcgagca ggcagcagct cgagagccag
      181 ctcgcgagcc tcttcggcgg gcgcctcgcc gaggagatcg tgttcggcaa ggaacaggtg
      241 acgaccggtg cttcgaacga catcgagcgc gccacccaga tcgcgcgcaa catggtgacc
      301 aagtgggggc tttccgagcg cctcggtccg ctcagctaca gcgaggaaga cgacgaggtg
      361 tttctcgggc attcggttac ccgccacaag caggtttccg acgacaccgc ccacgcgatc
      421 gacgaggaaa tccgctcgat catcgatcgc acctaccatt tcgccttcga tatcctcacc
      481 gccaaccgtg gcaagctcga caacatggcc gaggcgctcg tcaaatacga aaccatcgat
      541 gccgagcaga ttgcggacat catggcgggt cgcgagccgc gtccgcccaa ggattggacc
      601 gatccgggcg gcccgggcga cgaggcgagg ggacgggcac cggcgggcaa aaccgggcgc
      661 gaaaccggag agccgatcgg cgggccggca agccagcatt aacgcggcgg cgcgtcccgg
      721 gactcgcatc ccggaagagt aaacttgatc gcttccggtg tagacatggc gttgcggtct
      781 ttaccgcggg acaattcccg atgaccttgc gcggcgcggc cagtccgtcg caggcgccgg
      841 tgctgcgatg gtacggcggc gcgctcgatc tcggcaggcc acgggtgatg ggcgtgatca
      901 acgtgacgcc cgattcgttt tccgacggcg ggaagtttct cgatcccggc gcggcggccg
      961 atcaggccgc gcgcctggtc gcggagggcg cggcgattct cgacgtcggc gccgtttcca
     1021 cgcgaccggg tgcgcaggcg gttgcaccgg aggaggagca acggcggttg ctcccggcgc
     1081 tggagcgaat cgtcgccgaa agcaaaaccc ccgtttccgt cgatacgagc aatgcggcga
     1141 tcatgcgact cgccgtcgag gcgggagcgg cgatgatcaa cgaccagcgc gccctgcgcg
     1201 agcccggagc gctcgaggcg gcggcggccg ccgactgtgc ggtctgtctc atgcatatgc
     1261 agggcagccc ggaaaccatg cagtacgctc cccgttacgc ggacgtggtg aaggaggtct
     1321 gcacgttcct cgagacgcgt gccgacgcct gcgtcgacgc ggggatcgct cccgatcaca
     1381 ttctgctcga tcccgggttt ggcttcggca agagcgtcga gcacaatctg cggctgctgc
     1441 ggcatcttcc ggagtttctc gaccccggct atccgctgct cgtcggcctg tcgcgcaaat
     1501 cctcgatcgg aaagattacc ggagcgggcc ccgacgagcg cctggccggc tcgctcgccg
     1561 cggcaacgat cgcggtgtgg ctgggcgcct ccatcgtgcg cgcacatgac gtgaaggcca
     1621 cggtcgaggc gctcgccttc accgacgccg tgcgccgctc gacgacctca tgaacgcgcc
     1681 ggcgatgcga cgctggtttg gaaccgacgg catccgcggt cgcgtcggca tcgcgccgct
     1741 cacaccggaa ttcgtgctgc atctcggctg ggccaccgcc cgcgtgctcg gcgccgccgg
     1801 cgggcggatc gcggtcgtcg gcaaggacac gcgccgctcc ggctacatgt tcgagtccgc
     1861 gctcgaggcg ggcttttccg ccgccggaat ggacgtgctt cttctcgggc cgattccgac
     1921 gcccgcgatc gcccatctca cgcgcagcgt cggcgcggcg gtcggggtgg tcatcagcgc
     1981 ctcgcacaat ccctacggcg acaacggcat caaatttttc ggcccggacg ggcgcaagct
     2041 cgccgatgcg accgaagccg atatcgagaa cgcgctcgct gagccgatgg aggtggtcga
     2101 gtcgcggcat ctgggtggcg cccggcgcat gagcgacgcg cgggaacgct acgtcgaatt
     2161 ctgcaaggct tcgctgccgc cgcagacttc cttcgcgggc atgcgcctcg tgctcgattg
     2221 cgcgcacgga gccacctatg ccgtcgcccc gcgggtattc cgcgatctcg gtgccgaggt
     2281 catcgtgatc gccgccgatc ccgacggcct caacatcaac gacggctgcg gttcgatgca
     2341 tccggcgcag atgattgcgg cggtgcgcga gcacggtgcc gactacggtt tcgccttcga
     2401 cggcgacggc gatcgggtgc tcgcggcgga cgcctccggg cgcctgtacg acggcgacga
     2461 tcttctctac gtcatggctc gcgaacgagc ggcccgcgga atgctcacgg gctcggtggt
     2521 cggcacggtg atgagcaatc tcgggctcga gcaagccctg gctgcggccg gaatcggttt
     2581 tgagcgcgtc gccgtcggcg accgccacgt gctcgcgcgc ctggaccaaa tcggcggagt
     2641 tctcggcggc gagacttcgg gccacatcat ctgtctcgat cgggcgagca gcggcgatgg
     2701 catcattgcg gcactcgagg tgttggcggc ggtgcgcgag cgcggccgcg ggctcgccga
     2761 gttactcgcg cctctcgtga aacgcccgca gttgctgcgc aacgtgccgc tgggggccgc
     2821 gtccccggag gcgctgatgc aagacgatga ccttgcgcga gtgcgagcca ccgtcgaggc
     2881 gcggctcggc gttcagggga gggttcttct gcgtccatcg ggaacggagc cgctgctccg
     2941 gatcatggtg gagggacccg aactcgcgct ggtcgaggat tgcgtggctg cactcgtggc
     3001 ggtggtggag gaacggctcg cattgctttg attccgatcg gccgctaaaa tagccagctt
     3061 tcccttgcgc ggaccgcagc cgatgcgtcg ggcgctcgtc gcagccaact ggaagatgca
     3121 cggccgcgcg gcgctgatcg gcggctacgt gagccagctc gcccgcgcgg cggagagcgc
     3181 tcgcgacgac gtggaagtcc tgctctgtcc tcccacgccc tatctcggcg ccgccgccgc
     3241 ggcaacggcg gacaccgata tcgcactggg agcgcaggac tgctcctacg aagaggagga
     3301 cggcgcgttc acgggcgaag catcggcggc gatgctcgcc gacgcgggct gcggctgggt
     3361 gctggtcgga cattccgagc gccgccggcg ctgggcggag agcgacgcgc tggtggcggc
     3421 gaagtttcgt gcggtgcagg gcgcgggctt gaaaccgatt ctctgcgtcg gcgagacgga
     3481 aggcgagcgg gcggacggcc gggccgagtc ccgggtgctc gcgcagctcg cggcggtgat
     3541 ggagcgttgc gaaatggatc agctgggacg ggcggcgctc gcctacgaac cggtgtgggc
     3601 gatcggatcg gggcaggccg caagtcccgc ggatgcccag agtttgcacg cggcgctgcg
     3661 cgcggccgtc gccgagcgcg accgcgctgc ggcgcggcgg attcgaatcc tgtacggcgg
     3721 gagcgtgagc ggcgccaatg ccggggagtt ttttgcgcag ccggacatcg acggagcgct
     3781 ggtcggtggc gcctcgctcg acgccggaac cttctccgga atcgtcgcgg cggcgcgcga
     3841 agtccggcat cgacaggaac agagccgggg tgccgcgtag cggctgccgg cgcaagaggg
     3901 cccacgcggc tcgctcacgg agtacggaaa ggaaatgctt ttttcgatat tgatcgcggt
     3961 catggtggtg ctcggcatcg ttctcatcgc gctgattctg gtgcagcagg gcaagggagc
     4021 ggatgcgggc gcggccttcg gcagcggtgc ttccggaacc gtattcggat cgcggggctc
     4081 ggcgaccttt ctcagtcgcg cgaccggtgt cttgatggcc ttgttcatgg cgctcgccat
     4141 cgtgatggtg tacatcgggc atcgtgctcc cgcgcagacg gggagcgtca tggcatcggc
     4201 accggccgca gccgcgacgt ttgcgccacc ggcgaaggct gcgagcgcgc ctgtcgcacc
     4261 ggccgctacg gcgggcgcga aagcccgtag cgcggcgccg gccgggtcgg ctcccaatcc
     4321 ggccaccgga gcggggcgcg cctgaagcac cgacccggca atggcggggc gacccgctag
     4381 aatgtcggct ctggattcgc ggatgtggtg gaatctggta gacacgctgt cttgaggggg
     4441 cagtggcgca agccgtgccg gttcgagtcc ggccatccgc accactacag acgacgggaa
     4501 gttgctgcgc ccggcggatt gggcgcaggc gatacccggc atccgcacgt agcggtatgt
     4561 gcacggcggc gtctgcgtcg tggcgacgaa tccgaatccg cgaggtcgcg gatcgaatgg
     4621 cgatcttgcg atcggcaacc cggtcgatcg gggcctcgcg acgatcggtt acggtaaact
     4681 agcccgattc agcgggtcga tctcaccgga aatgatgacg ggcatgctgc agaactatct
     4741 ttccgtattg attttcatcg tcgtcggcgg gggactcggc gctcttctca tcatcatcgg
     4801 tctgctcgtc gccccccatc gtcccgacga ggaaaagaat tccccctacg agtgcggctt
     4861 cgaagccttc gaggacaccc gcatgcgttt tgacgtgcgc tactacctcg tggcgctgct
     4921 cttcatcatc ttcgatctcg agatcgcctt tctcttcccc tgggcggtgg cactcgagac
     4981 gatcgggctc ttcggcctgt tcgccatggc cgtgtttctc gcggtgctca tcgtcggctt
     5041 catctacgag tggaaacgcg gagcgctcga atgggaatag aggggacgct ccccgccggc
     5101 gttctgacga cgacggtgga caagatcgtc aattgggcgc gaaccggctc gctgtggccg
     5161 atgaccttcg gtctcgcctg ctgtgcggtc gagatgatgc atgccggggc ggcgcgctat
     5221 gacctcgatc gcttcggtgt catcttccgg ccgagtccgc gccagtccga tgtcatgatc
     5281 gtcgccggga cgctggtcaa caagatggca ccggcgctcc gcaaggtcta tgaccagatg
     5341 cccgatccca agtgggtcat ttcgatgggc tcctgcgcga acggcggcgg ctactaccat
     5401 tattcgtatt cggtggtccg cggctgcgat cgcattgtgc ccgtcgacgt ttacgtgccg
     5461 ggctgcccgc ccacggccga ggccttgctg ttcggcattt tgcagctgca gcaaaagatc
     5521 cgccgcacga acacgatcgc ccgcacgcat accatcgcgc gttgaaactc gtgtcctcgt
     5581 gccccctggc tcatcaatag a
//
LOCUS       DASUJG010000015         2541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_983829, whole genome shotgun sequence.
ACCESSION   DASUJG010000015 DASUJG010000000
VERSION     DASUJG010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983829"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..110
                     /locus_tag="VFX38_00265"
     CDS             <1..110
                     /locus_tag="VFX38_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal L9 C-terminal domain-containing
                     protein"
                     /protein_id="HEX5313330.1"
                     /translation="MPASGPIHELGEFAVALHLHSDVNATVTIAVVAEE"
     gene            130..1521
                     /gene="dnaB"
                     /locus_tag="VFX38_00270"
     CDS             130..1521
                     /gene="dnaB"
                     /locus_tag="VFX38_00270"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX5313331.1"
                     /translation="MATAPQEDDAAVGRLKVPPHSLEAERAVLGGLMLDNSTWEQIGD
                     RLRADDFYRRDHQLIFDAIGDHAERGAPFDVVTVSDWLGARERLEPAGGMSYLIGLAQ
                     DTPAAVNVRAYADIVAQRAMLRALISAGGEIAGSAYATDGRSLAEIIDEAERRVFEIA
                     ERGERRNRGFRKINELLSVTVDRLEELQNSKSHITGLPTGLADLDERTAGLQAGELII
                     IAGRPSMGKTALAMNIAENVAISEKRATAIFSLEMPAVQLTMRLVSSLGRIDQSRLRT
                     GQLEDSEWPRVSSVIQMMSDAPLYVDDSGALSPTELRTRARRLKRDVGDLSMIIVDYL
                     QLMQVPGTRENRATEISEISRSLKALAKELSIPIVALSQLNRGVEQRPNKRPVMSDLR
                     ESGSIEQDADLIMMLYRDEYYNESSPEKGLAEILIAKQRNGPTGTVRAAFLERYTRFD
                     NFAPDSALPENFR"
     gene            1521..>2541
                     /gene="alr"
                     /locus_tag="VFX38_00275"
     CDS             1521..>2541
                     /gene="alr"
                     /locus_tag="VFX38_00275"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX5313332.1"
                     /translation="MRVRARIDREALANNLALLRKLAPASRILAVVKADGYGHGLIHA
                     AQALATADGFGVTSVEEALELRESGFRGTVVVLEGALAGKELKAAAAARLDLVVHADW
                     QLDMLERDGSPANARFWVKFDTGMHRLGFPTATADRVRTRLDAIGAANTVLMSHLACA
                     DEPSRPETERQLQAFDRLRGFFPGAASLANSAGLLAHPATHCDWVRPGLALYGVSPVA
                     GRAAGEFGLRPAMTLTSRILSIREVAAGEAVGYGAAWRATRASRIGIVSIGYGDGYPW
                     RSISTGFALVAGRRIPLAGRVSMDMLGLDLTELPDVGPGDVVTLWGEGLPVEEVARAA
                     GTTPYE"
BASE COUNT          438 a          850 c          846 g          407 t
ORIGIN      
        1 gcatgcccgc gagtgggccg attcacgagc tgggtgaatt tgccgtcgcg ctgcatctgc
       61 acagcgacgt gaacgccacc gtgaccatcg cggtggtcgc cgaggaataa cgcgggcggg
      121 gacgcgatca tggccaccgc cccgcaggaa gacgacgctg ccgtagggcg gctcaaggtt
      181 ccgccgcact cgctcgaggc ggagcgcgcc gtgctcggcg ggctcatgct cgacaattcg
      241 acctgggagc agatcggcga ccgcctgcgc gctgacgatt tctatcgccg cgatcatcag
      301 ttgatattcg acgcgatcgg cgatcacgcc gaacgtggcg ctcccttcga tgtcgtcacc
      361 gtgagcgact ggctcggagc ccgcgagcgc ctggaacccg ccggcggcat gagttatctc
      421 atcggcctcg cgcaggacac gccggccgcc gtgaacgtgc gcgcctacgc cgacatcgtc
      481 gcgcaacggg cgatgctccg cgcgctcatc agtgccggcg gcgaaatcgc gggttcggcc
      541 tacgcgaccg acggccgttc tctcgccgag atcatcgacg aggccgagcg gcgggtgttc
      601 gaaatcgccg aacgcggcga gcggcgcaat cggggtttcc ggaagatcaa tgaactcctg
      661 agcgtcaccg tggaccggct ggaggaactg caaaactcca agagccacat cacgggcctg
      721 ccgacgggac tcgccgatct cgacgagcgc accgcggggc tgcaagcggg cgagctgatc
      781 atcatcgccg gccgcccatc gatggggaaa accgcgctcg cgatgaatat cgccgagaac
      841 gtcgcgatca gcgaaaagcg cgcgaccgcg atcttcagcc tcgaaatgcc cgcggtgcag
      901 ctcaccatgc gtctcgtttc ttcgctcggg cgcatcgacc agagccggct gcgcacggga
      961 cagctcgagg attcggaatg gccgcgcgtg agttccgtga tccagatgat gagcgatgcg
     1021 ccgctctacg tggacgattc cggcgcgctt tcgccgacgg aactgcgcac gcgcgcgcgc
     1081 cggctcaagc gcgacgtcgg cgatctttcg atgatcatcg tcgactatct gcaactcatg
     1141 caggtgccgg gaacgcgcga gaatcgcgcc accgagatct cggagatttc gcgttccctc
     1201 aaggcgctcg cgaaggagct ttcgattccg atcgtcgcgc tctcgcagct caaccgcggc
     1261 gtcgagcaac gcccgaacaa gcgtcccgtc atgagcgatc tgcgcgaaag tggatcgatc
     1321 gagcaggatg cggatctgat catgatgctg tatcgcgacg agtactacaa cgagtcgagt
     1381 ccggaaaagg gtctcgccga aatcctgatc gcaaagcaac gcaacggccc gaccggaacc
     1441 gtgcgcgccg cctttctcga acgctacacg cgcttcgaca atttcgctcc cgactcggcg
     1501 cttcccgaga acttccgcta gtgcgtgtcc gcgcacggat cgaccgcgag gcgctggcaa
     1561 acaaccttgc gctgctgcgg aaactcgcac cggcgagccg catcctcgcc gtcgtcaagg
     1621 cggacggcta cgggcacggc ctgatccatg ccgcgcaggc gctcgcgacc gccgacggct
     1681 tcggcgtcac cagcgtggaa gaggcgctcg agttgcgcga gagcggtttt cgcggcaccg
     1741 tggtggtact cgaaggcgca ctcgcgggca aggaactgaa ggctgccgcc gctgccagac
     1801 tcgatctcgt cgtccacgcc gattggcaac tcgacatgct ggagcgggac gggagccccg
     1861 cgaacgcacg attctgggtg aagttcgaca ccggcatgca tcggctcggc ttcccgaccg
     1921 cgacggccga tcgcgtgcgc acgcgcctcg atgcgatcgg cgccgcgaac acggtgctca
     1981 tgagccatct cgcctgcgcc gacgaaccgt cgcgtccgga aacggagcgg caattgcagg
     2041 ccttcgatcg acttcgcggg ttcttccccg gcgccgcgag ccttgccaac agcgccggcc
     2101 tgctcgccca tccggcgacg cattgcgatt gggtgcgtcc cgggctcgcg ctctacggtg
     2161 tctcgccggt cgcgggacgc gcggcgggcg aattcggctt gcggccggcg atgacgctca
     2221 cgagccggat tctgagcatc cgcgaagtgg ccgccggcga agcggtcggg tacggcgccg
     2281 cctggcgcgc cacgcgggca agccgcatcg gaatcgtcag catcggctac ggagacggct
     2341 atccctggcg aagcatttcc accggtttcg cgctggttgc cggacgcagg attccgctcg
     2401 cgggccgggt ttcgatggac atgctcggtc tcgatctcac ggaactcccc gatgttggtc
     2461 cgggcgatgt ggtcacgctc tggggcgagg gcctgccggt cgaggaggtc gcgagggctg
     2521 cgggcacgac tccctacgaa c
//
LOCUS       DASUJG010000016         3062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_616280, whole genome shotgun sequence.
ACCESSION   DASUJG010000016 DASUJG010000000
VERSION     DASUJG010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616280"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1560
                     /locus_tag="VFX38_00280"
     CDS             <1..1560
                     /locus_tag="VFX38_00280"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5313333.1"
                     /translation="NVFAVENEVAETIATALQVKLLPQESARFAQVATGDPQAYDLFL
                     KGNYYANRAFAHGDTKTPQATVSRAAALYREAVARDPRFALAWARLSLLESRAWWYGA
                     DTADARIGEAERTAKKALALDPDLPQTHMAMGFAAYYGHQDYPAALAQFEQAARGLPN
                     DADVMAAIASIHRRQGGWELALAEFQQAATLDPRNPRRYYETGVTLTELRRYAEADRQ
                     FNQALMIEPRDYMVRAYRIRLAFLSGETARARRELAAIPPDADPRGSFAALRFEFAWL
                     ARKPDDAIAALASAPAWVSSAFAEYEVPTNLLRARAWSMKGDEAHARRAYEEARATLR
                     NALRQQPDRPVFWGAFGMTEAGLGDKQAAVGAGGRAVQLVPVSGDAFYGSAHLAVLAA
                     IYAQTAAAGPAVQLLGKLLAMPSGGTISVPLLRLDPIWDPIRKSPAFQSLLKIYQHKE
                     GTAATTTSRRQRQDIRSEFTRHSRRYGRAACSDYEANLLMVAARSRSTAARTAQLATW
                     SFTKPAACMVA"
     gene            complement(1449..2033)
                     /locus_tag="VFX38_00285"
     CDS             complement(1449..2033)
                     /locus_tag="VFX38_00285"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX5313334.1"
                     /translation="MRCFGEGDALYEQYHDAEWGRPVTDERGMYERLCLEAFQAGLSW
                     RTVLHKREAFREAFVGFDPERVARYGGRDLARLMKNEGIIRNRLKIEAAVSNARALLA
                     LRDAGGSIVQLIWSFAPTRSPRRRAVADMPAQTPASQAMSKALRAKGFRFVGPTTAYA
                     TMQAAGLVNDHVASCAVRAAVERERAATIKRFAS"
     gene            complement(2048..>3062)
                     /locus_tag="VFX38_00290"
     CDS             complement(2048..>3062)
                     /locus_tag="VFX38_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008482501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HEX5313335.1"
                     /translation="GERKGFIAVFILTLQGLVFFVFYQQMSTSLTLFALRNVNLDFFG
                     YHIPPEQFQVLNPFWIWVLSPILAVIYNRLGKRDKDLNIATKFAFGFVMLAVGFFIYG
                     VSGHVGASNIISPWWLIVGYFFQSFGELLISGLGLAMVARFVAPRLRGFIMGGWLLTT
                     GISQFFGSMVATAASVPDTIGKVLGNVPASDRALFARLPTSLAHRISGFDLSAFRYWL
                     AGNPVHADALKSAPQIVQHNFGNFSESVQHTLQQMPAAASLPQSTQQALTQLNQDFYH
                     STLILYTNLFLILGVVAVGVALISFGLIPYLKRLAAGHASGIKPESAVLPEHEAGDLP
                     GGH"
BASE COUNT          598 a          953 c         1024 g          487 t
ORIGIN      
        1 aatgtcttcg ccgtcgagaa cgaagtcgcc gagacgattg ccaccgccct gcaagtgaag
       61 ttgcttccgc aggagtcggc gcgtttcgcc caggtcgcga ccggcgatcc gcaagcctac
      121 gatttgtttc taaagggaaa ctactatgcg aatcgggcgt ttgctcacgg cgatacgaag
      181 accccgcaag cgaccgtgag cagggccgcc gcgctctacc gcgaggcggt cgcccgcgac
      241 ccgcggttcg ctctcgcctg ggcgcgtctc tcgctgttgg aaagccgcgc ctggtggtat
      301 ggcgctgaca ccgcggatgc acgcataggc gaggcggagc gaacggcgaa aaaggcgctt
      361 gcgctcgatc ccgatctgcc gcagacacac atggcgatgg gatttgccgc gtattacggc
      421 caccaggact atccggccgc gctcgcgcaa ttcgaacagg ccgcgagggg cctgcccaac
      481 gacgccgacg tgatggcggc gatcgcttcc atccaccggc gccagggcgg atgggagctg
      541 gcactggcgg aattccagca ggcggcgaca ctcgatccgc gcaacccgcg gcgctactac
      601 gaaaccggcg tcacgctgac ggaattgcgc cgctacgccg aagcggatcg acaattcaat
      661 caggcgctga tgatcgagcc gcgggattac atggtccggg cctaccggat acgcctcgcg
      721 tttctgagcg gcgaaacggc ccgggctagg cgcgagctgg cggcgattcc gcccgacgcc
      781 gatccgcggg gatcgttcgc cgcgctgcgc ttcgaattcg cgtggctggc gcgcaagccc
      841 gatgatgcca tcgccgcgct cgcctccgca cccgcctggg tgtcgagcgc gttcgcggaa
      901 tacgaagtgc cgacgaactt gcttcgggcg agggcctgga gcatgaaggg cgacgaggct
      961 cacgcccgcc gcgcctatga agaggcccgc gcgacgctgc gcaacgcatt gcgacaacag
     1021 cccgacaggc cggttttctg gggcgcgttc ggcatgaccg aggccgggct cggcgacaag
     1081 caggcggccg ttggcgccgg aggccgggcc gtgcaattgg tgcccgtttc cggggatgcg
     1141 ttttatggca gtgcccatct ggcagtgctg gccgcaattt atgcgcagac cgctgcggcc
     1201 ggaccggccg tgcaactgct cggaaagctt ctcgccatgc cgtcgggggg aacgatttcg
     1261 gtgccgctgc tgcggctcga cccgatctgg gatccgatcc gaaagagccc ggcgtttcaa
     1321 tccctgctga aaatatatca gcacaaggaa gggaccgcgg ccacgacgac atcgcgtcgg
     1381 caacggcagg acatccgaag cgaattcacc cgacactctc ggcggtacgg ccgggctgcc
     1441 tgctctgact acgaagcgaa ccttttgatg gtcgcggcgc gttcgcgctc gaccgcggcg
     1501 cgcaccgcgc agctcgcgac gtggtcgttc accaaacctg cggcctgcat ggtcgcatag
     1561 gccgtcgtcg gccccacgaa acgaaagccc ttcgcgcgca gcgccttcga catggcttgc
     1621 gatgccggcg tctgcgcggg catgtcggcc acggcgcgac gccgaggcga gcgcgtaggc
     1681 gcaaaggacc agatcagttg cacgatcgaa ccgccggcgt cgcgcaatgc gagcagcgcg
     1741 cgggcattgg aaacggcggc ctcgatcttc agccggttgc ggatgatgcc ttcgtttttc
     1801 atcaaccgcg caaggtcgcg gccgccataa cgcgcgaccc gctccgggtc gaacccgacg
     1861 aaggcctcac gaaaggcctc gcgtttgtgc agcaccgtgc gccaggaaag cccggcctgg
     1921 aacgcttcga gacagagccg ctcgtacatc ccgcgctcgt cggtgacggg gcgcccccac
     1981 tccgcatcgt gatactgctc gtagagcgcg tcgccttcgc cgaagcagcg catcgagtca
     2041 gaaacgctca atgcccaccg gggagatcac cggcttcgtg ttcgggcagc accgcgcttt
     2101 cgggtttgat accggaggca tggccggctg caagtcgctt cagataggga atgagcccaa
     2161 aggagataag cgccacaccg acggcgacga cccccaggat gaggaagaga ttcgtgtaaa
     2221 gaatcagggt cgagtggtag aagtcctggt tgagctgcgt cagggcctgt tgcgtcgatt
     2281 ggggcagcga ggcggcggcc ggcatctgct gcagcgtgtg ctggacggat tcggaaaagt
     2341 tgccaaaatt gtgctgaacg atttgcgggg cgctcttgag tgcgtcggca tggacgggat
     2401 tcccggcaag ccagtagcgg aaggcgctga gatcgaaacc ggaaatcctg tgggcgagcg
     2461 aggtcgggag ccgcgcgaac aacgcccgat cgctcgccgg aacgttgccc agcacctttc
     2521 cgatcgtgtc cggaacgctg gcggcggtcg ccaccatgct gccgaaaaac tgcgagatgc
     2581 cggtcgtgag aagccagcct cccatgatga atccgcgcag tcgcggagca acgaagcgcg
     2641 ccaccatcgc gagaccgagg ccgctgatca gaagttcgcc gaaggactgg aaaaagtacc
     2701 cgacgatcag ccaccagggc gagatgatat tgctcgcgcc gacgtgcccg ctcacaccgt
     2761 agatgaaaaa tccgacggcg agcatgacga agccgaaggc gaacttggtg gcgatgttga
     2821 ggtccttgtc gcgcttgccg agccggttgt agatgacggc caggatcggc gacagcaccc
     2881 agatccagaa tggattcagc acctgaaact gttccggcgg aatatggtag ccgaagaagt
     2941 cgagatttac gttgcgcagg gcgaacagcg taagcgacgt ggacatctgc tggtagaaga
     3001 cgaagaacac gagaccctgg agggtgagaa tgaagaccgc gatgaaaccc ttgcgttcgc
     3061 cg
//
LOCUS       DASUJG010000017         3121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1107437, whole genome shotgun sequence.
ACCESSION   DASUJG010000017 DASUJG010000000
VERSION     DASUJG010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107437"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VFX38_00295"
     CDS             <1..281
                     /locus_tag="VFX38_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX5313336.1"
                     /translation="QIEALARKGDALVAISTSGGSPNVLAAVHAAHSAGAWVLAITGR
                     DGGKLAAALGPDDSELRVPAERTAHIQEVHIVLLHCLCDLVDETLYPA"
     gene            278..1282
                     /locus_tag="VFX38_00300"
     CDS             278..1282
                     /locus_tag="VFX38_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5313337.1"
                     /translation="MSLPLAGDRVAAAPPFGVPRAIATGIEWLRLPLPFPPHHVNVYR
                     IASDGNRFLVDTGFRDEATTGLWRALGMPAADRVIVTHFHPDHIGQAAAFEAAGARIY
                     LAAAELTQARALCALADAEMETIFTNFFASQGLRMPAEVVDGNRYRRAVPALPARAGE
                     LRTGPVPFAGEWRADFAGGHSPAHALLFRAEPPALAAGDILLPDITPNISVWPDDPEA
                     DPLAHYLQALARLRELPPETLVLPAHGNPGCGLHERVAAIEEHHRRRLNLLREAARGG
                     RSLRGADAIAILFPADLKAASLPFALGEALAHLNRLWHAGEFERIRDAAGIFCYAPRG
                     "
     gene            1351..1908
                     /locus_tag="VFX38_00305"
     CDS             1351..1908
                     /locus_tag="VFX38_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020412964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsinothricin resistance N-acetyltransferase
                     ArsN1 family B"
                     /protein_id="HEX5313338.1"
                     /translation="MAENIPSEVRFDSAGGGDCRIRPIVPADAAAIADIYNHYILSTV
                     ITFEEEAVTAPQIAARIEEVRRADLPWLVAESDGVLLGYANATKWKGRCAYRHSAESG
                     IYLASEATGRGIGTRLYRELLAALARRGVHAVIAGIALPNPASIALHEKCGFGKIGHF
                     REVGFKFERWIDVGYWQLLADRKDS"
     gene            complement(1925..>3121)
                     /locus_tag="VFX38_00310"
     CDS             complement(1925..>3121)
                     /locus_tag="VFX38_00310"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX5313339.1"
                     /translation="GASEREARRLSGQPERLIGHDDPPPQNANGQSPAHSPRQSLRRD
                     YLLWTFVAAFVVLIALAPGEITGVVALVNWPTIATLLGLMILTKGIERSGALHGIARA
                     VVRRMASERRLAFFLVCGSALAAMVLTNDIALFVVVPLTLGVGEFAQLPLRRLVTFEA
                     LAVNAGSLLTPIGNPQNIFLWQKSGVEFGGFVAHMLPPFLIVGILLLVLVGFAFSPRP
                     IRVVSVDGSPPLHKPLLLTAAILYAPFVVLADLHHPAIALGAVALVFLLAFPRLLRRI
                     DWSLMLVFVLMFADLGLLSRYHPLGALDLSRPGTLYLTGALLSQFISNVPAAILLAKH
                     SSDWGIIAWGANIGAFGTVIASLANLIALRLGRQRGSLLAFHAWSLPFFALAAALGWL
                     VLQFYR"
BASE COUNT          553 a         1010 c         1078 g          480 t
ORIGIN      
        1 aacagatcga ggcgctcgcg cgcaagggcg acgcgctcgt cgccatctcc accagcggcg
       61 gctcgcccaa cgtgctcgcc gccgtgcatg cggcgcactc cgcgggcgcc tgggtactcg
      121 cgatcacggg gcgcgacgga ggcaaactcg ccgccgccct cggcccggac gacagcgaac
      181 ttcgcgtccc cgccgagcgc acggcgcaca ttcaggaagt gcacatcgtg ctgctgcact
      241 gtctttgcga tctcgtcgac gagacgctct acccggcgtg agtcttccgc tcgccggcga
      301 tcgcgtggcc gccgctccgc ccttcggagt gcctcgcgca atcgcgacag gaatcgagtg
      361 gttgcgcctg ccgctgccgt ttcccccgca tcacgtcaac gtctaccgca tcgcgagcga
      421 cggcaaccgc ttcctcgtcg ataccgggtt tcgtgacgag gcgacgacag ggctgtggcg
      481 cgccctggga atgccggcgg ccgatcgggt catcgtgacg cattttcatc ccgatcacat
      541 cgggcaggca gccgccttcg aagccgcggg tgcgcgcatt tatcttgcgg cggcggaact
      601 gacgcaggcg cgcgccttgt gcgccctcgc cgacgcggaa atggagacga tcttcaccaa
      661 cttttttgcc tcgcagggct tgcggatgcc ggcggaagtc gtcgacggaa accgctatcg
      721 ccgcgccgtt cccgcgcttc cggcgcgggc cggcgaattg cgaacaggac cggttccctt
      781 cgcgggcgaa tggcgtgcgg actttgcggg cggacactcg cccgcgcacg ccctgctctt
      841 tcgcgccgag ccgccggcgc ttgccgccgg cgacattctt ctgcccgaca tcacgcccaa
      901 catttcggtc tggcccgacg atcccgaggc cgatccgctc gcgcactacc tccaggcgct
      961 cgcgcgcctg cgggaacttc cgcccgagac gctcgtgctt cccgcacatg gcaatccggg
     1021 ctgcggcctg cacgaacgcg tcgcggcgat cgaggagcat catcggcgcc ggctgaatct
     1081 tctgcgtgaa gcagcgcgcg gaggccggtc tctcagaggt gccgatgcga ttgcaattct
     1141 gtttccggcg gatctcaagg ccgcgagtct tccgttcgcg ctcggagaag ctctcgcgca
     1201 cctcaatcgt ctctggcatg ccggagagtt cgaacggatc cgcgatgcgg ccggaatctt
     1261 ttgctacgcg cctcgcggct gagaagttgc ggcccgattc ggcttcgctg cgaccatccg
     1321 gaatccggcg cgcggcgccg gacttgacga atggctgaga acatcccatc cgaagtacgg
     1381 ttcgattccg ccggtggcgg cgattgcagg atccgcccga tcgtgcccgc ggatgccgcg
     1441 gcgatcgcgg acatctacaa tcattacatt ctgagcaccg tcattacctt cgaggaagag
     1501 gcggtaacgg cgccgcagat tgccgcgcgc atcgaggaag tgcgccgtgc cgatctgccg
     1561 tggctcgtcg ccgaaagcga cggcgtgctc ctcggttacg ccaatgcgac gaagtggaag
     1621 ggccgctgcg cctaccgcca ttcggcggaa agcggcatct atctcgcgag cgaagcgacc
     1681 gggcgcggca tcggaacccg gctctatcgg gaacttctcg cggcgcttgc ccgacggggc
     1741 gtgcatgcgg tcatcgccgg gatcgcgctg cccaatccgg cgagcattgc gcttcacgaa
     1801 aaatgcggct ttgggaagat cggccacttc cgcgaagtcg gcttcaagtt cgagcgctgg
     1861 atcgacgtcg ggtactggca attgctcgcc gaccgcaaag attcctgagc aggcctgctt
     1921 cgtcctagcg gtagaattgc aaaaccagcc agccgagcgc ggcggcgagc gcgaaaaacg
     1981 gcagcgacca ggcgtggaag gcgaggagac ttccgcgttg gcgcccgaga cgcagcgcga
     2041 tcaggttcgc aagcgaagcg atgacggtgc cgaacgcacc gatgttggct ccccaggcga
     2101 tgatgcccca gtcgctcgag tgtttggcga ggagaatcgc cgccggcacg ttgctgatga
     2161 actgcgagag cagggcgccc gtgagataca gggtgccggg gcgggagagg tcgagggcgc
     2221 cgagcgggtg gtagcgggaa aggagcccca ggtcggcgaa catgagtacg aagaccagca
     2281 tcagcgacca gtcgatgcgg cgcagcaggc gcgggaatgc gagaagaaag acgagggcga
     2341 cggcgccgag cgcgatcgct ggatggtgca gatcggcgag cacgacgaag ggcgcataca
     2401 ggatcgcggc ggtgaggagc agcggcttgt gcaggggagg actgccatcg acggaaacga
     2461 cgcgaatcgg ccgcggagaa aacgcgaagc cgacaagcac gagaagcagg atgccgacga
     2521 tcagaaacgg cggcagcatg tgggcgacga agccgccgaa ttcgactccc gacttctgcc
     2581 acaggaagat gttctgcgga ttgccgatgg gagtgagcag cgagcccgcg ttcaccgcga
     2641 gcgcttcgaa ggtcacgaga cggcgcagcg gcagttgcgc gaactcgccg acgccgagcg
     2701 taagcgggac gacgacgaag agcgcgatgt cgttggtgag gaccatcgcg gccagcgcgg
     2761 aaccgcagac aagaaagaag gcgaggcgcc gctcgctcgc catgcgcctg acgacggccc
     2821 gcgcgatgcc gtgcagggca ccgctcctct cgatgccctt ggtgaggatc atgagcccga
     2881 gaagcgtcgc gatcgtcggc cagttcacca gtgcgacgac gccggtgatc tcgcccgggg
     2941 cgagcgcgat caggacgacg aaggcggcga cgaacgtcca cagcagatag tcgcggcgca
     3001 gcgattgtcg cggcgaatgc gccggggatt gcccgttcgc gttttggggc ggcggatcgt
     3061 cgtggccgat gagtcgctcc ggttggccgg aaagccggcg ggcctcccgc tcgctcgcgc
     3121 c
//
LOCUS       DASUJG010000018         7901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_985610, whole genome shotgun sequence.
ACCESSION   DASUJG010000018 DASUJG010000000
VERSION     DASUJG010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7901
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985610"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VFX38_00315"
     CDS             complement(<1..428)
                     /locus_tag="VFX38_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5313340.1"
                     /translation="MTRAIDIRAATKRFGSHTAVNELSLEVPLAVIYGFLGPNGAGKT
                     TTLRMITGILEPDSGSVTVLDGSRPAAARNRIGYLPEEKGLYKDMRVAEIVAYFGELK
                     GLSHREALARAKDRLAAAGLGDWLNSKCNALSKGMAQKVQV"
     gene            549..2240
                     /gene="ggt"
                     /locus_tag="VFX38_00320"
     CDS             549..2240
                     /gene="ggt"
                     /locus_tag="VFX38_00320"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX5313341.1"
                     /translation="MLRPAVAFLVLLFVAAGGANAATPAPALASQGMVATAEHLATGV
                     GVDVLRHGGNAVDAAVAVGYALAVVDPCCGNLGGGGFMTLHLADGKNLFLNFREKAPL
                     AASANMFLGPHGKVEQGKSLRSWQAIGVPGTVMGLDAALARYGTMSRAEVMAPAIRLA
                     REGYVLEAGDAELFAAGSERLARDPATAKIFLPHGHVPRTGDRFRQPELARTLEIISR
                     DGDRAFYAGPIAKAIVAASEANGGILSLKDFRSYTVEWERPVGCEYRGYEIVSAPPPS
                     SGGITLCEILNVLAGWPEFAGYEWHSVPAVHDMVEAMRFAYADRNRYLGDPDFVKIPV
                     AKLLSARHAAAIRARIPADRAVPSSSIHVDLAAAEGTHTTHYSIVDAKGNAVAVTYTL
                     NSWFGTGFVAPGTGFLLNNEMDDFTAKPGVPNQYGLVQGEANAIAPGKRPLSSMTPTI
                     VLKNGKVFMVTGSPGGSTIIATVLETILNVVDRGMNVRQAVDAPRIYQQWLPDVVHVE
                     PGALTPAVEQALEKMGYRFEVWDSRGAAEAIVVLPDGELSGGSDRRRPAGLAAGY"
     gene            2417..3649
                     /locus_tag="VFX38_00325"
     CDS             2417..3649
                     /locus_tag="VFX38_00325"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5313342.1"
                     /translation="MRVIVLGAGIAGVTTAWYLVAAGAEVTLLDRGLEPASETSHANG
                     GHISTQSCAPWTGPAGLREFFATRLRRSPAIGYRHAREPARRRWFAQALAASRASRYR
                     RGCAVIARLARYSRELFEGLIAEQGIDVALESDGVLALYRHERVFARACRRLPAGVEI
                     LSPDEVLAREPALAQATLRPVGALAYPGDATGDCRAFCVALAERAAARGLRFLYRTTV
                     DGLILESGRCLGVRSDGGDLRADAVVVALGAWSANFLRPCGLRLPIVALRGYTLTSPI
                     AAGAVVPGRFHDVERHIVGARLGGSFRAAGMADFAGFDATIAAARVARLEQVVREWYP
                     ALSRTAIERWSCLRPITPDGAPILGASPIAGLWLNTGCGPYGWTLGCGAGKLVADLLL
                     ESSPAIPLDGMSAGRYGS"
     gene            complement(3646..4305)
                     /gene="rpiA"
                     /locus_tag="VFX38_00330"
     CDS             complement(3646..4305)
                     /gene="rpiA"
                     /locus_tag="VFX38_00330"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018860546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEX5313343.1"
                     /translation="MNEDNAKRRVAETALEYLRGCEIVGVGTGSTVAHFIAALAGRKQ
                     EFDGAVASSEASANLLRKAGIRLLDLNATGPLPVYVDGADEATRHGQLIKGGGGALTR
                     EKILAAVSRRFVCMIHRPKLVDVLGGFGLPIEVIPMARSYVARELVARGGQPEWRKDF
                     ITDNGNVILDVKGLEILDPSARETELNQIAGVVTVGLFARRRADILLVGDDGGVETLT
                     P"
     gene            complement(4298..4915)
                     /locus_tag="VFX38_00335"
     CDS             complement(4298..4915)
                     /locus_tag="VFX38_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313344.1"
                     /translation="MHAIDRCDAGVARDKSSRIAFEKIFGELQHFLKNSSILRSEPST
                     HGDFAHGTSQDCFSQEDWNTSQEEDGGTQEDRPPHESDTSQGRREKACDVPEEENGTS
                     QEEGRHPKEEGRHSQAEGRHPQEKGGYPQEEGRHSQEEGRHSQEEGRYPPPQDCAAKE
                     DRFDGRDARAFRPDGLIERQDKGTRRDERRVPFSLPLEPGVAIRE"
     gene            complement(4964..5575)
                     /locus_tag="VFX38_00340"
     CDS             complement(4964..5575)
                     /locus_tag="VFX38_00340"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX5313345.1"
                     /translation="MPDFLPAELQERAILRARLRRLRAELPAREVEVATPAANARLLA
                     TPEFLKAQRIGAYVAVRGELDPATALAAALATGKEVFLPRIGPDATLHFLPWQTPIAL
                     RRNRFGIPEPVAPDESAIAPEDLDLVIVPLIGFHRDGIRLGSGGGYYDRCFAFRRNSL
                     PRPRLAGFAYALQECERLRAAPWDVPLDLLVSEREVIRFDFDE"
     gene            complement(5572..5752)
                     /gene="ssrS"
                     /locus_tag="VFX38_00345"
     ncRNA           complement(5572..5752)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VFX38_00345"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(5804..6112)
                     /locus_tag="VFX38_00350"
     CDS             complement(5804..6112)
                     /locus_tag="VFX38_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEX5313346.1"
                     /translation="MPDASTQVNVRILDRDYQVACPPEERQSLIDAADLLSRRMEEVR
                     DSGRVIGMERIAVMVALNLAHQYLEAEGERGAVSGDTLKRLRELRLQLAAVGAEQADD
                     "
     gene            complement(6118..6345)
                     /locus_tag="VFX38_00355"
     CDS             complement(6118..6345)
                     /locus_tag="VFX38_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02449 family protein"
                     /protein_id="HEX5313347.1"
                     /translation="MAANVSRQLDAEIAAIEREVLRLSDIARRLREENRLLAERLNGL
                     NSERAQLLNKNAIVQSRVEAMIARLKGMEQA"
     gene            6521..7828
                     /gene="pepP"
                     /locus_tag="VFX38_00360"
     CDS             6521..7828
                     /gene="pepP"
                     /locus_tag="VFX38_00360"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HEX5313348.1"
                     /translation="MNPQEFQRRRRALMRMMGRSSIAILPSAREKMRNRDVAYPFRQD
                     SDFLYLSGFGEPDAVAVLIPGRAVGDYVLFCRERDPEQETWTGYRAGPEGAVRDHGAA
                     DAFPIGDLDEILPGLIEPCERVFYTMGLFPEFDRRMVGWLNQLRASGRSGARTPQAFV
                     SLDHFLHDLRLYKSRSEIAAMKRAARISVEAHRRAMRRAAPGLFEYEVEAELAGCFRA
                     HGNREAYGSIVGSGANSCVLHYVANDRRMTDGDLLLIDAACEEECYAADITRTFPVNG
                     RFTPEQRAIYELVLAAQKAAIDKCRPGRRFNEPHEETVRVITRGLVKLGLLRGTPAKL
                     IKEEAYKKFYMHRAGHWLGLDVHDVGDYKIGDEWRELEPGMVLTVEPGIYIAPGTKGV
                     AKKWWNIGVRIEDDVLVTRGAPEVLTAALVKEADEIEELMQAA"
BASE COUNT         1236 a         2602 c         2649 g         1414 t
ORIGIN      
        1 acctgcacct tctgcgccat gcccttggat agcgcattgc acttggagtt cagccagtcg
       61 ccgagcccgg ccgccgcgag gcgatctttc gcccgcgcga gggcctcgcg atgcgagagc
      121 cccttgagtt cgccgaagta ggcgacgatt tcggcgaccc gcatgtcctt gtagaggcct
      181 ttttcttccg ggagataacc gatgcggttg cgtgccgcgg cgggccggct cccgtcgagc
      241 accgtcacgc tgccggaatc gggctcgaga atgccggtaa tcatccgcag cgtcgtggtc
      301 ttgccggccc cgttcgggcc gagaaaaccg tagatgacgg ccagtggcac ctcgagcgaa
      361 agctcgttga ccgccgtgtg ggaaccgaaa cgcttggtcg cggcgcgaat gtcgatggcg
      421 cgggtcatgc tgcggaccgc gacgaagtgc gcgaattata acgaagcggg gtcgcggtcg
      481 gaggcggcgt tgcccgagag cgcccggcgt ccgttagact gatagccgtg attacccgcg
      541 ccgccagcat cctgcgcccc gccgtcgcat tcctggttct cttgttcgtc gcggccggcg
      601 gcgcgaacgc cgcgacgccc gcgcccgcgc ttgcatcgca ggggatggtc gcaaccgccg
      661 agcatctcgc gaccggggtc ggcgtggatg tgctgcgtca cggcggcaac gccgtcgatg
      721 ccgcggtcgc ggtcggctat gcgctcgccg tcgtcgatcc ctgttgcggc aatctcggcg
      781 gcggcggctt catgacgctt catctcgcgg atggcaagaa tctctttctc aattttcgcg
      841 agaaggcgcc gctcgccgcg agcgcgaaca tgtttctcgg cccccacggg aaagtcgagc
      901 agggcaaaag tctccggagc tggcaggcga tcggcgtgcc gggcacggtc atggggctcg
      961 acgctgcgct cgcgcgctac ggaaccatga gccgcgccga agtcatggcg cccgcgattc
     1021 ggctcgcgcg cgagggctac gtgctcgaag cgggcgatgc cgagcttttc gccgccggct
     1081 ccgagcgcct cgcacgcgat cccgccaccg cgaagatctt tctcccgcac ggccatgtgc
     1141 cgcggacggg cgaccgcttc cgtcaaccgg aactcgcccg cacgctcgaa atcatttccc
     1201 gcgacggcga cagggccttc tacgcgggtc cgatcgcgaa ggcgatcgtc gcggcctcag
     1261 aggcgaacgg cggaatcctg agtctcaagg atttccgctc ctacacggta gagtgggagc
     1321 gacctgtcgg ctgcgaatat cgcggctacg aaatcgtgtc ggcgccgccg ccgagttcgg
     1381 gcggcatcac cctgtgcgag atattgaacg tgctcgccgg ctggcccgag ttcgccggtt
     1441 acgaatggca ttcggtaccc gcggtgcacg acatggtgga ggcgatgcgc ttcgcctatg
     1501 ccgatcgcaa ccgctatctc ggcgatcccg actttgtgaa aattccggtc gcgaagctcc
     1561 tgtcggcgcg ccatgcggcc gcgatccgcg cccgtatccc cgccgaccgc gcggtgcctt
     1621 cgagttcgat ccacgtcgac ctcgcggcgg cggaaggaac gcacaccacg cattattcga
     1681 tcgtggatgc gaagggcaac gcggttgccg tgacctatac cctcaattcc tggttcggca
     1741 ccggcttcgt cgcgcccggg accggttttc ttctcaacaa cgagatggac gacttcaccg
     1801 cgaaacccgg cgtgccgaat cagtacggcc tcgtgcaggg cgaggccaac gcgatcgcgc
     1861 cggggaagcg cccgctttct tcgatgacgc cgacgattgt gctcaaaaat ggaaaggtgt
     1921 tcatggtgac gggcagcccc ggcggctcga ccatcatcgc cacggtgctc gagacgatcc
     1981 tgaacgtcgt ggatcgcggc atgaacgtcc gccaggcggt cgacgcgccg cgcatttacc
     2041 agcaatggct gcccgatgtc gtccacgtcg agcccggcgc gctcacgcct gcggtcgagc
     2101 aggcactcga gaagatgggc tatcgcttcg aggtttggga ctccaggggt gcggcggagg
     2161 ccatcgtcgt tcttcccgac ggagagctga gcggcggcag cgaccggcga cgtcccgcgg
     2221 gtctcgccgc cgggtattga acggccgccg aatcggcggc tacgtcgctg gtaccgaagc
     2281 aatggcaaga acggtctact cgaatgacat tttctctctt gccatgaagg actcgcgcgg
     2341 caatcctcgc accgcgatcg gcaacgcaga caatccgccg ccggatcgcc cgcgggcggc
     2401 aagggggcgc tgagcggtgc gggtcatcgt tctcggcgcc ggcatcgcgg gcgtcaccac
     2461 cgcctggtat ctggtcgcgg ccggagcgga ggtgacgctg ctcgaccgcg gcctcgaacc
     2521 ggcgtcggaa accagccacg cgaacggcgg ccacatctcg acgcagagtt gtgctccctg
     2581 gaccgggccc gcgggcctgc gcgaattctt cgcgacgcgc ctgcgccgtt cgccggccat
     2641 cgggtatcgg cacgcacggg agcccgcgcg ccgccgctgg ttcgcacagg cgctcgccgc
     2701 ctcgcgggct tcccgttacc ggcgcggctg cgccgtgatc gcgaggctcg cgcgctacag
     2761 ccgtgagttg ttcgaaggct tgatcgcgga gcagggaatc gacgtcgctc tggagtcgga
     2821 cggcgtgctc gcgctctatc gtcacgaacg cgtcttcgcc cgcgcatgtc gccggctgcc
     2881 tgcgggagtc gaaattcttt cccccgatga agtgctcgcg cgtgaaccgg cgctcgccca
     2941 ggccacactg cgcccggtcg gggccctcgc ttatcctggc gatgctaccg gtgattgccg
     3001 cgcattctgt gtcgctctcg ccgagcgtgc tgcggcccgc gggcttcgct ttctttatcg
     3061 cacgacggtg gacggtctga tcctcgagtc ggggcgttgc ctcggcgtgc gcagcgacgg
     3121 cggcgacttg cgcgccgatg ccgtggtcgt cgcgctcggc gcatggagtg cgaactttct
     3181 tcgtccctgc ggcctgcgtt tgccgatcgt cgcgctgcgc ggctacacct tgacgtcgcc
     3241 gattgctgcc ggcgccgtgg tgccgggccg tttccatgac gtcgaacggc acattgtcgg
     3301 cgcacgcctc ggcgggagtt ttcgtgccgc cggcatggcg gactttgccg gtttcgatgc
     3361 cacgatcgcg gcggcgcgcg tcgcccgcct cgagcaggtc gtgcgcgaat ggtatccggc
     3421 cctttcgcgc accgcgatcg aacgctggag ctgccttcgt ccgatcaccc cggacggtgc
     3481 gccgatcctg ggagcgagcc cgatcgcggg attgtggctc aacaccggtt gcggacccta
     3541 cggctggacg ctcggttgcg gcgccgggaa actggtagcg gatctcctgc tcgaatcgtc
     3601 gcccgcgatt ccgctcgacg ggatgagtgc cgggcgctac ggatcctagg gcgtgagagt
     3661 ttcgactcca ccgtcatcgc cgacgagcag gatgtcggcg cggcgccggg cgaagagtcc
     3721 tacggtcacg acgccggcga tctgattcaa ctccgtctcg cgcgccgacg gatccaggat
     3781 ctcgagcccc ttcacgtcga ggatgacgtt gccgttgtcg gtgatgaagt ccttgcgcca
     3841 ttcaggctga ccgccgcggg cgaccagttc gcgcgcgaca tagctgcgcg ccatcgggat
     3901 cacctcgatc ggcagaccga aaccgccgag gacatccacg agtttcggcc ggtggatcat
     3961 gcagacgaaa cgcctggaca cggcggcgag aatcttctcg cgggtgaggg cgccgccgcc
     4021 gcccttgatg agttgcccgt gacgcgtcgc ctcgtccgcg ccgtcgacgt agaccggaag
     4081 cgggccggtt gcgttgagat cgaggagacg gattcctgcc ttacgaagga ggttcgcaga
     4141 cgcttcggaa ctcgcgactg cgccgtcgaa ttcctgcttg cgtccggcca gcgcggcgat
     4201 gaaatgcgcg accgtcgagc cggtgccgac tccgacgatt tcgcagccgc gcagatactc
     4261 gagcgccgtc tcggcgaccc gccgtttggc gttgtcctca ttcacggatg gcgacgcctg
     4321 gttcgagggg aagcgaaaaa ggcacgcgcc gctcgtcgcg acgcgtgccc ttgtcctgcc
     4381 gttcgatcag gccatcgggc cgaaaagccc gggcgtcccg gccatcgaag cggtcttctt
     4441 tcgccgcgca gtcctgcggc ggcgggtacc ggccttcttc ttgcgagtgc cggccttctt
     4501 cttgcgagtg ccggccttct tcttgcgggt acccgccttt ttcttgcggg tgcctgcctt
     4561 ccgcttgcga gtgccggcct tcttctttcg ggtgccggcc ttcttcttgc gacgtaccgt
     4621 tttcttcttc cgggacgtcg cacgcttttt cgcgacgacc ttgcgacgtg tcgctctcgt
     4681 gcggcgggcg gtcttcttgc gtgccgccgt cttcttcttg cgacgtgttc cagtcttctt
     4741 gcgagaagca gtcttgcgag gtgccatgag caaagtctcc gtgtgtggat ggctcgctcc
     4801 taagtataga agaatttttc aaaaagtgtt gcaattcgcc aaaaattttt tcgaatgcga
     4861 ttcgtgaaga tttgtcgcga gcgactccgg cgtcgcaccg atcgatcgca tgcatgtgag
     4921 tgacggcggg attttgcatc gcggtttgcg acgcccgaaa acctcactcg tcgaagtcga
     4981 aacggatcac ttcgcgttcg cttaccagca gatcgagcgg cacatcccaa ggcgccgcac
     5041 ggagcctctc gcactcctgc agcgcgtagg cgaaacccgc gagacgggga cgcggaagcg
     5101 aatttctccg gaacgcgaag cagcgatcgt agtatccgcc gccgcttccg agtcggattc
     5161 cgtcgcgatg aaacccgatc aggggaacga tgacgaggtc gagatcttcc ggtgcgatcg
     5221 cggactcgtc gggcgccacg ggttccggaa tgccgaagcg attgcggcgc agcgcgattg
     5281 gtgtttgcca cggcagaaag tgcagggtgg cgtccgggcc gatccgcggg agaaacactt
     5341 ccttgccggt ggcgagcgcg gcagcgagcg ccgttgccgg atcgagttcg ccgcgtacgg
     5401 cgacgtaggc gccgatgcgt tgcgccttca ggaattcggg agtggcgagg agccgggcgt
     5461 tggccgccgg cgtcgcgacc tcgacttcgc gcgccggaag ttccgcccgc agccgccgca
     5521 ggcgcgcgcg gaggatcgcg cgctcctgca gttcggcggg gaggaaatca ggcatcctcc
     5581 gctcgcgccg tcaccagcgg tcgtcctcga accgaaggtt caagggggag cgggccccga
     5641 tgccgaaggc tttccgcaac gcggcatagc acagatgaca gcggcggcca ggctccaacg
     5701 cacacgatta ggctcgaagg gtttgcgcca gggtcgtgcg cagcagagga tgcggcgtca
     5761 tcttcgcgcg attgcggccg cgacgcaagc ccccgctcgc gggtcagtcg tccgcttgct
     5821 ctgcgccgac cgcggcaagc tggaggcgca actcgcgcag gcgcttgagc gtgtcgccgc
     5881 tcacggcgcc gcgttctccc tccgcctcga gatactggtg cgcaaggttg agcgccacca
     5941 tgaccgcgat gcgctccatg ccgatgacgc ggccggaatc gcgaacctct tccatgcggc
     6001 ggctgagaag atcggccgca tcgataagcg attggcgctc ctcgggaggg caggcgacct
     6061 ggtagtcgcg gtcgaggatg cggacgttca cctgggtgct tgcgtcgggc atcgcgtcta
     6121 tgcctgttcc atgcccttca gccgggcgat catcgcctct acccggcttt gcacgatggc
     6181 gttcttgttg aggagttgcg cgcgctcgga gttcagtccg ttgagccgct ccgcgagcag
     6241 ccggttctcc tcgcgcaggc gccgggcgat gtcgctcagg cgcaacacct cacgttcgat
     6301 cgcggcgatt tcggcgtcga gttgtcggga gacgttggcg gccatcggct caactatagg
     6361 cataaacgtc gcccgaatca acaagcgacg caggcgtgat cagccgcccg gttgtgacgg
     6421 cgcgggcgcc ggtaccggaa acggcgtcgc cgtgcgctcc ggtcgcgccg cgcgcggcgt
     6481 ggcacaatgg aacgtgccca ccgcaaagga acactcgttc atgaacccgc aggagtttca
     6541 gcgccgccgc cgcgcattga tgcgcatgat ggggcgcagt tcgattgcga ttctgccttc
     6601 ggcgcgcgag aaaatgcgca atcgcgatgt cgcctatccc ttccgccagg acagcgattt
     6661 tctctatctc agcggattcg gcgaacccga cgctgtcgcc gttctcatcc ccgggcgcgc
     6721 ggtcggcgac tatgtgttgt tctgtcgcga acgcgatccg gaacaggaaa cctggaccgg
     6781 ataccgggcg gggcccgaag gggcggtgcg cgaccacggg gcggccgacg cctttccgat
     6841 cggcgatctg gacgagattc tgcccggcct gatcgaaccc tgcgagcgcg tgttctacac
     6901 catgggactc tttccggaat tcgaccggcg catggtcggc tggctcaatc agttgcgcgc
     6961 aagcggccgc tcgggggcgc gcacgccgca ggccttcgtt tccctcgatc atttcctcca
     7021 cgatcttcgc ctctacaaga gccgcagcga aatcgcggcg atgaagcgcg ccgcgcgaat
     7081 ttcggtcgag gcgcatcggc gcgccatgcg gcgcgccgcc ccgggactct tcgaatatga
     7141 agtcgaagcg gaactcgccg gctgtttccg cgcccacgga aaccgcgaag cctacggctc
     7201 gatcgtcggc tccggcgcca acagttgcgt cctccattac gtcgcgaacg accggcggat
     7261 gacggacggc gatctgctgt tgatcgacgc cgcctgcgaa gaggagtgct acgccgcaga
     7321 catcacccgc accttcccgg tcaacggccg tttcacgccc gagcagcgcg cgatctacga
     7381 actggtgctc gccgcccaga aggctgcgat cgacaaatgc cgaccggggc ggcgcttcaa
     7441 cgagccgcac gaggagacgg tgcgcgtcat cacccgcgga ctggtcaaac tcgggctgct
     7501 gcgggggacg cccgcgaagc tcatcaagga ggaggcctac aagaagttct acatgcaccg
     7561 cgccggacac tggctggggc tcgacgtgca cgatgtcggc gactacaaga tcggagacga
     7621 atggcgcgaa ctcgaaccgg gcatggtgct cacggtggag ccgggcatct acatcgcgcc
     7681 gggaaccaag ggagtcgcga agaagtggtg gaacatcggc gtgcggatcg aggatgacgt
     7741 gctcgtcacc cgcggtgcgc cggaggttct caccgcggcc ctcgtcaagg aagccgacga
     7801 gatcgaagag ctgatgcagg ccgcatgaac gcgggcgccg cccgcgacgt cgtaatcgcg
     7861 ggcggcggtc tcgccggcgc gcttgccggc ttcgcgctcg c
//
LOCUS       DASUJG010000019         2793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1474770, whole genome shotgun sequence.
ACCESSION   DASUJG010000019 DASUJG010000000
VERSION     DASUJG010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474770"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VFX38_00365"
     CDS             complement(<1..457)
                     /locus_tag="VFX38_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010893577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX5313349.1"
                     /translation="MDSPAPSAGRTGNAAFRRVVLKLSGEALMGEAEYGIDPQMLARL
                     GGEIVQAQELGVELAIVIGGGNLFRGSGLARHGMDRVTGDHMGMLATVMNGLALQGEL
                     ERRGLKVRVMSAIRINEVCEDYIRRRAVRHLEKGRVIVLAAGTGNPYFTT"
     gene            complement(475..1350)
                     /gene="tsf"
                     /locus_tag="VFX38_00370"
     CDS             complement(475..1350)
                     /gene="tsf"
                     /locus_tag="VFX38_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX5313350.1"
                     /translation="MSIGAAEVKALRERTGAGMMECKRALTESGGDPERAIEILRTAG
                     LAKADKRAGRTAAQGRIALAGDVRGAAMVEVNSETDFVAAGSDLAGFADAVAAVVLAR
                     APGDLAAVAELPLADGTTVEQSRRALVAKLGENIAVRRVARFATATGIIGRYLHGTRI
                     GVLIELDGGDEELARDVAMHVAASRPVCVSGEEIPADLLAREREIHRAQAEASGKPAA
                     IVEKMVAGRLAKYVNEVTLLGQSFVKDPDRSVAALLEAGGARAVAFARFEVGEGIEKD
                     EADFAAEVMAQVRAR"
     gene            complement(1707..2387)
                     /gene="rpsB"
                     /locus_tag="VFX38_00375"
                     /pseudo
     CDS             complement(1707..2387)
                     /gene="rpsB"
                     /locus_tag="VFX38_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534589.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
     gene            2611..>2793
                     /locus_tag="VFX38_00380"
     CDS             2611..>2793
                     /locus_tag="VFX38_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010366184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX5313351.1"
                     /translation="MAVTVKTAEEITKMRRAARLTAEVLDVIAPHIRPGVSTDDLDRI
                     CHDHIVEVQKVIPANVG"
BASE COUNT          416 a          979 c          880 g          518 t
ORIGIN      
        1 cggtcgtgaa gtagggattg ccggtcccgg cggcgagcac gatcacccgc cccttttcga
       61 gatggcgaac ggcgcgccgg cggatatagt cctcgcagac ttcgttgatg cggatcgccg
      121 acatgacccg caccttgagc ccgcggcgtt cgagttcgcc ctgcagcgca agtccgttca
      181 tcaccgtggc gagcatcccc atgtgatcgc cggtgacgcg atccattcca tggcgcgcga
      241 gcccggaacc gcggaaaaga ttgccgccgc cgatgacgat cgcgagttcg acgccgagtt
      301 cctgcgcctg cacgatctcg ccgccgaggc gcgcgagcat ctgcggatcg atgccgtatt
      361 cggcctcgcc catcaaggcc tcgccggaga gtttcagaac cacccgacga aaggcggcgt
      421 ttcccgttct tcccgcgctt ggcgccggcg agtccatggc ctggcctgcc cgcgctagcg
      481 ggcgcgtacc tgggccatga cttcggccgc aaaatccgcc tcgtccttct cgatgccctc
      541 gccgacttcg aaacgcgcga aggcgacggc gcgcgcgccg cccgcctcga gcagggcagc
      601 gacggagcga tccggatcct tcacgaagga ctggccgagc agcgtgactt cgtttacgta
      661 cttggcgagt ctgccggcta ccatcttctc gacgattgcg gccggcttgc cgctcgcctc
      721 ggcctgcgcg cgatgaatct cgcgttcgcg cgcgagaaga tcggcgggaa tctcctcgcc
      781 actcacgcac accgggcggc tcgccgccac atgcatggcg acatcgcggg cgagttcttc
      841 gtcgccgccg tcgagttcga tcagaacccc gatgcgcgtg ccgtgaagat agcgaccgat
      901 gattcccgtc gccgttgcaa aacgcgcgac gcggcgcacc gcgatgtttt cgccgagttt
      961 cgcgacgagc gcacgccggg actgttcgac cgtcgttccg tccgccagcg gcagttccgc
     1021 aacggcggcg agatcgcccg gcgcccgtgc gaggacgacg gccgcgaccg cgtcggcaaa
     1081 accggccagg tcggatccgg cggcgacgaa atcggtttcg ctgttgactt ccaccatggc
     1141 cgccccgcgc acatcacccg cgagggcgat ccgcccttgc gccgccgtgc gtcccgcgcg
     1201 cttgtccgcc ttggcgagcc cggccgtgcg cagaatctcg atcgcgcgct ccgggtcgcc
     1261 gccgctttcc gtaagcgcgc gcttgcattc catcatgccg gcgccggtgc gctcgcgcag
     1321 cgccttgact tcggcggctc caatactcac gcctcttccc cttcgtcggg cgtcgcatcc
     1381 cgaacggctt cctcctccgt cgccgattcc ggctccgcgg catcttcccc gccggcatcc
     1441 ttcgtcgccg cgaccttgtg ccgcggtttt gcctgggtcg ccgtttccgc ctcagtctcg
     1501 tctgcgcctt ccgtcacgcc ctcgccttct tcggtcccct ggggcgagga agccgccgct
     1561 ttggcggttc gggtcgagcg cttgcgtgcg gccggcctgg tggcagagtc gcctgagcgc
     1621 ggtgcacgcc cgcggcgccg gcgggtcatc ttctgcactt cctcttcttc cacgaagtcc
     1681 tcggcatcgc cctcgatgac cggcaggctt tcgcggcccg tgatgatcac gtccgccgcg
     1741 gcccgcgcaa agagttcgat cgcggaaatc gcatcgtcgt tgccgggaat gacgtagtcc
     1801 acgccatcgg gactattgtt ggtgtcgacg attccgatca cgggaatccc gagcttgttg
     1861 gcctcggaga ccgcgatttt ctcgtaaccc acatcgacga cgaagagcgc atcgggaagc
     1921 gattccatgg cacggattcc gccgaggctg gtttcgagct ttccctgctc gcggcgcagc
     1981 cggccggctt ccttcttgat cagccgctcg agcgtgccgt cctcggccat cgcctcgagt
     2041 tcgagcagcc gcttgaccga gcggcgcacg gtggcgaaat tggtgagcgt gcctcccagc
     2101 cagcgctggt tgacataggg catgttgcag cgcacggcct cccgggcgat cacgtcacgc
     2161 gcggcgcgct tggtgccgac gaagagaatt tgcgcgccct gcgcggcgag gctcgcgaga
     2221 taattctgcg cgtcctcgag gagcggcacg gtagactcga gattgatgat gtgaatcttg
     2281 cttcgatgtc cgaagatgta gggtgccatc ttcggattcc agaagcgggt ctgatgcccg
     2341 aaatgcacgc ccgcctcgag catgctgcgc atggatacgg tagccatagt ttctcctgtc
     2401 gggttgggcc tccacgcgtc ccatgcggca acccgaccgg cgaaaccgcg gagcctcctc
     2461 cgttcggtcc ggactccgcc cggcaggcga ggtcgttttc gtggtcgggc acccggccgc
     2521 atgtgccggc gcgtgtgcgg atttaacggc gtattctacc ttgttgcgcc gcaacgatac
     2581 aatgagttct gtgcatccgg ggcaccgcgc gtggccgtaa ccgtaaaaac agcggaagaa
     2641 atcaccaaaa tgcgccgcgc cgcccggctc acggccgagg tgctcgacgt gatcgccccc
     2701 cacatccgcc ccggggtgag caccgatgac ctcgatcgaa tctgccacga tcatatcgtc
     2761 gaggtgcaga aggtgattcc ggccaacgtc ggc
//
LOCUS       DASUJG010000020         2497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1107898, whole genome shotgun sequence.
ACCESSION   DASUJG010000020 DASUJG010000000
VERSION     DASUJG010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107898"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VFX38_00385"
     CDS             complement(<1..298)
                     /locus_tag="VFX38_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018623506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HEX5313352.1"
                     /translation="MNVPDPACVEIEPQASAQASVIWLHGLGADGHDFEPVVPELALP
                     RDLAVRFVFPHAPVRAVTLNGGMPMRAWFDIARLDFEGRWDEAGVAQAIERVDAL"
     gene            393..1022
                     /locus_tag="VFX38_00390"
     CDS             393..1022
                     /locus_tag="VFX38_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX5313353.1"
                     /translation="MEHVFNFLLHMDRYLAAFTQEHGLWVYALLFIVVFAETGLVVTP
                     FLPGDTLLFIAGALAGSGLISAPATAAIFTIAAILGNLSNYAIGRAIGPRVFKTRSRW
                     LNRRHLEEGHAFFERHGGKTIVLARFLPIIRTFAPFIAGVSAMSWLPFFAYTLAGAIA
                     WVAIVFTGGMFFGNLPFVRDHLTLVILGLVIVSLIPAVIFDLRRRRKRA"
     gene            1015..1722
                     /locus_tag="VFX38_00395"
     CDS             1015..1722
                     /locus_tag="VFX38_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020266165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5313354.1"
                     /translation="MPEAAKARARIALSWSGGKDSLMALLALEANPSLEVCALVTTLN
                     ESTARVSMHGLRESLLDTQAQTIGLPLVKCGLPANAGNDLYRRRFAAALAPLIDDGVS
                     GVAFGDLFLAEVRSFREAQMQALRLEALFPLWGTPSATLARLFLQSGHRAILGCVDAT
                     RLGPRFLGRDYDAKLLAELPVAVDPCGENGEFHSFVHAGPRLVRNIRFRRGRRVVHAQ
                     RFHFLDLLAEPPARAAV"
     gene            1775..>2497
                     /locus_tag="VFX38_00400"
     CDS             1775..>2497
                     /locus_tag="VFX38_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313355.1"
                     /translation="MRNCLILGSGRSGTSMLGGVLNAAGYYMGEKLYRARDANPRGFF
                     EAPAINSINEHLLRPHAASRCPYPLLRVWRSLFSAERWLARIPLGTQIASTPKIAGRI
                     AAEVARRPFAFKDPRFCYTLPAWRPQLPPDTVFLCIFREPGRTAASILRERRRKYPDL
                     RLRLEDAFGVWNDMYQHVLRIHRHSGRWLFIHYDQMINGAAISAVEKLLGVHVDASFP
                     DVAFDRASPIKTPAQARATYREL"
BASE COUNT          381 a          877 c          762 g          477 t
ORIGIN      
        1 tcagggcatc cacccgctcg atcgcctgcg cgactccggc ttcgtcccac cggccctcga
       61 aatccaggcg cgcgatgtcg aaccaggcgc gcatcggcat gccgccgttt agcgtcacgg
      121 cgcgcacggg cgcgtgcgga aacacgaagc gcaccgccag atcgcgaggg agagcgagtt
      181 cgggtacaac cggctcaaaa tcgtgtccgt ccgcaccgag tccgtgcaac cagatgacgc
      241 tcgcctgcgc actcgcctgc ggctcgattt cgacacaagc gggatcgggg acattcatcg
      301 gcgtgattgt agcgtccccg ctcgatcgcc cctcgaactt cgccgcgagc ggcatccggg
      361 atagactgtc gccgccgcat cgcggagccc ccgtggaaca cgtcttcaat ttcctgctgc
      421 acatggaccg ctatctcgcg gccttcacgc aggaacacgg gctgtgggtg tacgccctcc
      481 ttttcatcgt ggtgttcgcg gagaccggcc tcgtcgtcac cccctttctc ccgggcgata
      541 cactgctctt catcgccggc gcgctcgccg gttcgggtct catctccgcg cctgcgacgg
      601 cggcgatctt cacgatcgcg gccattctcg gcaacctgtc gaattacgca atcggacggg
      661 ccatcgggcc acgcgtcttc aagacgcgat cgcgatggct caaccggcgc catctcgagg
      721 aagggcacgc gtttttcgaa cgccacggcg gcaagaccat cgtgctcgcg cgcttcctgc
      781 cgatcattcg caccttcgct ccgttcatcg cgggggtgag cgcgatgtcc tggctcccgt
      841 tctttgctta cacgctcgca ggcgccatcg cctgggtcgc aatcgtattc accggcggga
      901 tgttcttcgg caaccttccc ttcgtgcgcg accatctcac gctcgtaatc ctcggtctcg
      961 tgatcgtctc gttgattccc gccgtgatct tcgatctgcg ccgccggcgc aagcgtgcct
     1021 gaagcggcga aggcgcgagc gcgcatcgcc ctttcctgga gcgggggcaa ggacagcctg
     1081 atggcgctcc ttgccctcga ggcgaatccg tcgctcgagg tttgcgccct cgtgacgact
     1141 ctcaatgagt ccaccgcgcg cgtcagcatg cacggtctgc gggagtcgct gctggacacg
     1201 caggcacaaa cgatcggatt gccgctggtg aagtgcggcc tgcccgccaa cgcgggcaac
     1261 gatctctatc gccgacgctt cgccgccgct cttgcgccgc tcatcgacga cggcgtgagc
     1321 ggcgtcgcat tcggcgatct ttttctcgcc gaagtccgtt cctttcgcga agcgcagatg
     1381 caggcccttc gtctcgaggc cttgtttccg ttgtggggca caccgagcgc gactctcgcg
     1441 cggctttttc tgcaatcggg tcaccgcgcg attctcggct gcgtggacgc aacccgactc
     1501 ggcccgcgct tcctcgggcg cgactacgat gcgaaactcc tcgcggagct gccggtggcg
     1561 gtcgatccct gcggcgagaa cggcgagttc cacagcttcg tgcatgcggg gccgcgtctc
     1621 gtacgcaaca tccgatttcg ccgtggccgc cgcgtcgtgc acgcgcaacg ttttcatttt
     1681 ctcgacctgc tcgcggagcc gcccgcccgc gcggccgtat aatcacgggg atggggagag
     1741 tgctcctcgc cggcccggga gagaaaagcc gccgatgcgc aactgtctga tcctcggctc
     1801 ggggcgcagc ggcaccagca tgctcggcgg ggttctcaac gcggcgggat attacatggg
     1861 cgagaagctc taccgcgccc gcgatgccaa tccccgcggc tttttcgaag cccccgcgat
     1921 caattccatc aacgagcatc tcctgcgccc gcacgcggca tcgcgctgcc cgtatccgct
     1981 gctgcgcgtg tggcgcagcc tgttttcggc ggagcgctgg ctcgcgcgca ttccgctcgg
     2041 gacccagatc gcgagcacgc cgaagatcgc ggggcgcatc gccgccgagg tcgcgcgccg
     2101 gcccttcgcg ttcaaggatc cgcgtttttg ctacacgctt ccggcgtggc ggccgcagct
     2161 gcccccggac accgtctttc tgtgcatctt ccgcgaaccg gggcgcaccg ccgcgagcat
     2221 tctgcgcgaa cggcggcgca agtatccgga cctgcgtctc aggctcgaag acgctttcgg
     2281 agtctggaac gacatgtacc aacacgtgtt gcgcatccac cggcacagcg ggcgatggtt
     2341 gttcattcat tacgatcaga tgatcaacgg agccgcgata tcggccgtcg aaaagctgct
     2401 cggcgtgcat gtcgatgcat cgtttcccga tgtcgccttc gatcgcgctt ccccgatcaa
     2461 aacgcccgcg caagcacggg cgacctaccg cgagcta
//
LOCUS       DASUJG010000021         3214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_986251, whole genome shotgun sequence.
ACCESSION   DASUJG010000021 DASUJG010000000
VERSION     DASUJG010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986251"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="VFX38_00405"
     CDS             complement(<1..1005)
                     /locus_tag="VFX38_00405"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX5313356.1"
                     /translation="MLGSRRIVVTGLGTICPNGNRTAESWENVVAGRSGIGPLEHFDC
                     SQYPTRIIGAIKNFDPLRLVDVKELRKMDRFIQYALAAGIEAFEDSGLRIDEGNAGRC
                     GALIGSGIGGIGFLEACYDVYRNTGATRRMSPFTGPGSNISMAAGHLSMRLGLRGPSY
                     GLMAACSTSTHAIGLAARAIAYGDADVMLAGGTEAASTPTSIGGFCAARALSQRNDEP
                     EKASRPFDRDRDGFVISDGAAVVVLEEFEHARRRGARIYAELLGFGATSDAYHITSPR
                     EDGEGGVDAMRRALADAGLNPADIGYINAHGTATRVGDLAETIAMKKAFGELARRIPC
                     S"
     gene            complement(1148..2398)
                     /gene="fabF"
                     /locus_tag="VFX38_00410"
     CDS             complement(1148..2398)
                     /gene="fabF"
                     /locus_tag="VFX38_00410"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019018660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX5313357.1"
                     /translation="MKGQRRVVVTGMGVISPVGNSVTEAWASVLAGKSGIGPITQMDV
                     SGFTTRFGGVINDFDSERYLGPKDARKMDLFLQYGFAAGLDAFADSGLEVSEENADRL
                     GVLIGSGIGGLGMIESGYDAYLQGGGSRKMSPFVVPGSIINMISGHLSIRLGLKGPNL
                     AVATACTTSTHALGLAARLIAWDEADVMLAGGAERSTSPTGLGGFCSARALSQRNDEP
                     QKASRPWDRDRDGFVVADGAAVLVLEEYEHARRRGATMYAEILGFGMSGDAFHMTAPP
                     DDGDGARRAMIHALADAGLDSGKIGYINAHATSTKLGDLAETIAIKETFGEQAYRIPC
                     SSTKSMTGHLLGAAGAIEALFCVLALRDGVVPPTINLDNPDEGCDLDYVPQQARELEG
                     LRFAMSNSFGFGGTNGTLVFARLS"
     gene            complement(2436..2678)
                     /gene="acpP"
                     /locus_tag="VFX38_00415"
     CDS             complement(2436..2678)
                     /gene="acpP"
                     /locus_tag="VFX38_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX5313358.1"
                     /translation="MSSIEERVKKIVVEQLGVKEEDVKPEASFVDDLGADSLDTVELV
                     MALEEEFECEIPDEEAEKLTNVQQAVDYIKAHLPAE"
     gene            complement(2774..>3214)
                     /locus_tag="VFX38_00420"
     CDS             complement(2774..>3214)
                     /locus_tag="VFX38_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010639789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5313359.1"
                     /translation="KEEDWDAVLATDLTGVFRLTRRCLRDMIKRRRGRIIQIGSVVAA
                     SGNPGQTNYCAAKAGLEGFSRALAREVAQRGVTVNTVAPGFIETDMTRVLSESQREAL
                     ARQIPAGRLGSGTDVAAAVLYFASDGAAYVTGQTLHVNGGMYCG"
BASE COUNT          561 a         1060 c         1034 g          559 t
ORIGIN      
        1 cgagcaggga atccgccggg ccaattcccc gaacgccttt ttcatcgcga tcgtttcggc
       61 gagatccccg acgcgggtcg ccgttccatg ggcattgatg tagccgatgt cggccggatt
      121 cagcccggcg tcggcaagcg cccggcgcat cgcatccaca ccaccttcgc cgtcttcccg
      181 cggcgaggtg atgtgataag cgtcgctggt cgcgccgaat cccagaagtt ccgcatagat
      241 tctcgccccg cgccggcgcg cgtgctcgaa ttcctcgagt acgacgacgg cggcgccgtc
      301 ggagatcacg aacccgtcgc ggtcgcgatc gaagggccgg ctcgccttct ccggttcgtc
      361 gttgcgctgc gacagcgccc gcgccgcgca aaaaccgccg atcgaggtcg gcgtactggc
      421 ggcttcggtg ccgccggcaa gcatcacgtc ggcgtccccg taggcgatcg cgcgcgccgc
      481 aagtccgatt gcgtgggtcg aagtgctgca ggcggccatc aacccgtagc tcgggccccg
      541 cagtccgagg cgcatggaaa gatgtcccgc cgccatgctg atgttgctgc cgggtccggt
      601 gaacggcgac atgcgccgcg tggcgccggt attgcgatag acgtcgtagc aggcttcgag
      661 aaagccgatg ccgccgatgc ccgagccgat cagggcgccg caacgtccgg cgtttccctc
      721 gtcgatgcgc aagccggaat cctcgaaggc ttcgatgccg gcagccagcg catattggat
      781 gaagcgatcc atcttgcgca gttccttcac gtccaccagg cgcagcggat cgaagttctt
      841 gatcgcgcct attattcgcg ttggatattg cgaacagtcg aaatgctcga gcggcccgat
      901 tccgctccgt ccggcaacga cgttctccca gctttccgcg gtgcggtttc cgttcggaca
      961 gatcgtgccg agcccggtca cgacgatgcg gcgcgaacct aacatgccgg cgccgccgat
     1021 ggaggaaacg actgagagat catttggttg caggcgaatc gttcatgggt tccgaacctc
     1081 gcggggcgaa cgctttcggg gacgaactcg gagaaaagtt gcgacgatgg gccggtgacg
     1141 gaccgactca ggacagacgc gcgaatacga gcgtgccgtt ggttccgccg aatccgaagg
     1201 aattggacat cgcgaaacgc aggccctcga gctcgcgcgc ctgctgcgga acgtagtcga
     1261 gatcgcagcc ttcgtccgga ttgtcgaggt tgatcgtagg cggcaccact ccatcgcgga
     1321 gcgcgagcac gcagaagagc gcttcgatgg caccggccgc gccgagcaga tggccggtca
     1381 tcgacttggt cgaggagcag ggaatgcggt acgcctgctc gccgaaggtt tccttgatcg
     1441 cgatcgtctc ggcgagatcg ccgagcttgg tcgaggtcgc gtgcgcattg atgtaaccga
     1501 tcttgccgga atcgagcccg gcatcggcga gcgcatgaat catggcgcgg cgcgccccgt
     1561 cgccgtcatc ggggggcgcg gtcatgtgga acgcatcacc gctcatgccg aacccgagga
     1621 tttccgcgta catcgtcgcg ccacggcggc gcgcatgctc gtactcttcg agcacaagaa
     1681 cggccgcacc gtcggcgacc acgaagccgt cgcggtcgcg atcccaggga cggctcgcct
     1741 tttgcggctc gtcgttacgc tgcgacaagg cgcgcgccga gcagaagccg ccgagaccgg
     1801 tcggactggt cgagcgttcc gcgccgccgg cgagcatcac gtcggcctcg tcccaggcga
     1861 tcagacgtgc agccagcccg agcgcatggg tcgaggtcgt gcaggcggtc gcgaccgcga
     1921 gattcggacc cttgagtccg aggcgaatgg aaaggtgacc ggaaatcatg ttgatgatgc
     1981 tgccgggtac cacgaagggg gacatcttcc gcgacccccc gccctgcaga taggcgtcgt
     2041 agcccgattc gatcatgccg agcccgccga tgccggagcc gatcaggacg ccgagacgat
     2101 cggcgttttc ctccgatacc tcgaggcccg agtccgcgaa ggcatccaga cccgccgcga
     2161 atccgtactg caaaaagaga tccatcttgc gggcatcctt gggcccgaga tagcgctcgg
     2221 aatcgaaatc gttgatgacc ccgccgaagc gggtggtgaa tcccgacacg tccatctgcg
     2281 tgatcgggcc gatgccgctc ttcccggcga gcacgctggc ccaggcttcg gtaacggaat
     2341 tgccgaccgg agagatcaca cccattccgg tcacgaccac acgtcgctgg cccttcatcg
     2401 cgggcggtcc gggcctcgcg gcgaatgcga gggaatcact ccgccggcag gtgggccttg
     2461 atgtagtcca cggcctgctg gacgttggtc agcttctcgg cttcctcatc gggaatctcg
     2521 cactcgaact cttcttccag cgccatcacc agttccaccg tgtccaagga atcggcaccg
     2581 agatcgtcca cgaaggaggc ctccggcttg acgtcttctt ccttgacgcc cagctgctcg
     2641 acgacgatct tcttcacgcg ctcttcaatg ctgctcatcg gggcttatcc ctcctggtag
     2701 atgaaaaaac ccgtggccgg gatggcgccg ggagtgccta ttgtagagga atcccggccc
     2761 actcgccacc gtctcaaccg caatacatgc cgccattgac gtgcagggtc tgcccggtca
     2821 cataggcggc tccgtcggag gcgaaataga ggacggcggc ggcaacatcc gtccccgatc
     2881 cgaggcgacc ggccggaatc tggcgggcga gcgcctcgcg ctgactctcg gaaagtaccc
     2941 gcgtcatgtc ggtttcgatg aagccggggg cgacggtgtt cacggtcacg ccgcgttgcg
     3001 cgacctcacg ggcaagcgcc cgggagaacc cctcgagccc cgccttcgcc gcgcagtaat
     3061 tggtctgtcc gggattgccg ctcgccgcga ccaccgaacc gatctggatg atgcgtcccc
     3121 ggcggcgctt gatcatgtcg cgcagacacc ggcgggtcag gcggaatacg ccggtcagat
     3181 cggtcgcgag cacggcgtcc cagtcctctt cctt
//
LOCUS       DASUJG010000022         2731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_375551, whole genome shotgun sequence.
ACCESSION   DASUJG010000022 DASUJG010000000
VERSION     DASUJG010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375551"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VFX38_00425"
     CDS             complement(<1..194)
                     /locus_tag="VFX38_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5313360.1"
                     /translation="MSDRLKPQARDDRAPLSGLRVLDLSRVLAGPWCTQLLADLGAEV
                     IKVERPGGGDDTRSWGPPFAG"
     gene            323..967
                     /locus_tag="VFX38_00430"
     CDS             323..967
                     /locus_tag="VFX38_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313361.1"
                     /translation="MAAKSAIVEALLERHGRSFAEELGIPIAKNTPSPLFRLLCFALL
                     AGARISGAIAVSAARALADEGWTTAERLAGATWRERTDVLNHAGYARYDESTSRMLGE
                     TAELLVEEYGGDLRRLRERAGCDPAEERKLLKDCKGIGDVSANIFFREAQAVWGEWYP
                     FADKLALRAAGKLGLGSDAGTLARRVSRGDYPRLVAALVRCELAGDHQEVKAAA"
     gene            971..1345
                     /locus_tag="VFX38_00435"
     CDS             971..1345
                     /locus_tag="VFX38_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313362.1"
                     /translation="MRERFLHRKRWQAFKLPDEEKKGFKISAAEKKMAKQLVESTSGE
                     WNPESWHDDYRAALLEYIEQKARKGKVATEPAKPRKKAQVIDLTERLRKSIEQSSGGK
                     RGGRKKTAARKRSGAKRKAASR"
     gene            1372..1830
                     /locus_tag="VFX38_00440"
     CDS             1372..1830
                     /locus_tag="VFX38_00440"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX5313363.1"
                     /translation="MHDWRIQLILVGQIAAAMVLGGVVGWERELADKPAGFRTHMLVA
                     GASALFVGLAAMLTKDFQSILPPGGFRADPVRVLQSVVIGVSFLGAGTVFRSRDNRVG
                     GLTTAASILMAAGVGIACGLSRFILAVGATLLALLVLRLAHRVVGSRKKS"
     gene            2025..2699
                     /locus_tag="VFX38_00445"
     CDS             2025..2699
                     /locus_tag="VFX38_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HEX5313364.1"
                     /translation="MNKWHYLFSFSSAAVFAAALAAAPAFAAANPQTSPAYAQTSATQ
                     MNAVNHYNDNNGQQSSGQHKAMQHSDVQAAIAKFKQEAPNIQDYFDHAYGYAVYPSVG
                     KGGFVLGGAYGSGEVYKQGELVGYSTITKFDIGAQIGGQAFSEIIFFQDQQTFDHFTE
                     GHYQFAANLTAVGVKSGGIATNDYSNGVAVFARTKGGLMAGASIGGQKFSFTPLSQAG
                     SAATSN"
BASE COUNT          522 a          804 c          930 g          475 t
ORIGIN      
        1 cccgcgaagg gcgggcccca actgcgggtg tcgtcgccgc cgccggggcg ctcgaccttg
       61 atcacctcgg ctccgagatc ggcgagcagt tgcgtgcacc aggggccggc cagcacgcgc
      121 gagagatcga gcacgcgcag gccggataac ggagcccgat cgtcgcgggc ctgtggcttc
      181 agtcgatcgc tcatcgtccg gtctgcgcgt cgcaaaggcg cattgtcccg gtcggcgtgg
      241 tttcgtgcaa gtatgtcgct gcccgttgaa atggctgcgt ccgcggcggt attctgacgg
      301 tggcgatcgg aagagacgga atatggcggc gaagtcggcg attgtcgagg cccttctgga
      361 acgccacggg cgcagctttg ccgaggaact cggcattccg atcgcgaaga atacgccctc
      421 gccgctcttt cgcctccttt gctttgcctt gcttgccggc gcgcgcatca gcggcgcgat
      481 cgcggtgagc gcggcgcggg cgctcgccga cgagggttgg accacggccg aaaggctggc
      541 gggcgcgacg tggcgcgagc gcacggatgt gctcaatcat gcgggctatg cgcgttacga
      601 cgagagcacc tcgcgcatgc tcggcgaaac ggcagagttg ctggtggaag aatacggcgg
      661 ggatctgcgc agattgcgcg agcgcgccgg atgcgatccc gccgaggaac gcaagttgct
      721 caaggattgc aagggcatcg gcgacgtgag cgcgaatatt ttcttccgcg aagcgcaggc
      781 cgtctggggc gaatggtatc ccttcgccga caagctcgcg ctgcgcgcgg ccggcaagct
      841 aggactggga agcgatgccg ggacgctcgc gcgccgcgtg agtcgcggcg attacccgag
      901 gctggtcgca gccctggtcc gttgcgaact cgcaggagac caccaggaag tcaaagcggc
      961 ggcctgatcg atgcgggaac ggtttctgca tcgcaaacgc tggcaggcct tcaagcttcc
     1021 ggacgaggag aagaaaggtt tcaagatttc tgctgcggag aagaagatgg cgaaacagtt
     1081 ggtggaatcg acgagcgggg aatggaaccc ggagtcctgg cacgacgatt atcgcgcggc
     1141 gctcctcgag tacatcgagc agaaggcgcg caaggggaaa gtcgcgacgg aaccggccaa
     1201 accgcgcaaa aaggcgcagg tgatcgatct caccgagcga ctgcggaaaa gtatcgagca
     1261 gtcgagcgga ggcaaacgcg gcggccgcaa gaagacggct gcgcgcaagc gaagcggagc
     1321 gaaacgcaag gccgcttcgc gatgaaccgg tcgcggaacc ggggaggttg aatgcacgac
     1381 tggcgtatcc agctcatcct cgtcgggcaa attgccgccg ccatggtgct cggtggtgtc
     1441 gtcggctggg agcgcgaact cgccgacaag ccggcgggct ttcgcaccca catgctggtc
     1501 gcgggcgcct cggcgctttt tgtcggactg gccgcgatgc tgacgaaaga tttccagagc
     1561 atcctgccgc ccggcggttt tcgcgcggat ccggttcgtg tgctccagtc ggtcgtgatc
     1621 ggcgtgagtt ttctcggcgc gggcacggta tttcgcagcc gtgacaatcg cgtcggcggt
     1681 ttgacgaccg ccgcgagcat actcatggcc gcgggggtcg gcatcgcctg cgggctgagc
     1741 cgattcattc tcgccgtggg tgcgacgctg ctggcattgc tggtgctgcg gcttgcccat
     1801 cgggtggtag ggtcgagaaa aaaatcgtag cgcacaccga aacggtttcg gtgcgacatc
     1861 accgcatccg tgtgcgtctg cgcacgaaat cgaatcttcc aaagtgttac cgtgaattca
     1921 cgacgatctc gtcaattcgc tgagcggcgc tcgccatgat gcgccgatcc cgacgaagcg
     1981 caatgcttgc ggtttccatc cagtttctac agaggagtaa aggtatgaac aaatggcatt
     2041 atctgttttc gttttcctcc gcggccgtgt ttgccgcggc tctcgcggcg gcaccggcgt
     2101 tcgcggcggc caatccccag acctcgcccg catacgcgca gaccagtgcg acgcaaatga
     2161 acgcggtcaa tcattacaac gacaacaacg gtcagcagtc gagtgggcag cacaaggcga
     2221 tgcagcactc cgacgtgcag gcggcgatcg cgaagttcaa gcaagaggcc ccgaacatcc
     2281 aggattactt cgaccatgcc tacggctatg cggtgtatcc gagcgtcggc aaaggcggct
     2341 tcgttctcgg tggtgcctac ggcagcggcg aagtgtacaa gcaaggcgaa ctcgtcggct
     2401 attcgacgat caccaagttc gacatcggtg cccagatcgg cgggcaggcc ttcagtgaga
     2461 tcatattctt ccaggatcag cagactttcg atcatttcac cgaagggcac taccagttcg
     2521 ctgcgaatct gacggcggtc ggcgtaaaat ccggcggcat tgccaccaac gattacagca
     2581 acggcgtcgc agtttttgcc cgcacgaaag gcggtctgat ggcgggggcg agcatcggcg
     2641 ggcagaagtt cagcttcacg ccgctgtcgc aggcaggaag cgccgcgacc tcgaactgag
     2701 gggcgcgggg gcgtaccggc gagcccggga c
//
LOCUS       DASUJG010000023         2475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1598018, whole genome shotgun sequence.
ACCESSION   DASUJG010000023 DASUJG010000000
VERSION     DASUJG010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598018"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VFX38_00450"
     CDS             <1..636
                     /locus_tag="VFX38_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5313365.1"
                     /translation="PGFLETIIGEAQANGALVIFDEVMSGFRVGPRGAQGLYGLKPDL
                     TTLGKIIGGGLPAAAFGGRAGLMSQLAPDGPVYQAGTLSGNPLAMAAGLATLELTAAP
                     DFYERLAANTESLVEGLRTAAAESGVPVVVNQACGMFSLFFADRPMQNLDDVKRADTK
                     RYARWFHAMLDAGVYFAPSAFEAAFVSSAHGETEIAATLAAARTAFAGLDL"
     gene            complement(696..1571)
                     /gene="rsmI"
                     /locus_tag="VFX38_00455"
     CDS             complement(696..1571)
                     /gene="rsmI"
                     /locus_tag="VFX38_00455"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX5313366.1"
                     /translation="MSILPATLYVVATPLGNLADLGARAREILAAVDFIAAEDTRRTR
                     RLLESCAIRSRLTALHEHNEETRTPALVARLARGESAALVSDAGTPLVSDPGYRLVRA
                     AQDAGIAVRAVPGPCAAIAALSVAGLPSDRFAFEGFLPAKATARRGRLGALAKEPRTL
                     IFYEAPHRLIATLEDCVRAFGEAREAALARELTKVHESLHRRRLGELSQLVGSGAEPA
                     LGECVLLVAGARKDPGEETGGAPSADEVLEALAAEGLGAKTAAAVAHRLTGKSRRDLY
                     RRAIELARRVKTSKR"
     gene            1627..>2475
                     /locus_tag="VFX38_00460"
     CDS             1627..>2475
                     /locus_tag="VFX38_00460"
                     /inference="COORDINATES: protein motif:HMM:NF016251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HEX5313367.1"
                     /translation="MQARSAAATLGAFALALLVAGCATGPSAPRASSGGKAATPLEAR
                     GRYEAAADAWLKAAAGLQGSRQAEARLRAAEDYEKAKKPQAAWRAIEPMAISDLPPKE
                     RFDGALTKARLALAVHRPQDALVALAAAPAPPGRAEQLQFLALKGQVEFAADDPRTGL
                     ETLVERGTLVTSQDQVLANDRLIWSELGHASNLPEPQGLSPTAVGWVKLARIQRNAWL
                     APGEFTQQLQAWRIAYPNHPADYGLLAEIQSRAASFGSYPAQVALLLPLSGPYASQGA
                     AVEHGLL"
BASE COUNT          367 a          842 c          855 g          411 t
ORIGIN      
        1 ccgggattcc tcgaaacgat catcggggag gcgcaggcga acggggcgct cgtgatattc
       61 gatgaagtga tgagcgggtt tcgcgtcggg ccgagaggtg cgcagggctt gtacggattg
      121 aagcccgatc tcaccacgct cgggaaaatc atcggcggcg gtctaccggc cgcggccttc
      181 ggcggtcgtg ccgggctgat gtctcagctt gcgcccgacg gacccgtgta tcaggccggc
      241 acgctttcgg gaaatccgct ggcaatggcc gcggggctcg cgacgctcga actcacggcc
      301 gcgccggact tctacgagcg gctcgcggcc aacaccgagt cgctcgtcga aggcttgcgg
      361 accgcggccg cggagtcggg cgttcccgtc gtcgtgaatc aggcctgcgg aatgttctcg
      421 ctctttttcg ccgatcggcc gatgcagaac ctcgatgacg tcaaacgtgc cgatacgaaa
      481 cgctacgcgc gctggtttca cgccatgctc gatgccggcg tttactttgc tccttccgca
      541 ttcgaggcgg cgttcgtgtc ttcggcgcac ggcgaaacgg agatcgccgc gacgctcgcc
      601 gccgcccgta ctgcatttgc aggtctcgac ttgtaggagc gggccatgcc cgcgatctgc
      661 gcggcgatgg attctgtcgc gggcatggcc cgctcctacc ttttgcttgt tttgacgcga
      721 cgggcgagtt cgatggcgcg gcggtagagg tcgcgccgtg attttcccgt caggcgatgc
      781 gcgaccgcgg cggcggtctt cgcgcccagg ccttccgcgg cgagcgcctc gaggacttcg
      841 tcggcacttg gcgcgccgcc ggtttcttcg ccggggtctt tgcgcgcgcc ggcgacgagc
      901 agcacgcatt ctccgagcgc cggctccgct ccgctgccga ccaattgcga gagttccccg
      961 agcctgcgac ggtgcagact ttcgtgaact ttcgtaagtt cgcgggcgag cgccgcctcg
     1021 cgcgcttcgc cgaaagcgcg cacgcaatct tcgagagtcg cgatcagccg gtgcggcgcc
     1081 tcgtagaaaa tgagcgtgcg cggctccttc gcgagagcgc cgagccggcc ccgccttgcc
     1141 gtggctttcg ccggcagaaa gccttcgaag gcaaagcgat cgctcggaag ccccgcgacg
     1201 gagagtgccg cgatcgcggc gcaggggccg ggcacggcac gcacggcaat gcccgcatcc
     1261 tgggccgcgc gtaccagacg atatcccgga tcgctcacga gcggcgtgcc ggcgtccgac
     1321 accagcgccg cactttcccc gcgcgcgagc cgtgcaacga gcgccggcgt acgcgtctct
     1381 tcgttgtgct cgtgaagcgc ggtgagacgg ctgcggatgg cgcagctctc gagcagccgg
     1441 cgcgtgcgcc gcgtgtcttc ggcggcgatg aaatccacgg ccgcgagtat ctcgcgcgcg
     1501 cgggcgccga gatcggcgag gttgccgagc ggagtcgcga cgacgtagag cgtggcgggt
     1561 aaaattgaca tggacggctc tcgcgggaat actggcatct cctatcctcc gatgcattcg
     1621 cgatccatgc aagcacgaag cgccgccgcg accctcggcg catttgccct ggccttgctc
     1681 gtggccggct gcgccaccgg gccgtcggcg ccgcgggcgt cctccggggg caaggccgcg
     1741 accccgctgg aggcccgcgg gcgctatgag gccgcggccg acgcctggct caaggccgcc
     1801 gcggggttgc agggaagccg gcaggccgag gctcgcctgc gggcggccga ggattacgaa
     1861 aaggcgaaaa aaccgcaggc cgcctggcgg gcgatcgagc cgatggcgat ttccgatctc
     1921 ccgcccaagg agcgcttcga cggcgcgctg accaaggccc gcctcgcgct cgcggtgcat
     1981 cgcccgcagg acgccctcgt ggcgctcgcg gccgcgccgg ctccgcccgg ccgcgccgaa
     2041 caactgcaat ttctggcact caagggccag gtcgagttcg ccgcggacga tccgcggacc
     2101 ggtctggaaa ctctggtcga acgcggaacg ctcgtcacca gccaggatca ggttctcgcg
     2161 aacgaccggc tgatctggag cgagctcggt cacgcttcga atctgccgga accgcagggc
     2221 ctgtcgccga ccgccgtggg ttgggtcaag ctcgcccgca ttcagcgcaa tgcctggctg
     2281 gcgcccggcg aattcacgca acagcttcag gcctggcgca tcgcctatcc gaaccatccc
     2341 gccgactacg gcctgcttgc ggagattcag tcgcgcgcgg cgagcttcgg aagctatccg
     2401 gcgcaggtcg ccttgcttct tcccttgagc ggaccgtacg cgagtcaggg tgcggccgtc
     2461 gaacatggat tgctc
//
LOCUS       DASUJG010000024         2255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_375642, whole genome shotgun sequence.
ACCESSION   DASUJG010000024 DASUJG010000000
VERSION     DASUJG010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375642"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="VFX38_00465"
     CDS             <1..1023
                     /locus_tag="VFX38_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEX5313368.1"
                     /translation="LARPLPHQYEFARLNLTYAVLSKRKLLRLVDEGHVDGWDDPRMP
                     TLVGARRRGYTPSGFRAFNERIGVSKSDSWIDMSVLEDAMRDDLNAAAVRRIAVLDPV
                     RLVIDNYPDGRSEDMSVPNHPQKPEVGRRMLPFTRELWIERDDFSETPPKGYFRLSPG
                     AEVRLRYAYIVRCTGVEKDADGRVAVVHCTYDAATRSGTAGADARKVKGNIHWLSTAH
                     ALPAEVRLYDRLFRVPFPGARDGGMPEGETAGAADPLPAHASNAGVDDEPADERDFID
                     DLNPDSKRVIRAFVEPSLALATSDERIQFERHGYFVADSGDHAPGRPVFNRTVTLRDS
                     SAKPSR"
     gene            1176..2045
                     /gene="htpX"
                     /locus_tag="VFX38_00470"
     CDS             1176..2045
                     /gene="htpX"
                     /locus_tag="VFX38_00470"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEX5313369.1"
                     /translation="MKRIILFVATNLAVLVVLSIVLKIFGFDRALAAQGLQYGQLLVY
                     SLVVGFTGAIISLLMSKSIAKWTTGAQMIDTPRNEAEAWLLDTVRKLANQVGINMPEV
                     GIYEGPANAFATGAFKNSAFVAVSSGLLQSMNHEEVEAVLGHELSHVANGDMVTLTLI
                     QGVVNTFVVFLSRIVGSIVDRAVFRTERGTGPGYFITVIVCQIVFGILASMIVAWFSR
                     WREFRADAGSARLLGAQPMIHALQRLAGIEAGGLPQAMNAFGIAGSGGFMALFASHPP
                     IEQRIAALQATGR"
     gene            2050..>2255
                     /locus_tag="VFX38_00475"
     CDS             2050..>2255
                     /locus_tag="VFX38_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313370.1"
                     /translation="MRYKIEVQDDTGIWSDVRGDDGRILVFDDENAARAALAERFPVL
                     VQMEKYAGGKRTRVIRILEDEDDWP"
BASE COUNT          366 a          756 c          755 g          378 t
ORIGIN      
        1 ctcgcgcggc cgctgccgca tcaatacgaa ttcgcgcgcc tcaacctgac ctacgcggtg
       61 ctgtcgaagc gcaagctgct gcggctcgtc gacgaaggcc atgtcgacgg ctgggacgat
      121 ccgcgcatgc cgacgctcgt cggtgcgcgc cggcgcggct acacgccgtc cggcttccgc
      181 gcgttcaacg agcggatcgg cgtgtcgaag tccgattcat ggatcgacat gagcgtgctc
      241 gaggacgcga tgcgcgacga cctgaacgcg gccgcggtgc gccgcatcgc ggtgctcgac
      301 ccggtcaggc tcgtcatcga caactacccg gacgggcgga gcgaggacat gagcgtcccc
      361 aatcacccgc agaagcccga ggtcggacgg cgcatgctgc cgttcacgcg cgagctctgg
      421 atcgagcgtg acgatttctc ggaaacgccg ccgaagggct atttccggct ctcgccgggc
      481 gccgaagtgc gcctgcgcta cgcgtacatc gttcgttgca ccggcgtcga gaaggatgcc
      541 gacggccgcg tggcagtcgt tcactgcacc tacgacgccg cgacgcgcag cggcactgcc
      601 ggtgccgatg cacgcaaggt gaagggcaac atccactggc tgtcgacggc gcatgcgctg
      661 cccgccgagg tacgcctcta cgaccggctc tttcgcgtgc cgtttcccgg cgcgcgcgac
      721 ggcggcatgc cggaaggcga gacggccggc gccgccgatc cgttgccggc tcacgcgtcg
      781 aacgccggcg tcgacgacga gccggcagac gagcgcgatt tcatcgacga cctcaatccc
      841 gactcgaagc gcgtcatccg tgcgttcgtc gagccgtcgc tcgcgctcgc gacatcggac
      901 gagcgcatcc agttcgagcg acacggttac ttcgtcgccg actcgggcga tcatgcgccc
      961 ggccgtccgg tcttcaatcg cacggtgacg ctgcgcgatt cgtcggcgaa gccttcgcgt
     1021 tgagcatcga tcgcgcgcgt ggccggcctt gtggcgggcg ggacgcgcgc taatattcga
     1081 gaaacctccg aacgagtccc gcgcggacca ttcgaaggtt cgacagcgtt cgcgagcgcg
     1141 caatccgcgc gcttttgcga atcgcggagg ccaccatgaa gcggatcatc ctgttcgtcg
     1201 ccacgaatct cgcggtcctc gtcgtcctgt cgatcgtgct gaagatcttc gggttcgacc
     1261 gcgcgctcgc ggcgcagggt ctccagtacg ggcagcttct cgtctactcg ctcgtcgtcg
     1321 gcttcaccgg cgccatcatt tcgctgctga tgtcgaagtc gatcgcgaag tggacgaccg
     1381 gcgcgcagat gatcgacacg ccgcgcaacg aagccgaagc gtggctgctc gacaccgtgc
     1441 gcaagctcgc gaaccaggtt ggcatcaaca tgcccgaggt cggcatctac gaaggtcccg
     1501 cgaacgcgtt cgcaacgggc gccttcaaga actcggcatt cgtcgccgtg tcgagcggac
     1561 tgctgcagtc gatgaaccac gaggaggtcg aggccgttct cgggcacgag ctctcgcacg
     1621 tggcgaacgg cgacatggtg acgctgacgc tgatccaggg tgtcgtcaac acgttcgtcg
     1681 tgttcctgtc gcgcatcgtc ggctcgatcg tcgaccgcgc cgtgttccgt accgagcgcg
     1741 gcacgggccc gggctacttc atcaccgtca tcgtctgcca gatcgtgttc ggcatactcg
     1801 cgagcatgat cgtcgcgtgg ttctcgcgct ggcgcgagtt tcgggccgac gccggcagcg
     1861 cgcggttgct gggagcgcag ccgatgatcc atgcgctgca gcgtctcgcc ggcatcgaag
     1921 cgggggggct gccgcaggcg atgaacgcgt tcggcatcgc gggcagcggc ggcttcatgg
     1981 cgctcttcgc gtcgcatccg ccgatcgagc agcggatcgc cgcgctgcaa gccacaggac
     2041 gttgacgaca tgcgctacaa gatcgaggtg caggatgaca cgggcatctg gagcgacgtg
     2101 cgcggcgacg acggacggat cctcgtcttc gacgacgaga acgccgcgcg cgccgcgctc
     2161 gccgagcgct ttccggttct cgtgcagatg gagaaatacg cgggcggcaa gcgcacccgc
     2221 gtcattcgca ttctcgaaga cgaggacgac tggcc
//
LOCUS       DASUJG010000025         2522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1598820, whole genome shotgun sequence.
ACCESSION   DASUJG010000025 DASUJG010000000
VERSION     DASUJG010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598820"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VFX38_00480"
     CDS             <1..865
                     /locus_tag="VFX38_00480"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX5313371.1"
                     /translation="DRRLSGKPRMNELAWVDGRILPLAEARIALDSPGFLLGDGVFET
                     LRTRRGRVFRWDLHAARFAKGLAALDIAPAALPIAERAARELAGAAANCEDDLSLRVQ
                     VSRDASGIGEVSALIRPLPPRVERLYREGARLAIATSRRDPADPLAGVKSLSYLPQVL
                     ARRAARARGFDDALILNTPGRVCEAAHANVIARSGRRLYAPGREEGALDGVTRRVLLD
                     DIAAGEDELQFRLERSELEAAEELVLTSTLAGVIPVASIEGVAKSYAGSAGELARGLA
                     RRYEVLLESGV"
     gene            complement(835..1338)
                     /locus_tag="VFX38_00485"
     CDS             complement(835..1338)
                     /locus_tag="VFX38_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313372.1"
                     /translation="MRNHHLRALRAVVCGGLGAGTLDIFIAAWIGRVSPLVVMQAIAS
                     GLLGSAALRGGASAAALGFLSQWILSLIIAAVYVLSAQRIRLLVRYWFVTGIAYGVVI
                     FFVMNYVVMPLSAAPFGPHFTARSFVLNLLAMLLFGLIVAGFAQLFLGRRRDDARVQT
                     PLSSKTS"
     gene            complement(1351..2271)
                     /locus_tag="VFX38_00490"
     CDS             complement(1351..2271)
                     /locus_tag="VFX38_00490"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313373.1"
                     /translation="MNGDHCPSTLSPTESAGLRPAGSLLCADTGLALVRIAGRDATAF
                     LDAQCTAAIGEDCEAVTAAALADAKGRVLAVFLALPRGDDEWLLAVTASEEEWLREHL
                     ARFVFRSRVAIAPATDLRPIGLLGPDADRALAAAGLPAPAESRLVRAGPLLVCGIGSG
                     RGLVIGEDMALADAARALAARLDTGDLAAWTRARLLTNEVAIVAATRARFLPQMLGLV
                     ERGAVSFRKGCYPGQEIIARTQHLGRVKRGLALFRGEIAPVPGAAQETERGPAEVLDG
                     VALPAGGFLIQAVAPLAGDEDSQPYNGVRQ"
BASE COUNT          424 a          840 c          893 g          365 t
ORIGIN      
        1 tgatcgccgc ctttctggaa agccgcggat gaacgaactc gcctgggtcg acggacgcat
       61 tctgccgctc gccgaggcga ggatcgcgct cgacagcccg ggttttcttc tgggcgacgg
      121 cgttttcgaa acgctgcgca cgaggcgcgg gcgagtgttt cgctgggatc ttcacgcggc
      181 gcgcttcgcg aagggtctcg ccgcgctcga catcgcgccc gctgcgctcc cgattgccga
      241 gcgtgcggca cgggaacttg ccggagccgc cgcgaattgc gaggatgacc tttcgctgcg
      301 ggtgcaagtg tcgcgcgacg cgagcggcat tggcgaggtg agcgcgctga tccgtccgtt
      361 gccgccacgc gtcgaacgac tgtatcgcga gggtgcgcgg ctcgcgattg ccacttcgcg
      421 ccgcgatccc gccgacccgc tcgcgggggt gaagtcgctg agttatcttc cacaggtcct
      481 ggcgcggcgc gcggcacgag cgcgcggttt cgatgacgcg ttgatcctga acaccccggg
      541 ccgcgtctgc gaggccgcgc acgccaatgt catcgcccgg agcggtcgcc gcctctatgc
      601 gccgggtcgg gaggagggag cgctcgacgg ggtcacccgg cgtgtgctgc tcgacgacat
      661 cgccgcgggc gaagacgagt tgcaattccg tctcgagcgc agcgaactcg aagcagccga
      721 ggagctggtt ctcacgagca cgctggccgg cgtgattccg gtcgcttcga tcgagggcgt
      781 ggcgaagagc tacgccggat ctgcggggga actcgcgcga ggtctcgcgc gccgctacga
      841 agtcttgctc gaaagcggag tctgaacgcg cgcatcgtcg cgccggcgcc cgagaaacaa
      901 ctgggcgaaa ccggcgacga tcaggccgaa gagcagcatc gcgagcaggt tgaggacgaa
      961 gctgcgcgcg gtgaaatgcg ggccgaaggg tgcggccgaa agcggcatca ccacgtagtt
     1021 catgacgaag aagatcacga cgccgtaggc gatgccggtc acgaaccagt agcggacgag
     1081 cagccggatc cgctgcgcgg agaggacgta cacggcggcg atgatcagcg acaggatcca
     1141 ttgcgacagg aagccgagcg ccgccgccga ggcgccgccg cgcaacgccg cgctgccgag
     1201 gagcccgctc gcgatcgcct gcatgacgac gagcgggctc acccggccga tccacgcggc
     1261 gatgaaaatg tcgagcgtgc ccgcgccgag tccgccgcag acgacggcgc gaagcgcgcg
     1321 aaggtggtgg ttccgcatcg gttcgcttcg ttattggcga acgccattgt atggttgcga
     1381 atcttcgtcg cccgcgagcg gtgctaccgc ctggatcaga aagccgcccg cgggaagggc
     1441 gacgccgtcg agtacctccg ccgggccgcg ctccgtctcc tgcgccgccc ccggcaccgg
     1501 tgcgatctcc ccgcgaaaca gggcgagccc gcgcttgacg cggccgagat gctgcgtgcg
     1561 ggcgatgatc tcctgcccgg gatagcaacc cttgcgaaag ctcaccgcgc cgcgctcgac
     1621 gagccccagc atctgcggca ggaagcgcgc gcgggtggcg gcgacgatcg cgacttcgtt
     1681 cgtgagcagc cgtgcccgcg tccacgccgc gaggtcgccg gtgtcgagcc gtgccgccag
     1741 ggcccgtgcc gcgtcggcga gcgccatgtc ctcgccgatg accagtccgc ggccactgcc
     1801 gatgccgcag acgagaagcg ggcccgcacg aacgagacgg ctttcggctg gcgcgggcaa
     1861 gcccgccgcg gcgagcgccc gatcggcatc gggaccgagc agcccgatgg ggcgcaggtc
     1921 cgtcgccggg gcgatcgcga cccgggagcg aaacacgaag cgagcgagat gctcccggag
     1981 ccattcttct tcgctcgcgg ttacggcgag cagccattcg tcgtcgcccc tgggcagcgc
     2041 caagaacaca gccagcacgc ggccctttgc atccgccagc gccgccgccg tcacggcttc
     2101 gcaatcctcg ccgatggcgg ccgtgcactg cgcatcgaga aaagcggtgg cgtcgcgccc
     2161 ggcgatacga acgagcgcga gccccgtgtc cgcacacagg aggctgccgg cgggacgaag
     2221 gcctgcgctt tccgtcgggc tcagggtgct cgggcagtgg tccccgttca tcgacatatc
     2281 gtagcgtcgc caggttcgca aaagggcaat tcgcggtccc ggcccgcctt gagcaggccg
     2341 atggcccgat gctagaatcg aattggatga caaggtctac gagaaaacgt ctccgcgggg
     2401 ttctccggaa ttcttcggag agacgggccg gcgcgctggt cggcgcttgc ccggcggaac
     2461 ttgccgatgg agtttccgcg aaacaagcaa ttcaagcaag aggatgaaca acatggctac
     2521 ca
//
LOCUS       DASUJG010000026         2397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_376961, whole genome shotgun sequence.
ACCESSION   DASUJG010000026 DASUJG010000000
VERSION     DASUJG010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376961"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VFX38_00495"
     CDS             <1..679
                     /locus_tag="VFX38_00495"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HEX5313374.1"
                     /translation="AGFYSIIAAMSGLFTAAAIAIFIALVLDGLDGRVARFTGTTSAF
                     GAQYDSLSDLVAFGLAPALLVYRFALSGLADYGWVWAKIGWLAAFFYAVATALRLARF
                     NSRAGIPKRYFQGLPCPSAAALIAGSIWFAQSLGVSSHALAIPALAFTVFAGVLMVSD
                     LGYYSFKELRLDRRLAFPSVLAVPLLFILVTLSPPGVLFGAFLLYALSAPVWWLIRWY
                     RRYRRHA"
     gene            681..1436
                     /locus_tag="VFX38_00500"
     CDS             681..1436
                     /locus_tag="VFX38_00500"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018993147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX5313375.1"
                     /translation="MDVRREAVLGTMGFTRWVQRNAPEPRQTENVALPADRGRRLPTG
                     ARVTPIPVVRVQPPAREAGTQPAEWERLESEVSTCTRCALAAGRTRTVFGLGARDARW
                     MFVGEGPGAEEDRRGEPFVGRAGQLLDAMLAAIGLARRQVYIANVVKCRPPGNRDPLP
                     EEADACWPYLRSQIAWVNPALVIALGAVAARRLLGVEDSLGRLRGRVHEGPDGHALLV
                     TYHPAYLLRNPAAKREAWQDLKLALATASPLLP"
     gene            1433..1903
                     /gene="rimI"
                     /locus_tag="VFX38_00505"
     CDS             1433..1903
                     /gene="rimI"
                     /locus_tag="VFX38_00505"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX5313376.1"
                     /translation="MNAVLAIAAPLLRPLVPGDLERLAAIEARAYRSGWTGGIFADCL
                     RVGYSCWGMEAAGVLAGYGIISIAAGESHLLNIAVDPTQQRGGLGGLMLEHLLGIARR
                     WRVEHMFLEVRPSNVAARRLYERAGFAEFGRRRGYYPGGNASEREDALILRLQL"
     gene            1935..>2397
                     /locus_tag="VFX38_00510"
     CDS             1935..>2397
                     /locus_tag="VFX38_00510"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX5313377.1"
                     /translation="MTIRLPGCAMAGLLACVAAGTAYALEAPPAGVGPSGSGDCSRHI
                     AITLDDLPFADQRLSMAEQRAGTEALLQALKLHRVPAIGFVNEDKLLVDGEVSERIGL
                     LRNWLNAGMTLGNHGFGHLDLQTTPLPEAEAAVLQGEVVTRWLMKQYGEAPR"
BASE COUNT          302 a          811 c          815 g          469 t
ORIGIN      
        1 cgcgggcttt tactcgatca tcgccgccat gagcggcctg ttcaccgccg cggcgatcgc
       61 aatcttcatt gcgctcgttc ttgacgggct cgacgggcgg gtcgcgcgct tcaccggcac
      121 cacctcggcc ttcggcgcgc aatacgacag cctttccgat ctcgtcgcct tcgggctcgc
      181 gcccgcgttg ctcgtgtatc gcttcgcgct ttcggggctc gccgattacg gctgggtctg
      241 ggccaagatc ggctggctcg ccgcattctt ctacgcggtc gcgacggcgc tgcgcctcgc
      301 gcgcttcaac agccgcgccg ggattcccaa gcgctatttt cagggtttgc cctgtccctc
      361 tgcggccgcc ctgatcgccg ggtctatctg gtttgcgcag agcctcggcg tttcctcgca
      421 tgcgctcgcg attccggcgc tggcgtttac cgtgttcgcg ggcgtgctga tggtgtccga
      481 tctcggttac tacagcttca aggaacttcg tctcgatcgg cgcctcgcct tcccctcggt
      541 gctcgcagta ccgctgctgt tcattctggt cacgctctcg ccaccgggag tgctgttcgg
      601 agcatttctg ctgtacgcgc tttcggctcc cgtgtggtgg ctgatccgct ggtaccggcg
      661 ttatcggcgt cacgcctgac atggacgtgc gccgcgaagc cgtgcttggc accatgggtt
      721 tcacgcggtg ggtgcaacgc aacgcgccgg agccgcggca gacggagaac gttgcgctac
      781 ccgccgatcg cggccggcgc ttgccgaccg gcgcgagggt aacgcccatt cccgtcgtcc
      841 gcgtgcagcc tcccgctcgc gaagcgggga cgcagccggc tgaatgggaa cgtctcgagt
      901 ccgaggtctc gacttgcacg cgctgcgcgc tcgcggcggg gcgaacccgc accgtgttcg
      961 ggttgggggc gcgcgacgcg cgctggatgt tcgtcggcga gggacccggc gccgaggaag
     1021 accgccgcgg cgagcccttc gtcggcaggg cggggcagct cctcgatgcg atgcttgcgg
     1081 ccatcggtct cgcccgccgg caggtgtaca tcgcgaacgt ggtgaagtgc cgcccgcccg
     1141 gcaatcgcga tccgctcccc gaggaggccg atgcctgctg gccttatctt cgcagtcaga
     1201 ttgcctgggt gaatcccgcg ctcgtgattg ctctgggcgc cgtcgcggcg cgacgcctcc
     1261 tcggagtgga ggattcgctc gggcggctgc gcgggcgcgt ccacgagggg ccggacggac
     1321 atgcgctcct cgtcacgtat catccggcct atctgcttcg caatccggcc gcgaagcgcg
     1381 aggcctggca ggatctcaag cttgccctag ccaccgcgag tccgcttttg ccgtgaacgc
     1441 cgtcctcgcc atcgccgcgc ctctgctgcg cccgctcgtg ccgggcgacc tcgagcgcct
     1501 cgccgcgatc gaggcgcggg cgtatcgcag cggctggacg ggcggcattt tcgcggattg
     1561 cctgcgggtc ggctactcgt gctggggaat ggaggccgcg ggcgtgctcg ccggctacgg
     1621 catcatctcg atcgcggccg gtgaatccca tctgctcaat atcgccgtgg atccgacgca
     1681 gcaacgcggc ggcctcggcg ggctcatgct cgaacatctg ctcggcattg cccggcgctg
     1741 gcgggtggag catatgtttc tcgaagtgcg cccgtcgaac gtcgcggcgc gccggctcta
     1801 cgagcgcgcg ggtttcgcgg aatttgggcg ccgccgcggc tactatcccg gcgggaacgc
     1861 aagcgagcgc gaggacgcac tgattcttcg tttgcaattg tgagggtggc acttttccgt
     1921 cccggcttgt cgccatgacg attcgcctcc ccggctgcgc gatggccggc cttctcgcct
     1981 gcgtggccgc gggcactgcc tatgccctcg aggctccgcc cgccggcgtc ggcccttccg
     2041 gttccgggga ttgcagccgg cacatcgcga ttaccctgga cgatctcccg ttcgcggacc
     2101 agaggctttc catggcggag caacgtgccg ggaccgaagc gctgttgcag gcgttgaaat
     2161 tacaccgcgt gcccgccatc ggcttcgtga acgaggacaa actccttgtc gacggcgaag
     2221 tgagcgagcg catcggattg ttgcggaact ggttgaatgc cggcatgacc ctgggcaatc
     2281 atggtttcgg tcatctggat ctgcagacga ccccgctgcc cgaggcggag gcggcggttt
     2341 tgcaagggga ggtggtgacc cgctggctga tgaagcagta cggcgaggcg ccccgct
//
LOCUS       DASUJG010000027         2383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_988399, whole genome shotgun sequence.
ACCESSION   DASUJG010000027 DASUJG010000000
VERSION     DASUJG010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988399"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1290
                     /locus_tag="VFX38_00515"
     CDS             <1..1290
                     /locus_tag="VFX38_00515"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313378.1"
                     /translation="WTQVAELGASDGAAHDSFGWSVALSGATALVGSPAAEVNGVSGE
                     GAAYVFTQTGDTWSEAQKLTANNSAGVIHRFGGALALEGSTALIGAPWSAVADNGEFG
                     AAYVFGESGGNWVQTQELVPTDGVTGVSFGDAVALEGASMLIGACDETQTYVGAADVF
                     VNQNGVWSQVQRLAASNGFAWDQFGCAVALDDGTALVGAPGVYGEDLDMGAAYFYGRC
                     ELELALSAPQTVSQGQQYISEAIATNNASAASPAVAVTATVPAAASFVSVIASQGSCS
                     EHSGVVNCDFGAIDGNGGTATLNLTLEATGASGSTIENTASIAKATPALTASAATEIT
                     GTQSCPDGYSIFTGTLPPNGRTRGPSYEAPAGEENAILYAPAGFRLYGRVDTAHGRRQ
                     QSFPGNEVHRWGPAGTYAWGIRAGEAGGAYTICTLHP"
     gene            1792..>2383
                     /locus_tag="VFX38_00520"
     CDS             1792..>2383
                     /locus_tag="VFX38_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313379.1"
                     /translation="MNQYSCKTLLFLLAFGGALAPAMAAVPAQSAAIAPNASRHQTIP
                     AALRPAFYQALAKDASRAYVIGKDGCAALPKQQLTACFDRRGAHFTGAGAPLVLQLVA
                     WGRQSAGDANGDKLVATKPVMPTIRGNEVRYAHGDLRAWWRVLPVGFEQGFTIAKRPA
                     GNGRLVLMLAKSRDTAGAPSIRHSRADGNPAKNTLAW"
BASE COUNT          415 a          774 c          802 g          392 t
ORIGIN      
        1 tggactcagg tggcggaact cggcgcaagc gacggcgccg cgcacgacag cttcggctgg
       61 tcggttgcgc tctccggcgc aaccgctctc gtagggtcgc ccgccgccga agtcaacggc
      121 gtttcgggcg aaggagcggc atacgtcttc acgcaaaccg gagacacctg gagcgaggcg
      181 cagaagctca ccgcgaacaa cagcgcgggc gtgattcatc gcttcggcgg cgctctcgct
      241 ctcgaaggct ccaccgccct gatcggcgcg ccctggtccg cggtcgccga caacggggag
      301 ttcggcgcgg cctatgtctt cggcgagtcc ggcggcaact gggtacagac gcaggaattg
      361 gtcccgaccg acggcgtaac gggagtctcc ttcggcgacg ccgtcgcact cgagggggct
      421 tcgatgctca tcggcgcctg cgatgaaacg cagacgtacg tgggagcggc agacgtgttc
      481 gtgaaccaga atggcgtgtg gagccaggtt caacggctcg cggcgagcaa tggtttcgcg
      541 tgggatcagt tcggctgcgc cgtcgccctc gacgacggca cggccctcgt cggagctccg
      601 ggcgtgtacg gcgaagatct ggacatgggt gccgcctatt tctacggcag gtgcgagctg
      661 gagcttgcgt tgagtgcgcc tcagacggtg agtcagggac agcaatacat cagcgaagcg
      721 atcgccacca acaatgccag tgcggcatcg ccggcggtgg ccgtcaccgc aacggtgccg
      781 gcggcagcga gcttcgtgtc cgtgatcgcg agtcagggca gttgcagcga acactcgggc
      841 gtcgtgaatt gcgacttcgg tgcgattgac ggcaacggcg gcacggcaac gttgaacttg
      901 acgctcgagg caaccggcgc ctccggcagc accatcgaaa acacggcaag catcgccaag
      961 gcgacgccgg ccctgacggc aagtgccgcg accgagatta cgggtaccca gagctgtccc
     1021 gacggctaca gcatctttac cggcacgctg ccgccgaacg ggcgaacgcg cgggccgagt
     1081 tacgaagcac cggcgggcga ggagaacgca attctctacg cgcccgcggg cttccggctt
     1141 tacgggcgcg tggataccgc gcacggccgc cggcagcaga gctttccggg caacgaggtg
     1201 catcgctggg gtcccgcggg cacttacgcg tgggggataa gagccggcga agccggcggc
     1261 gcctacacga tttgcacgct gcatccctga tcggaaatgc cgacgcaaaa ccgggcgacg
     1321 acctcgccgt cccgcgcatt cgcggctgat gtccgcccgg cgtgcattcg gcgtccgagg
     1381 aatgaagaca tccccccgcg cggacgtggc ccctttcgct tgcgaagggg gcgaagacag
     1441 cgctccttcg tttgcgaaaa gcccggaacg tttctctttg ccggagaagg ccctcgcaat
     1501 aattcccttg cgatcgcgaa gacctcggcg gtatttcccc cttcgcttgc gaagggggat
     1561 tcagagggat gttcgaagga cgcggggcgc tgcccgtcgc ggccctacgg cgatgcttgc
     1621 cacggccagt ttctcgggcg tatagttcct ctcagcacgc gttggcgtag cgacccggcc
     1681 gacgaacggc cgccacgata acaaccgttc gccgggccct tccgatatct gcgcgcgtgc
     1741 cggtccggat cgccgcgatc cgggagaatt cttctgcgag gggcaaattc gatgaaccag
     1801 tattcctgca agactctgct gttcctgctc gcgttcggcg gagcgctcgc accggcgatg
     1861 gcggcagtgc cggcccaatc tgccgcgatc gcgccgaacg cttcccggca tcagacgatt
     1921 cccgcggcat tgcgcccggc cttttatcag gcgctcgcca aggacgcgag ccgggcctac
     1981 gtgatcggca aggacggctg tgccgctttg ccgaagcagc agctgaccgc ctgcttcgat
     2041 cgtcgtggag cgcacttcac gggagcgggc gcgccgctcg tgctgcaact cgtcgcctgg
     2101 ggacgccaat ccgccggcga tgcgaatggc gacaagctcg tcgcaacaaa accggtgatg
     2161 ccaacgattc gcggcaacga agtccgctat gcccatggcg atcttcgcgc gtggtggcgg
     2221 gtgttgccgg tcggcttcga gcagggtttc accatcgcca aacgtccggc gggaaacggc
     2281 cgacttgttc tgatgctggc caagagccgg gacactgccg gtgctccctc gatccgtcat
     2341 tcccgcgccg acgggaatcc agcgaagaac accctcgcat ggg
//
LOCUS       DASUJG010000028         9698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_499097, whole genome shotgun sequence.
ACCESSION   DASUJG010000028 DASUJG010000000
VERSION     DASUJG010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9698
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499097"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..633)
                     /gene="ampD"
                     /locus_tag="VFX38_00525"
     CDS             complement(28..633)
                     /gene="ampD"
                     /locus_tag="VFX38_00525"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008737298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEX5313380.1"
                     /translation="MKAGATAIEDGWLVGARRMASPHGDERPSGTVISLVVIHGISLP
                     PGEFGGDFVESFFGGCLDVAAHPYFASIRELRVSPHLYVRRDGAMRQFVAFERRAWHA
                     GISCWRGRAACNDFSIGIELEGTDEIPYSDAQYTALERVLPVLGRAWPAIGAEGIVGH
                     ADIAPGRKSDPGVAFDWPRLAAGLARAGYNFRRSRHEACSP"
     gene            complement(630..1091)
                     /locus_tag="VFX38_00530"
     CDS             complement(630..1091)
                     /locus_tag="VFX38_00530"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX5313381.1"
                     /translation="MSDERELYCLLLPLGGGKLLLPRRIVEEVRGLVPPTPVPGAPAW
                     LLGCIPWRDGRIPLLAMEPLLDARVPDSSRRSRMVIVRAPATTLKPPAIAILTQGFPY
                     ILRVTPTLLGSSFVPRHEALLAELNLGFERPVIPDLPALAEEAVEFLAARG"
     gene            complement(1088..5173)
                     /locus_tag="VFX38_00535"
     CDS             complement(1088..5173)
                     /locus_tag="VFX38_00535"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5313382.1"
                     /translation="MTRAIAAGARGVETTDAFLALSSVLRPLLAAARNELDRDAAARD
                     RAGMAAAFLARIDPLARVLSVSGPPAAARLAARMTALAKHLEAAAAPGEEKRLALLVR
                     ACLRLEGHFVSPPPASDEPSADGDDRLGGLARTAALREARLEVLRMGARLDDRWHGIG
                     APASLPGFVATAGRLASVLAFLDIPGPAQCLQGLASLFGDRTEMVAKANRELQNRIAD
                     VLIAIAHDLASRQRNGGERGCANEYSDSALAAIAAVVETLEPATPSAVSVPPAATIDW
                     PPVRAERLETTVAEIFLRETSVIVPKLDAHCVHWRGNPVADRTALAALYRLFECLAGS
                     AATAGAPRLSAFSAAIAELLHRLQTGLLPNTPARVAIVSQFVAMMPRLCEEFASGTEA
                     TADAAALLREAREVGRLDASAAEDAAAGNDDVGAGTSPEPQADGSARIPRLEPELYAI
                     YRSEALEHLAVLDSAGSAWERGEEFAIGEEPRRAAHTLAGCSKMAGVAEMGTLMSELE
                     EVLERAAGAGTDPRALLVPMAIATDTARALVHAYGDGQSALPSVREAQERLKALRDVL
                     PPRPAVSPDDSGDATQDSAAAGGDPELVQLFTGEAAELLEGADASLRAWRADPSASEP
                     ASELLRRLHTLKGGARMAGLAPFADLVHELETFLTRIVDGMLEVSAGRLELAEHALDN
                     LHGMLEELADARQPTPADGLRAELRSESERAAAALAVRAVLPEASEADEGQEADQPRS
                     RDFRPGLARVRTDLLEAAINGAGEVGIYRGRVGQQLGAMNVHLGELERTVERLRSQMR
                     ELEMEVEAQIISTREQRRAANLAEFDPLEFDRYTHIHELSRSLAEAVSDLTSLRSLFA
                     TGLRLAGTALDRQGRVNAELQDALLRTRMMPFALSEGRLRRLVRLVAEESGKRIDFRF
                     TDMGGEMDRLVLDHVLPALEHLLRNAVVHGIESVAERRRAGKPAAGRIELLQRREGAE
                     TVVEVADDGGGFDFAAIRARAEREGLIEPAAAVSEGELVDLVFRLGFTTADRLSQTAG
                     RGVGMNVVAAEARYVGGTVEIVSRAGEGTRMLLRFPATLAIGHSLLIRVGTRRYLVPL
                     AGIGSIARVARTLLEGHLKSDTPSYEQGGESWQLVSLARALGEEAAPPENQADRVPLL
                     LIRVGNRRVAFVADDMEGSREVVIKPVGPQVAHIAGISGATFFDDGSIGLLLDLAGLV
                     RATGQWETGPAPAPSGAASRREPLVMVADDSITVRRVTERLLARHAVRVLGARDGVEA
                     LELLADHKPDLLLLDIEMPRMNGYELAEHMRNDERLREIPIIIITSRVGEKHRARAAA
                     LGIRHYLGKPYLESELLGAIRSVMGGLETPAAAGGDTAQAS"
     gene            complement(5170..7263)
                     /locus_tag="VFX38_00540"
     CDS             complement(5170..7263)
                     /locus_tag="VFX38_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX5313383.1"
                     /translation="MSRGYTARATFLTYVVGFAALICLVLAAADFFFVAQGAGYDTEY
                     FSELTAVAGESQALPAAARRAFGGDEKAFAQLAQRIKATSAALQLLANGDPATLMPAP
                     KGRVAVEVKLLQQRWSSTLPAAQTILSAKNSILYVQQTAARVRAAMPVYVASWQKLIQ
                     QLAEEGRSLSLMPTAAAQPVLAEQTREEMEALLAGRGDLPQVSTSFNQHVADFGTVLD
                     VFTHGSKTLKIAPLPHSSDLSDSLQALNQQYAVLSGELQPLAAASASIIAWHQALAAL
                     DTARPDMLTDITAINQAYQDQIAARPFKPLYGYIFGALALAFIILLVLRYQLTSEARR
                     AARAQQVQNERNQKGILRLLDELSTLADGDLTVELAVTDDITGAIADSINYTIGALRE
                     LVRTIRDTAVEVDGAARQAEGTTSHLAEASENQSRQITGASTSITQMARAIGQVSANA
                     EEASEVSRRSVEIAHKGGEAVRRTIEGMTSIREAIQDTAKRMKRLGESSQEIGDIVEL
                     IDEIAEQTNILSLNAAIQASTAGEAGRGFGVVADEVQRLAVRAASATRQIETLVRTIQ
                     SDTNEAIASMEQSTAGVVSRAELAENAGHALDEIESVSNDMAALIENISGVAREQAAV
                     AGDISGSMGVIQGITSQTAEGSAVTARSIGKLAALAAELRRSVAGFRVSTEISANLAL
                     ADAKALPAKAGDAGR"
     gene            complement(7322..7873)
                     /locus_tag="VFX38_00545"
     CDS             complement(7322..7873)
                     /locus_tag="VFX38_00545"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX5313384.1"
                     /translation="MAAARLAVRYDRPFELLLGLEEAILGPGRTEAGVRADSWAGIAF
                     RVARCAFVAPREEVREVLAWPGVSRLPRAQPWLLGLANVRGQLIAVSDLGRWSGIAAS
                     PPVNAGRVIVVNHPEIPAGLLIHRVEGFRRFTGEEAVAPPDGIPARMAPFVGGAFERG
                     GERWTVLGLRALVASESFLQAAC"
     gene            complement(7875..8237)
                     /locus_tag="VFX38_00550"
     CDS             complement(7875..8237)
                     /locus_tag="VFX38_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010132438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5313385.1"
                     /translation="MSKVLIVDDSPTDVHVMRTALEKGGFEVISAKDGETGLALAREE
                     RPDAVIMDVVMPGVNGYQATRQLTHDPETASIPVVFVTGKEQPTDRVWGMRQGAVAYL
                     TKPVTAEQLVAKIREITA"
     gene            complement(8250..8660)
                     /locus_tag="VFX38_00555"
     CDS             complement(8250..8660)
                     /locus_tag="VFX38_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5313386.1"
                     /translation="MSEAAGELAGITVLVADDSETIRYATQSLLERKGARVLTAVDGF
                     EALAAIADGRPDLVFADVMMPRIDGYQLCALVKANAVFRSIPVVMLSAREGAFEAARS
                     RMAGSEEHLGKPFTEDELAAVVARHVSPRSAAAH"
     gene            8759..>9698
                     /gene="gshB"
                     /locus_tag="VFX38_00560"
     CDS             8759..>9698
                     /gene="gshB"
                     /locus_tag="VFX38_00560"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016567014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEX5313387.1"
                     /translation="MVTQLPATDRHSPRLAVIMDPIEAIHPEKDTSLALLLEGQSRGY
                     ELVYLPPAAIAAESGRARGRGRLIRVRDSATHWFEAGAHWEGPLESCDLILMRKDPPF
                     DQEYVYLTYLLELAERAGARVVNAPRALRDCNEKASLLRFPQFAPPTIVARDLPSLRE
                     FREREGRIVLKPLDGMGGRGVFVLGAEDPNLAAVFEMLSRGGREHIMAQRYLPEISDG
                     DKRVLLVAGRPVPFMLARVPAEAGGRGNLAAGARGEPRPLGAREREIAEAVGPILARE
                     GLLFVGLDIIGGRLTEINVTSPTCVRELDHAFDLNIA"
BASE COUNT         1603 a         3336 c         3248 g         1511 t
ORIGIN      
        1 gcaaggccac gacgatgacg aagaaattca cgggctgcaa gcctcgtgcc gcgagcggcg
       61 gaaattgtac cccgcgcgtg cgagtcccgc cgcgaggcgc ggccagtcga aggcgactcc
      121 gggatcgctc ttgcgcccgg gcgcgatgtc cgcgtgcccg acgattccct ccgcaccgat
      181 ggcgggccac gcgcggccga gcaccggaag cacgcgttcg agcgccgtgt actgcgcatc
      241 gctgtaagga atttcgtcgg ttccctcgag ttcgatgccg atggagaaat cattgcaggc
      301 cgcgcgcccg cgccagcacg aaattccggc atgccaggcg cgccgctcga aggcgacaaa
      361 ctgccgcatt gccccgtcgc ggcgcacata gagatgcgga gacacgcgca gttcccggat
      421 cgacgcgaag tacggatgcg ccgcaacgtc gaggcagccg ccgaagaaac tttcgacgaa
      481 gtcgccgccg aattcgcccg gcggcaagct gatgccgtga atgacgacga gggaaatcac
      541 ggttcccgac ggacgctcgt cgccgtgggg actcgccatc cggcgggctc cgacgagcca
      601 accgtcctcg atcgcggtgg ctccggcctt cacccgcggg ccgccaggaa ctcgaccgct
      661 tcctcggcga gtgccggaag atcgggaatc accgggcgct cgaagccgag attcagctcc
      721 gccagcaagg cctcgtggcg cggaacgaag gaggagccca gcagcgtcgg cgtcacgcgc
      781 aggatgtagg ggaagccctg cgtcaggatg gcgatcgccg ggggcttgag cgtcgtcgcc
      841 ggtgcgcgca cgatcaccat gcgcgagcga cgcgaggagt ccggcacgcg cgcatcgagc
      901 agaggctcca tcgcgagcag cggaatgcgc ccgtcgcgcc acggaatgca gccgagcagc
      961 cacgcgggtg cgccgggcac cggcgtcggg ggaacgagtc cgcgcacttc ttcaacgatc
     1021 cggcggggca ggagaagttt gccgccgcct agcggcagga gcaggcaata gagctcgcgc
     1081 tcgtcgctca tgatgcctgt gccgtgtcac cgccggccgc ggcgggagtc tcgagcccgc
     1141 ccatgaccga gcgaatcgca ccgaggagtt cggattccag atagggcttg ccgagatagt
     1201 ggcggatgcc gagtgcggcc gcccgggcgc gatgcttctc gcccacgcgc gaggtaatga
     1261 tgatgatcgg aatctcgcgc agccgctcgt cgttgcgcat atgctcggcg agttcatagc
     1321 cgttcatgcg cggcatttcg atgtcgagca gcagcagatc gggtttgtgg tccgcgagaa
     1381 gttcgagcgc ctcgactccg tcgcgggcgc cgagcacgcg caccgcatga cgtgcgagca
     1441 ggcgctcggt gacccggcga acggtgatcg aatcgtccgc gaccatgacg agcggctccc
     1501 ggcgcgaagc ggctcccgag ggcgccggcg ccggacccgt ttcccattgc ccggtcgccc
     1561 gcacgagtcc ggcgagatcg agcagcaggc cgatcgagcc atcgtcgaag aaggtcgcac
     1621 cggaaatgcc ggcaatgtgc gccacctgcg ggccgaccgg cttgatgacg acttcgcggc
     1681 tgccttccat gtcgtcggcg acgaaggcga cgcgccgatt gccgacccgg atcaggagca
     1741 agggcacgcg gtccgcctga ttttccggcg gcgccgcctc ctccccgaga gcgcgtgcga
     1801 gcgacacgag ttgccaggac tctccgccct gctcgtagct cggggtgtcc gatttgaggt
     1861 ggccttcaag aagggtgcgc gcaacgcgcg cgatgctgcc gatgccggca agcggcacga
     1921 gatagcggcg ggtgccgacg cggatcagca ggctgtggcc gattgcgagc gtcgccggaa
     1981 agcgcagcag catgcgcgtg ccctcgcctg cgcgcgatac gatttccacc gttccgccga
     2041 catagcgcgc ctcggccgcc acgacgttca tcccgactcc gcgcccggcg gtctggctga
     2101 gcctgtcggc ggtcgtgaag ccgaggcgaa agaccagatc cacgagttcg ccttcggaaa
     2161 ccgccgccgc cggttcgatc agaccttcgc gttcggcgcg cgcgcggatc gccgcaaagt
     2221 cgaaaccgcc gccgtcgtcg gcgacctcga ccaccgtttc ggcaccctcc cgtcgctgca
     2281 gcagttcgat gcgccccgcc gcaggcttgc cggcgcggcg gcgttccgcg acgctctcga
     2341 tgccgtgcac gacggcgttg cgcagaagat gctcgagcgc gggcagcaca tgatcgagca
     2401 cgaggcgatc catctcgccg cccatgtcgg tgaagcggaa gtcgatgcgt ttgcccgact
     2461 cctcggcgac caggcgcacg aggcggcgca acctcccctc cgagagcgcg aagggcatca
     2521 tgcgtgtgcg cagcagcgca tcctgcaatt cggcgttgac ccgtccctgg cggtcgagcg
     2581 cggtgccggc aaggcgcagg ccggtcgcga acagcgagcg cagactggtg agatcggaga
     2641 cggcttcggc gagcgaacgc gaaagctcgt gaatgtgcgt atagcgatcg aattccagcg
     2701 gatcgaactc ggcgagattc gcggcgcgac gctgttcgcg cgtcgagatg atttgcgctt
     2761 cgacttccat ctcgagctcc cgcatctggc tgcgcagacg ctccaccgtg cgctcgagtt
     2821 ctccgagatg aacgttcatg gcgccgagct gctgcccgac tcgcccgcgg taaatgccca
     2881 cctcaccggc gccgttgatg gcagcctcca gaagatccgt acgcaccctt gcgaggccgg
     2941 ggcgaaagtc gcggctgcgc ggctgatcgg cctcctgccc ctcgtccgcc tcgctcgcct
     3001 ccggcaggac ggcccgcact gcgagcgccg ccgcggcccg ctcgctttcg ctccgaagct
     3061 ccgcgcgcaa gccgtcggcg ggcgtcggct ggcgggcatc ggcgagttcc tcaagcatcc
     3121 cgtgcagatt gtccagcgca tgctcggcga gttccagcct cccggccgag acctcgagca
     3181 tcccgtcgac gatgcgcgtg agaaaagttt cgagttcgtg aacgagatcg gcgaacggag
     3241 caagaccggc catgcgcgcg ccgcccttga gcgtatgcag ccgccgcagc agctcgctcg
     3301 cgggctcgct cgccgacgga tcggcccgcc aggcgcgcag cgaagcatcg gctccttcga
     3361 gcagttccgc cgcctcaccg gtgaaaagct gcacgagttc gggatcaccg cccgcggccg
     3421 ccgaatcttg cgtcgcatcg cctgaatcgt ccggcgagac cgcaggtcgc ggcggaagaa
     3481 cgtcgcgcaa ggccttgagg cgttcctgcg cctcgcgcac cgacggcaat gccgattgcc
     3541 cgtcgccata ggcgtgcacc agtgcacgcg cggtatccgt tgcaatggcc atgggcacga
     3601 gcagcgcgcg cggatcggtg cccgcacccg cggcgcgttc cagaacctcc tcgagctcgc
     3661 tcatcagcgt gcccatctcc gcgaccccgg ccatcttcga gcagccggcg agcgtgtggg
     3721 cggcgcggcg cggctcctcg ccgatcgcga attcctcgcc acgctcccag gcgctgcccg
     3781 cggagtccag gacggcgaga tgttcgagtg cctcgctgcg atagatcgca tagagttccg
     3841 gctccaatcg cgggatgcgc gcggagccgt cggcttgcgg ctcgggagaa gtgcccgcgc
     3901 caacgtcatc gttgccggct gccgcgtcct ccgccgcaga agcgtcgagc cttcccactt
     3961 cgcgagcttc gcgcagcaag gccgcggcgt ctgccgttgc ttccgtcccg ctcgcgaact
     4021 cctcgcacag tcgcggcatc attgccacga attggctcac gatcgcgacg cgcgcgggag
     4081 tattcgggag caagccggtt tgcaggcggt ggagaagttc cgcgatggcg gcggaaaagg
     4141 ccgaaaggcg cggagcgccg gccgtcgccg cgcttccggc aaggcattcg aagagccggt
     4201 agagcgccgc aagcgcggtt cgatcggcga ccgggttgcc gcgccagtga acacaatggg
     4261 catccagttt cggcacgatg acgctcgttt cgcgcagaaa gatttccgcg acggtcgtct
     4321 ccaggcgctc ggcgcgaacg ggcggccagt cgatcgtggc cgccggcggg acggaaactg
     4381 cgctcggcgt tgccggttcc agcgtttcca cgacggccgc gatggcggcg agcgccgaat
     4441 cgctgtactc gttcgcgcag ccgcgttcgc cgccgttacg ctgccgcgaa gcgagatcgt
     4501 gcgcaatcgc gatcaacaca tcggcaatac gattctggag ctcccggttc gccttcgcga
     4561 ccatctccgt ccggtcgcca aagagacttg ccagaccttg caaacactgt gccggccccg
     4621 ggatatccag aaaggcgaga actgacgcca gacgcccggc cgtggcgaca aatcccggga
     4681 gcgacgctgg cgcgccgatg ccgtgccaac gatcgtcaag acgcgccccc atccgcagca
     4741 cttccagacg tgcctcgcgc agggccgcgg tgcgggcgag accgcccaaa cgatcgtcac
     4801 catccgcaga cggctcatcg ctcgccggcg gcggagaaac aaagtgcccc tcaagacgca
     4861 ggcaggcacg aacgagaagc gcgagccgct tctcctcgcc cggcgccgcc gcggcttcga
     4921 ggtgtttcgc gagtgccgtc atccgtgccg cgaggcgtgc ggcggcgggc ggtcccgaga
     4981 ccgagagcac gcgcgcgagc ggatcgatgc gggcaagaaa cgccgccgcc atcccggccc
     5041 gatcgcgcgc cgctgcatcg cgatccagtt cgtttcgcgc cgccgcaagc agcggacgca
     5101 gcaccgacga aagggcaagg aacgcgtccg tggtttcgac gccccgagcg ccggcggcga
     5161 tggcgcgcgt caccggcccg cgtcgccggc cttcgccggc aaggcctttg cgtccgcgag
     5221 cgcgaggttt gcggaaatct ccgtcgagac ccggaatccg gcgaccgagc ggcgcagttc
     5281 cgccgccagc gcggcgagct tgccgatgga acgcgcggtg accgcactgc cctcggcggt
     5341 ctgcgaggta attccctgga tcacgcccat cgatccggaa atgtcgcccg cgacggcggc
     5401 ctgttcgcgg gcgacgccgg agatgttctc gatgagcgcc gccatgtcgt tcgataccga
     5461 ttcgatctcg tcgagcgcgt gaccggcgtt ctcggcgagt tcggcgcggc tcacgacgcc
     5521 cgcggtggat tgctccatcg acgcgatcgc ttcgttggta tcggactgga tcgtgcgtac
     5581 cagggtttcg atctgacggg tcgccgaggc cgcgcgcacc gcgaggcgct gtacctcgtc
     5641 ggcaaccact ccgaagccac gtcccgcctc cccggccgtc gaggcctgga tcgcggcatt
     5701 gagcgagaga atattggtct gctcggcgat ctcgtcgatg agttcgacga tgtcgccgat
     5761 ttcctgcgag gactcgccga gacgcttcat ccgtttcgcg gtgtcctgga tcgcctcgcg
     5821 gatgctggtc atgccctcga tcgtgcggcg cacggcctcc ccgcccttgt gcgcgatctc
     5881 gaccgaacgg cgcgaaacct cgcttgcctc ctccgcgttg gcggacacct ggccgatcgc
     5941 gcgcgccatc tgggtgatgg acgtgcttgc gcccgtgatc tggcggctct gattttccga
     6001 tgcttccgcc agatgcgagg tggtcccttc cgcctgccgc gccgcaccgt ccacttcgac
     6061 ggcggtgtca cgaattgtgc ggaccagttc gcgcaacgcc ccgatggtgt agttgatgga
     6121 gtcggcgatg gcgccggtga tgtcgtcggt aaccgcgagt tccaccgtga gatcgccgtc
     6181 ggcgagcgtg ctcaactcat cgagtaggcg caggatgccc ttctgattgc gctcgttctg
     6241 gacttgctgc gcacgcgccg cacgccgggc ctcgctcgtc aactgataac gcagcacgag
     6301 aagaatgatg aaggcgaggg cgagggcgcc gaagatgtag ccgtaaagcg gcttgaaggg
     6361 gcgcgccgca atctggtcct gataggcctg attgatggcg gtgatgtcgg tgagcatgtc
     6421 cggccttgcg gtgtcgagcg ccgcgagcgc ctgatgccag gcgatgatcg aggcactcgc
     6481 ggcggccaac ggctggagtt cgccggaaag caccgcatac tgctgattca aggcctgcag
     6541 cgagtcgctc agatcgctcg agtgcgggag cggcgcgatc ttgagagtct tcgaaccatg
     6601 agtaaacaca tcgagaaccg tgccgaaatc cgcgacgtgc tggttgaacg aagtactgac
     6661 ctggggtaga tcgccgcggc ccgcgagcag tgcctccatc tcctcgcggg tctgctcggc
     6721 gagcaccggc tgggcggcgg ccgtcggcat cagcgaaagg gagcgtccct cctccgccag
     6781 ttgctgaatg agcttctgcc aggaggcgac gtagacgggc atcgccgcgc gcaccctcgc
     6841 cgccgtctgc tgcacgtaga gaatcgaatt tttcgccgag agaatcgtct gcgcggcggg
     6901 caaggtgctc gaccagcgtt gctgcaagag tttgacttcg accgcaacgc ggcccttcgg
     6961 cgccggcatc agcgtggccg gatcgccgtt tgcgaggagc tgcaacgcgg cgctcgtggc
     7021 cttgattcgc tgcgcaagtt gcgcgaacgc cttttcgtcg ccgccgaagg cccggcgggc
     7081 ggcagccgga agggcctgcg actccccggc gacggcggta agttcggaga aatattcggt
     7141 gtcgtagccc gcgccctggg cgacaaagaa gaaatccgct gccgcaagca cgaggcagat
     7201 caacgccgca aagccgacga cgtaggtcag gaacgtggcg cgagccgtgt atccgcggga
     7261 catcttcatt cacctgtgca gtcgattgtg cgcaccgtga ctggcgagcg gcgggcgccc
     7321 ctcagcaggc cgcctgcaaa aagctttccg aggcgacgag cgcgcgcaga ccgagcaccg
     7381 tccagcgctc tcctccccgc tcgaaggccc cgccgacgaa gggcgccatg cgcgccggaa
     7441 ttccatcggg aggagccacg gcttcctcgc ccgtaaaacg gcggaatcct tccacgcgat
     7501 ggatcagaag accggccgga atctccggat gattgactac gatgacccgg cccgcattca
     7561 cgggcgggct tgcggcgatt ccggaccagc gcccgagatc gctcactgcg atcaactgtc
     7621 cgcgcacgtt ggcaagtccg agcagccagg gttgcgcccg cggcaggcgg ctcacgccgg
     7681 gccaggcgag cacttcccgc acttcttcgc gcggagcgac gaacgcgcag cgcgcgacgc
     7741 gaaaggcgat cccggcccag gaatccgcgc gcacgccggc ctcggtgcgg ccgggaccga
     7801 ggatggcttc ctcgagtccg agcagcaact cgaagggccg gtcgtaacgg acggcgagcc
     7861 gggctgcggc catttcaagc cgttatttcg cgaatctttg cgaccagttg ttccgcggtg
     7921 accggcttgg tcagataggc cacggcgccc tgacgcattc cccagacgcg atccgtcggc
     7981 tgctccttgc cggtcacgaa gacgaccgga atggaggcgg tctcgggatc gtgcgtgagc
     8041 tggcgcgtcg cctgatagcc gttcacaccc ggcatcacga catccatgat gaccgcgtcg
     8101 ggacgctcct cacgggcgag cgcgagtccc gtctcgccat ccttcgcgct gatcacctcg
     8161 aagccgcctt tctcgagcgc ggttcgcatc acgtgcacgt cggtggggga atcgtcgacg
     8221 atcagaacct tgctcatgct ccttgctcct caatgcgcgg ccgcggaacg cggcgataca
     8281 tgccgcgcca ctaccgccgc cagttcgtct tcggtaaagg gcttgccgag gtgttcctcg
     8341 gagcccgcca tgcggctgcg cgccgcttcg aacgcaccct cgcgcgccga aagcatcacc
     8401 accggaatcg agcggaacac ggcgttcgcc ttcaccagcg cgcagagctg gtagccgtcg
     8461 attcggggca tcatgacgtc ggcaaaaacg agatcgggac ggccatcggc gatcgccgcc
     8521 agcgcctcga atccgtcgac cgccgtgagc acgcgtgcgc ccttgcgttc cagcagactc
     8581 tgcgtcgcgt agcggatcgt ctcggaatca tccgcgacca ggaccgtgat gccggcaagc
     8641 tccccggccg cttcgctcat cgccgcgcca cgccgtcgcc gccgggctca cgcggcccca
     8701 ccgccggcgc caccagcggg attttcgcgc acaatagagg ccctgccgcc atgagcgcat
     8761 ggtaacgcaa ttgcccgcca cagaccgaca ttcgccgcgg ctcgcggtga tcatggaccc
     8821 gatcgaggcg atccatcccg aaaaggacac cagcctcgcg ctcctgctcg aggggcagag
     8881 ccgcggttac gaactcgtct atctccctcc cgcggcaatc gctgcggaat cgggtcgcgc
     8941 ccgcggacgc gggcggctga tccgggtgcg cgattccgcg acgcattggt tcgaggcggg
     9001 cgcgcactgg gaagggccgc tggaatcctg cgacctcatc ctcatgcgca aggatccgcc
     9061 cttcgatcag gaatacgtgt atctcaccta cctcctggaa cttgccgaac gtgcgggcgc
     9121 gcgggtggtc aacgctccgc gcgcacttcg cgactgcaac gaaaaggctt cgctgctgcg
     9181 cttcccgcaa ttcgcgccgc cgacgattgt tgcgagagac cttccgtcgc tgcgcgagtt
     9241 ccgcgaacgc gaggggcgca tcgtgctgaa accgctcgac ggcatgggcg ggcgcggcgt
     9301 cttcgtcctc ggcgccgagg atccgaatct cgccgccgtc tttgaaatgc tgagccgcgg
     9361 aggacgcgag cacatcatgg cgcaacgcta cctgccggaa atcagcgacg gggacaaacg
     9421 cgtcctcctc gtcgccgggc gacccgttcc cttcatgctc gcgcgcgtgc ccgccgaggc
     9481 cggcggtcgc ggcaacctcg ccgccggcgc ccgcggcgag ccgcgtccgc tgggagcgcg
     9541 cgaacgcgaa atcgcggaag cggtcggccc gatactcgcc cgcgagggac tgctgttcgt
     9601 cggactcgac atcatcggcg ggcgactcac cgagatcaac gtgacgagcc cgacctgcgt
     9661 acgcgaactg gatcacgcct tcgatctgaa tatcgccg
//
LOCUS       DASUJG010000029         3552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1110879, whole genome shotgun sequence.
ACCESSION   DASUJG010000029 DASUJG010000000
VERSION     DASUJG010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110879"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VFX38_00565"
     CDS             complement(<1..408)
                     /locus_tag="VFX38_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313388.1"
                     /translation="MHIIVIRPDRESRRFTLGRRHAGAAVFGTFTIALAVAIGAYLLG
                     HGQRGAAQSKDRAQVASLKVKLAHKTAALTALQTKNQGGINAIAARMARLDAQVNQVA
                     AMSAQVVKLAGLKNSSFDFNAPPGEGGAAPAVEL"
     gene            430..879
                     /locus_tag="VFX38_00570"
     CDS             430..879
                     /locus_tag="VFX38_00570"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HEX5313389.1"
                     /translation="MKPQFRPRRLSLEASLGAIYHWSRELATLRERLAAALPAPEAEH
                     LAGVSLQADRLLVFADSTAWCTRLRYRASILETAVEPLLGLRPQLEFRTLPPVLPAAR
                     RQRCELSPVAAESLRAAARGVSEPALARALERLADSQPATGPSAGPA"
     gene            complement(843..1763)
                     /gene="lpxC"
                     /locus_tag="VFX38_00575"
     CDS             complement(843..1763)
                     /gene="lpxC"
                     /locus_tag="VFX38_00575"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEX5313390.1"
                     /translation="MVKQRTLKNSIRATGVGLHTGQKVFMTLRPAAADTGIVFRRVDL
                     DSPVEIKAEAHSVGDTTLGTTLVKGKVRVATVEHLLSALAGLGVDNAYVDLSAPEVPI
                     MDGSAGPFVFLIQSAGIEEQGVPKRFIRIKKAVEVHQDDKWARLEPYEGFSVGVEIEF
                     DHPIFKQHTCRSLVNFSSTSFVKEVSRARTFGFLRDIEYLRRRNLTLGASLDNAIALD
                     EFHILNEDGLRSEDEFVKHKVLDAVGDLYLLGCTLIGRYLAYKPGHGLNNAILRALLA
                     DARAYEEVVFEEREGVPISYAGPALGPVAG"
     gene            complement(1893..3065)
                     /gene="ftsZ"
                     /locus_tag="VFX38_00580"
     CDS             complement(1893..3065)
                     /gene="ftsZ"
                     /locus_tag="VFX38_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015005183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX5313391.1"
                     /translation="MFELMDGVSQEAVIKVMGVGGGGGNAVRHMVASKIDGVDFICAN
                     TDAQALRSTNAKTVLQLGAGITRGLGAGADPEVGRQAAIEDQERIREVIGNCNLLFIT
                     AGMGGGTGTGGAPIVAQIAKEAGALVVAVVTKPFAWEGKQRNAIADHGIGELGRYVDS
                     LITIPNDKLLKVLDSKTSLLDAFGSANDVLAGAVQGIAELITRPGLINVDFADVRTVM
                     SEMGMAMMGSGAAQGEGRARKATESAIASPLLEDVSLSGVKGILANITAGMDLTIGEF
                     DEVGNILRDIASDDATVVVGTVIDPGMNDGELRVTVVATGLGRQRAEAERPEMKLVAQ
                     RGGAAPADAIGAGFDEKMIDIPTVIRKRREGREPGRQDPTDPRYLDIPAFLRRQAD"
     gene            complement(3109..>3552)
                     /locus_tag="VFX38_00585"
     CDS             complement(3109..>3552)
                     /locus_tag="VFX38_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007151175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HEX5313392.1"
                     /translation="QLASSEETIEVPSVGERPPRRLARQTLAEVVEPRYAELFALVQA
                     EVRRSGFEDMIAAGIVLTGGASKMEGAVELAEDIFHMPVRLGAPTGVAGLAEVVRNPI
                     HATGVGLLIYAREVLMGRRGAEHVPVARGFGDLFERMRRWFEGNF"
BASE COUNT          577 a         1218 c         1122 g          635 t
ORIGIN      
        1 aagttccacg gcgggcgcgg cgccgccttc tccgggaggc gcgttgaagt cgaagctgct
       61 gttcttgagt ccggcaagct tgacgacctg cgcgctcatc gccgcgacct gattgacctg
      121 cgcatcgagg cgcgccatgc gggcggcgat cgcgttgatg ccgccctggt ttttcgtctg
      181 cagcgccgtc agggccgccg tcttgtgcgc gagtttcacc ttgagactcg ccacctgcgc
      241 gcgatccttc gattgcgcgg cgccgcgctg accgtggccg agaaggtacg cgccgatagc
      301 caccgcgagc gcgatcgtga acgttccgaa gaccgccgct ccggcgtggc gccggcccag
      361 cgtgaagcgg cgcgactcgc gatccgggcg aattacaatg atgtgcacga actacgtcct
      421 cgggtcgcga tgaagccgca attccgcccg cggcggctca gcctcgaagc ctcgctcgga
      481 gcgatatacc actggtcgcg ggaactggca acgctgcgcg agcggctggc cgcggcgctg
      541 cccgcaccgg aagccgaaca ccttgccggc gtcagcctgc aggcggatcg tttgcttgtg
      601 ttcgctgatt ccaccgcttg gtgcactcgt ctgcgttacc gcgcgtcgat tctggaaacc
      661 gcggtcgagc cgcttctcgg cctgcgtccg caacttgaat ttcgtaccct gccgcccgtg
      721 cttcctgccg cccgccggca gcgctgcgaa ctgtcgccgg tggccgccga aagtctccgg
      781 gcagccgccc ggggcgtctc cgagccggcg cttgcccggg ctctcgagcg cctcgccgac
      841 tctcagcccg cgaccgggcc gagcgccggg ccggcgtagg aaatcggcac gccttcgcgt
      901 tcctcgaaaa ccacttcctc gtacgcgcgc gcgtccgcaa gcagagcccg caggatcgcg
      961 ttgttcagcc cgtgtcccgg cttgtaggca aggtaacgtc cgatcagggt gcacccgagg
     1021 agatacaaat ccccgaccgc atcgagcacc ttgtgtttca cgaactcgtc ttcggagcga
     1081 agtccgtcct cgttcaggat gtgaaactcg tcgagcgcga tcgcattgtc gagcgaggcg
     1141 ccgagcgtga ggttgcgccg gcgcaggtac tcgatgtcgc gcaaaaaccc gaaggtgcga
     1201 gcgcggctta cttccttgac aaatgaagtg ctcgagaagt tcacgaggct gcggcaggtg
     1261 tgctgcttga agatcggatg atcgaactcg atctcgacgc cgacgctgaa gccctcgtag
     1321 ggttcgagcc gcgcccattt gtcgtcctga tggacctcga ccgccttctt gatgcggatg
     1381 aagcgcttgg gaacgccctg ctcctcgatg cctgccgact gaatcagaaa cacgaacggc
     1441 cccgcacttc cgtccatgat cgggacttcc ggcgccgaga gatcgacgta ggcattgtcc
     1501 acgccgagac ccgcgagcgc ggagaggaga tgctccacgg tcgccacccg caccttgccc
     1561 ttgaccaggg tggtgccgag cgtcgtgtcg cccacgctgt gggcctcggc cttgatttcc
     1621 accggggaat cgaggtccac gcgacgaaac acgattccgg tgtccgccgc ggccggacgc
     1681 agggtcatga agaccttctg gccggtgtgc aggccgacgc cagtcgcccg gatactgttc
     1741 ttcagggtcc gctgtttaac cattgattcc gttgattccg cggtgcgcag acgcacgccg
     1801 ctgcccgggg agtcggggcg gcgcgacggg cgaagcttac cacaagtatc gtccgatgtc
     1861 attgagaaga gtccctccgc aagccctgcg gttcaatcgg cctggcggcg caggaaggcc
     1921 gggatgtcca gataacgcgg atccgtcgga tcctgccgcc cgggctcacg tccctcgcga
     1981 cgcttgcgga tgacggtcgg gatgtcgatc attttctcgt cgaaaccggc gccgatggca
     2041 tccgccggag cggccccgcc cctctgggcg acgagtttca tctccggccg ctcggcctcg
     2101 gcgcgctggc ggccgagccc ggtggcaacc acggtcaccc gcaattcgcc gtcgttcatg
     2161 cccggatcga tgaccgtgcc cacgaccacc gtggcatcat ccgaggcgat gtcgcgcaaa
     2221 atgtttccga cctcgtcgaa ctcgccgatg gtgaggtcca tcccggcggt gatattggcg
     2281 agaatcccct tgacccccga aagcgaaacg tcctcgagca gggggctcgc gatcgccgac
     2341 tcggtcgcct tgcgcgcccg tccttcgccc tgggccgcgc ccgaacccat catcgccatg
     2401 cccatctcgg acatgaccgt gcgcacgtcg gcgaagtcca cgttgatgag ccccgggcgg
     2461 gtaatcagtt cggcgattcc ctgcaccgcc cccgcgagca catcgttggc gctgccgaag
     2521 gcgtcgagaa gcgaggtctt ggaatccagc accttgagca gcttgtcgtt cgggatggtg
     2581 atcagggaat ccacgtagcg tccgagttcg ccgataccgt gatcggcgat tgcattgcgc
     2641 tgcttgcctt cccaggcgaa gggcttggtc acgaccgcga cgacgagcgc cccggcttcc
     2701 tttgcgatct gggccacgat cggagccccg ccggtgccgg ttccgccgcc catgccggcg
     2761 gtgatgaaga gcaaattgca attgccgatc acctcgcgga tgcgctcctg atcctcgatc
     2821 gcggcctggc gtccgacttc cgggtccgct cccgcgccga gtccccgggt gatgcccgcc
     2881 ccgagttgca gcacggtctt cgcattggtg ctgcgcaggg cctgggcatc ggtattggcg
     2941 cagatgaaat cgaccccgtc gatcttcgat gccaccatgt ggcgcacggc attgccgccg
     3001 ccgccaccga ctcccatcac tttgattaca gcctcctggc tgactccatc cattagttca
     3061 aacatgtcac acctcctgtg gtcggccgat tgaaacaaaa aacgagactc agaaattgcc
     3121 ctcgaaccag cggcgcatgc gctcgaagag atcgccgaag ccacgcgcaa cgggcacgtg
     3181 ctcggcgccg cgccttccca tgagcacttc gcgcgcgtaa atcagaagcc ccacgccggt
     3241 cgcgtgaatc ggattgcgca cgacttcggc gaggcccgcg accccggtcg gcgcgccgag
     3301 acgcaccggc atgtggaaga tgtcttcggc gagttcgacg gcgccttcca tcttggaagc
     3361 accgccggtg agcacgattc ccgccgcgat catgtcttcg aagccgctgc gacgaacctc
     3421 ggcctgaacg agcgcgaaga gttccgcata gcgcggctcg accacctccg cgagggtctg
     3481 gcgcgcgaga cggcgcggcg ggcgctcgcc gacgctcggc acctcgatcg tttcctcgct
     3541 cgaggcgagc tg
//
LOCUS       DASUJG010000030         2763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_133423, whole genome shotgun sequence.
ACCESSION   DASUJG010000030 DASUJG010000000
VERSION     DASUJG010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133423"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2029)
                     /locus_tag="VFX38_00590"
     CDS             complement(<1..2029)
                     /locus_tag="VFX38_00590"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HEX5313393.1"
                     /translation="MLGAGVMGAQIAAHLSAAGLAVLLFDLPGRDDPDGLVKDALRRL
                     GKLKPSPLASSATLEGITPCNYERDLARLAGCELVIEAVVERRDLKQSLYAKVAPHIG
                     ETALFATNTSGISIGELAGALPRELRPRFCGVHFFNPPRYMHLVELVAHAATDTAALD
                     RLEGFLTTALGKGVVRAKDTPNFIGNRIGVFAILAVLHHAERLKLPLDLVDRLTGPGI
                     GRPKSATLRTADVVGLDTLKHVVDNLAENLSGDPWHRYFRLPGWMEQLIAKGALGQKS
                     GAGVYRKGKQGIEVLDPVAGDYRTVKRALTEEMRAFLKERDPKKKYAMLAAIEHPQAE
                     FLRSVFLDLFHYTEHFLGEIAQCARDVDFAMRWGYGWKLGPFETWQAIGWREVAGDLE
                     KAIAAGETMSDVPLSAWAAEREAVHTPQGSWDAADGKLVAFSAHPVYRRQVFREHVVG
                     ETAPEFPTVFENESVRLWDLGDGVAVLSFRSKGHTIGPGVLAGVRESLALAERDWKAL
                     VIWQDSEPFSYGANLKEIGAGVAAGTLDPAKMIAEFQRTSLALRYSALPTVAAVRGMA
                     LGGGCEFLLHCDRVVAALESYIGLVELGVGLVPAGGGTKEFALRAAAETGPGWLFDRI
                     ARRFELIGTAKVAGSAEEARELGFLRESDVVVFHSDELLHVAKAQALALHES"
     gene            complement(2127..2582)
                     /gene="lptE"
                     /locus_tag="VFX38_00595"
     CDS             complement(2127..2582)
                     /gene="lptE"
                     /locus_tag="VFX38_00595"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEX5313394.1"
                     /translation="MPLPASLHATFVTGSQPYGNLENLLRRSLARSGVAVVAERGKAS
                     AVLTILRESSERRVRAVNADGRPAEYALDYRVRYQLLGDDGAVLLAPQSLHLSRDYAY
                     SPTVELGVGREQSAILAELQADAARLILLRLEALGRSIPAPAAASGGTR"
BASE COUNT          483 a          920 c          936 g          424 t
ORIGIN      
        1 ccgactcgtg gagcgcaagc gcctgcgcct tggcgacatg aagcagctcg tcggaatgga
       61 agaccacgac atcgctttcg cgcagaaatc cgagttcgcg cgcttcctcg gcgcttcccg
      121 caaccttcgc cgtgccgatg agttcgaagc ggcgcgcgat acgatcgaag agccagccgg
      181 gtccggtttc cgcggccgcg cgcagcgcga actccttggt gccgccgccg gcggggacca
      241 gaccaacgcc gagttcgacg agaccgatat agctttcgag cgccgcgacc acgcgatcgc
      301 aatgcagcag aaattcgcag ccgccgccga gcgccatgcc gcgcaccgcg gcgaccgtgg
      361 gcagcgccga gtagcgcagc gcgagcgagg tccgctgaaa ctcggcgatc attttggcgg
      421 gatcgagcgt gccggcggcg acgcccgcgc cgatttcctt cagattcgcg ccgtaggaaa
      481 agggttcgga atcctgccag atgacgagcg ccttccagtc acgctcggcg agcgcgagcg
      541 actcccgtac tcccgcgagc accccggggc cgatcgtgtg ccccttgctc cgaaacgaaa
      601 ggacggcaac gccgtcgcca agatcccaca gacgcacact ctcgttctcg aacacggtcg
      661 gaaattcggg cgccgtctcg ccgacgacat gctcgcgaaa cacctggcgg cgatagaccg
      721 gatgcgcgga gaatgcgacg agcttcccgt ccgcagcgtc ccaggagcct tgcggagtgt
      781 gcacggcctc gcgctccgcc gcccaggccg aaagcgggac atcgctcatc gtctcgccgg
      841 cggcgatggc cttttcgaga tcgccggcga cttcgcgcca gccgattgcc tgccaggtct
      901 cgaagggacc gagcttccaa ccgtagcccc agcgcatcgc gaagtccacg tcgcgcgcgc
      961 actgcgcgat ttcgccgagg aagtgctcgg tgtagtggaa aagatcgagg aaaaccgagc
     1021 gcaggaactc ggcctgcgga tgttcgattg ccgcgagcat cgcatacttc ttcttcggat
     1081 cgcgttcctt cagaaacgcg cgcatctcct cggtcaatgc acgtttcacc gtccggtaat
     1141 cgccggcgac gggatcgaga acctcgattc cctgtttgcc cttgcgatac acgccggcgc
     1201 ccgacttctg ccccagcgcg cccttcgcga tcaactgttc catccatccc gggaggcgga
     1261 aataccggtg ccagggatcg ccggaaagat tttcggcgag attgtccacg acatgtttca
     1321 gggtgtcgag gccgacgacg tcggccgtgc gcagggtcgc gctcttcggc cggccgattc
     1381 ccggaccggt gaggcgatcc accagatcga gcggcagctt caggcgttcg gcgtggtgca
     1441 acaccgcgag gatggcgaag acgccgatgc gattgccgat gaagttcggc gtatccttcg
     1501 cgcgcaccac acccttgccg agcgcggtgg tgagaaagcc ctcgaggcga tcgagcgcgg
     1561 cggtgtcggt tgccgcatgc gcgacgagtt ccaccagatg catgtagcgc gggggattga
     1621 aaaagtgcac gccgcagaag cgcggacgca attcccgcgg cagggcgccg gcgagttcgc
     1681 cgatggaaat gcccgaggtg ttggtcgcga agagcgcggt ctcgccgatg tgcggcgcaa
     1741 ccttcgcata cagagattgc ttgaggtcgc ggcgctcgac cacggcctcg atcacgagtt
     1801 cgcagccggc gaggcgcgcg agatcgcgtt cgtagttgca gggggtgatg ccttcgagcg
     1861 tcgccgacga ggcgagcggg ctcggtttca acttgccgag gcggcgcagc gcgtccttga
     1921 cgagtccgtc gggatcgtcc cgacccggca gatcgaagag cagcaccgcg aggcccgccg
     1981 ccgacaggtg ggcggcgatc tgcgcgccca tcacgccggc gcccagcact gccgcgcggc
     2041 caatattgcg aggggactcc tgcatcgtcc ggtcctgtgc gaaggcgagg cgcgcggagg
     2101 aatccgcgcc cgcttatttt agcgggtcag cgggttccgc cgctggccgc ggccggcgca
     2161 ggaatcgaac gcccgagggc ctcgagccgg agcaggatga ggcgtgccgc atcggcttgc
     2221 agttcggcga ggatcgcgct ctgctcgcgc ccgaccccga gttcgacggt cggcgaatag
     2281 gcatagtcgc ggctgagatg gaggctctgc ggagcgagga gcaccgcgcc atcatcgccg
     2341 agcagctggt agcgaacgcg gtagtcgagc gcatattccg ccggacgtcc gtcggcgttc
     2401 acggcccgga ctcgccgctc gctgctctcc cgcaggatgg tgagcaccgc gctcgccttg
     2461 ccgcgctccg cgactaccgc aacaccgctc cgcgcaagcg agcggcggag aagattctcg
     2521 aggttgccgt agggctgact gccggtgacg aaggtagcgt gcagcgacgc ggggagcggc
     2581 accgttccgg cgggctggaa tccgcaggcc gccaaagcgc atgcgacggt cagggcgaga
     2641 gcgaggcgaa gaacgccgag ctgcgcaggc ctcgcagctt cagccgagga cgacattgac
     2701 cagcctttcc ggcacgacga tgacccggca caccgcgcga tcgcccacgt gtcgccgcac
     2761 gtt
//
LOCUS       DASUJG010000031         2138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1233663, whole genome shotgun sequence.
ACCESSION   DASUJG010000031 DASUJG010000000
VERSION     DASUJG010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233663"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VFX38_00600"
     CDS             <1..264
                     /locus_tag="VFX38_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEX5313395.1"
                     /translation="IVRAVRDMGLEIRAGLHTGEVELEGDSVTGIGVHIGARVAALAH
                     GGETLVSRTVKDLVSGSGLRFEDRGSHMLKGVPEAWHLFAATS"
     gene            complement(387..1697)
                     /gene="aceA"
                     /locus_tag="VFX38_00605"
     CDS             complement(387..1697)
                     /gene="aceA"
                     /locus_tag="VFX38_00605"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEX5313396.1"
                     /translation="MNYDLESARLKKDWAESPRWKGIERRYTAEDVVRLRGSLHIEHT
                     LARHGAEKLWKLLHDEPYVSTLGALTGNQAMQQVKAGLKAIYLSGWQVAADANIAGEM
                     YPDQSLYPANSVPAVVRRINNTFIRADQIAHAEGKDDVDYFAPIVADAEAGFGGVLNA
                     FELMKSMIESGAAGVHFEDQLASVKKCGHMGGKVLVPTREAVEKLAAARLAADCLGVP
                     TILLARTDAEAADLLTSDVDDNDKPFCTGERTSEGFYRTRKGLDQAVSRGLAYAAVAD
                     LIWCETGKPDLEFAKAFAEAIRAKYPDKMLAYNCSPSFNWKKNLDDATIGRFQRELGA
                     MGYKFQFITLAGFHSLNYSMFNLAHGYARKGMSAFVELQEAEFAAAERGFTAVKHQRE
                     VGTGYFDAVTTTVQGAQSSTTALKHSTEDEQFLDAAKKLQVANG"
BASE COUNT          356 a          681 c          673 g          428 t
ORIGIN      
        1 atcgttcgcg ccgtgcggga tatgggtctc gaaatccggg caggtctgca caccggcgaa
       61 gtcgagctcg aaggcgactc ggtgacgggg atcggtgtgc acatcggcgc gcgtgtcgca
      121 gcgctcgcgc acggcggcga aacgctggtg tcccgcaccg tcaaggacct tgtctccggc
      181 tcagggctcc gcttcgagga ccgcggcagc cacatgctga agggagtccc cgaggcgtgg
      241 cacctgttcg ccgcaacgag ttgaaccggt ccggggtagc gagctgaacc ggtccggggt
      301 cgcggcggga aaccggccgg ggcatcgggg ccgtaggtgg ttgcgcgcgc cgggtatcca
      361 tcccggcgcg cttgtgccgc cgcttctcag ccgttcgcga cctgcagttt cttcgccgcg
      421 tcgaggaact gctcgtcctc ggtcgagtgc ttgagcgccg tggtcgacga ctgcgcgccc
      481 tgcaccgtcg tcgtcaccgc atcgaagtac ccggtaccga cctcgcgctg gtgcttgacc
      541 gccgtgaagc ctcgctcggc cgcggcaaac tcggcctcct gaagttcgac gaaagcgctc
      601 atgcccttgc gcgcgtagcc gtgcgcgagg ttgaacatcg agtagttgag gctgtggaaa
      661 cccgccagcg tgatgaactg gaacttgtag cccatcgcac ccagctctcg ctggaacctt
      721 ccgatcgtcg cgtcgtccag gttcttcttc cagttgaacg aaggtgagca gttgtaggcg
      781 agcatcttgt ccggatactt tgcgcggatc gcctcggcaa acgccttcgc gaactcgagg
      841 tccggcttgc cggtctcgca ccagatgaga tccgcaacgg cggcataggc gagcccgcgc
      901 gacacagcct ggtcgagccc cttgcgggtg cgatagaagc cttcactcgt tcgttcgccg
      961 gtgcagaacg gtttgtcgtt gtcatcgacg tcggaagtca gcagatcggc cgcttcggcg
     1021 tcggtgcgtg cgagaaggat tgtcggaacc ccgaggcagt cggcggcgag acgggccgcg
     1081 gcaagcttct cgaccgcttc gcgcgtcggc accagcacct tgccgcccat gtggccgcac
     1141 ttcttgaccg acgcgagctg atcctcgaag tggactcctg cggcgccgga ctcgatcatc
     1201 gacttcatca actcgaacgc gttcagcacg ccaccgaatc ccgcttcggc atcggcgacg
     1261 atcggtgcga agtaatcgac gtcgtccttg ccttcggcat gcgcgatctg gtcggcgcgg
     1321 atgaacgtgt tgttgatcct gcgcactacc gctggaaccg agttcgccgg gtagagcgac
     1381 tggtctggat acatctcgcc ggcgatgttc gcgtcggcag cgacctgcca gccggagaga
     1441 tagatcgcct tcaggccggc tttcacctgc tgcatcgcct ggttgccggt cagtgcaccc
     1501 agcgtgctca cgtagggttc gtcgtggagg agtttccaca acttctcggc accgtgccga
     1561 gcgagcgtgt gctcgatatg cagtgagccg cgcaggcgga cgacatcctc ggccgtgtac
     1621 ctgcgttcga tgcccttcca gcgcggactc tcggcccagt ccttcttcag ccttgccgat
     1681 tcgagatcgt aattcatgta ttgctcccat cgatgccgtg ccggggacga aaccccggcg
     1741 gattggtcag cggcgcccgc tcgctagcgg acgccctcga gcgcgccacg ccggacaacg
     1801 ccggtgcgac cgctgcacgg accggccagg gatcgcctgg ctgatctgcg ggcatggatg
     1861 ggtacccctt gcccttcgca cgctttgcct cgtgtgatcc gccgcggcac gccgtgcatg
     1921 acgtcaaatt atcagagtcg cccgtgcatc gcaagcagat tggcgcactg caataaaatt
     1981 tttgtcgcta taaatcaaca ctttagaatt agtttccatt ctatggaaat taacaccgct
     2041 tgttcggtac aaacgcgtgc tgcgatgcgt aagcttgtgc gctctcgacc cgattggaat
     2101 ttctaaaccg cgtcaactcg ttgaatcgaa acggttcg
//
LOCUS       DASUJG010000032         3775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1724343, whole genome shotgun sequence.
ACCESSION   DASUJG010000032 DASUJG010000000
VERSION     DASUJG010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1724343"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..1316)
                     /locus_tag="VFX38_00610"
     CDS             complement(33..1316)
                     /locus_tag="VFX38_00610"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX5313397.1"
                     /translation="MRTLITTALIGGVLALAVTTALAQTAASAPPTATNTGHRASNPA
                     HSSGGTNGTPPASGANAVREVPVSATMPAPAAASTVEVQLVSAASKFYPREALLRGIG
                     GCVVLSMFISASGHPIVMSAAFAEPPGVFDAAALEAASGFRYQVTDVAGGPVDAETLP
                     RGVNIQEPILYFIEGDGDWADWLCGLPLPHTLIVSPATAGAPDAIGIVSRDRQSPSTM
                     LSDMIGSERAILLQPQLMAHDLVTLPVPAGQTLTSGWVKVSFCIGTDGRVANPRVTDA
                     SPRGLYDQAAIAALSTWQFSPRKRNGRNVRVCKLTYTIPVIARRDLERGPVAVQAAST
                     VVNLHSGSDDSRRQDYIPGGKVVTRFCVEANGSLSHIEALKPQLPNPLAVAAVDVLES
                     FQYWPRQIDGKWVRTCNIEQTLQFSGGRSLLSVHG"
     gene            1337..2386
                     /locus_tag="VFX38_00615"
     CDS             1337..2386
                     /locus_tag="VFX38_00615"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX5313398.1"
                     /translation="MAGMQAKEADVTVWAPGLAVETTMTQFENLVFKGGGAKCIAYAG
                     AIAALEARGTLADVRRIAGTSTGSAAAVLLALGADGAALARIFSATSFSAFEDGSFGF
                     FPNVWRLFTRYGWFKGDAFSQWLRREIAALCGDENLSFAALAERAYDEPQRFRELYVV
                     GTDLSAQRAGVFSFETTPDVPLWLAVRISMSMPLLFAAVDYAGDVWVDGGLSWNYPIE
                     IFDRARYVNGAAGDGNVRVYNPATLGFRVDTRAQITAEREGRYLPAIKVGNFLAYAKA
                     FVNFAIGMANKSHLEPADWHRTVFIDVREVGTTDFSIGAAGIAALIESGRRDTESYFR
                     WFETATGEDAPLNRI"
     gene            complement(2536..>3775)
                     /gene="gatB"
                     /locus_tag="VFX38_00620"
     CDS             complement(2536..>3775)
                     /gene="gatB"
                     /locus_tag="VFX38_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006787154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX5313399.1"
                     /translation="AEIAERSVFARKNYFYPDLPKGYQISQYELPVVKGGRLVIALAD
                     GSRKTIGITRAHLEEDAGKSLHENFADATGIDLNRAGVPLLEIVSEPELRSAAEAVAY
                     MKAIHSLVRYLGICDGNMQEGSLRCDANVSIHRPGKAFGTRAEIKNLNSFRFVEQAIE
                     YEIARQAEILEAGGAVVQETRLYDPDRHETRPMRAKEEANDYRYFPDPDLLPLVIERS
                     FIEGVRDQLPELPAAKRARFESEYGLSAYDAGVLTSERDLADYYEALVAAIGGEPKLA
                     ANWVMGELAAALNREGLAIASSKLGAPVLAGLIGRVVDGTVSGKLAKEIFEAMWAGEG
                     EADVVIEKRGLKQISDPAALAKIVDDVIAANPAQLRQYRDGRDKLFGYFVGQAMKATQ
                     GKANPAELNRLLKEKLSTNA"
BASE COUNT          651 a         1171 c         1239 g          714 t
ORIGIN      
        1 aagaccgcat ggcaccacgc tcatcgatgt ccctaaccgt gaacgctgag caggctgcgg
       61 ccgccgctga actgcagggt ctgctcgatg ttgcaagtcc gcacccattt gccgtcaatt
      121 tggcgaggcc agtactgaaa cgactcgagc acgtccacgg cggcgacggc taacggattc
      181 ggcaattgcg gtttgagcgc ctcgatgtgg ctgagcgagc cgttggcttc cacgcaaaac
      241 cgcgttacca ccttgccgcc cggaatgtaa tcctggcggc ggctgtcgtc cgatccggag
      301 tgcaggttga cgaccgtcga cgccgcctgc accgcgaccg gtccgcgttc cagatcacgg
      361 cgcgcaataa cgggaatcgt ataggtcagt ttgcagaccc ggacgtttct gccgttgcgc
      421 ttgcgcggcg aaaattgcca ggtcgagagc gcggcaatcg ccgcctgatc gtacagtccg
      481 cgtggggaag catccgtcac ccgcggatta gcaacgcgtc cgtcggtgcc gatgcaaaag
      541 ctaactttca cccagccgct cgtcagcgtc tggcccgccg gcacgggcag ggtcaccaga
      601 tcgtgagcca tgagttgcgg ctgcaggaga atcgcccttt ccgagccgat catgtccgag
      661 agcatcgttg aggggctttg cctgtcacgc gacacaatgc caatggcgtc gggcgcgcca
      721 gcagtagcgg ggctcacaat cagagtgtgg gggagcggca atccgcataa ccaatccgcc
      781 cagtcgccgt caccctcgat gaaataaagg atcggttcct ggatattgac gccgcgcggg
      841 agagtctcgg catcaaccgg cccgccagcc acgtcggtaa cctggtaacg aaaaccgctg
      901 gccgcttcga gggccgcggc atcgaagacg ccgggtggct cggcaaacgc ggcgctcatc
      961 acgatcggat gcccgctcgc acttatgaac atgctcaata cgacgcagcc gccgatacct
     1021 cggagcaagg cctcacgggg ataaaacttg ctcgcggccg agaccagctg cacctcgacc
     1081 gtgctcgccg ccgccggcgc cggcatggtg gcggaaacgg ggacctcgcg gacggcgttt
     1141 gcaccggacg cggggggggt tccgtttgtg ccgccggaag aatgcgccgg gttgcttgcc
     1201 ctgtgccccg tattcgtcgc ggtcggcggg gcgctcgccg ccgtctgtgc gagggcagtc
     1261 gttacggcca gcgcgagaac tcccccaatc agcgccgtag ttatcagagt gcgcatgggc
     1321 tttttatagc agaggcatgg cggggatgca agcgaaggaa gccgatgtta cagtttgggc
     1381 gcccggcctc gcggtggaga cgacgatgac gcagttcgag aatctggtct tcaagggcgg
     1441 cggagccaaa tgcattgcct acgcaggcgc gatcgccgcg ctggaagcgc gcgggacgct
     1501 cgccgatgtg cggcgcatcg ccggcacgtc gacgggttcg gcggcggcgg ttctgctcgc
     1561 gctcggtgcg gacggagccg cactcgcacg gatcttttcg gccacctcct tcagcgcctt
     1621 cgaagatggg agcttcggct tcttccccaa cgtctggcgc ctgtttacgc gctacggctg
     1681 gttcaagggc gacgccttct cacaatggct ccggcgcgaa atcgccgcgc tctgcggcga
     1741 cgagaacctg agcttcgcgg cgctcgcgga gcgtgcttac gacgagccgc agcgttttcg
     1801 cgagttgtac gtcgtcggta ccgatctctc cgcacaacgg gcgggggtgt tctcgttcga
     1861 aacgacgccc gacgtcccgt tgtggcttgc ggtgcgcatt tcgatgagca tgccgctgct
     1921 gttcgcggcg gttgactacg cgggcgacgt gtgggtggac ggcggactga gctggaacta
     1981 cccgatcgag attttcgacc gcgcgcgtta cgtcaatggt gccgccggcg acggcaatgt
     2041 ccgcgtgtac aacccggcga cgctcggctt tcgtgtggat acccgcgcgc agattaccgc
     2101 cgagcgcgaa ggccgctatc tgcctgcgat caaggtcgga aatttccttg cctacgcgaa
     2161 ggcgttcgtg aacttcgcga tcggcatggc caacaagtcg cacttggaac cagcggactg
     2221 gcatcgcacg gtgttcatcg acgtgcgcga ggtcggcacc acggattttt cgatcggcgc
     2281 ggccgggatc gcggccctga tcgagagcgg gcggcgcgac accgagtcct actttcgatg
     2341 gttcgaaacc gcgacgggcg aggacgctcc gctcaaccgc atttgatcga cgttgcgttc
     2401 cccctgcgct cgcgcaatga cgcatctctt tcccccttcg aaaggaaggg ggatgcagag
     2461 ggatgtcttc ggggcggaac atttcccgcg caagagcgcg gcccacctcg ttcgaagggg
     2521 gcaaagacgg gcgggtcagg cgttggtcga gagtttttct ttgagcagac ggttgagttc
     2581 cgcaggattg gccttgccct gcgtcgcctt catggcctgg ccgacgaagt agccgaaaag
     2641 cttgtccctg ccgtcgcggt attgccggag ttgtgccgga ttcgccgcaa tcacgtcgtc
     2701 cacgattttg gcgagtgccg cgggatcgct gatctgtttc aatccgcgct tttcgatcac
     2761 gacgtcggcc tcgccttcgc cggcccacat cgcctcgaag atttccttcg cgagtttgcc
     2821 cgagaccgtg ccgtccacga cccgcccgat cagcccggcg agcaccgggg cgccgagttt
     2881 cgacgaggcg atcgcaagcc cttcgcgatt gagcgcggcg gcgagttcgc ccatcaccca
     2941 gttggcggca agcttgggct ccccgccgat tgcggcgacg agcgcttcgt aataatcggc
     3001 gaggtcgcgt tcggaggtga gcacgccggc atcgtaggcg gacagaccgt actcactttc
     3061 gaagcgcgcg cgtttcgcgg cgggtaactc ggggagctgg tcgcgcacgc cttcgatgaa
     3121 ggaacgctcg atcaccagcg gcaggagatc gggatcgggg aagtagcggt aatcgttcgc
     3181 ctcttccttg gcacgcatgg ggcgcgtttc gtgacgatcg ggatcgtaga gccgcgtttc
     3241 ctgcacgacg gcgccgccgg cttcgaggat ttccgcctga cgtgcgattt cgtattcgat
     3301 cgcctgctcg acgaagcgga aagaattgag attcttgatt tcggcgcgcg tgccgaaggc
     3361 cttgcccggg cggtgaatcg aaacattcgc atcgcagcgc agcgatccct cctgcatgtt
     3421 gccatcgcaa atgccgaggt aacgcaccag tgaatggatc gccttcatgt aggcgacggc
     3481 ctcggcggcc gagcgcagct ccggttccga gacgatttcg agcagtggca cgccagcgcg
     3541 gttgagatcg atcccggtcg cgtcggcaaa gttttcgtgg agcgattttc cggcgtcctc
     3601 ttcgagatgc gcacgggtga tgccgatggt tttccggctg ccgtcggcga gcgcaatcac
     3661 gaggcgcccg cccttcacca cggggagttc gtactggctg atctgatagc ccttgggaag
     3721 atcgggatag aaataattct tgcgcgcgaa caccgaacgc tccgcgatct cggcc
//
LOCUS       DASUJG010000033         3935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1724424, whole genome shotgun sequence.
ACCESSION   DASUJG010000033 DASUJG010000000
VERSION     DASUJG010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1724424"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..123)
                     /locus_tag="VFX38_00625"
     CDS             complement(<1..123)
                     /locus_tag="VFX38_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX5313400.1"
                     /translation="MSKKGENPPALPVLPLRDVVVFPHMVQNLFVGRVTSIKALD"
     gene            complement(200..1483)
                     /gene="clpX"
                     /locus_tag="VFX38_00630"
     CDS             complement(200..1483)
                     /gene="clpX"
                     /locus_tag="VFX38_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012981987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX5313401.1"
                     /translation="MNEEPRGPRDGGKVLFCTFCNKSQHEVGKLIAGPSVYICDECVE
                     LCNEIIREEMQGRNESAASKLPRPKEIKAILDQYVIGQKDAKKVLSVAVYNHYKRVEV
                     LGGRDEVELAKSNILLIGPTGSGKTLLAETLARILNVPFTIADATTLTEAGYVGEDVE
                     NIIQKLLQKCDYDVEKAQTGIVYIDEIDKLSRKSDNPSITRDVSGEGVQQALLKLIEG
                     TTASVPPQGGRKHPQQEFLQVDTRNILFIVGGAFAGLEKIIRNRTDKSGIGFVAEVKD
                     RIALQAEESIVLRQVEPEDLIRYGLIPEFVGRLPVIATLEALDEAALVRILVEPKNAL
                     IKQYKRLFDMENVELEFREEAIAECARRALARKTGARGLRTILERALLDSMYELPALE
                     GVARIVVDDKAIRGETRPYVVFDQSERSRAAASDE"
     gene            complement(1608..2234)
                     /gene="clpP"
                     /locus_tag="VFX38_00635"
     CDS             complement(1608..2234)
                     /gene="clpP"
                     /locus_tag="VFX38_00635"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEX5313402.1"
                     /translation="MSEISGQALVPIVVEQTPRGERAYDIYSRLLKERVVFVVGAIED
                     HIANLVVAQLLFLESENPDKDISLYINSPGGSVTAGLSIYDTMQFVKPDVSTICLGQA
                     ASMAAVLLAGGAKGKRYCLPHSRMMIHQPLGGVQGQQSDIEIHAREILTIRGRLNQIL
                     AEHTGQPVEKVQTDTDRDRFLSADEALEYGLLDSILKNRGAAAKERSP"
     gene            complement(2231..3559)
                     /gene="tig"
                     /locus_tag="VFX38_00640"
     CDS             complement(2231..3559)
                     /gene="tig"
                     /locus_tag="VFX38_00640"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX5313403.1"
                     /translation="MQVSVEEQGALKRRLSVTIPGESIEAEIVRRLQGIGKRAKIKGF
                     RPGKAPAKIVRQQYESAVREDVLEELRRSHYSDAVSEKSLIPVGNPRFAGGETAPDGS
                     YTFTADLEVYPQLDPQGLDDLKLLRPKVEIGDADVEEVLGRLRRQRGRWIPAERPARS
                     GDRLILDFDGRVDGEAFEGNSAKELALVLGSGDTLPGFEAALEGIGAGESRGFDLAFP
                     RGYRRTELAGRPAHFDVLVHRVEELEVPALDDAFAEEFGVAEGGVAELRSRIRTNMAG
                     EAEERVRAELLRQVSDQLVAANPVDVPTALLEDEIARQKRAALRRLGITPEPRQVSSI
                     PREPFEATAQRRVQTGLILSSLIEREAFRPDPALVERRIAELTRDAEDPGAEARKIRA
                     DNDAMRQVEALVLEDMAYNWLLGRAAVSDEPRSFFEFMEPPEGGRDRSKE"
     gene            complement(3594..3680)
                     /locus_tag="VFX38_00645"
     tRNA            complement(3594..3680)
                     /locus_tag="VFX38_00645"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3645..3647),aa:Leu,seq:tag)
     gene            complement(3755..>3935)
                     /locus_tag="VFX38_00650"
     CDS             complement(3755..>3935)
                     /locus_tag="VFX38_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313404.1"
                     /translation="EYLRLRGVGVIHGGAHSLRYTPHFAIGDEEVELVLRATREALLE
                     GPGSRQKAAAESEAA"
BASE COUNT          623 a         1324 c         1237 g          751 t
ORIGIN      
        1 gtcgagggcc ttgatggaag tcacccggcc gacgaagaga ttctgcacca tgtgcggaaa
       61 aacgacgacg tcgcgcagcg gcaacaccgg cagggccggc ggattttcgc ccttcttgct
      121 catcggggaa cctgcggaaa ctcgcccatt aggtggcggc gcgcggcggc gatttcaagg
      181 cgccgcggct gcgctgcgcc tattcgtcgg atgcggcggc gcggctgcgc tcggactgat
      241 cgaagaccac atacgggcgg gtctcgccgc gaatcgcctt gtcgtcgacg acaatccgcg
      301 cgacgccctc gagcgcgggc aattcgtaca tgctgtcgag gagcgcgcgt tcgagaatgg
      361 tgcggagacc ccgggcgccg gtcttgcgcg cgagcgcgcg ccgcgcgcat tcggcaatcg
      421 cttcctcgcg gaactcgagt tcgacgtttt ccatgtcgaa gaggcgcttg tactgtttga
      481 tcaacgcgtt cttcggttcc accaggatgc gcaccagcgc cgcctcgtcg agcgcctcca
      541 aggtcgcgat caccggcaac cgcccgacga attccggaat caggccgtag cggatgagat
      601 cctcgggctc gacctgccgc agcacgatgc tctcctcggc ctgcagggcg attcgatcct
      661 tgacctcggc cacgaagcca atgccgctct tgtcggtgcg gttgcggatg atcttctcga
      721 ggccggcaaa ggcgccgccg acgatgaaca gaatgttgcg cgtgtccacc tgcaggaatt
      781 cctgctgcgg atgcttgcgc cctccctgcg gcggcaccga ggcggtcgtg ccctcgatca
      841 gtttgaggag cgcctgctgc acgccctcgc cggagacgtc gcgcgtgatc gagggattgt
      901 cggatttgcg cgaaagcttg tcgatctcgt cgatgtaaac gatgccggtc tgcgcctttt
      961 cgacatcgta gtcgcatttc tgcagcagct tctggatgat gttctcgaca tcctcgccga
     1021 cgtacccggc ttcggtgagc gtcgtcgcat cggcgatggt gaagggcacg ttgagaatgc
     1081 gcgccagggt ttcggcgagg agcgtcttgc cggaaccggt cggcccgatg agcaggatgt
     1141 tgctcttcgc cagttcgact tcgtcccggc cccccagaac ttcgacgcgc ttgtagtggt
     1201 tgtagaccgc gaccgaaagc accttcttcg cgtctttctg cccgatgacg tactgatcga
     1261 ggatcgcctt gatttccttc ggccgcggaa gtttcgaggc ggcggattcg ttgcgcccct
     1321 gcatctcctc gcggatgatc tcgttgcaga gttcgacgca ctcgtcgcag atatagaccg
     1381 acgggccggc gatgagcttg ccgacctcgt gctggctctt gttgcagaag gtacagaaga
     1441 gaaccttgcc gccgtcgcgc ggacctcgcg gttcttcgtt catcgcttca gctccggcga
     1501 tcgctcgcct gcccccgttg cgcccatatt tctaacgcag ggagcagggc tgcggcaagc
     1561 cccctcccct gcggcggccc ctctgcgaaa cgccgtcgcc gccgccatca ggggctgcgc
     1621 tccttcgccg ccgcgccccg attcttgaga atgctgtcga gaagcccgta ctcgagcgcc
     1681 tcgtctgcgc tcaggaaacg gtcgcgatcg gtgtcggtct gcaccttctc gaccggctgg
     1741 ccggtatgct cggcaagaat ctggttcagg cgtccgcgaa tcgtgagaat ctcgcgtgcg
     1801 tgaatctcga tgtcggattg ctggccctgc accccgccca gcggctggtg aatcatcatg
     1861 cgcgaatgcg gcagacagta gcgcttgccc ttggcgccgc cggcgaggag caccgcggcc
     1921 atgctcgccg cctgcccgag gcagatcgtg gagacatcgg gcttcacgaa ctgcatggtg
     1981 tcgtagatcg aaagtccggc cgtcaccgag ccacccggag agttgatgta aaggctgatg
     2041 tccttgtcgg gattctcgga ttcgagaaac agcagttggg cgacgacgag gttcgcgatg
     2101 tgatcctcga tcgccccgac cacgaacacc acccgctcct tgagcaggcg cgaatagatg
     2161 tcgtaggcgc gctcgccccg cggcgtctgc tcgacgacga tcggaaccag tgcctgcccg
     2221 gagatttcgc tcattccttg ctcctgtcgc ggccaccctc cggcggctcc atgaattcga
     2281 aaaaggatcg gggctcgtcg ctcaccgcgg cgcgtcccag caaccagttg taggccatgt
     2341 cttcgagcac gagcgcctcg acctggcgca tggcgtcgtt gtcggcgcgg atcttgcgcg
     2401 cctcggcgcc cgggtcttcg gcgtcgcggg tgagttccgc gatccgtctc tcgaccagcg
     2461 ccggatcggg gcggaaggct tcgcgttcaa tcagcgagga aagaatcagg ccggtctgca
     2521 cccgtcgctg cgccgtggct tcgaagggtt cgcgcggaat cgaggatacc tgccgcggct
     2581 cgggagtgat gccgaggcgg cgcagcgccg cacgcttctg acgcgcgatt tcgtcctcga
     2641 gcagggccgt cggcacatcg accgggttgg cggcgacgag ttgatcgctg acctggcgca
     2701 ggagttcggc gcgaacccgc tcctcggctt cgcccgccat gttcgtgcgt atccgcgacc
     2761 ggagttcggc gaccccgcct tccgcgactc cgaactcttc ggcaaacgcg tcatcgagtg
     2821 cgggaacttc cagttcctcg acccggtgga caagcacatc aaaatgcgcc ggccggccgg
     2881 cgagttccgt tcggcgataa ccccgcggga aagcaagatc gaagccccgg ctttccccgg
     2941 cgccgatgcc ctccagcgcc gcctcgaaac cgggaagcgt atcgccggaa ccgagcacga
     3001 gagcgagttc cttggcactg ttgccttcga aggcttcgcc gtcgacgcgc ccgtcgaaat
     3061 cgagaatgag gcgatcgccg gagcgcgccg gccgttccgc cgggatccag cggccccgtt
     3121 gccgccgcag tcttcccagc acttcctcca cgtcggcgtc gccgatctcg acctttgggc
     3181 gcagcaactt caaatcgtcc aggccctgcg gatcgagttg cggatagacc tccagatcgg
     3241 cggtgaaagt atagcttccg tccggcgccg tttcgccgcc cgcgaaacgc gggtttccca
     3301 ccgggatcag acttttttcg ctcaccgcgt cgctgtagtg actgcgccgc agctcctcca
     3361 gcacgtcctc gcgcaccgcg gattcatact gttggcgcac gatcttcgcc ggcgccttgc
     3421 ccggacgaaa gcctttgatt ttggcccgct tgccgattcc ctgcaggcgc cgaacgatct
     3481 cggcctcgat gctttcgccg ggaatggtga cgctgagccg gcgcttgaga gcgccctgtt
     3541 cctcgaccga tacctgcatg ccgtccgtcc accgtagccg cacgaaaaag aactggtgcg
     3601 aaaggagaga ctcgaactct cacgggggat tgccccaccg gctcctaagg ccggcgcgtc
     3661 taccgttccg ccactttcgc ggccacgctc tcgctgcgcc gcccattata cgggcgactt
     3721 ccagccagcc tcgtgacggc gccgatccgc tgcttcaggc cgcttcgctc tcggcggcgg
     3781 ccttttgccg gcttcccggt ccttcgagca gagcctcgcg ggtcgcccgc agcaccagct
     3841 cgacttcttc gtcgccgatc gcgaaatgcg gggtgtaacg cagcgagtgc gcaccgccgt
     3901 gaatgacgcc gaccccgcgc aggcgcagat actcc
//
LOCUS       DASUJG010000034         4785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1479828, whole genome shotgun sequence.
ACCESSION   DASUJG010000034 DASUJG010000000
VERSION     DASUJG010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1479828"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..405
                     /gene="rplR"
                     /locus_tag="VFX38_00655"
     CDS             43..405
                     /gene="rplR"
                     /locus_tag="VFX38_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012071920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX5313405.1"
                     /translation="MRHKEQSRERRSRRTRANIRTAEAVRLAVHRTPRHMYAQLISPG
                     GATTLAAASTLDAELRESLKGKGTGNMAAAAAVGKLIAERGKGAGVSRVAFDRSGYKY
                     HGRVRALADAAREAGLEF"
     gene            415..915
                     /gene="rpsE"
                     /locus_tag="VFX38_00660"
     CDS             415..915
                     /gene="rpsE"
                     /locus_tag="VFX38_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX5313406.1"
                     /translation="MPKATEGTSDPLEKLVAVNRVAKVVKGGRQFGFTALMVVGDGAG
                     RVGFGYGKAREVPAAISKATEQARRRMVQVPLAEGTLYYPLEGHFGATKVYLQPASSG
                     TGVIAGGAMRAVFEVAGVRNVLAKSFGSRNPINVVRATMKALTSIRAPQHFAAKRGRP
                     LAEIRS"
     gene            921..1121
                     /gene="rpmD"
                     /locus_tag="VFX38_00665"
     CDS             921..1121
                     /gene="rpmD"
                     /locus_tag="VFX38_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014704963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX5313407.1"
                     /translation="MNAARKSGQLKVTLLKSTAGRLRVHRACARGLGLGKTHSSAVVA
                     DTAENRGMIEKISYLLRVEEVS"
     gene            1121..1561
                     /gene="rplO"
                     /locus_tag="VFX38_00670"
     CDS             1121..1561
                     /gene="rplO"
                     /locus_tag="VFX38_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008292019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX5313408.1"
                     /translation="MRLNEIRPAPGSHKQPIRVGRGIAAGRGKTAGRGTKGQHARTGG
                     LRKVGFEGGQMPLQRRLPRSGFASHMSETRLELRLGALGGFEGERVDLAGLKAAGLVP
                     AHIERVKIILNGAIERALIIADPQVVATRGARAAIEAAGGRIED"
     gene            1608..2891
                     /gene="secY"
                     /locus_tag="VFX38_00675"
     CDS             1608..2891
                     /gene="secY"
                     /locus_tag="VFX38_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005960946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX5313409.1"
                     /translation="MFRSQDLRKRLLYLAGALIVFRFGIHIPVPGIDPAAYARLFNQN
                     AGGILGIFNVFSGGGLQHMSIFALGVMPYISASIIIQMLTSVVPSLEAIKKEGQSGQR
                     KITRYTRYSTVFLALFQSIGAAFALQSQGVALDPGPSFIVVATVCLVTGTLFLMWLGE
                     QVTERGLGNGISMLIFAGIIAGLPSAIASTFNLVRNGELSSILAILIFALVILVTAFV
                     VFVESGQRRITVNYAKRQQGRRLYAAQTSHLPLKLNMSGVIPPIFASSIILFPVTLAG
                     WLGQSSGFGWLQSVALWLSPGQPLYVLLFAVLIIFFTFFYTALVFNSEETAENLKKSG
                     AFIPGIRPGKQTASYVDTVLTKLTLWGAVYLVAVCLLPEFLILYTHVPFNFGGTSLLI
                     IVVVAMDFMGQLQAHMMTHQYEGLLKRARLRGFQR"
     gene            2910..3023
                     /gene="rpmJ"
                     /locus_tag="VFX38_00680"
     CDS             2910..3023
                     /gene="rpmJ"
                     /locus_tag="VFX38_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEX5313410.1"
                     /translation="MKVRASVKRICRNCKIIRRRGAVRVICSDPRHKQRQG"
     gene            3100..3459
                     /gene="rpsM"
                     /locus_tag="VFX38_00685"
     CDS             3100..3459
                     /gene="rpsM"
                     /locus_tag="VFX38_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002690620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX5313411.1"
                     /translation="MARIAGINILPNKHTVIGLTAIFGVGRSRAAKICAAAGVDPARL
                     VKDLTDAELEGIRQEIGRYKVEGDLRRDVNMNVKRLMDLGTYRGVRHRRGLPVNGQRT
                     RTNARTRKGRVRRGAKR"
     gene            3525..3914
                     /gene="rpsK"
                     /locus_tag="VFX38_00690"
     CDS             3525..3914
                     /gene="rpsK"
                     /locus_tag="VFX38_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX5313412.1"
                     /translation="MAKPATKSRKRAKQQFVDAIVHVHASFNNTIITFTDRKGNTLSW
                     ATSGGSGFRGSRKSTPFAAQVAAERAGTAAQDYGVKNLEVEVKGPGPGRESAVRALNA
                     MGFHVLSISDVTPIPHNGCRPPKKRRV"
     gene            3929..4552
                     /gene="rpsD"
                     /locus_tag="VFX38_00695"
     CDS             3929..4552
                     /gene="rpsD"
                     /locus_tag="VFX38_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007161229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX5313413.1"
                     /translation="MARYLGPKCKLSRREGTDLFLKSPIKPLEKKCKLDRPPGQTGQA
                     RRKRPSDYGLQLREKQKLRRMYGVLERQFRRYYQEAARRRGATGELLLQLLESRLDNV
                     VYRMGFAATRAEARQLVNHKGVMVNGKVVNIPSYELAAGDEVGLTERARQQPRVATAR
                     EIASQIEPTRWVEVDEKQGRGIFKAVPERDEFLLDINENLVVELYSK"
     gene            4575..>4785
                     /locus_tag="VFX38_00700"
     CDS             4575..>4785
                     /locus_tag="VFX38_00700"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011070632.1"
                     /note="catalyzes the transcription of DNA into RNA using
                     the four ribonucleoside triphosphates as substrates.
                     Dimerization of the alpha subunit is the first step in the
                     sequential assembly of subunits to form the holoenzyme;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX5313414.1"
                     /translation="MDNFISEFLKPRIASVAEQGENRARVVLEPLERGFGQTLGNALR
                     RVLLSSMPGAAVIEVEIDGVLHEYTA"
BASE COUNT          869 a         1473 c         1594 g          849 t
ORIGIN      
        1 ctacgccgat gagcgcatcg tgctcaagga ggcgaagaag aaatgagaca caaggaacag
       61 tcgcgcgagc ggcgctcgcg gcgcacacgc gcgaatatcc gcaccgccga ggcggtgcgc
      121 ctcgcggtgc accgcacgcc gcgccacatg tatgcccagt tgatttcgcc gggcggggcg
      181 acgacgctcg cggctgcctc gacgctggat gcggaactgc gcgagtccct caagggcaag
      241 ggtacgggaa atatggcggc cgccgcggcg gtcggcaagc tcatcgccga gcgaggaaag
      301 ggcgcgggag tttctcgcgt ggccttcgac cgttcgggct acaaatatca cggtcgcgtt
      361 cgcgcgctcg ccgatgcggc gcgcgaggcg ggcctcgaat tttagggtag ctgcatgcca
      421 aaggcaacgg aaggaacgag cgatccgctg gagaaactgg tcgcggtcaa tcgggtcgcg
      481 aaggtcgtca agggcggccg ccagttcgga ttcaccgcgc tcatggtcgt gggcgacggc
      541 gcgggacgcg tgggtttcgg ctacgggaag gcccgcgaag taccggccgc gatttcgaag
      601 gcgaccgagc aggcccggcg ccgcatggtg caggtgccgc ttgcggaagg tacgctgtat
      661 tacccgctcg aagggcattt cggggctacc aaggtctatt tgcagccggc gtcttcgggc
      721 accggcgtaa tcgcgggcgg tgcgatgcgc gccgtcttcg aggttgcggg cgtgcgcaac
      781 gtgctggcca agagcttcgg ttcgcgcaac cccatcaacg tcgttcgcgc gaccatgaag
      841 gcgctcacgt cgattcgcgc gccgcagcat ttcgcggcca agcgcggccg gccactcgcg
      901 gaaatccgga gttgactgtg atgaacgccg cgaggaagtc cggtcaactc aaggttacgc
      961 tgctcaagag cacggcgggg cgcctgcggg tgcaccgggc ctgcgcgcgc ggcctcggtc
     1021 tcggcaagac gcattcgagc gccgtggtcg ccgataccgc cgagaatcgc ggcatgatcg
     1081 agaagatttc ctatctgctc cgggtcgagg aggtgtcctg atgcggctca acgagattcg
     1141 acctgcgccg ggcagccaca agcaaccgat tcgagtgggg cgtggcatcg ccgcgggccg
     1201 gggcaagacc gccggacgcg gcaccaaggg ccagcatgcc cgcaccggcg gcctgcgcaa
     1261 ggtcggcttc gagggcggac agatgccgct gcagcgccgc ctgccccgtt cgggtttcgc
     1321 ttcgcacatg agtgagacgc gcctcgaatt gcggctcgga gcgcttggcg gcttcgaggg
     1381 cgagcgcgtg gacctcgcgg gtctcaaggc cgcgggcctg gtcccggcgc atatcgagcg
     1441 cgtcaagatc attctgaacg gcgccatcga acgtgcgctc atcattgccg atccgcaggt
     1501 ggtcgcgacg cgcggcgcca gggcggcgat cgaggcggcc ggcgggagaa tcgaggactg
     1561 acatggcagc tccgggccaa caacaggctt tcagctcctt gggggacatg ttccgctcgc
     1621 aggatctgcg caagcgtctt ctctaccttg ccggcgcact gatcgtcttt cgcttcggga
     1681 tccacatccc cgtgccgggc atcgatcctg cggcctacgc acgcctgttc aaccagaatg
     1741 ccgggggcat ccttggtatc ttcaacgtgt tttcgggcgg cggcctccag cacatgtcga
     1801 tcttcgcgct gggggtcatg ccctacatct ccgcctcgat catcatccag atgctgacct
     1861 cggtggtgcc cagtctcgag gcgatcaaga aggaaggcca atccgggcag cgcaagatca
     1921 cgcgctacac gcgctattcc acggtgtttc tcgcgctctt tcagtcgatc ggggcggcat
     1981 tcgcgttgca aagtcagggc gtcgcgctcg acccgggtcc gagcttcatc gtcgtggcga
     2041 ccgtctgcct cgtgaccggc acccttttcc tcatgtggct tggcgagcag gttaccgaac
     2101 gcgggctcgg caacggcatt tccatgctga ttttcgccgg gatcatcgcc ggcctgccgt
     2161 cggcaatcgc gagcaccttc aatctggtgc gcaatggcga gctttcgtcc atcctcgcga
     2221 ttctgatctt cgcactcgtg attctcgtta cggccttcgt cgtattcgtc gaaagcggtc
     2281 agcggcgaat caccgtgaat tacgccaagc gccaacaggg gcggcgcctc tatgccgcgc
     2341 agacgagcca tctgccgctc aagctcaaca tgtcgggggt catcccgccg attttcgcct
     2401 cgagcatcat ccttttcccg gtgacgctcg ccggctggct gggacagagt tccggcttcg
     2461 gctggctgca atcggtggcg ctctggctgt cgcccggtca gccgctctac gtactgctct
     2521 ttgcggtgct catcatcttc ttcacgtttt tctacacggc acttgtcttc aacagcgagg
     2581 agacggccga gaacctcaag aaatcggggg ccttcattcc cggaatccgc ccgggcaagc
     2641 agaccgcctc ctatgtggat acggtgctca ccaaactcac gctgtggggc gccgtgtacc
     2701 tcgtggcggt atgcctgctt cccgagtttc tcattcttta cacgcatgtg ccgttcaact
     2761 tcggcggcac gtcgcttctg atcatcgtgg tcgtcgcgat ggacttcatg ggccaactcc
     2821 aggctcacat gatgacccat cagtacgaag ggttgttgaa gcgcgcccga ctgcgcggct
     2881 ttcaacgcta ggatcgcagg agagcaggaa tgaaggtacg ggcttcggtc aagcggatct
     2941 gccgcaactg caagatcatc cgtcgtcgcg gcgcggtgcg ggtgatctgt tcggatcctc
     3001 gccacaagca gcggcagggt tgagcggttt gatgatggac ggctcgcttg ctacaatacc
     3061 cggctgcctt gtgggcagtc gatcgctccg ggggcgctga tggcacgcat tgcgggaatc
     3121 aacattctgc cgaacaagca caccgtgatc gggctcacgg cgattttcgg cgtcggccgc
     3181 tcgcgggcgg caaaaatttg tgccgcggcc ggagtcgatc cggcgcggct ggtgaaggac
     3241 ctgaccgatg cggaactcga ggggatccgc caggagatcg ggcgctacaa ggtggaggga
     3301 gatctgcgcc gcgacgtgaa catgaacgtc aagcggctga tggatctcgg cacctaccgc
     3361 ggagtgcggc accgccgcgg gctgcccgtg aacggccaga gaacgcgaac caatgcgcgc
     3421 acgcgcaagg gacgcgtgcg gcgcggcgcg aagcgctaag caagagaaga gactggcgcc
     3481 ggcgattttg ccggtcacga ggagtttgag gaccaacagg aatcatggcg aaaccggcga
     3541 ccaaatcccg caagcgagcg aagcagcaat tcgtggacgc gatcgtgcat gtgcatgcat
     3601 cgttcaacaa cacgatcatc acctttaccg accgcaaggg caatacgctg tcctgggcga
     3661 cgtcgggagg ctcgggcttc cgcggctcgc gaaagtccac gccgttcgcg gcgcaggtgg
     3721 ccgccgagcg ggccggcacc gccgcgcagg actacggcgt caagaatctc gaggtcgaag
     3781 tcaagggccc ggggcccgga cgcgaatcgg ccgttcgcgc cttgaatgcg atgggtttcc
     3841 atgtgctgtc gatctccgac gtgacgccga ttccgcacaa cggttgtcgg ccgccgaaaa
     3901 agcgccgggt ctgagaggga ggagataaat ggcgcgatat ctcggtccga aatgcaagct
     3961 ttcgcggcgc gaaggcaccg atctttttct caagagcccg atcaagccgc tcgagaagaa
     4021 atgcaagctc gatcgtccgc cgggacagac gggtcaggcg cgacgcaagc gcccgtccga
     4081 ctacgggttg cagttgcgag aaaagcagaa actgcgccgg atgtacggcg tgttggagcg
     4141 tcaattccga cgctactacc aggaggccgc gcggcgtcgc ggcgccaccg gcgaactcct
     4201 gctgcagttg ctcgaatcgc ggctcgacaa cgtcgtctat cgcatgggct tcgccgcgac
     4261 ccgcgccgag gcccgccagc tcgtgaatca caagggcgtg atggtgaacg gcaaggtcgt
     4321 caatatcccg agttacgaac tcgcggccgg cgacgaggtg ggcctcaccg aacgcgcccg
     4381 gcaacagccg cgtgtcgcga ctgcgaggga aatcgcgagc cagatcgaac cgacccgttg
     4441 ggtcgaggtg gacgagaagc agggacgcgg catcttcaag gcggtgccgg aacgcgacga
     4501 gtttctcctc gacatcaacg agaacctggt cgtggagctt tattccaaat agtggatgag
     4561 gatttgacag gcgtatggac aatttcattt ccgaattttt gaaaccgcgt atcgcgagcg
     4621 tcgccgaaca gggcgagaat cgcgcgcgcg tcgtgctcga gcccctcgag cgcggtttcg
     4681 gccagaccct cggcaacgcg ctgcggcggg tgctgctttc ctccatgccg ggggcggcgg
     4741 tgatcgaggt ggaaatcgac ggcgtgttgc acgaatacac cgcaa
//
LOCUS       DASUJG010000035         3457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_745541, whole genome shotgun sequence.
ACCESSION   DASUJG010000035 DASUJG010000000
VERSION     DASUJG010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745541"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(347..811)
                     /locus_tag="VFX38_00705"
     CDS             complement(347..811)
                     /locus_tag="VFX38_00705"
                     /inference="COORDINATES: protein motif:HMM:NF015758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HEX5313415.1"
                     /translation="MNKSSASELDIAFELQERAAAVGFDWPDAAGPRAKIAEELAELE
                     EAAAPLLRDSREGKIPVEASASEEKVKEELGDLLFAVVNLARHLNVAPEAALAAANRK
                     FQRRFRYIEKRLAAESLRPDGVDLARLDALWNEAKQRERISKNSPIPNPQDR"
     gene            complement(808..1482)
                     /locus_tag="VFX38_00710"
     CDS             complement(808..1482)
                     /locus_tag="VFX38_00710"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX5313416.1"
                     /translation="MFEDTAGGVAGPAADEDRLAPRLQRLAQALEGNESRIWQPAFGR
                     LRRAGFLANAPLLPAAVLIVLLEQSGGTGILLTRRCDDLAAHPGQVAFPGGAQESGDR
                     DAEAAALREAEEEVALRPERVRILGRLPRYPTTTGYLVTPIVGHVAEAPTLRAQVGEV
                     ADIFVLPLPILLDSSRWEMRPLGVGAEPLLFPELAWKGQRIWGATAGMLQLLLPPLRA
                     AWNLGE"
     gene            1540..2931
                     /gene="purB"
                     /locus_tag="VFX38_00715"
     CDS             1540..2931
                     /gene="purB"
                     /locus_tag="VFX38_00715"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX5313417.1"
                     /translation="MTAGTDALFALSPLDGRYHPRTAPLRELLSEYGLIRARVRVELA
                     WFRALASAPGISECPALSSAAAARLDEIENGFDADAAAAIKSIEKETNHDVKAVEYFL
                     KERFAALPELAAAAEFLHFACTSEDINNLAWALVLDEARKGVLLPGLAAIRGSLERLA
                     RAEAATAMLARTHGQAATPTTLGKEIAVFAARIRRASARFEAVPLAGKLNGASGAYNA
                     HALAYPQLDWIALARTVVEGLGLDWQALTTQIEPHDRLAELLDALAGANTILIDLCRD
                     LWTYIGLGYFRQRLAPGEIGSSTMPHKVNPIDFENAEGNFGIANALARHLAATLPISR
                     LQRDLTDSTAQRALGSVFGHTLVALRSLERGLAKLEVAHERLAQDLDAHWEVLGEAAQ
                     TLMRKHGVAKPYETLKAATRGRTLDRAAWLALVRDLPLPAEARATLEALTPQRYTGLA
                     AELVRRHLGSDDD"
     gene            2924..3370
                     /locus_tag="VFX38_00720"
     CDS             2924..3370
                     /locus_tag="VFX38_00720"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5313418.1"
                     /translation="MTDFSIRLADWSDDLPALRDIRTRVFIEEQQVPLALEWDELDGQ
                     CVHALAVAGAEPVGTGRLTPDGHIGRMAVLREWRGRGVGAALLDILMHAARARGDHVC
                     RLHAQTHALGFYSRYGFLPEGEIFMEAGIPHQTMAFCFETATEQGR"
BASE COUNT          579 a         1231 c         1069 g          578 t
ORIGIN      
        1 aaccggaacg gaccggtaaa ccggaatgct cttcatgttc ccctccttga tgaaataccg
       61 gcgccctggc cccacgcccg ggcgcccccc gcgtcgcttc tccgaaacaa tggtgtgcca
      121 ccacgtccac gcagttgtca agcggccctg acggccttgc ccgtcacccc ggcgaaagcc
      181 ggggtccagt aagagtttgt atcccggcta tgagccggga ttcctcattt cactggatac
      241 cggcctgcgc cggtatgacg gaatcaaatc ggagattccg ttgccggggg ccggctcccg
      301 ggctgcgccg gtatgacgat ggagcgatgc ttgatcgacg ttgcctttat ctgtcttggg
      361 ggttcgggat cggggaattt ttcgagattc gctcccgctg cttggcctca ttccagaggg
      421 cgtcgagacg cgcaaggtca acgccatcgg ggcgcagact ctcggcggcg aggcgctttt
      481 cgatgtagcg gaaacggcgt tggaatttcc ggttggccgc ggcgagcgcc gcttccggcg
      541 ccacattcag gtggcgcgcc aggttgacga cggcaaacag caggtcgcca agttcctcct
      601 tcactttttc ttcgcttgcg gatgcttcca ccggaatctt gccttcgcga gaatcacgga
      661 gaagaggagc cgccgcttcc tccagttccg ccaattcttc cgcaatcttt gcgcgcggcc
      721 ccgcggcatc gggccagtcg aagccgaccg cggccgcgcg ttcctgaagc tcgaaggcga
      781 tgtcgagttc cgaggccgat gacttgttca ctcgccaagg ttccaggccg cgcgcagcgg
      841 cggaagcagc agttgcagca tgcccgcggt cgcgccccag attctctgcc ccttccaggc
      901 aagttcggga aacaacagcg gctctgcgcc gacaccgagc ggccgcattt cccagcggga
      961 ggaatcgaga agaatcggaa gcggcagaac gaagatgtcg gcgacttcac cgacttgcgc
     1021 gcgcagcgtc ggcgcttccg cgacatggcc gacgatcggc gtcaccagat aacccgtcgt
     1081 ggtcgggtag cgcggcagcc gccccagaat gcgcacgcgc tcgggacgca aggcgacttc
     1141 ttcttcggcc tcgcgcaggg cggcggcctc cgcatcccga tcgccgctct cctgcgcgcc
     1201 gcccggaaac gcgacctgcc ccggatgcgc cgcgagatcg tcgcaacgcc gcgtcaggag
     1261 aatgcccgtt ccaccgcttt gctccagcag tacgatcaac accgccgcgg gaagcaaggg
     1321 cgcattcgcg aggaaaccgg cgcggcgcag acgtccgaat gccggctgcc agatccgcga
     1381 ttcgttgccc tcgagcgcct gcgccagacg ttgcaagcgc ggcgccagcc gatcctcgtc
     1441 cgccgccggc cccgcgactc cgccagccgt gtcttcgaac atgccgatcc gtccgggagc
     1501 gaagtcccgt aaaataccca ttttagcggg cctcgggcca tgaccgccgg gaccgacgcg
     1561 ctgttcgcac tctcgccgct cgatgggcgt taccacccgc gaacggcgcc gctgcgcgaa
     1621 cttctttccg aatacggcct gattcgcgcg cgcgtgcgcg tcgaactcgc atggttccgc
     1681 gcacttgcct cggcacccgg catctccgaa tgtccggcgc tttcttctgc cgccgcggct
     1741 cggctcgacg aaatcgaaaa cggattcgac gcggacgccg ccgccgccat caagtccatc
     1801 gagaaagaaa ccaaccatga cgtgaaggcg gtcgagtact ttctcaagga gcgcttcgcc
     1861 gcgcttccgg aactcgccgc cgccgccgaa ttcctgcact tcgcctgcac ctcggaagac
     1921 atcaacaatc tcgcctgggc gctcgttctc gacgaggcgc gcaagggcgt tctgcttccg
     1981 gggctcgccg ccatccgcgg ctcactcgag agactcgcac gcgccgaagc cgcgacggcg
     2041 atgctcgcgc gcacccacgg acaggccgcg acgccgacca cgctcggcaa ggaaatcgcg
     2101 gttttcgcgg cgcgaatccg gcgcgcctcc gctcgcttcg aagcggtgcc gctcgccggc
     2161 aagctcaacg gcgcaagcgg cgcctacaac gcccacgcgc ttgcgtatcc gcagctcgat
     2221 tggatcgcac tcgcgcgcac ggtcgtcgag ggcctgggtc tcgactggca agcgctcacg
     2281 acccagatcg aaccccacga ccggctcgcc gaacttctcg atgcgctcgc cggcgcgaac
     2341 accatcctga tcgatctctg ccgcgatctc tggacctaca tcggcctcgg gtatttccgg
     2401 cagcgcctcg cgcccggcga aatcggctcc tcgaccatgc cgcacaaggt gaacccgatc
     2461 gactttgaga atgccgaagg caacttcggg atcgccaatg cgctcgcccg ccatctcgcc
     2521 gcgacgctcc cgatctcgcg cctgcagcgc gaccttacgg attcgaccgc gcagcgcgcg
     2581 ctcggctccg tcttcggaca taccctcgtc gccttgcgtt cgctcgaacg cggtcttgcg
     2641 aaactcgaag tcgcgcacga acgtctcgcg caggatctcg atgcgcactg ggaggtcctc
     2701 ggcgaagccg cgcagacgct catgcgcaag cacggcgtcg ccaagcccta cgaaaccctg
     2761 aaggcggcga ctcgcggccg aacgctcgat cgcgccgcct ggctcgcgct cgttcgcgat
     2821 ctgccgctgc cggcggaggc gcgcgcaacc ctggaggcgc tcacgccgca gcgctacacc
     2881 ggactcgccg cggaactcgt ccgccgccat ttgggcagcg acgatgactg acttttccat
     2941 ccggctcgcc gactggtcgg atgacctccc ggcgcttcgc gacatccgca cgcgggtttt
     3001 catcgaagaa cagcaggtgc cgctcgcgct cgaatgggac gaactcgacg gacaatgcgt
     3061 gcacgcgctg gccgtcgccg gcgcggagcc cgtcggcacc ggtcgcctca cgcccgacgg
     3121 acacattgga cgcatggcgg tactccgcga atggcgcggg cggggagtcg gcgcggcgct
     3181 cctcgatatc ctgatgcacg ccgcccgcgc gcgcggcgat cacgtctgtc ggctgcacgc
     3241 gcagacgcat gcgctgggct tctattcgcg ctacggattc ctgccggagg gtgagatatt
     3301 catggaggcc gggattccac accagacgat ggcgttctgc ttcgagactg caacggagca
     3361 gggccgatga accagcgcag tttccaaccg catgtcacgg tcgccgccgt cgccgaacgc
     3421 gacgggcgct ttctcgtcgt gcgcgaatac gtgaacg
//
LOCUS       DASUJG010000036         3152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_256258, whole genome shotgun sequence.
ACCESSION   DASUJG010000036 DASUJG010000000
VERSION     DASUJG010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256258"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="VFX38_00725"
     CDS             complement(<1..859)
                     /locus_tag="VFX38_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HEX5313419.1"
                     /translation="MPKRIEDYALIGNMRTAALVNREASMEWLCLPRFDSDACFAALL
                     GKPENGYWRIAPREESPAATRRYRGETLVLETVFETGGGAAAIMDFMALGETNDDVVH
                     VVRIVEGRKGRVAMDMTLVLRFDYGRIAPWMHGQKNGLCAFSGPDAVRLRTPVEIVNE
                     EGASRAEFTVKAGERIPFVLSHYPSYRKEPAECDAEAALEAAERWWTKWSGHCKIEGR
                     WREPAVRSLITLKALSDRETGGMVGAVTCGLPEKMGGSANWDYRYTWLRDTTFTLYAL
                     LASGFDEEAR"
     gene            1066..1788
                     /locus_tag="VFX38_00730"
     CDS             1066..1788
                     /locus_tag="VFX38_00730"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX5313420.1"
                     /translation="MTKFGASFVLALLIAGACAAAPGARAAANCTPRSVSVSGEGNAE
                     AAPGVYVFHLGISQRSTDVRSANAAVDKSAAAAVAAAKSAGLAKKDIQSTNLSISPVY
                     DSDAKPGEPQVYEVTRNLTLTLRDPSHYADLVEGLVKAGVNQITNIEARPADPQALAD
                     QALAAAVADARHKAALIAQGLDAKLGPALSLDENGGQMPRPVMMSAMKAAPSGAEAGY
                     EPGRITTQAQVSASFELEPSGC"
     gene            complement(1850..>3152)
                     /locus_tag="VFX38_00735"
     CDS             complement(1850..>3152)
                     /locus_tag="VFX38_00735"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5313421.1"
                     /translation="DGITEELTDALGQNAALRVIAWDTASGFRSLGLSAVEIGKRLDV
                     ASVLHGSIERMGDEVRVRAELVDATSGYQLWSAHYDASFENIFAVQDRITQAIAAALK
                     VKFAKSDLVAGGTRNPKAHELVLKGRALVNRLDNASLVAAQKDFEEAIALDPDYADAH
                     ALLGRALLLQTEASNQSLGDTRAKVRAEARRALALDPGNADALVELANVDESELDFAR
                     AREEYRRALALDPGNAAAHLDYSTVLPLTQAVAQAREAVLLDPQNSFAQNNLATYLLD
                     LGDFEEMLAPAHAIIRLDPRQVDAAFGLAYAYRQLHRYDDMVKAFDLVHPQTPLERRQ
                     VEAGRLTYRAAQDATLRPQALAALNRLRNSEPGPDAQLNLVQLYLALDEIGQARKLLD
                     EVCPAQPVDCSDLGVSPIYLPLRDDPGFQALAKKYTTITRE"
BASE COUNT          544 a         1040 c         1024 g          544 t
ORIGIN      
        1 cacgcgcctc ttcgtcgaat cccgaggcga gcagcgcata gagcgtgaag gtggtgtcgc
       61 gcagccaggt gtagcgataa tcccagttcg cactgccgcc cattttttcc ggcaggccgc
      121 aggtcaccgc gccgaccatg ccgccggttt cccgatcgct cagggccttg agcgtgatca
      181 gcgaacgcac ggcgggctcg cgccatctgc cctcgatctt gcagtggccg ctccatttcg
      241 tccaccaacg ctcggccgcc tcgagcgcag cctcggcgtc gcattcggcc ggctccttgc
      301 ggtacgacgg ataatgactc aacacgaagg ggatccgctc gcccgccttg accgtgaact
      361 cggcgcggct cgcaccttcc tcattcacga tctcgaccgg cgtgcgcagg cgcacggcat
      421 cggggcccga gaaagcgcag agtccattct tttggccgtg catccagggc gcgatccggc
      481 cgtaatcgaa gcgcaacacg agcgtcatgt ccatcgcgac gcgacccttt cgaccctcca
      541 cgatgcgaac gacgtggacg acgtcgtcgt tcgtctcgcc cagagccatg aaatccatga
      601 tcgccgcggc gccgccgccg gtctcgaaaa ccgtttcgag aaccagggtc tcgccgcgat
      661 aacgacgcgt tgcggcgggc gattcttcgc gcggcgcgat gcgccagtag ccgttttcgg
      721 gcttgccgag cagcgccgca aaacaggcgt cggaatcgaa acgcggcagg cacagccact
      781 ccatcgaagc ctcacggttg accagggcgg cggtgcgcat gttgccgatg agggcatagt
      841 cttcgatgcg tttgggcatg atttcccttc tccgattgcg ccggcgacat acgcaaccat
      901 agtgcgctca ttcgcgttcg atctgcaacg ccgcgtttct ccgccgaaga ggaacagctc
      961 ctgttgatgt cgctcttcgc gggaatcgct gctgcggcgg agcgcggatg acagaatgcc
     1021 ccgaagcgtg gcaccatgaa gtcctcattc atccggagca ccatcatgac gaaattcggc
     1081 gcttccttcg ttctcgccct gctgatcgcc ggcgcctgcg cggccgcgcc cggggctcgt
     1141 gccgccgcca attgcacgcc gcgcagcgtc agcgtttccg gtgagggcaa cgccgaggcc
     1201 gcgcccggcg tgtacgtgtt tcatctcggc atcagccagc gcagcaccga cgtgcgcagc
     1261 gccaacgcgg ccgtggacaa gtccgccgcc gccgcggtcg ccgccgccaa gtccgccggc
     1321 ctggccaaaa aggatatcca gagcaccaac ctttcgatca gccccgtgta cgactccgat
     1381 gccaagcccg gcgagccgca ggtgtacgaa gtcacccgca atttgacgct caccttgcgc
     1441 gatccctcgc attacgccga tctcgtcgag gggctcgtca aggcgggcgt gaaccagatc
     1501 acgaacatcg aggcgcggcc cgccgatccg caggcgctgg ccgaccaggc gcttgccgcg
     1561 gcggtcgccg acgcgcgcca caaggcggcg ctgatcgcgc aggggctgga cgccaagctg
     1621 ggtccggcgc tcagtctcga cgagaacgga gggcagatgc cgcgtccggt catgatgagt
     1681 gccatgaagg cggcgccctc gggcgccgag gccggttatg aacccggccg gatcacgacc
     1741 caggcgcagg tatcggcgag cttcgaactc gaaccttcgg gttgctgaac ctgcatcaca
     1801 acgggaaaaa cgatgagatt gcgtccgttg aacaaggcgg aggcggccgt tactcgcggg
     1861 tgatggtggt gtattttttc gcgagtgcct gaaaacccgg atcgtcccgc aggggaagat
     1921 agatcggact cacgcccaga tcggagcagt ccacgggctg ggcgggacag acctcgtcca
     1981 gcagttttcg ggcctgcccg atctcgtcga gggcgagata gagctgcacg agattgagtt
     2041 gcgcgtccgg tccgggttcg gaatttcgca gccggttcaa tgcggcgagt gcctgcgggc
     2101 gaagcgtcgc gtcctgcgcg gcacgatagg tgagccggcc cgcctcgacc tggcgccgct
     2161 cgagcggcgt ctgcgggtga acgagatcga aggccttcac catgtcgtcg tagcggtgca
     2221 actgccggta ggcgtacgcg aggccgaacg ccgcgtccac ctgccgcgga tcgagacgga
     2281 tgatggcatg agcgggcgcg agcatctcct cgaaatctcc caagtcgagg agataggtcg
     2341 cgagattgtt ctgggcgaac gagttttgcg gatcgaggag cacggcctcg cgcgcctgcg
     2401 cgacggcttg cgtgagcggg agcaccgtgc tgtagtccag gtgcgccgcg gcgttgcccg
     2461 gatcgagagc gagcgcacgg cggtactcct cgcgcgcccg tgcaaaatcg agttcgcttt
     2521 cgtccacgtt ggcgagttcc accagggcat cggcgttgcc gggatcgagc gcgagcgccc
     2581 gacgcgcctc ggcgcgaacc ttcgcgcggg tgtctccgag cgattgattc gaggcttcgg
     2641 tctgcagcaa cagcgcgcga ccgagcagag catgggcgtc ggcatagtcg ggatcgagcg
     2701 cgatcgcctc ttcgaaatcc ttttgcgcgg cgaccagact cgcgttgtcg agccgattca
     2761 cgagcgcacg gcctttcaac acgagttcgt gcgccttcgg attgcgggtg ccgccggcga
     2821 cgagatcgga tttcgcgaac ttgaccttga gcgccgcggc gatcgcctgc gtgatccggt
     2881 cctgcaccgc aaaaatattt tcgaaggaag catcgtagtg cgcggaccag agctgatagc
     2941 cgctcgtcgc gtccaccagt tccgcccgca cccgtacctc gtcgcccatg cgctcgatgc
     3001 ttccatggag aacgctcgcg acatcgagtc gtttgccgat ttccaccgcc gaaagcccga
     3061 ggctgcgaaa accggaggcg gtatcccagg cgatcacccg cagggccgca ttctgcccga
     3121 gcgcatcggt gagttcctcg gtgatgccgt cg
//
LOCUS       DASUJG010000037         2512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1724737, whole genome shotgun sequence.
ACCESSION   DASUJG010000037 DASUJG010000000
VERSION     DASUJG010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1724737"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1293
                     /locus_tag="VFX38_00740"
     CDS             <1..1293
                     /locus_tag="VFX38_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5313422.1"
                     /translation="AADWCRENAPAHMHFEIHRLPGRTPLLFMEIPGTTPPTADAPCV
                     LLYGHLDKQPEFEGWHEGLAPWEPVIRDGRLYGRGGADDGYAVFASLTAIRALQEQKV
                     PHARCVVMIELCEESGSFDLPHHFETLAERIGTPDLVICLDAECGNYEQFWCTTSLRG
                     NLVGTLSVEVLTEGVHSGAAGGIVPSSFRILRNLLDRVEDSATGTIKLAAFSVDVPNQ
                     RLEQARRSAEALGETSWRRFPFVEGAGPASRDPFNLLLANTWRACVEVTGAAGMPAPA
                     AAGNVLRPRTEIKLSFRLPPTADPGTAAAQAKEVLEDDPPMGTKVRFRVDSAMGGWDA
                     PPLAKWLEKAIREASQQHFGKEASYLGTGGSIPFMGMLGERFPEAQFLVTGLLGPQSN
                     AHGPNEFLHIATGKKLTACVADVLAAHAARDRSKSGKN"
     gene            complement(1349..1840)
                     /locus_tag="VFX38_00745"
     CDS             complement(1349..1840)
                     /locus_tag="VFX38_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313423.1"
                     /translation="MSGIALSRSLRSRLRRFLRDAACASRSSLVIPDGMDGFVHVATA
                     FVRPDGIYLLETLEGEGRLTAGERLPEWALSGKRRFHFPNPLTALELKVAAARLIAGA
                     IPLQGFLVLDDRLELPLACPPDVVVFAQLAERLPPLAADARPLPAYLEAWARFEAAGQ
                     AAS"
     gene            complement(1837..2145)
                     /locus_tag="VFX38_00750"
     CDS             complement(1837..2145)
                     /locus_tag="VFX38_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020413002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HEX5313424.1"
                     /translation="MNDRHFGSFRDFYPFYLSQHRNPVNRGLHCAGNTIVIALIVAAV
                     VTRRWWLLIALPLAGYGFAWLGHFFFEHNRPATFRHPLYSFLGDWIAFGETIVLRRRR
                     "
     gene            2255..>2512
                     /locus_tag="VFX38_00755"
     CDS             2255..>2512
                     /locus_tag="VFX38_00755"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX5313425.1"
                     /translation="MSKLADYAALILATMAERPERTHSASGIAAETGISLPTASKLLK
                     TLAHAGLVVSRRGARGGYSLGRAPAAISAVDMIEAVEGPLAM"
BASE COUNT          462 a          835 c          834 g          381 t
ORIGIN      
        1 gccgccgact ggtgccgcga aaacgcgccg gcacacatgc acttcgagat tcatcgcctg
       61 ccgggacgca cgccgctgct gttcatggag attccgggaa ccacgccgcc gaccgccgat
      121 gcaccctgcg tgctgctcta cggccatctc gacaaacaac cggaattcga gggctggcac
      181 gagggcctcg cgccctggga accggtgatt cgcgacggac gtctctacgg gcgcggcggc
      241 gccgacgacg gctatgcggt gttcgcctcg ctcaccgcga ttcgcgccct gcaggagcag
      301 aaggtcccgc acgcgcgctg cgtggtcatg atcgagctct gcgaggaaag cggcagcttc
      361 gatctgccgc accatttcga gacgctcgcc gagcggatcg gcacgccgga cctcgtgatt
      421 tgtctcgacg ccgagtgcgg aaactacgag cagttctggt gcaccacttc gctgcgcggc
      481 aatctggtgg gcacgctttc ggtcgaggtt ctcaccgaag gcgtccattc gggcgccgcg
      541 ggtggcatcg tcccttccag ttttcgcatc ctgcgaaatc tgctcgatcg cgtggaggat
      601 tcggcgacgg gcacgatcaa gctcgccgcc ttttcggtgg acgtgcccaa ccaacgcctc
      661 gagcaggcgc gccgctccgc cgaagccctc ggcgaaacct cctggcgccg cttcccgttt
      721 gtcgagggcg cgggaccggc gagccgcgat ccgttcaatc tgctgctcgc gaacacctgg
      781 cgcgcctgcg tcgaggtgac cggagccgcg ggtatgccgg cgccggcagc ggcgggaaac
      841 gtgttgcgcc cgcgcacgga aatcaagctt tccttccgcc tgccgccgac ggccgatcct
      901 gggacagccg ccgcgcaggc aaaggaagtg ctcgaagacg atccgccgat gggaacgaag
      961 gtgcgcttcc gcgtcgactc agccatgggc ggttgggatg caccgcccct cgccaaatgg
     1021 ctggagaagg cgattcggga agcctcgcag cagcatttcg gaaaagaggc gagctatctc
     1081 ggcaccggcg gcagcattcc gttcatgggc atgctgggcg agcgttttcc ggaagcgcag
     1141 tttctcgtca ccggtcttct cggaccgcag tcgaatgcgc acggacccaa cgagtttctc
     1201 cacatcgcga ccggcaagaa actcacggcc tgcgtcgccg acgtcctggc cgcgcatgcg
     1261 gctcgcgatc gctcgaagtc gggaaaaaac tgatccgcaa cgccacgctg ctctcgagca
     1321 ctgcgagaac gccgcgccgg cgtcgcgatc agctcgcggc ctgtcccgcc gcctcgaagc
     1381 gagcccaggc ttcgagataa gccggaagcg gccgggcgtc cgccgcaagc ggcggcagtc
     1441 tctcggccag ttgcgcgaag acgactacgt ccggcgggca ggcgagcggg agctcaaggc
     1501 gatcgtccag cacgagaaac ccctgcaagg gaatcgcgcc cgcgatcagt cgcgccgccg
     1561 cgaccttcaa ctcgagcgcg gtgagcggat tggggaaatg gaaacgtcgc ttgccgctga
     1621 gagcccactc cggaagcctc tcgccggccg tgagtcgccc ctcgccctcg agcgtttcga
     1681 ggagataaat gccgtccggg cggacgaagg ccgtcgcgac gtgcacgaag ccatccatgc
     1741 catcggggat cacgagactc gaacggctgg cgcaggccgc atcccgcaga aagcggcgca
     1801 gtcgcgagcg cagcgagcgg gacagtgcga tcccgctcat cggcggcgcc gtaggacgat
     1861 ggtttcgccg aaggcaatcc agtcgccgag aaaactgtag agcggatggc ggaaggtcgc
     1921 gggccgattg tgctcgaaaa agaaatggcc gagccaggca aagccgtagc cggcaagcgg
     1981 cagcgcgatc agcagccacc accgccgagt gacgaccgcc gcgacgatga gcgcgatgac
     2041 gatcgtgttg cccgcgcaat gaaggccgcg gttcaccgga ttgcggtgct gcgagaggta
     2101 aaacggatag aaatcccgga agcttccgaa atgccgatcg ttcaccgcaa aaatcgcgcc
     2161 tggggcaggt cagtatacgc agcgcttgcc gcccgggcct tatgggacta aaattgtgcc
     2221 atatggaaga tctgctggaa cgcccatgct gcgcatgagc aagctggccg actatgcggc
     2281 cctgattctc gcgacgatgg ccgagcggcc ggagcgcact cactccgcct ccggcattgc
     2341 ggccgaaacc ggcatcagcc tccccaccgc gagcaaactc ttgaaaaccc tcgcccatgc
     2401 cgggctcgtc gtgtcgcgcc gaggcgcccg tggcggctac agcctcgggc gtgcgcccgc
     2461 cgcgatcagc gcggtggaca tgatcgaggc ggtggaaggg ccgctggcga tg
//
LOCUS       DASUJG010000038         2482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_256595, whole genome shotgun sequence.
ACCESSION   DASUJG010000038 DASUJG010000000
VERSION     DASUJG010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256595"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..572)
                     /gene="grpE"
                     /locus_tag="VFX38_00760"
     CDS             complement(36..572)
                     /gene="grpE"
                     /locus_tag="VFX38_00760"
                     /inference="COORDINATES: protein motif:HMM:NF010737.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX5313426.1"
                     /translation="MSDDPLDEQTDGTPTSPPSISDRASEHSAARLATAEGQAAEERE
                     RALRLAAEMENLRRRTARDVEQARRYALERFAAELLPVADSLDLALGSAEAAPPNVRE
                     GLEMTLKLLHDIFARHHLEAIDPTGERFDPEFHEAIATQPTDQIAPDHVVTVVQKGFR
                     LHDRLLRPARVIVSRSPE"
     gene            complement(697..1746)
                     /locus_tag="VFX38_00765"
     CDS             complement(697..1746)
                     /locus_tag="VFX38_00765"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX5313427.1"
                     /translation="MTDVVQAAVLPTAKEAPGLHAGFWRRVAAYIIDAIALNIVIYIC
                     WIVVFLIFGLSSGGFSRAFVEPGRSPDFAWFAVFGVLEVATIVIMWLYFAVFESSRLQ
                     ATPGKLALGLGVTDGRGGRIGFGRATGRYFAKFLSSLIFCIGYLMAGWTERKQALHDI
                     LADTCVVQKFALVPNAADGNKAIGAHRVPGWVIVLIVAGTAVFYLAIFGIIIAIAAAV
                     ITKFNDRTPATETVDAMHQVRNFMVEFHERHGIWPPTRSIGQSISRMYSHSPSGDFKD
                     AVWIFRCSGKTCRIEARMRKTIANKALAGRTLELWTTDGGRSWHCGPGGSNPIPANGL
                     PPDCRDPGAPGPFPP"
     gene            complement(1788..>2482)
                     /locus_tag="VFX38_00770"
     CDS             complement(1788..>2482)
                     /locus_tag="VFX38_00770"
                     /inference="COORDINATES: protein motif:HMM:NF013768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HrcA family transcriptional regulator"
                     /protein_id="HEX5313428.1"
                     /translation="MAGLVTVPRRNRAAWRHIEFLRLSDRRVLAILVLDEHEVQNRVL
                     ELERDYSPELLARIAAYLNEHFGGRALGEVRSELLAAMREDIRNLNQLAQTALTMGER
                     MFTPEGESAADYVLAGETNLMGFADLADLERLKELFDAFTRKREILELLDRAIAAPGV
                     QVFIGEESGYAVLGELSVVVSSYGPEDRPLGVVGVIGPTRMAYQRVIPVVDVTARLLG
                     AALNSGDRGTVR"
BASE COUNT          459 a          816 c          790 g          417 t
ORIGIN      
        1 gaggttcgga ccccgctcgg caaggccttg cgccgctatt cgggcgagcg cgacacgatg
       61 acccgcgccg ggcgcagaag ccggtcgtgg agacggaagc ccttctgcac caccgtgacg
      121 acgtgatcgg gcgcgatctg atccgtcggc tgggtcgcga tcgcctcgtg aaactccgga
      181 tcgaagcgtt cgcccgtcgg gtcgatcgcc tcgaggtgat ggcgcgcgaa aatgtcatgg
      241 agcagcttga gcgtcatctc caggccctcg cgcacgttgg gcggcgccgc ctccgccgag
      301 cccagcgcga gatcgagact gtcggccacc ggcaggagtt cggcggcgaa gcgctcgagc
      361 gcatagcggc gcgcctgctc gacgtcgcgc gccgtgcgac gacgcagatt ctccatctcc
      421 gccgcgagtc gcagcgcccg ctcccgctcc tccgctgcct gcccctcggc cgtggccagt
      481 ctcgccgcgg aatgctccga agcccgatcc gaaatgctcg gcggcgacgt cggggtcccg
      541 tctgtctgct cgtcgagggg atcgtcactc atatcctggc tgctcaattg gacagtggaa
      601 tcgccgaacg catgggtgta cgcgattcgg cccgaaccgg gaacctggcg gcgacgctcc
      661 cgctgtcttg tctttctggg gacgatcgcc cgccattcaa ggcgggaacg gcccgggtgc
      721 cccaggatcg cggcagtcgg ggggaagccc gttcgcagga atggggttgc tcccaccggg
      781 tccgcaatgc caactccttc cgccatccgt cgtccacaac tccagggttc ttcccgcaag
      841 cgccttgttc gcgatggtct ttctcatcct cgcttcgatt cggcaagtct ttccgctgca
      901 acggaatatc cagacggcgt ccttgaagtc gccgctcggg gagtgcgagt acatgcggga
      961 aatggactgg ccgatgcttc gcgtgggagg ccaaatgccg tggcgttcgt gaaactccac
     1021 cataaaattc cgcacctggt gcatggcatc gacggtttcg gtggccggcg tccgatcatt
     1081 gaatttcgta ataaccgctg ccgcaattgc gatgatgatc ccgaatatcg ccagataaaa
     1141 gaccgcggtg cccgcgacga tgagcacgat tacccagcct ggtacccgat gcgccccgat
     1201 ggctttgttt ccgtccgcag cgtttggcac aagcgcgaat ttctgcacca cgcaggtatc
     1261 tgcgaggata tcgtgcaagg cctgcttgcg ctccgtccag cccgccatca ggtagccgat
     1321 gcagaaaatc aggctggaga ggaacttcgc gaaatagcgc ccggttgcgc ggccgaagcc
     1381 gatgcgcccg ccgcgcccgt cggttacgcc gagacccaac gccagcttgc ccggcgtggc
     1441 ctgcaaacgg gagctttcga aaacggcgaa gtacagccac atgatcacga tggtggcgac
     1501 ttccagaacg ccgaacacgg cgaaccaagc aaagtccggt gagcgcccgg gctcgacaaa
     1561 ggcgcgggag aacccgccgc tggatagtcc gaagatcagg aagacgacga tccagcaaat
     1621 gtagatgacg atgttgagtg cgattgcgtc gatgatgtag gcggcgacgc gtcgccagaa
     1681 ccccgcgtgc agaccggggg cctctttcgc cgtcggcaaa accgcggcct gaaccacgtc
     1741 cgtcatgaga accctccctc agccggatcg gcaattgcgc cggcagtcta tcgcaccgtt
     1801 ccacgatccc cggagttcag ggcggcgccg agcaggcgcg ccgtcacatc caccaccgga
     1861 atcacccgct gataggccat gcgcgtcgga ccgatgacgc cgaccacgcc gagcgggcgg
     1921 tcctcggggc cgtaactcga aacgacgacg gacagctccc cgagcaccgc gtagccagac
     1981 tcctcgccga taaagacctg cacgccgggc gccgcgatgg cgcgatcgag aagctccaga
     2041 atctcgcgct tgcgagtaaa ggcatcgaag agttccttga gccgttcgag atcggcgaga
     2101 tcggcgaaac ccatgagatt ggtctcgccg gcgagtacgt agtccgccgc cgattctccc
     2161 tccggcgtga acatgcgctc gcccatggtg agcgcggtct gggcgagctg attcagattg
     2221 cggatgtctt cgcgcatcgc ggcgaggagt tcggagcgaa cctcgccgag cgcccgcccg
     2281 ccgaagtgtt cattgagata ggcggcgatg cgcgcgagca gttccggcga atagtcccgc
     2341 tccagttcga gcacgcgatt ctgcacctcg tgctcgtcga gcaccaggat cgcgagcacg
     2401 cgccgatcgg aaagacggag aaattcgatg tgacgccagg ccgcgcgatt gcgtcgcgga
     2461 acggtgacga gccccgccat cc
//
LOCUS       DASUJG010000039         2772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_623128, whole genome shotgun sequence.
ACCESSION   DASUJG010000039 DASUJG010000000
VERSION     DASUJG010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623128"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..615)
                     /gene="rdgB"
                     /locus_tag="VFX38_00775"
     CDS             complement(4..615)
                     /gene="rdgB"
                     /locus_tag="VFX38_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018880975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEX5313429.1"
                     /translation="MRIVIATGNAGKLAEILTLLAPLGFDAIAQAALGIAPAAETAPS
                     FVENAILKARGAARAAHLPALADDSGLEVPALGGAPGVHSARYAGRHGDDAANVAKLL
                     AELAVRPGASRRARFRCVAVYLRAADDPAPIVAEATWEGEIAAAPRGKRGFGYDPVFL
                     LPALGITAAELPAAEKNRLSHRARALLALRERLAIERGESSPQ"
     gene            complement(603..1367)
                     /locus_tag="VFX38_00780"
     CDS             complement(603..1367)
                     /locus_tag="VFX38_00780"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX5313430.1"
                     /translation="MRFDTAQISLIGNRPENQDRTEILRAEGSLLAIVADGMGGHARG
                     ALAAETAVASLGASFRDDRSPRDPDEFLRAALACAHATVFALGKDKSFTQRPGTTAVC
                     ALVSGEELRWVHVGDSRAYHFRAARLLDRSRDHTRIESRLAAGEISPEAASLHPDRHV
                     VEYCLGVLEDSPPAEVSAARRLEPGDVVLLCSDGFWSQLDEERIGERLATGERLAPAI
                     RALAEEAASGANPHSDNVSAVALRVAADSQADPCGS"
     gene            1439..2308
                     /locus_tag="VFX38_00785"
     CDS             1439..2308
                     /locus_tag="VFX38_00785"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEX5313431.1"
                     /translation="MIRSMTGFARTTRRGDWGELTLELRAVNHRFLDVKLSLPDGLQG
                     LAEDLRAQLQKRLTRGRIEASVRWRPPAVAGFAINRELARAIAAELRSLQEEIGAAGE
                     APPLDLLRLLAWPGVVETATPESRLLSGELAAACDAALDELVAAREREGAALAGVLEE
                     RLQRLAAGLEACTAEERAAKVSLGERLAERIAALGAAADAARIAQEAAILVVRQDASE
                     ELERLAMHVREVRRLLRADEAAGRRLDFLMQELMREANTLASKAADVAVNRRALDFKV
                     LIEEMREQVQNVE"
     gene            2298..>2772
                     /gene="gmk"
                     /locus_tag="VFX38_00790"
     CDS             2298..>2772
                     /gene="gmk"
                     /locus_tag="VFX38_00790"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX5313432.1"
                     /translation="MSSDAAAGAGLYIVSAPSGAGKTSLLRALVERRPRLAFSVSYTT
                     REPRSDEIEGRDYHFIAEREFERMRGKGEFLECACVYGNGYGTGAADVLALRAAGRDV
                     VLEIDWQGAREVRSRVPESISVFILPPSRAVLRSRLTRRGTDSEQTITERMAVAAA"
BASE COUNT          398 a          925 c         1014 g          435 t
ORIGIN      
        1 gcgctattgc ggggagcttt cgccgcgctc gatcgcgagg cgttcgcgca gggcgagcaa
       61 ggcgcgagcg cggtgactca gccggttctt ttcggctgcc ggcaattcgg ccgccgtgat
      121 gccgagcgcc ggcagcagga agacggggtc gtagccgaaa ccacgcttgc cccgcggggc
      181 ggcggcgatt tcgccttccc aggtagcctc ggcgacgatc ggcgcggggt cgtcggcggc
      241 gcgcagatac accgcgacac aacggaagcg tgcgcggcga gacgctccgg ggcggaccgc
      301 gagttcggcg aggagtttcg ccacgttggc ggcatcgtct ccgtgccgcc cggcgtagcg
      361 cgccgaatgg acgcccggtg cgccgccgag cgccggaact tcgagcccgg agtcgtcggc
      421 cagggccgga agatgcgccg cccgcgccgc gccccgtgcc ttgaggatcg cgttttcgac
      481 gaagctcggg gccgtttctg cggcgggggc gatgccgagc gccgcttgcg cgatcgcatc
      541 gaagccgagc ggcgcgagca gagtcaggat ctcggcgagc ttgccggcgt tgccggtcgc
      601 gatcacgatc cgcacgggtc cgcctgcgaa tccgccgcca cccgaagcgc tacggccgaa
      661 acattgtcgc tgtgcgggtt cgcgccgctc gccgcctcct cggcgagggc gcggatcgcg
      721 ggcgcgaggc gctcgcccgt cgccagccgt tcgccgatgc gctcctcgtc caactggctc
      781 cagaatccat cggagcagag cagcaccacg tcgccgggtt cgagccgtcg tgccgcgctc
      841 acctccgccg gcggcgagtc ctcgaggacg ccgaggcaat attcgaccac gtgacggtcg
      901 ggatggaggc tcgccgcctc ggggctgatt tcgcccgccg cgaggcggct ttcgatccgc
      961 gtgtgatcgc ggctgcgatc gagaagacgc gccgcgcgga aatgatacgc gcggctgtcc
     1021 ccgacgtgaa cccagcgcaa ttcctcgccg ctcacgagcg cgcacacggc cgtggtgccc
     1081 ggacgttgcg taaacgattt gtcctttccc aaagcgaata ccgtcgcgtg cgcgcaggcg
     1141 agcgcggcgc ggagaaactc gtcgggatcc cgcgggctgc gatcgtcgcg gaaactcgcg
     1201 ccgaggctcg cgacggcggt ttcggcggcg agtgccccgc gtgcgtgtcc gcccatgccg
     1261 tcggcgacga ttgcgagcaa gctgccctcg gcacgcagaa tctccgtgcg atcctgattt
     1321 tcgggtcggt tgccgatgag gctgatttgg gcggtgtcga agcgcattgt cccgcgcgga
     1381 gttgacgttc gctgccgagt tccttagcgt agcatgcggt tttcaagcga ggatcggcgt
     1441 gattcgcagc atgacgggct tcgcgcggac gacgcgccgc ggcgattggg gcgaactcac
     1501 gctggaattg cgggcggtca accaccgctt tctcgacgtc aagctgagcc ttcccgacgg
     1561 cctgcagggt cttgccgagg atctgcgggc gcagctccag aagcgcctca ctcgcggacg
     1621 gatcgaggct tcggtacgct ggcgcccgcc ggcggtcgcg ggtttcgcca tcaaccgcga
     1681 actcgcgcgc gcgatcgccg cggaactgcg cagcttgcag gaggaaatcg gcgcggcggg
     1741 cgaggcccca ccgctcgatc tgctccggct gctcgcctgg ccgggggtcg tcgagaccgc
     1801 gacgccggag tcgcgtcttc tctccggcga gcttgccgcg gcttgcgatg ccgctctcga
     1861 cgaactggtc gcggcgcgcg agcgcgaggg agccgcgctc gcgggggtgc tcgaggagcg
     1921 cctgcaacgg ctcgccgccg ggctcgaagc ctgtacggcg gaggaacgcg cggccaaggt
     1981 gagtctgggc gaacggctcg ccgagcgcat cgccgcgctc ggagcggcgg ccgatgcggc
     2041 ccggatcgcg caggaggccg cgattctcgt cgtgcgtcag gatgcgagtg aagaactcga
     2101 acgactcgcg atgcatgtgc gcgaagttcg ccgtctgctc cgcgccgacg aagccgccgg
     2161 gcgccgcctc gactttctga tgcaggagtt gatgcgcgag gccaacacgc tcgcctccaa
     2221 ggccgcggat gtcgccgtca atcgccgcgc gctggacttc aaggtgctga tcgaagaaat
     2281 gcgcgagcag gttcagaatg tcgagtgatg cagcggcggg ggcggggctt tacatcgttt
     2341 ccgcgccgtc gggcgcaggc aagacttcgc ttctccgggc cctggtcgag cgccggccgc
     2401 gtctcgcctt ttccgtttcc tacacgaccc gcgagccgcg aagcgacgaa atcgaagggc
     2461 gcgactatca tttcatcgcg gagcgtgaat tcgagcgcat gcgcgggaag ggggaatttc
     2521 tcgaatgcgc ctgcgtgtac ggcaatggct acggcaccgg agcggcggac gtgctggcgc
     2581 tgcgcgcggc ggggcgcgac gtggtgctcg aaatcgactg gcagggggcg cgcgaggtcc
     2641 gctctcgcgt gccggagtcg atttccgtct tcatcttgcc gccgtcgcgc gcggtgctgc
     2701 gctcacgcct cacgcgccgc ggaacggaca gcgaacagac gatcacggag cggatggccg
     2761 tcgccgcggc cg
//
LOCUS       DASUJG010000040         3338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1235206, whole genome shotgun sequence.
ACCESSION   DASUJG010000040 DASUJG010000000
VERSION     DASUJG010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235206"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1677
                     /locus_tag="VFX38_00795"
     CDS             <1..1677
                     /locus_tag="VFX38_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5313433.1"
                     /translation="DLVLLPLAIGVFFLLAWASGHTPAGNYVGLRPTLSLDPKFLPLY
                     ALKTTLRMLCALAFSFLFTIVVGSWAAKSPRAEKIVIPLLDIGQSVPILGFLPFALLW
                     VVKGIPNPMVSANIAAIFAIFTSQVWNMAFSYYQSLRMLPKELKQAASVFQLSRWQRF
                     RRLEFPHALPQLVWNTMMSVSGGWFFVVAAEAITVSNHRVLLPGIGSYISVAIAHRDL
                     GAIGWALAAMFVVIMLYDQLAFRPLVAWAEKFKMHPSGGEEPQSWMLDLLRRSALVER
                     ARSGAQALARSLRRRRQARQDRPARERARIVRLTPRIGETTWNMLLAAIAVATVVLLG
                     RYIVAGVGPGEVGQVFLIGSYTLARVLVLTLLASLVWVPIGVAIGLRPKWTRRAQPLV
                     LFLSAFPANLLFPLFVIVILHWHLAPQIWLSPLMVLGAQWYILFNVIAGAQQIPGELR
                     EAADSLGLRGWRRWRRLYLPAVLPAYVTGGLTAAGAAWNASIVAEIVNWGGRTLKASG
                     LGSYITEQATAGDQPRLVLGIAVMIIYVLLMNRLLWARLYRLAEARFAET"
     gene            1679..2995
                     /locus_tag="VFX38_00800"
     CDS             1679..2995
                     /locus_tag="VFX38_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010958431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX5313434.1"
                     /translation="MAQTQNLIELRDVGKHFSGPERQRITVLDEVNFALREGEIVALL
                     GKSGSGKTTILRMIAGLVAPTSGEILYRGKPVRGCEPGISMVFQNFALFPWLDVLDNV
                     RLGPEAQGIERAEQRRRAIRAIDMIGLDGFESAYPKELSGGMRQRVGFARALVVEPDV
                     LLMDEPFSALDVPTAETLRDDLLDLWREKKIPTRAILMVSHSIEESLLLADRLLILDG
                     DPASIKSELPVRLAHPRDRNAPAFRGLVDRVYAAMTRPATAFLGAPSTAEVGLGYRLP
                     HANVQQMLGVLDAVCEESQGKRLELAELADELEMEVDEIFPPIEALELLGFATLDGGA
                     VHLTESGHAFLQADILQRKVLFGGVLMERVPLARRIRSEIESEADHRVDEERFLVALN
                     ESLSDEEAERVLEVVVAWGRYAEIFAYDYDSGIFSLENPSTEEAAR"
     gene            complement(3136..3336)
                     /gene="infA"
                     /locus_tag="VFX38_00805"
     CDS             complement(3136..3336)
                     /gene="infA"
                     /locus_tag="VFX38_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX5313435.1"
                     /translation="MQMEGRVVETLPNTTFRVELENGHVVTAHISGRMRKNYIRILTG
                     DKVTVELTPYDLTKGRIVFRGR"
BASE COUNT          513 a         1087 c         1091 g          647 t
ORIGIN      
        1 gatctggtgc tcctgccgct cgcgatcggc gtgttttttc tgctcgcctg ggcgagcggt
       61 catacgcctg ccggaaacta cgtcggcctg cgtccgacgc tttcgctcga ccccaagttt
      121 ctccccttgt atgcgctgaa gaccacgctg cgcatgctct gcgcgctcgc gttctcgttt
      181 ctcttcacca tcgtcgtcgg gagctgggcc gcgaagagcc cgcgggcgga aaagatcgtg
      241 attccgctcc tcgacattgg ccagtcggtg ccgatcctgg gctttcttcc cttcgcgctc
      301 ctgtgggtcg tcaagggcat ccccaatccg atggtgagcg cgaatatcgc ggcgatcttc
      361 gcgattttca cctcgcaggt ctggaacatg gccttcagct actaccagtc gctgcggatg
      421 ctgccgaagg aactcaaaca ggcggcgagc gtgttccagc tctcgcgctg gcagcgcttc
      481 cggcgtctcg agtttccgca cgcgctgccg caactggtgt ggaacacgat gatgtcggtg
      541 tcgggcggct ggtttttcgt ggtcgcggcc gaggcgatca cggtctccaa tcaccgcgtg
      601 ctgctgccgg gaatcggctc ctacatcagc gtcgcgatcg cgcatcgcga tctgggcgcc
      661 atcggctggg cgctcgcagc gatgttcgtc gtcatcatgc tctacgatca gctcgccttc
      721 cgcccgctcg tcgcctgggc ggaaaaattc aagatgcacc cctcgggagg cgaggaacca
      781 caatcctgga tgctcgatct cctgcgccgc agcgcgctgg tggaacgggc gcgctccgga
      841 gcacaggcgc tggctcgcag tctcaggcgt cgccgccagg cgcggcagga ccgaccggcc
      901 cgcgagcgcg cccgcatcgt gcgcctcacg ccgcggatcg gagagactac ctggaacatg
      961 cttctggcgg caatcgcggt cgcgaccgtg gtgctgctcg gtcgctacat cgtcgccggc
     1021 gtcggaccgg gggaggtcgg gcaggtattc ctcatcggct cctacacgct cgcgcgcgtt
     1081 ctggtcctca ctttgctcgc gagcctggta tgggtcccga tcggggtcgc gatcggtctg
     1141 cggccgaagt ggacccgacg ggcgcagccg ctggtgcttt ttctttcggc cttcccggcc
     1201 aatctcctgt tccccttgtt cgtgatcgtg attctgcact ggcatctcgc gccgcagatc
     1261 tggttgagcc cgctgatggt gctcggcgcg cagtggtaca ttctgttcaa tgtgatcgcc
     1321 ggtgcccagc agattccggg cgagcttcgc gaagccgccg attcgctcgg ccttcgcggc
     1381 tggcggcgct ggcgacgcct gtacctgccg gcggtgttgc cggcctacgt gaccggcggg
     1441 ctgacggcgg cgggggcggc ctggaacgcg agcatcgtcg ccgaaatcgt caactggggc
     1501 gggcgtacac tcaaggcgag cggcctcgga agctacatca ccgagcaggc gacggccggc
     1561 gaccagccgc ggctggtgct cggcatcgcg gtgatgatta tctacgtgct gctgatgaac
     1621 cggctgctct gggcgcgcct ctaccggctc gccgaggcac gttttgcgga gacttgatat
     1681 ggcacagaca cagaatctga tcgagctgcg cgacgtcggc aagcatttca gcggacccga
     1741 acggcagcgg atcacggtgc tcgacgaggt gaactttgcg ctgcgcgaag gcgagatcgt
     1801 cgcgctcctc ggcaaatcgg gttcgggcaa gacgacgatc ctgcgcatga tcgcggggct
     1861 cgtcgccccc acttcgggag agatcctcta ccgcggaaaa cccgtgcgcg gctgcgagcc
     1921 gggcatcagc atggtgtttc agaacttcgc gctttttccg tggctggacg tcctcgacaa
     1981 cgtgcgtctc ggcccggagg cgcagggaat cgagcgcgcc gagcagcgcc gccgcgcgat
     2041 ccgcgcgatc gacatgatcg gtctcgacgg tttcgagtcc gcctatccga aggagctttc
     2101 cggcggcatg cgccagcggg tcggcttcgc ccgtgccctg gtcgtcgagc ccgacgtgct
     2161 cctgatggac gagccgttct cggccctcga cgtgccgacg gcggaaaccc tgcgcgacga
     2221 tctgctcgat ctgtggcggg agaaaaagat tcccacccgc gcgatcctca tggtttcgca
     2281 cagcatcgag gagtcgctgc tgctcgccga tcggctgctg attctcgacg gcgatccggc
     2341 ctcgatcaaa tcggaactcc cggtgcgtct tgcgcatcct cgcgatcgca acgcccctgc
     2401 cttccgcggt ctcgtggatc gcgtctacgc cgcgatgacg cgcccggcca ccgcctttct
     2461 cggcgcgccg tcgactgcgg aagtcggtct cgggtaccgc ctgccgcatg ccaacgtgca
     2521 acagatgctc ggtgtcctcg atgcggtctg cgaggagtct cagggcaagc gtctcgagct
     2581 ggcggaactc gccgacgaac tggaaatgga ggtggacgaa atctttccgc cgatcgaggc
     2641 gttggaactc ctgggcttcg cgaccctgga cgggggggcg gtgcatctca ccgaatcggg
     2701 gcacgccttc ctccaggccg acatcctcca gcgcaaggtc ctcttcggcg gcgttctgat
     2761 ggagcgcgtg ccgctcgcgc ggcgcatccg ctccgagatc gaatccgagg ccgatcaccg
     2821 cgtggacgag gagcgattcc tcgttgcgct caacgagtcg ctgagcgacg aggaggccga
     2881 gcgggtgctg gaagtcgtgg tcgcatgggg ccgctacgcc gagatcttcg cctacgacta
     2941 cgacagcgga atattcagcc tcgaaaatcc ttccaccgaa gaggccgccc gttagccgga
     3001 gagtgcggac agattttgtc gaaaagtttt tgtaggcccg cataaggcga agctgttgcg
     3061 ggcatcttgc gtcgaatgcc ggcaacgccg ccacacggct tacgccggcc tacgggaaca
     3121 cgcgcagcga accgctcagc ggccgcgaaa gacgatgcgt cccttggtca gatcgtaggg
     3181 cgtgagttcg acggtcacct tgtcgccggt gaggatgcgg atgtaattct tgcgcattcg
     3241 ccccgaaata tgggccgtca ccacgtggcc gttctcgagt tcgacccgaa acgtggtgtt
     3301 cggcagggtc tcgacgaccc ggccttccat ctgcagac
//
LOCUS       DASUJG010000041         3545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_379886, whole genome shotgun sequence.
ACCESSION   DASUJG010000041 DASUJG010000000
VERSION     DASUJG010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379886"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..1072
                     /locus_tag="VFX38_00810"
     CDS             53..1072
                     /locus_tag="VFX38_00810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX5313436.1"
                     /translation="MSSRRRRALLLVLLAVAASMTVVFIVGGPKVFSSLPSIPAWVLC
                     TAPALMFLAWFANAGRVAILLHANGRRIGIVRAWLIAAGGDFGAALGPSNVTALAAYI
                     FLLGRTGLDSARSSALFMLEKVLDQMMFAVALITSAVVLVLNGRGAGHWTLFEVGLGL
                     SLGVLAFALLALVQFRRVVGSILWLLARLGLPRRQRRRIARWSFSFRRGVIAVASLPW
                     RHLALLAFCAAMYWAPRFAILPLVAAGLHADVPWAYLLAVQVLTIFAGQFSLLPGGTI
                     TVEAISAALLLPWVDRHLVGLMLLLWRASVFYFTLLIGCAGFLGATAWRSPCPRIEAS
                     APHPA"
     gene            1214..2008
                     /locus_tag="VFX38_00815"
     CDS             1214..2008
                     /locus_tag="VFX38_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313437.1"
                     /translation="MAKPNLEIPAERPLESLIDDLEPMVGDPGAPTLMVSFAGARLGM
                     GISKVALRKCIADVPVQVVITPDVFPWWWAEGGILGGAPDGMPSAKARAALQARWPHK
                     RLICEGTSFGATTALAAGSLFGAELVLAFAVLSFTGSLKRRYYRDSRWPENAKRTRMS
                     PAVRRTSLGTDLRRGLRNPGYKEALLIADETDRLDALHARRLLDLPRTGIRWEQAGGH
                     SVLRAMYLAGTLGPFLADAVAGRTPGAAAGAPSAAASMPAGQTHSA"
     gene            complement(2082..3341)
                     /locus_tag="VFX38_00820"
     CDS             complement(2082..3341)
                     /locus_tag="VFX38_00820"
                     /inference="COORDINATES: protein motif:HMM:NF014610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HEX5313438.1"
                     /translation="MTDTSRACNRIVIHYADIALKGRNRPRFEARLGRQIEWRLKRLA
                     LPWPVRRAGGRMAIEIGAERERIDEVLAFLGETPGIAIYFPAQWFPAKSGGRSEAEFL
                     RGPVPAALLALARSLHREGARFAVRVERHDPGLEVSGTALEAALGSLILAETPWEGVN
                     LSAPDRTFHIGLYPEGAFLSAERRRGLGGMPAGTAGRVVVLLSGGIDSPLAAFLMARR
                     GCAVDCLHFSAGYPSADEIAQAPPGRLAALLSRYAQTLRLHVLPYVEFDLALAGPTTG
                     HELLLFRRFMLRSAERLAQRSGASALVTGDSLSQVASQTLANLVSLDAAVAMPVFRPL
                     IGLNKPEIIAWAREIGSYPISIEAYKDCCALIARNPKTQSQPAHLEGVERERLGDYEA
                     LIEATFAQVLSLHFEAGERVAEPSASP"
BASE COUNT          529 a         1226 c         1163 g          627 t
ORIGIN      
        1 accgcgagct atgcatcctc tccaactaca ccgccgacaa gacaagccgg gcgtgagttc
       61 gcgtcggcgt cgcgcgcttc tgctcgtgtt gcttgccgtc gcggcgagca tgaccgtggt
      121 gttcatcgtc ggcgggccca aggtcttttc ctcgctgccg tcgattcccg catgggtgtt
      181 gtgcacggcg cccgcgctca tgtttctcgc atggtttgcg aacgccgggc gcgtcgcgat
      241 cctgctgcac gccaacggaa ggcgcatcgg catcgtacgc gcatggctga tcgcggccgg
      301 cggcgacttc ggcgcggccc tcgggccgag caacgtcacc gcactcgcgg cctatatctt
      361 tctcctcggg cgcacggggc tggattccgc acgctcgagc gcgctcttca tgctcgaaaa
      421 ggtgctggat cagatgatgt tcgccgtcgc gctcatcacc agcgccgtgg tactcgtgct
      481 caacggccgc ggcgccggcc actggactct gttcgaagtg ggactggggc tcagcctcgg
      541 ggtactcgcc ttcgccctgc tcgcgctcgt gcagtttcgg cgtgtcgtgg gctcgattct
      601 gtggttgctc gcgcgcctgg ggctgccgcg ccgccaacgc cggcgcatcg cgcgctggag
      661 tttcagtttt cgacgcggag tcatcgcggt ggcatccttg ccctggcgcc acctcgcgct
      721 gctcgccttc tgcgcggcga tgtattgggc gccccgcttc gcgatcctgc cgctggttgc
      781 cgcggggctg catgccgacg tgccctgggc gtatcttctt gcggtgcagg tgttgacgat
      841 ttttgccggg cagttctccc tccttcccgg cggcaccatc accgtcgagg ccatctcggc
      901 cgcactgctg ctgccctggg tcgaccgtca cctcgtgggt ctcatgctcc tcctgtggcg
      961 cgcgagcgtg ttctatttca cgctgctgat cggctgcgcc ggctttctcg gcgccaccgc
     1021 ctggcgcagc ccctgcccgc ggatcgaagc ttccgcaccg catccggcct gaggaaacca
     1081 gcgccgcacg gaacatgccg gcgcgacatt tcccttgcgc ctgcggctcg gaattcctta
     1141 caatttcgga ttgtttcggc tgcggcttcc gcttttgcgg gcgcggagca ccgaacggaa
     1201 aggactgagg gagatggcga aaccgaacct cgagataccc gcggaacgtc cgctcgagtc
     1261 cctgatcgac gatctcgaac cgatggtcgg cgaccccggc gctccgaccc tcatggtgtc
     1321 gttcgcgggt gcgcgcctcg gcatgggaat ctcgaaggtg gccttgcgca aatgcatcgc
     1381 cgacgtgccg gtgcaggtcg tgatcacgcc cgatgtgttt ccgtggtggt gggccgaagg
     1441 gggcattctc ggcggcgctc ccgacggaat gccgagcgcg aaggcgcgcg cagcgctgca
     1501 ggcgcgctgg ccacacaagc gtctcatctg cgaaggcact tcgttcggcg ccaccacggc
     1561 gctcgccgcc ggatcgctgt tcggtgcgga actcgttctc gccttcgccg tgctctcctt
     1621 caccggcagc ctcaagcggc gctactatcg cgattcgcgc tggccggaga acgccaagcg
     1681 aacgcggatg tcgccggcgg tgcgccgcac ttcgctcggt accgatctgc gccgcggcct
     1741 gcgcaacccc ggctacaaag aagcgctcct gatcgccgac gagaccgatc gcctggatgc
     1801 gctccacgca cgacgcctgc tcgaccttcc gcgcaccggc attcgctggg agcaggccgg
     1861 agggcacagc gtattgcgcg cgatgtatct cgccggcact ctcgggccgt ttctcgccga
     1921 cgccgtcgcc ggccgcacgc cgggagccgc cgccggcgct ccctccgctg ccgcaagcat
     1981 gccggccggg cagacccact ccgcctgatt cttcgggagc gaatccggat gggcgccgga
     2041 ttcgcgacat gaaaagaggc agggcggctt tcaacaggct gctagggaga cgcggaaggc
     2101 tctgccacac gttcgccggc ctcgaaatgc agcgacagta cctgggcaaa tgtggcctcg
     2161 atcagcgcct cgtaatcgcc gagacgttcc cgttcgaccc cttcgaggtg cgcgggctga
     2221 ctctgcgttt tcggattgcg ggcgatgagc gcgcagcagt ccttgtaggc ctcgatcgaa
     2281 atcgggtagc tgccgatctc gcgcgcccag gcgatgatct ccggcttgtt gagcccgatc
     2341 aacggccgaa agaccggcat ggcaacggcc gcgtccaggc tcaccagatt ggccagggtc
     2401 tgcgaggcaa cctggctgag gctgtcgccg gttacgagag cgctcgcccc cgatcgttgc
     2461 gccagacgct cggccgagcg cagcatgaaa cggcgaaaca gcagcagctc atgacccgtc
     2521 gtcgggccgg cgagcgcaag gtcgaattcc acatacggca acacgtgcag gcggagcgtc
     2581 tgcgcataac gcgagagtag cgccgcgagc cgccccggcg gcgcctgcgc gatttcgtcg
     2641 gcgctcgggt agccggcgct gaaatgaaga cagtccacgg cgcatccccg ccgcgccatg
     2701 agaaaggcgg cgagcgggga atcgatgcct cccgacagga gcacgacgac ccgcccggcg
     2761 gtccctgcgg gcatgccgcc gagtccgcga cggcgctcgg cggaaagaaa cgccccctcg
     2821 gggtagagtc cgatgtgaaa cgtacgatcg ggagccgaca ggttcacgcc ctcccagggg
     2881 gtttcggcga gaatcaggct gccgagcgcg gcctcgagtg cggttcccga cacctcgagc
     2941 cccggatcgt gacgctcgac gcgaacggca aaacgcgcgc cctcgcgatg gagcgagcga
     3001 gcgagcgcga gcagcgccgc cggtacggga ccgcggagga attcggcctc gctgcgaccc
     3061 ccgctcttcg ccggaaacca ctgcgccgga aaatagatcg cgatgccggg cgtttctccg
     3121 agaaaggcca gtacctcgtc gatccgctcg cgctcggccc cgatttcgat cgccatgcgc
     3181 ccgccggcgc gacgcacggg ccatgggaga gcgagtcgct tcaggcgcca ctcgatctgg
     3241 cggccgaggc gcgcctcgaa tcgcggccgg tttcttccct tgagcgcgat gtcggcgtag
     3301 tggatgacga tgcgattgca tgcacggctc gtatccgtca tgacctcctc ccccctcgcc
     3361 gccgcaccga tcgaagatgg tagcattcgc gacttccggc gaatccggct gatgcgcaca
     3421 ctcgtcccac gactcggact cggccgccag acctgggcct gggcgagcta cgatttcgcc
     3481 aattcggtgt tcgcgacgac cgtgatcgcg ggctttttcc cggtattcct cgatcagtac
     3541 tggcg
//
LOCUS       DASUJG010000042         2801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_623705, whole genome shotgun sequence.
ACCESSION   DASUJG010000042 DASUJG010000000
VERSION     DASUJG010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623705"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /gene="atpG"
                     /locus_tag="VFX38_00825"
     CDS             <1..784
                     /gene="atpG"
                     /locus_tag="VFX38_00825"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEX5313439.1"
                     /translation="AASKMRRAQERMAKARPYAERIRVVASHIAGALTEYRHPFLVER
                     EVKRVGIIVVATDRGLCGGLNVNLFRRSLQLAGEWREKGAGLSFGLVGAKAGAFFRHV
                     GGEVIAQADKLGDRPGMEQLIGLMTAMIGAFDEGRIDRLLIVENQFVNTMSQRPRVLQ
                     LLPIPPSERPAMGSGQWDYIYEPEAREVLDFTLRRYIESLIFQAVVENVACEMAARMV
                     AMKSASENAGELIEDLQLAYNKARQAAITREIAEIVGGAAAV"
     gene            959..2353
                     /gene="atpD"
                     /locus_tag="VFX38_00830"
     CDS             959..2353
                     /gene="atpD"
                     /locus_tag="VFX38_00830"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX5313440.1"
                     /translation="MSKEKTGKGIIVQIIGAVIDVEFPRASVPQIFHALVVEKVGLTL
                     EVQQQLGDGVVRAIAMGVSEGLERGLEVRDTGHPIRVPVGQATLGRIMNVLGEPVDEA
                     GPVQAEERWSIHRAAPGFEDQARSQELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKT
                     VNMLELIRNIAAEHSGLSVFAGVGERTREGNDFYHEMRDSKVIDKVAMVYGQMNEPPG
                     NRLRVALTGLTMAEYFRDEGRDVLMFIDNIYRFTLAGVEVSALLGRMPSAVGYQPTLA
                     EEMGKLQERITTTNKGSITSVQAVYVPADDLTDPSPATTFAHLDSTIVLSRQIAEQGI
                     YPAVDPLDSTSRQLDPNVIGQDHYDTARAVQNTLQRYKELRDIIASLGMDELSEEDKL
                     IVARARKIQRFLSQPFFVAEVFTGSPGKYVPLAETIRGFKGIVAGEYDALPEQAFYMV
                     GAIDEAVAKAKAAA"
     gene            2350..2775
                     /locus_tag="VFX38_00835"
     CDS             2350..2775
                     /locus_tag="VFX38_00835"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005002453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEX5313441.1"
                     /translation="MMATIHVNIVSATEAIFDGDAEMVFAPAEMGEVGILPRHAPLLT
                     RLRPGAVRVRIKEGEEREFFVTGGLLEVQPDEVSVLADTLMRAHDLDEQAALEARVRA
                     EENLAGKHGEIDLDRAREELIQAEAQLRMIEELRGKHRS"
BASE COUNT          512 a          887 c          933 g          469 t
ORIGIN      
        1 ggcggcgagc aagatgcgcc gcgcgcagga acgcatggcg aaggcccgcc cctacgccga
       61 gcgcatccgc gtggtggcga gccacatcgc aggggcgctg accgaatatc gtcatccgtt
      121 tctcgtcgag cgcgaggtga agagggtcgg tatcatcgtg gtcgcgaccg atcgcggact
      181 ctgcggcggc ctcaacgtca atctctttcg ccgcagcctg cagcttgcgg gcgaatggcg
      241 ggagaaggga gcgggacttt ccttcggcct cgtcggcgcg aaggccggtg ccttttttcg
      301 ccacgtcggc ggcgaggtca tcgcgcaggc cgacaagctc ggcgatcgcc cgggcatgga
      361 gcaattgatc ggcctcatga ccgcgatgat cggcgccttc gacgaaggcc gcatcgaccg
      421 gctgctcatc gtcgagaacc agttcgtcaa caccatgagc cagcgcccgc gggtgctgca
      481 gcttctgccg attccgccgt cggagcgccc ggcgatggga tcgggtcagt gggattacat
      541 ttacgagccg gaggcgcgcg aggtgctcga ctttacgctt cgccgctata tcgaatcgct
      601 gatcttccag gcagtcgtcg aaaacgtcgc ctgcgagatg gccgcgcgca tggtcgcgat
      661 gaagagcgcc tcggaaaacg cgggtgaact gatcgaggac ctgcagctcg cctacaacaa
      721 ggcacggcag gcggcgatca cccgcgagat cgcggagatc gtcggcggcg cggcggcggt
      781 atgacgtccc cccgcgcctg cggcgcgacc cccttcgttc cgaagggggt gagattcagg
      841 cccttccttg cgaagggcgg caactctttc ttccccctgc gcttgcgaag ggggattgag
      901 ggggatgtct attggagcga gcttgctcgc gatacaggtt tcagaattgg agaagacgtt
      961 gagtaaggaa aagacaggca aaggcatcat cgtgcagatc atcggcgccg tcatcgacgt
     1021 ggaatttccg cgtgcttcgg tcccgcagat tttccacgcg ctcgtcgtcg agaaggtcgg
     1081 gctcacactc gaggtgcaac agcagctcgg cgacggcgtg gtacgcgcga tcgcgatggg
     1141 ggtctcggaa gggctcgaac gcgggctcga ggttcgcgac accggccatc cgatccgtgt
     1201 gcctgtcggc caggcgacgc tcggacgcat catgaacgtg ctcggcgagc cggtggacga
     1261 ggccggcccc gtgcaggcgg aggagcgctg gtccattcac cgtgcggcgc cgggcttcga
     1321 ggaccaggcg cgcagccagg aactgctcga gaccggcatc aaggtcatcg atcttctctg
     1381 ccccttcgcc aagggcggca aggtgggtct attcggcggc gcgggcgtcg ggaagaccgt
     1441 caacatgctc gaactcatcc gcaatatcgc cgccgagcac tccggattgt cggtgtttgc
     1501 cggcgtcggc gagcgcaccc gcgagggcaa cgacttctat catgaaatgc gcgactcgaa
     1561 ggtcatcgac aaggtcgcga tggtctacgg gcagatgaac gagcccccgg gcaatcgcct
     1621 gcgggtcgcg ctcaccggcc tcaccatggc ggagtacttc cgagacgagg gtcgcgatgt
     1681 gctgatgttc atcgacaaca tctaccgctt caccctcgcc ggcgtcgagg tctcggcact
     1741 cctcggacgc atgccttcgg ccgtgggtta tcagccgacg ctcgccgagg aaatggggaa
     1801 gctccaggag cgcatcacca cgaccaacaa gggttcgatc acctcggtgc aggcggtgta
     1861 cgtgccggcg gacgatctga ccgatccgtc gcccgcaacc acgttcgcgc atctggattc
     1921 caccatcgtg ctttcgcgcc agatcgccga gcagggaatc tatccggcgg tggatccgct
     1981 cgattccacc agccgccagc tcgatccgaa cgtcatcggc caggatcatt acgacaccgc
     2041 ccgcgccgtg cagaacacgc tccagcgcta caaggaactg cgcgacatca tcgcgagtct
     2101 cggcatggac gagctttcgg aagaggacaa gctgatcgtc gcgcgggcgc ggaagatcca
     2161 gcgctttctc tcgcagccct tctttgtcgc cgaagtcttc accggctcgc ccgggaagta
     2221 cgtgccgctc gcggaaacga tccgcgggtt caagggcatc gtcgcgggcg aatacgacgc
     2281 gctccccgag caggccttct acatggtcgg cgccatcgac gaggcggtcg cgaaggccaa
     2341 agccgcagcc tgatggcgac catccacgtc aacatcgtga gtgccaccga ggccattttc
     2401 gacggcgacg ccgagatggt tttcgcgccc gccgagatgg gcgaggtcgg cattctgccg
     2461 cggcacgcac cgcttctcac gcggcttcgt cccggcgccg tgcgcgtgcg gatcaaggaa
     2521 ggagaggaac gcgagttctt cgtgaccgga gggcttctcg aagtgcagcc cgacgaggtt
     2581 tcggtgcttg cggacacctt aatgcgggcg cacgatctcg acgagcaggc ggcgctcgag
     2641 gcgcgcgtcc gcgccgaaga aaatctcgcg ggcaaacacg gcgaaatcga tctcgatcgc
     2701 gcgcgcgagg aactcatcca ggccgaggcg caattgcgca tgatcgagga gctgcgcggc
     2761 aaacaccgca gctaagagag ctgcccggtg cccgtcagca g
//
LOCUS       DASUJG010000043         4490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1481523, whole genome shotgun sequence.
ACCESSION   DASUJG010000043 DASUJG010000000
VERSION     DASUJG010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481523"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VFX38_00840"
     CDS             <1..513
                     /locus_tag="VFX38_00840"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX5313442.1"
                     /translation="VVDKNPLNFAHLGLIAALFPQARIVHCRRDPLDTALSIYFQNFA
                     HPRNDYAYRIEDIAAMTGFHRRIMTYWERVLPIPIYPIDYERFVAEPEAQRRSVIEWL
                     GLSWDELLLADSSAGAIHTASQWQARQPVYRHALGRARHYAPWIGPLSDALRSEGLAD
                     TGSAPRGSPE"
     gene            complement(446..1957)
                     /gene="dacB"
                     /locus_tag="VFX38_00845"
     CDS             complement(446..1957)
                     /gene="dacB"
                     /locus_tag="VFX38_00845"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEX5313443.1"
                     /translation="MSRPTYRKPARWRVAFAIFLLAASGTAFADGSLAPLVELVNDGA
                     RVSALVVRLDDGSELAAIAPDTPLAPASVSKLYVAAAALDRWGPDYQFPTRLLAAGKI
                     HDGTLEGDLIFAGLGDPGVTNLQLWQLAHNLVERGITHVTGNLVVSAGYFGPLDQCVT
                     KDRCEAAGESDHAYDALLSSAAVNYSTVGITVIPGAWPGAAARVALEPFSLPMFDLRN
                     RLKTSKSKDDWKLSVHRETSGGRDILTVSGSVPAGAKPVTFSRSVSDPNLYAGETLAA
                     FLAQAGVQVDGQVRVAPTGMPAGVVVAEVDGERMWTLLRNMLLWSNNFMADTFALDIL
                     RAQQAPPLSLTAAGAAVSSIGRALERASPLMRSHEPKVELLSGSGLTPTSRASARDIV
                     ALLDTLYHRGGLLPSFLGALSVPAYAPSRMLKAPGDPLWMERVAAKTGSLSEPHSVFA
                     LAGYLRLDDGSWGAFAVLVNGTVRHDVPLWIAIQATREALIPYLRAPRSARHR"
     gene            complement(2065..3411)
                     /gene="xseA"
                     /locus_tag="VFX38_00850"
     CDS             complement(2065..3411)
                     /gene="xseA"
                     /locus_tag="VFX38_00850"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018234127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX5313444.1"
                     /translation="MNDGPHRENPVFSVSDLVGAAKLLLEEGLGSVAVEGEVSNLSRP
                     RSGHVYFTLKDAGAQLRCALFQREALRVRFPLADGLKVLARGRLSIYPARGDFQLYVA
                     ELEEAGLGALQRAFEQLKQRLAEEGLFSAELKRPLPRFPRRIGVVTSATGAALRDILH
                     VLARRYPPARVLIYPTPVQGESAAASIVAALSRASARADCDVLILARGGGSLEDLWPF
                     NTEIVARAIRASRIPVITGVGHETDFTIADFAADCRAPTPSAAAETVVPDAAELQARL
                     AANETRLARAWRRAAQGHGQALRTLSRRLAAQHPRRRVQERAQRLDELAGRLTAAAAR
                     SLQIRGAGIAARRSDLARLSPRSRMHRDVLRLATLRLALAHAGGPGLERRRAGLAAIG
                     ARLQALGPEQTLARGYAIVFDAQGRVLRDARTVVQGARIRARLARGEIGATVEEVR"
     gene            3410..4168
                     /locus_tag="VFX38_00855"
     CDS             3410..4168
                     /locus_tag="VFX38_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313445.1"
                     /translation="MRASYAVAAAVRSDSGEGAEKWAKRVYASEENIAENWHVSVRSI
                     IPLAAGLILAAAAPRAFAFASPQGTPQDHAPPVVAAGAPTATKLSTITVSGVRIPFPV
                     ALQLYKKALTRPWSNDPADADKLVCHWQAELGTHLQTLQCMTNRAHQRRYQATQDALY
                     LGQSECGNDTVCLSNKVLFGGGMSGRLAGFTTRNSIQRSAMAPLLAKLPPANASYTLR
                     VMAHGKPLIDYVIKDGDLVHVYHYAYKNAKPDKP"
BASE COUNT          755 a         1458 c         1551 g          726 t
ORIGIN      
        1 gtcgtcgaca agaatccgct caatttcgcg catctcggat tgatcgccgc gctgtttccg
       61 caggcacgga ttgtgcattg ccgccgcgat ccgctcgaca ccgcgctctc gatctatttc
      121 cagaacttcg cgcatccgcg caacgactat gcctatcgca tcgaagatat cgcggcgatg
      181 acgggattcc atcggcgcat catgacgtat tgggagcggg tactgccgat tccgatctat
      241 ccgatcgact acgagcgctt cgtcgccgaa cccgaagcgc aacgcaggtc cgtgatcgag
      301 tggctcggac tttcgtggga tgagttgctt ctcgccgatt cgtccgccgg cgcgattcat
      361 accgcgagtc agtggcaggc gcgccagccg gtataccgtc atgcgctcgg gcgggcccgg
      421 cactacgcac cctggatcgg accgctcagc gatgccttgc ggagcgaggg gctcgcagat
      481 acgggatcag cgcctcgcgg gtcgcctgaa tagcgatcca cagcggcacg tcgtgccgca
      541 cggttccgtt caccagcacc gcgaaggcgc cccagcttcc gtcatccagc cgcaggtagc
      601 cggcgagcgc gaacacgctg tgcggttcgc tcagggaacc cgttttcgcg gcgacgcgct
      661 ccatccacag agggtcgccc ggcgccttga gcatccggct cggcgcgtag gcgggaaccg
      721 agagcgcacc gagaaaactc ggcagcaatc cgccgcggtg atagagcgtg tcgagcagcg
      781 cgacgatgtc gcgggcgctc gcgcgactcg tcggcgtaag tccgcttccg ctcagcagct
      841 cgaccttcgg ttcatgcgaa cgcatgagcg gggaagctct ctcgagcgct cgcccgatgc
      901 tgctcacggc ggcaccggcc gcggtgagac tcagcggcgg cgcctgctgc gcgcgcagga
      961 tgtcgagcgc gaaggtatcg gccatgaagt tgttgctcca cagaagcatg ttgcggagca
     1021 gagtccacat ccgctcgccg tccacttcgg cgacgacgac cccggcgggc atgccggtgg
     1081 gggcgacgcg cacctgtccg tccacctgca cgcccgcctg ggcgaggaag gcggcgagtg
     1141 tctctccggc atagagattc ggatcgctca ccgagcggct gaaggtaacg ggcttcgcgc
     1201 ccgccggaac ggagccgctg acggtgagga tgtcgcggcc gccgctcgtc tcgcgatgga
     1261 ccgagagttt ccagtcgtcc ttgctcttgg aggtcttgag gcgattgcgc agatcgaaca
     1321 tcggcagcga aaacggttcg agcgcgaccc gcgccgcggc gcccggccag gcgccgggga
     1381 tgacggtgat cccgacggtg ctgtagttga cggcggccga ggagagcagc gcgtcgtagg
     1441 cgtggtcgct ttcgccggcg gcctcgcagc gatccttggt gacgcattgg tcgagcggtc
     1501 cgaagtagcc ggcgctcacg acgagattgc ccgttacgtg cgtgatgccg cgttccacga
     1561 gattgtgcgc gagctgccag agctgaagat tcgtgacgcc gggatcgccg agcccggcga
     1621 agatgaggtc gccctcgagg gtgccgtcgt ggattttccc cgccgcgagc aggcgcgtgg
     1681 ggaactggta atccggcccc cagcgatcga gtgccgccgc cgccacgtag agtttgctca
     1741 ccgaggcggg agcgagcggc gtgtccggcg cgatcgctgc gagttcgctg ccatcgtcga
     1801 gccgcacgac cagcgcgctc acgcgcgcac catcgttcac cagttcgacc agcggagcga
     1861 gcgaaccgtc ggcgaaggcg gtcccgctcg ccgccagcag gaaaatcgcg aatgcgacgc
     1921 gccagcgtgc cggtttccgg tacgtaggtc gggacaagcg cgcgttcttc gcgcgttccc
     1981 ggcagccgac gcctgatgcc ggcaacggtc ttgcggcccg atgccggctc acaccggctg
     2041 gaggccattt gatcggacct tccctcaccg aacttcctca accgtcgcgc cgatttcgcc
     2101 gcgggcgagc cgggcccgga tgcgcgcgcc ctgcacaacg gtgcgggcgt cgcgaaggac
     2161 gcggccctgc gcatcgaaga cgatcgcata accgcgcgcg agggtctgct ccggtcccag
     2221 cgcctgcagg cgcgccccga tcgcggcgag tccggcgcga cggcgttcga gacctggccc
     2281 gcccgcatgc gcgagagcga ggcgcagcgt cgcgaggcgc aggacgtcgc gatgcattcg
     2341 cgagcgcggc gaaagacgtg cgagatccga gcgtcgtgcg gcgatgccgg ccccgcgaat
     2401 ttgcagcgaa cgcgcggccg cggccgtcag tcgtccggcg agttcgtcga gacgctgggc
     2461 ccgttcctgc acgcgccggc gcggatgctg cgcagcgagt cgtcgcgaga gggttcgaag
     2521 ggcctgtccg tgtccctgag cggcgcggcg ccaggcccgg gcgagccggg tttcgttggc
     2581 ggcaagtcgc gcctgcaatt ccgcggcatc cggtaccacc gtctcggccg ccgccgacgg
     2641 cgtgggggcg cggcagtcgg ccgcgaaatc ggcgatggtg aaatcggttt cgtggccgac
     2701 cccggtgatg acgggaattc tcgaggcccg gatcgcgcgc gcgacgattt ccgtgttgaa
     2761 cggccagaga tcctcgagcg atccgccgcc gcgcgcaagg atgagcacgt cgcaatcggc
     2821 gcgggcggag gctcgcgaca gggccgcgac gatcgacgcc gcggcgctct cgccctgtac
     2881 cggtgtcgga tagatgagca cgcgggccgg cgggtagcgc cgcgcgagca catgcaggat
     2941 gtcgcgcagc gccgcgcccg tggcggaggt gacgacgccg atgcgccgtg gaaagcgggg
     3001 aagcggtcgc ttcagctcgg cggaaaaaag cccttcttcg gcgagccgct gcttgagctg
     3061 ttcgaacgcg cgttgcaggg cgccgagtcc ggcctcttcc agctccgcga cataaagctg
     3121 gaaatcgccg cgggcgggat agatcgaaag ccgcccgcgg gcgaggacct tcagtccgtc
     3181 ggcgagcgga aagcgcacgc ggagcgcctc gcgctggaac agcgcgcagc ggagctgggc
     3241 cccggcatcc ttgagcgtga agtacacatg ccccgagcgg ggacgggaaa ggttggagac
     3301 ttcgccttcc accgcaacgc tgcccaggcc ttcctccagc aggagtttgg ccgcgccgac
     3361 caggtccgag accgaaaaga cggggttttc gcggtgcggg ccgtcattca tgcgcgcatc
     3421 ttacgccgtc gcggcggcag tccggtcaga ctcgggcgaa ggagcggaaa aatgggcgaa
     3481 acgggtctat gcttcagagg agaacatcgc ggagaattgg catgtttccg tacgctcgat
     3541 tattccgctg gccgccgggt tgatcctggc cgccgccgcc ccgcgggcgt tcgccttcgc
     3601 atcgccccaa ggcacgccgc aagaccacgc gccccccgtc gtggctgcgg gtgcgccaac
     3661 ggccacgaag ctcagtacca ttacggtcag tggtgtgcgc attccctttc ccgtcgcgct
     3721 ccagttgtac aagaaggcgc ttacgcgccc gtggtcgaat gaccccgcgg atgccgacaa
     3781 gctggtgtgc cactggcagg ccgaactggg cacccatctg caaactctcc aatgcatgac
     3841 gaaccgggct caccaacggc ggtaccaagc cacgcaagac gcactttatc ttggacaatc
     3901 ggagtgcggg aacgacaccg tctgtctttc caataaggtt ttgttcgggg gcgggatgtc
     3961 gggcaggttg gcaggattca cgacccggaa ttcgatccag cgaagcgcga tggcaccgtt
     4021 gcttgcgaag ctgccgccgg caaatgcgag ctacaccctg cgggtgatgg cccacggcaa
     4081 gccgctgatt gactacgtaa tcaaggacgg cgacctggtg cacgtctacc attacgctta
     4141 caagaacgcc aaaccggaca agccctgacg gattgctgaa cgatggcctg gcccggcgtc
     4201 ttgacggcag gagcgggctc gctcgcgatt atcggccttg aggatcgcga gcaagctcgc
     4261 tcctgcgatg gcttttcgtg attgtgaaga tgggaaatgg gtgcgcgtaa aatagccatc
     4321 gttccctggc ttggcgcacg agcgtggcac aaacggccga agaagagagc gcgcggtttc
     4381 cgcgcttcgt ttttgcgggt tccgggccgc aatgcgatgg cttgcgcgcc gcgatcaatc
     4441 gcgattttct cgccgacgag acggaagtcg tcgggcgcct gatcgaggag
//
LOCUS       DASUJG010000044         4486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_13319, whole genome shotgun sequence.
ACCESSION   DASUJG010000044 DASUJG010000000
VERSION     DASUJG010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13319"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VFX38_00860"
     CDS             <1..316
                     /locus_tag="VFX38_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016854168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX5313446.1"
                     /translation="LGDAPHVVYRSDSGLADYARHVAAADLFIGGSSGPLHIAGALDR
                     PTAAFYPRRRSASALRWQTLNSEGRRLSFMPPPDAGESDYDAIDPGAAISEMTKLLTT
                     TF"
     gene            complement(490..1293)
                     /locus_tag="VFX38_00865"
     CDS             complement(490..1293)
                     /locus_tag="VFX38_00865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005351490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5313447.1"
                     /translation="MRVLAIPGLSAVIIARDAADRLPPCIRALAFADEILVYDGGSAD
                     ATTQIAETLGARVAVDRDWQGFGRARQRAQDEARHEWILMVDSDERVTPALAQEIGAA
                     IDAGDPCTAYALARRTWVFGRYLAHGGWWPDRVTRLYHREHGRYDDALVHEKVLLAPG
                     TRLAKFASPLLHHSYRDMHEYLTKSADYAAAWAKTRERAGRDVSLAAGLAHGAGCFLR
                     MYVLRAGFLDGRAGFLLALLSAHSTFVKYADLWLRSHDPGPPEAGGISG"
     gene            1343..1615
                     /locus_tag="VFX38_00870"
     CDS             1343..1615
                     /locus_tag="VFX38_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313448.1"
                     /translation="MLKRLIGIVVTAAGVLVALVIGLGALLVLAFTALAAVCVFAIRS
                     RSWRQGRKRRPEVIEGEFEVMGNKADSARPADDVNSASAGRDDERR"
     gene            1599..2591
                     /gene="dusA"
                     /locus_tag="VFX38_00875"
     CDS             1599..2591
                     /gene="dusA"
                     /locus_tag="VFX38_00875"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEX5313449.1"
                     /translation="MTNDAEVLASRRLCVAPMMNRTDRHFRYLLRLLTRCTWLYTEML
                     VARALLHGDPRRLLRHDARERPLALQLGGYEPAVLANAARIAEAEGFDEINLNVGCPS
                     ARVRAGRMGACLMAEPALVGDCIAAMRAASSLPVTVKTRLGIDHHDDFPFLCGFSEAV
                     AAAGCGVLIVHARKAWLHGLDPKANRSVPTLDYGRVHEVKKHFPQLEVIINGGVNDLD
                     TAAELLGQVDGVMLGRAAYAKPLLLAEADARFYGLHRVAPALDEVLEGYLDYLGAIAA
                     AGERIGPALRHLPNLLSGLPATRALRRSLAQCAPHNDLRELARICAPLLRRQAA"
     gene            2663..3475
                     /locus_tag="VFX38_00880"
     CDS             2663..3475
                     /locus_tag="VFX38_00880"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020502917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5313450.1"
                     /translation="MKTRRKRSLAARADRYRLYERSVQDADAEAEFLKDNFRRVRGRA
                     ARSLREDFCGTARLACEWVLANPRHRAIGVDLDPEVLGWGREHNVARLSDAQRARLRL
                     VQADVREAPGRRLDLVAAMNFSYWIFRERETLAAYFASIRRALAPDGLFMLDAFGGSD
                     AYDLTRERRAFNGFTYIWEQAEFDPITARSLCYIHFRFRDGSRLNRAFSYEWRLWTLP
                     EIRELLAEAGFSRSTVLWQGTDEKTGDVDGDFVPAERGEPDPSWIAYVQAEP"
     gene            3472..>4486
                     /locus_tag="VFX38_00885"
     CDS             3472..>4486
                     /locus_tag="VFX38_00885"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF001209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEX5313451.1"
                     /translation="MRESSLEEPPAQSRVRLSAPLTLYRGGVLDEVEIAYETWGRLAK
                     NNLLLLFTGLSPSAHAASSQADPTPGWWEEMIGPGRAIDTNRWFVVCVNSLGSCFGST
                     GPASINPATGERWRLDFPLLAVEDIARGARATLEALGLPAPTAVIGSSLGGMSALAFA
                     LQFPRDAPNLLLISTAVHARPFAIAIRSMQREAIRRDPDWKDGNYAPRHEPETGMRLA
                     RKIGMLSYRSPDEWRERFGRARIRPEAPGPAADFRIAFEIESYLEAHAERFVGGFDAN
                     CYLYLSQAMDHFDAAEHGDGDLARAFSRLETKRALVIGVESDILFPLAQQQELASLLE
                     NSGA"
BASE COUNT          716 a         1513 c         1466 g          791 t
ORIGIN      
        1 actcggcgac gcgccgcacg tcgtgtatcg ctccgactcc gggcttgcgg attacgcccg
       61 ccatgtcgcc gccgcggatc tgttcatcgg cggctccagt ggtccgctgc acatcgccgg
      121 agcgctcgat cgcccgaccg cggcctttta tccgcgccgc cgttcggcct cggcgctgcg
      181 ctggcagacg ctgaacagcg agggaagaag actctcgttc atgcctccgc ccgatgcagg
      241 cgaaagcgac tacgacgcca tcgatcccgg tgcggcgatt tccgaaatga cgaaattgct
      301 gacgactact ttctgaaaag cccctcgtgc attgcatctg gcgtgcgccc ggggctcgaa
      361 agtcgagaag gggttgtagg ccggcataag cccatcgggc gttgccggca ttcggcgtcg
      421 aatgcccgca acgcttcgct tatgcgggcc ggcgtaccgg gcgttcggaa atcgcgagca
      481 agcttgctct tacccggata tcccgccggc ttcaggcggt cccggatcgt ggctgcgcag
      541 ccacaggtcg gcgtacttga cgaaggtcga atgcgccgag agcagcgcga gaagaaaacc
      601 cgcccgcccg tcgagaaagc ctgcgcgcag aacatacatg cgaagaaagc agcccgcgcc
      661 gtgcgcgagg cccgccgcga gcgaaacgtc gcgtccggcg cgttcgcggg ttttcgccca
      721 ggcggcggca tagtcggcgg acttcgtcag gtattcgtgc atgtcgcggt agctgtgatg
      781 cagcagcggg ctcgcgaact tcgcaagccg cgttcccggc gcgagcaaca ccttttcgtg
      841 aaccagggca tcatcgtaac gaccgtgctc gcgatggtag aggcgggtca cccggtcggg
      901 ccaccatcct ccatgcgcaa ggtagcgtcc gaagacccag gttcggcggg cgagggcgta
      961 agcggtgcag ggatcgccgg cgtcgattgc ggccccgatc tcctgcgcga gcgccggggt
     1021 cacgcgttcg tcgctgtcca ccatcaggat ccactcgtgg cgggcctcgt cctgcgcccg
     1081 ttggcgcgcc cggccaaaac cctgccagtc gcggtcgacg gcgacgcgtg cgccgagcgt
     1141 ttccgcgatc tgcgtcgtcg catcggcgct gccgccgtca tagacgagaa tctcgtccgc
     1201 gaacgcaagc gcccggatgc aggggggcag gcgatcggcc gcatccctgg caatgatgac
     1261 cgccgacagt ccgggaatgg ccaagacgcg caaccccttg cgttaccata tacattctac
     1321 gttcgttcgt cccctcccgc gtatgctcaa gcgcctgatc ggcatcgtgg tcaccgctgc
     1381 cggcgtgctc gtggcgctgg tgatcggctt gggtgcgctg ctggtgctgg cattcacggc
     1441 gttggccgcg gtatgcgtgt tcgcaattcg ctcccgcagc tggaggcaag gccgcaaacg
     1501 ccggcccgag gtcatcgagg gggaattcga ggtcatggga aacaaggcgg attccgcacg
     1561 ccctgccgac gacgtgaatt ccgcgagcgc gggtcgtgat gacgaacgac gctgaggtgc
     1621 tcgcatcgcg tcgcctttgc gtcgctccga tgatgaaccg caccgaccgg cacttccgct
     1681 atctgctgcg gctcctgacc cgctgcacct ggctgtacac ggagatgctc gtcgcccggg
     1741 cgttgttgca cggcgatccg cgacgcctgc tgcgtcacga tgcgcgcgag cggcccctgg
     1801 ccctgcagct cggagggtac gaacccgcgg tgctcgcaaa cgcggcccgg atcgccgagg
     1861 ccgaaggctt cgacgagatc aatctcaacg tgggctgtcc gagcgcgcgc gtgcgcgccg
     1921 ggcgcatggg cgcctgtctc atggccgaac cggcgctggt cggcgactgc attgccgcca
     1981 tgcgtgcggc ctcgtcgctg cccgtcacgg tcaagacccg tctcggcatc gaccaccacg
     2041 atgattttcc atttctttgc ggcttcagcg aagcggtcgc ggccgccggc tgcggcgtgc
     2101 tcatcgtgca tgcgcgcaag gcctggttgc acgggctcga tcccaaggcg aatcgcagcg
     2161 ttccgacgct cgattatgga cgggttcatg aagtgaagaa acattttccg cagctcgaag
     2221 tcatcatcaa cggcggtgtg aacgatctcg atacggcggc ggaattgctc gggcaagtgg
     2281 acggcgtgat gctcggccgt gcggcctacg cgaaaccgtt gctcctggcc gaagcggatg
     2341 cgagatttta cggattgcat cgcgtcgcgc ccgcgctgga cgaggtactc gaaggctatc
     2401 tcgactatct cggcgcgatc gccgccgccg gcgaacggat cgggcccgcc ctgcgccacc
     2461 ttcccaatct tctgagtggt cttcccgcaa cgcgagcact gcgccgcagc ctcgcgcagt
     2521 gcgcaccgca caacgatctg cgcgaactcg cgcggatctg cgcaccgctt ctgcgccgac
     2581 aggcggcctg agcgggcgct cgccacaaag tctcataaat cgtcaccccc gcgagatcgc
     2641 ggccacagga gcaaagcccc gcatgaagac cagacgcaag cgcagcctcg ccgcgcgcgc
     2701 cgatcgctat cggctctacg aacgatcggt gcaggatgcg gatgccgaag ccgagttcct
     2761 caaggacaac ttccggcggg tacgcgggcg cgcggcgcga agcctgcgcg aggacttctg
     2821 cggcaccgcg cgcctcgcct gcgagtgggt gctcgcgaat ccgcggcatc gcgcaatcgg
     2881 cgtggatctc gatcccgaag tgctcggctg gggccgcgag cataacgtgg cgcgcctgag
     2941 cgacgcgcaa cgcgcgcggc tgcggctcgt tcaggccgac gtgcgcgagg ccccgggacg
     3001 ccgcctcgac cttgtcgccg cgatgaactt cagctactgg atcttccgcg aacgcgagac
     3061 gctagcggcc tactttgcga gtattcgccg cgcgctcgct cccgacggcc ttttcatgct
     3121 cgacgccttc ggcggctcgg atgcctacga tctcacgcgc gagcggcgcg ccttcaacgg
     3181 cttcacctac atctgggagc aggcggagtt cgatccgatc acggcgcgtt ctctctgtta
     3241 cattcatttt cgttttcgcg acggttcgcg gctcaaccgg gcgttcagct acgaatggcg
     3301 actgtggacg ctgccggaga ttcgcgaact cctggccgag gccgggtttt cccgcagcac
     3361 cgtgttatgg cagggaaccg acgagaaaac cggagacgta gacggcgatt tcgttccggc
     3421 ggagcggggt gaacccgatc cctcctggat cgcctacgtg caggccgagc cgtgagggag
     3481 tcgtccctcg aggaaccgcc tgcgcagagt cgcgtgcgcc tgtcggcgcc gttgactttg
     3541 tatcgcggcg gcgttctcga cgaggtcgag atcgcctacg aaacctgggg acggctcgca
     3601 aagaataatc ttctcctcct gttcaccggc ctttccccga gtgcgcatgc cgcctcttcg
     3661 caggccgatc cgaccccggg ttggtgggag gagatgatcg ggcccgggcg cgcgatcgac
     3721 accaaccgct ggttcgtcgt ctgcgtcaat tcgctcggaa gctgtttcgg ctcgaccgga
     3781 ccggcttcga tcaacccggc cacgggcgaa cgctggcggc tcgactttcc gctgctcgcc
     3841 gtggaagaca tcgcgcgggg cgcgcgggcg acgctcgagg cgctcggcct gccggcgccg
     3901 acggccgtga tcggttcttc tctcggcggc atgtcggcgc ttgccttcgc gctccagttt
     3961 ccccgcgacg cccccaatct cttgctcatt tccacggcgg tgcacgcgcg gccgttcgcg
     4021 atcgcgatcc gctcgatgca gcgcgaggcc atccgccgcg accccgactg gaaggacggc
     4081 aactacgcgc cgcgacacga acccgagacg ggcatgcgcc tcgcccgcaa gatcggcatg
     4141 ttgtcgtatc gttctccgga cgaatggcgg gaacgcttcg ggcgtgcgcg gattcgcccg
     4201 gaggcgccgg gtccggcggc ggactttcgc atcgcgttcg aaatcgaaag ttatctcgaa
     4261 gcccacgccg aacgctttgt cggcggcttc gacgccaact gctatctgta tctttcgcag
     4321 gcgatggacc atttcgatgc ggccgaacac ggcgacggcg acctcgcacg cgccttctcg
     4381 cgcctcgaga cgaagcgggc gctcgtgatc ggcgtcgagt cggatattct ctttccgctt
     4441 gcgcagcaac aggaactcgc ctcgcttctc gaaaactccg gagcgc
//
LOCUS       DASUJG010000045         2493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_258096, whole genome shotgun sequence.
ACCESSION   DASUJG010000045 DASUJG010000000
VERSION     DASUJG010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258096"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1262
                     /locus_tag="VFX38_00890"
     CDS             <1..1262
                     /locus_tag="VFX38_00890"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5313452.1"
                     /translation="LRPITAADLAEARRRIAEAAPRLVLTDMRLPDGDGLGLVEWMRQ
                     QAPGIPVAVLTAYGNVETAVAALKAGAFDFLTKPLDLARLRRLVADALDLAPSASAGP
                     SPLLGEAPSMRQLRSEIARLARSQAPVLISGESGTGKELAARLIHASSARGSRPFVPV
                     NCGAIPAELMESEFFGHRRGSFTGAIADHAGLVASADGGTLFLDEVAELSPAMQAKLL
                     RVIQDRAVRALGATTETAVDVRFLSATHRDLARLVAEGKFRDDLYYRIDVIRLQLPPL
                     RERREDIPLLARSLLARIAAKEGLSTPALDESALAALAAHDYPGNVRELENVLERAFA
                     FATCGTLGAADIRLRRDPSAGEDEAAAETTLGARLSGLRREAIEEAIAAAGGKRGEAA
                     RRLGLTARQLRYRIAQLGIETVAHSGE"
     gene            1500..1988
                     /locus_tag="VFX38_00895"
     CDS             1500..1988
                     /locus_tag="VFX38_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019836160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEX5313453.1"
                     /translation="MKERQGGFTLIELMIVVAIIAILAAIAIPLYLNYTTRAQATGAV
                     SLTSGVKAGVVEYYNSNGDWPTSNAAAGAASSVSVVGKYVSKVQVNGPKGGRITATFC
                     NNGTGVVTTNGTITCEANKKLNGHTLTLSPTATEGSISWTCYVDDAKIFPLVPAECRH
                     VK"
     gene            2120..>2493
                     /locus_tag="VFX38_00900"
     CDS             2120..>2493
                     /locus_tag="VFX38_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313454.1"
                     /translation="MQIPIRARPWLPRLFLFALLGAAAAAYSSGLAGPFLLDDMANLP
                     ALGAWGPVNNAATLVTYLTSGIAGPTGRPLALASFLMDAHNWPANPWPFKFTGLLFHL
                     LNGVLLAALLVRLSRLRGLARET"
BASE COUNT          393 a          898 c          785 g          417 t
ORIGIN      
        1 ggcttcgccc gatcaccgcc gccgatctcg cggaggcccg ccgccggatc gcggaggccg
       61 cgccgcggct cgttctcacc gacatgcgcc ttcccgacgg cgacggcctc ggactcgtcg
      121 agtggatgcg ccagcaggcg ccgggaatcc cggtcgccgt gctcacggcc tacggcaacg
      181 tcgaaacggc cgttgccgcg ctcaaggccg gcgccttcga ctttctcacc aagccgctcg
      241 atctcgcgcg gctgcgccgc ctcgtcgccg atgcgctcga tctcgcgccg agcgcttcgg
      301 ccggcccgag cccgctcctc ggcgaagccc cttccatgcg gcaactgcga agcgagatcg
      361 cgcgcctcgc ccgcagtcag gcgccggtgc tcatcagcgg cgaatcgggc accggcaagg
      421 aactcgcggc ccgcctcatt cacgcatcga gtgcgcgcgg ttcgcgaccc ttcgtccccg
      481 tcaactgcgg ggcgattccc gccgaactca tggaaagcga attcttcggc catcgccgcg
      541 gaagtttcac cggcgccatc gccgatcacg ccggcctcgt cgcgagcgcc gacggcggca
      601 cgctctttct cgacgaggtc gccgaactct cgcccgcgat gcaggccaag ctgctgcgcg
      661 tcatccagga tcgcgcggtg cgcgcgctcg gcgcgacgac ggaaacggcg gtggacgtgc
      721 gctttctttc ggccacccat cgcgatctcg cgcgcctcgt cgccgagggc aagttccgcg
      781 acgatctcta ctaccgcatc gacgtgatcc gcctgcaact gccgccgctg cgcgagcggc
      841 gcgaagacat tccgcttctc gcgcggagcc tcctcgcgcg gatcgcggcg aaggagggcc
      901 tgagcacccc ggcgctcgac gagagcgcgc tcgccgcgct cgccgcgcac gactatccgg
      961 gcaacgtgcg cgaactcgaa aacgttctcg agcgggcctt cgcgttcgcg acttgcggga
     1021 cgctcggcgc cgccgacatc cggctgcgcc gcgatccgtc ggcgggcgaa gacgaagccg
     1081 cggccgagac tacgctcggc gcgcggcttt cggggctgcg ccgcgaggcg atcgaagagg
     1141 cgatcgccgc cgccggcggg aagcgcggcg aggcagcgcg gcgcctcggg ctcaccgccc
     1201 ggcaactgcg ttaccgcatc gcccagctcg ggatcgagac cgtcgcgcac agcggcgagt
     1261 gacaattttt gtccgcaagc gacaaaattt gccggtattt tttgctgcat tttcgggaat
     1321 atacctgaat cgaatgcccg ccggcctgcc ggcgtacgat cccaagtggt tgattcttcg
     1381 atgcaactcg ggggattccc ggccgggaat acgctcccct gccgcactgg cacggttttt
     1441 gctgctcttg ggctgtgggc ccgaaagccc acgacatccc aaacttatgg agagcggtaa
     1501 tgaaggaaag acaagggggc tttaccctga tcgagctgat gatcgtggtt gcaatcatcg
     1561 ccatcctggc ggcgatcgcg attcccctgt atctgaacta cacgacccgc gcgcaggcga
     1621 cgggcgccgt gtcgctcact tccggggtca aggccggcgt cgtcgagtac tacaacagca
     1681 acggagactg gccgacgagc aacgccgcgg ccggggcggc ctcgtccgtg agcgtcgtcg
     1741 gcaaatacgt aagcaaggtc caggtcaatg gacccaaggg cggtcggatc acggcgacat
     1801 tctgtaacaa cggcactggg gttgtgacga ccaacggaac catcacgtgc gaggccaaca
     1861 aaaagctcaa cgggcacacg ctgaccctct cgcccacggc gaccgagggc tcaatctcct
     1921 ggacctgcta cgtggacgac gccaaaatct tcccgctggt gcccgcggaa tgccggcacg
     1981 tgaagtgatt tgaccgagat cctcctccgg gcttgacctc ccggtatacg cgtacttggc
     2041 ccgctccggc gggccatttt tttgcccgta tgcgactccg atccgccggg aggaaacgac
     2101 gctgagtaga atgcccgcga tgcagattcc catccgcgcc cggccctggc ttccgcgcct
     2161 cttcctcttc gcgcttctcg gcgccgcggc cgcggcctat tcttccggtc tcgccgggcc
     2221 ctttcttctc gacgacatgg caaatctccc ggccctcggc gcctggggac cggtgaacaa
     2281 tgcggcgacg ctcgtgacgt atctcacgag cggcatcgcc ggaccgaccg gccgcccgct
     2341 cgcgctcgcc tccttcctca tggacgcgca caactggccg gcgaatccct ggcccttcaa
     2401 gttcaccggc ctgctgtttc atctcctgaa cggcgtgctg ctcgcggcgc tcctcgtccg
     2461 gctttcgcgg ctgcgggggc ttgcacgcga aac
//
LOCUS       DASUJG010000046         2012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1481678, whole genome shotgun sequence.
ACCESSION   DASUJG010000046 DASUJG010000000
VERSION     DASUJG010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481678"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..210)
                     /gene="yidD"
                     /locus_tag="VFX38_00905"
     CDS             complement(13..210)
                     /gene="yidD"
                     /locus_tag="VFX38_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014403550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX5313455.1"
                     /translation="MLGARCRFHPSCSSYARVAIARFGPLRGGILATWRILRCQPLCE
                     GGFDPVPESFTLRRCRSHGEH"
     gene            381..1400
                     /gene="dksA"
                     /locus_tag="VFX38_00910"
     CDS             381..1400
                     /gene="dksA"
                     /locus_tag="VFX38_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019336819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEX5313456.1"
                     /translation="MAKSKHGAARRSDKKAGHAAKSAKKARAAKSSAKPVHKAAPRPV
                     AKKARPAPTAKRKSVAAKKVTKSPAVKKSSKSPAVKKSTKTSIVKKSSKSSPVKKTSS
                     AAAAKKSKPQATKKTATSRAAKKAAVSRQSATPRAVSVPESPVVSNADVAAQESRHRS
                     APRKRVHQRPTSGIAARLSPGGGGGDEQTITREDGRYALPASVTIDLPKGYHPGAKEE
                     YMSPKQLAYFRQKLKDWRDQLVEESRQTMENLREEVRDVGDDAERATRETENSLELRT
                     RDRYRKLISKIEKALRKIEEGRYGYCEETDEEIGLERLEARPIATLSLDAQERREHLQ
                     KQMGD"
     gene            1833..>2012
                     /locus_tag="VFX38_00915"
     CDS             1833..>2012
                     /locus_tag="VFX38_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003183172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX5313457.1"
                     /translation="MTATVELPVLPLRDVVVYPHMVIPLFVGREKSMKALEAAMEGDR
                     RILLVAQKQPEIDDPG"
BASE COUNT          416 a          661 c          661 g          274 t
ORIGIN      
        1 ccagtcggcc attcagtgtt ctccatgcga gcggcagcgg cgcagcgtaa acgattcggg
       61 gacgggatcg aagccgccct cgcacaacgg ctggcacctg aggatccgcc aagtcgcgag
      121 gatcccgccg cgcaaggggc cgaagcgcgc gatcgcgacg cgggcgtagc tcgagcaact
      181 cggatggaag cggcagcgcg cgccgagcat cggactcagg aagcgcttgt agaacgtgat
      241 gagcaacagc aggacgcggg tcaaggctgt gcggcctaga atgaatggga ttgcgtgctg
      301 ggcgatgaaa tcggttgccc ggttgaaacg actacgctat aacacgcggt cgcatctcca
      361 caactttcga gtgcagggac atggccaagt cgaaacacgg cgccgccagg cgtagcgaca
      421 aaaaagcggg ccacgcagcc aagtcggcga agaaggcgcg tgcggcgaaa tcgtccgcca
      481 agccggtcca caaggccgcg cccaggccgg tggccaagaa ggcccggccg gcgccgacgg
      541 ccaagcgcaa gtccgtcgcc gcgaaaaagg tgaccaagtc gcccgccgtg aagaagtcgt
      601 ccaaatcgcc tgccgtgaag aaatcaacca aaacgtccat cgtgaagaag tcatccaaat
      661 catctcccgt gaagaagact tccagcgctg ccgcagcgaa gaagtccaag ccgcaggcca
      721 cgaagaagac cgccacgtcc cgtgcggcga agaaggccgc cgtgtcccgc cagtcggcga
      781 ccccgagggc cgtctccgtt cccgaatcgc cggtggtatc gaacgccgac gttgcggccc
      841 aggaatcgcg ccatcgttcc gcgccgcgca agcgtgtgca ccagcgtccc accagcggga
      901 ttgctgcgcg cctgagcccg ggtggtggtg gtggcgacga gcagacgatc acgcgggaag
      961 acggccgcta tgccttgccc gcgagcgtca cgatcgacct gccgaagggg tatcaccccg
     1021 gcgccaaaga ggaatacatg agcccgaagc agttggcgta ctttcgccag aagctgaagg
     1081 attggcgcga ccagctcgtc gaggaatcgc ggcagaccat ggagaatctc cgcgaggaag
     1141 tccgcgacgt gggcgacgac gccgaacgcg cgacccggga gaccgagaac tcgctggaac
     1201 tgcgtacccg cgaccgctac cgcaagctga tctccaagat cgaaaaggcg ctgcgcaaga
     1261 tcgaggaggg ccgctacggc tactgcgagg agaccgacga ggaaatcggc ctcgagcgcc
     1321 tcgaagcgcg tccgatcgcg accttgtcgc tggatgccca ggaacgccgc gagcatctgc
     1381 agaagcagat gggcgactag cgccgtatcg ctgctcgttc cacgggcgcg gtcgcgcgca
     1441 tggcgcacgc gacaatggac gccgatcgtt ccgatcgcgg gcgcggcccg ctcctgcaac
     1501 agccgctgtt gcccgctcct gcaacagccg ctgttgcccc ctcctgcaag agccgctgtt
     1561 gcaggagcgc gccatgcgcg cgaatggcgc tggtcccggc agctcaagac gttgggcttc
     1621 gctgcgctcg acccaacctg caaaagcgcc gacgccagaa cggatcgctc gccaagcgag
     1681 cggagccgct cccacgcgtg tttgcgagcc gcgtcaccct tgggcgcttg atccgggcgg
     1741 gcgccgccac cattctcaac ccatcgcccg cattacgggc ctgtgcgcaa tcctgacccc
     1801 ggatcgacat gccaaacgaa attcccgaaa ccctgaccgc gaccgtcgag ttgccggtgc
     1861 tgccgctgcg cgacgtggtc gtgtatccgc acatggtgat cccgctgttc gtcggccgcg
     1921 agaaatcgat gaaggcgctg gaggcggcga tggagggcga tcgccggatc ctgctcgttg
     1981 cgcagaaaca gccggagatc gacgatcccg gc
//
LOCUS       DASUJG010000047         3465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1359293, whole genome shotgun sequence.
ACCESSION   DASUJG010000047 DASUJG010000000
VERSION     DASUJG010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359293"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VFX38_00920"
     CDS             complement(<1..402)
                     /locus_tag="VFX38_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020160806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX5313458.1"
                     /translation="MNANDKAESRDFQAGVALADIPEGGMVAGHVGDAPVLLARVDGE
                     LRAIGAKCTHYGGPLGEGLVVGETVRCPWHHACFSLRTGEALGAPALDPVSCWKVEVT
                     DGRAFVREKASAAAPRRKPATSPESVVIVGGG"
     gene            633..1847
                     /locus_tag="VFX38_00925"
     CDS             633..1847
                     /locus_tag="VFX38_00925"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX5313459.1"
                     /translation="MNWDSLRQTVVNFTAHWESLAYVILILLVTAVVALAIHLVLRKR
                     CQRESTARHVWKDALLGALNAPLQGVVWMIGLSVAVDALTDGGRMSLLAEIFPPARDV
                     AVIAIAAWFLIRVVGRVEGNLFARARRHEQEFDATAAGAIGKLARAVIVVAAALVMMQ
                     SLGFSIASLLAFGGVAGIAIGFAAQTLVANLLGGLTVYVSRIFKVGEDIILPGTEVAG
                     TVQQIGWRATRVLGWNGKPFYVPNALFNTKTLVNHSRLERRSFSEYLLLRYRDFDKLQ
                     AIVRRANEVLAGREDLAYFVFRFDSFGDAALKLNLYAWPQSAPDGGFLPYAEFARIKE
                     EVLLAIAAIAVDEGCDLIQPNTHVYVRQGGGAELGEPARGAEPGHVLPPRARPGTHKQ
                     DAGKLIERREKA"
     gene            complement(1866..2915)
                     /locus_tag="VFX38_00930"
     CDS             complement(1866..2915)
                     /locus_tag="VFX38_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEX5313460.1"
                     /translation="MTWMPKPAVRHPALPKNALGLTRRDYEGRISTLCAGCGHDSISS
                     ALIQACYDLELPPHRIAKLSGIGCSAKTPTYFIGAAHGFNGVHGRMPSLATGAALANR
                     DLVYIGISGDGDTASIGLGQFVHAIRRGVNMLYMVENNGTYGLTKGQFSATADVGSTS
                     KGGEANPYAPIDLVRLALDMGAGFVARSFSGDKEQLVPLLRAALRYPGFAYIDCLSPC
                     VTFNNHEGSTKSYPYIREHGIEIADLVEPREEIEVSYAPGERKAVTLLDGSQWVLRKL
                     DGNYNPGDRRAALELLESHRRRGEIPTGLLYLDRDGKDMHAYLNTVATPLNALDFAAL
                     CPGSAALETINARLR"
     gene            complement(2912..>3465)
                     /locus_tag="VFX38_00935"
     CDS             complement(2912..>3465)
                     /locus_tag="VFX38_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEX5313461.1"
                     /translation="GDGIPYRTLPGTHPSRGAWFARGSGHDKYGRYTEDSDRYQDNVD
                     RLLRKFATAATLVPDSVVEAHPAGDAAVLYFGTTAEPVREALEALGARGVALDALRLR
                     AFPFTEKTRRFIEAHETVFVVEQNRDAQLRTLLLAECGFAPEKLVSVLHYGGLPVTAD
                     FLVASILEHLRPDVAIPVAGLAS"
BASE COUNT          577 a         1077 c         1193 g          618 t
ORIGIN      
        1 tccaccgccg acgatcacga ccgattccgg tgatgtcgcc ggcttgcgcc gcggcgcagc
       61 ggcggatgcc ttctcccgca cgaaggcgcg cccatcggtg acctcgacct tccagcagga
      121 caccggatcg agcgccgggg cgccgagtgc ctcgcccgtg cgcagactga aacaggcgtg
      181 atgccagggg cagcggacgg tttcgccgac gacgagtccc tcgccgagcg gcccgccgta
      241 atgcgtgcat ttcgcgccga ttgcgcggag ttctccgtcc acgcgcgcga gaagcacggg
      301 cgcatcaccg acgtggcccg cgaccatccc gccttccgga atgtcggcaa gcgccacccc
      361 ggcctgaaag tccctgcttt ccgctttgtc gttggcattc atcgcgaagt ctccttgcga
      421 ccgtccagca ccacgatgat actacggcca tgcgatccgc atggcgctcg cgtggcggca
      481 agggagccgt tgcggcacct ttctcccgtg cgcgcaaacc cggcggcggc gcgcgtttgc
      541 cgtgatgcac ggcgatgcgc gtttgcgcgg ccgcgtccgt ctgtgtttca atggaagcat
      601 ccaggaagcg gaaatatcac ggaggaatgc ccatgaactg ggattccctg cggcagactg
      661 ttgtcaattt tactgcccat tgggaaagtc tcgcctacgt cattctgatt ctgctcgtga
      721 ccgcggtggt tgcgctcgcg attcatctcg ttctgcgcaa acgctgccaa cgcgaaagca
      781 cggcccgtca cgtgtggaaa gacgccctgc tgggcgcgct caatgcgccc ttgcagggag
      841 tcgtgtggat gatcggtctc agcgtcgcgg tcgacgcgct gaccgatgga gggcggatgt
      901 cgctgctggc ggaaattttc ccgccggccc gcgatgtcgc ggtgatcgcg atcgccgcgt
      961 ggtttctgat ccgggtagtg ggccgcgtcg aggggaatct tttcgcgcga gcacgcaggc
     1021 acgaacagga attcgacgcg acggcggccg gcgccatcgg caagctcgcg cgcgccgtga
     1081 tcgtcgttgc ggcggcgctg gtgatgatgc agtcgctcgg cttctcgatc gcgagccttc
     1141 tcgccttcgg tggcgtcgcc ggcatcgcca tcggtttcgc ggcgcaaacg ctcgtcgcga
     1201 atctcctcgg tggtctgacg gtttacgtca gccggatctt caaggtcggc gaagacatca
     1261 ttctgcccgg caccgaagtg gctggcacgg tgcagcagat cgggtggcgg gcgacgcggg
     1321 tgctcggttg gaacggcaag cccttttacg ttcccaacgc gctgttcaat accaagacgc
     1381 tcgtcaacca ctcgcggctc gagcgccgct cgttttccga atacctgctg ctgcgttatc
     1441 gggacttcga caagctgcag gcgatcgtgc gcagggccaa cgaggtgctg gccgggcgcg
     1501 aggacctggc ctacttcgtg tttcgtttcg acagcttcgg tgacgccgcg ctgaaactga
     1561 acctctatgc ctggccgcaa tccgcaccgg atggcggatt tcttccctat gccgaattcg
     1621 cccgcatcaa ggaggaagtc ctgctggcga tcgccgccat cgccgtggac gagggctgcg
     1681 atttgatcca gccgaatacg cacgtctacg tgcgccaagg cgggggagcg gaattaggcg
     1741 agccggcgag aggcgccgaa cccggacacg tgctgccccc gcgggcgcgg ccgggaacgc
     1801 acaagcagga tgcgggcaag ctgatcgaac ggcgtgagaa ggcctgagaa agaacgtccc
     1861 gcggttcagc gcaggcgggc gttgatggtt tcgagcgcgg cggagccggg gcagagcgcc
     1921 gcgaaatcga gcgcgttgag cggggtcgcg accgtattca ggtaggcgtg catgtccttg
     1981 ccgtcgcgat cgagataaag gagtccggtc gggatttcgc cacgccggcg gtgcgactcg
     2041 agcaattcga gcgccgccct gcgatcgccc ggattgtaat tcccgtcgag cttgcgcagc
     2101 acccattgcg aaccgtcgag cagggtcacc gccttgcgtt cgcccggcgc gtagctcacc
     2161 tcgatttctt cgcggggctc gacgagatcg gcgatctcga tcccatgttc gcggatgtag
     2221 ggatagctct tggtcgaacc ctcgtgattg ttgaaggtga cgcagggaga aaggcaatcg
     2281 atgtaggcga agccgggata gcgcagagcg gcgcgcagca gcggcacgag ctgctccttg
     2341 tcaccggaga aacttcgcgc gacgaagccc gcgcccatgt cgagcgcaag ccgcacgagg
     2401 tcgatcggcg cgtaggggtt ggcctcgccg cccttgctgg tcgagccgac gtcggccgtc
     2461 gccgagaact ggcccttggt gagtccgtag gtgccgttgt tctcgaccat gtagagcatg
     2521 tttacgccgc ggcgaatcgc gtgcacgaac tggccgaggc cgatggaggc ggtgtcgccg
     2581 tcgccggata tgccgatata gacaagatcg cggttggcga gtgcggcgcc ggtcgcgagc
     2641 gagggcatgc gcccgtgcac gccgttgaag ccatgcgcgg caccgataaa atacgtcggc
     2701 gtcttcgccg agcagccgat gccggagagt ttggcgatgc ggtgcggcgg gagttcgaga
     2761 tcgtagcagg cctggatgag cgcggagctg atcgagtcgt ggccgcagcc ggcacaaagc
     2821 gtcgagatcc gtccttcgta gtcgcgccgc gtgagaccga gcgcattctt cggcagcgcc
     2881 ggatggcgca cggcgggttt cggcatccag gtcatgacgc gagccccgcg accgggatcg
     2941 ccacgtcggg tctcagatgt tcgagaatgc tcgcgacgag gaagtctgcg gtcaccggca
     3001 agccgccgta gtgcagcacg gagacgagct tctccggcgc gaatccgcat tcggcaagaa
     3061 gcagcgtgcg cagttgcgcg tcgcggttct gctcgacgac gaacaccgtc tcgtgtgcct
     3121 cgatgaaacg gcgagtcttc tccgtgaagg gaaaggcccg aaggcggagc gcatcgagcg
     3181 cgactccgcg tgccccgagt gcttcgagcg cttcgcgcac cggctcggcg gtggtgccga
     3241 agtagagcac cgcggcatcg cctgcgggat gtgcttcgac gacggagtcg ggaacgagcg
     3301 tcgccgccgt tgcaaatttg cgcagcagcc gatcgacgtt gtcctgatag cgatcgctgt
     3361 cttcggtata gcgcccatac ttgtcgtgtc cggaaccgcg ggcgaaccag gcgccgcgcg
     3421 agggatgcgt cccgggaagg gtgcggtacg gaatgccgtc gccgt
//
LOCUS       DASUJG010000048         2323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_503725, whole genome shotgun sequence.
ACCESSION   DASUJG010000048 DASUJG010000000
VERSION     DASUJG010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503725"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VFX38_00940"
     CDS             complement(<1..343)
                     /locus_tag="VFX38_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313462.1"
                     /translation="MSALRLELSPSRGLAAAVVSLHLAASAAVLVVFPSLAGALIAAA
                     LVALGGIAAWSRALLRSAHSVRALELDATALRIEHRDGRSFAAQLAERRYVSPRAVVL
                     VLRQPMRQAVFV"
     gene            complement(340..1578)
                     /gene="fabF"
                     /locus_tag="VFX38_00945"
     CDS             complement(340..1578)
                     /gene="fabF"
                     /locus_tag="VFX38_00945"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009913173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX5313463.1"
                     /translation="MTRRRVVVTGLGIVCPLGNAVAEAWEAAVAGKPGITRITRFDPS
                     RLACQIAGEVKGFDVSPYMSPKEARRMDLFIHYGMAAGLQAWREAGLKVTPQNAERIG
                     INFGSGIGGLPLIETMHDELNRNGPRRISPFFIPGSIINMIAGLLSIEIGAKGPNLAM
                     VTACTTSTHCIGEAAKSIRYGEADVMVAGGAEAVVSELAIGGFASARALSTRNDDPAG
                     ASRPWDKERDGFVLGEGAGAVVLEEYEHAKARGAHIYCELIGYGVSADAHHMTAPPED
                     GDGAFRAMRNALKDASLEPAAVDYINAHGTSTPPGDLAETIAIKRLLGERASRVAVNS
                     TKSMTGHLLGAAGGVEAVFTVLAIRDQVSPPTINLKTPDPACDLDYVPNRARKMPITT
                     ALSNSFGFGGTNGSLVFARL"
     gene            complement(1611..1850)
                     /gene="acpP"
                     /locus_tag="VFX38_00950"
     CDS             complement(1611..1850)
                     /gene="acpP"
                     /locus_tag="VFX38_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX5313464.1"
                     /translation="MENVEQRVKKIIAEQLGVNEAEIKNPSSFVDDLGADSLDTVELV
                     MALEEEFETEIPDEEAEKITTVQQAIDYIKAHAKA"
     gene            complement(1928..>2323)
                     /locus_tag="VFX38_00955"
     CDS             complement(1928..>2323)
                     /locus_tag="VFX38_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5313465.1"
                     /translation="VFRLSRAVMRGMMKARWGRIINITSVVGASGNAGQANYAAAKAG
                     VVGMTKSLAAELGSRNITVNCVAPGFIDTDMTRALAEEQRARLLERIPLARLGAPDDV
                     AAAVAYLASPQAGYVTGAVLHVNGGMYMS"
BASE COUNT          368 a          787 c          788 g          380 t
ORIGIN      
        1 tgacgaaaac ggcctgcctc atcggctggc gcagcacgag caccacggcc cgcggactga
       61 catagcggcg ctcggcaagc tgggcggcga agctgcgccc gtcccggtgc tcgatgcgca
      121 acgccgtggc gtcgagctcg agcgcccgca ccgagtgcgc cgagcgaagc agcgcgcgac
      181 tccaggcggc aatgcctccc agcgcgacca gcgccgcggc tatcagcgcg cccgcaagcg
      241 agggaaacac caccagaacg gccgcggaag cggcgaggtg cagcgagacg accgcggcgg
      301 cgaggccccg cgaaggcgac agttcgaggc gcagcgcgct caaagccgcg cgaaaaccag
      361 actgccgttg gtgccgccaa agccgaacga attcgaaagc gccgtcgtga tcggcatctt
      421 gcgcgcgcgg ttcggcacgt agtcgaggtc gcaggccgga tcgggcgtct tcaggttgat
      481 cgtcggcggc gagacctggt cgcgaatcgc aaggaccgtg aacaccgcct ccacgccgcc
      541 cgcggcgccc agcaggtggc cggtcatcga cttggttgaa ttcaccgcca cccgggaggc
      601 ccgctctccg agcaggcgct tgatggcgat cgtctccgcg aggtcgcccg gcggcgtcga
      661 ggtgccgtgt gcgttgatgt agtcgactgc ggcgggctcg agcgaggcat ccttcagtgc
      721 gttgcgcatg gcgcggaagg cgccgtcccc gtcctccggc ggcgcggtca tgtggtgcgc
      781 gtccgccgaa acgccgtagc cgatcagctc gcagtagatg tgcgcaccgc gcgccttggc
      841 gtgctcgtac tcttccagca ccaccgcgcc ggcgccttcg ccgaggacga agccgtcgcg
      901 ctccttgtcc cacggccggc tggcgccggc gggatcgtcg ttgcgcgtgg agagcgcgcg
      961 cgccgaagcg aagccgccga tggcgagctc cgatacgacc gcctccgcgc cgccggccac
     1021 catcacgtcc gcttcgccgt agcggatgga tttcgccgct tcgccgatgc agtgcgtgga
     1081 ggtggtgcac gcggtgacca tggcgaggtt cgggcccttc gcgccgatct cgatggaaag
     1141 caggccggcg atcatgttga tgatcgagcc tggaatgaaa aacggcgaga tgcgccgcgg
     1201 accattgcgg ttcagctcgt cgtgcatcgt ctcgatgagc ggcaggccgc cgatgcccga
     1261 gccgaagttg atgccgatgc gttcggcgtt ttgcggcgtg accttcagcc cggcctcgcg
     1321 ccaggcctgc aaccccgccg ccatgccgta gtgtatgaac aggtccatgc gccgcgcttc
     1381 cttgggcgac atgtagggcg aaacatcgaa gcccttcacc tcgcccgcga tctggcaggc
     1441 gaggcgcgaa ggatcaaagc gcgtgatgcg cgtgatgccc ggcttgcccg cgaccgcggc
     1501 ctcccacgct tcggcaaccg cgttgcccag cgggcagacg atgccgaggc cggtgacgac
     1561 gactctgcgg cgggtcactc ggtgtccact cagtttccac tcaggttcac tcaggccttg
     1621 gcgtgggcct tgatgtagtc gatggcctgc tggacggtgg tgatcttctc cgcctcctcg
     1681 tccgggatct cggtctcgaa ctcctcctcg agcgccatca cgagctccac ggtgtcaaga
     1741 gaatcggcgc cgaggtcgtc cacgaaggac gacgggttct tgatctcggc ctcgttgacg
     1801 ccgagctgct cggcgatgat cttcttgact cgttgttcta cgttttccat ccgctgctcc
     1861 ttcccttgag ttgaggggca acaccgtgct gcgagggaat cctccccccg ccaagagggg
     1921 gcgtatttta cgacatatac atgccgccgt tgacgtgcaa gaccgcgccg gtaacgtagc
     1981 ccgcctgcgg cgatgcgagg tatgcaaccg cggccgcgac atcgtcgggg gcacccaggc
     2041 gcgcaagcgg aatgcgctcg agcagcctcg cgcgctgctc ttctgcaagg gcgcgcgtca
     2101 tgtcggtgtc gatgaatccg ggcgcgacgc agttcaccgt gatattgcgg ctgcccagct
     2161 cggcggcgag cgacttggtc atgcccacca cgccggcctt cgccgcggcg tagtttgcct
     2221 ggccggcatt gcccgaggcg ccgaccaccg aagtgatgtt gatgatgcgg ccccagcgcg
     2281 ccttcatcat gccgcgcatc acggcgcggg agaggcgaaa cac
//
LOCUS       DASUJG010000049         3627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_14099, whole genome shotgun sequence.
ACCESSION   DASUJG010000049 DASUJG010000000
VERSION     DASUJG010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14099"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1789
                     /gene="parC"
                     /locus_tag="VFX38_00960"
     CDS             <1..1789
                     /gene="parC"
                     /locus_tag="VFX38_00960"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEX5313466.1"
                     /translation="GTLEEPKLLPARLPNVLLNGASGIAVGMATDIPPHNLGEVARAC
                     IHLLEAPKATVAELCEHVPGPDFPTEAELITPRPELLRMYETGAGSVRLRARHVRERS
                     GDIVVTALPYQVSGERVLAQIAEQMRAKKLPMLEDLRDESDHENPTRLVLVPRSNRVD
                     AGELMAHLFATTDLEKSYRVNLNVIGLDGRPRVKNLRELLTEWLEFRQETVTRRLQFR
                     FEKIEKRLHILEGLLVAFLHIDEVIAIIRKHDEPKPVLRKRFQLSDAQAEAILELKLR
                     HLAKLEERKIRGEQESLAKERNELRKILDSKTRLKKLIRAEITADAETYGDQRRSRMV
                     TSAPAAQALDESQLLPSEPVTVVLSEHGWVRAAKGHDMDPASLGFRTGDALRAAVRTR
                     SNHVLAFLDSTGRIYSEPVHSLPSARGYGEPLSGRSDPPPGSEFTGIAGVEDAPSALL
                     VSSSGHGFLIEAAALATRNRAGKQIVTLAPAARALTLLSVPEELGVRVALATSEGYFL
                     VLAADEIPRLARGKGVRLLAIPPKAWKAGERLVAVAVLAAGARLRIEAGQRHKFMDER
                     ELADYMRGRGKRGLKLPRGFQNVDRLLPE"
     gene            complement(1883..2893)
                     /gene="epmB"
                     /locus_tag="VFX38_00965"
     CDS             complement(1883..2893)
                     /gene="epmB"
                     /locus_tag="VFX38_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HEX5313467.1"
                     /translation="MIKASLAAVPRPDWRRELAQAIRDPAELAAILKLDPAALPAMKA
                     ASAGFRLLVPRGFAALMRRGDPDDPLLKQVLPDAAELAAQPEHYGADPLAESDAVLAP
                     GLLHKYRGRALLVTTGACAVHCRYCFRRHFPYPAENPRRKDWSAALAAIAADDSLNEI
                     ILSGGDPLMLADDELAELAASLARIAHLRRLRIHTRLPVVLPARVDEALIAWLGASAL
                     PVTMVLHINHAREIGSELAAACARLRPHLRFLLNQSVLLAGVNDDEESLAELSRRLDE
                     VGVLPYYLHLPDATAGTAHFAIGAERGRDLIAALRARLPGYLVPRLAREEPGAPAKRI
                     LA"
     gene            2950..3513
                     /gene="efp"
                     /locus_tag="VFX38_00970"
     CDS             2950..3513
                     /gene="efp"
                     /locus_tag="VFX38_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX5313468.1"
                     /translation="MASYSTNEFKSGLKIMLDGDPFSIVENEFVKPGKGQAFNRVKVR
                     NLKTGRVIERTFKSGESVEAADVLETDMQYLYSDGDFWHFMTPDTFEQYQADAAAMGD
                     AANWIKGEEICRITLYNNRPLAVEAPNTVELVITQTDPGLRGDTSSGGNKPATLETGA
                     IVRVPLFVNEGEKVRVNTRTGEYLSRA"
BASE COUNT          668 a         1157 c         1239 g          563 t
ORIGIN      
        1 cggcacgctg gaggagccga agttgcttcc cgcgcgcctt ccgaacgtgc ttctcaacgg
       61 cgcctccggc atcgccgtcg gcatggcgac ggacattcca ccccacaatc tcggcgaggt
      121 cgcgcgcgcc tgcattcacc tgctcgaagc gccgaaggcg acggtggccg aactctgcga
      181 gcacgtcccg ggaccggact ttccgaccga ggcggaactc atcacgccgc gcccggagct
      241 gttgcggatg tacgagacgg gcgcgggaag cgttcggctg cgcgcacgcc atgtccgcga
      301 acgcagcggg gacatcgtcg tcacggcctt gccgtatcag gtgtcgggcg aacgggtgct
      361 tgcgcaaatt gccgagcaga tgcgtgcgaa gaaacttccg atgctcgagg atctgcgcga
      421 cgaatccgac cacgagaacc cgacccgcct cgtactcgtt ccgcgctcca atcgggtgga
      481 tgcgggcgaa ctcatggccc atctcttcgc gaccaccgat ctcgaaaaaa gctaccgcgt
      541 gaatctcaac gtgatcggcc tcgacggccg cccgcgggtg aagaatctcc gcgaattgct
      601 gaccgaatgg ctggagttcc gtcaggaaac cgtcacgcgc cggctgcaat tccgtttcga
      661 gaagatcgag aagcggctgc acattctgga aggtttgctc gtcgcctttc tccatattga
      721 cgaagtgatt gcgatcatcc gcaagcacga cgagccgaag ccggtgctgc ggaagcgctt
      781 ccaactcagc gatgcgcagg cggaggcgat cctggagctg aaactgcgcc atctcgccaa
      841 gctcgaagag aggaaaatcc gcggcgagca ggagtcgctc gcgaaggaac ggaacgaatt
      901 gcggaaaatt ctcgattcaa aaacgcgctt gaagaagctg atccgcgcgg aaatcactgc
      961 ggatgcggag acctacggcg accagcgtcg ctcgcgcatg gtgaccagcg cgcccgcggc
     1021 gcaggccctg gacgaaagcc agttgcttcc gagcgaaccg gtgaccgtgg tgctttccga
     1081 acacggctgg gtgcgtgcgg cgaagggtca cgacatggat ccggcgagcc tcggctttcg
     1141 caccggcgac gcgctgcgcg ccgcggtgcg cacgcgcagc aaccatgtgc tcgcctttct
     1201 cgattctacc gggcgcatct acagcgagcc agtgcacagc ctgccctcgg cgcggggcta
     1261 cggcgagccg ctcagcgggc ggagcgatcc gccgcccggc agcgaattca cggggattgc
     1321 gggagtcgaa gacgcgccct cggcgctgct cgtttcatca agcggccacg gatttctgat
     1381 cgaggccgcg gcactagcga cccgcaaccg cgcgggcaag cagatcgtga cgctcgcgcc
     1441 cgctgcgcgt gcgctcacgc tcctttcggt gccggaagaa ctcggcgtgc gcgtcgcgct
     1501 cgcgacctcg gagggatatt tcctggtgct cgccgcggat gaaatcccga ggctcgcgcg
     1561 cggcaaagga gtgcgcctgc ttgcgattcc gcccaaggcc tggaaggcgg gcgagcgcct
     1621 cgtcgccgtc gcggtactgg cggcgggtgc gcggctgcgc atcgaggccg gccagcgcca
     1681 caagttcatg gacgagcgcg aactcgccga ctacatgcgc gggcgcggca agcgtggcct
     1741 caagcttccg cgcggtttcc agaacgtgga tcgcctgctc ccggaataga aactgccgga
     1801 tccgcaggcc cgcacaagac gaagcggttg cgggagattt ggatgatttc ggcgatgccg
     1861 cgatcgccaa tgccgggcag attcaggcga gaatgcgttt ggcaggggct cccggttcct
     1921 cgcgcgcgag ccgcggcacg aggtagccgg ggaggcgcgc gcgcagtgcc gcgatcaagt
     1981 ccctgccgcg ctctgcgcca atcgcgaaat gcgccgttcc ggcggtcgcg tcgggcaggt
     2041 gcaggtagta cggcaacacc ccgacctcgt cgagtctgcg ggagagttcg gcgagagatt
     2101 cttcgtcgtc gttgacgccg gcaaggagca ccgactgatt gagcagaaag cgcagatgcg
     2161 ggcgcaggcg ggcgcaggcg gccgcgagtt cggagccgat ttcgcgcgca tgattgatgt
     2221 gcagcaccat cgtcaccggc agggccgaag cacccaacca tgcgatcagg gcttcgtcca
     2281 cccgcgccgg cagcacgacg ggcaggcgcg tgtgaatgcg caggcggcgc agatgcgcaa
     2341 tgcgcgcgag gcttgcggcg agttccgcaa gttcgtcgtc cgcgagcatc agcggatcgc
     2401 cgccggaaag aatgatttcg ttgagagagt cgtccgccgc gatcgcggcc agcgcggcgc
     2461 tccagtcttt gcgtctcgga ttttcggcgg ggtaggggaa gtggcggcga aagcaatagc
     2521 ggcaatgcac ggcgcaggcg ccggtcgtga ccagcagcgc gcgtccgcgg tatttgtgca
     2581 gcaggcccgg cgcgagcacg gcatcggatt cggcgagcgg atcggctccg tagtgctccg
     2641 gctgggccgc gagttccgcc gcgtcgggaa gcacctgctt cagcagcgga tcgtcgggat
     2701 caccgcggcg catcagcgcc gcaaatccgc gcggcacgag caggcggaag cccgcggagg
     2761 cggccttcat tgcgggcagc gcggcggggt cgagcttgag aatcgcggcg agttcggcgg
     2821 ggtcgcggat ggcctgcgcc agctcgcgcc gccagtccgg gcgcggaacc gccgccaggg
     2881 aagcctttat catatgcatc tccgccgccc gcaacggcgg ccattctaag cgacagggaa
     2941 agttccgaaa tggcgagcta cagcaccaac gaattcaagt ccggactcaa gatcatgctc
     3001 gacggcgatc cgttttcgat cgtcgagaac gagttcgtca agcccggcaa gggccaggcc
     3061 ttcaatcggg tcaaggtgcg caatctgaag accggaaggg tcatcgagcg caccttcaag
     3121 tccggcgagt cggtggaggc ggccgatgtg ctggaaaccg acatgcagta tctctacagc
     3181 gacggcgact tctggcattt catgacgccc gataccttcg agcaatatca ggccgacgcg
     3241 gccgcgatgg gcgatgccgc caactggatc aagggcgagg aaatctgccg catcacgctt
     3301 tacaacaatc gcccgctcgc cgtcgaggcg ccgaacacgg tggaactcgt catcacgcag
     3361 accgatccgg gtttgcgcgg cgacacctcg agcggcggca acaaaccggc gacgctcgaa
     3421 accggtgcca tcgtgcgcgt tccgctcttc gtgaacgaag gcgaaaaggt gagggtcaat
     3481 acccggaccg gggagtacct gtcgcgcgcg tgagcgatgc ggcgccccac tggcggccgg
     3541 tggcgacgcg gcagatgctt gcacgccgcg ccgaactgat ggagcgcctg cgtgcgcatt
     3601 tccacacccg cggtctgctc gaagtcg
//
LOCUS       DASUJG010000050         5189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_138467, whole genome shotgun sequence.
ACCESSION   DASUJG010000050 DASUJG010000000
VERSION     DASUJG010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138467"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..136
                     /locus_tag="VFX38_00975"
     CDS             <1..136
                     /locus_tag="VFX38_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011257047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX5313469.1"
                     /translation="ARSPLKAGEKLADRVHAKLSSIARQPALIRSFFRHPSVAYISDF
                     "
     gene            255..407
                     /locus_tag="VFX38_00980"
     CDS             255..407
                     /locus_tag="VFX38_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313470.1"
                     /translation="MLDLQARHDWPSSFGLFPTPEIFDQKGRDLRVQADAPVHGAGAK
                     AGMKTL"
     gene            501..3761
                     /locus_tag="VFX38_00985"
     CDS             501..3761
                     /locus_tag="VFX38_00985"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HEX5313471.1"
                     /translation="MKMSNMPGPRVFYLCARLAGPIAAWGEWLARARSLGCDWLWIGE
                     LQPRAADTHPLAAIEPGGMAPELAGAGGPAKALKEFLAAARASGMRTATDFAPAFVAR
                     ASRLVRTHPHWFVREPNGEPAIPAGVGRAELAASLVACDFAGEAQTELRQFWARVTRS
                     ILQRGFDALVCRAAHRVSAASWNAILANERAGGASFWAETLGAPIEASEALAPANFDG
                     FFSSACWWDFRSGWFLAQEARLRRMAPTIAFPEEPGGRRLIADIADSERAEARARFAY
                     RYAAAVAWGVILPMGYAQGWRRPLGEAGAAEPALFDLKAEITALHKARSRSQALSAPG
                     RLELLCAPEAPVVSLASRAADPEASSPVLIALNPDCALAAATDVGPLVSRLDLTAALA
                     RELTPGRSPERLEPGKTLSLEPETVRWFELAEPDLTNAAAEITLPASFPPPVAIENVA
                     PTAGAGRFAVKRVLGETVEVSADLVAEGHARPAAALRYRLRGASEWSESPLEFFDNDR
                     WRGRFTPDRIGRWEFQILCWRDRFANWREDTIKRRDAGQSLASELGEGEAMLAEATAA
                     AGGRLKAQLVEIENLRKTNEGDADSRAGLLLAESSAEAIARALPRSEPGASAIFPVLV
                     ERGRARNGAWYECFPRSLGANGRHGNFDDLIAHLPYVAGLGFDVLYLPPIHPIGLAHR
                     KGRNNALEAAPDDPGSPWAIGGEGGGHEAIHPQLGTLGDFRRLLAAARGEGLEIALDF
                     AIQCSRDHPWIGEHPEWFRWRPDGSIRYAENPPKKYQDIVNLDFDGPGRETLWRALAD
                     VLLFWCETGVRIFRVDNPHTKPLPFWEWLFEEVRSRYPDTVFLAEAFTRPKLMYRLAK
                     LGFSQSYTYFTWRETKADLTAYLEELAGDPVVEFFRPNFWVNTPDINPFYLHDGVRAG
                     FVIRAVLAATLAPSWGMYSGYELCEHEPLCNPDGSEREEYLDSEKYELRPRDFDAPGN
                     IRAEIATLNRIRRENPAFDELTNLRFHPVTNERMMFYSKTSPDNTIWVIVSLDAHGAQ
                     EGEIELPLEIPGRGDEETVAVADLFHGGERWKWQGRRQWLRLTPERPAMILRVTRG"
     gene            3766..>5189
                     /gene="treS"
                     /locus_tag="VFX38_00990"
     CDS             3766..>5189
                     /gene="treS"
                     /locus_tag="VFX38_00990"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEX5313472.1"
                     /translation="MTEIATRADWYKDAVIYQTHVKAFFDANGDGIGDFAGLTARLDY
                     IADLGVTAIWLLPFYPSPLRDDGYDIADYRRVHPSYGTLRDFRNFVRACHARGLRVIT
                     ELVVNHTSDEHQWFQRARRAKPGSATRNFYVWSDSDQKYRGTRIIFLDTERSNWTWDQ
                     EAQAYYWHRFYSHQPDLNFDNPRVFEAIVRVLHFWMKLGVDGMRLDAVPYLVEREGTN
                     NENLPETHAVLKRIRAEVDLHFPGRMLLAEANQWPEDVSAYFGDGDECHMAFHFPLMP
                     RIYMAIAQEDRHPITDIMRQTPDIPDNCQWAIFLRNHDELTLEMVTDRERDYLWERYA
                     ADRRARINLGIRRRLAPLMEKDRRRIELMNALLLSMPGTPIVYYGDEIGMGDNVFLGD
                     RDGVRTPMQWSPDRNGGFSRADPAALYLPPIMNSEYGFEAVNVEAQQRSPSSLLNWMR
                     RLIAVRNAQSAFGRGTQRFLYPRNR"
BASE COUNT          904 a         1720 c         1655 g          910 t
ORIGIN      
        1 cgcccgcagt ccactcaagg ccggcgagaa gctcgccgat cgcgtgcacg cgaaactctc
       61 ctcgatcgct cgccaacccg ccttgattcg ttccttcttc aggcatccaa gtgttgccta
      121 tatttctgac ttctgagtaa gtcatagggt ggattgagtc cgaactgccc ggaccaccga
      181 ataagaaacc gagcgcggca gtggacagat tactcccttc ggcgtctgcg tcactacact
      241 tttgcgttac tgggatgctc gatttgcaag cgcgccacga ttggccgtca tcgtttggcc
      301 tcttcccgac gcccgagatt ttcgaccaga aaggccgaga cctgcgcgtt caggctgacg
      361 cccccgtgca cggcgcgggc gccaaggcgg gcatgaagac tctttgaact cgtgcgacga
      421 acttgccgct gcggactcgc cgagccagcc ggtccgaagc atatcgagcg gtttcgcggc
      481 ctttgggtat agtgaagggc atgaaaatgt cgaacatgcc ggggccgcgg gtcttctatc
      541 tgtgcgcgcg gctcgcggga ccgattgcgg cctggggcga atggctcgcg cgggcgcgct
      601 cgctcggctg cgactggctg tggatcggcg aactgcagcc gcgagcggcg gacacgcatc
      661 ctctggcagc gatcgagccg ggcggcatgg cgccggaact tgccggcgcc ggcggccccg
      721 ccaaggcgct gaaggagttt ctcgccgccg cgcgcgcctc gggtatgcgc acggcgaccg
      781 atttcgcgcc ggccttcgtc gcgcgtgcga gtcggctggt gcgcacgcat ccgcattggt
      841 tcgttcgaga accgaacggc gagccggcaa ttcccgcggg cgtcggccgg gcggaactcg
      901 cggcgagtct cgtcgcctgc gacttcgccg gcgaagcaca gacggaactg cggcaattct
      961 gggcgcgcgt cacgcgcagc attctccagc gcggtttcga tgcgctcgtc tgccgcgccg
     1021 cgcaccgcgt ttcggccgca agctggaacg cgattctcgc gaacgaacgt gccgggggcg
     1081 cgagcttctg ggccgagaca ctcggcgccc cgatcgaggc gagcgaggcg ctcgcgccag
     1141 ccaatttcga cggcttcttt tcgagcgcct gctggtggga ctttcgctcc ggctggttcc
     1201 tcgcgcagga ggcgaggctg cgccgcatgg cgccaaccat cgccttcccg gaagaacccg
     1261 gaggaaggcg cctcattgcc gatatcgccg attcggagcg cgcagaagcg cgggcgaggt
     1321 tcgcgtatcg ttatgcggcg gcagttgcct ggggcgtgat tttgccgatg ggctatgcgc
     1381 agggttggcg tcgcccgctc ggcgaagccg gagcggcgga gccggcactc ttcgatctga
     1441 aggcggaaat cacggcactg cacaaagcgc gttcccgctc gcaggctttg tccgcaccgg
     1501 gtcggctcga gcttctctgc gcccccgagg cgcccgtcgt aagcctcgcc tcacgcgcgg
     1561 ccgatcccga agcatcgtcg ccggtgttga tcgcgctcaa tcccgactgc gcgcttgccg
     1621 cggcaactga cgtcggcccg ctcgtctcgc gactcgacct gaccgcagct ctcgcccggg
     1681 agctgactcc cggaagaagt cccgaacgcc tcgagccggg aaagacgctg agtctcgaac
     1741 cggaaaccgt gcgctggttc gaactcgcgg agccggatct cacgaatgcg gcggcggaaa
     1801 tcacgctccc ggcgtccttc cccccgccgg tcgcgatcga aaacgttgct ccgaccgcgg
     1861 gcgccgggcg cttcgcggtg aagcgcgtgc tcggggaaac cgtcgaagtt tccgccgatc
     1921 tcgtcgccga agggcacgcc cggccggcgg ccgcgctgcg ttatcggctt cggggcgcga
     1981 gcgaatggag cgaatcgccg ctggaattct tcgacaacga tcgctggcgc ggaagattca
     2041 caccggatcg catcggccgc tgggagtttc agatcctctg ctggcgcgat cgtttcgcca
     2101 actggcgcga ggacacgatc aagcgccgcg acgccgggca gtcgctcgcc tccgaactcg
     2161 gcgaaggcga agcgatgctc gcggaggcga cggccgccgc gggtgggcgg ctcaaggcgc
     2221 aactcgtcga aatcgaaaac ctgcgcaaga cgaacgaagg cgatgccgat tcgcgggccg
     2281 gattgttgct ggccgagagc agcgccgagg caatcgcgcg cgccttgccg cggagcgagc
     2341 cgggagcctc cgcgattttt cccgtgctcg tggagcgcgg ccgggcacgc aacggcgcct
     2401 ggtacgaatg cttcccgcgg tcgctcggcg cgaacggccg tcacggcaac ttcgacgatc
     2461 tcatcgcgca tcttccctac gtcgcgggtc tcggtttcga cgtgttgtat ctgccgccca
     2521 ttcatccgat cggactcgcg caccgcaagg ggagaaacaa cgcgctggag gccgcacccg
     2581 acgatcccgg cagtccctgg gcgatcggcg gcgaaggggg agggcacgag gcgattcatc
     2641 cgcaactcgg aacgctcggc gacttccgcc gcctgctcgc ggcggctcgc ggcgagggac
     2701 tcgagatcgc gctcgacttc gcgattcagt gctcgcgcga tcatccctgg atcggcgagc
     2761 atcccgagtg gttccgctgg cggccggacg gaagcatccg ctacgcggaa aatccaccga
     2821 agaaatatca ggacatcgtc aatctggatt tcgacggccc cgggcgggag acactgtggc
     2881 gcgcgctcgc cgacgtgctt ctgttctggt gcgagacggg cgtgcgcatc ttccgggtgg
     2941 acaatccgca caccaagccg ctgccgttct gggagtggct cttcgaggag gtgcgcagcc
     3001 gttacccaga tacggttttt ctcgccgagg cgttcacccg cccgaagctc atgtatcggc
     3061 tcgcgaagct cggattctcc cagtcctaca cctacttcac ctggcgggag acgaaggcgg
     3121 acctcacggc ctatctcgaa gaactcgcgg gcgatccggt cgtcgaattt ttccgtccga
     3181 atttctgggt caacaccccc gacatcaatc ccttttacct ccacgacggc gtgcgcgccg
     3241 gcttcgtgat ccgagccgtg cttgcggcga cgctcgcgcc gagctggggc atgtactcgg
     3301 gctacgagct gtgcgagcac gaacccttgt gcaatccgga cggaagcgag cgcgaggaat
     3361 atctcgattc cgaaaaatac gagttgcgcc cgcgcgattt cgatgcaccc ggtaacatcc
     3421 gcgcggaaat cgcgacgctc aatcgcatcc gccgcgaaaa cccggccttc gacgagttga
     3481 cgaatctgcg ctttcacccg gtgacgaacg aacgaatgat gttctactcg aagacatcgc
     3541 cggacaatac gatctgggtg atcgtgagtc tcgatgcgca cggcgcgcag gagggcgaga
     3601 tcgagctgcc gctcgaaata cccggaagag gtgacgagga aacggttgcc gttgccgatc
     3661 tctttcacgg cggcgaacgc tggaaatggc agggccggcg ccaatggctg cgcctgacgc
     3721 cggaacgccc ggcgatgatc ctgcgagtaa cgcgaggttg acttcatgac ggaaatcgcg
     3781 acgagggcgg actggtacaa ggacgccgtc atctatcaga cgcacgtcaa ggcgttcttc
     3841 gacgccaacg gcgacggcat cggcgacttc gcgggtttga ccgcacgcct cgactacatc
     3901 gcggacctcg gcgtcacggc gatctggctt ctgccgtttt acccgtcgcc gcttcgcgac
     3961 gacggctacg acatcgccga ctaccgtcgc gttcatccga gttacgggac gctgcgcgac
     4021 tttcgcaact tcgtgcgggc ctgccacgcc cgcggacttc gggtcatcac cgaactcgta
     4081 gtcaatcaca cctccgacga gcaccagtgg tttcagcgcg cccgccgcgc gaagccgggc
     4141 tccgccacgc gcaatttcta cgtctggagc gacagcgacc agaaataccg gggcacccgc
     4201 atcatttttc tcgacacgga acgttccaac tggacatggg atcaggaggc gcaggcctac
     4261 tactggcatc gcttctattc gcatcagccg gatctcaact tcgacaatcc gcgcgtcttc
     4321 gaggcgatcg tccgcgtgct ccacttctgg atgaaactcg gcgtggacgg catgcgtctc
     4381 gatgccgttc cctatctcgt cgagcgcgaa gggaccaaca acgagaatct ccccgagacg
     4441 cacgccgtgc tcaaacgcat tcgcgccgag gtcgatctcc attttcccgg gcgtatgctg
     4501 ctcgccgagg ccaatcagtg gccggaagac gtgagcgcct acttcggcga tggcgacgaa
     4561 tgtcacatgg ccttccactt cccgctgatg ccgcgcattt acatggcgat cgcccaggaa
     4621 gaccgccatc cgatcaccga catcatgcgc cagacgcccg acattcccga caactgccaa
     4681 tgggcgatat tcctgcgcaa tcacgacgaa ctcacgctcg agatggtgac cgatcgcgag
     4741 cgcgattatc tgtgggaacg ctacgcggcg gaccggcgcg cgcgcatcaa tctcggaatc
     4801 cgccgccggc tcgcgccgct catggaaaag gatcgccggc gcatcgagct gatgaatgcg
     4861 ctgctcctgt cgatgccggg cactccgatc gtctactacg gcgacgagat cggcatgggc
     4921 gacaacgtgt ttctgggtga ccgcgacgga gtgcgcaccc cgatgcagtg gtcgccggat
     4981 cgcaacggag gcttctcgcg cgccgatccc gcggctcttt atctgccgcc gatcatgaat
     5041 tccgagtacg gcttcgaggc cgtcaacgtc gaggcccagc aacgcagccc ctcgagtctg
     5101 ctcaactgga tgcgccgcct gatcgccgtg cgcaacgcgc agagcgcctt cggacgcggc
     5161 acgcagcgtt ttctctatcc gcgaaatcg
//
LOCUS       DASUJG010000051         2011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_994448, whole genome shotgun sequence.
ACCESSION   DASUJG010000051 DASUJG010000000
VERSION     DASUJG010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994448"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VFX38_00995"
     CDS             complement(<1..438)
                     /locus_tag="VFX38_00995"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX5313473.1"
                     /translation="MSTASAIRPHALPRRLGLASGMAVVVGIIIGSGIFRVPSPIAAE
                     AGNLTGIALVWILGGVVSLFGALSIAELAAMFPQAGGPYVYLREAWGRPLAFLFGWMW
                     LLTSPISKAAQSLIFAEYLAFFVPLSNTAVHLIAAALILVVAAA"
     gene            863..1804
                     /locus_tag="VFX38_01000"
     CDS             863..1804
                     /locus_tag="VFX38_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5313474.1"
                     /translation="MRMRRLGRTGLEVSEVGLGCNNFGARVDLETSRAVVHAALDAGV
                     NFFDTADVYGGRGGSESILGRVLGERRKDVVLATKFGSAMEEGVERRNASRRYILQAV
                     EASLKRLKTDWIDLYYVHWPDKLTPLDETLRALDDLVRSGKVHYVGCCNFAAWRVVAA
                     EWIARELKLHRFVCAQNEFSLLERAPEKELLPALAEYDLGLVPYFPLASGLLTGKYER
                     DKPPPDDSRLAQWKRLAESYATPTNWDLIEKLEAWAGERDHSLLEFAFAWLLAHGAVA
                     SVIAGATKPEQVAANVRAAEWRLDADDLASLREILDG"
BASE COUNT          343 a          628 c          708 g          332 t
ORIGIN      
        1 cgccgcggcg acgacgagga tgagtgccgc ggcgatcaga tgcaccgccg tgttggaaag
       61 cggcacgaaa aacgcaaggt attcggcgaa gatcaggctt tgcgcggcct tggaaatcgg
      121 cgaggtgagc agccacatcc agccgaagag aaacgcgagc ggccggcccc aggcttcgcg
      181 cagatacacg tatggaccgc ccgcctgcgg aaacattgcc gcgagttcgg cgatcgacaa
      241 cgcgccgaag agcgacacca cgccgcccag aatccagacg agcgcgatgc cggtgagatt
      301 gccggcctcg gcggcgatcg gcgagggcac gcggaagata ccgctgccga tgatgatgcc
      361 gacgacgacc gccatgccgg aggcgagccc gagccggcgc ggaagtgcgt gcggccggat
      421 ggcggaagcg gtgctcatgg ccgcgcagcg taccatcccg cggcgatcgg ctcaggaagc
      481 cgcttgggat cggcttcgtg ccgcgcgcaa cttcttttgg gttctttccg cccccttcgg
      541 aacgaacggg gccgcggttt cgcgggggga tgtctttgcg gcccgcggat tggcgccagt
      601 cttcgcaaag acataccgga ggaagacatc cccctcgatc ccccttcctg tggaaggggg
      661 aagttgtcgt cgccgccttt ctgcggcagg gtggctgctc gcggcttctt ccgggttctt
      721 tccgccctct tcggaacgaa gggggccgcg tcttcgcggg gggatgtctt tgcggcccgc
      781 ggattgacgc cagtcttcgc aaaggtcggg gcgcccgcgg gcgcggtacc atgaaaatgc
      841 acttggcata aaggagcgga acgtgaggat gcgcagactc gggcggacgg gcctcgaagt
      901 ttcggaagtc gggctcggct gcaacaattt cggcgcccgc gtggatctcg agacctcgcg
      961 cgccgtcgtg catgccgcgc tcgatgcggg cgtcaatttc ttcgacaccg ccgatgtgta
     1021 cggcgggcgt ggcggctcgg aaagcattct cggccgcgtt ctcggcgagc ggcggaagga
     1081 cgtggtgctc gcgaccaagt tcggctcggc gatggaagag ggcgtcgagc ggcgcaacgc
     1141 ctcgcgccgc tacatcctgc aggcggtcga ggcgagtctc aagcggctca agaccgactg
     1201 gatcgatctc tattacgtgc actggccgga caaactcacg ccgcttgacg agacgctccg
     1261 cgcgctcgac gatctggtgc gctcgggcaa ggtgcattac gtcggctgct gcaacttcgc
     1321 cgcctggcgg gtggtcgcgg cggaatggat tgcgcgcgag ctgaaactgc atcgcttcgt
     1381 ctgcgcgcag aacgagttca gtctgctgga gcgcgcgccg gagaaggaac tgctgccggc
     1441 gctcgccgag tacgatctcg gcctcgtgcc gtattttccg ctcgcgagcg ggcttttgac
     1501 cggcaagtac gagcgcgaca agccgccgcc ggacgattcg cggctcgcgc agtggaagag
     1561 gctcgccgaa tcctatgcca cgccgacgaa ctgggatctc atcgagaagc tcgaagcctg
     1621 ggcgggcgag cgcgatcact cgctgctcga gttcgccttc gcatggctgc tcgcccacgg
     1681 cgctgtcgcg agcgtgatcg ccggcgcgac caagccggag caggtcgcgg ccaacgtgcg
     1741 cgcggccgag tggcgtctcg atgccgacga tctcgcgagc cttcgcgaaa ttctcgacgg
     1801 ctagaggata ggtagcgcag gatgcggtga ccttgcgaat cacatcgtcc gactccgctt
     1861 tgtagatgcg gttcgaggac tcaccacatc ctgctcggag gagacgttcg gcgaggccga
     1921 gaggcacgac ggatgcgccc gagcgatgcc cgcgcggcgg atcggattta tcatgatgcg
     1981 gttcgcgacc tgccggaacc ggcgccgcgc c
//
LOCUS       DASUJG010000052         3493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_749235, whole genome shotgun sequence.
ACCESSION   DASUJG010000052 DASUJG010000000
VERSION     DASUJG010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749235"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /gene="panB"
                     /locus_tag="VFX38_01005"
     CDS             complement(<1..718)
                     /gene="panB"
                     /locus_tag="VFX38_01005"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006914297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX5313475.1"
                     /translation="MSYADSDGEYRPRTLATFAKMREAGEPIACLTAYDASFARLLDE
                     AGVDIVLVGDTLGMVVQGRTSTVPVTMDDMVYHTRLVARGLERAYLVGDMPFLSYVDP
                     PTAVRNAGRLLQEGGARMVKLEGTGAQAEVVAALAANGIPVCAHLGLRPQAVEKLGGF
                     HVQGRAREDAERMLADARELEAAGADIILLECIPRALAARIHEAASVPVIGIGAGPDV
                     DGQILVLYDILDITSGHKPRF"
     gene            complement(715..1362)
                     /locus_tag="VFX38_01010"
     CDS             complement(715..1362)
                     /locus_tag="VFX38_01010"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEX5313476.1"
                     /translation="MNRAAPGYVAIEGPIGVGKTSLARRLAASFEAEAVFEEAEENPF
                     LERFYRDQREAALPTQLSFLFQRTRKLEALAQSDLFHPAWIVDFVMEKDLLFAELTLD
                     DAELALYHEVYRRVARELPAPELVIYLQAPVEVLLKRIAARGRSFERTVPRAYLERVV
                     EAYTHFFHHYNRSRLLIVNAATADPVHEDADYERLLAEIRGGRGDRSYFNPEAGR"
     gene            complement(1359..1844)
                     /gene="folK"
                     /locus_tag="VFX38_01015"
     CDS             complement(1359..1844)
                     /gene="folK"
                     /locus_tag="VFX38_01015"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX5313477.1"
                     /translation="MTAAWIGLGSNLGDSRAEIEAAFAELDSLPHTSLRSRSRLYRTR
                     PVGPVAQPDFVNAAAALETALAPEELLDALLAVEARHGRERREHWGPRTLDLDILLYD
                     ETQIATARLSVPHPRLAERAFALCPLADIAPQAEVPGAGSVAVLLARLDTSGVTPLAP
                     A"
     gene            complement(1841..3088)
                     /gene="pcnB"
                     /locus_tag="VFX38_01020"
     CDS             complement(1841..3088)
                     /gene="pcnB"
                     /locus_tag="VFX38_01020"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEX5313478.1"
                     /translation="MATPAAVPEGDVGFAAQRIDSRALDIVRTLIEAGYEAYVVGGGV
                     RDILLDERPKDFDIATAARPEEIRKLFRRARVIGRRFPIVHVFWGRDYVEVTTFRALA
                     GEDRQIHASGRILRDSTYGTLAEDALRRDFTINALYYDPRSRQVLDFTGGLADIEGRL
                     IRLIGDARQRYREDPVRMLRALRFAAKLDFSLEAETEAPIREMAPLLGDIAAARLFEE
                     FGKLFLTGHALATFRLLRRYSLFGELFPLAEEVLAADGAGPFPRFVESALASTDERAA
                     AGESVTHAFLLAALLWPPVARYAAWLAREGMGEATAVQRATSVVLGEAAKRVTVPRRI
                     AQPLREILQLQTRFRYRHGKRAESLFAHPRFRAAWEFLRLRAAAGDGDPALAEFWTRA
                     QASDAEGRRKLFSPAGTRHRKAK"
     gene            3139..3214
                     /locus_tag="VFX38_01025"
     tRNA            3139..3214
                     /locus_tag="VFX38_01025"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3173..3175,aa:Glu,seq:ctc)
     gene            3243..>3493
                     /locus_tag="VFX38_01030"
     CDS             3243..>3493
                     /locus_tag="VFX38_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HEX5313479.1"
                     /translation="MGRRILLLVSLLFAGVPLASLAAAPAPAAGSAPSVLAYLKQQGL
                     KINSRFTAMGGLTGYAGTTPDGSPVIFYVPADGSVAIFG"
BASE COUNT          608 a         1180 c         1160 g          545 t
ORIGIN      
        1 cgaagcgcgg cttgtggccg ctggtgatgt cgagaatgtc gtagagcacg agaatctgtc
       61 cgtccacgtc gggccccgcg ccgatgccga ttacgggtac cgaagccgct tcgtggatgc
      121 gtgccgcgag cgcgcgcgga atgcattcga gcaggatgat gtcggcgcct gcggcctcga
      181 gttcgcgggc atcggcgagc atgcgctcgg catcctcgcg cgcgcgtccc tgcacgtgga
      241 aaccgccgag cttttccacc gcctgcgggc gcagccccag atgcgcacag accggaatgc
      301 cgttcgccgc gagcgccgcg acgacttcgg cctgcgcgcc cgtgccttcg agtttgacca
      361 tgcgcgcacc gccctcctgc agaaggcgtc cggcattgcg caccgccgtc ggcggatcga
      421 cgtaactcag aaagggcatg tcgccgacga gataggcgcg ctcgaggccg cgggcgacga
      481 ggcgcgtgtg atagaccatg tcgtccatcg tcaccggcac cgtgctcgtc cgtccctgca
      541 ccaccatccc gagggtgtcg ccgacgagca cgatatccac accggcctcg tcgagcagcc
      601 gcgcgaaact cgcgtcgtag gcggtgaggc aggcgatggg ctcgcccgct tcccgcattt
      661 tcgcaaaggt cgcgagcgtc cgcgggcggt attcgccgtc gctgtccgcg taactcatcg
      721 ccccgcctcc ggattgaaat agctccgatc cccgcgcccg ccgcggattt cggcgagcag
      781 acgttcgtaa tccgcgtcct catgtaccgg atccgccgtc gccgcattca cgatcagcaa
      841 acgcgaacgg ttgtaatgat gaaagaaatg ggtataggcc tcgacgacgc gctcgaggta
      901 cgcccgcgga accgtgcgct cgaagcttcg cccgcgcgcg gcgatccgct tgagaagcac
      961 ctccactggc gcctgcaggt agatcacgag ttcgggagcc ggcaactccc gcgcgacgcg
     1021 acggtatacc tcgtgataga gcgcgagttc ggcatcgtcg agcgtaagtt ccgcgaaaag
     1081 aagatccttt tccatcacaa agtccacgat ccacgccgga tggaaaagat cggattgcgc
     1141 gagcgcttcg agctttctcg tgcgctgaaa aagaaaggac aattgcgtcg ggagcgcggc
     1201 ctcgcgctga tcgcggtaga aacgttcgag aaagggattc tcctccgctt cctcgaacac
     1261 cgcctccgcc tcgaagctcg ccgcgaggcg gcgggctaga ctcgtcttgc cgacgccgat
     1321 cggcccctcg atcgccacgt agcccggcgc cgcgcgattc atgcgggggc gagcggcgtg
     1381 acgccgctcg tatcgagacg tgcgaggaga accgcaacgc tcccggcgcc cgggacttcg
     1441 gcctgcggcg cgatgtcggc aagcgggcaa agcgcaaagg cgcgctcggc gaggcgcgga
     1501 tgcggcacgc tcagacgcgc cgtcgcgatc tgcgtctcgt cgtacagaag aatgtcgaga
     1561 tccagcgtcc gcggccccca gtgttcgcgc cgctcccgcc cgtgccgcgc ctcgacggcg
     1621 agcaaggcgt cgagcagctc ctcgggtgcg agcgcagttt cgagcgcggc cgcggcattg
     1681 acgaaatcgg gctgcgcgac ggggccgacg ggacgcgtgc ggtacagccg cgagcgggag
     1741 cggaggctcg tgtgcggaag cgaatcgagt tcggcgaacg ccgcctcgat ctcggcgcgc
     1801 gaatcgccga gattgcttcc gagtccgatc caggccgccg tcatttcgcc ttccgatggc
     1861 gggtgccggc cggggagaaa agcttgcgcc gtccctcggc atcactcgcc tgagcgcggg
     1921 tccagaattc ggcgagcgcc ggatcgccgt cgccggccgc cgcgcgcaga cgcagaaatt
     1981 cccaggcggc gcgaaagcgc ggatgtgcga agagactctc cgcacgcttg ccgtggcggt
     2041 agcgaaagcg cgtctgcaat tgcagaattt cacgcaaggg ctgcgcgatg cgccgcggca
     2101 ccgtcacgcg cttcgcagcc tcgccgagca cgaccgaggt cgcgcgctgc accgccgtcg
     2161 cctcgcccat gccctcccgc gcgagccagg cggcgtagcg cgcaaccggc ggccacagca
     2221 gggcggcgag gagaaacgca tgggtgacgg attcgccggc ggcggcacgc tcgtcggtcg
     2281 aggcgagcgc cgattcgacg aaacgcggaa aaggccccgc accatcggcg gcaagaactt
     2341 cctcggccag ggggaaaagc tcgccgaaaa gactgtagcg gcgaagcaga cgaaacgtgg
     2401 cgagcgcgtg gcccgtcaga aacagtttgc cgaattcctc gaacaggcgg gccgccgcga
     2461 tgtcgccgag aagcggcgcc atttcgcgga tcggcgcctc ggtttcggcc tcgagcgaga
     2521 agtcgagctt ggccgcgaat cgcagcgcgc gcagcatccg caccggatcc tcgcggtagc
     2581 gttggcgggc atctccgatc aggcggatca ggcggccctc gatatccgcg agcccacccg
     2641 tgaaatcgag cacctgacgg ctgcgcggat cgtagtagag cgcgttgatc gtgaagtcgc
     2701 gacgcagcgc atcctcggca agcgttccgt aggtggagtc gcgaagaatt ctccccgagg
     2761 cgtggatctg gcgatcttcg ccggcgagcg cgcgaaacgt cgtcacctcg acgtaatcgc
     2821 ggccccagaa gacatgcacg atcggaaagc gccgcccgat gacgcgagca cggcgaaaca
     2881 gtttgcgaat ttcctcgggg cgggcggccg tcgcgatatc gaagtctttc ggccgctcgt
     2941 cgagcaggat gtcccgcacg cccccgccca ccacataggc ttcgtaaccg gcctcgatca
     3001 gggtacggac gatgtcgagc gcgcgcgaat cgatgcgctg cgcggcaaag ccgacatccc
     3061 cttcgggcac ggccgcaggc gtcgccaaat cgttccttcc cttgcgtgta accgaaaatg
     3121 gtaccatttt gatcctttgc tcccatcgtc tagcggccca ggacgtcgcc ctctcacggc
     3181 gaaaaccggg gttcgagtcc ccgtgggagc gccacatcgg ctgttgccgc ggagtttcaa
     3241 ggatgggccg cagaatcctg ctactggttt cactgctgtt tgcgggggtg ccgctcgcaa
     3301 gtctcgcggc cgcgccggcg ccggcggcgg gctccgcccc ttcggtactc gcctacctga
     3361 aacagcaggg gctcaaaatc aactcgcgtt tcaccgccat gggcgggctc accggctacg
     3421 ccggaaccac cccggacggc agtcccgtca tcttttacgt gcccgccgac ggctcggtcg
     3481 ccattttcgg aca
//
LOCUS       DASUJG010000053         3136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_749374, whole genome shotgun sequence.
ACCESSION   DASUJG010000053 DASUJG010000000
VERSION     DASUJG010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749374"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /gene="rmuC"
                     /locus_tag="VFX38_01035"
     CDS             complement(<1..574)
                     /gene="rmuC"
                     /locus_tag="VFX38_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEX5313480.1"
                     /translation="MSLVEILLIVLLAAVALVLLAQATILFRARGDGGLARRLDVLKN
                     DGERIERALREEQRAGRGELSQSFEKFALLTQRQLDSMGTQQRERIEDFAHRLSQMSG
                     DNEKRLGEIRGTLEAQLKALREENTKQLERMRATVDEKLQTTLETRLGQSFKLVSERL
                     EAVQRGLGEMQTLATGVGDLKRVLSNVKTRG"
     gene            complement(627..1493)
                     /locus_tag="VFX38_01040"
     CDS             complement(627..1493)
                     /locus_tag="VFX38_01040"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX5313481.1"
                     /translation="MHDLLGAIVLGIVEGITEFLPISSTGHLLIVEHWLGARSDLFNV
                     GIQAGAILAITLVYRQRIWQLLTNWGERANRVYLYKLILAFLITVVIGFVAVEAGFQL
                     PTTVTPVAWALIIGGLWMLFAEWAAERIERRRKAAGRVAVLEDGEVRYATAGYSSTHV
                     TWKVAILVGVAQMIAGLFPGTSRSAATIFTAMLFGTSNRSAATEFAFLVGIPTMYAAT
                     AYEAYATLKDGNAAHASWGALGVGFVVSLIVAFAAVKWLLGYIRNHRFTPFAIYRIAL
                     GIALLLIIPMGH"
     gene            1672..2607
                     /locus_tag="VFX38_01045"
     CDS             1672..2607
                     /locus_tag="VFX38_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014741160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEX5313482.1"
                     /translation="MTQAQTCYPSGKQWTIRGAGKTADAFATIVEIGGGLRQYESGGA
                     PVLESFALDAICDGAHNMPLVPWPNRIRDGRYSFDGHEYQLGLTEPALHNAIHGFGRW
                     RNWTLRAREPDRLVVGLLLHPQPGYPFTLDVAIEYVLATEGLTITTTATNRGARACPW
                     GFGQHPYFAAGEGAIDRLTLEFDAAERLTIDARQIPTGRVPVAGSIHDFRGGRTIGNL
                     KLDDAFTALARDEEGKIRAWLGSPGGPRIGLWADEHFRYLQLYSGDTLAPERRRRALA
                     IEPMTCAPNAFASGDGLMRLEPGETFHCSWGIVTD"
     gene            complement(2993..>3136)
                     /locus_tag="VFX38_01050"
     CDS             complement(2993..>3136)
                     /locus_tag="VFX38_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015006228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313483.1"
                     /translation="EAVLAMEYRASAEDIQRTIHAHPTLSEAMHEAALAVDKRAIHVV
                     NRR"
BASE COUNT          573 a         1001 c         1010 g          552 t
ORIGIN      
        1 tgccgcgggt tttgacgttg ctgagtacgc gcttgagatc gccgactccc gtggccagcg
       61 tctgcatctc gccgagtccg cgctgcaccg cttcgaggcg ctcggagacc agcttgaagg
      121 actgcccgag acgggtttcc agcgtcgtct ggagtttttc gtccacggtc gcgcgcatgc
      181 gctcgagctg cttcgtgttt tcttcacgca gcgccttcaa ctgggcttcg agcgtgccgc
      241 ggatttcgcc gagccgtttt tcattgtcgc cggacatttg cgagagccgg tgcgcgaagt
      301 cttcgatgcg ttcgcgctgc tgtgtaccca tcgagtcgag ctgccgctga gtgagtaatg
      361 cgaacttctc gaaggattgg gagagttcgc cgcgcccggc gcgctgctct tcgcgaaggg
      421 ctcgctcgat gcgctcgccg tcgttcttga gtacgtcgag acggcgcgcg agcccgccat
      481 cgccgcgcgc gcgaaagagg atcgtcgcct gcgcgagaag cacgagcgcg accgcggcaa
      541 ggagcacgat caggaggatt tccaccaaag acacggaggt tccctaccct aaacccggtc
      601 agtatactgc gcggcgttcg ctccgctcag tggcccatcg ggatgatgag gagcagcgcg
      661 atgccgagcg cgatgcggta aatcgcgaag ggcgtgaagc gatgattgcg gatgtagcca
      721 agcagccatt tcaccgcggc gaaggcgacg atcagcgaga ctacgaagcc gacgccgagc
      781 gcgccccagc tcgcatgcgc ggcgttgccg tccttcaacg tggcatacgc ttcgtaggcg
      841 gtcgccgcgt acatggtcgg gattcccacc agaaacgcga attcggtcgc cgcggaacga
      901 ttgctggtgc caaacagcat cgcggtgaaa atggtcgcgg ccgagcgcga ggtaccggga
      961 aagagtccgg cgatcatctg cgcgacgccg accagaatcg cgaccttcca ggtgacgtgg
     1021 gtgctgctgt agccggctgt ggcatatcgc acttccccgt cctcgagcac ggcgacccgg
     1081 cccgcagcct tgcgccgccg ctcgatccgt tccgcggccc actcggcaaa gagcatccac
     1141 agcccgccga tgatgagcgc ccaggcgacc ggcgtcacgg tggtcggcaa ttggaaaccg
     1201 gcctccaccg cgacgaatcc gatcacgacg gtgatcagga aggcgaggat caacttgtac
     1261 agatagacgc gattcgcgcg ctcgccccaa ttggtgagga gttgccagat gcgttggcgg
     1321 tacacgagcg tgatggcgag gatggcgccg gcctgaatgc cgacgttgaa caggtccgaa
     1381 cgagcgccca gccaatgctc gacgatcaga agatgaccgg tgctcgagat gggcagaaac
     1441 tcggtgatcc cctccacgat gccaaggacg atggcgccga gaagatcgtg catggacgaa
     1501 cccgcccaaa agatcgccta gtgtactccg gatcgcccgg gggaagttgc cgaaacgacc
     1561 tgcaaaagtt cttttccgcg cacgccgcca tccgctcccg cccggccgga aacgggcggg
     1621 aacttccccg atgccgaccg gtcaattgcg actgcgttat catccgcaga tatgacgcag
     1681 gcgcagactt gctacccctc gggcaagcaa tggacgatcc ggggcgcggg caaaacggca
     1741 gatgcattcg cgacgatcgt cgagatcggc ggtgggctgc ggcaatacga atccggcggc
     1801 gcgcccgtgc tcgagtcctt cgcgctcgat gcgatctgcg acggcgcgca caacatgccg
     1861 ctcgttccct ggccgaatcg catccgcgac ggccgctatt ccttcgacgg ccacgagtat
     1921 caactcggcc tgaccgaacc cgcattgcac aacgcgattc acggtttcgg gcggtggcgc
     1981 aactggacgc ttcgcgcgcg ggagccggat cgccttgtcg tcggactgtt gcttcatccg
     2041 caaccgggct atccgttcac gctcgacgtc gcgatcgaat atgtgctcgc aacggaggga
     2101 cttacgatca cgaccaccgc gaccaatcgc ggtgcacgcg cctgcccctg gggcttcggc
     2161 caacatccct actttgccgc cggcgagggc gcgattgacc ggttgacgct ggaattcgac
     2221 gccgcggagc gattgacgat cgacgcccgg cagattccga ccggacgcgt tcccgtcgcc
     2281 ggatcgatcc atgactttcg cggcggaagg acgatcggaa acctgaaact ggacgacgcc
     2341 ttcaccgcac tcgcgcgcga tgaggaagga aagatccgcg cgtggctggg ttcgcccggc
     2401 ggccctcgca tcgggctctg ggcggacgag cattttcgtt atcttcagct ctacagcggc
     2461 gatacgctcg cgcccgaacg gcggcgacgc gccctcgcga tcgagccgat gacctgtgct
     2521 ccgaatgcat tcgcaagcgg cgatggcctg atgcggctcg aaccgggcga aacgttccat
     2581 tgctcctggg gaatcgtcac ggactgagca ttctcccgga cgcgaacgtc gcatgccgac
     2641 aacgccttcg gtctcgttcc ggcctgcggg actaccgacg tattgtattg atgcaccaca
     2701 tcccgagccc gacagccccg ggataagcgt gtttacgcgc atcgtggcaa tcggcgttca
     2761 atgccggcaa cggtcttgcg accgtatgcc ggcctacggg actcatttac ggttgcggga
     2821 tgcgaggggc tatggtggca gaaggcgtac ggaaagccga tcgggataga gaggttgcaa
     2881 ggccgcgacc ccaataaccg gctcgcgttc cgtgcgcagt tgccggcaac gcccttcggg
     2941 cttataccgg cctacggaag cgatgcttgc aacgaacgag accccgattg gatcagcggc
     3001 ggttgacgac gtggatcgcg cgcttgtcca ccgcgagcgc cgcctcgtgc atcgcctcgg
     3061 acagcgtcgg atgcgcgtgg atcgtgcgct ggatgtcttc ggcgcttgcg cggtattcca
     3121 tcgcgagcac ggcctc
//
LOCUS       DASUJG010000054         2257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1238702, whole genome shotgun sequence.
ACCESSION   DASUJG010000054 DASUJG010000000
VERSION     DASUJG010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238702"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VFX38_01055"
     CDS             complement(<1..586)
                     /locus_tag="VFX38_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEX5313484.1"
                     /translation="MSGTIRIVLVATSHPGNVGAAARAMKVMGLGSLVLVAPQCEIGE
                     EARARARGALDVLEKASCVDTLDEAIVGAGFVVATSARPRHLGPAVVAARDGAAELVA
                     ELAARDVAILFGPERSGLDNDALDRANRLWYIPTAGDRGSLNLAAAVQVIAYEVLLAI
                     NAALPRAPPPAAALAEDVAALCAHFDGVARSLGVI"
     gene            655..1536
                     /locus_tag="VFX38_01060"
     CDS             655..1536
                     /locus_tag="VFX38_01060"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX5313485.1"
                     /translation="MDPLANIAVSAARRGGAVILRSLDRLDLLKVETKRHNDFVTEID
                     RRSEATIIETIRRAHPDHAILAEERGREDTGSAIEWIIDPLDGTTNYLHGFPAFSVSI
                     AVSERGRLVTGVVYDPLREEMFVASRGQGALLNERRIRVAARPALEGALVGTGFPFRE
                     LRDLDDYLAMFRAVIERTSGLRRAGSAALDLAWTAAGRLDGFFELGLEVWDIAAGCLL
                     IEEAGGIIGDVCGNAGWPIAEGNILAGNPRIFAALARALAPHLPPRLASQPAAERSDS
                     PRAKTARADPLRKSKRK"
     gene            complement(1679..>2257)
                     /gene="secF"
                     /locus_tag="VFX38_01065"
     CDS             complement(1679..>2257)
                     /gene="secF"
                     /locus_tag="VFX38_01065"
                     /inference="COORDINATES: protein motif:HMM:NF014416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX5313486.1"
                     /translation="RQKQLNTVGPQVGSQLRTQGAMALVVALILIFIYLLVRYEWRLA
                     LGAIFATLHDVVFIIGIFSFTHLKFDLTVLAAVLAMLGYSVNDTVVIFDRIREDFKRV
                     RRGSSYEVINLAVNQTLSRTIMTHVMTQLVVIALLVVGGPPLREFSIALSIGIVVGTY
                     SSIYVASASAYMLGVDRKEFMTKKKPAVEGGS"
BASE COUNT          425 a          752 c          707 g          373 t
ORIGIN      
        1 agatcacccc cagcgagcgc gccactccat cgaagtgggc gcagagcgcc gcgacatcct
       61 cggcgagtgc tgcggcggga ggcggcgcac gcgggagcgc cgcgttgatc gcgaggagaa
      121 cctcgtaggc gatgacctgc acggccgccg cgagattgag cgagccgcga tcgccggccg
      181 tcggaatgta ccagagccgg ttcgcacgat cgagtgcgtc gttgtcgaga ccgcttcgct
      241 ccggaccgaa gagaatcgcg acatcgcgcg ccgcgagttc cgccacgagt tcggcggccc
      301 catcgcgcgc cgcgaccacg gccggcccca gatgacgcgg ccgtgcgctc gtcgcgacga
      361 cgaaacccgc gcccacgatc gcctcatcga gcgtgtcgac gcaggaggct ttctccagca
      421 cgtcgagcgc gcctcgtgcg cgcgcccgcg cctcctcgcc gatttcgcac tgcggcgcaa
      481 ccagaacgag actcccgagt cccattacct tcatcgcgcg cgccgccgca ccgacgttcc
      541 ccggatgcga agtcgcgacc aggacgatgc ggatggttcc ggacatgaag cagagatttt
      601 ccgcgggcct ttgctattct aggcgcaagg cccgctccga atgcttccga tcccgtggat
      661 ccgctcgcca acatcgccgt gagcgccgcc cgccgtggcg gtgcggtcat tctgcgatcc
      721 ctcgaccgtc tcgacctgct gaaggtggag acgaaacgcc acaacgattt cgtcaccgag
      781 atcgatcgcc gctccgaggc gaccatcatc gagacgattc gccgggccca ccccgatcac
      841 gcgattctcg ccgaagaacg cggccgcgaa gacaccggca gcgccatcga atggatcatc
      901 gacccgctcg acggcaccac caattatctc cacgggtttc cggccttttc cgtttccatt
      961 gcggtcagcg aacgcgggcg actcgtgacc ggcgtcgtct acgatcccct gcgcgaagaa
     1021 atgttcgtag cgagccgcgg gcagggcgct ctcctcaacg agcggcgcat tcgcgtcgcc
     1081 gcgcgcccgg cgctcgaagg cgcgctcgtc ggcaccggct ttcccttccg cgagcttcgc
     1141 gatctcgacg actatctcgc gatgtttcgc gccgtgatcg agcgtactag cggcctgcgc
     1201 cgcgccggtt cggcggcgct cgatctcgcc tggacggctg ccggacgtct ggacgggttt
     1261 ttcgaactcg gcctcgaggt ctgggacatc gccgcgggct gcctcctgat cgaggaagcc
     1321 ggaggaatca tcggagatgt gtgcggcaac gccggatggc ccatcgcgga agggaacatc
     1381 ctcgccggca atccgcgcat ctttgcggcg ctcgcccgcg cgctcgcgcc gcatctgcca
     1441 ccgcgcctcg cctcgcagcc ggcggcagag cgctccgatt cgcctcgtgc aaaaaccgcg
     1501 cgcgccgacc cgcttcgcaa atcaaaaagg aaataaggga atctttcatt gatttcgtca
     1561 taccggcgca gcccggtatc caatgaaaga aaaatcccgg ctctcgccgg ggtcacgggg
     1621 gaaaaaattg attcaaggtt cctgacgggg gtgcttcacc cgcagggaaa gcgctccgtc
     1681 aggaaccgcc ttccaccgcc ggtttctttt tggtcatgaa ttccttgcgg tccacgccga
     1741 gcatgtaggc cgaggcgctg gccacgtaga tcgaggaata ggtgccgacg acgatgccga
     1801 tggacaaggc gatcgagaac tcgcgcaacg gcggaccgcc gacgaccagc agcgcaatta
     1861 cgaccagctg cgtcatgacg tgagtcatga tcgtacgcga aagtgtctgg ttgacggcga
     1921 ggttgatgac ctcgtaggag ctgccgcggc gcacgcgctt gaagtcctcg cgaatacggt
     1981 cgaagatcac gacggtgtcg ttcaccgagt aaccgagcat cgcaagcacc gcggcgagca
     2041 cggtgagatc gaacttgaga tgggtaaacg agaatatccc gattatgaag acgacgtcat
     2101 gcagggtcgc gaagatggct ccgagagcga gccgccattc gtatcgaacg aggagatata
     2161 tgaaaatcag aataagggca acaaccaggg ccatcgcgcc ctgggtgcgg agctgactgc
     2221 cgacctgcgg ccctaccgtg ttcagctgct tttgccg
//
LOCUS       DASUJG010000055         2191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_627059, whole genome shotgun sequence.
ACCESSION   DASUJG010000055 DASUJG010000000
VERSION     DASUJG010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627059"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /gene="nth"
                     /locus_tag="VFX38_01070"
     CDS             <1..499
                     /gene="nth"
                     /locus_tag="VFX38_01070"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018169450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX5313487.1"
                     /translation="AATARLFAVANTPRAITQLGVNGLKPYVRTIGLYNSKATHIVAM
                     AQMLLAEHGGRVPGERTALEALPGVGRKTANVVLNTAFGEPVIAVDTHVFRVAKRIGL
                     ATGETPRAVEDELQRRTPSEYRRHAHHWLILHGRYVCKARRPECWRCLIRDLCEYPHK
                     TPAPS"
     gene            602..1270
                     /locus_tag="VFX38_01075"
     CDS             602..1270
                     /locus_tag="VFX38_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009150704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEX5313488.1"
                     /translation="MTVLDVNFKAARRQMVDQQIRAWGVLDPRVVAVMGNIARERFAP
                     ERYRYVAYADSNIPIGHGEVMLVPKVQGRLLQALELEEGHRVLEIGTGTGYLTACLLA
                     LGAAVRSVDIHEDFVRTATQRLEAEGFPDAEILQEDALRLAATAPEYDAILVTGSLPV
                     ADPRFPRLLAVGGRLVWILGQEPAMRAEVLVRLGDDEWRSHGLFETVAPALQGAAVPR
                     RFDF"
     gene            1329..>2191
                     /locus_tag="VFX38_01080"
     CDS             1329..>2191
                     /locus_tag="VFX38_01080"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX5313489.1"
                     /translation="MLRQLLLASGLVLAAAPAAYPADLWQVFQLAQSNDPAFREALAN
                     RNASMQAKPAAWANIFPSIDLNASRTWDNTHGDRLSFFGTGVFPVQSSSNDRNDQWGA
                     QLTQTLFNWQQFQALKGANFTVAQAEATYQSTLQGLIVTVSQAYFNVLNARDVLNADI
                     ANAKSLGKQYQQTQEQYKVGLIAVTGVREAEAGYDQARAQVIADRQALAQAEEALRAI
                     TGQYIGKLAEPQKKLPLHPPQPESVDTWVAQGLKENPSLIASQFGTKVAQNQVAQQRG
                     GYMPQLNLVLQ"
BASE COUNT          406 a          739 c          687 g          359 t
ORIGIN      
        1 cgcggcgacg gccagactgt ttgcggttgc taatactccc cgggcaatca cgcaattggg
       61 ggtcaatgga ctcaagccgt atgtccgcac gatcgggctc tacaacagca aggcgaccca
      121 catcgtcgcc atggcgcaaa tgctcctcgc cgaacacgga ggtcgcgtac cgggcgaacg
      181 cacggcgctc gaggccctgc cgggcgtcgg cagaaagacg gccaacgtcg ttctcaacac
      241 cgcattcgga gagcccgtca tcgcggtgga cacgcatgtg tttcgcgtcg caaaacgcat
      301 cggactcgcg acgggcgaaa cgccacgcgc ggtggaagac gaactccagc ggagaacgcc
      361 gtccgagtat cggcggcatg cccatcactg gctgattctc cacggccgct acgtctgcaa
      421 ggcgcggcgg cccgaatgct ggcgctgcct gatccgcgac ctttgcgagt acccgcataa
      481 gactccggcg ccgtcgtgac cgacacacgc cggccgcccg agagcgggcc cccgggaacg
      541 tagcttccgc gcggcacgct cgcgctatct gggataatgg cggcaccgcg gaggctccgg
      601 gatgaccgtg ctcgacgtga acttcaaggc ggcgcgtcgc cagatggtgg atcagcagat
      661 ccgcgcctgg ggcgtgctcg atccacgcgt ggtcgcggtc atgggcaaca ttgcccgcga
      721 gcgcttcgcg ccggagcgct atcgctatgt cgcctacgcc gactccaaca tcccgatcgg
      781 ccacggcgaa gtcatgctgg tgccgaaggt gcagggccgc ctgcttcagg cgctggagct
      841 cgaggaaggc catcgcgtgc tggaaatcgg aaccggcacc gggtatctca cggcctgcct
      901 gctcgcgctc ggcgcagccg tgagaagcgt cgacatccac gaagacttcg tccgcaccgc
      961 cacgcagagg cttgaggccg agggcttccc ggacgccgaa atcctgcagg aggacgccct
     1021 ccgactcgcg gccacggctc cggagtacga tgcaatcctc gtgaccggct cgcttccggt
     1081 tgccgatccg cgttttccgc ggcttcttgc cgtcggcggt cgcctcgtgt ggatcctcgg
     1141 gcaggaaccg gccatgcgcg ccgaggttct cgtccgtctc ggcgatgacg aatggcgcag
     1201 ccacgggctt ttcgagaccg tagcgcccgc cctccagggg gcggcggtgc cgcggcgctt
     1261 cgatttctga acgattgtcc ttcccttgaa atggtgttat agttcaccaa aacacggtgc
     1321 ccaaatccat gctccgacag ctcctgctcg cctccggcct cgtcctcgcg gccgcgccgg
     1381 ccgcctatcc agccgacctc tggcaggtct tccagctcgc gcagtcgaac gatccggcgt
     1441 ttcgcgaggc gctcgcgaac cgcaacgcct cgatgcaggc gaagccggca gcctgggcaa
     1501 acatatttcc ttcgatcgat ctcaacgcga gccgtacctg ggacaacact cacggcgatc
     1561 ggctgagctt cttcggaacc ggcgtgtttc cggtgcaaag ctcctccaac gatcgcaacg
     1621 atcaatgggg cgcgcaactg acgcagacgc tcttcaactg gcagcagttt caggccctca
     1681 aaggtgcgaa tttcaccgtc gcgcaggcgg aggcgaccta tcaatcgaca ctccaggggc
     1741 tgatcgttac ggtttcgcag gcctatttca acgtcctcaa tgcgcgtgac gtgctcaacg
     1801 ccgacatcgc caacgccaag tcgctcggca agcaatacca gcagacgcag gagcagtaca
     1861 aggtcgggct gattgccgtc accggcgtgc gcgaggccga ggccggttac gatcaggccc
     1921 gcgcgcaggt gatcgcggat cgccaggcgc tggcgcaggc cgaggaggcg ctgcgcgcga
     1981 ttacaggtca gtacatcggc aagctcgcgg aaccacagaa gaagctgccg ctgcacccgc
     2041 cgcaaccgga aagcgtggat acctgggtcg cgcagggact caaggaaaat ccttcgctca
     2101 tcgcctctca attcggcacc aaggtcgcgc agaatcaggt ggcgcaacag cgcggaggct
     2161 acatgccgca gctcaatctc gtgctgcagc a
//
LOCUS       DASUJG010000056         5901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_383479, whole genome shotgun sequence.
ACCESSION   DASUJG010000056 DASUJG010000000
VERSION     DASUJG010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5901
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383479"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VFX38_01085"
     CDS             <1..572
                     /locus_tag="VFX38_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009022948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5313490.1"
                     /translation="TMNTLIGQGKVLYWGTSEWSAEEIRAACDCARRLGLEGPTMEQP
                     QYNLFHRQRVEEEYASIFRDFGIGTTTWSPLASGILSGKYADGIPAGSRFDLEGYGWL
                     REMVESERGRARIEAASKVAAIARELDATPAQLAIAWCLRNPNVSTVILGASRPEQLR
                     QNLDALEVYERLDETVVTRLEELTAPVAD"
     gene            884..1843
                     /locus_tag="VFX38_01090"
     CDS             884..1843
                     /locus_tag="VFX38_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5313491.1"
                     /translation="MSNKIQLEPEAQEFVEASANPPYLADLGPEKGRDAVDEVQSGEI
                     SKPDVDIENITVAGKPGTQVAVKILRPKNARKPLPVVVYIHGAGWVFGNAHTHDRLVR
                     ELAVGADAAVVFPEYSLSPEAKYPTAIEESYAVTQWVAENGREHGLDADRVVVAGDSV
                     GGNMATAVTLMAKERGGPAIHGQLLLYPVTEASFDSDSYHEFATGYFLRRDAMMWFWD
                     QYTTDPKERAQTTASPLRATDEQLRGLPEALVITAEADVLRDHGEAYASKLRQAGVRV
                     TAARFEGIIHDFMMINAMANTAATRGAMALSLAWLRERFQQST"
     gene            2169..4211
                     /locus_tag="VFX38_01095"
     CDS             2169..4211
                     /locus_tag="VFX38_01095"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEX5313492.1"
                     /translation="METRGSGDELHRQAGGAHPALTTNQGVPIADNQNSLRATPRGAT
                     LLEDFILREKITHFDHERIPERIVHARASAAHGYFELTHSLKPYTTAKILTEVGVKTP
                     LFTRISTVAGGAGSIDTPRDVRGFAVKFYTKEGNWDLVGNDIPVFFIQDAIKFPDLVH
                     AVKMEPDRGFPQAATAHDTFWDFISLTPESMHVILWAMSDRAIPRSLRMVEGFGIHSF
                     RLLDAGGHSTFVKFHWRPKLGLQSHVWDEAVKVAGADPDFHRRDLFEAIQAGHYPEWD
                     LAVQLFTEEDAEKFPFDHLDATKIIPEELVPLTVIGRMVLDRWPDNFFAETEQVAYCP
                     ANIVPGIDFSNDPLLQGRLFSYLDTQLIRLGGPNFHQIPINAPKCPFANLQRDGHMQM
                     QLPKGRVNYEPNSLQDDTPREASDGFRSFAEQADDGVKGRIRAESFADHYSQARLFFR
                     SQTEPEQSHIASAFVFELSKVETAHVREAIVGHLRHVDERLAKRVAAGLGLASMPPAP
                     PVAVKARDMESSPALQIIGKMKDTLEGRAIGILVDDGSDSGVIETLRRAAEDAGAMVK
                     IVAPRLGVELGGGKRLDADGQLAGTPSVVFDAVALVLSESAGKRLAKDSAAVDFVHDA
                     YGHLKAIAVDAGGRVLLQGAGIESDAGVVDAADRTAFISAAKTRQWQREASVRLLA"
     gene            4260..5144
                     /locus_tag="VFX38_01100"
     CDS             4260..5144
                     /locus_tag="VFX38_01100"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX5313493.1"
                     /translation="MDAIVNEHAGGRNRKQAQRLRETLPRLFAQVGLDVQIHWSAPDE
                     LSARIERLLDTGVRRLIVGGGDGSLSTAADRLAGTGAVLGVLPLGTFNHFARDLQLPI
                     QLDEAVAVLARGREIVVDVGEVNGRAFVNNASLGVYVRATGFRDAYRSRLGLSKWPAM
                     ALAMLRVFWSFPLMALNIDDARGAHAFRTPFVFVGNNAYLREGVAGINRESLSEGMLG
                     IVSSRQIGRFGLVRMGWSIVRGRFDDARMLEQRLTDAARIEVGKRRVWVALDGELVRL
                     SPPLEFRIRAGVLKVVAA"
     gene            5141..>5901
                     /locus_tag="VFX38_01105"
     CDS             5141..>5901
                     /locus_tag="VFX38_01105"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5313494.1"
                     /translation="MTCIVHLSDFHYGAEIAEVEAALRADLAEGDYDLVVVSGDLTQR
                     ARRSQFRRARTLLAAIPEPKVVVPGNHDIPLYDLPRRFSGALTRYRHYVCESSYPGYG
                     DEDVVVIGVNTGRPFLWKEGKIDETQIAQLRERLASQPSGRIRVLVTHHPLCIPSGRR
                     LRLHRWARRLLDGLGEFRPEMVLSGHLHFSHVLELPRAGSNRENRSLAVQAGTAISDR
                     RRGEPNTYNRIELGPAGVSIEVRGFDGGRFATRRRL"
BASE COUNT         1078 a         1895 c         1876 g         1052 t
ORIGIN      
        1 gcaccatgaa cacgctcatc gggcagggaa aggttctcta ctggggcact tccgagtgga
       61 gcgcggagga aatccgcgcg gcctgcgact gcgcgcgccg gcttggtctc gagggaccga
      121 cgatggagca accgcagtac aacctctttc atcggcagcg ggtcgaagag gagtatgcgt
      181 cgatctttcg cgacttcggc atcggcacca cgacctggtc gccgctcgcc tccggaatcc
      241 tgagcggcaa gtacgctgat ggtattcccg cgggctcgcg cttcgatctc gagggctacg
      301 gctggctgcg cgagatggtc gagagcgaac gtggtcgcgc gcgaatcgaa gccgcgagca
      361 aagtcgctgc gatcgcccgc gaactggatg ccacgccagc gcaactcgcg atcgcctggt
      421 gcctgcgcaa tcccaatgtc tcgacggtca tcctcggcgc ttccaggccg gaacaactgc
      481 ggcaaaacct ggacgcgctc gaagtgtacg agcgtctcga cgaaactgtc gtgacgcgtc
      541 tcgaagaact cacggcgccc gtcgccgatt gattggtacg gcgtgccgga acgtcgtgcc
      601 atccgaatta ctccgccgca aggcgtactc cttcctcttc gttgaaaagc gcgcctgctt
      661 atggcggtta gaccgccggg tagcgagatc tgaaatcccg gtcagtgttc tttttgcggc
      721 cgggccggcg cttgtcggaa cgagcgtaat cgtcagccgt gattgctgat atactcggct
      781 tgacacccgt gactcgacac gcgccctgag ccgtactcgc cggatttcgc gcgtgagcat
      841 ttttgttgcg gcacatttat cgcaaaaaga aggagagcac tgaatgtcaa acaagattca
      901 actcgagccg gaagcgcagg agttcgtcga ggccagcgcg aatccgccgt atctggccga
      961 tctcggcccg gaaaagggcc gcgacgccgt tgacgaggtt cagtcggggg aaatctcgaa
     1021 gccggatgtg gacatcgaga atatcaccgt tgccgggaaa cccggcacgc aggtcgcggt
     1081 gaaaatcctt cgtccgaaaa acgcgcgcaa gccgcttccg gtcgtggtct atattcacgg
     1141 cgcgggatgg gttttcggca acgctcacac gcacgatcgg ctggtacgcg aacttgccgt
     1201 cggcgccgac gctgcggtgg tgttccccga atacagtttg tcgccggaag cgaaatatcc
     1261 cacggccatc gaggaaagtt atgcggtcac gcaatgggtc gcggaaaacg gacgcgagca
     1321 tggtttggac gcggatcgtg tcgtggtggc cggcgacagt gtcggcggaa atatggcgac
     1381 ggccgtcacc ctgatggcga aagagcgcgg cgggccggcc attcacgggc aattgctttt
     1441 gtatccggtg accgaagctt ctttcgactc cgattcctat cacgagtttg cgaccgggta
     1501 tttcctgcgc cgcgacgcca tgatgtggtt ctgggatcag tacacgacgg acccgaagga
     1561 gcgcgcccag accacggctt ctccgcttcg cgccaccgac gaacagcttc ggggcctgcc
     1621 ggaggctctc gtcatcaccg ccgaggccga cgtgcttcgc gatcacggcg aagcgtatgc
     1681 gagcaagctc cgtcaggccg gcgtgcgcgt gaccgccgca cgtttcgagg gcatcatcca
     1741 cgatttcatg atgatcaacg ccatggcgaa cacggccgcg acgcgcggtg cgatggcgtt
     1801 gtcgctggcc tggttgcgcg aaagattcca acagtcaacg tagaccggga taagcgcagc
     1861 gttcccggca atccctgcaa gatgctacgt tcgtcgcagg gttttgcagg ccggcataag
     1921 accgcagatc gttgccggca ttgcaagaat ttccggaacc tctgacccgg gcggaggtct
     1981 tcccatacga gtcagtccga caagcgggcc tcgaaccgga attttcagac gtgccgccga
     2041 agcacaggca gggcgtaaat cgcgggtacc aaggcgcggt ttatacaagg aggatcatca
     2101 tggccaaatc aaccgacccg aagcgacccg gccggccccc ggccgggaaa cggcgcggca
     2161 aggcaaatgt ggaaacccgc ggcagcggcg acgagctgca ccggcaggca gggggcgcgc
     2221 atccggcgct gacgacgaat cagggcgtcc cgatcgcgga caaccagaat tcgctgcggg
     2281 cgacgccgcg cggtgcgacc ctgctcgagg acttcatcct tcgcgaaaag attacgcact
     2341 tcgatcatga acgcattccc gagcgcatcg tgcatgcccg cgcctcggcg gcgcacgggt
     2401 acttcgaact gactcactca ttgaagccat acaccaccgc gaagatcctc accgaggtcg
     2461 gcgtgaaaac ccctctgttt acccgcatct ccaccgtcgc gggcggcgcc ggctcgatcg
     2521 acacgccgcg cgacgtccgc ggcttcgcgg tgaagttcta caccaaggaa ggcaactggg
     2581 acctcgtcgg caacgacatc ccggtgtttt tcatccaaga cgcgatcaag tttccggacc
     2641 tcgtccatgc ggtaaagatg gagcccgacc gaggcttccc gcaggcggcg accgcgcacg
     2701 acaccttctg ggacttcatc tcgctcaccc ccgaatccat gcacgtaatc ctgtgggcga
     2761 tgtcggaccg cgcgatcccg cgctcgctgc gcatggtgga gggctttgga atccacagct
     2821 tccgcctgct cgacgccggc ggccacagca ccttcgtcaa gttccactgg cgtccgaagc
     2881 tcggtctgca atctcatgtc tgggacgagg ccgtgaaggt cgcgggcgcc gatccggact
     2941 ttcaccgccg tgacctcttc gaggcgatcc aggccggtca ctaccccgag tgggatcttg
     3001 ccgtgcagtt gttcaccgag gaggatgcgg agaagtttcc gttcgaccat ctcgatgcca
     3061 ccaagatcat tcccgaggaa ttggtgccgc tgaccgtgat cggccgcatg gtgctcgatc
     3121 gctggccgga caacttcttc gccgagaccg agcaggttgc ctactgtccg gccaacatcg
     3181 tgcccggcat tgatttcagc aacgatccgc tgctgcaggg ccggctcttt tcgtatctcg
     3241 acacgcaact gatccgtctc ggcggaccca acttccacca gattccgatc aacgcgccca
     3301 agtgcccgtt cgccaacttg cagcgcgacg gccacatgca gatgcagctg ccgaagggcc
     3361 gcgtcaacta cgagcccaat tccctgcagg acgacacccc gcgcgaagcg tccgacggct
     3421 ttcgcagttt tgccgagcag gctgatgacg gcgtcaaggg tcgcattcgt gcggaaagct
     3481 tcgccgacca ttactcgcag gctcggctct tctttcgcag ccagaccgag ccggaacagt
     3541 cgcatatcgc ctcggccttc gttttcgaac tgtcgaaggt cgagaccgca catgtacgcg
     3601 aggcgatcgt cggccacctg cgtcacgtcg atgaaagact ggccaagcgc gtcgcggccg
     3661 gtctcggcct ggcgtcgatg ccgcccgcgc cgcctgtcgc cgtcaaggcg cgcgacatgg
     3721 aatcatcacc cgccctgcag atcatcggca agatgaagga cacgctggaa ggacgcgcga
     3781 tcggcatcct cgtcgacgac ggttcggact ccggcgtcat cgagacgctt cgcagggccg
     3841 ccgaagatgc cggcgccatg gtcaagatcg tcgcacccag gctgggcgtc gaactcggcg
     3901 gcggcaagcg gttggatgcc gacggtcaac tggccggcac cccgtccgtc gtcttcgatg
     3961 cggtggctct ggtgctctcg gaatccgccg gcaagcggct cgcgaaggat tccgcggcgg
     4021 tggatttcgt gcacgatgcc tacgggcatc tcaaggccat cgccgtggac gccggtggtc
     4081 gcgtgttgtt gcaaggcgcc ggcatcgagt ccgacgccgg cgtggtagat gccgccgaca
     4141 ggaccgcctt catcagcgcg gccaaaacac ggcaatggca acgcgaagcc tcggtgcgtc
     4201 tgctggcata aacgctcccc cgccgacagg gcgactggtt ccttccgcgt gagatcgaaa
     4261 tggacgcgat cgtcaacgag catgccggcg ggcgcaatcg gaaacaagcg caacggctcc
     4321 gggaaacctt gccgcgcctg ttcgcgcagg tcgggctcga cgtgcaaatt cactggagcg
     4381 cgccggacga attgtcggcg cggatcgagc gactgctcga cacgggcgtg cggcgcctga
     4441 tcgtaggcgg cggcgacggc agcctgagca ccgcggccga tcggctcgcc ggaaccggcg
     4501 ccgtgctcgg cgtgctgccg ctcggaacgt tcaatcactt cgcccgggat ttgcaattgc
     4561 cgatccagct cgacgaagcc gtggccgtac tcgcgcgggg ccgcgagatc gtcgtggacg
     4621 tcggcgaggt gaacggccgc gcgttcgtca acaacgcttc gctcggcgtg tatgtacgcg
     4681 cgaccggatt tcgcgacgcc tatcggagtc gcctcggcct gagcaaatgg cccgcgatgg
     4741 cgctcgccat gctgcgcgtg ttctggagtt tcccgctgat ggcgttgaat attgacgatg
     4801 cccgaggcgc acacgccttc cgcacgccct tcgtgttcgt gggcaacaat gcgtatctgc
     4861 gcgagggagt ggccggcatc aaccgcgagt cgttgagcga aggcatgctg ggtatcgttt
     4921 cctcgcgcca gatcggacgc ttcggtttgg tccgcatggg ctggagcatc gtgcgcggcc
     4981 gcttcgacga tgcgcggatg ctcgagcagc gactcaccga cgcggcccgg atcgaggtgg
     5041 gaaaacgacg ggtatgggtg gccctcgacg gcgaactggt acggctttcc ccgccgctcg
     5101 aattccgcat tcgcgccggc gtgctgaaag tcgtcgcggc atgacctgca tcgttcatct
     5161 gtcggacttc cattacggag cggaaatcgc cgaagtcgaa gcggcgctgc gcgcagacct
     5221 tgccgaaggc gactatgatc tggtggtcgt cagcggcgac ctgacccagc gcgcgcgtcg
     5281 ctcgcagttc cgccgtgcgc gaacgctcct cgcggcaatc cccgagccca aggtcgtcgt
     5341 gccgggcaac cacgacatcc cgctctatga tttgccgcgc cgattttccg gcgcgcttac
     5401 ccgctaccgt cactatgtct gcgagtcgag ctatccgggc tacggtgacg aagatgtcgt
     5461 cgtgatcggg gtcaatacgg gccgcccgtt cctgtggaag gaaggcaaga tagatgaaac
     5521 ccagatcgcg caacttcgcg aacgcctcgc gtcgcagccc tcgggacgca tccgcgtgct
     5581 cgtgacccat catcccttgt gcatcccgtc cgggcggcgc cttcgtttgc accgctgggc
     5641 gcgacgcctg ctggacggac tgggcgaatt cagaccggag atggtgctct cgggacacct
     5701 gcatttcagt cacgtgctgg agcttccccg cgccggctcc aatcgggaga accgcagcct
     5761 ggccgtgcag gcgggcaccg cgatatccga tcgccggcga ggcgagccga acacctacaa
     5821 ccgcatcgag ttgggacctg ccggcgtttc catcgaggta cgcggattcg acggcggtcg
     5881 cttcgcgacg cgccggcgcc t
//
LOCUS       DASUJG010000057         2372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_627180, whole genome shotgun sequence.
ACCESSION   DASUJG010000057 DASUJG010000000
VERSION     DASUJG010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627180"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VFX38_01110"
     CDS             <1..249
                     /locus_tag="VFX38_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX5313495.1"
                     /translation="RMSPQLERPVWQPRFWEHTIRDEEDFCRHLDYIHYNPVKHGYAG
                     RPADWRWSSFRKFVRQGRYPPDWGADGDAHEIRNLDFE"
     gene            complement(375..>2372)
                     /locus_tag="VFX38_01115"
     CDS             complement(375..>2372)
                     /locus_tag="VFX38_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313496.1"
                     /translation="VCGGLQDNDGACGPSNSLDYAGIWGADWWDPIGGDGIYVVPAPS
                     DPAIVYADSEDGYSVRIDKRTMTSTFIRPTLASVNDTPTSKLQFRFNWGSPIAVSPTD
                     PNTVYIGSNVVFESSDGGANWKPISKDLTRNDKSHQPVAGGPVMHDISGAETSDTVLS
                     IAIAPTDPKVIWAGTDDGLVWVTKDGGAHWSNVTPGLPSSVKFGRIYQIGVSPFDAGT
                     AYLTVDGHMVGDNHPYVFRTGNYGTGWSGIAGGLPNDYAAMVVREDPNRKGLLALGTM
                     RGLYLSFDDGAHWTPMHANLPTMPVFDLKFTRSPQDLVLATHGRGLWILDNIEAIEQW
                     SPDLAKSDFHLFAPSPGIEWLGYFGRHIGPAPTDFVADNPPAGPQLAYYLESALTKGA
                     PAKAAASGSSPVTITVSDSAGNHVATLHGDGKAGINRVAWNMRYEGAKLPKFLQSGSF
                     GGGSGPNGPLALPGNYKVRIAAGKKNATTTVTVAADPRVHGSPGNQRALLQTGLEVRN
                     DVSALVEVLERIHGMVGTLGDVSDATAGAKKGSAQQQVNAAAARLKMQLGGFAMKLYN
                     PDLQFSAPEDSLHYVARFGMKLFGLYQNLSYVGPNQTLNAHQRREIAAAQAELAGYLA
                     KFNGPLRQAVVRFNQAAYKAGVQTLPVGKPISVKPVKIPAS"
BASE COUNT          391 a          719 c          813 g          449 t
ORIGIN      
        1 cgcatgtcac cgcagctgga aaggccggtg tggcaaccgc gtttctggga acacacgatt
       61 cgcgacgaag aggatttttg tcgtcatctg gattacattc attacaatcc ggtgaagcac
      121 ggatatgccg ggaggccggc cgattggcga tggagttcat tccggaaatt cgtgcggcaa
      181 ggccggtatc cacccgattg gggtgcggac ggtgacgcgc atgaaatccg gaatctggac
      241 tttgaatgat tgccggcaac gccgcttcgc ggcttatgcc ggcctacaaa gaacaaatgt
      301 aggcccgcat aagcgaagcg ttgcgcgtaa tcggagccgg atggcggcaa cgtcgcttcg
      361 ctccttgtgc cggcctacga ggcggggatt ttcacgggct tgacgctgat cggtttgccg
      421 acggggagcg tctgcactcc ggccttgtag gccgcctgat tgaatcggac gacggcctgg
      481 cgcagcggac cgttgaattt ggcgaggtag cccgcgagtt cggcctgcgc cgcggcgatc
      541 tcccggcgtt ggtgcgcgtt gagcgtctgg ttcggaccga cgtaggacag gttctgatag
      601 agcccgaaga gcttcatgcc gaagcgcgcg acatagtgaa ggctgtcctc gggtgcgctg
      661 aattgcagat cggggttgta gagtttcatc gcgaagccgc cgagttgcat cttgagcctg
      721 gcggccgcgg cgttcacctg ctgctgcgcg cttccctttt tcgcgcccgc ggtcgcatcg
      781 ctcacgtcgc cgagagttcc gaccatgccg tgaatgcgct cgagcacttc gacgagcgcg
      841 ctcacgtcgt tgcgcacttc gagccccgtc tggagcaggg cgcgctgatt cccgggcgag
      901 ccgtgcacgc gcggatcggc ggcgaccgtc acggtcgtgg tcgcgttctt ttttccggcg
      961 gcgatgcgga ctttgtagtt gccggggagc gcgagcggtc cgttcggtcc cgagccgccg
     1021 ccgaaggatc cgctttggag aaacttggga agcttcgcgc cctcgtaacg catgttccag
     1081 gcgacgcggt tgatgccagc cttgccatcg ccgtggagcg tcgcgacgtg attcccggca
     1141 ctgtcgctca cggtgatcgt caccggcgag ctgcccgaag cggccgcctt cgccggcgcg
     1201 cccttcgtga gtgcggactc gagatagtag gcgagctgcg gtcccgcggg cggattgtcg
     1261 gcgacgaaat cggtcggagc ggggccgatg tgacggccga agtagccgag ccattcgatt
     1321 cccggcgagg gcgcgaagag atggaaatcg ctcttcgcga ggtccgggct ccattgttcg
     1381 atcgcctcga tgttgtcgag aatccacagc ccgcgaccgt gcgtcgcgag caccagatcc
     1441 tgcggcgatc gggtgaactt cagatcgaag accggcatcg tcggaagatt ggcatgcatc
     1501 ggcgtccagt gcgcgccgtc gtcgaaggag agatagagtc cacgcatggt accgagcgcg
     1561 agcaaccctt tcctgttcgg gtcctcgcgc accaccatcg ccgcataatc gttgggcagg
     1621 ccgccggcga tgccgctcca gcccgtgccg tagtttccgg tcctgaaaac gtaggggtga
     1681 ttgtcgccga ccatgtgccc gtccacggtg aggtaggccg tgccggcatc gaagggggag
     1741 acgccgatct ggtagatgcg cccgaacttc accgaggagg gcagccccgg cgtgacattg
     1801 ctccagtgcg cgccgccgtc cttcgtcacc cacacgaggc cgtcgtcggt gccggcccag
     1861 atgaccttcg gatcggtggg cgcgatcgcg atcgaaagca cggtgtcgga agtctcggcg
     1921 cccgaaatgt cgtgcatcac ggggccgccc gcgaccggct gatgtgactt gtcgttgcgc
     1981 gtgaggtcct tcgagatcgg cttccagttc gcgccgccgt cgctggactc gaacaccacg
     2041 ttggagccga tgtacaccgt attcggatcg gtcggcgaga ccgcgatcgg cgagccccag
     2101 ttgaagcgga actggagctt cgaggtcggc gtgtcgttca cgctcgcgag cgtcgggcgg
     2161 atgaaggtcg aggtcatcgt gcgcttgtcg atgcgcacgg agtagccgtc ttcggaatcg
     2221 gcatacacga tcgcgggatc ggaaggcgcg ggcaccacgt agatgccgtc gccgccgatc
     2281 ggatcccacc agtccgcgcc ccagatgccg gcataatcga ggctgttcga ggggccgcag
     2341 gcgccgtcgt tgtcctgcag gccgccgcag ac
//
LOCUS       DASUJG010000058         2601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_750037, whole genome shotgun sequence.
ACCESSION   DASUJG010000058 DASUJG010000000
VERSION     DASUJG010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750037"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /gene="pdxA"
                     /locus_tag="VFX38_01120"
     CDS             <1..751
                     /gene="pdxA"
                     /locus_tag="VFX38_01120"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX5313497.1"
                     /translation="KPDPANAPAVLDSIREAVNLALDGRAAAVVTNPIHKAVLMTAGF
                     RHPGHTEFLGELCGLNEPPLMMLANPMLRVVLVTVHEPLKAAIAAITTARIVRVARIA
                     ATALKRDLGIEFPRIAVAGLNPHAGEEDRLGTEDRAIVTPAVAELKADGIAAEGPFPP
                     DTMFAAHARARYDVAVCLYHDQGLIPLKTLDFAHGVDVTLGLPIVRTSPDHGTAFDIV
                     GRGTADPSSMVAAIEMASEMAARRAKTVADA"
     gene            748..1602
                     /gene="rsmA"
                     /locus_tag="VFX38_01125"
     CDS             748..1602
                     /gene="rsmA"
                     /locus_tag="VFX38_01125"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX5313498.1"
                     /translation="MIARLPDGLPPLKTVIAEFGLAARRSLGQHFLLDPAITARIAAA
                     AGDIAGRTVIEIGPGPGGLTRALLAAGAAPLVAIERDERCVAALEDLAERAGERLVVV
                     GADAIAADEVALAHEAGGMAPLTIVANLPYNIATVLILKWLAHSADYADIVVMVQKEV
                     AARLAAAPSSAHYGRLSVMVQWRAEVATLFDLAPGAFTPPPKVVSTVIRITPRRVPLC
                     PADPAAIEKVVRAAFGQRRKMLRSALNVLGRNSGDLLRAAGIEPTRRAEELSVEDFCR
                     LARAYAAT"
     gene            complement(1605..2276)
                     /gene="gmk"
                     /locus_tag="VFX38_01130"
     CDS             complement(1605..2276)
                     /gene="gmk"
                     /locus_tag="VFX38_01130"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX5313499.1"
                     /translation="MSAKVTGESAVIDPATIHRRGLMLVLSSPSGAGKTTISRELLRN
                     EPNLMMSVSATTRAKRPGEVAGSDYIFVDTMAFNLMVNRREFLEHAQVFGNYYGTPRE
                     PVERALAAGRDVLFDIDWQGTQQLAENAREDLVTVFVLPPSTRELERRLHTRAQDSAD
                     IVAKRMARAADEMSHWIEYDYVIVNHDIETSVAAVRSILAAERLRRHRQIGMGDFVKS
                     LREGQ"
     gene            complement(2273..>2601)
                     /locus_tag="VFX38_01135"
     CDS             complement(2273..>2601)
                     /locus_tag="VFX38_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HEX5313500.1"
                     /translation="KERLTRQIAEIAGAAGVTPERIAQEVALLASRADVREELDRLTA
                     HVAQGRELLEASEPVGRRLDFLSQELNREANTLCSKSSDLALTRIGLELKAAIEQFRE
                     QVQNIE"
BASE COUNT          408 a          897 c          838 g          458 t
ORIGIN      
        1 aaaacccgat cccgccaatg cgccggccgt gctcgactcc atccgggaag ccgtgaatct
       61 cgcgcttgac ggtcgtgccg ccgccgtcgt caccaacccg atccacaagg cggtcctgat
      121 gacggcggga ttccgccatc ccggccacac cgagttcctg ggcgagctgt gcggactcaa
      181 cgagccgccg ctgatgatgc tcgctaaccc gatgctgcgc gtggtgctgg tcacggtgca
      241 cgagccgctg aaagccgcga ttgcggcgat tacgacggcc cggatcgtcc gtgtggcgcg
      301 aatagcggcg actgcgctga agcgcgacct cggcatcgag ttcccgcgga tcgccgtcgc
      361 cggcctcaat ccccacgccg gcgaagaaga tcgtctcggc accgaggacc gggccattgt
      421 cacgcccgcg gtcgcggagc taaaggccga cggcatcgcc gccgaaggcc cgtttccgcc
      481 ggatacgatg ttcgccgccc acgcgcgagc ccgctacgac gtcgctgtgt gcctgtatca
      541 cgatcagggt ctcatcccgc tcaagacgct ggacttcgcc catggcgtcg acgtgaccct
      601 gggtcttccc atcgtgcgca cctcgcccga ccacggcacc gctttcgata tcgtcgggcg
      661 cgggactgcc gatccgtcga gcatggtcgc ggcgatcgaa atggcctccg agatggcggc
      721 gcgccgcgca aagaccgtcg ccgacgcgtg atcgcgcgct tgccggacgg gctacccccc
      781 ctcaaaacgg tcattgccga attcggtctc gcggcacgcc gctcgctggg tcagcacttc
      841 cttctcgatc ccgcgatcac ggcgcgaata gcggcggcgg ccggcgacat cgccggacgt
      901 accgtcatcg agatcggccc gggcccgggc gggcttaccc gggccctgct cgcggcgggc
      961 gccgccccgt tggtggcgat cgagcgcgac gagcgttgtg tcgcggcact cgaggacctc
     1021 gccgagcgcg cgggcgagcg tctggtcgtg gtcggcgccg acgcgatcgc ggccgatgag
     1081 gtcgcccttg cccacgaggc cggcgggatg gcgccgctca cgatcgtcgc gaatcttccc
     1141 tataacatcg cgacggtctt gattctgaag tggctcgccc actctgcgga ttacgccgat
     1201 atcgtcgtca tggtgcaaaa ggaagtcgcg gcacggctcg cggccgctcc gtcctccgct
     1261 cattacgggc ggctttccgt gatggtgcag tggcgggcgg aggtcgcgac gctgttcgac
     1321 cttgctccgg gcgcgttcac gcccccgccg aaggtcgtct ccacggtgat ccggatcaca
     1381 cccaggagag tcccgctctg tcctgccgat cctgcggcaa tagaaaaggt ggtgcgggcc
     1441 gccttcggac aacgccgcaa gatgctccgc tcggcgctca acgtcctggg gcggaacagc
     1501 ggcgaccttc tgcgcgcggc cggaatcgag ccgacgcggc gcgccgaaga gctttccgtg
     1561 gaagatttct gccggctggc gcgtgcctac gcggcgacgt aactctactg tccctcgcgg
     1621 agcgatttca cgaaatcgcc catcccgatc tgccggtgcc gccggagccg ttccgcggcg
     1681 aggatgcttc gcaccgccgc gacgctcgtt tcgatatcgt gattgacgat cacatagtcg
     1741 tactcgatcc agtgactcat ttcgtcggcg gcccgcgcca tgcgcttcgc cacgatatcg
     1801 gccgaatcct gggcgcgggt gtgaagcctc cgctcgagtt cgcgcgtcga cggcggaaga
     1861 acgaacaccg tcaccagatc ttcgcgcgcg ttctcggcaa gctgctgggt tccctgccaa
     1921 tcgatgtcga acagcacgtc ccgtccggcc gccaatgcac gttcgaccgg ttcgcgcggc
     1981 gtgccgtagt agtttccgaa cacctgcgcg tgctccagga actcgcggcg attcaccatc
     2041 aggttgaagg ccatcgtatc gacgaagatg taatcgctgc cggcgacctc ccccggccgt
     2101 ttggcgcgcg tggtggccga gaccgacatc atgagattcg gctcgttccg caagagctcg
     2161 cgggaaatcg tggtcttccc ggcgcccgac ggcgaggaca ggaccagcat cagcccgcga
     2221 cggtggatcg ttgcgggatc gataacggcg gactccccgg tgactttggc gctcattcga
     2281 tgttttgcac ctgctcgcgg aattgctcga tcgccgcctt gagctcgagg ccgatgcgcg
     2341 tgagcgcaag atcggaggat ttcgagcaga gcgtgttcgc ttcgcgattc agctcctgcg
     2401 acaggaaatc gagccgacgc cccacgggtt cggaagcttc gagcaactct cgaccctgag
     2461 ccacatgggc cgtgaggcgg tccaattcct cgcggacatc cgctcgcgag gcgaggagcg
     2521 ccacctcctg cgcgattcgc tccggcgtta cgccggcagc acctgctatt tccgcgatct
     2581 gacgggtgag cctctccttg a
//
LOCUS       DASUJG010000059         4964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_506085, whole genome shotgun sequence.
ACCESSION   DASUJG010000059 DASUJG010000000
VERSION     DASUJG010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4964
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506085"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..1400
                     /locus_tag="VFX38_01140"
     CDS             150..1400
                     /locus_tag="VFX38_01140"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5313501.1"
                     /translation="MMFRPLPLAIGLRYLRVRRRSRFVSVVALVAVLGVALGVGALIV
                     VLSVMNGMQDTLTGSILGVTSDVTVATANGDALPELPRLVREARSERGVVATAPYAER
                     QIVLGRGEALAGAVLQGVQPQAQAQVSTLGRHMLLGSLAALAERPWAIVLGRDLAFQL
                     GVVPGDKLSVILPRGLVTPVGFVPRMRRFTVVGIFYSGSPEYDAGLALTSLSSAQKIF
                     EVDGASGLRLKLAEPFRAPEVAARLRAIVPAGVVVRDWQSEHQSLFAALANEQRMMFV
                     LVALAVLIAAFNVLGVLTVLVADKRSEIAVLASLGLPPRGILAAFLALGSTIGAIGIL
                     LGLGGGLAIAWNINAIVSTLGRLAGHPIFSAGAIAMPGLPSRISPEQVGIVVAIAAVL
                     VVIAAWVPARRAAHMRPVEALADG"
     gene            1393..2064
                     /locus_tag="VFX38_01145"
                     /pseudo
     CDS             1393..2064
                     /locus_tag="VFX38_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961838.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            2160..3383
                     /locus_tag="VFX38_01150"
     CDS             2160..3383
                     /locus_tag="VFX38_01150"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5313502.1"
                     /translation="MIVKLAWRLLFARGRLATLLAGVAVGGIALGVLVLTTVLAVMSG
                     FESVLSQKLVALGAEITVTPPNGNGDWQALAKRLEAQPQVVAAAPSLIGQALLLHDGQ
                     LAPVAVRGISAPDEARVSDLDTHLVAGKLSELRPGSRGLIVGEALAQTLKLQAGDSLT
                     LLTARAGRGDFGFTPALARYKVLGIYRLGIYKLEQSQVYTDLADARALFPEAGAASVA
                     LRLAQPLEAPAVAGHLRAYLGARYAISDWTQNQADLFATIGLEKRMLFVVLAAILAVA
                     AFTVVATLLVSGLDREPDIAVLKTLGFTPRRIASVFLVQGLVLGAVGTAIGLGLGAAL
                     ALNVNRLLGALDHVFHTQLLPPSIYLISELPARFHWQEILATAIVAIVLSLAAAVYPA
                     LQSARRAPAEALRYE"
     gene            complement(3536..>4964)
                     /locus_tag="VFX38_01155"
     CDS             complement(3536..>4964)
                     /locus_tag="VFX38_01155"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313503.1"
                     /translation="AYVFTDDNGIWSQTQKLVVSDGDTGDEFGWSVALDGRTALIGAP
                     YALNDDGSFPGAAYVFTEFGGTWTQTQRLVPDNGVDYDSFGYSVALSGTTALIGAIYA
                     GDDLNGAAYVFDKVDGSWHQRIELAPGDGSLFGGDEFGYSVAVGGDTAIVGAIFAGLN
                     AYDAHEGAAYVFSDANGEWDQTQKLSAGDATSDGFFGGSVALSDATALISAGGQGSAY
                     IFNESDGVWAQTQELVLRGVYVALDGTNALVGTDLFSDLGGTWTQIQEFTQNDATDKQ
                     DFGTASALSGTAVLIGGDCYEGGPHPGECTGPGAAYFYGEGDLDLAVSAPATVAQGQT
                     YESQAIATNDSSATSPAVVLSIAVPAAANFVSATASQGSCSAASGVVACDVGAIGGNA
                     GTATATVKFKATGSVGTKIENAAGVARALPSLSASAATMIEEGPPPPPPPPPSGGGGG
                     GGATGPFGLGLLGFVGLLAVLRRRR"
BASE COUNT          806 a         1750 c         1511 g          897 t
ORIGIN      
        1 acgaatacgg ccagagcctg cgcaatcacg ttccgcccca aggatccgcc gcggggaact
       61 atagcaccgt cgcgaagcgg cgggcgccct gaccccgagc ccggctccgg cgccgccctg
      121 tagaatcccg cgaggccctg atccgaagca tgatgttccg ccctctgccc cttgccatcg
      181 gcctgcgcta cctgcgcgtg cgcaggcgca gtcgtttcgt gtccgtggtg gcgctggtcg
      241 ccgtgctcgg cgtcgcgctc ggcgtcggcg cgctcatcgt cgtgctgtcg gtgatgaacg
      301 gcatgcagga cacccttacc ggcagcattc tcggagttac cagcgacgtg acggtcgcaa
      361 ccgcgaacgg cgacgcgctg ccggagcttc ctcgccttgt ccgcgaagca cgaagcgagc
      421 gcggcgtcgt cgccacggct ccctacgccg aacgtcagat cgtgctcgga cgcggcgagg
      481 ccctcgccgg cgccgtgctg caaggcgtgc agccgcaagc ccaggcgcaa gtctcgactt
      541 tgggccggca catgcttctc ggctcgctcg ccgcgctcgc cgaacggccc tgggccatcg
      601 tgctcgggcg cgatctcgcc ttccagctcg gagtcgttcc cggcgacaaa ttgagcgtca
      661 ttctcccgcg cggcctcgtg actccggtcg gtttcgtgcc gcgcatgcgc cggttcacgg
      721 tggtcggaat cttctattcc ggcagcccgg aatatgatgc cgggctcgcc ctgaccagcc
      781 tttcctcggc gcaaaagatc ttcgaggttg acggcgcgag cggactgcgc ctgaagctcg
      841 ccgaaccctt ccgcgcaccc gaggtggccg cacgcctgcg ggcgatcgtc ccggcgggag
      901 tcgtcgtgcg cgactggcaa tcggaacacc agagtctctt cgccgccctc gccaacgagc
      961 agcggatgat gttcgtgctg gtcgcgctcg ccgtactcat cgcggctttc aacgtgctcg
     1021 gtgttcttac cgtgctcgtc gccgacaaac gcagcgagat tgccgtcctc gcgagtctcg
     1081 gcctgccgcc gcgcggcatt ctcgcggcct ttcttgctct cggcagcacg atcggcgcga
     1141 tcggaatcct tctcggcctc ggcggcggac tcgcgatcgc ctggaacatc aacgcgatcg
     1201 tgagcacgct cggccgtctc gccggacatc cgattttttc cgcaggcgcg atcgcgatgc
     1261 cgggtctgcc ctcgcgcatc tccccggagc aggtcgggat cgtcgtcgcc atcgcggccg
     1321 tgctggtcgt gatcgcggcc tgggtgccgg cgcgccgcgc cgcgcacatg cgcccggtcg
     1381 aggcgctcgc cgatggctga gccggtgctt gtcggccgcg gactggaaaa acgctatcgt
     1441 cagggcggcg acgagcttgc ggtccttcgc ggcatcgatc ttgccgtcgc ggcaggcgaa
     1501 cgcatcgcga ttctcggcgc ctcgggaagc ggcaagtcca cgctgcttca tctcctcgcg
     1561 ggcctcgacc gtccgagcgc gggcgaagtc tttctcgcgg gcgaacgact cgaccgactc
     1621 gacgccggcg ccgctgcgcg ccttcgcaac cgcgcgctcg gcttcgtgta ccagtttcat
     1681 catctgctga tggaattctc cgccgtggaa aacgtcgcga tgcctctcct gattgcgggc
     1741 atgtcaaggc ggcgtgcgcg gcaggaagcc gaatccctgc tccggcgcgt cgggctcgca
     1801 aagcgaagcc acgcgcgtcc ggcaaaactg tcgggcggcg aacgccagcg ggtcgccatc
     1861 gcccgcgcgc ttgcgacgcg cccgctggtg gtacttgcgg acgagccgac cggcaatctc
     1921 gatgcggcga tcggcggcga agtctatgcg ctgatgctcg acatcaatcg cgaattcggc
     1981 accgcgtttc tcatcgccac ccacgatgcg atgatcgccc gcggcgcgga gcgcgtgttg
     2041 cgcctcgagc agggccggct cctcgcagca ggcagagatt tgggtaggag cgagcttgct
     2101 cgcgattccg aactggccgg cgggacgccg tcgctcccgc cctcgatccc ggacgcaagg
     2161 tgatcgtcaa actcgcctgg cgcttgctct tcgcccgcgg ccggctcgcg acgctcctcg
     2221 cgggagtcgc cgtcggaggg atcgcgctcg gcgtgctggt gctcacgacc gtgctcgcgg
     2281 tgatgagcgg cttcgaatcc gtcctgtcac agaaactcgt cgccctcggc gcggaaatca
     2341 cggtgactcc tccgaacggc aacggcgact ggcaggcgct cgccaagcga ctcgaagcac
     2401 aaccgcaagt cgtcgccgcg gcgccatcgc tgatcggaca ggcccttctt ctccatgacg
     2461 gccagctcgc gccggtcgcc gtgcgcggaa tctcggcccc ggacgaggct cgcgtttccg
     2521 atctcgacac gcatctcgtc gcgggcaaac tttccgaact caggccggga agccgcggcc
     2581 tcatcgtcgg cgaggcgctc gcccaaaccc tgaagctgca agcaggcgat tcgctcaccc
     2641 tgctcaccgc gcgcgccgga cggggcgact tcggttttac gccggcgctc gcaagataca
     2701 aggtgctcgg gatctatcgg ctcggcatct acaagctcga acaaagtcag gtctacacgg
     2761 accttgcgga tgcccgcgcg ctctttcccg aggcgggcgc ggcatcggtc gcgttgcgcc
     2821 tcgcacagcc gcttgaggcg ccggcggtcg ccgggcatct gcgcgcgtat ctcggagccc
     2881 gctatgcaat atccgactgg acgcaaaatc aggccgacct tttcgccacc atcggtctcg
     2941 aaaagcgcat gcttttcgtc gtgctcgccg ccatcctcgc ggtcgccgca ttcaccgtcg
     3001 tcgcgaccct gctggtctcg ggcctcgatc gcgaaccgga catcgccgtg ctcaagacgc
     3061 tcggcttcac tccacggcgc attgcctcgg tgttcctcgt gcagggactg gtgctcggtg
     3121 cggtcggcac cgcgatcgga cttggactcg gcgccgccct cgcgctcaac gtcaaccgcc
     3181 tgctcggcgc gctggaccac gtctttcata cccagcttct gccgccgtcg atctatctca
     3241 tcagcgagtt gccggcacgg ttccactggc aggaaattct cgcgaccgcc atcgttgcga
     3301 tcgtcctgag tctcgccgca gcagtctacc ccgcgctcca gagtgcccgc cgcgcccccg
     3361 ccgaagccct ccgctacgaa taagcaacct cttcattcat tccgcaatgt aggcctgcat
     3421 aagccgcgaa gcggcgttgc gggcaattca aaccggcaat aaggctgcgt tgccgccggg
     3481 aacgctccgc ttatcccggc ctacatgacg tcccgaaacc gctcaatcgg cctgctcagc
     3541 gtcttcggcg caggaccgcg agcagtccaa caaaaccgag caagcccaat ccgaatgggc
     3601 cggttgcacc tcccccaccg ccgccaccag atggcggagg cggcggcggt ggtggagggc
     3661 cttcctcgat catggtcgca gcactcgcac tgagcgacgg caatgcccgc gcgacgcccg
     3721 ccgcgttctc gattttggtg ccgacgcttc ccgttgcttt gaacttgacg gttgccgtcg
     3781 ccgtgccggc gttgccgccg attgcgccga cgtcacaggc gacgacgccg gaagctgcgc
     3841 tacaactgcc ttgcgatgcg gtcgccgaca cgaaattcgc ggccgcgggc acggcgatcg
     3901 acagcacgac cgccggcgaa gttgcgctcg aatcattcgt cgcgatcgcc tgactctcgt
     3961 aagtctgtcc ctgcgctacc gtcgccggcg cactcaccgc caagtcgaga tcgccctcgc
     4021 cgtagaaata cgccgcaccc ggccccgtgc actcgccggg atgtgggccg ccctcgtaac
     4081 agtcccctcc aatcaacacc gccgtacctg acagagcgct cgcagtaccg aagtcctgct
     4141 tgtccgttgc atcgttctgg gtgaattcct ggatctgcgt ccaagttcca cccaggtctg
     4201 agaaaagatc cgttccgaca agagcgttgg tgccgtcgag tgcgacgtag acaccgcgca
     4261 gcacaagttc ctgggtttgt gcccagacac catcggattc gttgaatata taggccgagc
     4321 cttgtccgcc cgcgctgatc aaagcggtcg catccgaaag tgctaccgat ccgccaaaga
     4381 aaccgtccga agtcgcatcg cccgcggaaa gcttctgcgt ctgatcccat tcgccgttcg
     4441 cgtcagaaaa tacgtaggcc gcaccttcgt gcgcatcata ggcattcagg ccggcgaaaa
     4501 tcgcaccgac gattgccgta tctccaccga cggcgaccga gtaaccgaac tcgtcaccgc
     4561 caaatagact gccgtcgccg ggagcgagtt cgatcctttg atgccaactg ccatcaactt
     4621 tgtcaaatac ataggctgcg ccgttcagat cgtccccggc gtagattgca ccgatcagcg
     4681 cagtagtacc cgaaagggcc accgaatagc caaaggaatc gtaatcgacg ccattgtccg
     4741 ggacgagccg ttgcgtttgt gtccaggtac ccccgaattc ggtaaacacg taggctgcgc
     4801 cagggaacga accgtcatcg ttcagagcgt aaggagctcc aataagcgcg gtcctgccat
     4861 cgagcgcgac cgaccagcca aactcatccc ctgtgtcgcc gtcgctgacg accagctttt
     4921 gtgtttgact ccatatcccg ttatcatcgg tgaatacgta ggca
//
LOCUS       DASUJG010000060         4768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1117800, whole genome shotgun sequence.
ACCESSION   DASUJG010000060 DASUJG010000000
VERSION     DASUJG010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4768
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117800"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VFX38_01160"
     CDS             <1..350
                     /locus_tag="VFX38_01160"
                     /inference="COORDINATES: protein motif:HMM:NF013430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313504.1"
                     /translation="KILPQVMVEGMAAGSVIVDLAAVSGGNCELSVADQTVVHHNVQI
                     LAPTNPAAAVATHASEMYARNCYNFVAPWFAEKGSLAIPADDEIYRATLVAGKFPSAS
                     PPPDLVLEPDPQP"
     gene            347..646
                     /locus_tag="VFX38_01165"
     CDS             347..646
                     /locus_tag="VFX38_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017942675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEX5313505.1"
                     /translation="MMIYGFVALYIFMLAAFTGYEVISRVPSILHTPLMSGSNFIHGV
                     VLVGAIIALGTAHGTLGRAIGFIAVVLAAANVAGGWVTTERMLAMFKSSKDEPRK"
     gene            643..2013
                     /locus_tag="VFX38_01170"
     CDS             643..2013
                     /locus_tag="VFX38_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX5313506.1"
                     /translation="MSAGQWIIQAAYFIAAILFIVGLKGMSSPRRARRGFYIAGIGML
                     TAVIATFFLSGVGGTTDIILILIALGLGGSISWIAGKRVPITAMPQMVALYNGMGGGA
                     AAAIAAHALVRGEQGTSEIVVAVVGALIGAVSFSGSLIAFAKLQGWMRAWRFPYQNFA
                     NAGALVAVALFAALAIIFGNAGVLVVFFLVALALGVLVTVPVGGADMPVMISLYNAMT
                     GLAVGFEGYVFGNPAMIVAGMIVGAAGSLLTYLMAKGMNRSLANVLFAGFGETAIAGG
                     EIEGELKAIEANDAAAMMAYAGHVIIAPGYGMAVAQAQHKLWEFVKLLRDKGVKVSFA
                     IHPVAGRMPGHMNVLLAEAGVPYDLIHDLDEINEEFVNADVALVIGANDVVNPAAREN
                     KSSPIYGMPILNVDRAQNVIVCKRGQGRGFAGIENELFYRDNTRMLYGDAQETMGRLI
                     QSVKSY"
     gene            2051..2317
                     /locus_tag="VFX38_01175"
     CDS             2051..2317
                     /locus_tag="VFX38_01175"
                     /inference="COORDINATES: protein motif:HMM:NF019009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HEX5313507.1"
                     /translation="MNSRKIPRANTSRSARFPKINDPRCGAESPADARSTDVNASATL
                     GLALTDKAGGQLRKRWLTDNLSSIEAYNEGVIKEGVFSDGLRNF"
     gene            2430..2636
                     /locus_tag="VFX38_01180"
     CDS             2430..2636
                     /locus_tag="VFX38_01180"
                     /inference="COORDINATES: protein motif:HMM:NF013965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HEX5313508.1"
                     /translation="MPLRPATRMTGKGIKVLTPVFAIAGKQYVMLTPQLAGVPRRALG
                     EQVGNLTSSRDRIIAALDLLITGI"
     gene            3215..>4768
                     /locus_tag="VFX38_01185"
     CDS             3215..>4768
                     /locus_tag="VFX38_01185"
                     /inference="COORDINATES: protein motif:HMM:NF025670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HEX5313509.1"
                     /translation="MFYQALAKDAGATYNINKQGCAVLPKQKLEGCFDRAGADFTGTT
                     ALRLHLVAFGRGNNLASITAVQPVIEANEASYAHGDLTEWWRVLPIGFEQGFTIAKRP
                     SGDGYLTLALAASRQDAGAPTISHSREGGNPVKTSPSIPLRSEGGKEIAWGNLRYGQL
                     VVTDANGKVVPATFRVAQDREQARSRGAGERILIAVNDAHAAYPLTVDPLVWAEAQKV
                     TASDGGRDDQFGYAVALSGTTALVGAPGHSEGGGAAYVFGEAGGSWTQTAELLAQANV
                     GSLCGTSVALSGTRALLGCPDPGFPGGVVVFSESGGTWTRTAFLQPSDSEIQDDFGYS
                     VAFSGTTALVGAPGHEINQGVAYVFGDSGGIWAQTAELVASDGAAGDEFGWSVALDGS
                     TALIGAPSHDGQGTAYVFAPSGATWVQAAELAASDGAAGDQFGDSVALDGATALVGAY
                     NHVSQGAAYVFGESGGSWTQTAEFAASDGVAGDEFGYSVALSGTTAFVGAPYSNYAQG
                     AAYDFDESGG"
BASE COUNT          830 a         1561 c         1510 g          867 t
ORIGIN      
        1 cgaagattct gccgcaggtc atggtcgaag gcatggccgc gggatcggtg atcgtggatc
       61 tcgccgccgt aagcggcggc aactgtgaac tcagcgtcgc ggaccaaacg gtcgtgcatc
      121 acaacgtgca gattctcgcg ccgaccaatc ccgccgccgc ggtcgccacg catgcgagcg
      181 aaatgtacgc acgcaactgc tacaacttcg tcgcgccctg gtttgccgaa aagggcagcc
      241 tcgcaattcc cgccgacgac gaaatctacc gcgcgacgct cgtcgccggg aagtttccct
      301 ccgcctcccc cccccccgat cttgttctcg aaccggaccc gcagccatga tgatctacgg
      361 cttcgtcgca ctttacatct tcatgctcgc cgccttcacg ggctatgaag tgatctcgcg
      421 ggtgccgagc attctgcaca cgccgttgat gtcgggatcg aacttcatcc acggagtggt
      481 gctggttggc gcgatcatcg cgctcggcac cgcgcacggc acgctcgggc gcgcaatcgg
      541 cttcatcgcc gtcgtgctcg ccgcggcgaa cgtggccggc ggctgggtca cgaccgagcg
      601 catgctcgcg atgttcaagt ccagcaaaga cgagccgcgc aaatgagcgc cgggcagtgg
      661 atcatccagg cggcgtattt catcgccgcc atactcttca tcgtcggcct gaagggcatg
      721 agttcgccca ggcgtgcgcg ccgcggcttc tatatcgccg gcatcggcat gctgaccgcg
      781 gtcatcgcaa ccttctttct cagcggcgtc ggcggcacga ccgacatcat cctgatcctg
      841 atcgcgctcg gcctcggcgg ctcgatctcc tggatcgccg gcaagcgcgt gccgatcacc
      901 gccatgccgc agatggtcgc gctgtacaac ggcatgggcg gcggcgcggc cgcggcgatc
      961 gccgcccacg cgctcgttcg cggcgagcag ggcacgagcg aaatcgtggt tgcggtcgtc
     1021 ggcgcgctca tcggcgccgt gtccttctcc ggcagcctga tcgcgtttgc caagttgcag
     1081 ggctggatgc gcgcctggcg cttcccgtac cagaacttcg cgaacgccgg cgcgctcgtc
     1141 gcggtcgcgc ttttcgccgc gctcgcgatc atcttcggca atgccggcgt gctcgtcgtg
     1201 ttcttcctcg tcgcgctcgc cctcggcgtg ctcgtgacgg tcccggtcgg cggcgccgac
     1261 atgccggtga tgatctcgct ctacaacgcg atgaccgggc tcgccgtcgg cttcgaaggc
     1321 tacgtgttcg gcaacccggc gatgatcgtc gccggcatga tcgtcggcgc cgcgggatcg
     1381 ctcctgacct acctcatggc gaagggcatg aaccgctcgc ttgcgaacgt gcttttcgcg
     1441 ggtttcggcg agacggcgat cgccggcggc gagatcgagg gcgaactcaa agccatcgag
     1501 gccaacgacg ccgcggcgat gatggcctac gccggtcatg tcatcatcgc gcccggttac
     1561 ggcatggcgg tcgcccaggc gcagcacaag ctttgggaat tcgtgaagct gttgcgcgac
     1621 aagggcgtca aggtgtcctt cgcgattcat cccgtcgccg ggcgcatgcc ggggcacatg
     1681 aacgtgcttc tcgcagaggc cggcgtgccc tacgatctga ttcacgatct cgacgagatt
     1741 aacgaggaat tcgtcaacgc cgacgtcgcc ctcgtgatcg gcgcgaacga cgtggtgaat
     1801 cccgccgcgc gtgaaaacaa atcgtcgccg atctacggca tgccgatcct gaacgtggat
     1861 cgggcccaga acgtcatcgt ctgcaagcgc ggtcagggcc gcggcttcgc cggcatcgaa
     1921 aacgaactct tctaccgcga caacacccgc atgctctacg gcgacgcgca ggaaaccatg
     1981 ggccgcctca tccagtccgt caaaagctac tgagccattg cgttacttgt aatgtccacc
     2041 aaaacgcata ttgaatagca ggaagatccc ccgtgccaac acatcacgtt ccgcgcgctt
     2101 cccaaagatc aacgaccctc gctgtggcgc tgaatcgcca gccgacgcca ggtcaacgga
     2161 cgtcaatgcg tcggcaactc tggggctagc tctgactgac aaagcaggcg gacagcttcg
     2221 gaaacggtgg ttgaccgaca acctttcttc cattgaggcc tacaacgaag gcgtcataaa
     2281 ggaaggagtg ttcagcgacg gattgcgcaa tttctgacac cgccattgga cgtccatgaa
     2341 agtgccgacg cggcaaaccc gagtaggctt tccccctgcc gctggacgta cagagcgacc
     2401 ttgtcgaaag tctcgacacg cggatcgtcg tgccgcttcg tcccgctaca cgcatgacag
     2461 gcaaaggcat caaggtgctt actcccgtct ttgctatcgc ggggaaacaa tatgtcatgc
     2521 tcacgcccca gctcgccggc gttcccaggc gggcgctagg cgaacaggtc ggcaatctca
     2581 cctctagccg cgaccggatc atcgccgccc tcgatctatt gattaccggc atctgagcta
     2641 ttgccggcag cggcttgtac ccttgtcggg gttcggatct gacactggcc tgccatttcc
     2701 gcattgaccg cgcaggatac ggtcagtctt cgaacgacat caatccggcc tcgtgtcatg
     2761 gaggcggagt cggtgggagc gggcctcgcc cgcgaccgtt tggctgcggt gccgatgttc
     2821 gtcgcgggcc gcgcgttccc gtacttgagg catggtgcga acgcttgacg ggctgcgggg
     2881 ccgagagtag attacgctcc ggtgcgtatc cgcgtgccga cccgagcctc cggcggacag
     2941 taaataagga tcccaacgcc gaccgctccg ccgccgacat gccctgcgag ccaggtgcgg
     3001 accccttccg atccgttcgc attattcgag aaacattcca gggagaactc cgatgcgcat
     3061 tcagggaaaa cttggttcgg gactgttctt gtttttgttg gttttggcgg gcggcggcgc
     3121 agtagcgctg acagcttcgc cgaacccacc tccgacgggc gccgcggccc gctccacgca
     3181 cacttctcag gcgctgcccg cggccctgcg cccggtgttt tatcaagctc tcgcgaagga
     3241 tgccggcgca acctataaca tcaataaaca aggttgtgcc gtattgccga agcagaagct
     3301 cgaaggctgc ttcgaccgcg ccggtgccga cttcacgggt acgacggcct tgcgcctgca
     3361 tctcgttgcc ttcgggcgcg ggaataatct ggcttcgatc acggcggtac aacccgtcat
     3421 cgaggcgaac gaagcgagct atgcccacgg cgatctcacc gaatggtggc gggtgctccc
     3481 gatcggcttc gaacagggct tcacgatagc caagcggccc agcggtgacg gctacttgac
     3541 gctggcgctc gccgcaagcc ggcaagatgc cggcgctccc acaatcagtc attcccgcga
     3601 aggcgggaat ccggtcaaga catccccctc gatccccctt cgttccgaag ggggaaaaga
     3661 aattgcgtgg ggaaatctac gctatgggca actggtcgtg accgatgcga acggcaaggt
     3721 cgtgccggcg acgtttcgtg tcgcgcaaga tcgcgagcaa gctcgctccc gcggcgccgg
     3781 cgaacgcatt ttgattgcgg tgaacgatgc gcacgccgcc tatccgctga cggtggaccc
     3841 gctggtgtgg gcggaggcgc agaaggtgac cgcgagcgac ggcgggagag acgaccaatt
     3901 cggctacgcg gtcgcgcttt ccggcaccac cgcactggtc ggcgcaccgg ggcatagtga
     3961 aggcggaggt gccgcctatg tattcggcga agcgggcggg agctggacgc agacggccga
     4021 actccttgcg caagccaatg tcggctcgtt atgcggcacc tcggtcgcgc tttcgggcac
     4081 gagagcgctc ctcggctgtc ccgatcctgg gttccctggc ggggtcgtcg tgttcagcga
     4141 gtcgggcggg acctggacgc ggacggcctt tctccagccg agcgacagcg agatccaaga
     4201 cgacttcggc tactcggtcg cattttcggg cactaccgcc ctcgtcggcg caccgggcca
     4261 tgaaattaac caaggtgtcg cctatgtgtt cggcgactcg ggcgggatct gggcgcagac
     4321 ggccgagctt gtggcgagcg acggcgcggc gggcgacgaa ttcggctggt cggtcgcgct
     4381 cgacggcagc accgcgctca tcggcgcacc gagccatgat ggtcagggca cggcttacgt
     4441 gttcgctccg tcgggggcca cctgggtcca ggcggccgaa ctcgcggcga gcgacggcgc
     4501 ggccggcgac caattcggcg actcggtcgc gctcgacggc gccaccgccc tcgtcggcgc
     4561 atacaaccat gtttctcaag gcgccgccta tgtgttcggc gagtcgggcg gaagctggac
     4621 gcagacggcc gagttcgcgg caagcgacgg cgtagcgggc gacgaattcg gctactcggt
     4681 cgcgctttcc ggcaccaccg ccttcgtcgg cgctccgtac agtaactatg ctcagggcgc
     4741 cgcctacgac ttcgacgaat cgggcggg
//
LOCUS       DASUJG010000061         5541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1117811, whole genome shotgun sequence.
ACCESSION   DASUJG010000061 DASUJG010000000
VERSION     DASUJG010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117811"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VFX38_01190"
     CDS             complement(<1..234)
                     /locus_tag="VFX38_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313510.1"
                     /translation="MLDSTREQDLDPEETREWLDSLRSVIAAEGLSRAHYLIEHLIDQ
                     ARRSGAYLPYTPNTAYLNTIAPSRQPQYPGNLEL"
     gene            387..1418
                     /gene="pyrC"
                     /locus_tag="VFX38_01195"
     CDS             387..1418
                     /gene="pyrC"
                     /locus_tag="VFX38_01195"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX5313511.1"
                     /translation="MRLTLARPDDFHLHLRDGAALADLVPPVARRFARALVMPNLDPA
                     VTTVAAAAAYRERIRAAIPRDCDFHALMTLYLTEATPAAEIERAATSGFVHAAKLYPA
                     GATTGSAAGVRDVAAIFPLLERMSEAGLVLAIHGELADPALDPYEREPRFVAEVLAPL
                     VGRFPELRIVLEHLSTRAAVEFVAAAGPNVAATITAHHLLCSRTEMFAQGLSPHHFCR
                     PVLQSEADREALLAAATSGNPKFFLGSDSAPHPRANKESARAAAGVYTGYAALELYAE
                     AFAAAGALERLENFASRFGAGFYRLPANSGSVVLEETSWQAPGELPLGAATVVPLGAG
                     ERLRFRLAE"
     gene            1415..2029
                     /gene="rnt"
                     /locus_tag="VFX38_01200"
     CDS             1415..2029
                     /gene="rnt"
                     /locus_tag="VFX38_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003282964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HEX5313512.1"
                     /translation="MNTLAERFRGYLPVVVDVETGGLDPATDALLEIALVLIEIESDG
                     ALRPAETLAMQVEPFAGARIVEKSLEITGIDPTHPLRPALPEHEALLRLFRPVRRALR
                     ESGCTRAVLVGHNAFFDLAFLNAAVARSDIKRNPFHPFTSFDTATLAGVFLGQTVLAR
                     ALEVAGIPHDPDAAHSAAYDAAVTAELFCLLVNRCRSVSRPYSL"
     gene            2144..2791
                     /locus_tag="VFX38_01205"
     CDS             2144..2791
                     /locus_tag="VFX38_01205"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX5313513.1"
                     /translation="MFFRPPPTTLSLIIANVAIFIIQQATGPFLVVHFALWPWGPHIA
                     LAAGTGTVFVGFEIWQLVTYAFLHGSIAHIFFNMFALYMFGSVIERTLGERRYLLYYF
                     VCVVAAALTHLLVVRFLTGGFYPTIGASGGIFGILLAFGMMYPRERVYFWFALPMPAW
                     VFVTGYGVLELVLGVTGTAEGIAHFAHLGGMIGGLPLMLYWMGKLPLKPRRRFPH"
     gene            2952..3575
                     /locus_tag="VFX38_01210"
     CDS             2952..3575
                     /locus_tag="VFX38_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313514.1"
                     /translation="MNVETKRPALKTSVPTALGAVVIFGLLMYLAAANPSPIAHRFGV
                     PSRNLPVWIVLAALWLGGYLWERQKRAAGASYAERMRRSVIVALAYFIPCIVGAVVAR
                     FLFPPEAVAVYSVWYWALSGLALLVTLFLAGIARSGHRCNKAAILVFASIMILAGVAS
                     APMFVFHAISQGSGMPQLAVVAVLLIAPPLLRDMGLFSTNVNHAETG"
     gene            3647..4045
                     /locus_tag="VFX38_01215"
     CDS             3647..4045
                     /locus_tag="VFX38_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313515.1"
                     /translation="MKRSETKKLIVMLNAGAFVAGAVFLFLAGSGRLKDNVGNQPIVI
                     AAACIAIAFLMICFYVLKTRMKRSSVRQNGRLLRTVAELTAMVAVGVGVYLLPTLLAP
                     GLAPMIWWALITTSSFLALLLVWSGRTRFE"
     gene            4176..5339
                     /locus_tag="VFX38_01220"
     CDS             4176..5339
                     /locus_tag="VFX38_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5313516.1"
                     /translation="MDKLDPEDLYAVGELLSDEERLARETTARFVDEKALPLIPEAFE
                     AGRFPDELIPEMAELGLFGSYFDDYGCAGLSGTAYGVICQELERGDSGLRSFVSVQTS
                     LVMFPIHEYGSDEQKSRWLPALAKGEAIGCFGLTEPQGGSDPGNMKTVAKKDGGDWLL
                     NGAKMWITNGTIADVAVVWARTEGGVRGFLVEKGMKGFDAPEVHHKMSLRASVTSALY
                     FDNVRVPEANVLPGVQSLRGPLSCLTQARYGITWGVIGAASACSREARNYVKERTLFG
                     RTLDHMQLTQARLAEMARGITAAQLLSWRLGQLKDQGNLHPTQVSLAKWNNARMAIGV
                     ARDARDLLGGAGITTEFSPIRHALNLESVITYEGTESVHQLAVGREITGINAF"
BASE COUNT          935 a         1792 c         1735 g         1079 t
ORIGIN      
        1 gagttcgagg ttgccgggat attgcggctg tcggctgggc gcgatcgtgt tcagatacgc
       61 cgtgttcgga gtgtagggca gatacgcgcc ggagcggcgc gcctgatcga tgagatgttc
      121 gatgagataa tgcgcacgcg aaagaccctc ggccgcgatt acggagcgca gcgaatccag
      181 ccattcgcgg gtttcttcgg ggtcgaggtc ttgttctcgc gtggaatcca gcatgaaagt
      241 tccttcactt cgggcggcga aacagccgcc ggacggccac ccggaaaacg gcaccccgcc
      301 ccggctccat cgcgggagcc tggaaaggcg accgggtacg attaccatta taggacgatg
      361 aatcagggga tcggcggaga aatcggatgc gtctgaccct cgcacgcccg gacgattttc
      421 acctgcatct gcgcgacggc gcggcgctgg cggatctcgt ccctccggtc gcccggcgct
      481 tcgctcgcgc cctcgtgatg cccaatcttg atccggcggt caccacggtc gcggcggccg
      541 ccgcctatcg cgaacgcatc cgcgcggcga ttccgcggga ttgcgacttc cacgcgctga
      601 tgacgctgta tctcaccgaa gcgacgccgg cggcggaaat cgagcgcgcg gccacgagcg
      661 gtttcgtgca cgcggccaaa ctctatccgg ccggcgcgac cacgggttcg gccgcgggcg
      721 tgcgcgatgt tgcggcgata tttccgctcc tcgaacgcat gagcgaagcg ggactcgtgc
      781 tcgcgatcca cggggaactc gccgatcccg ccctcgatcc ctacgagcgt gaaccgcgct
      841 tcgtcgccga ggtgctcgcg ccgctcgtcg ggcgattccc cgagcttcgc atcgtgctcg
      901 aacacctgag cacgcgcgcg gcggtggagt tcgtcgccgc ggccggaccg aacgtggccg
      961 cgacgatcac cgcgcatcat ctgctctgct cgcgcaccga gatgttcgca cagggtctct
     1021 cgccgcacca cttctgccgc ccggtgctcc agagcgaagc ggatcgtgag gcgctcctcg
     1081 ccgcggcgac gagcgggaac ccgaagttct ttctcggcag cgacagcgcg ccgcacccgc
     1141 gcgcgaacaa ggaatccgct cgggccgcgg ccggcgtcta taccggctac gccgcgctcg
     1201 aactctatgc cgaggccttt gccgcggccg gcgcactcga gcggctggag aacttcgcct
     1261 cgcgcttcgg cgccgggttt tatcgcctgc ccgcaaactc cggcagcgtc gtgctggagg
     1321 agacgtcatg gcaggcgccg ggggaactcc ccctcggcgc ggccaccgtg gtcccgctcg
     1381 gcgcgggcga gcgcctgcgc ttccggctcg ccgaatgaac acgctcgcgg aacgctttcg
     1441 cggttatctg ccggtggtcg tggatgtcga gaccggcgga ctcgaccccg cgaccgacgc
     1501 gctcctcgaa attgcccttg tgctgatcga aatcgaaagc gacggggcgc tccgtcccgc
     1561 ggaaacgctc gcaatgcagg tcgaaccctt tgccggcgcg cgcatcgtcg agaaatcgct
     1621 cgagatcacg ggaatcgatc cgactcatcc gctgcgtccc gcgcttcccg aacacgaggc
     1681 gctgctgcgc ctcttccgtc ccgtgcgcag ggcgctgcgc gagagcggct gcacccgcgc
     1741 ggtgctcgtc ggacacaacg cctttttcga tctcgcgttt ctcaacgcgg cggtcgcgcg
     1801 ctcggacatc aagcgcaatc cctttcatcc gttcacgagc ttcgataccg cgaccctcgc
     1861 gggggtcttt ctcggccaga ccgtgctcgc acgcgcgctc gaagtcgccg gcatcccgca
     1921 cgatccggat gccgcacatt cggccgccta cgatgccgcg gtgacggcgg aactgttctg
     1981 tctcctcgtc aaccgttgtc gctccgtatc acgcccctat agtctctgag catcgtcatg
     2041 accatgcaac gcgcgggtcg cacgcggcga gcggctccac ggcggcaacg acccatcgcc
     2101 ggcggcatcg gttagactcc cggaaacgac tctccggggc cgcatgttct tccgtccgcc
     2161 gccgacgacg ctctccctca tcatcgcgaa cgtcgcgatc ttcatcatcc agcaggcgac
     2221 cgggccgttc cttgtcgtcc acttcgcgct ctggccctgg ggcccgcata ttgcattggc
     2281 ggccggcacc ggcaccgtat tcgtcggctt cgagatctgg cagctcgtta cctatgcgtt
     2341 tctgcacggc tcgatcgcgc acatcttctt caacatgttc gcgctctaca tgttcggaag
     2401 cgtcatcgaa cgcaccctcg gcgagcgccg ctaccttctc tactacttcg tctgcgtcgt
     2461 cgccgccgcg ctcacgcatc ttctcgtcgt gcgtttcctc accggcggct tttatccgac
     2521 gatcggcgcc tcgggcggca tcttcggcat tctgctcgcc ttcggcatga tgtacccgcg
     2581 cgagcgcgtg tacttctggt tcgctcttcc gatgccggcc tgggtcttcg tcaccggcta
     2641 cggcgtgctc gaactcgtgc tcggcgttac cggcaccgcg gaaggaatcg cgcacttcgc
     2701 gcacctcggc ggaatgatcg gcggtctccc gctgatgctc tactggatgg gaaaactccc
     2761 gctcaaaccg cgccgccgct ttccgcactg acgagcctca cgcggcacgg caacacgggg
     2821 ccggccaccc agttcggttt ggacgcgccg cttcggccgg gggccttgtt ttaaggagcc
     2881 gcctggtgct aaacctgatg aggcccttca ggttagtggg tcccggtccg cgccaccatc
     2941 gagtttttta aatgaacgta gaaacgaaac gaccagcgtt gaagacttcg gtgccgacgg
     3001 cgctgggagc tgtggtcatt tttggtttac tcatgtatct ggccgcggcg aatccttcgc
     3061 cgatcgcgca tcgattcgga gtccccagcc gcaatttgcc ggtgtggatc gtgctggcgg
     3121 cactttggct tggaggctat ctatgggaaa ggcaaaaacg agcggcaggc gcctcttacg
     3181 ctgaaagaat gcgacgaagt gtaattgttg cgctggccta tttcattccc tgtatcgttg
     3241 gtgccgttgt cgctaggttt ttgtttccac ccgaggccgt agcagtctat tcggtctggt
     3301 attgggcctt gtccggattg gccttgctcg ttacgctttt tcttgccggt atcgctcgaa
     3361 gcggacaccg atgcaataag gcggcaatct tggtatttgc atcaatcatg attttggcag
     3421 gtgtggcctc cgcaccgatg ttcgtgttcc atgcaatttc tcaaggttcc gggatgcccc
     3481 agcttgcagt tgttgcggtg ttgttaatcg ctccgccgct gctgcgggat atgggcctgt
     3541 tctcgacgaa cgtcaaccat gccgaaacag gatagccgac cacacaaatg acattggcat
     3601 tgtcggttgc gcgggggatg caaacgccga cggcaccgag agaagcatga agcgtagcga
     3661 aacaaagaaa ctcatcgtaa tgctgaatgc gggtgccttt gtcgcaggag ccgtgttctt
     3721 gtttctcgcg ggtagcggac gtctaaaaga caatgtcggg aatcaaccga tcgtgatagc
     3781 cgcagcatgt atcgccatcg ctttcttgat gatttgtttc tacgtcttga aaacccgcat
     3841 gaagcgaagc agcgtgcgac agaatggacg actgttgcgt acagttgcgg aattgactgc
     3901 gatggtggcg gtcggagtag gagtctatct cctgcccacg ctacttgctc cgggattggc
     3961 gcccatgatt tggtgggcgt tgatcacgac aagctctttc ctcgcactgc tgctcgtttg
     4021 gtcgggccgg acacggttcg agtagtcggc gaaaatacag acgtgccata gttcgcttgg
     4081 aattggaatc caacgacctg cggggcgact ccccgaggga agccttctcc ttcgtgacgc
     4141 tgtaaaataa cgcgcttccc tgactggacg gccgcgtgga caagctcgac ccggaggatc
     4201 tttacgccgt cggcgaattg ctgagcgacg aggagcgcct cgcgcgcgag acgacggcgc
     4261 gcttcgtgga cgagaaggcg ctgccgctga tccccgaagc cttcgaggca gggcgtttcc
     4321 cggacgagct gattcccgag atggcggaac tcggtctgtt cgggagttac ttcgacgatt
     4381 acggctgtgc gggactttcg ggcaccgcct acggcgtgat ctgccaagaa ctcgaacgcg
     4441 gcgattcggg cctgcgcagc ttcgtctcgg tgcagacgag tctcgtcatg tttccgatcc
     4501 acgaatacgg ctccgacgaa cagaaaagtc gctggctgcc cgcgctcgcg aagggcgagg
     4561 cgatcggctg cttcggcctg accgagccgc agggcggctc cgatcccggc aacatgaaaa
     4621 ccgtcgcgaa gaaggacggc ggcgactggc tgctcaacgg cgcgaagatg tggatcacca
     4681 acggcacgat cgccgatgtc gccgtggtct gggcgcgaac cgagggcggc gtccgcggct
     4741 tcctcgtcga aaaaggcatg aagggtttcg atgcgcccga agtgcatcac aagatgtcgc
     4801 tccgggcctc ggtcacctcg gcgctttatt tcgacaatgt gcgggtgccg gaagcgaacg
     4861 tcctccccgg cgtgcaaagc cttcgaggcc cgctttcctg tctcacgcag gcgcgctacg
     4921 gaatcacctg gggcgtgatc ggcgccgcca gcgcctgctc tcgcgaggcg cgcaactacg
     4981 tgaaggagcg gacgctcttc gggcgcacgc tcgatcacat gcagcttacc caggcgcggc
     5041 tcgccgagat ggcacgcgga atcactgcgg cgcaattgct ttcctggcgt ctaggccagc
     5101 tcaaagatca gggaaacttg catccgacgc aggtctcgct cgccaagtgg aacaacgccc
     5161 gcatggcgat cggagtcgcg cgcgatgccc gcgaccttct cggcggcgcc ggcatcacca
     5221 ccgaattctc gccgatccgc cacgcgctca atctcgaatc ggtcattacc tacgaaggca
     5281 ccgaaagcgt gcatcagctc gcggtgggcc gcgagatcac gggcatcaat gcgttttgag
     5341 aaggcgatga agcgcggcag ggacaccccc ccgcgcggac gcggccccct tcgttccgaa
     5401 ggcggcgaag gctgcgctcg tctgcgcgga gagcgccgag gctacttccc ccttccgcag
     5461 gaagggggat cgagggggat gccctgaccc gcgggaagac aacgacgggg ctggcgcgca
     5521 gcgcgacgcg cgccatcaat c
//
LOCUS       DASUJG010000062         2226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_262124, whole genome shotgun sequence.
ACCESSION   DASUJG010000062 DASUJG010000000
VERSION     DASUJG010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262124"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFX38_01225"
     CDS             <1..258
                     /locus_tag="VFX38_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313517.1"
                     /translation="ERGLVAGALLLVLGVVLGIFAVGFWGVADFGALEPTHTMRFAIP
                     SATCITLAFEIVYGSFVLSVLGIREKEPERAPGHIGAAQPA"
     gene            328..1509
                     /locus_tag="VFX38_01230"
     CDS             328..1509
                     /locus_tag="VFX38_01230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5313518.1"
                     /translation="MRGGKWRDAHPATVRCQKIDRVLSRGLPGPRHQNCRIPEPELRF
                     SLFFEGVSAIRDPNMAATAATTGIPVGPPVQFPPSTPSRPFLSVMIPASAETDHLLET
                     LESVLAQDLGPGIMQIEVIDNSSAEGLGETLAERFRDRVSYFRHALRVSATRSWNTCL
                     ERARGEWTHILLANDVVRPDFYTTLRRTLEANPGVEAFACRCALTDDEGFWLGLSEPQ
                     AKVAGCLPAEFPIRQIVRHRLNLSSLVIRRDFFEQFGGFQERLRYCADWDLCNRLALK
                     RQIFYDPHFLVSVREHEGEGRFLYIPSGENVAEARECLRIYAALFPGNRRRTIYSDGM
                     TYAALDALRRCRHYRADGKAKGARRQLLEAGICMLGIARSYAMMPLRRFAIRETSTVG
                     R"
     gene            complement(1621..2211)
                     /locus_tag="VFX38_01235"
     CDS             complement(1621..2211)
                     /locus_tag="VFX38_01235"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5313519.1"
                     /translation="MTGSLEEPNPGADEEPTPLSPYAAAKWATCGYGRMFRSQYGLDV
                     RIVRPFMTYGPGQKEFKIVPSTILSLLHDRPAAIGSGSRLVDWIYVDDVIDGMLGIAA
                     AEDPPRRTIDLGSGDGATIGDVAREIARQIGKEHLLHIGDGARGREMVRLADTSTARR
                     YGFEPRVSLPDGLAATIIWFRQRKGSAANAHAVSAA"
BASE COUNT          361 a          726 c          726 g          413 t
ORIGIN      
        1 gagagggggc tcgtcgccgg cgcgctcctg ttggtcttgg gcgtcgtgct cggcattttc
       61 gcggtgggct tttggggggt cgccgacttc ggtgcgctcg agcccacgca caccatgcgc
      121 ttcgcgattc cgtcggcgac ctgcatcacg ctggcgttcg agatcgtcta cggttccttc
      181 gtcctgagcg tgctcgggat tcgggaaaag gaaccggagc gcgcgcctgg ccacataggg
      241 gcggcgcaac ccgcttagct gcgcgcctgc ggggccgtcg ctcccgctcg gcgccaaacc
      301 cgcagccggt gggatcgctc cgcgccgatg cgcggaggaa aatggcggga cgcccacccg
      361 gcaaccgtac gctgccagaa aatagaccgc gttctgtcac gtgggcttcc cggtcccagg
      421 caccagaatt gtcggatacc cgagcccgaa ctccgatttt ctcttttttt tgagggcgtt
      481 tccgcaatcc gggatccgaa tatggcggcc accgcagcga caacaggcat ccccgtgggc
      541 ccaccggtcc agtttcctcc ctcgacgcct tcgcggccgt tcctgtcggt gatgatcccc
      601 gcctcggccg aaaccgacca tctgcttgag actctcgaaa gcgtgctggc ccaggacttg
      661 ggccccggga tcatgcagat tgaagtcatc gataattcaa gcgccgaagg attgggggaa
      721 acgctcgccg agcggttccg cgatcgcgtc tcgtactttc gccatgcgct gcgggtgagc
      781 gcgacccgga gctggaacac gtgcctcgag cgggcgcgcg gcgaatggac gcacattctg
      841 ctcgcgaacg atgtggtgcg gcccgatttc tatacgacgc tgcgccgcac gctggaggct
      901 aaccccggcg tggaggcgtt cgcctgcaga tgcgccttga ccgatgacga gggattttgg
      961 ttgggacttt ccgagccgca ggcgaaagtt gccggctgct tgccggccga gtttccgatc
     1021 cgccagatcg ttcgccacag gctcaatctt tcaagcctcg tgatccggcg ggatttcttc
     1081 gagcaatttg gagggtttca ggagcggctg cggtactgcg ccgattggga cctgtgcaac
     1141 cggcttgccc tcaagcgaca gattttctac gacccgcatt tcctggtcag cgttcgcgaa
     1201 catgaagggg agggccgctt cctctacatt ccatccgggg agaatgtcgc cgaggcgaga
     1261 gaatgtctgc gcatctacgc ggcgctgttt ccgggcaatc ggcggcggac gatctatagc
     1321 gacggaatga cgtacgccgc tctcgatgcc ctacgccggt gcaggcatta ccgtgcggac
     1381 gggaaggcga agggcgcacg gcgtcaactg ctcgaggccg gtatctgcat gttgggaata
     1441 gcgcggtcgt acgcgatgat gccccttcgg cgcttcgcaa tccgtgagac cagcacggtt
     1501 ggtcgttaaa cggcctctct caaacgcgac catatccgcg ccgccgtcaa gtcgttctcg
     1561 ctgcccgcgc gcgtccggtt ggccggacat cgcggctcct gggcgcatcg gagctgctga
     1621 ctacgccgcc gagacggcat gagcattggc ggcggagcct ttccgctgcc gaaaccatat
     1681 gatggttgcc gcgagaccat ccggcagcga gacgcgcggc tcgaacccgt agcggcgcgc
     1741 ggtcgaggta tcggcgagcc gcaccatctc ccgaccgcgc gcgccgtcgc cgatgtgcag
     1801 gagatgctcc ttgccgatct gccgcgcgat ctcgcgggcg acatcgccga tcgtggcgcc
     1861 gtccccgctg ccgagatcga tcgtccggcg gggcggatct tcggcagcgg cgatgccgag
     1921 catcccgtcg atcacgtcat cgacgtagat ccaatcgacg aggcgcgagc cgctgccgat
     1981 cgcggcgggc cggtcatgca gaagcgagag aatcgtgctc ggcacgatct tgaactcctt
     2041 ctgcccggga ccgtaggtca tgaacggccg gacgatccgc acgtcgaggc cgtactggct
     2101 ccggaacatg cggccgtatc cgcacgtcgc ccatttcgcc gccgcatacg gcgacagcgg
     2161 cgtcggctcc tcgtcggcgc ccggatttgg ctcctccagc gaccccgtca tgaggaatcg
     2221 cgaaac
//
LOCUS       DASUJG010000063         3213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_262550, whole genome shotgun sequence.
ACCESSION   DASUJG010000063 DASUJG010000000
VERSION     DASUJG010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262550"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VFX38_01240"
     CDS             <1..253
                     /locus_tag="VFX38_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313520.1"
                     /translation="DGNLNAGEQVHSPVYQAPAGVENATLTGPSGFQLYAVFQNAQGR
                     KMYRIPGNEVHRRAPAGYFTWVAKAGDTGGVYTLCVLHP"
     gene            complement(447..2504)
                     /locus_tag="VFX38_01245"
     CDS             complement(447..2504)
                     /locus_tag="VFX38_01245"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX5313521.1"
                     /translation="MTVRRPQKKASAPAPPRGLAFRDFDPQAAATEFLRSIEAARETV
                     DEAAAEGGEGLIASLEIVGEDLDRRFAPLRHLNAVASTPAVRSAYETALAAFTGYETW
                     LGQHQRLYRRYQSLARAADLAERPPGERALIAHALTGFRLSGADLPEQERSAVRRLIG
                     ELSELENRFQECLREASEAWREPIAEEAELAGIPEPVVALLREHAAQENRDGWLLTLD
                     PGIVDAVLIHAENRALRRRVYDAWVSRASQGDHDNAPVIEAILARRRRLARALGYANF
                     ADYALVERMAKNAEEVSAFLRDLAGRARARAQQELAELSRFAAEQGLEGALAPWDISY
                     YGERLRKNRYGLDDESLRRFFPLERVLAGLRALCRELYGLRFRSIKDASTWHPEVRVW
                     TLTRGGETVGRLYLDLYARPGKRGGAWMDEGGQRLRLAGLTRRPMAFLNADFLHPARG
                     RPSLLTHEDVVTLCHELGHCLHHLLTTVDYPSVAGISGVEWDAVEFPSQLHEEFAWHP
                     AGLAQLAAEADSGAAMPGELVQALGASRRFQGALHLLRQIEFALFDLEVHRLAKAAPS
                     IAELRCVLADVQCEVRVTPVSPRDRSECNFAHIFGGGYAAGYYSYLWAEVMARDGFAA
                     FVDGAELRRDAGERLAGTVLAAGGSRPARELYRAFRGRDPQSAPLLAAYGIAWQASPP
                     VPP"
     gene            complement(2501..2848)
                     /locus_tag="VFX38_01250"
     CDS             complement(2501..2848)
                     /locus_tag="VFX38_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015602258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX5313522.1"
                     /translation="MIHKGSCHCGKVACEADCEIGEVVSCNCSICRRKGHLLLFVPLD
                     KFKLTTPRDDLSDYSFGPQRIHHHFCRACGCSPFALGSDPKGNEMAAINVRCLEDVDL
                     DALTIRHFDGRSL"
     gene            complement(2931..>3213)
                     /locus_tag="VFX38_01255"
     CDS             complement(2931..>3213)
                     /locus_tag="VFX38_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX5313523.1"
                     /translation="HIWGNGYAAGYYAYMWTQMLCDDTYQWFLDHGGLTRANGQRYRD
                     MILSRGNSADPAKMFRAFYGSAPKIGPMLKYRGIAGTAAPAAASTGSGQ"
BASE COUNT          537 a         1046 c         1108 g          522 t
ORIGIN      
        1 cgacggcaac ctgaacgccg gcgaacaggt gcattcgccg gtttaccagg caccggcggg
       61 cgtggaaaat gcgacactca ccggaccgtc gggcttccag ctctatgcgg tgttccagaa
      121 cgcccagggg cgcaagatgt atcgcatccc cggcaacgag gtgcatcgtc gggcgcctgc
      181 cggctacttc acgtgggtgg caaaagcggg cgacactggc ggtgtctata cactctgcgt
      241 gctgcacccg tgacaaaagt ttgctgattc cataacgggt cggttgactg ggccccgcga
      301 cagtgctgcc gcggggcttt ttctttcgcc cttgttcgcc cccgtctctc gcgaaggagg
      361 aagcacgagt catgcccttt ccttcgcgaa ggtggacgca ggattattgg tccttaaatt
      421 ttgcgaagcg aaaacccttc aacgccttac ggcgggacgg gaggggacgc ctgccaggcg
      481 atgccatagg cggcgagaag cggcgcggac tgcggatcgc gcccgcgaaa ggcgcggtag
      541 agttcacggg cggggcggct tccgccggcc gcgagcaccg tgcctgcaag gcgctcgccg
      601 gcgtcgcggc gcaactcggc gccgtcgaca aaggccgcga aaccgtcgcg agccatcacc
      661 tccgcccaga gataactgta ataaccggca gcgtagccgc cgccgaagat gtgcgcgaag
      721 ttgcattcgc tgcgatcccg cggcgagacc ggagtaaccc gcacttcgca ttgcacgtcg
      781 gcgagcacgc agcgcagctc cgcgatcgat ggtgctgcct ttgcgaggcg atggacctcg
      841 agatcgaaga gcgcgaattc gatttggcgc aacagatgca aggcgccctg gaagcggcgc
      901 gaggcgccga gtgcctgcac gagttctccc ggcatggcgg cgccgctgtc ggcctcggcg
      961 gcaagctgcg cgagccccgc gggatgccag gcgaattcct cgtgcagttg actcggaaat
     1021 tcgacggcat cccactcgac gccggaaatg cccgcaaccg acggatagtc caccgtcgtc
     1081 agcagatggt gaagacagtg gccgagttca tggcagagcg tgacgacatc ttcgtgcgtc
     1141 agcaaggagg gtcggccgcg cgccggatgc aggaagtccg cgttcaggaa tgccatgggc
     1201 cggcgcgtga gccccgcgag ccgcaggcgc tggccgcctt cgtccatcca agcgccgccg
     1261 cgtttgccgg ggcgtgcata aagatcgaga tacaggcgcc cgacggtttc gccgccgcgc
     1321 gtgagcgtcc agacgcgaac ctccggatgc caggtggagg cgtccttgat gctgcggaaa
     1381 cgcagtccgt aaagttcgcg gcaaagcgcg cgcagtccgg cgagcacccg ctccagcggg
     1441 aagaagcggc gcagactctc gtcgtcgagg ccgtagcgat tcttgcgcag ccgctcgcca
     1501 tagtaggaga tgtcccaggg tgcgagcgcg ccctcgaggc cctgctcggc ggcgaagcgc
     1561 gagagttccg cgagttcctg ctgggcgcgg gcgcgcgcgc ggccggcgag atcccgaagg
     1621 aatgcggaca cttcctccgc gttttttgcc atgcgctcga cgagcgcata gtcggcgaag
     1681 ttcgcgtagc cgagtgcgcg cgcgagtcgg cgccggcgcg cgagaatcgc ctcgatgacg
     1741 ggcgcgttgt catgatcgcc ctgggatgcg cggctgaccc aggcgtcgta gactcgccgg
     1801 cggagcgccc gattttccgc gtggatgagc accgcatcca cgatgcccgg atcgagcgtg
     1861 agcagccagc catcgcggtt ctcctgcgcg gcatgctccc ggagcagagc caccacaggc
     1921 tcgggaatac cggcgagttc ggcctcctcc gcgatcggct cgcgccaggc ttcgctcgcc
     1981 tcccgcaaac attcctggaa gcggttttcg agttcggaca gttcgccgat cagccggcgc
     2041 acggcgctgc gctcctgctc cggcagatcg gcgcccgaga ggcggaagcc cgtgagcgcg
     2101 tgcgcgatca gcgcccgttc tcccggcggg cgctcggcca aatccgcggc ccgcgcgagc
     2161 gactggtaac gccggtagag gcgctggtgc tgcccgagcc aggtctcgta cccggtgaag
     2221 gcggcgagcg ccgtttcgta ggcgcttcgc acggccggcg tcgatgcgac cgcattcaga
     2281 tgccgaagcg gcgcgaagcg gcgatcgaga tcctcgccga cgatctccag cgatgcgatc
     2341 aggccctcgc cgccttctgc ggcggcctcg tccaccgttt cgcgtgccgc ctcgatgctg
     2401 cgcagaaact cggtggctgc cgcctgcgga tcgaagtcgc gaaacgccag cccacgcggc
     2461 ggggccggtg ccgacgcctt cttttgcggg cggcgaaccg tcaaaggctg cggccgtcga
     2521 aatgccggat cgtgagcgca tcgaggtcca cgtcctcgag gcagcgcacg ttgattgccg
     2581 ccatctcgtt gcctttgggg tcgctgccga gcgcgaacgg cgagcagccg caggcgcgac
     2641 agaagtgatg atggatgcgc tgcgggccga agctgtaatc ggaaaggtcg tcgcgcggcg
     2701 tcgtgagctt gaacttgtcg agcggcacga agaggagcaa atggcccttg cgtcggcaga
     2761 tggagcagtt gcaggagacc acctcgccga tctcgcagtc cgcctcgcag gcgaccttgc
     2821 cgcagtggca gcttcccttg tgaatcatgg gttccctccc gtcgtgcgta caaggaaggg
     2881 gccggcaatg cctgccggcc cccgatgaaa acgctccgct cagcgtcggt tcattgtccg
     2941 gagccggtcg aggccgcggc cggcgcggcg gtcccggcaa tgccgcggta tttcagcatc
     3001 gggccgatct tcggcgcact gccgtagaac gcgcggaaca tcttcgccgg atctgcgctg
     3061 ttgccgcgcg agaggatcat gtcgcggtag cgctgtccgt tcgcgcgcgt gagtccgccg
     3121 tgatcgagga accactggta ggtatcgtcg cagagcatct gcgtccacat gtaggcgtag
     3181 tagcccgcgg cgtagccgtt gccccagatg tgc
//
LOCUS       DASUJG010000064         2628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_996751, whole genome shotgun sequence.
ACCESSION   DASUJG010000064 DASUJG010000000
VERSION     DASUJG010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996751"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..2071)
                     /gene="shc"
                     /locus_tag="VFX38_01260"
     CDS             complement(161..2071)
                     /gene="shc"
                     /locus_tag="VFX38_01260"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HEX5313524.1"
                     /translation="MRPNGGAAGIAAAGSKLESAVGRARDALLALRRDDGHWCFEFEA
                     DCTIPAEYVLMMHFMDEIDSGLETRIARYLRAKQQGDGGWPLYPGGAMNVSCTVKCYY
                     ALKLVGDDPAAEHMRRAREAVLAAGGAARANVFTRIMLAQFRQIPWRGVPFIPVELML
                     APRWMFFHIYKVSYWSRTVMVPLFILVSLRREARNPRGVGVGELFTTAPERERQWFKV
                     RSTLNRVFLVAERTARQLERFIPWGMRWAAIRRAERWMIARLNGEGGLGAIFPAMVNA
                     YEALDALGYGAAHPLRKTARRALELLLMERGDASYCQPCVSPVWDTGLAVLALQEAGA
                     SRNEVRLALDWLAARQLLDTPADWKIYRPKLAGGGWAFQYRNDYYPDLDDTSVIAWAM
                     HLHDAQAFAPQIERAAGWLAGMQSKNGGFASFDADNTSYYLNEIPFADHGALLDPPTE
                     DVTARCVALFSLLGERYRRPRERALAYLTRKQRPNGAWWGRWGCNYLYGTWSVLAALD
                     LAGIAPEDARLRRAAAWLKSVQHEDGGFGESNDSYEDAALAGRGAATPEQTAWALLGL
                     MAAGEAASEAVRRGIDWLLAAQHEDGLWHTDRFNAPGFPRVFYLRYHGYSAYFPLWAL
                     ARYHALAGVGDV"
     gene            complement(2068..>2628)
                     /locus_tag="VFX38_01265"
     CDS             complement(2068..>2628)
                     /locus_tag="VFX38_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HEX5313525.1"
                     /translation="HLPELAPRRAELLRLAVSFGQCLQMTNILKDFWQDRANGACWLP
                     RDVFLAEGVDLVQVRPGDEGFARGYRRLIGLARAHGENALAYTLAIPPQQTGIRNFCL
                     WALFMAGLTQRKLLAHPDFASGKEVKITRASVRRTVAWCRLASRSDVLLRGSFALLVH
                     RLPEPLSGDVHSSAPSRTAAETGPAR"
BASE COUNT          470 a          879 c          896 g          383 t
ORIGIN      
        1 agcagcaact cggcctcgcg gcgcgccgac tcggcgccga ttccactgac gcgcaggcgc
       61 cagccgcggc ccgcgagaag ctgcgcgctc gcggcctccg ccgcgagcgc aacgacgacg
      121 ccggcgagac caccgcgaat ccgatcgtcg gccgtcgtga tcaaacgtct ccgacgccgg
      181 ccaaggcgtg ataacgagca agcgcccaga gcggaaagta ggcgctgtag ccgtgatatc
      241 gcagataaaa cacccgcgga aagccggggg cattgaaccg atcggtgtgc cagagcccgt
      301 cttcgtgctg cgcagcgagc agccagtcga ttccgcggcg caccgcctcg ctcgccgcct
      361 cgcccgcggc catgaggccg agaagcgccc aggcggtctg ctcgggcgtc gccgcaccgc
      421 gtccggcgag cgccgcgtct tcatagctgt cgttgctttc gccgaagccg ccgtcttcgt
      481 gctgcacgga tttcaaccag gccgcggcgc ggcgcaggcg cgcatcctcc ggcgcaatgc
      541 ccgcgaggtc gagcgccgca agcaccgacc aggtgccgta gaggtaattg cagccccagc
      601 gtccccacca ggcgccgttc ggacgctgtt tgcgggtgag gtacgcgagc gcgcgttcgc
      661 gcgggcggcg ataccgctcc ccgagaagcg aaaagagtgc gacgcaacgc gccgtcacgt
      721 cttcggtggg cgggtcgagc aacgcgccgt gatcggcgaa gggaatttcg ttgagatagt
      781 agctggtgtt gtcggcgtcg aaggaggcga atccgccgtt cttcgactgc atgcctgcga
      841 gccagccggc ggcacgttcg atttgcggcg cgaaggcttg cgcgtcgtga aggtgcatgg
      901 cccaggcgat gacgctggtg tcgtcgaggt ccggataata gtcgttgcgg tactgaaaag
      961 cccagccgcc accggcgagc ttcgggcgat agattttcca gtccgcgggc gtgtcgagaa
     1021 gctggcgcgc ggcgagccaa tcgagcgcga ggcgcacttc gtttcgcgac gcacccgcct
     1081 cctgcagcgc aaggacggcg agccccgtgt cccagaccgg cgagacgcag ggctggcagt
     1141 agctcgcatc gccgcgctcc ataagcagca gttcgagcgc acgcctggct gtttttcgca
     1201 gcggatgcgc ggctccgtag ccgagggcat cgagcgcctc gtaggcgttg accatcgccg
     1261 gaaagatcgc accgagtccg ccttcgccgt tgaggcgcgc gatcatccag cgctccgcgc
     1321 gccggatcgc ggcccagcgc atcccccagg gaatgaagcg ctcgagctgg cgggccgtac
     1381 gttcggcgac gagaaacacg cggttgagcg tcgagcgcac cttgaaccat tgacgctcgc
     1441 gctcgggcgc ggtcgtgaac aattcgccga cgcccacgcc gcgaggattg cgggcctcgc
     1501 ggcgcagact cacgaggatg aaaagcggca ccatcacggt gcgcgaccaa taggaaacct
     1561 tgtagatgtg aaagaacatc cagcgcggcg caagcatcaa ttccaccggg atgaacggca
     1621 cgccgcgcca gggaatctgg cgaaactgcg cgagcatgat gcgcgtgaag acgttcgccc
     1681 gcgccgcgcc tcccgcagcg agcaccgctt cgcgcgcgcg gcgcatgtgt tccgccgcgg
     1741 gatcgtcgcc gacgagtttc agcgcgtagt aacacttcac cgtgcaggag acgttcatcg
     1801 caccgccggg atagagcggc cagccgccgt cgccctgctg tttggcgcga aggtagcgtg
     1861 cgatgcgcgt ctcgagtccg gagtcgatct cgtccatgaa gtgcatcatc agcacgtact
     1921 cggccggaat cgtgcagtcg gcctcgaact cgaaacacca atggccatcg tcccgccgca
     1981 aggcgaggag cgcatcgcgc gcgcggccga cggccgactc caatttcgag ccggcggcgg
     2041 caatcccggc ggcgccgccg ttgggtctca tcgcgccggc cccgtttccg cggcggtccg
     2101 cgacggcgcc gaagaatgga cgtcgcccga cagaggctcg ggcaaacgat ggacgaggag
     2161 tgcgaaactt ccccggagaa gcacatcgga gcgcgacgcc agacggcacc aggcgacggt
     2221 gcgccgcacg ctcgcgcgag tgatcttcac ttccttgccg ctcgcaaaat ccggatgggc
     2281 gagaagcttc cgctgcgtga gcccggccat gaagagcgcc cacagacaga agtttcggat
     2341 tcccgtctgc tgcgggggaa tcgcgagcgt gtaggcgagc gcgttttcgc cgtgcgcgcg
     2401 tgcgagcccg atcagacgcc ggtaaccgcg cgcaaagcct tcgtctcccg ggcgcacctg
     2461 cacgagatcg acgccctcgg cgaggaacac atcgcgcggc agccagcagg caccgttggc
     2521 gcgatcctgc cagaaatcct tgaggatgtt ggtcatctgc aggcactgcc cgaaggaaac
     2581 cgcgagtcga agcagttccg cgcggcgcgg agcgagttcg ggaagatg
//
LOCUS       DASUJG010000065         2003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1609056, whole genome shotgun sequence.
ACCESSION   DASUJG010000065 DASUJG010000000
VERSION     DASUJG010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609056"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(291..668)
                     /locus_tag="VFX38_01270"
     CDS             complement(291..668)
                     /locus_tag="VFX38_01270"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX5313526.1"
                     /translation="MTEQRQDTRHRSLLRGIVYFDGRPFAHECLVRDISEKGARIAFP
                     DPPPAHADRLELQVPIKSLRHQCRVAWRGDAEVGVSFTDVAATEMSPQAMAERMARLE
                     AELESLKQIVRALRGEMKADHAA"
     gene            complement(773..1720)
                     /gene="metA"
                     /locus_tag="VFX38_01275"
     CDS             complement(773..1720)
                     /gene="metA"
                     /locus_tag="VFX38_01275"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010658688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEX5313527.1"
                     /translation="MPIKIPDNLPARHVLETEGVMVMAETDAIRQDIRPMRIGLLNLM
                     PNKVKTETQFARLLAATPLQVELTLVKMTNHVPRNTPTDHIVSFYRDWEDVRAEKFDG
                     FIVTGAPVELLPFEEVTYWDELRRVFDWTQTNVHGTMTICWGAQAAVHHFHGMPKYPL
                     AQKMFGVYRHRIFEPASPYLRGFSDDFSIPVSRWTEVRREDIPSGRRLSVLMDSDEAG
                     LCLLDDPAHRALHMFNHIEYDSSSLADEFFRDRAARQPIAMPKNYFPGDNADAQPLNR
                     WRSHAHLLFGNWINQLYQTTPVDLRAVGTRGEESMVNAR"
     regulatory      complement(1757..1862)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM-I-IV-variant riboswitch; SAM riboswitches
                     modulate the expression of downstream genes in response to
                     levels of S-adenosylmethionine; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
BASE COUNT          363 a          604 c          670 g          366 t
ORIGIN      
        1 gaagtcgatc gcggcgccga cgaaggcgat cagcggcaac acggcgaggg aaaagatgac
       61 ggcgacattg cctcgccggt cctttcggaa cgtgagggcc gagcggcgaa tactctcgag
      121 aatgcgtcgc accgcgctgc tccatcgcga gatttgcaat ggaaacttac atgcggaact
      181 cgtaaaattg agttgcagac gcgagtcagc tcgccctacg gcggaggtct gtgatttgat
      241 ggttgcccgc gcgttaacgc ggcagttcgc ggacaagagg cggttcccac tcaggcggcg
      301 tgatccgcct tcatctctcc gcgcagcgcg cgcacgatct gcttcagcga ttccagttcg
      361 gcttcgagcc gcgccatgcg ctcggccatc gcctgcggac tcatttcggt cgcggcaacg
      421 tcggtgaaag acacaccgac ttcggcatcg ccgcgccacg cgacgcgaca ttgatgccgc
      481 aaggatttga tggggacctg caattcgagc cggtcggcgt gagccggcgg cgggtccggg
      541 aacgcgatcc gtgcgccctt ctcggagatg tcgcgcacga gacactcgtg ggcgaacggc
      601 cggccgtcga aatagacgat gccgcgcagc aggcttcggt gtctcgtatc ctggcgctgc
      661 tcggtcatgg cttgctcgcc gtttcgacga tgcccgaacc tgccccggat cgtttgaaga
      721 aaagctgaaa caatgcggtc aacggaacac taaacgatgc gccgcaagcg cgctaacgcg
      781 cgttgaccat gctctcctcg cctcgggtgc cgaccgcgcg cagatcgacc ggagtggtct
      841 ggtagagctg gttgatccag ttgccgaaca aaaggtgcgc gtggctgcgc cagcggttga
      901 gcggctgcgc gtccgcattg tcgcccggga aatagttttt cggcattgca atcggctgcc
      961 gcgcggcccg atcgcggaaa aattcatcgg cgagcgaact gctgtcgtac tcgatatgat
     1021 tgaacatgtg cagcgcgcga tgtgccggat cgtcgagcag gcagagaccc gcctcgtccg
     1081 agtccatcag cacggacaga cgccggccgg atggaatgtc ctcacgccgc acctcggtcc
     1141 agcgcgaaac cggaatcgag aagtcgtcgg agaagccgcg caggtagggc gaggcgggct
     1201 cgaagatgcg atggcgatag acgccgaaca tcttctgcgc gagcgggtac ttgggcatgc
     1261 cgtggaagtg atgcaccgcc gcctgcgcgc cccagcagat ggtcatggtc ccgtgcacgt
     1321 tggtctgcgt ccagtcgaag acgcggcgca gttcgtccca gtaggtcacc tcctcgaacg
     1381 gcagcaactc gaccggcgcg ccggtgacga tgaagccgtc gaacttctcg gccctgacgt
     1441 cctcccagtc gcgatagaag gagacgatgt ggtcggtcgg cgtattgcgc gggacgtggt
     1501 tcgtcatctt caccagagtg agttcgacct gcagcggggt cgcggcgagc aggcgcgcga
     1561 actgcgtctc ggtcttcacc ttgttcggca tcaggttgag cagcccgatg cgcatcggcc
     1621 ggatgtcctg ccggatggcg tcggtctcgg ccatgaccat gacgccctcg gtttcgagga
     1681 catgacgggc gggcaggtta tcgggaatct tgatcggcat gactcgggtc tcgtgtgcga
     1741 gcgagaccga gtgtgactga gcccgtttgg ttgccggttc ggccacatcc tccctcgagg
     1801 gagcgccacc ttgctcggga agcaggttgg cgtcgggcat cgcccaactc tggatgtcga
     1861 agaagctttt agggccgggc ggcgggtttg gcaacaggta ggcgggacat gggaccccgc
     1921 gtggcaaagg ccgcctgaac tgctatatca tcgtcatgat cacggccgcc gcattgtgcc
     1981 tgcccgagcg tctcgccgcc gag
//
LOCUS       DASUJG010000066         2457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1364380, whole genome shotgun sequence.
ACCESSION   DASUJG010000066 DASUJG010000000
VERSION     DASUJG010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364380"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VFX38_01280"
     CDS             complement(<1..263)
                     /locus_tag="VFX38_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019519212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEX5313528.1"
                     /translation="MAIRKAKPTSPGRRFVLEPTREGLHKGRPEKSLVETQTRSGGRN
                     NQGRMTVRHQGGGHKRLYRRVDFRRNKDNVPARVERIEYDPNR"
     gene            complement(276..575)
                     /gene="rplW"
                     /locus_tag="VFX38_01285"
     CDS             complement(276..575)
                     /gene="rplW"
                     /locus_tag="VFX38_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEX5313529.1"
                     /translation="MNADRLAEIIRAPQVSEKATRLAETHRQIVFRVRPEASKPQIKR
                     AVEAMFEVKVAKVTTLRRQGKSSGRATGGGGRHSDWKKAYVTLQPGFDIDFAGTE"
     gene            complement(572..1180)
                     /gene="rplD"
                     /locus_tag="VFX38_01290"
     CDS             complement(572..1180)
                     /gene="rplD"
                     /locus_tag="VFX38_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX5313530.1"
                     /translation="MELQIQGNGQKPIEVSEAAFGQAYNEPLIHQAVTAYLAGARAGS
                     KAQKTRAAVRGGGRKPWRQKGTGRARAGSIRSPLWRGGGKIHAAAPRDHRVKLNRRMY
                     RGAMRSLLSELARRDRLVILEDFAIERPKTAELAGRLREYGLVDVLIVTAQTSEALLL
                     ASRNLKAVDVRAVRELDPVSLIAFDKVAVTVPALRELEKWLG"
     gene            complement(1195..1833)
                     /gene="rplC"
                     /locus_tag="VFX38_01295"
     CDS             complement(1195..1833)
                     /gene="rplC"
                     /locus_tag="VFX38_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003103877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX5313531.1"
                     /translation="MSLGVIGRKCGMTRIFTEDGGAVPVTVIEVLPNRVAGLRADDGG
                     RAVQLAAGTRRAGRINKPTAGNFAKAGVGASRVLREFRLAGDEGAELEAGKEVGVGIF
                     TAGQKVDVTGRSLGKGYAGTVKRHHFRTQDASHGNSVSHRVPGSIGQNQSPGRVFKGK
                     RMSGHLGAARRTAQNLEVVRVDETRNLLLLKGAVPGPAGGDLLVRPAVKARG"
     gene            complement(1928..2239)
                     /gene="rpsJ"
                     /locus_tag="VFX38_01300"
     CDS             complement(1928..2239)
                     /gene="rpsJ"
                     /locus_tag="VFX38_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEX5313532.1"
                     /translation="MANERIRIRLKAFDHRLVDNSAREIVETARRTGARVKGPIPLPV
                     RIERYTLLASPHKDKDARDQYELRTHKRLLDIVEPTDKTVDALMKLELAAGVDVQIRL
                     N"
     gene            complement(2373..>2457)
                     /locus_tag="VFX38_01305"
     CDS             complement(2373..>2457)
                     /locus_tag="VFX38_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003806883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX5313533.1"
                     /translation="AMEEGVRFAIREGGHTVGAGVVSKVIK"
BASE COUNT          361 a          881 c          750 g          465 t
ORIGIN      
        1 ctgcggttcg gatcgtactc gatgcgctcg acccgcgccg gcacgttgtc cttgttgcgg
       61 cggaagtcca cgcgccggta caggcgcttg tgcccgccgc cctggtgacg cacggtcatg
      121 cggccctgat tgttgcgccc gccgctgcgc gtctgcgttt ccaccaggga cttctcgggg
      181 cggcccttgt gcagcccctc gcgcgtcggc tcgagcacga agcggcggcc cggggaagtc
      241 ggcttggcct tgcgaattgc catgatccag ctgtcctatt ccgttccggc gaagtcgatg
      301 tcgaagccgg gttgcagggt cacgtaggcc ttcttccaat cggagtggcg cccaccgccg
      361 cccgtcgcgc gtccgctcga tttcccctgc cggcgcaacg tcgtcacctt cgcgaccttg
      421 acctcgaaca tcgcttccac cgcgcgtttg atttggggct tgctcgcttc cgggcgcacc
      481 cggaagacga tctgccggtg agtctcggcg agccgcgtcg ccttctccga aacctgtggc
      541 gcgcggatga tctcggcgag ccggtcagcg ttcaacccag ccatttttcg agctcccgca
      601 gggctggcac cgtgaccgcg accttgtcga aggcgatgag gctcacggga tcgagctccc
      661 tcacggcccg cacgtccact gccttgaggt tgcgcgaggc gagcagcaag gcctcgctcg
      721 tctgcgcggt gacgatcagc acatcgacca ggccgtactc gcgcaaccgg cctgcgagtt
      781 ccgccgtctt cggccgctcg atcgcgaaat cttccaggat caccagccga tcccgccgcg
      841 cgagctccga aaggagactg cgcatcgcac cgcgatacat ccgtcgattg agcttcacgc
      901 ggtgatcgcg cggcgccgcc gcatggatct tgccgccgcc gcgccacagc gggctgcgga
      961 tgctgccggc gcgggcacgt cccgtaccct tctggcgcca gggcttcctg ccacccccgc
     1021 gcacggcggc acgggtcttc tgcgccttgc tgcccgcacg ggcgccggcg aggtaggccg
     1081 tcaccgcctg atgaatcagg ggttcgttgt aggcttgccc gaacgcagcc tccgagacct
     1141 cgatcggttt ttgcccgttt ccttgaatct gaagttccat cgcgtcgaat ccgttcagcc
     1201 gcgggccttg acggcggggc ggacgagaag atctccgccg gccgggccgg gcacggctcc
     1261 cttgagaagc agcaggttgc gggtctcgtc cacccgcacg acctcgaggt tctgggcagt
     1321 gcggcgtgcg gctccaaggt gcccggacat ccgcttgccc ttgaatacgc gccccggcga
     1381 ctggttctga ccgatcgagc ccggaacgcg atgggaaaca gaattgccgt gactggcatc
     1441 ctgcgtgcgg aaatgatggc gcttgaccgt gccggcatag cccttgccga gcgaacggcc
     1501 cgtcacatcc accttctgcc ccgcggtgaa gatcccgacg cccacttcct tgcctgcctc
     1561 cagctccgcg ccctcgtccc cggcaagccg aaattcgcga agcacccggc tggcccccac
     1621 cccggccttg gcaaaatttc cggccgtcgg cttgttgatg cgcccggccc gccgggtccc
     1681 ggccgcgagc tgcaccgccc ggccgccgtc atccgcccgc aggcccgcga cccgattcgg
     1741 gagcacctcg atcacggtta cgggtacggc gccgccgtct tccgtgaata tacgggtcat
     1801 gccgcatttg cggccgataa cgcccaggct catggctcca tgcctcgcga caacggcgac
     1861 gggatgtcgg cgtcggaatt tcccgcctcg aatcgaggcg agctggttat tatagcagcg
     1921 aaattcctca gttcaagcgg atttgcacgt cgacgcccgc cgcaagttcc agcttcatga
     1981 gcgcatccac ggtcttgtcg gtcggttcga cgatgtcgag caggcgcttg tgcgttcgca
     2041 actcatactg gtcccgcgca tccttgtcct tgtgcggcga ggcgaggagc gtgtagcgct
     2101 cgattcgcac cggcagcgga atcggcccct tgacgcgggc gccggtgcgt cgcgcggtct
     2161 ccacgatttc gcgcgccgaa ttgtccacca ggcgatggtc aaaggccttc agccggatgc
     2221 ggattctttc attcgccatt gctcactccg ttgaaaagcg ccagtcgctc gctgtcagca
     2281 gatcaggggt acgttctgcc cactttaaac gacctgcgca caaactccac ttgttcactc
     2341 acttgcaggc cgcgcagcgg ccggctcact gcctacttga tcaccttgga gactaccccg
     2401 gctcccaccg tgtggccccc ttcgcggatc gcaaacctca ccccctcctc catcgcg
//
LOCUS       DASUJG010000067         2855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_997845, whole genome shotgun sequence.
ACCESSION   DASUJG010000067 DASUJG010000000
VERSION     DASUJG010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997845"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="VFX38_01310"
     CDS             <1..742
                     /locus_tag="VFX38_01310"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5313534.1"
                     /translation="GRRNFTVATLAISLGYLMFFGSVVIFPLWLQTQMGYTATWAGIA
                     AAPIGLLAIFVAPMLGRLMRRFDPRVLVTVSFALFAGASFWMSRFATNVDLLQLMLPR
                     LVFGIGIPLFFIPLISLSLSGLPPERIASASGLFNFMRMLAGGFGASLSITLWDQRQA
                     LHDAHLSAAVHLHDAFTAPIFARLGELGMSAKASLAELSHVVEQQSFMLATNDFFWLS
                     GWVFLALLGLVWFARKSAGRSAPVGGGE"
     gene            787..1428
                     /locus_tag="VFX38_01315"
     CDS             787..1428
                     /locus_tag="VFX38_01315"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX5313535.1"
                     /translation="MNAIDRSDIFGKRSFLRAALWLAQALIAGVYLSTGIAALVLPAA
                     QISAIVPWATHLPGAVLIFLCAAELSAGAGVLLPSVTRIAPALSVTAAVCSAALQAIA
                     IFFHALLGMPQMLPVGVIVFALSVFVAWGRSDREPIASRWQDRRMAPLDAFAGGRVHA
                     SEGRIERRRRATRRQRKSDRASFTIRRGLRATPFRRANKGKPARRDRPSSIRD"
     gene            complement(1602..1898)
                     /locus_tag="VFX38_01320"
     CDS             complement(1602..1898)
                     /locus_tag="VFX38_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX5313536.1"
                     /translation="MKPKMQPCVYLLANKPHGTLYVGVTSDLVKRIWEHKNNVVEGFT
                     QKYNVHALIWFEQHETMQSAISREKAIKKWNRAWKTKLVEKNNPEWRDLYPDLV"
     gene            complement(1956..>2855)
                     /locus_tag="VFX38_01325"
     CDS             complement(1956..>2855)
                     /locus_tag="VFX38_01325"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX5313537.1"
                     /translation="MKAAMLAHDSILVGTNDIVVAGGMESMTNAPHMVHMRAGVKYGE
                     AKLVDHMAWDGLTDPYENQAMGIFGELCADKFHFSREDQDAYAIESVKRSQRAVADGS
                     FAAEIVPVTVKTRKGEVVVDTDEEPGRVNVDKIPNLKPSFKKDGTVTAASSASISDGA
                     AALVLMRASEAKLHGVKPLARILGHSVFSQQPAWFTTSPVGAIDRLHTKLGWTEKDVD
                     LYEINEAFAVVAMAAMKELALPHEKVNVNGGACALGPPIGCSGARITVTLLYALKARG
                     LKRGIASLCIGGGEATALAVEML"
BASE COUNT          442 a          897 c          889 g          627 t
ORIGIN      
        1 cgggcggcgc aacttcacgg tggcgacgct cgcgatcagt ctcggctacc tgatgttttt
       61 cggcagtgtc gtcatcttcc cgctctggtt gcagacccag atgggctaca cggcgacctg
      121 ggccgggatc gcggccgcgc cgatcggcct gctcgcgatc ttcgtcgcgc cgatgctcgg
      181 aaggctcatg cggcgcttcg atccgcgagt gctcgtcacg gtttccttcg cgctcttcgc
      241 cggggcgtcc ttctggatgt cgcgcttcgc gaccaacgtg gatctcctgc agttgatgtt
      301 gccgcgtctc gtcttcggta tcggcatccc gctcttcttc atcccgctca tcagcttgtc
      361 gctgtcgggg ttgccgcccg agcgaatcgc gagcgcctcg ggactcttca atttcatgcg
      421 catgctcgcc ggcggcttcg gtgcttcctt gagcattacg ctttgggatc agcgtcaggc
      481 attgcacgat gcccatctgt ccgcggccgt ccatctgcac gatgccttta ccgcgccgat
      541 ctttgcaagg ctcggcgaac tcggaatgag tgcgaaggct tcgctcgccg aactttcgca
      601 cgtcgtcgag caacaatcct tcatgctcgc gaccaacgac ttcttctggc tctccggctg
      661 ggtctttctc gccctgctgg ggctggtgtg gttcgcccga aagtcggcgg gacgctccgc
      721 gccggttggc ggcggcgagt gagcgccgca tccgatgggt ccgccgaatc gaacggagaa
      781 gcgtccatga atgccattga ccgtagcgac atcttcggca aacggtcgtt tttgcgagcc
      841 gcactgtggc ttgcgcaggc gctgattgcg ggagtgtatt tgtccaccgg gatcgcggcg
      901 ctggttctcc ccgctgcgca gatttcggcg atagtgcctt gggcgacgca tttgcccggg
      961 gcagtgctga tctttctttg cgctgcggaa ctgagcgccg gtgcgggcgt gctgctgccg
     1021 tccgtgacgc ggatcgcgcc cgccctgagc gtgaccgcgg ccgtttgctc cgcggcgctc
     1081 caggccatcg caatcttctt tcacgccttg ctcggaatgc cgcagatgct tcccgtcggt
     1141 gtgatcgtgt tcgccctgtc cgtcttcgtc gcctggggaa gatcggaccg ggagcccatc
     1201 gcatcgcggt ggcaagaccg gcgcatggcg ccgctcgatg ccttcgccgg cgggcgcgtg
     1261 cacgcatccg aaggccgcat cgaaaggcgc cggcgcgcga cacggcggca aaggaagagc
     1321 gaccgtgcga gtttcactat tcgacggggt ttgcgcgcca ctccttttcg tcgcgcgaat
     1381 aagggaaagc cggcgcggcg cgatcgccca agttcgattc gggactgaag atcgacaagg
     1441 tgccgagtcc gtcgcaaatt cggacctcca agccttcacg caaccttcgc tgccgcgcgc
     1501 aacggggccg cattgctcga agtggcgcgt aagggtctgc tgactgccaa cacctccggc
     1561 aaggaagctc cgtcgtcatt cccgcgaagg cgggaatcca tctacacaag gtcggggtaa
     1621 aggtcgcgcc attcgggatt gtttttttcg acaagttttg tcttccatgc ccgattccat
     1681 ttcttgatgg ccttttctcg ggaaattgcg gactgcattg tttcgtgttg ctcgaaccaa
     1741 atcagcgcat gaacgttgta cttttgtgta aagccttcaa cgacgttgtt cttatgttcc
     1801 caaatgcgct tcaccagatc ggacgtgacg ccgacgtaca gcgttccgtg tggcttgttg
     1861 gccaacaaat aaacgcatgg ttgcatcttc ggcttcatgg attcccgcct tcgcgggaac
     1921 gacgaagaga catgggctcg ctttcgaacg tattctcaga gcatttcgac ggcgagggcg
     1981 gtcgcttcgc cgccgccgat gcagagcgag gcgatgccgc gcttgaggcc gcgggccttg
     2041 agcgcgtaga gcagcgtgac ggtgatgcgc gcgccggagc agccgatcgg aggcccgagc
     2101 gcgcaggcgc cgccgttgac gttcactttt tcgtgcggaa gcgcgagttc cttcatcgcc
     2161 gccatcgcca cgacggcgaa ggcctcgttg atctcgtaaa gatcgacgtc cttttccgtc
     2221 cagccgagtt tcgtgtgcag gcgatcgatg gcaccgaccg gcgacgtcgt gaaccacgcc
     2281 ggctgctgcg agaacaccga gtgtccgagg atgcgcgcga gcggcttcac gccgtggagt
     2341 ttcgcttccg aggcgcgcat cagcaccagc gccgccgcgc cgtcggaaat cgaggccgaa
     2401 ctcgccgccg tgacggtgcc gtccttcttg aacgatggct tgagattcgg aatcttgtcc
     2461 acgttgacgc ggccgggttc ttcgtcggta tccacgacga cttcgccctt gcgcgtcttc
     2521 accgttaccg gcacgatctc ggcagcgaaa ctgccgtcgg cgacggcgcg ttgcgagcgc
     2581 ttcaccgact cgatcgcata ggcgtcctga tcttcgcggc tgaagtggaa cttgtcggca
     2641 cagagttcgc cgaagatgcc catggcctgg ttttcgtatg gatcggtgag cccgtcccag
     2701 gccatgtgat cgacgagctt cgcctcgccg tatttgactc cggcgcgcat gtgcaccatg
     2761 tgcggtgcat tcgtcatgga ttccatccct ccggcaacca cgatgtcgtt ggtgccgacg
     2821 agaatggaat cgtgcgcgag catcgccgcc ttcat
//
LOCUS       DASUJG010000068         4530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1241798, whole genome shotgun sequence.
ACCESSION   DASUJG010000068 DASUJG010000000
VERSION     DASUJG010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241798"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..1438)
                     /gene="hemW"
                     /locus_tag="VFX38_01330"
     CDS             complement(224..1438)
                     /gene="hemW"
                     /locus_tag="VFX38_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX5313538.1"
                     /translation="MAAESSIIEAASGIRPCANVPLAIYVHVPWCVAKCPYCDFNSHV
                     LKGGVPAARYVTAVLADLDFELALLPAAARAISVFFGGGTPSLLAPEWVERLLAGIRA
                     RVPLVADAEVTLEANPGTIEHGRFAEYAAAGINRVSLGVQSFDDAKLARLGRIHSACE
                     AVIAAEELRRAGLDNFNLDLMYALPGQDAAGASDDVRKAVALAPAHISWYELTLEPGT
                     AFYRRPPKLPREEEAARIEEAGREELLAAGYERYEISAFARPGRRCRHNDNYWGYGDY
                     IGLGPGAHGKRSKGSAIVRTIRVLSPDRYLREAGSAAALSETRSIPPAERSFEFLLGA
                     LRRVKGFRWEEFEDRTGLSRRALAIRLRTASHCENLIAWRAEGPRPTARGLDFLNEVL
                     NRLLPAPNAPNS"
     gene            1419..4094
                     /gene="pepN"
                     /locus_tag="VFX38_01335"
     CDS             1419..4094
                     /gene="pepN"
                     /locus_tag="VFX38_01335"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEX5313539.1"
                     /translation="MLLSAATFAMDNSGNHKVVRLEDYTPPAWLVDALRLEFVLGDEA
                     TEVRAELDLRRNPANRAAENLLRLDGEHLELLGIAIDGEALAAGRYRLTERELTIAAP
                     PQHFTLATHVRIHPEANTELSGLYKSAGNFCTQCEAEGFRHITYFPDRPDVLARFTTR
                     IEAAKDACPVLLSNGNLVDSGTLAGGRHYAVWEDPFPKPAYLFALVAGRLACREDDFV
                     TASGRKVALRLYVESHNLERTAHAMASLKRAMRWDEENYGREYDLDVLMLVAVDDFNM
                     GAMENKGLNIFNSRYVLADPDIATDQDFAAIESVVGHEYFHNWTGDRVTLRDWFQLSL
                     KEGLTVFREQGFSADMGSAAVKRIRDVRTLRAMQFAEDAGPMAHPVRPDHYLEINNFY
                     TATVYEKGAELLRMYRTLLGVEGFRRGMDLYFERHDGQAVTTDDFRAAMSDANGVDLS
                     QFARWYEVSGTPRLEVAGEYDVADLRYTLHVRQVHADTPGQPAAGKPPLLIPLALALL
                     GPDGEEQPLRLHGETETASERVLWLRDFDERFVFEDLPEHPTPSLLRNFSAPVRLEYP
                     YSDADLAFLAARDSDPFNRWDAAQTLVSRVLMNRAGEKQHREAAPPASLVDAWRALLA
                     DGAGEPAVIAETLSLPTETELARKFERVDVEALHMACGALKSRLAAALQKELRAALER
                     NAPRGAYRRDAPEVGRRRLHGVILDYFGALDSPDESIMLKAAFDSADNMSDRLAALGA
                     LAAQESAERSRALDEFRARFDRHPLVLDKWFALQAVSPPAGALDRVRALMSDPAFNLR
                     NPNRVRSLIGSFVHNNHFGFHVAGGAAYRFAAEQVLALDALNPQVAARIVTCFSHWRR
                     YDEARRSAMRTELERIASAPRLSRDVCEIVARSLA"
     gene            4141..>4530
                     /gene="argS"
                     /locus_tag="VFX38_01340"
     CDS             4141..>4530
                     /gene="argS"
                     /locus_tag="VFX38_01340"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF015450.4"
                     /note="catalyzes a two-step reaction, first charging an
                     arginine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; class-I aminoacyl-tRNA
                     synthetase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX5313540.1"
                     /translation="MKEVVENLLARSLQALAIEPPERDSLAVERSRNPEHGDFASNAA
                     LVLAAATGERPRALAERIRAALPPSELVAKTEIAGPGFLNFFLAPAAWQAELARILAA
                     GESYGRSEAGSGRRVLLEFVSANPTGPL"
BASE COUNT          772 a         1527 c         1451 g          780 t
ORIGIN      
        1 tggatgaaac acggaagtcg gcgatccgat cggcgtcgac agcggggcgt tgtcaaattg
       61 gccaaaattt caccaccgcg gttattccct atcgatcaat ggcttatgaa aaatcgactc
      121 gggtaattcc ttcggttcgc gccggcgggc cgcggagcgc cggccgcgag gcgggcgact
      181 gtgcataacc gcctgattct gcgtgtgtcc gagtcgtcgg gactcaggaa ttcggcgcat
      241 tcggggccgg caggagtcgg ttcagaacct cattgagaaa atccaggcct cgggccgtcg
      301 ggcgcggccc ctccgctcgc cacgcaatca gattctcgca gtgcgacgcc gtccgcaatc
      361 ggatcgccaa tgccctgcgg gagagtccgg tgcgatcttc aaactcctcc cagcggaatc
      421 ctttcacccg ccgcagtgca cccagcagga attcgaagct gcgttcggcc ggcgggatcg
      481 atcgggtctc gctgagtgcg gccgcgcttc cggcctcgcg caggtagcgg tccggcgaga
      541 ggacacgaat cgtgcgaacg atcgcgcttc ccttgctgcg tttgccgtgc gcccccgggc
      601 cgagaccgat gtaatcgcca tagccccagt agttgtcgtt atgacggcag cgacgccccg
      661 gacgcgcgaa ggcggagatc tcgtaacgct cgtagcccgc cgcaagcaat tcctcccgtc
      721 ccgcctcctc gattcgggcg gcctcctcct cgcgcgggag cttcggcgga cggcgataaa
      781 aggcggtgcc cggttcgagc gtaagttcgt accaggaaat atgcgcgggc gcgagcgcga
      841 ccgccttgcg gacatcgtcg ctcgcgccgg ccgcatcctg gcccggaagt gcgtacatga
      901 gatcgagatt gaaattgtcg agaccggccc gccgcagttc ttcggcggcg atgacggcct
      961 cgcaggcgga atgaatgcgg ccgagacgtg cgagtttcgc atcgtcgaaa ctctgcacgc
     1021 cgagcgacac ccggttgatc ccggcggcgg cgtactcggc aaagcggccg tgctcgatcg
     1081 taccgggatt ggcctcgagg gtgacctcgg catcggcaac gaggggaacg cgcgcgcgaa
     1141 tgccggcgag caggcgctcg acccactccg gggcaagcag gctcggtgtg ccgcccccga
     1201 aaaacacgct gatcgcccga gccgcagccg gcagcaatgc gagttcgaag tccagatcgg
     1261 cgagcaccgc ggtcacatag cgcgcggccg gcacgccgcc cttgagtacg tgcgaattga
     1321 aatcgcaata cggacacttc gcgacgcacc agggtacgtg cacatagatc gcgagcggaa
     1381 cgttcgcgca gggcctgatg ccgctcgcgg cctctataat gctgctttcc gccgccacgt
     1441 ttgcgatgga caattccggg aaccacaagg tcgttcgtct cgaagactat accccgcccg
     1501 cctggctcgt cgacgcgctg cgcctcgagt tcgtgctggg agatgaggcg accgaggtgc
     1561 gcgccgagct cgatctgcgg cgcaatccag caaaccgcgc ggcggagaac ctcctgcgcc
     1621 tcgacggcga acatctggaa ctgctgggaa tcgcgatcga cggcgaagcg cttgccgccg
     1681 ggcgttaccg gctgacggag cgagaactca cgattgcggc gccgccccag cacttcacgc
     1741 tcgcgacgca cgtccgcatt catcccgagg cgaacaccga gctttcggga ctttacaagt
     1801 ccgcgggcaa tttctgtacc cagtgcgagg cggaaggctt ccgccacatc acgtactttc
     1861 ccgatcgtcc ggatgtcctc gcgcgcttca caacgcgcat cgaggcggcg aaggacgcct
     1921 gtccggtttt gctgtccaac ggcaacctcg tggattcggg gacgctcgcg ggcgggcggc
     1981 actacgccgt ctgggaagac ccctttccca agccggccta tctgttcgcg ctggtcgccg
     2041 ggcgcctcgc ctgccgcgag gatgacttcg tcaccgcgtc gggacggaag gtggcgctgc
     2101 gcctctacgt cgaatcccac aatctcgagc gcacggccca cgccatggcc tcgctcaagc
     2161 gcgcgatgcg ctgggacgag gaaaactacg gacgcgaata cgatctggat gtgctgatgc
     2221 tcgtcgccgt ggacgacttc aacatgggcg caatggaaaa caagggattg aacatcttca
     2281 attcccgcta cgtcctcgcc gatcccgaca tcgcgactga ccaggacttc gccgccatcg
     2341 aatcggtcgt cgggcacgag tacttccaca actggacggg cgaccgggtg acgctgcgcg
     2401 actggttcca gctttcgctc aaggaggggc tcacggtgtt tcgcgaacag ggtttctccg
     2461 cggacatggg ctcggcagcg gtcaagcgca ttcgcgacgt gcgcacgctg cgcgcgatgc
     2521 agttcgccga agacgccggc cccatggcgc accccgtacg gcccgaccac tacctcgaaa
     2581 tcaacaattt ctacaccgcg acggtatacg agaagggcgc cgaattgctg cgcatgtacc
     2641 gcacgctgct cggagtcgag ggttttcgcc gcggcatgga tctttatttc gagcgccacg
     2701 acgggcaggc ggtcacgacc gatgattttc gcgccgcgat gagcgacgcc aacggcgtgg
     2761 acctttccca attcgcgcgc tggtacgagg tttccggtac gccgcggctc gaagtcgcgg
     2821 gagagtatga cgtcgccgat ctccgctaca cgctccacgt tcgtcaggtg catgccgaca
     2881 cgcccggaca accggctgcg ggaaagccgc cgctcctcat cccgctcgcc ctcgccctcc
     2941 tcggtccgga cggggaggag cagcccctgc gcttgcacgg cgagacggaa acggcgagcg
     3001 aacgcgtgct gtggttgcgg gacttcgacg agcgcttcgt gttcgaggac ctgccggagc
     3061 atccgactcc atcgctgctt cggaatttct ccgcgccggt acggctcgaa tatccgtact
     3121 cggatgccga tctcgctttt ctcgccgccc gcgattcgga tccgttcaac cgttgggatg
     3181 ccgcgcagac actcgtctcg cgggtcctga tgaaccgcgc cggagagaaa caacatcgcg
     3241 aagccgcacc gccggcgtct ctcgtggacg cctggcgcgc gcttcttgcc gacggtgcgg
     3301 gcgagccggc cgtgatcgcg gagaccttga gccttcccac ggaaacggaa cttgcgcgca
     3361 aattcgagcg cgtcgatgtc gaagcgctcc acatggcctg cggcgcgttg aagtcgcggc
     3421 tcgccgcggc tctgcaaaag gagctgcggg ccgcgctcga gcgcaacgcc ccgcgagggg
     3481 cgtaccgccg ggatgccccg gaggtcggcc ggcgtcggct ccacggcgtg attctcgact
     3541 atttcggcgc gctcgactcg cccgatgaga gcatcatgtt gaaggcggcg ttcgattctg
     3601 cggacaacat gagcgatcgg ctcgccgcgc tcggcgcgct cgcggcgcag gaatcggcgg
     3661 agcgctcgcg ggcgctcgac gaattccgcg cgcgcttcga ccgccatccg ctggtccttg
     3721 acaaatggtt tgctctccag gccgtctcgc cgcccgccgg cgcgctcgat cgtgtgcgcg
     3781 cgctgatgag cgatcccgcg ttcaacctgc gcaatccgaa tcgggtgcgt tcgctgatcg
     3841 gaagcttcgt gcacaacaat cacttcggct tccacgtcgc tggcggcgcc gcctaccgct
     3901 ttgccgccga gcaggtgctc gcgctcgacg cgctcaaccc gcaggtcgcg gcccggatcg
     3961 tcacctgctt cagccattgg cgtcgctacg acgaagcgcg ccgttcggcg atgcgcaccg
     4021 aactcgaacg aatcgcttcg gcgccgcggc tttcgcgcga cgtttgcgaa atcgtcgcgc
     4081 gctccctcgc ctgagtacga ggttccctac aatgatgccg agccaaactc gttccgcgcc
     4141 gtgaaggaag tcgtagaaaa tctgctcgcg cgatccctgc aggcgctcgc aatcgagcca
     4201 ccggagcgcg actcccttgc cgtcgagcgt tcgcgcaacc ccgagcacgg agatttcgcg
     4261 agcaatgccg cgctcgttct tgcggccgcg acgggtgagc gcccgcgtgc acttgcggaa
     4321 aggattcgcg cggccctccc cccctccgaa ctcgtcgcca aaacggaaat cgcgggaccg
     4381 ggattcctga acttctttct cgcgcctgcc gcctggcagg cggaactcgc gcgcatcctc
     4441 gccgcgggcg agagttacgg caggagcgag gccggcagtg gacggcgcgt gctgctcgaa
     4501 ttcgtctcgg cgaatccgac cggtccgctt
//
LOCUS       DASUJG010000069         4274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1609690, whole genome shotgun sequence.
ACCESSION   DASUJG010000069 DASUJG010000000
VERSION     DASUJG010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609690"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1470)
                     /locus_tag="VFX38_01345"
     CDS             complement(<1..1470)
                     /locus_tag="VFX38_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017329837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX5313541.1"
                     /translation="MRGAAIARRKLTAAWGWPSANSDVCSIRVTRQSLSAAKRAVRAW
                     QTPKNRAQQRHASYTRKPGVIKSRSCAAENKLKGIAMHKLRSTLLAPLALVLMSFASL
                     AAFSQTPARVTTAATNIKHVFLILLENKNFSDTFETSTQDPYLRKTLVPMGALLTEYY
                     GTGHVSLDNYIALISGQAPTPDTDNDCLVRPRGALYIDVQQTGTTPDGQVIAAHGCIY
                     PAQVKTLADQLDAAGYDWKGYMEDMGNDPARESATCGHPRVGGPDDTSSAEAPSAAVP
                     LGDAYATRHNPFMYFHSIIDSPRCKTHVVNLNKLPADLKEVRTTPNFSLITPNLCNDA
                     HDGGGTGARGRGCANGDPGGLTSADAFLQRWVPKILASPAYRQDGLLIITFDESASAV
                     SQHVDEKTRKRIVDVTFSGKTCCRQQRGPNLENARPGSVVLSESEKALVRWVFDGYGG
                     DRIGALLLSPFAKPGSKSNTPYNHYSMLRSLEDIFALDGY"
     gene            1587..2183
                     /locus_tag="VFX38_01350"
     CDS             1587..2183
                     /locus_tag="VFX38_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313542.1"
                     /translation="MTEVRQRLMDIAARAVEATRAAAAGRASDRPAADAPDPRDATVA
                     RLEQEIEVERTKSAALLGTVKELEFKLGVLEKSYSKQLGDAKTRGEIAMQELSKLKAQ
                     LAETTDELRRTGEARDRLREMLAFDGRRIPPELRERQPGVEDTIARLLSLDSAAANAP
                     ANRKELPAEPIRAEPEPQPGDLLAPDLVFNPDDDEKEG"
     gene            complement(2251..>4274)
                     /locus_tag="VFX38_01355"
     CDS             complement(2251..>4274)
                     /locus_tag="VFX38_01355"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5313543.1"
                     /translation="SHDFWQSRFGSDPGVVGRVLTINSQPLQVIGIAPAGFSGTQIGA
                     RIRIFVPMTMHWQLDAFGPSDTPENRRFRFARLFARLRDGITATQAAALLNTVYARIV
                     KAEGPQSARMQQYLSRPLDLAPGARGQGTMQGASPPLTLLLAVTLLVLLIVCVNVANL
                     LLMRGASRAGEIAIRQSMGARRGRLIAELLSETAFPAAIGGILSLPVAAATLAAVAPI
                     LPASLAEGLGMHVGGAAVLFAALATVASVLVFGLFPALRASHTDPAIVMKSHAAQALR
                     GQGGARLRAALVTAQIAFSMVLLVLAGLFAESLGNIARIDLGMDVDSLVSFSVSPGSN
                     GYDAPRSRALYERITEALAAQPGVTAVGSAVLPVVTGANNSAPIQVEGFDNGGKRLFA
                     NFNFVGPSFFQTLSIPLLAGRTFAAADFATAPRAAIVNERFVRTYGLGRNAIGKLITF
                     PGNLRFEIVGVVANAAYSQVKGEGPPQFFLPRGRLDGSPFAGQEASSSFYVRAAIDPD
                     ALLKVIPRVVAGIDPTLPVTHLITMRQQVKQDIFVDRLVSILSASFAGLATLLAAIGL
                     YGVMAYNVTARTREMGLRLALGAEPADLRVMVLKRVGGMAVIGLSVGLVAAIGAGRAA
                     EALLYGLTGHDPVVLVGAALVLAAVVLAASYWPARRASSIAPMEALRHE"
BASE COUNT          783 a         1356 c         1419 g          716 t
ORIGIN      
        1 ataaccgtcg agcgcgaaga tgtcctcgag gctgcgcagc atcgaatagt gattgtaggg
       61 agtattcgac ttgctccccg gcttcgcgaa cggcgacagc agcagcgcgc cgatgcgatc
      121 gccgccgtag ccgtcgaaca cccagcgcac gagcgccttc tcgctttcgg ataaaacaac
      181 gctgccgggc cgggcattct cgagattggg cccgcgttgc tgacggcagc aggtcttgcc
      241 ggaaaacgtg acatccacga tccgcttgcg ggttttttca tcgacgtgtt ggctcacggc
      301 cgaggcgctt tcatcgaacg tgatgatgag cagaccgtcc tgccgataag cgggcgacgc
      361 gagaatcttc ggcacccagc gctgaagaaa agcatccgcc gacgtcagcc cgcccgggtc
      421 gccgttcgca cagcctctgc cccgggcgcc cgttccgccg ccgtcgtggg cgtcgttgca
      481 gaggttcggc gtgatcagtg agaagttggg cgtcgtccgc acttccttga ggtccgccgg
      541 caacttattg agattgacga cgtgagtctt gcaacgcggc gaatcgatga tggagtggaa
      601 gtacatgaac gggttgtggc gcgtggcata ggcgtcgccg agcggcaccg cggcgctcgg
      661 tgcctccgcg gagctcgtgt cgtcagggcc gccgacgcgc gggtggccgc aggtcgcgct
      721 ctcgcgcgcc gggtcgttcc ccatatcctc catgtagccc ttccagtcgt acccggccgc
      781 atcgagctgg tcggcgaggg ttttcacctg cgcggggtag atgcagccgt gcgctgcgat
      841 cacctgcccg tcgggcgtcg tgcctgtttg ttgcacatct atgtatagcg ctccgcgagg
      901 acgcacgagg cagtcgttgt cggtatccgg cgtgggcgcc tgcccgctga tgagcgcgat
      961 gtaattgtcg agactcacat ggcccgttcc gtagtactcg gtcagcagcg cgcccatggg
     1021 cacgagcgtc ttccgcaggt acgggtcctg tgtgctggtc tcgaaagtgt cgctgaagtt
     1081 cttgttctcg agcaggatga gaaagacgtg cttgatgttc gtcgcagccg tcgtgacgcg
     1141 ggcgggcgtt tgagagaaag cagcgaggct cgcaaacgac attaggacga gagcgagcgg
     1201 agcgaggaga gtgctgcgaa gcttgtgcat tgctattccc ttgagtttgt tctctgccgc
     1261 gcacgagcgc gatttgatca cccccggctt gcgggtatac gacgcgtggc gctgttgagc
     1321 ccggttcttc ggagtttgcc aagcccgaac ggcccgtttc gcagccgata ggctttgccg
     1381 agtgacccga atggagcata cgtcggagtt tgcgctcggc caaccccacg cggcggtcag
     1441 ctttcgccgc gcgattgccg cccctcgcaa tccagaccgg gacctttcgc tgtacgtcgg
     1501 tctgtgatgg acctcaatgt accctggaag cgttccaact aggcttaacc gcggaagcga
     1561 gaacgtttcg tataaaggaa tgacacgtga ccgaagtacg ccagcgcttg atggatatcg
     1621 ctgcccgggc agtcgaagcc actagggcgg cagcggccgg ccgggcatct gatcgtcccg
     1681 ccgcggatgc gccggacccg cgggatgcga cggtcgcgcg tctcgagcag gagatcgagg
     1741 tcgaacgcac caagtccgcc gcgctactcg gcaccgtcaa ggagctggag ttcaagctcg
     1801 gggtgctgga aaagagctac tcgaagcagc tcggcgacgc gaagacccgc ggcgaaatcg
     1861 ccatgcagga gctgtccaag ctcaaagctc aactcgccga gacgaccgat gagctgaggc
     1921 gcacgggaga ggcgcgcgat cggctgcgcg aaatgctcgc gttcgacggc cggcggattc
     1981 cgcccgagct gcgcgagcgg caacctggag ttgaggacac gatcgctcgg ctgctctcgc
     2041 tggacagcgc ggcggcgaat gcgccggcga atcgcaagga gctgcccgcg gagcccatcc
     2101 gcgccgaacc cgagccgcag cccggggact tactcgcgcc agacctcgtg tttaacccgg
     2161 acgatgacga gaaggagggt tagcgagcag cttctccgac tcgcgtctct ccggtttcat
     2221 cctgagttgc ggcggcaagc cgactacgcg ctactcgtgc cgcaacgcct ccatcggcgc
     2281 gatgctcgaa gcgcgtcgcg ccggccagta gctcgcagcg agcacgaccg cggccagcac
     2341 gagcgcggcg cccacgagca cgaccggatc atggcccgtg aggccgtaca acaatgcttc
     2401 ggcagctcgc ccggcgccga tcgccgcgac gagccctaca ctcaagccga tcaccgccat
     2461 gccgccgact cgcttcaata ccataactct caggtccgcg ggctccgccc cgagcgcaag
     2521 cctcaagccc atctcgcgcg tgcgcgccgt cacgttataa gccatgacac cgtagagtcc
     2581 gatcgccgcg agcagcgtcg cgagcccggc gaagctcgcg gagaggatcg agacgagcct
     2641 gtcgacgaaa atgtcttgct tcacttgttg acgcatcgtg atcaagtgag tgaccggcaa
     2701 ggtggggtcg atgccggcaa cgactcgcgg aatcactttg aggagcgcat cgggatcgat
     2761 agccgcgcgc acgtagaacg aagacgatgc ctcctgccct gcgaacgggc tgccgtcgag
     2821 ccggccgcgc ggcaagaaga actgcggcgg cccttcgccc ttgacctggc tgtacgcggc
     2881 gttcgcgacg acgccgacga tctcgaagcg gagattgccc ggaaacgtga tgagcttacc
     2941 gattgcattc ctgccgagac catatgtacg cacgaagcgc tcgttcacga tcgcagcacg
     3001 aggcgccgtt gcaaaatccg ccgcggcgaa ggtgcggccc gcgagcagcg gaatggaaag
     3061 cgtttggaag aacgaagggc ctacgaagtt gaagttggcg aagagccgct ttccgccgtt
     3121 atcgaagcct tctacctgta taggcgcgct attatttgcg cccgtgacga ccgggagcac
     3181 tgctgacccc accgccgtga caccgggctg tgcggcgagc gcctccgtga tgcgctcgta
     3241 aagcgccctg cttcgcgggg catcgtagcc gttcgagccg ggcgacacac taaagctcac
     3301 gagcgaatcg acgtccatcc cgagatcgat gcgcgcgatg tttcccaggc tctccgcgaa
     3361 gagcccggcc agaacgagca gcaccatgga gaacgcgatc tgcgccgtca cgagcgcagc
     3421 gcgtaggcgc gcgccgcctt gtccgcggag cgcctgcgcg gcatggcttt tcatgacgat
     3481 cgccgggtcg gtgtgcgagg cgcgcaacgc cgggaaaaga ccgaacacga gcacgctcgc
     3541 aacggtggcg agcgccgcga acagcacggc cgctccgcct acgtgcatgc cgagcccttc
     3601 ggcgaggctc gccggcagga tgggcgcgac ggcggccagc gtcgccgccg cgaccggcag
     3661 ggacaggata ccgccgatcg cggcggggaa ggcggtttcg gacagaagct cggcgatgag
     3721 acggccgcgg cgcgcgccca tggactggcg gatcgcgatc tcgcctgcgc gcgatgcgcc
     3781 gcgcatgagc agcaggttcg cgacgttcac gcagacgatc agcagcacga gtagcgtgac
     3841 tgcgagcagc aaggttaacg gcggtgaggc gccctgcatg gttccctggc cacgggcgcc
     3901 cggcgcgaga tcgagcggcc gcgacaagta ttgctgcatc ctcgcggatt gcgggccttc
     3961 ggctttgaca atccgagcgt aaaccgtgtt gagcagtgcg gcggcctgcg tggcggtgat
     4021 accgtcgcgc aagcgcgcga acaggcgcgc aaatctgaat cggcgattct ccggtgtgtc
     4081 ggatggcccg aaggcatcca gctgccagtg catcgtcatc ggcacgaaaa tccgaatccg
     4141 agctccgatc tgcgttccgg agaaccccgc cggtgcgatg ccgatgacct ggagcggctg
     4201 actgttgatt gtcaacacac gtccaacgac gcccggatcg ctgccgaatc ggctctgcca
     4261 aaagtcgtgg ctca
//
LOCUS       DASUJG010000070         2170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1120956, whole genome shotgun sequence.
ACCESSION   DASUJG010000070 DASUJG010000000
VERSION     DASUJG010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120956"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /gene="rfaE1"
                     /locus_tag="VFX38_01360"
     CDS             complement(<1..890)
                     /gene="rfaE1"
                     /locus_tag="VFX38_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HEX5313544.1"
                     /translation="MQPQIPEFAGLRILVAGDVMLDRYWSGATERISPEGPVPVVHVG
                     EMEERPGGAANVAANLASLGVATTLLGLVGVDPEAGRLAKLTEALGIATRFIARAEAP
                     TVAKLRVLSRHQQLLRLDFERGFDGAAADELADRFEAALAGCSIAVASDYAKGALGAI
                     TRMIAAARSAGLRLLIDPKGRDFDRYRGAWALTPNRAEFEAVAGACADDAELARRGEK
                     LRASLDLAALLITRGEEGLTLVGAGEPVLHLPTAARDVADVTGAGDTVIAVFAAGLAE
                     GLAAADAAKIANVAAGLVVAR"
     gene            951..1847
                     /gene="lpxL"
                     /locus_tag="VFX38_01365"
     CDS             951..1847
                     /gene="lpxL"
                     /locus_tag="VFX38_01365"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferase"
                     /protein_id="HEX5313545.1"
                     /translation="MRGMLPKNLYAPRYWPEWLMVGFFAILVRVPWRLRLAVGRTIGR
                     LARRFAGRRRHITAANLALCLPELDARERDALLEAHFESLGVGLIEMATAWWATDRNL
                     PPGLVLNGLEHLERALTGGRGALLLTAHFTTLEMGARVLNLHRSVTAVYRPHENPVLD
                     RVIRGGRLGRAGAMIERGDLRGMLRALRSGAVVWYAPDQAYLGPHGIEAPFFGIAAPT
                     NTATARIAAASRAPVLPFFVRGLPHRDGYQVDIEAPLADFPSGDALADATRVNTVLEA
                     GIRRAPSQYLWSHDRFKRFRRP"
BASE COUNT          327 a          760 c          721 g          362 t
ORIGIN      
        1 cgcgcgacga cgagaccggc cgccacgttg gcgatcttcg cggcatcggc ggcggcaaga
       61 ccctccgcga gccccgcggc gaagacggcg atgacggtat cgcccgcgcc ggtgacatcg
      121 gcgacgtcgc gcgccgccgt cggcagatgc aggacgggtt cgccggcgcc gacgagcgtc
      181 aggccttcct cgccgcgggt aatgagaaga gccgcaagat cgagcgacgc acgcagcttt
      241 tctcctcgcc gcgcgagttc tgcgtcgtcg gcgcaggctc ccgccaccgc ctcgaactcg
      301 gcgcgattgg gcgtgagcgc ccaagccccg cggtagcgat cgaagtcccg gcccttcggg
      361 tcgatcagca gccgcaggcc ggcgctgcgc gccgcggcga tcatgcgcgt gatcgctccg
      421 agcgctccct tggcgtagtc ggacgccacc gcgatgctgc agccggcaag cgcggcctcg
      481 aaacgatcgg cgagttcgtc ggcggcagct ccgtcgaaac cgcgctcgaa gtcgaggcgc
      541 aggagttgct ggtgccgcga gagcacccgc agcttcgcca ccgtcggcgc ctccgcccgg
      601 gcgatgaatc tcgtggcaat gccgagggcc tcggtgagct tcgcgagccg gcccgcttcc
      661 ggatcgacgc cgacgagtcc gagcagcgtc gtggcgactc cgaggctcgc gagattggcc
      721 gccacgttcg cggcgccgcc ggggcgctcc tccatctcgc ccacgtgcac gaccggcacc
      781 ggtccctcgg gagaaatgcg ttcggtagct ccggaccaat aacgatcgag catgacgtcg
      841 cccgcgacga gaatccgcag ccctgcaaac tcgggtatct ggggttgcat gaggatcagc
      901 gcgggagtcg gcgcgatcgt agcacagcgc cgggagcctc catcgctaca atgcgcggca
      961 tgctcccgaa aaatctgtac gcgccgcgct actggcccga gtggctgatg gtcggctttt
     1021 tcgcaatcct ggtgcgcgtt ccctggcgcc tgcgcctcgc cgtcgggcga acgatcgggc
     1081 gactcgcgcg gcgcttcgcc gggcggcgcc gccacatcac ggcggccaat ctcgctctgt
     1141 gcctgccgga actcgacgcc cgcgaacgcg acgcactgct cgaggcacac ttcgagtcgc
     1201 tcggcgtcgg gctcatcgaa atggcaaccg cttggtgggc caccgaccgg aatctgccgc
     1261 cgggtctcgt cctcaacggt ctcgagcatc tcgagcgagc cctcacgggc gggcgcggcg
     1321 cgctgctcct cacggcccac ttcacgaccc tcgagatggg cgcccgcgtt ctcaatctgc
     1381 atcgcagcgt tacggcggtg taccgcccgc acgagaatcc ggtactcgat cgggtcattc
     1441 gcggcgggcg cctggggcgc gccggcgcga tgatcgaacg cggcgatctg cggggcatgc
     1501 tccgggcgct gcgctccgga gccgtggtct ggtatgcgcc ggatcaggcc tatctcgggc
     1561 cgcacggcat cgaagctccg tttttcggga tcgcggcacc gacaaacacc gccaccgcgc
     1621 gcatcgcggc ggcgtcacgg gcgccggtgc tgccgttttt cgtgcgcggc cttccgcatc
     1681 gcgacggcta tcaggtggac atcgaggcgc cgctggcgga tttcccgagc ggcgacgcgc
     1741 tcgccgatgc aacccgggtg aacaccgttc tcgaagccgg aatccggcgt gcaccgtccc
     1801 agtacctctg gagccatgat cggttcaaac gcttccgtcg cccctgagcg gtgctgctga
     1861 aacttttccg ttatagaaca atagattggt gttccatagc cggcaacgac ggccggccgg
     1921 cagagatcgg gttcccatcg ttttctaatc caaatccgaa ttgattttcg tagccccata
     1981 aaatcaaacg gttttccact tgcatttctc gccgacttga tccatatttc ttgtgttgat
     2041 accgggagac ggtgatgaat ctgtccgcgc ccgcccgctt gcagcgtcga cgcatgaccg
     2101 cggcggcgag tgtgctggcc gcgctgttgc tgctcgcggc ccgtccggcc gcgccggcgg
     2161 cggccacggg
//
LOCUS       DASUJG010000071         3265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_630766, whole genome shotgun sequence.
ACCESSION   DASUJG010000071 DASUJG010000000
VERSION     DASUJG010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630766"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /locus_tag="VFX38_01370"
     CDS             complement(<1..1152)
                     /locus_tag="VFX38_01370"
                     /inference="COORDINATES: protein motif:HMM:NF004834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5313546.1"
                     /translation="MNTSETQCCIVGGGPAGMMLALILARAGIDVTVLEKHGDFLRDF
                     RGDTAQPSTLQILQDLGLMERFRALPVEWVDTLSVGAANGVFPLGDYRRLKPFSELAL
                     VPQWDLLNLLAEEGGRCPNYRLLMRTEGRETIEENGRIVGVRASGPEGEMEIRADLVV
                     GTDGRRSTMRRAAGFEPRELGAPMDVLWFRLSREASDPQTSGGFVGKGQLLVMLNRGS
                     YWQAGYVIAKGGIDALRARPLEEFQQRLGVLAPFLGERAREIRSWDDVSLLVVQVDRI
                     PRWHRPGLLLIGDAAHAMSPVGGVGVNVAIQDAVAAANILGPVLLAGRRPEETDLARV
                     QKRREWPVRALQRVQIMIQNRLISRALAADSGALTIGRLPRLLLRSGFVR"
     gene            complement(1296..1640)
                     /gene="rplS"
                     /locus_tag="VFX38_01375"
     CDS             complement(1296..1640)
                     /gene="rplS"
                     /locus_tag="VFX38_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX5313547.1"
                     /translation="MKQVIQELEREQLRSDIPEFAPGDTVVVQVKVSEGGRERLQAFE
                     GVVIARRRRGVGSSFTVRKVSHGEGVERVFMMHSPSIASVEVKRRGNVSRAKLYYLRE
                     LRGKSARIKEKV"
     gene            complement(1676..2383)
                     /gene="trmD"
                     /locus_tag="VFX38_01380"
     CDS             complement(1676..2383)
                     /gene="trmD"
                     /locus_tag="VFX38_01380"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX5313548.1"
                     /translation="MGPRFDVVTIFPEMIAGALAYGVVARALASGRARVECWNPRDWS
                     EDPHRSVDDRPYGGGPGMVMKFAPLAAAVRAAQAARGDAAPVVYLSPQGERFDDARAR
                     SLAAGAGAVLVCGRYEGVDERFVEACVDAELSIGDYVLSGGEAAALAVMDAVIRLVPG
                     VLGDEDSASEDSFVTGLLDCPHYTRPEEIENRRVPEVLLSGDHAAIRRWRREESQRRT
                     RERRADLVQSAGEPDFS"
     gene            complement(2403..2915)
                     /gene="rimM"
                     /locus_tag="VFX38_01385"
     CDS             complement(2403..2915)
                     /gene="rimM"
                     /locus_tag="VFX38_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018137643.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX5313549.1"
                     /translation="MSAGGSEEWIELGRISGFYGVRGWVRVFSHTDPRAGILSYSRWF
                     LGAGEERREYRVDDGRMQGGGVVAALIGVENREAARGLLGLAISVPRRELPPIEGFYW
                     ADLIGLAVENRTGIALGHIVAMLETGGHDVMVIADRGRERLIPWAPGRYVDGVSLAAG
                     RVTVDWDPDD"
     gene            complement(3017..>3265)
                     /gene="rpsP"
                     /locus_tag="VFX38_01390"
     CDS             complement(3017..>3265)
                     /gene="rpsP"
                     /locus_tag="VFX38_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003092643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX5313550.1"
                     /translation="IVVTDSRRARDGRFIERIGYFNPIATGGERRLVLDSDRAAFWQA
                     RGAQLSQRVAALVKESARPAGAPAVEPPATPAEAASEA"
BASE COUNT          584 a         1195 c          992 g          494 t
ORIGIN      
        1 gcgaacgaag ccggatcgca acagcaggcg cggcagcctg ccgattgtca gcgcaccgga
       61 atcggcggcg agcgcgcggg agatcaggcg attctgaatc atgatctgga cgcgttgcag
      121 cgctcgcacc ggccactccc gccgcttctg gacccgggcg agatcggttt cctcggggcg
      181 gcgcccggcg agcagaacgg gcccgagaat attggcggcg gcgacggcat cctgaatcgc
      241 gacgttgacg ccaacgcccc cgaccggcga catcgcgtgg gccgcatcgc cgatcagcag
      301 gagcccggga cgatgccagc gcgggatgcg gtccacctgc acgacaagga gactcacgtc
      361 gtcccaggaa cggatctcgc gggcacgctc gccgagaaag ggcgcgagca cgccgaggcg
      421 ctgttggaat tcttccagcg gccgcgcgcg cagcgcatcg atcccgccct tggcgatgac
      481 gtagcccgcc tgccagtagc ttccgcgatt gagcatcacc agcagttgtc ccttgccgac
      541 aaagcctccg ctcgtctgcg gatcgcttgc ctcgcgcgag agacgaaacc agagcacgtc
      601 catcggcgcc ccgagttcgc gcggctcgaa gcccgcggcc cggcgcatgg tcgagcgccg
      661 gccgtcggtg ccgacgacga gatcggcgcg aatttccatc tccccttccg gtcccgaagc
      721 gcgcaccccg acgatgcgcc cgttttcctc gatcgtctcg cgtccctcgg tgcgcatgag
      781 cagtcgataa ttcggacagc gcccgccctc ttcggcgagc aggttgagaa gatcccattg
      841 cggcacgagg gcaagttcgg agaacggctt cagccggcgg taatcgccca agggaaacac
      901 gccgtttgcg gctcccaccg aaagcgtgtc gacccattcg acggggagcg cgcggaaacg
      961 ctccatgagt cccaggtcct gcaatatctg gagcgtcgaa ggctgggccg tatcgccgcg
     1021 aaaatcgcgc agaaagtcgc cgtgcttctc gagcaccgtg acgtcaatgc cggctcgggc
     1081 gagaatcagc gcaagcatca tgccggcggg gcctccgccg acgatgcagc attgcgtctc
     1141 gcttgtgttc aggcgcggaa agccgacggc gggcgcagca cggaccccgg cagcggaacg
     1201 ggacggtacg gagtcaagtc ggccaactca cgacgtcgga ggaggcgagc cgcttgcacg
     1261 gaacgtggcg aagccacacg ccaactctcc gaaccctaga ccttttcctt gattcgtgcc
     1321 gacttgccgc gaagctctcg gagataatag agtttggcac ggcttacgtt gccgcggcgt
     1381 ttgacttcca cgctcgcgat cgacggactg tgcatcatga atacgcgctc gacgccctcg
     1441 ccgtgcgaaa ccttgcgtac ggtgaaggag ctgccgacgc cgcgccggcg ccgggcgatg
     1501 acgacgcctt cgaatgcctg caagcgttcc cgtccgccct cgctcacctt cacctgcacc
     1561 accaccgtgt cgccgggggc gaattcggga atgtcgcttc tgagctgctc gcgttccagc
     1621 tcctgaatca cctgtttcat cgtcctgctc cttcgactga ttgacaactc taccgtcaag
     1681 aaaaatccgg ctcgcccgca gactgcacca gatccgctcg ccgctcgcgc gtgcgccgct
     1741 gcgactcctc ccgccgccag cggcgaatgg cggcgtgatc gcccgaaagc agcacctcgg
     1801 gcacccggcg gttttcgatc tcctccggac gcgtgtagtg cgggcagtcg agcagtcccg
     1861 tcacaaaaga atcctcgctg gccgaatcct cgtcgccgag cacgcccgga accagacgaa
     1921 tcaccgcatc catcaccgcc agggccgcag cctcgccgcc cgagagcaca tagtcgccga
     1981 tcgaaagttc cgcgtccacg caagcctcga cgaaacgttc gtccacgccc tcgtagcgac
     2041 cgcaaaccag aaccgcacct gctccggccg cgagcgaacg ggctcgcgca tcatcgaagc
     2101 gttcgccctg cgggctgaga tagacgaccg gcgcagcatc gccgcgcgcc gcctgcgccg
     2161 cccgcaccgc ggccgccagc ggcgcgaatt tcatcaccat tcccggtccc cctccgtagg
     2221 gccggtcgtc cacgctgcga tgcggatcct cgctccagtc acgcggattc cagcactcga
     2281 cccgcgcgcg gccgctggca agcgcccgcg ccaccacccc gtaggcgagc gctccggcga
     2341 tcatctccgg aaagatcgtc accacatcaa atcgcggtcc catcgcctct gcaaccccga
     2401 cctcagtcgt ccggatccca atccaccgtg actcgcccag cggcgagaga cacgccgtca
     2461 acataccggc cgggggccca ggggatgagc cgctcgcgcc cgcgatccgc gatcaccatc
     2521 acgtcgtgtc cgcccgtctc cagcattgcg acgatatgtc ccaatgcgat ccccgtccga
     2581 ttctcaaccg caagtccgat gagatccgcc cagtaaaaac cttcgatcgg cggcaattcg
     2641 cgccgcggaa ccgagatcgc gagccccagc aggcctcgcg ccgcttcgcg attctcgacg
     2701 ccgatcaagg ccgctaccac accgccaccc tgcattcgcc cgtcatcgac acggtattcg
     2761 cgccgctctt cgcctgcgcc caggaaccaa cgggagtagg aaagaatgcc cgcccgtgga
     2821 tcggtgtgcg aaaaaacgcg cacccaaccg cgcacgccgt agaatccgct gatgcgcccg
     2881 agttcgatcc attcctcgga gccgcctgca ctcatcccgg aacccgccgc aggccgggcg
     2941 ccgccgcagg gcccgaaacc ttcatcaatt tctcccggcg atcggcggcg ccacggcgaa
     3001 cctgccggat gctctcctag gcttcgctcg cggcttccgc gggcgtggct ggcggctcta
     3061 ccgccggagc tcccgccggc cgggccgatt ccttgacgag agccgcgacc cgctgggaga
     3121 gctgcgcacc ccgcgcctgc cagaaggcgg cgcgatcgga atcgagaacg aggcgacgtt
     3181 cgcctccggt cgcgatggga ttgaaatagc cgatgcgctc gatgaagcgg ccgtcgcggg
     3241 cgcgccggct gtccgtcacc acgat
//
LOCUS       DASUJG010000072         3720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_264711, whole genome shotgun sequence.
ACCESSION   DASUJG010000072 DASUJG010000000
VERSION     DASUJG010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264711"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VFX38_01395"
     CDS             complement(<1..631)
                     /locus_tag="VFX38_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313551.1"
                     /translation="MAPPSTRRSTRAEWLQAFTDEPDVFFFRLDATARNALLVRLDAE
                     RIREAPFLDERALRGDEEGVWLPLDALFETSVCNRAPQGIILHCGHCGSTLISRLLGE
                     LPDARVLREPLVLHALAAEARLAGSAQAGLRADEFRRTLWLAESSFAKAPTPATRVIV
                     KHTSFTANLGVALLESGFAPTVLLLWIPLDEYLATMLRDPGLRQGLRQAA"
     gene            complement(634..2184)
                     /locus_tag="VFX38_01400"
     CDS             complement(634..2184)
                     /locus_tag="VFX38_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313552.1"
                     /translation="MRVDAPRHPEWLPFAGATALVTLAFTLGFANHGPIPSMETRFAV
                     AVQQMLAHHQWLVPEKNGLPYIEYPPFYYWLSMLGAKAGLPLLPAIRLPNLVALWVWL
                     AAVYGLGRRLLPTLPDWLLAVATVATPAVLYNFFIAQADGWLAAGVALALLGYVRKSQ
                     ERRFPWMLWSGAALAVFAKGPVGLIVPVLAIGLDLLLALLGGARRLRDLPAAIFALAP
                     IRGVALALAPLAAWYLACGLVVGWDFVRAALVYDNLTRYVAGAGGHNNPWWLFAQTIW
                     GDYFPWIFALPIGLVLAARRLREAEPRLLFSWAVTTLLFFSASSSKQSKYILPAAPAF
                     VALAFLALAAAARRRPRWLRRGALAWSLFVLLVFTVVVGAWLPFAGPGIDDNAAYARL
                     RGTVSAQPGELVMYGWPRSLVLYQLGAPMPWLRDARDVYAAVHARRLASGDYLLVPHD
                     NLASYGARGAFALQPTPAPPYFERVMTLSSKGGIEVYRVLPSAAAAPIPPTPKPPPAP
                     WWARFDTD"
     gene            2352..3422
                     /locus_tag="VFX38_01405"
     CDS             2352..3422
                     /locus_tag="VFX38_01405"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5313553.1"
                     /translation="MKVTGDWRLHELLGSRNDALRRLESFDARPDDVWDFTGLDSLDS
                     AGALLFWRCWKGKEPDTIEGLAQHAHWIQRFADIHLQRPALPGRFAWVARILPGFGAA
                     LAGFGQQCYGAVLTGGQFVADIGHCIANPRMFPLREFSATVYRTGSQALFLNAFLGLI
                     IGFVLTWQIGGQIAQFGLNQAVIGAIGLAFTRELGPFIAALVLVGRTGSSMTAGIGAM
                     RITEEIDALRAFGVSPTLRVVMPNILGMTIVMPLLVVWTDFWGVIGGVYISSIKLGVT
                     WQMWLHNFPGSVPIEDYFIGLGKGALFGLIVGITSAYYGLTAPPDTQGLTRHITRSVV
                     VGLALVLVIESGFGILFSGVGL"
     gene            3419..>3720
                     /locus_tag="VFX38_01410"
     CDS             3419..>3720
                     /locus_tag="VFX38_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5313554.1"
                     /translation="MSEKEAVVHVQDLQTRIGDNLIHEDIDLDAYRGEVLAIIGTSGG
                     GKTTLLREITGLSEPTKGTVEIFGTDIHKLDWRERRKLRSRWGIMFQQGALFTALSV"
BASE COUNT          710 a         1167 c         1277 g          566 t
ORIGIN      
        1 atgccgcctg ccgcaacccc tgccgcaggc cgggatcgcg gagcatcgta gcgagatatt
       61 cgtcgagagg aatccacagc aaaagcaccg tgggcgcgaa acccgattcg agcagcgcaa
      121 ctccgagatt ggccgtgaaa ctcgtgtgct tgacgattac gcgcgttgcc ggcgtcggcg
      181 ccttcgcaaa cgaactctcg gcgagccaga gcgtgcggcg gaattcgtcg gcgcgcaggc
      241 ccgcttgcgc actgccggcg aggcgcgcct ccgccgccag tgcgtgcagc acgagcggtt
      301 cgcgcagcac gcgggcgtcg ggcagttcgc cgagcaggcg cgagatcagg gtggagccgc
      361 aatggccgca gtggaggatg atcccctgcg gagcgcggtt gcacacggac gtctcgaaca
      421 gggcgtcgag gggcagccag acgccttcct cgtcaccccg gagggcgcgc tcgtcgagaa
      481 acggggcttc gcggatgcgc tcggcatcga gccggaccag gagcgcgttg cgggcggtgg
      541 catccagacg aaagaaaaaa acatccggct cgtccgtgaa ggcctgcaac cactcggcac
      601 gggtagagcg acgcgtggaa gggggtgcca tcctcaatcc gtatcgaagc gcgcccacca
      661 cggtgcgggc ggcggcttgg gcgtcggcgg aatcggcgcg gccgcggcgc tcggcaggac
      721 ccggtagacc tcgatcccgc ctttgctgct gagagtcatg acgcgttcga aatagggggg
      781 agcaggcgtc ggctgcagcg caaaagcgcc gcgcgccccg tatgaagcga ggttatcgtg
      841 agggacgagc aggtaatcac cgctcgccag ccgtcgcgca tgcaccgcag cgtacacatc
      901 gcgcgcgtcg cgcagccacg gcatcggcgc tccgagttga tagagcacca gcgaacgcgg
      961 ccaaccgtac atcacgagtt ctccgggctg cgcggaaacg gtacctctga ggcgcgcata
     1021 ggcggcattg tcgtcgattc ccggaccggc gaagggcagc caggctccga ccaccaccgt
     1081 aaagaccagc agaacgaaga gggaccaggc gagcgcgccg cgtcgcaacc agcgaggacg
     1141 acgacgcgcc gcggctgcga gcgcgaggaa tgcgagtgcg acgaaggccg gcgcagccgg
     1201 cagtatgtat ttgctctgtt tggaagagga agccgagaaa aacagcaggg tggtcaccgc
     1261 ccaggagaaa agcagtcgcg gctcggcctc gcgcaggcgc cttgccgcga gaaccaatcc
     1321 gatcgggagc gcgaagatcc agggaaaata atctccccag atcgtctgcg cgaacagcca
     1381 ccagggattg ttgtgtccgc ccgcgcccgc aacgtagcgt gtgaggttgt cgtataccag
     1441 tgcggcgcga acgaaatccc agccgacgac cagaccacag gcgagatacc atgccgcgag
     1501 cggcgcgagc gcgagcgcga cgccgcgaat cggtgcgagc gcgaagattg ccgccggaag
     1561 atcccgcaat cgtcgcgctc cgccgagaag cgcaagcagg agatcgaggc cgatggcgag
     1621 cacgggaacg atcagcccga ccggaccctt ggcgaagacg gcgagagcgg cgccgctcca
     1681 gagcatccac gggaatcggc gctcttgcga tttgcgaacg tagccgagca gtgcgagagc
     1741 aaccccggcg gcgagccagc cgtcggcttg cgcgatgaag aagttgtaga gcacggcggg
     1801 cgtagcgacc gtcgctacgg cgagcagcca atcgggcagg gtcggcagca gccgccgccc
     1861 gaggccgtag accgcagcga gccacaccca gagcgcgacg agattcggaa ggcgaatcgc
     1921 cggaagcagc ggcagcccgg ccttggcgcc cagcatcgac agccagtagt aaaacggcgg
     1981 gtattcgatg tagggcagtc cgttcttttc gggcaccagc cattgatgat gcgcgagcat
     2041 ctgctgcact gcgacggcga agcgcgtctc catcgacgga atggggccgt gattggcgaa
     2101 gccgagcgtg aaggcaagcg tgacgagcgc ggtggctccg gcaaacggca gccactcggg
     2161 atggcgcggg gcgtcgactc gcatgcaaag gcaccgctgg aacggcgcga ttgtcgcaca
     2221 ggccgcaggg tcgagcgggc tgcctgagcc cgacgatatg gtggtttata ctgccgttgg
     2281 gcactccggc gaaccttgcg atgggtgcgg cgccgcggct tgaaattcaa gacgagccaa
     2341 gccaacgccg agtgaaggtc accggcgact ggcgcttgca tgaattgctc ggttcccgca
     2401 acgatgcatt gcggcgcctg gaatcgttcg atgcccgtcc ggacgacgtg tgggacttca
     2461 cggggctcga cagtctcgac agcgcgggtg cgctcctttt ctggcgctgc tggaagggca
     2521 aagaacccga taccatcgag ggactggccc agcacgcgca ctggatccag cgattcgccg
     2581 acattcatct ccagcgcccg gcgcttcccg gccgcttcgc ctgggtcgcg cgcatcctgc
     2641 ccggcttcgg cgcggccctg gcgggctttg gacagcagtg ttatggcgcc gtcctcaccg
     2701 gcggccagtt cgtcgcggat atcgggcatt gcatcgccaa cccccgcatg ttcccgttac
     2761 gggaattctc ggcaacggtg taccgcaccg gctcccaggc gctgtttctg aacgccttcc
     2821 tgggattgat catcgggttc gtgctgacct ggcaaatcgg cggacagatt gcccagttcg
     2881 gcctgaacca ggccgtcatc ggcgcgatcg gactcgcctt cacgcgcgag ctcgggccgt
     2941 tcattgcggc gctggtgctg gtaggacgca ccgggagctc catgaccgcg ggaatcggcg
     3001 cgatgcgaat aaccgaagag atcgacgcct tgcgcgcctt cggagtctcg ccgaccttgc
     3061 gcgtcgtcat gccgaacatt ctggggatga cgatcgtgat gccgctgctc gtggtgtgga
     3121 ccgacttttg gggggtgatc ggcggggtgt acatctccag catcaagctc ggggtgacct
     3181 ggcagatgtg gctgcacaac ttccccggtt cggtgccgat cgaagactat ttcatcgggc
     3241 tcggcaaggg cgcattgttc ggcctgatcg tcgggattac cagcgcctat tacgggctca
     3301 ccgcaccgcc cgacacgcag ggactcactc gccacatcac ccgttcggtg gtggtcggac
     3361 tcgccctggt gctggtgata gaaagcggct tcgggatcct cttcagcggg gtcgggctgt
     3421 gagcgaaaag gaagccgtgg tgcacgtgca ggatttgcag acccgcatcg gcgacaatct
     3481 catccacgaa gacatcgacc tcgatgccta ccgcggcgaa gttctcgcga tcatcggcac
     3541 cagcggcggg ggcaagacca cgctgctgcg cgagatcacc ggcctgagtg agcccacgaa
     3601 aggcaccgtc gagattttcg gaaccgacat ccacaagctc gattggcgcg agcgacgcaa
     3661 actgcgcagc cgctggggca tcatgtttca acagggcgcg ctgttcactg cgctcagcgt
//
LOCUS       DASUJG010000073         2430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_20368, whole genome shotgun sequence.
ACCESSION   DASUJG010000073 DASUJG010000000
VERSION     DASUJG010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20368"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VFX38_01415"
     CDS             <1..382
                     /locus_tag="VFX38_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008381760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX5313555.1"
                     /translation="KDPQLAIIRNARRLLGERLVRVAKPSPILDPASGPLIAVRLSIL
                     TRKSLGGLETDLDARVLDSRGEPITGLYAAGEVAGFGGGGMHGYRALEGTFLGGCLFS
                     GRIAGRAAARFAGGIAAEAAAAGR"
     gene            432..1073
                     /locus_tag="VFX38_01420"
     CDS             432..1073
                     /locus_tag="VFX38_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313556.1"
                     /translation="MLNVRIESVAPAFESAPVLTSLIRLVTRTVAMGLMRGSPIDRLD
                     AKAIGRVLDALQRGGLIGTQRTHLEGLLQPAKGKGGLPGDAGPALERLVDLLEESPVP
                     TSEWPAMREVLGDVALSALLGISASSLRRYANDERATPPTVADRLHWVAMVIADLAGS
                     YNDFGMRRWFERGRSQLGGRSPRGALGTRWSPGDPAALRIRTLAASLAGAGAT"
     gene            1070..1621
                     /locus_tag="VFX38_01425"
     CDS             1070..1621
                     /locus_tag="VFX38_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RES domain-containing protein"
                     /protein_id="HEX5313557.1"
                     /translation="MSRIGFRHVDSRYPFVRNDADQPAARWHAEGTGPANSFADTPAG
                     AWAEFLRHEEIRDAADLPGIARSLWAVELPETAYASPALPPSVLEGDRSSYPACRAEA
                     ASLRARGAERIEAPSAALLPGGARGWIANPVQHEAPGARNGLVWILFGDASHLVGWPA
                     VESGSPPARVLPLVRHFPSSRLR"
     gene            1651..>2430
                     /locus_tag="VFX38_01430"
     CDS             1651..>2430
                     /locus_tag="VFX38_01430"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX5313558.1"
                     /translation="MSTPMVFVVGTGRCGTHTLWKLFESVPNTLSTHEGTGIVKSGPP
                     AHAGKRVGLGCMPELNSFLYHYASEAEFERTFAPDAAMTALMDGCFAGRASAVAWCAA
                     HGIAYCDANPFAFNFINYLHARYPHAKFIHLVRDGYACVRSWFRRDASTYPDDVPSVS
                     SAIEWLLAKPVPFPSETAYAKWASFDRVQKIAWFWSTVNANIARRLERVPAQNWMVVR
                     VEDVDAANAGAILDFCGLPRQFAPEALAPEDSSQSEGRELEW"
BASE COUNT          373 a          810 c          832 g          415 t
ORIGIN      
        1 caaggatccg cagctcgcga tcatccgcaa cgcacggcgt ctcctcggcg agcggctcgt
       61 tcgcgttgca aaaccgtcgc cgatcctcga tccggccagc gggccgctga tcgccgtgcg
      121 cctcagcata ctgacgcgca aatcgctggg gggactcgaa accgatctcg acgctcgcgt
      181 gctcgattcg cgcggcgagc ccattacggg cctctacgcc gcgggcgagg tggcgggctt
      241 cgggggcggt ggaatgcatg gctaccgcgc gctcgaaggc acgtttctcg gcggctgcct
      301 cttcagtgga aggatcgccg gcagggcggc ggcgaggttc gccggaggaa tcgctgccga
      361 agctgctgcc gccggccgtt gatctgcaaa tgagcgttgt cgccgggcgc tgaccgggat
      421 atattcggaa tatgttgaat gtccgaatcg agtccgtcgc accggccttc gagtccgcac
      481 cggtgctcac ctcgctcatt cgcctggtga cgcgaaccgt cgcgatgggg ctcatgcgcg
      541 gaagtccgat cgaccggctc gacgccaaag cgatcgggcg cgtgctcgac gccctgcaac
      601 gcggcggatt gatcgggacc cagcgcacgc acctggaggg gctgttgcag cctgcgaagg
      661 ggaaaggcgg cttacctggc gatgccggcc cggcgctcga gcgtctcgtc gatcttctcg
      721 aggagtcgcc ggtgcccacc tccgagtggc cggcgatgcg cgaagtgctc ggtgacgtcg
      781 cgctgtccgc cttgctcggc atctcggcct ccagtctcag gcgctacgcg aacgacgagc
      841 gagcgacgcc gccgacggtc gccgatcgcc tgcactgggt cgcgatggtg attgccgacc
      901 tcgccggaag ctataacgat ttcggcatgc gccgctggtt cgagcggggc cgcagtcaac
      961 tcggcggccg cagtccccgc ggcgcgctcg ggacacggtg gagtcccggc gatcccgccg
     1021 cgctgcgcat tcgtacgctg gcagcaagcc tcgcgggcgc cggagcaacg tgagccggat
     1081 tggatttcgt cacgtcgatt cgcggtatcc gttcgttcgc aacgatgcgg atcagccggc
     1141 tgcacgctgg cacgcggaag gcacggggcc cgcgaactcc ttcgccgaca cgcccgccgg
     1201 ggcctgggcc gagttcctgc gccatgagga gattcgcgac gcagcggatc tccccggcat
     1261 cgcacgttcg ttgtgggcag tcgaacttcc cgagaccgcg tacgcgtcac cggcgctccc
     1321 accgagcgtg ctcgaaggcg accgatcgag ctatccggcg tgccgggctg aggcggcttc
     1381 attgagggca cgaggcgccg agcggatcga agccccatcg gccgcgctgc tgccgggagg
     1441 cgctcgcgga tggatcgcca atccggtcca gcacgaggct cccggcgcgc gcaacggact
     1501 tgtctggatc ctgttcggcg atgcgagcca cttggtcggc tggcccgccg tcgagtcggg
     1561 ttcgccgccg gcgcgggtac tgccgctcgt gcgccatttt ccgtcgtcga ggttgcgatg
     1621 aacacgagcc tcgccgcgga atcgcggccc atgtcgaccc cgatggtctt cgtcgtcggc
     1681 accggtcgct gcggcacgca cacgctctgg aagctgttcg aatcggtgcc caacacgctc
     1741 agcacacacg aaggcaccgg gatcgtgaag agcggtccgc cggcacatgc agggaagcgt
     1801 gtcggacttg gctgcatgcc ggagttgaac agctttctct accactacgc gagcgaggcc
     1861 gagttcgagc ggaccttcgc ccccgacgcg gcgatgaccg cgctaatgga cggatgcttc
     1921 gcgggacgcg cgagcgcagt cgcgtggtgc gcggcgcacg gcatcgccta ttgcgacgcc
     1981 aacccgttcg ccttcaactt catcaactac cttcacgcgc ggtatccgca cgcgaagttc
     2041 atccatctcg tgcgcgatgg ctatgcctgc gtgcgctcgt ggttccggcg cgacgcgtcg
     2101 acttatccgg acgacgtgcc aagcgtttcg tcggcgatcg agtggttgct cgcgaagccg
     2161 gtgccgttcc cgtccgagac cgcgtatgcg aagtgggcga gcttcgaccg cgtacagaag
     2221 atcgcctggt tctggagtac ggtcaacgcg aacatcgcac gccgcctcga gcgcgttccg
     2281 gcgcagaact ggatggtcgt tcgagtcgaa gacgtggacg cggccaatgc cggcgcaatc
     2341 ctcgatttct gcggtctgcc gcgacagttc gcgcccgaag cgctggcacc tgaggactcg
     2401 tctcagtcgg aaggtcgcga gctcgagtgg
//
LOCUS       DASUJG010000074         3024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_509782, whole genome shotgun sequence.
ACCESSION   DASUJG010000074 DASUJG010000000
VERSION     DASUJG010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509782"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VFX38_01435"
     CDS             <1..315
                     /locus_tag="VFX38_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX5313559.1"
                     /translation="AGYGERLLGAWPAMRDRYRDRSPLARVDEITRPVIFFQGLDDPV
                     VPPAQSERMAAALAARGIAVAYERFAGERHGFRRPASIARALAAELGFHARVLGLHAA
                     PV"
     gene            complement(346..1401)
                     /locus_tag="VFX38_01440"
     CDS             complement(346..1401)
                     /locus_tag="VFX38_01440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX5313560.1"
                     /translation="MNITPTLRLLRERSASDLYLSANAIPRMRVEGKIETIGETPLGE
                     EEIAGIAAEAMSGPQRERFRNELQVEFGVEDRDSGRFRFTVFRQRGTTAIAIRYIPTE
                     IPGLSTLKLPPILGELAMSQRGLILVVGVSGAGKSTTLAAMLDKRNRECADHIITIED
                     PIEYVHRHRLSVVNQRELFVDALSYPLAMRSALRSAPDIVMVGEIHDRATMSAVINLA
                     NTGHLALSTLHTTNAAQTLDRVLSMFPELMQQQVRLDLSASLRAVVSQRLLNGTDGRL
                     LPAVEVMLNTPYIAELIRDNRLHELREAMEESTEPGMQSFDQALSTLYQAGRITLDEA
                     LANADSRANLEARIHFA"
     gene            complement(1439..2602)
                     /locus_tag="VFX38_01445"
     CDS             complement(1439..2602)
                     /locus_tag="VFX38_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX5313561.1"
                     /translation="MADSELAARRMQELLRGMIERGASDLFVTAGAPPAIRVDGEVHS
                     LPGPALTSEESAALVRAIMNDRQNREFDATKECNFAISPQDIGRFRVSAFVQRGRQGA
                     VLRTISATIPTVEELNLPAMLKDIVMTPRGLVIVVGATGAGKTTTLAAMMDHRNHNSH
                     GHIVTVEDPIEYLHEHAGCVVTQREVGVDTTEWRTALKNVMRQAPDVVQIGEIRDRET
                     MEQAVIFSETGHLCLATLHATNANQALDRIINLYPQERRQQLLMDLSFNLKAVVSQRL
                     LRRAGGEGRIPAVEILINTPLVSDMIFKGDVTGLKEIMARSAGAGMQTFDQSLFGLYE
                     AAAVSYEEAIRNADSRNELRLRIKLESKRETRRFDDDLESVTLESDGEGRRIG"
     gene            complement(2626..>3024)
                     /locus_tag="VFX38_01450"
     CDS             complement(2626..>3024)
                     /locus_tag="VFX38_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX5313562.1"
                     /translation="ALTAAETGHLVFATLHTSSAAKTIDRIVDVFPAGEKEMVRSMLS
                     ESLRAVIAQTLLKKTGGGRIAAWEIMIGTPAVRNLIREGKVAQLYSTIQTSQGVGMQT
                     LDQSLKELLRHNLIGREDARLKAQIKENFL"
BASE COUNT          467 a          965 c         1006 g          586 t
ORIGIN      
        1 gccggatacg gcgaacggct gctcggagcc tggccggcga tgcgcgatcg ttaccgggat
       61 cgctccccgc ttgcgcgcgt cgacgagatc acgcggccgg tgattttttt ccagggtctc
      121 gacgatccgg tggtgccgcc cgcgcaaagc gagcgcatgg ccgcggcgct cgccgcgcgc
      181 ggaatcgcag tcgcttatga gcgatttgcc ggcgagcggc acggctttcg ccgccccgcg
      241 agcatcgccc gggcgctcgc ggccgaactc ggctttcatg cccgcgtcct cgggctgcac
      301 gcggcgccgg tttaatactt cgcgcctggt gaggacgtgg gagcgtcagg cgaagtggat
      361 gcgcgcctcg agattggccc gcgaatcggc gttcgcgagt gcctcgtcca gggtgatgcg
      421 ccccgcctga tagagcgtcg agagcgcctg atcgaaactc tgcatgccgg gttcggtcga
      481 ttcttccatc gcctcacgta gctcgtgcag gcgattgtcg cggatgagtt cggcgatgta
      541 gggcgtattg agcatgacct cgacggccgg gagcaggcga ccgtcggtgc cgttcagcag
      601 gcgttgcgag acgacggcgc gaaggctcgc gctcagatcg aggcgcactt gctgctgcat
      661 cagttccggg aacatgctga gcacccgatc cagcgtctgc gccgcgttcg tcgtgtgcag
      721 cgtggagagc gcgaggtgac cggtattggc gagattgatc acggcgctca tcgtcgcgcg
      781 gtcgtggatt tcccccacca tcacgatgtc gggcgcggag cgcagggcgc tgcgcatggc
      841 gagagggtag gacagcgcgt cgacgaagag ttcgcgctgg ttcacgaccg agaggcgatg
      901 ccgatggacg tactcgatgg gatcctcgat cgtgatgatg tgatcggcgc attcgcgatt
      961 gcgcttgtcg agcatcgcgg cgagcgtcgt ggactttccc gcaccgctca cgccgaccac
     1021 gagaatgagg ccccgttgcg acatcgcgag ttcgcccaga atcggcggga gcttgagggt
     1081 cgaaagcccc gggatttccg tcggaatgta gcggatcgcg atcgcggtcg tgccgcgctg
     1141 gcggaacacg gtgaagcgga aacggccgct gtcgcgatct tcgactccga actcgacctg
     1201 caattcgttg cggaagcgtt cgcgttgcgg cccgctcatc gcttcggcgg cgatgcctgc
     1261 gatttcctcc tcgccgagcg gcgtttcgcc gatcgtctcg atttttccct cgacccgcat
     1321 ccgcgggatc gcattggcgc tgagatagag atccgaagcc gagcgctccc gcagaagccg
     1381 cagagtcgga gtgatattca tgctgctttc ccctctgctt gcgcgggtcc tcgcggcctc
     1441 aaccgatgcg ccggccttcc ccgtcgctct cgagcgtcac cgactcgagg tcgtcgtcga
     1501 agcggcgggt ttcgcgtttg ctctcgagct tgatccgcag gcgcagctcg ttgcgcgaat
     1561 cggcgttgcg gatcgcctct tcgtaggaga ccgcggccgc ctcgtacaga ccgaagagcg
     1621 actgatcgaa ggtctgcatg ccggccccgg cggatcgcgc catgatctcc ttgagccccg
     1681 tcacgtcgcc cttgaagatc atgtcggaga cgagcggcgt gttgatcagg atctccacgg
     1741 cgggaatgcg gccttcgcca ccggcccggc gcagaaggcg ctgcgagacg accgccttca
     1801 gattgaacga aagatccatc agcaattgct ggcgccgctc ctgcggatag agattgatga
     1861 tgcgatcgag ggcctgattc gcgttggtcg catgcagcgt cgcgagacag agatgcccgg
     1921 tttccgagaa gatcaccgcc tgctccatcg tctcgcgatc gcggatctct ccgatctgga
     1981 ccacgtcggg ggcctggcgc atgacgtttt tgagcgccgt gcgccactcg gtcgtgtcca
     2041 ccccgacttc gcgctgcgtg acgacgcagc ccgcatgttc gtgcaggtac tcgatgggat
     2101 cttcgaccgt gacgatgtgc ccgtgagaat tgtggttgcg atgatccatc atcgccgcaa
     2161 gagtggtggt cttgccggcc ccggttgccc ccacgacgat caccagaccg cggggcgtca
     2221 tcacgatatc cttcagcatc gcgggcaggt tgagttcctc gacggtcgga atggtggcgc
     2281 tgatcgttcg cagcaccgcg ccctggcggc cgcgctgcac gaaggcgctc acgcggaagc
     2341 gcccgatgtc ctgcgggctg atggcgaaat tgcattcctt ggtcgcgtcg aactcgcggt
     2401 tctgccgatc gttcatgatc gcccgcacca gggcggcgct ctcctcgctc gtgagcgccg
     2461 gcccgggaag gctgtgcact tcgccgtcca cccggatcgc cggcggtgcc ccggcggtga
     2521 cgaagagatc cgaggcgccg cgctcgatca tcccgcgcag aagttcctgc atgcgtcggg
     2581 cggccagttc cgaatccgcc atcccgtttg tcgccggctc cgtattcaca ggaagttctc
     2641 cttgatctgg gccttgaggc gcgcatcctc gcgaccgatc agattgtgcc gcagcaattc
     2701 cttgagcgac tgatcgagcg tctgcatgcc gacgccctga ctggtctgga tcgtcgagta
     2761 gagctgggcg accttgccct cgcggatgag attgcgcacc gcgggggtgc cgatcatgat
     2821 ttcccaggcc gcgatccgtc cgcccccggt ctttttgaga agcgtctggg cgattaccgc
     2881 ccgcagcgat tcggaaagca tgctgcgtac catttccttc tcgccggccg gaaacacgtc
     2941 cacgatccga tcgatggtct tcgccgcgga gctggtatgc agggtggcga agacgagatg
     3001 accggtttcc gccgcggtca gcgc
//
LOCUS       DASUJG010000075         3168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_20645, whole genome shotgun sequence.
ACCESSION   DASUJG010000075 DASUJG010000000
VERSION     DASUJG010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20645"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..363)
                     /locus_tag="VFX38_01455"
     CDS             complement(142..363)
                     /locus_tag="VFX38_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313563.1"
                     /translation="MHFDTADLIASVIFGSLGSGYLLYGRRAERPAFLIAGAALVIFP
                     WFVTNLVLLIAIGAALLLAAPFAAWWFEF"
     gene            complement(404..1621)
                     /gene="gspF"
                     /locus_tag="VFX38_01460"
     CDS             complement(404..1621)
                     /gene="gspF"
                     /locus_tag="VFX38_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HEX5313564.1"
                     /translation="MGAFEYTVLDTRGREKKGIIEGDTARAVRQKLREQGLIPLAIET
                     AAARESRRARTGFSLRRRISSSDLALITRQLATLVASGLPVEEALRASAEQNDKPRLK
                     SMIMAVRGKVMEGHSLAHGLADFPHVFPEIFRATVTAGEQTGHLDAVLERLADYAESR
                     QDLRQKLALALIYPILLTVVATAIVIGLMTYVVPQVVQVFVTINAQLPLVTRILITLS
                     EGLRSWGWLILILLVLAAITIRQMLKRPGPRHRFHQWQLHAPLIGRLVRGINTARFTR
                     TLSILAGSGVPLLEALRISGDVVSNLPMRDAVMEAAERVREGAAIHKALAQRKLFPPM
                     TIHLIASGEQSGALDDMLERAAINQERELSGLIAGMLAILEPGMILLMGAAVLFIVLA
                     ILLPIFDLNQLVH"
     gene            complement(1753..3168)
                     /gene="gspE"
                     /locus_tag="VFX38_01465"
     CDS             complement(1753..3168)
                     /gene="gspE"
                     /locus_tag="VFX38_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HEX5313565.1"
                     /translation="MRELADDHALIAHRRDADPLALLELRRLFSRPLRLEAIDREEFD
                     RLLQQSYEEHQTIEGFETLEARTDLSSVADQLPEPEDLLEADDDAPIIRLINALLSEA
                     VRENASDIHIEPFEDYLVVRMRVDGVLREVLKSRRAVAPLVVSRVKVMARLDIAEKRL
                     PQDGRISLRLAGRPVDVRVSTIPCGHGERVVMRLLDKQAGTISLQHLGMPAPTREVLE
                     DLIRKPHGILLVTGPTGSGKSTTLYAALMRLNDHSRNIMTVEDPIEYYIDGIGQTQVN
                     TRVQMTFARGLRAILRQDPDVVMVGEIRDLETAEIAIQASLTGHLVLSTLHTNTAVGA
                     IPRLDDMGVESFLLSSSLIGLLAQRLVRLLCADCREPYRPEERERKLIGFADKGPLTF
                     YRAVGCEACNRTGYRGRTGIYELVPVDDKMRRLIHDRTGEQDLEAYARTRSAGIRAHG
                     RERVLAGETSVEEVLRVTRED"
BASE COUNT          577 a          977 c         1078 g          536 t
ORIGIN      
        1 gctcgcgctt cgtcgccttg cgatacttct cgaaaccggc ctcggcgaaa gtgggctggc
       61 gcattagatc atcctccaaa gtgcgggagc aaaaacaatg tctactatga tcgccaatcc
      121 ggaaaattgt tcagagattc cctagaattc aaaccaccac gcggcaaacg gcgcggcgag
      181 caggagcgcg gcgccgatcg cgatcagcag gacgaggttc gtgacgaacc aggggaaaat
      241 gacgagtgcg gcgccggcaa tcagaaaggc cggcctttcg gcgcgcctgc cgtacagcag
      301 gtaaccgctt cccagcgaac cgaagatcac gctcgcgatg agatcggccg tatcgaagtg
      361 catccgcgtc tcgggccggg caccgcggtg ggcgacggtc cgctcagtga acgagctggt
      421 tgagatcgaa gatcgggagc aggatcgcga gcacgatgaa gagcacggcg gctcccatga
      481 ggagaatcat ccccggctcc aggatcgcga gcatccccgc gatcagcccg gaaagttcgc
      541 gttcctgatt gatcgcggcg cgttcgagca tgtcgtcgag ggcgcccgac tgctcgccgc
      601 tcgcgatgag atggatcgtc atgggaggaa agagcttgcg ttgcgcgagc gccttgtgga
      661 tggcggcgcc ttcgcgcacc cgctcggcgg cttccatcac cgcgtcgcgc atcgggagat
      721 tcgagacgac gtcgccggag atgcgcagcg cctcgagcag cggcaccccg cttccggcga
      781 gaatcgagag cgtgcgcgtg aagcgcgcgg tgttgatccc gcgcacgagg cgcccgatga
      841 gcggagcgtg caactgccat tggtggaagc ggtggcgcgg accgggacgc ttgagcatct
      901 ggcggatcgt gattgccgca aggacgagca gaatcagaat gagccagccc caggaacgaa
      961 gcccttcgga aagcgtgatc aggattcgcg tgacgagcgg gagctgcgcg ttgatggtaa
     1021 cgaatacctg caccacctgc ggcacgacgt aggtcatgag cccgatcacg atggcggtgg
     1081 cgaccaccgt gaggagaatc ggatagatga gcgcaagcgc gagtttctgg cggagatcct
     1141 ggcggctttc cgcgtagtcg gcgagccgct cgagcaccgc gtcgagatgc ccggtctgct
     1201 cgcccgccgt caccgtcgcg cggaaaatct cgggaaagac atgcggaaag tcggcgaggc
     1261 cgtgcgcgag cgaatgtccc tccatgacct tgccgcgcac cgccatgatc atgctcttca
     1321 ggcgcggctt gtcgttctgt tcggcgctcg cgcgcagtgc ctcctcgacc ggcaggcccg
     1381 aggcgaccag cgtggcgagc tgacgcgtga tgagcgcaag atccgaggag ctgatccgcc
     1441 ggcgcaggct gaagccggtg cgcgcacggc ggctctcgcg tgcggcggcc gtttcgatcg
     1501 cgagcggaat caggccctgt tcgcgcagct tctggcgcac cgcgcgcgcc gtatcgccct
     1561 cgatgatgcc cttcttttcg cgcccgcgag tgtcgagtac ggtgtattcg aaggcgccca
     1621 tgtcaggaga gaaacgagga aagcatgccg agacgcgaaa gcggagcggc gcgaagcgga
     1681 atgcgacgat tcattgcgaa gctcgcgctt cgcacgtcta taccatcact tctcgatgcg
     1741 tccgcgccga tctcagtctt cacgggtaac ccgcaacact tcctcgacac tcgtttcgcc
     1801 cgcgagtacc cgttcgcgcc cgtgcgcgcg aatgcccgcg gacctggtgc gcgcgtaggc
     1861 ttcgaggtcc tgctcgccgg tgcggtcgtg aatcaggcgg cgcatcttat cgtccacggg
     1921 caccagttcg tagatgccgg tgcgtccgcg ataccccgtg cgattgcagg cctcgcagcc
     1981 cacggcccga tagaacgtga gcgggccttt gtcggcgaat ccgatgagtt tgcgctcgcg
     2041 ctcttcgggc cgatagggct cgcggcagtc cgcgcagagc aggcgcacga ggcgttgggc
     2101 aagcagcccg atgaggctcg aggaaagaag aaacgattcc acccccatgt cgtcgaggcg
     2161 cgggatcgct ccgactgcgg tgttcgtatg cagagtcgag agcaccagat gccccgtgag
     2221 cgaggcctgg atcgcgattt cggccgtttc cagatcgcgg atttctccga ccatgacgac
     2281 atcggggtcc tggcgaagga tcgcgcgcag gccgcgggcg aaggtcatct gcacgcgcgt
     2341 attgacctgg gtctggccga tgccgtcgat gtagtattcg atcggatcct cgaccgtcat
     2401 gatgttgcgg ctgtgatcgt tgagccgcat cagcgccgca taaagggtcg tcgacttgcc
     2461 ggaacccgtc ggcccggtga cgagaagaat gccgtgcggt ttgcggatga ggtcttccag
     2521 gacttcgcgc gtcggcgcgg gcatgcccag gtgctgcaac gaaatggtgc cggcctgctt
     2581 gtcgagcaag cgcatgacca ctcgctcgcc gtgaccgcag ggaatcgtcg agacgcgcac
     2641 gtccaccgga cgaccggcga gacgcagcga aatgcgcccg tcctggggaa ggcgcttctc
     2701 ggcgatgtcg agccgcgcca tgaccttcac tcgcgagacc acgagcgggg cgacggcgcg
     2761 gcgcgatttc agcacttcgc gcagcacgcc gtccacgcgc atgcgcacga cgagatagtc
     2821 ctcgaagggt tcgatatgga tgtcggaggc gttctcgcgc acggcttcgc tgagcaatgc
     2881 gttgatgagg cggatgatcg gtgcatcgtc gtccgcttcg agcagatcct cgggctcggg
     2941 cagctgatcg gcgacgctcg acagatcggt gcgcgcctcg agcgtctcga agccctcgat
     3001 ggtctgatgc tcttcgtaac tttgctggag caggcggtcg aactcctcgc ggtcgatcgc
     3061 ctcgaggcgc agcgggcgcg agaagagccg ccgcaattcc aacagcgcga gcggatcggc
     3121 gtcgcggcga tgcgcgatca gcgcatggtc gtcggcgagt tcgcgcac
//
LOCUS       DASUJG010000076         6273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_754097, whole genome shotgun sequence.
ACCESSION   DASUJG010000076 DASUJG010000000
VERSION     DASUJG010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6273
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754097"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..2185)
                     /locus_tag="VFX38_01470"
     CDS             complement(203..2185)
                     /locus_tag="VFX38_01470"
                     /inference="COORDINATES: protein motif:HMM:NF025670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HEX5313566.1"
                     /translation="MLPVGFEQGFTIAKRPSGTGELTLALAMSPSNAESPFIRHSRAG
                     GNPVQTSPSLPLRSERGKEIAWGKLRYGQLVVTDANGKLLPATFRVAQDRDQARPYRA
                     GERILIAVNDAQAVYPLTVDPLVWLDQKVTASDGADSDAFGWSVALSGATALVGAPFA
                     TGNSPHGAAYAFSESNGTWTQTAKLTASDGAAYDRFGQSVALDGSTALIGAYEANGYQ
                     GAAYVFGESGGNWSQTQELTASDGAAGDQFGYSVALSGNTMLIGSNFATVNGTLWQGA
                     AYVFSESNGSWTQTAKLTASDGAASDNFGSAVALSGATALIGALATVNGNTAQGAAYV
                     FGDSGGSGTQTAKLTASDGAASDDFGNSVALSGAIALIGARLATVNGNQYEGAAYAFG
                     ISGGSWTQTAKLTASDGVAFDDFGASVALSGATALIGADVATVNGNEGQGAAYLEGNT
                     NLDLLSTAPTWVYQNDTYLSQAIVTNEATVTLPAVAVTVTVPAAASFVSASAIPGSCG
                     ESAGLVTCDFGSIPGRGFAVASINLKATGLPGTTIGNTAGIVKAEPALTAGSPTVITA
                     ACPAGYLGYSGLLTPDARDRMSPYQSAAGNQTVLFSASPDFALYVGYQKDAQAKWTIR
                     RISRQAIYHHGPAGTYRWQINAGSDGGAYTLCILHP"
     gene            complement(2226..2561)
                     /locus_tag="VFX38_01475"
     CDS             complement(2226..2561)
                     /locus_tag="VFX38_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313567.1"
                     /translation="MCIQGKLGSGLFVFLLALAGGGATALAAPSGTSTAGAEARPAHS
                     TQALPATLRAVFYQALANDAGAAYAIHEDGCVMLPKQPIKGCFDSTGAHFTGTTPLSH
                     RRKVRDQRQ"
     gene            2932..3519
                     /locus_tag="VFX38_01480"
     CDS             2932..3519
                     /locus_tag="VFX38_01480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5313568.1"
                     /translation="MNDAALAAPLFVVNPPGSEDEAGLIARARRRELGAFEALYRSHV
                     GRIHALCRRLSGSPSLAEDYTQEAFVRAWESLPGFRGESAFGSWLHKIAVNVVLGKQR
                     LALRRSAWIRDDPREEDEEAIAAIAAPGERPDIAMDLERAIAELPPGAREVFVLYDVE
                     GYQHEEIAALTGLAVGTSKAHLHRARRQLRARLTS"
     gene            3516..4043
                     /locus_tag="VFX38_01485"
     CDS             3516..4043
                     /locus_tag="VFX38_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008950995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313569.1"
                     /translation="MTDEIPNETLRAALAALPRELAPERDLWPDIEARIAPRRHFAKP
                     WLAAAALAVVLVTGSIAAAIFGPRTPAPTQSVAANPLRAPSGFDAAQQRLRATTIAAS
                     IRESTQLDPKTQTVLLDNLGLIETSLANIQQALARNPGNASLQSLLYQLYRDEAALVA
                     AAQRVQLQTTTGVAL"
     gene            4040..4981
                     /locus_tag="VFX38_01490"
     CDS             4040..4981
                     /locus_tag="VFX38_01490"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEX5313570.1"
                     /translation="MKLSFVKPVVTGIIAVAVFLALMLALDVLLPARAFAGTSIDQTV
                     AVPANADVHVSNVSGSVEATAWNRKEVHIGGTLGTGSLRLAVEKTATGVDIRVVLPEN
                     SHHVEGSRLVIRLPAASRLEAHTVSADVSASGLTGALDVETVSGNVTLRSRSGDISVK
                     TVSGDIALAGSAAGARIGVQTVSGELTLSGINGELQAGSVSGDVSLRGTNTLTRAQLS
                     TTSGDLHFAAALAGDGNYQFHSVSGDVTLDFPAIPDASFNVSSFSGDIEPAFGPPPQR
                     TSEYGPGEDWNYRSGQGNAQVNVDTLSGDVRLRVSHS"
     gene            5412..>6273
                     /locus_tag="VFX38_01495"
     CDS             5412..>6273
                     /locus_tag="VFX38_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313571.1"
                     /translation="MRRGFTPPRVWIATSALCLALAAGPVLAGPAAGAKEPSATVPEL
                     TSWVGDDGKPDASAWKHAAHFSVDNEIQPGRNSPAPVTTEVAVGYTKTALWLRFTAHD
                     PNPRNITVKYRLHDGFDNNDEYVGMIFSPFNDTQWGYEFFCSTGATEFDEFRQSGNEY
                     SSYDAIWYCKAELTKQGYVVTMEIPFRSLKFPHSDQPQTWRMLFFRNWARKVRHQITQ
                     VKVNYNSNCTLCDAEVVRTATPIRAQGANLQIIPQATIARTDTRTSPSSGLVAGSPQI
                     SGGLDARWAIR"
BASE COUNT         1104 a         2143 c         1967 g         1059 t
ORIGIN      
        1 tatcccggcc taccaataaa cctacggcgt ggcactccga gacgtaggtt gggctgaacc
       61 caagcgaagc ccaacgtcat tgcgcacgat tgcgttgggc ttcactacgt tcagcccaac
      121 ctactggctg agcttcgttc atcacgaact cgcaagcccg ctcgctcctg ccgcggccgg
      181 atgagcatca ctaaagtttt cctcacggat gcaggatgca gagcgtgtag gcaccgccgt
      241 cgctgccggc attgatttgc cagcggtagg tgcccgccgg cccgtggtgg tagatcgcct
      301 ggcgggaaat gcggcggatc gtccatttgg cctgcgcatc cttctgatag cccacgtaca
      361 acgcgaagtc cggcgacgcg ctgaacagca cggtctgatt ccccgccgcc gattgatacg
      421 gcgacatgcg atcacgcgcg tcgggcgtta gcaggccgct gtagccgagg tagcccgcag
      481 ggcaagccgc ggtgatcacc gttgggctgc ccgccgtcag ggcgggctcc gccttcacga
      541 tgcccgcggt attcccgatc gtggtgccgg gaagaccggt cgccttgaga ttgatgctcg
      601 cgactgcaaa accgcgccca gggatggagc cgaagtcgca ggtcacgagg ccggcactct
      661 cgccgcaact ccccggaatg gcgctggcgg agacgaagct cgccgccgcc ggcaccgtaa
      721 ccgtgacggc caccgccggc aaagtcacgg tcgcctcatt ggttacgatt gcctgactaa
      781 gataggtatc gttctgatac acccaagtgg gcgccgtact cagcagatcg aggttggtgt
      841 tgccttccaa gtaagccgca ccctgtcctt cattgccatt gacagtagcc acatccgcgc
      901 cgatgagcgc ggtggcgccc gacagcgcga ccgaggcgcc gaaatcgtca aacgcgacgc
      961 cgtcgctcgc ggtgagcttg gccgtctgcg tccagctccc acccgagatg ccgaatgcgt
     1021 aggcggcgcc ctcgtactga ttgccgttga cagtagccaa gcgcgccccg atcagcgcga
     1081 tcgcgcccga gagcgcgacc gaattgccaa agtcgtcaga cgcggcgccg tcgctggccg
     1141 tgagcttggc cgtctgggtc ccgctcccac ccgagtcgcc gaacacgtag gccgccccct
     1201 gtgcggtatt gccgttgaca gtagcgagcg cgccgatcag cgcggtcgcg cccgaaagcg
     1261 cgaccgcgct gccgaaattg tcagacgcgg cgccgtcgct cgccgtgagc ttggcggtct
     1321 gcgtccagct tccgttcgat tcgctgaaca cgtacgccgc gccctgccac aaggtgccgt
     1381 tgacagtggc gaagttcgag ccgatcagca tcgtgttgcc cgaaagcgca accgagtagc
     1441 cgaactgatc gcccgccgcg ccatcgctcg cggtgagctc ctgcgtctgg ctccagttcc
     1501 cccccgactc gccgaacaca taggccgcgc cttggtatcc attggcttcg tacgcgccga
     1561 tgagggcggt cgaaccgtca agggcaaccg actggccgaa gcggtcgtac gctgcgccgt
     1621 cacttgccgt gagcttggcc gtctgcgtcc aggttccgtt cgattcgctg aacgcgtagg
     1681 ccgcgccatg cgggctattg ccagtagcaa agggcgcgcc gacgagcgcg gtcgcgcccg
     1741 agagcgcgac cgaccagccg aacgcgtcgc tgtccgcgcc gtcgctcgcc gtgaccttct
     1801 ggtccagcca caccagcggg tccaccgtca gcggatagac ggcctgcgcg tcgttcaccg
     1861 caatcaaaat gcgctcgccg gcgcggtagg ggcgagcttg gtcgcgatct tgcgcgacac
     1921 gaaatgtcgc cggcaggagt tttccgttgg catcggtcac gaccagttgc ccataacgca
     1981 gcttgcccca cgcaatttcc tttccccgtt cggaacgaag ggggagcgag ggggatgtct
     2041 ggactggatt cccgcctgcg cgggaatgac ggatgaaggg agattcggcg ttcgacgggc
     2101 tcatcgccag agccagtgtc aattcgccgg ttccgctcgg ccgtttggca atcgtgaagc
     2161 cctgctcgaa gccgaccggc aacacccgcc accattcggt gagattgccg tgcgcataac
     2221 tcgcttcatt ggcgttgatc gcggacctta cggcggtggg acaagggcgt cgtgcccgtg
     2281 aagtgcgcgc cggtgctgtc gaagcagccc ttgatcggct gcttcggcaa catgacacat
     2341 ccgtcttcat ggatggcata ggccgcgccc gcatcgttcg cgagcgcttg atagaacacc
     2401 gcgcgcagtg tcgccggcag cgcctgagtc gagtgagccg ggcgggcttc ggcgcccgcc
     2461 gtggaagtgc cggacggtgc ggccagcgct gtcgcgccgc cgcccgccag ggccaacaaa
     2521 aacacgaaca gtcccgaacc aagttttccc tgaatgcaca tcggatatct ccccgggtgg
     2581 ttgtcgaata atgcgagcgg atcggaaggg gtccgtatct ggcacgccag cgatgtcggc
     2641 gtcgggtctg ccggcggctt gtgcttcttg caggccgttc gccgggggct cgggtcggca
     2701 cgcgaatgcg caccggggac aaaatttacg ccggtccccg tagcccgtca agccttttct
     2761 gacctcgccg aacccgcaag cagagacggc gaagatgccg aacgttttgg aacgccggga
     2821 acgccactac gcggcttatc ccggcctgcg acaggactgc attatggcgt ccgacggcta
     2881 tagtgctgca cgtgaataaa cccttttggc tcggcgggca tccaagggga catgaacgat
     2941 gccgcactcg ccgctccgct gttcgtcgtg aacccgcccg ggtctgagga cgaggcgggg
     3001 ctgatcgccc gggcccggcg gcgcgaactc ggagccttcg aggcgctgta tcgaagtcac
     3061 gtcggtcgca tccacgcgct ttgccggcgc ctcagcggct cgccgtcgct cgccgaagac
     3121 tacacgcagg aggcgttcgt gcgggcctgg gagtcgctgc ccgggtttcg cggcgagagc
     3181 gccttcggct cctggctgca caaaatcgcc gtcaatgtcg ttctcgggaa gcagcgcctt
     3241 gcgctgcgcc gttccgcctg gatccgcgac gatccgcgcg aagaggacga agaggcgatc
     3301 gcggcgattg cggcgccggg ggaacggccg gacatcgcca tggatctcga acgcgccatc
     3361 gcggaactcc cgccgggagc gcgcgaggtg ttcgttcttt atgacgtgga aggctatcaa
     3421 cacgaggaaa tcgcggcgct gacgggactc gccgtcggca cctccaaggc tcaccttcat
     3481 cgggcccgcc gccaattgcg ggcgaggttg acatcatgac cgacgaaatt ccgaacgaaa
     3541 ccctgcgcgc ggcgctcgcc gcgctccccc gcgaactcgc accggagcgc gacctctggc
     3601 ccgacatcga ggcgcgcatc gcgccccggc gccacttcgc aaagccgtgg ctcgccgcgg
     3661 cggcgctcgc ggtcgtgctc gtcaccggtt ccatcgctgc cgcgatcttc ggaccgcgca
     3721 cgccggcgcc gacacagtcc gtcgccgcca atccgttgcg cgcgccctcg ggcttcgatg
     3781 cggcgcagca gcgtttgcgc gcgacgacga tcgcggccag cattcgcgaa agcacgcagc
     3841 tcgatccaaa aactcaaacc gtgcttcttg acaatctcgg cctcatcgag acttcgcttg
     3901 ccaacatcca gcaggcgctc gctcgcaacc ccggcaacgc gagcctgcaa agcctgctct
     3961 atcagctcta ccgcgacgag gcggcactgg tcgccgccgc gcaacgcgtc caactgcaga
     4021 ccaccacagg agtcgcacta tgaaactttc cttcgtcaaa cccgtcgtca cgggaatcat
     4081 cgcggtcgcc gtgtttctcg cgctcatgct tgcgctcgac gttctgctcc ccgcgcgcgc
     4141 cttcgccgga acctcgatcg accagaccgt cgccgtcccg gcgaacgcgg atgtccatgt
     4201 ctccaacgtt tcgggaagcg tcgaggccac cgcctggaat cgcaaggaag tccacatcgg
     4261 cgggactctc ggcaccggga gtctccgcct cgccgtggag aaaacggcga ccggcgtcga
     4321 catccgcgtc gtgcttccgg aaaacagtca ccacgtcgaa ggcagccgac tcgtgattcg
     4381 cttgcccgcc gcgagccgcc tcgaagcgca caccgtgagc gccgatgttt cggcttccgg
     4441 gctcacgggc gcgctcgatg tggaaaccgt gagcggcaac gtgacgcttc gttcccgctc
     4501 gggcgacatc tcggtcaaaa ccgtgagcgg cgacatcgca ctcgcaggtt ccgccgcggg
     4561 cgcgcggatc ggagtccaga ccgtgagcgg cgagctgacg ctctccggaa tcaatggcga
     4621 actccaggca ggaagcgtga gcggcgacgt gagcctgcgc ggcaccaaca cgctcacgcg
     4681 ggcacagctc agcacgacct cgggcgacct gcacttcgcg gccgcgctcg ccggcgacgg
     4741 caactatcag ttccactccg tgagcggcga cgtgacgctc gactttcccg cgattcccga
     4801 cgccagcttc aacgtgtcga gcttcagcgg cgacatcgaa cccgccttcg gtcccccgcc
     4861 gcaacgcacg agcgaatacg gccccggcga agattggaat tatcgctccg gccagggcaa
     4921 tgcccaggtg aacgtggaca cgctgagcgg cgacgtgcgc ctgcgcgtat cgcattcgta
     4981 gcggcggagc gggcggatcc ggggttcgcg gcagaggcgc aaggcgcgga ccggatggcg
     5041 gaacacattg cgtctctccg gccaacccgc cctcgcgccc ctgataagac gtgctgcccg
     5101 aacgagggac ttgcatttcc tggaactact gttatactta gctacaacac cgatcttacc
     5161 tggagactga catggctttt atcggcatcg ttctgaccgg caccctcgtt ttcgcgatgg
     5221 ccgcggcgac cgcttcctgg ttgctgagca ggcgctgagg gctcggcgtg ggatccagcc
     5281 cgaaaatcgc ccggccccgt ctgattccgg atcgggcccg aatcgcgggt ctgcccctcg
     5341 ggcgggcatc cggtgccggc ggcgacccgc cgccttcgcg ccggatcgcg accaggcgcg
     5401 ggcgtaatcg catgcgaaga ggattcacgc ccccaagagt ctggatcgcg acgtccgccc
     5461 tttgcctcgc cctggcagcc ggccccgtgc tggcgggtcc ggcggcggga gcgaaggagc
     5521 cctccgccac cgtccccgaa ctcacgtcct gggtcggaga cgacggcaag ccggacgctt
     5581 cggcctggaa gcatgcggcg catttttcgg tcgacaacga aatccaaccc ggacgcaaca
     5641 gcccggcgcc ggtcacgacc gaggttgccg tcggctatac gaaaacggcg ctgtggctgc
     5701 gtttcaccgc tcacgatccg aatcccagaa acatcaccgt caagtaccgg cttcacgacg
     5761 gcttcgacaa caacgacgaa tacgtcggca tgatcttcag ccccttcaac gacacccaat
     5821 ggggctatga attcttctgc agcacgggcg cgacggaatt cgacgagttc cgccagagcg
     5881 gaaacgaata ttcttcctat gacgccatct ggtattgcaa ggccgagttg acgaagcagg
     5941 gctacgtcgt gacgatggag atccctttcc gctcgctcaa gtttccacac agcgatcagc
     6001 cgcagacctg gcggatgctg ttctttcgca actgggcccg caaggtccgt caccagatca
     6061 cgcaggtaaa ggtcaattac aacagcaact gcacactctg cgacgccgag gtggtgcgca
     6121 ctgcgacacc catccgcgcg cagggcgcga acctgcaaat cattccgcag gcgacgatcg
     6181 cacgcaccga cacccgcacg agtccttctt ccggtctcgt cgcgggcagc ccgcagattt
     6241 ccggcggact cgatgcgcgc tgggcaatcc gcc
//
LOCUS       DASUJG010000077         4913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_877802, whole genome shotgun sequence.
ACCESSION   DASUJG010000077 DASUJG010000000
VERSION     DASUJG010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4913
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877802"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..792
                     /locus_tag="VFX38_01500"
     CDS             199..792
                     /locus_tag="VFX38_01500"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5313572.1"
                     /translation="MPWLIGAALTAIAVAWVDRPLARAIAAVLPPRSVQPNVPDVLAP
                     FVAAVTLASLGFFFIARRRGSPRLAAAALCTALAGPLAYGLKELLKWFFGRITVSWYL
                     IHPQAGGFHWLHGQGFFFGFPSGHMLVATALVTAVASFYPRMWPWGQAALAALAIALL
                     LTSYHFLGDLVAGYLLGRLLAHLLIAAVARSGAVRVP"
     gene            complement(680..1513)
                     /locus_tag="VFX38_01505"
     CDS             complement(680..1513)
                     /locus_tag="VFX38_01505"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEX5313573.1"
                     /translation="MPLIASIPHAGTWLPPGYGARLASEVMRALPMTDWHMDALFDFL
                     PALGVSVIAAEASRFVIDLNRPPAGEKLYPGRYETGLVALRSFDDEAIFAEPPAADEI
                     AWACREIYEPYHRELDALLARRRLDGKVILLDLHSVTPEANRISGPLDADIFLGDRDG
                     KSCASWLTDAVAASYEKAGFRLRRNDPYKGGWITERGGRLAGVEALQIEMNWAVYLDP
                     ARPAEGLHSQRFDAARENLQTLFTELAPLLSARLRQSTDAPATARGDSPRPDLRGSDM
                     R"
     gene            complement(1549..2889)
                     /gene="hutF"
                     /locus_tag="VFX38_01510"
     CDS             complement(1549..2889)
                     /gene="hutF"
                     /locus_tag="VFX38_01510"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050416 - formimidoylglutamate deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0019557 - histidine catabolic process to
                     glutamate and formate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HEX5313574.1"
                     /translation="MAGERRLNFRYVLSPEGLLRDRALRLNAQGTILAIEEGSAPWDG
                     TLALPGMPNAHSHVFQRALAGRGEERRGADSFWSWREAMYRLANRLQPESLYDIARQA
                     YGEMLAAGFTAVAEFHYLHHRRDGARGAEMAEAVLAAARGSGIRLRLLPVLYQRGNFD
                     RGPEPAQRRFVHERLEDFLALLETLAPRHPGLAFHSLRAVDAALLAPALAQARRMLGA
                     EIPVHIHLAEQEREVADCRAATGKSPVELLLETGVVDSHWALVHATRATDGELAALAA
                     TGATVVICPLTEASLGDGIFPGAAFLDAGGRIAVGSDANSRIDAVEELRWLEYGQRLA
                     SRARARLADASGLGAPLWSRLAAGGAAALALPLGAIAPGRMADLVVLDPGFESLLGHD
                     PESWADALVVAGDRGALARVYVAGESVVDHGEWAGRAEIGRRYDATLRSLAGMM"
     gene            2954..4144
                     /gene="hutI"
                     /locus_tag="VFX38_01515"
     CDS             2954..4144
                     /gene="hutI"
                     /locus_tag="VFX38_01515"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEX5313575.1"
                     /translation="MYDLTVHNARLYPCAGTLEEAPERSFAVAGGRIAAIGVGADVPS
                     RERFDAAGAVVLPGFVDCHTHLCYAGDRMDEHAARLAGASYAEIAERGGGIASTVAAL
                     ATASEEELVAQSRPRLAALAREGVSTVEIKSGYGLRTREELKQLRAIRRLAEESPLRV
                     LPTFLALHALPLGMDREDYLHEVLEETLPAVAAEKLADSVDVFCEHIAFGVEEMLQVF
                     HRARELGLACRAHTDQLSNLGATRAAASFGVRSCDHLEYAGEADVAALAASGTVAVLL
                     PGAFYFLRETREPPIAALRAAGVPLAIASDCNPGSSPIASPLTILHLACTLWRLSPAE
                     AILGFTTHAAAALGRSNEIGRLAVGASADFTLWDIPAPEFLAYRLGGIRPRAIYLSGQ
                     RHDA"
     gene            4134..>4913
                     /locus_tag="VFX38_01520"
     CDS             4134..>4913
                     /locus_tag="VFX38_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEX5313576.1"
                     /translation="MTLELDGHSLAIASLAQDAGESQRLGLAPAARRAMEASAAAVAR
                     MLAGGEAVYGINTGFGALASERIAAGDLGALQANLVRSHAAGVGEPLPDPIVRRILLL
                     KANGLANGASGVRPALAEALIALLNADVLPVIPERGSVGASGDLAPLAHLSLALIGEG
                     EATRNGEPLAGAEVMRAAGLQPLGLAPKEGLALLNGTQVSTALAIEGLLRGERLLRSA
                     VVAGALSVEGLAGSYAPFDRRIPEARGQTGQMVVAAGFRALL"
BASE COUNT          731 a         1734 c         1665 g          783 t
ORIGIN      
        1 aagataggca aaggcgtcgc cgtcggcgcg ctcgaaggag agccgcagca cccgcggggt
       61 gagctccgcg cgcgccttga gaacgagagt aagtttttcg tgactcacgg cagacgtgcc
      121 acaattcccg catcgagaca aggcgaaata tttttgcaca cggcggggga cacaggtggc
      181 aatcgggcga aactcgcggt gccatggctc atcggcgccg ccttgacggc catcgccgtc
      241 gcctgggtgg accggccgct cgcgcgcgcg atcgccgccg tcctgccgcc gcggagcgtg
      301 cagccgaacg tccccgacgt tctcgctccg ttcgtcgccg ccgtgacgct cgcaagcctg
      361 ggatttttct tcatcgcgcg ccgccgcggc agcccgcgtc tcgccgccgc ggcgctttgc
      421 accgcgctcg cgggtccact cgcctacggc ctgaaggaac tcctgaagtg gttcttcggc
      481 cgcatcaccg tttcctggta cctgattcac ccgcaggccg gcggctttca ctggctgcac
      541 gggcagggct ttttcttcgg ctttccgtcg ggccacatgc tggtggccac ggcgctggtg
      601 acggcggtcg cctcgtttta tccgcggatg tggccctggg gacaggcggc actcgcggcg
      661 ctcgcgatcg cactcctgct cacctcatat cacttcctcg gagatctggt cgcgggctat
      721 ctcctcgggc ggttgctggc gcatctgttg attgccgcag tcgcgcgctc aggagcggtg
      781 cgagttccgt aaacagggtt tgcaggtttt cgcgggcggc gtcgaagcgt tggctgtgca
      841 ggccttcggc cggtcgcgcg ggatcgagat acactgccca gttcatttcg atctgcagcg
      901 cttccactcc cgcgagccga ccgcctcgct cggtaatcca gccgcccttg tagggatcgt
      961 tgcggcgcag gcgaaacccc gccttttcgt aactcgcggc gacggcatcc gtcagccagg
     1021 acgcgcaact ttttccgtcc cgatcgccga gaaaaatatc cgcatcgagc gggccgctga
     1081 tccggttcgc ctccggcgtg acgctgtgca gatcgagcag gatcactttt ccgtcgagcc
     1141 gccggcgcgc gaggagcgca tcgagttccc ggtgatacgg ttcgtagatt tcgcggcacg
     1201 cccaggcgat ttcgtccgcg gcgggaggct cggcaaagat cgcctcgtcg tcgaagctgc
     1261 gcagcgcaac gaggccggtt tcgtagcggc cgggatagag cttctcgccg gccggcgggc
     1321 gattgagatc gatcacgaag cggcttgcct cggccgcaat cacgctcacg ccgagcgccg
     1381 gaagaaaatc gaagagtgcg tccatgtgcc agtcggtcat cggcagggcg cgcatcacct
     1441 cggatgcgag ccgcgcgccg taaccgggcg gcagccaggt gccggcatgc gggatactcg
     1501 cgatcagcgg caagccctct ccttgcggtt cgcgaacgcg gtacgggttc acatcattcc
     1561 cgcaagactg cgcaaggtcg cgtcgtagcg gcgcccgatc tcggcgcgcc ctgcccattc
     1621 cccgtgatcc acgacggatt cgccggcgac gtagacgcgc gccagggcgc cgcgatcccc
     1681 ggcgacgacg agggcatcgg cccagctttc gggatcgtgg ccgaggagcg actcgaagcc
     1741 cggatcgagc acgaccagat ccgccatccg cccgggcgca atcgcgccca gcggcaaggc
     1801 gagcgccgcg gctccgcccg cggcaagccg actccagagc ggagccccga gaccactcgc
     1861 gtcggcgagg cgcgcacgag cgcgcgaggc gaggcgctgg ccgtattcga gccagcgcag
     1921 ctcctccacc gcatcgatcc gcgaattggc gtccgaaccg acggcgatcc gtccgccggc
     1981 gtcgagaaag gctgcgcccg ggaaaatgcc gtccccgagc gaggcctcgg taagcggaca
     2041 gatgaccacc gtggcgccgg ttgcggcgag cgccgcaagt tcgccatccg tcgcccgggt
     2101 cgcgtgcacg agggcccagt gcgagtccac aacgccggtc tcgagcagca gttcgaccgg
     2161 cgattttccg gtcgcggcgc ggcaatcggc gacctcgcgc tcctgctcgg caaggtgaat
     2221 gtgcaccgga atttcagccc cgagcatgcg cctcgcctgc gcgagcgcgg gcgcgaggag
     2281 cgcggcatcg accgcacgca gcgaatgaaa cgcgagcccc ggatgccgcg gcgccagagt
     2341 ctcgaggagc gcgagaaaat cctcgaggcg ctcatgcacg aaacggcgct gcgcgggctc
     2401 gggtccgcga tcgaagtttc cacgctgata gagaaccggc agcagacgca ggcgaatgcc
     2461 cgacccgcgc gccgcggcga gcacggcctc ggccatctcg gcgccgcgcg ccccgtcgcg
     2521 gcgatgatgc agataatgaa attcggcgac ggccgtgaac cccgcggcca gcatctcgcc
     2581 gtaggcctgg cgggcaatgt cgtagaggct ttcgggctgg agacggttcg cgaggcgata
     2641 catcgcttcg cgccagctcc agaacgaatc ggccccgcgc ctctcctcgc cgcggccggc
     2701 aagcgcgcgc tgaaagacat ggctgtgtgc gttcggcatt cccggcagcg cgagcgttcc
     2761 gtcccagggg gcgctcccct cttcgatggc gaggatcgtg ccctgtgcgt tcaggcgcag
     2821 cgcgcgatcg cggagcagac cctccgggct caggacataa cgaaagttca ggcgccgttc
     2881 gccggccatc cgcgggttcc ctgcgggcga tcgcttgcta tgctgatgca attgtctcat
     2941 tcgccgcggc ggcgtgtacg atctcacggt tcacaacgcg cgtctttacc cctgcgccgg
     3001 aacgctcgag gaagcaccgg agcgaagctt cgcggtcgcg ggagggcgca tcgccgcgat
     3061 cggcgtcggc gccgatgtgc cctcccgcga gcgattcgat gccgccggcg ccgtggtcct
     3121 gccgggcttc gtggattgcc acacccatct ctgctacgcc ggcgatcgca tggacgaaca
     3181 cgcggcgcgt ctcgccggag cgagctacgc cgagatcgcc gagcgcggcg gcggcatcgc
     3241 gagcacggtc gcggcgctcg ccacggcgag cgaagaagaa ctcgtggccc agagtcgccc
     3301 gcgcctcgcg gccctcgccc gcgagggcgt gagcacggtc gaaatcaaga gcggctacgg
     3361 tctcagaacc cgcgaggagc tgaagcagct tcgcgccatt cgccgcctcg ccgaggaaag
     3421 cccgctgcgc gtcctcccca ccttcctcgc tttgcatgcg cttccgctcg gaatggatcg
     3481 cgaggactac ctgcacgagg ttcttgaaga gacgctgcct gcggtcgccg cggagaaact
     3541 tgccgacagc gtggatgttt tctgcgagca catcgccttc ggcgtcgagg aaatgctcca
     3601 ggtcttccat cgcgctcgcg aactcggtct cgcctgtcgc gcccacaccg atcagttgag
     3661 caatctcgga gcgacccgcg ccgcggcctc cttcggcgtg cgctcctgcg atcatctcga
     3721 gtacgccggg gaggcggacg tcgcggcgct cgcggcctcg ggaaccgtcg ccgtgctgct
     3781 ccccggcgcg ttttattttc tgcgcgaaac ccgcgagccg ccgatcgcgg cgctgcgcgc
     3841 cgccggagtg ccgctcgcga tcgcgagcga ttgcaatccc gggagttcgc ccatcgcgag
     3901 cccgctcacg attctccatc tcgcctgcac gctgtggcgc ctgagcccgg ccgaggcgat
     3961 tctcggcttc acgacgcacg cggcggcggc actcgggcga tcgaacgaga tcggtcgcct
     4021 cgcggtcggc gcgagcgccg atttcacgct ctgggacatc ccggcgccgg agtttctcgc
     4081 ctatcggctc ggcggcatcc gcccgcgcgc catctactta tcgggacaac gccatgacgc
     4141 ttgaactcga cggtcactca ctcgccatcg cgagcctcgc ccaagacgcg ggcgaatcgc
     4201 aacgcctcgg gctcgcgccc gcggctcgcc gcgcgatgga ggcctcggcc gcggcggtcg
     4261 cgcggatgct cgcgggcggc gaggccgtct acggcatcaa caccggcttc ggagcgctcg
     4321 cgagcgagcg gatcgcggcc ggcgatctcg gcgcgctgca ggccaatctg gtgcgcagcc
     4381 acgccgccgg agtgggcgag cccctgccgg atccgatcgt gcgccgcatc ctgctgctga
     4441 aggcgaacgg gctcgccaac ggtgcttcgg gcgttcgacc ggccctcgcc gaagccctga
     4501 tcgcgctcct caacgcggac gtgctgccgg taatcccgga gcgcggctcg gtcggcgcct
     4561 cgggcgatct cgcgccgctc gcgcatcttt cgctcgccct gatcggggag ggcgaagcga
     4621 cgcggaacgg cgagccgctc gccggggcgg aggtcatgcg cgcggccgga cttcagcccc
     4681 tcgggctcgc tccgaaggaa ggcctcgcgc tcctgaacgg cacccaggtt tccaccgccc
     4741 tcgcgatcga gggcttgctg cgaggcgagc gcctgctgcg aagcgccgtc gtcgcggggg
     4801 cgctttcggt cgaagggctc gccggttcgt acgctccctt cgatcggcgg attcctgagg
     4861 cccgcgggca gacggggcag atggttgtcg ccgcaggctt ccgcgcactc ctc
//
LOCUS       DASUJG010000078         5342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1367274, whole genome shotgun sequence.
ACCESSION   DASUJG010000078 DASUJG010000000
VERSION     DASUJG010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367274"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1747)
                     /locus_tag="VFX38_01525"
                     /pseudo
     CDS             complement(1..1747)
                     /locus_tag="VFX38_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006889499.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
     gene            complement(1819..2619)
                     /locus_tag="VFX38_01530"
     CDS             complement(1819..2619)
                     /locus_tag="VFX38_01530"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5313577.1"
                     /translation="MSGQYIIGGGASGAARLDVLAATTWPASESFLQAAGLGDAKTLL
                     DAGCGNGAITLRIAAGIGRGAAITGLDRDAGMIRFAREAATSQNLPVKFLRCDLEHEP
                     PPGGGYDLVYARLLLSHLRDPEAALGKLAAALAPGGILAAEDVDFEGHYCRPSCAAFS
                     RYVVWYEAAARLRGADPRLGPRLFALFRAAGITRIRRRSLRPAFREGDGKWMALLTLR
                     AIREAVLGAGIATAAEIAATEIELEAFTRSPDSILSLPRFHQVSGRRP"
     gene            2744..4549
                     /locus_tag="VFX38_01535"
     CDS             2744..4549
                     /locus_tag="VFX38_01535"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX5313578.1"
                     /translation="MNDRTGLNPLPPDAVRRMRAALAAHRRGEVERAEAEYGWLTETF
                     PRFADAWHYYGLLCHQRGESDRALTLLRTAQMLAPDHVIFLLNFGRVLSELGKYSRAV
                     VCLGRALRLAPDNDQALIAYAQAQVEAGRGAEAIDRLERRVQGRPADWRLWAWLGKCR
                     EQSGNYPEAKLAFGEAATLAPAPATAEHLRRVRSALESGETITAELEFRAALEIEPHC
                     AAAYLRLARMAMEAGEFGRMREWAQAALERDERLWKAWALLAARPEEYRDAAFVARLQ
                     EAVRKGGDDAGVAPLHFALGWIFEYRNDYDEAFASYARGNRTQGRHRIYDRGAQEAYT
                     SKLIGALDRNFVERAATIGEANAGAIFICGMPRSGTTLVEAMLASHPQVRAGGEMRFV
                     HDWLRRADALESEGETGPWLRDASDATLRGLAREWVAHLRAAAAGSALVTDKMPGNYH
                     LLGLIHVCLPNAPIVYVRRDPRDSGLSCFTTCFDHGHFFSHALDSIGHYYRLQERLME
                     HWREVLPAGRIIDVEYEALIARPEQHARRLLAALELPWDPACLRFHEARRDVRTASLI
                     QVRRPLYRTSVGRWRRFERHLGPLLEELARPLAER"
     gene            4664..>5342
                     /locus_tag="VFX38_01540"
     CDS             4664..>5342
                     /locus_tag="VFX38_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HEX5313579.1"
                     /translation="MKHANEVAAFVRHHYRHFNAAALVDAARGYRAHLGRGGKMMVTL
                     AGAMSTAEIGLSLAEMIRRDKVQIITTTGANLEEDIFNLVAHDHYLRVPHYRELSAAD
                     ERELLERHMNRVTDTCIPEEEAFRRIEHAILAEWRAADSAGERFFPHEFMYRMIRSGA
                     LERHYQIAPEHSWMVAASEKNLPIVVPGWEDSTLGNIFAAHCMKGDINHVHTVRTGIE
                     YMTMLAGW"
BASE COUNT          859 a         1678 c         1853 g          952 t
ORIGIN      
        1 ccttcgaaag agagttcgtc gtcctgctcg atcgcgtcgt agtccttgtc gttcttgaag
       61 gtcagcgcga ggatgccgaa ggtcgcgaga ttctgccagt ggatgcgggc gaaacttttc
      121 gcgatcaccg ccttgacacc gagatagcgg gggccgagcg cggcgtgttc gcggctcgaa
      181 ccctggccgt agttcttgcc gccgacgatg aaatgggcgt cgtccttgtg ttccagcgcg
      241 cgcccggcgt agtcctcgtc gagctggcgg aaggtgaagc ggctgatttc gggaatgttg
      301 ctccgaaacg gcagcacttc ggcgccggcc ggcatgattt cgtcggtgga aacgtcttcg
      361 ccgaccttga tgagcaccgg tccgcccatc tcgtccggca agggtgcgaa gtcggggagc
      421 ggcttgatgt tcggtccctt ctccagttcc gtctgctcgc cttccttcgc cggcggttcg
      481 atcatcgcgg tattgacgat cggttggtcg ggttcctcga atcgcggaca gggcatgtcg
      541 agcgtgcgcg gatcggtgat cctgccggtg agcgccgagg cggcggcggt ttcggggctg
      601 cacagataca cgctgtcttc cttcgtgccg gagcgaccgg ggaaattgcg cggcacggtg
      661 cgcagactga tgcgcccggt cgccggggcc tggcccatgc cgatgcaacc gttgcagccg
      721 gtctggtgca ggcgcgctcc ggcgtggagc agcggcacga gaagtcccat ctgcgagagg
      781 ttttccagaa tctgacgcga ggtcggattg atatcgaagg aaacttgagc gtgcacctgc
      841 ttccctttca tcatcagcgc ggcgatggcg atgtcgcgca ggcccggatt ggccgaggag
      901 ccgatgtagc tctggtaaat ctccttgccg gcgacctcgg cgacgggcac cacgttgccg
      961 ggactcgtgg gtttcgcgat cagcggctcg agccgcgaca gatcgatcgc gtcgcgttct
     1021 tcgtactccg cgtcgtcgtc ggcgagcagt tcggtgaagt cgccgccgcg ctcttcgcgt
     1081 tcgaggaatc gtttgacctc gccatcggag ggaaacaccg tcgtcgttgc gccgagttcg
     1141 gcgcccatgt tggcgatcac atgccggtcc atcgccgtca gtccttcgag gccggggccg
     1201 tagtactcga tgatcttgcc gactccgccg gagactccgt gacggcgaag catttcgaga
     1261 atcacatcct tcgcgctcac ccactccggc aatttgcctt tgagttcaac gcccatcacc
     1321 ttgggcattt tcaggtagaa gggctcgccc gccattgcga gcgcgacttc gaggccgccg
     1381 gcgtgtgact gtcggagccg acgagcgtct ttcccggttt gccgaagcgc tgctgatggg
     1441 tcgggtggct cacgccgttg ccgggccggc tgtaccagag cccgaacttg cggcaggcgc
     1501 tgcggagaaa cagatgatcg tcggcgttct tgaaatcggt ctgaagaaga ttgtggtcga
     1561 cgtactgcgc ggacagttcg gtcttgaccc gcgggattcc catggcctcg aattcgagca
     1621 tgaccatcgt gccggttgca tcctgcgtca gcgtctggtc gatgcggatg ccgatttcct
     1681 cgcccggcgt catggttcct tcgatcaggt gcgattcgat cagcttctgc gctacgttcc
     1741 tgcccacggc cgttctccgg atcccttccc gctaccttag cgcggggcgc cgtgggaaga
     1801 caacgccgac ttttccccct acggccgccg ccccgagacc tgatggaagc gggggaggct
     1861 caggatcgaa tccggcgagc gggtgaaggc ttcgagttcg atttctgtcg ccgcgatttc
     1921 ggccgcggtc gcgatgcccg cgccgagcac cgcctcgcga atcgcgcgca gggtgagcag
     1981 cgccatccat ttgccgtcgc cttcgcgaaa cgccgggcgc aggcttcgcc ggcggatgcg
     2041 cgtgatcccg gcggcgcgaa agagtgcaaa gagccggggg ccgagacgcg gatcggcgcc
     2101 gcgcagccgc gccgccgctt cgtaccagac gacgtagcga ctgaaggcgg cgcaggacgg
     2161 gcggcagtaa tggccctcga agtccacgtc ttcggcggcg agaatcccgc cgggagcgag
     2221 agccgcggca agtttgccaa gcgccgcctc gggatcgcga agatgggaca gaagcaatcg
     2281 tgcgtagacc agatcgtagc cgccgccggg cggaggctcg tgctcgagat cgcagcgcag
     2341 aaatttgacc gggaggttct gtgatgtcgc cgcctcgcgg gcgaagcgga tcatcccggc
     2401 gtcgcgatcg agacccgtga ttgcggcccc gcgcccgatg ccggccgcga tgcgcagcgt
     2461 gatcgcgccg ttgccgcagc cggcgtcgag gagtgtcttc gcgtcgccga gtcccgcggc
     2521 ctgcagaaag gactcgctcg cgggccaggt ggtggcggcg agcacatcga ggcgcgcggc
     2581 gcccgaggcg ccgccgccga tgatgtactg tccggacatc gtggggtcgc tgttcccgga
     2641 ggaagaggct cgcgcatgat tgtagttccg ccgcaagcga cctttggatt cgcagcgcag
     2701 aggatcgcgg tgacgcagcc ggggcaggct atgatggcgc ggcatgaatg atcgaacggg
     2761 gttgaatccc ctgcccccgg atgccgtgcg gcggatgcgc gcggcgctcg cggcgcaccg
     2821 gcgcggagag gtggaacgcg ccgaagcgga gtatggctgg ctcacggaaa cctttccgcg
     2881 cttcgccgac gcctggcatt actacggcct gctctgtcac cagcggggag aaagcgaccg
     2941 cgcgctcacc ctgctgcgca cggcccagat gctcgcgccc gatcacgtca tttttctgct
     3001 caacttcggc cgcgtgctga gtgagctggg gaagtattcc cgggccgtgg tttgcctggg
     3061 gcgcgccctg cggctcgccc ccgacaacga tcaggccctg atcgcctatg cgcaggcgca
     3121 ggtcgaggcg ggacgcggcg cggaggcgat cgatcggctc gagcggcgcg tgcaggggcg
     3181 gcccgccgat tggcgattgt gggcatggct tggcaagtgc cgcgagcaat ccggaaatta
     3241 ccccgaagcg aagcttgcct tcggcgaagc cgcgacactc gcgccggctc cggcgacggc
     3301 agagcatctg cgtcgcgtac ggtcggcgct cgagtcgggg gagacgatta cggccgagct
     3361 ggaattccgc gccgcgctcg agatcgaacc gcattgcgcg gccgcgtacc tgcgtctcgc
     3421 ccggatggcg atggaagcgg gggaattcgg ccgcatgcgc gagtgggcgc aggccgcgct
     3481 ggagcgcgac gagcgtctgt ggaaggcctg ggcgctgctg gcggcacgtc ccgaagagta
     3541 tcgagacgcg gcattcgtgg cccgcctgca ggaggcggtc cgcaaaggag gagacgacgc
     3601 cggcgtcgca ccgctgcatt tcgcgctcgg ctggatcttc gagtatcgca atgactatga
     3661 cgaagccttc gcttcatacg cgcgcggcaa tcgcacgcag ggcaggcacc ggatctacga
     3721 tcggggagcg caggaggcgt acacaagcaa gctgatcggg gcgctcgacc ggaacttcgt
     3781 ggaacgcgcg gcaacgatcg gcgaagcgaa tgccggagcc atcttcattt gcggcatgcc
     3841 ccgctcggga acgaccctgg tcgaagccat gctcgcgagt cacccgcaag tgagggcggg
     3901 cggcgagatg cgcttcgtac acgactggct gcggcgagcg gatgcgctgg agagcgaggg
     3961 cgagaccggt ccgtggttgc gcgacgcgag cgacgccacc ctgcgcggcc tcgcgcggga
     4021 gtgggtggct catctgcgcg ccgcggcggc gggcagcgcg ctggtcacgg acaagatgcc
     4081 cggaaattat catctgctgg gtttgattca cgtttgcctt ccgaatgctc cgatcgtcta
     4141 cgtgcggcgc gatccgcgcg attcgggact gtcctgcttc accacctgct tcgatcacgg
     4201 ccacttcttc tcccatgccc tggattccat cggccactac tatcggctgc aggaacggtt
     4261 gatggagcac tggcgcgagg tgcttcccgc gggccggatc atcgacgtcg aatacgaggc
     4321 gctgatcgcg cgacccgagc aacatgcgag gcgcttgctc gcggcgctgg agctgccgtg
     4381 ggaccccgcc tgtctgcgtt ttcacgaggc ccggcgcgac gtgcgtacgg cgagtctgat
     4441 tcaggttcgc cggccgctct accggacatc ggtggggcgc tggcggcgct tcgagcgcca
     4501 tctcggacca cttctcgaag aactggcgcg gccgctcgcc gagcgctgag ggtgccgtac
     4561 cgccgcaggc tttatacttc gccgaaccgg agtccacgtc gccgcgatgc gattggggca
     4621 gcgagttgcg gccggcagcg ccgctcacag ggagccgaca gtcatgaagc acgccaacga
     4681 agtcgcggcc ttcgtccgtc atcattaccg ccacttcaac gcggcggcgc tggtggacgc
     4741 ggccaggggc tatcgggcgc atctcggtcg cggcggcaag atgatggtga cgcttgcggg
     4801 cgcgatgagt acggccgaga tcgggctctc gctcgccgag atgatccgcc gcgacaaggt
     4861 gcagatcatt accacgacgg gggcgaatct cgaagaggat attttcaatc tcgtcgcgca
     4921 cgatcattat ctgcgcgtgc cgcattaccg cgagttgtcc gccgccgacg aacgcgagct
     4981 cctcgagcgg cacatgaatc gggtgaccga cacctgcatt ccggaggagg aagcctttcg
     5041 ccgcatcgaa catgcgattc tcgccgagtg gcgggcggcg gactccgcgg gcgagcgctt
     5101 tttcccgcac gaattcatgt accggatgat tcgaagcggc gcattggagc gccactacca
     5161 gatcgcgccg gagcattcct ggatggtcgc ggccagcgag aagaatctgc cgatcgtcgt
     5221 gcccggctgg gaggattcca cgctcggcaa catcttcgcc gcgcattgca tgaagggcga
     5281 catcaaccac gtgcacaccg tgcgcaccgg gatcgaatac atgacgatgc tcgccgggtg
     5341 gt
//
LOCUS       DASUJG010000079         5249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1612055, whole genome shotgun sequence.
ACCESSION   DASUJG010000079 DASUJG010000000
VERSION     DASUJG010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1612055"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /locus_tag="VFX38_01545"
     CDS             <1..1154
                     /locus_tag="VFX38_01545"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017942864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX5313580.1"
                     /translation="LGGKRVLMRVDFNVPLKDGAIANDARIEAALPGIRRLLDSGARL
                     LLMSHLGRPEEGKPEKKYSLEPVARRLGELLGAEVPLARDYLNDAPQPAAGQALLLEN
                     VRFNEGEKKNDAALAKRYAALCDLYVMDAFGSAHRAQASTEGVTRVAPAACAGPLLVA
                     ELEALGRALEKPARPFAAILGGAKAADKLGVIEALVEKCDTLILGGGIANTFLAATGN
                     PVGRSLHDADFLDEARKLIDRARARGVRLPLPSDVVVAEELSEDAEADVRASAEVAPG
                     EMILDIGPETAAIYRRALADAATIVWNGPIGVFEHDQFAQGTRAIAETVAASGAFSLI
                     GGGDTIAALDRFGLRERVSYVSTGGGAFLEFVEGKKLPAVAALEDRARG"
     gene            1168..2610
                     /gene="pyk"
                     /locus_tag="VFX38_01550"
     CDS             1168..2610
                     /gene="pyk"
                     /locus_tag="VFX38_01550"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX5313581.1"
                     /translation="MRRTKIVATLGPATDRDEVLEQVLAAGVDVVRLNFSHGEADEQR
                     RRVAAVRAAAAKLGRDVGVLMDLQGPKIRIECFRDGPVELEEGASFTLDVSLGTQEGD
                     RERVGVTYKKLPQDVHAGDTLVLADGQIALTVDEVDGPRICCRVKSGGTLSDHKGVNR
                     LGGGLSAETLTDKDREDIKIAAECDCDYLAVSFPRSASDVERARRLLRAAGGEASIVA
                     KIERAEAVENLDAIILASDAVMIARGDLAVEIGDAPLPGVQKRIVRRARKYDTAVITA
                     TQMMESMVTSPVPTRAEILDVANAVLDGSDAVMLSEETAVGKYPVKVVEAMARVCIGA
                     ESDLELGAASPEEELHFAQVDQTIAMAAIYVAKHLAVRALVTLTETGATALYMSRVNS
                     EIPVYAFTRHERTRRRVTLYRNVYPVPFAEDPESAKVVHDVAATLVARDAVGAEDAIL
                     LTRGVQSGVSGGTNSLHVVRVADVLGGKRS"
     gene            2706..3077
                     /locus_tag="VFX38_01555"
     CDS             2706..3077
                     /locus_tag="VFX38_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004872196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5313582.1"
                     /translation="MRMRILAVLLVATAGALTTGAFAAGTDVPKMLAEQGCTGCHADK
                     TKVVGPAWGWVAYHYKDTAKQKAVADVAAFIISGGTGYWKKWTGGIPMPAHSNLSRNQ
                     ADAIASWILSQPPIAPPKPGA"
     gene            3230..4837
                     /locus_tag="VFX38_01560"
     CDS             3230..4837
                     /locus_tag="VFX38_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5313583.1"
                     /translation="MIRFFALCASALVAIAPAYAAAPPASAPAAASVTQNYKIPYPVA
                     KVPPDASPALRREIKKGEYLTKISDCMACHTDHDQTSIEGKPFAGGLPITTPFGIIYS
                     RNITPDTETGIGAWTFQQFDDAVRYGKSPQGYLFAAMPYNYYNAMSRADVLAIWEFLK
                     RVPAVHAEDKPLDMMPPFSWRWLQFGWQFLFFRPAKDTFKPDRDHSAEWNRGRFIVEG
                     PEHCGACHTAHNFLGGAAARFARTGSSITGMWAPNISAFATDPHSVQAITEVFTNARG
                     LGGGTLAGPMLDAISNSMHFMTPADMRAVAIYLRSVKSELPPSVSQVPARDVDLKVGA
                     QIYNGHCAACHASGVGGAPRVGNARDWAALEKTPMIILYENVWHGVSLMPPKGGCNDC
                     TAKELSSAMTYMLQQSRTGTKASEAASAKTPVAASAGAPGNVVSLALGEKIYAAHCSA
                     CHASGVAGAPVYSNSADWAPRIAQGLDSLYQHATHGLGAMPPKGGCTDCTESQIESAV
                     DYMVTGSGGKAEVQKALEQSQQQGSSP"
     gene            4834..>5249
                     /locus_tag="VFX38_01565"
     CDS             4834..>5249
                     /locus_tag="VFX38_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313584.1"
                     /translation="MSGDSVLKFFWWLALGGAMAMIGVMLAGQLGAALILRRLARNET
                     EREALVTRNTWLGLSRPAWLLLVCGAVVGAWWPLFRATLFSGLWPVLVAFAVALLVSP
                     LGQGCRGQIAERWRGLWDLFWAAIAFAALVLMGIGIG"
BASE COUNT          879 a         1799 c         1731 g          840 t
ORIGIN      
        1 cgctcggcgg caagcgcgta ctgatgcgcg tggacttcaa cgtgccgctc aaggacggcg
       61 cgatcgcgaa cgatgcccgc atcgaggccg cgcttcccgg catccggcgg ttgctcgact
      121 ccggcgctcg cctgctccta atgtcgcatc tcggacgccc ggaagagggc aagcccgaaa
      181 agaagtactc gctcgaaccg gtcgcccgca ggctcggcga acttctcggc gcggaagtgc
      241 cgctcgcccg cgattatctg aatgatgcgc cgcaacccgc ggccggtcag gcgctgcttc
      301 tcgagaacgt gcgcttcaac gagggcgaga agaagaacga cgcggcgctc gcgaaacgct
      361 acgcggcact ctgcgatctc tacgtgatgg acgccttcgg cagcgcgcat cgcgcccagg
      421 cctcgaccga gggcgtcacg cgcgtcgcgc cggccgcctg cgccgggccg ttgctcgtcg
      481 ccgaactcga ggcgctcggg cgcgcgctcg agaaacccgc ccgccccttc gccgcgatcc
      541 tcggcggcgc caaggccgcc gacaagctcg gcgtgatcga ggcgctcgtc gagaaatgcg
      601 acacgctcat tctcggcggc ggcatcgcca acacctttct cgccgccacc ggcaatccgg
      661 tcggccgctc gcttcacgac gcggactttc tcgacgaggc gcgcaagctc atcgacaggg
      721 cgcgcgcgcg gggagtgcgc ctgccgcttc cgagcgatgt cgtcgtcgcc gaggaactct
      781 cggaagacgc cgaggccgac gtgcgcgcga gcgccgaagt cgcgcccggc gagatgatcc
      841 tcgacatcgg acccgagacc gcggcgatct accgacgcgc actcgccgac gcggcgacca
      901 tcgtctggaa cgggcccatc ggcgtcttcg aacacgacca gttcgcccag ggaacgcgcg
      961 ccatcgccga gacggtcgcg gcgagcggcg ccttctcgct catcggcggc ggcgacacga
     1021 tcgcggcgct cgatcgcttc ggtctgcgcg agcgggtgag ctacgtctcc accggcggcg
     1081 gcgcctttct cgaattcgtc gagggcaaga aacttcccgc cgtcgcggcg ctggaggacc
     1141 gtgcccgcgg ctgagcttcc ccgccggctg cgccgcacca agatcgtcgc gactctcggc
     1201 ccggcgactg atcgcgacga ggtgctcgaa caggttctcg ccgccggcgt ggatgtggtc
     1261 cgtctcaact tttcgcacgg cgaggcggac gagcagcgac gccgcgtcgc ggcggtgcgc
     1321 gcggcggcgg cgaaactcgg ccgcgacgtc ggcgtgctca tggatctaca gggcccgaag
     1381 atccgcatcg agtgctttcg cgacggtccg gtcgaactcg aggagggagc gagcttcacg
     1441 ctcgacgtgt cgctcggaac acaggagggc gatcgcgagc gggtcggtgt cacctacaag
     1501 aagctcccgc aggacgtgca cgccggcgat accctcgttc tcgccgacgg ccagattgcg
     1561 ctcacggtcg acgaagtcga cggtccgcgc atttgctgcc gggtgaagtc gggcggaacc
     1621 ctttccgacc acaagggggt caaccgcctc ggcggcgggc tttccgccga gaccctcacg
     1681 gacaaggacc gcgaagacat caagatcgcg gccgaatgcg attgcgacta cctcgcggtt
     1741 tcctttccgc gctccgcgag cgacgtcgag cgcgcccgcc ggctcttgcg cgcggccggg
     1801 ggcgaggcct cgatcgtcgc caagatcgaa cgcgccgagg cggtcgagaa tctcgatgcg
     1861 atcattctcg cgagcgatgc ggtgatgatt gcccgcggcg atctcgccgt cgaaatcggc
     1921 gacgccccgc ttcccggcgt gcagaagcgg attgtgcgcc gcgcacgcaa atacgacacc
     1981 gccgtcatca ccgcgaccca gatgatggaa tcgatggtga cgagcccggt accgacgcgc
     2041 gccgagatcc tcgacgtcgc caacgcggtg ctcgacggca gcgatgccgt catgctctcg
     2101 gaggaaaccg cggtcggcaa gtacccggtg aaggtggtcg aggcaatggc gcgggtatgc
     2161 atcggcgccg aatcggacct cgaactcggc gccgcgagcc ccgaagagga gcttcatttc
     2221 gcgcaggtgg atcagacgat cgcgatggcc gcgatctacg tcgcgaaaca tctcgccgtg
     2281 cgcgcgctcg tcacgctcac cgaaaccggc gcgaccgcgc tttacatgtc gcgcgtgaac
     2341 tcggagattc cggtgtacgc cttcacccgc cacgaacgca cgcgccggcg tgtcaccctg
     2401 tatcgcaacg tgtatcccgt tcccttcgcc gaggatccgg aatcggccaa ggtggtgcac
     2461 gacgtggccg cgactctcgt tgcgcgcgat gccgtgggcg ccgaggatgc gatcctgctc
     2521 acccgcggcg tgcaaagcgg agtgtcgggc ggcaccaaca gcctccacgt ggtgcgggtc
     2581 gccgacgtgc tcggcggcaa gcgcagctga gggggtttgc aatcgccggg gcgaagtgca
     2641 agactagtgg ctcggcgatt cggcgatggt ttttcgcccg gaatcgcggg ttcaggagaa
     2701 cttcgatgcg gatgcgtatt ctcgccgtcc tgctggtcgc aacggccggc gcgctcacga
     2761 ccggcgcctt cgccgccggc accgacgtgc cgaagatgct tgccgagcag ggatgcaccg
     2821 gctgtcacgc cgacaagacc aaggtcgtcg gtccggcctg gggctgggtc gcgtaccact
     2881 acaaggacac cgcgaaacag aaggccgtcg ccgacgtcgc cgccttcatc atcagcggcg
     2941 gtaccggcta ctggaagaag tggaccggcg gcattcccat gccggcgcac tcgaacctct
     3001 ccaggaatca ggccgacgcg atcgcgagct ggatcctctc gcagccgccg atcgccccgc
     3061 ccaagcccgg cgcttaatgc cgaatcggca cgcgcaaggc ccggccgcgc gcagccggat
     3121 gcttgggcct gttccgtcgt aactcagccg cgtcgcggca atcccgcgca acgacggcaa
     3181 aagcggccgt tataatgagc ccgtaaccgt cccttcggaa tggggaggca tgatccggtt
     3241 cttcgcgctt tgcgcctcgg ctctggtcgc catcgcgccc gcctacgcgg cggcgccgcc
     3301 cgcatcggca ccggcagccg cctccgtcac gcagaattac aagattccct atcccgtcgc
     3361 gaaggtaccg ccggatgcgt cgccggcgct cagacgcgaa atcaaaaagg gcgagtacct
     3421 cacgaaaatt tccgactgca tggcctgcca cacggaccat gaccagacga gcatcgaggg
     3481 caagcctttc gccggcggct tgccgatcac gacccccttc ggaatcattt attcccgcaa
     3541 tatcacgccg gacacggaaa ccggcatcgg tgcctggacc ttccagcaat tcgacgatgc
     3601 cgtgcgttac gggaaaagcc cgcagggcta tctgttcgcg gccatgcctt acaactacta
     3661 caacgccatg agtcgcgccg acgtcctcgc catctgggaa ttcctcaagc gcgtgccggc
     3721 ggtgcacgca gaggacaagc cgctcgacat gatgccgccc ttcagttggc gctggctcca
     3781 gttcggctgg cagttcttgt tcttccggcc cgccaaggac acgttcaagc ccgatcgcga
     3841 tcattccgcc gaatggaacc gcggccgctt cattgtcgaa gggccggagc attgcggcgc
     3901 ctgccatacg gctcacaact ttctcggcgg cgccgccgcg cgctttgcac ggacgggctc
     3961 gtcaataacg ggcatgtggg cgccgaacat ttccgcgttc gcaaccgatc cgcattcggt
     4021 gcaggcgatt accgaagttt tcaccaatgc gcggggactc gggggcggca cgctcgccgg
     4081 cccgatgctc gatgcaatct cgaacagcat gcatttcatg acgcccgcgg acatgcgcgc
     4141 cgtcgccatt tatctccgca gcgtcaagag cgagttgccg ccgagcgtga gccaggtgcc
     4201 tgcccgcgac gtggatctga aggtcggcgc ccagatctat aacggacact gcgcggcctg
     4261 ccacgcaagc ggcgtcggcg gcgcaccgcg ggtcggcaac gccagggact gggccgcgct
     4321 cgagaaaaca ccgatgatca tcctctacga gaacgtctgg cacggcgtga gtctcatgcc
     4381 tcccaagggc ggttgcaacg attgcaccgc gaaggagctg agttcggcga tgacctacat
     4441 gctccagcag agccggaccg gaacgaaagc gagcgaggcg gcgagcgcga agacaccggt
     4501 tgcggcgagc gccggggctc ccggcaatgt ggtcagtctc gcgctcggcg agaagattta
     4561 cgcggcccat tgctcggcct gccatgcgag cggcgtcgcc ggtgctccgg tgtacagcaa
     4621 ttccgccgac tgggcgccgc gcatcgcgca gggactcgac agtctctacc agcatgccac
     4681 gcatggattg ggcgccatgc cgcccaaggg cggctgcacg gattgcacgg aatcccagat
     4741 cgagtcggcg gtggattaca tggtcaccgg cagcggcggc aaggccgaag tgcagaaagc
     4801 cctcgaacaa tcgcagcagc aaggctcttc gccgtgagcg gcgattcggt cctcaagttt
     4861 ttctggtggc tcgccctcgg cggcgccatg gccatgatcg gcgtgatgct cgcggggcag
     4921 ctcggagcgg cactgatcct gcgtcggctc gcccgcaacg aaaccgagcg cgaggcgctc
     4981 gtgacccgca atacctggct cggattgagc cgtcccgcct ggctgctcct cgtctgcggc
     5041 gcggtggtcg gagcgtggtg gccgcttttt cgcgcgaccc tcttctccgg gctgtggccg
     5101 gtgctcgtcg ctttcgccgt cgcgcttctc gtttcgcctc tcggacaagg ctgccgcggg
     5161 cagatcgccg agcgctggcg cgggctttgg gatctcttct gggccgcgat cgccttcgcg
     5221 gcgctggtgc tgatgggcat cggcatcgg
//
LOCUS       DASUJG010000080         2030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_21942, whole genome shotgun sequence.
ACCESSION   DASUJG010000080 DASUJG010000000
VERSION     DASUJG010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21942"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..1311)
                     /locus_tag="VFX38_01570"
     CDS             complement(208..1311)
                     /locus_tag="VFX38_01570"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEX5313585.1"
                     /translation="MAEGVYLGLMSGTSMDGVDAALVRFAAARVELVAARTTPYEAAL
                     RDALGVLAAGGATRDPIDELGRLDRAVGEAFGAAAAALLAEAGVPAKEVQAIGSHGQT
                     IRHRPDYGFSIQIGDAARIARASGITTVADFRRADLAAGGEGAPLAPIFHRAFFADPD
                     ETRGVLNLGGIANLTLLAPARPVVAFDVGPANILLDPLMRETTGEACDRDGRLAATGR
                     VDEALLAALLADPWFARPPPKSSGPEYFNLAWLRRHAGSAALAPADLAATLTELSAAA
                     VALALDAQDVRPARIFGCGGGVHNPELMRRLAARLPATVLSTTAELGVDPDYVEAICF
                     AWLAGETLAGRPGNLPEVTGARRAVVLGATFHP"
     gene            complement(1378..1992)
                     /locus_tag="VFX38_01575"
     CDS             complement(1378..1992)
                     /locus_tag="VFX38_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017925751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1 family glyoxalase III"
                     /protein_id="HEX5313586.1"
                     /translation="MFRSKAGTGANKTLRALVPLAEGFEESEAVIVIDLFRRANIQVV
                     IAGLSAGSVMGGHGIAVVPDASLHAVADQHFELVVLPGGQPGADNLAADPQLAALLRS
                     ALAKGALVGAICAGPLVLARAGLLSGRRVTSFPGALDDIEPADWHFRTRPVVVDGNLV
                     TSRGPGTAIEFALTLIALAAGPQRRRSVEGRLMRMSIFKAKRPS"
BASE COUNT          349 a          717 c          690 g          274 t
ORIGIN      
        1 acgaaccgga cctttccgga ttggtcgcct gggcactcgg cgcgagcggc ggcgtggaat
       61 tcgacaaagg cagtgcctgc ctgctggaac tcatcgatag ccaccgcgct gtgctgcgtg
      121 cactttatcc gctggaggcg atgactcgac tcgcccgccc gggctgaata gcggccttcc
      181 ctgcgttcga cccaagccac cccgccctca gggatgaaaa gtggctccga gcacgacggc
      241 gcggcgggcc cccgtcactt ccggaagatt gcctggacgg cccgcgagcg tctcgcccgc
      301 aagccaggcg aagcagatcg cctcgacata atcgggatcg actcccagct ccgcggtcgt
      361 gctcagcacc gtcgccggca ggcgggcggc aaggcgacgc atcagctccg gattgtgcac
      421 gccgccgccg cagccgaaga tgcgtgcggg acgaacgtcc tgcgcatcga gggcaagcgc
      481 cactgcagcc gcggacaatt ccgtcagggt cgcggcgaga tcggcgggcg cgagagcggc
      541 actccccgca tgccggcgca gccatgcgag attgaaatat tcgggtccac tgcttttcgg
      601 cggcggacgc gcgaaccagg gatcggcgag gagcgccgcg agcagcgcct cgtccacccg
      661 cccggtcgcg gcgaggcgcc cgtcgcgatc gcaggcctcg ccggtcgtct cgcgcatcag
      721 gggatcgagc aggatgttcg ccggcccgac gtcgaaggcg acaaccgggc gtgcgggcgc
      781 gagcagcgtg agattggcga tgccgccgag attgagtacg ccgcgagtct cgtcgggatc
      841 cgcgaagaac gcccgatgaa agatcggcgc gagcggcgcg ccctcgcccc ccgccgcaag
      901 gtcggcacgg cgaaaatccg cgaccgtcgt gatcccgctc gcccgggcga tgcgcgccgc
      961 atccccgatc tggatggaga agccgtaatc gggacgatgg cggatggtct ggccgtggct
     1021 gccgatcgcc tgcacctcct ttgcgggcac gcccgcctca gcgagcaacg ccgccgcggc
     1081 cgcaccgaag gcttcgccca cggcccgatc gaggcgaccg agttcgtcga tcggatcgcg
     1141 ggtcgcacca ccggccgcga gcacaccgag cgcatcgcgg agcgccgctt catagggagt
     1201 ggtgcgcgcg gcgacaagtt cgacccgagc agccgcgaac cggaccaggg cggcatcgac
     1261 gccgtccatg ctggttcccg acatcaaacc gagatacacg ccctcagcca tgttcgcact
     1321 cggattgcgc tgcgatcgac cgtgccgcca gcgcggcgcc gaaaacgctt cttcgaatca
     1381 actcggacgt ttcgccttga aaatactcat gcgcatcagc cgcccctcga cgctgcgccg
     1441 gcgctgcggc cccgcggcca gtgcgatcag agtgagcgcg aactcgatcg ccgttcccgg
     1501 cccgcgcgag gtgacgagat tgccgtccac caccaccggc cgcgtgcgga aatgccagtc
     1561 cgcgggttcg atatcgtcca gcgctccggg gaaactcgtg acccggcgac ccgaaaggag
     1621 accggcgcgc gcaagcacca gcggcccggc gcagattgcg ccgacgagcg cgcccttggc
     1681 gagcgcagag cgcagcaacg cggcgagctg cggatcggcg gcgagattgt cggcgcccgg
     1741 ctgcccgccc ggaagcacca cgagttcgaa atgctgatcg gcgaccgcat ggagcgaagc
     1801 atcgggcacg acggcgatgc cgtggccgcc catcaccgat cccgcgctca gtcccgcgat
     1861 cacgacctgg atgttggcgc ggcggaaaag atcgatgacg atcaccgcct ccgattcctc
     1921 gaagccctcg gcgagaggaa cgagagcccg cagggttttg ttcgcgcccg tgccggcttt
     1981 cgagcggaac accgcggcgg ctttcaggag attcctgcgg gcggcacgcc
//
LOCUS       DASUJG010000081         3834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_878677, whole genome shotgun sequence.
ACCESSION   DASUJG010000081 DASUJG010000000
VERSION     DASUJG010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878677"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..1249)
                     /locus_tag="VFX38_01580"
     CDS             complement(104..1249)
                     /locus_tag="VFX38_01580"
                     /inference="COORDINATES: protein motif:HMM:NF020874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HEX5313587.1"
                     /translation="MGEAISVAICGTRGIPNNYGGFEQFAQHLAVELVARGYEVIVYN
                     PHFHAVSDDLYHGVKIVSKWCPESLLGPFAHYVYDYLCLRDAMRRRSRIVLECGCTSS
                     GPGLLLLRQRGAIVVLNTDGIEWTRAKWGRLAKALIRLSEKTAIRRAAYLVADNPGIA
                     TYLRKEYDEDATVIPYGTTVPESVDVTLLDSYGLRPNGYFLAVARLEPENNIEAILQG
                     FLASGSAKSLCIVGNHHTRYGRYLRTTFGDERIKYLGGIYRQPVLHALRHFSALYFHG
                     HSVGGTNPSLLDAMADGALICAHDNVFNRAVLGDDGLYFSTREEVAESIRREEENLKL
                     RPRLVENNLAKVRSRYSWQTISDAYEALFLRVSAERSARQGTFRRRA"
     gene            1475..2632
                     /locus_tag="VFX38_01585"
     CDS             1475..2632
                     /locus_tag="VFX38_01585"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5313588.1"
                     /translation="MHRSQLATRATTLLAAGFLAIGIIVLPPAGAASAPAKATAATPA
                     SVTHLRALAEKGNVEAQYTLGVAYGRGQQVKQDYTMAAQWFTKAAKQGYAPAQTVLGV
                     AYAHGQGVPQDYGKAAEWYRKAAKQGDASAQELLGAMYYQGQGLPQNYDEAAKLFLAS
                     ANQGYSDAQYNLGLLYLRGRGVPQDYGKAVAWFRKAAAQGNVNAQYNLGVAYASGQGV
                     AANAKEAASWFRKAAEQGDARAQLGLGAAYAGGQGVSQDLPQAVVWFHKSAVQGNANA
                     QYNLGVAYATGQGTTASPTRAVSWFRKAAAQGEPHAQFGLATSYANGSGVPQDYVQAL
                     MWMIVAQHYGLAKSEDVTTAVATVEKQMTKTQIAVAKKSAAQWLASHQGKQ"
     gene            complement(2733..2800)
                     /locus_tag="VFX38_01590"
     tRNA            complement(2733..2800)
                     /locus_tag="VFX38_01590"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2751..2753),aa:Ser,seq:cga)
     gene            2962..>3834
                     /locus_tag="VFX38_01595"
     CDS             2962..>3834
                     /locus_tag="VFX38_01595"
                     /inference="COORDINATES: protein motif:HMM:NF024750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HEX5313589.1"
                     /translation="MHLTKRTIEAITPPATGYAIHWDDATRGLGVRVTMGRAISFIYQ
                     RRINGRTRRLTIGPFPTLGVEAARRKVARLAGEIADGKDPVAEKRKRSAEGVTLQEAL
                     NGYLAARELKARTRRDVAEAMRSLADWRDKPVKALTPTMIEQRHRKLGKHSEARANLT
                     MRYLRAILNYAAAEWADDNGEPLIAYNPVKRLGATKSWFRVERRRTLLKEHELKPWWQ
                     AVKALGDDPAMRHGAEYRDYLITLLLTGLRRTEALSLTWDAVDFKARTLTITDTKNRE
                     PHTLPLPAFLLELLT"
BASE COUNT          771 a         1231 c         1211 g          621 t
ORIGIN      
        1 aacgcgcccg caagctgact tcaaatcggc gcaagttcaa tgtagcacag gagtcggggg
       61 tgctacaccc cagcctttgc gccgcagagg gcgggatggc gaatcaggcg cgtcggcgga
      121 aagtgccctg ccgcgccgac cgctccgcgg agacgcgaag gaacaaggct tcgtaggcgt
      181 cgctgatggt ttgccacgaa tatcgacttc gcaccttggc gagattgttt tccacgagcc
      241 gcgggcgaag tttcaggttt tcttcctccc gtcggatgga ttccgcgacc tcttccctgg
      301 tggaaaagta gagcccgtcg tctcccagaa cggcgcggtt gaagacgttg tcatgcgcgc
      361 agatgagcgc gccgtccgcc atcgcgtcga gaagcgaagg gtttgtcccg cccaccgaat
      421 gcccgtgaaa atacagggcg gagaaatggc gcagggcgtg cagcaccggc tgacggtaga
      481 tgcctccgag atatttgatg cgctcgtcgc caaacgtcgt gcgcagatag cggccgtagc
      541 gtgtgtggtg gttgccgacg atacataggg acttcgcgct ccccgaagcg aggaagcctt
      601 gcagtatcgc ttcgatgttg ttttccggtt cgaggcgtgc gacagcgaga aaatatccgt
      661 tcggccgcag gccataggaa tccagcagcg tcacgtctac tgactccgga acggtggttc
      721 cgtacggaat gaccgtcgcg tcctcgtcgt attcctttcg cagataggtg gcgattccgg
      781 gattgtcggc cacgaggtag gcggctctgc ggatcgcggt cttctcggac agacggatga
      841 gagccttggc aagacgcccc cactttgcac gcgtccactc gatgccgtcg gtgttcaaca
      901 cgacgatcgc accgcgttgg cgcagaagaa gcagccccgg gccggaactc gtgcatccac
      961 actcaaggac gatgcggctg cggcggcgca tggcatcgcg aagacaaaga taatcgtaca
     1021 cataatgggc aaacggcccg agcagggatt ccgggcacca tttgctcacg attttcacgc
     1081 catggtacaa gtcgtcgctt acggcatgga aatgggggtt gtagacgatg acttcgtagc
     1141 cgcgcgccac gagttcgacc gcaagatgct gggcgaactg ctcgaacccg ccgtagttgt
     1201 tcggaatacc gcgggtcccg cagatcgcca cgctgatcgc ctcgcccatc tccggcttct
     1261 tcgatgtgta tgcccgcgat catacaatga acaggccgcg ggcgcagccc gcgatccgcc
     1321 atcgtccgcc tcggcagccc ggcgcgcacg cgcagatcca cctcgcgtcg ctcccgtttc
     1381 gcggcgcacg aagcgacgag cggggcaact cggccgcctt tgcccgcggc agagttcggc
     1441 tatagtggaa attgcccccg catccggacg tcccatgcac cgctcgcagc tcgcaacccg
     1501 ggccacgact cttcttgccg caggattcct cgcgatcgga atcatcgtcc tgccgcctgc
     1561 gggcgcggct tccgcacccg cgaaggcgac ggctgcgacc ccggccagcg tgacacatct
     1621 ccgcgcgctc gccgaaaagg gcaatgtcga ggcccagtac acgctcggtg tcgcctatgg
     1681 ccgcggccaa caagtgaagc aggattacac catggccgca cagtggttca cgaaagcggc
     1741 gaagcagggc tatgcgcccg cgcaaaccgt gctcggcgtc gcctacgcgc acggccaggg
     1801 cgtgccgcag gactacggca aggccgcaga gtggtatcgc aaggccgcga agcagggaga
     1861 tgcgagcgcg caggagctgc tgggggccat gtattaccag ggccagggcc tgccgcagaa
     1921 ttacgacgag gcggccaagc tgtttctggc gtcggcgaac caaggttatt ccgacgcgca
     1981 gtacaacctc ggtttgctgt acctgcgcgg tcgcggcgta ccccaggact atggcaaggc
     2041 ggtggcctgg tttcgcaagg cggcagcgca gggcaatgtc aacgcgcagt acaacctcgg
     2101 cgtcgcctac gccagcggcc agggcgtggc ggcgaatgcg aaggaggccg catcctggtt
     2161 tcgcaaggct gccgaacagg gagacgcgcg cgcccaactc gggctcggcg cggcctatgc
     2221 cggcggccag ggcgtttcgc aggatctccc gcaggcggtc gtctggtttc acaagtccgc
     2281 cgtgcagggg aacgccaacg cgcaatacaa cctcggcgtc gcctatgcga ccgggcaggg
     2341 caccacggcg agcccgacca gggccgtgag ctggttccgc aaggccgccg cccaaggcga
     2401 gccgcatgcg cagttcggac tcgcgacctc ctatgccaac ggcagcggag tgccgcagga
     2461 ctacgtccag gcactcatgt ggatgatcgt cgcccagcac tacggactcg cgaaatccga
     2521 agacgtgacc acggccgtag cgacggtcga gaagcaaatg acgaaaaccc agatcgccgt
     2581 ggcgaaaaaa agcgccgcgc aatggctggc gagtcaccag ggaaaacaat aggagagcgg
     2641 gcagcgcgtt cgttgccggc gcctcgaaac cagatggagc gcgacgagcg caaccgagaa
     2701 ggttgcgtcc cggcggtgtt cctcggttca attggtgggc ggtcagggat tcgaaccctg
     2761 gaccaacgga ttaaaagtcc gattctggca ctctacgccc gcttacgttt gatctacgct
     2821 catctacaca agagccacat aatcaatagc ttgcatggcc gtcacgagtc cgatggttta
     2881 cgctatacta ctctacagac tgcttacaca gtgcttacac ggcgcttaca cggcgcgaca
     2941 tactgattcg gagcaaggat catgcacctc accaaacgca cgattgaagc gatcaccccc
     3001 ccggccacgg gctacgcaat tcactgggac gatgcaaccc gcgggctcgg cgttcgcgtc
     3061 acgatgggac gggccatatc gttcatctac cagcggcgca tcaacggccg tacccggcgc
     3121 ctgaccatcg ggccgtttcc tacgctcggc gtcgaagcgg cacgccgcaa ggtggcaagg
     3181 ctcgcgggcg agattgccga cggtaaagac cccgtggccg agaagcgcaa gcgtagcgcc
     3241 gaaggcgtga cgctacagga agccttgaac ggctaccttg ccgcccgcga actcaaggcg
     3301 cgcacgcggc gcgacgtagc ggaagcgatg cgttcccttg ccgactggcg ggacaagccc
     3361 gtcaaggccc tgacgccgac gatgatcgaa cagcggcatc ggaaactcgg caagcactcc
     3421 gaagcgcggg cgaacctcac catgcgctac ctgcgcgcga tcctgaacta cgccgccgcc
     3481 gagtgggccg acgataacgg cgaaccgctg attgcctaca accccgtgaa gcgcctcggc
     3541 gcaacgaaaa gctggtttcg cgtggagcgg cggcgcacgc tcttgaagga acacgaactc
     3601 aagccgtggt ggcaggccgt caaggcactg ggcgacgatc ccgccatgcg tcacggcgcg
     3661 gagtatcgcg actatctgat aacgctgctc ctgaccggcc tacgccgtac cgaagccttg
     3721 agcctcactt gggacgccgt ggacttcaag gcccgcacgc tcacgatcac ggacaccaag
     3781 aaccgcgagc cgcatacgct gccgctgccc gcgttcctgc tcgaactcct gacc
//
LOCUS       DASUJG010000082         3101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1613015, whole genome shotgun sequence.
ACCESSION   DASUJG010000082 DASUJG010000000
VERSION     DASUJG010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1613015"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="VFX38_01600"
     CDS             complement(<1..913)
                     /locus_tag="VFX38_01600"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HEX5313590.1"
                     /translation="MSNALANAKSPYLRGHAGNPVDWQEWTPTTLARARAEDRPLFVS
                     VGYSACHWCHVMAREAFSDAEVARILNENFVSIKIDREERPDLDAVFQTAHQALTGRG
                     GGWPLSIFLEPEKLTPFFAGTYFPLTPRYGMPGFADLLQRILAFYAEQRAVLGEQGER
                     LRAMLAERLGADAAPAAGALSGAPVRIATANLAAHFDPVHGGFGGAPKFPQSPQLAFL
                     LARGESGGLRRTLETMARGGLNDHVGGGFFRYTVDAAWRVPHFEKMLYDNAQLLALYA
                     EGAVRFDSPEFRATALATAKFMDAELAL"
     gene            1250..2110
                     /gene="lgt"
                     /locus_tag="VFX38_01605"
     CDS             1250..2110
                     /gene="lgt"
                     /locus_tag="VFX38_01605"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007348932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX5313591.1"
                     /translation="MLIYPNIDPVAFRVGPLAVHWYGLMYLVGIFGGWALLVWRTRKP
                     WVRWTTNQVGDIAFYAALGIVIGGRLGYVLFYNFPYYFAHPERIVQVWDGGMSFHGGL
                     LGVLLACGLYAWRQKRHFFDIMDFIAPVVPIGLAAGRLGNFINGELWGKVANLPWAMR
                     LPCADPRFHMYCDGTLYDFADPSHPGYSFPLQPSQLYELLLEGVALFLICFWFSAKPR
                     PRMAVAGVFALAYGVFRFAVEFVRLPDPQLGYLAFGWLTMGQVLSLPLIAVGIILLAL
                     AYRRRAMVPA"
     gene            2107..2901
                     /locus_tag="VFX38_01610"
     CDS             2107..2901
                     /locus_tag="VFX38_01610"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEX5313592.1"
                     /translation="MKAYLELLNRVLTDGVEKPDRTGTGVRSLFGHQMRFDLAAGFPL
                     VTTKRVHVKSIVYELLWFLRGETNVRWLDEHGVTIWDEWADAAGELGPVYGRQWRSWP
                     AAGGETVDQLAGLVANLRRDPHSRRHVVSAWNVGELDCMALAPCHILFQFWVGDGRLS
                     CQLYQRSADVFLGLPFNIASYALLTHLVAAQTDYAPGEFVWSGGDCHLYRNHFAQARL
                     QLAREPLPLPELALKRKPATLFDYDYEDIAIENYRHHPAISAPVAV"
     gene            2904..>3101
                     /locus_tag="VFX38_01615"
     CDS             2904..>3101
                     /locus_tag="VFX38_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013514956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HEX5313593.1"
                     /translation="MQTIGWADFEKVELRAGTIVAAEPFPEARKAAYKLTVDFGEAIG
                     LRRSSAQITEHYASDDLIGRQI"
BASE COUNT          478 a         1016 c         1021 g          586 t
ORIGIN      
        1 ggagtgcgag ttcggcgtcc atgaacttcg cggtggcgag cgcggtggca cgaaattcgg
       61 gggagtcgaa acggaccgca ccttcggcgt agagcgccag caactgtgcg ttgtcgtaga
      121 gcatcttctc gaagtgcggc acgcgccagg cggcatccac ggtgtagcgg aagaagccgc
      181 cgccgacgtg atcgttgagt ccgccgcggg ccatcgtttc cagcgtgcgg cgcaggcccc
      241 cggattcgcc gcgcgcgagc aggaacgcaa gttgcggact ttgcggaaac ttgggcgcgc
      301 cgccgaagcc gccgtggacg ggatcgaagt gggcggcaag attcgccgtg gcgatgcgta
      361 ccggcgcgcc ggagagtgcg ccggccgcgg gcgcggcatc ggcaccgagg cgttcggcga
      421 gcatcgcccg caggcgttcg ccctgttcgc cgagcaccgc gcgctgctcc gcatagaatg
      481 cgaggatgcg ttgcagcaga tcggcgaagc cgggcatgcc gtagcgcggt gtgagcggga
      541 agtaggtgcc ggcgaaaaac ggcgtgagtt tttcgggttc gagaaagatg gaaagcggcc
      601 agccgccgcc gcggccggtg agcgcctggt gcgccgtctg gaacacggcg tcgagatcgg
      661 ggcgctcctc gcgatcgatc ttgatgctga cgaagttctc gttcaggatg cgcgcgacct
      721 cggcatcgct gaaggcctcg cgcgccatca cgtggcacca gtggcaggcg gagtagccga
      781 ccgagacgaa tagcgggcgg tcttcggcgc gggcacgtgc gagcgtcgtc ggcgtccact
      841 cctgccaatc cacgggattg ccggcgtggc cgcgcagata ggggcttttg gcgttggcca
      901 gtgcgttact catacattgc caatccgggc gctcagccgt cccatctaaa cacacaaacg
      961 gggcggtgag caggcgtcgg catcgtgcta aactcgccgc actccgcgcc ggcgcctcgg
     1021 ccgtcgaatc caaatttgtt cggatcaact ctgtcttgtc cccaaggtcg ccgcgacgcg
     1081 gcggcgacgc ccctcgtccc ggccctctcc cctcaggggg agggagtttg gatgattccg
     1141 accccggccc gatgatgctg cccctcgccc ctgccctctc ccctcggggg agtttggaca
     1201 aatccgacca gggccccgga cccttggacc ccgtacgcaa gggtgggccg tgctgattta
     1261 tccgaacatc gatccggtcg cgtttcgcgt cggaccgctc gccgtgcact ggtacggcct
     1321 catgtacctc gtcgggatct tcggcggctg ggcgctgctg gtgtggcgca cgcgcaagcc
     1381 ctgggttcga tggaccacaa accaggtcgg cgatatcgcg ttctacgccg ccctcggcat
     1441 cgtgatcggc gggcgtctcg gttacgtgct cttctacaac ttcccgtatt atttcgccca
     1501 tcccgaacgg atcgttcagg tctgggacgg cggcatgtcg tttcacggcg gactgctcgg
     1561 cgtgctcctc gcctgcggac tctatgcctg gcggcaaaag cggcacttct tcgacatcat
     1621 ggacttcatc gcgccggtag ttccgatcgg ccttgcggcg gggcgactcg gaaacttcat
     1681 caacggcgag ctgtggggca aggtggccaa cctgccctgg gccatgcgcc ttccctgcgc
     1741 cgatccgcgg tttcacatgt attgtgatgg cactctctat gacttcgcgg atcccagcca
     1801 ccccggctac agcttccccc tgcagccctc gcagctttac gaattgttgc tcgaaggcgt
     1861 ggcgctcttc ctcatctgct tctggttctc ggcgaagccg cgtccgcgga tggcggtcgc
     1921 gggcgtgttc gcgctcgcct acggcgtgtt ccgcttcgcc gtcgagttcg tgcgcctgcc
     1981 cgatccgcag ctcggctatc tcgccttcgg ctggctgacg atgggccagg tcctgtcgct
     2041 gccgttgatc gcggtgggga tcattctgct cgcgctcgcc tatcgccgcc gggcgatggt
     2101 gccggcgtga aggcctatct cgaactgctg aatcgggttc tcaccgacgg cgtcgaaaaa
     2161 ccggatcgca ccggcaccgg cgtgcgctcg ctcttcggcc atcagatgcg cttcgatctc
     2221 gccgcgggct ttccgctcgt gacaacgaag cgcgtgcacg tcaagtccat cgtctacgaa
     2281 ttgttgtggt ttcttcgggg agaaaccaat gtccgctggc tcgacgaaca cggcgtcacg
     2341 atctgggacg agtgggccga cgcggccggc gaactcggcc cggtgtacgg acggcagtgg
     2401 cgatcctggc ccgcggcggg cggcgaaacc gtcgatcagc tcgccggctt ggtcgcgaac
     2461 cttcgccgtg atccgcactc gcgccgccac gtcgtcagcg cctggaacgt cggcgagctg
     2521 gattgcatgg cactcgctcc ctgccacatc ctgttccagt tctgggtggg ggacgggcgg
     2581 ctttcctgcc agctttacca gcgcagcgcc gatgtgtttc tcggcctgcc gttcaacatc
     2641 gcctcctacg cgcttctcac gcatctcgtc gccgcgcaaa cggactacgc gccgggcgag
     2701 ttcgtctgga gcggcggcga ctgccatctc taccgtaatc acttcgcgca ggcgcggctg
     2761 cagctcgcgc gcgaaccgtt gccgttgccc gagcttgcgc tcaagagaaa accggcgacg
     2821 ctcttcgatt acgactacga agacatcgcg atcgagaact accgccatca cccggcgatt
     2881 tccgcgccgg tcgccgtctg aagatgcaga cgatcggctg ggcggatttc gaaaaggtcg
     2941 agttgcgtgc aggcacgatc gtcgcggccg agccgtttcc cgaggcgcgg aaggcggcat
     3001 ataaactcac ggtggacttc ggcgaggcaa tcggcctgcg ccgttcctcc gcgcaaatca
     3061 ccgagcacta tgcttccgac gacttgatcg ggcggcagat a
//
LOCUS       DASUJG010000083         2082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_390353, whole genome shotgun sequence.
ACCESSION   DASUJG010000083 DASUJG010000000
VERSION     DASUJG010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390353"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1685
                     /locus_tag="VFX38_01620"
     CDS             117..1685
                     /locus_tag="VFX38_01620"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5313594.1"
                     /translation="MAKRGAAPTSPRIERALRWRLLELLNIYRLLLAAAIFLVGVVPA
                     FARALALDAPRLTMLAGLVYLLFALGLIAALGRRRPRLSLQNALEPLTDLFAAIVIVQ
                     ATGANLGILAVLLAPPVAVAGAGADSRRRALFFASLATLLLLAVVGGRQIGRSLHVLY
                     YTEAALFGIAAFVLALLSHTFARALGASMTLAEHRGQRLRRADEINRLIVSQLRIGVV
                     VLDAAGKPALVNPAARRLLGEPGLATLAEHGEALAAGSTLREIGGATLRTSSVALAAG
                     EDGGAPARLVFVEEADAAARQAQALKLAALGRLGAAIAHQIRNPLGAIAHAAQLLGEA
                     AAPNARETRLIAIIRRQSARLDRLVGDILGFSRPGSGGSRSLRLRAWLEEYLASERER
                     DPARAARLHVTRPEAGLVVHFDPVHLEHIVANLLDNALRHGDSPAGIELACIEAQGRA
                     AIEVRDRGAGLTADVEALFEPFATTHAAGTGLGLYIARELADANGARLSAAARAGGGA
                     CFRLDFAESPQWLE"
     gene            1682..>2082
                     /locus_tag="VFX38_01625"
     CDS             1682..>2082
                     /locus_tag="VFX38_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5313595.1"
                     /translation="MSDTAAPVLVVDDEPDLRDLLTLTLEEMGLASLPAADLAQARRC
                     ITENAPRLVLTDMRLPDGDGLGLVEWMRREAPGTPVAVLTAYGNIETAVAALKAGAFD
                     FLTKPLDLARLRRLVAAAINLAPSASAGPSPL"
BASE COUNT          282 a          797 c          674 g          329 t
ORIGIN      
        1 gcctgatatt cgtgcagatt catggtatag aaagcctggt tttccgttcc cgcggcgacg
       61 cgaaacgcat gaacgagctc gcgagagacc gcctattttg ggccatcggg cgccgaatgg
      121 caaaacgcgg ggccgccccg accagcccac gcatcgagcg ggccctgcgc tggcgcctgc
      181 tcgaactgct caatatctac cgcctgctcc tcgccgccgc aatctttctc gtcggcgtcg
      241 tgccggcctt cgcgcgggcg ctcgcgctcg atgcgccgcg gctcacgatg ctcgcgggcc
      301 tcgtctacct cctcttcgcc ctcgggctca tcgcggcgct cggccggcgc cgtccccggc
      361 tttccctgca gaatgcgctc gaaccgctga ccgatctttt tgccgccatc gtcatcgtgc
      421 aggcgacggg agcgaacctc ggcatcctcg ccgtgctgct cgcgccgccc gtcgcggtcg
      481 cgggagcggg agccgacagc cgccggcgcg cccttttctt cgcctcgctc gcaaccctgc
      541 tcctgctcgc cgtcgtcggc gggcgccaga tcgggcgcag tctgcacgtt ctctactaca
      601 ccgaggccgc gctcttcggc attgccgcct tcgtgctcgc gctgctttcc cacaccttcg
      661 cgagggcgct cggcgcgagc atgaccctcg ccgaacatcg cgggcagcgc ctgcgccgcg
      721 cggacgaaat caatcgcctg atcgtctcgc agcttcgcat cggagtcgtc gttctcgacg
      781 cggcgggcaa gcccgcgctc gtcaacccgg cggcccgccg gctgctcggc gagccgggcc
      841 tcgcgacgct tgccgaacac ggcgaggctc tggcggcggg cagcacgctg cgggaaatcg
      901 gcggcgcgac gctgcgaacg agcagcgtcg ctcttgcggc aggggaagat ggcggcgcgc
      961 cggcccgcct cgtcttcgtc gaggaggcag acgcggccgc aagacaggcg caggccttga
     1021 agctcgcggc gctcgggcga ctcggcgccg ccatcgccca tcagatccgc aaccctctcg
     1081 gtgccatcgc ccatgccgcg caactgcttg gcgaggcggc cgcgccgaac gcacgcgaga
     1141 cccggctcat cgccatcatc cgccgccaga gcgcacgcct cgatcgcctc gtcggcgaca
     1201 tcctcggctt ctcgcgcccc ggatccggtg ggtcccgttc gctgcggctg cgggcctggc
     1261 tcgaagaata tctcgcctcc gaacgcgaac gcgatccggc gcgcgcggcc cgactgcacg
     1321 tcaccaggcc ggaagccggc ctcgtcgtgc acttcgaccc cgtgcatctc gaacacatcg
     1381 tcgccaatct tctcgacaac gcgttgcgcc acggcgactc gcccgcggga atcgaactcg
     1441 cctgcatcga agcgcagggg cgcgcggcga tcgaggtgcg cgatcgcggg gcgggcctga
     1501 cggcggatgt cgaagcgctc ttcgagccct tcgcgaccac ccacgcggcg ggcaccggac
     1561 tcgggttgta tattgcacgc gaattggcgg acgccaacgg cgcgcgcctc agtgccgcgg
     1621 cgcgcgcagg cggcggcgcc tgcttccgtc tcgatttcgc ggagagtccg caatggctgg
     1681 aatgagcgat accgccgcgc cggtgctggt ggtggacgac gaaccggatc tgcgcgatct
     1741 gctcaccctc acgctcgaag agatgggact ggcctcgctt cccgcggccg atctcgcgca
     1801 ggcgcgccgc tgcatcacgg aaaacgcgcc gcggctcgtt ctcaccgaca tgcgccttcc
     1861 cgacggcgac ggcctcggac tcgtcgagtg gatgcgccgg gaggcgcccg ggactccggt
     1921 cgccgtgctc accgcctacg gcaacatcga aaccgccgtc gccgcgctca aggccggcgc
     1981 cttcgacttc ctcaccaagc cgctcgatct cgcgcgcctg cgccgcctcg tcgccgctgc
     2041 gatcaatctt gcgccgagcg cctccgccgg cccgagcccg ct
//
LOCUS       DASUJG010000084         2071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1124114, whole genome shotgun sequence.
ACCESSION   DASUJG010000084 DASUJG010000000
VERSION     DASUJG010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124114"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1876)
                     /locus_tag="VFX38_01630"
     CDS             complement(<1..1876)
                     /locus_tag="VFX38_01630"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX5313596.1"
                     /translation="MSEAPRHDSRWRFWVDRGGTFTDVIGHAPDGRLHATKLLSNDPA
                     RCEDAVIAGIRRLLGVEADTALPKDRIGEVRIGTTVATNALLERTGASVLFVTNRGLG
                     DALAIGDQSRPDIFALDIRRPPPLHSRVATTGLRFDVEGRELAPLDETALRREFAEAR
                     AAGCTACAIALLHAWRFGEQERRIAAIAREAGFEYVVNSREVSPLMRLLPRAGTAVAE
                     AYLAPVLQRYLSRIAASLPEVPLAFMKSDGGLAGPDAFAACDALLSGPAGGVVGAVET
                     ARRAGFPRVIGFDMGGTSTDVCHHAGEWERRREIMIGGLRLAIPMLAVETVAAGGGSI
                     LGFDGGRLTVGPQSAGADPGPACYRRGGPLTITDCNLLLGRIAPDHFPRLFGPNSDEA
                     LDEFVVKKSFAELAQRMRETPETVAAGALAVATENMTAAIRRISVARGHDPADCVLNA
                     FGGAGGQHACALADALGIDTILLHPLAGVLSALGIGLAERSVLLERGLELELPDAYDA
                     AAAAACELACEARERLVADAPDAEEVACEVTAMLRYEGTDTALPIGFGNAGELASRFA
                     AAHRAHFGFTLEGRPLVMAALRIEARARSRPPEWTEVPAGDLPPPLEHRPVYFDGIRV
                     E"
BASE COUNT          361 a          738 c          668 g          304 t
ORIGIN      
        1 tttcgacccg gatgccgtcg aagtacacgg ggcgatgctc cagcggcggc ggcagatctc
       61 ccgcagggac ttccgtccac tccggcggcc gcgagcgcgc ccgcgcctcg atccgaagtg
      121 cggccattac cagcgggcgg ccttcgagcg tgaaaccgaa atgcgcccga tgcgcggcgg
      181 cgaatcgcga ggcaagttcg ccggcgttgc cgaagccgat cggaagcgcc gtatccgttc
      241 cctcgtagcg caacatcgcc gtcacttcgc aggcgacttc ttctgcatcc ggcgcatcgg
      301 ccacaagccg ctcacgcgcc tcgcatgcga gttcgcacgc ggccgccgcc gcggcatcgt
      361 acgcatccgg aagttcgagt tcgagtccgc gttcgagcag cacgctgcgc tccgcgaggc
      421 cgatgccgag cgccgacagg acgccggcga gcggatgcag cagaatcgta tcgatgccga
      481 gcgcatcggc aagcgcgcag gcgtgctgcc cgccggcgcc gccgaatgca ttcagcacgc
      541 agtccgcggg atcgtgcccg cgggcaacgg agatgcgccg gatcgcggcg gtcatgttct
      601 ccgtcgcaac cgcgagcgcg cccgccgcaa cggtctccgg cgtttccctc atgcgttgcg
      661 cgagttcggc gaacgacttc ttcacgacga actcgtcgag cgcttcgtca ctgttcggcc
      721 cgaacagtcg cgggaaatga tcgggcgcga ttctcccgag cagcagattg caatcggtaa
      781 tcgtgagcgg cccgccccgg cgataacagg cgggaccggg atcggcgccg gcggattgag
      841 gcccgaccgt gagacgtccg ccgtcgaaac cgaggatcga accgccgccg gcggcgacgg
      901 tttcgacggc cagcatcggg atcgcgagac gcaggccgcc gatcatgatc tcgcgccgcc
      961 gttcccattc gcccgcgtga tgacagacat cagtggacgt gccgcccatg tcgaagccga
     1021 tcacgcgcgg aaatcccgcg cgccgcgccg tctccaccgc gccgacaacg ccacccgccg
     1081 gaccggacag cagcgcatcg caggccgcaa acgcatcggg tccggcaagt ccgccgtcgg
     1141 atttcatgaa cgcgagcggc acttcgggaa gcgatgcggc aatccgcgac agatatcgtt
     1201 gcagcaccgg cgcgagatac gcttcggcga ccgcggttcc cgcacgcggc agaagccgca
     1261 tgagcggcga cacttcccgc gaattcacga cgtactcgaa acctgcctcc cgcgcgatcg
     1321 cggcgatcct ccgttcctgt tcgccgaaac gccaagcgtg caggagcgcg atcgcgcaag
     1381 cggtgcaacc cgcggcccgc gcttcggcga actcgcggcg cagagccgtc tcgtcgagcg
     1441 gcgcaagttc gcggccttcg acatcgaaac gcaagcccgt cgtcgcgacg cgtgaatgca
     1501 gcggcggcgg acggcggatg tcgagggcga agatgtcggg ccgcgactga tcaccgatcg
     1561 cgagcgcatc gcccaggccg cgattggtca cgaacagcac cgacgcgccc gtccgctcca
     1621 gcagcgcatt ggtcgcgacc gtcgtgccga tgcgcacttc gccgatccga tctttcggca
     1681 aagccgtgtc cgcttccacg ccgagcagac ggcggatgcc cgcgatcacg gcatcctcgc
     1741 agcgcgccgg atcgttcgaa agcagctttg tggcgtgcag ccgtccgtcc ggcgcatggc
     1801 cgatcacgtc ggtaaacgtg ccgccccgat ccacccagaa gcgccaccgc gaatcgtgcc
     1861 ttggggcttc gctcatcgcg ccattatccg gcaattcccg gcaggccggc ataagccaaa
     1921 gggacggtgc cgccatcctt tgcaccgacg accaaggggc ggcggcctgc ttccgccagc
     1981 cggttccaaa cgaccgcgat cctgccgctg gtcatgtata ctttcgctca tgagtagcga
     2041 atacaccgct gttgtcaagc aggacggcga t
//
LOCUS       DASUJG010000085         4900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_633704, whole genome shotgun sequence.
ACCESSION   DASUJG010000085 DASUJG010000000
VERSION     DASUJG010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4900
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633704"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..1269
                     /locus_tag="VFX38_01635"
     CDS             28..1269
                     /locus_tag="VFX38_01635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5313597.1"
                     /translation="MKTEHCDVLVIGAGPSGSISAAILQRAGLEVRVVERERFPRFMI
                     GESLLPKTMEHLDDAGLFEAANAFGFQVKNGARFIRDGAECMIDFSDCFTPGWTWTWQ
                     LPRADFDKLLADEVAKRGVRFDYETSVTGVAFDGRDSITTVKNAAGDESEIGARFLID
                     ASGYGRVLPKALDLVLPSDQPPRSAFFAQVADSARPAGNEGTQITFMVPRQDAWFWVI
                     PFSNGATSLGFTGSAGFFEGFANKDEAAFRRAVREDPHYGERFEGVEYVMAPRFIKAY
                     ASAVKRLCGPGYAITGNSAEFLDPIFSSGVAFATGSGALAAKLAIRELAGETVDWERE
                     YSEHMRSGVRVFRHYVDAWYDGSLQDIFFAPDFNDDIRRQICSVLAGYVWDANNPFVA
                     RYDRATRAVVNVIRARRPADG"
     gene            1412..2302
                     /locus_tag="VFX38_01640"
     CDS             1412..2302
                     /locus_tag="VFX38_01640"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313598.1"
                     /translation="MRILSFLYVRFGRRLSHALLLPIAAYFFLTSPRARRASRRYLAA
                     VEPLTTGAAARDAKPTARNVSRHLFQFATANLDILAAWHDPERIPVRFPQSDVFFAAA
                     RRRQGMLLISAHFGNPEVSRALLRLAPELRINALVYTERAPKSNALFDEISDAYAIRL
                     IPVQTIGPQTAMLLHEKIAAGEVVVIVGDRTPVAAGSPTVEADFLGRPAAFAAGPYIL
                     AHALECPVYLFFCVAEDGGYTAYLEPFAERVHLPRGARDAALRELAARYAARLADYAA
                     RFPLQWYNFYDFWAPPRRSQ"
     gene            2370..2831
                     /locus_tag="VFX38_01645"
     CDS             2370..2831
                     /locus_tag="VFX38_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313599.1"
                     /translation="MVFLAAGIALALLAGCATEPMMQPQPIAVPAGLSVQQVRSAVAK
                     ALIGRGWVLTGSTPNSYDARLTGDDWQANIRTSFDTKQVTIRYLSSHGLHYTPGHKEL
                     ITQADTGVVEPGNEPQYRRVDDAINPHWNIWMRNLEHDIPAQLSLKVYGTQ"
     gene            complement(2879..3664)
                     /locus_tag="VFX38_01650"
     CDS             complement(2879..3664)
                     /locus_tag="VFX38_01650"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX5313600.1"
                     /translation="MERSGADVLVLLAHNDDEYFAALRIGEEVRLGSRVRVAYLTHGS
                     IYGADSEARFAESAAVLAASGIRGEDMLAIGRREDIFDLKLHERAGDAYAALRGALAG
                     ISIDRLYLPAWEGGHPDHDACHLIGVAFARERGLLGQTYELPCYNRLRVMKFPDDGEA
                     ALFTARDRRRALGVLLSARRYRTQRRTFLGLLPGSIARLLLAGRQQYRPVPAARDYSR
                     PPHAGRLFYEKRFGLSFDEFIAHSATFTAPGTGIAGFCAEALE"
     gene            3847..>4900
                     /locus_tag="VFX38_01655"
     CDS             3847..>4900
                     /locus_tag="VFX38_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5313601.1"
                     /translation="MRNTDIAKPEYFHKVVDCQWACPTHTNVPEYIRMIAAGRYTDAF
                     MLNWYSNVFPGILGRTCDRPCEPACRRARVEDEPVAICRLKRVAADLKDDIRPFLPEV
                     PKKKNGKRIALVGAGPASLAVARDLLPLGYEVVLFDESAKGGGMMRSQIPAFRLPVEV
                     LDEEVNLVLDMGVECRFGRRIDSLQALLAEGWDAVFIGTGAPRGRDLDIPGRAEADAN
                     VHIGVDWLASVAFGHTTSVGKHVVVVGGGNTAMDCCRSALRLGGKEVVTTVRSGFEEM
                     KAHPWEKEETLAEDIPILNWHNPNSFVTKNGRLVAMLFEKVRAEYVDGRRKLVPTGER
                     IELACDDALLAIGQDAV"
BASE COUNT          851 a         1683 c         1561 g          805 t
ORIGIN      
        1 cattttccgc cgctggagtg atcaaacttg aaaacggaac attgcgacgt actcgtgatc
       61 ggcgccggcc cttcgggaag catctcggcc gcgatcctcc agcgcgccgg actcgaggtg
      121 cgcgtggtgg aacgcgaacg ctttccgcgc ttcatgatcg gcgagagcct gctcccgaag
      181 acgatggagc atctcgacga tgccgggctc ttcgaggccg cgaacgcctt cggcttccag
      241 gtgaaaaacg gcgcgcgctt catccgcgac ggcgccgaat gcatgattga tttttccgat
      301 tgcttcaccc ccggctggac ctggacctgg cagttgccgc gggccgactt cgacaagctc
      361 ctcgccgacg aggtcgcgaa gcgcggcgtg cgtttcgact acgaaacgag cgtcaccggc
      421 gtcgccttcg acggccgcga ttcgatcacg accgtgaaga acgcagccgg cgacgaaagc
      481 gaaatcggcg cgcgattcct gattgatgcg agcggctacg ggcgcgtgct gccgaaggcg
      541 ctcgatctcg tgcttccttc cgaccagccg ccgcgctcgg ccttcttcgc gcaagtcgcc
      601 gacagcgccc ggccggccgg aaacgaaggc acgcagatca ccttcatggt gccgcggcag
      661 gatgcatggt tctgggtgat ccccttttcc aatggcgcga cctcgctcgg cttcaccgga
      721 agcgccgggt ttttcgaggg attcgcgaac aaggacgagg ccgcattccg ccgcgcggta
      781 cgcgaagatc cgcattacgg cgagcgtttc gaaggggtcg aatacgtcat ggcgccacgc
      841 ttcatcaagg cctatgcgag cgccgtcaag cggctctgcg gcccgggcta cgcgatcacc
      901 ggcaacagcg ccgagtttct cgatccgatt ttttcctccg gcgtcgcctt cgcgaccgga
      961 agcggtgcgc tcgcggccaa actcgcgatc cgcgaacttg ccggtgaaac ggtggactgg
     1021 gagcgcgaat actcggagca catgcgcagc ggcgtgcggg tcttccgcca ctacgtggat
     1081 gcctggtacg acggcagcct ccaggacatc ttctttgccc ccgacttcaa cgacgacatc
     1141 aggcgacaga tctgctcggt cctcgcgggc tacgtctggg acgcgaacaa tcccttcgtc
     1201 gcccgctacg acagggcgac gcgggccgtc gtcaacgtca ttcgcgcacg gcggccggcg
     1261 gacggctgag cctctcgccg gggagccgac cgcggcagca cacgatgaca atcgggccgc
     1321 gagccgggat aatgcgcgca tgaagcgccg gaacaaaagg ccatacccgc ggcattgggc
     1381 ggaattcgag gaacgcggtt cgcttcttgc gatgcggatc ctctctttcc tgtatgtccg
     1441 cttcggccgc cgcctgagcc acgcgctcct cctgccgatc gccgcatatt ttttcctcac
     1501 ctcgccgcgc gcgcgccgcg cgtcgcgccg ctatctcgcc gccgtcgaac cgctcaccac
     1561 cggagccgcc gcccgcgacg cgaaacccac ggcgcgcaac gtttcccggc atctcttcca
     1621 attcgcgacc gcgaatctcg acatcctcgc cgcatggcac gaccccgaac ggattcccgt
     1681 gcgcttcccg cagagcgacg tgtttttcgc ggccgcccgg cgccgacagg gaatgcttct
     1741 catcagcgcg cacttcggca accccgaggt gtcccgcgcg cttctgcgcc tcgcgccgga
     1801 gctgcgcatc aacgcgctcg tctataccga gcgggcgcca aaatcgaacg cgcttttcga
     1861 cgagatcagc gatgcctacg ccattcggct catcccggtg cagacgatcg gcccgcaaac
     1921 cgcgatgctg ctgcacgaga aaatcgcggc cggcgaagtg gtggtgatcg tcggcgatcg
     1981 cacccccgtc gccgccggga gtccgacggt cgaagccgac tttctcgggc ggcccgcggc
     2041 cttcgcggcc ggaccgtaca tcctcgccca tgcgctcgaa tgcccggtgt atctcttctt
     2101 ctgcgtcgcc gaagacggcg gctacaccgc gtatctcgaa cccttcgccg agcgcgtgca
     2161 tcttccccgc ggcgcccgcg acgccgccct gcgggaactc gccgcgcgct acgccgcgag
     2221 gctcgccgac tatgcggcgc gcttcccgct tcagtggtac aacttctacg acttctgggc
     2281 gccgccgcgc agatcgcagt agatcaagcc gccgtgctag catgctcccg gggcatcgac
     2341 tcgcaaaggg gcggatcatg acaaggcgcg tggttttttt ggccgcaggc atcgcgctcg
     2401 cgctcctcgc cggctgcgcg acggaaccga tgatgcagcc gcagccgatc gccgtcccgg
     2461 ccggtctctc cgtgcagcaa gtgaggtccg ctgtcgccaa agcgctcatc ggccgcggct
     2521 gggtccttac cggctcgacc cccaactcct acgacgcccg tctcacgggc gacgattggc
     2581 aggccaacat ccgcaccagc ttcgacacga agcaagtaac catccggtat ctgtcgagcc
     2641 acggcctcca ttacacgccg ggccacaaag agttgattac tcaggcggac accggggtcg
     2701 tggagccggg caatgagccc caataccggc gagtggatga cgccatcaat ccgcattgga
     2761 acatctggat gcgcaacctg gaacatgaca ttccggcgca actttcgctg aaggtctacg
     2821 ggacgcagta ggcgatcgcg tgcgctgcgg tcgccgatga caggggcgcc tcgcgcgctt
     2881 actctaatgc ctcggcgcaa aacccggcga tcccggtgcc gggcgcggtg aaggtcgcgg
     2941 agtgcgcgat gaactcgtcg aacgaaagac cgaaacgctt ctcgtaaaaa agccggcccg
     3001 cgtgcggcgg tcgtgaataa tcgcgcgcgg ccggtaccgg ccggtactgt tggcggcccg
     3061 caagaagcag gcgcgcgatc gaaccgggca gaagtccgag gaaggttcga cgctgcgtgc
     3121 gatagcggcg cgcggaaaga agaaccccga gcgcccggcg ccgatcccgt gccgtaaaaa
     3181 gcgccgcttc cccgtcgtcg ggaaatttca tgacgcggag gcggttgtag caggggagct
     3241 cgtacgtctg cccgagaaga cctcgctcgc gggcaaaggc gactccgatc agatggcagg
     3301 catcgtgatc gggatgcccg ccttcccagg cgggcagata aaggcgatcg atggaaatcc
     3361 ccgcgagcgc gccgcgaagg gccgcatacg catcgcccgc tctctcatgg agtttcagat
     3421 cgaagatgtc ctcgcgccga ccgatcgcga gcatgtcctc gccgcgaatt ccggatgcgg
     3481 cgagcaccgc ggcgctttcc gcaaaacgcg cctcggagtc ggcgccgtag atgctgccgt
     3541 gagtcaggta ggcgacgcgc acccggctgc cgagccggac ttcctcgccg atgcgcagcg
     3601 ccgcgaagta ctcgtcgtcg ttgtgcgcga gcagcaccag cacgtcggct ccggatcttt
     3661 ccatcctcgg gcaattccga agcgattgga tgggtccagt gtagcgaagg agccgcgggc
     3721 gaaacgaggc ccgcgcctcg gcattctccg ccgccgccgg gctcgtccgc tccggacacg
     3781 gccctccccg gccgccgggg gtacaatcat cgggtttgtt ggcggcgacc ccgagcccga
     3841 ctggccgtgc gcaataccga catcgcgaag ccggagtact tccacaaggt ggtggactgc
     3901 caatgggcct gcccgacgca cacgaacgtg ccggaataca tccgcatgat cgcggccggg
     3961 cgctacaccg acgccttcat gctcaactgg tactcgaacg tattcccggg catcctcggg
     4021 cgcacctgcg atcgcccctg cgaaccggcc tgccggcgtg cgcgggtgga ggacgagccg
     4081 gtcgcgatct gccgcctcaa gcgcgtcgcc gccgatctca aggacgacat ccggcccttc
     4141 cttcccgagg tgccgaagaa aaagaacggc aagcggatcg cgctcgtcgg cgcgggaccc
     4201 gcctcgctcg ccgtcgcccg cgatcttctg ccgctcggct acgaagtcgt gctcttcgac
     4261 gaaagcgcca agggcggcgg catgatgcgc agccagattc ccgccttccg cctccccgtc
     4321 gaggtgctcg acgaggaagt gaacctcgtt ctcgacatgg gcgtcgagtg ccgcttcggc
     4381 aggcgcatcg acagcttgca ggcgctgctc gcggagggtt gggacgccgt tttcatcggc
     4441 accggcgcgc cgcgcgggcg cgatctcgac attcccggcc gcgccgaagc ggatgcgaac
     4501 gtgcacatcg gcgtggactg gctcgcgagc gttgccttcg gccatacgac gagcgtcggc
     4561 aaacatgtgg tcgtcgtcgg cggcggcaac accgcaatgg attgctgccg cagtgcgctt
     4621 cgccttggcg gcaaggaagt cgtgaccacc gtgcgcagcg gcttcgagga gatgaaggcc
     4681 catccctggg aaaaggaaga aacgctggcc gaggacattc ccatcctcaa ctggcacaac
     4741 ccgaattcct tcgtgacgaa gaacgggcgc ctcgtcgcga tgctgttcga aaaggtccgc
     4801 gccgagtatg tggacggacg gcgcaaactc gttccgaccg gcgagcggat cgaactcgcc
     4861 tgcgacgacg cgctgctcgc gatcggccag gatgcggtct
//
LOCUS       DASUJG010000086         3057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1369118, whole genome shotgun sequence.
ACCESSION   DASUJG010000086 DASUJG010000000
VERSION     DASUJG010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369118"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /gene="gloB"
                     /locus_tag="VFX38_01660"
     CDS             complement(<1..749)
                     /gene="gloB"
                     /locus_tag="VFX38_01660"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009619114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEX5313602.1"
                     /translation="MLEIEPIAAFRDNYIWALSVSAVAVVVDPGDAAPVERWLVQHGR
                     RLAAILVTHHHHDHIGGLAALKRRWAPRVYGPAAEPIEEVDLAVAEGDRISPAAGFPA
                     FGVLEVPGHTGGHVAYYGAGLLFCGDTMFSAGCGRLFEGTPAQMYRSLSRLAALPAET
                     RVYAAHEYTVDNLRFARSLLPADGALAQALENAVTIRADAQATLPSTIGREREINLFL
                     RVGEPELRAAIAERTGGSLPTHSEVFAALRR"
     gene            872..1522
                     /locus_tag="VFX38_01665"
     CDS             872..1522
                     /locus_tag="VFX38_01665"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5313603.1"
                     /translation="MHWLDNATLAKRATAEARALAPFVGTWGGRLAVQVGTPSVAFAS
                     PDKFARCIVVSRDEAAAVRARAEALPLAEDSVDVLFLIHVLEETRHPAAVLAEAARVL
                     RGEGRVVVVGFRPLPPTLLRQLPRRIFRSPPRALGLFRLRLLLGRAGLAFERAPGLGG
                     GPIVRHLPGQWQQVFAGSYAVIGRKKIHGMTVLRPSWKRVARKRRQAVLSGHGRAG"
     gene            1512..1952
                     /gene="rnhA"
                     /locus_tag="VFX38_01670"
     CDS             1512..1952
                     /gene="rnhA"
                     /locus_tag="VFX38_01670"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEX5313604.1"
                     /translation="MRVEIYTDGACLGNPGPGGWAAVLIAGGRRRELSGGEAATTNNR
                     MELRAAIAGLQALKRPSEIVLYTDSRYVKQGITEWAARWQANGWRTAARRAVENQGLW
                     QELLAAAAGHRVEWKWVRGHAGVPENERADLLAREAALEFAEAR"
     gene            1949..2671
                     /gene="dnaQ"
                     /locus_tag="VFX38_01675"
     CDS             1949..2671
                     /gene="dnaQ"
                     /locus_tag="VFX38_01675"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020561519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEX5313605.1"
                     /translation="MSERLVVLDTETTGLSSDAGHRIIEIACVELENRLRTGRKFHSY
                     LNPDREIEAGARDIHGITDEQLRDQPRFGEIAGELREFISGATLVIHNAPFDVGFLDS
                     EYRRLEPAHASIAAWCKVTDSLAYARNRDRGLANSLDALCRRYHVDNSGRDYHGALLD
                     AQLLVDVWLALTGGQVALALDRREDRGATLAAGIRIGEGLRLPAVRVSEEERAAHRAR
                     LASIRARTGHCLWDKIAGAVCE"
     gene            2716..>3057
                     /locus_tag="VFX38_01680"
     CDS             2716..>3057
                     /locus_tag="VFX38_01680"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX5313606.1"
                     /translation="METASQIRAEHVLLMAAIVLAAGSVLGFVARRLRIPDIVLFLVA
                     GIVLGPGVAGVLDIPATSTLSWVIIIFAASYILFDGGAELELRMLGRLWISLAIIVTV
                     GVIVTAFVTGIA"
BASE COUNT          470 a         1029 c         1052 g          506 t
ORIGIN      
        1 aatcggcgta gtgccgcaaa tacctccgaa tgcgtcggca gggaaccgcc agtcctttcc
       61 gcgatcgccg cgcgcagctc cggctcgccg acccgcaaaa agagattgat ctcacgctcc
      121 cgcccgatcg tcgagggcag cgttgcctgc gcatccgcgc ggattgtaac agcattttcc
      181 agcgcctgtg caagggcgcc gtccgccggg aggagcgagc gcgcgaagcg caggttgtcg
      241 acggtatatt cgtgggccgc atagacccgc gtttccgccg gcaacgccgc cagccggctc
      301 agcgagcggt acatctgcgc gggcgttcct tcgaacagtc gcccgcatcc cgccgagaac
      361 atggtatcgc cacagaagag cagaccggcg ccgtagtacg ccacatgccc gcctgtgtgt
      421 cccggcactt cgagcacccc gaaagccggg aatcccgcag ccgggctgat cctgtcgcct
      481 tcggcgacgg caaggtccac ctcctcgatc ggttccgctg cgggcccgta gacccgcggg
      541 gcccagcgcc gtttgagcgc cgccagcccg ccgatgtggt cgtgatgatg atgggtaacg
      601 aggatggccg cgaggcgtct cccgtgttga acgagccagc gctcgaccgg ggcggcgtcc
      661 ccgggatcga ccacgacggc gaccgcagat accgacaatg cccagatgta attgtcgcga
      721 aaggcggcga tcggttcgat ttcgagcacg cgtcatggta gcagcgctgc ggcggcgaag
      781 aatgcggaga cttgctcggg ccgcgccgtc gcctttctcc gtggccgggc tacaatgcgg
      841 ggacgaccgc gaagaggccg gcacttcccc gatgcactgg ctcgacaacg caacgcttgc
      901 gaaacgggcc acggccgagg cgcgggcact cgcccccttc gtcggcacct ggggcgggcg
      961 ccttgccgtg caggtcggaa cgccctcggt tgccttcgcc tcgcccgaca agtttgcccg
     1021 ctgcatcgtc gtttcccgtg acgaggcggc ggcggtgcgc gcgagggcgg aggcgctgcc
     1081 gctcgccgaa gattcggtcg atgtcctgtt tctgattcat gtgctggagg aaaccaggca
     1141 tccggccgcc gtgctggcgg aggcggcgcg agtgctgcgc ggagaaggcc gcgtggtcgt
     1201 cgtcggattc cgcccgcttc cgcccacgtt gttgcgccag ttgccgcggc ggattttccg
     1261 gtcgccgccg cgggcgctcg gactgtttcg cctgcgtctg ctgctcgggc gtgccggcct
     1321 cgccttcgaa cgcgcgccgg gcctcggcgg cggcccgatt gtccgccacc ttcccgggca
     1381 gtggcagcag gtcttcgccg gcagttacgc ggtgatcggg cgcaagaaga ttcacggcat
     1441 gacggtgctg cgcccgagct ggaagcgcgt ggcgcgcaag cggcgtcagg cggtactttc
     1501 gggtcacggt cgtgcgggtt gagatctaca ccgacggcgc ctgcctcggc aaccccgggc
     1561 cgggcggctg ggcggccgtg ctgatcgcgg gcgggcggcg acgcgaactt tcggggggcg
     1621 aggcggcgac gaccaacaat cgcatggaac ttcgcgccgc cattgccgga ttgcaggcgc
     1681 tgaagcgccc gagcgaaatc gtgctctata ccgattcgcg ctacgtgaaa cagggaatca
     1741 ccgagtgggc ggcacgctgg caggcgaacg ggtggcgcac ggcggcgcgt cgcgcggtcg
     1801 agaaccaggg cctgtggcag gaacttctcg cggcggcagc cgggcatcgc gtcgaatgga
     1861 aatgggtgcg cggccacgcc ggggtgccgg agaacgaacg cgccgatctt ctcgcgcgcg
     1921 aggcggcgct cgaattcgcc gaggcgagat gagcgagcgt ctcgtcgttc tcgacaccga
     1981 aacgaccggc ctcagttccg atgcgggcca ccgcatcatc gagatcgcct gcgtcgaact
     2041 cgagaaccgc ttgcgcaccg gacgcaagtt ccattcctat ctcaatcccg atcgcgagat
     2101 cgaggcgggg gcgcgtgata tccacggcat caccgacgag caattgcgcg atcagccgcg
     2161 cttcggcgaa atcgccggcg aactcaggga attcatcagc ggcgcgacgc tggtgattca
     2221 caacgcgcct ttcgatgtcg gatttctgga ttccgaatac cgccggctcg agcccgcaca
     2281 cgcttcgatc gcggcatggt gcaaggtgac ggattccctc gcctatgccc gcaaccgcga
     2341 tcgggggctc gcgaacagcc tcgatgcgct ctgccgtcga taccacgtgg acaacagcgg
     2401 acgcgactat cacggcgcgc ttctcgatgc ccagttgctc gtcgacgtct ggctcgcact
     2461 caccggcgga caggtggcgc tcgcgctcga ccggcgcgaa gatcggggcg ccacactcgc
     2521 ggcgggcatt cgcatcggtg agggactgcg tctgccggcg gtgcgggtaa gcgaggaaga
     2581 acgcgccgcg caccgcgccc gcctcgcgag cattcgcgcg cggacgggcc attgtctgtg
     2641 ggataaaatc gccggcgccg tctgcgagtg acacgtccct gacgcccgcc gggcacggcg
     2701 gcaacgggag tcccgatgga aaccgcttcg caaatccggg ccgagcacgt gctgctgatg
     2761 gcggcgatcg ttcttgcggc gggctccgtt ctcggcttcg tcgcccgccg cctgcgcatc
     2821 ccggacatcg tgctgtttct cgttgccggc atcgtgctcg gtccgggagt cgccggcgtg
     2881 ctcgacattc ccgcgacctc gaccctctcc tgggtcatca tcattttcgc cgcgagctac
     2941 atcctcttcg acggcggcgc ggaactcgag ctgcggatgc tcgggcggct gtggatttcg
     3001 ctcgcgatca tcgtgacggt gggggtgatc gtcaccgcct tcgtcaccgg catcgcc
//
LOCUS       DASUJG010000087         3261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_268188, whole genome shotgun sequence.
ACCESSION   DASUJG010000087 DASUJG010000000
VERSION     DASUJG010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268188"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VFX38_01685"
     CDS             <1..397
                     /locus_tag="VFX38_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="malonyl-[acyl-carrier protein]
                     O-methyltransferase BioC"
                     /protein_id="HEX5313607.1"
                     /translation="DRALLLFATLGPDTLRELRDAWAEVDDHAHVNRFIDMHDLGDAL
                     IRAGFVEPVMDVEEITLTYDHVRELVRDLKAIGVHNVNAERATGLGGPRRFAAFERAY
                     ERFRSEGRLPATYEVVYGTAWTPLDLPRR"
     gene            394..1074
                     /gene="bioD"
                     /locus_tag="VFX38_01690"
     CDS             394..1074
                     /gene="bioD"
                     /locus_tag="VFX38_01690"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEX5313608.1"
                     /translation="MKGFFVTGTDTDAGKTVVAAGMIHALAARGLRVAAMKPVASGGY
                     ATAAGLRNADAEALIAAANVAAGYEEVNPYCFAPPIAPHIAAAEAGLGIEMEMILRAA
                     RALAARADLLLVEGVGGWRVPLGEDFDVAALAAALDLPVVLVVGMRLGCLNHAILSAE
                     AIAVQGKFAGWIANEIDPAMERREENVATLAAKIRAPCLGRVPHLASPAPATVAGFLR
                     FGGAFDGG"
     gene            1064..2419
                     /locus_tag="VFX38_01695"
     CDS             1064..2419
                     /locus_tag="VFX38_01695"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008734671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEX5313609.1"
                     /translation="MAAEVADWSARDLAVLWHPCTQMKDHEWLPLPPIARGEGAWLYD
                     FEGRRYLDAISSWWVNLFGHGNAFIAGRIAEQAHTLEQVIFAGATHRPAIELAERLVT
                     MAPEGLGRCFYADIGSAAVEVALKMSFHYWRNAGRGEKHRFVALANGYHGETLGALGV
                     CDVDLYKEVYRPLLHEAIMAPSPDAWLAEPGESAEDFARRRFAAMEAILAARQAEIAA
                     VILEPLVQCAGNMRMYHPAYLALLREACDRYGVHLIADEIAVGFGRTGTMFACEQAAI
                     APDFLLLSKGITGGFLPLAAVLTREEIYRAFYDDYRRIERAFLHSHSYTGNPLACAAA
                     NATLDLFAESDVLGANRERAAWLAEEAAALRDHPHVGEVRQRGMILAMELVRRRPREA
                     YPWQERRGLRVYQYGLAQECWLRALGDVVYWMPPYVISREEAAHLGRVTRAAIDEATR
                     D"
     gene            2497..2853
                     /locus_tag="VFX38_01700"
     CDS             2497..2853
                     /locus_tag="VFX38_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX5313610.1"
                     /translation="MANSVGYHEPLAELSRETRSLHQALTSLQEELEAVDWYRQRADA
                     CADAELKEILLHNMREEMEHASMVLEWLRRNSADFSGHLRTYLFTEAPILAIEEADTG
                     ADADGDTGKDFTIGKP"
     gene            2959..>3261
                     /locus_tag="VFX38_01705"
     CDS             2959..>3261
                     /locus_tag="VFX38_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HEX5313611.1"
                     /translation="MDYLNRHLAPFSEETWNRIEAAAVAAAREDLTARRFLDVDGPYG
                     VGLTTLELGAEDYCRQPREGEAGAVISSTRALPVPMIRKSFRLSMRRVVAAEEQGAP"
BASE COUNT          550 a         1041 c         1102 g          568 t
ORIGIN      
        1 ggatcgggcg cttctcctct tcgcgacact cggccccgat actctgcggg aactccgcga
       61 cgcctgggcg gaagtggacg accacgcgca cgtaaatcgc ttcatcgaca tgcacgatct
      121 gggcgatgcc ctcattcgcg cgggcttcgt cgaaccggtg atggatgtcg aagagatcac
      181 gctcacctat gatcacgtgc gcgaactcgt ccgcgacctc aaggcgatcg gcgtgcataa
      241 cgtgaacgcg gagcgcgcca ccggcctcgg cggcccgcgg cggttcgcgg ctttcgagcg
      301 cgcctacgag cgtttccgca gcgaagggcg ccttccggcg acctacgaag tcgtgtacgg
      361 taccgcgtgg acaccgctcg atctgccgcg ccgatgaagg gattcttcgt taccggaacc
      421 gataccgacg ccggcaagac ggtcgtcgcc gcgggcatga tccacgcgct cgcggctcgc
      481 ggactgcggg tcgcggcgat gaagcccgtc gcgagcggcg gatacgcgac ggcggcgggt
      541 ctgcgcaacg ccgatgcgga ggcgctgatc gcggcggcga atgtcgcggc cggatacgaa
      601 gaagtgaatc cctattgttt cgcgccgccg attgcacccc acatcgccgc cgcggaggcc
      661 ggcctcggga tcgagatgga aatgattctc cgcgcagcgc gcgctctcgc ggcccgcgcc
      721 gatctcctcc tcgtcgaggg cgtcggcggc tggcgcgtgc cgctcgggga ggatttcgac
      781 gtcgccgcgc ttgccgccgc gcttgatctt cccgtcgtgc tcgtcgtcgg catgcggctc
      841 ggctgcctca atcacgccat cctcagtgcc gaggcgatag ctgtgcaggg aaaattcgcc
      901 ggctggatcg cgaacgagat cgatcctgcg atggagcggc gcgaggagaa tgtcgcaacg
      961 ctcgcggcga aaatccgcgc accctgtctc gggcgcgttc cgcatctcgc gagccccgcg
     1021 ccggcgacgg tggccggatt cctgcggttt ggcggagcgt tcgatggcgg ctgaggtcgc
     1081 ggattggagc gcgcgcgatc tcgcggtgct ctggcatccc tgcacgcaga tgaaggatca
     1141 cgagtggctg ccgcttccgc cgatcgcgcg cggcgaaggc gcctggcttt acgacttcga
     1201 ggggcggcgc tatctcgatg cgatcagctc ctggtgggtg aatcttttcg gtcatggcaa
     1261 tgcctttatc gccgggcgca tcgccgagca ggcgcatacg ctggagcagg tgatcttcgc
     1321 cggcgcgacc catcgtcccg cgatcgaact tgcggaacgc ctcgtcacca tggcgccgga
     1381 aggactcggg cgttgttttt acgccgacat cggctcggcc gccgtcgagg tggcgctcaa
     1441 gatgagcttt cattactggc gcaacgcggg acggggagag aaacatcgtt tcgtcgcgct
     1501 tgccaatggc taccacggcg agacgctggg cgcgctcgga gtttgcgacg tggatctcta
     1561 caaggaagta tatcggccgc tgttgcatga ggcgatcatg gcgccttcgc ccgacgcctg
     1621 gctggccgaa ccgggtgaga gcgcggagga ctttgcgcgc cgccggtttg cggcgatgga
     1681 ggcgatcctc gccgcgcgtc aggcggaaat cgcggccgtg atcctagaac cgctggtgca
     1741 atgcgcgggc aacatgcgca tgtatcatcc ggcttatctc gcgctgctgc gcgaagcctg
     1801 cgatcgctac ggagtgcacc tgatcgccga tgaaatcgcc gtcggcttcg ggcgcacggg
     1861 tacgatgttc gcctgcgaac aggccgcgat cgcacccgat ttcctgcttt tgtccaaggg
     1921 gattaccggc ggtttcctgc cgctcgcggc ggtgctgacc cgcgaggaaa tctaccgcgc
     1981 cttttacgac gactatcggc ggatcgagcg cgcgtttctg cattcgcaca gctacaccgg
     2041 caatccgctt gcctgcgcgg ccgcgaacgc gactctcgat ctgttcgccg agagtgacgt
     2101 gctcggcgcc aaccgcgagc gtgccgcgtg gctggcggaa gaagcggcgg cgctgcgcga
     2161 tcatccccat gtcggcgaag tgcgccagcg cgggatgatc ctcgcgatgg aacttgtccg
     2221 gcgccgaccg cgcgaagcct atccctggca ggagcgccgc ggcttgcgcg tctaccaata
     2281 cggtctcgcg caggaatgct ggttgcgggc gctcggagac gtcgtctatt ggatgccccc
     2341 ctacgtgatc tcccgcgagg aggcggctca tctcggccgc gttactcgcg cggccatcga
     2401 cgaggccacc cgcgactgac gctccgcgcc cgcttgatgc aaatctggac tcagtctaag
     2461 atagcgggaa gcctcaataa tgacgcggag ccgccgatgg cgaacagtgt cgggtatcac
     2521 gagcctctcg cggaactgtc gcgcgagacc cgcagcctcc atcaggcgct gacctcgctg
     2581 caggaggaac tcgaggcggt ggactggtat cgccagcgtg ccgatgcctg cgccgatgcg
     2641 gaactgaagg aaatcctgct gcacaacatg cgcgaagaga tggagcatgc ctcgatggtg
     2701 ctcgaatggc tgcggcgcaa cagtgcggat ttctccgggc atcttcgaac ctatcttttt
     2761 accgaggcgc cgattctcgc gattgaagaa gcggacacgg gcgccgacgc agatggcgac
     2821 acgggcaagg atttcacgat cggcaaaccc tgagcaggca aggtgcagtc gcggcgacgc
     2881 acggttggtg gcgtccgtca ggaacgatat cggaaccgca ggcgcaaggc gaaaaaactt
     2941 tcgacgagga gaacggcaat ggattatctc aatcggcatc tggctccctt ttccgaggaa
     3001 acctggaacc gcatcgaggc ggcggcggtc gcggcggcac gcgaggatct caccgcgcga
     3061 cgattcctcg acgtggacgg accctacggc gtcggcctca cgaccctcga actcggcgcc
     3121 gaagattatt gtcgccaacc gcgcgagggc gaagcgggcg ccgtgatcag ttccacgcgt
     3181 gcgcttcccg tgccgatgat ccgcaagagt ttccggctca gcatgcgccg cgtggtggcg
     3241 gccgaggaac agggagcgcc g
//
LOCUS       DASUJG010000088         4245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1492251, whole genome shotgun sequence.
ACCESSION   DASUJG010000088 DASUJG010000000
VERSION     DASUJG010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492251"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..879
                     /locus_tag="VFX38_01710"
     CDS             172..879
                     /locus_tag="VFX38_01710"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX5313612.1"
                     /translation="MARLPRFVLPGQPQHVIQRGNNREEIFCAEADYRFYLGKLAAAV
                     MRHDCEVHAYVLMTNHVHLLMTPHAEPGIGKALQMLGHYYVQYYNHCYRRTGTLWEGR
                     YRATLIDGEAYLLTCMRYIELNPCGRGWRPARRSIRGRATGAMRWVNPMACSHCTAST
                     GDWTGARRSDRRLIGNYFEPGSARRAWPRYAKRPRRPRCWATTASNAGSNSNCSVGLP
                     LGRGAGSTNRRNIVNQP"
     gene            1174..1626
                     /gene="mraZ"
                     /locus_tag="VFX38_01715"
     CDS             1174..1626
                     /gene="mraZ"
                     /locus_tag="VFX38_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX5313613.1"
                     /translation="MFRGLNQVRLDGKGRLAIPIRYRAELSDVAAGALVATVDRDRCL
                     LLYPRPRWEEIEAKLVELPSLQPEARAVQRMLLGNATELEMDGHGRVLLPTHLRSYAE
                     LDHKVVMIGQGHRFELWSETRWEESARQWAAGIGMSEQGSDALARLSL"
     gene            1645..2523
                     /gene="rsmH"
                     /locus_tag="VFX38_01720"
     CDS             1645..2523
                     /gene="rsmH"
                     /locus_tag="VFX38_01720"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX5313614.1"
                     /translation="MHVPVLLAETLEWLAVRPGGIYVDATYGRGGHSAAILARLDSTG
                     RLYSFDRDPQACADAARFAADGRFEFVRAPFSRLGGILESRGLAGRVDGLLFDLGLSS
                     PQLDDPHRGFGIRHDGPLDMRMDPEAGPSAGKWLARASEREIADVIFRFGEERYSRRI
                     ARAIVARRRAAPIETTGELAALVARAVPRRERRLHPATRSFQALRIHVNDELGELRAG
                     LAAAAMLLAPQARLLVISFQSLEDRIVKQFLREGARSDPPQWKILARLLRPDREELAR
                     NPRARSARLRVAERSA"
     gene            2520..2798
                     /gene="ftsL"
                     /locus_tag="VFX38_01725"
     CDS             2520..2798
                     /gene="ftsL"
                     /locus_tag="VFX38_01725"
                     /inference="COORDINATES: protein motif:HMM:NF016860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEX5313615.1"
                     /translation="MRLRGGPSLALIALAAIVVFGNALAVVYSAARNRALFSELNSLR
                     DHREELMANRGRLELEEWTLAAHSRVADVATKKLHMQPPATVHIVEVP"
     gene            2795..>4245
                     /locus_tag="VFX38_01730"
     CDS             2795..>4245
                     /locus_tag="VFX38_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX5313616.1"
                     /translation="MNPVAARRTLAVAVLFALAALLLIARAVDLQVIRHPLLADRASR
                     QQTRSMELPAHRGMILDRRGQPLAISTPVAAIWVNPKVVSGSGESLAPLAKLLGLDAA
                     RLEADVKAHATRSFLYVERGLPPTTAEQALALGLAGVHSRREYRRYYPSGPVAAPVIG
                     FTNIDDSGQYGLELEYDEWLQGKPGAMRVVTSANGRPVEDDEVIAEPRAGRDLVTSLD
                     MRVQYLAYEALAEAVARQQAVSGSVVVLDVKTGGVLAMANQPSYNPNVRSDLKAALYR
                     NRAVTDLFEPGSSFKPFIIAAALASGGWRPLSTVDTGNGTWTVGGYTIHDDESLGIIT
                     LTTLLEKSSNVGAAKVALSLPAAYLYHVLTGFGFGRPVAGGFPGSVSGELSPYSDWRR
                     VEQAAISRGYGVSVTALELAQGYLAIADGGVFRPVTFLHRDESPTGTRVIPAAIAAEL
                     RAMLTTVVSDEGTGRRAQIANYRVAGKTGTAHI"
BASE COUNT          703 a         1357 c         1426 g          759 t
ORIGIN      
        1 atgtggcgct gatcagggca cttgcgcaag gcattgatcg tgagactgag ggaagtacgg
       61 tatctggccc tgggcgggta tttcttgggc gaagctggat attctgcagg taagtcctcc
      121 agaaatagcg ggaccggagt aagtttggct aaagcttcgc ggggatagca catggcgcgt
      181 ctgccgaggt tcgtcctccc cggtcagcca cagcatgtga tccagcgcgg caacaatcgc
      241 gaagagatat tctgcgccga agccgattac cgcttctact tggggaagct cgcggccgcg
      301 gtgatgcgcc atgactgcga ggtccacgcc tatgtgctga tgaccaatca cgtccatttg
      361 ttgatgacgc cgcacgcaga accgggcatc ggcaaggcgc tgcagatgct cgggcactac
      421 tacgtgcagt attacaacca ttgctatcgg cgcaccggta ccctgtggga ggggcgttac
      481 cgggcgaccc tgatcgacgg cgaggcgtat ctgctgacct gcatgcgtta tatcgaactc
      541 aatccgtgcg ggcggggatg gcggcccgcc aggcggagta tccgtggtcg agctaccggg
      601 gcaatgcgct gggtcaaccc catggcttgc tcacattgca ccgcgagtac cggagactgg
      661 accggagccc ggaggagcga caggcggctt atcggcaatt atttcgagcc tgggtcagcg
      721 agaagagcct ggccgagata cgcaaagcga ccaagaaggc ctcggtgctg ggcgacgacc
      781 gcttcaaacg ccggatcgaa cagcaattgc agcgtagggc tgcccctcgg gcgcggggcg
      841 gggagcacaa atcggcggaa tatcgtcaac cagccgtaat caatcgagtc tgacctcatt
      901 gaagaagccg cgacgcggcg atttgcgcag ggccgcgctt tcatcgttgt ggcgatcttt
      961 cgcgcgaaaa gccacccgtc ctgtcggttc gccggaatat tcggcggcaa gttgctgaat
     1021 ctttgaactt cggcaggacg tgaatccgct gccgtcgcca aaaacgcccc ggcaaggccg
     1081 tttcacgctt gaatcccctt gacttttcaa aaatttggct cctatagtgt agctccgtgg
     1141 gatggagtgg gagtgagtgg gaggagcggt ctgatgtttc gtggtctgaa ccaggtccgc
     1201 ctcgacggca aggggcgcct ggccattccg attcgctatc gcgcggagct gtccgacgtc
     1261 gcggccggcg cgctggtggc cacggtggac cgggatcgtt gtctcctgct gtatccgcgg
     1321 ccgcgctggg aggaaatcga agcgaaactg gtggagcttc cgagcctcca gccggaggcg
     1381 cgcgcggtgc aacgcatgct cctcggcaat gcgacggaac tcgagatgga cgggcatggc
     1441 cgcgtgctgc tcccgaccca tcttcgcagc tacgccgaac tcgatcacaa ggtggtgatg
     1501 atcggacagg ggcaccgttt cgaattgtgg agcgagacgc gctgggagga gagtgcgcgc
     1561 caatgggcgg ccggaatcgg catgagcgag cagggctcgg acgccctcgc gcggctgtcg
     1621 ctctgaccgg gcgatgcggg cgggatgcat gttccggttc ttctcgccga gacgctcgaa
     1681 tggctggccg tgcgccccgg cggaatttat gtggacgcca cctacggtcg cggcggccac
     1741 agcgcggcga ttctcgcgcg gctcgattcg accggacgcc tgtattcctt cgatcgcgat
     1801 ccgcaggcgt gcgccgatgc cgcccgtttt gcggccgatg gtcgcttcga gttcgtgcgc
     1861 gcgcccttct cgcggctcgg cggcattctc gaatcccgcg gtctcgccgg ccgcgtcgat
     1921 ggattgctct tcgatctggg cctgtcttcg ccgcaactcg acgatcccca tcgcggcttc
     1981 ggcattcgcc acgatggccc gctcgacatg cgcatggatc ccgaagccgg tccgagcgcg
     2041 ggcaagtggc tcgcgcgggc gagcgaacgc gagatcgccg atgtgatctt ccgctttggc
     2101 gaggagcgct acagccggcg gatcgcccgc gcgatcgtcg cgcgccggcg cgccgcgccc
     2161 atcgaaacga cgggcgaact cgccgccctc gtcgcccgag ccgtgccgcg gcgcgagcgg
     2221 cgtctgcatc cggcgacccg cagctttcag gcgctgcgca ttcacgtcaa cgacgaactc
     2281 ggggagttgc gcgcggggct tgcagcggcg gcgatgctgc ttgcaccgca agcccgtttg
     2341 ttggtaatca gttttcagtc cctcgaagat cgcatcgtca agcagtttct gcgcgaaggc
     2401 gcgcgcagcg atccgccgca atggaaaatc ctcgcgcggc tgctgcgtcc ggatcgcgaa
     2461 gaactcgcgc gcaacccccg cgctcgaagc gcgcgcctgc gggtcgcgga gcgctcggcg
     2521 tgaggctgcg tgggggaccc tcgctcgctc tgatcgccct cgcggcgatc gtggttttcg
     2581 gcaacgcgct ggcggttgtg tattcggctg cgcgcaatcg cgcgcttttc tccgaactca
     2641 actccctgcg cgatcaccgg gaagaattga tggcgaaccg cgggcggctc gaactcgaag
     2701 agtggaccct cgccgcccac agccgggtcg ccgacgtcgc gaccaagaag cttcacatgc
     2761 agccgccggc gacggtgcac atcgtggagg ttccatgaac cccgtggccg cgcgtcggac
     2821 tctcgcggtt gccgtgctgt ttgcgctcgc ggcgctgctg ctgatcgccc gggcggtgga
     2881 tctgcaggta atccgccacc cgctgctcgc cgatcgtgcc tcgcggcagc agacgcgcag
     2941 catggaactt cccgcgcacc gtggaatgat tctcgatcgg cgcggacagc cgctcgcgat
     3001 ttcgaccccg gtcgcggcga tctgggtcaa tcccaaggtg gtgagcggca gcggcgaatc
     3061 gctcgcgccg ctcgcgaaac tgctcgggct cgacgcagcg cgcctcgagg cggacgtgaa
     3121 ggcgcatgcg acgcgcagct ttctctatgt cgagcggggc ctgccgccga cgacggcgga
     3181 gcaagcgctc gcactcggcc ttgcgggcgt gcacagtcgc cgcgagtacc ggcgttatta
     3241 cccttcgggt cccgttgccg cgccggtgat cggcttcacc aatatcgacg atagcggcca
     3301 gtacggcctc gaactcgaat acgacgaatg gttgcaaggc aagcccgggg cgatgcgggt
     3361 cgtgaccagt gccaacggcc gccccgtgga ggacgacgag gtcatcgccg agccgcgcgc
     3421 cggtcgcgac ctcgtcacca gtctcgacat gcgcgtgcaa tacctggcat acgaggcgct
     3481 cgcggaagcc gtggcccgcc agcaggcggt gtcgggctcg gtggtggtgc tcgacgtcaa
     3541 gacgggcggc gtgctcgcga tggcgaatca gccctcctac aatccgaacg tccgctcgga
     3601 cctcaaggcg gcgctgtatc gcaaccgcgc cgtgacggat ctgttcgagc ccggatcctc
     3661 cttcaagccc ttcatcatcg cggcggcgct cgcttcgggc ggttggcgtc cgctgagcac
     3721 cgtggatacc ggaaatggca cgtggacggt gggcggctac acgatccacg acgacgagtc
     3781 gctcggcatc atcacgctca ccacgctgct cgaaaaatcg agcaacgtcg gcgccgccaa
     3841 ggttgcgctc agtctgcccg cagcctacct ctatcacgta cttaccggat tcggtttcgg
     3901 tcgcccggta gccggcggct tcccgggatc ggtttccggc gaactttctc cctacagtga
     3961 ctggcgcagg gtggagcagg ccgcgatttc ccgtggctac ggcgtgtcgg tgacggcgct
     4021 cgaactggcg cagggctatc tcgcgatcgc cgacggtggt gtcttccgtc cggtgacctt
     4081 tctgcaccgc gatgaaagtc ccacgggaac gcgcgtcatt cccgccgcga tcgcagccga
     4141 gctgcgcgcg atgctcacga ccgtcgtctc cgacgaggga acgggccgcc gggcgcagat
     4201 cgccaattat cgcgtggccg gcaagacggg cacggcgcac atcta
//
LOCUS       DASUJG010000089         4551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1369901, whole genome shotgun sequence.
ACCESSION   DASUJG010000089 DASUJG010000000
VERSION     DASUJG010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369901"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VFX38_01735"
     CDS             complement(<1..315)
                     /locus_tag="VFX38_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020412393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5313617.1"
                     /translation="MTNRIAAGASAERIAPGTIMALVAMALAVFVLANDFTAMSVALT
                     QIEHDFNSDVATVQWVINAYALVFGVLIVTGGKLADMFGRRRIFFIGTAIFALFSFLG
                     GIA"
     gene            complement(405..2003)
                     /locus_tag="VFX38_01740"
     CDS             complement(405..2003)
                     /locus_tag="VFX38_01740"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5313618.1"
                     /translation="MIHPVWHRVHERQVLGEADRPIAVVGLLGMAFASFMMGSDVTTL
                     GIILPKVQESFATSVSTAQWVMNAYALVFGILIVAGGRLADIFGRRRMLIAGTAIFAA
                     AAMVAGAAPGVFWLIGARALMGAGGAMMWPAALAISYTLVPKSKAALAGGVILGALGL
                     GGSSGPLIAGALSEFASWRWVLFVNVPAAAVVLAIACFGLRLARTSGSGERIDYAGIS
                     VLAALLLALLLAFDQAADWGLRDPRILGLLGAAALLLLAFFFIERRAGDAALVPGALL
                     RRRDLAASLASRFFIGGNYFVALVYLPEFMEKVLGYPPLAAGVGMIPMMLGFALTSFA
                     AGPLYPRWGAKYLCAVGMGVVVVGIALLMRLDAHSGYGVVLPGLLLVGVGYGLGSIAL
                     NTASVLAVPPAHASLAGAMLYMAQLVGGSLGLGAYTLIFSASAIHSLNAAGAAAGFAS
                     DQLAAASRLLLGAGSGNSLLARFGASADALHALARDSFVVGLQTGFLLLLALGALSAL
                     TALLFLGNWRLPRAAPGVESLARD"
     gene            complement(2000..2962)
                     /locus_tag="VFX38_01745"
     CDS             complement(2000..2962)
                     /locus_tag="VFX38_01745"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HEX5313619.1"
                     /translation="MSTKPQSEPGGGAGESFAASVLMVRPRSFAANPQTAPTNRFQTL
                     AATDDSERLRAQEEFDGLVNALVEAGIEVIVEEDTPEPEKPDAVFPNNWFSTHADGSA
                     VLYPMLVPSRRAERRPELLTGLRNRGFSLTAIVDLAGWERHGLALEGTGSLALERRAR
                     LAYVCRSPRSAEAPLVQWARLMGYRVLSFAATDGAGVAIYHTNVMLALGKGFALLGAE
                     CIADADERREVIASLEAGGREVIALSQEQIAGFGANALQLEGSRGAVIAMSNAARASL
                     GPEATKRLEHHGAIVAAPIPTIERYGGGSVRCMLAEIFLPRTPV"
     gene            3000..4076
                     /locus_tag="VFX38_01750"
     CDS             3000..4076
                     /locus_tag="VFX38_01750"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX5313620.1"
                     /translation="MSRQASENATGAGGQSPVRSGALLPRIAVRDENGEPGDLHAALE
                     GQPFVLLALARPRSPACRQAVRRFAALEADVPRLARVLLLPSETRGEDAAELSLPGLR
                     RYVATASGGLAPAAEDLAYVIGDAAGRVLASGEIRPNQLPQLGARLRNAFAPAKSQEN
                     APLDRAAPVLMVPDFLSPPLCRRLIAYFDRDGGHPSGVLDLSGPTPAWHPDEAIKRRR
                     DLLLEDRELIRELEETAAEKLLPQIRKCFHYVVTRHEPFKLACYDEGSGYFRPHRDNE
                     TSDTLHRRFAMTANLNTGDYEGGALNFPEFGSAIYRPPRGGAIVFSSSLLHEATDVTR
                     GRRYVLIGFFYNPADGLTGSEKAG"
     gene            complement(4081..>4551)
                     /locus_tag="VFX38_01755"
     CDS             complement(4081..>4551)
                     /locus_tag="VFX38_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5313621.1"
                     /translation="LAELASGYRHRQGRLVRVLRPWPWTPWGPAGALRSNARDLLHYT
                     AAYLGAEQAGDVAVPLELSRAMALARAPADVKLVDGSGSQAYAWVVRNADNPARSMVY
                     KNGGTAGYSSCIALWPAGKTGVVLLMNSSKTHACKKGLALLRILAGPPQKRPGR"
BASE COUNT          818 a         1553 c         1514 g          666 t
ORIGIN      
        1 cgcgatgccg ccgagaaagg aaaaaagcgc gaagattgcc gttccgatga agaagatgcg
       61 ccgccggccg aacatgtcgg cgagcttgcc gccggtcacg atcagcacgc cgaatacgag
      121 cgcgtaggcg ttgatcaccc actgcaccgt tgccacgtcg gagttgaaat catgctcgat
      181 ctgggtgagc gcgacgctca tcgcggtgaa atcgttcgcg agcacgaaga ccgcgagcgc
      241 catcgcgacc agcgccatga tcgttcccgg agcgattctt tccgccgacg caccggcggc
      301 gatgcggttt gtcacgtttc ccatccccga gcgtccgtgc cttcgcatca taccggatga
      361 aaatttcggg agcaggcgcc gccgtcggag cgcgccgcgc tgggtcagtc gcgagcgaga
      421 ctctccactc ccggcgcggc gcgcggcagg cgccagtttc cgagaaacag cagcgccgtc
      481 agtgcggaca gcgcgcccag cgcaaggaga agcaggaatc cggtctggag tccgacgacg
      541 aaggaatcgc gcgcgagcgc atggagcgca tccgccgaag cgccgaaacg ggcaagaagc
      601 gaatttcccg agcccgctcc gagaagaagc cgggaagcgg cggcgagttg atcggacgcg
      661 aatccggccg cggctccggc cgcgttcagg ctgtgaatcg cgctcgcgct gaagatcagc
      721 gtgtaggcgc cgagcccgag ggaaccgccg acgagttgcg ccatgtagag catcgccccg
      781 gcgagactcg catgcgccgg cggcaccgcg agcacgctcg cggtgttgag cgcgatcgaa
      841 ccgagaccgt agccgacgcc gacgagaagg aggcccggaa gaacgactcc gtaacccgaa
      901 tgtgcatcga gccgcatgag aagcgcgatg ccgacaacga cgacccccat gccgactgcg
      961 cagagatact tcgcgcccca gcgcggatag agcggacccg ccgcgaagga ggtgagggcg
     1021 aatccgagca tcatcggaat catcccgact ccggccgcga gcggcggata gccgagcacc
     1081 ttctccatga actcgggcag atacacgagt gcgacgaaat agtttccgcc gatgaaaaag
     1141 cgcgaggcga gcgaggccgc gagatcacgc cgccgcagaa gcgctccggg aaccagcgcc
     1201 gcatcgccgg cgcgccgttc gatgaagaaa aacgcgagga gcagcaacgc ggcggcaccg
     1261 aggaggccga gaatgcgcgg atcgcgaagt ccccaatcgg ccgcctgatc gaaggcgaga
     1321 aggagtgcga gcagcagcgc cgcgagaacc gaaatcccgg cgtagtcgat gcgctcgccg
     1381 cttccggacg tgcgcgcgag gcgcaatccg aagcaggcga tcgcgagcac gacggccgcc
     1441 gccggcacgt tgacgaaaag cacccagcgc cagctcgcga actcggagag ggcgccggcg
     1501 atcagcggac cggagctgcc gccgagcccg agcgcaccga ggatcacgcc gccggcgagc
     1561 gccgccttcg atttgggcac gagcgtatag gaaatggcga gcgccgcggg ccacatcatc
     1621 gctccgcccg cacccatcaa ggcacgcgcg ccgatcagcc agaagactcc gggcgcggct
     1681 cccgcgacca ttgccgccgc tgcgaagatc gcggtgccgg cgatgagcat gcgccgccgc
     1741 ccgaagatgt cggcgagccg cccgcccgcg acgatcagaa tgccgaacac gagcgcgtag
     1801 gcgttcatca cccactgcgc ggtgctgacc gaggtcgcga aactttcctg caccttcggc
     1861 aggatgatgc cgagggtggt gacgtcgctt cccatcatga aggaggcgaa ggccatgccg
     1921 aggagtccga cgacggcgat cggccggtcc gcttcgccca agacctggcg ctcgtgtacc
     1981 cgatgccaga ccggatgaat cacaccggcg tgcgcggcag gaaaatctcg gcgagcatgc
     2041 agcgcacgct gccgccgccg tagcgttcga tggtcggaat cggcgcggcg acgatcgcgc
     2101 cgtgatgctc gagtcgtttc gtcgcctccg gcccgagact ggcacgggcc gcattggaca
     2161 tcgcgatcac cgcaccgcgg ctcccttcca gttgcagcgc gttggcgccg aagcccgcaa
     2221 tctgctcctg cgaaagtgca atcacttcgc gtccgccggc ttcgagactt gcgatgacct
     2281 cgcgccgctc gtcggcgtcg gcaatgcatt ccgcgccaag gagcgcgaat cccttcccga
     2341 gcgcgagcat cacgttggtg tgatagatcg ccacaccggc gccatccgtt gccgcaaacg
     2401 agagcacgcg atatcccatc agtcgcgccc attgcacgag cggcgcctcg gcactccgag
     2461 gcgagcggca gacgtacgcg aggcgtgcgc gccgctcgag cgcgagcgag ccggtgcctt
     2521 cgagcgcgag accgtggcgc tcccaaccgg cgagatccac gatggcggtg agcgagaagc
     2581 cgcggttgcg caggcccgtc agcagttcgg gtcggcgctc ggcgcgtcgg ctgggcacga
     2641 gcatcggata gagcaccgcg ctgccgtcgg catgcgtcga gaaccagttg ttcggaaaca
     2701 cggcgtcggg tttctcgggc tcgggcgtgt cctcctccac gatcacctcg atgccggcct
     2761 cgaccagcgc attcacgagc ccgtcgaatt cttcctgtgc ccgcagccgc tcggagtcgt
     2821 cggttgcggc gagcgtctgg aaacggttgg tcggcgcggt ttgcgggttc gcagcgaagc
     2881 tccgcggtcg caccatgagc acgctcgcgg cgaaactctc gccggcgccg ccgccgggct
     2941 cggactgggg ctttgtgctc atcggcaaca tccgggcttt gatgctattg tctcccatca
     3001 tgagcagaca ggccagcgaa aacgccacgg gcgccggcgg ccagagcccg gtgcggtcgg
     3061 gggcactgct gccgcgcatc gcggtgcgcg acgaaaacgg cgaaccgggg gatcttcatg
     3121 cggcacttga ggggcagcct ttcgtgctgc tcgcgctcgc ccgcccgcgc agtcccgctt
     3181 gccgccaggc cgtgcgccgc ttcgccgcgc tcgaagccga cgtgccgcga ctcgcgcgcg
     3241 tattgctgct gccgtcggag acccgcggag aagacgccgc cgaactctcc ctgcccggac
     3301 ttcgccgcta cgtcgcgacc gcgtccggcg gactggctcc ggcggcggaa gacctcgcct
     3361 acgtcatcgg tgatgcggcc ggccgcgtgc tcgcgagtgg cgaaatccgg ccgaatcaac
     3421 tcccgcagct cggcgcgcgc ctgcgcaacg cattcgcgcc ggcaaagtcg caggaaaacg
     3481 ctccgctcga tcgcgccgcg ccggtgctca tggttcccga tttcctttcg ccgccgctct
     3541 gccgtcgcct gatcgcgtac ttcgatcgcg acggcggaca tccctcgggc gtgctcgatc
     3601 tttccggtcc gacgcccgcg tggcatcccg acgaggcgat caagcgccgg cgcgatctgc
     3661 tgctcgagga tcgcgagttg atccgcgaac tcgaagagac ggcggcagaa aagctgctgc
     3721 cgcagatccg caaatgcttt cattatgtcg tgacgcgcca cgagcccttc aagctcgcgt
     3781 gctacgacga aggcagcggc tatttccgtc cgcatcgcga caacgaaacg agcgacaccc
     3841 tgcaccgccg tttcgcgatg acggcgaatc tcaataccgg cgattacgaa ggcggcgcgc
     3901 tcaactttcc ggaattcggc agcgccatct accgtccgcc gcggggcggc gcgatcgtct
     3961 tttcgagttc gcttctgcac gaggcgaccg acgtcacccg cgggcgccgc tacgtcctca
     4021 tcggcttttt ctacaatccc gccgacggcc tcacggggag cgaaaaggcc gggtgagccg
     4081 ctatcttccc ggacgtttct gcggcggccc cgcgagaatc cgcagcagcg cgagaccttt
     4141 tttgcaggca tgcgtcttgc tgctgttcat gagcagcacg acgcccgttt ttccggcggg
     4201 ccagagcgca atgcagctcg aatagcctgc cgtgcctccg tttttgtata ccatcgagcg
     4261 cgccgggttg tcggcgttcc gcacgaccca ggcgtaggcc tggctcccgc tgccgtcgac
     4321 aagtttcaca tcggcgggtg cgcgcgcgag cgccattgcc ctggaaagtt ccagcggcac
     4381 cgcgacgtcg cccgcctgct cggctccgag ataggcggcc gtgtagtgga gaagatcgcg
     4441 ggcattggaa cgaagcgcac ccgccggacc ccagggcgtc cagggccagg gacgaagcac
     4501 gcgaacaagg cggccttgtc gatggcgata cccgcttgcg agttcggcca g
//
LOCUS       DASUJG010000090         2630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_391835, whole genome shotgun sequence.
ACCESSION   DASUJG010000090 DASUJG010000000
VERSION     DASUJG010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391835"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VFX38_01760"
     CDS             complement(<1..706)
                     /locus_tag="VFX38_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5313622.1"
                     /translation="MSEAYEAIVIGGGHNGLVCACYLARGGLKTLVLERRGLVGGAAV
                     TEEFHPGFRNSTASYTVSLLHSKVIRELHLAEHGLKIVERPLANFLPLPDGNYLKVGP
                     DTAATQREFARFSKHDAERLPDYEAMLGCAANALRELAVVTPPAAGAGLPAMFRAARE
                     GRKLWKLPLAVQRDLLALFTRSAAEFLDVWFESQPVKAVFGFDSVVGNYASPYTPGSA
                     YVLLHHVFGEVNGRRGA"
     gene            987..1877
                     /locus_tag="VFX38_01765"
     CDS             987..1877
                     /locus_tag="VFX38_01765"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5313623.1"
                     /translation="MKKFVAIVLGAIACAVMISAGSGVRAASTQTADGPPPGDRVVCV
                     SKQINEFIYDIGAENHLVAHDLTSVYPPQITKLPSVGYHRALSAEGIISMHPSVFLTD
                     GNVGPQAVLDQLRKVGIPVKVMDPGETMEQSQQLMLRLGRYFDREKAAETIVAKWRRG
                     MAQVKQESRRWAGKPRPRVLMIHFGQIINNYLAVTSGTPAGQIIEWAGGVNAVSAKGG
                     MVRLTPELIAESKPDVIIANNVAFDRFGSAKKFEQLPGVALTPAGKSGRIYMIPEQAI
                     IYFGPRTPATVKELAKMLHP"
     gene            2337..>2630
                     /locus_tag="VFX38_01770"
     CDS             2337..>2630
                     /locus_tag="VFX38_01770"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEX5313624.1"
                     /translation="MLRRLSYGQVYILLGALLIAAMIASAGIGATTFTPPDMLRYIGQ
                     AFGWIHASDQDALQRNVFLLLRLPRVLMAGLAGAVLGVAGTLMQGLFRNPIVEP"
BASE COUNT          463 a          894 c          815 g          458 t
ORIGIN      
        1 aggcgccgcg cctgccgttc acctcgccga aaacatgatg cagcagcacg taggccgagc
       61 cgggcgtgta cgggctcgcg tagtttccga ccaccgaatc gaagccgaac accgccttca
      121 ccggctgcga ctcgaaccag acgtcgagaa actcggccgc actgcgggtg aaaagtgcga
      181 gcagatcgcg ctgcacggca agcggcagct tccacagttt gcgtccttcg cgcgcggcac
      241 ggaacatcgc cggcaggccc gcgcctgcgg cgggcggcgt caccactgcg agttcccgaa
      301 gcgcattcgc tgcgcatccc agcatcgcct cgtagtccgg gaggcgctcg gcgtcatgct
      361 tgctgaagcg cgcgaattcg cgctgcgtcg ccgccgtatc cggtccgacc ttgaggtaat
      421 tcccgtcggg cagcggcagg aaattcgcga gcggccgctc gacgatcttc agtccatgtt
      481 ccgcaagatg aagctcgcga atcaccttcg agtgcagcag gcttaccgtg tagctcgcgg
      541 tcgagttgcg gaagcccgga tgaaactcct cggtcacggc cgcgccgccg accaggccgc
      601 gccgctcgag taccagggtt ttcaatccgc cgcgcgcgag atagcaggca cagacgagtc
      661 cgttgtgccc gccgccgatg acgattgctt cgtacgcttc gctcatttca tcgcactcgt
      721 cttctcccgt tcgggccgca catcgaagct tcgccaccgc cgaaatcacc ggggacgcgc
      781 aactccggat tatatcgccg gcccggcgcc cgggttgcgt tgcgcaagca gttcggccta
      841 gtgaatggtc gtacaggcga gtggctcttc cgccggaatc gagcgccttc gatccgcccg
      901 cgacgatcgg gcgtgccgcg ctgccgggcc tgtgctacga ttcggaaccc cgtcgggccg
      961 cactcgcgga atcagaggaa cagggaatga agaagttcgt tgcaatcgtc ctcggcgcca
     1021 tcgcctgcgc ggtgatgatt tccgccggct ccggcgtccg cgcggcatcc acgcagaccg
     1081 cggacggtcc gccgcccggc gatcgcgtgg tctgcgtctc caaacagatc aacgaattca
     1141 tctacgacat cggcgccgaa aaccatctcg tggcccacga tctcacgtcc gtttatccgc
     1201 cgcagatcac caaattgccg tcggtcggct accaccgggc gctgagcgcc gaggggatca
     1261 tctcgatgca ccccagcgtg tttctcaccg acggcaacgt cggcccgcag gcggtgctcg
     1321 atcagttgcg caaggtcggc attcccgtga aggtgatgga cccgggcgaa accatggagc
     1381 agtcgcagca gctcatgctg cgcctcggcc gatatttcga ccgcgagaag gcggccgaga
     1441 cgatcgtcgc gaagtggcgc cgcggcatgg cgcaggtcaa gcaggaaagc cgccggtggg
     1501 ccggcaaacc caggccgcgc gtgctgatga ttcatttcgg ccagatcatc aacaactatc
     1561 tcgccgttac cagcggcacg ccggcgggac agatcatcga atgggccggc ggagtcaacg
     1621 ccgtcagcgc gaagggcggc atggtacgcc tcacgcccga gttgatcgcc gagtccaaac
     1681 ccgatgtcat catcgccaac aacgtcgcct tcgaccgttt cggcagcgcc aagaagttcg
     1741 agcaactgcc gggtgtggcg ttgacgccgg cgggaaagtc cgggcgtatc tacatgatcc
     1801 ccgagcaggc gatcatctac ttcggaccgc gcacgccggc gacggtgaaa gaactcgcga
     1861 agatgctcca tccctgacag cttgccgaaa actgctgcgc gcggcatttt gtaccttact
     1921 tccctcttcg ctcgcgaagc tcgctgcttt tccccctttc gtgtgcgaag ggggacgaag
     1981 ggggatgtct tccccgcgca gcacggaaca tcccccgcgc aagagcgcgg ccccgcctcg
     2041 ctccgaagct cgctgcattt cccccttcgc ttgcgaaggg ggacgaaggg ggatgtcttc
     2101 ccggcgcagc atggaacatc cccccgcgca agcgcggccc cgcctcgctc cgaagctcgc
     2161 tgcttttccc ccttcgcgtg cgaaggggga cgaaggggga tgtcttcctt cgtcccgaag
     2221 agggcatcga agcaatcgga acgaaagtgt ggcattgcct gtaacgccat gtccagctac
     2281 cgttcgcaag tcatgaacat ccacgggact cggtctcgtt cgcggacgga ctccagatgc
     2341 tgaggcggct ctcgtatggg caggtctaca tcctgctcgg cgctctgctg atcgccgcga
     2401 tgatcgcatc ggccggcatc ggcgcgacca cgttcacgcc gcccgacatg cttcgctaca
     2461 tcggccaggc cttcggctgg attcacgcga gcgaccagga cgccctgcaa cgcaacgtgt
     2521 ttcttctgct gcgtctgccg cgcgtcctga tggccgggct cgccggcgcg gtgctcgggg
     2581 tcgccggaac cctgatgcag ggactcttcc gcaacccgat cgtcgagccg
//
LOCUS       DASUJG010000091         5814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_635362, whole genome shotgun sequence.
ACCESSION   DASUJG010000091 DASUJG010000000
VERSION     DASUJG010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5814
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635362"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1141)
                     /locus_tag="VFX38_01775"
     CDS             complement(173..1141)
                     /locus_tag="VFX38_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5313625.1"
                     /translation="MSEPAIARYRDGIHAIDTGYVRPGLDASHLVLRDGRAAFVDTGT
                     NHSVPLLLAALAALGIARETVDYVILTHVHLDHAGGAGALMRELPHATAIVHPRGLPH
                     LVEPQKLEAGTRAVYGDHVYETLYGKLVPIAAERIRSVADEETLTLGRSRLRFLHTPG
                     HALHHVAIHDETANAVFTGDIFGISYRVFDNPEGSPFVFPTTTPTQFDPEQMHASIER
                     IRALRPRALYLTHYAQVTEIERMAEDLHRDVDRFVDIAKLHADEPEPEVPMRHAMHDY
                     LAGRLLAHGSGVSPKMRETWLAMDVNLNAAGLLAWHRRLDRTRAPQ"
     gene            1608..3068
                     /locus_tag="VFX38_01780"
     CDS             1608..3068
                     /locus_tag="VFX38_01780"
                     /inference="COORDINATES: protein motif:HMM:NF017870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HEX5313626.1"
                     /translation="MIRRIFASVGILIALLIIAVVVLPYFVGLRAEQGFEYQVRRFNA
                     SHAIFRLRVDAYHRGFYSSDADVTLRGTTKQSSRFFGALFGTGAKAQFHIRINHGPIP
                     FAAFGAEHVNFLPVLYTAEFRGRNLPPLSFLGALKPDVYLVQYFGGATRAHIAVPSGR
                     FGLGAAGARWDGGEATIRVDSAHDRSEYRGTIAPIEFQYMDTSLGVTYDTQVQPIEFS
                     GTRSKAKHDLWVGTGSASFAGLSVSSEDGELVRLAGAKTSSAIRESADGRWIGGHVRL
                     DQDGGVIRGWRFSRFSVEGSVLRLDAAALDRGLRALRQQSTRVAKQLTASRARVVAGN
                     ALAPAITTATRAEVAATLAAASGSAKFAAHLGFTRASATSVGIPGASLRRLAVGEGSL
                     DFDRKLANDFATKVLGPAFAMLIEQSLQGLVRQGYLTVNAAGACHALLGYRAGVLTVN
                     GRSVSGVTQAPAARTAQRAGGGRLRAMRGAEGSPIP"
     gene            3102..3497
                     /locus_tag="VFX38_01785"
     CDS             3102..3497
                     /locus_tag="VFX38_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX5313627.1"
                     /translation="MRSSEFEGRMWAEAVALFERAERLKRQFFLPPGSASGSACWAPP
                     LDVFETAMEFVALVALPDVDPAHIELSFADGVLVVAGERRLPIALAGAAIRRLEIPQG
                     RFERRLALPARSLARSEFRNGCLLVVLEK"
     gene            3494..>5814
                     /gene="lon"
                     /locus_tag="VFX38_01790"
     CDS             3494..>5814
                     /gene="lon"
                     /locus_tag="VFX38_01790"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX5313628.1"
                     /translation="MNDETAQADPEVLTTASVVPLVPLREAVLFPGMVLPFAVGRRGS
                     VAAVEAAIKGERPVGLILQRDRAQEEPGPEGVHSVGTLANVLRFLTAPDGTNHLICQG
                     ISRFRASDIKREGDYLTVHAEPLPDPGGEPDAELQAQSLHLRQQALEALQLLPQTPDE
                     LVNTVRAIREPGSLADLVAGYLDLAAEEKQRILETTDLPARLREVSRQLAQRLEVLRI
                     SSRIREETQQAMGEKQKEFILREQLRQIQKELGEGDDAKGAEIQELDGKIAEAKMPEA
                     VEKQARSELKRLERMPEAAAEYSMVRSYLDWLIEFPWAKETDDNYDLAAARKVLDADH
                     YDLDKIKRRIVEFLAVRKRNPKGHAPLLCFVGPPGVGKTSLGQSIARAMGRKFERMSL
                     GGVHDEAEIRGHRRTYIGALPGNIVRAMKKAGTRNPVIMLDELDKLGTGFQGDPAAAL
                     LEVLDPAQNDTFRDSYLDVEVDLSAVIFIGTANVLDQIPGPLRDRLEMIELPGYTRSD
                     KLEIAHRYLIARQRAENGLEESEIEIPDVTLAAIIEQYTREAGVRQLEREIGSVCRAI
                     TTRIAEGKAGAYTIRPEDLHEILGPRKFEDEAALRTSVPGVATGLAWTPVGGQILFIE
                     ATATPGKGNLHLTGQLGNVMKESVEIALSLIKSRAEKLGIDASVFTDRDIHVHVPAGA
                     IPKDGPSAGVTMTTALVSLFTGRCVRADLAMTGEITLRGLVLPVGGIKEKVLAAHTAG
                     IKRLILPARNRKDFEDIPEPAREALEFVWAEEL"
BASE COUNT          989 a         1894 c         1985 g          946 t
ORIGIN      
        1 atgatgcggc ccttgccggt gagcgcgagc gcgtctgtgt agttgaggcc ggaatattcg
       61 ccgcggatgg tgaccgcgcc cgcgccgaga tcgtcgagcg cgagcgattc gaggcgcgct
      121 tcggtgcccc cgcccgcacg atgcacgcgc caggcgggaa actgcgggtc gctcattgcg
      181 gcgcgcgcgt tcggtcgagg cggcgatgcc aggcgagaag tcccgcggcg ttgagattga
      241 cgtccatggc gagccaggtt tcgcgcatct tcggtgacac gccgctgccg tgcgcaagga
      301 gccgcccggc aagatagtcg tgcatcgcgt ggcgcatggg aacctcgggt tcgggctcgt
      361 ccgcgtgaag cttcgcgata tccacgaagc gatccacgtc gcgatgcaga tcttcggcca
      421 tgcgttcgat ttccgtcacc tgcgcgtagt gggtcaggta caacgcgcgc ggacgaagcg
      481 cgcggatgcg ctcgatcgag gcgtgcatct gctccgggtc gaactgcgtc ggcgtggtcg
      541 tcggaaatac gaagggcgag ccttcgggat tgtcgaagac gcggtaggaa atgccgaaga
      601 tgtcgccggt gaacaccgcg ttcgcggttt cgtcgtgaat cgcgacgtga tgaagggcgt
      661 ggcccggagt gtggagaaag cgcaggcgcg aacgtccgag cgtgagcgtc tcctcgtcgg
      721 cgacgctgcg aatgcgctcg gcggcaatcg gaacgagttt tccgtagagc gtctcgtaca
      781 cgtgatcgcc gtacacggct cgcgttcccg cctcgagttt ctgcggctcg acgagatgcg
      841 gcagcccgcg cggatggacg atcgcggttg cgtgcggaag ttcgcgcatc agcgcgccgg
      901 cgccgccggc gtgatcgagg tgcacatgcg tcaggatgac gtagtccacc gtctcgcgcg
      961 caatgcccag cgccgccagt gcggcgagca gcagcggaac cgaatgattg gtgccggtat
     1021 cgacgaaggc cgcccgcccg tcgcgcagca ctaggtggct tgcgtcgagc cccgggcgca
     1081 catagcccgt gtcgatcgca tgaatgccgt cgcgataacg ggcaatggcg ggttcgctca
     1141 agcgatcctc cgaacgcggg atgtttgatt ctacaccggc gcattgaccg cgcccggccg
     1201 ccgcgatatc gtcgcccgat ccgcaagccg gcggaaattt tgtcggagat cgcgctggcc
     1261 gcagcgatgc tctcgcgcgg tttatggccg gttgaacgtt gcaaatgata ccggcgtcaa
     1321 gtggcttttg cggcgcgacg ccacgctcgt cgtcagcggc cacccgcggg cgtcgccggc
     1381 ctagccgccg gtcacacctg cgggcgcgag cgctggcggg ccgcgaatcg agccgcagcg
     1441 ggggaatccc cgggatttcg gcgcgatcgc cgggccgatc ggaccccggc gcaagcgaca
     1501 acataaaata gcggtgcgac gccgggcgga ggcgacatga aacggatgga tccggaaaca
     1561 cccggggcga tgcgtgcctt gaaggggctg cgacgcggag cgccgcgatg atccggcgta
     1621 tcttcgcgag cgtcggaatc ctgatcgcgc tgctgatcat cgcggtcgtc gtgcttccgt
     1681 atttcgtcgg actgcgcgcg gagcagggct tcgagtatca agtacgccga ttcaacgcct
     1741 ctcacgcgat cttccgtctg cgggtggatg cctaccatcg cggtttctat tcctccgacg
     1801 ccgacgtgac cctgcgcgga acgacgaagc agagcagccg cttcttcggc gcgctcttcg
     1861 gcacgggcgc gaaggcgcag tttcacattc gtatcaacca cggcccgatt cccttcgcgg
     1921 ccttcggcgc ggagcatgtg aacttcctgc cggtgctcta cacggccgag ttccgcggca
     1981 gaaacctgcc gccgctgtcg tttctcggcg cgctcaagcc cgatgtctat ctcgtgcagt
     2041 atttcggcgg cgcgacccgc gcgcacatcg ccgtgccttc gggacgcttc ggactcggtg
     2101 cggcgggcgc acgctgggac ggcggcgagg ccacgattcg cgtcgactcc gcccatgatc
     2161 gctcggagta ccgcggaacg atcgcgccga tcgagttcca gtacatggac acgagtctgg
     2221 gcgtgaccta tgacacgcag gtgcagccga tcgagttttc cggcacgcgc agcaaggcga
     2281 agcacgacct gtgggtcgga accggcagcg cgagtttcgc gggcctgagc gtcagttccg
     2341 aggacggcga actcgtccgg ctcgccggcg caaagacgag cagcgcaatt cgcgaaagcg
     2401 ccgacgggcg ctggatcggc ggccatgttc gactcgatca ggatggcgga gtcatccggg
     2461 gctggcgttt ttcgcgcttt tccgtcgagg gctcggtgct ccgcctcgat gccgcggcgc
     2521 tcgatcgtgg gcttcgggcg ctgcgccaac aatcgacgcg cgtcgcgaag caactcactg
     2581 cctcgcgcgc ccgtgtcgtg gcggggaatg ccttggctcc cgcgatcacg accgcgaccc
     2641 gggccgaggt cgcggcgacg cttgccgccg cgagcggcag cgcgaaattc gcggcgcatc
     2701 tcggtttcac ccgagcctcc gcgacgagcg tcggaattcc gggcgcctcg ctccggcggc
     2761 tcgcggtcgg cgagggaagc ctcgacttcg atcgcaagct cgcgaacgac ttcgcaacga
     2821 aggtgctcgg cccggccttc gcgatgctga tcgagcaatc cttgcagggg ctggtgcgcc
     2881 agggttatct caccgtgaat gcggcgggcg cctgccacgc gctccttggc taccgcgccg
     2941 gcgtgctcac cgtcaacgga cggagcgtca gcggcgtcac gcaggctccg gcggcccgca
     3001 cggcgcaacg cgccggcggc ggaaggctcc gggcgatgcg cggggcggag ggctcgccga
     3061 tcccctagaa tgaaggcttc gattcccgag tgcgaggcag gatgcgaagc agcgaattcg
     3121 aaggacggat gtgggccgag gccgtggcgc tgttcgagcg tgccgagcgg ctcaaacgcc
     3181 agttctttct tccgcccgga tcggcgagcg gcagcgcctg ctgggccccg ccgctcgatg
     3241 tcttcgaaac cgcgatggag ttcgtcgcgc ttgtggccct tccggacgtc gatcccgcgc
     3301 acatcgaact cagctttgcc gacggtgtgc tcgtggtcgc cggcgagcgg cgtctaccga
     3361 tcgcgctcgc gggcgccgcg atccggcgac tggagattcc gcagggccgt ttcgagcgcc
     3421 gcctcgcgct tcccgcacgt tcgctcgcgc gctcggaatt tcgcaacggc tgcctcctcg
     3481 tggtgctcga gaaatgaacg acgaaaccgc gcaagccgat ccggaagttc tgacgacggc
     3541 gagcgtcgtg ccgctggtgc cgctgcgcga ggcggtgctt ttccccggca tggtgctgcc
     3601 cttcgccgtt ggacggcgcg gctcggttgc ggcggtggaa gcggcgatca agggcgagcg
     3661 gcccgtcggg ctcattctgc aacgcgatcg cgcgcaggag gagccggggc ccgaaggcgt
     3721 gcactcggtc ggaacgctcg cgaacgtgct gcgctttctc accgctcccg acggcaccaa
     3781 tcatctcatc tgtcaaggaa tcagccgctt tcgcgcgagc gacatcaaac gcgaaggcga
     3841 ctatctcacg gtgcatgccg agccgcttcc ggacccgggc ggcgaacccg acgcggaact
     3901 gcaggcgcag tcgctgcatc tgcgccagca ggcgctggag gccctgcagc ttctgccgca
     3961 gacgcccgac gagctggtga acaccgtacg cgccattcgc gagccgggaa gtctcgccga
     4021 tctcgtggcg ggctatctcg atctggcggc cgaggagaag cagcgcatcc tcgagacgac
     4081 cgatcttccg gcgcgcctgc gcgaagtgag ccggcagctc gcgcagcgtc tggaagtgct
     4141 tcgcatctcc tcgcgcatcc gcgaggagac gcagcaggcg atgggcgaaa agcagaagga
     4201 attcatcctg cgcgaacaac tgcgccagat tcagaaggaa ctcggcgagg gcgacgatgc
     4261 caagggtgcg gagattcagg aactcgacgg caagatcgcc gaggcgaaga tgcccgaagc
     4321 agtggaaaag caggcacgga gcgaactcaa gcgcttggag cgcatgcccg aggccgcggc
     4381 cgagtactcg atggttcgca gctacctcga ctggctgatc gagttcccgt gggcgaagga
     4441 aaccgacgac aattacgatc tcgccgcggc gcgcaaggtg ctggatgcgg atcactacga
     4501 tctcgacaag atcaagcggc ggatcgtgga gtttctcgcc gtgcgcaagc gcaacccgaa
     4561 ggggcacgca ccgcttctgt gtttcgtcgg accgcccggc gtcggcaaga cctcgctcgg
     4621 ccagtcgatc gcgcgggcga tggggcgcaa gttcgaacga atgagtctcg gcggcgtgca
     4681 cgacgaggcg gaaattcgcg gccaccggcg cacctacatc ggcgcgctgc cgggcaacat
     4741 cgtgcgcgcg atgaaaaagg ccggcacccg caaccccgtc atcatgctcg acgaactcga
     4801 caagctcggc acaggctttc agggcgatcc cgcggcggca cttctcgaag tgctcgatcc
     4861 ggcgcagaac gacacctttc gcgattccta tctcgacgtc gaagtggatc tttccgccgt
     4921 catcttcatc ggcacggcca acgtgctcga tcagattccg ggtccgcttc gcgatcggct
     4981 cgagatgatc gaactgcccg gctatacgcg cagcgacaaa ctcgagatcg cacatcggta
     5041 cctgatcgca cgccagcgcg ccgaaaacgg tctcgaggaa agcgaaatcg aaatccccga
     5101 cgtcacgctc gccgcgatca tcgaacagta cacccgcgag gcgggcgtgc gccagctcga
     5161 acgcgaaatc ggctccgtgt gccgcgcgat cacgacgcgc atcgccgagg gcaaggccgg
     5221 agcgtacacg atccgccccg aggatttgca cgagattctc ggcccgcgca agttcgagga
     5281 cgaggcggcg cttcgcacca gcgtgcccgg cgttgcgacc ggacttgcct ggacgccggt
     5341 cggcgggcag attctcttca tcgaggcgac ggcgaccccc gggaaaggca atctgcactt
     5401 gaccggccag ctcgggaacg tgatgaagga aagcgtcgag atcgcgctct cgctcatcaa
     5461 gtcgcgcgcc gagaaactcg gcatcgacgc ttccgtgttt accgatcgcg acatccacgt
     5521 gcacgtgccg gcgggcgcga ttcccaagga cggcccgagc gcgggcgtga cgatgaccac
     5581 ggcgctggtg tcgctcttca ccgggcgctg cgtgcgcgcc gatctcgcca tgacggggga
     5641 aatcacgctc cgcggtctcg tactgccggt cggcggcatc aaggagaagg tgctcgccgc
     5701 gcacaccgcc ggcatcaagc gcttgattct tcccgcgcgc aaccggaagg acttcgaaga
     5761 cattcccgaa cccgcccgcg aggcgctcga attcgtctgg gccgaagaac tcga
//
LOCUS       DASUJG010000092         4465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1370288, whole genome shotgun sequence.
ACCESSION   DASUJG010000092 DASUJG010000000
VERSION     DASUJG010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370288"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="VFX38_01795"
     CDS             <1..840
                     /locus_tag="VFX38_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HEX5313629.1"
                     /translation="IYTEALSSAPGSVAQLRESTALLVRYAKQAGVAVFLVGHVTKQG
                     AIAGPRVLEHMVDTVLYFENDAGSRYRAIRAVKNRFGAANELGVFAMTETGLKEVRNP
                     SAIFLAREGAPSAGSVVTVLREGTRPLLIEVQALVDESPLANPRRVAVGTDANRLGML
                     LAVLHRHAGRSFHGHDVFVNVVGGLKVTETGIDIALAAALVSSLADKPLPADLVVFGE
                     VGLGGEVRPVYGGEERLAEAAAQGFKRAIVPTANAPRSRRKPGLEVKSVRSLAEALAA
                     LFS"
     gene            complement(926..2008)
                     /locus_tag="VFX38_01800"
     CDS             complement(926..2008)
                     /locus_tag="VFX38_01800"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEX5313630.1"
                     /translation="MDRYQRQMLLPEIGVAGQKRLTAARVLVIGAGGLGSSALYYLAA
                     AGIGILAIVDDDRVERSNLHRQILYADADIGRNKAEAAAERLAAFNPDIRIEPKIVRF
                     DAGNAMELLARCDLVIDATDRLATKFLINDAAAKAARPLVHASALGFEARVALFDASR
                     GPCLRCLFPAPPEIPPATCAEAGVLGAVLGMAGSMQALEAIRWILAAGDDRSLREILG
                     RLWLFDGRSLATRIVALPRDPACEVCRHRPEDICLEDIERSVSDIAAEELERLADALF
                     VDVREREEWDAGHLSGALHLPLSRLAQADRIELPPSRAYVVYCAHGLRSQTAARILLA
                     AGYRNVRSLSGGLAALHGERSPPLSM"
     gene            2117..3490
                     /locus_tag="VFX38_01805"
     CDS             2117..3490
                     /locus_tag="VFX38_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HEX5313631.1"
                     /translation="MNEKTGPLGHPIRCFKAYDVRGRVPDELDENIAYRIARAWVDFI
                     GAKRVCMGRDVRPTSDAFADAVAAGLIDSGVEVRDLGICGTEESYFGTFHQGLDGGVM
                     ITASHNPSDYNGLKFVREGAHPISADNGLEDIRALAERGEFRDADAKGKRVPAVDKSA
                     YVAHLLSYVEVEKLKELRLVVNPGNGCAGPIVQQLERSLPFEFVKMDFEPDGSFPNGV
                     PNPMLEENRRETSAAVRAHKADLGIAWDGDFDRCFFYDEHGSFVEGYYIVGLLAEAFL
                     RRNPGARVLHDPRLTWNTVDIVREFGGTPIVSKSGHAFMKEKMRTEDCIYGGEMSAHH
                     YFRKFGYCDSGMIPWLLVAGLISATGTPLSALVADREAKFPASGEINREVADQEAAMA
                     AVKARFAQAAVAVERLDGLSMEFERWRFNLRPSNTEPLLRLNVESRADRGLMELKTKE
                     ILELIGG"
     gene            complement(3709..>4465)
                     /locus_tag="VFX38_01810"
     CDS             complement(3709..>4465)
                     /locus_tag="VFX38_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313632.1"
                     /translation="WKWIDIGLADVVDPNISAAHPFYVRLAISYTNANGTHPSDDFTI
                     TRGYKSYTGGNVEPSDPQLRQYWTPLPCNNIDANNLANIPWSGNSFQGKCPAGPTMAL
                     SAATNIAALTNSDGSPNLTRYYYDQTTGYLYLNVAQDEPNPIGPSPLGSCKPDGTGDA
                     VCPDFEDGETYYVCPKNGCIVYIIEQNDPNYLPGTSVGDPNPAYDKSAPANLNKLVRH
                     GTDTVITREIHLDKQGVPYHTATNAPACTATEP"
BASE COUNT          795 a         1369 c         1494 g          807 t
ORIGIN      
        1 atctacaccg aggcactcag ttccgcgccg ggatcggtcg cgcaacttcg cgaatccacg
       61 gcgctgctcg tgcgctacgc caagcaggcc ggagtcgccg tgtttctcgt cggccacgta
      121 accaagcagg gcgcgattgc gggcccgcgc gtgctcgaac acatggtgga cacggtgctc
      181 tatttcgaaa acgatgccgg cagccgctat cgcgcaatcc gcgccgtgaa gaatcgcttc
      241 ggcgccgcca acgaactcgg cgtcttcgcg atgaccgaga ccgggctcaa ggaagtgcgc
      301 aatccctccg cgattttcct cgcccgcgag ggagcgccga gcgccggaag tgtcgttacg
      361 gtgctgcgcg agggaacgcg cccgctcctg atcgaggtac aggcgctggt cgacgaaagc
      421 cccctcgcca atccgcgccg ggtcgcggtc ggaaccgacg ccaaccgtct cgggatgctg
      481 cttgcggtgc tgcatcgtca cgcgggccgc tcatttcatg gtcacgacgt cttcgtcaac
      541 gtcgtcggcg gactcaaggt cacggaaacc ggaatcgaca tcgcgctcgc cgccgcgctc
      601 gtctccagtc tcgcagacaa accgctgccg gccgacctcg tcgtcttcgg tgaagtcgga
      661 ctcggtggcg aagtacgtcc ggtctacggc ggcgaagagc ggttggccga ggccgctgcc
      721 cagggcttca agcgcgccat cgttcccacc gcgaacgcgc cgcgctcccg gcgcaaaccg
      781 ggtctcgaag tcaaatccgt gcgtagcctc gccgaggcgc tcgccgcact cttttcctga
      841 aggaaacgtg accgcggcaa gccggcggat tgaaagcacc gcaggacatc ttgtcgctgc
      901 aaaaattcca agccgtcgct cgctcttaca tcgatagcgg tggcgacctt tccccgtgaa
      961 gtgcggcgag gccgccagag aggctgcgga cgttgcggta gcctgccgcg agcaggatcc
     1021 gggcggcggt ctggctgcgc agcccgtggg cacagtagac gacgtaggcc cggctcggcg
     1081 gcagttcgat ccgatcggcc tgtgcgagtc gagacagcgg aagatgcagt gcgccggaca
     1141 gatgacccgc atcccattct tcgcgttcgc gcacgtccac gaaaagcgca tcggcgagtc
     1201 gttccagctc ttcggctgcg atgtccgaca ccgacctttc gatgtcctcg agacaaatat
     1261 cttccggtcg gtggcggcag acttcgcagg caggatcgcg tggcagcgcg acaatgcgcg
     1321 tggcgaggct gcggccgtcg aagagccaca ggcgcccgag gatctcgcgc aacgaccgat
     1381 cgtcgccggc ggcgagaatc caccggattg cctcgagcgc ctgcatactt cccgccatgc
     1441 cgagcacggc gccgagtacg cccgcttccg cgcaagtcgc gggcggtatt tccggcggtg
     1501 cggggaaaag gcagcgcagg cagggaccgc gacttgcgtc gaagagcgcg acccgggcct
     1561 cgaagccgag agccgatgcg tgtaccagcg ggcgggcggc cttggcggct gcatcgttga
     1621 tcaggaattt cgtcgccagc cgatcggtcg cgtcgatgac cagatcgcag cgggcgagaa
     1681 gctccatggc attgccggcg tcgaagcgga cgatcttcgg ctcgatgcga atgtccggat
     1741 tgaaggcggc gaggcgttcc gctgcggcct cggccttgtt acgcccgatg tcggcgtcgg
     1801 cgtagagaat ctggcgatga agattggaac gctcgacacg gtcgtcatcg acgatggcga
     1861 gaattccgat tcctgcggcg gcgagatagt acagagcgga actgccgagt ccgccggcgc
     1921 cgatgacgag cacgcgcgcg gcggtgagac gtttctgacc ggccacgccg atttcgggca
     1981 gcagcatctg gcgctgatag cgatccatgg ccgctatttt cccgcgcttt gcccgcccgc
     2041 acaagctccc gcgtcgccat gccgggccgt ttccctataa tggcctgcaa cgattctctg
     2101 gcgagggcac tgcatcgtga acgagaaaac ggggccactc ggccacccga tccgctgctt
     2161 caaggcctac gacgtgcgcg ggcgggttcc cgacgaactc gacgagaaca ttgcctaccg
     2221 gatcgcgcgc gcctgggtgg atttcatcgg tgcgaagcgc gtatgcatgg gtcgcgacgt
     2281 gcgcccgacc agcgacgcct tcgccgacgc ggttgccgcg gggctgatcg actccggggt
     2341 cgaagtgcgc gatctcggta tctgcggaac ggaggaaagt tatttcggca cctttcatca
     2401 gggactcgat ggcggcgtca tgatcacggc gagtcacaac ccgagcgact acaacggtct
     2461 caagttcgtg cgcgagggcg cgcatccgat cagtgccgac aacggcctcg aggacatccg
     2521 cgcgctcgcg gaacgcggcg aattccgcga cgcagacgca aaggggaaac gcgttcccgc
     2581 cgtcgacaag tctgcctacg tcgcacatct tctgagttac gtcgaagtcg aaaagctcaa
     2641 agagctgcgg ctcgtcgtca atcccggaaa cggatgcgcc ggtccgatcg tccaacaatt
     2701 ggagaggagc cttcccttcg aattcgtgaa gatggacttc gagcccgacg ggagtttccc
     2761 caacggcgtg cccaatccga tgctcgagga aaaccgccgc gagacgagcg ccgccgtgcg
     2821 ggcgcacaag gccgatctcg gcattgcctg ggacggggac ttcgatcgct gcttcttcta
     2881 cgacgagcac ggaagtttcg tcgagggcta ctacatcgtc ggacttctgg ccgaagcgtt
     2941 cctgcgccgc aatccgggag cgcgcgtgct gcacgatccg cgcctcacct ggaacacggt
     3001 ggacatcgtg cgcgaattcg gaggaacgcc gatcgtctcg aagagcggcc acgccttcat
     3061 gaaggagaag atgcgcaccg aagactgcat ctacggcggc gagatgagcg cgcatcacta
     3121 cttccgcaaa ttcggctatt gcgacagcgg catgattccc tggctgctgg tggcggggct
     3181 gatttcggcg accggcacgc cgctctcggc gctggtggcc gaccgcgagg cgaagtttcc
     3241 cgcgagcggg gagatcaacc gcgaggttgc cgatcaggag gcggcgatgg cggcggtgaa
     3301 agcgcgcttc gcgcaagccg ccgttgcggt cgaacgtctc gacgggcttt cgatggagtt
     3361 cgagcgctgg cgcttcaacc tgcgcccgtc gaacacggaa ccgctgctgc gactcaatgt
     3421 cgagtcacgc gccgatcgcg gcctgatgga attgaagacg aaagaaattc tcgaactcat
     3481 cggcggctga aagacatccc ccgcgaggac gcggcccctt tcgctccgaa gggggcgtag
     3541 gagattgtca ctgccgaaga aagcggcaag gcacttcccc cttccgaagg aagggggacc
     3601 gagaggggtg tcttttccgt catccgttgc gaggacgcgg ccccctttcc tgcggaagga
     3661 ggcgaggatg cgtcttcctt ccgtggaaag gggggcgagc gagacgtctc agggttcggt
     3721 tgcggtgcac gccggagcgt tggtcgcggt gtgatacggc actccctgct tgtcgagatg
     3781 aatctcgcgg gtgatgacgg tatccgtccc gtgtcgaacg agtttgttga ggtttgccgg
     3841 cgccgatttg tcgtaagcgg gattgggatc cccgaccgag gtgccgggca gataattcgg
     3901 gtcgttctgt tcgatgatgt agacgatgca gccgttcttc ggacagacgt agtaggtttc
     3961 gccgtcttcg aaatccggac agaccgcatc gcccgttccg tccggtttgc aactgccgag
     4021 cggcgagggt ccgatcgggt tcggttcgtc ctgtgccacg ttgaggtaca gatacccggt
     4081 cgtctgatcg taatagtacc tggtgaggtt gggtgaaccg tcggagttcg tgagcgctgc
     4141 gatgttggtc gccgcgctga gcgccatggt cggtccggcc gggcattttc cctgaaaact
     4201 gttgccgctc caggggatgt tggcgagatt gttggcgtcg atgttgttgc aggggagcgg
     4261 cgtccagtat tggcgcaatt gcggatcgct cggttcgacg ttgccgccgg tataggactt
     4321 gtagccgcgg gtgatggtga aatcgtcgga cggatgcgtt ccgttcgcat tggtgtagga
     4381 gatcgcgagg cggacataga acgggtgcgc cgcgctgatg ttcgggtcca ccacatcggc
     4441 gagcccgatg tcgatccatt tccac
//
LOCUS       DASUJG010000093         3067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1614865, whole genome shotgun sequence.
ACCESSION   DASUJG010000093 DASUJG010000000
VERSION     DASUJG010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614865"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VFX38_01815"
     CDS             complement(<1..292)
                     /locus_tag="VFX38_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylargininase"
                     /protein_id="HEX5313633.1"
                     /translation="MSREPAAIALTRAVSPAMARCELTWRERAPLDPARARRQHREYE
                     ETLRALGCRIVALPAEPALPDSVFVEDAAVVTEEIAVITRPGAPSRRGETESI"
     gene            complement(289..1410)
                     /locus_tag="VFX38_01820"
     CDS             complement(289..1410)
                     /locus_tag="VFX38_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX5313634.1"
                     /translation="MRSEQGSPALPAVLERSDLAPQPLAGAWTLPACAYTDEAVAVFE
                     GEAVFARSWQCIGRSDAVREAGAHIVAEVAGRPILAVRGNDGLLRGFFNVCKHRGGPL
                     ALADGCSPRLQCKYHGWTYTLEGQLRSAPEMREADAFRPEDIRLDPVFVGEWQGFVFA
                     AIGAPPADLDATAAGIVGRIAPLRLDGLGLRRRVSYELACNWKLYVDNYLEGYHLPHV
                     HPGLNRLLDCRHYTTELAAWYSDQTSPLRGEGNIYGEGAAHYLYVWPNLMLNVLPGRL
                     QLNLVVPVSVRECRVLFDYYYADGDAAGASERIDADLAFSDEVQREDVAICERVQRGL
                     ESGAYRAGRLSPKREAGVHHFQNLWRAACLRMLGEAQAA"
     gene            1619..1906
                     /locus_tag="VFX38_01825"
     CDS             1619..1906
                     /locus_tag="VFX38_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX5313635.1"
                     /translation="MKLYVGNLPYTTTGEELQGVFAPYGEVASANVVQDRDSGRSRGF
                     GFVEMPNGNEANAAIAGLNGQDFGGRNLVVNEARPRTEGGPRAGGGGFRRR"
     gene            2046..2786
                     /locus_tag="VFX38_01830"
     CDS             2046..2786
                     /locus_tag="VFX38_01830"
                     /inference="COORDINATES: protein motif:HMM:NF025140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4168 domain-containing protein"
                     /protein_id="HEX5313636.1"
                     /translation="MRNHRILRLAVLPAGLALAALAFASPSSSSSQSQAASSTDLSPM
                     PPTRQISDTTLIKFGNAVLAVRGVHREYQPKINAASGSEKDQLQQEEKQAAGAAITPY
                     MPVSQFMQIKSQVRHDPTLRARLKKLVKEHRENTAPPPSGSAPAPVSDATLADFLRAS
                     AALDKVRAQYETKIKAAPDNQTKLSLRKEEKQAAGAAITPYMPVSEYKQVFSQVRHDP
                     QLKARADAMLKEAKKNHHSTTPASASTG"
     gene            complement(2844..>3067)
                     /locus_tag="VFX38_01835"
     CDS             complement(2844..>3067)
                     /locus_tag="VFX38_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX5313637.1"
                     /translation="SAEAVEGMRYLARRHGLFCGPSSGAHLVASKRVRENYPKLETVV
                     TLFCDEGEKYLHDYFMPEASVAVGAKHFT"
BASE COUNT          538 a         1016 c         1019 g          494 t
ORIGIN      
        1 cgatcgactc ggtctcgcct cggcgcgacg gcgcgccggg acgggtaatg acggcgatct
       61 cctcggtgac gacggcggcg tcttccacga acaccgaatc gggaagcgcg ggctccgccg
      121 gcaaggcgac gatgcggcag ccgagcgcgc gaagcgtctc ctcgtattcg cggtgctggc
      181 ggcgggcgcg ggcggggtcg agcggcgccc gctcgcgcca ggtcaattcg caccgcgcca
      241 tcgcggggct caccgcgcgc gtgagcgcga ttgccgcggg ctcccggctc atgccgcctg
      301 cgcttcgccg agcatgcgca ggcaggcggc gcgccagaga ttctggaaat gatgcacgcc
      361 ggcttcgcgt ttgggggaaa gtcttcccgc acggtaggcg ccggattcga gcccgcgctg
      421 cacgcgctcg cagatggcga cgtcttcgcg ttgcacctcg tcgctgaagg cgaggtcggc
      481 gtcgattcgc tcggacgccc ccgccgcgtc gccgtcggcg tagtaataat cgaacaggac
      541 gcggcattcg cggaccgaaa ccggaacgac gaggttcagc tgcaggcggc cgggcagaac
      601 gttcaacatc aggttcggcc atacatataa gtaatgagcg gcgccttccc cgtagatgtt
      661 gccttcgccg cgcagcggac tcgtctgatc cgaataccat gcggcgagtt cggtggtgta
      721 atggcggcaa tcgagcaggc gattgaggcc gggatgcacg tggggcaggt ggtagccctc
      781 gagataattg tccacgtaga gcttccagtt gcaggccagc tcgtagctga cgcgccggcg
      841 aaggccgagt ccgtcgaggc gcagcggggc aatgcgcccg acgatgccgg cggcggtggc
      901 atcgagatcc gccggcggcg cgccgatcgc ggcgaacacg aaaccctgcc attcacccac
      961 gaacacggga tcgagccgga tgtcctccgg gcggaaggcg tccgcctcgc gcatttcggg
     1021 ggcacttcgc agttgccctt cgagagtgta ggtccagccg tgatacttgc attgcagccg
     1081 cggggagcac ccgtcggcga gcgcgagcgg accgccgcga tgtttgcaga cgttgaagaa
     1141 tccgcggagc aggccgtcgt tcccgcgcac ggcgaggatc ggccgtcccg cgacttcggc
     1201 gacgatgtgc gcaccggcct cgcggaccgc atccgagcgc ccgatgcatt gccaactgcg
     1261 ggcgaaaacc gcctcgccct cgaagaccgc cacggcctcg tcggtgtagg cgcaggcagg
     1321 cagggtccag gcgcctgcaa gcggctgcgg cgcaagatcg cttcgctcca gcaccgccgg
     1381 cagggccggg cttccttgct cggaacgcat gctatccacc cgagccgcgg cgtcgcgacg
     1441 agggcgtcgg gcgaggacgc agcggcgggc ggggaagcca tcggtcgcgc cggcgcaaaa
     1501 aactggcgtc gcgccgggtt cccgcgtatg atagccgcac cactgcgcga tctcgtgccg
     1561 tcggtcgctt ccgtggtttg ctttttgacg gcattgttca gtgaggttct agacgaagat
     1621 gaaattgtat gtcggaaatc tcccctatac gacgaccggg gaggaactgc agggtgtctt
     1681 cgccccttat ggcgaagtcg cctctgcgaa cgtggtacag gaccgggatt cgggccgctc
     1741 gcggggcttc ggcttcgtgg aaatgccgaa tggcaacgag gccaatgccg caatcgcggg
     1801 gctgaatggc caggacttcg gcgggcgcaa cctggtcgtg aacgaggctc gcccccgcac
     1861 cgaaggcggg ccacgcgcgg gcggcggagg ttttcgccgc cgctgagccg agccgctatt
     1921 ccggggccgc ctgcgggcgg ccccatccta tccggaactt caaactacgc tccgagtctg
     1981 aatctcgttc gggcgcaagc ggattatttc cgctcgccag tcttaccatc aggagcgttt
     2041 tcactatgcg taatcatcgg atattgaggc tcgccgtgtt gccggccggg cttgcgcttg
     2101 cggcgctggc cttcgccagc ccgtcaagct cctcgagcca gtcacaggcg gcctcctcca
     2161 ccgatctctc gcccatgccg ccgacgcggc agatttccga taccacgctg atcaagttcg
     2221 gcaacgccgt gctcgcggtg cgcggcgtcc accgggaata tcaaccgaag atcaatgccg
     2281 cttcgggaag cgaaaaagat cagctccagc aggaagaaaa gcaggcggcc ggcgccgcga
     2341 tcacgccgta catgccggtg tcgcagttca tgcagatcaa gtcgcaggtt cgccacgatc
     2401 cgaccttgcg ggcgcggctc aagaaacttg tcaaggaaca ccgcgagaat accgctccgc
     2461 cgccgtcggg ttcggccccg gcaccggtga gcgatgcgac gctcgccgac ttcttgcggg
     2521 ccagcgccgc cctggacaag gttcgcgccc aatacgagac caagatcaag gcagcccccg
     2581 acaaccagac caagctctcc ctgcgcaagg aagagaagca ggccgccggc gccgcgatca
     2641 ctccctacat gccggtttcc gaatacaagc aggtgttctc ccaggttcgt cacgacccgc
     2701 aactgaaggc tcgtgccgac gccatgctca aggaagcgaa gaagaatcac cacagcacga
     2761 cccccgcctc cgcaagcacc ggctgaagcc cgtccgcgac gattcgctcc aaggggcgca
     2821 tgatgcgccc cctctttatt ttgtcaggtg aagtgcttcg cgccgacggc aacgctcgcc
     2881 tcgggcatga agtagtcgtg caagtatttc tcgccctcgt cgcagaagag cgtcaccacg
     2941 gtttccagtt tcgggtagtt ctcgcgcacg cgcttggagg cgaccaggtg cgcgccggaa
     3001 ctcggcccgc agaagaggcc gtggcgccgc gcgagataac gcatgccttc gaccgcctcg
     3061 gcgctcg
//
LOCUS       DASUJG010000094         4575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1004270, whole genome shotgun sequence.
ACCESSION   DASUJG010000094 DASUJG010000000
VERSION     DASUJG010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004270"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..576
                     /locus_tag="VFX38_01840"
     CDS             10..576
                     /locus_tag="VFX38_01840"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016915035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX5313638.1"
                     /translation="MRTGSAIAGVDEVGRGPLAGPVVAAAVVLPYGHRLRLADSKLLA
                     PKRREQLDRLIRKRALALAVALATHREIEALGIHHATLLAMARALEALQPAPPGALVD
                     GRFVPESAIPCTPVIRGDASVPAISAASIVAKVTRDAMMFELDHRFPAYGFARNKGYA
                     TAAHLAALASCGPCPEHRRSFAPVRALL"
     gene            582..4043
                     /gene="dnaE"
                     /locus_tag="VFX38_01845"
     CDS             582..4043
                     /gene="dnaE"
                     /locus_tag="VFX38_01845"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006911864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX5313639.1"
                     /translation="MAETFVHLRLHSEYSLVDSVVRIPELMKRTSELEMPAVALTDAG
                     NLFALIKFYRAAEACGIKPIIGADLTLLLEGEERPGLLTVLVCDRGGYRTLCRLITRS
                     YAEGQHGGRPQVNARWFDGGAPGLIALAGRESPIGHAFGAGQARQARERLECLRELFP
                     TGMYLELTRTDIAGEENFNRAAIALALESDTAAVATNNVRFLRRDDYEAHEARVAIHQ
                     GYRLNDPGRERLYSPEQYLKSAAEMAERFADLPAAIAASFAIAERASLELDLGRTILP
                     AFPLPAGEDERSRLRSDAEAGLARRLEGPAFRPAAGADAYRQRLAEELDIIAAMGFAG
                     YFLIVADFIAWAREHGVSVGPGRGSGAGSLVAYALNITDLDPIAYDLLFERFLNPERV
                     SMPDFDVDFGIEGRDRVIDYVAARYGRDHVSQIITFGSMAAKAVVRDVGRVLGHPYGY
                     VDKIAKLVPPRIGIRLEEALKESTELRDLRDSQEDARGLIDLALKLEGLARNAGKHAG
                     GVVIAPAPLSDYAPLYAEANGAVPLTQLDKDDIETIGLVKFDFLGLLTLTIMDRAERI
                     INAERLARSEPPVVARELPLDDRATYALLKSGATTAIFQLESRGMKELIARLQPDCFE
                     DIIALNALFRPGPLQSGMVEDYIARKQGKQKIAYPHPELEGILKSTYGVILYQEQVMQ
                     IARVMAGYTLGSADLLRRAMGKKKPEEMARQREIFMTGSGQRGVAAKTASHVFDLMEK
                     FAGYGFNKSHSAAYALIAYQTAWLKAHYPAAFMAAVLSSDMDHSDKTLRMIDECRRMG
                     LAILPPDVNRSAHFFELEGEQAIRFGLGAIKGLGETATESLVSERRRAGPYRDLADLC
                     ARSDAARLNRRSFEALIEAGALDELGAHRAALLEGLPRALAAAEQKHRAGVAGQVDFF
                     GLAAGAPAAPVAMPERWSARERLAREKRALGLYLSGHPMERYQALLPRLGVQSLGELA
                     AEEEGGERAARQVRIAGLIAELRRFAGRTAVTLDDGSGRMEGVIFADVAARAGRLLLP
                     DHIVVLEGRLGYDDYFNCWRVTVSDIVDMESLIEREAGALWIDWHANGAPPQDFTERL
                     KAVLTTYRGGATVVNIRYRSGGASACLRLGGDWRVAVREDLIAALEGLHGVEQVEVSY
                     QRNAAAY"
     gene            4268..>4575
                     /locus_tag="VFX38_01850"
     CDS             4268..>4575
                     /locus_tag="VFX38_01850"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007039581.1"
                     /note="catalyzes the carboxylation of acetyl-CoA to
                     malonyl-CoA; forms a tetramer composed of two alpha (AccA)
                     and two beta (AccD) subunits; one of the two catalytic
                     subunits that can form the acetyl CoA carboxylase enzyme
                     together with a carrier protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEX5313640.1"
                     /translation="MDLNFLDFEQPIAELEGKIEELRHVSDPSLDLNDEIARLQDKVG
                     QLTQSIFASLSQWQIAQLARHPQRPYFLDYVARVFDDFQELHGDRHYGDDRAILSGLA
                     R"
BASE COUNT          808 a         1465 c         1504 g          798 t
ORIGIN      
        1 acctgagccg tgagaaccgg aagcgcaatc gcgggggtgg atgaagtcgg tcgcgggccg
       61 cttgccggtc cggtggtcgc ggccgccgtc gtgcttccct acgggcatcg tctgcgtctc
      121 gccgactcca agctcctcgc acccaagcgg cgcgagcaac tcgatcggct tatccgcaaa
      181 cgagcgctcg cactcgcggt ggcgctcgcg acgcaccggg aaatcgaggc gctcggcatc
      241 catcatgcga cgctgctagc gatggcacgt gcgctcgaag cattgcagcc cgctccgccg
      301 ggggcgctgg tggacggacg tttcgtgccg gagtccgcca ttccctgcac gcccgtgatc
      361 cggggagatg cgagcgtccc cgcgatcagc gcggcctcga tcgtcgcgaa ggtgacccgc
      421 gatgcgatga tgttcgaact ggatcaccgc tttcccgcct acggttttgc gcgcaacaag
      481 ggatatgcga ctgcggccca tcttgcggcg cttgcgagtt gcggcccctg tcccgaacac
      541 cggcgatcct ttgcgccggt tcgcgccctg ctctgaagac gatggccgag accttcgtgc
      601 atctgcgtct gcacagcgag tactcgctgg tggacagcgt cgtgcgcatt cccgagctga
      661 tgaagcgaac gagcgaactc gaaatgccgg cggtggcgct tacggacgcc ggaaacctct
      721 tcgcactgat caagttttac cgtgccgcgg aagcctgcgg gatcaagccg atcatcgggg
      781 ccgatctcac gctcctgctc gaaggcgaag aacgacccgg cctccttacg gtgctggtct
      841 gcgatcgcgg cggctatcgc acgctctgcc ggctcatcac ccgcagttat gccgaagggc
      901 agcacggcgg acggccacaa gtgaacgcac gctggttcga cggcggcgcg cccgggttga
      961 tcgcgctcgc agggcgggaa agccccatcg ggcatgcgtt cggggcaggc caggcaaggc
     1021 aggcccgcga acgcctcgag tgcctccgtg agctgtttcc gaccggcatg tatctggaac
     1081 tgacgcgcac cgatatcgcc ggggaagaga acttcaaccg tgcggcaatc gcgcttgcgc
     1141 tcgagtccga tacggcggcg gtggccacga acaacgttcg cttcctccgt cgcgacgatt
     1201 acgaagccca cgaggcgcgc gtcgcgatcc atcagggata tcgcctcaac gatcccggcc
     1261 gggaacgttt gtacagtccg gaacagtacc tgaagagcgc ggcggaaatg gccgaacgtt
     1321 ttgcggatct tcccgcggcg atcgcggcga gcttcgcgat cgcggagcgc gcgtcgctcg
     1381 aactcgatct cgggcgcacc attcttccgg cctttccgct tcccgcgggc gaagacgaac
     1441 gttcgcgcct gcgcagtgac gccgaggccg ggctcgcacg ccgactcgaa ggccctgcgt
     1501 ttcgccccgc cgccggagcc gatgcgtatc ggcagcgact tgccgaggaa ctcgacatca
     1561 ttgcggcgat gggcttcgcc ggctatttcc tgatcgtcgc ggacttcatc gcctgggcgc
     1621 gggagcacgg cgtttcggtg gggccggggc gcggttccgg ggcggggtcg ctcgtcgcct
     1681 acgcgctgaa tattaccgac ctcgatccga tcgcctacga cctgctgttc gagcggtttc
     1741 tcaatcccga acgggtgtcc atgccggact tcgacgtcga cttcggcatc gaaggccggg
     1801 atcgcgtcat cgactacgtc gccgcgcgct acggtcgcga tcacgtctcg caaatcatca
     1861 ccttcggcag catggcggcg aaggccgtgg tgcgcgacgt cggaagagtg ctcggtcatc
     1921 cctacgggta cgtggacaag attgcgaagc tcgtgccgcc gcgcatcggg atccggctcg
     1981 aggaggcgct caaggaatcg acggaactgc gcgacctccg tgattcgcag gaagacgcac
     2041 gcggtctcat cgatctcgcg ctcaagctcg aagggctcgc gcgcaacgcc gggaaacacg
     2101 ccggcggcgt cgtgatcgcg cccgcgcccc tcagcgacta cgccccgctc tacgccgagg
     2161 ccaacggtgc agtaccgctg acgcagctcg acaaggacga catcgagacg atcgggctcg
     2221 tgaagttcga cttcctcggc cttctcacgc tcacgatcat ggaccgggcc gagcgcatca
     2281 tcaacgccga gcgcttggcc cgcagcgagc cgccggtcgt ggcgcgagaa ctcccgcttg
     2341 acgaccgcgc cacgtatgcg cttctcaagt cgggagcgac caccgcgatt ttccagctcg
     2401 aatcgcgcgg catgaaggaa ctcatcgccc gtcttcaacc cgattgtttc gaggacatta
     2461 ttgcgctcaa cgcgctcttt cgccccgggc ccttgcaatc gggcatggtc gaggattaca
     2521 tcgcgcgcaa gcaggggaag cagaagatcg cctacccgca tccggaactc gaaggcattc
     2581 tcaagtccac ctacggcgtg attctctatc aggaacaggt aatgcagatc gctcgcgtga
     2641 tggcgggcta cactctcggc agtgccgatc ttctgcgccg ggcgatgggc aagaagaagc
     2701 ccgaggaaat ggcgcgacaa cgtgaaattt tcatgacggg ctcgggacag cgcggagtcg
     2761 cggcaaagac cgccagccat gttttcgatc tcatggagaa gtttgcgggc tacggattca
     2821 acaagtcgca ttcggcggcc tacgcgctga tcgcgtacca gaccgcgtgg ctcaaggcgc
     2881 attaccccgc cgcattcatg gccgcggtgc tttcctccga catggatcac agcgacaaga
     2941 ccctgcgcat gatcgacgaa tgccggcgca tggggcttgc aatcctgcct cccgacgtca
     3001 atcgttccgc tcatttcttc gaactggaag gcgagcaggc gatccgcttc gggctcggcg
     3061 ccatcaaggg tctcggcgag acagccaccg agtcgctcgt ttccgagcgg cggcgtgcgg
     3121 gaccgtaccg tgatctggcg gatctctgcg cgcgcagcga cgccgcccgt ctgaaccgcc
     3181 gctccttcga agccctgatc gaggcgggcg cgctggacga gctgggagcg catcgcgcgg
     3241 cgcttctgga aggattgccg cgggcgctcg cggccgccga gcagaaacat cgcgcggggg
     3301 ttgccggcca ggtggatttc ttcggactcg ccgccggcgc acccgccgca ccggtggcga
     3361 tgccggaacg gtggagcgcg cgggagcgtc tcgcgcgcga aaagcgtgcg ctgggactgt
     3421 atctgagcgg tcacccgatg gaacgctacc aggcgctgct cccgcgtctc ggcgtgcaat
     3481 cactgggaga actggcggcg gaagaagagg gcggcgagcg ggcagcgcgt caggtgcgca
     3541 tcgccgggct catcgcggag ttgcgccgct tcgccggacg cacggccgtc acgctggatg
     3601 acggaagcgg gcggatggaa ggcgtgatct tcgccgacgt cgccgcgcgc gcgggccgcc
     3661 tgctgctgcc cgatcacatc gtggtgctcg aggggcggct cggctacgac gactacttca
     3721 attgctggcg ggtcaccgtc tccgacatcg tcgacatgga aagtctgatc gagcgcgagg
     3781 cgggagcact ctggatcgat tggcatgcga acggcgcgcc cccgcaggat ttcacggaac
     3841 gactcaaggc cgtgctgacg acctaccgcg gaggcgcgac ggtcgtgaac atccggtatc
     3901 gcagcggggg cgcgagcgcc tgcctgcggc ttggcggcga ttggcgcgtc gccgttcgcg
     3961 aggatctgat cgcggcactc gaggggctgc acggcgtgga acaggtggaa gtgagctatc
     4021 agcgcaatgc cgcagcgtat tgaaccgtag ccggacggcg gcttggtttt ggcttgtgaa
     4081 acccgtcgta tctccacctg attggcagag ttaacggtag ccaatacaat aggtaagagc
     4141 gctaacaaaa ggtaaaatct acgatgaatc cgcaggctgt atcgcggcgt ttttcgtctc
     4201 gtgaagggaa tgtgctggag cccgttgcgg ccagcggcta agatggagcg gcagaccgag
     4261 gggacacatg gatctcaatt ttctggattt tgaacaaccg atcgcggaac tcgaaggcaa
     4321 gatcgaggag ttgcgtcacg tgtccgaccc ctcgctcgat ctcaacgacg agatcgcgag
     4381 attgcaggat aaagtcgggc aattgacgca gtccattttt gcttcgctca gccaatggca
     4441 gatcgcgcaa ctcgcgcgcc atccgcagcg tccctatttc ctcgactacg tcgcgcgcgt
     4501 cttcgacgat ttccaggagc tgcatggcga tcgccactac ggcgacgacc gtgcgatcct
     4561 cagtggcctc gcgcg
//
LOCUS       DASUJG010000095         3499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_148196, whole genome shotgun sequence.
ACCESSION   DASUJG010000095 DASUJG010000000
VERSION     DASUJG010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148196"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VFX38_01855"
     CDS             <1..340
                     /locus_tag="VFX38_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313641.1"
                     /translation="LAAAASTIVTEAGVCPDGYAEYDGTLAPGEHVLSPPYQAPAGQE
                     NAILTAPSGFQLFALWKAAGSPWAAYPIPANEVHRWGPAGSYGWVVKALPTSGGGDYT
                     LCIQHPQFGT"
     gene            complement(540..989)
                     /locus_tag="VFX38_01860"
     CDS             complement(540..989)
                     /locus_tag="VFX38_01860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HEX5313642.1"
                     /translation="MRADRVVLDSNVLISALLSPNGSPRRAFEHLAKTSAALLFSDET
                     FAELAGRIALPKFDRYRSIAQMNDCLDWLVELSEWVHPGIAVVACRDSRDDKFLTVAL
                     GGEADILITGDADLLVFDPFEGLPIVSPAKFLKGEAGTDSKGSDRGG"
     gene            complement(979..1230)
                     /locus_tag="VFX38_01865"
     CDS             complement(979..1230)
                     /locus_tag="VFX38_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002739617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX5313643.1"
                     /translation="MKTLSALEAKNRFGQLLNAAQREPVAITRNGRCSVVVLSSEDYE
                     RRRRRAWKNLLRVMNDTGRFAASRGLTEERLARLLSDEG"
     gene            complement(1458..2945)
                     /locus_tag="VFX38_01870"
     CDS             complement(1458..2945)
                     /locus_tag="VFX38_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family protein"
                     /protein_id="HEX5313644.1"
                     /translation="MAEPLILMPDADRPGGDWPRLAMIEPSDYLSRRGAATNHASLVV
                     NLASSYRYLSIGYYSSLLAEARGERVIPSVRTIEDLSRKTIYGLATANFSRFVTRLMR
                     GQSGTDRLRFYSYFGFSPIAGGGELCRQLFERFPAPILRIELGCDKTWRIERLTLVSY
                     QRLPAAEHENFLEGFRRFLGRRWRRSPHAASRYRYDLAVLHDPEEKLPPSDRRALGRL
                     TRAGRRMKVNVELVTRRDYPRIAEYDALFIRETTAINHHSFAFATRAWDEGIPVIDDP
                     DSILRCTNKVYLAELLAKHRIAHPRTLIVSEETLAAAGDELGFPLVLKMPDGSFSRGV
                     FKADDAEALLRIGAELLEDSELVLAQEFLYTPFDWRIGVLAGEPLYACKYFMARKHWQ
                     IYDHSGKRTREGDWETVAIEAAPPEVVDTARRAARLIGGGLYGVDIKELPKRTVVIEI
                     NDNPNLDADVEDACLGDELYERLIAEFIRRIEQPAEALPRRQDPA"
     gene            complement(2938..>3499)
                     /locus_tag="VFX38_01875"
     CDS             complement(2938..>3499)
                     /locus_tag="VFX38_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase C39 family protein"
                     /protein_id="HEX5313645.1"
                     /translation="PELRTDRALEIELWREATTVFMTSGVGGCTPFGLALAAIKRGFA
                     VELYTSAGRSSFADSVRSPLKKEVIRLVQDGFIRELRRLETPMHSGGFALGELMRRMD
                     TGEIPVVLISSYRIYGAKAPHWVVVTGYDERFVYVHDPYVDTAEGETLIDSLNMPILH
                     REFARMKRYGRAGLQAALFVSRPADG"
BASE COUNT          636 a         1049 c         1192 g          622 t
ORIGIN      
        1 gctcgccgcc gccgcgtcga cgatcgtcac cgaagccggc gtctgtccgg acggctacgc
       61 cgaatacgac ggcacgctcg cgcccggtga acacgtcttg tcgcccccct atcaggctcc
      121 ggccgggcag gagaacgcga ttctcacggc gccctcgggc ttccagctct tcgccctgtg
      181 gaaagctgcg ggctctccct gggcggcgta tcccatcccg gcgaacgaag ttcaccgttg
      241 gggaccggcc ggaagctatg gctgggtggt gaaggcactg ccgacgagcg gcggcggcga
      301 ttacaccctg tgcatccagc atccgcaatt cggtacgtag gatgtggtga gtcctcggac
      361 cgcatcgatg aacttgcaag caacggcgcg gttcgagggc tcgccgcatc cggcggagcc
      421 gattcctgtg acgccccgga cgctgaagcc ggagcggcat agcggccggc aatcggccgc
      481 agtgggtcga cattcgaatt ggcgaggtcg ctgtgaagag cggcggcctc gccgtcttgt
      541 cagccgccgc ggtcgcttcc ttttgaatcg gtgccggctt cgcctttgag aaacttcgcg
      601 ggcgaaacga tgggcaagcc ctcgaagggg tcgaagacga gcagatcggc gtcgcccgtg
      661 atcaggatgt ctgcttcgcc ccccagcgcg acggtaagaa atttatcgtc gcgggagtcg
      721 cggcaagcga cgactgcgat acccggatgc acccactcgc tcaactcgac cagccagtcc
      781 aggcagtcgt tcatttgcgc gatactgcga tagcggtcga atttgggaag ggcgatgcgg
      841 cctgcgagtt cggcgaatgt ctcatcggaa aacaacaatg ccgcggatgt tttcgccaag
      901 tgctcgaacg cccgcctcgg cgagccattc ggactcaaca gtgcgctgat cagaacgttg
      961 ctgtcgagga caacgcgatc agccctcatc ggacagcagt cgggcaagcc gctcttcggt
     1021 caatcctcgg gaggccgcga aacgcccggt atcgttcatg acccggagca gattcttcca
     1081 ggcgcggcgg cggcgtcgct catagtcctc gctggacaac acgaccacgc tgcatcgccc
     1141 gttcctggtg atcgcgacgg gctcgcgctg ggcggcattg agcaattggc cgaatcggtt
     1201 cttggcctcg agggcggaca gggtcttcat gaggatcgct cgaatttgac atgcctgtaa
     1261 tagctaaaac agctataaac ctcaatcgcc tcgagcagca gaaccgggct tggccgttac
     1321 cggcgcaagc gcagtttgcg cgcggctgcc gccgacagct tggattcgcc gccgcgatcg
     1381 agtctggatg gagaatcgca aacaccctgt cgcggatcgc cgcttccacg gaaagaatgg
     1441 cgcctcgcga cgccgggcta tgcgggatcc tgccggcgcg gtaatgcttc ggcaggctgc
     1501 tcaatgcggc ggatgaattc ggcgatcaac ctttcgtaga gctcgtcgcc gaggcaggcg
     1561 tcttcgacat cggcgtcgag attcgggttg tcgttgattt cgatcactac cgtgcgtttg
     1621 ggcaattcct tgatgtcgac gccgtaaagt ccgcctccga tcaagcgcgc ggcgcgccgc
     1681 gcggtgtcca ccacttcggg gggagcagcc tcgatggcaa cggtttccca gtcgccctcg
     1741 cgcgtgcgct tgccggagtg atcgtaaatc tgccaatgct tgcgggccat gaagtacttg
     1801 caggcgtaga gcggttcgcc ggcgagcacg ccgattcgcc agtcgaaggg cgtatagaga
     1861 aattcctgcg ccagcacgag ttcggagtcc tcgagcagtt cggcgccgat gcgcaggagc
     1921 gcctcggcgt cgtcggcctt gaacacgccg cgcgagaagg aaccgtccgg catcttgagc
     1981 acgagcggga agccgagttc gtctcccgcc gcggcgagcg tttcctcgct cacgatcagg
     2041 gtgcgcggat gggcgatgcg gtgtttcgcg agcagttcgg cgagatacac cttgttggtg
     2101 cagcggagaa tcgagtcggg atcgtcgatg accgggatgc cttcgtccca tgcgcgtgtc
     2161 gcgaaggcga agctgtgatg gttgatggcc gtcgtctcgc ggatgaagag cgcatcgtat
     2221 tcggcgatcc ggggataatc gcggcgggtg acgagttcca cgttgacctt catgcgccgt
     2281 ccggcgcgcg tgagccggcc gagcgcgcga cgatcggaag gcgggagttt ttcctccgga
     2341 tcgtggagca cggcaagatc gtagcgatag cggctggcag cgtgggggct gcgccgccag
     2401 cgccgcccga gaaagcggcg aaagccttcg agaaagtttt cgtgttcggc ggcgggcagg
     2461 cgctggtagg aaacgagcgt gaggcgttcg atccgccagg tcttgtcgca accgagttcg
     2521 atgcgcagga tcggcgccgg aaagcgctcg aaaagctggc ggcagagttc gccgccgccg
     2581 gcgatcgggc tgaagccgaa gtagctgtag aagcgcagcc ggtcggtgcc gctctgcccg
     2641 cgcatgaggc gtgtcacgaa gcgcgagaaa ttcgcggtgg cgagtccgta gatggtcttg
     2701 cgcgagagat cttcgatcgt gcgcacgctc ggaatgaccc gttcgccgcg cgcctcggcg
     2761 agaagcgagc tgtagtagcc gatcgaaaga taacgatagc tcgaggcgag gttgacgacg
     2821 agcgaggcgt ggttggtcgc cgctccgcgc cgcgaaagat agtccgaggg ctcgatcatc
     2881 gccaaacgcg gccagtctcc gccgggacga tcggcatcgg gcatgaggat gagcggttca
     2941 gccatcggca ggccgcgaga cgaagagggc tgcctgaagg ccggcgcgac cgtagcgttt
     3001 catgcgcgcg aattcgcggt gcaggatcgg catgttgagc gaatcgatga gcgtttcgcc
     3061 ctcggccgtg tcgacatagg gatcatgcac ataaacgaaa cgctcgtcgt aaccggtgac
     3121 gaccacccag tgcggggcct tggcgccgta gatgcgatac gaactgatca gcacgaccgg
     3181 aatctcgccc gtgtccatgc gtcgcatgag ttcgccgagc gcaaagccgc cgctgtgcat
     3241 cggagtctcg aggcggcgaa gttcgcggat gaagccatcc tgcacgagac ggatcacttc
     3301 ctttttgagc ggactgcgca cggaatcggc gaaggaactg cgtcccgcgg aggtatagag
     3361 ttcgacggcg aagccgcgtt tgatcgccgc gagcgcgagg ccgaagggcg tgcagccgcc
     3421 gacgcccgaa gtcatgaaca cggtggtcgc ctcgcgccac agttcgattt cgagcgcgcg
     3481 gtcggtcctg agttcgggc
//
LOCUS       DASUJG010000096         2228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_882335, whole genome shotgun sequence.
ACCESSION   DASUJG010000096 DASUJG010000000
VERSION     DASUJG010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882335"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..1615)
                     /locus_tag="VFX38_01880"
     CDS             complement(2..1615)
                     /locus_tag="VFX38_01880"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5313646.1"
                     /translation="MYAEFFGLAEAPFSIAPDPRYLFLSPQHREALAHLLYGTTEGNG
                     FIQLTGEVGTGKTTLVRRLLAELPEGVEIAFIFNPELSRQGLLRAVLGELGIPLPPAD
                     ADRGVLAERLYRYLIGAHARGRRVVLVIDEAQRLSPEVLEEVRLLTNFETDRRKLLNV
                     ILVGQPELRTTLARADLRQLAQRITARYHLGPLGVVETDEYIRHRLRLAGAPGRIFRP
                     QAVRAIARASHGIPRLINLICDRALMAAYARDLREVSAPLVREAVSDLAGVRRRAPRD
                     PRPYYYAAGFVVALVAGAALAWWLSAGPRGSPGPIAAVAAAKDPVQARAMTAVAETVK
                     AAPVATAGNPIMDWAASAKPDVGQATAALLTLWGIPASDAPDPCAAAIAAGLACLDEQ
                     GNLNTLRNFNRPTVVTLTLGARSVSAVVQSLDAGKVTLALAPGLARSFSLGDFESLWY
                     GRMWLLWRPPEGANVIQFGMRGPAVSWLRHALGVDAAAGTPFDEALEKAVREFQGKHA
                     LVADGIAGPETLIVLNRIHNPESGPRLRG"
     gene            complement(1631..2182)
                     /locus_tag="VFX38_01885"
     CDS             complement(1631..2182)
                     /locus_tag="VFX38_01885"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HEX5313647.1"
                     /translation="MKSFRRLLLPALGLLLIGGCAAPVQYEYNSAPYEQWHNFAWVAP
                     PSAPIRNPVVDSGILATRVEQAVIATLTNQGYYQVATPAQADFVVTYHTALERQQVPN
                     PSVGFSYGWWGYPFSTVIVSQPGQREIQQADLIIDVASAKTKQLVWRGWVTNSPSRSN
                     YSQQAVNEAVAEILSKFPPPPKF"
BASE COUNT          387 a          739 c          761 g          341 t
ORIGIN      
        1 ttcagccgcg cagacgcggc cccgattccg gattgtggat gcggttgagc acgatcagcg
       61 tttccggacc cgcgattccg tcggcgacga gcgcatgctt gccctgaaac tcgcgcacgg
      121 ccttctcgag cgcctcgtcg aacggtgtcc ccgcggccgc atcgactccc agggcatggc
      181 gcaaccagct cacggccggc ccgcgcatcc cgaactgaat gacgttcgcg ccttcgggag
      241 gacgccacag aagccacatg cgcccgtacc agagcgattc gaaatcgccc agagaaaagg
      301 accgcgcgag acccggagcg agcgcgagcg tcaccttgcc ggcatccagc gactggacga
      361 ccgccgagac gctgcgggcc ccgagcgtca gcgtgacgac ggttggccgg ttgaaattgc
      421 gcagggtgtt gagattcccc tgttcgtcga gacaggcgag cccggccgcg atcgccgcgg
      481 cgcaggggtc gggcgcatcg ctcgccggaa tgccccaaag cgtgagcagc gccgccgtcg
      541 cctgcccgac atcgggcttg gcgctcgccg cccagtccat gatcggattg cccgccgtcg
      601 ccaccggggc cgctttgacc gtttccgcga ccgcggtcat ggcccgagcc tgcacgggat
      661 ccttggccgc ggccactgcc gcgatcggac ccggacttcc ccgcgggccg gcgctgagcc
      721 accaggcgag cgcggctccg gcgacgagag caacgacaaa gcccgccgca tagtagtaag
      781 gccgcggatc gcggggcgcg cgacgacgca ccccggccag atcgctcacc gcctcgcgca
      841 ccagcggagc ggagacctcg cgcaggtcgc gcgcgtaggc cgccatcagg gcgcgatcgc
      901 aaatcagatt gatcagccgc ggtattccgt gggaggcgcg ggcgatcgcg cgcaccgcct
      961 gcgggcggaa aatccgtccc ggagcgccgg cgagacgcag ccggtggcgg atgtactcgt
     1021 cggtttcgac gacgccgagc gggcccaggt gatagcgcgc cgtgatccgt tgcgcgagct
     1081 ggcgaagatc ggcgcgcgcg agcgtggtac gcagttccgg ctggccgacg agaatcacgt
     1141 tgagaagctt gcgccgatcg gtttcgaagt tggtgagaag gcgcacctct tcgagcacct
     1201 ccgggctcaa ccgctgcgcc tcgtcgatca cgagcacgac gcggcggccg cgcgcatgcg
     1261 ccccgatgag atagcgatac agccgctcgg ccagtacgcc gcgatcggcg tccgcgggcg
     1321 gcaacggaat cccgagttcg cccagcaccg cgcgcaggag tccctggcgc gagagctcgg
     1381 gattgaagat gaaggcgatt tccacgccct cgggaagttc cgcgagcaag cggcgcacga
     1441 gggtcgtctt gccggttccg acctcgcccg tgagctggat gaatccgttg ccttccgtcg
     1501 taccgtacag caggtgcgca agggcctcgc gatgctgcgg gctcaggaaa agatagcgcg
     1561 gatcgggcgc gatcgaaaac ggcgcttccg ccagaccgaa aaactcggcg tacacgatcc
     1621 ggggcgttcc tcagaatttc ggaggcggcg gaaacttcga caatatctcg gcgaccgctt
     1681 cgttcacggc ctgctgcgag taattgctgc gactggggga attcgtcacc cagccgcgcc
     1741 acacgagctg tttcgttttc gcgctggcga catcgatgat gagatccgcc tgctgaattt
     1801 cgcgctgccc gggctggctc acgatgacgg tgctgaaggg atagccccac cagccgtacg
     1861 agaagccgac cgagggattc ggaacctgct ggcgctcgag cgcggtgtga taggtcacca
     1921 cgaagtccgc ctgcgcgggg gtcgctacct ggtagtagcc ctggttcgtc aaggtcgcga
     1981 tcaccgcctg ttccacgcgc gtggcgagaa tgccgctgtc caccacggga ttgcggatcg
     2041 gcgcggacgg cggcgcaacc caggcgaaat tgtgccattg ctcgtacggg gcgctgttgt
     2101 attcgtactg caccggagcg gcgcagcccc cgatcagcag gagcccgagt gcgggcagga
     2161 ggagtcggcg gaaggatttc atcgagtatc ccgaacggcg gtcgtgtatc tgctattgca
     2221 tcactaaa
//
LOCUS       DASUJG010000097         8572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_26767, whole genome shotgun sequence.
ACCESSION   DASUJG010000097 DASUJG010000000
VERSION     DASUJG010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8572
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26767"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VFX38_01890"
     CDS             complement(<1..303)
                     /locus_tag="VFX38_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313648.1"
                     /translation="MLFALLSSLALSFSAAAAMAAPAHRGFAERDIATRVHTMLTPQQ
                     IAAFLPERGAFTFPAPWGTRGVRITNASDCGGQDCLDNIYTYWRNLSNSAGSNTMYI"
     gene            complement(533..1165)
                     /locus_tag="VFX38_01895"
     CDS             complement(533..1165)
                     /locus_tag="VFX38_01895"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5313649.1"
                     /translation="MMPRVLLADDHPMFREGLRMLLEREDFEIVAECADGNGAVASAR
                     ALRPDAAVLDIAMAGLNGLDAARQIRDEATSRVILLSAYEDAAYLRAAREAGVFGYVL
                     KAQAGSELTEVLHRALRMPLGGSRAATRAGSRLIGPDAMQECARPLTQRESQILCLIA
                     NGNTTRETATILGISVKTADSHRSHLMQKLDIHETAGLVRYAVREGMIRA"
     gene            complement(1162..2223)
                     /locus_tag="VFX38_01900"
     CDS             complement(1162..2223)
                     /locus_tag="VFX38_01900"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5313650.1"
                     /translation="MASVGAAPEVEGDGGALAADFEGTGERSRTRRSTGATLRVADGA
                     DATLAARYRNRLRDYLHGAATASLEDAQALGREALASESGILAWLATHREAAGALGVN
                     RLATEDAVRKAAERFFAASLASFDRAHREHRKSQDALRRINGCLENEARRIALLLHEE
                     AAQLLAAAYLELAGIERETAMAAIKPRAARMRAQLDGVRGQLRHISHELSPPALERLG
                     LIPALEFLAEGVHERSALDIAIDASSVCETRLSPPIETTLYRAVQEALNNAVQHAAAT
                     HARVTLRCDRDRVICTIADDGRGFVRSPGGKQATAGGLGLIAIRARLQSLGGSLHIAS
                     VPERGTTLRISVPLAGNAR"
     gene            complement(2211..2720)
                     /locus_tag="VFX38_01905"
     CDS             complement(2211..2720)
                     /locus_tag="VFX38_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5313651.1"
                     /translation="MNELPALPLGYTYPANHNPLTDSRPPPREPIRLWSRAARGNRIA
                     VAAMNDIVTARQYGGELARKCGFSSNDTIIIVTIISELARNIVLYAGRGDITVSLNAS
                     DERCGISIVGRDEGPGIADTERATAGGYSTSGGLGLGLAGVRGMADDFRIDSRPGGGT
                     VVTVEKWLP"
     gene            2914..3672
                     /locus_tag="VFX38_01910"
     CDS             2914..3672
                     /locus_tag="VFX38_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5313652.1"
                     /translation="MERVLSHEQSKALNGSFTALVATGDPAIAAVVQPLLVRLHFEVR
                     TVADDGSLADWLGADEPDMLLLDNGLPPAGGLEVCRRLRDQPRTARLPIIVMATRGNG
                     SATIQALEAGADDCIGTPWEADAIAARIKALARRALVAPAGEHLRAGPIDMDLARWTA
                     SVNRAPVDLTKTEFKLLRALLEARGRTLTREQLLRMVWSHANAYGLNTRTVDVHMGRL
                     RRKLGFAGPFIITVRNVGFRFEIMADWIAEGRAS"
     gene            complement(3734..4333)
                     /locus_tag="VFX38_01915"
     CDS             complement(3734..4333)
                     /locus_tag="VFX38_01915"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5313653.1"
                     /translation="MSQTKFIGIVSLATALVLGSLGALAAGIPSPAQSIGSTGDEGAL
                     ALLERNAAAGDHVAQFQLGQKYAFGQGTTQNYAKAAQWWQKAAASGDPVAQTDLGWLY
                     YQGQGVPQDYRQAAAWWRKGAEAGNAKAQGGLAWLYATGQGVGRHNVTAAKWAIVAKA
                     GGFDRASGVLQYVESQMSRGQIREAQRQADSWWAAHRAD"
     gene            complement(4428..4503)
                     /locus_tag="VFX38_01920"
     tRNA            complement(4428..4503)
                     /locus_tag="VFX38_01920"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4468..4470),aa:Thr,seq:cgt)
     gene            complement(4530..4937)
                     /locus_tag="VFX38_01925"
     CDS             complement(4530..4937)
                     /locus_tag="VFX38_01925"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEX5313654.1"
                     /translation="MSSRPLAEQLRRLGIAPTPQRLRIAAVMLAAPRHFRAEEVLEEV
                     NREPPGVSKATLYNSLRLFTARGLLREVIVDPERVLYDSTAQAHHHFYDPATGELTDI
                     PAECLTVSGLPEPPPGTEAGEIEVLVRLRPAGC"
     gene            5111..6505
                     /locus_tag="VFX38_01930"
     CDS             5111..6505
                     /locus_tag="VFX38_01930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX5313655.1"
                     /translation="MRPVLLPMNAEAIAPIAWRALAARIVAIALPVIANNLLTVGMQI
                     TDAVLAGRLSPQTLAAVSVGGGVYVPFFLFALGILAALSPTSAQLYGAGRLHDIGVAA
                     RDASWLALVLAALLMSGFRFLDPLFRVAGINVALAPAAGAYVAALSWGLPAIFLYLVL
                     RFASEGIGHTRPMLYVALAAFLVNIPLDYWFMFGGLGVPRLGAAGCGYATALAQWLAL
                     AVMLLYVRVCRRRYAPLRIFAPMRRPQFEGIAALLRLGLPIGVMLFAEVGLFGAAALL
                     MASFGTVTAAAHQVAVAVASFAFMVPMGLATAISVVVGQAAGAGDPRNARRAGMVGIA
                     LSLGFEIASAILMFVLREPLARLFTADPAVVALAAKLLALAAAFQLSDGLQVASAGAL
                     RGLKDTRAPMFITLVAYWGSGFPLAWAFAFPLGLGPAGVWVGLIAGLTVAGVLLFLRY
                     ARRSRPDAAAMIRA"
     gene            6502..6840
                     /locus_tag="VFX38_01935"
     CDS             6502..6840
                     /locus_tag="VFX38_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5313656.1"
                     /translation="MNLDERLQGLLGQGHDGAPLRVSLALACLGRGDPANAIVHLERA
                     LALDPDYSAAWKTYGRALLTDARDEEARAAWERGIAAARAAGDRQSEREMQVFLRRLD
                     PLDRQQKEPT"
     gene            6837..7568
                     /locus_tag="VFX38_01940"
     CDS             6837..7568
                     /locus_tag="VFX38_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018950930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX5313657.1"
                     /translation="MNDPFGLPGADVRKAPADESLLSREAWKVFQIMAEFVEGYERLA
                     RIRPSVSIFGSARVAPEHPYYRLGEEIGFELSRAGFSVVTGGGGGLMEAANKGAFRGK
                     APSIGLNIRLPKEQKANVWQDVNITFQHFFSRKVMFVKYASAYVILPGGFGTLDEFSE
                     ILTLIQTGKSRRIPIILVGGEYWRGLLDWFRDRLVPEQMIAAEDIDLVSLAEEPRQVV
                     ELIFEHYGERGFEPSPEEREKMLEL"
     gene            7580..8056
                     /gene="dtd"
                     /locus_tag="VFX38_01945"
     CDS             7580..8056
                     /gene="dtd"
                     /locus_tag="VFX38_01945"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019023482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX5313658.1"
                     /translation="MIALLQRVSEASVSVGGETVGTIGAGLAVFVCVERDDGEAQAAR
                     LVERLLGYRVFAGEGGKLNRSLAETGGGLLLVPQFTLAADTRKGTRPSLGRAALPEQG
                     RALFERVLALARASHPLVASGVFGARMQVRLVNEGPVTFWLEARAGVSCEPGRGTE"
     gene            8062..8244
                     /locus_tag="VFX38_01950"
     CDS             8062..8244
                     /locus_tag="VFX38_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatA"
                     /protein_id="HEX5313659.1"
                     /translation="MGLGGISVWQLILILVIVVCTLLAVPLVHGGDAPPSPKKEEDND
                     TPEEDEPEEDPPLGVG"
     gene            complement(8330..>8572)
                     /locus_tag="VFX38_01955"
     CDS             complement(8330..>8572)
                     /locus_tag="VFX38_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEX5313660.1"
                     /translation="LRLVDAYRWSGSRRISMRDDVIDAVYLFHQALGGRARYAAQPAE
                     VKTICLGLKRSLILRHFPTATALRHHLETFDWGTRG"
BASE COUNT         1303 a         2791 c         2905 g         1573 t
ORIGIN      
        1 aatgtacatc gtgttgctgc cggcgctgtt gctcagattg cgccagtagg tgtagatgtt
       61 atcgaggcag tcctggccgc cgcagtcgct cgcattggtg atgcgcaccc cgcgtgtccc
      121 ccagggtgcg ggaaaggtga acgccccgcg ctcgggcagg aacgccgcaa tctgctgcgg
      181 cgtcagcatc gtgtgtacgc gggtcgcaat atcgcgttcg gcgaagccgc gatgggcagg
      241 agccgccatc gccgcagccg cagagaacga caaggccagg gaagaaagga gcgcgaacag
      301 cacgctccgg gaacggatgg gagagaaaga caattgcatg ggaatccgga gtttgaggaa
      361 tcaagctccg gatgttaccc gaatcggcgc gccgatttgc cctaattgtg taacgaaggt
      421 ttgcagaaac gagaaagtcg ccgcaacgat ttcaaaacag gctttggatc aaggggttcc
      481 gccggccttg tggggacgtt ttttgcgcgg cgcggcgcca ttcgcgccgg catcaggcgc
      541 gaatcatgcc ttcgcggact gcgtagcgga ccaggcccgc ggtttcgtgg atatcgagct
      601 tctgcatcag gtgactgcgg tgcgagtctg ccgttttcac gctgatgccg aggatcgtcg
      661 cggtttcccg cgtggtgttg ccgttcgcga tcaggcacag aatctgggat tcgcgctgcg
      721 taagggggcg tgcgcattcc tgcatcgcgt ccggaccgat cagacgggac cctgcgcgcg
      781 tcgccgcgcg cgaaccgccg aggggcatac gcaaggcccg gtgcagcacc tcggtcaact
      841 cggatcccgc ctgcgccttg agcacgtagc cgaagacgcc ggcctcgcgc gcagcacgca
      901 gataggcggc atcttcgtag gcgctgagga ggatgactcg tgatgtcgcc tcgtcacgga
      961 tctgtctcgc ggcatcgagc ccgttcagcc ccgccatcgc gatgtcgagc accgccgcgt
     1021 cgggacggag cgcgcgcgcg gaggcgacgg cgccgttgcc gtcggcacat tcggcgacga
     1081 tttcgaaatc ctcccgctcg aggagcatcc tcaggccctc gcgaaacatc ggatggtcgt
     1141 cggcgagaag aacccgcggc atcatcgcgc gtttcccgcc agcggaaccg agattcgcaa
     1201 ggtcgttccg cgttccggca cggaggcgat gtgcaggctg ccgccgagac tctgcaatcg
     1261 ggcgcggatt gcgatcaggc ccaatcctcc cgcggtggct tgcttgccgc cggggctgcg
     1321 gacgaatccg cgtccgtcgt ccgcgatcgt gcagatcacg cggtcgcgat cgcaacgcag
     1381 cgtgacccgc gcgtgggttg cggcggcgtg ttggactgcg ttgttgagtg cctcctgcac
     1441 cgcccggtag agggtcgtct cgatgggcgg cgacaatcgg gtttcgcaga cgctcgaggc
     1501 gtcgattgcg atgtcgagcg cggagcgttc gtgaacgccc tcggcgagaa actcgagcgc
     1561 cggaatcagc cccagtcgtt cgagcgcggg cgggctgagt tcgtgggaga tatggcgaag
     1621 ctgcccgcgt actccgtcga gctgcgcgcg catgcgcgcg gcccgcggct tgatcgcggc
     1681 catcgcggtt tcccgctcga ttccggcgag ttccaggtac gctgctgcca gcagttgcgc
     1741 cgcctcctcg tgcagcagaa gggcgatgcg ccgggcctcg ttttcgagac agccgttgat
     1801 tcggcgcaac gcatcctgcg acttgcgatg ttcgcgatgc gcacgatcga acgaggcgag
     1861 gcttgcggcg aaaaatcgtt cggctgcctt gcggacggca tcttccgtcg cgagccgatt
     1921 gacgccgagc gcgccggccg cttcgcgatg cgtcgccaac cacgcgagga ttccggattc
     1981 gctcgcgaga gcctcacgcc ccaacgcctg cgcgtcctcc agggacgctg tagcggcgcc
     2041 gtggagatag tcccgcaagc ggtttcgata gcgggcggct agcgtcgcgt cggcgccgtc
     2101 tgccacccgc agcgtcgccc ccgtgcttct ccgtgtacgc gatcgttcgc ccgtgccttc
     2161 gaagtccgcc gcgagagctc cgccgtcgcc ttccacttcg ggcgcggcgc ctacggaagc
     2221 catttttcca ccgtcacgac ggtgcctccc ccggggcggc tgtcgatgcg gaaatcgtcg
     2281 gccatgccgc gcacgccggc gagtcccaat ccgaggccgc ccgaggtcga atagccgccc
     2341 gcggtcgcgc gctcggtgtc ggcgatgccg ggtccctcgt cgcggccgac gatcgagatg
     2401 ccgcagcgtt cgtccgatgc attcagggaa acggtgatgt cgccgcgtcc ggcgtagaga
     2461 acgatgttgc gggcgagttc cgagatgatc gtgacgatga tgatcgtgtc gttgctggag
     2521 aatccgcatt tgcgcgcgag ttctcctccg tattgccgcg ccgtcacgat gtcgttcatc
     2581 gcggccacgg cgatgcggtt cccgcgggcc gctcgcgacc acaggcggat cggctctcgc
     2641 ggcggcggcc tcgaatcggt gagcggattg tggtttgcgg gataggtgta tccgagcgga
     2701 agagcgggaa gttcgttcat gtcttgcaac ccttcgcatg ccaatgctgt aggacattag
     2761 cagctcgcgg cgccggaaac tgttcgctgg cgcacggatc acgatccgct tctcactcgt
     2821 ttcgacgtgg gggccgatgg ttcgcggcgc gaacgtgcgc gaacgcacgt tcggcgttcc
     2881 cgcgccgtta ccgatcgcgc tagtatgcag acaatggagc gggtactttc acatgagcaa
     2941 tcgaaggcgt tgaacgggag ttttacagca ttggtagcga cgggtgatcc ggcgatcgcg
     3001 gccgtagtgc agccgttgct cgtgcgactg catttcgagg tgcgcacggt cgccgatgac
     3061 ggcagccttg ccgactggct cggcgcggac gagcccgaca tgcttcttct cgacaatggc
     3121 ctgcctccgg cgggagggct cgaagtctgt cggcggctcc gtgatcagcc gcgcacggcg
     3181 cgcctgccga tcatcgtcat ggcgacccgc ggcaacggaa gcgccacgat ccaggcgctc
     3241 gaggcgggag cggacgactg catcggcacg ccctgggagg ccgacgcgat tgccgcgcgc
     3301 atcaaggcgc tcgcacggcg agcgctcgtc gcgcccgccg gcgagcactt gcgcgccggg
     3361 ccgatcgaca tggatctcgc gcgctggacc gcctcggtga atcgtgcgcc ggtggatctg
     3421 accaagaccg agttcaagct gctgcgggct ctcctcgagg cgcgcggcag gacgctcacc
     3481 cgcgaacaac ttctgcggat ggtgtggtcg cacgcgaacg cctacggcct caatacccga
     3541 acggtggacg tgcatatggg acggttgcgg cgcaaactcg gtttcgccgg accgttcatc
     3601 atcacggtgc gcaacgtggg gtttcgcttc gagatcatgg cggactggat tgccgaggga
     3661 cgcgcgtcct gaaatcaatc gccgcgcggg cgtctgtcgc gttcccgacg cgcggtcgcg
     3721 ggcgagccgg cgatcagtct gcgcggtggg ccgcccacca ggagtccgcc tgacgctgcg
     3781 cctcgcggat ctggccgcgc gacatctgcg actccacgta ttgcagcacc cccgaggcgc
     3841 gatcgaatcc gcccgctttg gcgacgatgg cccacttcgc cgcggttacg ttatgtcggc
     3901 ccacgccctg cccggtggcg taaagccagg cgaggccgcc ctgtgccttc gcgtttccgg
     3961 cttcggcgcc cttgcgccac catgcggccg cctggcgata gtcctgggga acgccctggc
     4021 cctgatagta aagccagccg agatcggtct gcgcgaccgg atccccgctc gcggctgcct
     4081 tctgccacca ctgtgcggcc ttcgcgtagt tctgcgtcgt tccctgaccg aaggcgtatt
     4141 tctgtcccag ttggaactgc gcgacgtgat ctcccgccgc tgcgttgcgt tcgagcagcg
     4201 cgagtgctcc ctcgtcgccc gtgctgccaa tcgattgcgc gggcgagggg attccggccg
     4261 ccagcgcgcc aagcgaaccg aggaccagcg ccgtcgccag cgacacgatt ccgatgaatt
     4321 tcgtctggct catgacgcgc tcctgcgatt cgtatgacga aaatatagcg cagattctcg
     4381 caatgaaatg gcggcaaaac gcggcagcaa gcgtttgcag gtagaactgg tgccggaagc
     4441 aggagtcgaa cccgcgacct tcgcattacg aatgcgctgc tctaccgtct gagctattcc
     4501 ggcacgcggc aaattctaac ccacggtctt caacagcctg ccggacgaag gcgcacgagc
     4561 acttcgattt cgccggcctc ggttccgggc ggaggctcgg ggagtccgct gacggtgagg
     4621 cattcggccg ggatgtcggt gagttcgccg gtggcggggt cgtaaaagtg atggtgcgcc
     4681 tgcgcggtcg agtcgtagag cacgcgctcg gggtccacga tgacttcgcg cagcaggccg
     4741 cgtgcggtaa acagacgcag gctgttgtag agcgtcgcct tggaaacgcc gggcggttcg
     4801 cgattgactt cctccagcac ttcctcggcg cgaaagtgcc gcggcgccgc cagcattacc
     4861 gcggcgatac gcaggcgctg cggcgtgggg gcgatgccga gacgccgtaa ctgttcggcg
     4921 aggggccggc tgctcatgtc cctctccggt tgcgaccggg actcattata agcgacggat
     4981 gcgcggcgaa ccgatgcgcg ctcgctccgt cgctcggcag gcgaggaggc gaatcggccg
     5041 gcattgcgct ccgggcaaaa cagatggcga gcaccgggag tgcgtcgaag cttcccgtat
     5101 catccgcagc gtgcgccccg tactcctgcc catgaacgcc gaagcgatcg cgccgatcgc
     5161 ttggcgcgcg ctcgccgcgc gcatcgttgc gatcgcgctt cccgtgatcg ccaacaatct
     5221 gctcacggtc ggcatgcaga tcaccgatgc cgtgctcgcc gggcggctgt cgccgcagac
     5281 cctcgccgcg gtatccgtgg gcggcggcgt gtatgtgccg ttctttctct tcgctctcgg
     5341 aattctcgcc gcgctcagtc cgacgagcgc gcagctctat ggcgccggcc gcctgcatga
     5401 catcggcgtc gcggcacgag atgcgagctg gctcgcgctc gtgctcgcgg ccctgctcat
     5461 gagcggattc cgtttcctcg atccgctctt tcgcgtcgcc gggatcaacg ttgcattggc
     5521 gcccgccgcg ggcgcctacg tcgccgcgct gtcctgggga cttccggcca tattcctcta
     5581 tctcgtgctg cgcttcgcaa gcgagggcat cggccacacg cgcccgatgc tctatgtggc
     5641 gcttgccgcc tttctcgtga acattccgct cgactactgg ttcatgttcg gcgggctcgg
     5701 agttccgcgg ctcggcgccg cgggatgcgg ctacgcgacg gcgctcgcgc agtggctcgc
     5761 gctcgccgtg atgctcctct acgttcgggt gtgtcgccgt cgctatgcgc cgctgcggat
     5821 ctttgcgccg atgcgccgcc cgcaattcga gggaatcgcg gcgcttctgc gcctcgggct
     5881 cccgatcggg gtgatgctct ttgccgaggt gggactcttc ggcgccgcgg cacttctcat
     5941 ggcgtccttc ggtacggtga cggcggctgc gcatcaggtg gcagttgccg tcgcctcctt
     6001 cgcgttcatg gtgccgatgg ggctcgcaac cgcgatttcc gtcgtggtcg gacaggccgc
     6061 cggcgccggt gatccgcgca acgcgcgtcg cgccggcatg gtcggcattg cgctttcgct
     6121 cggcttcgaa atcgcgtcgg cgatcctcat gttcgtcctg cgagagccgc tcgcgcggct
     6181 cttcacggcc gatccggcgg tggtcgcact cgccgccaaa ctgctcgcgc tcgccgcggc
     6241 ctttcaactt tccgacggcc tgcaggtcgc gagcgccggc gccttgcgcg gcctcaagga
     6301 cacgcgcgcg ccgatgttca tcacgctcgt cgcgtactgg ggaagcggtt tcccgcttgc
     6361 ctgggccttc gcctttccgc tggggctcgg tcccgccggc gtgtgggtgg gcctgatcgc
     6421 ggggctcacc gtcgccggcg ttcttctgtt ccttcgctac gcgcggcgat cgagacccga
     6481 tgcagccgct atgatacgag catgaatctc gacgaacgtc tgcaaggatt gctcggccag
     6541 ggtcatgacg gcgcgccgct gcgcgtatcg cttgcgctcg cctgcctcgg cagaggcgat
     6601 cccgctaatg caatcgtgca tctcgagcgt gcgctcgcgc tcgatccgga ctacagtgcg
     6661 gcatggaaaa cctacgggcg cgccctgctg acggacgccc gcgacgagga ggcgcgtgcg
     6721 gcctgggagc gcggcatcgc cgccgcgcgc gccgccggcg atcgccagtc cgaacgcgag
     6781 atgcaggtgt tcctccgccg cctcgatcca ctggatcggc aacagaagga accgacatga
     6841 acgatccatt cggactgccg ggcgcggacg tgcgcaaggc tcccgcagac gaaagtcttc
     6901 tctcgcgcga agcctggaag gtgtttcaga tcatggcgga gttcgtcgag ggatacgagc
     6961 gcctcgcgcg catccgccct tccgtttcca ttttcggttc ggcgcgggtc gcgcccgagc
     7021 atccctacta ccgcctcggc gaggaaatcg gcttcgagct ttcgcgcgcc ggattttcgg
     7081 tcgtcaccgg cggcggcggc ggtctgatgg aggccgcgaa caagggtgcc tttcgcggca
     7141 aggctccttc catcgggctc aacatccgcc tgccgaagga gcagaaggcc aacgtctggc
     7201 aggatgtcaa catcaccttc cagcactttt tctcgcgcaa ggtgatgttc gtgaagtatg
     7261 cctcggccta cgtgattctt cccggcggct tcggcacgct ggatgagttt tccgagattc
     7321 tgactttgat ccagaccggc aagagccgcc gcattccgat catcctcgtg ggcggcgaat
     7381 actggcgcgg gctgctcgat tggttccgcg atcgcctcgt gccggagcag atgatcgccg
     7441 cggaggacat tgatctcgtg tcgctcgcgg aggagccgcg tcaggtcgtg gagctgattt
     7501 tcgagcacta cggcgagcgc ggtttcgagc cttcgcccga agagcgcgaa aagatgctgg
     7561 agctgtaatt gcctgcccga tgatcgcact gctgcaacgc gtgagcgagg cttccgtttc
     7621 tgtcggcggc gaaacggtgg gcacgatcgg cgccggtctc gcggtcttcg tctgcgtgga
     7681 gcgcgacgat ggcgaggcgc aggccgcgcg tctcgtcgag cgtctcctcg gctatcgcgt
     7741 ctttgccggc gaaggcggga aactcaatcg cagtctcgcc gaaacgggag gcgggcttct
     7801 tctcgtcccg cagttcaccc tcgccgccga cacgcgcaag ggaacgcgcc cgagtctcgg
     7861 gcgggccgca ctgcccgaac aggggcgcgc gcttttcgaa cgcgtgctcg cgctcgcgcg
     7921 cgcctcgcat cccctcgtcg cgagcggcgt cttcggggca cgcatgcagg tgcggctcgt
     7981 gaacgaaggg ccggtaacct tctggctcga agcccgcgcg ggagtatcat gcgagccggg
     8041 tcgggggacg gagtaggcgg aatgggactc ggcggcatca gcgtctggca gttgatcctg
     8101 attctcgtga tcgtggtgtg cacgcttctc gcggtgccgc tcgtccatgg cggcgacgct
     8161 ccgccgtcgc cgaaaaaaga agaagacaac gacacgcccg aagaagacga gcccgaggaa
     8221 gatccgccgc tcggcgtcgg ctgagagggc ggcgccttcc gtcccgcgat caggccggct
     8281 gcaaacagga acgtggcagt tttccggaaa gctcgccggc gagcgccatt tatccgcgcg
     8341 tgccccaatc gaaggtttcg agatgatggc gcagtgccgt cgcggtcggg aagtggcgca
     8401 ggatcagact gcgtttgaga ccgaggcaga tcgtcttgac ttccgcgggc tgggcggcat
     8461 agcgcgcgcg cccgccgagc gcctggtgaa agagatacac ggcgtcgatc acgtcgtcgc
     8521 gcatgctgat gcgtcgcgat ccgctccagc gataggcatc cacgaggcgc aa
//
LOCUS       DASUJG010000098         2205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_148972, whole genome shotgun sequence.
ACCESSION   DASUJG010000098 DASUJG010000000
VERSION     DASUJG010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148972"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            312..>2205
                     /locus_tag="VFX38_01960"
     CDS             312..>2205
                     /locus_tag="VFX38_01960"
                     /inference="COORDINATES: protein motif:HMM:NF024215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEX5313661.1"
                     /translation="MKRATGLLEWWRGAPSRLVFGFAIRGTVAMMLPFVVLVELHQPY
                     GAVFAALGAQFAIFADAGGAYQRRVGAMLLALVIGAASLFVSARLPSAVWLASLVLAI
                     VAFAAGMGRVFGGSGIAIGPWASIMFLIGLLAPADTLRAAEFAGYFALGSLWVIAFQL
                     ALWRLRPYRLLFDQVAVCYEICAELVHDLAALSAGADSAAVRRRLHRRHAALREAIRT
                     AEATLEALRLGAGHSSAVFDHTLLLLAAVSREAAAAVSLRAIVWPATDTAAANIWQTL
                     FANWRQALADAAQQLSGARAEVVVAPFHAAFDTLDAQGQMPGEARAPLRLALVHLDAV
                     AESGARLSGLRFTWREALPRLSVGGLRGAWTTLAAQFTFRSMIFRHALRVAVAAGFGL
                     WISGVFDVTHRLWLPMTTILVLQPEFGATWQRLWQRVGGTLAGVLIAGGLHFLLHGTT
                     AEIVVITVFAFGMFFFIRSHYGVGVVMLTPMVLLLLGVLTAGTSASLIVARGADTLIG
                     AAIALAAAYLLWPLWQRGAFVPQCAAALRAERDYLAAAFARPGDGRISSPELMQARHR
                     AERESDNAEAMLRKMLSEPRRERANSRAALEFMTYLRRLADNALSFSIQLGGGKLTPA
                     GRAQGGE"
BASE COUNT          275 a          716 c          773 g          441 t
ORIGIN      
        1 cgtaggtaat gtaacgcata agtattgttg tcatcaaggc aatgcccagg cattggtagg
       61 ccccgcccag ggcgcgccgt ttccgggttt gccgcatccg ccggcgaccg gcggcggcta
      121 tgatgagcgc cggacgccgc cggcctggag gtactcatgc atggagccgt tcctccctgg
      181 cgctgccgtt tgtggatcgg aggagcaggg gacgcgggcg agcgcgagcc gccggccggg
      241 atgacgagcc attgctttcg tcaccttgcg tgcgcgggta cgtcttgaaa acgacgaccg
      301 aattgagcgc gatgaagcgc gcgacgggat tgctcgaatg gtggcggggt gcgccaagcc
      361 ggctggtgtt cggcttcgcg atccgcggca ccgtcgcgat gatgctgccg ttcgtcgtgc
      421 tggtcgaact tcaccagccc tacggtgcgg tgttcgcggc actcggcgcg cagttcgcca
      481 tcttcgccga tgcggggggc gcctaccagc ggcgcgtcgg cgcgatgttg ctcgcgctgg
      541 tgatcggcgc ggcgagtctg ttcgtttccg cgcgcctgcc atccgcggtc tggctcgcgt
      601 ccctggtact cgccatcgtc gccttcgcgg ccggcatggg gcgagtgttc ggcggttccg
      661 gcatcgccat cgggccgtgg gcgagcatca tgtttttgat cggcttgctg gccccggcgg
      721 ataccttgcg cgccgccgaa ttcgccggct atttcgcgct cggcagcctg tgggtgatcg
      781 ccttccagtt ggcgttgtgg cggctgcgcc cgtaccggct cctgttcgac caggtggccg
      841 tctgttacga gatctgtgcg gaactggtgc acgatttggc ggccctgtcg gccggtgcgg
      901 attccgccgc ggtgcgccgg cgtttgcacc gccgacacgc ggcgctgcgc gaggcgatcc
      961 gcacggccga ggcgacgctc gaagcgctgc gtctcggtgc ggggcattcc agtgccgtgt
     1021 tcgatcacac cttgctcctg ctcgccgccg tttcgcgcga agccgcggca gcggtcagcc
     1081 tgcgggcgat cgtctggccc gcgacggaca cagccgccgc gaacatctgg caaaccttgt
     1141 tcgcgaactg gcgacaggcg ctcgccgatg cggcgcaaca gttgtccgga gcgcgtgcgg
     1201 aagttgtggt tgcgcccttt catgccgcat tcgacacgct tgatgcgcaa gggcagatgc
     1261 cgggcgaagc gcgggcgccg ctgcgtcttg cgctcgtgca tctcgatgcc gtcgcggaaa
     1321 gcggtgcgcg cctttccggc ttgcgcttca cctggcgcga ggccttgccg cggctgagcg
     1381 tcggcggcct gcgcggagcg tggacgactc ttgccgcgca attcacattc cgctccatga
     1441 tcttccgcca tgccttgcgc gtcgccgtcg ccgcgggttt cggtttgtgg atttccggcg
     1501 tgttcgatgt gacccatcgc ctgtggttgc cgatgacgac gatcctggtg ctgcaacccg
     1561 aattcggcgc gacctggcaa cggctgtggc agcgagtggg cggcacgctc gccggggtgc
     1621 tcatcgccgg cggcctgcat tttctgctgc atggaaccac cgccgaaatc gtggtcatca
     1681 cggtttttgc cttcggtatg ttctttttca ttcgcagcca ttacggcgtc ggcgtggtga
     1741 tgctgacgcc gatggtcttg ctgctgctcg gcgtgttgac ggccgggact tccgcctcgc
     1801 tgatcgtcgc ccgcggcgcg gataccctga tcggcgcggc gatcgcgctg gcggcggctt
     1861 atctgctgtg gccgttgtgg cagcgcggcg cctttgtacc gcaatgcgcc gcggccctgc
     1921 gcgccgaacg cgattatctc gccgcggcgt tcgcacgtcc cggcgacggg cggatttcca
     1981 gcccggaact gatgcaggcg cggcatcgcg ccgaacgcga aagcgataac gccgaggcca
     2041 tgttgcggaa gatgctctcc gagccgcggc gcgaacgtgc caattctcgc gccgcgctgg
     2101 agttcatgac ctacctgcgg cgcctggcgg acaatgcgct gagcttttcg atccaactcg
     2161 gtggcggcaa gctcacgccc gccggtcgcg ctcagggcgg cgaga
//
LOCUS       DASUJG010000099         6227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_394361, whole genome shotgun sequence.
ACCESSION   DASUJG010000099 DASUJG010000000
VERSION     DASUJG010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6227
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394361"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /locus_tag="VFX38_01965"
     CDS             complement(<1..1356)
                     /locus_tag="VFX38_01965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEX5313662.1"
                     /translation="MLRKLLLASGLVLAVAPAAYPADLWQVFQLAQANDPAFREALAN
                     RNASMQAKPEAWAKIFPAIDLNASRTWDNTHSDRLSFFGTGVFPVQSSSNDRNDQWGA
                     QLTQTLFNWQQFQALKGADFTVAQAEATYQSTLQGLIVTVSQAYFNVLNARDVLNADV
                     ANAKSLGKQYQQTQEQYKVGLIAVTGVREAEAGYDQARAQVIADRQALAQAEEALRAI
                     TGQYIGQLAEPQKKLPLHPPQPESVDTWVAQGLKDNPSLIAAQFGTKVAQNQVAQQRG
                     GYMPQLNLVLQHTYQTGNGNSSFSVSGQGTTQTPDRSHDYDNQIGLQLSWNIFSGGAT
                     RAATKQAQFQADAAMAQEITQRRSIEQQVRNAYLAVLSGIAQVQATQQAVAASQISLH
                     ATEAGLSVGTRTVLDVLTARSTLLTSQKSYFNARYNYLVAVLQLEQAAGTLSPADVKQ
                     LN"
     gene            complement(1415..2083)
                     /locus_tag="VFX38_01970"
     CDS             complement(1415..2083)
                     /locus_tag="VFX38_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEX5313663.1"
                     /translation="MIVLDVNFKATRRQMVDQQIRAWGVLDPRVVAVMGSMARERFVP
                     ERYRYVAYADSDIPIGHGEVMLVPKVQGRLLQALELEEGQRVLEIGTGTGYLTACLLA
                     LGAAVSSVDIHEDFVRSAAQNLEAEGFRGADIQQEDAHTLAAAGPQYDAILVTGSLPV
                     ADPSFPRRLAVGGRLVWILGQEPAMRAEVLFRLGDDEWRSRGLFETVAPALKGTAAPR
                     RFDF"
     gene            complement(2172..2813)
                     /gene="nth"
                     /locus_tag="VFX38_01975"
     CDS             complement(2172..2813)
                     /gene="nth"
                     /locus_tag="VFX38_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX5313664.1"
                     /translation="MNSRRARAIFARFAAANPEPQSELRYRSPFQLLVAVILSAQATD
                     KGVNAATGKLFAVANTPRAIAQLGVDGLKPYVRTIGLYNGKAAHIVAMAQILLAEHGG
                     RVPRTRAALEALPGVGRKTANVVLNTAFGEPVIAVDTHVFRVAKRIGLATGDTPRAVE
                     DELQRRTPSEYRRHAHHWLILHGRYVCKARRPACWCCLIRDLCEYPDKTPAPP"
     gene            complement(2810..3463)
                     /locus_tag="VFX38_01980"
     CDS             complement(2810..3463)
                     /locus_tag="VFX38_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HEX5313665.1"
                     /translation="MQANADAVDALLPQQQCRRCGYEGCRPYAEAIAAGKARINRCPP
                     GGTLVIDALAALLDTQALALDPAVGTIEPTAVARIDAKACIGCTKCLPACPVDAIVGA
                     PKLLHDVLDAACTGCGLCLPPCPVDCITLLPGERVGDAEGRRRASPDDIEAERGRMML
                     RAEDLRRRYVQHELRLSRGRRSRRHALDDADAEDLARRRAEIAAAVARVRTARAAAR"
     gene            complement(3522..5594)
                     /gene="metG"
                     /locus_tag="VFX38_01985"
     CDS             complement(3522..5594)
                     /gene="metG"
                     /locus_tag="VFX38_01985"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011813522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX5313666.1"
                     /translation="MPRRILVTNALFYANGALHLGHMIEAIQSDIWVRFQKMRGHECH
                     YIGGDDAHGTPVMLRAREEGVTPEHLIEGMRESHRRDLADFHVAFDNYYTTHSPENRE
                     FAELFYRRLQAGRHITRRRIRQAYDEKANMFLPDRFIRGECPNCGTPDQYGDSCENCG
                     ATYAPADLKNPVSVVSGTRPVLRESEHHFFKLGDFETMLRKWTRGRDANVPDPHVDPA
                     VANKLKEWFDAGLRDWDISRDAPYFGFEIPDAPGKYFYVWLDAPIGYMASFRDYGNRH
                     PEVDFDAFWDADKAHAEGTELYHFIGKDITYFHTLFWPAMLAGAGFRSPSAVFVHGFL
                     TVNGEKMSKSRGTFITARDWLAHLSAEPLRYYFAAKLGPGVGDIDLNLDDFVARVNSD
                     LVGKYVNIASRAAGLLHGHFEGRLADPRLPLTANLFDSQRDFRMDLELMAQAYEQRAY
                     HAAMNKVTGNADRINATWQKEAPWALASSASLDTRERLHDTCSMAIQAFRLLTIALKP
                     VLPDLARKAEAFLNVAPLAWDDLGKPLPEGHRINPYEHLMTRIDPVQIEAMLEASRES
                     LAKTPPKQVLTMETNAAEIGIEDFSKLDLRVARVAKAEHVEGADKLLRLEVELGGETR
                     QVFAGIKAAYEPETLVGKLVVVVANLAPRKMRFGVSAGMVLAAGPGGKDIFLVSPDTG
                     AEPGMKVK"
     gene            5728..6147
                     /locus_tag="VFX38_01990"
     CDS             5728..6147
                     /locus_tag="VFX38_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008289938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX5313667.1"
                     /translation="MNVSQQMLEFPGRDRGTREISAEVAGVVAESGIRAGLCQVFSRH
                     TSAGVIITENADAEVRRDHERLLAHLVPDGWEEFRHRAEGDDDMSAHARTLLAGEAVL
                     VPVRNGKLGLGRWQGIFLWEHRRGAHRREIVITVIGE"
BASE COUNT         1046 a         1948 c         2090 g         1143 t
ORIGIN      
        1 gttgagctgt ttcacatccg cgggcgaaag cgttccggcc gcttgttcga gttgcaatac
       61 cgcgacgaga tagttgtagc gggcgttgaa ataagacttc tgcgaggtga ggagcgtcga
      121 gcgtgccgtc agtacgtcga gcacggtgcg ggtgccgacg ctcagccccg cctcggtcgc
      181 gtgcaaggaa atctgcgagg ccgcgacggc ctgctgcgtc gcctgcacct gggcgatgcc
      241 ggaaagtacg gcgagatagg cgttgcgcac ctgctgctcg atgctgcggc gctgcgtgat
      301 ttcctgcgcc attgcggcgt ccgcctgaaa ctgcgcctgc ttggttgcgg cccgcgtcgc
      361 gccgccggag aaaatgttcc aggagagttg caggccgatc tgattgtcat agtcgtggct
      421 gcgatcgggg gtctgagtcg tgccctgccc ggagacgctg aacgacgagt tgccgtttcc
      481 cgtctgatag gtgtgttgca gcacgagatt gagctgcggc atgtaacctc cgcgctgctg
      541 cgccacctga ttctgcgcga ccttggtgcc gaattgggcg gcgatgagcg aaggattgtc
      601 cttcagtccc tgcgcgaccc aggtatccac actttccggt tgcggcggat gcagcggcag
      661 cttcttctgc ggctccgcga gctggccgat gtattggccg gtaatcgcgc gcagcgcctc
      721 ctcggcctgc gccagcgcct ggcgatccgc aatgacctgc gcgcgggcct gatcgtaacc
      781 ggcctcggcc tcgcgcaccc cggtgaccgc gatcaagccg accttgtact gctcctgcgt
      841 ctgctggtac tgcttgccga gcgacttggc gttggcgacg tcggcgttga gcacgtcgcg
      901 cgcattgagg acgttgaaat aggcctgcga aaccgtaacg atcaggccct ggagcgtcga
      961 ctgataggtc gcctcggcct gcgcgacggt gaaatccgcc cccttcagtg cctgaaactg
     1021 ctgccagttg aagagcgtct gtgtcagttg cgcgccccat tggtcgttgc gatcgttgga
     1081 ggagctttgc accgggaata cgccggtgcc gaagaagctc agccggtcgc tgtgggtgtt
     1141 gtcccaggtg cggctcgcat tgaggtcgat cgcgggaaag atcttggccc aggcctccgg
     1201 ctttgcctgc atcgaggcat tgcggttcgc aagcgcttcg cgaaacgccg gatcgttcgc
     1261 ctgcgcgagc tggaagacct gccagaggtc ggccggatag gcggccggcg cgaccgcgag
     1321 gacaagaccc gaggcgagaa ggagctttcg gagcatggtt ttgggcaccg tgttttggtg
     1381 aactataaca ccatttcaat ggaaggacaa tcgctcagaa atcgaagcgc cgcggcgccg
     1441 ccgtcccctt gagcgcgggc gcgacggtct cgaaaagccc gcggctgcgc cactcgtcgt
     1501 cgccaaggcg aaacaggacc tcggcgcgca tggccggctc ctgcccgagg atccacacga
     1561 ggcgaccgcc gacggcaaga cgccgcggaa agctcggatc ggcaaccggc aacgagccgg
     1621 tcacgaggat ggcatcgtac tgcggacccg cggccgcaag cgtatgggcg tcctcctgct
     1681 gaatgtcggc gccccggaag ccctcggcct cgaggttctg cgccgcgctg cggacgaagt
     1741 cttcgtggat gtcgacgcta ctcaccgcgg cgccgagcgc gagcaggcag gccgtgagat
     1801 acccggtgcc ggttccgatt tccagcacgc gctggccttc ctcgagttcc agcgcctgca
     1861 acaggcgacc ctgcaccttc ggcaccagca tgacttcccc gtggccgatc gggatgtcgg
     1921 agtcggcata ggcgacatag cgatagcgct ccggcacgaa gcgctcgcgc gccatgctgc
     1981 ccatgaccgc gaccacgcgt gggtcgagca cgccccaggc gcggatttgc tgatccacca
     2041 tctggcgacg cgtcgccttg aagttcacgt cgagcacgat catcccgaag cctgcgcgat
     2101 gctgccatta tcccagatag cgcgagcgcg gccctctccg ccacgttccc gacgggctcg
     2161 ctctcctgcg gtcacggcgg cgccggagtt ttgtccgggt actcgcaaag gtcgcggatc
     2221 aggcagcacc agcatgcggg ccggcgcgcc ttgcagacat agcggccgtg gagaatcagc
     2281 cagtgatggg catgtcgccg gtactccgac ggagttctcc gctggagttc gtcttctacc
     2341 gcgcgcggcg tgtcgccggt tgcgagtccg atgcgcttcg cgacgcgaaa gacatgcgtg
     2401 tccaccgcaa tgacgggctc tccgaatgcg gtgttgagca cgacgttggc ggtcttgcgg
     2461 ccgacgccgg gcagggcttc gagcgccgcg cgcgttcgcg gtacccggcc tccgtgttcg
     2521 gcgaggagga tttgcgccat ggcgacgatg tgggccgcct tgccgttgta gagcccgatc
     2581 gtgcgaacgt acggcttgag accgtcgacc cccaattgcg cgattgcccg cggagtattg
     2641 gcaaccgcga aaagtttgcc cgttgccgcg ttgacaccct tgtccgttgc ctgggccgaa
     2701 agaatgacgg caacgagtag ctggaacggc gagcggtagc gcagctcgct ttgcggctcc
     2761 ggatttgccg ccgcgaagcg cgcaaaaatc gcgcgcgccc ggcgggagtt caccgcgccg
     2821 ccgcccgggc tgttcgcacg cgcgcgacgg cggccgcgat ttccgctcgc cgccgcgcga
     2881 ggtcctccgc atcggcgtcg tcgagggcat gcctgcggct gcgccgcccg cgggacaggc
     2941 ggagttcgtg ctgaacgtag cggcgccgca gatcctcggc ccggagcatc atgcggccgc
     3001 gctcggcttc gatgtcgtcg ggcgatgcgc gtcgccggcc ttcggcgtcg ccaacacgtt
     3061 cgcccggcag gagcgtgatg caatccaccg ggcagggcgg cagacagaga ccgcagccgg
     3121 tgcaggcggc atccagaacg tcgtgcaaaa gcttcggcgc gccgacgatc gcgtcaaccg
     3181 gacaggcggg aaggcacttc gtgcagccga tgcacgcctt tgcatcaatg cgtgcgacgg
     3241 ctgtcggttc gatcgtgccg acggcggggt cgagggcgag cgcttgcgtg tcgaggagcg
     3301 cggccagagc gtcgatcacg agcgtgccgc ccggcgggca gcggttgatc cttgctttgc
     3361 ccgccgcgat cgcctcggcg tagggccggc aaccctcata accgcaacgc cggcactgct
     3421 gctgaggcag gagcgcatcg acggcatcgg cgtttgcctg catgttcaaa gtgacgcaga
     3481 gaacgcgcgt gctatgcgtt cagaaccgct tgccggagat ctcactttac cttcatgccg
     3541 ggttcggcgc cggtatcggg agagacgaga aaaatgtcct tgccgcccgg cccggcggcg
     3601 agcaccatgc cggcggaaac gccgaagcgc atcttgcgcg gcgcgagatt ggcaactacc
     3661 acgacgagtt tcccgaccag cgtttcgggt tcgtaggcag ctttgatgcc ggcgaatact
     3721 tgccgcgtct cgccgccgag ttccacttcg aggcgcagga gtttgtccgc gccctccacg
     3781 tgttcggcct ttgccacacg ggcgacgcgc agatccagtt tcgagaagtc ttcaatgccg
     3841 atttcggcgg cgttcgtttc catcgtgagt acctgcttcg gaggagtttt ggcgagcgat
     3901 tcgcgcgagg cttcgagcat cgcttcgatt tgcacgggat cgatgcgcgt catgaggtgt
     3961 tcgtacgggt tgatgcggtg gccttcggga agaggctttc cgaggtcgtc ccaggcgagc
     4021 ggcgcgacgt tcagaaaagc ctccgccttg cgggcaagat ccggcagcac gggcttgagc
     4081 gcgatcgtga gcaggcgaaa cgcctggatt gccatgctgc aagtgtcgtg caatctttcc
     4141 ctagtatcca gactggcgga acttgcaagc gcccacggcg cttccttttg ccacgttgcg
     4201 ttaatgcggt ccgcatttcc cgtaaccttg ttcatcgccg catggtacgc gcgttgctcg
     4261 tatgcttgcg ccatgagttc caaatccatg cggaagtccc tttgcgagtc gaagagattc
     4321 gcggtcagcg gcagacgagg atcggcgagc ctgccctcaa aatgcccgtg gagcaaccct
     4381 gccgcgcggc ttgcgatgtt gacgtacttt ccgacgagat cggaattgac gcgggcgacg
     4441 aagtcgtcga gattgagatc gatgtcgccc acgccggggc cgagtttggc cgcgaagtaa
     4501 tagcgcagcg gctcggcgga tagatgtgcg agccagtcgc gggccgtgat gaaggtgccg
     4561 cgcgatttgg acatcttctc gccgttcacg gtgagaaagc cgtgtacgaa caccgcgctc
     4621 ggcgaccgaa atcccgcacc cgccaacatc gccggccaga acaaggtatg aaagtaggtg
     4681 atgtccttgc cgatgaaatg ataaagctcg gtgccttcgg catgggcctt gtctgcgtcc
     4741 cagaaggcgt cgaagtccac ctcgggatgg cggttgccat agtcgcggaa gctcgccatg
     4801 tagccgatcg gggcgtccag ccacacatag aaatatttgc ccggcgcatc gggaatctcg
     4861 aagccgaagt acggcgcgtc gcgggaaatg tcccagtccc tcagcccggc atcgaaccat
     4921 tccttcagtt tgttcgcgac cgccggatcc acgtggggat ccgggacgtt cgcatcccgc
     4981 ccgcgcgtcc acttccggag catcgtctcg aagtcaccga gcttgaagaa atggtgttcg
     5041 ctctcgcgca ggaccggtcg cgtgccggaa accaccgaaa ccggattctt caggtccgcg
     5101 ggcgcataag tggcgccgca gttttcgcag gagtcgccgt actgatccgg agttccgcag
     5161 ttcggacatt cgccgcgaat gaagcgatcg gggagaaaca tgtttgcttt ttcgtcgtag
     5221 gcctggcgaa ttctgcggcg cgtgatatgg cggccggcct gcaggcgacg atagaagagt
     5281 tcggcgaatt cgcggttctc gggggaatgc gtcgtgtagt agttgtcgaa ggccacgtga
     5341 aagtccgcga gatcgcgccg atgcgattcg cgcattcctt cgatcaagtg ttccggagtc
     5401 accccctctt cgcgcgcccg caacatgacc ggcgtgccgt gggcgtcgtc gccgccgatg
     5461 tagtggcatt cgtggccgcg catcttctgg aagcgtaccc agatgtcgct ctggatcgcc
     5521 tcgatcatgt gcccgaggtg cagcgcgcca ttggcgtaaa aaagcgcgtt ggtgacgaga
     5581 atgcggcgcg gcatcgcgaa aaactgcgca aagcaggcat tttgccacag acatgcccgg
     5641 cgcggacgct gcggcggtgc taggctacga gaacgattcc cgccgggcga acgcgatgct
     5701 ttccccgcct ttcgagacgc ttccttcgtg aacgtctcgc agcaaatgct cgagtttccc
     5761 ggccgcgatc ggggaacccg ggaaatcagc gcagaagtcg cgggcgtggt cgcggaatcc
     5821 ggcatccgcg cgggtctgtg ccaagtcttc tcccgccaca cgagcgcggg tgtcatcatc
     5881 accgaaaacg ccgacgccga ggtgcgacgg gatcacgaac gtctgctcgc gcatctcgtc
     5941 cccgatggct gggaggagtt tcgccatcgc gccgaaggcg acgacgacat gtcggcgcat
     6001 gcgcgcacgc tgctcgcggg ggaggcggtg ctggtcccgg tgcgcaacgg gaaactcggc
     6061 ctcggacgct ggcaggggat cttcctatgg gaacaccggc gcggcgcgca tcggcgcgaa
     6121 atcgtgatta ccgtcatcgg cgagtagcta cggcgccgag gtgagcggcc gcctgcgcga
     6181 ccccgggatc gcgccgcgtc agtgcgttgc ggctgccggc ggcgccg
//
LOCUS       DASUJG010000100         3726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1739541, whole genome shotgun sequence.
ACCESSION   DASUJG010000100 DASUJG010000000
VERSION     DASUJG010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1739541"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /gene="infC"
                     /locus_tag="VFX38_01995"
     CDS             complement(<1..220)
                     /gene="infC"
                     /locus_tag="VFX38_01995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX5313668.1"
                     /translation="MSIAQEKGIRRNEEITAPRVRVVGTDGQQIGVLTVEAAIQAAYG
                     AELDLVEVAPTAEPPVCRIMDYGRFVFEQ"
     gene            complement(217..2148)
                     /gene="thrS"
                     /locus_tag="VFX38_02000"
     CDS             complement(217..2148)
                     /gene="thrS"
                     /locus_tag="VFX38_02000"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX5313669.1"
                     /translation="MSAITVTLPDGSERSYSAPPRAAEIAADIGAGLARAALAARVNG
                     RLVDLDREIDADARVEIVTGRDPAGLEVLRHSTAHLLAQAVKQLYPEAQVTIGPVIEN
                     GFYYDFAFDRAFTPDDLERIEARMHEIAKTDLPVSRREVSREEAIAFFRGLDENYKAA
                     IIAAIPPEEILTLYTQGDFTDLCRGPHVPSTGRLGAFKLTHVAGAYWRGDSANEMLQR
                     IYGTAWADAKALQAYLRQREEAEKRDHRRIGKALDLFHQQEEAPGMVFWHPHGWAIWQ
                     AVEQYLRGVYRDSGYEEVRGPQILDVSLWKKSGHWDNYRENMFFTESEKREYAIKPMN
                     CPGHVQIYNRRLHSYRDLPVRYGEFGSCHRNEPSGALHGLMRVRAFTQDDGHVFCTPE
                     QIEGEVTAFHAQAMRVYADFGFRDIEVKIALRPEQRIGSDEVWDQAEEALRAALRSAA
                     VGWEELPGEGVFYGPKIEYHMRDSIGRDWQVGTMQVDFLMPERLDAEYVDETSARRRP
                     VMLHRAIVGSMERFLGILLEHYAGRLPVWLAPVQTVVLAIGEGQVDYAREVACGLARE
                     GFRVETDLRNETIGYKIREHTLGRVPYLLVVGERERAAEGVAVRTLAGKDLGTMPVGQ
                     FARQLAGEVAARGGEQTED"
     gene            complement(2196..2272)
                     /locus_tag="VFX38_02005"
     tRNA            complement(2196..2272)
                     /locus_tag="VFX38_02005"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2236..2238),aa:Val,seq:gac)
     gene            complement(2322..2915)
                     /locus_tag="VFX38_02010"
     CDS             complement(2322..2915)
                     /locus_tag="VFX38_02010"
                     /inference="COORDINATES: protein motif:HMM:NF019237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HEX5313670.1"
                     /translation="MRRAFLRISFVTAAVCILAGYGFAQTCNSSTADDAPASRYQANA
                     DGTASDLRTGLMWTRCALGQNWNAGSKSCNGKATLYDWEDALQAVQTLDQGSGYAGHA
                     DWRMPNLRELMSLSRYHCSDPAINSTIFPNTDSAAFWSSSPIEGRSGEVWAINFDAGQ
                     AFDDIMANQQELRLVRAGAFDNEIPPPASSAPAAVIH"
     gene            complement(2915..>3726)
                     /locus_tag="VFX38_02015"
     CDS             complement(2915..>3726)
                     /locus_tag="VFX38_02015"
                     /inference="COORDINATES: protein motif:HMM:NF019237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HEX5313671.1"
                     /translation="AAAQTSRAFFLQLSNPSGNLTLGTSTAAAVLRYFPEPLNDTGVT
                     GCGNATNGNPSNPDSCPQSDFPAQDAEIGRDAAANAGTLAKVGNGIFGFDFTLLGNDG
                     KPLFNQNANYAVQPWACVRDNWTGLVWEVPTPVATGGLFNTAFVYSWYDPDGQTNGGG
                     KGNANGGPRKIDTDHFVQAANDYDLCGFSDWRLPSAAEFRNLVNIGAPGTPSGILPSI
                     PTLESAGYWTATSEPVNGSTRAVTISALYGYDSFLPKTGANYVILVRGGQP"
BASE COUNT          619 a         1201 c         1218 g          688 t
ORIGIN      
        1 tctgctcgaa cacgaagcgc ccgtaatcca tgattcggca gaccggaggc tctgcggtcg
       61 gcgccacctc gaccaggtcg agttcagcgc cgtaggcggc ctggatcgcc gcctcgaccg
      121 tgaggactcc gatctgctgg ccgtccgtgc caaccacccg gactcgcggc gcggtgattt
      181 cctcgttgcg cctgatccct ttttcctgcg cgatgctcag tcctccgtct gttcgccgcc
      241 gcgcgcagcc acctcgccag ccagttgccg ggcgaactgc ccgaccggca tggtgccgag
      301 atccttcccg gccagtgtgc gcacggccac accttccgcg gcccgctcgc gctcgccgac
      361 gacgagcagg tacggaacgc gacccagtgt atgttcgcga attttatagc ctatcgtctc
      421 gtttctcaag tcggtttcca cccgaaaacc ctcgcgagcg aggccgcagg cgacctcgcg
      481 ggcataatcg acctggccct cgccgatcgc gagcaccacc gtctggaccg gtgcgagcca
      541 caccggaagg cgtccggcgt aatgctcgag gaggatgccg aggaagcgct ccatcgagcc
      601 cacgatcgcc cggtgaagca tcacggggcg gcggcgggcg ctggtctcat ccacgtattc
      661 ggcatcgagg cgctcgggca taaggaaatc cacctgcatg gtgcctacct gccaatcgcg
      721 gccgatggag tcccgcatgt gatattcgat cttgggccca tagaagactc cctcgccggg
      781 aagctcctcc cagccgacgg cggccgagcg tagcgccgcg cgcaatgcct cctcggcctg
      841 atcccagact tcatccgagc cgatgcgctg ctcgggccgc agcgcgatct tcacctcgat
      901 gtcgcgaaag ccgaagtcgg cgtagacgcg catcgcctgc gcgtggaacg cggtcacttc
      961 gccctcgatc tgctccggcg tgcagaagac atgcccgtcg tcctgggtga aggcgcgcac
     1021 gcgcatcaaa ccgtggagcg cacccgaggg ctcgttgcga tgacagcttc cgaactcgcc
     1081 gtaacggacc ggcagatcgc gataactatg gagacgccgg ttgtagatct gcacgtggcc
     1141 ggggcaattc atcggcttga ttgcgtattc gcgcttctcg gattcggtga agaacatgtt
     1201 ttcgcggtaa ttgtcccagt gcccggactt cttccacagc gagacgtcga gaatctgcgg
     1261 gccgcgcact tcttcgtagc cggagtcgcg gtacacgccc cgcagatact gttcgaccgc
     1321 ctgccagatc gcccatccgt gcggatgcca gaagaccatg cccggcgctt cttcctgctg
     1381 gtgaaagaga tcgagcgcct tgccgatgcg gcggtggtcg cgcttctccg cctcttcgcg
     1441 ctggcgcagg taggcctgga gcgccttcgc atccgcccag gcggtgccgt agatgcgctg
     1501 cagcatttcg ttcgcggaat cgccgcgcca gtaggcacct gcgacgtggg tgagtttgaa
     1561 ggcaccgagt ctgccggtcg aaggcacgtg gggaccgcgg cagagatcgg tgaaatcgcc
     1621 ctgcgtgtag agcgtcagga tttcttcggg cggaatcgcg gcgatgattg ccgccttgta
     1681 gttttcgtcg agtccgcgaa agaaggcgat ggcttcctcg cgcgagacct cgcggcggga
     1741 aacgggaaga tccgtcttcg cgatttcgtg catgcgcgcc tcgatgcgtt cgagatcgtc
     1801 gggcgtgaac gcgcgatcga aggcaaaatc gtaatagaag ccgttttcga ttaccgggcc
     1861 gatggtgacc tgggcctcgg gatagagctg cttgacggcc tgggcgagca gatgcgcggt
     1921 ggagtggcgc agcacttcga gtccggccgg atcgcggccg gtgacgattt cgacgcgcgc
     1981 gtcagcgtcg atctcgcgat cgagatccac cagacgaccg ttcacgcggg cggcaagcgc
     2041 cgcacgggcg agtcccgcgc cgatgtcggc ggcgatctcg gccgcgcgcg gcggagcgga
     2101 gtaactccgc tcgctgccgt ccggcagggt tacggtgatt gcgctcatcg cggccggccc
     2161 cacaaaagaa aaacaccgcc cggcggtgct ttcaatggta ggcgcgattg gactcgaacc
     2221 aacgaccccc accatgtcaa ggtggtgctc taaccagctg agctacgcgc cttcgaatcg
     2281 gcgggcgaca agccgtggcg cttcgcgccg cccgtcagcg gctaatgtat cacagcggcc
     2341 ggcgccgagg aggccggcgg cggtatttca ttgtcgaagg cgcccgcgcg cacgagccgc
     2401 aattcctgct ggtttgccat gatgtcgtcg aaggcctgac cggcatcgaa attgatcgcc
     2461 cacacctccc cgcttcttcc ctcgatcggc gaggaactcc agaaggccgc cgagtcggta
     2521 ttcgggaaaa tcgtcgagtt gatcgccgga tcgctgcagt ggtagcggct cagggacatg
     2581 agttcgcgca ggttgggcat gcgccagtcc gcgtgccccg cgtagcccga accctgatcg
     2641 agtgtctgca ccgcctgcag cgcatcctcc cagtcgtaga gcgtggcctt tccgttgcag
     2701 cttttgctcc ccgcgttcca gttctggccg agtgcgcagc gtgtccacat gaggccggta
     2761 cggagatcgc ttgccgtgcc gtctgcgttc gcctgatagc ggctcgctgg cgcgtcgtcc
     2821 gctgtggagg aattgcaggt ttgcgcgaag ccgtagccgg cgaggatgca gacggcggcg
     2881 gtcacgaagc tgattcgcaa aaatgcgcgg cgcatcacgg ttgtcctccc cgcaccagaa
     2941 tgacgtagtt cgcgcccgtc ttcggcagaa agctgtcgta gccgtagagt gcgctgatgg
     3001 tcaccgcccg ggtgctgccg ttcacgggtt cggaggtcgc ggtccagtaa ccggcgcttt
     3061 cgagcgtcgg aatcgagggc aaaattccgc ttggcgtgcc cggcgcgccg atgttgacca
     3121 gattgcggaa ctcggccgca ctcggcaggc gccagtcgct gaagccgcag aggtcgtagt
     3181 cgttggccgc ctgcacgaaa tgatcggtat cgatcttgcg cggcccgccg ttcgcattgc
     3241 ccttgccgcc gccgttcgtc tgcccgtccg gatcgtacca actgtagacg aaggcggtgt
     3301 tgaaaagccc cccggtcgca accggcgtcg gcacttccca gacgaggccg gtccagttgt
     3361 cgcgcacgca ggcccagggc tggaccgcgt aattcgcatt ctggttgaac agcggcttgc
     3421 cgtcgttgcc gagcaaagtg aagtcgaatc cgaaaatgcc gttgcccacc ttggcgagag
     3481 ttccggcatt ggcggcggcg tcccgcccga tctcggcgtc ctgcgccggg aaatcgctct
     3541 gcgggcagga atccggattg gacggattgc cgttggttgc gttgccgcaa ccggtgacgc
     3601 cggtatcgtt gagcggctcg gggaaatagc gcagcacggc agcggccgtg ctcgttccga
     3661 gggtcagatt gccgctcgga ttgctgagct ggagaaagaa ggcgcgcgaa gtctgcgctg
     3721 ccgcac
//
LOCUS       DASUJG010000101         4183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_638254, whole genome shotgun sequence.
ACCESSION   DASUJG010000101 DASUJG010000000
VERSION     DASUJG010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638254"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..3564
                     /gene="smc"
                     /locus_tag="VFX38_02020"
     CDS             70..3564
                     /gene="smc"
                     /locus_tag="VFX38_02020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEX5313672.1"
                     /translation="MRLKRIRLAGFKSFVDPTDVELPSALVGVAGPNGSGKSNIVDGV
                     RWVMGESSARHLRGEAMADVIFNGSTGRQPLGSATVEMVFDNSEGRLGGEYANWSEIA
                     IRRTVDREGISSYYLNGTRCRRRDITDVFLGTGLGPRSYAIIEQGTISRLIESKPEEL
                     REFLEEAAGISRYRERRRETENRIHHTRENLARVDDVLEEVGKQLSHLRRQARAAERY
                     RDLKAEERQRRSELLALRIAALDVELTTREDEVRGQSVGLEAELARERATERDLVVAR
                     AGHDAAVDEAGTVQQRYFELQTEIARLEEGIAAARREHALHQDRAGALEARLRANAER
                     REAQEKQAAALRRELARREPELGAAEDSARAAQASSADAEAAAEGLRGEDAAGREAFA
                     ETRRRIEVLEARLAGCADREEHLQRRGERIADERSRRQELDEDRSAEGARVTLEALKQ
                     RANVAQDEVADAARQVAQWRQRFDAAGETFANLGRDAAGVEGRLESLEQLVRARRGGE
                     AAEEWLRAHDLERARRLVSSLDIAPGWEWALEVALGARLAAVEIDALDVDSLTDLARL
                     AGGDLSFVESGAGTAAAGTLAARIAAPSPALLELLAGVREASSAAEAFAERDRLAPGE
                     SFLTRDGTWIGRHWLRVARPGEGESGAIGLERERLQLLGRRDALVGELAAAEESRMDA
                     ERRLQTALGAEAAAREASREAQEALAAGRAAEAELAGRERASRERLAALGAEATEVEN
                     ALGDSEQEHRSLDTAMREARDALARIEAARRGEEPMLAEALTLARAKRMELEAAERAR
                     EGLHLQVELARAQSTSAEEAAERLRGESETLAAERAALLGRISETSEPGGELRKDLEE
                     RLEARHGIDAELASKRDRAAGIEMRVQELERERRAVAARVGELRETLERARLASQELR
                     TRREGLAEQRAELGSELAAVLDAIEPGAEPGEWERRIEDLAKRIQRLGAINLAAIDEC
                     AAQSEREEYLQRQRADLNEALATLEDAMRRIDRETRARFRETFDRVNARFNELFPRLF
                     GGGSAALVLTGEELLDAGVVVTARPPGKKNTGIQLLSGGEKALVAVALVFAIFELNPA
                     PFCLLDEVDAPMDDSNVERFCALVREMSQRTQFVLITHNRRSMALCHQLIGVTMQEPG
                     VSRLVGVDIDEAERLAVG"
     gene            3577..>4183
                     /locus_tag="VFX38_02025"
     CDS             3577..>4183
                     /locus_tag="VFX38_02025"
                     /inference="COORDINATES: protein motif:HMM:NF016257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HEX5313673.1"
                     /translation="MWVVYLILIVLGLALIAGVYFYTRSHPQDAARGERVVSNFPDAG
                     VADVPPRPRPRARIREEPSRASSRTQPAASESRILSLHLRLPDSGLPAATVLDLLREL
                     GFVPGEHQIFHRFDEDGSLLYSVADLFEPGVLHPLGEEARLRGLSFFFESRTGAAAIA
                     RLDRMLGAVYECAERLGGHIEDREHHPLTAARELELKLTAAG"
BASE COUNT          691 a         1368 c         1535 g          589 t
ORIGIN      
        1 ggcatggcga caccgtcgtg atttcgggcg ataatttaca ctaacggccg gcgggaatat
       61 tggctccgga tgcggctcaa gcggatcagg ctggcgggat tcaaatcttt cgtggatccc
      121 accgacgtcg aactgcccag cgcgctcgtc ggcgtggccg ggcccaacgg ctcgggcaag
      181 tcgaatatcg tcgacggcgt gcgctgggtg atgggggaga gctccgcgcg ccacctgcgt
      241 ggcgaggcga tggccgacgt catcttcaac ggctcgaccg gccgccagcc gctcgggagc
      301 gccaccgtcg agatggtctt cgacaactcc gagggccgac tcggtggcga atacgcgaac
      361 tggagcgaaa tcgccattcg ccgcacggtg gatcgcgaag gaatttcgag ctattacctg
      421 aacggcacgc gctgccggcg gcgcgacatc accgatgtgt tcctcggcac cggtctgggg
      481 ccgcgcagct acgccatcat cgagcagggc acgatcagcc gcctgatcga atccaagccc
      541 gaggaacttc gcgagttcct cgaagaggcc gccggcatat cgcgctaccg cgagcggcga
      601 cgggaaaccg agaaccgcat tcatcacacc cgcgagaatc tcgcgcgcgt ggacgacgtg
      661 ctcgaggagg tgggcaaaca gctttcccat ctgcggcgcc aggcccgtgc cgccgagcgc
      721 tatcgcgatc tcaaggccga ggagcgtcag cgccgctcgg agttgctcgc gctgcgcatc
      781 gctgccctcg atgtcgaact cacgactcgc gaagacgaag tgcgcggcca gagtgtcggc
      841 ctcgaggccg agctggcgcg agagcgggcc acggaacgcg atctcgtcgt cgcgcgggcc
      901 gggcacgacg ccgccgtcga cgaggccgga accgtacagc agcggtactt cgaacttcag
      961 accgagatcg cgcggctgga agaagggatt gccgcggcac gccgcgaaca cgcgctccat
     1021 caggaccgcg cgggcgcgct cgaggcgcgg cttcgcgcca atgccgaacg ccgcgaggct
     1081 caggagaagc aggccgcggc gctgcgccgg gagcttgctc ggcgcgaacc ggaactcggc
     1141 gccgccgagg acagcgcgcg cgctgcgcag gcgtcatccg cggacgccga ggccgcggca
     1201 gaggggctgc gcggcgagga cgcagcgggc cgcgaagcgt tcgccgagac ccggcgccgg
     1261 atcgaagtgc tcgaagcgcg ccttgcgggc tgcgcggacc gggaagagca tctgcagcgt
     1321 aggggcgagc gcatcgccga cgagcgatct cgccggcagg aactcgacga ggatcggtcg
     1381 gccgagggcg cgcgcgtcac gctggaagca ctgaagcagc gggcgaacgt cgcgcaagac
     1441 gaggtcgcgg acgccgcgag gcaggtcgcg cagtggcggc agcgcttcga tgccgccggc
     1501 gaaacgttcg cgaatctcgg gcgggatgcg gccggggtcg agggaaggct cgagtctctc
     1561 gagcaattgg tgcgtgcgcg gcgcggcggg gaagccgcgg aggagtggct gcgcgcgcac
     1621 gatctggaac gggcgcggcg gctcgtgagc agcctcgaca tcgctcccgg ctgggaatgg
     1681 gcgctcgaag tcgcgttggg cgcgcgcctc gcggcggtcg agatcgacgc attggacgtc
     1741 gacagtctca ccgacctcgc gcggctcgcg ggcggagatc tgagtttcgt ggagtcgggg
     1801 gccggcacgg cggcggcggg tacgctcgcg gcgcggattg ccgcaccctc gccggcgctg
     1861 ctcgagcttc tcgcgggcgt gcgcgaggct tcgagcgccg ccgaagcgtt cgccgagcgc
     1921 gatcgcctcg ccccgggcga atccttcctg acccgcgacg gaacctggat cgggcgccac
     1981 tggctgcgcg tggcgcgacc cggcgaaggt gaaagcggcg ccatcggtct cgaacgggaa
     2041 cggctgcaac ttctgggccg gcgcgatgcg ctagtcgggg aactggccgc ggccgaggag
     2101 tcccgcatgg atgcggagcg gcggctgcaa acggctcttg gcgcggaggc ggccgcgcgc
     2161 gaagcgagtc gcgaggcgca ggaggcgctg gcggcaggac gcgccgccga agccgagctt
     2221 gccgggcgcg aacgggcaag ccgcgaacga ttggcggcgc tcggcgcgga agcaaccgag
     2281 gtcgagaatg cgctcggcga ctcggaacag gagcaccgga gtctcgacac ggccatgcgc
     2341 gaagcgcgcg acgcgctcgc acgcatcgag gccgcgcgcc gcggtgagga accgatgctc
     2401 gccgaggcgc tcacgcttgc acgtgcgaag cgcatggaac tcgaggctgc ggagcgcgcg
     2461 cgcgagggcc tgcatcttca ggtcgaactt gcccgggcgc aatcgacatc tgccgaagag
     2521 gcggcggagc ggctgcgcgg cgaaagcgaa acgctggcgg ccgagcgcgc cgcgctgctc
     2581 gggcggatca gcgaaacgag cgaacccgga ggcgagctgc gcaaggatct cgaggagcga
     2641 ctcgaggcac gtcacggaat cgatgcggaa ctcgcatcga agcgcgatcg cgccgccggg
     2701 atcgaaatgc gggtgcagga actcgagcgg gagcggcgcg cggtcgccgc acgcgtcgga
     2761 gaattgcgcg aaaccctgga gcgggcgcga ctcgcgagtc aggagttgcg cacgcggcgc
     2821 gaaggcctcg cggaacaaag ggcggagctc ggttcggaac tcgccgcggt gctcgatgcc
     2881 atcgagcccg gtgctgagcc cggcgagtgg gagcggcgga tcgaggatct cgcgaagcgc
     2941 attcagcgcc tcggagcgat caacctcgcg gcgatcgacg aatgcgcggc gcagtccgag
     3001 cgcgaagaat atctccagcg ccagcgcgcg gacctcaacg aggcgctggc gactctggaa
     3061 gacgcgatgc ggcgcatcga tcgcgagacc cgcgcacgct tccgggaaac gttcgaccgc
     3121 gtcaacgccc gcttcaacga actcttcccg cgcctgttcg gcggcggttc ggcggcgctg
     3181 gttctgaccg gcgaggagtt gctcgacgcc ggcgtggtgg tgacggcgcg cccgcccggc
     3241 aagaaaaaca ccggcatcca gttgctttcg ggcggggaga aggcgctcgt cgcggtcgcc
     3301 ctcgttttcg cgattttcga actcaacccg gcgcccttct gtctgcttga cgaggtggac
     3361 gcaccgatgg acgattccaa cgtcgaacgt ttctgcgcgc tcgtgcggga gatgtcacag
     3421 cgcacgcaat tcgtgctcat cacgcataac cgccgcagca tggcgctgtg tcaccagttg
     3481 atcggcgtga ccatgcagga gccgggcgtt tcgcggctgg tcggtgtgga catcgacgag
     3541 gccgaacgcc tcgccgtggg ctgagcgtca gcaccgatgt gggtcgtata tctgattctg
     3601 attgtcctcg gcctcgcgct cattgccggg gtgtatttct acacgcgctc gcacccgcag
     3661 gacgcggcgc gcggtgaacg agtagtctcg aacttccccg atgcgggggt cgcggatgtg
     3721 cctccgcggc cgcggcctcg cgcccggatc cgcgaggaac cgtcgcgagc ctcgtcccgc
     3781 acgcagccgg cggcaagcga gtcgcggatt ctgagtctcc atctgcgcct gccggattcc
     3841 ggtctcccgg cggccacagt gctcgacctg ctccgcgagc tcggcttcgt ccccggcgaa
     3901 catcagattt ttcatcgttt cgacgaggat ggatcgcttc tctacagcgt ggcggatctg
     3961 ttcgagcccg gtgtccttca tccgctcggc gaggaggcac gcctgcgcgg gctctccttt
     4021 ttcttcgaat cgcgcaccgg cgccgccgcg atcgcgcgcc tcgatcgcat gctcggagcg
     4081 gtctacgaat gcgccgagcg cctcgggggc cacatcgagg atcgcgaaca tcatccgctc
     4141 accgccgccc gcgaactcga actcaagttg acggcggccg gcg
//
LOCUS       DASUJG010000102         3640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1617542, whole genome shotgun sequence.
ACCESSION   DASUJG010000102 DASUJG010000000
VERSION     DASUJG010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1617542"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1404
                     /locus_tag="VFX38_02030"
     CDS             <1..1404
                     /locus_tag="VFX38_02030"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEX5313674.1"
                     /translation="YLFPDSSGLLHLEYLPHDRERGKPRGLFQYRDHTRLFSHWNLST
                     SLEFLSDDDYLRDFGTDLAQVSQTYQYRRVTASYQAAAGSAFITLQDLAPLDPSITAA
                     ERPYRKLPEIGFDFSWPNYTTGLEFSLFNDFTRFEAPERQGALREDLRPAIAEHFGNA
                     SWYATPRLAYDLTRYDLHDFGNAPAGTVNRDTPIFSFDSGLVFDRTLGKGGWLTQTLE
                     PRAFYLYVPYRDQSEIPIFDTYQPPLTMEQLFSTNRFVGLDRLGDANQLSLGLSSLFI
                     DSASGEQLLSLGIGQAFYFRDRDVTLPGEAPETSARSDYVGEVAATLSPKFSASLTVD
                     YNPYDHEFDQGYLSFQYHPRTYQVLNVGYLYRQGQLDQTDLSFAWPIAGNWSGFGRWN
                     YSILDHQTVETMAGLQYDSCCWRLRIAERRYATINGQGSYALYLELQLKGLGSIGNRV
                     SDLLQHDIYGYGENPDY"
     gene            1376..2791
                     /locus_tag="VFX38_02035"
     CDS             1376..2791
                     /locus_tag="VFX38_02035"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF020872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5313675.1"
                     /translation="MGMAKIRIIRRTAPLALAALWALPALAAPAPPAASAPPAASAPL
                     SGPGALLDEVVAVVNDQPILESALDEELARVTSALQAQGTPLPPENVLRHQVLERLIT
                     QSLELQAADHQGIQVSDDQVNQALSAIAQRNNVTLAELPQALAQQGQNYSAFRSMVRD
                     QLIVHQLIQQVVAANIAISPTEIDQYRLNQSRQQAGSIEYQLAQILVAFPPNVTPDQA
                     KATLAKAQAIETKLEGGADFAATAVAESNGPHALEGGTLGWFKGADLPTFFVDVVPAM
                     KAGEISSPIAGIGGYHIVKLLATRKPDEKSVKTEYDVEHILIRPNPVRDMSQAQALAE
                     KLRQQILAGKTGFAAAAKQYSGDPNSAGNGGSLGWVTTADLPPAIGTALAGLAVNALS
                     EPISTRYGWDLVRVAGTRQSDTSQSAQENAAYEAIFQRKLADQIAEFTRTLRGQAYIH
                     IINPADAGTSGSPAAVTPGTS"
     gene            2815..>3640
                     /gene="pdxA"
                     /locus_tag="VFX38_02040"
     CDS             2815..>3640
                     /gene="pdxA"
                     /locus_tag="VFX38_02040"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX5313676.1"
                     /translation="MRLALSVGEPAGIGPDLAVLAAQRVYPFEIVAFASPSLLASRAT
                     ALGLALAIERYDSARVPAPAKAGHLVVAPQPLVNPAIAGRLDRANAAPVLGALDAAFA
                     ACSRGECDALVTAPLHKGIINDAGIAFRGHTEYLAELAGGATPVMLLVAGELRVALAT
                     THLPLAEVPRAITAARLRAVIEALSAGLARDFGIAEPRILVLGLNPHAGEGGHLGGEE
                     QAVIAPTLAALRGRGYRLAGPVPADTAFVPARLAQTDAVLAMYHDQGLPVLKYAGFG"
BASE COUNT          612 a         1294 c         1098 g          636 t
ORIGIN      
        1 tatctctttc ccgacagttc cggactgctt catctggaat acctgccgca tgatcgcgaa
       61 cgtggcaagc cccgggggct cttccagtat cgcgaccata cccggctttt ttcgcattgg
      121 aatctgtcga cttcgctgga atttctctcc gacgacgact atctgcgcga cttcggaacc
      181 gatctcgccc aggtctcgca gacctaccag taccgccggg tcaccgccag ctatcaggcc
      241 gcggccggct ccgccttcat cactctgcag gatctcgcgc ccctcgatcc ttcgatcacg
      301 gcggccgaac gcccgtatcg caagctcccc gagatcggtt tcgacttttc ctggccgaat
      361 tacacgaccg gcctcgagtt ttccctgttc aacgatttca cccgattcga ggctcccgaa
      421 cgtcagggcg ccttgcgcga ggatctccgg ccggccatcg ccgagcattt cggcaacgcg
      481 agctggtacg caacgccgcg tctcgcctac gacctcaccc gctacgatct gcacgatttc
      541 ggcaacgccc ccgcgggcac ggtcaatcgc gacacgccga ttttctcctt cgacagcggc
      601 ctcgtcttcg atcgcacgct cgggaaaggc ggctggctca cgcagaccct cgagccgcgc
      661 gcgttttatc tctacgttcc ctatcgcgat cagagcgaaa ttccgatctt cgacacctac
      721 cagccgccgc tcaccatgga gcaactgttc tcgacgaatc gcttcgtcgg cctcgaccgg
      781 ctcggcgacg ccaatcaact ctcgctcggt ctgagcagcc tgttcatcga cagcgcgagc
      841 ggcgagcaac tgctttcgct cggtatcggc caggcgtttt acttccgcga ccgcgatgtg
      901 actctccccg gcgaggcccc ggaaaccagt gcgcgctcgg attacgtcgg cgaagtcgcg
      961 gccaccctca gcccgaaatt ttccgccagc cttaccgtcg actacaatcc ttacgaccac
     1021 gaattcgatc agggctacct ctccttccag tatcatcccc ggacctatca ggttctcaac
     1081 gtcggctatc tttaccgtca gggccagctc gaccagacgg atctgtcctt cgcctggccg
     1141 atcgcgggga actggagcgg cttcgggcgc tggaactatt cgatcctgga ccatcaaacg
     1201 gtggagacga tggccggcct acaatatgac tcctgttgct ggcgcctgcg catcgccgag
     1261 cggcgctatg cgaccatcaa cggccagggc agctatgcgc tctatctgga actgcaactc
     1321 aaggggctcg gcagcatcgg caaccgcgtg agcgatcttc tccaacatga catctatggg
     1381 tatggcgaaa atccggatta ttaggcgaac ggcacccctc gcgctcgcgg ccctgtgggc
     1441 gctgccggcg ctcgcggcgc cggccccgcc ggcggcgtcg gcgccgcccg cggcgagtgc
     1501 gcccctgagc ggacccggcg cgctgctcga cgaggtggtc gccgtcgtga acgatcagcc
     1561 gatcctcgag agcgccctcg acgaggaact cgcgcgcgtg acttcggcgc tccaggcgca
     1621 gggcacgccg cttcctcccg agaacgtgct tcgtcatcag gtgctcgagc ggctgatcac
     1681 gcagagcctg gaactgcagg ccgccgatca tcagggcatc caggtgagcg acgaccaggt
     1741 gaaccaggcg ctctccgcca ttgcccagcg caacaacgtg accctggccg agctcccgca
     1801 ggcactcgcc cagcagggac agaattattc cgcgtttcgc agcatggtgc gcgatcagtt
     1861 gatcgttcat cagttgattc agcaggtcgt cgccgccaac atcgcgattt ctccgaccga
     1921 aatcgatcaa taccgtctca accagagtcg ccagcaggcg ggcagcatcg agtatcagct
     1981 cgcgcagatc ctcgtcgcgt ttcctccgaa cgtgacgccg gatcaggcga aggcgaccct
     2041 cgccaaggcg caggccatcg agacgaaact cgagggcgga gccgattttg cggccaccgc
     2101 ggtcgccgaa tccaacggcc cgcatgcgct cgaaggcggc accctcggct ggttcaaggg
     2161 ggccgatctt ccgacctttt tcgtcgatgt ggtgccggcc atgaaggcgg gcgaaatttc
     2221 atcaccgatc gcgggaatcg gcggctatca catcgtgaag ctgctcgcga cacgcaagcc
     2281 cgacgagaag agcgtgaaga ccgagtatga cgtcgagcac attctcatcc gccccaatcc
     2341 cgtgcgcgac atgagccagg cgcaggcgct cgccgaaaaa ctgcgccagc agattctcgc
     2401 cggcaagacc ggtttcgccg cggccgccaa acagtattcg ggcgacccca attcggcggg
     2461 taacggcggc tcgctcggct gggttacgac ggcggatctt ccgccggcga tcggcacggc
     2521 gctcgcgggc ctcgcggtaa acgccctgag cgagccgatc agcacgcgct acggctggga
     2581 tctcgtgcgc gtggccggca cgcgccagag tgatacctcg caaagcgcgc aggagaacgc
     2641 ggcctatgag gcgatcttcc agcgcaagct cgccgatcag atcgccgaat tcacgcgcac
     2701 gctccgcggc caggcctaca tccacatcat caatccggcc gacgccggca cctcgggcag
     2761 ccccgccgcg gtgacgcccg ggacttcctg aggcggaacc gcagtctcgt gagcatgcgg
     2821 ctcgcgttgt ccgtgggcga gcccgccgga atcggtcccg atctcgcggt gctggccgca
     2881 caacgcgtct accccttcga aatcgtcgcc ttcgcgtccc cctcgctgct tgcctcgcgg
     2941 gcgacggcgc tcgggctcgc gctcgcgatc gaacgctacg attcggcgcg cgtcccggct
     3001 cctgccaagg cgggccacct cgtcgtcgcg ccgcagccgc tggtaaatcc cgcgatcgcg
     3061 ggtcggctcg atcgggccaa cgcggcgccg gtgctgggcg cgctcgatgc ggcattcgcc
     3121 gcctgctcgc gcggcgaatg cgatgcgctc gtcaccgcgc cgctccacaa gggaatcatc
     3181 aacgatgccg gcatcgcctt tcgcgggcat acggaatatc tggcggaact cgccgggggc
     3241 gcaacgccgg tgatgcttct ggtcgccggg gaactgcggg tggcgctcgc gacgacgcat
     3301 ctcccgctcg ccgaagtgcc gcgggcgatt accgccgcgc gtctgcgtgc cgtcatcgaa
     3361 gcgctctcgg ccggactcgc gcgtgatttc ggcatcgccg agccgcgcat tctcgtcctc
     3421 ggactcaacc cgcatgccgg cgaggggggg catctcggcg gcgaggagca agcggtcatc
     3481 gccccgacgc tcgccgcgct acgcgggcgc ggctaccggc tcgcggggcc ggttccggcc
     3541 gacaccgcct tcgtgccggc acggctcgcg caaaccgacg cggtgctcgc catgtatcac
     3601 gatcagggcc ttccggtgct gaaatacgcc ggcttcggcc
//
LOCUS       DASUJG010000103         3010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1740981, whole genome shotgun sequence.
ACCESSION   DASUJG010000103 DASUJG010000000
VERSION     DASUJG010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1740981"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VFX38_02045"
     CDS             <1..362
                     /locus_tag="VFX38_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX5313677.1"
                     /translation="SGGEAQRVKLARELARRDTGRVLYVLDEPTTGLHFHDIRQLLAV
                     LLRLREHGNSIVVIEHNLEVIKTADWIIDLGPEGGAGGGRLVASGSPEAVAANPASHT
                     GAFLARALAQAAHGTAR"
     gene            359..2128
                     /locus_tag="VFX38_02050"
     CDS             359..2128
                     /locus_tag="VFX38_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX5313678.1"
                     /translation="MSWEAIFTLAVLGATVIALAWPRMPPDLPLVGAMALLAVTGAAP
                     LKEVFEGFANPGLISVAALYVVAAGLQHTGAVTGPARWIFGRSRKLWVSQLRVMVPTA
                     AISAFMNNTPVVAALMPAVLDWAGRRRIAASRLLMPLSFATILGGTCTLIGTSTNVIV
                     NGLLVATPGEHGMGFFTIGIVGLPVAIVGIAYMVIFGRRLLPDRRSALGELTDPREYT
                     VEMLVSQTSSFSGQTLEAAGLRHLPGLYVVEIERSGRLISAPGPEEMLESGDRLVFAG
                     IVESVADLQKMRGLVPATGQVFKLDTPRPDRRLIEAVVAPENPVVGRTVREGGFRSRY
                     GAVVIAVARRGQRIKRKIGEIALEPGDTLLLEAPAEFLRRYRHHRDFLLLRPLEGPVQ
                     PHYERAWIAWAILGAMIGCVTAGLVPLAPAAVFAAVLMVATRCIGLNAARRAIELQVI
                     LIIGASFAIGAALVHTGAAEAIARPMLALAQDSPLGLLAMIYAITTILTSFISHNAAA
                     VLMFPLMLAAVGALGQPVLPFAIAIAMAASASFATPISYQTNLMVYGPGGYQFLDFVR
                     FGLPLNVVVGAVSIFVIHQVWLA"
     gene            2331..2861
                     /locus_tag="VFX38_02055"
     CDS             2331..2861
                     /locus_tag="VFX38_02055"
                     /inference="COORDINATES: protein motif:HMM:NF024586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5313679.1"
                     /translation="MQIGDDRYRSLFATIDEGLCVLEVEYGGDGNIADLRFREVNEAF
                     ERQSGMREVLGKPLSRFLPDFARQRAGAIARVLETGGPLRLENFHADTGRWYRVQYSR
                     IGGERSRLLAAVFEDVTERKFAERTANRLAAIVDSSDDAIISKDLDGSSRPGTRARSA
                     SSATARKRPSGARSRC"
     gene            2810..>3010
                     /locus_tag="VFX38_02060"
     CDS             2810..>3010
                     /locus_tag="VFX38_02060"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5313680.1"
                     /translation="MFGYRAEEAIGRPVTMLMPPDRVDEEPGILARIRRGERIDHYET
                     VRQRKDGTLLDISLSVSPIFDRE"
BASE COUNT          439 a         1058 c          986 g          527 t
ORIGIN      
        1 tctcgggcgg cgaggcgcag cgcgtgaaac tcgcccgcga actcgcccgg cgcgacaccg
       61 gtcgtgtact ctacgtgctc gacgagccga ccacggggct gcactttcac gacatccgcc
      121 agctcctcgc ggtgctgttg cgcctgcgcg agcatggcaa ctcgatcgtc gtgatcgagc
      181 acaatctcga agtgatcaag accgcggact ggatcatcga tctcggcccc gaaggcggcg
      241 cgggaggcgg acggctcgtc gcctcgggtt cgccggaagc cgtcgccgcg aatcccgcct
      301 cgcataccgg agcctttctc gcccgcgcac tcgcgcaggc ggcgcacggg accgcgcgat
      361 gagctgggag gcgatcttca ccctcgccgt gctcggcgcg accgtgatcg cgctcgcctg
      421 gccgcggatg ccgccggatc ttccgctggt cggcgcaatg gcgctgctcg cggtaaccgg
      481 ggccgcgccg ctcaaggaag tcttcgaggg attcgccaac ccggggctca tttcggtcgc
      541 ggcgctctac gtcgtcgccg ccggtctgca gcacaccggc gcggtcaccg ggccggcgcg
      601 ctggatcttc ggacgatcgc gaaaattgtg ggtctcgcag cttcgcgtga tggtgccgac
      661 ggcggcgatc agcgccttca tgaacaacac gccggtggtc gcggcgctga tgccggcggt
      721 gctcgactgg gcggggcggc ggcgcatcgc ggcctcgcgt cttctcatgc cgctgtcttt
      781 cgcgaccatt ctcgggggca cctgcacgct catcggcacc agcaccaatg tgatcgtgaa
      841 cggacttctc gtcgccactc ccggcgagca tggcatgggg ttcttcacga tcggcatcgt
      901 cggacttccg gtcgcgatcg tcggcatcgc ctacatggtg atcttcgggc gcagacttct
      961 tcccgaccgc cgaagcgcgc tcggcgaact caccgatccg cgcgaataca cggtggaaat
     1021 gctggtctcg cagacgagtt ccttttcggg acagacgctg gaggccgcgg ggctgcgtca
     1081 tctgccggga ctctacgtcg tggaaatcga aaggagcggg cggctcatct ccgctcccgg
     1141 gccggaagaa atgctcgaat ccggcgaccg gctggtcttc gccggcatcg tggaatcggt
     1201 tgccgatctg cagaagatgc gcgggctcgt gcccgcgacc gggcaggtct tcaagctcga
     1261 cacgccgcgc ccggatcgcc gcctcatcga ggccgtggtc gcgccggaaa atccggtcgt
     1321 cgggcgcacc gtgcgcgagg gcggcttccg ctcgcgctac ggcgccgtcg tgatcgccgt
     1381 cgcccgtcgc gggcaacgca tcaaacgcaa gatcggcgag atcgcgctcg agcccggcga
     1441 cacgctcctg ctcgaggcgc ccgcggagtt tctgcgccgg taccgtcatc accgcgactt
     1501 tctgctgctc cgtccgctcg agggcccggt gcaaccgcat tacgaacgcg cctggatcgc
     1561 ctgggcgatt ctcggcgcca tgatcggctg cgtcaccgcc gggctcgtgc cgctcgcccc
     1621 ggccgcggtc ttcgccgccg ttctcatggt cgcgacccgc tgcatcgggc tcaacgcggc
     1681 gcgccgcgcg atcgagctgc aggtgatcct catcatcggc gcctcgtttg cgatcggcgc
     1741 cgcactcgtg catacgggag cggcggaggc gattgcccgc ccgatgctcg cgcttgcgca
     1801 ggactcgccg ctcgggctgc tcgcgatgat ctacgcgatc accaccattc tcacctcctt
     1861 catcagtcac aacgccgccg ccgtgctcat gttcccgctg atgctcgccg ccgtcggcgc
     1921 gctcggccag ccggtgctgc ccttcgcgat cgcgatcgcg atggcggcct cggcgagctt
     1981 cgcgactccg atcagctacc agaccaatct catggtctac ggccccggcg gctatcagtt
     2041 cctcgatttc gtgcgcttcg gcctgccgct caacgtggtc gttggagccg tttcgatctt
     2101 cgtgatccat caggtctggc tcgcctgagc cgcggtccgc gtccgacccg cgctgtccgg
     2161 gaaacgtcac gatttcgttt cgctcttgtg tgcgcgagcg cacggtaacc gcggcgcgca
     2221 gcgggtgaaa taacccgacg cccacgggtg cgggttcact cgccatgccg agcgcaggta
     2281 tcgtttccct gtcccttcct gagtcgaccg cggacgacgg gagcgcggcg gtgcagatcg
     2341 gcgatgatcg ctatcgcagt ctcttcgcga ccatcgacga aggactctgc gtcctggaag
     2401 tggaatacgg cggagacggg aacatcgccg atctccgttt ccgcgaggtg aacgaggcct
     2461 tcgagcgcca gagcggcatg cgtgaggttc tcggaaaacc gctcagccgc ttcctgcccg
     2521 atttcgcgcg gcagcgggcc ggcgccatcg cccgggtcct ggagacgggc gggccgttgc
     2581 gcctggaaaa ctttcacgcc gacacggggc gctggtaccg cgtgcagtat tcgcgcatcg
     2641 gcggcgagcg cagtcgtctc ctcgccgcgg tcttcgagga cgtgaccgaa cgcaaattcg
     2701 ccgagcgcac ggcaaatcgc ctcgccgcga tcgtcgattc ctccgacgat gcgatcatca
     2761 gcaaggatct cgacggctca tccagacctg gaacgcgggc gcggagcgca tcttcggcta
     2821 ccgcgcggaa gaggccatcg ggcgcccggt cacgatgctg atgccgcccg atcgcgtgga
     2881 cgaggagccc ggcatcctcg cgcgcatccg ccgcggcgag cgtatcgacc attacgaaac
     2941 ggtgcgccag cgcaaggacg gcacgctcct cgacatctcg ctcagcgtct cccccatctt
     3001 cgatcgcgag
//
LOCUS       DASUJG010000104         3114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_641125, whole genome shotgun sequence.
ACCESSION   DASUJG010000104 DASUJG010000000
VERSION     DASUJG010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641125"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..160)
                     /gene="rpsE"
                     /locus_tag="VFX38_02065"
                     /pseudo
     CDS             complement(1..160)
                     /gene="rpsE"
                     /locus_tag="VFX38_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005001261.1"
                     /note="located at the back of the 30S subunit body where
                     it stabilizes the conformation of the head with respect to
                     the body; contacts S4 and S8; with S4 and S12 plays a role
                     in translational accuracy; mutations in this gene result
                     in spectinomycin resistance; internal stop; incomplete;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
     gene            complement(170..532)
                     /gene="rplR"
                     /locus_tag="VFX38_02070"
     CDS             complement(170..532)
                     /gene="rplR"
                     /locus_tag="VFX38_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX5313681.1"
                     /translation="MRHKEQSRERRSRRTRANIRTVEAVRLTVHRTPRHMYAQLISPG
                     GATTLAAASTLDAELRESLKDKGTGNIAAAVAVGKLIAERGKGAGVSRVAFDRSGYKY
                     HGRVRALADAAREAGLEF"
     gene            complement(529..1062)
                     /gene="rplF"
                     /locus_tag="VFX38_02075"
     CDS             complement(529..1062)
                     /gene="rplF"
                     /locus_tag="VFX38_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX5313682.1"
                     /translation="MARTAVKPIAIAENVEVSIERQRIRIEGAKGTLELEVNSQVAVE
                     REEQTLAFSPRNGGKDAVAHCGTARALVRNLMRGVVSGYERKLELNGVGFRAQAQGRK
                     LDLTLGFSHPVQFVAPEGIALETPSQTEIFVRGIDKQLVGEVAAKIRRFRPPDAYKGK
                     GLRYADERIVLKEAKKK"
     gene            complement(1066..1464)
                     /gene="rpsH"
                     /locus_tag="VFX38_02080"
     CDS             complement(1066..1464)
                     /gene="rpsH"
                     /locus_tag="VFX38_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX5313683.1"
                     /translation="MSMHDPVADFLTCIRNAQGAGHPEVTVPASRLKRELARVLKEEG
                     YIEDFRDGAGDEGKPALVVRLKYYEGRPVIERLRRVSRPGLRVFRAKDELPRVSGGYG
                     VAIVSTSRGLMSDRAARAAGHGGEVLCEVS"
     gene            complement(1475..1780)
                     /gene="rpsN"
                     /locus_tag="VFX38_02085"
     CDS             complement(1475..1780)
                     /gene="rpsN"
                     /locus_tag="VFX38_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005408209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEX5313684.1"
                     /translation="MAKKSMIARDEKRVELAAKYTKKRAELRRRIRDPQAGLAERMEA
                     AQALADLPRDSSPSRQRRRCKLTGRSRGTYRKFGLAHSKVREVVMRGDAPGVSMASW"
     gene            complement(1790..2329)
                     /gene="rplE"
                     /locus_tag="VFX38_02090"
     CDS             complement(1790..2329)
                     /gene="rplE"
                     /locus_tag="VFX38_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018941450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX5313685.1"
                     /translation="MTGLKQHYEKTIMPALTERFGYGNRFEVPRLMKITLNMGVGEAA
                     ADRKVIENAAADLTLIGAQKPLVTRTRKAVAGFKIRENWPIGCKATLRRERMYDFLQR
                     LIHVAIPRIRDFRGFSARAFDGRGNYSLGIREQIIFPEIDYDKIDAIRGLDVTLTTSA
                     RTDEEGRALLEAFSFPFRN"
     gene            complement(2340..2657)
                     /gene="rplX"
                     /locus_tag="VFX38_02095"
     CDS             complement(2340..2657)
                     /gene="rplX"
                     /locus_tag="VFX38_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017840750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEX5313686.1"
                     /translation="MERIKKGDEIIVIAGKDKGRRGTVTRTYRDGRVLVEGINMVRKH
                     MKPNPQTGSKGGIVDREMPLHASNVLHYNGVTRKGDRIGTKRLEDGRKVRFFRSTDEL
                     VDI"
     gene            complement(2738..3106)
                     /gene="rplN"
                     /locus_tag="VFX38_02100"
     CDS             complement(2738..3106)
                     /gene="rplN"
                     /locus_tag="VFX38_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004145357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEX5313687.1"
                     /translation="MIQMQSELDAADNSGARRLRCIKVLGGSKRRYAGIGDVIKVSVA
                     DAIPRGRVKKGEVYDAVVVRTRKGLRRADGSAIRFDGNAAVLLNNRLEPIGTRIFGPV
                     TRELRTERFMRIISLAPEVL"
BASE COUNT          487 a         1089 c          933 g          605 t
ORIGIN      
        1 gggccttccc gtagccgaag cccacgcgtc cggcgccgtc gcccacgacc atgagcgccg
       61 tgaatccgaa ctggcggccg cccttgacga cctacgcgac ccgattgccc gcgaccagtt
      121 tctccagcgg atcgcccgtt ccttccattg tctttggcat aaggctacct tagaactcga
      181 ggcccgcctc gcgcgccgca tcggcgagcg cgcgaacgcg accgtgatat ttgtagcccg
      241 aacggtcgaa ggccacgcga gagactcccg cgccctttcc ccgctcggcg atgagcttgc
      301 cgaccgccac ggcggccgca atgtttcccg tccccttgtc cttcagggac tcgcgcagtt
      361 ccgcatccag cgtcgaggcg gccgcgagcg tcgtcgcgcc acccggcgaa atcagctggg
      421 cgtacatgtg gcgcggcgta cgatgcacgg tgaggcgcac cgcctccacc gttcggatgt
      481 tcgctcgcgt gcgccgggag cgccgctcgc gcgactgttc cttgtgtctc atttcttctt
      541 cgcctccttg agcacgatgc gctcatcggc gtagcgcaaa cccttgcctt tgtacgcatc
      601 gggcgggcgg aaccgcctga ttttggcggc gacctcgccg accagctgct tgtcgattcc
      661 gcgcacgaat atctcggtct ggctcggtgt ttccagagcg atcccttccg gggccacgaa
      721 ctgcacggga tgcgaaaatc ccaacgtgag atcgagcttg cggccctgcg cctgggcgcg
      781 gaaccccacg ccgttcaact ccagcttgcg ttcgtagccg gagacgacgc cccgcatcag
      841 gttgcgcacg agcgcccgcg ccgtgccgca gtgagcgacg gcgtccttgc cgccgttcct
      901 ggggctgaag gcgagcgtct gctcctcccg ttctacggcg acctgcgaat tcacctcgag
      961 ttccagcgtg cctttcgcgc cctcgatgcg gatgcgctgg cgctcgatgc tgacctcgac
     1021 gttttccgcg atcgcgatcg gtttgactgc cgtgcgtgcc atggatcaac tcacctcgca
     1081 gagaacttcg ccgccgtggc cggccgcgcg cgccgcgcga tcgctcatca ggccccgcga
     1141 agtcgaaacg atggcgacgc catagccgcc cgatactcga ggcagttcgt ccttggcgcg
     1201 aaagacgcgc aggcccgggc ggctcacgcg ccgcagccgc tcgatcaccg gccgcccctc
     1261 gtaatacttg agccggacca caagcgcggg tttcccctcg tctcccgcgc cgtcgcgaaa
     1321 atcttcgatg tacccttctt ccttcagcac ccgcgcgagt tcgcgcttca gtcgggaggc
     1381 cgggaccgtc acctcgggat gaccggcgcc ctgcgcattg cgaatgcagg taaggaagtc
     1441 ggcaaccgga tcgtgcatgc tcatgcgcgg gacctcacca gctcgccatc gagacgccgg
     1501 gcgcatcgcc gcgcatcacg acctcgcgaa ccttgctgtg cgccagcccg aacttgcggt
     1561 aggtgccacg cgaacggccg gtgagtttgc agcggcggcg ctggcggctc gggctcgaat
     1621 cgcggggaag atccgccagc gcctgcgcgg cttccatccg ttccgcgagg cccgcctgcg
     1681 ggtcgcggat acgccgccga agctccgcgc gctttttcgt atatttcgcg gccagttcga
     1741 cccgcttctc gtcgcgcgcg atcatggatt ttttcgccat ctctacacct cagtttcgga
     1801 acggaaagga aaatgcctcg agcagggcac gtccttcctc gtcggttctc gcgctggtcg
     1861 tgagcgtcac gtccaggccg cgaatcgcgt cgatcttgtc ataatcgatc tcggggaaaa
     1921 tgatctgctc gcggatcccg agactgtagt ttccgcgccc gtcgaacgcg cgggcggaga
     1981 agccgcgaaa gtcgcggatg cgcggaatcg ccacatggat caaccgctgc aggaaatcgt
     2041 acatgcgctc gcggcgcagc gtcgccttgc agccgatcgg ccagttctcg cggatcttga
     2101 atccggcgac ggccttgcgc gtacgtgtaa cgagcggctt ctgagcgccg atcagggtga
     2161 gatcggccgc cgcgttttcg atcaccttgc gatcggccgc cgcttcgccg acacccatgt
     2221 tgagcgtaat cttcatcagg cgcggaacct cgaagcgatt gccatagcca aagcgctccg
     2281 tgagcgccgg catgatcgtc ttctcgtagt gttgcttcaa acccgtcatg acttcggcct
     2341 cagatgtcca ccagttcgtc ggtggaacgg aagaatctca ccttgcgccc gtcctccagg
     2401 cgcttggtcc cgatgcggtc tcccttgcgg gtgacgccgt tgtagtgcag gacattagaa
     2461 gcatgcaaag gcatctcgcg atccacgatt ccgcccttgc taccggtctg gggattcggc
     2521 ttcatgtgct tgcgcaccat gttgatgcct tccaccaata cgcggccgtc gcgataggtc
     2581 cgcgtgaccg tgccgcgccg gcccttgtcc ttgccggcga tgacaatgat ctcgtcgccc
     2641 ttcttgatcc gttccataac aactactcgt cagccattcg cggcggggac cgccgctcct
     2701 gcaatctcgc tgctcactgc tctcacctcg cttctcttca caggacttcc ggcgcgagcg
     2761 agatgatgcg catgaagcgc tccgttctca gctcccgcgt caccggcccg aagattcggg
     2821 tcccgatcgg ctccaggcgg ttattcagga gcaccgccgc attgccgtcg aaacgaatcg
     2881 ccgagccgtc ggcgcggcga agacccttgc gggtgcgaac caccacggcg tcgtacacct
     2941 cgcccttttt cacgcgtcca cgcggaatgg cgtcggcaac gctcactttg atcacgtcac
     3001 cgattcccgc gtagcgccgc ttcgaaccgc ccagcacctt gatgcagcgc agacggcggg
     3061 cgccgctgtt gtcggcggca tcgagttcgc tctgcatctg gatcatggcg aggg
//
LOCUS       DASUJG010000105         2521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1375950, whole genome shotgun sequence.
ACCESSION   DASUJG010000105 DASUJG010000000
VERSION     DASUJG010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375950"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..1822
                     /locus_tag="VFX38_02105"
     CDS             221..1822
                     /locus_tag="VFX38_02105"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX5313688.1"
                     /translation="MIAQQQGKSVEPSQLVVELSRWQFAVTVLYHFLFVPLTIGLGVL
                     LAAMETVYVVTRREIFRDMTKFWSKLFVINFALGVATGLTMEFQFGTNWAFYSHFVGD
                     VFGAPLAIESLMAFFLESTLVGIMIFGWDRLSRGKHLFVTYMVALGANLSALWILIAN
                     GFMQHPIGAHLDPATLRMQLSSFSQLIFSIDAQAKFVHTTAASYVLGSMFVCAVSAFY
                     LLRRRHVEIARRSFRMAAIFGAFASIAVITLGDASGFVDVGVQPPKIAAMEAMWHTEK
                     PPAGLNLIAIPSEREERNLWELKFPAIGGLMLTHGFTEPVLGVKELIAQARQKVRDGI
                     PALLALHELQKNPTDAAALATYKAHQKNIGYALLLQRYAPDVSKATPAQVERAAKDTI
                     PLVWAIFWTFHLMVYFGLAMFAFFVLASMFSLTRSEYRRPWLLRLAIFMPVLPWLTVE
                     LGWITAELGRQPWTVYGILPTWLSASTHSVAYMIFSLTGFVLLYTIFIVIEVYLMGRA
                     IRKGPEPELSDEEAAGPPQLVPAGD"
     gene            1836..2033
                     /locus_tag="VFX38_02110"
     CDS             1836..2033
                     /locus_tag="VFX38_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019092292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313689.1"
                     /translation="MSLSELLRYAGWLILAALALAAAAMLGYSAPWYFAWAIGTAMII
                     LIASFAGALYDRQARDEGEED"
     gene            2036..>2521
                     /gene="cydB"
                     /locus_tag="VFX38_02115"
     CDS             2036..>2521
                     /gene="cydB"
                     /locus_tag="VFX38_02115"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX5313690.1"
                     /translation="MEAYAVLKIVWWLLLGVLFVGIGIMIGQDMGIGTSLRYLGRSEH
                     ERRAVLTMIGPHWGGNGVWFVLGGGALFAAFPTVYATLFSGLYIVMLLLLWAMIVRPL
                     GFEYRSKLESRRWRGTWDLALFASGFVPMLIFGTAIGNALRGFPFRFNDLLQSFYPNG
                     FA"
BASE COUNT          401 a          807 c          763 g          550 t
ORIGIN      
        1 tatggccgac tatctcgcct gagcgtttgc gactcgcttc gacgtcaacg tgacggcggg
       61 ttccagcaca cggcattcct tcggaacgca gcgtgactga ttgcggtctc gatccgggaa
      121 ggccccggtc gttgcgccaa ctcgatgccg gcaatcgctt ccgcaaggcc ctgcgcgaaa
      181 cctgatccag atcaattcgc cgggcgctcg ggcgactata atgatcgcgc agcagcaggg
      241 gaaatcggtg gaaccgagcc aactggtggt cgagctttcg cgctggcagt tcgccgtgac
      301 cgtgctctac cacttcctct tcgtgccgct caccatcggt ctcggcgtac tcctcgccgc
      361 gatggaaacc gtgtacgtcg taacgcggcg tgaaatcttc cgcgacatga cgaagttctg
      421 gagcaagctg ttcgtcatca acttcgccct cggcgtcgcg accgggctca cgatggagtt
      481 ccaattcgga accaactggg cgttctattc gcatttcgtc ggcgacgtct tcggcgcacc
      541 gctcgcgatc gaatccctga tggcattttt tctcgagtcc acgctcgtcg gcatcatgat
      601 cttcggctgg gaccgactgt cgcgcggcaa gcatctgttc gtcacctaca tggtggcgct
      661 tggtgccaat ctctcggcgc tgtggatcct catcgcgaac ggcttcatgc agcatccgat
      721 cggcgcgcat ctcgatcccg cgacgctcag gatgcagctt tcgagtttct cgcaactcat
      781 cttcagcatc gacgcccagg cgaagttcgt gcacacgacg gccgcaagct acgtcctggg
      841 ctcgatgttc gtctgcgccg tgagcgcgtt ttatcttttg cgcaggcgcc acgtcgagat
      901 cgcgcgccgg tcttttcgca tggcggcgat attcggcgcc ttcgcctcca tcgcggtgat
      961 tacgctcggc gacgcctctg gtttcgtgga cgtgggagtt cagcctccca agattgcggc
     1021 gatggaagcc atgtggcata cggagaagcc gcccgccggg ctcaatctca tcgccattcc
     1081 gagcgagcgc gaagaacgca atctctggga gctgaagttt cctgcgatcg gcgggctgat
     1141 gctcacgcac ggtttcaccg agccggttct gggcgtgaag gaactcatcg cccaggcgcg
     1201 gcagaaggtg cgcgacggca tacccgcgct gctcgcactg cacgagctgc agaaaaaccc
     1261 gaccgatgca gcggcgctcg caacgtacaa ggcgcaccaa aagaacattg gttacgccct
     1321 tctgcttcag cgctatgcgc ccgacgtctc gaaagccact ccggcgcagg tcgagcgggc
     1381 ggcgaaagat actattccgc tggtgtgggc catcttctgg acctttcatc tgatggtcta
     1441 cttcggtctg gcgatgttcg cctttttcgt gctcgcctcg atgttttcgc tcacgcgcag
     1501 cgagtatcgc cgaccctggc tgctgcgcct tgcgatcttt atgccggtgc tgccctggct
     1561 tacggtggaa ctcggctgga tcaccgcgga actcggacgc cagccgtgga cggtctacgg
     1621 cattcttccg acctggctgt cggcctcgac ccacagcgtc gcctacatga tcttttcgct
     1681 caccggcttc gtgctgctct acacgatctt catcgtgatc gaggtctatc tcatgggccg
     1741 ggcgatacgc aagggtccgg agcccgaact cagcgacgag gaagcggcgg gtccgccgca
     1801 actcgtgccc gcgggcgact gaggcggagt atgcaatgag tctttcggaa cttcttcgtt
     1861 acgccggctg gctgattctc gccgccctcg cgctcgcggc cgcggcgatg ctcggctact
     1921 cggcgccctg gtactttgcc tgggcgatcg gcacggcaat gatcatcctg atcgcgtcct
     1981 ttgcgggcgc gctctatgac cgccaggcgc gcgacgaggg cgaagaggac tgagcatgga
     2041 agcctatgcc gtactgaaaa tcgtctggtg gctgcttctc ggcgtccttt tcgtcggcat
     2101 cggcatcatg atcggacagg acatgggcat cggcacttcg ctgcgctatc tcgggcgcag
     2161 cgagcacgaa aggcgcgccg tgctcacgat gatcgggccg cactggggtg gcaacggcgt
     2221 gtggttcgtg ctcggaggcg gcgcgctttt cgcggccttt ccgaccgtct acgcgacact
     2281 tttttcggga ctctacatcg tcatgctgct gctcctctgg gcgatgatcg tgcgcccgct
     2341 cggcttcgag taccgcagca agctcgagtc gcggcgctgg cgcgggacct gggatctggc
     2401 gctctttgcc tcgggcttcg tgccgatgct catcttcggc accgcaatcg gcaacgcgct
     2461 caggggcttt ccgttccgct tcaacgatct cctgcagtcg ttctatccga acggcttcgc
     2521 c
//
LOCUS       DASUJG010000106         4016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_519756, whole genome shotgun sequence.
ACCESSION   DASUJG010000106 DASUJG010000000
VERSION     DASUJG010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519756"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VFX38_02120"
     CDS             <1..480
                     /locus_tag="VFX38_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX5313691.1"
                     /translation="IGGDRFDEAILNYVRRNYSILIGEATAERIKIQIGSAFPGREVR
                     EMEVKGRNLAEGVPRSFTLNSNEILEALQEPLAGIVGAVKQALEQTPPELAGDVAERG
                     MVLSGGGALLRDLDRLITEETGIAVIIAEDPLTCVARGGGRALEIIDERGPDLFALE"
     gene            722..1579
                     /gene="mreC"
                     /locus_tag="VFX38_02125"
     CDS             722..1579
                     /gene="mreC"
                     /locus_tag="VFX38_02125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX5313692.1"
                     /translation="MLRFTRERRLFHPARGQALRSFVLALAAIALMTVDYHYGTLAVV
                     RDSLSVVVYPAKWLVNAPSAAWREVSTDLSGRVHLLAENRRLHRELLLAQSRLERLDS
                     LAEQNRRLQMLLNAAAAMPGRVLATSVLEVDLNPFENVIVVNAGQGSGVRKGQPVLDA
                     YGIVGQVLDAGPLSSRVLLITDPASAVPVEIERTGLATLALGTGNLDRLSLPYLPNNT
                     DVRPGDHLLSSGLGGRYPRGYPVGIVISVKSQPEQRFAQVIARPFAHLGREHEMLIYS
                     SGSEPKAPP"
     gene            1579..2061
                     /gene="mreD"
                     /locus_tag="VFX38_02130"
     CDS             1579..2061
                     /gene="mreD"
                     /locus_tag="VFX38_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX5313693.1"
                     /translation="MRKLSFALTIVLSLLAAFILTIVPWPLWAEPLRPLWVALTVFYW
                     IVALPERLGVVFAWIVGLFLDVLTGTVLGAHALALSLVAFIAARSHLKFRMYPLWQQS
                     LTVGLALALYAFVLFWIGGLTGAMTLPVVRFVPVAVSMLLWPCVYLSLRALRRRFVPA
                     "
     gene            2065..3942
                     /gene="mrdA"
                     /locus_tag="VFX38_02135"
     CDS             2065..3942
                     /gene="mrdA"
                     /locus_tag="VFX38_02135"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX5313694.1"
                     /translation="MSRPIPIGAGHERFARRLLLVAAVALVLGAGLLARFVWLQIVEA
                     PYYGALALGNRTRTVPLAPTRGIIYDRNGVVLAENIPTYEITLTPDQVTNVAAALARL
                     GRIIPISAQDRDNFRKLRATKKNFQPVPVKTDLTPAEVARFAVNRQDFPGLDVTATLK
                     RYYPPGTDATDVVGYVGLMSNEDLKRLDANEYAASARVGKIGVEWQYERLLHGSIGYQ
                     KVEVNAVGRAIRVLATRQPRPGEDLYLTIDTRLQRIAYRALGARAGAVVAIQPASGAV
                     LCMVSKPAFDPNLFVTGISQKDYDALLSRPGDPLVNRALRGRYAPGSTIKPFLGLAAL
                     YYGVLTPATRLFAGPTFRLPNYSHVFHDWNPYQSGYETLEQALIRSTDTFFYQVAQRL
                     GIQRMHDFLAHFGFGEKPPIDLPGALAGTNPSPAWKRATYGKPWYRGETINNGIGQGY
                     FQITPLQLAWATAVLAARGKRYPPHVLGASAIPGSDKRTDYAGDPLAPLRLPERADWK
                     YVVHALHEVVANPRGTAHGIAKGLDFPLAGKTGTAQVTSIYHSDFAKEENIPLKQRDN
                     ALFIGFAPVQNPQIAVAVIVEHGGGGSSAAAPIARKIIAAWIHRNDPAGKTHDDAQPS
                     H"
BASE COUNT          623 a         1384 c         1284 g          725 t
ORIGIN      
        1 atcggcggcg atcgcttcga cgaggccatc ctgaattacg tccggcgcaa ctattcgatc
       61 ctgatcggcg aggcgacggc cgagcgcatc aagatccaga tcggttcggc ctttcccggg
      121 cgcgaggtgc gggagatgga agtcaagggc cgcaacctcg ccgagggggt tccgcgcagc
      181 ttcacgctca attccaacga gattctcgag gccctgcagg agccgctcgc cggcatcgtg
      241 ggcgcggtca aacaggcgct cgaacaaaca cctcccgagc tcgccggcga cgtcgccgag
      301 cgggggatgg tgctttctgg aggcggcgcg ttgctccgcg atctcgatcg cctgatcacc
      361 gaggagacgg ggatcgccgt gatcatcgcc gaggatccgc tcacctgcgt cgcccgcggc
      421 ggcgggcggg cgctcgaaat catcgacgag cgcgggccgg atctgttcgc gctcgagtag
      481 aagtcccgcg cgcaggggcc tttccggccg ttcgaagtgt tttccggttt gcatgtaaac
      541 tgcgggcgct acgctcggct cgcgtcgcgg gcgttgattc cgcccgcagg tgcatgggag
      601 gcgcggccgg gcgagcaggc accggaacca cgacccgaca ggtatcgatg aacgagcgcc
      661 cccgcgcggt cgcgccgcga tctcccgcca ccgccgcgag gcgttcgcgg cgccgcccgg
      721 catgctgaga ttcacgcgcg aacgccgctt gtttcacccg gcgcgcgggc aggcgctgcg
      781 cagtttcgtc ctcgcactcg cggcgatcgc gctcatgacc gtggactatc actacggcac
      841 gctcgcggtc gtgcgcgaca gcctgagcgt cgtggtgtat cccgcgaagt ggctggtgaa
      901 cgcgcccagc gccgcctggc gcgaggtttc gaccgacctt tccggtcgcg tccatctgct
      961 cgcggaaaac cggcgtctgc accgggagtt gttgctcgcg cagtcgcggc tcgaacgtct
     1021 cgattcgctc gccgagcaaa accgccgcct gcagatgctg ctcaatgccg ccgcggccat
     1081 gccggggcgg gttcttgcga cgagcgtgct cgaagtggat ctcaatccct tcgaaaatgt
     1141 gatcgtcgtc aacgccgggc agggcagcgg cgtgcgcaag ggccagccgg ttcttgatgc
     1201 ctacggcatc gtcggccagg tgctcgacgc cgggcccctg agctcgcgcg ttctcctcat
     1261 caccgatccg gcgagcgccg taccggtcga gatcgaaaga acggggcttg cgaccctcgc
     1321 tctgggcacc ggaaatctcg atcggctgag ccttccctat ctgcccaaca ataccgacgt
     1381 gcgtcccggc gaccatctgt tgagttcggg actcggcggg cgctacccgc gaggctatcc
     1441 ggtcggcatc gtgatttcgg tgaaatcgca gccggagcag cgtttcgcgc aggtcatcgc
     1501 ccggccgttc gcccatctcg ggcgcgaaca cgagatgctg atctattcga gcggcagcga
     1561 gccgaaggcg ccgccgtgat gcgcaagctg agcttcgcgc tcacgatcgt gctgagtctc
     1621 ctcgccgcct tcatcctcac gatcgttccc tggccgctgt gggcggaacc gctgcgccca
     1681 ctgtgggtcg ccttgaccgt gttctactgg attgtggcgt tgccggaacg cctcggcgtc
     1741 gtgttcgcct ggatcgtcgg gctttttctc gatgtcctca cgggcacggt tctcggtgcg
     1801 cacgcgctcg ccctcagcct cgtcgccttc atcgcggcgc gcagccactt gaagttccgc
     1861 atgtatccgc tctggcagca gagccttacc gtcggcctcg cgctcgccct ttatgccttc
     1921 gtgctcttct ggatcggcgg gcttaccggc gcgatgacgc tccccgtcgt gcgcttcgtt
     1981 ccggtcgcgg taagcatgct gctgtggccc tgcgtgtatc tttccctgcg ggcgctgcgg
     2041 cgccgctttg ttccggcctg agacgtgagc cgaccgattc cgatcggcgc cggccatgag
     2101 cgttttgcgc gccggcttct cctggtcgcc gccgtcgcgc tcgtgctcgg cgccggactg
     2161 ctcgcgcgct tcgtgtggct gcaaatcgtg gaggcgccct attacggtgc tctcgcgctc
     2221 ggcaaccgca cgcgcaccgt tccgctcgcg cctacgcgcg ggatcattta cgaccgcaac
     2281 ggcgtggtgc tcgcggagaa tattcccacg tatgaaatca ccctgacccc ggatcaggtg
     2341 acgaacgtcg ccgcggcgct cgcgcgcctc gggcggatca ttccgatcag cgcccaggat
     2401 cgcgacaatt tccgcaaact gcgggcgacc aagaagaact ttcagccggt gccggtcaag
     2461 accgatctca ccccggcgga ggtcgcgcgc ttcgccgtaa atcgtcagga tttccccggg
     2521 ctcgacgtga ccgcgacgct gaagcgctac tacccgccgg gcaccgacgc caccgacgtc
     2581 gtcggctatg tcggcctgat gagcaacgag gacctcaagc ggctcgatgc gaacgaatat
     2641 gccgcgagcg cgagggtggg gaaaatcggc gtcgaatggc aatacgagcg tctgcttcac
     2701 ggcagcatcg gttaccagaa ggtcgaggtg aatgcggtcg ggcgcgcgat ccgggtgctt
     2761 gcgacccgcc agccgcgccc cggcgaagat ctgtacctca cgatcgacac gcggcttcag
     2821 cgcatcgcct accgcgcgct cggtgcgcgc gcgggagccg tcgtcgcgat ccagccggcg
     2881 agcggtgccg tgctctgcat ggtgagcaaa cccgccttcg atccaaatct tttcgtgacc
     2941 ggaatttcgc agaaggacta cgacgcgctg ctcagccgtc cgggcgatcc actcgtgaat
     3001 cgggcgctgc gcgggcgcta tgcgcccggc tccacgatca aaccctttct cgggctcgcc
     3061 gccctctatt acggcgtcct cacgccggca actcggctgt ttgcggggcc cacgttccgg
     3121 ctgccgaatt actcgcatgt gttccacgac tggaatccgt accagagcgg ctacgaaacc
     3181 ctggagcagg cgctgatccg ttcgaccgac accttttttt atcaggttgc acagcggctc
     3241 ggcatccagc ggatgcacga ctttctcgcg catttcggtt tcggcgagaa accgccgatc
     3301 gatctgccgg gagcgctcgc cggcaccaat ccttcgcctg cgtggaaacg ggccacctac
     3361 ggcaagccct ggtaccgcgg cgagacgatc aacaacggaa tcggccaggg atatttccag
     3421 atcacgccgc tgcaacttgc ctgggcgacg gccgttctcg ccgcccgcgg caagcgctac
     3481 ccgccgcacg tgcttggcgc gagcgccatt ccgggaagcg acaagcggac ggattacgcc
     3541 ggcgatccgc tggctcccct gcggcttccg gagcgcgccg actggaagta cgtcgttcac
     3601 gcgctccatg aggtggtcgc caaccctcgc ggcaccgccc acggcatcgc caaggggctc
     3661 gattttcctc ttgccggaaa aacgggcacc gcgcaggtaa cgagcatcta ccacagcgat
     3721 ttcgccaagg aggaaaacat cccgctcaag cagcgcgaca atgcgctgtt catcggcttc
     3781 gcgccggtac agaatcccca gatcgcggtc gcggtgatcg tcgagcacgg cggcggcggc
     3841 tcgagcgccg cggcgcccat cgcccgcaag atcatcgcgg cctggatcca tcgcaacgat
     3901 ccggcaggaa agacccacga cgatgctcag ccgtcgcatt gacccgccgc ttctctccgc
     3961 gctgctgctg ctcgcgctcg gcggtcttgc gctcctctat tccgcgagcg gtggcg
//
LOCUS       DASUJG010000107         2202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1743236, whole genome shotgun sequence.
ACCESSION   DASUJG010000107 DASUJG010000000
VERSION     DASUJG010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1743236"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1288
                     /gene="trmFO"
                     /locus_tag="VFX38_02140"
     CDS             <1..1288
                     /gene="trmFO"
                     /locus_tag="VFX38_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEX5313695.1"
                     /translation="QLAEAGLRVRLSEMRGGGDMTPAHETDRLAEMVCSNSFRSDDSD
                     HNAVGLLHQEMRALDSLIMRAADVHRVPAGSALAVDRDGFAAEVTRAIAEHPKISVIR
                     ERVDALPDHPTIVATGPLTGSKLAEAIATETGQGALAFFDAIAPIVHRDSIDMDVAWM
                     AARWDKGGKDYINCPLDKLQYEDFVAALIDGEKMPFHDWEKDTPYFEGCMPIEVMAER
                     GPETLRHGPMKPVGLDNPRTGRWPYAVVQLRQDNALGTLWNMVGFQTKLKHGEQVRIF
                     RTIPGLENAEFARLGGIHRNSFINSPRLLDRELRLKSKPNIRFAGQITGCEGYVESAA
                     VGILAARFAVAEVKGQSLDEGQSLPPPETAIGALLNHITGGADAETFQPMNVNFGLMP
                     PLDSRARKADRKKAYTDRARAAFGRWMEHVQVNEPA"
     gene            complement(1285..1740)
                     /locus_tag="VFX38_02145"
     CDS             complement(1285..1740)
                     /locus_tag="VFX38_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX5313696.1"
                     /translation="MLRFFAGAAACFLLMTGAFLIWQSRAEQGPGIPGAPPARTYSAS
                     MLQAPEASTKSREERRFGRADKNKDGKIESEELLGSRRKAFAKLDANGNGSLAFEEWA
                     HTTIDKFKGADKDRNSLLTAAEYATTAPPPPKHRTCSCGVPGPIARSDD"
     gene            complement(1810..>2202)
                     /locus_tag="VFX38_02150"
     CDS             complement(1810..>2202)
                     /locus_tag="VFX38_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313697.1"
                     /translation="GWLRLFDPFPWTIETSEAIWLRGNLLFGLGARVLGQAGEPIQAA
                     GGLWALVDSARHCSDRESRQMLMGQARSFAKRLGGARFAAHLRPLSMLAALAVRDCRH
                     GEPFEREGTAGHAAAMLRHRLGGRIPRL"
BASE COUNT          386 a          782 c          696 g          338 t
ORIGIN      
        1 gcagctcgcg gaagcagggc ttcgcgtgcg cctcagcgaa atgcgcggcg gcggcgacat
       61 gactccggcg cacgagaccg accggctcgc cgaaatggtc tgttcgaaca gcttccgctc
      121 ggacgattcg gaccacaacg ccgtcggcct gcttcaccag gagatgcgcg cgctggattc
      181 gctgatcatg cgcgcggcgg acgtgcaccg agtcccggcc gggtccgcgc tcgcggtcga
      241 tcgcgacggc ttcgcggcgg aggtaacgcg cgcgatcgcc gagcacccaa aaatcagcgt
      301 aattcgcgag cgtgtagacg cgcttcccga ccaccccacg atcgtcgcga ccggcccgct
      361 gaccggatcg aagcttgccg aagccattgc cactgaaacg ggccagggcg ccctcgcctt
      421 cttcgatgcc atcgccccca tcgtccatcg cgacagcatc gacatggacg tcgcctggat
      481 ggccgcgcgc tgggataagg gcggcaagga ctacatcaac tgcccgctcg acaagctgca
      541 gtacgaggat ttcgtggcgg ccttgatcga tggcgagaag atgccgttcc acgactggga
      601 aaaggacacg ccctacttcg aaggctgcat gccgatcgag gtgatggccg agcgcggacc
      661 ggagacgctt cgccacggcc cgatgaagcc ggtaggcctc gacaatccgc gcacaggccg
      721 ctggccctat gccgtggtcc agctccgcca ggacaatgcg ctcggcacat tgtggaacat
      781 ggtcggcttc cagaccaagc tgaagcacgg cgaacaggtg cgcattttcc gcaccattcc
      841 cgggctggag aacgccgaat tcgcgcggct cggcggcatt caccgcaaca gcttcatcaa
      901 ttcgcccagg ctgctggacc gcgagcttcg cctgaaatcg aagccgaaca tccgctttgc
      961 cggccagatc accggctgcg aaggctatgt cgaaagcgcc gccgtcggca ttctcgccgc
     1021 ccgcttcgcc gttgccgagg tcaagggaca gtccctcgac gagggacagt ccctcccgcc
     1081 gcccgaaacc gccatcggcg ccctcttgaa ccatatcacc ggtggcgccg acgccgagac
     1141 cttccagccg atgaacgtca acttcggcct catgccgccc ctcgacagcc gcgcgcgcaa
     1201 ggccgaccgc aagaaagcct acaccgaccg cgcccgcgcg gctttcggta ggtggatgga
     1261 gcacgtccag gtcaacgaac cggcctagtc gtcactgcgc gcgattggtc ccggtacccc
     1321 gcagctgcag gtcctgtgct tcggcggcgg cggggcggtg gtcgcgtatt cggcggcggt
     1381 gaggaggctg tttctgtcct tgtcggcgcc cttgaacttg tcgatcgtcg tgtgcgccca
     1441 ttcctcgaag gccagcgaac cgttgccgtt cgcatccagc ttggcgaacg ctttccgccg
     1501 cgacccgagc agctcttcgg attcgatctt gccgtccttg ttcttgtccg cgcggccgaa
     1561 gcgccgctcc tcgcggctct tcgtgctggc ctccggagcc tgaagcatcg acgcactgta
     1621 ggtgcgcgcc ggcggagcgc cggggatccc cgggccctgc tctgcccgcg actgccagat
     1681 gaggaaggcg ccggtcatca gcagaaagca cgccgcggca ccggcgaaga aacgaagcat
     1741 cggcaaatct cctttgtcgg aggagattcg gcacgcgctt atgctcggaa accaagcggt
     1801 tcgtgtcaac tacagtcgcg gaatccgtcc gccgagccga tggcggagca tcgccgcggc
     1861 atgcccggcc gtgccctcgc gctcgaacgg ctcgccgtgg cggcaatcgc gcaccgcgag
     1921 cgccgcgagc atcgaaagcg ggcgaagatg cgccgcgaac cgcgccccgc cgagccgctt
     1981 cgcgaacgag cgcgcctggc ccatcagcat ctgccgcgac tcccgatcgc tgcaatgccg
     2041 cgccgaatcg accagggccc aaaggccgcc cgccgcctgg atcggctcgc ccgcctggcc
     2101 aagaacgcgc gccccgaggc cgaacagcag attgccgcgg agccagatcg cctcgctcgt
     2161 ctcgatcgtc cacggaaacg ggtcgaacag ccgcagccag cc
//
LOCUS       DASUJG010000108         2215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_520280, whole genome shotgun sequence.
ACCESSION   DASUJG010000108 DASUJG010000000
VERSION     DASUJG010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520280"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..154)
                     /locus_tag="VFX38_02155"
     CDS             complement(<1..154)
                     /locus_tag="VFX38_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011704884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX5313698.1"
                     /translation="MRAMMLAAGRGERLRPLTDTTPKALIEVAGRPLVLHTIERLRAA
                     GITDLVL"
     gene            complement(142..1179)
                     /locus_tag="VFX38_02160"
     CDS             complement(142..1179)
                     /locus_tag="VFX38_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX5313699.1"
                     /translation="MGIGAASTAPADTRKTALADFCAAALGLAAVALEPASADASFRR
                     YFRLRLAEGATLIAMDSPPAREALASFLAVARAFGGCGLHVPRVVAADETHGFALVED
                     LGSETYLARLRSGADPAPLYGAAIEALVRLQCLHDAGLPVYDGEQLTAETALFSDWLL
                     ERHLALKLGATERALVERSGERLSALALAQPRVAVHRDYHSRNLMIAAPLPGILDFQD
                     AVLGPVSYDLVSLLKDCYIAWPRERILAWLRRYRELAEASDIPVGRDEAEFLRWFETM
                     GMQRHLKAAGIFARLYHRDGKPDYLADIPRTLRYVVEAAEKLPEFADLGAFIAARVLP
                     ALTEAKRRCGR"
     gene            1219..>2215
                     /locus_tag="VFX38_02165"
     CDS             1219..>2215
                     /locus_tag="VFX38_02165"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HEX5313700.1"
                     /translation="MRVPRAAALMLLAAGAGALAAPAIEPGQYALCATYPRLAPPPAT
                     LPAGAAPVVVEADRGSARENGLSHFSGDVVVTQGDRRLWADVLDYDQPANRASGSGNL
                     RFASPDLSLAGARGTYDFGRDRGSFFDDVYELPGAHGRGKAARVETYASDRTGLYDFL
                     YTTCPRGNQDWGLHAQHLVLDHDTEIGYAYNAWLTFKGVPFLWTPYLSFPLTDRRKSG
                     FLAPSISQSSENGLDLEVPWYWNIAPNMDATFTPRILTRRGTMFMNTYRYLFPGSSGL
                     LHVEYLPHDRELGKPRGLFQYRDHTRLFSHWNLSTSLEFLSDDDYLRDFGTDLAQV"
BASE COUNT          360 a          754 c          733 g          368 t
ORIGIN      
        1 tcaggacgag atcggtgatt cccgccgctc gcagccgttc gatcgtgtgc agcacgagcg
       61 gcctgccggc cacttcgatg agcgccttgg gagtcgtgtc ggtgagcgga cgcaggcgct
      121 cgccgcgccc ggccgcgagc atcatcgccc gcatcgacgc ttcgcctcgg tgagtgccgg
      181 aagaacgcgc gcggcgatga aggcgcctag atcggcgaat tcgggaagct tctccgccgc
      241 ctcgacaacg tagcgcagag tgcgcggaat gtcggcgagg taatccggct tgccgtcgcg
      301 atgatacaaa cgcgcgaaga tgccggcggc cttgagatgt cgctgcatcc ccatcgtttc
      361 gaaccagcgg agaaactcgg cctcgtcgcg tcccacggga atgtcgctcg cctcggcaag
      421 ttcgcgatag cggcgcaacc acgcgagaat ccgctcgcgc ggccaggcga tgtagcagtc
      481 cttcaggagc gagaccagat cgtagctcac cggtccgaga accgcatcct gaaagtcgag
      541 aatgccggga agcggcgcgg caatcatgag attgcgcgaa tggtagtcgc gatgcacggc
      601 cacgcgcggc tgggcgagcg cgagcgcgct gaggcgctcg ccactgcgtt cgacgagcgc
      661 gcgctcggtc gcaccgagct tcagcgcaag atggcgttcg aggagccagt cggaaaagag
      721 cgcggtctcg gccgtgagct gctcgccgtc gtagacggga agcccggcgt cgtgcaggca
      781 ttgcagacga acgagcgcct cgatcgcggc gccgtagagc ggcgcgggat cggcgccgct
      841 gcgcaagcgg gcaagatacg tttcgcttcc cagatcttcc accagagcaa agccgtgagt
      901 ctcgtcggcg gccacgaccc gtgggacgtg cagcccgcat ccgccgaacg cgcgggcgac
      961 ggcgagaaag ctcgcgagcg cctcgcgcgc gggcggcgaa tccatggcga tgagggtcgc
     1021 gccttccgcg aggcgcaggc ggaaatagcg gcggaaactc gcgtcggccg aggccggctc
     1081 gagcgcgacc gcggccagtc cgagcgcagc cgcgcagaag tcggcaagcg ccgtcttgcg
     1141 cgtgtccgcc ggcgccgtgc ttgccgcccc gattcccatt gtgcgatctt accgtgcttg
     1201 gccgttcgcc ggacttccat gcgggtacct cgcgccgccg ccctgatgtt gctcgccgcc
     1261 ggcgccggcg cgctcgccgc tccggcgatc gagccggggc aatacgcgct ttgtgcgacc
     1321 tatccgcgcc tcgccccgcc gcccgcgacg ctgcccgcgg gagccgcgcc ggtcgtggtg
     1381 gaggccgatc gcggcagcgc gcgcgagaac gggctgtcgc atttctccgg cgatgtcgtg
     1441 gtcacccagg gcgaccggcg cctgtgggcc gacgtgctcg attacgacca accggccaat
     1501 cgtgcgagcg gcagcgggaa tctgcgcttc gcgagcccgg atctcagcct tgcgggcgcg
     1561 cgcgggacct acgatttcgg gcgcgatcgc gggagtttct tcgacgacgt ctacgaactg
     1621 cccggcgccc acggtcgcgg caaggcggcg cgcgtagaaa cctacgcctc ggaccgcacc
     1681 gggctctacg attttctcta caccacctgt ccgcggggca atcaggactg ggggctgcac
     1741 gcccagcatc tggtgctcga tcacgacacc gaaatcggct atgcctacaa cgcctggctg
     1801 accttcaagg gcgtgccctt tctgtggacg ccgtatctgt cctttccgct caccgatcgg
     1861 cgcaagtccg gctttctcgc gccgagtatc tcgcagtcct cggaaaacgg gctcgatctg
     1921 gaggttccct ggtattggaa catcgcgccc aacatggatg cgaccttcac gccgcgcatc
     1981 ctcacgcgcc gcggaacgat gttcatgaat acctatcgct accttttccc gggcagttcg
     2041 ggcctgcttc atgtggaata tctgccgcac gatcgcgaac tcggcaagcc gcgggggctc
     2101 ttccagtatc gcgaccatac ccggcttttt tcgcattgga atctctccac gtcgctcgaa
     2161 tttctctccg acgacgacta cctgcgcgac ttcggaaccg atctcgccca ggtct
//
LOCUS       DASUJG010000109         3012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1376936, whole genome shotgun sequence.
ACCESSION   DASUJG010000109 DASUJG010000000
VERSION     DASUJG010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1376936"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VFX38_02170"
     CDS             complement(<1..251)
                     /locus_tag="VFX38_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEX5313701.1"
                     /translation="MAWLSVLSLGVVFGDIGTSPLYAMRESFAALGHAPTRAGVLGVL
                     SLILWALILVVCVKYQIFVLRAKNRGDGGIIALTALLRNL"
     gene            432..2414
                     /locus_tag="VFX38_02175"
     CDS             432..2414
                     /locus_tag="VFX38_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016352642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX5313702.1"
                     /translation="MASGGLTSVLILLAVTVAAVWLLRRARMPAALGYLLTGVFVGPH
                     LLDWVHNTATTKGLAEFGIVFLLFTLGLEFSLPRLIAMRREVFLLGGIQVLVSLVLAA
                     VGLSFFAGAKPVVAVLLGGAVAMSSTALVAKQLGEQLELQKTHGRLAIGILLFQDLAV
                     IPLLILIPMLAGEGGSPWETIAIVIGKGLLVILAMFLAARIVVRPLMHEIARRGSSEL
                     FLLASLLITLGAAWATRGLGLSLALGAFLAGAMLGETAWRHQVEASIRPFRDILLGLF
                     FITVGMYADPRQYLGHAPEIILGTVALIVFKTAIVTAASRLLRANFTAALRTGLVLGQ
                     AGEFGLVLLALGLSHGYLVGDVAQIALNILVLSMLVSPLLVRYNADIARFLSPAARGV
                     VARDADTEALALQESHAAGHVLLAGFGRVGQNLARFLDAEGFPYVALDLDPGRVRDAH
                     DGGDPVYYGDARNPAVLVAAGIERARAVVVAYNDVNSALAVIRATREVRPDVPILART
                     SDDARLEELRAAGADEVIPETLEASLMIGSHLLATLGVPMRRILRQVSDVRAGRYSLM
                     RNVYRRSGAEALDMDHAFRGQLNAVTLVGNAHGIGRSLGALDLEALGVGVTALRRDGV
                     VGREPGPETVLREGDTLVLYGTPEEAKRAEDRLLAG"
     gene            2515..>3012
                     /locus_tag="VFX38_02180"
     CDS             2515..>3012
                     /locus_tag="VFX38_02180"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5313703.1"
                     /translation="MRFHRLVFLDLATVDRGDLDLSPIVSRTREFLPWPATAPDEVAG
                     HVAAADAIVLNKVRLGAEVFERVQPRIVCLAATGTDNVDLVAAREAGIAVANIRDYCT
                     DSVVQHVFALLLALSTRLAEHREHLQTGAWPPGGAYTLPDLPIRELGGKTLAVVGFGT
                     LGRAVA"
BASE COUNT          463 a         1058 c          949 g          542 t
ORIGIN      
        1 agattcctga gcagcgccgt gagcgcgatg atcccgccgt cgccgcgatt tttggcgcgc
       61 agcacgaaga tctgatactt gacgcaaacg acgagaatca gggcccacag aatcagcgaa
      121 agcaccccga ggacgcccgc ccgagtgggc gcatgtccca gcgcggcgaa cgattcgcgc
      181 atcgcataga gcgggctcgt gccgatgtcg ccgaacacga caccgagcga caggaccgaa
      241 agccaggcca tacgccggtg cccggcgcgc gcagcgtcgc cgccccgacc gcgcgaagcc
      301 gaaaaaggat taacgagacg catatcgact gatacgcgat gcgttcgcct gcgggctcat
      361 tcgcgggtcg cgtgatccca catcggtcag cctacagtat aagctacgcg cagagaatcc
      421 aggatccggc gatggcctct ggcggactca cgagcgtgct catcctgctc gccgtcacgg
      481 tggccgcggt atggctcctg cgccgcgcgc gcatgccggc ggctctcggt taccttctca
      541 ccggcgtctt cgtcgggccg catcttctcg actgggtgca caacaccgcg acgacgaagg
      601 gactcgccga attcggcatc gtcttcctgc tgttcacact gggactggaa ttttcgctgc
      661 cgcgcctgat cgcaatgcgt cgcgaggtgt ttctcctcgg tggcattcag gtgctcgtga
      721 gtctcgtgct ggcagcagtc ggactctcgt ttttcgccgg cgcgaagccc gtcgtggcgg
      781 tgctgctcgg cggtgcggtc gcgatgtcct ccaccgcgct cgtggcaaag cagctcggcg
      841 agcaactcga gctgcagaaa acacacggcc gtctcgcgat cggaattctg ctcttccagg
      901 acctcgcggt gatcccgctc ctcatcctca tcccgatgct cgcgggcgaa ggaggctctc
      961 cctgggaaac gatcgcaatc gtcatcggca agggcctgct cgtgattctc gcgatgtttc
     1021 tcgcggcgcg catcgtcgtg cgcccgctca tgcacgagat cgcgcgccgc ggctcctcgg
     1081 agttgttcct tctcgcatcg ctcctgatca cgctcggcgc cgcctgggcg acccgggggc
     1141 tcggcctttc cctcgcgctc ggcgccttcc tcgcgggcgc gatgctcgga gaaaccgcct
     1201 ggcgacatca ggtcgaggcg agcattcgcc cgttccgcga cattctgctc ggactctttt
     1261 tcatcaccgt gggcatgtac gccgatcccc gccagtatct cggccatgcg ccggaaatca
     1321 ttctcggcac cgttgcgctc atcgtcttca agacggcgat cgtcaccgcc gcgagtcgcc
     1381 tgcttcgagc gaacttcacc gcggcgctgc gcaccggact cgtcctcggc caggccggcg
     1441 agttcggtct cgtactgctt gccctcggac tgtcgcacgg ttatctcgtc ggcgacgttg
     1501 cacagatcgc cttgaacatt ctggtgctca gcatgctagt gagcccgcta ctggtgcgtt
     1561 acaacgcgga catcgcacgc tttctctctc cggcggccag aggcgtcgtg gcaagggacg
     1621 ccgataccga agcgctcgcg ctccaggaaa gccatgcggc ggggcacgtc ctgcttgcgg
     1681 gctttggtcg cgtcggccag aatctcgcgc gatttctcga cgcggaaggt ttcccctacg
     1741 tcgcgctcga cctcgatccc ggtcgcgtgc gcgacgctca cgacggcggc gatccggtgt
     1801 actacggaga cgcgcgcaac ccggccgtgc tcgtcgccgc cggaatcgag cgcgcccgcg
     1861 ccgtggtggt cgcctacaac gacgtgaatt ccgcgctcgc cgtgatccgc gcgacccgcg
     1921 aggttcgccc ggatgttccg atcctggccc gcaccagcga tgatgcgcgc ctcgaggaac
     1981 tgcgcgccgc cggggcggac gaggtgatcc cggagacgct cgaggcgagt ctcatgatcg
     2041 gctcgcatct gctcgcgacg ctcggcgttc ccatgcgcag aattctccgc caggtaagcg
     2101 acgttcgcgc cgggcgctac agtctcatgc gcaacgtcta tcggcgaagc ggcgccgagg
     2161 cgctcgacat ggatcacgcc ttccgcgggc aactcaacgc ggtgacgctg gtcggaaacg
     2221 cgcacgggat cggacgctcg ctcggcgcac tcgatctcga agcgctcggc gtcggcgtca
     2281 ccgcgcttcg ccgcgacggt gtcgtcggcc gcgagcccgg gccggaaacg gtgctgcgcg
     2341 aaggcgacac cctggtcctc tacgggacgc ccgaagaggc caagcgcgcc gaagatcgcc
     2401 tcctcgcggg ttgaggtaca acggcagttt caggttgccc agtcccgagt cccggtcaaa
     2461 gtcccagtcc cgagcgcgca cgtatacttt tcgtgcttga cactgacgtg aaccatgcgc
     2521 tttcaccgtc tcgtctttct cgacctcgcg accgtcgacc gcggcgatct cgatctctcc
     2581 ccgatcgttt cgcggacacg ggagttcctg ccgtggccgg ccaccgcgcc ggacgaggtt
     2641 gccgggcacg tcgcggccgc cgacgccatc gtgctgaaca aggtacgtct gggcgcagag
     2701 gttttcgagc gtgtacagcc gcgcatcgtc tgtctcgccg cgaccggcac cgacaacgtg
     2761 gatctcgtcg ccgcccgtga ggccgggatc gcggtcgcca acatccgcga ttactgcacc
     2821 gattcggtcg tgcagcacgt cttcgcgctg ctgctcgcgc tcagcacgcg tctcgccgag
     2881 catcgcgagc atctgcaaac cggggcgtgg ccgccgggcg gcgcctacac cctccccgat
     2941 ctgccgatac gcgaactcgg cggcaaaacg ctcgccgtcg tcggcttcgg gaccctcggc
     3001 cgcgcggtcg cc
//
LOCUS       DASUJG010000110         2358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1133173, whole genome shotgun sequence.
ACCESSION   DASUJG010000110 DASUJG010000000
VERSION     DASUJG010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133173"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="VFX38_02185"
     CDS             complement(<1..959)
                     /locus_tag="VFX38_02185"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX5313704.1"
                     /translation="MAAEVTIVGTGLAGPLAALLLAQNGWRVTLVERRPDPRTGEFAG
                     GRSINLALAARGLRALAAAGLAEAVKPLLIPMRGRMTHSREGKTELLPYGQRPDEVIH
                     SVSRQALNRLLVSAAAETGRVEFLFRRRLEDFDPPGGAVILVDDDGERETRKVEALIG
                     ADGAGSALRGALVRRGLLAAREDLLDHGYKELSITPGDGGRHRLEREALHIWPRGGFM
                     LIALPNIDGSFTVTLFLAREGEPGFSSLATPQAVEDFFAREFPDAHALLPQIGEAFFS
                     NPTGALGTVHAEPWHAADKVLLIGDAAHAIVPFHGQGMNAAFE"
     gene            complement(949..2229)
                     /gene="kynU"
                     /locus_tag="VFX38_02190"
     CDS             complement(949..2229)
                     /gene="kynU"
                     /locus_tag="VFX38_02190"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HEX5313705.1"
                     /translation="MKEDWLLLARESDADEASGTFRERFCLPPGPDGNPAAYLCGHSL
                     GLCPKSARELVAEEFEDWAALGVEAHIRARRPWVSYHEELAPALARIVGAREHEVVAM
                     NSLTTNLHLMMASFYRPVRERHAILIEKGAFPSDRHAVVSQLAYHGYSADDALIELAP
                     RAGAATLREKDIEVALEREGGRVALVLWPGVQYLTGQAFDLARIARAAHEAGAVAGFD
                     LAHAVGNLALSLHDDAADFAVWCSYKYLNGGPGAPGGCFVHERHGLEFAGPRFTGWWG
                     HDKRSRFAMGPEFLAIPGAEGWQLSNPPVFALAPLLASLRIFDEAGIAKLRARSLELG
                     GFLADALAVELGDAIEIITPAEPDAHGAQLSLRLRRDREIARRAFDGLAALGVVADWR
                     EPDVIRVAPAPLYCTHEDCARLVNALHDTLRDGG"
BASE COUNT          425 a          797 c          790 g          346 t
ORIGIN      
        1 tcctcgaagg cggcgttcat gccctggccg tgaaagggca cgatcgcatg ggcggcatcg
       61 ccgatcagga gcaccttgtc cgcggcgtgc cagggttcgg cgtgcacggt gccgagcgcg
      121 ccggtgggat tggagaagaa cgcctcgccg atctgcggca ggagcgcgtg cgcgtcggga
      181 aattcgcgcg cgaaaaaatc ctccaccgcc tgcggtgtcg caagagaaga aaaccccggc
      241 tcgccctcgc gcgcgagaaa aagcgtgacg gtgaaactgc cgtcgatgtt cgggagcgcg
      301 atcagcatga agccgccgcg cggccagata tggagcgcct cgcgttcgag ccgatgccgg
      361 ccgccgtcgc ccggcgtgat cgaaagctcc ttgtagccgt gatcgagcag atcctcgcgc
      421 gccgcgagaa gaccgcgcct caccagagcg ccgcgcaaag ccgagcccgc accgtcggcg
      481 ccaatcagcg cctccacctt gcgggtttca cgttctccgt cgtcgtcgac caggatcacg
      541 gctccgccgg gcggatcgaa gtcctcgagc cggcgtcgaa aaagaaactc gacccgcccg
      601 gtctccgccg ccgccgacac cagcaggcgg ttgagcgcct ggcgggagac ggaatgaatg
      661 acttcgtccg ggcgttggcc gtagggcaac agttcggtct ttccctcgcg gctgtgcgtc
      721 atgcgcccgc gcatgggaat cagcagcggc ttcaccgcct ctgcgagacc ggccgcggca
      781 agtgcccgaa gtccgcgcgc ggcaagcgcg agattgatcg agcgtccacc cgcaaattcg
      841 ccggttcgcg gatcggggcg gcgctcgacg agcgtgaccc gccaaccgtt ttgcgcaagc
      901 agcagcgccg cgagcggccc ggcaagcccg gtgccgacga tcgtgacttc agccgccatc
      961 gcgaagcgtg tcgtgaagcg cgttcaccaa tcgggcgcaa tcttcatgcg tgcagtagag
     1021 cggagcgggc gcgacgcgga tcacgtcggg ttcgcgccag tcggcaacga caccgagcgc
     1081 ggcgagtccg tcgaaggccc ggcgcgcaat ttcccgatcc cggcggagac gcagcgacag
     1141 ttgcgcccca tgcgcgtcgg gctcggcggg agtgatgatc tcgatggcgt cgcccagctc
     1201 gaccgcgagc gcatcggcaa gaaagccgcc gagttcgagt gaacgcgcgc gcagcttcgc
     1261 gatgccggct tcgtcgaaga tgcgaagcga cgcgagcagc ggcgcgagtg cgaacaccgg
     1321 cggattcgac aattgccagc cttcggcgcc gggaatcgcg aggaactccg gacccatcgc
     1381 aaaacgcgag cgcttgtcat ggccccacca gccggtgaaa cgcgggccgg cgaattcgag
     1441 tccgtggcgc tcgtgcacga aacagccgcc gggcgcgccg ggcccgccgt tgagatactt
     1501 gtagctgcac cagacggcga aatcagccgc atcgtcgtgg agtgagagcg cgagattgcc
     1561 gacggcatga gcgagatcga agccggcgac cgcacccgcc tcgtgcgcgg cgcgggcgat
     1621 gcgcgcgaga tcgaaggcct gaccggtcag atattgcacg ccgggccaga ggacgagcgc
     1681 gacgcgcccg ccttcgcgtt cgagcgcgac ttcgatgtct ttctcgcgca gcgttgccgc
     1741 accagctcgc ggcgcgagtt cgatcagcgc gtcgtccgcc gaatagccgt gatacgcgag
     1801 ttgtgaaacg acggcgtggc gatcggaggg gaaggcgccc ttctcgatca ggatcgcatg
     1861 acgttcgcgc accgggcggt aaaacgacgc catcatcaga tgcagattgg tcgtaagcga
     1921 attcatcgcg acgacttcgt gttcgcgcgc gccgacgatc cgcgcgagcg ccggcgcgag
     1981 ttcctcgtga tagctcaccc aggggcggcg tgcgcggata tgcgcctcga cgccgagcgc
     2041 cgcccagtcc tcgaattctt ccgccaccag ctcgcgcgcg ctcttcgggc acagcccgag
     2101 cgaatgcccg caaagatacg ccgcgggatt gccgtccgga ccgggcggca gacaaaagcg
     2161 ctcgcgaaac gtccccgacg cttcatccgc atcactctcc cgcgccagca gcagccagtc
     2221 ttccttcatg cccctttact ccgcggtgaa tggtggattc gcaaaattcc cgtgggttgc
     2281 gcccgcgaga aaccgagtcc tttcccttcc gacactcata tcagctccgc cggaacgacg
     2341 agaggacggc tgggcacc
//
LOCUS       DASUJG010000111         3529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_643375, whole genome shotgun sequence.
ACCESSION   DASUJG010000111 DASUJG010000000
VERSION     DASUJG010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643375"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..113)
                     /locus_tag="VFX38_02195"
     CDS             complement(<1..113)
                     /locus_tag="VFX38_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006473810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEX5313706.1"
                     /translation="MTECVFCKMARGEIKPDVVYADDEILAFRDLNPQAPV"
     gene            complement(255..860)
                     /gene="recR"
                     /locus_tag="VFX38_02200"
     CDS             complement(255..860)
                     /gene="recR"
                     /locus_tag="VFX38_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX5313707.1"
                     /translation="MASVLPPALSELVAALRCLPGVGPKSAQRAAFHLIERDREGAER
                     LAHALVHAGRAVTHCRRCRMLAEAELCVICANPNRNERELVVVESPSDVAAIEGATSY
                     AGHYFVLMGRLSPIDGIGPAELGAAELEARLASGVEEVIVATGATVEGEATAHWIGEI
                     AHRHGVRATRIAHGVPVGGELDYVDGSTLARAFSGRRSVED"
     gene            complement(877..1197)
                     /locus_tag="VFX38_02205"
     CDS             complement(877..1197)
                     /locus_tag="VFX38_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009150605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX5313708.1"
                     /translation="MPMDFGAMLREAQNLKSRLGEMQSELAQLECNGEAGGGQVRVRV
                     NGNFEVRRVEIEAQALGDHAMLEDLVAAATNDALARVRKAIQERFAGLAGNLPLPPGL
                     LPGA"
     gene            complement(1201..2757)
                     /gene="dnaX"
                     /locus_tag="VFX38_02210"
     CDS             complement(1201..2757)
                     /gene="dnaX"
                     /locus_tag="VFX38_02210"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX5313709.1"
                     /translation="MAYLALARRYRPRNFEEVVGQQHVVRALANALDGARLHHAYLFA
                     GTRGVGKTTIARILARCLNCEKGVSSHPCGECGACRAIDAGRFLDLIEVDAASRTKVE
                     DTRDLLENVPYAPAEGRFKVYLVDEVHMLSNHSFNALLKTLEEPPEHVKFLFATTDPE
                     KVPATVLSRCLVFTLHRLATAEIEGALARIVEKEGLVAEPAALAAIARAGRGSLRDAL
                     SLLDQALAFGGSGGLTAAEAATMLGMTDRGGIYDLLDRLAADDGRGLLARLAEILGAA
                     SGVDDILDALAETLALVARRQLVAEAPLAADVPAQRVDALAHALTPEEVQLDYDIVLA
                     GKQDLDYAPDPTAALEMTLLRMLAFRPQPDSAGGESRRPATRARSVAAKPGTAPGGKV
                     SGEDWEAILSRLKIGGMLRELAGHCTLAGRDDNIIRLRLDARGRELLTPRLQAGLEAA
                     LADELGAGLKLKIEIGETAAPTPAARRSAREAERQARAEEEIARDPQVAKLRETFGAE
                     LIPDSIRPED"
     gene            complement(2973..3068)
                     /gene="ffs"
                     /locus_tag="VFX38_02215"
     ncRNA           complement(2973..3068)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFX38_02215"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3308..3398)
                     /locus_tag="VFX38_02220"
     tRNA            complement(3308..3398)
                     /locus_tag="VFX38_02220"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3362..3364),aa:Ser,seq:gga)
BASE COUNT          567 a         1204 c         1183 g          575 t
ORIGIN      
        1 tgcaccggcg cctgcggatt gagatcgcga aaggcgagaa tttcgtcgtc cgcgtacacg
       61 acgtcgggct tgatttcgcc tcgcgccatc ttgcagaaca cgcactcggt catgattgct
      121 ccggaacggc cgagaaaata taccgcagcc ttttgtaggc tgggctgagc aaagcgaagc
      181 ccaacgtcgt gcatgcggta atgttgggct tgcgttgggc ttcactacgt tcagcccagc
      241 cgactcgctt catgtcagtc ctcgacgctg cgccgaccgc tgaaggcacg cgcgagcgtg
      301 gagccgtcca cgtaatcgag ttcgccgccg acgggaacgc cgtgggcgat gcgggtcgcg
      361 cgcacgccgt ggcgatgtgc gatttcgccg atccaatgcg ccgtcgcctc gccctcgacc
      421 gtcgcacccg tcgcgacgat cacctcttcc actccgctcg cgagccgcgc ctcgagttcg
      481 gcggcaccga gttcggcggg accgatgcca tcgatcggcg acagccgccc catcagcacg
      541 aaatagtggc cggcgtagga cgtggctccc tcgatcgcgg cgacgtcgct cgggctttcc
      601 acgaccacga gttcgcgctc gttgcgattc gggttggcgc agatcacaca aagttccgct
      661 tcggcgagca tccggcaacg ccggcaatgg gtcacggcgc gcccggcgtg aacgagcgca
      721 tgcgcgagac gttccgcgcc ttcgcggtcg cgctcgatga gatggaaggc cgcgcgttgc
      781 gccgacttcg ggccgactcc ggggaggcag cggagcgcgg cgaccagttc gctcagcgcg
      841 ggcggcagta cggaggccat ctcgacggca gcagtgtcag gcgcccggca acaggccggg
      901 aggaagcgga agattgccgg cgagcccggc gaagcgctcc tggatcgcct tgcgcacgcg
      961 ggcgagtgcg tcgttggtcg cggcggcgac caggtcttcg agcatggcgt gatcgcctag
     1021 cgcttgcgcc tcgatctcca cccggcggac ttcgaaattg ccgttcacac gcacccgcac
     1081 ctgtccgccg cccgcctcgc cgttgcattc gagctgcgcg agttcgctct gcatttctcc
     1141 gagccgcgat ttcagattct gcgcctcgcg cagcatcgcg ccgaaatcca tgggcatgct
     1201 tcaatcctcc ggacggatgc tgtcgggaat gagttcggcg ccgaaggtct cgcgcagctt
     1261 cgcgacctgc gggtccctcg cgatttcttc ctccgcgcgg gcctgccgct cggcctcgcg
     1321 ggccgaacgc cgcgccgccg gagtcggcgc cgcggtttct ccgatctcga tcttgagttt
     1381 cagcccggcg ccgagttcgt ccgcaagcgc cgcttcgagg cccgcctgca atcgcggcgt
     1441 caggagctcc cgcccgcgcg catcgaggcg cagccggatg atattgtcgt cgcggccggc
     1501 gagcgtgcaa tgtccggcaa gctcgcggag catcccgccg atcttgagcc gcgaaagaat
     1561 cgcctcccag tcctctcccg agactttgcc gccgggagcc gtgccgggct tcgcagccac
     1621 actgcgggcc ctcgtcgccg ggcgccggct ttcgccgccc gcggagtccg gctgcgggcg
     1681 aaacgcgagc atgcgaagca gcgtcatctc gagcgccgcc gtcggatcgg gcgcgtagtc
     1741 gagatcctgc ttccccgcca acacgatgtc gtagtcgagc tgcacttctt cgggcgtgag
     1801 cgcgtgggcg agcgcatcca cccgctgggc gggaacgtcg gcggcgagcg gagcctcggc
     1861 cacaagctga cggcgagcga cgagcgcgag cgtctcggca agcgcatcga gaatatcgtc
     1921 caccccgctc gcagcgccaa ggatttccgc gaggcgtgcg agaagtcccc gtccgtcgtc
     1981 ggccgcgagc cgatcgagaa gatcgtagat gccgccgcga tccgtcatgc cgagcatggt
     2041 cgcggcctcg gccgccgtga gcccgccgct gccgccgaag gcgagcgcct gatcgagcag
     2101 cgacagggca tcgcgcaacg atccgcgccc ggcgcgcgcg atcgcggcga gcgccgccgg
     2161 ctcggcgacg agcccttcct tctccacgat gcgcgcgagc gcgccctcga tttccgcggt
     2221 cgcgagtcgg tgaagcgtga acacgaggca gcgcgacagc accgtcgccg gaaccttttc
     2281 cggatcggtc gtcgcgaaca ggaatttcac gtgctccggc ggctcttcga gcgttttcag
     2341 cagcgcgttg aaggaatggt tggaaagcat gtgcacctcg tcgacgagat acaccttgaa
     2401 gcgcccctcg gccggagcgt agggaacgtt ttcgagaaga tcacgggtgt cttccacctt
     2461 ggtgcgcgag gccgcatcca cttcgatcag gtcgagaaag cgaccggcat cgatcgcgcg
     2521 gcaggcaccg cactcgccgc agggatgtga cgaaactccc ttctcgcaat tcaggcagcg
     2581 ggcgagaatg cgcgcgatcg tggttttgcc gactccgcgc gtgccggcga aaagatacgc
     2641 gtggtgcagg cgcgcgccgt cgagcgcgtt ggcgagcgcg cgcacgacat gctgctggcc
     2701 cacgacctcc tcgaagttgc gcgggcgata gcggcgtgcg agcgctagat aagccatgat
     2761 gtcacccaaa cgatgcggcg ttgcgcgacg agaatacggg cgcagggcgc accttccgct
     2821 cgcaacggcc gcgtgttcca tcggtaggaa aacgcggcct tgttcccgcg gccgactccc
     2881 gtcgaagcga attttctcgc gagcgatcga gcacctgaaa aatcccgagc gagctcgttc
     2941 ctaccctgga aggggacaaa aagggtggcg gcctgcaccc gccggccccc ggcacccgaa
     3001 tcggccgctg ccgctgctcc cttccgggcc tgacggggtt cacggcgtat cgtcgcgggg
     3061 agcggatgca gaccaccata aaatttgcgc tgccgaggat tacagtcacg gtcttccggg
     3121 ttgcgaaagt ccggagtccg tacgctcctg cgcccactcg ttcgagaaaa ttgtggcaaa
     3181 gcgtcgcgag cgaagtcgga tcgggcgcgg ccggagcgca ggcaccgcag tgtacggaag
     3241 ggtacatgag gatgccgagc accgcccgag cccgatccgg cgagcacagc agctttgcca
     3301 caatttttgg cggagagggc gggattcgaa cccgcgatgc gcggtgagcg catacacgct
     3361 ttccaggcgt gctccttcga ccgctcggac acctctcccg gtcatgaact tgctcggcgg
     3421 gcccgtcact gtctccggcc gcccgcggca gccgttgccg cccgcgcggc cgcggccgcc
     3481 gtgagcgcgt tcggcggctg cacgagacag gcctcgtcgc gggtcgggc
//
LOCUS       DASUJG010000112         7742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_399586, whole genome shotgun sequence.
ACCESSION   DASUJG010000112 DASUJG010000000
VERSION     DASUJG010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7742
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399586"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..1281)
                     /locus_tag="VFX38_02225"
     CDS             complement(7..1281)
                     /locus_tag="VFX38_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX5313710.1"
                     /translation="MPARNAFYAQSGGVTAVINATAGAVIETAREHRGAIGRLYAGRN
                     GILGALREELIDTGRESRAAIAALRHTPGGAFGSCRYKLKGLDESKAQYERLIAVFRA
                     HDIGYFFYNGGNDSADTAWKVSQLGRRLGYPLACIGIPKTVDNDLAVTDCSPGFGSVA
                     KYVAVSTREAALDVASMYETSTKVFILEVMGRHAGWIAAAAGLAGSGPGEAPHLVLFP
                     ERAFDEADFLRRVDAWVKRYGYCVVVASEGVRDSDGRFLSESGGHDAFGHAQLGGLAP
                     RLAALVKERLGHKYHWAVADYLQRSARHIASQTDLDHALAVGRAAVEFAVAGENAVMP
                     VIERTSDEPYRWRIAKAPLAKIANHEKKLPASFIRRDGYGITAAARRYLVPLIAGEAY
                     PPYAAGMPRYASLRCELVPRKLPPWEDGKAKR"
     gene            1435..2112
                     /locus_tag="VFX38_02230"
     CDS             1435..2112
                     /locus_tag="VFX38_02230"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX5313711.1"
                     /translation="MRIVLLGAPGSGKGTQAKRLSERYGMPQIATGDLLREALASGSE
                     LGRRAKEAMDSGRLVSDDTVFGIIRERLGRADARTGFILDGFPRNIAQAEVLDKLLRE
                     LAQPLDIAIMLQIDQDTLVQRLTGRLTCPSCGRVYNLYTKPPRIEGQCDVDGSELMHR
                     SDDNESTIENRLRVYESHTRPVADYYKNQERLRTVDAEGEIAEVAKRIAQAVRPFVRN
                     GSRKRRA"
     gene            complement(2132..3337)
                     /locus_tag="VFX38_02235"
     CDS             complement(2132..3337)
                     /locus_tag="VFX38_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005003619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX5313712.1"
                     /translation="MMHGALMAFLEFLPRGTVPLPWWGYVLVTAFTVHLTVIGVTIYY
                     HRDQAHRALDLHPALRQFFRFWVWFTSAMVCHQWVAVHRKHHAFVEREGDPHSPQLLG
                     LGHMLGNGARVYQLATMDPATIETYGRGTPNDWLERFYTRFPHLGVTVLLIVEIILFG
                     LPGVAIWGIQMLNMPVLASGLINGVGHHSGYRNFDLPDASHNILPIGILAGGEELHNN
                     HHAFPSSARFSVRRFEFDIGWMYIRVLSALGLARVRRVFPRPHLSERPARIDVDSVRA
                     VVANRMHVLRAYRREVLIPMLRRELAERRRERQFRHMRRLLLKDSTLMDSSKLSQRER
                     LIERYPGLAVAWRYRERLRAIWEERAASNERVRKSLSNWCQDAEASGIESLARFATRL
                     TRYRLAPST"
     gene            3481..4419
                     /locus_tag="VFX38_02240"
     CDS             3481..4419
                     /locus_tag="VFX38_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5313713.1"
                     /translation="MNAETAPLLEVRGLQRRFGRQQAVRGLDFDLRRGEVLALLGPNG
                     AGKTSTLSLLSGNLAPHAGSIRIAGHDLLARPRAAKRALGYLPEHPPLYPELTVAEYL
                     GYCARLHGVGGRAATAAAKSAGERCGLASVAQRLIGQLSKGYRQRVGLAQAIVHRPQL
                     LLLDEPTVGLDPMQLAEVRRLIRDLCADAALIVSTHLLSEAAEVASRVMILHHGRVAY
                     DEQAAKAETQPRFRVELARAPETGRLERIDGIARVELLGAGNFVVTAEPAVDPRESLL
                     RSAVEENWGLLSLTAYRPSLEETFVRLTLTSPETAN"
     gene            4434..5171
                     /locus_tag="VFX38_02245"
     CDS             4434..5171
                     /locus_tag="VFX38_02245"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5313714.1"
                     /translation="MARRELRALFRSPLAWLILAGASFVLAWVFLVFLQQFLEVSPRL
                     PADASAPGVSALVGSPSLLWASLVLVILTPLIAMRLIAEEKRGATLTLLRSAPVSTAA
                     IVVGKFSALIAFLWLVVVIAVAMPLTLALGTALDFGRVASGALGLALLAAAFGAISLF
                     MSTLTHQPLLAALASFGALIFLWIINLAANGAGGTAALVWLAFQPHLAPFLRGEIRTN
                     DVAYFVILAALFLGLSIWKLDAERLGA"
     gene            5174..6535
                     /locus_tag="VFX38_02250"
     CDS             5174..6535
                     /locus_tag="VFX38_02250"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HEX5313715.1"
                     /translation="MRITRSTRWLLRAQSLAFVVLLLVLAGLAAWISNTWTFGVDLSY
                     GRRNSLSTSSRALLAKLDQPVTITAFVRPGSQLAGLEHQLLARYRRADPQVRLRFVNP
                     DTALAELRLLDITTVGELYVEYGGRGEKLDDVSEAGVTNALLRLARGGAERIVFVSGH
                     GEADPQGDRNDDLGRFGAALERQGFRVSTQNLAQNPRIAPGTALVVVAGPASAFLPAE
                     VQSLERWIARGGSFLWLSNPGPLYGLAAISRILGVKPLAGTIVDTNATAAGAPDPTAL
                     VISAYGNSAITRDFSLNTLLPDATGFEPTGRGGWHADIFIASPRLPASWLDRGDESDG
                     EYLYHPGKDVPGPVPIAIALSRPAAAGTGSQRAAVIGDVDFLANSFLGNGGNLNLGLR
                     LFNWLSGEDRYLDVVSLPAPDRRLRLSTVEKGGISLGYLVALPLAFLCLALLVWLRRR
                     RRR"
     gene            6508..7371
                     /locus_tag="VFX38_02255"
     CDS             6508..7371
                     /locus_tag="VFX38_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313716.1"
                     /translation="MAAPQATTLMRARIVVLAIVAGLVAAAAVLYLRQHATAPAGNAI
                     VSVPPAAITRLEARMGGRPALVLVRSAHGWRLRHPVRAPAAASRVALLLAALYEAPAR
                     SYPASAMSAGAIGLAAPALTVKVNGETVEYGGLNPVNLLRYLRHGDRIFLTMDRVLPL
                     LAAGPWQFVDLHPIPRDGDIATVRFSRGGTREGRKFRNAWKNVTATRVTPLATASVAA
                     TAEIRIRLVGRRQPLVFRVLARTPELELARPTLGLVYLFPSRIADRLLPSAPAGTHAR
                     TARSRDDPPRT"
     gene            7328..>7742
                     /locus_tag="VFX38_02260"
     CDS             7328..>7742
                     /locus_tag="VFX38_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX5313717.1"
                     /translation="MPELPEVETTRRGLEPHLVGRRIATLLVRDRRLRRPIAAGLERR
                     VAGAEISALSRRAKYLVITTERGSLLIHLGMSGSLRISDRTTPPGNHDHYDLVIAGGP
                     TVRYRDPRRFGLLVWTTAAPERHPLLANLGPEPLGA"
BASE COUNT         1270 a         2675 c         2479 g         1318 t
ORIGIN      
        1 cgtgccttac ctcttcgcct tgccgtcctc ccacggagga agcttgcgcg ggacgagttc
       61 gcatcggagg ctcgcgtagc gcggcatccc cgctgcgtag ggcggatagg cctcgcccgc
      121 gatcaggggg accagatagc ggcgggccgc ggccgtaatg ccgtagccgt cgcggcgaat
      181 gaagctcgcg ggcagctttt tttcgtgatt cgcgatcttc gcgagcggtg ccttggcgat
      241 ccgccagcga tagggctcgt cggaggtccg ctcgatcacc ggcatcaccg cattctcgcc
      301 ggcgacggca aactcgaccg cggcccggcc cacggcgagt gcatggtcga gatcggtttg
      361 cgaggcgata tgccgcgccg aacgctgcag atagtcggcg accgcccagt gatacttgtg
      421 gccgaggcgc tctttcacca gcgcggcaag gcggggagcg agtcctccga gctgcgcgtg
      481 tccaaaggca tcgtggcctc ccgactcgga caggaaacgg ccatcggagt cgcgcacgcc
      541 ctcggaggcg acgacgacgc agtagccgta tcgcttgacc caagcgtcca cgcgccggag
      601 aaaatccgcc tcgtcgaagg cccgctccgg aaaaagtacg agatgcggag cttcgcccgg
      661 accggagcca gcgagaccgg cggcggccgc gatccagccc gcgtgccggc ccatcacctc
      721 gagaatgaat accttggtcg aggtctcgta catggaggcc acgtccagcg ccgcctcgcg
      781 cgtggaaacg gcgacgtact tcgccaccga tccgaagccc ggcgagcagt cggtcaccgc
      841 gaggtcgttg tccacggttt tgggaattcc gatgcaggcg agcggataac cgaggcggcg
      901 gccgagctgc gaaaccttcc aggcggtgtc cgccgaatcg ttgccgccgt tgtagaaaaa
      961 gtagccgatg tcgtgcgcgc gaaacacggc aatcaggcgc tcgtattgcg ccttcgattc
     1021 gtccagccct ttgagcttgt agcggcagga accgaaggct ccgcccggcg tatgccgcaa
     1081 ggccgcgatc gccgctcgac tttcccggcc ggtgtcgatc aattcttcgc gcagcgcccc
     1141 gaggatcccg ttgcgcccgg cgtagagtct gccgatcgcg ccgcgatgtt cgcgggcggt
     1201 ctcgatcacg gcgcccgcgg tcgcattgat gaccgccgtc acgccgccgg attgtgcgta
     1261 aaaggcgttg cgcgcaggca ttgcgccccc gttcccgaac cgacgcggtc attgtgccac
     1321 agggccgcaa ggggagcgtc ctgaacggca ggcattgacg ccgcgcgcaa gctgggctac
     1381 ctttgccgct cgccggcccc agtgcagccg gcttcgtcca cctcgaggca gctcatgcga
     1441 atcgtacttc tgggcgctcc cggctcgggc aagggcacac aggccaagcg cctgagtgaa
     1501 cgttacggaa tgccgcaaat cgcgaccggc gatttattgc gcgaggcact cgcttcgggc
     1561 tccgaactcg ggcggcgcgc caaggaagcc atggattccg gtcgcctggt gtccgatgac
     1621 accgtcttcg gaatcatccg cgaacggctg ggtcgtgcgg acgcccgcac ggggtttatt
     1681 ctcgacggtt tcccccgcaa catcgcccag gccgaggttc tcgacaagtt gctgcgcgaa
     1741 ctcgcgcaac cgctcgacat cgccatcatg ctgcaaatcg accaggacac cctggtccag
     1801 cggctcaccg gacgtctcac ctgccccagc tgcgggcggg tctacaacct ctacaccaag
     1861 ccgccgcgga tcgagggcca atgcgacgtg gacggaagcg aactcatgca tcgcagcgac
     1921 gacaacgaat cgaccatcga gaatcgcctg cgggtatacg aatcccacac ccgcccggtc
     1981 gccgattact acaagaacca ggaaagactc cgcaccgtcg acgcggaagg agaaatcgcg
     2041 gaggtcgcga aaaggattgc tcaggccgtt cggcccttcg tgcgcaacgg atccaggaag
     2101 cgccgggcgt aacgggcgtt actacgcgcc ctcaggtgga aggcgcgagg cggtagcgcg
     2161 tgagacgggt cgcgaaccgg gcgagactct cgattccgct cgcctcggca tcctggcacc
     2221 agttgctgag gcttttgcgc acgcgctcgt tcgaggcggc acgctcctcc cagatcgcac
     2281 gcagccgctc gcgataccgc caggcgacgg cgagtccggg gtagcgttcg atcagccgct
     2341 cgcgctggct gagcttgctc gaatccatca gcgtcgagtc tttgagcagc aggcggcgca
     2401 tgtgccggaa ctggcgttcg cgccgacgct cggcgagttc gcgccgcagc atcgggatca
     2461 gcacctcgcg gcggtaggcc cgaagcacat gcatgcgatt cgcgaccacg gcacgaaccg
     2521 agtcgacatc gatccgcgcc ggccgctcgg agagatgcgg gcgcgggaaa acccggcgca
     2581 cccgggcgag tccgagcgcg ctcagcacgc gaatgtacat ccagccgatg tcgaactcga
     2641 agcgacgcac cgagaagcgc gccgaactcg ggaaggcatg atgattgttg tgcagttcct
     2701 cgcctccggc gaggatgccg atcggcagga tattgtgcga ggcgtcgggc agatcgaaat
     2761 tgcggtagcc gctgtgatgg ccgaccccat tgatgagccc cgaggcgagc accggcatat
     2821 tgagcatctg gataccccag atcgcgacac ccggcagacc gaagagaatg atttctacga
     2881 tcaggagcac ggtcacgccg agatgcggga aacgggtgta gaagcgctcc agccaatcat
     2941 tgggcgtgcc gcgaccgtag gtttcgatcg tcgcgggatc catggtcgca agctgataaa
     3001 ctcgcgcgcc gttgccgagc atgtgcccga gaccgagcaa ctggggactg tgcggatcgc
     3061 cttcgcgctc gacaaaggcg tggtgcttgc ggtggacagc cacccactga tggcatacca
     3121 tcgcgctcgt gaaccagacc cagaagcgga aaaactgccg caaagccgga tgcaggtcga
     3181 gcgcgcgatg cgcctgatcg cgatggtagt agatcgttac gccgattacc gtgagatgca
     3241 cggtaaatgc cgtgaccagc acatatcccc accacggcag aggaaccgtg ccgcggggga
     3301 gaaattcgag aaacgccatc aacgcgccat gcatcatgca ttcagtctaa cccagcgggt
     3361 gccggcggcg gaaccccggt ccccgagcct cgtcgcaacg gcccgaccgg ccgctgcggc
     3421 gcagggcagg cgccgcacgg ccgcgccgcg gatcctgcga ctttccggcg caatcgcgcg
     3481 atgaacgccg agaccgctcc gctgctcgaa gtgcgtgggc tccaacgtcg ttttggaaga
     3541 cagcaagcgg ttcgcggcct tgatttcgac ttgcggcggg gagaggttct ggcgctgctc
     3601 ggcccgaacg gcgccggcaa gacgtccacg ctttccttgc tttccgggaa ccttgccccg
     3661 cacgcaggca gcattcgcat cgccgggcat gatcttctcg caagaccgcg agccgcaaaa
     3721 cgagcgctgg gttaccttcc cgaacacccg ccgctctatc cggaactcac cgtggcggaa
     3781 tatctcggct attgtgcccg tctgcacggc gttggcggtc gggccgccac ggccgcggcg
     3841 aagtccgccg gcgagcgttg cggtctcgca tcggtcgcgc aacgcctgat cgggcagctc
     3901 tcgaaaggct atcgtcagcg ggtcggcctc gcccaggcaa tcgtgcatcg cccgcaactg
     3961 ctgctgctgg acgagccgac cgtgggactc gatccgatgc agcttgccga agtgcgccgg
     4021 cttatccgcg atctctgcgc cgatgcggcg ctcatcgtct ccactcatct gctctcggag
     4081 gcggccgaag tcgcgagccg ggtgatgatt ctccatcacg gccgggtcgc ctacgacgag
     4141 caagccgcaa aggccgaaac gcagccgcga ttccgcgtgg agttggcccg cgcgcccgag
     4201 accgggcgat tggaacgcat cgacggaatc gcgcgggtcg aacttctcgg cgccggcaat
     4261 ttcgtagtca ccgccgagcc cgccgtcgat ccgcgcgaat cgctgcttcg cagcgccgtg
     4321 gaagaaaact ggggtctgct gagcctcacg gcctatcgac cgtcgctgga agaaactttt
     4381 gtccggctga cgttgacgag tccggagacg gcaaactgaa cgccaccgcc gtcatcgccc
     4441 ggcgcgagct gcgtgcgcta tttcgcagcc cgctcgcctg gctcatcctc gccggcgcga
     4501 gcttcgttct cgcttgggtt ttcctcgtgt ttcttcagca gttccttgaa gtgtctccgc
     4561 gactcccggc cgacgcgagc gcccccggag tgagcgccct cgtcggctcg ccgtcgctgc
     4621 tctgggcgag cctggtgctc gtgattctca ccccgctcat cgcgatgcgg ctcatcgccg
     4681 aggaaaagcg cggcgcaacg cttacgctgc tgcgttcggc gccggtatcg accgccgcga
     4741 tcgtggtcgg aaaattttcc gctctgatcg ctttcctgtg gttggtcgtc gtcatcgccg
     4801 tcgcgatgcc cctgacgctc gcgctcggaa cggcgctgga cttcggacgc gttgcgagcg
     4861 gcgcgctcgg gctcgcactt ctcgccgcgg ccttcggcgc gatcagtctg ttcatgtcga
     4921 ccctcaccca ccagccgctc ctcgccgcgc tcgcaagctt cggcgcgttg atttttctct
     4981 ggatcatcaa cctcgcggcg aacggcgcag gaggaaccgc cgcgctcgtg tggctcgcgt
     5041 tccaaccgca cctcgcgccg tttctccgcg gcgagattcg aacgaacgac gttgcgtatt
     5101 tcgtcatcct ggcggcgctt tttctggggt tgtccatctg gaaactcgac gcggagcgcc
     5161 tcggcgccta ggcatgcgaa tcactcgatc cacgcgctgg ctgctccggg cgcaaagcct
     5221 ggcattcgtc gtcctgcttc tggttctcgc ggggctcgcc gcctggatct cgaacacctg
     5281 gacatttggc gtcgaccttt cctacggccg gcgcaacagc ctgagcacaa gttcccgggc
     5341 gctccttgcg aaactcgatc agcccgtgac gatcaccgcc ttcgtgcgcc cgggcagcca
     5401 actcgccggg ctcgagcacc agcttctcgc acgttaccgg cgcgcggacc cacaagttcg
     5461 cctgcgtttc gtcaatccgg atacggcgct cgccgaactg cgcctcctcg acatcacgac
     5521 cgtcggagaa ctgtacgtcg agtacggcgg acgcggcgag aagctcgacg atgtgagcga
     5581 agccggcgtg accaacgccc tgttgcggct cgcccgcgga ggagccgaac gcatcgtctt
     5641 cgtcagcggg cacggcgaag cggatcccca gggcgatcgc aacgacgatc tcgggcgctt
     5701 cggcgcagcg ctggagcggc agggctttcg agtctccaca caaaatcttg cacagaaccc
     5761 gcgcatcgcg ccgggcaccg ctctggtcgt cgtcgccggt cccgcgagcg cgtttctccc
     5821 ggccgaagtg caatcgctcg aacgctggat cgcgcgtggt ggaagttttc tctggctcag
     5881 caatcccggt cctctgtacg ggctcgcggc gatttcccgg atcctcggcg tgaaaccgct
     5941 cgccggcacg atcgtggaca ccaacgccac cgctgccgga gcgcccgatc cgaccgcgct
     6001 ggtaatttcc gcctacggca actccgcgat cacccgggat ttttcgctga ataccctgct
     6061 tcccgatgcg accggattcg aaccgaccgg ccgcggcggc tggcacgcgg acattttcat
     6121 cgcgagcccg cgtctgccgg caagctggct cgatcgcggc gacgagagcg acggggagta
     6181 cctctaccat cccggcaagg acgtgccggg gccggtcccc atcgccattg ccctgagccg
     6241 gccggcagcc gccggaaccg gctcgcaacg tgccgccgtc atcggagacg tcgacttcct
     6301 cgccaacagc tttctcggca acggcgggaa tctcaatctg ggcctgcggc tcttcaactg
     6361 gctgagtggc gaggaccgct acctcgatgt tgtcagcctg ccggcgccgg atcggaggct
     6421 ccgcctctcg accgtcgaga aaggcggcat cagtctcggt tacctcgtcg cgttgccgct
     6481 cgcattcttg tgcctggcgc tgctcgtgtg gctgcgccgc aggcgacgac gctgatgcgc
     6541 gcccgcatcg tcgtcctcgc gatcgtcgcc ggcctggtcg cggcggcggc ggtcttgtac
     6601 ctccggcagc acgcgaccgc gccggcggga aacgccatcg tgtcggttcc cccggcggcg
     6661 attacgcggc tcgaagcgcg aatgggcggc cgccccgccc ttgttctggt gcgcagcgcg
     6721 cacggctggc gcctgcgcca tccggtacgg gcgcccgccg cagcgagccg ggtcgccctg
     6781 ctgctggcgg ctctctacga ggctccggca cgaagctatc cggcatcggc gatgtcggcc
     6841 ggcgccatcg gcctcgcagc tcccgcgcta accgtgaagg tcaatggcga gacagtcgag
     6901 tacggcggtc tcaatcctgt caatcttctt cgctatctgc gccacggcga tcggattttc
     6961 ctgaccatgg accgcgtgct gccgctcctt gcggcgggcc cctggcagtt cgtcgacttg
     7021 cacccgattc cgcgggacgg tgacatcgcg accgtacgtt tttctcgcgg cgggacgcgc
     7081 gaaggacgca aatttcgtaa cgcctggaag aacgtcaccg ccacgcgcgt cacgccgctc
     7141 gcgaccgcct ccgtggcggc caccgccgaa atccgcattc gtctggtcgg ccgccggcag
     7201 cccctcgtat tccgggttct cgcccggact cccgaactgg aacttgcccg cccgacgctc
     7261 ggcctcgtct atctcttccc ctcgcggatc gcggatcggc tgcttccctc cgcccccgcc
     7321 ggcacacatg cccgaactgc ccgaagtcga gacgacccgc cgcggacttg agccgcatct
     7381 agtcggccgg cgcatcgcca cgcttctcgt gcgcgatcgg cgcctgcgcc ggccgattgc
     7441 ggcaggtctg gaacggcggg tcgcgggcgc cgaaatctcc gcactttcgc ggcgcgcaaa
     7501 gtacctcgtg atcacgacgg aacgcgggag cctgctcatc catctgggga tgtcgggatc
     7561 gctgcggatc agcgatcgga cgacgcctcc gggaaatcac gatcattacg acctggtgat
     7621 cgcgggcggc ccgaccgtcc gctatcgcga cccgcgccga ttcggcctgc tggtctggac
     7681 gaccgccgcg cccgagcgcc atccgctact cgccaatctg ggcccggaac ccctcggcgc
     7741 cg
//
LOCUS       DASUJG010000113         2147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1500369, whole genome shotgun sequence.
ACCESSION   DASUJG010000113 DASUJG010000000
VERSION     DASUJG010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500369"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VFX38_02265"
     CDS             <1..313
                     /locus_tag="VFX38_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005333086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HEX5313718.1"
                     /translation="VLPIADRHAEYAASVRDRLAAAGVRVELDGRQEKIGYKIREAQL
                     QKIPYMLVTGDREAAEDTVSVRSRSGGDLGARSVDDFIGDAQAEIAAKRERAKGLAAA
                     S"
     gene            406..978
                     /gene="infC"
                     /locus_tag="VFX38_02270"
     CDS             406..978
                     /gene="infC"
                     /locus_tag="VFX38_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011368470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX5313719.1"
                     /translation="MAFDRSPRRDDRTRVNERIRVREVRVIDEDGTQLGIMAPPQALS
                     IARQKGLDLVEIAATANPPVCRIMDFGRYQYQEQKRARSAKKHQKIIEVKEIKFRPKV
                     DEHDYQFKKKHIERFLSDGDKVKATIFFRGREMAHPEIGRRILERLIEELNDVAVAET
                     APRQEGNQMHTILSQKAGAKRPPAKPTRAE"
     gene            994..1194
                     /gene="rpmI"
                     /locus_tag="VFX38_02275"
     CDS             994..1194
                     /gene="rpmI"
                     /locus_tag="VFX38_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX5313720.1"
                     /translation="MPKIKLKSHRGAAKRFKKTASGKFKRGNAFKAHILTSKERSRKR
                     KLKGTTMVSAQDTAKLRRMLPY"
     gene            1219..1596
                     /gene="rplT"
                     /locus_tag="VFX38_02280"
     CDS             1219..1596
                     /gene="rplT"
                     /locus_tag="VFX38_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX5313721.1"
                     /translation="MPRVKRGNVRRAKRKKLLSRAKGFYQTKSKLYRSAKESVDTALK
                     YAFVGRRNKKRDFRRVWVMRINAAARENGLTYGQLISGLKAAGSTLDRKSLAELAVSS
                     PASFARLAKIAADARPKQAAATK"
     gene            1835..>2147
                     /locus_tag="VFX38_02285"
     CDS             1835..>2147
                     /locus_tag="VFX38_02285"
                     /inference="COORDINATES: protein motif:HMM:NF014909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX5313722.1"
                     /translation="MDIADLRRRLELDIASIKTEAELRALRDRYLARKNGLISNLLKE
                     VASAPADQRPALGKEANLLKKHVEDAIDGKAAVLGKPHTGTSTDVDVTLPGREPLVGH
                     LH"
BASE COUNT          498 a          653 c          719 g          277 t
ORIGIN      
        1 cgtgttgccg atcgcggatc gccacgcgga atacgccgca agcgtgcgcg accggctcgc
       61 cgcggccggc gtccgggtcg agctggacgg gcggcaggaa aaaattggat ataagatccg
      121 cgaggcccaa ttgcagaaga ttccgtacat gctcgtgacc ggcgatcggg aagccgccga
      181 ggacacggtg tcggtgcgga gccgcagcgg cggcgacctg ggcgcgcgca gcgtggacga
      241 cttcatcggc gacgcgcagg cggagatcgc cgccaaacgc gagcgggcga agggactcgc
      301 ggcggcgagc tgacggatcg caggagatca gaagatcaga gggaaatttt cttttcctcc
      361 agacctcctg gtctcctgca atatggtttg acgaggaggt agtttatcgc tttcgacaga
      421 agcccccggc gcgatgaccg gacgcgcgtg aacgagcgaa ttcgggttcg cgaagtccgc
      481 gtcatcgatg aggacggaac gcagctcggc atcatggccc caccgcaggc gctctccatc
      541 gcgcggcaga aggggctgga cctcgtggaa atcgcggcga cggcgaaccc gcccgtctgc
      601 cggatcatgg acttcgggcg ctaccagtac caggagcaga agcgcgcgcg gtccgcaaag
      661 aagcatcaga agatcatcga ggtcaaggaa atcaagttcc ggccgaaggt cgatgagcac
      721 gactaccagt tcaagaagaa gcacatcgag cgtttcctct cggacggcga caaggtgaag
      781 gcgacgatct tcttccgcgg gcgcgaaatg gcgcacccgg agatcgggcg gcgcattctc
      841 gagcgcctga tcgaggaatt gaacgatgtc gcggtcgcgg agaccgcgcc gcggcaggaa
      901 ggcaaccaga tgcacacgat cctgtcgcaa aaggcgggcg ccaaacggcc gccggccaaa
      961 ccgaccaggg cggaatgagc gagtaggacc gatatgccga aaataaagct gaagagtcac
     1021 cgcggcgcgg cgaagcgatt caagaaaacc gcgagcggca agttcaagcg cggaaatgcg
     1081 ttcaaggcgc acatcctgac gagcaaggaa cgcagccgca aacgcaaact gaaggggacg
     1141 acgatggtgt cggcccagga tacggcgaag ctgcgccgca tgctcccgta ctagtttcga
     1201 gaaagcagag acacgcccat gcctcgagtc aaacgtggaa acgtccggag agccaagcgc
     1261 aagaagctgc tcagccgcgc gaaaggcttc taccagacga agagcaagtt gtatcggtcg
     1321 gcgaaagagt cggtcgatac ggcgctcaaa tacgccttcg tcgggcggcg caacaagaag
     1381 cgcgacttcc gccgcgtctg ggtgatgcgc atcaacgccg ccgcccgcga aaacgggctg
     1441 acgtacgggc agttgatctc cggcctgaaa gcggccggca gcacgctcga tcgcaagagc
     1501 ctcgcggaac tcgccgtctc gtcgcccgcc tcgtttgcgc gcctcgcgaa aatcgcagcc
     1561 gacgcccggc cgaagcaggc cgccgcaacg aaataggcca cggaagacac ggaagacacg
     1621 gaaaacgaag gggccacgga gcaggcggtc aggaccggcc ctgatcgcat gatctgctgg
     1681 ccccttcgac tatagactga caaaaacctg ttcatgacgc cgtcgccgcg cgagtgagcg
     1741 cagcgaacga gccacgcggc cggagcgagc cggcgaagcg gcgagcgagg acgcgtgcag
     1801 gggagtccga ggggcgacgc ccctcggagt gagaatggac atcgccgacc tgcgccgcag
     1861 actcgagctg gacatcgctt cgatcaagac cgaggcggaa ctccgtgcgc tccgggaccg
     1921 gtatctcgca cgaaaaaatg ggttgatctc gaacctgctc aaagaggtgg cctctgcgcc
     1981 ggcagaccag cgcccggcgc tcggaaaaga agcaaacctc ctgaagaagc acgtcgaaga
     2041 cgcgatcgac ggcaaggccg cagtcctggg caaaccccac acgggcacga gcacggacgt
     2101 cgatgtgacg ctgcccggac gcgagccgct cgtcggccac ctgcatc
//
LOCUS       DASUJG010000114         3330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_643488, whole genome shotgun sequence.
ACCESSION   DASUJG010000114 DASUJG010000000
VERSION     DASUJG010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643488"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..1193
                     /gene="alr"
                     /locus_tag="VFX38_02290"
                     /pseudo
     CDS             171..1193
                     /gene="alr"
                     /locus_tag="VFX38_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010215915.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
     gene            complement(1078..1839)
                     /gene="nth"
                     /locus_tag="VFX38_02295"
     CDS             complement(1078..1839)
                     /gene="nth"
                     /locus_tag="VFX38_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010410179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX5313723.1"
                     /translation="MTRDQVFEFFRRLAEANPSPTTELESTNPYQLLVAVVLSAQATD
                     AGVNIATKPLFERVKTPEQMVDFGEERLRETIKTIGLFNTKAKNVIALSRALIDNHGG
                     EVPRTREALQALPGVGRKTANVVLNTAFGEETFAVDTHVFRVCNRTGLAPGKTVDTVE
                     AKLEKIVPQPFRRDAHHWLILHGRYICTARTPECWRCPVIDLCRYQPKTPAPGTKPTA
                     ARSGSRGSSKARTAKGRIAPKPTASRSRSRPTRLP"
     gene            complement(1836..2546)
                     /gene="dapB"
                     /locus_tag="VFX38_02300"
     CDS             complement(1836..2546)
                     /gene="dapB"
                     /locus_tag="VFX38_02300"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX5313724.1"
                     /translation="MRTDDQPIRISLFAPEGRMGKAIAAAVADDPGFAIDQDHGDVLV
                     DFSAPAALQASLDRAVSAGVPILVGTTGLDELADRRIAEAAKDVPVLRAANTALGIAL
                     LAELVARAAEVLGPDWDIEIAETHHRMKADAPSGTALILGHAAARGRGVDFESEVGRA
                     GTGLSRKAGAIGFASLRGGTVAGEHDVRFLGPKEQIVLSHRAENRMIFAKGALAAARF
                     LVGKPAGLYSMHDVIRAK"
     gene            complement(2557..3300)
                     /locus_tag="VFX38_02305"
     CDS             complement(2557..3300)
                     /locus_tag="VFX38_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX5313725.1"
                     /translation="MSEGKIRIGIGGWTFPPWRGSFYPADLPQKRELEYASRQLGVIE
                     INATFYGRQKPKSWEAWEQTVPDDFQFAIKGSRFCVTRSRLCEGAEGIGNFFAQGFAA
                     LGPKLGPILWQFAPRRHFDRDDIAGFIDLLPKELDGIPLRHAIEPRHESFRDEQFFDL
                     CRARNIAIVFEDSDQFPTIEADTADFAYARLQRMSENVPTGYDDEALDGFAERARAWR
                     KRGDAYLFLINGAKVRAPAAALALQERLR"
BASE COUNT          558 a         1064 c         1122 g          586 t
ORIGIN      
        1 gaggcggtgg aactggtggt cgaagcgttc gacgggagga attgggaggc ggccgcggct
       61 tcgatgaagt aggccgtcat tgcgagcaaa gcgaagcaat ccagctttgc gcggatctag
      121 attgcttcgt cgcttgcgct cctcgcaatg acggccctaa cgccctcccc atgcatcgcc
      181 cgctgcgcct gacgatcgac agagccgcca ttcaggcgaa ttggcgctgg ctccaggacc
      241 gggccggcgt gccggccgga gcggcggtca aggccgatgg gtacgggctc ggcgcgcgcg
      301 aggcgaccga gacgctcatc gacgccggtt gccgagactt cttcgtttcg acctgggccg
      361 aggcggaagc gctgggagag gtgcccgaag agataagcgt ggtcgtcctg cacggtgtgg
      421 gacctgacga cgaagacacg gcagttcgac tcgctgcgcg gccatgcctc aacacgcttg
      481 aacaggtcgc gcggtggaag cggatcacgc cggaccgtcc gtgcgacgcg atgatcgata
      541 ccggcatgaa ccggctcgga ctgcgcgcaa ccgaagtcga tgcgctcgaa ggcatgacca
      601 tcgacacgct gcatagccat cttgcctgcg ctgacgaaga cgtagcactg aacaaaatgc
      661 agctagaacg ctttcgagcc gttgcttcag agattcccgc gcggcgctac gctttcgcga
      721 acagcgccgg aatttgtctc ggccgcgact atagcttcaa cctcgtccgt cccggcctct
      781 cgctctacgg cggcgttccc cgtgacgaag ccgagggcaa catccgccag gttgcgcggg
      841 tggaggcgca aatcgtccag cgccggacga tccaggcggg cgagacctgc ggttatggcg
      901 cgaccttcac cgcgcaggag gacacggaag ccgcgatcct caacatcggc tatgcggacg
      961 gatatctgcg cggcttttcc tcacgaggct cggcattcgc gggtgagttc gcgcttccgg
     1021 tcctggggcg cgtgtcgatg gatttgatcg cggtcggctg caacgctgcg cccgagctca
     1081 gggaaggcga gtgggtcgag atcgactacg acttgccgtc ggcttcggcg caatccggcc
     1141 tttcgcagta cgagcttttg acgaccctcg gctcccgctt cgagcggcgg tgggtttagt
     1201 ccccggcgcc ggcgttttcg gctgatagcg gcacaaatcg atcaccgggc agcgccagca
     1261 ttcgggcgtt ctcgccgtgc agatgtagcg gccgtgcagg atcagccaat ggtgcgcgtc
     1321 gcggcggaag ggctggggca cgatcttctc gagcttggct tcgaccgtgt cgaccgtctt
     1381 gccgggcgcg aggccggtgc ggttgcagac tcggaagacg tgcgtgtcga ccgcgaacgt
     1441 ctcttcgccg aacgcggtgt tgagcacgac gttcgcggtc ttgcgaccga cgcccggcaa
     1501 ggcctgaagc gcttctcgcg tgcgcggcac ctccccgccg tgattgtcga tcagcgcccg
     1561 gctgagcgcg atgacattct tcgccttggt gttgaacagg ccgatggtct tgatcgtctc
     1621 gcgcagccgc tcctcgccga aatcgaccat ctgctccggc gtcttcaccc gttcgaacag
     1681 cggcttcgtt gcgatgttca cgccggcgtc ggtcgcctgc gccgacagca ccaccgccac
     1741 gagcagctga tagggattgg tggattcgag ctcggtcgtg ggcgaggggt tcgcctccgc
     1801 gagccggcgg aagaattcga agacctggtc gcgcgtcact tggcgcggat cacgtcgtgc
     1861 atcgaataaa ggccggccgg cttgccgacg aggaagcgcg ccgccgccag cgcgcccttg
     1921 gcgaagatca tccggttctc ggcgcggtgc gacaggacga tctgttcctt ggggccaagg
     1981 aagcggacgt catgctcgcc ggccacggtc cctccccgca gcgaggcgaa gccgatcgca
     2041 cccgccttgc gcgaaaggcc cgtcccggcg cgcccgacct cgctctcgaa atccacgccg
     2101 cgcccgcgcg cggccgcgtg tcccaggatc agcgccgttc ccgatggcgc atcggccttc
     2161 atccggtgat gcgtctcggc gatttcgatg tcccagtcgg ggcccagcac ttccgccgcc
     2221 cgcgcgacca gctcggcgag caaggcgatc ccgagcgcgg tattggcggc acgaaggacg
     2281 ggaacgtcct tggcggcttc ggcgattcgt cgatccgcca gctcgtcgag gccggtggtg
     2341 ccgacgagga tcgggacgcc cgccgatacc gcccgatcga ggctcgcctg aagggcggcg
     2401 ggagcggaaa aatcgaccag cacgtcgcca tggtcctgat cgatcgcgaa gccgggatcg
     2461 tcggccaccg ccgcggcgat ggccttgccc atgcgcccct ccggcgcgaa caggctgatg
     2521 cggatcggct ggtcgtcggt gcgcatgcga cggtgcttag cgaagccgtt cctgcagagc
     2581 cagcgccgca gccggcgctc tcactttcgc gccgttgatg aggaacagat acgcatcgcc
     2641 gcgcttgcgc caggcgcgcg ctcgttcggc aaacccgtcg agcgcttcgt cgtcataacc
     2701 tgttggcacg ttctcgctca tccgctgaag ccgggcataa gcgaagtcgg cggtatcggc
     2761 ttcgatcgtg ggaaactgat cggaatcctc gaaaacgatc gcgatgttcc gggcacggca
     2821 gaggtcgaag aattgctcgt cgcgaaagct ttcgtggcgc ggctcgatcg cgtggcggag
     2881 cggaatgccg tcgagttcct tgggcagcag gtcgatgaag cctgcgatat cgtcgcggtc
     2941 gaaatggcgg cgcggcgcga attgccacaa gatcgggccg agcttggggc cgagcgcggc
     3001 gaagccctga gcgaagaaat tgccgatgcc ttccgcgcct tcgcagaggc gcgagcgagt
     3061 aacgcaaaag cgcgaaccct taatcgcgaa ttggaaatca tcgggaactg tctgctccca
     3121 cgcctcccag cttttcggtt tctggcggcc gtaaaaggtc gcgttgatct cgataacgcc
     3181 tagttggcgc gatgcatatt cgagctcgcg cttttgcggc agatcggccg gataaaaaga
     3241 gccgcgccac ggcggaaagg tccagccgcc gatcccgatg cggattttgc cttcgctcat
     3301 cgcgcggccg ctctatgctc gcggcatgga
//
LOCUS       DASUJG010000115         4125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1622446, whole genome shotgun sequence.
ACCESSION   DASUJG010000115 DASUJG010000000
VERSION     DASUJG010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1622446"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VFX38_02310"
     CDS             <1..396
                     /locus_tag="VFX38_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HEX5313726.1"
                     /translation="DGAYLWRASRGRHAGVKTFVMNSHVVAGVGNIYANEALFRAGID
                     PRRAAGRIAAVRYEQLAAAIRAVLAEAIAQGGTTLRDFVAAEGRPGYFRPRLAVYDRA
                     GEPCPHCRAPLRREVTGQRASYFCPRCQR"
     gene            596..1165
                     /locus_tag="VFX38_02315"
     CDS             596..1165
                     /locus_tag="VFX38_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313727.1"
                     /translation="MNDRVASSKGLGFGVFAIASWLMSMGGAGFMPQAYGGNPSHLVL
                     SVAMIGLLIAAIAAFLRHETWYAFFFMLWSAVSWGLGGGHMGAAAGLAPLAPLGWFWL
                     AIAFVNLYLWLAAMRAELGPAVSLTVLLVSLTMIGLGLQGVLGIGVAGRIGSYLGLAT
                     ALVAFYVSAEAIMNRGGRGARASDGPPVI"
     gene            complement(1233..2699)
                     /gene="tldD"
                     /locus_tag="VFX38_02320"
     CDS             complement(1233..2699)
                     /gene="tldD"
                     /locus_tag="VFX38_02320"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEX5313728.1"
                     /translation="MVNPTTPAAATNLALAQSELLAPGALDERALERLLGRLMARRVD
                     YADLYFQLLREEHWMLEDGIVREGASSVERGVGVRAIAGERSGFAYSDELAFPALAAA
                     CDSARAIATRGGSDRTAGWSESAPARLYRPIDPIATLAAGEKVALLEALDREARRADP
                     RITRVTASLGAVHEVILIAGSDGTLAADVRPLVRVNVSVIAEDHGRREQGHAGGGGRF
                     GYAWFREGERALGFAREAARQALVALEAVAAPAGTMPVVLGPGWPGVLVHEAVGHGLE
                     GDFIRKGTSAFTGRTGERVASALCTVVDDGTLAERRGSLAVDDEGTPTRRNVLIENGV
                     LKGFLYDRLNAQLAGVASTGSGRRESYAHRPLPRMTNTYLMPGPHVPEEIIASVDRGL
                     YAVNFGGGQVDITSGKFVFSASEAYLIEKGRVTRPVRGATLAGSGPEVLNQVSMVGND
                     LALDEGIGVCGKDGQSVPVGVGLPTVKLDRLTVGGTGT"
     gene            complement(2819..3658)
                     /locus_tag="VFX38_02325"
     CDS             complement(2819..3658)
                     /locus_tag="VFX38_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX5313729.1"
                     /translation="MTGKSAAVLQMNSGADVASNLRRARRLLERAAEAGATLAVLPEN
                     FACMPATAADRRRAAEEDGEGPVQDFLGAMAADCRLWIVGGTLPLRIAGETKFHAAVL
                     VYDANGRRVARYDKFHLFDVAVPGRAERYRESRHFVAGRSLVAIETPLGRLGLAVCYD
                     LRFPELFRVLALKHDCQLFAVPSAFTATTGSAHWDSLLRARALENQCFLLAAAQTGRH
                     PGGRETYGRSRILGPWGETLATLGRRSGVAQAPIDLSGQARLRRGFPVLEQARRRELE
                     PGL"
     gene            complement(3655..>4125)
                     /locus_tag="VFX38_02330"
     CDS             complement(3655..>4125)
                     /locus_tag="VFX38_02330"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEX5313730.1"
                     /translation="LVFNFHNLFGKGFPFSRIYGDYVIDRGIASTKGLRIESSVANIK
                     LTGPVNLAAGPMAQRAEISPNYFGSLPVIAAIVGGLGIGAVVYAITKLFGSPLAALTK
                     LHYTISGPIRDPVVKPLGNAPPLKPQPHVQSPPAAASGAAPARGAVPMLRSLPR"
BASE COUNT          652 a         1432 c         1374 g          667 t
ORIGIN      
        1 gacggagcgt acctgtggcg ggccagccgt ggccggcacg ccggcgtcaa aaccttcgtg
       61 atgaactccc acgtcgttgc gggagtcggc aacatctacg ccaacgaggc cctgttccgg
      121 gccggaatcg atccgcggcg ggccgcgggg cgaatcgctg ccgtacgtta cgagcagctc
      181 gcggccgcga ttcgcgctgt cctcgccgaa gccatcgcgc agggtgggac gaccctgcgc
      241 gacttcgtgg ccgcggaggg ccggcccggt tatttccgcc cgcgcctcgc cgtttacgat
      301 cgcgccggag aaccctgtcc gcactgccgg gcgccgttgc gccgcgaagt cacagggcag
      361 cgggcaagct atttctgtcc tcgctgccag cgctgagccc ggcaccgcga accatcgata
      421 cagggcggac cgagcccgcg gacgaccgcg atttgttcgg aatccgtgac ggaaccgtgc
      481 aaaaggaacg cttttgatct aaagtgaaaa ggcttggatt cactcttggc gcctcggcgg
      541 cccggatcga acggcagccg tggcgcctct tctcaccctt gcaaggaggt tcgagatgaa
      601 cgacagagtt gcgagttcca aggggctcgg cttcggcgtc ttcgccatcg ccagttggtt
      661 gatgtcgatg ggcggggccg gcttcatgcc gcaggcctac ggcggaaacc catcgcacct
      721 ggtcctctcc gtggccatga tcgggctgct gatcgcggcc atcgccgcct tcctccggca
      781 cgaaacctgg tatgcgttct tcttcatgct ttggtcggcc gtgagctggg ggctcggcgg
      841 agggcatatg ggggccgcgg cgggtctcgc gccgctggcg ccgctcggct ggttctggct
      901 cgcgatcgca tttgtcaatc tctatctgtg gctcgcggcg atgcgtgcgg aactcggtcc
      961 ggccgtgtcg ctcacggtct tgctcgtgtc gctcacgatg atcggcctcg ggctgcaggg
     1021 cgtcctcgga atcggcgtcg ccgggcgcat cggcagctat ctcggcctgg ccacggcgct
     1081 cgtcgccttc tacgtctcgg ccgaagccat catgaaccgc ggcggccgcg gcgcgcgggc
     1141 aagcgacggc cctcccgtca tctagtgcct ctcttgcgac agggagcggg gcgcccgaca
     1201 gcgtctcgct cccgaacggg agccgtatcg actcaagtgc cggtgccgcc gaccgtgagc
     1261 cgatcgagtt tcacggtagg gagcccaact cccaccggca cgctctggcc gtccttgccg
     1321 cagacgccga tgccctcgtc gagggcgagg tcgttgccca ccatcgagac ctgattcagc
     1381 acctcgggcc cgctgcccgc gagggtcgca ccgcgcaccg gccgggtcac tcgacccttc
     1441 tcgatcagat aggcctcgct tgccgaaaag acgaacttcc ccgaggtaat gtccacctga
     1501 ccgccgccga agttgaccgc gtacaggccg cgatccaccg aggcgatgat ctcctcggga
     1561 acatgcgggc cgggcatgag ataggtgttg gtcatccgcg gaagcgggcg atgggcgtag
     1621 gattcgcgcc tgccgctgcc ggtcgaggcg acgcccgcga gctgcgcgtt caagcgatcg
     1681 taaagaaagc ctttcagcac gccattctcg atcaacacgt tgcggcgggt gggtgtgccc
     1741 tcgtcatcca ccgcgagtga cccgcgccgc tcggcgagcg tgccgtcgtc gaccaccgtg
     1801 cagagcgcgc tcgcgacgcg ttctcccgtg cggcctgtga aggccgaggt gcccttgcgg
     1861 atgaagtccc cctcgaggcc gtggccgacc gcctcatgca ccagcacacc cggccacccg
     1921 gggccgagca ccaccggcat cgtgcccgcc ggtgccgcga ccgcctcgag agcgaccagc
     1981 gcctggcggg ccgcctcgcg ggcgaagccg agagcccgct cgccttcgcg gaaccaggca
     2041 tagccgaagc gcccgccgcc gccggcgtgt ccctgctcac gccggccgtg atcctccgcg
     2101 atgacgctca cgttcacacg caccagcggg cgaacatccg cggccagggt gccgtcgctc
     2161 cccgcgatca gaatcacctc gtggaccgct cccaggctcg cggtcacccg cgtgattctc
     2221 ggatcggcgc gccgcgcctc gcggtccagt gcttcgagca gggccacctt ttctccggcg
     2281 gccagcgtcg cgatcggatc gatcggccgg tagagcctgg ccggcgcaga ttccgaccag
     2341 ccggcggttc ggtccgatcc gccgcgtgtc gcaatcgccc gcgccgagtc gcaggcggca
     2401 gcgagtgcgg gaaacgcgag ttcgtccgaa taggcaaagc cgctgcgctc gcccgcgatc
     2461 gcacgtacgc cgacgccacg ttccacactc gaggcgccct cgcgcacgat gccgtcctcg
     2521 agcatccagt gctcctcgcg cagaagctgg aaatagagat cggcgtagtc gacccgacgc
     2581 gccatgagcc ggccgaggag gcgctcgagc gccctctcgt cgagcgcgcc cggcgcgagc
     2641 aattcgcttt gtgcgagcgc gagattcgtc gccgccgcgg gtgtcgtcgg attcaccatc
     2701 gtcaatccat cctcaaaatt cgttcctcgt gattgtaaag gcgatcctgc atttcccggc
     2761 ggcgcgcgag cgtagcagtg tcgtcccgcc gcatgttggc tgacgcccag ccgggctatc
     2821 acaaacccgg ttcgagttcg cgccggcgag cctgttcgag cacggggaaa ccccttcgca
     2881 gccgtgcctg cccggacaga tcaatcggcg cctgtgccac cccggacctg cggcccaagg
     2941 tcgcgagagt ttctccccag ggcccaagaa tgcggcttcg gccgtaggtc tcgcggcctc
     3001 ccggatgccg gccggtttgc gccgcggcca ggagaaagca ctggttttcg agcgcgcggg
     3061 cgcgcagcaa actgtcccag tgtgcgctgc ccgtcgtcgc ggtgaaggcg gagggcaccg
     3121 cgaacaattg acagtcatgc ttcagcgcca acacccgaaa cagttccgga aagcgcaggt
     3181 cataacagac cgcaagcccg agtcgcccga gcggcgtctc gatcgcgacc agcgagcggc
     3241 cggcaacaaa gtggcgcgat tcgcggtagc gttcggcgcg acccgggacg gcaacatcga
     3301 agagatggaa tttgtcgtaa cgcgcaaccc gccgcccgtt ggcgtcataa acgagcaccg
     3361 cggcatgaaa tttcgtttcg cccgcgattc gaagcggcag cgttccgccg acgatccaga
     3421 gtctgcagtc cgccgccatc gcgccaagaa aatcctgcac gggaccctcg ccgtcctcct
     3481 ccgcggcccg gcggcgatcc gcggccgtcg cgggcatgca ggcgaaattt tcgggcagca
     3541 cggcgagcgt ggcgccggct tcggcggcac gctcgaggag ccgccgcgcc cggcgcagat
     3601 tcgaggcaac gtcggcgccc gaattcatct ggaggacggc cgccgatttc cccgtcaccg
     3661 cggcagcgac cggagcatcg ggactgcgcc tcgcgcggga gcggcgccgc tggccgccgc
     3721 gggcggcgac tggacatgcg gctgcggctt gagcggtggc gcgttgccga gcggtttgac
     3781 caccgggtcg cgaatgggac cgctgatcgt gtaatgaagt ttggtcagcg cggcaagcgg
     3841 gctgccgaac agcttggtga tcgcatacac caccgcgccg atgccgagcc ccccgacgat
     3901 cgcggcgatg acgggcagcg agccgaaata attcggagaa atttcggccc tttgagccat
     3961 ggggcccgcc gcgagattca ccggccccgt gagcttgatg ttcgcgacgg agctttcgat
     4021 gcgcagcccc ttggtgctcg cgatgccgcg gtcgatcacg tagtcgccgt aaatgcggct
     4081 gaacgggaac cccttgccga agaggttgtg gaagttgaac accag
//
LOCUS       DASUJG010000116         8038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1500647, whole genome shotgun sequence.
ACCESSION   DASUJG010000116 DASUJG010000000
VERSION     DASUJG010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8038
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500647"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..1160)
                     /locus_tag="VFX38_02335"
     CDS             complement(153..1160)
                     /locus_tag="VFX38_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003996740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX5313731.1"
                     /translation="MQDVSWLVWGVSIGLIIAILAFDLAFASIWPHRVGFREAALWSL
                     TYVAIAVGFGVWFAWIYGVESGTEYFAGYIIEKSLSVDNVFVFVIIMTAFAVPEEWQH
                     KALAFGIALALVMRAIFIALGAALISTFSFMFLFFGLLLIYTAVQLFRHRNEDPDIKN
                     SRIVKAARRVLPVTDRYLEGKLFARLDGRRAVTPLFIVLVAIGGVDLLFALDSIPAVF
                     GVTEEPYIVFTANAFALLGLRALFFLVKGLLDRLVYLSVGLSVILCFIGVKLILHWAH
                     QDIGGGVPEISTPVSLGVIVAILVIVTVASLIKVRTNPSMKAHAGSLRANKGAPPRDG
                     G"
     gene            complement(1226..2740)
                     /locus_tag="VFX38_02340"
     CDS             complement(1226..2740)
                     /locus_tag="VFX38_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX5313732.1"
                     /translation="MNLATVRARATAGIAAPAVRVEVHLSPGLPGLAIVGLPEAAVRE
                     ARERVRSALINTRFEWPARRITINLAPADLPKEGGRYDLAMALGLLVASGQLRPVAPL
                     TDFEFLGELSLSGELHPVKGVLPAALAARRARHALVVPAANGAEATRVAGLQVFAAGD
                     LPTLVAGLAGNGALEPLDAAQFPAAPPVSYPDVSEVRGQARAKRALELAAAGGHSLLM
                     VGPPGSGKSMLAERLPGLLPPLDDKEAIESAAIASVSAEGFDAETWGQRPYRAPHHSA
                     SGAALVGGGSHPRPGEVSLAHHGVLFLDELPEFDRHVLEVLREPLESGRIVISRAARQ
                     AEFPARFQLVAAMNPCPCGYLGDPRGRCRCTPDQIARYRGRISGPILDRIDLHVEVPR
                     PQAEALAPDRGPDGEASATVAARVAAARARQIERAGKPNAALGVAEVNRDCRPDDAGR
                     TLLEQASARLGLSARGYHRILKLARSIADLAATDAITPAHVAEAIGFRVLDRNV"
     gene            complement(2786..3067)
                     /locus_tag="VFX38_02345"
     CDS             complement(2786..3067)
                     /locus_tag="VFX38_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016352645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEX5313733.1"
                     /translation="MPADPHLLDDLAKRLAASLPASLRQAKEDIEHNLRAALEAALTR
                     LDLVSREEFDAQTALLRRTRERLDALAAEVEKMEHGGGEDHGAAGGAPH"
     gene            3204..3542
                     /gene="glnK"
                     /locus_tag="VFX38_02350"
     CDS             3204..3542
                     /gene="glnK"
                     /locus_tag="VFX38_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX5313734.1"
                     /translation="MKLITAIIKPFKLDDVREALADAGIEGMTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKLKIEVAVAEAQLERAMEAIQSAARTGKIGDGKLFVSPLEQVLRI
                     RTGEADEAAL"
     gene            3526..4005
                     /locus_tag="VFX38_02355"
     CDS             3526..4005
                     /locus_tag="VFX38_02355"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEX5313735.1"
                     /translation="MKPRCKLAARALFLCCGLAASAAVFAAAPGGDTTVYTWVDSQGV
                     RHYSDQPDGDGAKAISVAAPAPISAPAVVAAPPRSSKKPASASASTPSRPPPSATLGS
                     ARCAQLRMQVEALEPARRAVVTLNGKTRHLSGEDLARFKDALRARMQSACAAADSSG"
     gene            4089..5981
                     /gene="speA"
                     /locus_tag="VFX38_02360"
     CDS             4089..5981
                     /gene="speA"
                     /locus_tag="VFX38_02360"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEX5313736.1"
                     /translation="MNDIPVSPVPPEADAALARYSIGRWGAGYFGVNAAGHLTVRPRK
                     DGVEIDLADLAARLVADGFQLPVLVRFGDILRRRIAELTDAFNAARNAYGYSAPYTVV
                     YPIKVNQERHVIREIVRVGPDRLGLEAGSKPELLAVLGLARRGSLIIVNGYKDRECIR
                     LALAGQLAGYRVYVVIEKPDELELLLEEAAAMRVEPTLGIRVRLANALVGKWQDTSGE
                     KSKFGLTATELIAAIERLRAAGKLGWLRLMHVHLGSQIANIRDIQRGMAEVARYYTEV
                     RALGAPIEVVDVGGGLGIDYEGSRSRSDCSANYGFGLYAEVIVRTLAEVAGEAGQPVP
                     AIVSESGRALTAHHAVLVTKVIETERAPDAPAAAPADDAPAALRNLWEAYEYSGEGNP
                     QEIQADAAYEISEIHSLFSHGLVSLAERAHAEAIYAALCRRVLARINPTRASDWEFAD
                     HLVEKLADRMFINLSIFQSLPDAWAIDQVFPIVPLSRLNEVPTRRAILHDLSCDSDGR
                     IEAFVDNEGVEKTLAVHEPDGNPYLLGIFMVGAYQEILGDMHNLFGDTNAVNVLTRRD
                     GYIIRDTREGDSAQVMLRYVHIPPRALARTWRRRLAKSGLDSSRQAELAMLLERGLKA
                     YTYLDR"
     gene            5978..7051
                     /locus_tag="VFX38_02365"
     CDS             5978..7051
                     /locus_tag="VFX38_02365"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX5313737.1"
                     /translation="MNDDPVVLAPPATTGFLPDLCSPVATLPLVLIAALVALALAVAR
                     AAGPGFWPEFGRLLLFCEVLTLASAAVLCLLRRLLSGARPVLLAAFAFAALLATDWVI
                     SEGAWFLLATLGVPLPRGAQARLWWVLRIVVVAAIVDALVLRYLYLGASWRENVRREA
                     ASRLAALTARIRPHFLFNTLNTAIALVAERPQDAERSLENLAELFRASLADQDSRIAF
                     AEELAIVRRYLAIEALRLGSRLTVHWRLAPLVERALVPGFILQPLVENAVTHGIEPAA
                     AGGSLEIRAERAGRMLELAVDNSIGADSGGEGYGIGLSGIRARLALVYGGDATLAVER
                     SPGRFGVRLRCPWEECDADPDRG"
     gene            7029..7754
                     /locus_tag="VFX38_02370"
     CDS             7029..7754
                     /locus_tag="VFX38_02370"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX5313738.1"
                     /translation="MQILIADDEAPARSRLRRMIGELAAGEIVGEAASGREVLALAAS
                     RRPDVVLLDIRMPEGDGIAVARSLTKLEPPPAVVFVTAFADRALPALEAGAAAYLVKP
                     VSREALAAALVRASRPSRAQLTALAAEGAVPRHLTVRVGRESRRIAFADLRYLRAENK
                     RTLVAYASGEAVLDDSLAALEREFAAQLLRIRRNLLVARAAIASVIHEPAGTIVVLGS
                     GERLPVARRHRRALASALAESRP"
     gene            7751..>8038
                     /locus_tag="VFX38_02375"
     CDS             7751..>8038
                     /locus_tag="VFX38_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX5313739.1"
                     /translation="MTFVLATRRSRLALRQTAEVAALLRARHADLEVVPLALSTRGDE
                     ILDRSLAPLGGKGLFVKALERALADGRARAAVHSLKDVPTVLSPGFVLAAVA"
BASE COUNT         1331 a         2682 c         2699 g         1326 t
ORIGIN      
        1 gcagttcttc ttcctgcggc acggcaaggt cgtgaagcac atcccgggat atgacaaaaa
       61 cctgatttcg gaaatcgaat cgggtttgaa aaccatcggt gtgaacccat agcagcggtg
      121 ttgtccgcgc ccgattgtcg cacaggcgaa tgctacccgc catcgcgcgg gggagctccc
      181 ttgtttgcgc gcagagatcc agcatgtgcc ttcatggatg ggttggtgcg cacctttata
      241 aggcttgcaa cagtcacaat gaccagtatc gccacgatga cgccgaggct gacgggagtg
      301 ctgatttcgg gtacgccccc gccaatgtcc tgatgggccc agtgcagaat caacttcacc
      361 ccgatgaaac acagaatgac agacaggccg acggacaaat aaaccaatct gtccagcaag
      421 cccttgacca ggaagaacaa ggcacgcagt cccaacaatg cgaaggcatt ggcggtgaaa
      481 acaatgtaag gctcctcggt tacgccgaac actgccggga tggaatcgag cgcgaacaac
      541 agatcaacgc cgccaatggc gaccagcacg atgaacagcg gcgtaactgc ccggcggcca
      601 tcgagccggg caaacagctt cccttccagg taacgatcgg taaccggcag cacgcggcgt
      661 gctgccttga caatcctgct gtttttgata tcggggtcct cgttccgatg acggaagagt
      721 tgcacggccg tgtagatcag aagcagtccg aagaacagga acatgaagga aaacgtcgaa
      781 atcaaggcgg cgcccaacgc gatgaagatg gcgcgcatga ccaatgcgag tgcgatgccg
      841 aaagcaagcg ccttgtgctg ccattcttca ggtaccgcga aagccgtcat gatgatgacg
      901 aagacaaaca cgttgtcaac ggacaggctt ttttcgatga tatagccggc gaaatattcc
      961 gtaccggatt ccacgccgta gatccaggca aaccagacac cgaaccccac cgcaatggcg
     1021 acataggtga gcgaccagag agccgcctcc ctgaaaccta ctctatgggg ccagatggac
     1081 gcaaaggcca agtcgaaggc cagaatcgcg ataatcaggc cgattgatac gccccagaca
     1141 agccagctta cgtcctgcac cgtttattcg cccggcttca cgatttcaca acaacagact
     1201 tcgtcagatt atccgggaca gcttgttaaa cgttccggtc gaggacgcga aagccgatgg
     1261 cttcggcgac gtgcgccggc gtgatcgcat cggtggcggc gaggtctgcg atgctgcgtg
     1321 cgagtttcag gatccgatga tagccgcggg cggacagccc cagccgcgcg ctcgcctgtt
     1381 cgagcagcgt gcgaccggcg tcgtcgggcc gacagtcgcg attgacttcg gcgacgccga
     1441 gcgcggcgtt gggctttccc gcacgttcga tctggcgtgc ccgcgccgcc gcgacccgtg
     1501 cggcgaccgt cgcgctcgcc tctccgtccg gtccgcgatc gggcgcgagc gcctcggcct
     1561 gcgggcgcgg cacctcgacg tgcagatcga tgcgatcgag aatcggtccg gagatgcgtc
     1621 cgcgatagcg cgcgatctga tccggcgtgc agcggcagcg cccgcgcgga tcgccgagat
     1681 aaccgcaggg gcagggattc atcgcggcga cgagttgaaa gcgagccgga aactcggcct
     1741 ggcgtgcggc gcgcgagatg acgatgcgcc ccgattccag cggctcgcgc aggacttcga
     1801 gcacgtggcg atcgaattcc ggaagttcgt cgagaaacaa aacgccgtga tgggcgagcg
     1861 agacttcgcc cggtcgcgga tgcgaaccgc cgccgaccaa cgccgcgccc gaggcgctgt
     1921 ggtgcggcgc gcgatacgga cgctggcccc aggtttcggc gtcgaaccct tcggcgctca
     1981 ccgaggcgat cgcggccgat tcgatcgctt ccttgtcatc gagcggcggg agcagtcccg
     2041 gcaggcgttc cgcgagcatg ctcttgccgc ttccgggagg tccgaccatg agcaggctgt
     2101 ggccgcccgc ggcggcgagt tcgagcgcgc gtttggcgcg tgcctgaccg cgcacctcgg
     2161 agacgtccgg ataggaaacc ggcggcgcgg caggaaactg tgctgcgtcg agcggttcga
     2221 gagcgccgtt gccggcaagg ccggcgacga gggtcggcag atcgcccgcg gcgaagactt
     2281 gaagtccggc gactcgggtc gcctcggcgc cgttggcggc cggcaccacg agcgcatgac
     2341 gagcgcggcg ggcggcgagc gccgccggaa gcacgccctt caccggatgc agctcgccgg
     2401 aaagtgacag ctcgcccaga aattcgaagt cggtgagcgg tgcgacgggc cggagctgtc
     2461 ccgatgcgac gagcaagccg agcgccatcg ccagatcgta gcgtccgcct tccttgggaa
     2521 gatcggccgg ggcgagattg atggtgatgc gccgcgccgg ccattcgaag cgcgtgttga
     2581 tgagggcgct gcgcacgcgt tcgcgggcct cgcgcacggc cgcttcggga aggccgacga
     2641 tcgcgagtcc cggaaggccg ggcgagagat gcacctcgac gcgtacggcc ggggcggcga
     2701 tgcccgccgt tgcgcgcgca cgcacggtcg cgagattcat cgggcactct cgcgccgcgc
     2761 gcccgccgcg gcgagcgacg cgatgctagt gcggggctcc cccggctgcg ccatgatctt
     2821 ctcctccccc gtgttccatc ttttccactt cggccgcgag tgcgtcgagg cgctcgcggg
     2881 tgcgccgcag cagcgccgtc tgagcgtcga attcttcgcg gctcaccagg tcgagacggg
     2941 taagcgccgc ctcgagcgcc gcgcgcagat tgtgttcgat atcctccttc gcctgacgga
     3001 gcgaagccgg aaggctcgcc gcgaggcgtt tggcgagatc gtcgagcaga tgcggatcgg
     3061 cgggcatggc ggctccttcc tccggcggcg ttccatgagt gtagccgatg ccggcgcagg
     3121 cacgcggatc ggatgcgatg ggcccggtcc cggcatcggt gatgcagcgg tgctacactc
     3181 aagcgccgcc gggggcgacg ttcgtgaaac tgattacggc gatcatcaaa cctttcaagc
     3241 tcgacgacgt gcgcgaggcg ctcgccgacg ccgggatcga gggcatgacc gtcaccgagg
     3301 tcaagggatt cggccgccag aaggggcata cggaactcta tcgcggagcg gaatacgtgg
     3361 tcgactttct gcccaagctc aagatcgagg tcgccgtcgc cgaagcgcaa ctcgaacgcg
     3421 cgatggaagc gatccagtcc gccgcccgca cgggcaagat cggcgacggc aagctcttcg
     3481 tctccccgct cgagcaggtg ttgcgcatcc gcacgggaga agcggatgaa gccgcgctgt
     3541 aaactcgccg ctcgggcgct gttcctctgc tgcggactcg cggcgagcgc agcggtcttc
     3601 gccgccgcgc ccggcgggga caccaccgtg tacacctggg tggactcgca gggagtgcgc
     3661 cattactccg accagcccga cggcgacggc gcgaaggcga tttcggttgc cgctcccgcg
     3721 ccgatttccg caccggccgt cgtcgcggcg ccaccgcgaa gttcgaagaa accggcatcg
     3781 gcatcggcgt cgacgccgtc gcgtccgccg ccgagcgcga cgctcggctc ggcgcgctgc
     3841 gcgcaactgc gcatgcaggt cgaggcgctc gaaccggcgc gccgcgcggt cgtcaccttg
     3901 aacgggaaaa cccgccatct ctccggcgag gatctcgcgc gtttcaagga tgcgcttcgc
     3961 gcccgaatgc agtcggcctg tgccgcggcc gattcctccg ggtagcgaac gccaacgcga
     4021 cggcagggcg agggcgcgca ctccgaagca agtacaatgc agattcccgc tcctgatccg
     4081 gcagcgacgt gaacgacatc cccgtttctc ccgtgccacc cgaggccgat gccgcgctcg
     4141 cgcgctacag catcggacgc tggggagccg ggtatttcgg cgtcaatgcc gccgggcatc
     4201 tcacggtgcg tccgcgcaag gacggcgtcg agatcgatct cgccgacctc gccgcgcgcc
     4261 tcgtcgccga cggcttccag ttgccggtcc tcgtgcgctt cggcgacatc ctgcgccggc
     4321 gcatcgcgga actcaccgac gccttcaatg ccgcgcgcaa tgcctacggt tattccgcgc
     4381 cgtacacggt cgtctatccg atcaaggtga atcaggaacg ccacgtcatc cgcgaaatcg
     4441 tgcgcgtggg gccggaccgt ctcggcctcg aagccggaag caaaccggaa ctgctcgcgg
     4501 tgctcgggct tgcgcgtcgc gggagcctca tcatcgtcaa cggatacaag gatcgcgagt
     4561 gcatccgtct cgcgctcgcc ggacaactcg cgggctatcg cgtctatgtg gtgatcgaga
     4621 agcccgacga actcgagctg ctgctcgagg aggcggcggc gatgcgggtc gagccgacgc
     4681 tcgggattcg cgtgcggctc gccaacgcgc tcgtcggcaa atggcaggac acgagcggcg
     4741 agaaatcgaa attcggactc acggcgaccg aactcatcgc cgcgatcgaa cggctgcgtg
     4801 ccgcgggaaa actcggctgg ctgcgcctga tgcatgttca tctcggatcg cagatcgcga
     4861 acattcgtga cattcagcgc ggcatggccg aggtcgcccg ctattacacc gaggtgcgcg
     4921 cgctcggcgc gcccatcgaa gtggtggacg tcggcggcgg cctcggcatc gactacgagg
     4981 gcagccgttc gcgcagcgac tgctcggcca actacggctt cggactctat gccgaggtga
     5041 tcgtgcgcac gctcgccgag gtcgccggcg aggcggggca accggtgccc gcgatcgttt
     5101 ccgaatcggg gcgcgcgctc accgcgcatc atgcggtgct cgtcaccaag gtgatcgaaa
     5161 ccgaacgcgc gcccgacgcg ccggccgcgg ctcccgcgga tgacgcgccg gcggcgctgc
     5221 gcaatctctg ggaggcctac gaatactcgg gcgagggcaa cccgcaggaa atccaggccg
     5281 atgccgccta tgagatcagc gagattcatt cgctcttcag tcacggactc gtgtcgctcg
     5341 ccgagcgcgc gcatgccgag gcgatctacg cggcactctg ccggcgggtg ctcgcgcgca
     5401 tcaatccgac ccgcgcctcg gattgggagt tcgccgatca tctcgtcgag aaactcgccg
     5461 atcgcatgtt catcaatctg tcgatcttcc agtccctgcc cgacgcctgg gcgatcgatc
     5521 aggtgtttcc gatcgtgccg ctttcgcggt tgaacgaggt gccgacgcgg cgcgcgatcc
     5581 ttcacgatct ctcctgcgat tccgacgggc gcatcgaggc cttcgtggac aacgaggggg
     5641 tggagaagac gctcgcggtg cacgagcccg acggcaatcc ctatcttctc ggcatcttca
     5701 tggtcggcgc gtatcaggaa atactcggcg acatgcacaa tctgttcggc gacaccaacg
     5761 cggtgaacgt gctcacgcgc cgcgacggct acatcatccg cgacacccgc gagggcgaca
     5821 gcgcgcaggt gatgctgcgc tacgtgcaca tcccgccgcg ggcgctcgcg cgcacctggc
     5881 ggcggcggct cgccaaaagc ggtctcgatt cttcgcgtca ggcggaactc gcgatgctgc
     5941 tcgaacgcgg tctcaaggct tacacctacc tcgatcgatg aacgacgatc cggtggtgct
     6001 cgctccaccg gcgaccacgg ggtttctgcc ggatctgtgc tcgccggtcg cgacgctgcc
     6061 gctcgtcctc atcgcggcac tggtcgccct cgcgctcgcg gtggcgcggg cggccggccc
     6121 cggattctgg ccggagttcg ggcggctgct tctcttctgc gaagtcctga cgctcgcaag
     6181 cgcggcggtg ctctgtctgc tgcgtcgcct gctttccggt gcgcgacccg tcctcctcgc
     6241 ggcgttcgcc ttcgccgcgc ttctcgcgac cgactgggtt atttcggagg gcgcgtggtt
     6301 cctgctcgcg acgctgggcg ttccactgcc gcgcggtgcg caggcgcgct tgtggtgggt
     6361 gttgcgcatc gtggtggtcg ccgcgatcgt cgatgcgctg gtgctgcgat atctctatct
     6421 cggcgcgagc tggcgcgaga acgtgcgccg cgaagcggcg agccgtctgg ccgcgctcac
     6481 ggcgcgcatc cgtccgcatt ttctcttcaa cacgctcaac accgcgatcg cactcgtcgc
     6541 cgaacggccg caggatgccg agcgcagtct cgaaaatctc gccgaactgt ttcgcgcgag
     6601 cctcgcggat caggattcgc gcatcgcatt cgcggaagag ctcgcgatcg tacgccgcta
     6661 tctcgcgatc gaggcgttgc gactcggctc gcgtctcacc gtccactggc ggctcgcgcc
     6721 gctggtggaa cgtgcgctcg ttcccggctt tatcctgcaa ccgctggtgg agaatgccgt
     6781 gacgcacgga atcgaacccg cggccgcggg gggatcgctc gagatccgcg ccgagcgcgc
     6841 cgggcgcatg ctcgaactcg ccgtggacaa ttcgatcggc gccgacagcg gcggcgaagg
     6901 ctacggcatc gggctttcgg gcattcgcgc ccggctcgcg ctcgtttacg gaggggatgc
     6961 gacgctcgcc gtcgagcgtt cccccggaag attcggcgtg cgcctgcgtt gtccctggga
     7021 ggaatgcgat gcagatcctg atcgcggatg acgaggctcc cgcgcgttcc cgtctgcgcc
     7081 ggatgatcgg cgaactcgcc gcgggggaaa tcgtcggcga ggcggcgagc gggcgtgaag
     7141 tgctggcgct cgccgcgagc cggcgccccg acgtcgtgct gctcgacatc cgcatgcccg
     7201 aaggcgacgg catcgcggtg gcccgcagtc tgacgaaact cgaaccgccg cccgccgtgg
     7261 tattcgtgac ggcctttgcg gatcgggccc tgcctgcgct cgaagcggga gccgcggcct
     7321 atctcgtcaa acccgtgagc cgcgaggcgc tggcggctgc gctcgtgcgt gcctcgcggc
     7381 catctcgcgc ccagttgacg gctctcgccg cggagggcgc cgtgccgcgg catctcaccg
     7441 tgcgcgtggg acgcgagtcg cggcgcatcg ccttcgcgga tctccgctat ctgcgcgcgg
     7501 aaaacaagcg cacgctggtt gcgtatgcga gcggggaggc ggtgctcgac gattcgctcg
     7561 cggccctcga gcgcgaattc gccgcgcagc ttctgcggat ccgccgcaat ctgctcgtgg
     7621 cgcgcgcggc gattgcctcg gtaattcacg aacccgcggg aacgatcgtc gtgctcggct
     7681 cgggcgagcg ccttccggtc gcccgccgcc accgccgcgc tcttgcgagc gctcttgccg
     7741 aaagccggcc atgacgttcg tgctcgccac ccgccgcagc cgcctcgcgc tgcggcagac
     7801 cgcagaagtt gccgccttgc tgcgtgcgcg gcacgcggac ctcgaggtcg tgccgcttgc
     7861 gctcagcacc cgcggcgacg aaattctcga tcgttcgctc gcgccgctcg gcggcaaggg
     7921 gctcttcgtc aaggcgctcg aacgggcgct cgccgatgga cgcgcgcgcg ccgccgtgca
     7981 ttcgctcaaa gatgtgccga ccgtgctcag cccggggttt gtgctcgcgg ccgtcgcg
//
LOCUS       DASUJG010000117         2306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_32786, whole genome shotgun sequence.
ACCESSION   DASUJG010000117 DASUJG010000000
VERSION     DASUJG010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32786"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VFX38_02380"
     CDS             complement(<1..214)
                     /locus_tag="VFX38_02380"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HEX5313740.1"
                     /translation="MAAVAPDEAFGLDWPYPLFGYQKEGIEKLLDGRSVLLTDEMGLG
                     KTIQAIAAIRILVTRKEIARALIVCPV"
     gene            complement(221..1090)
                     /locus_tag="VFX38_02385"
     CDS             complement(221..1090)
                     /locus_tag="VFX38_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313741.1"
                     /translation="MKPATEPYDRLESWSTEEARKGSLSALFAALLWLIPRLAPDEHA
                     RIARFFGGGRLNATAVKSWLRAHGAAKLFDLKLAREAELVRQRLRFAPRLLASIGLAG
                     WCILLDEAELIGRYSPLQRGRSDAELARWLGLDEADALPGIATVAAFADDYVDEMFDH
                     RRDEELIPQVLERKGLERQVVLARNCMAELRRRRGVRLVAPDETALRAAQTRIAELYG
                     AAYHWNPSDLAPGERLAAKSIRQYIKSWITQWDIERLYGERSAIEARTLQTDYTESAE
                     LERAAPAADTDEE"
     gene            complement(1056..1475)
                     /locus_tag="VFX38_02390"
     CDS             complement(1056..1475)
                     /locus_tag="VFX38_02390"
                     /inference="COORDINATES: protein motif:HMM:NF022376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system ATP-binding domain-containing
                     protein"
                     /protein_id="HEX5313742.1"
                     /translation="MTNRPPIEARGAIEALRAGVPNRAAIRQLGEKDGGLVNSFLDSL
                     SGCTKLLEQGRQAEGTLVWGGFGAGKSHLLGFMRELALERNFVVSLVPVSKETPMFDP
                     ARLFAAAIRAAEVPGVNDDVMTGALAIEAGDRALRSP"
     gene            complement(1472..2287)
                     /locus_tag="VFX38_02395"
     CDS             complement(1472..2287)
                     /locus_tag="VFX38_02395"
                     /inference="COORDINATES: protein motif:HMM:NF022376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system ATP-binding domain-containing
                     protein"
                     /protein_id="HEX5313743.1"
                     /translation="MAVCRVDASQTRLHMIQGIFFAISRSLDWPGMAQTFLEAMVTKN
                     GYAWPKPGGAVTFRDVAAFNGIEERLFRRELNRWMTEEVMRDRSMSQDFRIAMTQLCL
                     TRLEPADANAGVTAPVLEWLRGELRAIGALRQTFITSKITRHNARAMLHSLCRWLRLC
                     ERAGICVTLDIRQVLRAVSIPGEGIRYTPAAVIDAFEVLRQLIDDAEAFEGLLVIALA
                     DDALTGEDPKRSLNAYDALKMRVSDDVRATGRDNPLAALVRLESSRLQTEAGP"
BASE COUNT          415 a          756 c          722 g          413 t
ORIGIN      
        1 tgactgggca gacgataagc gcccgcgcaa tctccttgcg cgtcactaga atgcggattg
       61 ctgcgattgc ctggatcgtt ttgcccagtc ccatctcgtc ggtgagcagc accgaccggc
      121 cgtcgagcag cttttcgatt ccctccttct gatagccgaa tagcggatac ggccaatcca
      181 gcccgaacgc ctcgtccggc gctacggccg ccaccccgaa ctattcctcg tcggtgtcgg
      241 cggcgggagc ggcccgttcc agttctgcgc tttccgtgta gtcggtttga agcgtgcgcg
      301 cctcaatggc cgaccgttcg ccgtacaagc gctcgatatc ccactgggtg atccaggact
      361 tgatgtattg gcggatcgac ttggcggcca gccgttcgcc aggtgcgaga tcggaagggt
      421 tccagtggta ggcggcgcca tagagttccg cgatccgggt ctgagcggcg cgcaaagcgg
      481 tttcgtcagg ggcgaccagt cggaccccgc gcctccgccg caattccgcc atgcagtttc
      541 gcgcgaggac gacttggcgc tccagcccct tccgctccag gacctgcggt atgagttcct
      601 cgtcacgacg atggtcgaac atctcgtcaa catagtcgtc ggcgaatgca gcgaccgtgg
      661 cgattccagg cagggcgtcc gcttcgtcca aaccgagcca gcgcgccagc tcggcatcgc
      721 ttctgccgcg ctgcaaaggc gaataccggc cgatgagctc ggcctcgtcc agaagaatgc
      781 accagccggc aagaccgatt gaagccaaaa gacgcggtgc gaaccgcagc cgctggcgaa
      841 cgagttcggc ttcgcgagcc agcttgagat caaacagctt ggctgcgccg tgggcgcgca
      901 gccaggactt caccgccgtc gcattgagtc ggccgccgcc aaaaaaacgc gcgatgcgcg
      961 catgttcgtc gggcgcaagc cgcggaatca gccaaagcag ggccgcgaac agagccgaca
     1021 gcgatccttt gcgagcctct tccgtcgacc atgattcaag gcgatcgtaa ggctctgtcg
     1081 ccggcttcaa tcgccagagc gccggtcatc acgtcgtcgt tgacgccggg aacttccgcc
     1141 gcgcggattg ccgcggcgaa caggcgagcc ggatcgaaca tcggcgtctc cttgctcacc
     1201 ggcacgaggc tgacgacgaa gttccgctcc agggcaagct ctcgcatgaa gcccaagagg
     1261 tgcgatttgc ccgcgccgaa tccgccccat acgagggttc cctccgcctg tcgtccttgt
     1321 tcgaggagct tcgtgcagcc gctaagcgaa tcgaggaacg agttgaccag gcccccgtcc
     1381 ttttccccca gttggcgtat ggctgcgcgg ttcggaactc cggcgcgcag ggcttcaatt
     1441 gcgccgcgcg cctcgatcgg cggccggttc gtcatggccc ggcctcggtc tgcaagcgac
     1501 tcgactccaa ccgcacgagg gccgcgagcg gattgtcgcg gccagtcgcg cggacgtcgt
     1561 ccgacacccg cattttcagg gcgtcatagg cgttgagcga tcgtttgggg tcttcgccgg
     1621 tgagcgcatc gtccgcgagc gcgataacga gcagaccttc gaaagcctcg gcgtcgtcga
     1681 tcagctggcg cagcacctcg aacgcgtcga tcacagccgc cggggtgtaa cgaatacctt
     1741 cgccggggat gctcacagcg cgaaggactt gacgaatgtc gagcgtgacg caaatccccg
     1801 cgcgctcgca aagccgcaac cacctgcata gggagtgcaa catcgcgcgt gcgttgtgtc
     1861 tcgtgatttt ggaggtgatg aaggtctgac gaagggcgcc gatcgcccgc aactcgccgc
     1921 ggagccattc aagcaccggc gcggtgacgc cggcgttcgc gtccgcgggt tcgagccttg
     1981 taaggcagag ttgggtcatg gcgatgcgga aatcctggct catcgaccga tcgcgcatga
     2041 cctcctcggt catccagcgg ttcaactcgc gacggaacaa acgctcctcg atcccattga
     2101 aggcggcgac gtcccggaaa gtcacggcgc cgccaggctt cggccacgcg tagccgttct
     2161 tggtcaccat ggcctcgagg aaggtctgcg ccatcccggg ccagtcgagg cttcgcgata
     2221 ttgcgaagaa gataccctgg atcatgtgaa gcctggtctg ggacgcatcg acccggcaaa
     2281 ccgccatgtc gcctcgttcc gcaagc
//
LOCUS       DASUJG010000118         7224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1255210, whole genome shotgun sequence.
ACCESSION   DASUJG010000118 DASUJG010000000
VERSION     DASUJG010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7224
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1255210"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /gene="pth"
                     /locus_tag="VFX38_02400"
     CDS             complement(<1..511)
                     /gene="pth"
                     /locus_tag="VFX38_02400"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX5313744.1"
                     /translation="MSENVVRLVVGLGNPGVEYLATRHNVGFWFVDALAAELGAAFRA
                     TRRLHGESARATLGKAELLLLKPDTFVNRSGLAVRATLDYFKYEPSQLLVAHDDLDLA
                     PGTVRVKRGGGHGGHNGLRDIIAHVGADFARLRFGIRHPAAGAAVIDYVLGAPPRAEE
                     EEILTALGAA"
     gene            complement(533..1249)
                     /locus_tag="VFX38_02405"
     CDS             complement(533..1249)
                     /locus_tag="VFX38_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018175345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEX5313745.1"
                     /translation="MAAEFLLNAEPRTDAGKAASRRLRRDGRVPAVLYGGTQGAQALS
                     LDHNELLQNLKSEAFRSRVLTVKFGGASAQAIVKDLQAHPFKSEVLHVDLQRVDAGQK
                     IRMEVPLHFKGGDVAPGVKAGGVLSRSVVEVEIECLPKDLPEHLELDVSALEIGDALH
                     LSDIPLAAGVAIVALAHDDAHEHDVPVASVHLPRVSAEEEEAEAQAAAEGAEAAPAAE
                     AKAGEEPARKESAKKGESAA"
     gene            complement(1387..2325)
                     /locus_tag="VFX38_02410"
     CDS             complement(1387..2325)
                     /locus_tag="VFX38_02410"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX5313746.1"
                     /translation="MMVFAGRANPGLAADIASHLQLPLGKINVGRFSDGEVQVEIFEN
                     VRGRDVFVVQPTCPPVNENLMELLLIADALMRASAGRITAVVPYYGYSRQDRRPRSLR
                     VPISAKLVANLIAHAGVTRLLSVDIHADQIQGYFDIPFDNVHASPILLSDLWRCAYPD
                     IIIVSPDVGGVARARAIAKRMDDADLAIIDKRRSQPNESKVMNIIGDVRGRTCVIVDD
                     MVDTAGTLCHAAQALKDEGAVRVVAYITHPVLSGDAVSRIAGSALDQLVVTDTIALRP
                     DAAACPKIRQLSIADIMAETLRRISDEESVSSLYVD"
     gene            complement(2385..2458)
                     /locus_tag="VFX38_02415"
     tRNA            complement(2385..2458)
                     /locus_tag="VFX38_02415"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2424..2426),aa:Gln,seq:ttg)
     gene            complement(2449..3324)
                     /gene="ispE"
                     /locus_tag="VFX38_02420"
     CDS             complement(2449..3324)
                     /gene="ispE"
                     /locus_tag="VFX38_02420"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX5313747.1"
                     /translation="MEASPDALSAWPAPAKLNLYLHVTGRRADGYHELSTAFQFLDFG
                     DRLWFAPRDDGEFRLHDPVPGVAVEDDLVARAVRSLECAAGRSLGMDVRRDKRIPVGG
                     GLGGASSDAATTLVAVNALAGLGLDGERLAALALELGADVPVFVRGAAAHALGVGERL
                     FAAEFPEPWYVVIHPAVSVSTAAIFAAPELTRDSEPAKMSAFSLDEARNDCEPVVRRR
                     YPEIARALDWLRDRGPARLTGTGSCVFTWRDGRDAAAALAAEVPGPWRAIVARGRNRS
                     PLRERLRRERGNVGA"
     gene            complement(3300..3971)
                     /gene="lolB"
                     /locus_tag="VFX38_02425"
     CDS             complement(3300..3971)
                     /gene="lolB"
                     /locus_tag="VFX38_02425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HEX5313748.1"
                     /translation="MTGANAGIVGANRGAMRAEWFGRQLLPALAGLWLFLAGGCTTLL
                     YPSGPANPAAWQNRLPRLQNLDDWRLHGRIGVVTPGNGGSANVSWVQQGEEMSLSFSG
                     PFGLGAVSLWGTPSEMYLRDSQGHVQTTVTPETALERRLGWPIPVASLRYWVTGQPAP
                     GSPYQLRLDSRGLVIQLQQQGWTVAYEAYAPAAGLLLPVRLAASRPGGHIKLVVNDWK
                     LPPTP"
     gene            complement(3979..5643)
                     /locus_tag="VFX38_02430"
     CDS             complement(3979..5643)
                     /locus_tag="VFX38_02430"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5313749.1"
                     /translation="MARSGLVPAAVAALALAGCAAPPAAQRAPAASSVAAPASAGAAL
                     ATAQQLHSPRLTAMVLLDQARDKHDANQALLAALFATEAGDYALATQAAHFAHSLEPK
                     HPEPFAIEVRIALDQGQIAAAREALAEAYALGGADAVQLSLTGPIDPWFVLSVLQPLA
                     KANPEDTKLQLAYAQAALVAGSASEALRAAQAAGATGGAAAGTIEIQARWDLGQRQQA
                     IAMLDRMLASAPHDVTLRLFAAGLLARANQTGRAREVLGDARALAPNNAHVALAEAIV
                     DMVAGEEQAARSRATRMLEKGSDEAGVYNLLGNMAAAEEDWAEAFGWYQGITDSDFLA
                     SAQTGAVLALAQWKGLAPALDYIKRLRDAFPALDPTWVGLDSAVLSQAGRTQDAYAVI
                     ADGAKRYPGVPPLRYQQALLADQLGQGATALQLLHELAGEEPENPDYLNAYGYTLTEH
                     SRRYREAYGYIVRALTLAPDDGAILDSMGWVLFRMGEAKKALPYLERAWELTGDSDVA
                     NHLVQLYLALGNPAAAGKLLDDALAKAPHDTALLRLKQRMVKSATP"
     gene            5749..7008
                     /gene="hemA"
                     /locus_tag="VFX38_02435"
     CDS             5749..7008
                     /gene="hemA"
                     /locus_tag="VFX38_02435"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX5313750.1"
                     /translation="MLFVVGVSHKTAPLALRERLAVAPARVPELLDAMLEGGDLREAV
                     LLSTCNRSELYAVTEAHRKAAVLASFRAIGDCDEEILPGCYVEEGPGAARHLFRVAAG
                     LDSMVLGEAHILGQVRAAYLTAHRASAVGPELHQLFQHVLAATKNIRSDSSLDGLRSL
                     PYAAAKLARERLGKFGERRAVLIGAGKTIDALAFHLRGEVSRLQIANRSPQAARALAA
                     RHGAEAILFADLPEALAEADLLASATTSETPVVSADMLHKRTGTRSLVVLDLAVPRDV
                     AADVSLLAGVEVIGLDDLAAVIEASAELRYAAAEQAETAVEGALAGWQRARRIRDAVP
                     TICALRAEASLARRQSLAAARRIAATRGSDEALEYLATTLTNRLIHAPTARLRAAAAA
                     DEATIIATARELFGLDNKGAQEDSAAA"
     gene            7005..>7224
                     /locus_tag="VFX38_02440"
     CDS             7005..>7224
                     /locus_tag="VFX38_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313751.1"
                     /translation="MNPLLLARLQALGERARILARELEQPQTAAEPRRFVALSREYSR
                     LASAERGLAEYESLDKALAEATRLAEDGD"
BASE COUNT         1158 a         2449 c         2477 g         1140 t
ORIGIN      
        1 gcgccgcgcc gagtgcagtg agaatctcct cttcttcagc ccgcggcggc gccccgagaa
       61 cgtagtcgat gacggcggcg cccgccgccg gatggcgaat gccgaagcgc aggcgcgcga
      121 aatccgcgcc gacgtgggcg atgatgtcgc gcagcccgtt gtgtccgccg tgtccgccgc
      181 cgcgtttgac ccgcaccgtg cccggcgcga gatcgagatc gtcgtgggcg acgaggagtt
      241 gcgacggttc gtacttgaag tagtcgagcg tggcgcgcac cgcaaggccg ctgcggttga
      301 cgaaggtgtc cggcttcagc agcaggagtt cggccttgcc gagcgtcgcg cgagcgctct
      361 cgccgtgcag gcgccgcgtg gcgcgaaacg ccgcgccgag ttccgcggcg agcgcatcga
      421 cgaaccagaa gccgacgttg tggcgggtcg cgagatactc gacgccgggg ttgccgagtc
      481 cgacgaccag gcgaacgacg ttttccgaca cggtgcagtc ggcgcgccgc gcttacgccg
      541 cggactcgcc ctttttggcc gactctttgc gcgccggctc ttcgcccgcc ttcgcctcgg
      601 cggcgggagc ggcctcggca ccctcggccg cggcctgcgc ctcggcttcc tcttcctcgg
      661 cgcttacccg cggcagatgc acggaggcga ccggcacgtc atgttcgtgc gcatcgtcat
      721 gggcgagcgc gacgatcgcg actcccgccg cgaggggaat gtcggaaaga tgcagggcat
      781 cgccgatttc gagcgccgag acatccagct ccagatgctc gggaagatcc ttcggcaggc
      841 actcgatttc gacttcgacg accgagcgcg agagcactcc gccggccttg acgccgggcg
      901 cgacgtcgcc gcccttgaaa tgcagtggca cttccatgcg gatcttctgc cccgcatcca
      961 cccgctgcaa atccacgtgc agcacctcgg acttgaaggg atgggcctgc agatccttca
     1021 cgatcgcctg cgcgcttgcg ccgccgaact tcacggtgag tacccgcgag cggaaggcct
     1081 cgctcttgag attctgcagg agttcgttgt gatccaggct cagcgcctgc gcaccctgcg
     1141 ttcctccgta caggaccgcc ggaacccgcc cgtcgcggcg caggcggcgg ctcgcggcct
     1201 tgcccgcgtc ggttctcggt tcggcgttca gcaaaaattc cgcggccatg tcgtgggtac
     1261 ctcgaaaagg cgaatcggcg agttggaacc gggtatttta cagggtttgg gcgccgggga
     1321 ccgccggccg ggccggcacc ggcgaaacac cgccctccgc agattcaccc agctctgcct
     1381 tcccgttcaa tccacgtaga gcgaggaaac ggattcctcg tcgctgatgc gccgcagggt
     1441 ctcggccatg atgtcggcaa tcgaaagctg gcggatcttc gggcaggcgg cggcatcggg
     1501 gcgcagcgcg atcgtgtccg tcacgacgag ctgatccagg gcggagccgg cgatgcgcga
     1561 aacggcatcg cccgagagta ccggatgagt gatgtaggcg acgacccgga ccgcgccctc
     1621 gtccttcaag gcctgcgccg cgtggcagag cgttccggcg gtatccacca tgtcgtccac
     1681 gatcacgcag gtgcgcccgc gcacatcgcc gatgatgttc attactttcg attcgttcgg
     1741 ttgcgagcgg cgcttgtcga tgatcgcgag atcggcgtcg tccatgcgtt tggcgatagc
     1801 acgcgcgcgg gcgactcccc cgacgtcggg cgagacgatg atgatgtcgg gataggcgca
     1861 gcgccacaga tcggaaagca ggatcggcga ggcgtgcaca ttgtcgaagg gaatgtcgaa
     1921 gtagccctgg atctggtcgg cgtgaatgtc gacgctcaac agacgggtga ccccggcgtg
     1981 ggcaatgaga ttggcgacga gcttcgccga gatcggcacg cgcagcgagc gcggacggcg
     2041 gtcctggcgc gagtagccgt aatagggaac gaccgccgtg atccgccccg cagaggcgcg
     2101 catcagcgcg tcggcgatca agagcagttc catcaggttt tcgttgaccg gcgggcaggt
     2161 cggctgcacc acgaagacat cgcgcccgcg cacgttctcg aagatttcca cctgcacttc
     2221 gccgtcgctg aagcgtccga cgttgatctt tccgagcggc aactgcagat gcgaggcgat
     2281 gtccgccgcg agccccggat tggcccgccc ggcgaagacc atcatgtttc ccggtaccgt
     2341 ggaatctttc atgtcgcgaa tctcccggcc ttgcctcgag acagtggctg gggcgccagg
     2401 attcgaacct gggatgcggg gatcaaaacc ccgtgccttg ccgcttggct acgccccaac
     2461 atttccccgt tcgcgccgca ggcgctcgcg aagcggcgaa cgattgcggc cgcgggcgac
     2521 gatcgcccgc cacggccccg gcacttcggc cgcgagcgcc gccgccgcgt cacgcccgtc
     2581 gcgccaggtg aacacgcagc tcccggtgcc cgtaagccgc gccggtccgc gatcgcgcaa
     2641 ccaatcgaga gcgcgcgcga tctcggggta gcgccgacgc accaccggct cgcagtcgtt
     2701 gcgcgcctca tccagcgaga acgcagacat tttcgcgggc tcgctgtcgc gtgtcaattc
     2761 gggagcggcg aagattgcgg cggtcgaaac ggaaacggcc ggatgaatca cgacatacca
     2821 gggctcggga aactccgccg cgaagagccg ctcgcccacc ccgagcgcat gcgccgccgc
     2881 gccgcgcacg aacaccggca cgtcggcgcc aagttcgagc gcgagcgcag ccaagcgttc
     2941 gccgtcgagg ccaagccccg cgagcgcgtt gacggcaact agcgtggtcg cggcgtcgga
     3001 actggcgccg ccgaggccgc cgcctacggg gatgcgcttg tcgcggcgca catccatgcc
     3061 gagggatcgt ccggccgcgc actcgaggct gcggaccgcg cgcgcgacga gatcgtcctc
     3121 gactgcgacc cccggcaccg gatcgtgaag acggaattcg ccgtcatcgc gcggcgcgaa
     3181 ccacaggcga tcaccaaagt cgagaaactg gaaggccgtg gaaagttcgt ggtagccgtc
     3241 cgcgcgccgc ccggtgacgt gcagatacag gttgagtttg gcgggcgccg gccaggcgct
     3301 cagggcgtcg ggggaagctt ccagtcgttc acgacgagct tgatgtgtcc gcccggacgg
     3361 ctggccgcga ggcgcacagg aaggagaagc cccgccgcgg gcgcataggc ttcgtacgcg
     3421 acggtccagc cttgttgttg caattggatc acgagcccac ggctgtcgag gcggagttga
     3481 tacggacttc cgggcgccgg ctgcccggtc acccagtagc gcaggcttgc gaccgggatc
     3541 ggccagccga gacggcgctc gagcgccgtc tcgggcgtga cggtcgtctg cacatggccc
     3601 tgggaatcgc gcagatacat ttcgctcggc gtgccccaga ggctcacggc accgaggccg
     3661 aagggaccgg aaaagctgag cgacatctct tcgccctgct gtacccagga gacgttcgcc
     3721 gagccaccgt tgccgggagt gacgacaccg atgcgcccgt gaagtcgcca atcgtccaga
     3781 ttctgcaatc gtggcaggcg attttgccag gccgcgggat ttgccgggcc gcttggataa
     3841 agaagcgtcg tgcaaccgcc ggcgaggaag agccacagcc ccgcgagtgc ggggagaagc
     3901 tggcgcccga accactccgc tcgcatggct ccccgattgg cgccgacgat accggcattc
     3961 gcgccggtca tgagcggctc acggcgtcgc gcttttcacc atgcgttgct tcagccgcag
     4021 cagcgcggtg tcgtgcggcg ccttggcgag tgcgtcgtcg agcagcttgc cggcggccgc
     4081 gggattgccg agcgcgagat agagctgaac gagatgattg gcgacgtcgg aatcgcccgt
     4141 gagttcccag gcccgctcga ggtaaggcag cgcctttttg gcttcgccca tgcggaaaag
     4201 cacccagccc atgctgtcga gaattgcgcc gtcgtcaggc gcgagggtga gcgcccggac
     4261 gatgtagccg taggcctcgc ggtaccgcct gctgtgttcc gtgagggtgt agccgtaggc
     4321 gttgaggtag tccggattct cgggctcttc gccggcgagt tcatggagga gctggagcgc
     4381 ggtggcgccc tgaccgagct gatcggcgag cagagcctgc tgatagcgca gcggcggcac
     4441 gccgggatac cgtttcgccc catcggcgat gacggcgtag gcatcctgcg tccgtcccgc
     4501 ctgggagagc accgccgaat cgagtccgac ccaggtcgga tcgagcgccg ggaacgcgtc
     4561 gcgcagccgt ttgatgtagt ccagagccgg cgcgagtccc ttccactgcg caagcgcgag
     4621 gaccgcaccg gtctgggcgg aagcgaggaa gtcggagtcg gtgatgccct gataccagcc
     4681 gaaggcctcg gcccagtctt cttcggccgc ggccatgttg ccgaggaggt tgtagactcc
     4741 cgcctcgtcg ctgcccttct cgagcatccg cgtcgcgcgg ctgcgcgcgg cctgctcctc
     4801 gcccgccacc atgtcgacga tggcttcggc cagcgccacg tgggcgttgt tcggcgcgag
     4861 cgcccgcgcg tcgcccagca cctcgcgcgc gcgcccggtc tgattggcgc gggcgagcaa
     4921 cccagccgca aacagccgca acgtgacatc gtgcggagcg gaggcgagca tgcggtccag
     4981 catggcaatc gcctgctggc gttgcccgag gtcccagcgc gcctgtattt cgatcgtgcc
     5041 ggccgccgct ccgcctgtcg cgcccgcggc ctgcgcggcc cgcagcgcct cgcttgcgct
     5101 gccagccacg agcgcggcct gtgcataggc aagctgcagc tttgtgtcct cgggattggc
     5161 ttttgccagc ggttgcagga cggaaagcac aaaccagggg tcgatcggtc cggtgaggga
     5221 aagctgcacc gcatcggcgc cgccgagcgc gtaggcttcg gccagggcct cgcgtgccgc
     5281 ggcgatctgg ccctgatcga gcgcgatgcg cacctcgatc gcgaaaggct cggggtgttt
     5341 gggttcgagg ctgtgagcaa aatgcgctgc ctgggtcgcg agtgcgtaat cgcccgcctc
     5401 ggtggcgaaa agcgccgcga gaagcgcctg gttggcatcg tgcttgtcgc gggcctgatc
     5461 gagcagaacc atcgccgtaa ggcgcggtga atgaagctgc tgggccgtcg cgagagccgc
     5521 gccggcgctc gcaggcgccg cgacggaact cgcagccggt gctcgctgcg cggcgggagg
     5581 cgccgcgcag cctgcgagcg ccagtgcggc gactgccgcg gggacgagac cggaacgagc
     5641 catggaaggg tgaaatagaa gaagcctgac ggaaaactat actcgattgc gcaggcgttt
     5701 caccgccctg ctgcggtact atgaacgggg tttcgcgatc ttccgcccat gcttttcgtc
     5761 gtcggagtga gccacaagac ggccccgctc gcgctgcgcg agcgtctcgc cgtggctccg
     5821 gcacgggtgc ccgaactgct tgacgccatg ctcgaaggtg gcgatctgcg cgaagcggtt
     5881 ctgctttcga cctgcaatcg cagcgagttg tacgccgtca ccgaagccca ccgcaaggcc
     5941 gcggtactcg cctctttccg tgcgatcggc gactgcgacg aggagattct tcccggctgc
     6001 tatgtcgagg aagggcccgg cgcggcgcgc catctttttc gcgtggcggc gggtctcgac
     6061 agcatggtgc tcggcgaggc ccacatcctc gggcaggtaa gggcggccta tctcaccgcc
     6121 catcgcgcct ccgccgtcgg acccgaattg catcagcttt ttcagcacgt gctcgccgcc
     6181 accaagaaca tccgcagcga ctccagcctc gacggactgc gctcgcttcc ctacgctgcg
     6241 gccaagctcg cgcgcgaacg actcggcaaa tttggcgaac gccgcgccgt gctcatcggc
     6301 gccggaaaga ccatcgatgc gctcgcgttt caccttcgcg gcgaggtgag ccgactgcag
     6361 attgccaacc gatccccgca agcggcgcgc gctctcgccg cgcggcatgg agccgaggcc
     6421 atcctcttcg ccgatttgcc cgaggctctc gccgaagcgg atcttctcgc gagtgcgacg
     6481 acgagcgaaa cgccggtcgt gtcggccgac atgcttcaca aacgaacagg gacgcgctcg
     6541 ttggtggtcc tcgatctcgc ggtcccgcgg gacgtcgccg ccgacgtctc gctgcttgcc
     6601 ggcgtcgagg tcatcggact cgacgatctc gcggcggtga tagaagcgag cgcggaactg
     6661 cgctacgccg ccgcggagca ggccgagacc gcggtcgaag gggcgctcgc gggatggcag
     6721 cgggcgcggc gcatccgcga tgccgtgccc accatctgcg cgctgcgcgc cgaggcctcg
     6781 ctcgcccgcc gccagagcct cgccgcggcc cggcggatcg ccgccacacg cggcagcgat
     6841 gaggcgctcg agtatctcgc caccacgctc acgaaccgtc tcatccatgc ccccacggcg
     6901 cggctgcggg ccgcggcggc ggcggacgag gccacgatca tcgccacggc tcgcgaactc
     6961 ttcgggctgg ataacaaagg agcgcaggaa gacagcgcgg ccgcatgaac ccgctgctgc
     7021 tcgctcggct gcaggccctt ggcgaacgcg cccgcatcct cgcccgcgaa ctggaacaac
     7081 cgcagaccgc ggccgagccg cggcgattcg tggcgctttc gcgcgaatac tcccgcctcg
     7141 cttccgccga gcgcggcctt gccgaatacg agtcgctcga caaggcactc gccgaggcca
     7201 cccgcctcgc ggaagatggt gatt
//
LOCUS       DASUJG010000119         2934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1133807, whole genome shotgun sequence.
ACCESSION   DASUJG010000119 DASUJG010000000
VERSION     DASUJG010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133807"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..413)
                     /locus_tag="VFX38_02445"
                     /pseudo
     CDS             complement(195..413)
                     /locus_tag="VFX38_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650620.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            830..1150
                     /locus_tag="VFX38_02450"
     CDS             830..1150
                     /locus_tag="VFX38_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX5313752.1"
                     /translation="MDLLILKALSWGPMHGYGVSRWIRQVTEDTFDIKEGVLYPALHR
                     LEEKGWIDSEWGVSENNRQAKYYTLAPLGRRQLRRELSTWARFAEAVGKIVQATEKPA
                     WVQG"
     gene            1171..>2934
                     /locus_tag="VFX38_02455"
     CDS             1171..>2934
                     /locus_tag="VFX38_02455"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5313753.1"
                     /translation="MTRRIPIWRRYSTLFGRNIRRDIEDEIKFHVEARARELVDHGWP
                     PRAAEEEAHRLFGDRDSILMKCQQIDTRFEQRRKMLAYLADIAADVRYALRGFKRTPG
                     LTIVTLLTLAVGLGLTAAVFSVLNAVLLHPLPYAEPERIVQIVENVPAGEGFGGVAQR
                     RTAMSASDFIWWREHSTTLSHFAMMQRESRTLATPDGSVQLYGERVSPALFAIRGVQP
                     LLGRGLVADDERPDSDVVVLGEAAWRKYFNSAPDIVGRRIELDSRTFSVVGVMPPQFG
                     DQAFWVPLVVVATPNRTFLRPVEARLADGVSLETASAEANRLGLQLRGIEPEPGAKPR
                     FAVVRSVDELTAPVAPALRVLIVAVGVVLAIVCTNVANLLLVRGTRRQQEIAIRRSLG
                     ATRWRIARQVLTECLVLSVFAGLIAIAIAFAGVWLLKLTAAGYVSPRLRLSPSVLPRL
                     DEIAIDPPVLAFVALLSVVTGVLFGLLPALRLSKYGDRGHTSASQLSTLVRNSRLGHV
                     LAAVQLACAMALLIGAGLLVGSFLRITGIDPGFDPRGVLSFELVVPGESTAERKLEVA
                     ETLVTRLEADPRVTRAGFTDLP"
BASE COUNT          495 a          922 c          975 g          542 t
ORIGIN      
        1 agccggtacg ccgtgtcgag gagaaagtgc ttcaggttct cgcagttcac ttgctcgctc
       61 tgtagaaact gcgccatcga agccccggac atgatcggca agtaaaccgc cgaaacagca
      121 tccggtcaaa acacgacagt acgcccgcga tcttcggcgc accggaggcg gggccaagcc
      181 gcaggaaact tgccgagcca gcccgtgagc ggcagcgcga tcgcgaggct cacggcgaac
      241 gaagtgatga tccacgttcc ttggtctggg ctcaccgcga gatcgcccgc gatggtcggg
      301 atcgcgacgt tcgcgatcga cgtgtcgagc acgttcatga agatcgcgag cgacacgccg
      361 atcgtgaaga gcgcgagctt cgcgcccgtc atgggcggat agtgggtccc catgtcgttg
      421 actcccgtgg ttatcggtga gaggtgctta gttagaggtg accgcgccgg gcggggctga
      481 cggcggcccg catggggctg ggctctcttt tccgaacgtc tcgattcgcg cacatttggg
      541 gcattcggga tctcgcgccg aaaaaatggc tcgagtagtg gctctgcgag tcgttcgagt
      601 agcatcacgt taacgatgac gcgaccgctc acaatcgacc cgttagtgac gttaggttct
      661 tctgaaagaa ggttaggtta gcgccctgac acacaccacc gtgttgcgca agtcggaggc
      721 gttcgcagaa cccttgcatc actagggtac gctcgcttat gctcactcac ctagctatac
      781 aaggggagca acggtggctg gacggaaagt ttccatcctc aaaggagcga tggatctgct
      841 aatccttaaa gcgctcagct ggggccctat gcacggatac ggcgtctccc gctggattcg
      901 tcaggtgacg gaggacactt tcgacatcaa ggaaggcgtg ctgtacccgg ccctgcaccg
      961 cctagaggag aaaggctgga tcgattcgga gtggggcgta tcggagaaca atcgccaagc
     1021 aaagtactac actctcgcgc cgctcgggcg caggcaactc cggcgcgagc tctcaacatg
     1081 ggctcgcttc gcggaggccg ttggcaagat cgtccaggcg actgagaagc cggcctgggt
     1141 tcagggatag gctgctgagt cgcgcgcgcg atgactcgga gaatacccat ctggcgtcgt
     1201 tactcgactc ttttcgggcg caacatccgc cgcgacatag aggacgagat caagtttcac
     1261 gtcgaagccc gcgcgcgaga gctcgtggac cacgggtggc cgccgcgagc cgctgaagag
     1321 gaagcgcacc gcctctttgg ggatcgtgac tcgatcttga tgaagtgcca acagatcgac
     1381 acacgcttcg agcagagaag aaaaatgttg gcttacctcg ctgacatcgc agccgacgtc
     1441 cgctacgcgc tgcgcggttt caagcgcaca cccggcctca cgatagtgac cttgctcacg
     1501 ctcgcggtcg ggctcggctt aacggccgcg gtcttcagcg tcctgaacgc cgtcttgctc
     1561 cacccgctgc cgtatgccga gcccgaacgg atcgtgcaga tcgtcgagaa tgtgccggcc
     1621 ggcgaggggt tcggcggtgt cgcgcagcgc aggacggcga tgagcgcgag cgatttcatc
     1681 tggtggcgcg agcactccac gacgctttca cacttcgcga tgatgcagcg cgaaagccgg
     1741 acgctcgcga cccccgacgg cagcgtgcag ctctacggcg agcgcgtttc gccggcgctg
     1801 ttcgcgatac gcggcgtgca gccgctgctc gggcgcggcc tcgtggccga cgacgagcgc
     1861 ccggactccg acgtcgtcgt gctcggtgag gcggcatggc gcaagtattt caattccgct
     1921 cccgatatcg tcggtcgcag gatcgagctc gattcccgga ccttttccgt cgtgggcgtc
     1981 atgccgccgc agttcggcga ccaggcgttc tgggtaccgt tggtcgtcgt cgcgacgccc
     2041 aacaggacgt tcttacgccc cgtggaagct cggctcgccg acggagtttc gctcgagacg
     2101 gcgagtgccg aagccaacag gctcgggttg cagctgcgcg gcatcgaacc cgagcccggc
     2161 gccaagccgc gctttgcggt cgtgcgctcc gtcgacgagc tcacggcgcc cgtcgcgccg
     2221 gcgctgcgag tgctcatcgt ggccgtcggc gtcgtgctag cgatcgtgtg cacgaacgtg
     2281 gcgaatctct tgctcgtgcg cggcacgcgg cgccagcagg agattgcgat tcgccgctcg
     2341 ctgggcgcga cgcgctggcg catcgcgcga caggtcctca cggagtgtct cgtgctgtcc
     2401 gtgtttgcag gcttgatcgc gatcgcgatc gcgttcgcgg gcgtgtggct gctcaaatta
     2461 acggcggcgg gatacgtcag tccgcggctc cgtttgtcgc catcggtgct gccgcgactc
     2521 gatgagatcg cgatcgatcc gcccgtgctc gcgttcgtcg cgctgctgtc cgtcgtgacc
     2581 ggcgtgctgt tcgggctgct gccggcgctg cgcctttcga agtacggcga ccgcgggcac
     2641 acgtccgcat cgcagctctc gacgctcgtg cgcaactcgc ggctcggcca cgtgctcgcc
     2701 gcggtgcaac tggcgtgcgc gatggcgctg ctcatcggcg ccggcctgct cgtcgggagc
     2761 ttcctgagga tcacgggcat cgaccccggc ttcgatccgc gcggggtctt gagcttcgag
     2821 ctcgtcgtgc ccggcgaatc cacggcggag cgcaagctcg aagtcgcgga gacgctcgtg
     2881 acccgtctcg aggccgatcc gcgcgtgacg agggccgggt tcacggatct gccg
//
LOCUS       DASUJG010000120         4516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_32949, whole genome shotgun sequence.
ACCESSION   DASUJG010000120 DASUJG010000000
VERSION     DASUJG010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32949"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VFX38_02460"
     CDS             <1..189
                     /locus_tag="VFX38_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313754.1"
                     /translation="LDLHEVSRLWREAIVLGVEDSALNAALARERLVLLFVNAFRRPG
                     VGAFPAPGPRHLAATARR"
     gene            202..564
                     /locus_tag="VFX38_02465"
     CDS             202..564
                     /locus_tag="VFX38_02465"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX5313755.1"
                     /translation="MEVSHAPLHLDLRDRKRLARSYLSFVKGGGLFVPTETRYELGDE
                     AVLFVLLPGAAEERRIAGKVVWISPRGAASAHQQGIGVQLPSQEHGELHHQIEVLLAG
                     VAASPHPVSSFPKPERGA"
     gene            complement(609..1040)
                     /locus_tag="VFX38_02470"
     CDS             complement(609..1040)
                     /locus_tag="VFX38_02470"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX5313756.1"
                     /translation="MHFDLPPRDRHASVSTVAMPRDTNALGDIFGGWLMSHADIAGAI
                     LAYRRARGRVVTVAVNDFVFLAPVFVGDLVSFYSDLAHVGRTSLRVDVTMFAESDWGS
                     QDRVHRVATASMTYVHVDSQRRPLPVPDPQSPRVAPPAKKV"
     gene            complement(1040..3079)
                     /locus_tag="VFX38_02475"
     CDS             complement(1040..3079)
                     /locus_tag="VFX38_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX5313757.1"
                     /translation="MSESDYAHWRLETDEKGILWLGFDQAGTGTNTLGSAVLEELSVI
                     LDRLVVQPPHALIVHSRKDNGFAAGADVKEFTHIENEPQAYELIRRGQLVLNRLAALP
                     CPTVAMIHGFALGGGLELALACRYRIAADTTHTKLGFPEVKLGIHPGFGGTVRSVAVM
                     GVFAAMKLMLTGRSLGAGEAKARGLVDKVASAAELHERAINLALNPRRAPRASSLQRL
                     LSLPPARPLVARILRRQLAGRVREEQYPAPYAMVRLWRLHGGRLHAESFEAEAHSIAH
                     LMCGETARNLVRVFFLQNRLKALGRTPQFVPRQVQVIGAGTMGGDIAALCAARGIEVR
                     LQDREASFVEAAIARSRKFFERKSKDAHEIGEAAARLKMDLAGDDLPEAELVIEAITE
                     KRDAKLELFRDIEPRMAEDALLATNTSSIPLETLADALQRPERLVGLHFFNPVAKMPL
                     VEVVSMEETSAETRKRALAVVHALGKLPVPVRSAPGFLVNRILAPYLTAAFAAHDRGV
                     APEAIDRAAEDFGMPMGPLELADTVGLDVALAAAESLEGLVPVAPPQALRDLVAAGSL
                     GRKTGQGIYAWRNGKAQKQPKLAATASPLLQDLLILPFLNEAVRCLREKIVEDADLLD
                     AGMIFGTGFAPFRGGPLHYARRAGAQALAARLNELRADYGPLYSADPGWNELANH"
     gene            complement(4023..>4516)
                     /locus_tag="VFX38_02480"
     CDS             complement(4023..>4516)
                     /locus_tag="VFX38_02480"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE-like domain-containing protein"
                     /protein_id="HEX5313758.1"
                     /translation="THDQEEALEVADRIVVMKDGRIEQAGTPDEVYHHPVSEFVMSFV
                     GTVNRVEHRNWHDTVETNPGLPGSGVGTTTSNLIYVRPADLSVSLLPSPAPGVRAVVK
                     RINTAGPVVRLSLVAEDGQSLVAEMDHHRISELELHSGHTVQVSARRSRSFERLPSEP
                     APQ"
BASE COUNT          786 a         1406 c         1538 g          786 t
ORIGIN      
        1 cttgatttgc acgaggtgtc gcggctctgg cgcgaagcca tcgtgctcgg cgtcgaggac
       61 agcgctctca acgctgccct tgcgcgcgag cgtctcgttt tgctatttgt aaacgcgttt
      121 cgccgacccg gtgtcggcgc gttcccggcg ccgggtccgc ggcatctcgc cgcgacggcg
      181 cgccgctgag ggggaagtgc catggaagtt tcgcacgcgc cgctgcatct cgatcttcgc
      241 gatcgcaagc ggctcgcccg ttcgtatctg tcgttcgtga agggcggggg cctttttgtg
      301 cccaccgaga cccgctacga actcggcgac gaagccgtgc tgttcgtgct gcttccgggt
      361 gcggccgagg agagacgcat cgccggcaag gtggtgtgga tcagcccgcg cggcgcggcg
      421 agcgcccatc agcagggaat cggagtgcag ctcccctcgc aggagcacgg cgagcttcat
      481 catcagatcg aggtcctgct cgccggtgtc gccgcgtcac cgcacccggt gtcgtcattt
      541 ccgaaaccgg agcgcggcgc ctagcgtcga attccgcctg cgggtcggcc gccgacagcc
      601 gcggtggatc agactttctt tgcgggcggc gcgacgcggg gtgactgcgg atcgggcacc
      661 ggcagcggcc ggcgttgcga gtcgacgtgg acataggtca tcgaggccgt ggcgacccgg
      721 tgcacgcgat cctgcgagcc ccagtcgctc tcggcaaaca tggtcacgtc cacgcgcagc
      781 gaagttcgcc cgacgtgcgc gagatccgag tagaaactca cgagatcgcc gacgaatacc
      841 ggggcgagga atacgaagtc gttgacggcg acggtgacga cccgcccgcg cgcccgccga
      901 taggcgagaa tcgcgccggc gatgtcggcg tgcgacatga gccagccgcc gaagatgtcg
      961 ccgagcgcat tggtgtcgcg cggcatcgcg accgtggaca cggaggcgtg gcgatcgcgc
     1021 ggcggaaggt cgaagtgcat cagtggttcg cgagctcgtt ccagccgggg tccgcagagt
     1081 atagcgggcc gtagtccgcc cgcaattcgt tcagccgcgc cgcaagcgct tgcgcaccgg
     1141 ctcgccgcgc gtagtgaagc gggcctccgc gaaacggagc gaagccggtg ccgaagatca
     1201 tcccggcgtc gagaaggtcg gcatcctcga cgatcttctc acgcagacag cgcaccgctt
     1261 cgttgagaaa gggcagaatc agaagatcct gcagcagcgg actcgcggtg gccgcgagct
     1321 tcggctgttt ctgtgccttg ccgttacgcc aggcataaat cccctgaccc gtcttgcgcc
     1381 cgagacttcc ggccgcgacg agatcgcgca aggcttgcgg cggcgcgacc gggacgagtc
     1441 cctcgagcga ctccgccgcc gcgagggcga cgtcgagccc gacagtgtcg gcgagttcca
     1501 gggggcccat cggcatgccg aagtcctccg ccgcgcgatc gatcgcctcc ggtgcgacgc
     1561 cgcgatcgtg cgccgcgaaa gcggcggtca ggtagggcgc gagaatccga ttgacgagaa
     1621 atcccggagc gctgcgcacg ggaaccggca gcttgccgag cgcgtgaacc acggcaaggg
     1681 cgcgtttgcg ggtttcggcc gaggtttctt ccatgctcac gacttcgacc agcggcatct
     1741 tcgccaccgg attgaagaag tgcagcccca cgagccgctc cggtcgttgc agggcgtcgg
     1801 cgagcgtttc gagcggaatg ctcgaggtgt tggtcgcgag cagggcatcc tcggccatgc
     1861 gcggctcgat gtcgcggaaa agttcgagtt tggcgtcgcg tttttcggtg atcgcctcga
     1921 tgacgagttc ggcttccggc agatcgtcgc ccgcaaggtc catcttcagg cgagccgccg
     1981 cctcgccgat ttcgtgtgcg tctttgctct tgcgctcgaa aaatttccgt gatctcgcga
     2041 tggcggcttc gacgaagctc gcttcgcgat cctgcaggcg aacctcgatg cctcgtgcgg
     2101 cgcacaacgc ggcgatgtcg ccgcccatcg ttccggcgcc gatcacctgt acctgccgcg
     2161 gtacgaattg gggagtgcgc ccaagagcct tcaggcggtt ctggaggaaa aatacccgta
     2221 cgagattgcg tgccgtttct ccgcacatga ggtgcgcgat ggaatgcgct tcggcctcga
     2281 aactctccgc gtgcaggcgc ccgccgtgaa ggcgccacaa gcgcaccatt gcgtacggcg
     2341 ccgggtattg ttcttcgcga acgcgcccgg caagttgcct gcggagaatg cgggcaacga
     2401 gcgggcgcgc gggcggaaga ctgagcagcc gctgcagaga cgatgcccgc ggtgcgcggc
     2461 gcgggttgag ggcgaggttg atcgcacgtt catggagttc ggccgcgctc gcaaccttgt
     2521 cgacaagccc gcgcgccttc gcctcgcctg cgccgagcga acggccggtc aacatcaact
     2581 tcatggcggc aaaaacgccc atgacggcaa ccgaacgtac ggtgccgccg aagccgggat
     2641 gaatgccgag cttcacttcg ggaaagccca gtttcgtgtg cgtcgtatcg gctgcaatcc
     2701 ggtagcggca ggcgagcgcg agttcgaggc cgccgccgag ggcgaatccg tgaatcatcg
     2761 caacggtggg gcaggggagc gcggcaagac gattgagcac caattgcccg cgacgaatca
     2821 gctcgtaggc ctgcggctcg ttttcgatgt gcgtgaattc cttgacgtcc gcaccggccg
     2881 cgaagccgtt atccttgcgt gaatggacga tgagcgcatg cggcggttgc acgacgagcc
     2941 ggtcgagaat gacggacagt tcttccagca ccgcggagcc gagcgtattc gtgccggtgc
     3001 cggcttgatc gaacccgagc cagagaattc ccttctcgtc ggtttcgagt cgccaatgtg
     3061 cgtagtccga ttcactcatc gcgcccatcg ctccgcttgt gatggcccat cttagaagcc
     3121 ggcaagcggg catggcaagc gggtgaatcg cgccgggggc aaccggagca gaagggagcc
     3181 agtatcaaac tgcggcaagc gggggcgaaa gttccgagtc tggacttgtc atcgaatcgt
     3241 ttctcgcccg gagcctcgca tcgcgcacgg cgaaagcgcc gtcctgacag cgccggaaag
     3301 cgtgtgcagt cgcgcgcagc aggacgtccc cggaggcggg gcagggcagg cacagtcatc
     3361 tcgcgatcag tgcggacggg ctggccagta gccggtactt ttactcggcc aggtgattgg
     3421 ctgcggctct tcagacccga aaagttcgcg caagcgccat tgcgggtacc aggagcaata
     3481 agggatttgt cccggaccgg ggaaggcata cggacttcgg ggttccatcc ggacttggcg
     3541 ttgccattcg gcttgtgatt cgcctcgggc ctgcaacgca tacgcgagat cccggcggca
     3601 ttgcgtctcc tgcgcccgca agaccatccg gtccatcgaa cccggagacg gggcatcaaa
     3661 ctgcgcgtct cgaattgcgc tgggagacga gcgggtgtgc gaggcgtagg tgggaagagt
     3721 cgcggtcaca ctgaacggca ggccagcggc gaggacgggg gctgcccatc cggcgagtcc
     3781 catggcgagc gaaaagatac aaatttgcat tcggcagcac ctccttttct gtacgactgt
     3841 acaactgtcc ggtggcggac gcaagcgccg aatcaaaacg caggcgatgg tcggcgggga
     3901 tgttcgagcg gctccatggc gcatcaagct cgcgtccttg atgatccgca aacaatcgac
     3961 cgggacgtgc gcatacgccg gtggctgccg ccccaggcat ctgatggtgc gacggagagc
     4021 ctttattgcg gagccggttc cgacggcagg cgctcgaacg acctgctacg acgcgcggag
     4081 acctgcaccg tatgtccgct gtgcagctcg agttccgaaa tgcgatggtg gtccatctcg
     4141 gcgaccagcg attgtccgtc ttcggccaca agcgagagcc gcaccacggg gccggcagta
     4201 ttgattcgtt tcaccacggc gcggaccccc ggcgcagggg aggggagcag cgagacggag
     4261 aggtcggcgg gacgcacgta gatcaggttc gacgttgtcg ttccgacgcc cgagccggga
     4321 agccctggat tcgtttcaac ggtgtcgtgc cagtttcgat gctcgacccg gttgaccgtg
     4381 ccgacgaagg acatcacaaa ttcactgacc gggtgatgat aaacctcgtc tggtgtgccg
     4441 gcttgctcga tcctgccgtc cttcatgacc acgatgcgat cggccacttc gagcgcttct
     4501 tcctgatcgt gagtga
//
LOCUS       DASUJG010000121         2271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1378601, whole genome shotgun sequence.
ACCESSION   DASUJG010000121 DASUJG010000000
VERSION     DASUJG010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1378601"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="VFX38_02485"
     CDS             complement(<1..532)
                     /locus_tag="VFX38_02485"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5313759.1"
                     /translation="MANDSEFVAFWNRVLVPKFLRFQDILIEGFGKHSRIVFERGPVA
                     SPGERVLDVGCGFGDTAIALARQTGASGQVVGIDCCDAFLEIGRLDAAQAGVDHIDWL
                     EGDAETENFDAPFDLWFSRFGTNFFSTPVAALRHLREQLKPGGRAMMIVWQARERNRW
                     AALPREVVLQILPEPEA"
     gene            complement(677..2050)
                     /locus_tag="VFX38_02490"
     CDS             complement(677..2050)
                     /locus_tag="VFX38_02490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEX5313760.1"
                     /translation="MNRPEFSRRGLLPALMLPLLLVAAAAQATDLSESVTRAQHYDPT
                     LQASNYAAKAGKEGRNQATALYLPQISLIGEYQRVWSQTDVSVPPNFPFQVLAPLTSV
                     GNVYGYGVTLTQPLYSADVSVKAAQLRDQASLAEVQNHAAQQDLVSRVSEAYFNVIVG
                     YDTLRYVRAEKAAVAEQLASAKARFQAGRTNITDVQDAEARYDAIIAQEIQAENNLAE
                     WNARYQRAVGVTPDKLLRLPEDFQPTLPDPNDMNAWIQRGLAGNLDVQAKRIQQQLAN
                     KAIDLYTFASRPQLGLVASYSDTRQSGDLSFIVAPDRRQNAAIGLQLSIPIFSGGALV
                     SQLREARATSREATYDLQAAQADVTVQIREVFQSIQAGVKGIQALEQAAVTAKTSLEA
                     NQLALKVGVKSTQDVLNAEQTYYQTLEQLEQARYQYLMGMLSLQLLVGGLDDAAVARI
                     NQELHSS"
     gene            complement(2037..>2271)
                     /locus_tag="VFX38_02495"
     CDS             complement(2037..>2271)
                     /locus_tag="VFX38_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5313761.1"
                     /translation="HRFRPIVLTAGTAVLAMIPLTRSTFWAPMAWAIMGGLIAATVLT
                     LLYLPSLYALFYRVERKKKRADAGKGELSHEPA"
BASE COUNT          390 a          717 c          739 g          425 t
ORIGIN      
        1 gagcctcggg ttcgggcagg atttgcagca cgacctcgcg cggcagcgcc gcccagcgat
       61 tgcgctcgcg cgcctgccag acgatcatca tcgcgcgccc gcccggcttc aactgctcgc
      121 gcaggtggcg aagggccgcg acgggcgtgg aaaagaaatt cgtgccgaag cgcgaaaacc
      181 acaagtcgaa gggcgcatcg aaattctccg tttcggcatc gccttccagc cagtcgatgt
      241 ggtcgactcc cgcctgcgcg gcgtcaagac ggccaatctc gaggaaggcg tcacaacagt
      301 cgattccgac cacctgacca ctggcaccgg tctgacgtgc gagtgcgatc gcggtgtcgc
      361 cgaagccgca gccgacgtcg agcacgcgct cgcccgggct cgcgacgggg ccgcgctcga
      421 aaacgatgcg gctgtgtttg ccgaagcctt cgatcaggat gtcctggaag cgcagaaact
      481 tcggtacgag caccctgttc cagaacgcga cgaattcgga atcgttggcc atggttcttc
      541 cttgccgtgt cgcgcgaatt atagacctgc ctccccgctg ttcggttcac gtccggaatc
      601 gctgcatggc ggcggtgttt tgctgtttca acaaacgctt gcacgacttc tgcaagccat
      661 tcccgtggaa ggaacttcag gagctgtgca gttcctggtt gatccgggcg acggcggcat
      721 cgtcgagtcc gccgacgagg agttgcaaac tgagcatgcc catgaggtat tgatagcgcg
      781 cctgctcgag ttgttcgagc gtctggtagt aggtctgctc ggcattgagc acgtcctgcg
      841 tggatttcac cccgaccttg agcgcaagct ggttcgcctc gagcgaggtc ttcgcggtga
      901 cggctgcctg ttccagcgcc tgaattccct tgacgccggc ctgaatgctc tggaagacct
      961 cgcgtatctg aaccgtcacg tccgcctgcg ccgcctgcag gtcgtaggtg gcttcccggc
     1021 tggtcgcccg cgcctcgcgc aactgcgaga cgagcgcgcc gccgctgaag atcgggatgc
     1081 tgagctggag tccgatcgcc gcgttctggc gccgatcggg cgcgacgatg aaggagagat
     1141 cgccgctctg gcgcgtatcg gaatagctcg cgacgagtcc gagctgcggg cggctcgcga
     1201 aggtgtagag atcgatcgcc ttgttggcga gttgctgctg aatccgcttc gcctgcacgt
     1261 cgagattgcc ggcgaggccg cgctgaatcc aggcattcat gtcgttcgga tcgggcaggg
     1321 tcggttggaa gtcttccggc agccgcaaca acttgtccgg cgtcacgccg acggcgcgct
     1381 ggtagcgtgc gttccattcc gcaagattgt tctccgcctg tatttcctgg gcgatgatcg
     1441 catcgtagcg cgcctccgca tcctggacgt ccgtgatgtt ggtgcgcccc gcctgaaagc
     1501 gcgccttcgc ggaggcgagc tgctcggcga ccgcggcctt ttccgcgcgc acgtagcgca
     1561 gcgtgtcgta accgacgatg acgttgaaat acgcttcgct cacgcgcgag acgagatcct
     1621 gctgcgccgc atgattctgc acctcggcga ggctcgcctg atcgcgcaac tgcgccgcct
     1681 tcaccgatac gtcggcgctg tagagcggtt gcgtcagcgt gactccgtag ccatacacgt
     1741 tgcccacact ggtgagcggc gcgagcacct gaaacggaaa gttcggcggg acgctgacgt
     1801 cggtctgcga ccacacccgc tggtactcgc cgataagcga gatctgcggg agatagagcg
     1861 ccgtcgcctg attgcgtccc tccttgcccg ccttggcagc atagttcgag gcctgcagcg
     1921 tgggatcata gtgttgtgcg cgcgtcacgg actcgctcag atccgtcgcc tgtgcggcag
     1981 cggcgacaag caaaagcggc agcatgagcg ccgggagaag cccgcggcgg gaaaattcag
     2041 gccggttcat gcgacagctc gcccttccct gcgtcggccc gcttcttctt gcgttccacc
     2101 cggtagaaca gcgcgtagag cgaaggcaaa tagaggagcg tgagaacggt ggcggcaatc
     2161 agcccaccca tgatcgccca ggccatcggc gcccagaagg tcgagcgcgt gagcggaatc
     2221 atcgcgagca ccgcggtccc ggccgtgagt acgatcgggc ggaagcgatg c
//
LOCUS       DASUJG010000122         2174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_767910, whole genome shotgun sequence.
ACCESSION   DASUJG010000122 DASUJG010000000
VERSION     DASUJG010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767910"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VFX38_02500"
     CDS             complement(<1..343)
                     /locus_tag="VFX38_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313762.1"
                     /translation="MNDRFESLPLDLDAESFLALGHDMLELAAEWIAGEADDPLLQPI
                     GGVALANLLDEPPPERGMTNDALFVELRERVLRYARRNGHPRYFAYVMASADPVGALA
                     DLLVSAMNQNVT"
     gene            complement(440..1408)
                     /locus_tag="VFX38_02505"
     CDS             complement(440..1408)
                     /locus_tag="VFX38_02505"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX5313763.1"
                     /translation="MSRNAEPLRDEPGTPSENFAAARWKFAAAFAVVYVVWGSTYLFI
                     RIGVADIAPTVLAGLRFTAAGLALTAIGFARGEKPPRGRDWSTLAVLAVGLVVIGNGV
                     VTWAEQWVPSGEAAFIVASSALFTVLFGTLGPRGERLTLLVVAGLALGFLGTALMLWP
                     RIEGLRGPLWPALALVGSSCSWAAASMYARGVGVHTTAFMFSGLQMLAGGLILIAIAL
                     ASGGFARTHWSPSGIAALAYLAVFGSAIAYLAYNWLIQHARPAQLGTTAYVNPAVALL
                     LGWAVLGEVLPPIAFAGVGVMFVGVVLLTLRHRARVRPLVKPRTGA"
     gene            1619..>2174
                     /locus_tag="VFX38_02510"
     CDS             1619..>2174
                     /locus_tag="VFX38_02510"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX5313764.1"
                     /translation="MKRQGFTLFALAALAVSLAALPAFAALKAGATAPDFTAKASLAG
                     KDFTFSLKQALAKGPVVLYFYPAAYTGGCDIEAHTFATDAAKFAAAGATIIGVSADSI
                     QRLDSFSSDPNYCAGKFPVASDPDGKIAATYGLTLIPPQKGVTDVRGKAVTHGFIPRT
                     TFVIASDGKVVATLSSSADHLAPDQ"
BASE COUNT          405 a          734 c          723 g          312 t
ORIGIN      
        1 cggtgacgtt ctggttcatc gccgagacca gcagatcggc cagtgcgccg acgggatcgg
       61 ccgatgccat cacataggca aagtagcgcg gatgcccgtt gcgccgcgcg tagcgcaaga
      121 ccctttcgcg caactccacg aacaatgcat cgttcgtcat gccccgttcg ggcggcggct
      181 cgtccagcag attcgcgagc gcgacgccgc cgatcggttg cagcaaagga tcgtcggctt
      241 cgccggcgat ccattcggcg gcgagttcca gcatgtcgtg tcccagcgcg agaaacgact
      301 ccgcgtccag gtccagcggc agggattcga atctatcgtt catggcatgg ctcccgatgc
      361 ctcggcgcgg gcgggtacgg actccagagt atgccgcacg caagcccggt gcgctgcggt
      421 caaatcagtg cgggaacggt catgcgccgg ttcgaggctt gacgagcggg cgaacccggg
      481 cgcgatgacg cagggtcagg agtacgacgc cgacgaacat gacgccgacg ccggcgaagg
      541 cgatcggcgg cagcacctcg cccagcacgg cccagccgag cagaagcgcc acggccggat
      601 tgacgtaggc ggtcgtgccg agctgcgccg ggcgcgcgtg ctggatcagc cagttgtacg
      661 cgagatacgc aatggccgag ccgaacaccg cgagataggc gagcgcagcg atcccggaag
      721 ggctccagtg cgtgcgcgcg aagccgccgc ttgcgagcgc gatcgcgatg agaatgaggc
      781 cgccggcgag catctgcaat cccgagaaca tgaacgcggt cgtgtgcacc ccgacgccgc
      841 gcgcgtacat ggacgccgcg gcccaggaac agctcgaacc caccaaggcc agtgccggcc
      901 agagcgggcc gcgcaagccc tcgatccgcg gccacagcat cagggcggtg ccgaggaagc
      961 cgagcgcaag tcccgcgaca acgagcaagg taagacgctc gccgcgcggg ccgagggtgc
     1021 cgaacagcac ggtgaacagc gccgaacttg cgacgatgaa ggccgcctcg cccgaaggca
     1081 cccactgctc ggcccaggtc acgacgccat tgccgatgac gacgaggccg acggcgagca
     1141 cagcgagcgt ggaccagtcg cggccgcgcg gcggtttttc gccgcgggcg aatccgatcg
     1201 cggtcagtgc gaggccggcg gccgtgaagc gcagccccgc gagcacggtc ggcgcaatgt
     1261 cggcgacgcc gatgcggatg aagagatagg tcgaacccca gacgacatac accaccgcaa
     1321 aggccgcggc gaatttccag cgggccgccg caaagttttc cgaaggcgtt ccaggctcgt
     1381 cgcgaagggg ctccgcgttg cgggacacag tcgatccttg ttgcacacgc cgaatgcggc
     1441 gcgcgcattg tcgccgattt cgaaacaggt agcctcccga ggagcatcga gacttgcggc
     1501 aacctcgcca agcagcgccg ccaccgcgcc cgcggacgtg cttgaacgct gcggaccaat
     1561 ccccaaattg cggccgtccg gtgctacgat aaaaaacctc catcccgtgg ttgcggtcat
     1621 gaaaaggcag ggtttcactc tcttcgcgct cgccgcgctg gcagtctcgc tggcggcatt
     1681 gccggccttt gccgcgctca aggcgggtgc gacggcgccg gacttcaccg ccaaggccag
     1741 tctcgccggc aaggatttca cgttttcgct caagcaggcg ctcgccaagg gtccggtggt
     1801 gctgtacttc tatccggcgg cctataccgg cggctgtgac atcgaggcgc ataccttcgc
     1861 gaccgacgcg gccaagttcg ccgcggcggg cgcgaccatc atcggcgtgt cggcggacag
     1921 catccagcgc ctcgacagct tttcctccga tccgaattac tgcgccggaa agtttccggt
     1981 cgcctccgat cccgatggca agatcgcggc cacgtacggt ttgacgctca ttccgccgca
     2041 gaagggcgtc acggacgtgc gcggcaaagc ggtgacacac ggcttcattc cgcggacgac
     2101 cttcgtgatc gccagcgacg gcaaggtcgt cgccacgctt tcctcgagcg ccgatcacct
     2161 cgcgcccgat caac
//
LOCUS       DASUJG010000123         2016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1746927, whole genome shotgun sequence.
ACCESSION   DASUJG010000123 DASUJG010000000
VERSION     DASUJG010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1746927"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..1067
                     /gene="miaA"
                     /locus_tag="VFX38_02515"
     CDS             99..1067
                     /gene="miaA"
                     /locus_tag="VFX38_02515"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017024874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX5313765.1"
                     /translation="MNSGAGAIKADAKPAVLCISGPTGMGKSELALVLAEELPLEIVS
                     VDSAMIFRGMDIGTAKPNGAARARVPHHLIDIRDPAESYSAGEFARDARRIIGEIQDR
                     GRTALLVGGTLLYFGALLRGLSPLPGANRELREEIRREAAARGWESLHRELAKIDPAS
                     AERIHANDPQRLERAFELYRLTGLPPSRLYEQEKADAPFRFRRFALWPEDRARLYARL
                     GERFEAMMRAGLAGEVRALRARGDLTDALPALRVVGYRQLWGWLEGQYGEEEACRRAV
                     VATRRYAKRQLTWLRAEAQWQRFAGVEEAKTRILAGLRGRFSDALA"
     gene            1137..1427
                     /gene="hfq"
                     /locus_tag="VFX38_02520"
     CDS             1137..1427
                     /gene="hfq"
                     /locus_tag="VFX38_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007350635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEX5313766.1"
                     /translation="MSKSHNLQDPFLNLLRKDRVPVNVYLVNGIKLTGQIEAFDSFVI
                     VLRNNLSQMVYKHAVSTVQPARRIELPRQDDAQEHEADDADASAEEEDSPVS"
     gene            1447..>2016
                     /gene="hflX"
                     /locus_tag="VFX38_02525"
     CDS             1447..>2016
                     /gene="hflX"
                     /locus_tag="VFX38_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX5313767.1"
                     /translation="MNRSSERAVLVHRHPGTSGAAAGQAEFEALARAAGTEIVGTVVN
                     RRPPPDPHSFLGRGKVEAVRALAADGAADVVLVDEMLSPAQERNLEQAIGTRVLDRTE
                     LILDIFAQRARSFEGRLEVERAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQLESD
                     RRLIGKRIRQLNHQLEGVARRRAQGRALRR"
BASE COUNT          354 a          672 c          686 g          304 t
ORIGIN      
        1 atggagcgca caccgcgaag cgatcaatgc aatcacggcc gtccgacctg gatcgagttt
       61 tcgctggacg atctcgatcg gctttttcgc cgcggccgat gaacagcggc gccggggcga
      121 tcaaggccga tgcgaagccg gcggtgctgt gcatcagcgg gccgaccgga atggggaaaa
      181 gcgaactcgc gctcgtgctc gcggaagaac tgccgctcga aatcgtgagc gtggactcgg
      241 cgatgatctt ccgcggaatg gatatcggca cggcgaagcc gaacggcgcg gcgcgggcgc
      301 gcgtgccgca tcatctgatc gacattcgcg accctgccga gtcctacagc gccggcgagt
      361 tcgcccgcga tgcgcgccgc atcatcggcg aaattcaaga tcgcggacgc accgcgcttc
      421 tcgtcggcgg aacgctgctc tactttggcg cgctccttcg cgggctctcg ccgctgcccg
      481 gagcgaaccg cgaattgcgc gaggaaatcc gccgggaagc cgcggcgcgc ggctgggaat
      541 cgctgcatcg cgaactcgcg aagatcgatc ccgcaagcgc ggagcgcatt cacgcgaacg
      601 atccgcaacg cctcgagcgc gccttcgagc tgtatcgcct gacgggcctg ccgccgagcc
      661 gtctctacga gcaggagaag gccgatgcac cgtttcgatt ccggcgcttc gcgctctggc
      721 ccgaggatcg cgcccggctg tatgcgcgtc tcggcgaacg tttcgaggcg atgatgcgcg
      781 ccggcctcgc cggggaagtg cgggcgttgc gcgcgcgcgg cgatctcacg gacgcgcttc
      841 cggcgttgcg cgtcgtcggc tatcgccagc tctggggctg gctcgagggg caatacggcg
      901 aggaagaagc ctgccgccgc gccgtcgtcg cgacccggcg ctacgccaag cgccaactga
      961 cgtggttgcg tgccgaagcc caatggcagc gatttgcggg cgtggaggag gcgaaaacgc
     1021 ggatccttgc gggtctccgg gggaggtttt ccgacgcgct tgcgtgatag gataatcccc
     1081 gacgatgcgg cttcgtgccg cgtatcacaa accggaaaac atcggagaca acgacaatgt
     1141 ccaagagcca caacctgcag gatcccttcc tgaatctttt gcgcaaggac cgcgtaccgg
     1201 tcaacgtgta tctcgtcaac ggaatcaaac tgacggggca gatcgaagcc ttcgattcct
     1261 tcgtgatcgt gcttcgcaac aacctgagcc agatggtgta caagcacgcg gtttccaccg
     1321 tgcagccggc gcgccgcatc gaactgccgc gccaggacga cgctcaggaa cacgaagcgg
     1381 acgacgccga cgcgtccgcc gaagaagagg attcgccggt ctcgtgaggt cggaattacg
     1441 gccggcctga accgctcgag cgagcgcgcc gttctcgttc accgccaccc gggaacgagc
     1501 ggcgccgccg ccgggcaggc ggagttcgag gcgctcgcgc gggcggccgg aaccgagatc
     1561 gtcggaaccg tcgtcaaccg ccgcccgccg cccgatccgc acagctttct cgggcgcggg
     1621 aaggtcgagg cggtgcgcgc gctcgcggcc gatggcgcgg ccgacgtcgt gctcgtggac
     1681 gaaatgctga gcccggcgca ggaacgcaat ctcgagcagg ctatcggtac gcgggtgctc
     1741 gatcgcaccg aactcattct cgacatcttt gcccagcggg cgcgttcctt cgaagggcgg
     1801 ctcgaggtcg agcgcgcaca actgcgccat ctcgcgaccc gcctcgtgcg cggctggacg
     1861 catctcgaac gccagaaggg cggcatcggc cttcgcgggc cgggcgaaac ccagctcgaa
     1921 agcgaccggc gcctgatcgg caagcgcatc cgccagttga accatcagct cgaaggcgtc
     1981 gcccgacgcc gcgcgcaggg ccgggcgctg cgccgc
//
LOCUS       DASUJG010000124         2684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1747028, whole genome shotgun sequence.
ACCESSION   DASUJG010000124 DASUJG010000000
VERSION     DASUJG010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1747028"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VFX38_02530"
     CDS             complement(<1..520)
                     /locus_tag="VFX38_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019186067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX5313768.1"
                     /translation="MNSGHDLVRALRREQVARRPVWLMRQAGRYLPEYRELRERAGSF
                     LALLKTPELAAEVTPQPLRRFDLDAAIVFADILTIPDAMGLGLEFTPGEGPRFRRPVA
                     RLADARGLTVPDPESDLSYVLATLRTTAQALDGRLPLIGFAGGPWTLAAYMLEGGGGA
                     REFPRARAAAQAQ"
     gene            complement(517..1227)
                     /gene="pyrF"
                     /locus_tag="VFX38_02535"
     CDS             complement(517..1227)
                     /gene="pyrF"
                     /locus_tag="VFX38_02535"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006913025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX5313769.1"
                     /translation="MNPVPEARERLVFALDVPTIAEAEALVAHLGNVVVFYKLGLELF
                     TAGDAAGLVYRLKAAGKRVFVDLKLFDVPATVARAVARLAALEVDFLTVHGNQAMMEA
                     AAGAKGASRVLAVTALTSLDEGDLADLGFRCELEALVASRARRALEAGLDGVVASGRE
                     AKMLRSQLGPRLLVVTPGIRPLANREEDDQKRVMSPAAAIRAGADYLVVGRPIRDADD
                     PRAAAERIVGEIAAALAP"
     gene            complement(1259..2341)
                     /gene="aroB"
                     /locus_tag="VFX38_02540"
     CDS             complement(1259..2341)
                     /gene="aroB"
                     /locus_tag="VFX38_02540"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009146938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX5313770.1"
                     /translation="MNAQGIEIPGIAYPVWIGDDAGVREEYAALLVGRDVLIVSDGNV
                     AGHHLAPLREASGGAARVETLVLPAGEGEKTLGGLERILDALVVGGFHRDALVVALGG
                     GVVSDLAGFAAACYQRGVDWIAVATTLLAQVDAAIGGKTAVNHRAGKNLVGAFHDPVA
                     VWADPARLATLPEREYLAGFGEVVKYGLGFDAEFFAWLETNRSALLHRDTTALAHAIR
                     RCARCKLELVAADPRERGRRILLNLGHSFGHALEAVLGYGTWLHGEAVAAGLVAAAEL
                     SCARGRLPAEVPPRLRRLLQTFRLPTEIPGEADEASLLAALALDKKIAAGRLRFVGLE
                     ALGRAAVWEDVGAGEITRVLRRLRSG"
     gene            complement(2338..>2684)
                     /locus_tag="VFX38_02545"
     CDS             complement(2338..>2684)
                     /locus_tag="VFX38_02545"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX5313771.1"
                     /translation="REHESRILASALARESVVIATGGGVIEEERNRSALNSAIVIWLR
                     APAAVLASRLQGQVARPLLGNSPREDLERFARRRESLYAAVANFRVDAEDSPPEVLRR
                     ILAELRRLRIVS"
BASE COUNT          469 a          912 c          915 g          388 t
ORIGIN      
        1 gctgagcctg cgcggccgcg cgcgcccggg ggaattcgcg cgctccgcca ccgccttcga
       61 gcatgtaggc ggcgagcgtc caggggccgc ccgcaaaacc gatgagcggc aggcgtccgt
      121 cgagcgcttg cgccgtcgtg cgaagcgttg ccagcacgta ggaaagatcc gattcagggt
      181 cgggaacggt caacccccgc gcatccgcga gacgcgcgac ggggcggcga aaccgcggtc
      241 cctcgccggg cgtgaactcg agcccgaggc ccatcgcatc cgggatggtg aggatgtcgg
      301 cgaacacgat cgccgcgtcg agatcgaatc ggcgcagcgg ttgcggcgtg acctcggcgg
      361 cgagttcggg cgtcttcaga agcgcaagaa agcttccggc ccgttcgcgc aattcgcgat
      421 attccggaag ataacgcccg gcctggcgca tcagccatac gggccggcgc gcaacttgct
      481 cgcggcgaag cgcacggacg agatcgtgcc cggagttcat ggggcaagcg ccgcggcgat
      541 ctcgccgacg atgcgctcgg ccgccgcgcg cggatcgtcg gcatcgcgaa tcgggcgtcc
      601 gacgacgagg taatcggcac cggcgcggat cgcggccgcc ggactcatga cccgcttctg
      661 gtcgtcttct tcgcggttgg cgaggggacg gatgccgggc gtgacgacga ggagacgcgg
      721 gccgagttgc gagcgcagca tctttgcttc gcgacccgag gcgacgaccc catcgagtcc
      781 cgcctcgagc gcacggcgcg cgcgtgaggc gacgagcgcc tccagctcgc agcgaaatcc
      841 gagatcggcg agatcgcctt cgtcgagact ggtgagcgcg gtgaccgcga gcacgcggct
      901 cgcgcccttc gcgcccgccg ccgcttccat catcgcctga ttgccgtgca ccgtaaggaa
      961 atcgacctct agcgccgcga gcctggcgac ggcgcgggcg accgtcgccg gcacatcgaa
     1021 gagcttgagg tccacgaaaa cgcgcttgcc ggcggccttg agccggtaca cgagtcccgc
     1081 cgcgtcgccc gcagtgaaca attcgaggcc gagcttgtag aagacgacaa cattgccgag
     1141 atgggcgacc agcgcctcgg cttcggcaat cgtgggcaca tcgagcgcaa agacgagtcg
     1201 ttcgcgcgct tccggaacag gattcatgct ggctcgacgg tcgggtgcat gcgagattct
     1261 agccgctgcg aaggcggcga aggacgcgcg tgatttctcc tgctccgaca tcctcccaga
     1321 ccgccgcccg gccgagcgcc tcgagtccga cgaagcgcag gcggccggcg gcgatcttct
     1381 tgtcgagcgc gagggcggcg agcagcgaag cctcgtccgc ctcccccgga atctccgtcg
     1441 gcaggcggaa ggtctgcagg agccgacgaa gccgcggcgg aacttcggcg ggaagccttc
     1501 cgcgcgcgca ggacaactcg gccgcggcga cgagacccgc ggcgaccgcc tcgccgtgca
     1561 accaggttcc gtagccgagc accgcttcga gcgcatgtcc gaaactgtgg ccgagattga
     1621 gcaggatgcg ccggccgcgc tcccgcggat cggcggcgac gagttcgagc ttgcagcgcg
     1681 cgcagcggcg aatcgcgtga gcgagcgcag tcgtgtcacg atgaagcagc gcagagcggt
     1741 tcgtttcgag ccaggcaaaa aattccgcgt cgaagccgag cccgtatttc accacttcgc
     1801 cgaaacccgc gagatattcg cgctccggaa gggtcgcgag ccgggcggga tcggcccaga
     1861 ccgcgacggg atcgtgaaag gcgccgacga gattttttcc cgcgcgatga ttgaccgccg
     1921 tcttcccgcc aatggcggcg tccacctgcg cgagcagcgt cgtcgccact gcgatccaat
     1981 cgacgccgcg ctgataacag gcggcggcga agccagcgag gtctgacacg actccgccgc
     2041 cgagcgcaac gacgagagca tcgcgatgga agccgccgac gaccagtgca tcgaggatgc
     2101 gttcgagtcc gccaagtgtt ttttcgccct cgcccgccgg cagaacaagc gtttcgaccc
     2161 gcgccgcccc tccgctcgct tcccgcagcg gcgcgagatg atggccggcg acgttgccgt
     2221 cgctcacgat caggacgtcg cggccgacga gaagcgccgc atactcctcg cgcacgccgg
     2281 catcgtcacc gatccacacg ggataggcaa tacccggaat ttcgatgcct tgcgcgttca
     2341 cgaaacgatc cgaagccggc gcagctccgc cagaatgcgg cgcaatactt cgggcggcga
     2401 gtcttctgcg tccacccgga aattcgcaac cgcggcatac agcgactcgc gccgtcgcgc
     2461 gaagcgctcg agatcttctc ggggactgtt cccgagaagg gggcgcgcga cttgtccttg
     2521 caggcgtgat gccagcacgg cggccggtgc tcgcagccag atcacgatcg cggaattcag
     2581 cgcacttcgg tttcgctctt cttcgatgac tcccccgccg gtcgcgatga cgacagactc
     2641 gcgggcaagc gcggaggcga gaatgcgact ctcgtgttcg cgaa
//
LOCUS       DASUJG010000125         2816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_891339, whole genome shotgun sequence.
ACCESSION   DASUJG010000125 DASUJG010000000
VERSION     DASUJG010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891339"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /gene="dnaJ"
                     /locus_tag="VFX38_02550"
     CDS             complement(<1..866)
                     /gene="dnaJ"
                     /locus_tag="VFX38_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010133521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX5313772.1"
                     /translation="MSERDYYETLGIARDASEAEVKQAYRRKAMKYHPDRNPGDKSAG
                     EHFKEINEAYEVLGDAAKRRAYDQFGHAGVRGGAASGGFGGFGGIDPGDIFGDLFGNI
                     FGGSARGSRRGADLQYRLAVELEQISRGEEVVVEIPRQTRCETCAGSGAAPGSKPRVC
                     ATCGGRGQVRIQQGFFTLQQTCPDCRGRGEVIGEPCPDCRGSGRVRRISRLKVRIPPG
                     VEDGTAIRLSGQGEAGGPGATDGDLYVEIRIRAHPIFRREAGNLACEVPVSFPQAALG
                     GEVAIPTLEGEGR"
     gene            complement(863..1444)
                     /locus_tag="VFX38_02555"
     CDS             complement(863..1444)
                     /locus_tag="VFX38_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEX5313773.1"
                     /translation="MNTSRMEAFSDGVIAIIITIMVLGLMEVRTEGAAWTSLKPVLPA
                     FLSYVLSFVYVGIYWNNHHHMLRATRRVNGRILWANLHLLFWLSLFPFVTAWAGENRF
                     ATVPSALYGVVLLMAAIAYVILQRSIIAHNGRDSLLAQATGKDLKGKASMFLYVAGIG
                     LSFANNWAGNALYVLVAIVWLVPDRRIESRITE"
     gene            complement(1441..1890)
                     /locus_tag="VFX38_02560"
     CDS             complement(1441..1890)
                     /locus_tag="VFX38_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008439325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX5313774.1"
                     /translation="MSQEGGEGCRLKRPRHDLKAWQEAMELARKVYTLVRDFPAEERY
                     GLTAQIRRSAISVPSNIAEGCARGSAREFLQFPYIARGSLSELETQLRLAHDFGFCDA
                     REVLSKIEALFALFGGLIKSQRRHTDALQAAKPPTDRSRKRPTCLTQ"
     gene            complement(1919..>2816)
                     /locus_tag="VFX38_02565"
     CDS             complement(1919..>2816)
                     /locus_tag="VFX38_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX5313775.1"
                     /translation="KVQDAVKTFFGREPRKDVNPDEAVAIGAAIQGGVLGGEVKDVLL
                     LDVTPLSLGIETLGGVMTKLIEKNTTIPTKATQVFSTAADNQPAVTVHVLQGEREMAG
                     ANKSLGRFDLSDIPPAPRGMPQIEVTFDIDANGILHVSAKDKATGRENRIEIKASSGL
                     SDDEVQRMIKDAEAHAEEDKKARELVEARNQADALIHASEKSLKELGDKVEAGERAEV
                     ESAISDLREALKGDDRQRIETKTEALGTASAKIAQRIYQAQQQQSAAGGGESGGEAPR
                     GNGDEGVVDAEFEEVKDDKDGK"
BASE COUNT          482 a          957 c          833 g          544 t
ORIGIN      
        1 cggccctcgc cttcgagcgt gggaatcgcc acctcgccgc cgagcgccgc ctgcggaaag
       61 gaaacgggca cttcgcaggc aagattgccc gcctcccgtc gaaagatcgg atgcgcgcgg
      121 atgcgaatct ccacgtagag atcgccatct gtcgcgccgg gtccgccggc ctccccttgt
      181 ccggaaaggc ggatcgcggt gccgtcctcg acgccgggag gaatgcgcac cttgagacgc
      241 gagatgcgcc gcacccggcc gctaccgcga caatccgggc agggctcgcc gatgacctcg
      301 ccgcgtccgc ggcaatcggg acaggtctgc tgcagcgtga agaagccctg ctgaatgcgc
      361 acctgaccgc ggccgccgca ggtcgcgcac acccgcggct tgctgcccgg agccgcgccg
      421 cttccggcgc aggtttcgca acgcgtctgg cgcggaatct cgacgacgac ctcctcaccg
      481 cgggaaatct gttcgagttc caccgcgagg cgatactgga gatccgcgcc gcggcgggaa
      541 ccgcgcgcac taccgccgaa aatgttgccg aaaagatcgc cgaagatgtc gccgggatcg
      601 attccgccga aaccaccgaa gccgccgctg gccgcgcccc cgcgcacgcc ggcatgaccg
      661 aactgatcgt aggcgcggcg cttggccgcg tcgccgagca cctcgtaggc ttcgttgatc
      721 tccttgaaat gttcgcccgc gctcttgtcg ccgggattgc gatcgggatg gtatttcatc
      781 gccttgcgcc gataggcctg cttcacctcg gcctccgaag catcgcgggc aatccccagg
      841 gtttcgtaat aatcgcgttc gctcattccg tgatccttga ttcgattctg cgatccggca
      901 ccagccacac gatcgccacc agcacataca gcgcattgcc ggcccagttg ttcgcaaagg
      961 acagtccgat gcccgcgacg tacaggaaca tcgacgcctt gcccttgagg tctttcccgg
     1021 tcgcctgtgc gagcagtgag tcgcgcccgt tatgcgcaat gatgctgcgc tgcaatatga
     1081 cataggcgat agccgccatc agcagcacca cgccgtagag tgcgctcggc accgtggcga
     1141 atcggttctc gcccgcccag gcggtgacga aggggaaaag cgacaaccag aagagcagat
     1201 gcagattcgc ccagaggatg cgcccgttca cgcggcgtgt cgcccgcagc atgtgatgat
     1261 gattattcca gtagattccg acgtagacga aactgagcac gtagctcaga aaggccggaa
     1321 gcacgggctt cagcgaagtc catgcggcgc cctcggtgcg cacctccatc aacccgagca
     1381 ccatgatggt gatgatgatg gcgatgacgc cgtcgctgaa tgcctccatc cgactggtat
     1441 tcactgcgtc aagcaagtcg gccgcttacg cgacctgtca gtcggcggct tcgccgcctg
     1501 taaagcgtca gtatgccgac gctgcgactt gattaggccg ccgaacaatg cgaataacgc
     1561 ttctattttc gacaagacct cgcgtgcatc gcaaaaccca aaatcgtgag cgagtcgcaa
     1621 ctgcgtttcg agttcggaca gcgaacctcg ggcgatgtac gggaactgca aaaattcgcg
     1681 ggcacttccg cgtgcacagc cttcggcaat gttggatggc acgctgatgg ctgaacggcg
     1741 aatttgcgca gtcagcccat agcgttcttc cgcagggaag tcgcgaacaa gtgtataaac
     1801 tttgcgcgcc aactccatcg cctcttgcca agctttcaga tcgtgacgcg gcctcttcaa
     1861 ccgacagccc tccccgcctt cctggctcac ttactcacta acgtacttac gctcgtactt
     1921 acttaccgtc cttgtcgtcc ttgacttcct cgaattcggc gtccaccacg ccctcgtcgc
     1981 cgttgccgcg cggcgcttcg ccgccgctct cgccgccgcc tgcagccgac tgctgctgtt
     2041 gcgcctggta aatgcgctgc gcgatcttcg ccgaggcggt gccgagcgct tcggtcttcg
     2101 tttcgatgcg ttgcctgtcg tcgcccttga gcgcctcgcg aagatcggag atcgcggact
     2161 cgacctcggc gcgctctccc gcctcgacct tgtcgccaag ttccttgagc gatttctcgc
     2221 tcgcgtgaat cagggcgtcc gcctgattgc gcgcctcgac gagttcccgt gccttcttgt
     2281 cttcctcggc gtgcgcctcc gcgtccttga tcatgcgctg cacttcgtcg tcggagaggc
     2341 cggaggaggc cttgatctcg atgcggtttt cccgccccgt cgccttgtcc ttggccgaaa
     2401 cgtgaagaat gccgttggcg tcgatgtcga aggtcacctc gatctgcggc atgccccgcg
     2461 gcgccggcgg aatgtcgctg agatcgaagc gcccgagcga cttgttcgcg cccgccatct
     2521 cgcgctcgcc ctgaagcacg tgcacggtga cggccggctg attgtcggcg gcggtcgaga
     2581 acacctgcgt cgccttcgtc ggaatcgtgg tgttcttctc gatcaacttc gtcatgacgc
     2641 cgcccagcgt ttcgatgccg agcgaaagcg gcgtgacatc gaggagcagc acgtctttca
     2701 cctcgccgcc gagcacgccg ccctggatcg cggcgccgat tgcgacggcc tcgtccgggt
     2761 tcacgtcctt gcgcggctcg cgcccgaaga aggtcttcac cgcatcctgc accttg
//
LOCUS       DASUJG010000126         2524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1257414, whole genome shotgun sequence.
ACCESSION   DASUJG010000126 DASUJG010000000
VERSION     DASUJG010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257414"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1836)
                     /locus_tag="VFX38_02570"
     CDS             complement(<1..1836)
                     /locus_tag="VFX38_02570"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEX5313776.1"
                     /translation="MEILYGARALPDFRLERMRGRLSAAIPALDLQDARFFYLFSGDL
                     PAGGARKGLESFFEEGVAGEPAAAETLRVVVVPRFGTVSPWSSKASEILAGCGLGGVG
                     RVERGVGYALRGPVPAPVDEAWSRLAAVLSDRMVEVMLPSIAEGAALFRLRDPEPDRS
                     IALTAQGEAALLEADERLGLALGAPEREYLLAAYGALGRDPSETELMMFAQVNSEHCR
                     HKIFRGNWTLDGEAQGRSPFDCIRESYRAHPEGILSAYRDNAAVIAGADGRSWSRDPA
                     SGIYTAGDAALDVAIKVETHNHPTAVAPFAGAATGAGGEIRDEAATGRGGSPKAGVTG
                     FAVSDLRLAAVARSWEEAVGTPAGLASALRIMLEAPIGAASFNNEYGRPAIAGFFRSF
                     CVADPNSGPGRFRGYHKPVMLAGGIGSIRRPLIEKKRFGAGAKIVVLGGPAFAIGLGG
                     GAASSRAGGRNDELDFASVQRGNPEMQRRAQEVIEAATALGESNPILAIHDVGAGGLA
                     NAVPELLHEAGLGGRLDLALVPSADSAMSALALWCNESQERYVLALAPEEEAGFAELA
                     RRERCPWAVIGEATDDGILRVIDGRAGATVVDLAMQTLLGDLPPLA"
     gene            complement(1844..>2524)
                     /locus_tag="VFX38_02575"
     CDS             complement(1844..>2524)
                     /locus_tag="VFX38_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5313777.1"
                     /translation="VDCGFSTAEIERRLARLAVAPDRIDAILVTHEHSDHVAGVARFA
                     AAHRIPVRASAGTAASPALAGCALPFDSHAPFAIGDLEVTPLIVPHDACEPTQFVLGD
                     GARRIAVLTDAGHVTPYLLAALREIEALILEANHDETLLAAGPYPASLKRRVGGDYGH
                     LSNAQAADLLGALAPGKLQQLALAHLSEQNNRPELARAAASTALGCTPDWIAIADQDG
                     GLGWREVN"
BASE COUNT          436 a          879 c          826 g          383 t
ORIGIN      
        1 ggcaagcggc ggcagatcgc cgagcagagt ctgcatcgcc agatcgacga cggtcgcgcc
       61 ggcccgcccg tcgatcacgc gcaagatccc gtcgtcggtc gcttccccga tcacggccca
      121 gggacaacgt tcgcgccggg caagctcggc aaaccccgcc tcctcttccg gcgcgagcgc
      181 gagcacgtag cgctcctgcg attcattgca ccagagcgcg agcgcgctca tcgcgctgtc
      241 ggccgaaggt accagggcga gatcgagccg cccgccgagc cccgcctcat ggagcaactc
      301 gggcacagca ttggcaagcc cgcccgcgcc gacgtcatga atcgcgagaa tcggattgga
      361 ttccccgagc gcggttgcag cttcgatcac ttcctgcgcc cggcgctgca tctccggatt
      421 gccgcgctgc acggaggcga aatcgagttc gtcgttccgc ccgcccgcgc gcgaggaagc
      481 cgcgcctccg ccgagcccga ttgcgaacgc cggtccgccg agcaccacga tcttcgcccc
      541 ggcaccgaaa cgcttctttt cgatcagcgg ccggcgaatg ctgccgatgc cgccggcgag
      601 catcacgggt ttgtgatagc cgcgaaagcg acccggcccc gaattcggat ccgccacgca
      661 gaaactgcgg aaaaatccgg cgatcgcggg gcggccgtac tcattgttga aggaggcggc
      721 gccgatcggc gcctcgagca tgatccgaag cgcgctcgcg agccccgccg gcgtacccac
      781 ggcctcctcc caggatcgcg caactgccgc gagacgaaga tccgaaacgg cgaagcccgt
      841 aactcccgcc ttcgggctgc cgccccgtcc ggtcgccgcc tcgtcgcgaa tctcgccgcc
      901 ggcgccggtt gcggctcccg caaacggagc gacggcagtc gggtgattat gtgtttccac
      961 cttgatcgcg acatcgagtg cggcgtcgcc ggcggtgtag attccgcttg cgggatcgcg
     1021 gctccagctt cgtccgtccg cgccggcgat cacggcggca ttgtcgcgat aggccgagag
     1081 aattccttcc ggatgcgcgc ggtaactctc gcggatgcag tcgaacggcg aacggccttg
     1141 cgcctcccca tccagggtcc aattcccgcg aaaaatcttg tgccggcaat gttccgaatt
     1201 cacctgggcg aacatcatca attcggtttc gctcggatcg cgcccgagcg cgccgtaggc
     1261 ggcgagaagg tattcgcgct cgggcgcccc aagcgcaagg ccgagacgtt cgtcggcctc
     1321 gagcaaggcc gcctctccct gcgccgtgag cgcgatgctg cgatcgggct ccggatcgcg
     1381 caggcgaaaa agcgctgcgc cctccgcgat ggaaggaagc atcacttcga ccatccggtc
     1441 ggaaagaacc gcggcgagtc gcgaccaggc ctcgtcgaca ggcgcgggaa cggggccgcg
     1501 cagcgcataa cccactcccc gttcgactct ccccacccca ccgaggccgc aaccggcgag
     1561 gatttcgctt gccttgctgg accagggcga aaccgttccg aatcgcggca cgactacgac
     1621 gcgcaatgtt tcggctgccg caggctcgcc cgcgacgcct tcttcgaaaa agctctcgag
     1681 acctttgcgg gcgccgccgg caggcagatc gccggagaaa agatagaaga agcgcgcatc
     1741 ctgcaagtcg agcgcgggaa tcgctgcgga caatcggccg cgcatccgct cgaggcgaaa
     1801 atcgggcaag gcgcgtgcgc cgtaaagaat ctccacgacc ttctcagttc acctcgcgcc
     1861 agccgaggcc gccgtcctgg tctgcgatcg cgatccagtc gggcgtgcag ccgagcgcgg
     1921 tcgacgccgc ggcgcgggca agttccgggc ggttgttctg ctccgagaga tgtgcgagcg
     1981 cgagttgctg cagttttccg ggagcgaggg cgccgagaag gtccgcggcc tgcgcgttgc
     2041 tgagatgtcc atagtctccg ccgacgcggc gcttcaagga ggcgggatag ggaccggccg
     2101 cgagcagtgt ttcgtcgtgg ttcgcctcga gaatcagcgc ctcgatttcc cggagcgccg
     2161 cgagcagata tggagtgacg tgccccgcgt cggtcagcac cgcaatccgc cgcgcgccgt
     2221 cgccgagcac gaattgcgtc ggttcgcacg cgtcgtgcgg cacgatgagc ggagttacct
     2281 cgagatcgcc gatcgcgaaa ggcgcatggc tgtcgaaggg caacgcgcag cccgcaagtg
     2341 ccggcgaggc cgcggtgccc gcactcgccc gcacggggat gcgatgcgcg gcggcaaaac
     2401 gtgcgactcc ggcgacatga tcggaatgtt catgcgtcac cagaatcgcg tcgatcctgt
     2461 ccggcgcgac cgcgagacgc gccagccgtc gttcgatttc cgcagtcgaa aagccgcagt
     2521 ccac
//
LOCUS       DASUJG010000127         2325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_402339, whole genome shotgun sequence.
ACCESSION   DASUJG010000127 DASUJG010000000
VERSION     DASUJG010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402339"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..797)
                     /locus_tag="VFX38_02580"
     CDS             complement(93..797)
                     /locus_tag="VFX38_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313778.1"
                     /translation="MSTDSEKLRDLLLHRLPEAEADSLRERVLLDEDFAAALRDEEYA
                     LLDDHAAGRLTAAERAAVERHLLVTAQDRLRLRVAQALARRQAKSASRPRWFGWPMAL
                     VASLAVIGIVAGVWWGIVVPRRGVTPVVTVALLAEVSRGAGQAPLILTLPARGGVRIQ
                     AQVLDPAPGARYTLWVRDTAGHRLYRSRPLALRHTGGYAYVQAVVPAATLGTGARLVR
                     VAGPGYAFQWSVQLRH"
     gene            complement(859..1479)
                     /locus_tag="VFX38_02585"
     CDS             complement(859..1479)
                     /locus_tag="VFX38_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313779.1"
                     /translation="MGRLRAAGGLAITAETQSAFHSLLARLGAEGKVEVEYERLRRRL
                     IVYFRLSFPVQAETLADTALDRLARRLDEGVAVENVAAYTLGIARLLVLEAQAQALRE
                     QKAVRLETAASQDDDAEEDGEDEELERQRTAAALETCLKSLNTAGAELILSYYRADGS
                     DRIRVRQKLAAGLGVSVHALRNRALRLRAALEDCVRRRLQVAGVTE"
     gene            complement(1443..>2325)
                     /locus_tag="VFX38_02590"
     CDS             complement(1443..>2325)
                     /locus_tag="VFX38_02590"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX5313780.1"
                     /translation="ARHRNVVVVADGELQRLPFGVLRMGNAQTMLGSDHDLVYLPSVS
                     TLRLIRDVQRPTSATGALAIFADPVFRADDPRLPAMRNRQGSAKDLAAASGDIGLANL
                     PPLPYSLREAEAIRARAGKARVWLASGFAANRAAVLHADWSRYAIVHFASHSLLDMQR
                     PELSGIVLSLYQSDGRAVDGFLRVSDLYDLDMPVNLVVLGSCDSAVGPNPGSEGVFSL
                     ARALFYAGARRTLASLWPVDDRASAQFMSLFYTALLTDHEAAPAALRQARMTMAADPQ
                     YRQPYYWAGYVLQGDWQ"
BASE COUNT          407 a          806 c          766 g          346 t
ORIGIN      
        1 cttgttcatg gaactctcct gagcttcggt caaaggtatc cgtcaagtag tgagcggaac
       61 ggccgaaacg tcacgcggga gaatgcaaac gctcagtggc ggagctgcac gctccactgg
      121 aaggcgtagc cggggccggc aacgcggacc agtcgcgcgc cggtgccgag ggtggccgcg
      181 ggcacgacgg cttgtacgta ggcatagccg cccgtgtgcc gcagggcgag cggccgggag
      241 cgatacagcc ggtgaccggc ggtatcgcgg acccacagcg tgtagcgggc gccgggagcg
      301 ggatcgagca cctgggcctg gatccgcacg ccgcccctgg cgggcagggt cagaatgagc
      361 ggagcctggc ccgctccgcg cgagacctcg gcgagcaggg ccaccgtcac caccggcgtg
      421 actccgcggc gcggcacgac gattccccac cacaccccgg ccacgatccc gatcaccgcg
      481 aggctcgcga caagcgccat cggccatccg aaccatcgcg gccgggatgc cgattttgcc
      541 tgtcgccgcg ccaacgcctg cgccacgcgc aatcgcaggc ggtcctgcgc cgtcacgagc
      601 aggtgccgct cgaccgcggc gcgttccgcc gcagtcagac ggcccgcggc atgatcgtcc
      661 agcagcgcgt attcctcgtc gcgcagcgcc gcggcgaaat cttcgtcgag cagcacgcgc
      721 tcgcgcaaag aatcggcctc cgcctccggc aggcggtgca acagcaaatc gcgcagcttc
      781 tccgaatccg tactcaagca ccggcctccg cttcatgctt cgggagttgg ctccctcacc
      841 cttctgtgtg ccgaaacgtc attccgtcac gcccgccacc tgcaagcgcc gccgcacgca
      901 gtcttccagc gccgcccgca agcgcaaggc gcggttgcgc agggcgtgca ccgaaacgcc
      961 cagacctgcg gcgagttttt gccgtacgcg gatgcggtcg ctcccgtccg cgcggtagta
     1021 cgagaggatc agttcggcgc cggcggtgtt cagggacttc agacaggttt ccagcgccgc
     1081 cgcggtacgt tggcgctcca actcctcgtc ttccccatcc tcctccgcat cgtcgtcttg
     1141 cgaggccgcg gtttcgagcc ggaccgcctt ttgctcgcgc agggcttgcg cctgcgcttc
     1201 cagcaccagc aagcgcgcga tcccgagcgt gtaggcggcg acgttctcca ccgccacccc
     1261 ctcgtcgagt cggcgagcga gacggtcgag cgccgtgtcg gcgagcgttt cggcctgcac
     1321 cggaaagctc agacggaagt agacgatgag acgccggcgc agtcgctcat actcgacttc
     1381 gactttgccc tccgcgccga gacgcgcaag cagggagtga aaggcggatt gtgtttcggc
     1441 ggttattgcc agtccccctg cagcacgtag ccggcccaat aatagggctg gcggtactgc
     1501 gggtccgcgg ccatcgtcat gcgtgcctgc cgcaacgccg ccggagcggc ttcgtgatcg
     1561 gtcaacaacg ccgtatagaa caagctcatg aattgcgcgc tggcacgatc gtccaccggc
     1621 cacaggctcg cgagcgtgcg ccgcgccccg gcatagaaca aggcccgcgc gaggctgaac
     1681 acgccctcgg agccgggatt cggtccgacg gcggaatcgc agctcccgag caccacgaga
     1741 ttcaccggca tgtcgagatc gtaaagatcg gagacgcgca gaaaaccgtc cacggcacgg
     1801 ccgtcggatt gatacagcga gagcacgatg ccggaaagct ccgggcgctg catgtcgagc
     1861 aggctgtggc tcgcgaaatg cacgatcgca tagcggctcc agtcggcatg aagaacggcg
     1921 gcgcgattcg ccgcgaagcc gctcgcgagc cacacccgcg ccttgccggc gcgcgcacgg
     1981 atcgcctcgg cttcgcgcag ggaatacggc aacggcggca aattcgcgag gccgatgtcg
     2041 ccgcttgcgg cggcgagatc cttcgccgat ccttgccggt ttcgcatcgc gggcagacgg
     2101 ggatcgtcgg cacggaacac cggatcggcg aagatcgcga gcgcccccgt tgcggaagtc
     2161 ggccgctgta cgtcacggat cagacgcagc gtacttacgg aaggcagata cacgagatcg
     2221 tgatcgctgc ccagcatggt ttgcgcattt cccatgcgca gcacgccgaa gggcaggcgt
     2281 tgcaactcgc cgtccgcgac cacgacgaca ttgcgatgac gagcg
//
LOCUS       DASUJG010000128         3656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1747141, whole genome shotgun sequence.
ACCESSION   DASUJG010000128 DASUJG010000000
VERSION     DASUJG010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1747141"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..2434)
                     /gene="nuoG"
                     /locus_tag="VFX38_02595"
     CDS             complement(65..2434)
                     /gene="nuoG"
                     /locus_tag="VFX38_02595"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEX5313781.1"
                     /translation="MSDERVKLTVDGKTLEAPKGEMLMRATDEAGIYIPRFCYHKNLR
                     VVANCRMCLVEVKGAPKPMPACATPVADGMEVFTGSGRAIDAQRSTMEFLLINHPLDC
                     PVCDQGGECELQDLSWGYGRGVSRFAERKRVVRDKSIGPLVHTAMTRCIHCTRCIRVL
                     EEVGGRQEMGATERGEHLKIGTWIERSIDSELSGNIIDVCPVGALNDKPFKMRARGWE
                     LLQHATVSAHDCAGSNIYGHSLRGRFLRAVPRENESINDCWISDRDRWSHTGLAAADR
                     VKRPLVRRDGALVETDWEEALAFAAESLRGSGEGLGTLVAPGASLEEMYLAQKLTRGL
                     GSRNVDARLRQSDFRDDEGDPRFPSLGSSFAALEKSDAILLVGSHLTKEVPIAGYRVR
                     RAAQQGAAVMALNPRRYDFAMPLTGEHVVHPDRLVAGLAAFARAVAKAKGRALPDFLE
                     AFADADDTDLAANAGRFAEAERPRILLGHLAHQHPRFADLRRLAVALGALADAPVGYL
                     SEGANLAGAYLAGAVPHRGPGGVPVAAGLDVRAMLAEPRKVYLTIGTEPEKDCWDGAT
                     ARAALAAARVVALTSFLAPAMREYADCVLPLAAFGETAGSYVNAAGDVQRFDAAAVPA
                     GDSRQGWKILRALGAELVAASGFGFTALAEVRAEVMGAIGHVHHDASYGGQWRPAPGE
                     DELSALRAVTEVGLYAGEPLLRRAAPLQATADANGNSRAWLHPDDAAERGLESGDRLR
                     IAAGASVCEAELGLDSGVVHGSLWVPPGSGLALWESAVTLAALPQAAEA"
     gene            complement(2439..>3656)
                     /gene="nuoF"
                     /locus_tag="VFX38_02600"
     CDS             complement(2439..>3656)
                     /gene="nuoF"
                     /locus_tag="VFX38_02600"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX5313782.1"
                     /translation="EKILRERPERSAVIEEVKKSGLRGRGGAGFPTGVKWSFMPKSPP
                     SPDKHLVCNSDESEPGTCKDRDILRFNPHALVEGMAIASYAMGVELAYNYMRGEFMHE
                     PRIRFEAALGEAREAGLLGPNIRGTGINVRIENVLGAGAYIAGEETALLDSLEGKHAF
                     PRFKPPFPAAKGAWDRPTTVNNTETLASVPAILRNGADWFMKLGKDEKNGGPKVFSVT
                     GHVARPGNFEIPLGLPFADLLELAGGMRGGKKLKAVIPGGSSVPVLPAEAMMACTMDY
                     TSIQQAGSMLGSGAVIVMDEDTCMVKMLECISRFYYSESCGQCTPCREGTGWLYRSVK
                     RIEAGAGRPEDLDRLVDLAHHIEGHTICAFGDAAAMPVYSFIQHFRDEFEYHIREHRC
                     LVGPGAARTVKAA"
BASE COUNT          610 a         1254 c         1198 g          594 t
ORIGIN      
        1 ccagccggga acctgcatcc agacgccgga gacaaattgc gtgaactgat gcacggcgcg
       61 cgccttacgc ctcggccgcc tgcggcaggg ccgcgagcgt caccgccgat tcccacagcg
      121 ccagccccga gcccggcgga acccacaggc tgccgtgcac cacaccggaa tccaggccca
      181 gttcggcctc gcagacgctc gcgcctgcgg cgatacgcag gcgatcgccg gattcgaggc
      241 cgcgctcggc ggcatcgtcc ggatggagcc aggcgcggga attcccgttc gcgtccgccg
      301 tcgcctgcag cggtgcggcg cggcgcagga gcggctcgcc ggcatacaat ccgacttcgg
      361 tcacggcgcg aagcgccgac aattcatctt cgccgggcgc gggacgccac tgaccgccat
      421 agcttgcgtc gtggtgcacg tgtccgatgg cgcccatgac ctcggcgcgc acttccgcga
      481 gcgcagtgaa gccgaagccg ctcgcggcga cgagttcggc cccgagggcg cggaggatct
      541 tccagccctg gcgcgagtcg cccgcgggaa cggcggccgc atcgaaacgc tgcacgtctc
      601 cggccgcgtt gacgtaactt ccggccgtct cgccgaaggc cgcgagcggc agcacgcaat
      661 ccgcgtactc gcgcatcgcc ggcgcgagaa acgacgtcag cgccacgact ctcgccgcag
      721 cgagcgccgc gcgcgccgtg gcgccgtccc agcaatcctt ttccggctcg gtgccgatcg
      781 tgagatagac cttgcgcggc tcggcgagca tcgcgcgaac gtcgagtccg gcggcgacgg
      841 ggacgccgcc gggaccgcga tgcggcaccg cgccggcgag ataggcgccg gcaagattcg
      901 cgccttcgct gagataaccg acgggcgcgt cggcgagcgc gccaagagcg acggcgaggc
      961 ggcgaagatc ggcgaagcgc ggatgctgat gcgcgagatg cccgagaagg attcgcggcc
     1021 gctcggcttc ggcgaagcgt ccggcattcg ccgcgagatc ggtatcgtcg gcatccgcaa
     1081 aggcttcgag aaaatcgggg agcgcgcgtc ccttcgcctt cgcgacggcg cgcgcgaatg
     1141 ccgcaagccc ggccaccagc cgatccggat gaacaacgtg ttccccggta agcggcatcg
     1201 cgaagtcgta gcggcgcgga ttcagggcca tgacggccgc gccctgctgc gcggcgcggc
     1261 gcacgcggta gccggcgatc ggcacctcct tcgtgaggtg cgagccgacg agcagaatgg
     1321 cgtcggactt ctcgagcgcc gcgaacgaac ttccgagcga agggaagcgc ggatcgccct
     1381 cgtcgtcgcg aaagtccgac tgccgcaggc gcgcatcgac gttgcgggaa ccgaggccgc
     1441 gggtgagctt ctgcgcgaga tacatttctt ccagcgatgc tcccggcgcg acgagcgttc
     1501 cgagtccctc gcccgatccg cgcagcgact cggcggcgaa ggcaagcgcc tcctcccaat
     1561 cggtctcgac gagcgcgccg tcgcgacgta cgagcgggcg cttcacccga tcggcggccg
     1621 cgagtccggt atgactccag cggtcgcgat cggagatcca gcaatcgttg atcgattcgt
     1681 tttcccgcgg caccgcgcgc aggaatctcc cgcgcagcga atgtccatag atattggagc
     1741 cggcgcaatc gtgcgccgac accgtcgcgt gctgaagaag ctcccagccg cgtgcgcgca
     1801 tcttgaaggg cttgtcgttg agcgccccca cggggcagac gtcgatgatg ttgcccgaaa
     1861 gttccgaatc gatgctgcgc tcgatccagg taccgatttt caaatgctcg ccgcgctcgg
     1921 tcgcgcccat ttcctggcgc ccgccgactt cctcgagcac gcggatacag cgcgtgcagt
     1981 gaatgcagcg cgtcatcgcc gtgtgcacga gcggcccgat ggatttgtcc ctcaccacgc
     2041 gtttgcgttc ggcgaaacgc gaaactccac gcccgtaccc ccaggaaaga tcctgcagct
     2101 cgcactcgcc gccctggtcg cataccggac aatcgagcgg atggttgatg agcaggaatt
     2161 ccatcgtcga gcgctgcgca tcgatcgccc gcccggaacc cgtgaacact tccattccgt
     2221 cggcgacagg cgtggcgcag gccggcatcg gcttcggcgc ccccttcact tcgacgagac
     2281 acatgcggca gttggcgacg acccgcagat tcttgtggta gcagaagcgc ggaatgtaga
     2341 ttcccgcctc gtcggtggca cgcatcagca tctcgccctt cggcgcttcg agcgtcttgc
     2401 cgtccaccgt gagtttgacg cgctcgtcgc tcatcgtctc aggccgcctt taccgtgcgc
     2461 gcggcgccgg gaccgacgag acaacggtgc tcgcgaatgt gatattcgaa ctcgtcgcgg
     2521 aagtgctgga tgaaactgta gaccggcatc gccgcggcgt cgccgaaggc gcagatcgtg
     2581 tgtccctcga tatgatgcgc gagatccacc agccgatcga gatcctcggg gcgtcccgct
     2641 cccgcttcga tacgcttgac cgagcgataa agccagcccg tgccctcgcg gcagggcgtg
     2701 cactgcccgc aggattcgga ataatagaag cgcgagatgc attcgagcat cttcaccatg
     2761 caggtgtcct cgtccatcac gatgaccgcg cccgagccca gcatcgagcc cgcttgctgg
     2821 atgctggtgt aatccatcgt gcaggccatc atcgcttccg ccggaagcac cggcaccgag
     2881 gagccgcccg gaatgaccgc cttgagcttc ttgccgccgc gcattccgcc cgcgagttcg
     2941 agaaggtccg caaacggcag tcccagcggg atttcgaagt tgccgggacg cgcgacgtgg
     3001 cccgtgacgg aaaagacctt cgggccgccg ttcttttcgt ccttgccgag tttcatgaac
     3061 cagtcggcgc cgttgcgcag gatcgcgggt accgaggcga gcgtttcggt gttgttgacc
     3121 gtcgtcgggc ggtcccaggc gcccttggcg gccggaaacg gcggcttgaa ccggggaaag
     3181 gcgtgcttgc cctcgagcga atcgagaagc gcggtctctt cgcccgcgat gtaggcgccg
     3241 gcaccgagca cgttttcgat gcggacgttg attcccgtgc cgcggatatt cgggccgagc
     3301 agccccgcct cgcgcgcctc gccgagcgcg gcctcgaaac gtatgcgcgg ctcgtgcatg
     3361 aattctccgc gcatgtagtt gtaggcgagt tcgaccccca tcgcgtagct cgcgatcgcc
     3421 atgccttcga ccagcgcatg gggattgaag cgcagaatgt cgcgatcctt gcaggtaccg
     3481 ggctcgcttt cgtccgaatt gcataccaga tgcttgtctg gcgagggtgg cgacttcggc
     3541 atgaaactcc acttgacgcc ggtcggaaag ccagcgccgc cgcggccgcg aagccccgac
     3601 ttcttcacct cctcgattac cgcactgcgc tcggggcgtt cccggagaat cttctc
//
LOCUS       DASUJG010000129         2474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1258088, whole genome shotgun sequence.
ACCESSION   DASUJG010000129 DASUJG010000000
VERSION     DASUJG010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258088"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /gene="zapE"
                     /locus_tag="VFX38_02605"
     CDS             complement(<1..553)
                     /gene="zapE"
                     /locus_tag="VFX38_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HEX5313783.1"
                     /translation="MTLVNPPALTRIENAYREALAGGRIEADAAQANAVGALARLERE
                     LTVSRFPWRDRPARGVYLHGPVGRGKTWLMDLFFRNVPIENKLRRHFHAFMQEEIHAA
                     LRALAGQRDPLPRIARDLARRARLLCFDELLVEDIADAMLLGTLFEALFAEDVALVTT
                     ANVPPAGLYRDGLQRERFLPAIAA"
     gene            652..2167
                     /gene="glpK"
                     /locus_tag="VFX38_02610"
                     /pseudo
     CDS             652..2167
                     /gene="glpK"
                     /locus_tag="VFX38_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019592653.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
BASE COUNT          424 a          795 c          861 g          394 t
ORIGIN      
        1 gcgccgcgat cgcggggaga aagcgttcgc gctgcaatcc gtcgcgatag agtcccgcgg
       61 gcggcacgtt ggcggtcgtg accagcgcga cgtcttcggc gaacagtgcc tcgaagagcg
      121 tcccgagcag catcgcatcg gcgatgtcct cgaccagcag ctcatcgaag cagaggagac
      181 gcgcccgacg cgcgagatcg cgggcgatcc gcggcagagg atcgcgttgt ccggcaaggg
      241 cacgcagggc tgcatggatt tcttcttgca tgaaggcgtg aaagtgacgt cgcaacttgt
      301 tttcgatcgg aacgttgcgg aagaagagat ccatgagcca ggtcttgccg cgaccgaccg
      361 ggccatggag atacactccg cgcgccggtc tgtctcgcca cgggaaccgt gacaccgtga
      421 gttcccgctc caatcgcgcc agggcgccga cggcattcgc ctgtgcggca tcggcttcga
      481 tccgcccgcc cgcaagcgct tcgcgatacg cgttttctat tctcgtgagt gcgggcggat
      541 tcaccagagt cacggcaggc ggcgcggaag cgacagtgta gcgaagcggg cgggcttgcc
      601 ttgttcgcgg cccgggggta ggctgcttcg aaccgaaacg gctgcggccg catgccccat
      661 cttcttgcgc tcgaccaggg gacgacgagc agccgcgcgt tcgtcttcga tgcatcgttt
      721 cgggtcgttg cgagtgcgcg ctctgcgctc gcggcgaatt acccgcgccc ggcctgggtg
      781 gaacaggatc cgaccgcgat tctggaaacg cagcgcgagg ccgccgaaaa cgcgcttgcg
      841 caggccggaa tcggcgccgg ggacctgatc gcgctcggga tcacgaatca gcgcgaaacg
      901 acaattctct gggagcgcgc gacgggcagg ccgatcgcgc cggcgatcgt gtggcaggac
      961 cggcgcacgg cgtccgtctg cgcgcggttg cgcgagcgcg gcatcgaaac gatggtgcgc
     1021 gcgaaaaccg gccttccgct cgatccatat ttttcggcga ccaagatcgc atggctgctc
     1081 gcgcatgtga acggtgcgcg cgagcgggcg ctgcgcggcg aactcgcctt cggcacgccg
     1141 gacagttggc tcgtatggaa cctcagcggc ggaaaaattc atgcgaccga cgtgaccaat
     1201 gcttcgcgca cgctgctctt cgacatccac aaactcgaat gggacgagga actgctcgaa
     1261 ctcttcgagg tgccgcgcgc gctgctgccg gaagttcgcg cctcgaccgg cggtttcggg
     1321 acggcacggc tcgcgggcgg cgagatcgca atcgcgggag tcgcgggcga ccagcatgcg
     1381 gcgctgctcg gacagtcctg catcgcgcgc ggtgacatca agaacaccta cggcaccggc
     1441 gccttcgtcg tcgcgaacac cggaccggag ccggtcgcaa gtgaccaact tctttccacg
     1501 cttgcctggc aactgcgcgg cgaaaaaccg gtgtatgcgc tcgaaggctc gatcttcgcc
     1561 gccggcgccg cggtgcagtg gttgcgcgat ggcctcggcc tgatcgccac ggcggaagaa
     1621 tccgaaaccc tcgcagcgca ggtacctgac agcggcggcg tgagcttcgt gcccgcgctc
     1681 gccggactcg gcgcgccgtt ctgggatccc gaggcccgcg gcacgattct cggcctcacg
     1741 cgcggaacga aagccgcgca cctcgcgcgc gactctggag gcgatcgcct tgcgcacgcg
     1801 cgacgtgatc gaagccatgc gcgccggcgg catcgaagtg caggaactgc gggtggacgg
     1861 cggcgcggcg tgcaacgatc tcctcatgca gatccaaagc gatttcctcg gcgttccggt
     1921 gcggcgtccc gtcgttaccg agacgacggc gctcggtgcc gcgttgctcg ccggcatcgg
     1981 cgccggtctc gtcgcgccgg acgaactgca aagcatctgg cgcgaggagc gccgcttcga
     2041 accgacgatg tccggcgcgg agagtgaccg ccgctatcgc gaatggcaac gtgcctgcga
     2101 ccgcgcccgc cactgggcgc gcgaaagcaa cgatattccc tctcccgagg ggagagggtc
     2161 agggtgaggg gtttccgtgc ggacatgaaa cggacgagtg agcgcagata cgcaggaacc
     2221 gcaggccggg acaaccgcgc atcctgcgcg cgtttccggc aattcgtgta ccgccggcaa
     2281 ggatcgttga tcctatgccc gcctgcagga actatattcc ctcccccgag gggagagggt
     2341 tagggtgagg ggttttcgtg ggctaatgaa acggaggagc ttcgcttgaa cgacggcatg
     2401 gatcgcgcgg cgaatctcgc gcggctggag cgcgaggcat tcgacgtcct cgtcatcggc
     2461 ggcggcgcga ccgg
//
LOCUS       DASUJG010000130         9242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_36364, whole genome shotgun sequence.
ACCESSION   DASUJG010000130 DASUJG010000000
VERSION     DASUJG010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9242
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36364"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1757)
                     /locus_tag="VFX38_02615"
     CDS             complement(<1..1757)
                     /locus_tag="VFX38_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313784.1"
                     /translation="MRKRHRIRGIVLAAITFAFALPAAAGSLSPRLQTLADSWRQAPV
                     ATLARLTAASGVQTQIFAPRVSATGRVEVYIRFRPGEMPAAAALATLGAENVVFSPAL
                     GIVQAWVPIGRLRETSQLAAVVRVDLPAYGRAKVATLPDAMPRAGGVPPGLRIDREGI
                     SGLDVQPLVNAGMDGSGLRVGVISVGVDGLSESQNAGYLPNNVWVAPGLAGEGAEGTA
                     MLEEVHAMAPGAALGFCGPATTVDFVTCLDDLDNEFNADVIVDDLGFFAAFLFNQPDG
                     DALVNAVGDFIAAHPQINLATAAGNDALDYFQHAYLADTHPNRISLHPDYTPGPGQAG
                     GRSYQSAMNFGAAVHQHSDATEPTLIAPTSLDPAIALNAFLTWNDPAGGPYDDVDLFL
                     VKANGTVVASSTYDQTQGPDPGIGDGEYIYYQNKTGSNQTLYLAALCFQCAHPILIKL
                     NGFLDGAGTFQYATKGGIFGRAGLAGELAVGAAQVVGGPPAIADMEPFSDSGPYTYGD
                     WQSGIQTRPKPDLVGIDGVTVSGAGGFPSPFYGTSAAAPNVASVISLLRGAFPDGASN
                     AAGWAQIVDSNANPDALNK"
     gene            complement(1809..2342)
                     /locus_tag="VFX38_02620"
     CDS             complement(1809..2342)
                     /locus_tag="VFX38_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313785.1"
                     /translation="MPGLRMVFSLRMLSVVTGAALLAGCGAPPRSGAGHASSDATVVS
                     PTAAVASNTSARNAASADAAAENKIAPMLLAVANRVRATANGTEAAALSDSRVHVSRR
                     REIEIYVHVREVTPPMKAALAGAGARDIRASAPLRTYQAWASPVAIERVAKLPGVLRI
                     SAPAYAFPRSGAAFGGP"
     gene            2524..3063
                     /gene="ppa"
                     /locus_tag="VFX38_02625"
     CDS             2524..3063
                     /gene="ppa"
                     /locus_tag="VFX38_02625"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX5313786.1"
                     /translation="MDFTKLGPGKNPPDDLNVIIEIPAQAAPVKYELDKGTGLLAVDR
                     FLSTSMVYPANYGLIPATLCDDGDPLDVLVVTPLPLIHGALIRVRPVDLLEMSDEKGG
                     DAKLVAVPVTGVYAGYGEAQSVDDLPGGLKHQITHFFENYKAFEPGKWVKLEGWGGLE
                     RARKEVLDCIERCRGPAAG"
     gene            complement(3134..3907)
                     /locus_tag="VFX38_02630"
     CDS             complement(3134..3907)
                     /locus_tag="VFX38_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5313787.1"
                     /translation="MSGLLACRGLGVRRGRRVLVEDFDFSLAAGEIVAVVGPNGAGKS
                     SLLATLAGLLPPESGGVFMDGRPLARLSPRRIASRLGFLPQDSQDPYPATVLETALIG
                     RHPHIGFWQWESAADVAKARAALRRVGLAPAAARAIATLSGGERRRLAIATLLTQAPQ
                     IYLLDEPLETLDFAYQARLLRLLRGLARLAGAGIMLSLHDLSLAARHADRAVLLDGRG
                     GAAVGSAREVLRAGPIAAAYGCRVRVLEMEGLPFYVVGG"
     gene            complement(3904..4905)
                     /locus_tag="VFX38_02635"
     CDS             complement(3904..4905)
                     /locus_tag="VFX38_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX5313788.1"
                     /translation="MAELRLPALRRSGLILALLAVAAAAALVGSLFVGSVALGPGEVL
                     AAIGGRGSRLADALVLSLRLPRSLAAFATGGLLGLAGALMQVLLRNPLADPYILGVSG
                     GAAAGALIAILVGLGTAALTGLAFAGALATTLVVFVLAHGRGSWAPTRLLLTGVVIAA
                     GWGAAIALLLSLSPDATLRSMLFWLMGDLSFRSDWQLPLAVLAAALVLSLPCARPLNV
                     LARGELQARALGVSVRPLTIGLYIAASVLTAVAVVEAGTIGFVGLIVPHMLRLAGGSD
                     HRLVLPGAVLGGGTLLTVADLIARSVVAPRALPVGVITAALGVPLFLYLLLRTRRVL"
     gene            4967..5590
                     /locus_tag="VFX38_02640"
     CDS             4967..5590
                     /locus_tag="VFX38_02640"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEX5313789.1"
                     /translation="MERVTESIDREGFRANVGIILSRDGGEVLWARCVKNRGWQFPQG
                     GIDVGETAEEALFRELYEELGLGAGDVRITGYTEGWLRYRLPETYQRKHASPRCVGQK
                     QVWYVLRLISPETTIRLTAVDHPEFDRWRWVDWWHPLSDVIFFKRRVYDQALTQLAPF
                     MFPEGPPPRPGRQAPAGWYAPRSPYHIRRGAQRRESRSQSETARELR"
     gene            complement(5526..5939)
                     /locus_tag="VFX38_02645"
     CDS             complement(5526..5939)
                     /locus_tag="VFX38_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020159979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX5313790.1"
                     /translation="MRWVLAFHIIFMVTWFAGLFYLPRLFVYHEAAREAALRERFAVM
                     ERRLFVIMTIGMAATVGFGIWLVAGWFWPLPLWLEVKLALVAALLVYHFQCLRMMLRL
                     ARGARVPSQRALRYFNEVPALFLIAIVILAVVRPA"
     gene            6076..6807
                     /locus_tag="VFX38_02650"
     CDS             6076..6807
                     /locus_tag="VFX38_02650"
                     /inference="COORDINATES: protein motif:HMM:NF042896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HEX5313791.1"
                     /translation="MAPRHRVVVKREHHAHRPIVIGLVVVAVVAGLVALAATFYFGER
                     WGGYRQFASHAEKAKISRALAARSSQAAALTDRVAFLERSLSLANASASALKANVVKQ
                     QAKQVDLEQKVAFYQRFMQPAAGDGAKVRIAGLQLLPTGRAHDYRFQIVLVRADGKTS
                     PTLEGRCKLEVSGTRKGKPESLSLAALSPRGAEPLDFTLRYFTNLSGALRLPAGFSPE
                     AVKVEVEMKGEPEIAGTYSWPMFRG"
     gene            6835..7239
                     /locus_tag="VFX38_02655"
     CDS             6835..7239
                     /locus_tag="VFX38_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014705372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX5313792.1"
                     /translation="MRKDKAGSGTAIDTLIGSGTTITGDINFTGGLHLEGAIVGNIAG
                     NDGSARLDVSEQGRVAGEIHVATAIVSGSVEGDLHAGERLVLGQRARIDGNVFYHVLE
                     MAAGAQVNGKLVYRPGNAEPLALEHRAANDRA"
     gene            7271..7624
                     /gene="erpA"
                     /locus_tag="VFX38_02660"
     CDS             7271..7624
                     /gene="erpA"
                     /locus_tag="VFX38_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEX5313793.1"
                     /translation="MNDATVADTEELILTAAAAARIRALIAEENNPALKLRVYISGGG
                     CSGFQYGFGFEEEARADDQALERHGVTVLVDPLSLPYLEGAEIDFADDLQGERFIIRN
                     PNASTTCGCGSSFSA"
     gene            7640..8131
                     /locus_tag="VFX38_02665"
     CDS             7640..8131
                     /locus_tag="VFX38_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX5313794.1"
                     /translation="MSAGLRLLVMRHGPAEAAGPAGDAARALSVEGRERMRLGLAALA
                     RILPAPRRIYTSPLVRARQTAELLAAAFDADSPVVTPLLAPGFDRARLIGELARAALE
                     PLAIVGHEPDLSGFVASALGASGSVEFDKGSACLLEMLDAHRAVLRALYPLEAMTRLA
                     RPG"
     gene            complement(8171..>9242)
                     /locus_tag="VFX38_02670"
     CDS             complement(8171..>9242)
                     /locus_tag="VFX38_02670"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEX5313795.1"
                     /translation="GTSMDGVDAALVRFAGARVEPIAARTTPYEMALRDALDLLAAGR
                     ATRDPIDELGRLDAAVGEAFGAAASSLLVEAGVAPQDLCAIGSHGQTIRHRPDLGFSI
                     QIGDPARIAQASGITTVADFRRADLAAGGEGAPLAPIFHRAFFADPDETRGVLNLGGI
                     ANLTLLAPGRPVIAFDVGPANILLDPLMREMTGEDCDRDGRLAATGRVDETLLAALLA
                     DPWFARPPPKSSGPEYFHLAWLRRHSRGAALAPADLAATLTELSAAAVALALDAQGIR
                     PARIFGCGGGVHNPELMRRLAARLPATAITTTAELGVDPDYVEAICFAWLAGETLAGR
                     PGNLPEVTGARRAVVLGSVFHP"
BASE COUNT         1716 a         3118 c         3070 g         1338 t
ORIGIN      
        1 cttttgttca gcgcgtccgg attggcattc gaatccacaa tctgcgccca gccggcggca
       61 ttgctcgcac catccggaaa cgcgccacgg agcagcgaga tgacgctcgc cacgttgggc
      121 gcggcggcgg aggtgccgta aaacggcgac ggaaacccgc ccgcgccgct gaccgtgacg
      181 ccgtcgatgc cgacgagatc gggtttgggc ctggtctgaa ttccggactg ccagtcccca
      241 taggtgtacg ggccggagtc gctgaagggc tccatgtccg cgattgcggg aggtcctccc
      301 accacctgcg cagcgccgac ggcaagttcg cccgcgaggc ccgcacggcc gaagatcccg
      361 cccttggtag cgtactgaaa tgttccggcc ccgtcgagaa acccgttgag cttgatcaga
      421 atggggtgcg cgcactggaa acaaagcgcc gcgagataga gcgtctgatt cgagccggtc
      481 ttgttctgat agtaaatgta ctccccgtcg ccgatgcccg gatcgggccc ctgcgtctga
      541 tcataggtgc tggacgcgac gacggtgccg ttggccttta cgagaaagag atccacgtca
      601 tcgtagggtc cgcccgcggg atcgttccag gtgagaaagg cgttgagcgc gatggcggga
      661 tcgagcgagg tcggcgcgat gagggtgggc tcggtcgcat cgctgtgttg atggacggca
      721 gcgccgaaat tcatcgccga ctgatagctc cgcccaccgg cctggccagg tccgggcgtg
      781 tagtccggat gcaggctgat ccggttggga tgggtgtcgg caaggtaggc atgctgaaaa
      841 taatccagcg catcgttgcc cgctgccgtc gccagattga tttgcgggtg agcggcgatg
      901 aagtcgccga ccgcattcac gagcgcatcg ccgtcaggct gattgaagag gaaggcggcg
      961 aagaatccga gatcgtccac aatcacatcg gcattgaact cattgtcgag atcgtcgaga
     1021 caggtaacga agtccacggt agtcgccggt ccgcagaatc cgagtgcggc gccgggcgcc
     1081 atcgcatgga cctcctccag cattgcggta ccttccgcgc cctcgcccgc caggcccggc
     1141 gccacccata cgttgttcgg caggtatccc gcattctgcg actccgatag tccgtcgacg
     1201 ccaacggaaa tgacaccgac gcgcagcccg gaaccgtcca tgcccgcatt caccaagggc
     1261 tgcacgtcaa gtcctgagat gccttcacga tcaatgcgaa ggcccggagg aacgccgccg
     1321 gctcgcggca ttgcatccgg caaggtcgcg actttcgcgc ggccgtaggc gggcaagtcc
     1381 acgcgtacca ccgcggcgag ttgagacgtt tcgcgcaacc gcccgatcgg cacccaagcc
     1441 tgcacgatgc caagcgcggg gctgaacacg acgttctcgg cgcccaaggt cgcgagtgcc
     1501 gccgctgccg gcatttcgcc cggtcggaaa cgaatgtaaa cctcgacccg tcccgtcgcc
     1561 gatacgcgag gcgcgaagat ttgcgtttgt acgccggacg ccgcggtgag acgcgcgagc
     1621 gtcgccaccg gcgcctgccg ccaggaatcg gcgagcgtct gcagccgggg cgacaggctt
     1681 ccggccgcag ccggaagcgc aaaggcaaac gtgatcgcgg ccagcacgat gccccgaatc
     1741 cgatgtcgct ttctcatgac cttgcccctc caaaacgatc tgccagtgaa ggaaaaccga
     1801 gacccggttc acggcccgcc gaaggcggcc ccgctcctgg gaaaagcgta cgccggagcg
     1861 gagattcgaa gcacgccggg caatttcgcg acgcgctcga tggccaccgg cgacgcccat
     1921 gcctgatatg ttcgcagcgg cgcggatgca cggatatccc gcgctcccgc gcccgccagc
     1981 gccgccttca tgggaggcgt tacctcacgc acatgcacgt aaatctcgat ctcgcgccgc
     2041 cggctgacat gaacgcggga atcggagagc gccgctgctt cggtgccatt cgccgtcgcc
     2101 cgaacccggt ttgcaaccgc gagcagcatc ggtgcaatct tgttttccgc cgcggcgtcc
     2161 gcgctcgccg cgttgcgggc gctggtgttc gaagcaaccg cggcggtagg cgagacgacc
     2221 gttgcgtccg acgacgcatg gcccgcgccc gaacggggcg gcgcaccgca gccggcgaga
     2281 agggccgcgc ccgtcacgac ggaaagcatc cgcagcgaaa aaaccattcg caaccccggc
     2341 atgtccccgg aagaccacct tagaccaaat gggctcactc tgcccggccc gagcgctgcg
     2401 ccggcgcaac gacgagcgac ttcgcacacc gatcctcgcc gcgcatcgcc gcttcccgag
     2461 tcctctgcgc gtgaatacgc gccgacgtac taaaataccg cgccgcaacg caggaatcgg
     2521 aaagtggact ttaccaagct cggccccggc aagaatccgc cggacgatct caacgtcatc
     2581 atcgagatcc cggcgcaggc ggcgccggtc aaatacgaac tcgacaaggg cacgggcctg
     2641 ctcgcggtcg atcgttttct cagcacctcc atggtgtatc cggccaacta cgggctgatc
     2701 ccggcgaccc tctgcgacga cggcgatccg ctggacgtgc tggtcgtcac gccgctgccg
     2761 ctcatccacg gggcgctgat ccgcgtgcgc ccggtggatc tgctcgagat gagcgacgag
     2821 aagggcggcg acgccaagct ggtggcggtg ccggtgacgg gggtgtatgc gggctatggc
     2881 gaggcacaat cggtagacga ccttcccggc ggcctcaagc accagatcac gcatttcttc
     2941 gaaaactaca aggccttcga acccggcaaa tgggtcaaac tcgagggttg gggcggtctc
     3001 gaacgcgccc gcaaggaagt gctcgactgt atcgaacgct gtcgcggacc ggcagcgggc
     3061 tgaaaaaccc gattcgacac gacccgacct tgtttccggc ggcgggtgcg ccgccgccaa
     3121 cgccaacgcg ccgtcagccg ccaacgacgt agaacggcag gccttccatc tcgagtacgc
     3181 gcactcggca gccgtaggct gcggcgatcg gtccggcccg caggacttcg cgtgccgacc
     3241 cgaccgcggc gccgccacgc ccgtcgagca ggaccgcccg atccgcatgg cgcgcggcaa
     3301 gcgacagatc gtgcaggctc agcatgattc cggccccggc gagtcgcgcg agcccgcgaa
     3361 gcaggcgcag gagacgtgcc tgatacgcga agtcgagcgt ctccagcggc tcgtcgagca
     3421 gatagatttg cggtgcctgc gtgagcagag tcgcaatcgc gagacggcgg cgctctcctc
     3481 ccgaaagtgt cgcgatcgcg cgcgcggccg ccggggcgag tccgacccgg cgcagcgccg
     3541 cacgcgcctt ggcgacatcc gccgccgatt cccattgcca gaagccgata tgcggatgcc
     3601 ggccgatgag cgccgtctcg agcacggtcg ccggataagg atcctggctg tcctggggaa
     3661 gaaagccgag acgggaggcg atgcggcggg gggacaggcg cgcgagcggt ctcccgtcca
     3721 tgaacacccc gccgctttcg ggaggaagca gacccgcaag ggtcgcaaga aggcttgatt
     3781 tgcccgcgcc gttcggtccg accaccgcca cgatttcgcc cgcggcgaga gaaaaatcga
     3841 aatcctccac caggacacgt cggccgcgcc gcactccgag cccgcggcag gcgagaagtc
     3901 cgctcacagc accctgcgcg tgcgaagcag gagatataga aagagaggaa ctccgagcgc
     3961 cgccgtaatg acgccgaccg gaagcgcccg gggcgcgacg accgaacgcg cgatcaggtc
     4021 ggcgaccgtg agaagggtcc cgccgccgag gaccgcgccc ggaaggacca ggcgatgatc
     4081 ggaaccgccg gcgaggcgca gcatatgcgg cacgatcagc ccgacgaaac cgatggtccc
     4141 cgcctcgacg accgcaaccg cggtcagtac ggatgcggcg atgtagagcc cgatggtgag
     4201 cggtcgaacc gacactccga gggcgcgggc ctgcaactcc ccgcgggcaa gcacgttgag
     4261 cggtcgggcg cagggaagac tgagcaccag cgccgccgca agcacggcga gcggcaactg
     4321 ccagtcgctg cgaaaggaaa gatcgcccat gagccagaaa agcatgctgc gcagcgtcgc
     4381 atccgggctc agcgagagaa ggagcgcgat ggccgctccc cagccggcgg cgatgacgac
     4441 tccggtgaga agcaggcggg tcggtgccca gcttccccgc ccgtgcgcga gcacgaatac
     4501 gacgagggtg gtcgcgagcg cgcccgcaaa ggcgagtccc gtcagagccg ccgtgccgag
     4561 acccacgagg atcgcgatca gcgcgcccgc cgcggcgccg cccgaaacgc ccagaatata
     4621 gggatcggcg agaggattgc gcagcagcac ctgcatcagt gcgccggcaa gcccgaggag
     4681 cccgccggtc gcgaaggccg cgaggctgcg cggaagccgc agcgacagca ccagcgcatc
     4741 ggccagacgc gaacctcgcc cgccgatcgc cgccagcacc tcgccgggac cgagcgcgac
     4801 gcttcccaca aaaagcgagc cgacgagtgc ggcggcggcc gcaacggcga gcagggccag
     4861 aatcaggcca ctccgacgca acgcgggaag ccgcaactca gccatcggaa acagttcgcg
     4921 atttgggggt tatactgcga caaagcgcga cggaactgcg gacgcaatgg aacgcgtcac
     4981 cgaatccata gaccgggaag gttttcgcgc caacgtcggg atcattctca gccgcgacgg
     5041 cggggaagtg ctctgggccc gctgcgtcaa gaaccgcggc tggcagtttc cgcagggcgg
     5101 gatcgacgtg ggcgagaccg ccgaagaagc actcttccgg gaactctacg aggaactcgg
     5161 cctcggcgcc ggagatgtcc gcatcaccgg ctacaccgaa ggctggttgc gctaccgcct
     5221 gccggaaacc tatcaacgca agcatgcaag cccgcgctgc gtcgggcaga agcaggtctg
     5281 gtacgtgctg cgcctgattt ctcccgagac cacgattcgc ctgacggccg tcgatcatcc
     5341 ggaattcgat cgctggcgct gggtggactg gtggcatccg ctgagcgatg tgatcttttt
     5401 caagcgccgc gtctacgatc aggccctcac ccagctcgcg ccgttcatgt ttcccgaggg
     5461 gccgccgccg cgacccggac gccaggcgcc ggccggctgg tatgcaccgc gcagtccgta
     5521 tcacatcagg cggggcgcac aacggcgaga atcacgatcg caatcagaaa cagcgcggga
     5581 acttcgttga aataacgcag cgcacgctgt gagggaacgc gtgcgccgcg cgcgagccgt
     5641 agcatcatcc gcagacactg gaaatgatag acgaggagcg ctgcgaccag cgcgagcttc
     5701 acctcgagcc acagcggcag cggccagaac cagcccgcga ccagccagat tccgaagccc
     5761 acggtcgccg ccatcccgat cgtcatgatg acgaaaagcc gtcgctccat tacggcgaag
     5821 cgctcgcgca gtgctgcctc gcgcgcagct tcgtgataca cgaagagccg cggcaggtaa
     5881 aagagcccgg cgaaccaggt caccatgaaa atgatgtgaa aggcgagaac ccagcgcatt
     5941 tttcgcccgt ttttgcgatt gccgattata ggcgcgcacc gacgccggct tcgggaaagg
     6001 cgcggctgcg ggaaccattc gggccgcagg ctttcgatta tgatgccctc cgagcggatc
     6061 ggaccgaacg gtcgcatggc gcccagacat cgggtcgtag tcaaacgcga acaccacgcc
     6121 caccggccga tcgtgatcgg cctggtggtg gtcgccgtcg ttgccgggct cgttgcgctc
     6181 gccgccacct tttatttcgg cgagcgctgg ggcggatatc gccagttcgc gagccacgca
     6241 gaaaaggcaa agatcagccg cgcactcgcg gcgcgctcct cccaggccgc ggccttgacg
     6301 gatcgggtcg cctttctgga acgctcgctt tcgctcgcca acgcaagcgc ctcggcgctc
     6361 aaggccaacg tcgtcaagca gcaggcgaaa caggtagatc tggagcagaa agtcgccttc
     6421 tatcagcgct tcatgcagcc ggccgcaggc gacggcgcca aggttcgcat tgccgggctg
     6481 cagcttctgc cgaccggacg ggcgcacgac taccgttttc agatcgtgct cgtgcgcgcc
     6541 gacggcaaga cgagtccgac gctcgagggc cgctgcaagc tcgaagtgag cggcacccgc
     6601 aaaggcaaac ccgagagcct gtcccttgcc gccctcagtc cgcgcggagc cgagccgctc
     6661 gattttacgt tacgctattt caccaatctt tccggcgccc tgcggctccc cgcgggcttc
     6721 tcccccgagg ccgtcaaggt cgaagtcgag atgaagggcg agcccgaaat cgccggaacc
     6781 tacagctggc ccatgttccg cggctgaacg gggctcgaac ggaggattcg cgcaatgcgc
     6841 aaagacaagg ccggttccgg cacggccatc gacacgctca tcggatcggg gaccacgatc
     6901 accggtgaca tcaatttcac gggcggtctg catctggaag gagccatcgt cggcaacatc
     6961 gccggcaacg acggctccgc ccgactcgac gtgagcgagc agggccgcgt cgccggcgag
     7021 attcacgtcg cgaccgcaat cgtcagcggc tccgtcgagg gcgatctgca tgcgggcgag
     7081 cgcctcgtgc tcggacagcg ggcgcgcatc gacggcaacg ttttttatca tgtcctcgaa
     7141 atggcggcgg gagcccaggt caacggcaaa ctcgtctacc ggccggggaa cgccgaacca
     7201 ctcgcgctcg aacatcgcgc cgccaacgac cgggcttgaa acaggaccgc gggaagtcca
     7261 ggatgaaacc atgaatgatg ctaccgttgc agacaccgaa gaactgattc tcaccgcggc
     7321 ggcggcggcg cggattcgcg ctctcatcgc cgaggagaac aatccggcgc tcaaattgcg
     7381 ggtctatatc tcgggcggcg gctgctccgg ctttcagtac ggcttcggct tcgaggagga
     7441 ggcccgagcg gacgatcagg cgctcgagcg gcacggcgtg accgtcctcg tcgatccgct
     7501 cagcctgccg tatctcgaag gcgcggagat cgacttcgcc gacgatctcc agggcgagcg
     7561 cttcatcatc cgcaatccga atgcgtccac gacctgcggc tgcggctcct ccttctccgc
     7621 ctgagttttt cgccgcccga tgagcgccgg cctgcggctg ctggtgatgc gccacggccc
     7681 ggccgaagcc gccggccccg cgggcgatgc cgcgcgcgcg ttgagcgtcg aggggcggga
     7741 gcgcatgcgg ctcggcctcg ccgccctcgc gcggattctg ccggcgccgc gaagaatcta
     7801 cacgagcccg ctcgtgcgcg cccggcagac cgccgagttg ctcgccgccg ccttcgatgc
     7861 ggattcgccc gtcgtgacgc cgttgctcgc gccgggattc gaccgcgcga gactcatcgg
     7921 cgaactcgcc cgcgccgcgc tcgaaccgct cgccatcgtc ggacatgaac cggacctttc
     7981 cggtttcgtc gcctcggcac tgggcgcgag cggcagcgtg gagttcgaca agggcagcgc
     8041 ctgcctgctc gaaatgctcg atgcccatcg cgcggtgctg cgcgcactct atccgctgga
     8101 ggcgatgact cgactcgccc gtccgggctg agaatcgggc ttcctgcgtt cggcccagac
     8161 tgccccgctc tcaggggtga aaaacggagc cgagcacgac ggcgcggcgg gcgcccgtca
     8221 cttccggaag attgccgggg cggccggcga gcgtctctcc cgccagccag gcgaagcaga
     8281 tcgcctcgac ataatcggga tcgacaccca gctccgcggt cgtggtgatc gccgttgccg
     8341 gcaggcgcgc ggcgaggcgg cgcatcagtt ccggattgtg cacgccgccg ccgcagccga
     8401 agatgcgcgc ggggcgaatg ccctgcgcat cgagggcgag cgccactgcg gccgcggaaa
     8461 gttcggtcag ggtcgcggcg aggtcggcgg gtgcgagagc ggcgccgcgc gaatgccggc
     8521 gcagccaggc gagatggaaa tattcggggc cactgctttt cggcgggggg cgcgcgaacc
     8581 agggatcggc gaggagcgcc gcgaggagcg tctcgtccac ccgcccggtg gcggcgagac
     8641 gcccgtcgcg atcgcagtcc tcgcccgtca tctcgcgcat cagcggatcg agcaggatgt
     8701 tcgccggccc gacatcgaag gcgattaccg ggcgtcccgg cgcgagcagc gtgagattgg
     8761 cgatgccgcc gagattgagc acaccgcgcg tctcgtcggg atcggcaaag aacgcacgat
     8821 gaaatatcgg cgcgagcggc gcgccttcgc ctcccgccgc aaggtcggca cggcgaaaat
     8881 ccgcgaccgt cgtgattccg ctcgcctggg cgatgcgcgc cggatccccg atctggatgg
     8941 agaagccgag atcgggacga tggcgaatgg tctgaccgtg gctgccgatc gcgcacaaat
     9001 cctgcggcgc aaccccggct tcgacgagta atgacgatgc ggccgctccg aaggcttcgc
     9061 ccacggccgc atcgaggcgc ccgagttcgt cgatcggatc gcgggtcgcg cgtccggccg
     9121 cgagcagatc gagcgcatcg cggagcgcca tttcataggg agtggtacgc gcggcgatgg
     9181 gttcgacacg cgcacccgcg aatcggacca gtgcggcatc cacgccgtcc atgctcgttc
     9241 cc
//
LOCUS       DASUJG010000131        14148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_403897, whole genome shotgun sequence.
ACCESSION   DASUJG010000131 DASUJG010000000
VERSION     DASUJG010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 14148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14148
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403897"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            303..2594
                     /locus_tag="VFX38_02675"
     CDS             303..2594
                     /locus_tag="VFX38_02675"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313796.1"
                     /translation="MPPALRPALYQALAKDAGPAYAINKKGCATLPKTALKACFGPGG
                     ARFAGARELSLHLAAFGRGARLAPVAAVNPVIEANRVSYRHGNLSEWWRVLPMGFEQG
                     FTIAHRPAGDGDLVLALTSRRPGAFITRHSRADGNPVRTSPSIPLRSEGGKELAWGKL
                     RYGHLVVTDAKGKVVPATLKTSGDRILIAVNDARAAYPLTVDPLIWLEQQVTGSGDNN
                     SFGYSLAVDGTTALFGAPDANDPNGNEDVGVVYVFNYSEGVWSETQELTASDAQYQAE
                     FGWSVALDGATAVIAAPAHEVGGIYVLEGAVYVFNYSGGVWSETQEFIASDGQPFGLF
                     GTSVALDGTTALISAGQLVAAEAYIFSESGGIWTQTQTLVPSDDGVYFGDSVALDGTT
                     ALIGAGSVPTPSGRAVYVFAHSGGTWTQTQELTPDDAADVGFGVSVALDGSTAIVGNP
                     SPSDGSIGTADIFSESGGTWTQTQVLSASEEPFGASVALDGTTALISASYYGTDYLFS
                     AAGGTWTQTQKITDAGGAVALQGGTAVVGGDDDAFFLDQSDLDLAPNAPESVAPGANY
                     VFQTIATNNASAASPAVALTVPIPAAASFVSATATEGQCYNNPASVYCYFGSIAGNAG
                     TATANITFKATGSSGTTIVNVSHVLDASPSLSAEALTDIVEGGTCPDGYTKYERTLAA
                     GEKSYSPPYDAPAGEENAILTAPDGFQLYGVYGVFYSGSWHGTKYRIPGNEVHRWAPA
                     GRFMWIVTAGDEGGAYTLCILHP"
     gene            3016..5745
                     /locus_tag="VFX38_02680"
     CDS             3016..5745
                     /locus_tag="VFX38_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5313797.1"
                     /translation="MARPADFIWRFGEVELDGRSLGLTVGGQPTAIEPKPLALLLYFL
                     THPGEAVSRDELLEALWPGRIVVEAALTNCVHKLRSLIGDERATTIQTVRGYGYRLLA
                     PDLQVEKLPEHASSPITAPTMKAGETPPGRAMWRLLRCLGSSDYGEVWLARHVKLPEQ
                     RVFKFARANATSLTALRREITLHRLMRNSFGHCEWLVNVLDWNLTDAPFFIELEYVPG
                     GSIGDWAASGGGIGEIPLAMRIDLVVQAARALSAAHSLGVLHKDLKPANLLVDTSDPE
                     RPRLRVSDFGSGRLLDDERLGALNITRLGFTHTGLRSDTDSGTLLYMAPEVLSGQPAT
                     IQADIYALGVILYQMVAGDLGKPLAPGWEHDIEDELLREDIARAVAGDPERRLASAAE
                     LADRLEELDARRTEREREHRRAEEAEKTRVALVRARARRGLLQALVATFFVAAIVTGL
                     LLWQTERERSAAQREARLAQSVTHFLTDDLLSSANPMIAGSRDVTVRQILDAASAKLD
                     QRFAHQPLVKASLERVIGTAYAAMDDRKQAEPLLLAAERGLAAQLGNAAPETEETRLA
                     LRDLYLDLFDIKGTVAVSKRIDSAERAAGVPNPEAEYEARAMLIAMPAIARAGAYWLT
                     DLAAPMNQLYVEAGKQLGLNNRATARMLWYTGVTLSWEHRYREAAPIDREVLRQFQVI
                     YGPDHPRTLEAAIYLAQALDHTGRAAQARPLLVKMIADFEHTLGPDHVFVLNSRRFLA
                     ESDLLTGKPEAAVKEMRGVYARRKTLLGETSRLAEADALHLGKILAAAGRPAEALPIL
                     ERLLHLQEQDLGVASPQAMLTRDALGKVLVQSHQDRDAEALLEKNYRLAAARLHGGWF
                     LGETAFQLGKVLERNGKPDRAKPLLEEAATVFTTALGPNNPMTEMAEQELASS"
     gene            complement(5889..8039)
                     /locus_tag="VFX38_02685"
     CDS             complement(5889..8039)
                     /locus_tag="VFX38_02685"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEX5313798.1"
                     /translation="MRFFKHVAIAAVAAAPLIALAAGEADVPKAEVEALHLSWLDKAV
                     SPSTNFFEYANGGWQKQNPIPAAYSRWGTFNLLQIRNEKIVREILEDAAKADAKPGST
                     QQKVGDFYASGMDMARIDKEGAKPLAPEFARIAAINNTDDLQQEVARLQMMGVNAMFG
                     FGSMQDFKDSSQVIGAAFQGGLGLPDRDYYLKTKDQCAADRKAAAEGTSPAAHGASAA
                     KAAEAAYTDCTAQAAKFQQVRDAYVAHIAAMFELLGDSKTQAAAEAQTVMKIETGLAQ
                     ASMSQVAQRDPHAIYHVMTLAKLDEATPNFSWQEYFSAIGVPDLQSANLAMPDFFKAL
                     GDDLKSVPLADWKTYLRWHLVDSFAPYLSKPFVDEDFKMQSALTGAKELRPRWQRVVA
                     SEDDALGFAVGKLYVEKEFPPSSKKAVLAILHNIRGALKDDLATLSWMTPATREAAIK
                     KLGMMGERIGYPDKWRDYSKLTITKGPYVDNVIAANVFLTKRDLAKIGKPVDRSEWFM
                     TPQTVNAYYDPSMNNINFPAGILQPPFFDPNAPAAVNYGALGFVMGHEMTHGFDDQGA
                     QFDGHGNLKNWWTKEDLKKFKAATSCISDHFSQYTVDNGLHVQGKLVTGEATADFGGL
                     TLAWRAFHASPAYKEAKTIDGFTPDQQFFLGAAHVWAMNIRPEESRRLVTIDPHPPAM
                     YRVNGTLANMPQFQQAFDVPADSPMVNKDRCKIW"
     gene            complement(8180..9319)
                     /gene="cfa"
                     /locus_tag="VFX38_02690"
     CDS             complement(8180..9319)
                     /gene="cfa"
                     /locus_tag="VFX38_02690"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HEX5313799.1"
                     /translation="MTTTAPSGTAGRLERKAARLLALADVRIGGDRPWDIQVHDPRLF
                     ARVFAHGSLGFGESYMDGWWDAERLDELLTRVIGARLDRRMALPQKAARWLLGQLVNL
                     QSGRRAFTVGKEHYDRGNDLFERMLDRRMTYSCGYWKDATTLDEAQEAKLDLACRKLG
                     LEAGMRLLDIGCGWGGMAKFAAERYGVSVVGITVSKEQAEYGQHLCAGLPVEIRVEDY
                     RAHRGAYDRIVSIGMFEHVGHRNYRRYFTTARRLLKPDGLFLLHAIHGNVSLTRTDPW
                     IEKYIFPNGMIPSPRQVTRAIERLFVIEDLHNFGADYDKTLMSWYANFERAWPELRER
                     YGERFRRMWRFYLMASAASFRTRSDQLFQILLSPGGVPGGLRVPR"
     gene            9765..11990
                     /locus_tag="VFX38_02695"
     CDS             9765..11990
                     /locus_tag="VFX38_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX5313800.1"
                     /translation="MASENTRSHVFGAFRLQGADRRLFHDGVEVPIAPKAFDLLQVLV
                     ENPGRLLSREELIQALWPRTIVTEHNLTSTLYQLRKALGDEGSPPRYIETARGHGYRF
                     IASVSLIDAGGRPVPDGSRPTARYEHPARRLRRAALAAAVVVVVVGAAIFLWRWNSAA
                     KSGDDRTATPVIAVLPFTNLSADKANAYLTQGIQDTILTRLAAISGLRVISHTSTESY
                     ASRPENLKTVARQLGASEILEGSVQKAGDRVQVNVQLIDARTDTHLWAKTYDRDLENL
                     FGVEGEVAANVTQALQTKLLPTETASLSRPPTRDAQAYLLYLKANHYTDEAFYRTNAK
                     DPASTVAKATALYQQAIARDPRFALAYARLSLLDAFAYWFEIDPSQIRAAAAELAAKR
                     ALALDPALPPARFAMGYVEYYCHRNYPAALRQFNLASLGLPHNAAVTGAIAFIHRRQG
                     KWDEALDGLAKAAAFDPRNPSWFTEMGNILMALRRYPEAVEKFDQALAIAPDSYDAAG
                     DRVAAWFMAGKLAQASKALAQIPPGVDPQGLASMLRFQIAWLARAPDRALAALATGPT
                     QMAGPETLITVPKSLLEARVWTLKGDPGKARPLYQDARDKLLAELHRRPESPDLWSAE
                     GLAAAGLGEKAAAIRAGRRAVKLFPPSKDAMAGPVYLTNLAEIYAISGEAKPVVPLLR
                     RLLAMPAGASVSIPVLRMNPIWDPVRKDPGFQALLRRRGGAAPAVPASVAVAIGTGVK
                     R"
     gene            complement(12018..13847)
                     /gene="aspS"
                     /locus_tag="VFX38_02700"
     CDS             complement(12018..13847)
                     /gene="aspS"
                     /locus_tag="VFX38_02700"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX5313801.1"
                     /translation="MRSHFCADLNASLIDRTVTLAGWVHRRRDHGGVIFVDLRERSGL
                     VQIVFDPEADEAFARAGRLRGEWVIEVTGRVRPRPAGTENPALASGEVEVLAAEVKIL
                     AAAATPPFTPDEEEVGEDLRLRYRYLDLRRPLMQERLRARHRIIRRLRAFLDDEGFIE
                     IETPCLTKATPEGARDYLVPSRTHRGKFFALPQSPQLFKQLLMLGGLERYYQIARCFR
                     DEDLRADRQPEFTQLDIETSFLDEHAITALMERMLRELFAGELGVDLGEFPRLSYADA
                     VARFGTDRPDLRNPLELVEIADLVVDSEFKVFSAPARDPAGRVAALRLPGGGDLSRGA
                     IDGYTELVSRCGAKGLAWIKVADRAAGREGLQSPIVKFLAEETLTAILDRSGAANGDI
                     LFFGAGAAKIVNESLAALRVRLAEERGLQRAGWAPLWVVDFPMFEWNEEEKRWQALHH
                     PFTAPSESDPAALAGAPGQLLSRAYDVVLNGYEIGGGSLRIHTPEMQRAVFAVLGIAD
                     IEAEEKFGFLTEALRYGAPPHGGIAFGLDRLVMLMTGAPNIREVIAFPKTQTAQDPMT
                     GAPAEADARQLAELGIRPLKLPAVTSGAGGAAASGDSPPRRIS"
BASE COUNT         2505 a         4654 c         4530 g         2459 t
ORIGIN      
        1 aacgaagggg gccggcgcct cagcgccggg gggatgtcct gcccgagccg caagtccgcg
       61 gccgttagca aatggttaga aaacggtttt ctgttcgtga ggtaaaagcg gtttgttcct
      121 gccatagtct ccgggcattc atccactcgg ggacttgtcc catgaaacct tcgtcctttt
      181 ccgtgcttcg tgccgcccgg agtgccgggc ttggctcgct actcatgagc ctttctcttc
      241 tcgccattgg ttttggggcc gggtcggctc cggctgccac cgccacgaca acgagcacgg
      301 cggtgccgcc ggcgctgcgt ccggcgctct accaagcgct cgcgaaggat gccggtcccg
      361 cctacgcgat caataagaag ggctgcgcca ccttgccgaa aacggcactg aaggcctgct
      421 tcggtcccgg cggcgcccgc ttcgccgggg ctcgggagct gtcgttgcat ctcgccgcct
      481 tcgggcgggg cgcgcggctc gcgccggtgg cggcggtgaa cccggtcatc gaggccaatc
      541 gggtaagtta ccgccacggc aacctcagcg aatggtggcg ggtgctgccg atgggcttcg
      601 agcagggctt caccatcgcc caccgtcccg ccggcgacgg cgatctcgtg ctcgcgctca
      661 cctcccgtcg tcccggcgct tttattactc gtcattcccg cgcagacggg aatccagtca
      721 ggacatcccc ctcgatcccc cttcgttccg aagggggaaa agaactggcg tggggcaagc
      781 tgcgttacgg ccacctcgtc gtcaccgacg ccaaaggcaa ggtcgttccg gcaaccttga
      841 aaaccagcgg cgatcgcatc ctgatcgccg tcaacgacgc ccgcgccgcc tatccgctca
      901 ccgtggatcc gctgatatgg ctcgagcaac aggttaccgg cagcggcgac aataattcgt
      961 tcggctactc gctcgctgtc gacggcacga ccgccctttt cggtgcgccg gatgccaacg
     1021 accccaatgg taatgaggac gtgggcgtgg tttacgtgtt caactattcg gaaggcgtct
     1081 ggagcgagac gcaggaactc actgcgagcg atgcccaata tcaggccgag ttcggctggt
     1141 cggtcgccct cgacggcgcc accgccgtca tcgccgcgcc tgcgcacgaa gtcgggggga
     1201 tttatgtgct cgaaggcgcg gtttacgtgt tcaactattc ggggggcgtc tggagcgaga
     1261 cgcaggaatt catcgcgagt gacgggcagc ctttcggtct tttcggcacc tcggtcgccc
     1321 tcgacggcac caccgcgctc atcagtgccg gacaactcgt agccgctgaa gcctatattt
     1381 tcagcgaatc aggcgggatc tggacgcaaa cgcagacgct cgtgccaagc gacgacgggg
     1441 tatacttcgg cgactcggtc gctctcgacg gcaccacggc tctgatcggc gctggctcgg
     1501 tcccaacgcc gtccggcagg gcggtctacg tgttcgccca ttcgggcggc acgtggacgc
     1561 agacccagga actcacaccg gacgacgcgg ccgatgtcgg cttcggcgtt tcggttgccc
     1621 tcgatggcag caccgcgatc gtcggtaatc ccagtccctc tgacggttcg atcggtaccg
     1681 ccgatatatt cagcgagtca ggcggtacct ggacgcaaac gcaggtgctc tccgcgagcg
     1741 aggaaccctt cggcgcgtcg gtcgccctcg acggcaccac cgcacttatc agcgcgtcct
     1801 actatggcac ggattatcta ttcagcgcgg cgggcggcac gtggacccaa acgcaaaaga
     1861 ttaccgacgc gggcggtgcg gtcgctctcc agggtgggac cgcggtggtc ggaggcgatg
     1921 atgacgcgtt tttcttagat cagagcgatc tcgatctcgc gccgaacgcg ccggagtctg
     1981 tggcgcctgg cgccaactac gtcttccaga cgatcgcgac caataatgcg agtgccgcct
     2041 cgcccgcggt ggcactcacc gtgccaatcc ccgccgcggc gagcttcgtc tccgccaccg
     2101 cgaccgaggg gcagtgctac aacaatccgg cttcggtgta ttgctacttc ggctcgatcg
     2161 cgggtaacgc gggcacggcc acggccaaca ttaccttcaa ggcgacgggc tcgtccggca
     2221 ccacgatcgt taacgtttcc cacgtcctgg atgcatcgcc gagtttatcg gcggaagcgc
     2281 tgacggacat cgtggaaggc ggcacctgcc ctgacggcta cacgaagtat gagcgcacgc
     2341 tggcggcggg cgagaaatcg tactcgccac cctatgatgc gccggcgggc gaggagaacg
     2401 cgattctcac ggcaccggat gggttccagc tctacggagt ctacggagtc ttctattctg
     2461 ggagttggca cggcacgaag tatcgcattc cgggcaacga agtgcaccgc tgggcgccgg
     2521 ccgggagatt catgtggatc gtgacggccg gcgacgaagg cggagcctat accttgtgca
     2581 tcctgcatcc gtgatgcgtt aacagactgc cgcttgatca agcccccgcg gcattctgtg
     2641 ccgcgggggc ttttctttcg cccccttcgg aacgaagggg gccgcgctca tgcgcggggg
     2701 gatgtgcggc ggcgaagaag acatccccct cgatccccct tccttcggaa gggggaagca
     2761 gcagtattta cccccttccc gcggaagagg gaagtgtatc ttcgtttctg gctcctcgtt
     2821 ccggagcgct ccggccgcgg gcttccccgc cgtcatgtaa cggttttgta aaaagtaacg
     2881 gagcgagcgg gcggacgtgc tgacgtgcat gcttcgaaga tgcatttcaa ccgtacggtg
     2941 ctatagtttc gccgcgcaga atcggccgga cggccgggac cacccatgaa ctcagcaagc
     3001 ggcggagctg ccgaaatggc acgcccggcc gactttattt ggcgctttgg cgaggtcgaa
     3061 ctcgacgggc gcagtctcgg gctcaccgtc ggcggccagc cgacggcgat cgaacccaag
     3121 ccgctggcgc tgctgctcta cttcctcacg catcccggcg aagccgtctc ccgcgacgaa
     3181 ctgctcgagg ccctgtggcc ggggcgcatc gtggtcgagg cggcgctcac caactgcgtg
     3241 cacaagctgc gctccctgat cggcgacgag cgcgcgacga cgatccagac cgtgcgcggc
     3301 tacggctacc gcctgctcgc gcccgatctg caggtcgaaa aattaccgga acacgcgtcg
     3361 tcaccaatca cggctccgac gatgaaggcg ggcgaaacgc cgccgggccg cgcgatgtgg
     3421 cgcctgctgc gctgccttgg aagcagcgat tacggcgaag tctggctcgc gcgccacgtc
     3481 aagctccccg aacagcgggt cttcaagttc gcccgcgcca acgcgacgag cctgacggcg
     3541 ctgcggcgcg aaatcacgct gcaccggctg atgcgcaatt ccttcggtca ctgtgagtgg
     3601 ctggtcaacg tgctcgactg gaatctaacg gacgcgccgt ttttcatcga actcgaatat
     3661 gtgcccggag gaagcatcgg cgactgggcg gcttccggcg gcggcatcgg cgaaatcccg
     3721 cttgcaatgc gtatagatct cgtcgtgcag gcagcgcgcg cgctttccgc cgcgcattcg
     3781 ctcggcgtcc tgcacaagga tctgaagcct gccaacctgc tggtggacac gagcgatcca
     3841 gagcgaccgc gcctgcgcgt aagcgatttc ggcagcgggc ggctgctcga tgacgaacgg
     3901 ctcggggcgc tcaatatcac gcgcctcggc ttcacccaca cgggtcttcg cagcgacacc
     3961 gactcaggca ctctgctcta catggcgccg gaggtgctga gcggccagcc cgcgaccatc
     4021 caggccgata tctatgcact cggcgtaatt ctctatcaga tggtggccgg cgatctcgga
     4081 aaaccgctcg cgccgggctg ggaacatgac atcgaagacg aactcctgcg cgaggacatt
     4141 gcgcgcgccg tcgccggcga tccggagcgg cgccttgcga gcgccgccga acttgccgat
     4201 cgcctcgaag agcttgacgc gcgccgcacg gagcgcgaac gcgaacaccg ccgcgcggag
     4261 gaggcggaaa aaacccgcgt cgctctggtg cgtgcgcgcg cgcgccgcgg tctcctgcag
     4321 gcgttggtcg cgaccttttt cgttgccgcc atcgtgaccg gcctgcttct gtggcaaacc
     4381 gaacgggaac ggtccgcggc acaacgcgag gcgcggctcg cccaatccgt gacgcacttt
     4441 ctgaccgacg atttgctctc ctcggccaat cccatgatcg caggctcgcg cgatgtgacc
     4501 gtgcgccaga ttctcgatgc cgcaagcgca aagctcgacc agcgattcgc gcatcaaccg
     4561 ctggtgaagg cttcgctcga acgcgtaatc ggcaccgcct atgccgcgat ggacgaccgc
     4621 aagcaggccg agccgctgct gctcgcggcc gaacgcgggc tcgccgcgca gctcggcaac
     4681 gccgcgcccg agaccgagga gacgcgcctt gcgctgcgcg atctttatct cgacttgttc
     4741 gacatcaagg gcaccgtcgc cgtgtccaag cgcatcgaca gcgccgaacg cgcggcgggc
     4801 gtgcccaacc ccgaggccga gtacgaggcg cgcgcgatgc tgatcgcgat gccggccatt
     4861 gcccgtgccg gcgcgtactg gctgaccgat ctcgcggcgc caatgaatca gctgtacgta
     4921 gaagcgggaa agcagctcgg tttgaacaac cgcgcaaccg cgcgcatgct ctggtacacg
     4981 ggcgtcacgc tctcctggga acatcgctat cgcgaggcgg cgcccattga tcgcgaggtc
     5041 ctgcgtcaat tccaggtgat ttacggtccc gatcatccgc gcacgctgga ggccgcgatc
     5101 tatctcgcac aggcgctcga tcataccggc cgcgccgcgc aagcgcgccc gctgcttgtg
     5161 aaaatgattg cagacttcga gcatacactc ggtccggacc acgtcttcgt tctcaactcc
     5221 cgccgctttc tcgcggaaag cgatctgctg acgggcaaac ccgaggccgc ggtcaaggaa
     5281 atgcgtggcg tatatgcgcg gcgcaagact ctgctcggtg aaacgagccg gctcgccgaa
     5341 gccgacgcac tccacctcgg gaagatactc gcggctgccg gacggcccgc tgaggcgctt
     5401 cctattctcg agcgactcct ccaccttcag gaacaggatc tcggcgtcgc ctcgccgcag
     5461 gcaatgctga cacgcgacgc gctcggaaag gtgctcgtcc agtcgcacca agaccgcgat
     5521 gcagaagcac tgctcgagaa aaactatcgc ctcgccgccg cacgactgca cggaggatgg
     5581 tttctcggcg aaactgcctt tcaacttggc aaggtgctcg agcgcaacgg caagcccgac
     5641 cgggcgaaac ccttgctcga agaagcggcg acggtgttta cgaccgcgct cggccccaac
     5701 aatcccatga cggaaatggc cgaacaagaa ctggccagtt cctgaattcg cggcgcatct
     5761 ttctgcgcct tcgtcccgtc ggaagtaacg actgcccggc gcacaacgac gccgaccggg
     5821 aaaatttctc ggcgcagaaa tgccgggcgg cgatcgggaa cgacctccgc ccggcattcg
     5881 ccggcgtgct accagatctt gcagcggtcc ttgttgacca tcgggctgtc ggcgggaacg
     5941 tcgaaggcct gttggaactg cggcatgttc gcgagcgtcc cgttcacccg gtacatagcg
     6001 ggcggatgcg gatcgatcgt caccagccga cgcgattcct cggggcggat gttcatcgcc
     6061 cacacgtggg cagcgccgag gaagaactgc tgatcgggcg tgaagccgtc gatcgtcttc
     6121 gcttccttgt aggccggcga cgcatggaac gcgcgccagg cgagcgtcag gccgccgaag
     6181 tctgcggttg cctcgccggt gacaagcttg ccctgcacgt gcagaccgtt gtccacggtg
     6241 tactgcgaga agtgatccga gatgcaggag gtcgcggcct tgaacttctt caggtcttcc
     6301 ttcgtccacc agttcttgag attgccgtgg ccgtcgaact gcgcgccctg atcgtcgaag
     6361 ccgtgggtca tttcgtgtcc catgacgaag ccgagcgcgc cgtagttcac cgccgccggc
     6421 gcattgggat cgaagaacgg cggctgcaga atgccggccg ggaagttgat gttgttcatg
     6481 gacggatcgt agtaggcgtt gaccgtctgc ggcgtcatga accattcgct ccggtccacc
     6541 ggctttccga tcttggcgag atcccgcttc gtgaggaaca cattggccgc gattacgtta
     6601 tccacatacg ggcctttggt aatcgtgagc ttggaataat cgcgccattt gtcgggatat
     6661 ccgatgcgct ctcccatcat gccgagcttc ttgatcgccg cctcgcgggt cgccggcgtc
     6721 atccacgaca gagttgcaag atcatccttg agcgcgccgc ggatgttgtg cagaatcgcg
     6781 agcacggcct tcttcgagga cggcggaaac tccttttcca cgtagagctt gccgaccgca
     6841 aagccgagcg cgtcgtcttc gctcgccacc acgcgctgcc agcgcgggcg cagctccttg
     6901 gctccggtga gcgcggactg catcttgaag tcttcgtcca cgaagggttt ggagagatac
     6961 ggcgcgaagc tgtcgacgag atgccagcgc agatacgtct tccagtccgc gagcggaacg
     7021 ctcttcagat cgtcgccaag cgccttgaag aaatcgggca tcgcaagatt ggcgctctgg
     7081 agatccggaa cgccgatggc gctgaaatat tcctgccacg agaaattcgg ggtcgcctcg
     7141 tcgagcttgg cgagcgtcat cacgtgatag atggcgtgcg gatcgcgctg ggcgacctgc
     7201 gacatcgaag cctgtgcgag accggtctcg attttcatca ccgtctgcgc ttcggccgcg
     7261 gcctgcgtct tgctgtcgcc gagaagttcg aacatggcgg cgatgtgcgc cacataggca
     7321 tcacgcacct gctggaattt cgcggcctgc gccgtacaat ccgtgtaggc ggcttccgcg
     7381 gccttggcgg cgctcgcgcc atgcgcggcc ggcgaagtac cttccgccgc cgccttgcgg
     7441 tccgccgcgc actggtcctt cgtcttcaga tagtagtcgc gatccggaag accgaggccg
     7501 ccctggaaag cggcgccgat cacctggctc gagtccttga agtcctgcat cgagccgaag
     7561 ccgaacatgg cgttcacgcc catcatctgc aggcgcgcga cttcctgctg gagatcgtcg
     7621 gtgttgttga tcgcggcaat gcgggcgaat tccggcgcga gcggtttcgc gccttccttg
     7681 tcgatgcgtg ccatgtccat gccgctcgca tagaagtcgc ccaccttctg ctgggtactg
     7741 ccgggcttgg cgtctgcctt ggcggcatcc tcgagaattt cccgcacgat cttctcgttg
     7801 cgaatctgca ggagattgaa ggttccccag cgcgagtagg ccgccggaat cggattctgc
     7861 ttctgccagc cgccattggc gtactcgaag aagttggtgg aagggctgac cgccttgtcc
     7921 agccagctca ggtgcagcgc ctcgacttcg gccttgggca cgtcggcctc acccgccgcg
     7981 agcgcgatca gcggggccgc ggccaccgcg gcgatcgcca cgtgtttgaa aaatcgcata
     8041 aaaatctcca tgcaaagaga agggaagctg ctcctccgcc accggtttca cgcggcggga
     8101 agcctgtccc gaggggaaaa acgcgcctac tatacgacat ccgcaccccg tttcggacgc
     8161 tggtcgcacc gcaggcatct cagcgcggga cgcgcagccc gccgggcacg ccgccggggg
     8221 aaagcagaat ctgaaagagc tggtcgctgc gggtgcgaaa gctcgccgcc gaggccatga
     8281 gatagaaccg ccacatgcgc cggaaacgct cgccgtagcg ctcccgaagc tcgggccagg
     8341 cacgctcgaa gttcgcatac cacgacatga gagtcttgtc gtagtcggcg ccgaagttgt
     8401 gcagatcctc gatgacgaag agccgttcga tcgcccgcgt cacctgccgc ggcgacggaa
     8461 tcatgccgtt ggggaaaatg tacttctcga tccagggatc ggtacgggtg agcgacacat
     8521 tgccgtgaat cgcatgcaga aggaagagac cgtcgggctt gagcaggcgg cgagcggtcg
     8581 tgaaatatct gcggtaattg cgatggccga catgctcgaa catgccgatg gagacgatgc
     8641 gatcgtaggc gccgcgatga gcacgatagt cttcgacgcg gatttcgacc ggcaatccgg
     8701 cacagagatg ctgcccgtac tcggcctgtt ctttcgagac ggtaatgccg acgaccgaaa
     8761 cgccgtagcg ttcggccgca aatttcgcca tcccgcccca gccgcaaccg atgtcgagga
     8821 gccgcatgcc ggcctcgagc ccgagtttgc ggcaggcgag atcgagcttc gcctcctgag
     8881 cttcgtcgag cgtcgtcgcg tccttccagt aaccgcaact gtaggtcatg cgccggtcga
     8941 gcatgcgttc gaacagatcg ttgccgcgat cgtaatgttc ttttccgacg gtgaaggcgc
     9001 gccgcccgct ctgcagattc acgagctgcc cgagcagcca gcgcgccgcc ttctgcggga
     9061 gggccatgcg ccggtcgagc cgcgcgccga tcacccgcgt gaggagttcg tcgagacgct
     9121 cggcatccca ccagccgtcc atgtaggact cgccgaagcc gagcgaaccg tgggcgaaga
     9181 cccgcgcgaa gagcctcggg tcgtgcacct gaatgtccca gggccgatcg ccgccgatgc
     9241 gcacatcggc aagggcgagc agacgcgcgg ccttgcgctc aagacggccg gccgtcccgg
     9301 aaggagcagt tgttgtcatg tcggcatttt aacggctgcc ttggtttccg atcgcaaagt
     9361 tggcaacgag gacttgtcgc cggcaagctt cgcgtccggc gcaaggcggg agcccgaagg
     9421 tgagggtcgc cccgcgacgg ccgcttgcgg ccggccgctc caaaaaagtc ggggcggccg
     9481 aagccgcccc gacgagcgac gcgcttgcgt cgctctccct ccccactctc gaccccaaca
     9541 tccggttgat gaccagtcgc ccatccaacc cgtgccttgc ggcccggacg gccgatagcg
     9601 cttggacttc gcgtcaatat ccgtcatact gacgccgcga cgcgtaccgg caagacacag
     9661 tattgccgga agatcagaca aaagcctcaa ggcaagatgg ttttttcgtc ggttttgggt
     9721 ggttttttcg tcccgtcggc gcggccgagg agggatcgcc acatatggcg tcggaaaaca
     9781 cgaggagtca cgtcttcggt gccttccgcc tgcaaggcgc ggatcgaagg cttttccacg
     9841 acggggtgga agttcccatc gcccccaagg ccttcgatct tttgcaggtt ctggtggaaa
     9901 atcccggccg cctgctcagc cgcgaggagc tgattcaggc cctctggccg cgaaccatcg
     9961 tcaccgagca caatctcacc tcgacgctct accagctgcg caaggcgctc ggcgacgagg
    10021 gcagcccgcc gcgctacatc gagaccgccc gaggtcacgg ctaccgcttc atcgcgtcgg
    10081 tatcgctcat cgatgcaggc ggcaggccgg tgccggacgg ctcgcgccca acggcaaggt
    10141 acgaacatcc cgcccggcgc ttgcgacggg cggcgctcgc cgcggctgtg gtcgtcgtag
    10201 tggttggggc cgcgatcttc ctgtggcgtt ggaactccgc cgcgaaatcc ggggacgatc
    10261 ggacggcaac gcccgtcatt gccgtgctcc cttttacgaa tctcagcgcc gacaaggcca
    10321 atgcctacct gacccaaggc atccaggaca cgatcctcac gcgcctcgcg gcgatttccg
    10381 gcttgcgcgt catttcccac acttctacgg aatcctacgc cagccgcccg gaaaatctga
    10441 agaccgtcgc gaggcagctc ggtgcatcgg agatcctcga gggaagcgtg cagaaggccg
    10501 gcgaccgcgt gcaggtcaac gtccaactca tcgacgcgcg gaccgacaca cacctctggg
    10561 cgaagaccta cgatcgcgat ctcgaaaacc tgttcggtgt cgagggggag gtcgcggcaa
    10621 atgtgacgca ggcgctgcaa acgaaactgc tgccgacgga aacggcaagc ctgagccgac
    10681 cgcctacgcg cgatgcgcag gcttatcttc tctatctcaa ggccaatcat tacacggacg
    10741 aagcctttta ccggaccaac gcgaaggacc cggcaagcac cgtagccaaa gcgacggcac
    10801 tgtatcagca ggcgatcgcc cgtgatcccc ggttcgcgct cgcctacgcc cgcctatctc
    10861 tgctggacgc cttcgcctac tggttcgaga tcgacccgag tcagatccgc gccgcagcgg
    10921 ccgagttggc ggccaaacgg gcgcttgcgc tcgatccggc gctgccgccg gcgcgcttcg
    10981 cgatgggcta cgtcgaatac tactgccacc gcaattatcc ggcggcgctg cgccaattca
    11041 atctggcgtc cctcgggttg ccgcacaacg ccgccgtcac cggcgccatc gccttcatcc
    11101 accggcgcca gggaaaatgg gacgaggcgc tggacggcct ggccaaggcg gccgcattcg
    11161 atccccgcaa ccctagctgg ttcaccgaaa tggggaacat cctcatggca ctgcgtcgct
    11221 atcccgaggc agtggaaaag ttcgaccaag cgctggcgat cgcgcccgat tcctacgatg
    11281 cggcaggtga ccgggtggcc gcatggttca tggccggcaa gctggcgcag gcgagcaaag
    11341 cgctggccca gattcccccc ggcgtcgacc cgcagggtct cgcttcgatg ctgcgcttcc
    11401 agattgcgtg gctcgcgcgt gctccggatc gtgcgctcgc cgccctcgcc acgggtccga
    11461 cacaaatggc ggggcccgaa actctgatta ccgttccgaa gagtctgctc gaggcgcggg
    11521 tatggacgct gaaaggcgac ccgggcaagg cccgcccgct ctatcaggac gcccgcgaca
    11581 aactcctggc cgagctgcat cggcgtcccg agagtcccga tctctggagc gccgaaggtc
    11641 tcgccgcggc cggattggga gagaaggccg cggcgattcg agccggccgc cgggcggtca
    11701 agctctttcc cccgtcgaaa gatgcgatgg ccggtccagt ctatctcacc aacctcgcgg
    11761 agatctatgc catctcgggc gaggccaagc cggtggtgcc gttgctgcgc cgcttgcttg
    11821 cgatgcccgc gggtgcttcg gtgtcgatcc cggtcctgcg aatgaatccc atctgggatc
    11881 ccgtccgcaa ggatccgggc ttccaagcgc tccttcgccg gcgtggcgga gcggcgcccg
    11941 ccgttccggc aagcgtcgcg gtcgcgatcg gcacgggcgt caagcgataa gggcgcacca
    12001 gggcccgagc gagttggtca ggatatgcgg cgcggaggag agtcgcccga agcagcggcg
    12061 ccgcccgcgc ccgaggtgac cgcgggtagc ttgagcgggc ggatgccgag ttcggcaagc
    12121 tggcgcgcgt ccgcctcggc gggcgcgccc gtcatcggat cctgcgccgt ctgcgttttc
    12181 gggaaagcga tcacctcgcg aatgttgggc gcaccggtca tcagcatcac cagccgatcg
    12241 agcccgaagg cgatgccgcc gtgaggcggt gcgccataac gcaaggcttc ggtcagaaag
    12301 ccaaacttct cttcggcttc gatgtcggcg atgccgagca cggcaaacac ggcacgctgc
    12361 atctccgggg tgtgaatgcg aagcgaaccg ccaccgatct catagccgtt caggaccacg
    12421 tcgtaggcgc gggacaggag ttgtcccggc gcgcccgcca gcgccgccgg gtcggattcg
    12481 gacggcgcgg tgaatggatg gtgcagcgcc tgccagcgtt tctcttcctc gttccattcg
    12541 aacatcggga aatccaccac ccagagcggc gcccaacccg cacgctggag accccgctct
    12601 tcggcaagcc gcacgcgcaa ggcggcgagc gattcgttca cgatcttcgc cgccccggcg
    12661 ccgaagaaga gaatgtcgcc gttcgccgcg ccgctgcgat cgagaatcgc ggtgagtgtt
    12721 tcctccgcga ggaacttcac gatcggcgat tgcaggccct ctcgccccgc cgcacgatcc
    12781 gccaccttga tccaggcgag tcccttcgct ccgcagcggg aaacgagttc ggtatagccg
    12841 tcgattgcgc cgcggctgag atcgcctccg cccggaaggc gcagcgccgc gacgcgcccc
    12901 gccgggtcgc gcgcgggtgc ggagaaaacc ttgaattcgg aatcgacgac cagatctgcg
    12961 atctcgacca gctcgagggg attgcgcaga tccgggcgat ccgttccgaa tcgcgcgacc
    13021 gcatccgcgt agctcaggcg cgggaactcg ccgagatcca ctccgagttc gcccgcgaag
    13081 agttcgcgca gcatgcgctc catgagcgcc gtgatcgcgt gctcgtcgag aaacgaggtc
    13141 tcgatgtcga gctgggtaaa ctcgggttga cggtcggcgc gcagatcctc gtcgcggaaa
    13201 caacgcgcga tctggtaata gcgttcgagc cctcccagca tcaggagttg cttgaagagt
    13261 tgcggcgact gcggcagcgc aaaaaacttg ccgcgatggg tgcggctcgg aacgagatag
    13321 tcgcgcgcac cttcgggagt tgccttggtg aggcagggcg tttcgatttc gatgaagcct
    13381 tcgtcgtcga gaaaagcgcg gagcctgcgg atgatgcgat ggcgcgcgcg cagccgctcc
    13441 tgcatcagcg gccggcgcag atcgagatag cggtagcgca ggcgcagatc ctcgccgact
    13501 tcttcctcgt ccggggtgaa cggcggggtc gcggcggcgg cgaggatctt gacctcggcg
    13561 gccagcacct cgacctcgcc gctcgcgagc gccgggtttt cggttcccgc ggggcggggg
    13621 cgcacgcgcc ctgtgacttc gatgacccat tcgccgcgca ggcgcccggc gcgggcgaaa
    13681 gcctcgtccg cctcgggatc gaagacgatc tgtaccagcc cggagcgctc ccgcaggtcg
    13741 acgaagatga cgccgccatg atcgcgccgc cgatgcaccc agccggccaa ggtcacggtg
    13801 cggtcaatca gcgaagcgtt gagatcggcg caaaaatgcg agcgcaagaa acttctcagc
    13861 ggttgggagg gaaccaatcg ggccgctcgg aggcccgccg gtgtcggcat tgtatcggcc
    13921 gccgggcgag gcggcaaggc gatcgcccag cccggaacgc cggcgccgct cagcgcaagg
    13981 cggcgcagac ttggcagttc tgccgaaagt cccgatcgcc gcgcgccgaa gcccgccgcg
    14041 gataatgtga atgaggcagg ggagtcccgc cgttcggctt cgacgacgtg gggaaaaaca
    14101 aagaagtccg gcttgccgaa ccgcggcgag atcctcagac cgcggtct
//
LOCUS       DASUJG010000132         4179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_281502, whole genome shotgun sequence.
ACCESSION   DASUJG010000132 DASUJG010000000
VERSION     DASUJG010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281502"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1752
                     /locus_tag="VFX38_02705"
     CDS             <1..1752
                     /locus_tag="VFX38_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002627055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEX5313802.1"
                     /translation="TGVAYLFSLVALIVPGFLPAAFLGHGGAPPLYFESAAVIVTLVL
                     VGQVLELRAREKTSGAIRALLDLAPPTARRIADDGSERDVALAEVAVGDRLRVRPGEK
                     IPVDGEVLEGRSNVDESMLSGEPLPVQKLAGAMLVGGSVNGNAGLVMRATRVGSETLL
                     ARIVAQVAAAQRSRAPIQGLADRVAKVFVPAVIASAVIAFIAWAFASSGTGGFVFALV
                     IAISVLIIACPCALGLATPMAIMVGVGRGAGSGILVRDAEALERMATVDTLVVDKTGT
                     LTEGKPRLDRLVAAGGFDEREVLALAAGLEAASEHPLAAAILAGASERGVALARVEQF
                     ESVPGRGVRGTVGARAVLVGNAAFLKQSGIDTAALNERAEALADSAATVMRVAVDGRA
                     AGLIAVRDPIKASSRAAVSALRKAGLRIIVATGDGVRAANAVAAELGIAEVVAEALPE
                     KKLELIERLKSEGRIVAMAGDGVNDAPALAAAAVGIAMGTGTDVAMESAGLTLLGGDL
                     RALARARRLAQATLGNVRENLFLAFVYNAAGIPIAAGLLYPFTGWILSPMIAAAAMSL
                     SSVSVIANALRLRKLAL"
     gene            1851..2855
                     /gene="holA"
                     /locus_tag="VFX38_02710"
     CDS             1851..2855
                     /gene="holA"
                     /locus_tag="VFX38_02710"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX5313803.1"
                     /translation="MKLSASTLPKALASGLRPLYVILGEEPLTAREAGDAVRATARAA
                     GFAVRIPLFAEPGFDWGLLHTEGATGSLFGDRRLVDLKLPGGKPDAEGARALAAWFAA
                     PPADVLLLVTAIGAERKAAQTAWAKAAEKAGVLVECRPIPATDLPGWIKERLRQRNLA
                     LPSEAIGLIADYAQGNPSAAAQAIERLLLLAPIGDTDLENVREAIVDEARYGTFECVD
                     AALAGDAAGTLHMLRRLAEAGTAPPQLLWVIARDLRLLASWRWASELGGPRPQVWDSR
                     RSLIAAAARRRQADSWQSLLVAAAEADRCIKGRAPGNAWTRLERLLLTIAGVAMPEAA
                     "
     gene            2869..3507
                     /gene="nadD"
                     /locus_tag="VFX38_02715"
     CDS             2869..3507
                     /gene="nadD"
                     /locus_tag="VFX38_02715"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018870643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX5313804.1"
                     /translation="MSRIGIFGGTFDPIHFGHLRPALELRQSLGLEELRFLPCRMPPH
                     RRAPGAPPPLRCEMVARAIAGVPGFVLDRRELDRSGPSYTIDTLREVRREMPTAQLVL
                     LLGSDAFLGLPGWHDWERLFEFAHFAVTHRPGRRPGGGAPFTTLMTERLVEEPAQLWR
                     EASGRILFVEVTQLEISATAVRETVAAGGDIRYLVPEVVREFIEETGCYQQA"
     gene            3467..3862
                     /gene="rsfS"
                     /locus_tag="VFX38_02720"
     CDS             3467..3862
                     /gene="rsfS"
                     /locus_tag="VFX38_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX5313805.1"
                     /translation="MSSSKRPAVINKPEFADDPRVLAGAVVAALEEVKGVAITTIDVR
                     GKTPLTDFLVIASGNSERHLLTLRDAVLECAAGLGIRPLGVEGAGAAEWILIDLVDCV
                     VHLMRPAARAFYDLERLWSVGPEAAPPSS"
     gene            3862..>4179
                     /locus_tag="VFX38_02725"
     CDS             3862..>4179
                     /locus_tag="VFX38_02725"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEX5313806.1"
                     /translation="MRLRLLAVARRPPAWVESALSDYARRLPRVLHFSAPLVPPAPGH
                     DRNPARAFRAEGERLLANITRDETVVVLDEHGESWTSVELAARLEAWQAAGADLAFLV
                     GGAD"
BASE COUNT          588 a         1384 c         1494 g          713 t
ORIGIN      
        1 accggcgtgg cctatctctt cagcctcgtc gcgctgattg ttcccggctt tcttcccgcg
       61 gcatttctcg ggcacggcgg cgcgccaccc ttgtacttcg aaagcgcggc ggtcatcgtg
      121 acgctggtac tcgtcggcca ggttctcgaa ttgcgtgcgc gcgagaaaac ctcgggcgcg
      181 attcgcgcgc tcctcgatct cgcgccgccg acggcgcggc gcattgccga cgatggctcg
      241 gagcgcgacg tggcgctcgc cgaggtcgcg gtcggcgatc gcttgcgcgt gcgcccgggc
      301 gagaagattc cggtagacgg ggaggttctc gaagggcgtt cgaacgtgga cgaatccatg
      361 ctgagcggcg agccgctgcc cgtgcaaaag ctggcagggg ccatgctcgt cggcggaagc
      421 gtgaacggca atgcgggatt ggtcatgcgc gcgacgcggg taggctccga aaccttgctc
      481 gcgaggatcg tcgcgcaggt ggccgcggca cagcgcagtc gcgcgccgat ccagggtctc
      541 gccgaccggg tggcgaaggt cttcgtaccg gcggtgatcg cgagcgccgt catcgccttt
      601 atcgcctggg cgttcgcgtc atccggaacc ggcggtttcg tctttgcgct cgtgatcgcg
      661 atttcggtcc tcatcatcgc ctgtccctgt gcgctgggcc tcgcgacacc gatggcgatc
      721 atggtcggcg tcgggcgcgg cgccggaagc ggaattctgg tgcgcgacgc cgaggcgctc
      781 gagcgcatgg cgacggtcga tacgctggtc gtcgacaaga ccggcacgct caccgaaggg
      841 aagccgcgac tcgatcggct ggtcgcggcc gggggattcg acgagcgcga ggttctcgca
      901 ctggcagccg ggctcgaagc ggcgagcgag catccgctcg ccgcggcgat tctcgcggga
      961 gcgagcgagc gcggtgttgc gctcgcgcgt gtcgagcaat tcgaatccgt tccgggccgg
     1021 ggggtgcgcg gaacggtcgg cgcgcgcgcc gtgctggtcg gcaatgcggc attcctgaag
     1081 caaagcggta tcgatacggc cgcgttgaac gaacgtgccg aagcgctcgc cgacagcgct
     1141 gcaaccgtga tgcgggtcgc agtggacggg cgcgcggccg ggctgatcgc ggtccgcgat
     1201 ccgatcaagg cgagcagccg cgcggcggta tcggcgctgc gcaaagcggg cctgcggatc
     1261 atcgtcgcga ccggggatgg cgtgcgcgcc gcaaacgccg tcgcggcgga actcggaatc
     1321 gcggaggtcg tcgccgaagc cttgcccgaa aagaaactcg aactgatcga gcgcctgaaa
     1381 tccgaagggc gaatcgtcgc catggccggc gacggcgtaa acgatgctcc ggctctcgcc
     1441 gccgccgccg tcggcatcgc catgggcacc ggcaccgatg tggcgatgga aagcgcgggg
     1501 ctcacgctcc tcgggggcga tctgcgcgcg ctcgcccgcg cccgccgcct cgcacaggcg
     1561 accctcggga atgtgcgcga gaatctgttc ctcgcattcg tctacaacgc ggccggcatt
     1621 ccgatcgcag cagggctgct ttatcccttc accggctgga ttctctcgcc gatgatcgcc
     1681 gccgccgcca tgagtctcag ctcggtatcg gttatagcga acgccctgcg gctgcgaaag
     1741 ctcgcgctct gacagcgccg ggagacgcgc agccgctgcc agacaccggg cgagacaaat
     1801 cgtcggatgc gctatagtga aaccatggtg aggccctcgc ccatgagtct ttgaagctct
     1861 cggcttcgac cttgcccaag gcgctcgcct cgggtcttcg ccccctgtac gtaatcctcg
     1921 gtgaggagcc gctcacggct cgggaagccg gcgacgccgt gcgcgcgacg gcgcgggcgg
     1981 cgggctttgc ggtgcgcatt ccgctgtttg cggagccagg attcgattgg ggtctgctgc
     2041 acacggaggg cgcgaccggt tcgctgttcg gcgatcggcg tctcgtcgac ctcaagcttc
     2101 cgggcggcaa gcccgatgcc gaaggcgccc gcgcgctggc cgcctggttc gcggcgccgc
     2161 ctgccgacgt cttgctcctc gtcaccgcca tcggcgccga acgcaaggcg gcgcagaccg
     2221 cctgggccaa ggcggccgag aaggcgggag tgctggtcga atgtcgcccg atccccgcca
     2281 cggatttgcc cggctggatc aaggagcgtc tgcggcagcg gaacctggcg ttgccctccg
     2341 aggcgatcgg cctgatcgcc gattacgcgc agggcaatcc ctccgcggcg gcgcaggcga
     2401 tcgaacgcct gctgctcctc gcgccgatcg gggacacgga tctcgagaac gtgcgcgagg
     2461 cgatcgtcga tgaagcccgc tacggcacct tcgagtgcgt ggatgcggcg ctcgcgggcg
     2521 atgctgccgg gacgctgcac atgctgcgcc gcctcgccga ggcgggcacc gccccgccgc
     2581 aactcctgtg ggtgatcgcg cgagatctgc gcctgcttgc ctcctggcgc tgggcttcgg
     2641 agctgggcgg ccctcgtccg caggtgtggg attcgcgccg gagcctgatc gccgcggcgg
     2701 cgcgccggcg gcaggccgat agctggcaat cgctgctggt ggctgcggcc gaagccgacc
     2761 gctgcatcaa ggggcgggcg ccgggaaatg cctggactcg cctcgaacgt ttgctgctga
     2821 cgatcgccgg cgtcgccatg cccgaagccg catgagcgtg tcgcgcgctt gagccgcatc
     2881 ggcattttcg gcggcacctt cgatccgatt catttcggtc atttgcgacc ggccctcgaa
     2941 ttgcggcagt cgctggggct cgaagagctg cgtttccttc cctgtcgcat gccgccgcat
     3001 cggcgtgcgc cgggtgcgcc gccgccgttg cgctgcgaga tggttgcgcg tgcgatcgcc
     3061 ggcgtgcccg gcttcgttct cgaccggcgc gagctcgatc ggagtggtcc gtcctacacg
     3121 atcgacacgc ttcgcgaggt ccgccgcgag atgcccaccg cgcaactcgt gctgttgctc
     3181 ggctccgacg cctttctcgg cctgccgggc tggcacgact gggagcggct gttcgaattc
     3241 gcgcattttg cagtgaccca tcgcccgggg cgcaggccgg gcggtggtgc gccgttcacg
     3301 accctgatga cggagcgact ggtcgaggag ccggcgcaat tgtggaggga ggcgagcggg
     3361 cgcatcttgt ttgtggaggt gacgcaactg gaaatatccg cgaccgcggt gcgcgaaacc
     3421 gtcgcggcag gcggcgacat ccgttatctg gtaccggagg tagtgcgtga gttcatcgaa
     3481 gagaccggct gttatcaaca agcctgaatt cgcggacgat ccgcgcgttc tcgccggggc
     3541 cgtcgtcgcg gcgctcgaag aggtcaaggg ggtcgcgatc acgacgatcg atgtccgcgg
     3601 caagaccccg cttaccgact ttctggtgat cgcaagcggc aactccgagc gccatctgct
     3661 cacacttcgc gatgcggtac tcgaatgcgc ggcagggctg ggcatccgac cgctcggggt
     3721 cgagggcgcg ggcgcggcgg agtggatcct gatcgacctg gtggactgcg tggtgcattt
     3781 gatgcgcccc gccgcgcgcg cgttttatga tctcgagcgg ctgtggtccg taggacccga
     3841 agccgcgcct ccttcttcct gatgcgtctg cgcctgctcg cggtcgcacg gcgaccgccg
     3901 gcctgggtcg agtcggcact ctcggattac gcgaggcgcc tgccccgcgt cctgcatttt
     3961 tcggcgcctc tcgtgccgcc cgctcccggt cacgatcgaa atcctgcgcg cgcgttccgc
     4021 gcggaagggg aacgactgct cgcgaacatc acgcgcgacg agaccgtggt cgtgctcgac
     4081 gaacacggcg agtcctggac gagcgtcgaa ctggccgcgc gtctcgaggc ctggcaggcg
     4141 gcgggcgcag acctcgcctt tctcgtcggc ggtgcggac
//
LOCUS       DASUJG010000133         6220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_893270, whole genome shotgun sequence.
ACCESSION   DASUJG010000133 DASUJG010000000
VERSION     DASUJG010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6220
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893270"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..1541)
                     /gene="guaB"
                     /locus_tag="VFX38_02730"
     CDS             complement(78..1541)
                     /gene="guaB"
                     /locus_tag="VFX38_02730"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX5313807.1"
                     /translation="MQISREALTFDDVLLLPGYSEVLPREADLATRLTRQIALNVPLV
                     SAAMDTVTEARLAIVLAQEGGIGIIHKNMSPEEQARQVRLVKKFESGVVTDPITVGPA
                     TTIGEVIELRRQHGISGLPVVDGSRLAGIVTSRDLRFEERLGEPVASVMTPAERLVTV
                     GEGATREEVIDKLQRNRIEKVLIVNERFELRGMITVRDINKAKDFPRSAKDEAGRLRV
                     GAAAGVGVQALERVDALAAAGVDVLVLDTAHGHSRGVLDMVRQVRKRHPDVSLVAGNI
                     VTAAAAEALVEAGVDGVKVGVGPGSICTTRVVTGVGVPQISAIRDVAAALAKSDVPLI
                     ADGGVRYSGDFAKAIAAGAHTVMIGSLFAGTEEAPGEVELFQGRSYKSYRGMGSLGAM
                     SQRHGSGDRYFQENSDLDKLVPEGIEGRVPYKGSVSAIVHQLIGGLRASMGYTGSGDI
                     EAMRTRTEFVRVTLAGMRESHVHDVAITKEAPNYRLE"
     gene            1644..1730
                     /locus_tag="VFX38_02735"
     tRNA            1644..1730
                     /locus_tag="VFX38_02735"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1678..1680,aa:Leu,seq:caa)
     gene            1744..2403
                     /locus_tag="VFX38_02740"
     CDS             1744..2403
                     /locus_tag="VFX38_02740"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HEX5313808.1"
                     /translation="MNETERYSETLEQHFPITVLERRLHDVETLNEKLFAWIRDMAAR
                     YGAGAENAAKSASISTQGGYQTSRRTNVFELDRPEIRSLRDRHVLPAVHRYLQTVFGE
                     QARSFNPTLVGWSNLLAGGAWQSPHMHPTENNLASGVYYVHLPPLEPPEGCIEFLNPH
                     PISVHHGFNTTRRIVPEEGMLLLFPPFYLHYVHPFRGDGERAIIAFDVLARRDVVHLV
                     L"
     gene            2411..3448
                     /locus_tag="VFX38_02745"
     CDS             2411..3448
                     /locus_tag="VFX38_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313809.1"
                     /translation="MNESAPYCWQRPQAWLYALDLARDQAWFLDLDREVLHRSAFLDE
                     RIATDVRKRHEIPIGELLAEPIRGAASAPAFLLHTAFCCSTLLARSLDLPGRSLVLRE
                     PAPLLELADLARGLAVSSREPAQLLPMTLDLLSRPFGAAEHVIVKPTNLVNNLATHIG
                     TLRPGSRFLLLYDDLEAFLLAVLKRPRESERGIRQFLARFLADPAGRAWTAARRAVPQ
                     QLGECAALAWGLQIHSLQAWLAVETTARVRILRAEKLLADPVAAIEASANWFGLEMRK
                     DEVQNVIAGPLWQRHAKYPAVAYTPERRREEQALARRLLAQPLAAALAWADAALEMRN
                     ANFPSFLRLLA"
     gene            3480..4832
                     /locus_tag="VFX38_02750"
     CDS             3480..4832
                     /locus_tag="VFX38_02750"
                     /inference="COORDINATES: protein motif:HMM:NF016974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEX5313810.1"
                     /translation="MNEPEKLPPIAELPDQLRVLQQSGAELARRGLHEDAERIFRELL
                     KAAPRHLPALHYLAGRALARGDLDEAQNFLERAIRINPDTAMPHQNLGIVLRARGFPE
                     GALLAFDTALRLRPDLTMAWVQRGDVLQALGRREEAIAAYQRAENLSGDLRALLRAAG
                     DTPRVRESVRRAARHLARARLAAVSEVLAPLRAAHPPPELDRVEQTIRILCRAARPVY
                     EDPLQRPAYAYVPGLPAQPFFARNRFPFLASLERESDAIRAEMFAVLAQPEELRPYVE
                     IAQGLERQWKELNHSPAWSAFHLYRNGERVAAHCARCPATTAAVEALPLVRMQGHAPE
                     IFFSILRPGTHIPPHFGIANYKLAVHLPLVVPPQCAIRVGEETQGWTPGKCLIFDDSF
                     EHEAWNRSAELRVVLIFEVWNPALGEAEREALSLAHSALDRLRRRNAKLAQNPPKRSD
                     "
     gene            complement(4912..5493)
                     /locus_tag="VFX38_02755"
     CDS             complement(4912..5493)
                     /locus_tag="VFX38_02755"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX5313811.1"
                     /translation="MAETGTVRRATIDALPAIVIDGLMPAAEAARLFQWLSGQPFART
                     EFAREDTRAFLHWVCKMSIEECARLPLYEMTLRMVEAHFPERGRQRCYRAYCNSASYG
                     DMLFTHTDTQPGVEGLTALWFIAPRWELEWGGETILFNSAGDAEFVVSPWPGRLLLFD
                     GRIRHAGRPPSRVCVIPRLTFALKLEAAPRERT"
     gene            5562..>6220
                     /locus_tag="VFX38_02760"
     CDS             5562..>6220
                     /locus_tag="VFX38_02760"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HEX5313812.1"
                     /translation="MPVAPIQPYFAVPVASVLLAHGNELNDALRARLLEWERDEGVRT
                     SVPTEVPKHAVYESDFSLFYRKDALIAELARLCLGTLGDLIMRINGYSAAEMQNLRIY
                     HHSWYHITRYGGYTSGHNHPMASWSGVYCVAPGEELSDRPDSGVLRLYDPRVNSLMYL
                     DPGNAHFGPPYGAGSLPMRLRPGELVLFPSYLRHEVAPFWGRDDRITVAFNAWVREAG
                     QA"
BASE COUNT         1013 a         2181 c         1932 g         1094 t
ORIGIN      
        1 cgggtaccga gaggaaaaag gcatgagccg aatgctgcgc acggtgccgt tcgaacttgc
       61 agcccgttgg cgccttccta ttccagccgg taattgggcg cctccttggt gatcgcgacg
      121 tcgtgcacat ggctttcgcg catgccggcc agcgtcacgc gaacgaactc ggttcgggtg
      181 cgcatcgcct cgatatcccc cgagccggtg tagcccatgc tcgcgcgcag gcctccgatc
      241 agttgatgca cgatcgccga gacgctgccc ttgtagggaa cgcggccctc gatgccctcc
      301 ggcaccagtt tgtccagatc ggagttttcc tggaagtagc gatcgccgga gccgtggcgc
      361 tgactcatgg cgccgagcga tcccatgccg cggtaggact tgtaggatcg cccctgaaac
      421 agctcgacct cgccgggcgc ctcttcggtg ccggcaaaga gactgccgat catgaccgtg
      481 tgcgcgccgg ccgcgatggc cttggcgaag tcgcccgaat agcgcacgcc gccgtcggcg
      541 atgagtggca cgtcgctctt cgcgagtgcc gccgcgacgt cgcggatcgc gctgatttgc
      601 ggtaccccaa cgcccgtcac gacccgcgtg gtgcagatcg aaccggggcc gaccccgacc
      661 ttgacgccgt ccacgcccgc ctcgaccagg gcttcggcgg cggcggcggt gacgatgttt
      721 ccggctacga gcgagacatc gggatggcgc ttgcgcacct gccgcaccat gtcgagcacg
      781 ccgcgggaat gcccgtgggc ggtatcgagc acgaggacat ccaccccggc ggccgcgagc
      841 gcatccacgc gctcgagcgc ctggacgccg acgccggccg ccgcgccgac ccgcagacgc
      901 ccggcctcgt ccttcgcgga acgcggaaaa tccttcgcct tgttgatgtc gcgcaccgtg
      961 atcatgccgc gaagctcgaa gcgctcgttg acgatcagca ccttttcgat gcgattgcgc
     1021 tggagcttgt cgatgacttc ttcgcgcgtc gcgccctcgc ccacggtgac cagacgttcg
     1081 gccggggtca tgacgctcgc gaccggctcg ccgagccgct cctcgaatcg aagatcgcgg
     1141 ctggtgacga tgccggcgag acgcgacccg tccacgaccg gcaatcccga gatcccgtgc
     1201 tggcggcgca gttcgatgac ctcgccgatg gtggtcgcgg gcccgaccgt gatcgggtcg
     1261 gtaacgacgc ctgattcgaa cttcttgacg agccgcacct gcctcgcctg ctcttcggga
     1321 ctcatgttct tgtggatgat gccgatgccg ccttcctgcg cgagcacgat ggcaaggcgc
     1381 gcctccgtca cggtgtccat ggcggcggag acgagcggaa cgttgagtgc gatctggcgc
     1441 gtgaggcggg tcgcgaggtc ggcttcccgc gggagcacct cggagtatcc cggcagcagc
     1501 aggacgtcgt cgaaggtgag tgcttcccgg gaaatctgca tggcctgcgc cgcgggagta
     1561 gccgtgtatt atacgacggc gaggcggccc tgcggcccgg ctcgccccga tgcggcgctc
     1621 tccgctacac tgttgcctcc ccggccggga tggcggaact ggtagacgcg ccggactcaa
     1681 aatccggtgg tggcgacacc gtgggagttc gattctccct cccggcacca agcgagcgca
     1741 gccatgaacg aaaccgagcg ctacagcgag accctcgagc agcattttcc gatcacggtt
     1801 ctcgaacgcc gtcttcacga tgtcgaaacc ctgaacgaaa aactgttcgc ctggatccga
     1861 gacatggcgg cgcgctacgg cgcgggcgcg gagaacgcgg cgaaaagcgc cagcatctcg
     1921 acccagggcg gctatcagac ttcgcgccgc acgaacgtgt tcgaactcga ccgccccgag
     1981 attcgcagtc tgcgcgatcg ccatgtgttg cccgcggtac atcgctatct gcaaacggta
     2041 ttcggcgagc aggcgcgctc cttcaatcca accctcgtcg gctggtcaaa cctcctcgcc
     2101 ggaggcgcct ggcagagccc gcatatgcac ccgacggaga acaacctcgc gagcggcgtg
     2161 tattacgtgc atctgccacc gctcgaaccg cccgaaggct gcatcgagtt tctcaatccg
     2221 catccgattt cggtgcatca cggattcaac accacgcggc gcatcgtgcc cgaggaaggc
     2281 atgctgttgc ttttccctcc gttctacctg cactacgttc atcccttccg cggcgatggc
     2341 gagcgcgcga tcattgcctt cgacgtgctc gcacggcggg acgtcgtcca tctcgttctc
     2401 taggccgtcg atgaacgagt ccgcgcctta ttgctggcag cgcccgcaag cctggctcta
     2461 cgcgctcgat ctcgcccgcg accaggcctg gtttctcgac ctcgatcgcg aagtactcca
     2521 tcgctcggca tttctcgacg aacggatcgc gaccgacgtt cgcaagcgcc acgaaatccc
     2581 gatcggcgaa ttgctcgcgg agccgatccg cggggcggcg agcgcccccg cgttcctcct
     2641 gcacacggcc ttctgctgct cgacgctgct tgcgcgcagc ctcgatctgc cgggacgttc
     2701 gctggtgctg cgcgaacctg cgccgctgct cgaactcgcc gatctcgctc gcggcctcgc
     2761 cgtttcttcc cgagagccgg cgcaactcct gccgatgacg ctcgaccttc tgtcccgacc
     2821 cttcggcgcc gccgagcacg tcatcgtcaa accgaccaat ctggtgaaca atctcgccac
     2881 gcacatcggc acattgcggc cgggctcgcg ttttctcctt ctctacgacg atctcgaagc
     2941 ctttctgctc gcggtgctga aacgtccccg tgaaagcgag cgcggcatcc gccaatttct
     3001 ggcgcgtttt ctcgccgacc ccgcgggccg cgcctggacc gcagcgaggc gcgccgtccc
     3061 gcagcaactc ggcgaatgcg ccgcgctcgc ctggggtctg caaatccatt ctctgcaagc
     3121 ctggcttgcc gtggaaacga ccgcccgggt tcgcatcctg cgcgccgaga aactgctcgc
     3181 cgatcccgtt gccgcaatcg aggcgagcgc gaactggttc ggcttggaga tgaggaaaga
     3241 cgaggtgcag aatgtgattg cggggccctt gtggcagcgc catgccaagt atcccgccgt
     3301 cgcatatacg ccggagcggc gccgcgaaga gcaggcgctc gcgcggcgcc tgctcgcgca
     3361 accgctcgcc gccgccctcg cctgggccga tgcggcgctt gaaatgcgca acgcaaattt
     3421 tccgtccttc cttcgcctct tggcataaac tgacgcgcga agattttcgc cgaccccgta
     3481 tgaacgaacc ggaaaaactg cccccgatcg cggagctacc cgatcagttg cgggttctgc
     3541 aacagagcgg cgccgaactc gcacgacgcg ggctgcacga agacgccgag cgcatctttc
     3601 gcgaactgct gaaggcggca ccgcgacatc ttccggcact gcactatctg gcgggccgtg
     3661 cgctcgcccg cggcgatctc gacgaagcgc agaattttct ggagcgggcc atccgcatca
     3721 atcccgacac ggcgatgccg catcagaatc tcggcatcgt cctgcgggca cgcggattcc
     3781 ccgaaggagc cttgctcgcc ttcgacacgg cgctgcgtct gcgcccggat ctcacgatgg
     3841 cctgggtgca acgcggcgac gtactgcagg cgctcggccg gcgcgaggag gcaatcgcgg
     3901 cctatcagcg tgccgaaaat ctgagcggcg acctgcgtgc gctcctgcgt gccgcaggcg
     3961 atacgccgcg agtccgcgag agtgtccggc gggcggctcg ccatctcgca cgcgcccgtc
     4021 tcgccgcggt ctccgaggtg ctcgctccgc tgcgcgccgc gcatccgccg cccgaactcg
     4081 atcgcgtgga gcagaccatc cgcattctct gtcgcgccgc ccggcccgtc tacgaggatc
     4141 cgctgcaacg gcccgcctac gcttacgtac cggggctccc ggcgcagccc ttcttcgcgc
     4201 gcaaccgctt cccttttctt gcctcgctgg agcgcgagag cgacgccatt cgcgccgaaa
     4261 tgttcgccgt gctcgcgcag ccggaagaac tccgccccta tgtcgagatc gcgcagggac
     4321 tcgaacggca gtggaaggaa ctcaatcact cgccggcctg gagcgccttt catctgtatc
     4381 gcaacggcga gcgcgtcgcc gcccattgcg cccgttgtcc ggcgacgacc gccgccgtcg
     4441 aggcgctgcc gctggtgcgc atgcagggac acgctcccga aattttcttc tcgattctgc
     4501 gaccgggaac ccatattccg ccgcacttcg gcatcgccaa ctacaagctc gcggtccatc
     4561 ttccgctggt ggttccgcca cagtgcgcga tccgcgtagg tgaggaaacg cagggctgga
     4621 ctccgggcaa gtgtctgatc ttcgacgaca gcttcgaaca cgaagcctgg aaccggagcg
     4681 cggagctgcg cgtcgtgctg attttcgagg tctggaatcc ggcgctcggc gaagccgagc
     4741 gcgaggccct ttccctcgca cattccgctc tcgatcgcct gcgccgccgg aatgcgaaac
     4801 tcgcgcagaa tcctcccaaa cgttcggatt gatcggttcc ctcttcgccc ccttcggaac
     4861 gaagggggcc gcgtcttcgc ggggggatgt cttcgccccc gcgctggaat gtcaggtgcg
     4921 ttcgcgcggc gcggcttcga gcttgagcgc gaaggtcagg cggggaatga cgcagacccg
     4981 gctcggaggg cgcccggcgt gacggatgcg cccatcgaac aggagaagcc ggcccggcca
     5041 ggggctcacc acgaattcgg catcgccggc ggaattgaaa agtatggtct cgccccccca
     5101 ttcgagttcc cagcgcggcg cgatgaacca gagcgccgtg aggccttcga ccccgggctg
     5161 cgtgtcggtg tgggtgaaga gcatgtcgcc gtagctcgcg gagttgcagt aggcgcgata
     5221 gcagcgttgc cgcccgcgtt ccgggaaatg cgcctccacc atgcgaagcg tcatctcgta
     5281 gagcgggaga cgcgcgcact cctcgatgct catcttgcag acccagtgca gaaacgcgcg
     5341 ggtgtcttcg cgcgcgaatt ccgtgcgcgc gaacggctgg ccgctcagcc actgaaaaag
     5401 ccgcgccgcc tccgccgccg gcatcagacc gtcgatcacg atcgccggca gcgcgtcgat
     5461 cgtcgcgcgc ctcacggtgc cggtctccgc catacccttg ccgatatcgc tgcgggggct
     5521 caaaatagca caaatcccgg gcccctcaac accggacttg catgcctgtt gctccgatcc
     5581 agccctattt cgccgtcccc gtcgcaagcg ttctcctcgc ccatgggaac gaactcaacg
     5641 acgccttgcg cgcgcgcctg ctcgagtggg aacgcgacga aggcgtgcgg accagcgtgc
     5701 cgaccgaagt gcccaaacat gccgtgtacg aaagcgattt ttcgcttttt taccgcaagg
     5761 atgcgctcat cgccgaactt gcacgtctct gtctcggcac cctgggcgac ctcatcatgc
     5821 gcatcaacgg ctacagcgcg gccgagatgc agaatctgcg catatatcat cacagttggt
     5881 atcacatcac ccgctacggc ggttacacgt cgggccacaa tcacccgatg gcatcctggt
     5941 ccggggtcta ttgcgtcgcg cccggcgagg agctttccga ccggcccgac agcggcgtgc
     6001 tgcgtctcta cgatccccgc gtcaattcgt tgatgtatct cgaccccggc aacgcccact
     6061 tcggtccgcc gtacggagcc ggcagcctgc cgatgcgcct gcgccccggc gagctggtgc
     6121 tgttcccctc ctatctgcgc cacgaagtcg cgcctttctg gggccgcgac gatcgcatca
     6181 cggtggcctt caacgcctgg gtgcgggagg cgggccaggc
//
LOCUS       DASUJG010000134         2282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_526229, whole genome shotgun sequence.
ACCESSION   DASUJG010000134 DASUJG010000000
VERSION     DASUJG010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526229"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VFX38_02765"
     CDS             <1..660
                     /locus_tag="VFX38_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017510080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5313813.1"
                     /translation="EGAKVAVCDINLVAIEDTVRTILDTGGNAAGFTIDVTDKESIAR
                     MVTAVMAKWGRIDTLVNNAGIVQDAQFKKMTDEQFERVIDVNLKGVYHCTKAVVDIML
                     EQNSGVILNASSIVGLYGNFGQTNYAATKFGVIGMVKTWARELGRKGIRANAICPGFI
                     ETPILSSIPDKVIKMMEDKVPMGRLGKPEEIANTYAWLASDEASYINGAVIEVSGGCT
                     I"
     gene            complement(767..1606)
                     /locus_tag="VFX38_02770"
     CDS             complement(767..1606)
                     /locus_tag="VFX38_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX5313814.1"
                     /translation="MPDAGVHPMSQPGPAAPRPCGLRAARRAATRVNARLAALGATFF
                     LCGLSSLGFAAGITLPEGPGANLVYARCQTCHDLQYVVDAKGLLPAQWKSTLAGMKDY
                     GLTLSAGDEAAILAYLTAYLGPNPPPAAPPTATSSPSAGAPAARGTSGTSSASVNGRK
                     VFEENCSACHGPEGAGQPGIYPPLAGNPDLAKDSGFPARVVLFGLTGPIEVRSASYSS
                     TMPAFGHLADAEIAAVVNYVRSAWGNASGSAPLDVPGVAALRAHSLTSAEVHAYRSSR
                     GLR"
     gene            complement(1599..>2282)
                     /locus_tag="VFX38_02775"
     CDS             complement(1599..>2282)
                     /locus_tag="VFX38_02775"
                     /inference="COORDINATES: protein motif:HMM:NF015369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5313815.1"
                     /translation="TANGKDVPPVAGGADLIKSYHLTAPQLQNAILALKMNGEPIPTI
                     HGGPVRLIIPGYYGNMNVKWVTDLMLMDRETPSALMQKTYRMPYEPVEPGKFTTQDMT
                     QQNTRPTYGFAIMSVVFAPLAGQTVKAGKVELKGVAWNDGVVPITEVRVSVDQGRSWL
                     ATELDSVDGLTAWHHWQTQVALSAGAHEVWVRAIDAWGRSQPIDGLTTWNPGGWDWHG
                     VDRVAFQVA"
BASE COUNT          411 a          737 c          765 g          369 t
ORIGIN      
        1 gagggcgcga aggtcgccgt ctgcgacatc aacctggtgg cgatcgagga cacggtgcgc
       61 acgatcctcg atacgggcgg caatgctgcc ggcttcacga tcgacgtcac agacaaggaa
      121 agcatcgccc gcatggtgac ggcggtcatg gccaagtggg gccgcatcga cacgctggtc
      181 aacaacgccg gcatcgtcca ggacgcgcag ttcaagaaga tgaccgacga gcagttcgag
      241 cgcgtcatcg acgtcaacct caaaggcgtc taccactgca cgaaagccgt ggtcgacatc
      301 atgctcgagc agaattcggg tgtgatcctc aacgcgtcgt cgatcgtcgg gctctacggc
      361 aacttcggac agaccaacta cgcggcgacg aagttcggcg tgattggcat ggtcaaaacc
      421 tgggcgcgcg aactcggccg caagggtatt cgcgccaacg cgatctgccc gggcttcatc
      481 gagacaccga tcctctcgtc gatcccggac aaggtgatca agatgatgga ggacaaggtc
      541 ccgatggggc gccttggcaa gcctgaagag atcgccaaca cctacgcctg gctcgcttcc
      601 gacgaggcgt cctacatcaa tggcgccgtc atcgaagtct cgggcggctg cactatttag
      661 tagcggcgcg agcggtgagc aggggccccg gcgccagccg gggatgcgaa ccatggcgag
      721 tcgccgcgag cggccgacac gccggcgcac cgtagcgcac cgacgattag cggaggccgc
      781 ggctggagcg gtacgcgtgg acctcggctg aagtcagcga gtgcgcgcgc agcgcggcga
      841 ctcctgggac gtcaagcggt gcggagcctg aggcgtttcc ccaggccgac cgaacatagt
      901 tgaccacggc ggcgatctcg gcatcggcga gatgcccgaa cgcgggcatc gtcgacgagt
      961 agctcgcgct gcgaacctcg atcgggcccg tgagtccgaa cagaacgact cgcgcaggaa
     1021 agccggagtc tttcgcgaga tccgggttgc ccgcgagcgg tggataaatt ccgggctgac
     1081 ccgcgccttc gggaccgtgg caggctgagc aattctcctc gaacaccttt cgaccgttca
     1141 cggacgcgct tgacgtcccc gatgtgcctc gcgccgcggg agcgcccgca gatggcgacg
     1201 acgtcgcggt ggggggtgcg gcgggggggg gattgggtcc gaggtacgcc gtgaggtagg
     1261 cgaggatcgc cgcttcgtcg ccagcactca gcgtgaggcc atagtctttc atcccggcaa
     1321 gggtcgactt ccactgcgcc ggaaggagtc ccttcgcatc gacgacgtac tggagatcgt
     1381 gacaggtctg gcagcgggca tagacaaggt tcgcgccagg accctcgggc agggtgatcc
     1441 cggcggcaaa cccaagcgag gacaacccgc agaggaagaa cgtcgcaccc agggcggcga
     1501 gcctcgcgtt gacgcgcgtt gccgcgcgcc ttgctgcgcg cagtccgcag gggcgcggcg
     1561 ccgccggccc cggctgcgac atcgggtgca cgcctgcgtc aggcaacctg gaacgcgacc
     1621 cgatcgactc cgtgccaatc ccatccaccc ggattccagg tggtcagccc atcgatcggc
     1681 tgcgagcggc cccaggcgtc gatcgcgcgg acccacactt cgtgggctcc cgccgagagc
     1741 gcaacctgag tctgccagtg atgccaggcc gtgaggccgt cgaccgaatc gagctccgtg
     1801 gcgagccacg acctgccctg gtcgaccgag acgcgcacct cggtgatcgg aaccacgccg
     1861 tcgttccagg cgacaccctt cagttcgacc ttgcccgcct tgaccgtctg gccggcgagc
     1921 ggcgcgaaca cgacgctcat gatcgcgaag ccgtaggtcg gcctcgtgtt ctgctgcgtc
     1981 atgtcctgcg tcgtgaactt gcccggttcg accggttcgt acggcatccg gtaggtcttc
     2041 tgcatcagcg cactcggggt ttcgcgatcc atcagcatca gatcggtcac ccacttgacg
     2101 ttcatgttgc cgtagtagcc cgggatgatg aggcgcaccg gaccgccgtg aatcgtcgga
     2161 atcggctcgc cattcatctt gagggcgaga atcgcgttct gcagttgcgg cgccgtgagg
     2221 tggtagctct tgatgagatc cgccccgccg gcgaccggtg gtacatcctt cccgttcgcg
     2281 gt
//
LOCUS       DASUJG010000135         5279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1259974, whole genome shotgun sequence.
ACCESSION   DASUJG010000135 DASUJG010000000
VERSION     DASUJG010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5279
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259974"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VFX38_02780"
     CDS             complement(<1..221)
                     /locus_tag="VFX38_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019337613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5313816.1"
                     /translation="MRIPAALLAAGILALASALAPARADDLDAYVHTTMAPWKVPGLA
                     VAVVKDGQVVLARGYGRRELGKPGKVDADT"
     gene            437..2044
                     /locus_tag="VFX38_02785"
     CDS             437..2044
                     /locus_tag="VFX38_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HEX5313817.1"
                     /translation="MNRFSLPLAASALFLVAASALAAGGPPPGTHGAPASAPASPTQA
                     QKQETHGSVTINGKAIAYDAYAGTLILKNDDQKPIASMSYFAYLKSGVKDPTTRPITF
                     FYNGGPGSSTVWLHMLAFGPKRVVVGNGTLTPPAPYRMVNNDQSLLDATDEVFIDAPG
                     TGFGRVLGKEQGGAGSPSDVYGIDADAQAFDQFIVQFLTEYNRWNSPKFLFGESYGTT
                     RDAVLAYDLEQDNVGLNGVVFLSSILNWSISVDFPQVNPGINISYELGLPSYAATAWY
                     HNKLPHKPADLNAFLKQCERFALGPYAQALDRGDQLDAASKQKIAEALHYYTGLPVAY
                     LLKADLKVSGGQFAHELLGDDDRITGRLDSRFSGPTMDPLGEEAQYDPLDAAIDAPTV
                     SMFNQYVRNTLKFGQNLTYKPQINVFPMWDFKHQPPGAPFALPFTPNVMPDLAAAMKF
                     NPNMKIMLAGGYFDLGTPFYGAIYEMHQLGIPPSLQKNIRYDFFPSGHMVYLNPAAHK
                     GLRDATATFIEQNSGNQPQPQPQPKSQ"
     gene            complement(2121..3512)
                     /gene="fumC"
                     /locus_tag="VFX38_02790"
     CDS             complement(2121..3512)
                     /gene="fumC"
                     /locus_tag="VFX38_02790"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX5313818.1"
                     /translation="MEWREESDSLGKVCVPADKLWGAQTQRSLEHFSIGRDLMPPEMI
                     EAYTILKKAAANANHAGGRLDERRHELIVQVCDEILAGGHRDMFPLHVWMTGSGTQFN
                     MNVNEVISNRCCQLAGTPLGSKKPVHPNDHVNLSQSSNDSFPAAMYMATAIHARSKLR
                     PALEALRDAISAKATEWRDIIKIGRTHMQDATPLTLGQEFSGYAAMLAEDGERLDAAL
                     GDVYKLALGGTAVGTGINAPAGFAEAATEEIARLTAVPFVSAPNKFAVQGAHDALVQF
                     SGTLRTLAVSLYKIGNDVRLLSCGPRAGFNELIIPENEPGSSIMPGKVNPTQTEALTM
                     LAVQVMANDVAVGFGGAGGMLEMNVYKPLMIANILQSIVLLADGMTNFRKFLIEGTRA
                     NEAKIREYVARSLMLVTALTPEIGYDKASEIAHHAHHHGITLREAALALGHVSAEAFD
                     RIVDPKKMVGPRR"
     gene            3660..4325
                     /locus_tag="VFX38_02795"
     CDS             3660..4325
                     /locus_tag="VFX38_02795"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX5313819.1"
                     /translation="MSGIVAAIQGYLEQYGYPVLFVVVLIESFGVPAPGQTLLIAAAL
                     LAATSHLVLAWVLLTAFAGAVIGDSLGYLLGRRGGRKLLIRYGGRIGITKRVVVKVRR
                     KFRRYGGWFVTFARFFDVLRQINGIVAGSARMSFGRFILFNVIGAALWVSLWGFGSYY
                     LGRGLSEWLGDFFHVYATWFLVAVLVFAIVLVSFALWHRARRARKRARRRFARPADES
                     DRR"
     gene            4879..>5279
                     /locus_tag="VFX38_02800"
     CDS             4879..>5279
                     /locus_tag="VFX38_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEX5313820.1"
                     /translation="MKIHIGRAYRLLTGPWRFHVGDDMRWAAPDFDDSRWETADLTPA
                     PGAHDSDVGLTDYVSGWSARGHKGYAGYAWYRMHVAVDAPSDTRIALLAPAYVEDVYQ
                     LFWNGTLIGGAGDFSGETPVIHSTRPQIFRLP"
BASE COUNT          893 a         1729 c         1675 g          982 t
ORIGIN      
        1 gtgtcggcgt ccaccttccc gggcttgccg agttcgcgcc ggccgtagcc gcgggcgagg
       61 acgacttgtc cgtccttgac gacggcgacg gcgagccccg gcaccttcca tggcgccatc
      121 gtcgtgtgca cgtaggcgtc cagatcgtcc gcccgggccg gcgcgagggc cgatgccagg
      181 gcaaggatgc cggcggcaag cagggcggcg gggattctca tcgggcgatt ccgggcggcc
      241 gtcgcccgag tgtacgccat cacggcgcga actccggtcg gcttgacggc attcttgtgt
      301 tgtagtacag tgcaacacac gccgcgcctc cccgcccggg aggcaggccg tcatccccca
      361 tccggcccgg ggctcgggcc gcagcctgac cgggcgatac cgcaagcggt cccggccttg
      421 aacaggagac tttcgaatga accggttttc gctccccctc gcggcgtcgg cactgttcct
      481 tgtcgccgcc tcggctctcg cggccggcgg gccgccgccc ggcacccacg gcgccccggc
      541 gagcgcaccc gcttccccta cgcaggcgca gaagcaggaa acgcatggct cggtcacgat
      601 caacggcaag gcgatcgcct acgatgccta tgcgggaacg ctcatcctca agaacgacga
      661 ccagaagccg atcgcgagca tgtcctactt cgcgtatctg aaaagcggcg tgaaggatcc
      721 cacgacccgc ccgatcacct ttttctacaa cggcggtccg ggttcctcca ccgtctggct
      781 gcacatgctc gccttcgggc ccaagcgcgt ggtggtcgga aacggtaccc tcacgccacc
      841 cgccccctac cgcatggtga acaacgacca gagcctgctc gatgcgaccg acgaggtgtt
      901 catcgacgcg cccggcaccg gcttcggacg ggtgctcggc aaggagcagg gcggtgcggg
      961 ctctccgagc gacgtgtacg gcatcgacgc ggatgcgcag gccttcgatc agttcatcgt
     1021 ccagttcctt accgaataca atcgctggaa ttcgcccaag tttctcttcg gagaaagtta
     1081 cggcacgacc cgcgacgcgg tacttgccta cgatctcgag caggacaacg tgggcttgaa
     1141 cggcgtggtg ttcctgtcct cgattctcaa ctggagcatc agcgtggatt tcccgcaggt
     1201 gaatcccggc atcaatattt cctatgagtt gggtctgccg agttatgccg cgaccgcctg
     1261 gtaccacaac aagctgccgc acaagcccgc agacctgaat gccttcctca agcaatgcga
     1321 gcgtttcgca ctcggtccct acgcgcaggc gctcgatcgc ggcgatcagc tcgatgccgc
     1381 aagcaagcag aagatcgccg aagccctgca ctattacacc ggccttccgg tcgcctacct
     1441 tctcaaggcc gacctcaagg tgtcgggagg acaattcgcg cacgagcttc tcggcgacga
     1501 cgacaggatc accgggcggc tcgattcgcg cttcagcggc ccgacgatgg atccgctcgg
     1561 cgaggaggcg caatacgatc cgctcgatgc ggcgatcgat gcgccgacgg tctcgatgtt
     1621 caaccagtac gtgcgcaaca cgctcaagtt cggccagaac ctgacctaca agccgcagat
     1681 caacgtgttc ccgatgtggg acttcaagca ccagccgccg ggcgcgccct tcgcgctgcc
     1741 gttcaccccg aacgtcatgc ccgatctcgc cgcggcgatg aagttcaatc cgaacatgaa
     1801 gatcatgctg gccggaggct actttgatct cggcacgccg ttttatggcg ccatctatga
     1861 aatgcatcag ctcggaatcc cgccgagcct gcagaaaaac atccgctacg acttcttccc
     1921 ctcggggcac atggtgtatc tcaatccggc cgcccacaag ggcttgcggg atgccaccgc
     1981 caccttcatc gagcaaaatt cggggaatca gccgcagccg cagccgcagc cgaaatcgca
     2041 ataattgcaa taatcgcggt atcgccaagt agtcgtgaag gggccccgcc gggccccttc
     2101 gctttttccg gccgtcggga tcagcgccgc gggccgacca ttttcttcgg gtcaacgatg
     2161 cgatcgaagg cctcggcgct gacatgaccg agggcgagtg ccgcctcgcg cagcgtgatt
     2221 ccgtggtgat gcgcgtgatg cgcgatttcg ctcgccttgt cgtagccgat ttccggggtg
     2281 agcgcggtca cgagcatcaa cgaccgcgcc acgtattcgc ggatcttcgc ctcgttcgcg
     2341 cgggtaccct cgatcagaaa cttgcggaag ttcgtcatgc cgtcggcgag caggacgatg
     2401 gactggagaa tgttcgcgat catgagcggc ttgtacacgt tcatttcgag catgccgccg
     2461 gcgccaccga agcccacggc gacatcgttc gccatcacct gcacggcgag catggtgagc
     2521 gcctcggtct gggttggatt caccttcccc ggcatgatcg aggagcccgg ttcattctcc
     2581 ggaatgatga gttcgttgaa acccgcgcgc gggccgcagg agagcaggcg gacgtcgttg
     2641 ccgatcttgt agagcgaaac ggcgagggtc cggagcgtcc cggaaaattg caccagcgca
     2701 tcgtgcgctc cctggaccgc gaacttgttg ggcgcgctca cgaagggaac agcggtcagg
     2761 cgcgcgattt cttcggtcgc ggcctcggca aagcccgccg gcgcgttgat gccggtgccg
     2821 accgcggtgc cgccgagcgc gagcttgtac acgtcgccga gcgccgcatc gaggcgctcg
     2881 ccgtcctcgg cgagcatggc ggcgtaaccg gaaaattcct gtccgagcgt gagcggggtc
     2941 gcatcctgca tgtgcgtgcg cccgatcttg atgatgtcgc gccattccgt cgccttggcc
     3001 gaaatcgcat cgcgcagcgc ttcgagcgcg gggcgaagtt tcgagcgcgc gtggatcgcc
     3061 gtcgccatgt acatggcggc ggggaagctg tcgttcgagg actgcgagag gttcacatga
     3121 tcgttcggat gcacgggctt cttcgagccg agcggcgtgc ccgccagttg gcagcagcgg
     3181 ttggaaatga cctcgttcac gttcatgttg aactgggtgc cgcttccggt catccacaca
     3241 tggagcggga acatgtcgcg atggccgccc gcgagaatct cgtcgcagac ttgaacgatc
     3301 agctcgtggc ggcgctcgtc gagccgaccg ccggcatgat tggcgttggc ggccgccttc
     3361 ttcaggatcg tgtaggcctc gatcatctcc ggcggcatga gatcgcgccc gatgctgaaa
     3421 tgttcgagcg aacgctgggt ctgcgctccc cagagcttgt cggcgggcac gcacaccttg
     3481 ccgagactgt cggattcttc gcgccactcc atcgtgtgat ttctccttgc cgccacagcg
     3541 gattcgatgc cgcggcgggc ggcgatccac ggtgcccgta gtgtaccgcg gggacggcag
     3601 cagccggaac ccggcgcgcc gctctgatag gatgcttggc ggaaacgggc ggggcgggca
     3661 tgagcggcat cgtcgcggcg attcagggct atctcgaaca gtacggctat cccgtgctgt
     3721 tcgtggtggt tctgatcgaa agcttcggcg tacccgcacc ggggcagacg ctactcatcg
     3781 ccgcggctct cctcgcggcg accagccacc tcgtactcgc ctgggtgctc ctgaccgcct
     3841 tcgcgggcgc cgtgatcggc gacagcctcg gctatcttct cggacggcgc ggcgggcgca
     3901 agcttctcat ccgctatggc gggcggatcg gtattaccaa gcgggtcgtg gtgaaggtgc
     3961 ggcgcaagtt tcgccgctac ggcggctggt tcgtcacttt cgcacgtttc ttcgacgtgc
     4021 ttcgccagat caacggcatc gtcgccggct ccgcgcgtat gtccttcggg cgcttcattc
     4081 tcttcaacgt gatcggtgcc gcgctctggg tttcgctctg gggtttcggc agttactacc
     4141 tcggacgggg actgagtgaa tggctcgggg acttcttcca cgtctacgcg acctggttcc
     4201 tcgtcgcggt tcttgttttc gcaatcgtgc ttgtctcctt cgcgctctgg catcgcgccc
     4261 gccgcgcgcg caagcgcgcg cggcgacgct tcgcccggcc ggccgacgag tccgatcggc
     4321 gctagaccct tgccgttttc cttgttgatg gattgcggaa cgtggagaaa actcggatcg
     4381 gcggcgccgg gtcaagttgg cgacgaggcg gccgccctcg cgtttcgcga gcatgtagcg
     4441 catcaccgag gcgccggtct cgccggggcg agcagcgaag gtgcgatgaa gtctggatga
     4501 aagtcaagtg gcattcgtag cgttctgctg tgctcaaacc gtcgaccgga gcgtcgagcg
     4561 caatgcacaa ctaaagactc cttgtgaggc cgaaaccaac ttgcggagat tttgatgaaa
     4621 gtttgcttct gtagcgcaat cgccctggtc gccgttgcgg gattgacgat cccctgcgcc
     4681 cgcgcggaaa ccaccggttc cgatgcgtac acgagcgcgg cgcatgcact ggctgcgccg
     4741 ggcaggaatg atgacgcata caaatccggc tccggccgcg cgccaaccgc gaccaaggcg
     4801 gttgacgtcg tcgggaataa cgacgcagca gcacctccgg ctcccaacgc ggacgaacaa
     4861 gccggatcgg acccggcgat gaagattcac attggccggg cgtaccgctt gttgacaggt
     4921 ccgtggcgct tccacgtcgg cgacgacatg cgctgggccg cgccggactt cgacgactcg
     4981 cgctgggaaa ccgccgacct cacgcccgct cccggtgcgc acgacagcga cgtgggtctc
     5041 accgactacg tgtcaggttg gagcgcacgc ggtcacaaag gctatgcggg atacgcctgg
     5101 taccgcatgc atgtcgccgt cgatgccccc tccgacaccc gcatcgccct gctcgcgccg
     5161 gcgtacgtcg aggatgtcta tcagctgttc tggaacggga ccttgatcgg cggagcgggt
     5221 gatttttccg gcgagacgcc ggtcatccac agcacccgac cacagatctt tcgcttgcc
//
LOCUS       DASUJG010000136         6754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_37726, whole genome shotgun sequence.
ACCESSION   DASUJG010000136 DASUJG010000000
VERSION     DASUJG010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6754
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37726"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1586)
                     /gene="cydC"
                     /locus_tag="VFX38_02805"
     CDS             complement(<1..1586)
                     /gene="cydC"
                     /locus_tag="VFX38_02805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HEX5313821.1"
                     /translation="MIRLVRLGRSARAAFALGALLALVVILANVALLGLAGWFIAAMA
                     AAGLAGASINYFTPAAGIRAFAILRTVGRYLERLVNHDATLKLLSQLRVWLYARIEPL
                     APAVLARYRKGDVLTRLVADVNTLDNAWLRVFSPALVAFLATVALFVFLLFFSPRVAG
                     VDLALLVVIGAILPFLAQRLGRRPAARAAQTAGRLKAELAEDFAGLAELAVLGAGSAH
                     CKRNDERSRKLAREQDRGFAIEALALGAAVAASGAALALTWLLLAPGSADGRTNALLL
                     PLLMFFVLASTEAVQGLPNAFRALGDTLAAARRVFEIGDETPVVVESPRALERVAGLA
                     LAFDRLRFRYAGQTGWALDDVTFSLPEGAAVAVVGPSGAGKSSLAQLLLRFYDPEAGT
                     IRLGGLPLASYALETLRGQFAYAAQSTRLFSTTIRENLRLGSPQADDETLWNALEAVC
                     LSREVRGFARGLDHFVGAGGMALSAGQARRLALARAWLSPAPILLLDEPTEDLDAASE
                     RTIMKCLTDDRGKRGLLLIT"
     gene            complement(1592..3349)
                     /gene="cydD"
                     /locus_tag="VFX38_02810"
     CDS             complement(1592..3349)
                     /gene="cydD"
                     /locus_tag="VFX38_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HEX5313822.1"
                     /translation="MMTELDRAATAKRWLNREAHAVRRPLAFAIAAGGVNGGLLIAQA
                     LLLAHLVDRTIFGHRPVADQLSPFAMLLGVVVLRALVVCAANRAAVSAGLAIKTGMRA
                     ALVSHIHALGPLWTTARAPGALATTVVDGVEALQDYYAAWLPQASVAVIVPIAILAVV
                     FPVDWISGLILAGTAPLIPLFMVFLGRGAAALNERQWARMARLSAHFYEVLAGLPILK
                     SFGAARTEAKLVARLSEDYRASTMRVLRVAFLSSFALEFLATLSIAMVAVLVGFRLLW
                     GDVTFAAGLAALLLAPEFYLPLRKLGGAYHARLAAVAAAEDIADILAMPVSAPGGTRP
                     FRPHAAFAVAFSKVSFSYAGGAPALCDVTFEIAAGERIAVVGASGGGKSTLARLLLGF
                     AQPCSGRILVDGVSLAELSLADWRRQIAWVPQRAHLFAGTIAENITLGLSDATDEAVV
                     AAAKHAHAHAFIERLPRRYDTLLGEDGAGLSGGEIQRIALARAFLRDAPLVVLDEPTA
                     SLDAESEAEILAAIETLAQGRTVLTIAHRLHTVRTADRIVMLEAGRVVAAGTHAALAE
                     REGPYRRLLGAASPVAQPA"
     gene            3474..3821
                     /locus_tag="VFX38_02815"
     CDS             3474..3821
                     /locus_tag="VFX38_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011400885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX5313823.1"
                     /translation="MDHPLYPESSSHISERRKALAPKQLAAFQAFSREVFADGALPAV
                     TKQLIAVAVAHTTQCPYCIHGHTRGAHRAGASDEQIMEAIWVASEMRAGAAYAHSALA
                     LDELDRLSEKNRS"
     gene            complement(4130..6469)
                     /locus_tag="VFX38_02820"
     CDS             complement(4130..6469)
                     /locus_tag="VFX38_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019349878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX5313824.1"
                     /translation="MSEIAEELIASDHDRTSFYLKWTLFFVGIVTLGALAYGTYVSYQ
                     HAPPQPEQIVAPNGDVLFTGGDIVAGKAAFQQANLGDYGSIYGMGSYFGEDWTAKYLV
                     RLGLLTADSIALQRYGKHYEQLDVGPKAATKEAMQKDLHGLDLRDRRLVVPAPVARAV
                     RQVQNEISRFILTTNAKQGYTRAYSLTQAQARQAADFVIYSSITTVARRPNVDASWTE
                     NWPAEPLVGNSPTNGTFLWTWISIMITFALFGLVVFIFEHWINRDAGKPRVLIREYKP
                     LTPGQRAISLYIVAVAAVFLLQIVAGATMAHSYSERVSFYGIDIGHLVPFQFLRDVHL
                     QGAIVWIGLAWLGSGLFVAPLIAGREGRGQGWMNATLFWVTLAVVAGALIGDYMGIMG
                     WIKQGWFWVGNQGLAYLELGRLWQIGFFAGLFLWSIMMARAIWPRLIDLKDAAVRFWS
                     GRIRLEHLLWASTMNIALLYWFGMIPLTGIEHSFTITDFWRWWVVHLWVEQSFEFFFT
                     MMLAWLLMATGLVSRTLATRTALFDVILIFIGGFLGTGHHLYWVGEPGMWVSVGSVFS
                     FIEVMPLLLLVIEASRQYRSIQAQGKTFPYRLVFLFIWGSAFWNFMGAGVFGGGTLNA
                     PLVNYYEHGTFLTLNHAHTALFGAFGLMALGLVYFCLRYAAGERYKWSNTAGVWAFWL
                     YNGGLVLWIALNFFPIGWPQLEAVYEHGLAYARSLKFYDTTLLWQWLRFPGDVVFAAG
                     AVLMSLDFLVKLRPFLAGRKKRSGVLSPPDREIPAVAGE"
BASE COUNT         1316 a         2137 c         2286 g         1015 t
ORIGIN      
        1 tgcgtgatga ggagcagccc gcgcttgccg cgatcgtcgg tgagacactt catgatcgtg
       61 cgctcgctgg ccgcatcgag gtcttcggta ggttcgtcga gaagcagaat cggcgccggg
      121 ctgagccaag cgcgggcgag cgcgaggcgg cgcgcctgac ctgcggaaag cgccatcccg
      181 cctgcgccga cgaaatgatc gagcccgcga gcgaagccgc gtacttcgcg cgagagacag
      241 accgcctcca gtgcattcca caatgtttcg tcgtccgcct gcggcgatcc caagcgcaga
      301 ttttcccgga tcgtggtgct gaagagtcgc gtcgattgcg cggcgtaggc gaattgtccg
      361 cgcagagttt cgagggcgta actggcaagg ggcaagccgc cgagacgaat cgttcccgcc
      421 tcgggatcgt aaaagcgcag cagcagttgc gcgagcgaac ttttgcccgc gcccgacggg
      481 ccgacgacgg cgacggccgc gccttcgggc aaggaaaagg tcacgtcgtc gagcgcccat
      541 ccggtctgcc cggcgtagcg aaagcgcagc cgatcgaagg cgagtgcgag accggcgacg
      601 cgctccagcg cccgcggcga ctcgacgacg accggcgttt cgtcgccgat ctcgaagacc
      661 cgccgcgccg cggcaagcgt gtcgccgagg gcgcgaaagg cgttcggcaa accctgcacc
      721 gcctcggtgc tcgcgagcac gaaaaacatc agaagcggaa gcagcagggc attcgtgcgt
      781 ccgtccgccg agccgggtgc cagcagcagc caggtgagcg cgagcgctgc gccgctcgcg
      841 gcaacggctg cgccgagcgc caatgcctcg atcgcgaatc cgcgatcctg ctcgcgcgcg
      901 agtttacggc tgcgttcgtc gttgcgcttg caatgcgcgg agcctgcgcc gagtacggcc
      961 agctctgcga gcccggcaaa gtcttccgcg agttcggcct tcagcctgcc ggcggtttgc
     1021 gccgcgcgcg cggcgggacg gcggccgagg cgctgagcaa ggaacggcag gatcgcgccg
     1081 atcacgacga gaagcgcgag gtccacgccg gcgacccgcg gggaaaagaa gagcaaaaag
     1141 acaaagagcg caacggtcgc caggaatgcc acgagcgccg gtgagaaaac ccgcagccag
     1201 gcattgtcga gcgtattcac gtccgcgacg agacgagtga gaacgtcgcc cttgcgatag
     1261 cgtgcgagca ccgccggagc gagcggttcg atgcgcgcgt agagccacac gcgaagttgg
     1321 ctcaggagct tgagcgtcgc gtcgtgattg accagccgtt cgagatagcg cccgacggtg
     1381 cgaagaatcg cgaaggcgcg gatgcccgcc gccggcgtga agtaattgat cgaagcgccg
     1441 gcgaggccgg ccgccgccat cgccgcgatg aaccagcccg cgagcccgag cagggccacg
     1501 ttggcgagga tgacgacgag tgcgagcagg gcgccgagtg cgaacgccgc acgtgcgctg
     1561 cgcccgagac ggacgagtcg aatcaagtcc ttcacgctgg ttgcgcaacg gggctggcgg
     1621 cgcccagcag gcgccggtat ggtccttcgc gttcggcgag cgcggcgtgg gtacctgcgg
     1681 cgacgacgcg gccggcttca agcatcacga tgcgatcggc cgtgcgcacg gtgtgcaaac
     1741 ggtgcgcgat cgtcagaacc gtacgcccct gtgccagcgt ttcgatggcg gcgagaatct
     1801 ccgcctcgct ttcggcgtcg agactcgcgg tcggttcgtc gagcacgacg agcggggcat
     1861 cgcgcagaaa cgcgcgggcg agcgcgatgc gctggatttc tccgccggat agtcccgcgc
     1921 cgtcttcgcc gagcagcgtg tcgtaccgtc gcggcagacg ttcgatgaat gcatgcgcat
     1981 gggcatgttt tgcagcggcg acgacggctt cgtccgttgc atccgataac ccgagcgtga
     2041 tgttttcggc gatcgtgccg gcgaagagat gggcgcgttg cgggacccag gcgatctgcc
     2101 ggcgccagtc ggcgagggaa agttcggcga gcgaaactcc gtccacgagg atgcgaccgg
     2161 agcaaggctg cgcgaagccg agaagcagac gcgcgagcgt gcttttcccg ccgcccgacg
     2221 caccgaccac ggcgatgcgt tcgcccgctg caatctcgaa ggtcacgtcg cagagcgccg
     2281 gcgctccacc cgcataggaa aagctcacct tggagaaggc gacggcaaag gcggcatgcg
     2341 gtcgaaacgg acgtgtacct cccggcgccg aaaccggcat cgcgagaatg tcggcgatgt
     2401 cttcggccgc ggcgacggcc gcaagccgcg cgtgatacgc gccgcccaat ttgcgcagcg
     2461 gcagataaaa ttcgggcgcg agaaggagcg ccgccagtcc cgcggcaaac gttacgtcgc
     2521 cccacaggag gcgaaagccc acgagcaccg cgaccatcgc gatgctcagc gtcgcaagaa
     2581 attccaatgc gaaggacgag agaaaggcga cgcgcaacac gcgcatggtg ctcgctcggt
     2641 aatcttccga caggcgcgcg acgagtttcg cctccgtgcg tgccgcgccg aaactcttga
     2701 ggatcggcag tccggcgagc acctcgtaga aatgtgccga gagccgcgcc atgcgcgccc
     2761 actgccgttc gttgagtgct gccgcgcccc ggccgaggaa caccatgaac aggggaatca
     2821 gcggcgcagt tccggcgagg atcagccccg agatccagtc caccggaaac accacggcaa
     2881 ggatcgcgat cggcacgatg acggcgacgc tcgcctgcgg cagccaggcg gcgtagtaat
     2941 cctgcaaggc ctcgacgccg tccaccacgg tagttgcgag cgcgccgggc gcgcgtgccg
     3001 tcgtccacag ggggccgagc gcgtgaatgt gggagacgag cgcggcgcgc ataccggttt
     3061 tgatcgcgag tcccgcgctc actgcggcac ggttggcggc gcacacgacc agggcgcgca
     3121 gaacgacgac accgagcagc atggcgaaag gggaaagctg gtccgcgacc ggccggtggc
     3181 cgaaaatcgt tcggtcaaca agatgggcga gcagcaacgc ctgcgcgatc agaagtccgc
     3241 cgttcacgcc gccggccgcg atcgcgaatg cgagtggtcg gcgcaccgcg tgtgcttcgc
     3301 ggttcagcca acgttttgcc gttgcggcgc gatcgagctc cgtcatcatc gccgggagtg
     3361 aaaggccccg cgtattgtcg ccgatcgcag cgctgccggt ttgactcgga tcaacgtcgc
     3421 gcgagtcggg gagtgttaca gttgttgtgt tttcacacgt ggaggcaaac gcggtggacc
     3481 atcccctgta tcccgaaagt tcttcccata tttccgaacg gcgcaaggcg ctcgcgccca
     3541 aacagctcgc ggcctttcag gccttcagcc gcgaggtctt tgccgacggg gcgctgccgg
     3601 ccgtgaccaa gcagttgatc gcggtggcgg tggcgcacac gacccagtgc ccgtattgca
     3661 ttcacggaca tacgcgcggc gcgcaccgcg ccggtgcgag cgacgagcag atcatggagg
     3721 cgatctgggt tgcctccgag atgcgcgccg gggccgccta tgcgcattcg gcgctagcgc
     3781 tcgacgaact cgatcgcttg agcgagaaaa atcgatcgta agggcggggc gtgttggtcc
     3841 tccgcacggc caacagggga aatcacgccc ggtttcgggt tttcgatccg ccgggacgcc
     3901 gatccccgga cagctcccca tcctgcgcgg aacgagcgac acggaaacgg gacgcgacgg
     3961 cgcgcgcaaa cgccgcaagc gccgcgacga gccatcctgc gcggaacgag cgacacggaa
     4021 acgggacgac cgcgagagca acggtagcgc cgtcgatcgg caggcggatt tctcacccgc
     4081 cgactcgcaa cgccgatcgc cgaaacacgg gctcggatca tcgggtcatt tattcgcctg
     4141 cgactgccgg gatttcgcga tcgggcgggc tcagcactcc actccgcttc ttgcggccgg
     4201 cgagaaacgg gcgcaacttc acgaggaagt cgagcgacat aagcacggcg cccgcagcga
     4261 aaacgacgtc gccggggaag cgcaaccatt gccacaggag agtcgtgtcg taaaacttca
     4321 agctgcgggc gtaggcgagg ccgtgttcgt aaacggcctc aagctgcggc cagccgatgg
     4381 ggaagaagtt gagcgcaatc cacaacacca gtccgccgtt gtacagccag aaggcccata
     4441 cgccggcggt attgctccac ttgtagcgct cgcctgccgc ataacgcagg cagaagtaca
     4501 ccaggccgag agccatgaga ccgaaggcgc cgaacagggc ggtgtgcgcg tggttcaggg
     4561 taaggaaagt gccatgctcg tagtagttca cgagcggtgc gttgagcgta ccgccgccga
     4621 acacgccggc gcccatgaag ttccagaacg cggagcccca gatgaagaga aagacgaggc
     4681 ggtagggaaa ggtcttgccc tgcgcctgga tgctgcggta ctggcgcgag gcctcgatga
     4741 ccagcagcag cagcggcatg acctcgatga aggaaaacac gctgccgacc gacacccaca
     4801 tgcccggttc gccgacccag tacaggtgat ggccggtgcc gagaaaaccg ccgatgaaga
     4861 tgaggatcac gtcgaagagc gcggtgcgcg tcgcgagcgt gcgcgagacc agcccggttg
     4921 ccatcagcaa ccaggcgagc atcatcgtga agaagaattc gaaggactgc tccacccaca
     4981 ggtgcaccac ccaccagcgc cagaagtcgg tgatggtgaa ggaatgttcg attccggtga
     5041 gcggaatcat gccgaaccag tagaggagag caatgttcat cgtgctcgcc cagagcagat
     5101 gttcgagacg gatgcgcccg ctccagaacc gcacggcggc atccttgaga tcaatcagcc
     5161 gcggccagat ggcgcgcgcc atcatgatgc tccacaggaa cagccccgca aagaagccga
     5221 tctgccacag gcgcccgagt tccagatagg caagaccctg gttgccgacc cagaaccatc
     5281 cctgtttgat ccagcccatg attcccatgt aatcgccgat cagggcaccg gcgaccacgg
     5341 cgagcgtgac ccagaagagt gtcgcgttca tccatccctg tccgcgccct tcgcgcccgg
     5401 cgatgagcgg agccacgaac aagccggagc cgagccaggc gagaccaatc cacacaatgg
     5461 ccccctgcag gtgcacgtcg cgcagaaact ggaagggcac gagatggccg atgtcgatgc
     5521 cgtaaaaact cacccgctcg gagtaggaat gcgccatggt cgccccggcc acgatctgca
     5581 gcagaaacac cgccgcgact gccacgatgt acaaggaaat agcccgttgc ccgggtgtga
     5641 gcggcttgta ttcgcggatc agtacgcgcg gcttccctgc gtcgcggttg atccagtgct
     5701 cgaagatgaa caccaccagc ccgaacaggg cgaaggtgat catgatggaa atccacgtcc
     5761 acagaaaggt gccgttcgtc ggtgagtttc caaccagcgg ttcggcgggc cagttctccg
     5821 tccaggacgc atcgacgttg gggcgccgcg cgacggtcgt gatcgaagag tagattacga
     5881 agtccgccgc ctgacgggcc tgtgcctggg tcaggctgta ggcgcgggta tagccttgct
     5941 tcgcattggt ggtgagaatg aagcgcgaaa tttcattctg cacctggcgg acggcccgag
     6001 ccacgggggc ggggacgacg aggcgccggt cgcgcaagtc aaggccgtgc aggtcctttt
     6061 gcatggcttc cttggtcgcc gccttggggc cgacatcgag ttgctcataa tgtttgccgt
     6121 agcgttgcag cgcgatggaa tcagccgtca ggagtccgag ccgcacgaga tacttcgccg
     6181 tccagtcctc gccgaagtac gaacccatgc cgtagatgct cccgtagtcg ccgaggttcg
     6241 cctgctgaaa ggcggccttg ccggcgacga tgtccccgcc cgtgaacaat acgtcgccgt
     6301 tcggcgcgac gatctgttcc ggctgcggcg gagcatgctg ataggacacg taggtgccgt
     6361 aggcgagggc gccgagtgtg acgatgccga cgaaaaacag ggtccacttc agataaaagc
     6421 tggtgcggtc gtggtccgat gcgatcagtt cttcggcgat ctcgctcatc ggggtgtcct
     6481 tccagtctga ctggcggtga agcagcaccg ggctgttact tgccgtggca ggtagcgctg
     6541 cgggcgcacc gcaagttcgc tctcgacccg cgccgcatca tcgccggggc catcgtccgg
     6601 gtgcattgat ctgcgtcaag cagagagact cgcgcgattc gagtgagcgc tggtctgcac
     6661 cggacgcggt ccggcccgac gctttggggc gatcggcgtc gggtttcgca tgcctgccgc
     6721 agccgcgtcg cccttcgatc gcggatgcgg cttc
//
LOCUS       DASUJG010000137         2333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1507216, whole genome shotgun sequence.
ACCESSION   DASUJG010000137 DASUJG010000000
VERSION     DASUJG010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507216"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VFX38_02825"
     CDS             complement(<1..870)
                     /locus_tag="VFX38_02825"
                     /inference="COORDINATES: protein motif:HMM:NF024215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family membrane protein"
                     /protein_id="HEX5313825.1"
                     /translation="MPAREFQVVKWRSLARRLPHGLAPRLRLRFAPNQIMLGFALRAI
                     LSIALPLTIFRLAGWAPAGLYVALGALQITLGGSGGAYRDRVLGIVLLCLLLPEIYLL
                     GTQVGTLWWIAAPVMFLLAFASGMLRVFGAAGAPLGLLVGATYLVGTLSATPAPAALL
                     HTGLFLAGCLWTLALTLAAWWLRPYVALRRDVATAIDAAATLLAALPERDADELTQEA
                     LEVRATIETARGALGVTHDMSAATNDALAALYALLHLPSRMATLALDLAQLRPRLAAE
                     ASALASFDAGCIAL"
     gene            954..1415
                     /locus_tag="VFX38_02830"
     CDS             954..1415
                     /locus_tag="VFX38_02830"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5313826.1"
                     /translation="MAGYTHVLAAVDLGADTELLLSRASEVAQRFGARLSLAHVVEYV
                     LAEPAGETLLPPPMTLEPELASGAERQLAEFAARAGAGEADRHVAVGSIPAELSRLVE
                     DLGADLLIAGAHERHWLAFFASGTERSLLKRVACDVLVVRLPKPRDGDASS"
     gene            1580..1960
                     /gene="apaG"
                     /locus_tag="VFX38_02835"
     CDS             1580..1960
                     /gene="apaG"
                     /locus_tag="VFX38_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEX5313827.1"
                     /translation="MGSLRREITVNVESSFLAGESEPEDSRYVFGYTVTLANTGDIGA
                     RLLTRHWIITDADGEVREVHGEGVVGEAPYLTPGAGFRYSSGAVLATPVGTMHGSYQW
                     IGDDGVPFDAPIPMFRLAVPGLLH"
     gene            1979..>2333
                     /locus_tag="VFX38_02840"
     CDS             1979..>2333
                     /locus_tag="VFX38_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002811020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5313828.1"
                     /translation="MAVFAIGDVQGCAQALKSLLARIRFDPADDLLWFTGDLVNRGPR
                     SLEVLRFVRGLGERAITVLGNHDLHLIAMAAGLKPPTPGLAAVLEAEDGAELIAWLRA
                     RPLLHHDPRYGYVLVH"
BASE COUNT          384 a          785 c          809 g          355 t
ORIGIN      
        1 gagagcgatg cagcctgcat cgaaggacgc gagcgcggaa gcttcggcgg cgagtcgcgg
       61 tcgcagttgc gcgagatcaa gcgcgagggt cgccatgcgc gagggcaggt gcagcagggc
      121 atacaaagcc gcgagcgcgt cgttggtcgc ggcgctcatg tcgtgcgtca cccccagcgc
      181 gccgcgcgcc gtttcaatcg tggcgcgaac ttcgagcgcc tcctgggtga gttcgtcggc
      241 gtcgcgttcg ggcagggccg cgagcagcgt tgccgccgcg tcgatcgcgg tcgcgacatc
      301 gcgccgcagc gcgacgtagg gccgaagcca ccaggcggcg agggtgagcg cgagggtcca
      361 caggcaaccg gcgaggaaca gccccgtatg caggagcgcg gctggagcgg gggtggcgga
      421 caaggttccg accagatagg tcgcgcccac gagcagcccc agtggcgcgc ctgcggcgcc
      481 gaacacccgc agcattccgg aagcgaacgc gagaaggaac atcacgggcg ccgcgatcca
      541 ccagagcgta ccgacctgtg tgccgagcag ataaatctcg ggcagcagca gacacaggag
      601 cacgatgccg aggacacgat cgcgataggc tcctccgctc cctcccagcg ttatctgcag
      661 cgcccccagc gccacgtaaa gtccggccgg cgcccatccc gcgagccgga atatcgtcag
      721 gggcagggcg atcgaaagga ttgcgcgcag cgcgaagccg agcatgatct ggtttggcgc
      781 gaatcgcagg cgaaggcgcg gggcgaggcc gtgcggaagt cgtcgcgcga gtgatcgcca
      841 cttcacgacc tggaactctc gcgccggcat gaggccattg acaaaacgct tccgcgcgcc
      901 gcaggatgca cacatcatag aggcgtgcac cgcctcgagc gaaggagttc gccatggccg
      961 gctatacgca cgtactcgcc gccgtcgacc tcggagccga tacggagttg cttctctccc
     1021 gcgcgagtga ggtcgcgcag cgcttcgggg cgcgcctgag cctcgcccac gtggtggaat
     1081 acgtcctcgc cgaaccggcc ggcgaaacac tgctgccgcc gccgatgacg ctcgagcccg
     1141 agctcgcgag cggcgcggaa cgccagcttg cggagttcgc cgcgcgcgcc ggcgccggcg
     1201 aggccgatcg tcacgtcgcg gtcggcagca tcccggcgga gctttcgcgt cttgtcgagg
     1261 atctcggcgc ggatctcctg atcgccggcg cgcacgaacg gcactggctc gcttttttcg
     1321 caagcggcac cgagcgttcg ctgctcaagc gggtcgcctg cgacgtgctg gtagtacgtc
     1381 tgcccaagcc ccgcgacggc gacgcgtcat cctgacaagg cttgcgggaa cttcgacatc
     1441 gaattgccac ggcgggtccg gcgaggttta cgcgcggctt gctgcgcgag ccccgcggag
     1501 gatcgcgggt tcgccgaacg gagcggccgc cgatccccgg cgctggtaga atcgcagcac
     1561 atccggcggg agaaagcagg tgggttcctt gcgacgtgaa atcacggtca atgtcgagag
     1621 cagtttcctc gccggcgaat cggaacccga ggacagccgc tacgtgttcg gctacaccgt
     1681 gactctcgcc aacaccggag acatcggcgc gcgcctgctc acccgccact ggatcatcac
     1741 cgatgccgac ggcgaagtgc gcgaggtgca cggcgagggt gtcgtcggcg aggcgcctta
     1801 tctcaccccg ggcgccggct ttcgctacag ctccggcgcc gtgctggcga cgcccgtcgg
     1861 aaccatgcac gggagctatc agtggatcgg cgacgacggg gttcccttcg acgcgccgat
     1921 ccccatgttc cgcttggccg tgcccggcct gttgcactga gtcgcatgcc gaaccggcat
     1981 ggccgttttt gccatcggtg atgtccaggg ttgcgcgcag gcgctgaaga gtcttctcgc
     2041 ccgcatccgc ttcgatccgg ccgacgatct tctgtggttc accggcgatc tcgtcaatcg
     2101 cggcccccgc tcgctcgagg tgctgcgctt cgtgcgcggg ctcggcgagc gcgcaattac
     2161 cgtgcttggc aatcacgatc tgcatctgat cgcgatggcg gcgggactga agccgccgac
     2221 tcccgggctc gccgccgtac tcgaagccga ggacggagcg gaactcatcg cctggctgcg
     2281 ggcgcgcccg ctgttgcacc acgatccacg gtacggctac gtgctcgtgc atg
//
LOCUS       DASUJG010000138         2825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_39513, whole genome shotgun sequence.
ACCESSION   DASUJG010000138 DASUJG010000000
VERSION     DASUJG010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39513"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="VFX38_02845"
     CDS             complement(<1..848)
                     /locus_tag="VFX38_02845"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5313829.1"
                     /translation="MRRGAWRFVVVIGVVSLFADMAYEGARGIVGAYFYQLGAGALLV
                     GVIGGGAEFLGYAVRWFAGRGADVSGRHWLFIYGGYALNMIAVPCLALTGTVGAAAAL
                     VAGERVGKGVRTPPRDALLARAGEVVGHGAGFGRHEFLDQLGAIAGPLIVAAVVVVAG
                     YRAGFAVLAIPAAAALAALAFARRFEIQPRQATATAGRLPRAFWSWVIFAALATAGFA
                     HFSLVAFDLAKIGFMPALIPVLFAVAMAAEGVAGWVFGHLSDRTGAMLLAGFPLCGLA
                     GTVLLFL"
     gene            complement(990..1382)
                     /locus_tag="VFX38_02850"
     CDS             complement(990..1382)
                     /locus_tag="VFX38_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3224 domain-containing protein"
                     /protein_id="HEX5313830.1"
                     /translation="MNHHATGTFDVKPAPQAAEGEAEAGPISRTKLVKQFHGDLEASG
                     TVQMLGVMTATRGSAGYVAMETVTGTLDGRKGGFTLQHSGTMTRGALRLSVTVVPDSG
                     TGELAEIAGEMTIDIVDGEHRYEFEYKL"
     gene            complement(1379..2005)
                     /locus_tag="VFX38_02855"
     CDS             complement(1379..2005)
                     /locus_tag="VFX38_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX5313831.1"
                     /translation="MIGAAEAAPVTYQIDPTHTYPSFAADHMGLSTWRGKFDHTTGTI
                     MLDRAAGTGSVHIVTEVSSINFGYPPLKKMVLGAAMPPALCKEQCAFFDAERFPTAVY
                     DGRLADFVHGAPTRVIGKLTLHGVTRPLILDINSFHCVPDPVLKPRERCGADASGTFD
                     RAAFGIDAGESYGFDMKVTLAIQVEAVQSETAAPSGSSSQTQNSGRRP"
     gene            complement(2193..2600)
                     /locus_tag="VFX38_02860"
     CDS             complement(2193..2600)
                     /locus_tag="VFX38_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012166946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5313832.1"
                     /translation="MKLNSYLHFDGDCKQAFEFYAETLGGKLEGVHLWADMPGEDVPP
                     ERRDKIMHASLRVGEELLMGSDSPPEYYEEPKGFSVTLNIDDATEAERIFKALARDGK
                     VSMPFAKTFFAEGFGMVTDRFGIPWMVNCEKPA"
     gene            complement(2678..>2825)
                     /locus_tag="VFX38_02865"
     CDS             complement(2678..>2825)
                     /locus_tag="VFX38_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX5313833.1"
                     /translation="DWTHPIGKEFVNVHAYRRRLLARPSFAKAVDEARPYRPLFPLGA
                     PERN"
BASE COUNT          537 a          938 c          847 g          503 t
ORIGIN      
        1 agaaacagga gcacggtgcc ggcaagtccg cacagcggga acccggcgag caacatcgcg
       61 ccggtacggt cgctcaagtg cccgaacacc catcccgcca cgccctcggc cgccattgcc
      121 acggcaaaca gaaccggtat cagcgccggc atgaacccga tcttcgccag atcgaaggca
      181 accaaagaga aatgtgcgaa gccggcggtg gcgagcgccg cgaaaatgac ccacgaccag
      241 aacgcccgcg gcaatctgcc ggccgttgcc gtggcttgcc tcggttggat ctcgaagcgc
      301 cgggcaaagg ccagtgcggc cagcgcagca gcggcgggaa tcgcaagaac ggcgaagccg
      361 gcgcgataac ccgcgacgac gaccaccgcg gcgacgatca gaggcccggc gatcgctccc
      421 agttgatcga gaaactcatg ccgtccgaag cccgcgccgt ggccaaccac ctccccggct
      481 cgcgccagca aggcatcgcg cggcggcgtg cgcactccct tgccgacgcg ctcgcccgca
      541 accagcgcgg cagccgcacc gaccgtgccg gtcagcgcga ggcagggcac cgcgatcatg
      601 ttgagcgcat accctccgta gatgaaaagc caatgacgcc cgctcacatc cgctccacgt
      661 cccgcgaacc agcgcacggc gtagccgaga aattcggcgc cgccgccgat cacgccgacc
      721 agcaacgccc ccgcgccgag ttgatagaaa tatgcgccga cgatgccgcg cgcgccctcg
      781 taggccatgt cggcgaagag actcaccacg ccgatcacga cgacgaagcg ccacgctccg
      841 cgccgcatcg ttcgacccgc ttcgctcact tcggagttcg gctgcgattc ggaagcttta
      901 ttctagagtt tcgcgatgtc gccggccaga aaaccgcttg atctcgcccg ggcccccgcc
      961 tcgtgaatcc gctttcgcgg gcggacaagc tacagcttgt actcgaattc gtagcgatgc
     1021 tccccgtcaa cgatatcgat tgtcatctcg ccggcaattt ccgcgagttc gccggtaccg
     1081 gaatcgggga ccacggtgac gctgagccgg agtgcgcccc gcgtcatggt gccgctgtgt
     1141 tgcagcgtga aaccgccttt gcgcccgtcg agcgtgccgg tgacggtttc catcgccacg
     1201 tagcccgccg atcccctggt cgccgtcatc acgccgagca tctgcaccgt gccggatgct
     1261 tcgagatccc cgtggaattg cttgacgagc ttcgttctgc ttatcggccc ggcctcggcc
     1321 tcgccttccg ccgcctgcgg cgccggcttg acatcgaagg ttcccgtcgc gtgatgattc
     1381 atggtcgccg tcccgagttc tgcgtttgag aagaggaacc ggacggcgcg gcggtttcgg
     1441 actggaccgc ctccacctga atcgcgaggg tgaccttcat gtcgaacccg tacgactcgc
     1501 cggcgtcgat gccgaaggcg gcgcgatcga aggttcccga agcatccgcg ccgcagcgtt
     1561 cccgcggctt caggaccgga tcgggaacgc aatggaagga attgatgtcg agaatcaagg
     1621 gacgcgtgac gccatgcagg gtcagcttcc cgatcacgcg cgtcggcgca ccgtgaacaa
     1681 agtcggcaag cctgccgtca tacacggcag tcggaaatct ttcggcatcg aagaacgcac
     1741 attgttcttt gcacagcgcc ggcggcattg ccgcgcccag taccatcttc ttcagcggcg
     1801 ggtacccgaa attgatgctc gacacttcgg tcacgatgtg cacgctgccg gtgccggccg
     1861 cgcggtccag catgatcgtg ccggtggtgt gatcgaactt gccgcgccac gtcgaaaggc
     1921 ccatgtggtc ggcagcgaag ctcggatacg tgtgcgtcgg atcaatctgg taggtgaccg
     1981 gagcggcttc agccgctccg atcatcgtca ctgacgacaa gctgataacg attgctgcgt
     2041 acaacgcgaa tttgaatgac acgctccttc tcctgtgttc agatccatag actcgcatac
     2101 gccgcaaagc agtgtggcgg gtatcctgcc cggacgccgg caacggcctt ccggccttat
     2161 gccggcctac atttccgaca tttacgttcc ggtcacgcgg gcttttcgca gttcaccatc
     2221 cagggaatgc cgaagcgatc cgtcaccatg ccgaaacctt cggcaaagaa ggttttcgcg
     2281 aacggcatgc ttacctttcc gtcgcgggca agcgccttga agatacgttc cgcttccgtc
     2341 gcatcgtcga tgttgagcgt caccgaaaaa cccttgggct cctcgtagta ctccggcggc
     2401 gaatcgctgc ccatgagcag ctcctcgccg actcgcaggc tcgcgtgcat gatcttgtcg
     2461 cgacgttcgg gcggcacgtc ctcgcccggc atgtcggccc agaggtgtac gccttcgagc
     2521 ttgccgccga gagtctccgc atagaactcg aaggcctgtt tgcagtcgcc gtcgaaatgg
     2581 aggtaggaat tcagtttcat gggggtactc ctttacgtga ttgcagagta gaataaccgc
     2641 gataagtgtc gtctaactca ctttatttac atacgtttca attgcgctcg ggtgcgccga
     2701 gcggaaagag cggacgatac ggccgtgcct cgtccaccgc cttggcgaac gagggccgtg
     2761 caagcaggcg ccgccgatac gcatgcacgt tgacgaattc cttgccgatg gggtgagtcc
     2821 agtcg
//
LOCUS       DASUJG010000139         6005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_284967, whole genome shotgun sequence.
ACCESSION   DASUJG010000139 DASUJG010000000
VERSION     DASUJG010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6005
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284967"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..638
                     /gene="sdhC"
                     /locus_tag="VFX38_02870"
     CDS             216..638
                     /gene="sdhC"
                     /locus_tag="VFX38_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEX5313834.1"
                     /translation="MISETRPTSPHIGIYRWRITMTLSILHRASGIVLYSGAIGLVYW
                     LVSLALGPRIFAMASWFFHFWVGQALLCLWSLAFFYHLLNGVRHLVWDTGRGFDKPIH
                     RERGALVYRVSGWVVVVSALILTAAAWAFGWLLSAGVL"
     gene            635..1015
                     /gene="sdhD"
                     /locus_tag="VFX38_02875"
     CDS             635..1015
                     /gene="sdhD"
                     /locus_tag="VFX38_02875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEX5313835.1"
                     /translation="MKFRSPLATARGRGSAHNGTEHWFAQRLGALALIPLGLAAAVLF
                     FWLMRSGYYPVFALMHRPWVLLFAVLLVAVAFWHGYLGLRVVIEDYFAPAPAFVLIAL
                     VRFLSVALALLGIIAAAMVGLRSF"
     gene            1015..2802
                     /gene="sdhA"
                     /locus_tag="VFX38_02880"
     CDS             1015..2802
                     /gene="sdhA"
                     /locus_tag="VFX38_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEX5313836.1"
                     /translation="MTGGDYDIVEHRYDVVVVGAGGAGLRATLGLAEAGLKTACVTKV
                     FPTRSHTVAAQGGMAASLGNMGEDHWSWHMYDTVKGSDWLGDQDAIEYLCREAPASVL
                     ELEHYGLPFSRTETGNIYQRPFGGMTTRFGEGTAHRTCAAADRTGHAMLHTLYQQSLK
                     YRAEFFIECFALDLLMEDGVCRGLLAWDLMTGKLHRFAAQQVILATGGYGRVYFSCTS
                     AHICTGDGGGMVLRAGLPLQDMEFVQFHPTGIYGAGCLITEGSRGEGGYLTNAEGERF
                     MERYAPHAKDLASRDVVSRAMTIEIREGRGIGTDKDHLELHLEHLGAEVLAKRLPGIS
                     ETAHIFAGVDVNREPIPVLPTVHYCMGGVPTNLHGEALTRSGDDPDSVIPGLMAVGEA
                     ACVSVHGANRLGSNSLIDLVVFGRAAAKRCAETVKPGHTAELRAQTGEEALARFDRFR
                     HAAGAAPTAKIRDRMQRTMQRDAGVFRDKDTLAEGKEKIAEVAAMLQDVGVSDRSLVW
                     NSDLAETLELANMLPQAVVTMQCASNRTESRGAHAREDYPDRDDGQWMKHTLAWCDET
                     NRVRIDYRPVHDYTLSGEIEPFPPKKRVY"
     gene            complement(2783..3022)
                     /locus_tag="VFX38_02885"
     CDS             complement(2783..3022)
                     /locus_tag="VFX38_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313837.1"
                     /translation="MKVLPFPMREFFGNLICAIVSSPSASQVGTSPQALRHPAGRRER
                     FADPGLQTQGIANKLAPATVSLFTSLTHFVNIPAS"
     gene            2967..3755
                     /locus_tag="VFX38_02890"
     CDS             2967..3755
                     /locus_tag="VFX38_02890"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEX5313838.1"
                     /translation="MAQIRLPKNSRIGKGKTFKAEGAKNVKRYQIYRYDPDAGENPRM
                     DSYEVDRDNCGPMVLDALIKIKNEIDPTLTFRRSCREGVCGSCAMNIGGKTGGMNTLA
                     CTKAQDDIAGDVRIYPLPSMPVVKDLVPDLTHFYAQYAAIQPWLKADSAEPSRERLQS
                     PEQRERLDGLSECILCACCSAACPSYWWNPDRYLGPAILLQAQRWLADSRDEATGERL
                     DFLQDSFRLYRCHTIMNCTDTCPKHLNPGKAIADIKKAIAEREL"
     gene            3767..4033
                     /locus_tag="VFX38_02895"
     CDS             3767..4033
                     /locus_tag="VFX38_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEX5313839.1"
                     /translation="MNRDLCSRADDEESPARLRWRSRRGLRELDLMLERYLESGYPQA
                     DAEERQAFARLLECADPDLVAWLLGQTPAPAGLEHVVRAIAAAR"
     gene            4002..4514
                     /locus_tag="VFX38_02900"
     CDS             4002..4514
                     /locus_tag="VFX38_02900"
                     /inference="COORDINATES: protein motif:HMM:NF018908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HEX5313840.1"
                     /translation="MSFERSPPLVDLPLGASRSLAALCALAHALAIAASWASGLPRIA
                     AAALTIALAAHGLWWIASQGLRILPSSLRRFVWQPDGECILVQRRSRNWSGRVLPGHL
                     ASSALTVIRVGAGRERRVIVVARDAVDAEAFRRLRVRLNLAPPPVRPPLLRRCIGRAR
                     SLLREGPSEA"
     gene            4852..5430
                     /locus_tag="VFX38_02905"
     CDS             4852..5430
                     /locus_tag="VFX38_02905"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEX5313841.1"
                     /translation="MKHLHVIRWAALGGLLLTPLAALAVGGPMSGWTGAYVGAQAGLN
                     NVSSDQTSSENAVTLGLLGGYDAQVSDHFVLGGDVFYNWNRKKDHSIDNCIGPCTTNY
                     GTNVYGVDLRLGFPIGTSGGIMPYVKGGYGRANGTGDLSGDSNAWRYGAGIEFRPGDF
                     LGLTLQYMHQNFGSDSDDLTNDNYTVGVNFHF"
     gene            complement(5547..>6005)
                     /gene="acnA"
                     /locus_tag="VFX38_02910"
     CDS             complement(5547..>6005)
                     /gene="acnA"
                     /locus_tag="VFX38_02910"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952423.1"
                     /note="catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEX5313842.1"
                     /translation="TIHVPDGETMSIYDASMKYQQDGTPLIVIAGSEYGSGSSRDWAA
                     KGTLLLGVKAVIAKSFERIHRSNLIGMGVLPLEFLPGEDAESLGLDGREEYAISGLAD
                     GNAREASVTARTDDGREIRFRARVRLDTPKEREYYANDGILQYVLRQLAA"
BASE COUNT          988 a         1890 c         1966 g         1161 t
ORIGIN      
        1 ccggacccga gccgacgcgc tatggcgact gggaacgcaa ggggcgctgc attgatttct
       61 gacgtctcgc cgcaggacgt ctcccttgat gccccgttct cgcggaggag ggagatgtgc
      121 ctcttcgctc ccttcgtcgt acgaaggggg ccgcgtcttc gcggggggat gtcttccttg
      181 caccacgcag aaacgtcgcg agcgggaacg caagcgtgat ttccgaaacc cgtccgacat
      241 cgccgcacat cggcatttac cgctggcgga tcacgatgac gctctcgatt ctgcatcggg
      301 catcgggcat cgtgctctac tcgggcgcca tcgggcttgt gtactggctg gtgtccctgg
      361 cgctcggccc gcgcatattc gcgatggcga gctggttctt tcatttctgg gtgggacaag
      421 cgctcctctg tctgtggagt ctcgcgtttt tctatcatct cctgaacggc gtgcgccatc
      481 tcgtctggga taccggccgc ggattcgaca agccgattca tcgcgagcgc ggtgcactgg
      541 tttatcgcgt gagcggatgg gtcgtggtgg tgagtgcgct gattcttacc gccgcggcct
      601 gggcgttcgg ctggctgctt tcggcggggg tgctgtgaag ttccgctcgc cgctcgcaac
      661 ggcgcgcggg cgcggctcgg cgcacaacgg cacggagcac tggttcgccc agcgcctcgg
      721 tgcgctcgcc ctgattcctc tcggactcgc ggccgcggtg ctcttcttct ggctgatgcg
      781 cagcggctac tatccggtct tcgcgctgat gcaccgtccc tgggtgctgc tgttcgccgt
      841 gcttctggtc gccgttgcat tttggcacgg ttatctcggg ctccgggtgg tcatcgagga
      901 ttatttcgcg cccgctcccg cgttcgtcct gattgcgctc gtgcgatttc tgagcgtcgc
      961 gctcgcgctc ctcggcatca tcgctgccgc gatggtgggt ctgcggagtt tctgatgacc
     1021 ggcggcgatt acgacatcgt cgaacatcgc tacgatgtcg tcgtggtcgg tgcgggcggc
     1081 gcgggcctgc gtgcgaccct cggactcgcc gaagccgggc tcaagaccgc ctgcgtgacc
     1141 aaggtgtttc ccacccgcag tcacaccgtc gccgcccagg gcgggatggc cgcctcgctc
     1201 ggcaacatgg gcgaggatca ctggtcctgg cacatgtacg acaccgtcaa gggttccgac
     1261 tggctgggcg atcaggacgc gatcgaatac ctctgccgcg aggctccggc ctcggtgctc
     1321 gaactcgaac actacgggct tccgttttcg cgcaccgaga cgggcaatat ctaccagcgg
     1381 cccttcggcg gcatgaccac gcgcttcggc gagggcacgg cgcatcgtac ctgcgccgcg
     1441 gccgatcgta ccggtcacgc gatgctgcat acgctgtacc agcagtcgct caagtatcgc
     1501 gccgagtttt ttatcgagtg cttcgcgctt gatctcctga tggaagacgg agtgtgccgc
     1561 gggcttctcg cctgggatct catgaccggc aagctgcacc gcttcgccgc gcaacaggtc
     1621 attctcgcga ccggcggcta cgggcgcgtt tatttctcct gcacttcggc gcacatctgc
     1681 accggcgatg gcggcggcat ggtgctgcgc gcggggctgc ccctgcagga catggaattc
     1741 gtgcagtttc atccgaccgg aatctacggc gcgggctgtc tgatcacgga gggttcccgc
     1801 ggcgagggcg gttatctcac caacgccgag ggcgagcgct tcatggagcg ttatgcgccg
     1861 cacgcgaagg atctcgcctc gcgcgatgtc gtcagtcggg cgatgaccat cgagatccgc
     1921 gagggccgcg gcatcggcac ggacaaggat catctggaat tgcatctcga gcatctcggt
     1981 gccgaggtgc tcgccaagcg tctgccggga atctccgaga ccgcgcacat cttcgccggc
     2041 gtggacgtga accgggagcc cattcccgtg ctccctaccg tccactactg catgggcggc
     2101 gtgccgacca accttcatgg cgaggcgctt acgcgctcgg gcgacgatcc cgacagcgtg
     2161 attccgggat taatggcggt cggcgaagcc gcctgcgtgt ccgttcacgg cgcgaaccgg
     2221 ctgggctcga attccctgat cgatctcgtc gtgttcggtc gcgcggcggc gaagcgctgc
     2281 gccgagacgg tgaagccggg gcataccgcc gagttgcgcg cacagacggg cgaggaggca
     2341 ctggcgcgct tcgatcgttt ccgtcacgcc gcgggtgctg cgccgaccgc gaagattcgt
     2401 gatcgcatgc agcgcaccat gcagcgggat gccggcgtct ttcgcgacaa ggacacgctc
     2461 gccgaaggga aggagaaaat cgccgaggtt gccgcgatgc tgcaagacgt cggagtcagc
     2521 gaccgttcgc tcgtctggaa ttcggacctc gccgaaacgc tcgagcttgc gaacatgctg
     2581 ccgcaggccg tggtcacgat gcaatgtgcc tccaatcgca ccgaaagccg gggagcgcat
     2641 gcacgtgagg actatccgga tcgcgacgac gggcagtgga tgaagcatac gctcgcctgg
     2701 tgcgacgaga cgaacagggt gcgcatcgac tatcggccgg tgcacgacta cacgctcagc
     2761 ggcgaaatcg agccgtttcc gcctaagaag cgggtatatt gacgaagtga gtgagagaag
     2821 tgaaaagcga gacggtggca ggagcgagct tgttcgcgat tccttgagtt tgtaggccgg
     2881 gatcggcaaa gcgttcccgg cgaccggccg gatgccgcaa cgcctgcggg cttgtgccga
     2941 cctgcgaagc ggaaggagat gagacgatgg cgcaaatcag attgccgaag aattcgcgca
     3001 tcgggaaggg caagaccttc aaggcagaag gcgcgaagaa cgtgaagcgg tatcagatct
     3061 accgctacga cccggatgcg ggcgagaatc cccgcatgga cagctacgag gtggaccgcg
     3121 acaactgcgg cccgatggtg ctcgatgcgc tgatcaagat caagaacgaa atcgatccca
     3181 cgctcacctt ccggcgcagt tgccgcgagg gggtgtgcgg ttcctgtgcg atgaacatcg
     3241 gcggcaaaac gggcggcatg aacacgcttg cctgcacgaa ggcgcaggac gacattgccg
     3301 gagacgttcg catttatccg cttccgtcga tgccggtggt gaaggatctg gttcccgatc
     3361 tcacgcactt ctacgcgcaa tacgcggcga ttcagccctg gctcaaggcc gactcggccg
     3421 aaccgagtcg cgaacgcctg caatcgcccg agcagcgcga gcgcctcgac ggcctgtccg
     3481 aatgcattct ctgcgcctgc tgctccgccg cctgcccgag ctactggtgg aatccggatc
     3541 gctatctcgg tccggcgatt ctcttgcagg cgcagcgctg gctcgccgac agtcgcgacg
     3601 aggcgacggg agaacgtctc gatttcctcc aggattcctt ccgcctgtac cgctgccaca
     3661 cgatcatgaa ttgcaccgac acttgcccga agcatctcaa tcccggcaag gcgattgccg
     3721 atatcaagaa ggccattgcg gagcgggagc tgtagccgcg tcggccatga accgggatct
     3781 gtgctcgcgg gcggatgacg aggaatcgcc ggcgcgcctg cgctggcgtt cgcgaagggg
     3841 gctgcgcgaa ctcgatctga tgctggaacg ttacctcgaa tcgggctatc cccaggccga
     3901 tgccgaggaa cggcaagcct tcgcgcgcct gctcgaatgc gccgatcccg acctcgtcgc
     3961 ctggctgctc gggcaaacac ccgcaccggc ggggctggaa catgtcgttc gagcgatcgc
     4021 cgccgctcgt tgatcttccg ctcggagctt cgcgctcgct tgcggcgctg tgtgcattgg
     4081 cgcacgcgct cgccatcgcg gcgtcgtggg caagcggact gccgcggatt gcggcggccg
     4141 cgctgacgat cgcgctcgcc gcgcatgggc tgtggtggat cgcgtcgcag gggttgcgta
     4201 ttcttccctc gtcgctgcgg cggttcgtct ggcagcccga cggcgaatgt attctggtgc
     4261 aacgccggag tcgaaactgg agcggcaggg tcttgcccgg acacctggcg agttcggcgc
     4321 tgaccgtgat acgcgtcggc gccgggcgcg aacgacgggt gattgtcgtg gcgcgcgatg
     4381 ccgttgacgc cgaggcgttt cgccgcctgc gggtacggct gaatctcgcg ccgcctcccg
     4441 tacgcccgcc gcttctgcgc cgatgcatcg gccgcgcccg ctcgctgctg cgcgaaggac
     4501 cttccgaagc ttgaactaat ccccgcatcg cgggtctcaa ccacgcagac atcggcacgc
     4561 aaggatgcgt atgcggagtt tgccggcctt cggccgcggt gaatgcccga gagtcgttcg
     4621 ggttttcacc cagtgcaggg atgcatcggc gcaatcgggc tgccatgccc gtttggggca
     4681 agcccggcaa tgttgccggg cttgccggcc tcttgcggat cgcatcctgt tgatgctcga
     4741 atggatggac cctttcggaa ctctcgtcgg aagcaaaatc gcgacgcgca tcccggattt
     4801 tcccggatcg gctccgctcg gagccttctt tactcaacaa ggaggatggt aatgaaacat
     4861 ctgcacgtca ttcgatgggc cgcgctgggc ggtctcctgc tcactccgct tgccgctctc
     4921 gcggtcggcg gtcccatgtc cgggtggacc ggcgcgtacg tcggggccca agccggattg
     4981 aacaacgttt cgagcgacca gaccagctcc gagaacgcgg tcaccctggg cctgctcggc
     5041 ggctatgatg cccaggtttc ggatcatttc gtcctcggcg gtgatgtctt ctacaactgg
     5101 aaccgcaaga aggatcattc gatcgacaat tgcattgggc cctgcaccac caattacggc
     5161 acgaacgtgt atggcgtgga tctgcgtctc gggtttccga tcggcacgtc cggcggcatc
     5221 atgccgtatg tgaaaggtgg ttacggccgg gcgaatggca ccggcgatct cagcggcgac
     5281 agcaacgcct ggcgttacgg tgccggcatc gagtttcgtc caggcgactt tctgggtctg
     5341 acgctccagt acatgcacca gaatttcgga agcgacagcg acgatctcac caacgacaac
     5401 tacacggtcg gcgtcaactt tcacttctga ccgattgtcc cctggacagg agcttcgtgc
     5461 gggtcgggga agacccgcgc gaagccggtg gcagccgcga aaactcttcg ttctctttgc
     5521 gaccgattcc gtagccgtgc aagcgctcag gcggcgagct ggcgcaacac gtactgcaga
     5581 attccgtcgt tcgcgtaata ttcccgttcc ttgggggtgt cgagacgcac gcgggcgcgg
     5641 aaccgaatct cccggccgtc gtctgtccgg gcggtgacgc tcgcttctct ggcgttgccg
     5701 tccgcaagcc cggagatcgc gtattcctcg cggccgtcga gcccgagcga ttcggcatct
     5761 tcgccaggca aaaattcgag cggaagcacg cccatgccga tgagattcga gcgatgaatg
     5821 cgctcgaagc tcttcgcgat gacagccttc acgccgagca gcagcgtgcc cttggcggcc
     5881 cagtcgcgcg aggaaccgct gccgtattcg ctgccggcga tgacgatgag cggcgttccg
     5941 tcctgctggt acttcatcga agcatcgtag atgctcatcg tctcgccgtc gggaacgtgg
     6001 atggt
//
LOCUS       DASUJG010000140         5742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1385918, whole genome shotgun sequence.
ACCESSION   DASUJG010000140 DASUJG010000000
VERSION     DASUJG010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5742
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1385918"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VFX38_02915"
     CDS             complement(<1..270)
                     /locus_tag="VFX38_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313843.1"
                     /translation="MDASAGFAAPRLLPEGSDSAHDVRAPCADPGAIFQQEGARLLKR
                     SQYRLADSSPSARTIEAPKRAPMAHGRGSGRLDGAATRLRKYTGAA"
     gene            269..1657
                     /locus_tag="VFX38_02920"
     CDS             269..1657
                     /locus_tag="VFX38_02920"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HEX5313844.1"
                     /translation="MQQLRRRDGALVVLLRVADGAVCIAAGLGAFVLRFGMRGVPDAY
                     QVALAVATLLVLLLFPLLRVYETYRMRSVAAPSVRVIVAWAIVIGVLVVLTVFAGRGT
                     RFSRLWLGEWFGLGVVAYVLLRVGCYLALRKLRRLGRNLKRVALVGDGPLAEALLERI
                     TTERWMGFRLVAHYHAGADVASCHPAVAAGGRTLKVLLDAREVDEIWIAATPDQGATV
                     RALCALSRSYTTTVRYVPDFRDLFLLNHGITEIAGLAMIDLTASPLQGVNGFLKRTED
                     LLIASCALILASPVMFAIALAVRLTSPGPVIFRQLRHGWGGEEIEVYKFRTMRVHREE
                     GGVTQANRNDTRITPLGRLLRRTSLDELPQLINVLQGRMSLVGPRPHAREHNELYQAQ
                     IEGYMQRHKMRPGITGWAQVNGLRGETDTLDKMKHRVEFDLFYIGHWSLWLDLKILVL
                     TVLRGFVNPNAY"
     gene            1718..3439
                     /locus_tag="VFX38_02925"
     CDS             1718..3439
                     /locus_tag="VFX38_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008557606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional sulfate
                     adenylyltransferase/adenylylsulfate kinase"
                     /protein_id="HEX5313845.1"
                     /translation="MNRTSEQKAAPERVRELYVPRENCEARKAESAALREWTLTHRQI
                     CDLELLMSGGFAPLDGFLGRGDYDGVVAEMRLASGALWPMPITLDVTREFADSLAPGE
                     QIALRDPEGVALAILTAGDVWEPNKEQEAEGVFGAADPAHPAVRYLLDEAGPVYVGGK
                     IEGLSTPSHYDFKHLRLTPNELRERFARLGWRRIVAFQTRNPMHRAHMELTFRAAREA
                     EANLLIQPSVGMTKPGDIDHFTRVRCYEHLVQHYPEQTTMLSLLPLAMRMGGPREALW
                     HALIRRNHGATHFIVGRDHAGPGKRSDGTPFYGPYDAQKLVGEHAAETGITMLPFRQM
                     VYVRERAQYAPEDEIAEGETVLNISGTEFRRRLAEGLDIPEWFSFPEVVKELRRTHPP
                     LDRRGFTVFFTGLSGSGKSTIANALMVKLLELGGRRVSMLDGDLVRRNLSSELGFSKE
                     HRDLNIRRIGYVASEITNHGGIAICAPIAPYAATRRAVREMVEATGGGFIEAFVSTPI
                     ETCEERDRKGLYARARAGKIKEFTGISDPYEAPEKPEITIDTRGNTPDEAAQDIMLKL
                     ERLGYIR"
     gene            3436..4422
                     /gene="galE"
                     /locus_tag="VFX38_02930"
     CDS             3436..4422
                     /gene="galE"
                     /locus_tag="VFX38_02930"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011627914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEX5313846.1"
                     /translation="MKLLVTGGAGYIGSHVTRQLAQAGHDVVVFDNLSTGQAHAVLGA
                     QLVVGDLADGAILDALFRRHDFEAVLHFAASIVVPESVARPLAYYANNTRNMLNLLER
                     VAGFEVPGFVFSSTAAVYGMPDVDAVGEDLAPAPINPYGASKAMSERMLADLARAKPA
                     LRYVALRYFNVAGADPQCRIGQETPEATHLIKVACQAALGRRDGVDLFGTDYPTPDGT
                     CIRDYIHVEDLARAHLDALRYLADGGASAVLNCGYGRGASVREVLAIAREVTGVDFPL
                     REAARRPGDPARLVAAVERIGAALHWKPRYADLPTIIAHAWAWERHLAARKH"
     gene            4497..4910
                     /locus_tag="VFX38_02935"
     CDS             4497..4910
                     /locus_tag="VFX38_02935"
                     /inference="COORDINATES: protein motif:HMM:NF037928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HEX5313847.1"
                     /translation="MAKIWVVVGDNSRARMFATNRAAPLEEREDYVPAYDPGQDKERF
                     TDRTAHQGHVAEGGRDPAAEADRRFVRRIAGRLREARLAGEYERLFLVAPPVFLGELR
                     QALDSGTREVVVGEAAKNLSQLRADDIRAHLPELL"
     gene            5021..>5742
                     /locus_tag="VFX38_02940"
     CDS             5021..>5742
                     /locus_tag="VFX38_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5313848.1"
                     /translation="MTKRVYIQTWGCQMNEYDSAKMRDVLAVEADYEPAASPGEADLI
                     LLNTCSVREKAQEKVFSQLGQWRALKEARPEILIGVGGCVASQEGEALLRRAPFVDLV
                     FGPQTLHRLPGLLAERRASGRAVVDISFPEIEKFDHLPAPRAEGPCAFVSIMEGCSKY
                     CSFCVVPYTRGEEISRDFDGVLAEVWELAERGVREITFLGQNVNAYRGRRHDGATADL
                     ASLIHYAAAIEGIERIRFTTSHP"
BASE COUNT          975 a         1913 c         1874 g          980 t
ORIGIN      
        1 cgccgcgccg gtgtatttgc gcagccgcgt agccgcgccg tcgaggcgcc cggagcctcg
       61 cccatgagcc atcggcgcac gtttcggcgc ctcgatggtc cgggccgaag gcgacgaatc
      121 ggcgagccgg tactgactcc gcttgagaag ccgggcacct tcctgctgaa atatagcccc
      181 gggatcggca caaggcgcgc ggacatcgtg agcggaatcg gagccttcgg gcaacaatcg
      241 cggggcggcg aagcccgcgc tggcgtccat gcagcagctt aggcgtcgcg acggcgccct
      301 ggtcgtgctt ctgcgcgtag ccgacggcgc cgtatgcatt gccgcgggtt tgggcgcctt
      361 cgtgctgcgc ttcgggatgc gcggagtgcc cgatgcctat caggtagcgc tggccgtggc
      421 gaccctgctg gtcctgctgc tctttccgct attgcgggtc tacgagacct atcgcatgcg
      481 ttccgtcgcc gccccgtcgg tccgggtcat cgtcgcctgg gccatcgtga tcggcgtgct
      541 ggtggtcctg accgtcttcg cggggagagg cacgcgcttt tcgcggctgt ggctggggga
      601 atggttcgga ctgggcgtcg tggcctacgt gctcctgcga gtcggctgtt atctcgccct
      661 gagaaagctc aggcggctcg ggcgcaacct gaagcgcgtc gcgctcgtcg gggacggacc
      721 tctcgcggag gccttgctcg aacgcatcac gaccgaaaga tggatgggat ttcgcctcgt
      781 cgcgcactat cacgccggag ccgacgtggc gagttgtcat cccgcagtcg ccgcgggcgg
      841 cagaaccctc aaggtgctgc tcgacgcgcg cgaggtggat gaaatctgga tcgccgccac
      901 gccggatcag ggggcgacgg tgcgcgcact ctgtgcgctg tcccgttcct acaccacgac
      961 cgttcgctac gttccggatt ttcgcgatct ctttctgctg aatcacggca tcaccgaaat
     1021 tgcgggcctc gcgatgatcg atctcaccgc gagcccgctg cagggagtga acggctttct
     1081 caagcggacc gaagacttgc tgatcgcctc gtgcgcgctg attctggctt cacccgtcat
     1141 gttcgcgatc gcgctcgcgg tccggctcac gtcacccggc cccgtcatct tccgccagct
     1201 tcgccacggc tggggaggcg aggaaatcga ggtctacaaa ttccgcacga tgcgcgtgca
     1261 ccgggaggag ggcggcgtta cccaggccaa ccgtaacgac acgcgcatca ctccgctcgg
     1321 gcgcctgctg cgacgcacga gtctcgacga acttccgcag ctcatcaatg tgcttcaggg
     1381 ccgcatgtcg ctcgtcggac cgcgccccca cgcacgcgag cacaacgaac tctatcaggc
     1441 ccagatcgag ggttacatgc agcgccacaa gatgcgcccc ggcataaccg gctgggcgca
     1501 ggtgaatgga ctgcgcggcg aaaccgatac cctcgacaag atgaagcatc gggtcgagtt
     1561 cgatctgttc tacatcgggc actggtcgtt gtggctggac ctgaagatcc tcgtgctcac
     1621 ggttctgcgc ggcttcgtga atccaaacgc ctactgagac atcggcgaag ccgcgcatgg
     1681 taaaatttcc ggttcttacg ggtcccggcg gcacgccatg aacagaacga gcgaacagaa
     1741 ggcggccccc gagcgggttc gcgaactcta cgtcccgcgc gagaactgcg aggcccgcaa
     1801 ggccgagagt gccgccttgc gcgagtggac gctcacgcat cgccagatct gcgacctgga
     1861 actcctgatg agcggcggct tcgccccgct cgatggcttt ctcggccgcg gcgattatga
     1921 cggcgtggtc gcggagatgc gactcgcctc cggcgcgctc tggccaatgc cgatcaccct
     1981 cgacgtgacg cgggaatttg ccgattcgct cgcaccgggc gagcaaatcg cgctgcgcga
     2041 tcccgaaggc gtcgcgcttg caatcctaac ggccggcgac gtctgggagc cgaacaagga
     2101 gcaggaggcc gaaggggtgt tcggagcggc cgatcccgcc catccggccg tacgctacct
     2161 gttggacgaa gccggaccgg tctatgtagg cggaaagatc gaaggtctct caacgccatc
     2221 ccactacgat ttcaaacatt tgcgccttac gcccaacgaa ctgcgcgaac gttttgcccg
     2281 cctcggctgg cgacgcatcg ttgcgtttca gacccgcaac cccatgcacc gcgcccacat
     2341 ggaactcacc tttcgcgccg cgcgggaggc cgaggcgaat ctcctgatcc agccgagcgt
     2401 cggcatgacc aagccgggag acatcgacca tttcacccgc gtgcgctgct acgaacatct
     2461 cgtccagcac tatcccgaac agacgacgat gctctcgctc ctgccgctcg cgatgcgcat
     2521 gggcggcccg cgcgaggcgc tctggcacgc gctgatccgt cgcaaccacg gggcgacgca
     2581 tttcatcgtc gggcgcgacc atgcgggtcc cggcaagcgc agcgacggca ctccttttta
     2641 cggtccctac gatgcgcaga aactcgtcgg cgagcatgcc gcggaaaccg gaatcacgat
     2701 gcttcccttc cgtcagatgg tgtacgtgcg cgagcgcgcg cagtacgcgc ccgaagacga
     2761 gatcgccgag ggcgagaccg tgctcaatat ttccggcacg gagttccgcc gccggctcgc
     2821 cgagggtctc gacattcccg agtggttttc ctttcccgaa gtcgtgaagg aactccggcg
     2881 tacgcatccg cctctcgatc ggcgcggatt cacggtgttt ttcaccggac tttccggctc
     2941 cggcaagtcc accatcgcca atgcgctgat ggtgaagctc ctcgaactcg gcgggcggcg
     3001 cgtcagcatg ctcgacggcg acctcgtgcg ccggaatctc tcgagcgagc tgggcttctc
     3061 gaaggaacat cgcgatctca acattcggcg catcggctac gtcgcgagcg aaatcaccaa
     3121 tcacggcggc atcgcgatct gcgcgccgat cgcgccgtat gcggcgacgc gccgcgcggt
     3181 gcgcgagatg gtggaggcga ccggcggggg attcatcgag gcctttgtct ccacgccgat
     3241 cgagacctgc gaggaacgtg accgcaaggg gctctatgcg cgcgcgcgtg ccggcaagat
     3301 caaggaattc accggcatca gcgatcccta cgaggcgccg gaaaagccgg aaatcacgat
     3361 cgacacgcgc ggcaatacgc cggacgaggc cgcgcaggac atcatgctca agctggagcg
     3421 cctcggctac atccgatgaa actgctggtg accggcgggg cgggatacat cggctcccac
     3481 gtgacccggc agctcgcgca agccggccac gacgtcgtcg tcttcgacaa cctttcgacc
     3541 ggccaggcgc atgccgtgct cggcgcgcaa ctggtggttg gcgatctcgc cgacggcgcg
     3601 attctggatg cgctgttccg gcgccatgac ttcgaggcgg ttcttcattt cgcggcgagc
     3661 atcgtggtgc ccgaatcggt tgcgcgaccg ctcgcctatt acgccaacaa cacccgcaac
     3721 atgctcaatc tactcgagcg cgttgcaggc ttcgaggttc cgggcttcgt cttttcttcc
     3781 accgccgcgg tctacggaat gcccgatgtg gatgccgtcg gcgaggatct cgcaccggct
     3841 cccatcaatc cctacggcgc ctccaaggcg atgagcgagc ggatgctcgc cgatctcgca
     3901 cgcgcaaaac cggcgctgcg ctacgtcgcg ctgcgttatt tcaacgtcgc cggtgccgac
     3961 ccgcaatgcc gtatcggcca ggagactccc gaggctacgc acctgatcaa ggtggcctgc
     4021 caggcagcgc tcggccggcg cgacggagtc gaccttttcg gcaccgacta tccgacgccc
     4081 gacggcacct gcatccgcga ctacatccac gtcgaggatc tcgcgcgcgc ccatctcgat
     4141 gcgctgcgct accttgccga tggcggcgcg agcgccgtcc tcaactgcgg ctatggccgc
     4201 ggggcgagcg tgcgcgaagt gctggcgatc gcccgcgagg tgaccggcgt ggactttccg
     4261 ctgcgcgagg ccgcacggcg gccgggagat ccggcgcggc tggtggccgc ggtggagcgt
     4321 atcggagcgg cgctccactg gaagccgcgg tacgcggatc tgcccaccat catcgcccac
     4381 gcctgggcct gggaacggca tctggccgct cgcaagcatt gaggatccaa gggcggggtc
     4441 ttgtgtcttc gcatcggctg cggcaagctg agcccaaagc ggcaaggagc agagacatgg
     4501 cgaagatctg ggtggtggta ggcgacaatt cccgggcgcg gatgtttgcg accaatcgcg
     4561 cggcgccgct cgaggagcgg gaggattacg tgcccgccta cgatcccggg caggacaagg
     4621 agcgattcac cgatcgcact gcgcatcagg gccacgtggc cgaaggcgga cgggatccgg
     4681 cggccgaggc cgatcgccgc ttcgttcgcc ggatcgccgg gcgcctgcgc gaagcccggc
     4741 tcgccgggga atacgagcgc ctcttcctgg tcgcgccgcc ggtatttctc ggcgaactgc
     4801 gccaggccct ggactcgggc acccgcgagg tcgtcgtagg tgaggcggcc aagaatctct
     4861 cccagctccg cgccgacgac atccgggcgc atctgcccga gctgttgtga acgactgccg
     4921 gacgcaggcg gcgattcatg gacgccggag cggactttcc ttagaatgaa accctggcgc
     4981 aaccgccatt cgggcccatg agcgcgacgc gcgaggagcg atgacaaagc gggtttacat
     5041 ccagacctgg ggttgtcaga tgaacgagta cgattccgcg aaaatgcggg acgtgctcgc
     5101 ggtggaagcg gactacgaac cggcggccag ccccggggaa gccgatctca tcctgctcaa
     5161 tacctgctcc gtgcgcgaaa aggcacagga aaaggtcttc tcgcagctcg ggcagtggcg
     5221 agcgctcaag gaagcgcggc cggaaatcct gatcggcgtt ggcggctgcg tcgcgagcca
     5281 ggaaggcgag gcgctgctgc gccgcgcgcc cttcgtcgat ctcgtcttcg gtccgcagac
     5341 cctgcaccgg cttccgggcc tgctggcgga gcgccgagcg agcggtcgtg cggtcgtcga
     5401 catttccttt ccggagatcg agaagttcga ccaccttccc gcgccgcgcg cggaagggcc
     5461 ctgcgccttc gtctcgatca tggaaggctg cagcaagtat tgcagcttct gcgtcgtccc
     5521 ctatacgcgc ggcgaagaaa tcagccgcga tttcgacggc gttctcgccg aagtatggga
     5581 actcgcggag cgcggcgtcc gcgaaatcac ctttctcggc cagaacgtca acgcctatcg
     5641 cggtcgccgc cacgacggcg cgacggcgga cctcgcaagc ctgattcatt acgcggcggc
     5701 gatcgagggc atcgagcgca tccgcttcac gacctcgcac cc
//
LOCUS       DASUJG010000141         3869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1631000, whole genome shotgun sequence.
ACCESSION   DASUJG010000141 DASUJG010000000
VERSION     DASUJG010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1631000"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..517
                     /locus_tag="VFX38_02945"
     CDS             92..517
                     /locus_tag="VFX38_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5313849.1"
                     /translation="MHEYLDFAARNWALVAALVVVLALLAADEIYRRLKGAQELTPAA
                     AVQLLNRGALVIDCRADEDYRSGHIVGARNLSLAELARRAPELKRKRPKPVLAIGTNA
                     REAGRAAGLLRRAGFESVFVLKGGLATWRKENLPLERPR"
     gene            604..1095
                     /gene="secB"
                     /locus_tag="VFX38_02950"
     CDS             604..1095
                     /gene="secB"
                     /locus_tag="VFX38_02950"
                     /inference="COORDINATES: protein motif:HMM:NF014599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEX5313850.1"
                     /translation="MTDLDQPTAGAPRVPTDTQRFLLERIYLKDCSFESPLSPTVFQE
                     PVDAPDAHVNLQTQINALADRPEAREVVLSVGVEARSGERSLFVCEVQMAAVVTLDGL
                     ASSEEGAILGARVPEALFPFARQAVCDLVVRGGFLPVMLQPIDFDAVYRQHASQQGRP
                     LDS"
     gene            1099..2088
                     /locus_tag="VFX38_02955"
     CDS             1099..2088
                     /locus_tag="VFX38_02955"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004140139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX5313851.1"
                     /translation="MPATITVLGAGAWGSALAVHLARSGQAVRLWAHRAEQAAELAGA
                     RENCRRLPGVPFPDSLVVKGDFPAALAGADYALVAVPSQAFAQTLVGLSASILPVFWA
                     TKGLDPASGRPLHEKAREILGAERGLGVLSGPSFAAEVARGLPTAVTVAATDGRLARR
                     FAALLHGPSFRAYVSTDLTGVELGGAAKNVIAIAAGISDGLGFGANARAGLITRGLAE
                     ILRLGRALGARSRTLTGLSGLGDLVLTATDDQSRNRRFGLALGRGTNAAAAEAEIGAA
                     IEGIPTARALTRLARELGVEMPIGEEIRAILDGARSPRAAVEALMSRRMKSER"
     gene            2166..3485
                     /locus_tag="VFX38_02960"
     CDS             2166..3485
                     /locus_tag="VFX38_02960"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5313852.1"
                     /translation="MRFYHVHHAPATVAARGPGLAQFAPLYAVILVAYIGYAMTVTIF
                     VPMLLDPATRLVPPDASRSLRTVIAGILMALYPTGQFFGSPIVGALSDRLGRKKVLSV
                     SLVCSMGFYAVIAIALWRQELWLLMGACFFAGLCEADVAIAQSSIADRAAPEERGRYF
                     GFIFAFLSLGYIVGPLGGGWLSTVAGYAAPFWAMLLLLALTFLAVRFFFRGSPPAHPD
                     DHASPLREFSNLRNVFTDRRIRALNLSNFLLFLGLFGFFRVITVVMYDAFGITSQNRM
                     MSYYAYLAAMGMIANIFFVGPLTRRFSLRAVTLVALAVGAAGYLLAGLPWGHRPFELL
                     WISPIAAIAPCIAIACACALLSVSVGPEEQGAVMGNNQALQNGAEAISAVGLPAIAAL
                     GMNSIFGSGLSLYVAAAMMLLSLAVLALARSKRVRADAAEGATPETT"
     gene            complement(3514..>3869)
                     /locus_tag="VFX38_02965"
     CDS             complement(3514..>3869)
                     /locus_tag="VFX38_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019558848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5313853.1"
                     /translation="GFYYAPTVLAGVRRGMTAAEEEIFGPVAAILRAGNEDDALALAN
                     ATPYGLGGSVWTRDPARGERFARALACGSAFVNGMVKSDPRLPFGGVKHSGYGRELSE
                     AGLHEFLNIKTLWIA"
BASE COUNT          575 a         1382 c         1237 g          675 t
ORIGIN      
        1 cactatagcc gagggccgcg cgcaacgccg gaaacgggcc gcgttcatga catactaatg
       61 cctcgccgat ctcgccgact ttttccgatt cgtgcacgaa tatctcgact ttgccgcccg
      121 taactgggct ctcgtcgccg cgctcgtcgt ggtgctggcg cttctcgccg ccgacgaaat
      181 ctaccggcgc ctgaagggcg cgcaggagtt gacgccggcc gcggcggtgc agctcctcaa
      241 tcgcggcgcg ctcgtcatcg attgccgggc ggacgaagac taccgcagcg gccacatcgt
      301 cggagcacgc aatctgtcgc tcgccgaact cgccagacgc gcgccggaac tcaagcgcaa
      361 acgcccgaag ccggttctcg cgatcgggac caacgcgcgc gaagcgggac gcgccgccgg
      421 cctcctgcgt cgcgccggct tcgagtccgt gtttgtgctg aagggcggac tcgccacctg
      481 gcgcaaggaa aatctgccgc tggaacgtcc cagataaatg cggcagggaa ctcgtccgcg
      541 atttgcggca atcttccgcc gctcgccgtt ttgcggcaaa cggccttaca ggaaaccaca
      601 gccatgaccg atctcgacca accgaccgcc ggcgctccgc gcgtgccaac cgacacgcaa
      661 cgcttcctgc tcgaacgcat ctatctcaag gattgctcct tcgagtcgcc gctttcgccc
      721 acggtgtttc aggagccggt ggacgcgccc gacgcgcacg tcaatctgca aacccagatc
      781 aacgcgctcg ccgaccgtcc ggaggcccgc gaagtggtgc tgagcgtcgg tgtcgaggcg
      841 cgcagcggcg agcgcagcct cttcgtctgc gaggtgcaga tggccgccgt cgtcacgctc
      901 gacggattgg cgagcagcga ggaaggcgcg atcctcggcg cgcgggtgcc cgaggccctc
      961 ttccccttcg cacgtcaggc ggtctgcgat ctggtggtcc gcggcggctt tcttcccgtg
     1021 atgctccagc cgatcgactt cgacgccgtg taccgccagc acgcgagcca gcagggacgt
     1081 ccgctggatt cctgagtcat gccggccacc atcaccgtgc tcggcgccgg tgcctgggga
     1141 agcgcgctcg ccgtgcatct cgcccgcagc ggccaggcgg tgcgtctgtg ggctcatcgc
     1201 gcggaacagg cagcggaact ggcaggcgcg cgcgagaatt gccgccgcct gcccggcgtg
     1261 cccttccccg actccctggt cgtaaagggc gacttccccg ccgctctcgc aggcgccgac
     1321 tatgccctgg tcgcggtgcc gagccaagcc ttcgcgcaga cgcttgtcgg cctctcggca
     1381 agcatccttc cggtgttctg ggctaccaag ggcctcgacc cggcgagcgg ccgaccgctt
     1441 catgagaagg cccgcgaaat tctcggcgcg gaacgcggac tcggcgtcct ctccggaccg
     1501 agctttgcgg ccgaagttgc gcgcggactg cccactgcgg tgaccgtagc cgcgaccgac
     1561 gggcggctcg cgaggcgctt cgcggcactg ctgcacgggc cgagtttccg tgcctacgtc
     1621 agcaccgatc tcaccggagt ggaactcggg ggagccgcaa agaacgtcat cgccatcgcc
     1681 gcgggcatca gcgacggact cggtttcggc gccaacgccc gcgccggtct catcacccgc
     1741 ggactcgccg agattctccg cctcggaagg gcgctcgggg cccgctcccg aaccctcacg
     1801 ggactttccg gtctcggcga tctggtgctc acggcgaccg acgaccagtc gcgcaatcgc
     1861 cgcttcgggc tcgcgctcgg acgcggcaca aacgccgccg ccgccgaggc cgaaatcggc
     1921 gccgccatcg aaggcattcc gacggcaagg gcgctcaccc gcctcgcccg cgagctcggg
     1981 gtcgagatgc cgatcggcga ggaaattcgc gctatcctcg acggtgcgcg cagcccgcgg
     2041 gcggcggtcg aagcgctcat gagccggcgc atgaaatcgg agcgctgaat cgcgcccgac
     2101 gcgaagccga agcaagaact gcctgcgtct ataattcgaa tgccggttcg acagaccgag
     2161 gcgccgtgcg tttctatcac gttcatcatg cgccggccac ggtggccgcg cgcggacccg
     2221 ggctcgcgca gttcgccccg ctctatgcgg tgatcctcgt cgcctacatc ggctatgcga
     2281 tgacggtcac catcttcgtg cccatgctgc tcgacccggc gacccgcctc gtgccgccgg
     2341 atgccagccg cagcctgcgc accgtgatcg ccggaatcct gatggcgctc tacccgaccg
     2401 gacagttctt cggctcgccg atcgtgggcg cgctttccga tcgcctcgga cgaaaaaagg
     2461 tgctgagcgt ctcgctcgtc tgcagcatgg gtttttatgc cgtcatcgcg atcgccctgt
     2521 ggcggcagga actgtggctg ctgatggggg cctgtttttt cgcgggcctg tgcgaggccg
     2581 acgtggcgat cgcgcaaagc agcatcgcgg atcgcgcggc gcccgaggag cgcggccgct
     2641 acttcggatt catcttcgcg tttttgagcc tcggctacat cgtcgggccg ctcggcggcg
     2701 gctggctgag cacggtcgcg ggctatgcgg ccccgttctg ggcaatgctg ctcctgctcg
     2761 cgctgacctt cctcgccgtg cgattctttt tccgcggctc cccgccggcg catcccgatg
     2821 atcacgcgag tccgttgcgg gaattttcga atctgcgcaa cgtcttcacc gaccggcgca
     2881 tccgcgcgct caacctctcg aactttctcc tgtttctcgg cctcttcggg ttctttcgcg
     2941 tcatcaccgt cgtgatgtac gacgccttcg gcatcaccag ccagaaccgg atgatgagct
     3001 attacgccta tctcgccgcc atgggcatga tcgccaacat ctttttcgtc ggcccgctca
     3061 cccgccgttt ttcgctgcgc gccgtcaccc tcgtcgctct cgccgtcggc gcggccggtt
     3121 accttctcgc gggcctgccc tggggccatc gtcccttcga gctgctgtgg atttccccga
     3181 tcgcggcgat cgcgccctgc atcgccatcg cctgcgcctg cgcactgctg agcgtctccg
     3241 tcgggcccga ggaacagggc gcggtgatgg gcaacaatca ggcgctccag aacggcgccg
     3301 aggcgatttc cgccgtgggc ctccccgcga tcgcggctct cggaatgaac agcatcttcg
     3361 gttcgggcct gtcactctac gtcgccgcgg cgatgatgct gctctcgctc gcggtgctcg
     3421 cgctcgcgcg aagcaagcgc gtgcgagccg atgccgcgga aggcgcaacg ccggagacta
     3481 cgtagcagca gccggcggcg gaccgactcc ggatcaggcg atccagagcg ttttgatgtt
     3541 gagaaattcg tgcagccccg cctcggagag ttcgcgtccg taaccggaat gctttacgcc
     3601 gccgaagggc aggcgcggat cgctcttcac catgccgttc acgaaggccg aaccgcaggc
     3661 gagtgcgcgc gcgaaacgct cgccgcgggc agggtcgcgc gtccacacgc tgccgccgag
     3721 tccgtaggga gtcgcgttcg cgagcgcgag cgcatcgtct tcgttcccgg ctcgcaggat
     3781 cgccgcgacc ggcccgaaga tttcttcctc ggcggccgtc atgccgcggc gcaccccggc
     3841 gagcaccgtc ggcgcgtaat agaaacccg
//
LOCUS       DASUJG010000142        10359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_41062, whole genome shotgun sequence.
ACCESSION   DASUJG010000142 DASUJG010000000
VERSION     DASUJG010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 10359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10359
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41062"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VFX38_02970"
     CDS             complement(<1..645)
                     /locus_tag="VFX38_02970"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX5313854.1"
                     /translation="MSWEAIFTLVVLGVTVIALGWPRMPPDLPLVGAMALLAVTGAAP
                     LKEVFEGFANPGLISVAALYVVAAGLQHTGAVTGPARWIFGRSRRLWISQLRVMVPTA
                     AISAFMNNTPVVAALMPAVLDWAGRRRIAASRLLMPLSFATILGGTCTLIGTSTNVIV
                     NGLLVATPGEHSMGFFTIGIVGLPVAIVGIAYMVIFGRRLLPDRRSALGELTDPR"
     gene            complement(642..3491)
                     /gene="uvrA"
                     /locus_tag="VFX38_02975"
     CDS             complement(642..3491)
                     /gene="uvrA"
                     /locus_tag="VFX38_02975"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX5313855.1"
                     /translation="MDAIRIRGARTHNLKNVDLDLPRERLIVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLALMEKPDADSIEGLSPAIAIEQKASSHNPRSTVGTVT
                     EIYDYLRLLYARAGTPRCPTHGIDLDAQTVSQMVDAVLARPEGERVMLLAPLVRARKG
                     EHAELLAQLKARGFLRVRVDGEVLELDEAGARLDRARAHTIELVVDRLRARADAAARL
                     AESFETALAFGDGNATVVSLDDSAREPLVFSARYACPVCGWSLAELKPRLFSFNNPAG
                     ACPVCDGLGAEQFFDPARIVARPTLSLAGGALRGWDRRNPYYFEPIRSLARHYEFDPE
                     TPFAKLPERIRGIVLYGSGEEEIRFEYLSSRGRRYTRAHAFEGIVRILERRYRETESL
                     AVREELARYRAIRPCPACGGARLNEAARSVFVAGRNIAQVVALDVLAAHDFFASLALP
                     GRRGEIAARIVREVESRLRFLRDVGLGYLTLARSAETLSGGEAQRIRLASQIGSGLTG
                     VMYVLDEPSIGLHQRDNRRLIETLLRLRDLGNTVIVVEHDEETIRAADFLVDMGPGAG
                     AHGGEIVALGTAAEIEAAPGSLTGDYLAGRRAIPVPPLRRIPSPEREIRIRGARGNNL
                     ANLDVAFPLGLLTCVTGVSGSGKSTLVSYTLEAAAARALNRANAAVAPCDAIEGLEHL
                     DKVIAIDQNPIGRTPRSNPATYTGLLAPVRELFAQTPEARARGYKPGRFSFNVRGGRC
                     EACQGDGLIKVEMHFLPDLYVPCDVCHGARYNRETLEVRYKGRNIHEVLEMTVEEASA
                     FFANVPAVRHRLETLAAVGLGYVRLGQSATTLSGGEAQRVKLARELARRDTGRVLYVL
                     DEPTTGLHFHDIRQLLAVLLRLREHGNSIVVIEHNLEVIKTADWIIDLGPEGGAGGGR
                     VVACGSPEAVAANPASHTGAFLARELARTASRTDG"
     gene            4001..6853
                     /locus_tag="VFX38_02980"
     CDS             4001..6853
                     /locus_tag="VFX38_02980"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEX5313856.1"
                     /translation="MALGVGLVVGGMVLPIVLCAGLVLGGVLRLPGFPSHPAFLTSAN
                     LGFAFKGAPGVFAPAPTSSGAFSMVNLMLRQADCSLTEDFVANLNLLTQSFTVQSAPD
                     VQDYLHAAAGLTTPPDVFPDGCPDSDRGVGDRYAVFLGKTGDGKQLVATFLLFRDLVI
                     FTGDLNGPWSETLVSLPDSSAAGDLNVGDVNGDGHADLIVPVIGERDTSGDGVVVVLN
                     HGDGSFSAPTFTPVPLLRGVTLDDFDGDGKLDLAGVTAATMPGGGGAVVLLPGKGDGT
                     FKTAIPGPSINSPYESFLASADFNGDGNRDLVLGDGEVLLGNGDGSFALDPHSIPVSG
                     LNPTGNFPAVADFNDDGKDDVALLATDGSVAIFLGNGDGSFSAASQYQYAGIAQAFTL
                     TATDLDGDGNADLVEGSANGGGFGADENTDGAFLMLMGRGDGSFVDSTVYPLSVSPTF
                     AVGDFNGDGKPDVLATQGSGPNVSGMMLLAGNGDGTFAPQPPDTAVQPLHVAAADMNG
                     DGNLDAVVIDQTQTHLGVLLANGNGGFGAPTSYPIPAGGQSGTTLTVGDFRGNGPPDV
                     IVGNGGDLLYYRNNGDGTLAAAQQFDQEDLVDATAVGDMNGDGNADLAVAIGNFEDAA
                     AGANNVVLVFLSNGDGTFAAPVRYAAGAYPRSIALGDLNQDGKPDLVVGSINAGLSAN
                     TLNVLLGNGDGTFGAALRSPLPYDAGSSSLAIADFDGDGNPDVAIGGGTYTFVSLGKG
                     DGTFSGNYASPLGSLAENLAVADLNHDQRPDLLLTSGSLGFAKLAALVNVKASATPPG
                     PGGTPTATTATLRASTTHLHTGQRVTFTATVAAAEGSVIPAGTVHFNDGDTTLGSAPL
                     SDSGTASFATSSLAAGSHSITAAYAGDSNFGASTSPPVTVTVIASTPPPTPPPGTPPS
                     SGGGAMNPLTVILLFALGAWVALKRRALHLRRPRDP"
     gene            complement(6929..7213)
                     /locus_tag="VFX38_02985"
     CDS             complement(6929..7213)
                     /locus_tag="VFX38_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313857.1"
                     /translation="MTLRIGRDRGAQVRSLWKGEGAIGKHGENSDQRNRCAADQRLAH
                     ETSSRCGEQGKGVSRHEPAMNPCGTVPERADEHSRTRSTVGRLPFAGGSE"
     gene            7339..8214
                     /locus_tag="VFX38_02990"
     CDS             7339..8214
                     /locus_tag="VFX38_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5313858.1"
                     /translation="MNDSTRKSPKGPAAEDPSRLPHVPYQPVLTGQPAIVTGANSGIG
                     RAVALGLAQAGADVAVNYVVHPETAEEVAHEIEGYGRKAIALQADVSKEDEVEAMYAA
                     AIQHFGTLHISVSNAGLQRDSGFENMTLEQWNTVIGVNLTGQFLCCRAAAREFQRRGV
                     VDKVSVAAGKMVCMSSVHQKIPWAGHANYAASKGGIMMMMQSIAQELAPYRIRVNAIA
                     PGAIRTPINTAAWETPEAYSSLMTLVPYKRIGEPDDIARAAVWLASDAADYVTGATLF
                     VDGGMTLFPGFATGG"
     gene            8221..9804
                     /locus_tag="VFX38_02995"
     CDS             8221..9804
                     /locus_tag="VFX38_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019997547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX5313859.1"
                     /translation="MADAHYDLIVVGSGPGGAALAQRLAPTGKRILMLERGDYLARSL
                     DNYSPHKVFIENVYQSDETWYDQHGTAFHPGLHYYVGGNSKMYGAALFRLRERDFEEL
                     QHAGGVSPEWPLKYDVFEPYYAEAERLFHVHGAAGEDPTEPRRGGPFPYPPVSHEPRM
                     QALADTFTRHGLHPFHMPLGILLDEKDGRVQPGSACFRCAYFDGYPCLTNGKADAQVI
                     CVDPTIARYPNVTLLTGAFVTRLDTDAAGRRVSAVNVERDGRSEQYSGDVVIVACGAL
                     SSALLLLRSANDQHPRGLANGSDQIGRNYMRHNQAALMAISKEPNDTIFQKTVGVSDF
                     YFGAEDWNFPLGLIQMTGKAHPDTIRGEALPSWLEWLPEMPFEKLARHSLDFWLSSED
                     LPRAENRIRYDGDRIVVEQTDNNMEALARLRRKLQSLLGPMGAHPHLLERRFYLGKDI
                     PIGGTAHQAGTARFGADPATSVLDLDCKAHELDNLYVTDASFFPSIGAVNPTLTIVAN
                     ALRVADRLAERLGATAADS"
     gene            9826..10347
                     /locus_tag="VFX38_03000"
     CDS             9826..10347
                     /locus_tag="VFX38_03000"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX5313860.1"
                     /translation="MDWLYVILVPLVIRICLVILFPFSALDKILDWNGAMAQAKSSFL
                     PTVLAAPMMVAAIVVEFVAPFLIVIGWHDRVAAFILAGFCVVTALLYHNFWHYRDFWV
                     RGDSRARAHFWDFLKNFGLVGGLLLVTFGTSVLPADWVARHAFSATPVYQRNAAPPGQ
                     ENCGAPGLPACRP"
BASE COUNT         1755 a         3433 c         3420 g         1751 t
ORIGIN      
        1 gcgcggatcg gtgagttcgc cgagcgcgct tctgcggtcg ggaaggagcc ggcgcccgaa
       61 gatcaccatg taggcgatgc cgacgatcgc gaccggcagt ccgacgatgc cgatggtgaa
      121 aaatcccatg ctgtgttcgc cgggagtggc gacgagcagt ccgttcacga tcacgttggt
      181 gctggtgccg atgagggtgc aggtgccgcc gaggatggtg gcgaaggaaa gcggcatgag
      241 caggcgcgag gcggcgatgc gccgccgccc cgcccagtcg agcacggccg gcatcagcgc
      301 cgcgaccacc ggcgtgttgt tcatgaaggc gctgatcgcc gccgtcggca ccatgacgcg
      361 aagctgggag atccacaatc ttcgcgagcg cccgaaaatc cagcgcgccg gccccgtgac
      421 cgcgccggtg tgctgcagac cggcggcgac gacgtagagc gcggcgaccg aaatgagacc
      481 ggggttggcg aatccctcga agacttcctt gagcggcgcg gccccggtca ccgcgagcag
      541 cgccatcgcg ccgacgagcg gcagatccgg cggcatgcgc ggccagccga gcgcgatcac
      601 cgtcacgccg agcacgacga gggtgaagat cgcctcccag ctcatccgtc ggtcctgctc
      661 gcggtccgcg cgagttcgcg ggcgagaaag gcgccggtat gcgaggcggg attcgcggcg
      721 acggcttccg gcgaaccgca ggcgacgacc cgtccgcccc ccgcgccgcc ttcggggccg
      781 agatcgatga tccagtccgc ggtcttgatc acttcgagat tgtgctcgat cacgacgatg
      841 ctgttgccgt gctcgcgcag gcgcagcagc accgcgagga gctggcggat gtcgtgaaag
      901 tgcagccccg tggtcggctc gtcgagcacg tagagtacac gaccggtgtc gcgccgggcg
      961 agttcgcggg cgagctttac gcgctgcgct tcgccgccgg agagcgtggt cgcgctctgg
     1021 ccgaggcgca cgtagccgag gccgacggcg gcgagcgtct cgaggcggtg gcgcaccgcg
     1081 gggacgttgg cgaaaaaggc gctcgcttcc tccaccgtca tctccagaac ctcgtgaatg
     1141 ttgcgcccct tgtagcgcac ctcgagcgtc tcgcggttgt agcgcgcgcc gtgacacacg
     1201 tcacagggaa cgtagagatc gggcagaaaa tgcatctcga ccttgatgag accgtcgccc
     1261 tggcaggcct cgcagcgtcc gccgcgcacg ttgaaggaga agcgtcccgg cttgtagccg
     1321 cgagcgcgcg cctcgggcgt ttgcgcgaac agttcgcgca ccggcgcgag cagcccggtg
     1381 taggtcgcgg gattcgaacg cggagtgcgc ccaatcgggt tctggtcgat cgcgatcacc
     1441 ttgtccagat gttcgagacc ctcgatcgca tcgcagggag cgacggcggc attcgcgcga
     1501 ttgagcgcgc gcgccgccgc cgcctccagg gtgtagctca cgagggtcga cttgcccgag
     1561 cccgaaactc cggtgacgca ggtgagaagt ccgagcggaa aggcgacatc gaggttcgcg
     1621 agattgttgc cgcgggcacc gcggatgcgg atttcgcgct ccggcgatgg gatgcgccgc
     1681 agcggcggca ccgggatcgc gcgccgcccg gcgagatagt cgccggtgag agagccggga
     1741 gcggcttcga tctcggccgc ggtgccgagc gcgacgattt cgccgccgtg ggcgcccgcg
     1801 cccggcccca tgtcgacgag gaaatcggcc gcgcgaatcg tttcctcgtc atgttcgacg
     1861 acgatcacgg tgttgccgag atcgcgcagc cgcagcaggg tttcgatgag gcggcgattg
     1921 tcgcgttgat gcaggccgat cgagggctcg tcgagcacgt acatcacgcc ggtgagtccc
     1981 gagccgatct ggctcgcgag gcggatgcgc tgcgcctcgc cgcccgaaag cgtctcggcg
     2041 ctgcgcgcga gggtcagata gccgaggcct acgtcgcgaa gaaaacgcag acgcgattcg
     2101 acttccctca cgatccgcgc ggcgatctcg ccgcgccgtc cgggaagcgc gaggctcgcg
     2161 aaaaaatcat gtgcggcgag aacgtcgagc gcgaccacct gcgcgatatt gcgcccggcg
     2221 acgaagacgc tgcgcgccgc ctcgttcagg cgcgcgccgc cgcaggcggg gcaggggcgg
     2281 atcgcacggt agcgcgcaag ttcctcgcgc acggccagcg attcggtctc gcgatagcgc
     2341 cgctcgagga tgcgcacgat cccttcgaag gcatgggcac gggtgtaccg ccgcccgcgc
     2401 gaggaaagat attcgaagcg gatttcctct tcgccgctgc cgtagagaac gatcccgcga
     2461 atgcgctccg ggagtttcgc gaagggcgtc tccggatcga attcgtaatg acgcgcgagg
     2521 ctgcggatgg gttcgaaata gtagggattg cgccggtccc agccgcggag cgcgccgccc
     2581 gcgagcgaaa gcgtcggacg ggcgacgatc cgcgcgggat cgaagaactg ctcggctccg
     2641 agtccgtcgc agacggggca ggcgccggcg ggattgttga aggagaaaag ccgcggcttg
     2701 agttcggcga gactccagcc gcagaccggg caggcgtagc gcgcggagaa gacgagaggt
     2761 tcgcgcgcgg aatcgtcgag gctcacgacg gtggcgttgc cgtcgccgaa ggcgagcgcc
     2821 gtctcgaagg attcggcgag gcgcgccgcc gcatcggcgc gcgcccgcag gcgatcgacg
     2881 accagctcaa tcgtgtgcgc acgggcgcga tcgaggcgcg cccccgcctc gtcgagttcg
     2941 agcacctcgc cgtcgacgcg cacgcggaga aatccccgcg ccttgagttg cgcgagaagc
     3001 tcggcgtgtt cgcccttgcg cgcgcgcacc agcggcgcga gcagcatcac gcgttcgccc
     3061 tcggggcggg cgagcaccgc gtccaccatc tgcgagacgg tctgcgcgtc gagatcgatg
     3121 ccgtgcgtcg ggcagcgcgg agtgccggcg cgggcgtaga gcagacgcag atagtcgtag
     3181 atctcggtga cggttccgac cgtcgagcgc ggattgtggg aactcgcctt ctgctcgatc
     3241 gcgatcgcgg gcgagagtcc ctcgatggaa tccgcgtccg gcttttccat cagcgcgaga
     3301 aactggcggg cgtaggccga gagcgattcc acgtagcggc gctggccttc ggcgtagagc
     3361 gtatcgaagg cgagcgagga ctttccggag ccggaaagac cggtgatgac gatcaggcgc
     3421 tcgcgcggca gatcgagatc cacgttcttg agattgtgcg tgcgcgcgcc gcggatgcgg
     3481 atggcgtcca tcgccgcccg gattggcgaa actgcctact atacgatggg gtcgcgggcc
     3541 gggcaatgcg cgcgatacag tacgttcaag gcctgtgaag cgcccgacgg gagtgccttg
     3601 gccagaaaca tgtccggctg tctccgggca aatgcatgcg ctcgccgatg gcgccgggcc
     3661 gatccggcga ttcggctcgc gtttcccgag aaatcccgga atacttcccg tttcgatatt
     3721 cgcgcgaaag caggaatcta tcaccatggt ttttctggat tcccgctttc gcgggcatga
     3781 cgaataaagc aaagacctcc caaatccgca aagctccgac ggcgcttcct ctccggcgcc
     3841 gaagattccg cgtgacgttt ctgcgctttc tctcacttac tgcaggagcg gaaaaccgcg
     3901 cgaccctttt tccaggaggt ggaccatgca accgaacatt cgcagcaaaa tttcccggat
     3961 cgctcgctcg attcgtggtc gcctcaaaag accgcgcctg atggcgcttg gcgtcggctt
     4021 ggtggtgggc ggaatggtgt tgcccatcgt gctttgcgcg ggcctcgtgc tgggaggagt
     4081 gctgcggctg cccggctttc cgtctcaccc ggcctttcta acctccgcaa accttgggtt
     4141 tgcgttcaag ggcgcccccg gggtgtttgc gccggcgccg acttcgagcg gcgcattctc
     4201 catggtcaac ttgatgttgc gccaggccga ttgttcgttg accgaagact ttgtcgccaa
     4261 cctcaatctg ctgacgcaaa gcttcacggt ccagagcgcc cccgacgtgc aggattacct
     4321 gcacgcggcg gccggcctca cgacgccgcc ggatgtgttt cccgacggct gcccggacag
     4381 cgatcgcggc gtcggcgacc ggtatgcggt gtttctgggc aagacgggcg acggcaagca
     4441 attagtggcc acctttttgc ttttcagaga tctagtcatc tttacgggcg acttgaacgg
     4501 cccgtggtcg gaaacgctgg tttcccttcc cgattccagc gctgccgggg atttgaacgt
     4561 gggcgatgtc aacggcgacg gccatgcgga tttgatcgta cccgtcattg gcgaacgcga
     4621 cacgagcggc gatggcgttg tcgtcgtgct caatcacggc gacgggagct tttcggcgcc
     4681 gacattcacg cccgttcctc ttctcagggg cgtgaccctg gatgacttcg acggcgacgg
     4741 caagctcgac cttgcgggcg tgacagcggc gacgatgccg ggcgggggcg gcgctgttgt
     4801 cctcctgccg ggcaagggcg acggcacgtt caagaccgcg attccggggc cgtcgatcaa
     4861 ctccccctac gaatctttcc tcgcgagcgc cgatttcaac ggcgacggca accgcgacct
     4921 cgtactcggc gacggcgagg tgctgctcgg caacggcgac ggcagtttcg cgctggaccc
     4981 acattcgata ccggtgtcgg gcctcaatcc caccggcaac tttccggcgg tcgcggattt
     5041 caatgatgac ggcaaggacg acgtcgcttt acttgcgact gatggcagcg tggccatctt
     5101 tctcggcaat ggcgacggca gcttctcggc cgcttcgcaa tatcagtatg ccggcatcgc
     5161 gcaagcgttc acgctcaccg ccaccgatct cgatggcgac ggcaatgcgg acctggtgga
     5221 aggcagcgcc aacggcggcg gcttcggtgc cgacgagaac acggacggcg ccttcctgat
     5281 gctgatgggc aggggtgacg gcagcttcgt tgactcgacc gtctatccac tcagcgtatc
     5341 accgacattc gccgtgggcg acttcaacgg cgatggcaaa cccgacgtgc tcgcgaccca
     5401 aggaagcggg cccaatgtca gcggcatgat gctgctcgcc ggcaacggcg acggcacatt
     5461 tgcgccacag ccgccggaca ccgccgtgca gccgctccac gtggcggcgg ccgacatgaa
     5521 cggcgacggc aacctggacg ccgtggtgat agaccagacg caaacgcatc tcggggtgct
     5581 gctcgccaac ggcaacggcg gcttcggcgc gccgacctcg tatccgattc ccgcaggcgg
     5641 acagtccggc accaccctga ccgtgggcga ctttcgcggc aacggcccgc cggacgtgat
     5701 cgtgggcaac ggcggcgatc tgctctatta ccggaataac ggcgacggca ccctggctgc
     5761 tgcgcagcag ttcgaccagg aagacctggt ggatgcaacg gcggtcggcg acatgaacgg
     5821 cgacggcaac gccgatctgg cggtggcgat cggaaatttc gaagatgctg ccgccggcgc
     5881 gaacaacgtc gtcctcgttt ttctgagcaa cggcgacgga acgttcgcgg cgccggtgcg
     5941 ctatgccgcc ggcgcgtatc cgcgaagtat cgcgctgggc gatctgaacc aggacggcaa
     6001 acccgatctg gtcgtaggct cgatcaatgc ggggttgagt gccaatacct tgaacgtgct
     6061 cctcgggaac ggcgatggaa ctttcggcgc ggcgctccgc tcgcctttgc catacgatgc
     6121 gggcagcagc agccttgcca ttgccgattt cgacggcgac ggcaatccgg acgtcgcaat
     6181 cggcggcggg acttacactt tcgtgtctct gggcaagggc gacgggacct tttccggtaa
     6241 ctatgcctca ccgctcggct ccctcgcgga gaatcttgcc gtcgccgatc tgaaccatga
     6301 ccagcggccc gacttgctgc tgacctccgg gagcctgggt ttcgccaaac tcgcggcctt
     6361 ggtcaacgtc aaggcaagcg cgacgccgcc aggcccgggc ggaacgccca cggcgacaac
     6421 cgcgacgctt cgagcttcca cgacccatct ccataccgga caacgcgtga ccttcaccgc
     6481 tacggtcgcc gccgccgagg gctctgtcat tcccgccggc acggtccact tcaacgacgg
     6541 cgacaccacg ctcggaagcg cccccctgtc ggattcgggg accgcaagct tcgccaccag
     6601 ttcgctcgcg gccggcagcc attccattac cgcggcctac gcgggcgact ccaatttcgg
     6661 cgcctcgacc tcgccgccgg tcaccgtcac cgtgatcgcg tccacgccgc cgccgactcc
     6721 gccgccgggc acgcccccgt catcgggcgg cggcgcgatg aatccgctga ccgtcatcct
     6781 gctgttcgcc ctgggtgcgt gggtggcgct caaacgccgg gcgctgcatc ttcgccggcc
     6841 aagagaccct tgaaggacaa ccctgacgcc tgttgccgga atcggttacc gcccgacggc
     6901 cggataaaaa atatcgaggg gttctgaatc attcggagcc tcctgcaaac ggtagacggc
     6961 cgaccgtcga tcgggtacgc gagtgctcat cggcgcgttc cggaacggtc ccgcacgggt
     7021 tcatcgccgg ttcgtgccgg gaaacgcctt tgccttgttc gccgcatcgc gacgatgttt
     7081 cgtgcgccag ccgctgatcg gcggcgcagc ggtttcgctg gtcggaattt tcgccgtgct
     7141 tgcctatcgc gccttcgcct ttccacagcg agcggacctg agcaccacgg tcgcgcccga
     7201 ttcgcagcgt cacagagcta tgcgttatcc ttgcagtccc acccgcggtt ggcttccggc
     7261 gcgaagtcga acaagcccgc ttcccggcgc aaggtcagcc cgaaagcgaa taatccatca
     7321 tcccgaccgg agaatccgat gaacgattcc acacgcaaaa gcccgaaggg cccggccgcc
     7381 gaggatccct cgcgcctgcc gcacgttccg tatcagccgg ttctcaccgg gcagcccgcg
     7441 atcgtgaccg gcgcgaattc cggcatcggg cgcgccgtcg ctctcgggct cgcgcaggcg
     7501 ggcgccgacg tcgccgtgaa ctacgtcgtc catcccgaaa ccgcggagga ggtcgcgcac
     7561 gagatcgagg gatacgggcg caaggcgatc gcgctccagg ccgatgtcag caaggaggac
     7621 gaggtcgagg cgatgtatgc cgccgcaatc cagcacttcg ggactctgca tatttcggtg
     7681 agcaatgccg gattgcagcg cgattccggg ttcgaaaaca tgacactcga acaatggaac
     7741 accgtgatcg gggtgaatct gaccggccag ttcctctgct gtcgcgcggc ggcgagggaa
     7801 ttccagcgcc gcggggtggt ggacaaggta tcggtcgccg cgggcaagat ggtgtgcatg
     7861 agttcggtgc accagaagat tccctgggcg gggcacgcga actacgccgc ttccaagggc
     7921 ggcatcatga tgatgatgca gagcatcgcg caggaactcg cgccgtatcg catccgcgtg
     7981 aatgcgatcg cgccgggggc gattcgcacg cccatcaaca ccgccgcctg ggagacgccc
     8041 gaggcgtatt cgagcctgat gacgctcgta ccctacaagc gcatcggcga acccgacgac
     8101 atcgctcgcg ccgcggtgtg gctcgcctcg gatgccgcgg attacgtgac cggcgcaacg
     8161 ctcttcgtgg acggcggaat gacgctcttc ccgggattcg caaccggagg ctgagcggcg
     8221 atggccgacg cgcactacga tctgatcgtc gtcggaagcg gccccggcgg cgcggcgctc
     8281 gcccagcgcc tcgcgcccac cggcaagcgc atcctgatgc tcgagcgcgg cgattatctc
     8341 gcgcgctcgc tcgacaacta cagcccgcac aaggtcttca tcgagaacgt gtaccagagc
     8401 gacgaaacct ggtacgacca gcacggcacc gcctttcatc ccgggctcca ctattacgtc
     8461 ggcggcaatt ccaagatgta cggcgccgca ctctttcgcc tgcgtgaacg cgacttcgag
     8521 gagctgcaac acgcgggcgg cgtttctccg gaatggccgc tcaagtacga cgtcttcgag
     8581 ccgtattacg cggaggcgga gcggctcttt cacgtgcacg gggcggcggg cgaggatccg
     8641 accgaaccgc ggcgcggcgg cccgtttccg tatccgccgg tttcgcacga gccgcgcatg
     8701 caggcgctcg ccgacacctt cactcgccac ggtctccatc cctttcacat gccgctcggg
     8761 attctgctcg acgaaaagga cggccgggtg cagccgggga gcgcctgctt ccgctgcgcc
     8821 tatttcgacg gctatccctg cctcaccaac ggcaaggccg acgcgcaggt gatctgcgtg
     8881 gacccgacca tcgcacgcta tccgaacgtc acgctcctca ccggcgcctt cgtcacccgt
     8941 ctcgacaccg acgccgccgg gcggcgcgtg agcgccgtca acgtcgagcg cgacggacgg
     9001 agcgaacagt actcgggcga tgtcgtgatc gtcgcctgcg gcgcgctctc ctcggcgctg
     9061 ctgctgctgc gctcggcgaa cgaccaacat ccgcgcggac tcgcgaacgg ctcggatcag
     9121 atcgggcgca attacatgcg ccacaatcag gcggcgctga tggcgatctc caaagagccg
     9181 aacgacacga tattccagaa aaccgtcggc gtgagcgact tctacttcgg cgccgaggac
     9241 tggaactttc ctctcggcct catccagatg accggcaagg cgcatcccga cacgatccgc
     9301 ggcgaggcgc ttccgtcctg gctcgaatgg ctgcccgaga tgcccttcga gaagctcgct
     9361 cgccattcgc tcgatttctg gctttcgagc gaggatctgc cgcgcgccga aaaccgcatc
     9421 cgctacgacg gcgatcgcat cgtcgtcgag cagaccgaca acaacatgga ggcgcttgcg
     9481 cgcctgcggc gcaagctcca atcactgctc gggccgatgg gtgcgcatcc gcatctgctc
     9541 gaacggcgct tctatctcgg caaggacatc ccgatcggcg gcaccgcgca tcaggcgggc
     9601 acggcgcgct tcggcgccga tcccgcgact tcggtgctcg acctcgactg caaggcgcac
     9661 gagctggaca acctctacgt caccgatgcg agtttctttc cctcgatcgg agccgtgaac
     9721 ccgacgctca ccatcgtcgc caatgcactg cgcgtcgccg atcgtctcgc cgaacggctc
     9781 ggagccaccg ccgccgattc ctgaacccgc tgcaaggagt gaacgatgga ctggttgtac
     9841 gtgattctcg tgcccctcgt gatccgcatc tgtctcgtca ttctgtttcc cttcagcgcg
     9901 ctcgacaaga ttctcgactg gaacggcgca atggcgcagg cgaaatcgag ttttcttccg
     9961 accgtgctcg cggcgccgat gatggtggcg gcgatcgtgg tcgaattcgt cgcgccgttc
    10021 ctgatcgtca tcggctggca cgatcgcgtc gccgccttca tcctcgccgg cttctgcgtc
    10081 gtgacggcgc ttctctatca caacttctgg cattaccgtg acttctgggt gcgcggcgac
    10141 agcagggccc gcgcgcactt ctgggatttc ctgaagaact tcggcttggt cggtggcctt
    10201 cttctcgtca ccttcggtac ctcggtgctg ccggccgact gggtggcccg ccacgcgttt
    10261 tccgcgactc cggtgtatca acgcaatgcg gcgccgcccg gacaggagaa ttgcggcgcc
    10321 ccgggactgc cggcctgccg cccatgagcg cggaagaca
//
LOCUS       DASUJG010000143         3607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_775085, whole genome shotgun sequence.
ACCESSION   DASUJG010000143 DASUJG010000000
VERSION     DASUJG010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775085"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2751)
                     /locus_tag="VFX38_03005"
     CDS             complement(<1..2751)
                     /locus_tag="VFX38_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEX5313861.1"
                     /translation="MQGKLFTQDFLERGISETAAWRGCPAEAVARCGRTLERLLRGVP
                     ATADWNEATTETELIEPVLTALGWNDFLKQQTTSRRGRADVPDYLLFPNGEAKRKALE
                     GPEDRRYRHGASILEAKRWERPLDRAERGDTFDPVTPSNQILRYLSRADVASERRIKW
                     GILTNGRYWRLYYQDARARAEDFVEFDLMALAKTAGAQGDLLNPGYTRPEHFLKVFYL
                     IFRREAFLSQPGDGAARSFHLLALDESRHWEMRVSEDLGHRVFEQIFPRLARALAASD
                     PARPATPDAAWLSQLNEETLTLLYRLLFLLYAEDRNLLPVHDPRYDDYSLRAIREDIA
                     RRIDDHDVFSASAARYYSHLRDLFHAIDAGDDSVGIPPYNGGLFEGRNHPLLARARIA
                     DAELAPLLDLLSRAGDAGERKWINYRDLSVQQLGSIYERLLERALVVDENGEIAVRLS
                     IYGRKGTGTYYTHDDLVKLIVREAVGPLVHERLQRFGATASERAVSRRSRAERLHALR
                     EVDAASKLLELRVCDPAIGSGHFLVALVDYLADEVLEAMAGATAEIAWAPESEPYESP
                     LAGRIAEIRGRLLESAKQHNWTLDPAQLDDRHIVRRMILKRVVHGVDKNPMAVELAKL
                     SLWLHTFTVGAPLSFLDHHLQAGDALFGERLERVLDELRARGAIFAQNTLTRIGSAAD
                     SMARIADLTDVDIAEVKQSEALFGGVVRDMEPLKLLLDFWQALRWRWPNGWPRDDDPE
                     ARALRELVGGNFGDLIEVVAAGHVTAATSAGMPVAEAAQKLLDDLLGLARRERFFHWE
                     LAFPGVWKDLVEGRPHGGFDAVIGNPPWDRMKLQEVEWFADRRPAIALEGRAADRKRA
                     IAELEAAQDPLWSQYLDARDRAETAARVARACGDYPLLASGDINLYSLFVERARSLVK
                     PEG"
     gene            complement(2967..3416)
                     /locus_tag="VFX38_03010"
     CDS             complement(2967..3416)
                     /locus_tag="VFX38_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313862.1"
                     /translation="MAESSSPGLNSAQARHVLATFRHVDALLDRVTAQFQPRNDGDST
                     AAIRNDVSAAQAAALCEAVAGVRAELEKGAEALDLPATAPAVSARWVAATSARLALIA
                     LAELDARQLAAYGAITGESAARVARVRERLEERISALIGAVDQGQQT"
     gene            complement(3389..>3607)
                     /locus_tag="VFX38_03015"
     CDS             complement(3389..>3607)
                     /locus_tag="VFX38_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5313863.1"
                     /translation="RAEVARTVPEGRRGEAFGILDTAMGAAWFAGSIVFGALYEISPA
                     ALAVAAAAIECAALLWMILAWPSLRRPA"
BASE COUNT          612 a         1159 c         1222 g          614 t
ORIGIN      
        1 gccttcgggt ttgacgagcg aacgggcgcg ctccacgaac agcgaataga ggttgatgtc
       61 gccgctggcg agcaaggggt agtcgccgca tgcgcgagca acgcgcgccg cagtttcggc
      121 gcgatcccgg gcatcgagat attgtgacca gagcggatcc tgcgccgctt cgagttcggc
      181 gatggcgcgc ttgcggtcgg cggcccggcc ttcgagggcg atcgcggggc ggcggtcggc
      241 gaaccattcg acttcctgca atttcattcg gtcccagggc ggattgccga tgacggcgtc
      301 gaagccgccg tgcgggcggc cttcgacgag atccttccat acgccgggaa aggccagctc
      361 ccaatggaag aaacgctcgc gccgcgcgag cccgagaaga tcgtccaaca gcttttgcgc
      421 ggcctccgcg accggcatac ccgcgctggt tgcagcggta acgtggccag ccgcaaccac
      481 ttcgatgaga tcgccgaaat tgccgccaac cagttcgcgc agcgcccgtg cttcgggatc
      541 gtcgtccctc ggccaaccgt tcggccagcg ccaacgcagc gcctgccaga agtcgagcag
      601 aagcttgagc ggttccatgt cgcggacgac gccgccgaac agcgcctcgg attgcttgac
      661 ctcggcgatg tccacgtcgg tgaggtccgc gatccgcgcc atgctgtcgg ccgccgagcc
      721 gatgcgagtc agggtgttct gcgcaaaaat cgcgccgcgc gcgcgaagct catcgagcac
      781 gcgctccagg cgctcgccga acagggcgtc gccggcctgc agatgatgat ccagaaagga
      841 cagcggcgca ccgacggtga aggtatgcag ccacagtgag agcttggcca gttcgacggc
      901 catgggattc ttgtccacgc cgtgcacgac gcgcttgagg atcatgcggc gcacgatgtg
      961 gcgatcgtcg agctgcgccg ggtccaacgt ccagttgtgc tgcttggcgg attcgagcag
     1021 ccggccgcgg atttcggcga tgcggcccgc gagcggcgat tcgtaaggct cgctttcggg
     1081 cgcccatgcg atttccgcgg tcgccccggc catcgcctcc agaacttcgt cggcaaggta
     1141 atccaccaat gccacgagga agtgaccgct gccgatcgcc ggatcgcaca cccggagttc
     1201 gaggagtttc gaggcggcgt ccacttcgcg tagcgcgtga agtcgctccg ctcgggaacg
     1261 gcgcgaaacc gcccgttcgg acgcggtggc gccgaagcgc tgcaggcgct cgtgcacgag
     1321 cgggccgacg gcttcccgaa cgatgagttt caccagatcg tcgtgggtgt aataggtgcc
     1381 ggttcccttg cgtccgtata tggacaggcg cacggcgatt tcgccatttt cgtcgacgac
     1441 cagcgcgcgt tcgagcagtc gttcatagat cgaaccgagt tgctgcacgg agagatcgcg
     1501 gtagttgatc cacttgcgtt cgcccgcgtc gccggcgcgg gacagaagat cgagcagcgg
     1561 tgccagttcg gcatccgcga tccgcgctct tgcgagcagc ggatggttgc gcccttcgaa
     1621 caatccgccg ttgtagggcg ggatgccgac ggaatcgtcg cccgcgtcta tggcgtgaaa
     1681 aagatcgcgc aggtggctgt agtaacgtgc ggcgctcgcg gagaaaacgt cgtgatcgtc
     1741 aatccggcgg gcaatgtcct cgcgaatggc gcgcagcgaa tagtcgtcgt agcgcgggtc
     1801 gtggacgggg agcaggttgc ggtcttcggc gtacagcaga aacagcagcc ggtacaaaag
     1861 cgtgagcgtc tcttcgttga gttgcgagag ccaggccgca tcgggcgttg cgggtcgcgc
     1921 aggatcggac gcggccagag cccgcgcgag gcgcgggaaa atctgttcga agacccggtg
     1981 tcccaaatcc tcggacacgc gcatttccca atgcctgctt tcgtcgaggg caaggagatg
     2041 gaaggaacgt gctgccccat cgccgggctg agacagaaac gcttcgcgcc ggaaaatcag
     2101 gtagaagacc ttgaggaaat gttcggggcg ggtatatccg gggttcagaa gatcgccctg
     2161 cgcgcccgcg gttttcgcca gcgccatcag gtcgaactcg acaaaatcct cggctctggc
     2221 gcgggcatcc tgatagtaaa gccgccagta acgcccgttg gtcagaatgc cccatttgat
     2281 gcgtcgttcg gaggcgacgt ccgcgcggct caggtagcgg agaatctggt tcgacggcgt
     2341 gacgggatcg aatgtgtccc cgcgctcggc gcgatccagc gggcgctccc accgtttggc
     2401 ctcaaggatc gaggcgccgt ggcggtagcg gcggtcctcc gggccttcga gcgccttgcg
     2461 ctttgcctcc ccgttcggga acagaagata atcggggacg tccgcccggc cgcgccgcga
     2521 ggtggtctgc tgcttcagga agtcgttcca tcccagcgcc gtcaggacgg gttcgatcag
     2581 ttcggtttcg gtggtggctt cgttccaatc ggccgtggcc gggacgccac ggagcaggcg
     2641 ctccagcgtc cggccgcagc gcgccacggc ctcggcgggg catccccgcc atgcggcggt
     2701 ttccgatatc ccccgctcca gaaagtcttg ggtgaacagt tttccctgca tccggcgccg
     2761 atctcggtag ggttgttctg cgtaacccgg gaaaggatac cagcgttgcg cccgggggca
     2821 atgtttcgcc gcttccgcag gggcacgatc gcgcacggac tgcggctgat tcgcaatgcg
     2881 ctacgtatgg caggccggca caagaccgca ggtccttgcc ggcaatccac agtccgactc
     2941 gccgggaacg tcgcgcctgt cccggcctac gtttgttgtc cttgatccac ggcgccgatc
     3001 agtgcggaga ttctttcttc cagtcgttcg cgtacgcgcg ccacgcgcgc ggccgactcg
     3061 cccgttatcg cgccgtaggc ggcaagttgc cgcgcatcca gttcggcaag cgcgatgagc
     3121 gcaagccgcg ccgacgttgc cgccacccag cgcgccgaaa ccgccggtgc cgtcgccggg
     3181 agatcgagcg cctctgcgcc cttttccagt tcggcacgca cgccggcgac cgcctcgcac
     3241 agcgcagcag cctgcgccgc ggacacgtcg ttgcgaatcg ccgcggtcga atcaccatcg
     3301 ttccgcggct ggaattgggc cgtcacacgg tcgagcagtg cgtccacgtg acgaaacgtg
     3361 gcgagcacgt gacgcgcctg cgcggagttc aggccgggcg acgaagactc ggccatgcca
     3421 agatcatcca taagagcgcc gcgcattcga tggcggcggc ggcaacggcc agcgcagccg
     3481 ggctgatctc gtaaagcgca ccgaacacga tgctgccggc gaaccaggcg gcgcccatcg
     3541 cggtgtcgag gatgccgaag gcttcgccgc gccggccttc gggcacggtt cgggctactt
     3601 cggcacg
//
LOCUS       DASUJG010000144         2855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_775330, whole genome shotgun sequence.
ACCESSION   DASUJG010000144 DASUJG010000000
VERSION     DASUJG010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775330"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VFX38_03020"
     CDS             <1..408
                     /locus_tag="VFX38_03020"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HEX5313864.1"
                     /translation="DPSLKRFIDNSYGYAVFPTVSSGAFIVGGSYGEGAAFHGSQMVA
                     KCSISKGSIGAQVGGQAYSEVIFFQSKPYFDNFVNGNFTFSAEISAVALTAGAAANVA
                     YNNGVAVFVLPKGGLMASAAVGGQKFSCQPASQ"
     gene            630..1022
                     /locus_tag="VFX38_03025"
     CDS             630..1022
                     /locus_tag="VFX38_03025"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEX5313865.1"
                     /translation="MRKALVSLLAIQVFVILAVAAGFYVYGREPAAVAAALYGGAVAL
                     GASLLLAWRMSRAGRAQAALNLGVLVLGLLERIVFIVAALIVGFAVLRLVPLALIAGF
                     VGGELAHYVAAGPLRRHILKQSEGNSGG"
     gene            1012..1881
                     /gene="atpB"
                     /locus_tag="VFX38_03030"
     CDS             1012..1881
                     /gene="atpB"
                     /locus_tag="VFX38_03030"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX5313866.1"
                     /translation="MAVEGGEGAGEYITHHLTNWCWGCNPQTHQPNGLVDFHAFNLDV
                     ILMSVLLAAGLVAFAWFMRRRWSAGKPGRLQYSLEFIIEYIGDQIREVFPKANNYVGA
                     MALTIFVWVFLMNAMDLIPIDLIPSIVGGIHSATGAGTAYFRDVPTATLDVPFAMAIV
                     IFVLMVVYQIRVNGPGGYAKRFLFHPYGKFGAPMNIVTTLIDDLAKPVSLALRLFGNM
                     FAGELIFALLALLTLSALQPPSLSAVGWIPANIVLGIVWTVFDLLIATLQAFIFGVLA
                     IVYLGMAQQTADH"
     gene            1925..2227
                     /gene="atpE"
                     /locus_tag="VFX38_03035"
     CDS             1925..2227
                     /gene="atpE"
                     /locus_tag="VFX38_03035"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEX5313867.1"
                     /translation="MNPNLIAEIVTGTAIGVGAMFAAAGLGSAIGWGLICSKTLEGIA
                     RQPEMQGKLMVNMFIFAGLMESFPFILFGVAMWFLFANPFVGAFTKFVSTAAGAGQ"
     gene            2256..2738
                     /locus_tag="VFX38_03040"
     CDS             2256..2738
                     /locus_tag="VFX38_03040"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004411.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEX5313868.1"
                     /translation="MVAITGTLIVQIVVFLVTIWLLKRLGWAWLVGQIDERRKRIAEG
                     IAAGEEGRRALAEAESQAAKAIEEARARATEIIDNANRRAREVVDAARVEAEGERGRE
                     LERARAEIEQAARQARENLRGDFARISAAAAGRILNEELDPAKHAALVDEFAEKLFHG
                     "
     gene            2731..>2855
                     /locus_tag="VFX38_03045"
     CDS             2731..>2855
                     /locus_tag="VFX38_03045"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011514636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEX5313869.1"
                     /translation="MADAATLARPYARAAFEVARERSALAEWQAFLRRLADLVALA"
BASE COUNT          454 a          887 c          937 g          577 t
ORIGIN      
        1 gatccgagcc tcaagcgctt catcgacaat tcctacggct atgccgtatt ccccaccgtg
       61 agttcgggcg cattcatcgt cggcggctcc tacggcgagg gcgcggcctt ccacggcagc
      121 cagatggtcg ccaagtgcag catttccaag ggttcgatcg gtgcccaggt cggcggccag
      181 gcgtacagcg aggtcatttt cttccagtcg aagccctatt tcgacaactt cgtaaacggg
      241 aacttcacgt tttccgccga aatctcggcc gtggccctca ccgccggcgc ggccgccaat
      301 gtggcctaca acaacggcgt cgccgtgttc gtgctgccca agggcggact catggcttcc
      361 gcggcggtcg gcggccagaa gttttcctgc cagccggcct cgcaataggc attcgcccgc
      421 aacccgcgga gcgtcaaagg ctccgcgggt tgcggagcgc gcgcggcggt ccgttcgcgc
      481 ggaatgccgc gagtttgcgg cgctccatcg cttttgatat cgggcgcggc gatttttcga
      541 cgccttcacg ttgaacggat gcgcgtgaac gggtaaaata cccgccgtcc gggaagcaca
      601 ggcgcgaagg ccgcgccgcc ccgacgcgga tgcggaaagc ccttgtcagc ctgctcgcga
      661 ttcaggtctt cgtgattctg gcggtcgcgg caggttttta tgtgtatggc cgcgagcccg
      721 ccgcagtagc ggcggcgctc tacggcggag cggtggccct gggggcgtcg ctcctgctcg
      781 cctggcggat gagccgcgcg gggcgggcgc aggcggcgtt gaacctgggc gtactggtcc
      841 tcgggttgct ggagcgcatc gtcttcatcg tggccgcact catcgtcggt ttcgcggtgt
      901 tgcggctcgt tccgctcgcc ttgatcgcgg gcttcgtggg cggggaactc gctcattatg
      961 tggcggccgg ccccttgcgc cggcacattc tgaaacagtc ggagggaaac agtggcggtt
     1021 gaaggcggcg aaggcgcggg cgaatacatc acccatcacc tgaccaactg gtgctggggc
     1081 tgcaatccgc agacgcatca gccgaacggg ctggtggatt ttcacgcctt caacctggac
     1141 gtgattctca tgtcggtgct tctcgccgct ggccttgtcg cgtttgcatg gttcatgcgc
     1201 cgacgctgga gcgcaggcaa gcccgggcgc ctccaatact cgctcgaatt catcatcgaa
     1261 tacatcggcg atcagattcg tgaagtattt ccgaaggcga acaattacgt cggcgcgatg
     1321 gcgctcacga ttttcgtctg ggtgtttctg atgaatgcga tggatctgat ccccatcgat
     1381 ctgatcccgt cgatcgttgg cgggattcac agcgctaccg gtgcaggaac cgcctatttt
     1441 cgcgacgtac ccacggccac gctggacgtg cccttcgcga tggcgatcgt gatcttcgtg
     1501 ctgatggtcg tttatcagat ccgggtgaac ggcccgggtg gctacgcgaa gcgctttctg
     1561 tttcatccgt acggaaagtt cggtgcgccg atgaatatcg tcacgaccct gatcgacgat
     1621 ctcgcgaagc cggtgagcct cgcgctgcga ctgttcggca acatgttcgc cggcgaactc
     1681 atcttcgcgc tgctcgcgct gctgacgctc tcggcgcttc agccgccctc tttgagcgcc
     1741 gtggggtgga tcccggcgaa catagttttg ggcatcgtct ggaccgtctt cgatcttctg
     1801 atcgcgacgc tccaggcatt cattttcggc gtgctcgcga tcgtctatct gggcatggcc
     1861 cagcagacgg cggaccatta ggaatttttg cgtccctggt tgtttttttg aggagtagag
     1921 caacgtgaat ccaaatttga ttgcggaaat cgtcaccggc acggccatcg gcgtcggcgc
     1981 catgttcgcc gccgccgggt tgggctcggc catcggctgg ggtctgattt gttccaagac
     2041 cctggaaggg atcgcgcgcc agccggagat gcagggcaaa ctgatggtga acatgttcat
     2101 ctttgcggga ctgatggaat ccttcccgtt cattctcttc ggggtggcca tgtggttcct
     2161 gttcgcgaac cccttcgtcg gcgccttcac caagttcgtg agcaccgccg ccggagcggg
     2221 ccagtaggcg cgagcggaac cgaggagaga acgccatggt agccatcacc ggaaccctca
     2281 tcgtccagat tgtcgttttt ctggtcacga tctggcttct caagcgtttg gggtgggctt
     2341 ggctcgtcgg ccagatagac gagcgtcgga agcgcatcgc cgagggcatc gccgcgggcg
     2401 aggagggacg gcgggcactc gccgaggccg agtcgcaggc ggcgaaggcg atcgaggagg
     2461 cgcgagcgcg ggcgaccgag atcatcgaca acgccaatcg ccgcgcccgc gaagtcgtgg
     2521 acgctgcccg ggtcgaggcc gaaggcgaac gcggccgcga actcgagcgc gcccgcgccg
     2581 agatcgagca ggccgcgcgc caggcgcgcg agaatctgcg cggcgacttc gcgcgcatct
     2641 ccgccgccgc cgccgggcgc atcctgaacg aggaactcga tccggcgaag cacgcggcac
     2701 tcgtggacga attcgcggag aagctcttcc atggctgatg ccgcgaccct cgcccggcct
     2761 tacgcgcgcg ccgccttcga ggtcgcgcgc gaacgaagtg cgctcgccga atggcaggcg
     2821 tttcttcgcc gtctcgccga tctggttgcg cttgc
//
LOCUS       DASUJG010000145         4173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_898842, whole genome shotgun sequence.
ACCESSION   DASUJG010000145 DASUJG010000000
VERSION     DASUJG010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898842"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="VFX38_03050"
     CDS             complement(<1..532)
                     /locus_tag="VFX38_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX5313870.1"
                     /translation="MLDDALHAPRGAHGRAPDPLRVGGWMPMRQYREDEEVDFAIVGT
                     GAGGGTLACKLAEYGFSVVGLDAGAWWRPLEEFASDETHQGKLFWTDERICDGEAPLK
                     LGSNNSGKAVGGSTVHFAMVSLRFRPEWFKSRSALGYGADWPLDWREMWRYYTEVEQA
                     LAIAGPIHYPWGPHRPR"
     gene            complement(522..1163)
                     /locus_tag="VFX38_03055"
     CDS             complement(522..1163)
                     /locus_tag="VFX38_03055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEX5313871.1"
                     /translation="MPERYPGYDVLAKRDSPSWNEPTRRVIAERLAIADEPHFFTTAE
                     WRTLTAICARIVPQPPTRTPVPIAAMVDRKLTRDARDGYRQASLPPLREAWRRGLAAL
                     DAAARERHETEFHVLPTSKQDALLKAMQQGELTGPEWGGMPADTFFNERLAHDILAAY
                     YSHPTAWNEIGFGGPASPRGYVRMKANRRDPWEGVEATPGREAEARRKNERVG"
     gene            complement(1156..2106)
                     /locus_tag="VFX38_03060"
     CDS             complement(1156..2106)
                     /locus_tag="VFX38_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313872.1"
                     /translation="MLTDRQIRNCVLGIVALGVGSTLLRRDEAPRGKEKRPRKKSARA
                     PSPPTVKAARRLNRAAGLIATSTLLDSAIEHYRGSFQNKAMFTPLIVSALAVGASAHG
                     TGDKRPAVHKFRDMTYLASALTGLVGTGFHIYNIAKRPGGFSWQNLFYGSPLGAPAAV
                     ALAGLLGYYSERLRENESGRTPKVFGMPAGRALAALSGLGLLATVGEAGLLHFRGAYH
                     DPFMYLPVTVPPIAAVLLGETAASRPRRFTRLWLRLTALLGFAGAGFHIWGVHRNMGG
                     WHNWRQNVLNGPPIPAPPSFTGLALAGLAALRLLEDHPDA"
     gene            complement(2482..2769)
                     /locus_tag="VFX38_03065"
     CDS             complement(2482..2769)
                     /locus_tag="VFX38_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012749752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX5313873.1"
                     /translation="MAQNKLYVGNFPYSVDEAQLRGIFSQYGEISELALIMDRDSGRP
                     KGFGFITFATQAAAEKALEQNGKDLGGRPLKVNVAMDKPRSNGGGRSGSRW"
     gene            complement(3255..>4173)
                     /locus_tag="VFX38_03070"
     CDS             complement(3255..>4173)
                     /locus_tag="VFX38_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313874.1"
                     /translation="RSWGECSMSVLQLSDEVAPRLWATVRRIAMEVRVQPPHNLLLGL
                     GATFFVTRAPILTPHKKVYGYSLYLPAMALRLLSEEELAASLGHELAHLRPGMSPYVI
                     QLAANGTNGVERETERFPMAVFGIPGINTIVVVMHFLSWFAKVGFPAFELKADGIGIR
                     ASTAASAIRSLVKQAILTSSYPRTPQATRRSNARPIELVEEKNVSLDYTQKLREMLAN
                     ADPQRVRLWLQRYEEENPSPFHPTLAARAEALDVNLDTAISEVVENLKHHESSFLEEI
                     TPAEEELTGLQKTSHKKETVADIPANAIA"
BASE COUNT          772 a         1257 c         1308 g          836 t
ORIGIN      
        1 agcgcggccg gtgcgggccc cagggatagt ggatgggtcc cgcgattgca agcgcctgct
       61 cgacttcggt gtaatagcgc cacatctcgc gccagtcgag cggccagtcg gcaccgtagc
      121 cgagtgcgct gcgcgacttg aaccattccg ggcgaaagcg cagcgacacc attgcgaaat
      181 gcacggtcga gcctccgacg gccttgccac tgttgttgct gccgagcttg agcggcgctt
      241 cgccgtcgca gatgcgctcg tcggtccaga acagctttcc ctgatgggtc tcgtcggaag
      301 cgaactcctc gagcggccgc caccatgcgc cggcgtcgag acccactacc gagaagccgt
      361 actcggccag tttgcaggcg agcgtgccgc cgcccgcgcc cgtgccgacg atggcgaaat
      421 ccacttcttc gtcttcgcga tactggcgca tcggcatcca gccgccgacg cgcagcggat
      481 cgggcgcgcg accgtgggcg ccgcgcggcg cgtgcagcgc gtcatccaac acgttcgttt
      541 ttcctgcgcg cttcggcttc gcggccgggc gtcgcttcca ccccttccca gggatcgcgc
      601 cgattggcct tcatgcgaac gtagccgcgc gggctcgccg gaccgccgaa gccgatctcg
      661 ttccaggcgg tcggatgcga ataataggcg gcgagaatgt cgtgcgccag acgctcgttg
      721 aagaaggtgt ccgcaggcat gccgccccac tcgggtccgg tgagttcgcc ttgctgcatc
      781 gctttgagca gtgcgtcctg tttgctcgtg ggcagcacgt gaaactctgt ttcgtgccgt
      841 tctcgcgcag cggcatcgag cgccgcgagg ccgcgtcgcc aggcctcgcg cagcggcggc
      901 aggctcgcct gccggtaccc gtcgcgtgcg tcgcgggtca gtttccgatc gaccatcgcg
      961 gcgatcggga cgggcgtgcg cgtcggcggt tgcggcacga tgcgagcgca gattgccgtc
     1021 agcgttcgcc attcggcggt ggtgaaaaaa tggggctcgt cggcgattgc cagacgttcg
     1081 gcaatcacgc gacgcgtcgg ttcgttccac gacggcgaat cgcgcttggc gagtacatcg
     1141 tagcccgggt agcgctcagg catcgggatg atcttcgagc agacggagcg cggcaagtcc
     1201 ggcgagtgcg agccccgtga agctcggcgg cgccgggatc ggcggcccgt tgagtacgtt
     1261 ttgccgccag ttgtgccagc cgcccatgtt gcggtggacg ccccagatgt ggaagccggc
     1321 gccggcgaag ccaagcagcg cagtcaggcg cagccacaag cgcgtgaaac gacggggacg
     1381 actcgcggcg gtttcgccca acagcacggc ggcaatgggc gggaccgtta ccggcagata
     1441 catgaacgga tcgtgatagg caccgcgaaa atgcagcagc ccggcctcgc cgacggtggc
     1501 gagcaggccg aggccgctca atgccgcgag tgcgcggccg gcgggcatgc caaatacctt
     1561 cggcgtgcgc ccggattcgt tctcgcgcag ccgttccgaa tagtagccga gcaatccggc
     1621 aagagccact gccgcgggcg cgcccagcgg agaaccgtag aacaagttct gccacgagaa
     1681 tcctcccggg cgcttggcaa tgttgtaaat gtgaaatccg gtgccgacga gtccggtcaa
     1741 cgccgacgcg agatacgtca tgtcgcgaaa tttgtgtacg gcggggcgct tgtcgccagt
     1801 gccgtgcgcg cttgcgccca cagccagtgc cgagacgatc agcggtgtga acatcgcctt
     1861 gttctggaat gagccgcggt agtgttcgat cgcgctgtcc agcaaggtgg aagtcgcaat
     1921 gaggcccgct gcgcgattca gacgccgcgc tgccttgacc gtgggcggcg acggggcgcg
     1981 agcagatttt ttccgtggac gcttttcctt cccgcgcggc gcctcatcgc ggcgaagcag
     2041 ggtactgccg acgccgagcg caacgatacc gagcacgcag ttgcgtatct ggcggtcggt
     2101 aagcatgccg ttgctccttc tcgccgttct gatccgtctc catggtcgtc attgcggcaa
     2161 acatgggcgc aggcgcgacg agtacacgcg ggacagtctt caatgataac cgaagtattc
     2221 ggtagctgaa attgcaatgc cgaaactggg cgtcgaaagt tgctcgtcaa ttttgtgcgc
     2281 ttgcgcacaa ttctctttag ccgaatgctt gatcgccgcg tgcgcaactt cggacttcat
     2341 tcgatccttg ccgaaggcag tgggagctat gctgcgccga gcaaagtccg aacggattcc
     2401 atgcttcgcg gcgcaaaaac aaaagccccg ccgaagaggg cggggctttc gtgtccgatt
     2461 cggatcagat ccaaccttgg gttaccagcg ggagccgctt ctgccgccgc cgttgctccg
     2521 cggcttgtcc atggcgacgt tcaccttgag cgggcgaccg ccgaggtcct tgccattttg
     2581 ctcgagggcc ttctcggccg ccgcctgagt cgcaaaggtg atgaatccga agcccttggg
     2641 acgaccgctg tcgcggtcca tgatcagggc gagttccgaa atttcgccgt attgggaaaa
     2701 aatcccgcgc agttgcgcct cgtccaccga atacgggaaa ttaccgacgt acaacttgtt
     2761 ctgagccatt gaatctatct aaaacctctc aaaaaacgat tgaaaacaca cattgctacc
     2821 gttgcagccg gtgacatcaa atctgtcacc cggccacaaa gtagcgatct atcataacat
     2881 atcggaaaga ataaagagaa aaaacaaggt ggtaaacgaa acgccgccgc tattcgcccg
     2941 aaaagttccg ataaccttgc ctcttcagcg agggccagtg tgccgaccgt cacgtcaccg
     3001 gagagttact tgcgcccatc gtgagtggcg aggtcgcggt cgagttcggg gagtgccagt
     3061 cgcggaagat gccccgttcg cgacgcatcg cggcgacatc gccgaggccc gatgcggcgg
     3121 tgaggatggt tcccttggtg cgattgcctt caaggccgac agcgccagcc aggttggaac
     3181 gtttttggtt tccgaccggg aagtggtttt gccgaacatc gaatgtgtcc ctgatgtgtg
     3241 tcccgggttg aatatcacgc gattgcgttc gccgggatat ctgcgaccgt ttcctttttg
     3301 tgcgacgttt tctgaaggcc tgtgagttct tcctctgcgg gagtgatttc ttccaggaat
     3361 gatgattcgt ggtgcttcag attttccacc acttcggaaa tggcggtatc gagattgaca
     3421 tcaagcgctt cagccctcgc cgcaagcgtc gggtgaaacg gtgaggggtt ctcttcttcg
     3481 taacgctgca gccaaagtcg cacgcgttga ggatctgcat ttgcgagcat ttctcgcaac
     3541 ttctgcgtgt aatctaagct gacgtttttt tcttccacca gttcgattgg ccgcgcgttc
     3601 gatcttcgtg tcgcttgggg cgtgcgtgga taagacgatg tcaggatggc ctgtttcacg
     3661 agacttcgta tcgctgacgc cgccgtgctc gcgcggatgc caattccatc cgctttcagc
     3721 tcgaacgccg gaaatcccac tttcgcgaac caggatagaa aatgcattac gaccactatg
     3781 gtattgatcc caggaatccc gaaaactgcc attgggaacc gctcggtttc tcgttcgacg
     3841 ccattcgttc cgttcgcggc gagttgaatg acatagggcg acatgcccgg acgaaggtga
     3901 gccagctcat gccctagtga ggcggcaagc tcttcctcgg atagcagacg tagcgccatc
     3961 gcaggtagat atagcgaata tccgtaaacc tttttgtgcg gtgtcaggat gggggctcgt
     4021 gtgacaaaga aggtggctcc gaggccgagg agcaggttat gcggcggttg gactctcacc
     4081 tccatcgcga tacgtctcac ggtcgcccaa agtcgcgggg ccacctcatc gctcaactgc
     4141 aggaccgaca tcgagcactc tccccagctc ctg
//
LOCUS       DASUJG010000146         2252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_653935, whole genome shotgun sequence.
ACCESSION   DASUJG010000146 DASUJG010000000
VERSION     DASUJG010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653935"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /gene="fghA"
                     /locus_tag="VFX38_03075"
     CDS             <1..684
                     /gene="fghA"
                     /locus_tag="VFX38_03075"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEX5313875.1"
                     /translation="KAGAERYAAELGLLLVAPDTSPRGLGLAGETASYDLGEGASFYV
                     NATEPPWSAHYRMYDYLRDELPALIAAHFPVLADRAAIAGHSMGGHGALVLALRNPDR
                     YRSVSAFAPIAAPSLCPWGRKAFTAYLGADESRWRDYDAHELIRKTTARLLLRIDQGS
                     EDEFLHSGQLLPEKLLEAAAAADYPLDYRLREGYDHSYWFVASFIGEHLAWHAAALRS
                     DSKAKKPVR"
     gene            708..>2252
                     /locus_tag="VFX38_03080"
     CDS             708..>2252
                     /locus_tag="VFX38_03080"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HEX5313876.1"
                     /translation="MRRLAFLLVLLAPGIALAASPTAAEILAQSKAASGGALWDSVTS
                     LQIKGKMMKGGLEGPADKSVDMTTGHSVSHFQIGGYRGGEGYDGTTGWMQAPSGEISA
                     ENSDAQKQAEVTQAYILARGYWFPDRWPAAINLLGTRSEGEDRFEVLQITPQGGDPFE
                     LWINAETHLVDRTVVTTPAGQETRSYNEYLSVNGVQIPFHQWLRDDQNHYTIFQYDAA
                     SVNLPFNAKDFAMPQQNLNDYAFLGGGNQATMPFQLIHNRVYLGATVGSQPLQLQLDS
                     AAVNVLTGEAAGRLGVKSAGSFPVSGPQGQPLDVSVAKIDSMTLGGDVTLSNQLFRVV
                     QLPGISDADGTRFDGFVGFELLKRFVVQVNYAYRKVTLILPGSFNATDAGTPVPFTLT
                     NGRIPTVSATIDGISGPFAIDTGSTGMVNLEPAFVKTHDLLARYETTPQTTIGWGVGG
                     AETGRAARAGNFAVGGVAMGAPVILLDDANVPADVGQAFAGVIGGALLRRFTLTFDYA
                     NQRIYLK"
BASE COUNT          406 a          761 c          697 g          388 t
ORIGIN      
        1 aaggcgggag cggagcgcta cgccgccgaa ctcggattgc tgctcgtcgc gcccgacacc
       61 agtccgcgcg gactcggact tgcgggggaa accgcaagct acgatctcgg cgaaggcgcg
      121 tccttttacg tgaacgcgac cgaaccgccg tggtcggcgc attaccgcat gtacgattac
      181 ctgcgcgatg aattgcccgc gctgatcgcg gcgcatttcc cggtgcttgc cgatcgtgcc
      241 gccatcgccg gtcattcgat gggcgggcac ggcgctctcg tcctcgcgct ccgcaatccc
      301 gatcgctatc gttcggtctc cgcgtttgcg ccgatcgccg cgccctcgct ctgcccctgg
      361 gggcgcaagg ccttcacggc ctatctcgga gcggacgaat cgcgctggcg cgactacgat
      421 gcgcacgaac tcatcaggaa aacgaccgcg cgtctgctgc tgcgcatcga tcagggcagc
      481 gaagacgaat tcctccattc cgggcaactt ctgccggaga aactgctgga agcggccgcc
      541 gcagcggact atcccctgga ctatcggctg cgcgagggct acgaccacag ctactggttc
      601 gtcgcaagct tcatcggcga gcatcttgcc tggcacgcgg cggcgctgcg ctccgactcg
      661 aaggcgaaaa aaccggtacg ataaccgttt tccccagctc gaaccccatg cgccgactgg
      721 cctttcttct cgtgcttctt gctcccggca tcgccctcgc ggcctcgccc acggcggccg
      781 aaatcctcgc ccagtcgaag gcggccagcg gaggcgccct ctgggacagc gtcacctcgc
      841 ttcagatcaa gggcaagatg atgaagggcg ggctcgaagg ccccgccgac aagtcggtgg
      901 acatgacgac gggacattcg gtgagccatt tccagatcgg cgggtatcgc ggcggggagg
      961 gctacgacgg aaccacgggc tggatgcagg ctccgagcgg agagatctcc gccgagaatt
     1021 ccgacgcgca gaagcaggcc gaggtaaccc aggcgtacat cctcgcccgc ggctactggt
     1081 ttcccgatcg ctggccggcc gcgatcaatc ttctcggcac gcgcagcgaa ggagaggacc
     1141 gtttcgaggt gctccagatc accccgcagg gcggcgatcc cttcgaactg tggatcaatg
     1201 ccgagacgca tctggtggac cgtaccgtcg tcaccactcc cgcgggacag gaaacccgct
     1261 cctacaacga atatctctcg gtcaacggcg tgcagattcc gtttcaccag tggctccgcg
     1321 acgaccagaa ccactacacc atctttcagt acgatgcggc ctcggtgaac ctgccgttca
     1381 acgcgaagga tttcgcgatg ccgcagcaga atctcaacga ctatgcgttt ctcggcggcg
     1441 gcaatcaggc gacgatgccc ttccagctca tccacaatcg cgtctatctc ggcgcgaccg
     1501 tcggcagcca gccgctccaa ctgcaactcg acagcgccgc cgtgaacgtt ctcaccggcg
     1561 aggcggcggg ccgtctcggc gtgaaaagcg ccggcagttt tccggtgtcc gggccgcagg
     1621 gtcagccgct cgacgtgagt gtcgcgaaaa tcgacagcat gacgctcggg ggcgatgtga
     1681 cgctcagcaa tcagctcttc cgggtcgtac agcttcccgg catcagcgat gccgacggca
     1741 cgcgcttcga tggtttcgtc ggcttcgaac tgctgaagcg cttcgtggta caggtcaact
     1801 acgcctaccg caaggtgacg ctcatccttc cgggaagctt caacgcgacc gatgcgggca
     1861 cgccggtgcc gttcacgctc accaacgggc gcatcccgac ggtgagcgcg acgatcgacg
     1921 gaatcagcgg gcctttcgcg atcgataccg gaagcaccgg aatggtcaat ctcgaacccg
     1981 ccttcgtcaa gacgcacgac cttctcgcgc gctacgagac gacgccgcag acgacgatcg
     2041 gctggggagt cggcggcgcg gagacgggcc gggcggcgcg cgccggaaac tttgcggtgg
     2101 gcggagtcgc gatgggcgcc ccggtaattc tcctcgacga tgccaacgtt cccgccgacg
     2161 tcggccaggc cttcgcgggc gtcatcggcg gcgcacttct tcgccgcttc acgctcacct
     2221 tcgattatgc gaatcagcgc atctatttga ag
//
LOCUS       DASUJG010000147         2783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_531991, whole genome shotgun sequence.
ACCESSION   DASUJG010000147 DASUJG010000000
VERSION     DASUJG010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531991"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2230)
                     /locus_tag="VFX38_03085"
     CDS             complement(<1..2230)
                     /locus_tag="VFX38_03085"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX5313877.1"
                     /translation="MKSLEQRHDSTPLYGGSAPYVETLYERWLADTRSVAAEWREYFE
                     RFGERGDRARAPVEAELAARAHARRGAASADGDGGAGQRLLDAWRLLGHLTADIDPLK
                     LREIPRIDELEPGAQGLSGGDLATAVATDWAGRRDRRPLGELLEAARKVYGGHTAIEF
                     THVSDADEWHWLAEHIEGGEGSAALTDEERRRVLAELTAAEGLEKYLHRRFVGQKRFS
                     LEGGETLIPMLNDLFRRAGGDGVKEIVIGMAHRGRLNVLVNILGKPPQDIFSEFEGSV
                     AESERAASGDVKYHLGQSDDVETAGGPMHLVLAFNPSHLEAVDPVVEGSVRARQDRAG
                     DEAGAQVLPVLIHGDASIAGQGVVMETLQMSQTRGFGTGGTVHIVLNNQIGFTISDLA
                     DARSSCYCTDIAKMLEAPVLHVNGDDPEAALAALRLAFAYRQRFHKDAFVDLVCYRRL
                     GHNEADEPAVTQPRMYAAIRSHSTTRELYARKLEESKVIADGAADELADRYNDGLDAG
                     RVIEEAALKRVEGEFVQTSWHEFEGQSWDQNVDTGIAPGRLRELGDKLFSVPEGFTLH
                     GRTERVVSERRKMLAGEVPVDWGCAENLAYATLVTAGHPVRLTGQDTVRGTFFHRHAS
                     YFDSETGDAWTPLNHLAEDQAPFMVYDSLLSEAAVVGFEYGYATARPRALVLWEAQYG
                     DFANNAQVMIDQFISSGEAKWGRLCGLVLLLPHGYEGAGPEHSSARLERYLQLCAGSN
                     MQV"
     gene            2501..>2783
                     /locus_tag="VFX38_03090"
     CDS             2501..>2783
                     /locus_tag="VFX38_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012480865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HEX5313878.1"
                     /translation="MIDNRRYPLLARIESPADLRALPEERLGAVARELRAYLIETVSG
                     IGGHFAAGLGTVELTVALHYVYDTPTDRLVWDVGHQAYPHKVLTGRRDRL"
BASE COUNT          446 a          968 c          893 g          476 t
ORIGIN      
        1 agacctgcat gttgcttccg gcacaaagct ggaggtagcg ttcgagccgc gccgaggaat
       61 gctcgggccc ggcgccctcg tagccgtgcg gaagcagcag caccagtccg cagagccgcc
      121 cccacttcgc ctcgcccgaa ctgatgaact gatcgatcat cacctgcgcg ttgttcgcga
      181 agtcgccgta ttgcgcctcc cacaatacca gcgctctcgg ccgcgccgtc gcgtagccgt
      241 attcgaatcc gacgaccgcg gcttcggaaa ggagcgagtc gtacaccatg aacggcgcct
      301 gatcctccgc gagatgattg agcggcgtcc aggcgtcgcc ggtttcgctg tcgaaatagc
      361 tcgcgtggcg gtggaaaaag gtaccgcgca ccgtgtcctg tccggtgagg cgcaccggat
      421 ggcccgcggt gacgagcgtc gcgtaggcga gattctccgc gcagccccag tccaccggga
      481 cttcacccgc gagcatcttg cgccgctcgc tcacgacgcg ttcggtgcgc ccgtgcaagg
      541 taaatccttc gggcacggag aagagtttgt cgccgagttc ccgcaggcgt ccgggcgcga
      601 tgccggtgtc cacgttctga tcccagctct ggccctcgaa ctcgtgccag ctcgtctgca
      661 cgaattctcc ttcgacgcgc ttgagcgccg cctcttcgat tacgcgaccg gcatcgagac
      721 cgtcgttgta gcgatcggcg agctcatcgg ccgcaccgtc ggcgatgact ttcgattctt
      781 cgagcttgcg cgcgtacaac tcgcgggtgg tggaatgact gcggatcgcc gcgtacatcc
      841 gcggctgggt gaccgccggc tcgtcggcct cgttgtggcc gagccggcga tagcagacca
      901 gatccacgaa ggcgtccttg tgaaagcgct ggcgataggc gaaggcgaga cgcagggccg
      961 cgagcgccgc ctcgggatcg tcgccgttga catgcagcac cggcgcctcg agcatcttcg
     1021 cgatgtcggt gcaatagcag ctcgagcgcg catcggcgag atcgctgatg gtgaagccga
     1081 tctggttgtt gagcacgatg tgcacggtgc cgccggttcc gaatccccgg gtctgcgaca
     1141 tctggagcgt ttccatcacc acgccctggc cggcgatcga ggcgtccccg tggatcagca
     1201 ccggcagcac ctgcgcgcct gcctcgtctc ccgcgcggtc ctgacgtgcg cgcaccgagc
     1261 cctcgaccac cgggtccacc gcttcgagat gcgagggatt gaaggcgagc accagatgca
     1321 tcgggccccc ggccgtttcg acatcgtccg actggccgag gtgatatttg acatcgccgc
     1381 tcgcggcccg ctccgattcg gcgacactgc cttcaaattc ggagaaaatg tcttgcggcg
     1441 gcttgccgag gatgttgacg agcacgttga gccggccgcg gtgcgccatg ccgatcacga
     1501 tctccttcac gccgtcgccg ccggcgcgac gaaagagatc gttcagcatc ggaatcaaag
     1561 tctcgccgcc ttcgagcgaa aaacgcttct ggccgacgaa gcgccgatgg agatattttt
     1621 cgagcccttc ggccgccgtc aattcggcga gcacgcgccg gcgctcctcg tccgtcaggg
     1681 cggcgctgcc ctcgccgccc tcgatgtgtt cggcgagcca gtgccattcg tccgcatccg
     1741 atacatgcgt gaattcgatc gcggtgtggc cgccatagac tttgcgcgcc gcctcgagca
     1801 attcgccgag cgggcgccgg tcgcggcgtc cggcccaatc ggtcgcgacc gccgtcgcaa
     1861 gatcgccgcc gctcaaaccc tgagcgcccg gttcgagttc gtcgatgcgc ggaatctccc
     1921 gcagcttcag ggggtcgatg tcggccgtaa gatgcccgag aaggcgccag gcgtcgagca
     1981 ggcgctggcc cgccccgcca tcgccgtccg cgctcgccgc gccgcgccgc gcgtgggcgc
     2041 gcgccgccaa ctcggcctct accggcgcgc gcgcacggtc gccgcgttcg ccgaagcgct
     2101 cgaaatactc gcgccactcg gccgcaaccg agcgcgtatc ggcgagccag cgctcgtaga
     2161 gcgtctcgac atagggagcg ctgccgccgt agagcggtgt cgagtcgtgt cgctgctcaa
     2221 gacttttcat tgccgacttc gtcgattacc ctgcgctcgc gtccgcgaaa ccgttccgag
     2281 ccgccgtgtg cggcgagcga actgccacgt cgcaaccgaa ggctcgggga tcgggaaaca
     2341 acggcgatct gcagcccgcg tccgccattt tagccgatcg ccgcgacgcc gcaggccgtg
     2401 gaacggcgcg atccggcgcc acgcggcggc gcgcagcgct tgaatcggtc tgccaagacc
     2461 gcgataataa gagacctccc cgtcccccca ggccgcgtcg ttgatcgata accgacgata
     2521 tcccctgctc gcaagaatcg aatcgccggc ggatctgcgc gcgctgcccg aagagcgtct
     2581 gggggcggtc gcccgcgaac ttcgcgccta tctgatcgaa accgtgagcg gcatcggcgg
     2641 gcacttcgcg gccggtctcg gcacggtgga actcaccgtc gctctgcatt atgtctatga
     2701 cacgcccacc gaccggctcg tctgggacgt cggccatcag gcctatccgc acaaggtgct
     2761 gaccggccgg cgcgaccggc tcg
//
LOCUS       DASUJG010000148         3182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1510589, whole genome shotgun sequence.
ACCESSION   DASUJG010000148 DASUJG010000000
VERSION     DASUJG010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510589"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(231..2297)
                     /locus_tag="VFX38_03095"
     CDS             complement(231..2297)
                     /locus_tag="VFX38_03095"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX5313879.1"
                     /translation="MHYNRSAMRLFFIPAAAVSRSVKPGLAAALLLAAAPVFAAAPAP
                     ASGIAAVRAAFVRAYASAQLGGNAWRERAAGLENYPLYPYLEATALEHDLATATPTEV
                     EAYLERYPGLIPAQDLRRDFLRELASRKDWHDFLVIYRPGLGNTLACDALRARLAGGG
                     ELDFEHDLSALWAEPSLPGACDPVLQWAQEHGLLTRERLFARIDAAAQAGYGGTVASL
                     ARWLPAADARVVRRLALALRDPAEAVHVAANWPDDPRDREAAMYAITGFAREDSARAL
                     AEWPALAEHFHFDSATTDHILAALALYRASDFGDDALALLTALPPAAQTDATREWRVR
                     IALAHEDWPAALAALDALTPAQQDDLEWRYFRARALAATGHETEADALYAALARETDY
                     FGFLAADRIGTPYAICPRTPDEETTDAQRLLANPGLERAFELFAVGMLRDARREWDAA
                     FADADDETVDAAGFLAARRGWYSRPIVAFARTRSWKYYDERFPLAWQSDVSAAAKASG
                     LDPAWVNGLIRAESAWMPDAGSDAGAQGLMQLMPSTARMVARQQQIPYDDDLYDPAVN
                     IALGTRYLQDMALRYDDAAWLATAAYNAGPGRVDRWLAARGSLPPDLFVATIPWQETR
                     EYVARVLAYSVIYDWRLNGNAVAISRRMPPYGSFDPPPSATTPRKAVACPLPAATKSG
                     AATKPP"
     gene            complement(2307..>3182)
                     /locus_tag="VFX38_03100"
     CDS             complement(2307..>3182)
                     /locus_tag="VFX38_03100"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5313880.1"
                     /translation="MLRVLAGAILISFAGILVPLSGVASAPAAFYRLAIGGVALAALA
                     LLRRERLEVEGRLAAFVLLAGFAFACDLVTWHLSIYRVGPGIATLIANFQVVLMALAG
                     WLFWRERLGPRGLLAIAIAVAGLVLLLVAAPQSGNGRFLAGVGYGLGAALAYTLYLLF
                     LRQAGASASRAAPAFFIALVTLLAAAVVACWLAIRGESFVVPYGMAWLWLALYGLGCQ
                     AGGWFLISTGLPRVTAALAGLLLLLQPTLTFAWDRLIFNRRFTWLEVLGAALALLGLY
                     LGMRWRGARKLRAAR"
BASE COUNT          609 a         1025 c         1136 g          412 t
ORIGIN      
        1 gcggtatcgc ttcaccttcg ccgctcacgc ttcgcaagcg cactgaacga cgccgcgatt
       61 gcagcactcc gcacagtaaa acacggacat ccccctcgat cccccttccc gagggaaggg
      121 cgaagcatga caggagccca agaccaggaa tccattgccg gaacgatgga ttgcccgcaa
      181 cgtttcgctt atgcaggtct gcgcctgttt tccaatgcgg caaaggagta tcagggtggc
      241 ttggtggccg cgcccgactt cgtggcggcg ggcagcgggc aggcgacggc cttgcgcggc
      301 gtcgtcgccg aaggtggcgg atcgaagctt ccgtagggcg gcatgcgccg cgagattgcc
      361 accgcattgc cgttgagacg ccaatcgtag atcacgctgt aggcgagcac gcgcgcgacg
      421 tattcgcgtg tctcctgcca gggaatcgtc gcgacgaaga gatcgggcgg aagggaaccg
      481 cgagccgcaa gccagcggtc cacgcgtccg gggccggcgt tgtaagccgc ggtcgcaagc
      541 caggccgcat cgtcgtaacg caacgccatg tcctgcagat accgagtgcc gagcgcaatg
      601 ttgacggccg gatcgtagag atcgtcgtcg tagggaatct gctgctgtcg ggcgaccatt
      661 ctggcagtgg acggcatgag ctgcatcagg ccctgcgctc ccgcatcgga acccgcgtcc
      721 ggcatccacg cgctttcggc gcggatgaga ccgttcaccc aggcgggatc aagaccgctt
      781 gccttcgccg ctgcggaaac gtcgctctgc caggcgagcg ggaagcgctc gtcgtaatac
      841 ttccaggaac gggtgcgggc gaaggcgacg atgggacggc tgtaccagcc tcggcgggcc
      901 gcgagaaagc cggcggcatc gacggtctca tcgtcggcgt cggcgaacgc ggcgtcccat
      961 tcgcggcgcg catcgcggag catcccgacg gcgaacagtt cgaaggcccg ctcgagtccg
     1021 ggattggcga gcaggcgttg cgcatccgtc gtttcctcat cgggcgtgcg cggacaaatg
     1081 gcataggggg tgccgatccg atcggccgcg aggaaaccga agtaatcggt ttcgcgcgcg
     1141 agcgccgcat acagcgcatc ggcttcggtc tcgtggccgg tcgcggcgag agcgcgcgcg
     1201 cggaaatagc gccattcgag atcgtcctgt tgcgcgggcg tgagtgcatc gagcgcggcg
     1261 agcgccgccg gccaatcctc gtgggcgagc gcgatccgca cccgccactc gcgggtcgca
     1321 tcggtctggg cggccggcgg cagcgccgtg agaagggcga gcgcgtcgtc gccgaaatcg
     1381 ctcgcgcgat agagcgcgag cgcggcgaga atgtgatcgg tagtcgcgga atcgaaatga
     1441 aagtgctcgg cgagcgccgg ccattcggcg agcgctctcg cgctgtcctc gcgcgcgaaa
     1501 ccggtgatcg catacatggc ggcttcgcga tcgcgcggat cgtccggcca gttcgcggcg
     1561 acatgcaccg cctccgccgg atcgcgcaac gcgagcgcga ggcgccgcac gacgcgggca
     1621 tcggcggcgg gaagccagcg cgcgagcgag gcaacggtgc cgccatagcc cgcctgcgcg
     1681 gcggcatcga tgcgtgcgaa caatcgctcg cgcgtaagca aaccgtgttc ctgcgcccat
     1741 tgcaataccg ggtcgcaagc gcccggcagc gacggctcgg cccagagggc ggaaagatca
     1801 tgctcgaagt ccagctcccc gccgcctgcg agacgtgccc gcaaggcatc acaggcgagg
     1861 gtgttgccga gacccgggcg atagatcacg agaaagtcgt gccagtcctt gcggctcgcg
     1921 agttcgcgca ggaaatcgcg gcgcaggtcc tgcgcgggaa tgagaccggg atagcgctcg
     1981 agatacgctt cgacttcggt cggggtcgcc gtggctagat cgtgctcgag cgccgtcgcc
     2041 tcgagatagg gatagagcgg ataattctcg agcccggcgg cgcgctcgcg ccacgcattg
     2101 ccgccgagtt gcgccgaggc gtaggcgcgg acaaaggccg cccgcaccgc cgcgattccg
     2161 ctcgccggcg cgggagcagc ggcaaagacg ggggcggctg cgagaagcaa cgcggcggcg
     2221 aggccgggtt tcaccgaacg cgaaacggcc gcggccggga tgaagaacaa gcgcatcgcc
     2281 gatcgattgt agtgcatcgg cgaagctcag cgggcggcgc gaagtttgcg cgccccgcgc
     2341 cagcgcatgc cgagatacag tccgaggagc gcgagtgcgg cgccgagcac ttcgagccag
     2401 gtgaagcggc gattgaaaat caggcggtcc caggcgaagg tgagcgtcgg ctgcagcagg
     2461 agcagcaaac cggcgagcgc cgccgtcacc cgcggcaggc cggtcgaaat gagaaaccag
     2521 ccgccggcct gacagccgag accgtagagc gcgagccaca gccaagccat cccgtaggga
     2581 acgacgaaac tttcgccgcg aatcgcgagc cagcaggcga ccacggcggc ggcgagaagc
     2641 gtgaccagcg cgatgaaaaa cgcgggcgcg gcgcggctcg cggatgctcc cgcctgccgc
     2701 agaaagagaa gatacaaggt atacgcgagg gccgcgccga gtccgtagcc gacgccggcg
     2761 agaaaccgcc cgttgcccga ttggggcgca gcgacgagca gaagcacgag accggcgacc
     2821 gcaatcgcga tggcaagcag gccgcgcggg ccgaggcgtt cgcgccagaa caaccagccg
     2881 gcgagcgcca tcaatacgac ctggaaattc gcgatcagag tcgcgattcc cggtccgacc
     2941 cgatagatgc tcaggtgcca ggtgacgaga tcgcaggcga aggcgaagcc cgcaagcagc
     3001 acgaacgccg cgagacggcc ctcgacttca aggcgttcgc ggcgcagaag ggcgagcgcg
     3061 gcgagcgcga cgccgccgat tgcaagccga taaaacgccg cgggcgccga ggcgacgccc
     3121 gaaagcggca cgaggatgcc cgcgaagctg atgagaatcg cgcccgcgag tacccgcagc
     3181 at
//
LOCUS       DASUJG010000149         8436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_654116, whole genome shotgun sequence.
ACCESSION   DASUJG010000149 DASUJG010000000
VERSION     DASUJG010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8436
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654116"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /gene="eno"
                     /locus_tag="VFX38_03105"
     CDS             complement(<1..233)
                     /gene="eno"
                     /locus_tag="VFX38_03105"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809626.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX5313881.1"
                     /translation="MNEEFEIVELLPREILDSRGNPTVEVECVLACGVRARAAVPSGA
                     STGEREAAELRDEDPKRYGGKGVLKACHHIEHDL"
     gene            complement(248..931)
                     /gene="pgl"
                     /locus_tag="VFX38_03110"
     CDS             complement(248..931)
                     /gene="pgl"
                     /locus_tag="VFX38_03110"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX5313882.1"
                     /translation="MKLEVFRDADGACERGAEILADQIHAAMRERGRALVALSGGRTP
                     WAMLEKLAAKELPWDRILFFQVDERDCPAEDDARNLKHLHACLPVEAQIEAMPVESGE
                     AGAGRYASRLASSAGNPPVLDVVHLGLGPDGHTASLVPGDAVLEVRDREVAWTLSAYQ
                     GRRRMTVTYPLLDRARFVFWLVAGEDKREALAKLLDGDSSIPAGRVRNAHSLVLAEAT
                     AAGEMSSDR"
     gene            complement(987..1592)
                     /locus_tag="VFX38_03115"
     CDS             complement(987..1592)
                     /locus_tag="VFX38_03115"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011935172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX5313883.1"
                     /translation="MKRRLWLVRHGETDWSAAGKHTGRTDVPLNERGREQARALGRGL
                     AALGVKFAASYVSPLGRARETAALAGFPDAEALPDLREWDYGEFEGRKTADIRRETND
                     PEWLIWTADIREGESPAAVGARCDRVGDRLAAGAGDVILFAHGHFLRMFAARWLALAA
                     VAGQHLALDPGTVSILGYEHEYRVIARWNAPLAADGNGSKT"
     gene            complement(1589..2983)
                     /gene="zwf"
                     /locus_tag="VFX38_03120"
     CDS             complement(1589..2983)
                     /gene="zwf"
                     /locus_tag="VFX38_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX5313884.1"
                     /translation="MSAETADGSDALVLFGATGDLAYKKIFPSLERLEIAGELEMPVI
                     GVASSDWTADQLRERARKSIAENGENKDKKAVERLLQRLDYIAGDYRDDKTLGELTKR
                     LDGAAHPLFYLAIPPSLFATVVEGLQKAKLSKKGSVVVEKPFGRDLDSALALDRTLSE
                     VFPEERIYRIDHYLGKEPVQNLLYFRFANTFLEPFWNRTYIESIQITMAESFGVEDRG
                     AFYDEVGAIRDVVQNHMLQELSILAMEPPAASDAHAMRDEKAKLMASIRPLSAQNLVR
                     GQYNGYRKIEGVKSNSTRETFAALRLEIDNWRWAGVPIFIRAGKMLPVDAQEVVVRLH
                     RPPLDIFSEPLASNSNHIRFRLGPDCVEIAIGARVKQPGEIMAGRRVELEVASDPSVD
                     EPPYERLIGDALKGDQELFARSDAVLAAWRVVDPALAVSTPVYAYEPHSWGPQEAQAM
                     TAAFGGWHDPAGSK"
     gene            complement(2988..4001)
                     /gene="gnd"
                     /locus_tag="VFX38_03125"
     CDS             complement(2988..4001)
                     /gene="gnd"
                     /locus_tag="VFX38_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEX5313885.1"
                     /translation="MQFGMIGLGRMGANMVKRLHNAGHQAVVYDVSADAVKRLAAEGA
                     TASDSLEDFVGKLKSPRVVWMMVPTAFVEKTIEALRPLLAEGDILVDGGNSHYKDAIR
                     QAKTLAGDGIHLLDAGVSGGVWGYERGYCLMIGGNADACAHLDPIFRALAPGVEAAEP
                     TPSRGKRSGSAPQGYLRCGEAGAGHFVKMVHNGIEYGIMAAFAEGFNVLRHADAGTRK
                     YDEDAETSPLDDPDNYRYQLDIPEIAELWRRGSVIGSWLLDLTADALARDPELERFAG
                     HVSDSGEGRWTIQAAIETGTPVPVLALALFERFTSRGEAGFADKLLSAMRSEFGGHDE
                     KKD"
     gene            complement(4062..6680)
                     /locus_tag="VFX38_03130"
     CDS             complement(4062..6680)
                     /locus_tag="VFX38_03130"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HEX5313886.1"
                     /translation="MNALKELHKLGESIWMDNIRRGMLKSGQLRHYIEEFALTGLTSN
                     PTIFEHAIGGGEEYDEGIRRHLDDEHPDAAAIFFELAIEDLQQAADLFLPFWAKSDGR
                     DGYVSLELAPGLADDTEGSIRQARDLAGRAQRKNLFIKVPGTEAGAGAIEQLIYEGIP
                     VNVTLLFSPDQYRRSAEAYLKGIERRIAEGKDPHVPSVASLFVSRWDKASVDRLPEKL
                     QNKLGVAIGKETYRVHCELYASDRWKKLAGKGASPQRLLWASTGTKDPSLSDDYYVYE
                     LVAPDTINTIPEATLFSVAKDGRVGKPLPADGGDAGETLKAINEAGVDTDKLARDLQE
                     DGKKKFDESYAKLLAQIEKKLAELREASEEDRESLGKFEAGIRRQIGEIAKDNVVERI
                     WAHDHTVWKPDPDEIDNRLGWLGSPQEMMEEVPRLKEFVKGVRDAGMTHVLWSGMGGS
                     SLFPQVMQQSFGDAGGLEMIVLDTSNPVTVREAADSLPLEKTLFVFASKSGGTLETRC
                     HLDYFWSRMNDPAHYAVITDKGSDLGKIAAERKFRKTFFNNPNLGGRYSALSHFGLVA
                     AALLGVDVEELLGRAGQMIAASMPDTPAERNPGVRLGAAIAFASQQGCDKLTLVIPPE
                     IASFGSWLEQLIAESTGKDGVGILPVAGETVGKPGVYGDDRFFVCLGDQSDLGLVADS
                     HPVIRLPYVDRYSIGAECFRWEFAIAIAGHVLGINAFDQPNVEAAKKAASKVLAEGVP
                     ELPQSAASDALAAIKSGDYLAIQAYVATDSKHFAELQTARLALRDRLRVATTLGLGPR
                     YLHSTGQLHKGGHANGVFVQIVDDTAPDLEIPGRPYGFAQLFEAQAAGDFLALKEHDR
                     RVFRVALHDFLDTANA"
     gene            complement(6768..>8436)
                     /gene="ppsA"
                     /locus_tag="VFX38_03135"
     CDS             complement(6768..>8436)
                     /gene="ppsA"
                     /locus_tag="VFX38_03135"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEX5313887.1"
                     /translation="VLRRRLGSKHLTMIYADAGDPARVKNVETPEEKRRRFSLSDEDV
                     LLLADYAIKIERHYSERAGVPRPMDMEWAKDGKDGLIYMLQARPETVASQRKGGILED
                     YVIRGSGEVIVTGRAVGSKIASGKVRVARDLSALHEFRPGEVLVSETTTPDWEPVMKN
                     AAAIVTNRGGRTCHAAIVARELGIPAIVGTSEATRLIEDGAIVTVSCAEGDTGKVYDG
                     EIPFEIKRTDLSALPRPATRIMVNLGNPALAFKTSFLPNDGVGLARLEFIINESIKAH
                     PLALLHPERVKDPAAVREIGRLIAGYANGREFFIERLSESVATIAAAFYPRPVVVRLS
                     DFKSNEYAALVGGADFEPKEENPMLGFRGASRYTHPAYAEAFELECAAMKRVRETMGF
                     GNVILMIPFVRRVAEADGVIALMEKFGLKRGAKELKIYAMCEIPNNVMQIDEFATRFD
                     GFSIGSNDLTQLTLGVDRDSEIVAAAFDERDPGVRRMIRLAVEGCQRNGIHSGLCGQA
                     PSDYPEMAEFLVELGIESMSLNPDTVLATTERILEIEKRLGRPPRAH"
BASE COUNT         1424 a         2675 c         2746 g         1591 t
ORIGIN      
        1 aggtcgtgct cgatgtgatg gcaggctttc agcacgccct tgccgccgta gcgttttgga
       61 tcttcgtcgc gaagctccgc cgcctcgcgc tcgccggtgg acgctcccga gggcaccgcc
      121 gcgcgtgcgc gcacgccgca ggcgagcacg cattcgactt cgaccgtggg attgccgcgc
      181 gaatcgagaa tttcccgcgg cagcagttcg acgatctcga attcttcgtt cattttcggg
      241 gctccggtca gcggtcggaa gacatctcgc cggcggcggt ggcctcggcg agaacgaggc
      301 tgtgcgcatt gcgcacgcgc cccgcgggaa tggaagagtc gccgtcgaga agtttcgcga
      361 gcgcctcgcg cttgtcctcg ccggcgacga gccagaagac gaagcgggcg cgatcgagaa
      421 ggggataggt gacggtcatg cgccgccggc cctgataagc ggacaaagtc caggccactt
      481 cgcgatcccg cacttcgagc acggcatcgc ccggcaccag cgaggcggtg tgaccgtcgg
      541 gaccgaggcc aaggtgaacg acgtcgagca ccggcggatt gccggcgctc gacgcgaggc
      601 gcgaagcgta ccgcccggcg cctgcctctc ccgattcgac gggcatggcc tcgatctgcg
      661 cttccactgg aagacaggcg tgcaagtgct tgagatttct tgcgtcatct tcggcgggac
      721 aatcgcgttc gtccacctga aagaagagaa ttcgatccca gggcaattcc ttcgcggcca
      781 atttttcgag catcgcccag ggagtccgcc cgccgctcag ggcaacgaga gcccgaccgc
      841 gttcgcgcat cgccgcatga atttggtcgg cgagaatttc cgcgccgcgc tcgcaggcgc
      901 catcggcgtc gcgaaacacc tcaagcttca caaacctcgt ctctcgcagg ttgggttgag
      961 tcttcgaagc ccaacaacag cagaactcaa gttttgctcc cgtttccgtc cgccgcaagc
     1021 ggtgcattcc aacgcgcgat gacgcgatat tcatgctcgt aaccgagaat ggacacggtg
     1081 ccggggtcga gcgcgagatg ttgaccggcg acggcggcga gtgccagcca gcgcgccgcg
     1141 aacatccgca ggaaatgccc gtgcgcgaac aggatgacat cgccggcgcc cgcggcgagc
     1201 cggtcgccga cgcggtcgca gcgtgccccc accgcggccg gcgattcgcc ttcgcgaatg
     1261 tcggcggtcc agatcaacca ttcgggatcg ttcgtctcgc ggcggatgtc cgcggtcttg
     1321 cggccctcga attcgccgta gtcccattcg cgaagatccg gcaacgcttc ggcgtcggga
     1381 aaacccgcga gcgccgcagt ttcgcgcgcc cgtccgagag gggagacata actcgccgcg
     1441 aacttcacgc cgagtgccgc aagcccgcgg ccgagcgcgc gcgcctgctc gcgtccgcgt
     1501 tcgttcagag ggacgtcggt tcgcccggtg tgcttgcctg ccgcgctcca atcggtttcg
     1561 ccgtggcgca ccagccacag gcggcgtttc atttcgagcc tgcgggatcg tgccagccgc
     1621 cgaaggcagc cgtcatcgcc tgcgcttcct gcgggcccca gctatggggc tcgtacgcgt
     1681 agacgggggt ggagaccgca agcgccggat ccaccacgcg ccaggcggcg agcaccgcat
     1741 cgctgcgggc gaagagttcc tgatcgccct tgagcgcatc gccgatgagg cgttcgtagg
     1801 gcggctcgtc caccgaagga tcgctcgcga cctcgagttc gacgcgcctg ccggccatga
     1861 tctcgcccgg ctgcttcacg cgcgccccga tcgcgatctc gacgcagtcc ggcccgagcc
     1921 ggaagcggat gtgattgctg ttggaggcga gcggttcgct gaagatgtcg agcggcgggc
     1981 ggtgcagacg caccacgact tcctgcgcgt ccaccgggag catcttgccg gcgcggatga
     2041 agatcgggac tccggcccag cgccagttgt cgatttccag acgcagcgcg gcgaaggtct
     2101 cgcgggtgga attggacttc acgccctcga tcttgcggta gccgttgtac tgcccgcgca
     2161 ccagattctg cgcggaaagc gggcgaatgc tcgccatgag cttggccttc tcgtcgcgca
     2221 tggcatgcgc gtcgctcgcc gccggcggct ccatcgcgag aatggaaagc tcctgcagca
     2281 tgtggttctg caccacgtcg cggatcgccc cgacttcgtc gtagaacgca ccgcggtctt
     2341 ccacgccgaa ggactcggcc atcgtgatct ggatgctctc gatgtaggtg cggttccaga
     2401 acggttcgag aaaggtgttg gcaaatcgga aatagagaag gttctgcacc ggctccttgc
     2461 cgagataatg atcgatgcgg tagatgcgct cttcggggaa tacctcgctg agagtgcgat
     2521 cgagcgcaag cgcggagtcg agatcgcggc cgaagggctt ttccacgacc acgcttcctt
     2581 tcttcgaaag cttcgccttc tgcaagcctt cgacgacggt cgcgaagaga ctcggcggga
     2641 tggcgagata aaagagcgga tgcgcggccc cgtcgagccg cttcgtgagt tcgccgagcg
     2701 tcttgtcgtc gcgatagtcg ccggcgatgt agtcgagacg ctggagaagt cgctcgaccg
     2761 cttttttgtc cttgttttcg ccgttttccg cgatgctttt gcgggcgcgc tcacgcagtt
     2821 gatcggccgt ccagtcggaa ctcgccactc cgatcaccgg catttccagt tcgcccgcga
     2881 tttcgaggcg ttcgagcgag ggaaaaatct tcttgtaggc gagatcgccg gtggcaccga
     2941 agagaacgag tgcgtccgat ccgtctgcgg tttccgcgct catggactca gtccttcttt
     3001 tcgtcgtggc cgccgaattc gctgcgcatc gccgagagga gcttgtcggc gaagccggcc
     3061 tcgccgcgac tggtgaagcg ctcgaaaagg gcgagggcga gcaccggcac cggggtgccg
     3121 gtttcgatcg cggcctgaat cgtccagcgt ccttcgccgg aatcggaaac gtgcccggcg
     3181 aagcgttcga gttcgggatc gcgggcgagc gcatcggcgg tgagatcgag cagccacgat
     3241 ccgattaccg agccgcgtcg ccagagttcg gcaatctcgg ggatgtcgag ttggtagcga
     3301 tagttgtcgg ggtcatcgag cgggctggtt tcggcatcct cgtcgtactt gcgggtgccg
     3361 gcgtcggcgt gacgcagcac gttgaagccc tcggcgaagg ccgccatgat gccgtactcg
     3421 atgccgttgt gcaccatctt gacgaagtgg ccggccccgg cctcgccgca acgaaggtag
     3481 ccctgcggcg cgctgccgct gcgcttgccg cgagagggcg tcggttcggc cgcctccacg
     3541 cccggagcga gggcgcggaa gatcggatcg agatgggcgc aggcgtcggc gttgccgccg
     3601 atcatgaggc agtagccgcg ttcgtaaccc cacactcctc ccgaaacccc cgcatcgagc
     3661 aaatgaatgc cgtcgccggc gagagtcttc gcctgacgga tcgcgtcctt gtaatgcgag
     3721 ttgccgccgt cgacgagaat gtcgccctcg gcgagcagcg gcctgagcgc ctcgatggtt
     3781 ttttcgacga aggccgtcgg gaccatcatc cacacgacgc gcggcgattt cagtttgccg
     3841 acaaaatcct cgagagaatc cgacgccgtc gcgccctcgg cggcgaggcg cttcaccgca
     3901 tccgcgctta cgtcgtagac gaccgcctga tgcccggcgt tgtgcagtcg cttgaccatg
     3961 ttggcgccca tgcgcccgag accgatcatt ccgaattgca tgagagactc cttgagaagc
     4021 tgcttccgct cccgctcccg gcagaagcgc agatgaaaaa atcaggcgtt cgccgtatcc
     4081 agaaagtcgt gaagcgcaac gcgaaacacg cgtcggtcgt gctccttgag tgcgaggaag
     4141 tcgcccgccg cctgcgcctc gaaaagctgc gcaaaaccat aggggcggcc gggaatttcg
     4201 agatccggcg cggtgtcgtc cacgatctgc acgaacacgc cgttggcatg tccgcccttg
     4261 tgcaactggc cggtcgaatg cagatagcga gggccgagtc cgagcgtcgt cgcgacgcgc
     4321 aaacggtcgc gcagtgcgag gcgtgccgtc tgcagttcgg cgaaatgctt ggagtcggtt
     4381 gcgacgtagg cctggatcgc gagatagtcg ccggacttga tcgccgcgag cgcatcgctc
     4441 gcggccgact gcggcagctc cggcactcct tcggcaagca ccttggaagc cgccttcttc
     4501 gcggcttcga cgttcggttg atcgaaggcg ttgatgccga gcacgtgacc ggcgatcgca
     4561 atcgcgaatt cccagcggaa gcactccgcg ccgatgctgt agcgatccac gtacggcagg
     4621 cggatcacgg gatgcgagtc ggcaacgaga ccgaggtcgg actgatctcc gagacagacg
     4681 aagaaacggt cgtcgccata gactcccggt ttgccgacgg tttcgccggc gacgggaagt
     4741 atgccgacgc cgtccttgcc ggtggactcc gcgatgagct gctcgagcca gcttccgaag
     4801 ctcgcgatct cgggcggtat caccagggtg agtttgtcgc agccctgctg cgaggcgaag
     4861 gcgatggccg cgcccagacg tacgccggga ttgcgctcgg cgggagtgtc cggcatcgaa
     4921 gcggcgatca tctgcccggc acggccgagg agttcttcca cgtcgacgcc cagcagcgcc
     4981 gcggcgacga gcccgaagtg ggagagggcg gagtagcgcc cgccgagatt gggattgttg
     5041 aagaaggtct tgcggaattt ccgctcggcc gcgatcttgc cgaggtccga gcccttgtcg
     5101 gtgatgacgg cgtagtgagc ggggtcgttc atccgcgacc agaaataatc gagatgacaa
     5161 cgcgtctcga gcgtaccgcc ggatttcgac gcgaacacga agagggtctt ttccagcggc
     5221 agcgaatcgg cggcctcgcg caccgtgacc ggattgctgg tgtcgagcac gatcatctcg
     5281 aggccgcccg catcgccgaa gctctgctgc atgacctgcg gaaagaggct cgatccgccc
     5341 attccgctcc agagcacatg cgtcatgccg gcgtcgcgca cgcccttgac gaattccttg
     5401 aggcgcggca cttcctccat catctcctgc ggcgaaccga gccagccgag ccggttgtcg
     5461 atctcgtccg gatccggctt ccacaccgta tggtcgtgcg cccagatgcg ctcgacgaca
     5521 ttgtctttcg cgatttcccc gatctgccgg cggatgccgg cctcaaattt tcccaggctt
     5581 tcgcgatcct cctcgctcgc ttcgcgaagc tcggcgagct tcttttcgat ctgcgcgagc
     5641 agttttgcat aggattcgtc gaacttcttt ttgccgtctt cctgcaggtc gcgagcgagc
     5701 ttgtccgtat ccacgccggc ctcgttgatt gccttgagcg tttccccggc gtcgccgccg
     5761 tcggcgggaa gtggctttcc gacccgcccg tccttcgcca cggaaaagag cgtggcttcc
     5821 gggatggtgt tgatggtgtc gggagcgacc agttcgtaca cgtaataatc gtcggagaga
     5881 ctcggatcct tggttccggt gctcgcccac agcaggcgtt gcgggctcgc gcctttcccg
     5941 gcgagcttct tccagcgatc ggatgcgtag agttcgcagt ggacgcgata ggtctctttg
     6001 ccgatcgcga cgccgagttt attctgaagt ttttcgggca gccggtccac gctcgccttg
     6061 tcccagcgcg agacgaagag cgaggcgacc gacggaacat gcgggtcctt gccttcggcg
     6121 atgcgccgct cgatcccttt cagatacgct tcggccgagc gccggtattg atcgggcgaa
     6181 aagagcaggg tgacgttgac gggaatgcct tcatagatga gttgctcgat cgcgccggca
     6241 ccggcttcgg tgcccggaac cttgatgaaa aggttcttgc gctgtgcgcg ccccgcgaga
     6301 tcgcgcgcct gacggatgga gccttcggta tcgtccgcaa ggcccggcgc gagttcgagc
     6361 gagacatacc catcgcgacc gtcgctcttt gcccagaacg ggagaaacag gtcggcggcc
     6421 tgctggagat cctcgatcgc gagttcgaaa aagatggcag cggcgtcggg atgttcatcg
     6481 tcgagatgcc ggcgaatacc ttcgtcgtat tcctcgccgc cgccgatcgc atgctcgaag
     6541 atcgtcgggt tggaagtgag accggtcagg gcgaactcct cgatgtaatg gcggagctga
     6601 ccgctcttga gcatgccgcg gcggatgttg tccatccaga tgctttcgcc gagtttgtgc
     6661 aattccttca gtgcattcat ttcatctccg tgtcagcgtt tcagcgacgg gccgttgcgg
     6721 tgaatcggag gcccgggatc gggccgcggc ggctccgttt ctctctttca gtgggcgcgc
     6781 ggagggcgcc cgagccgctt ctcgatctcc agtatacgct cggtagtcgc gagcaccgtg
     6841 tcgggattga ggctcatgga ttcgatgccg agttcgacca gaaattccgc catttccgga
     6901 taatccgacg gagcctgacc gcagagtccc gagtggattc cgttgcgctg gcagccctcg
     6961 accgcgaggc ggatcatgcg gcgcacgccc ggatcgcgtt cgtcgaaggc ggcggccacg
     7021 atttccgaat cgcggtcgac tccgagcgtg agctgcgtga gatcgttcga tccgatggaa
     7081 aagccgtcga agcgtgttgc gaactcgtcg atctgcatca cgttgttcgg aatttcgcac
     7141 atcgcgtaga tcttgagttc cttcgcgccg cgcttgagtc cgaacttctc catgagggcg
     7201 atgacgccgt cggcttccgc gacacgacgc acgaagggaa tcatgagaat cacgttgccg
     7261 aagcccatgg tttcgcgtac ccgcttcatc gcggcgcatt cgagttcgaa ggcctcggcg
     7321 taagccggat gggtgtagcg cgaagcgccg cgaaagccga gcatcggatt ctcttccttc
     7381 ggctcgaaat cagcgccgcc aacgagggcc gcatattcgt tgctcttgaa gtccgagagg
     7441 cgcacgacca ccgggcgggg ataaaaggcc gcggcgatcg tcgcgacgga ttccgaaagg
     7501 cgctcgatga aaaactcgcg gccattcgcg taacctgcga tgagacgccc tatctccctg
     7561 actgcggccg ggtccttcac cctttcggga tgcagcagcg cgagcggatg ggccttgatg
     7621 gattcgttga tgatgaattc gagacgggcg agcccgacgc cgtcgttggg cagaaaggaa
     7681 gtcttgaaag cgagcgccgg attgcccagg ttcaccatga tgcgcgtcgc cggacggggc
     7741 agggcggaaa gatcggtgcg cttgatctcg aagggaatct cgccgtcgta caccttgccg
     7801 gtatcgccct cggcgcagga caccgtcacg atcgcgccgt cttctatgag ccgcgtcgcc
     7861 tcggaggtcc cgacgatcgc gggaatgccg agttcccgcg caacgatcgc cgcgtgacag
     7921 gtgcgcccgc cgcgattggt gacgatcgcc gcggcgtttt tcatcaccgg ctcccagtcc
     7981 ggcgtcgtgg tctcgctcac gagcacctcg ccgggacgaa attcgtgcag cgccgaaaga
     8041 tcgcgcgcca cccgcacctt cccggaggcg atcttcgagc cgaccgcccg tccggtcacg
     8101 atcacctcgc cgctgccgcg gatgacatag tcctcgagaa tgccgccctt tcgctgcgag
     8161 gcgacggttt ccggcctcgc ctgcagcatg tagatcagcc cgtccttgcc gtccttcgcc
     8221 cattccatgt ccatcggacg gggcacaccg gcgcgctcgc tgtagtggcg ttcgatcttg
     8281 atcgcgtagt cggcgaggag cagcacgtcc tcgtcgctca gggaaaagcg ccggcgtttt
     8341 tcttccggcg tctcgacgtt cttcacccgc gcgggatcgc ccgcatcggc gtagatcatg
     8401 gtgaggtgtt tgctcccgag gcgccggcgc aggacc
//
LOCUS       DASUJG010000150         6740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_532563, whole genome shotgun sequence.
ACCESSION   DASUJG010000150 DASUJG010000000
VERSION     DASUJG010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6740
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532563"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1179)
                     /gene="tdh"
                     /locus_tag="VFX38_03140"
     CDS             complement(154..1179)
                     /gene="tdh"
                     /locus_tag="VFX38_03140"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006534211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEX5313888.1"
                     /translation="MRALVKAKKAEGIWAEDVPRPEIGHNDVLIRIKKTAICGTDIHI
                     YDWNEWAQKTIPVPMTIGHEFVGEIAELGSEVKGLEIGQRVSGEGHITCGHCRNCRAG
                     RRHLCPNTKGIGVNRPGAFAEYLSLPAVNVYPLPDSVPDDIAAFLDPFGNATHTALSF
                     DLVGEDVLITGAGPIGCMAAAIARHCGARRVVVTDVNDYRLALAAKMGADRAVNVASE
                     KLEDAMKKLGLREGFDVGLEMSGNPQALHQMLAVMVNGGKVALLGLPPGKIELDLNQV
                     IFKGLFLKGVYGREMFETWYKMIAMLESGLDISPVITHRLPIARFQEGFDVMRSGKSG
                     KVVLDWT"
     gene            complement(1384..2571)
                     /locus_tag="VFX38_03145"
     CDS             complement(1384..2571)
                     /locus_tag="VFX38_03145"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018623542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEX5313889.1"
                     /translation="MPRPDLSFLDRAVEELKAEGLYKAERILAGRQGAEIEVGGRRVL
                     NLCANNYLGLAGHPELVKAAQQALERYGYGMASVRFICGTQDVHKALEAKLAGFLGVE
                     DAILYSSCFDANGGLFETLLDEQDVIVSDALNHASIIDGVRLCKANRLRYANNDMAEL
                     EARLKEAKDARLRLIATDGVFSMDGIVANLRGICDLADKYEALVMVDDSHATGFMGKN
                     GRGSHEYRDVMGRIDLITTTFGKALGGASGGATAGRRELIAWLRQRSRPYLFSNSLAP
                     AITATTLHVLEMLESGSELRERLHDNARYFRKGMSALGFDLVPGEHPIIPVMLGDAKL
                     ATEMADRLLGEGVYVIGFSFPVVPKGKARIRCQMSAALTRDELDRAIAAFGKVGRELK
                     VIP"
     gene            2694..3683
                     /gene="lipA"
                     /locus_tag="VFX38_03150"
     CDS             2694..3683
                     /gene="lipA"
                     /locus_tag="VFX38_03150"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX5313890.1"
                     /translation="MKRLADIPVVKSGRKFTTPQGFTAIKNGIRARASAPPPDIFLRK
                     PAWLRVKLPSGEGYEAVKANVRAHRLATVCEESMCPNIAECWNAGTATIMIMGSVCTR
                     ACRFCAVDTGNPRGWLDPDEPAHVADSVRLMGLKYVVMTSVDRDDLADGGAGHYAACV
                     HAIRSMNPQTAVEALTPDFRGNHDAVAKVVESDLQVYAQNLETVDRLTHEVRDPRAGY
                     RQTLDVLAFAKQYRPAVLTKTSLMLGLGERDEEIAQALDDCRAAGVDIVTFGQYLRPT
                     KNHLPVVRYVPPEDFARYREWGLERGFLEVVAGPLVRSSYRAERVFERNNLGL"
     gene            3773..4762
                     /locus_tag="VFX38_03155"
     CDS             3773..4762
                     /locus_tag="VFX38_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX5313891.1"
                     /translation="MQAMVLEEGAHRLVERDLPRPEPKAGEILVRVLACGVCRTDLHI
                     VDGELVPPRWPVIPGHEVVGEVAGLGEGTTGFEIGDRVGLAWLAGSDGECEYCRRGEE
                     NLCPNAAFTGFARPGGYAEYTTARADFAYQLPADADPVATAPLLCAGLIGYRTWRKAG
                     GGERIGLYGFGAAAHILAQLLVREGKIVYAFTRPGDTTSQAFARRLGAAWAGGSDEKP
                     PESLDAALIFAPVGALVPAALEAVRPGGRVVSGGIHMSDIPAFPYRLLWEERSVASVA
                     NLTRADGREFLALAAKHPIHTTTKRYPLGSANEALDDLRAGRLEGAAVLVPDL"
     gene            4871..5359
                     /locus_tag="VFX38_03160"
     CDS             4871..5359
                     /locus_tag="VFX38_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5313892.1"
                     /translation="MHESFAVPEGSKPVVYAALARHAEALVEGEGDKIANAANLAALL
                     YHGLPDVSWCGFYFLRGKQLIVGPFQGKPACVRIALGRGVCGQAATMRRTLVVGDVAS
                     FPDHIYCDPDARSEIVLPLVKDDKLLGVLDLDAPILDRFDEQDRRGLEKIATIWAQSF
                     RR"
     gene            5436..6056
                     /gene="lexA"
                     /locus_tag="VFX38_03165"
     CDS             5436..6056
                     /gene="lexA"
                     /locus_tag="VFX38_03165"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012442797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX5313893.1"
                     /translation="MDALTSRQAEILELIRETLRARGIPPTRAEIAARLGFRSPKAAE
                     DHLRALARKGAIELLPGTSRGIRLRFAAAAKDELRLPLVGRVAAGAPILAVEHVEAEY
                     RVDPRLFRPRADYLLRVHGESMSGAGIRDGDCLAVHRSAEAEDGRIVVARLDDEVTVK
                     RLRRRGRRIELLPANPDFAPIRVNPERQSFAIEGLGVGIIRNGNPL"
     gene            6053..6649
                     /gene="imuA"
                     /locus_tag="VFX38_03170"
     CDS             6053..6649
                     /gene="imuA"
                     /locus_tag="VFX38_03170"
                     /inference="COORDINATES: protein motif:HMM:NF033429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
                     /protein_id="HEX5313894.1"
                     /translation="MIVSALEAVLARPDVWRGAAAHWAETLPSGLAALDAALGGGWPL
                     GVLGDCLVQCEAGGMALFLPALARLTREHWAVLISPPHVPYAPALAQAGVDLSRLLVL
                     RPPSDERLWAALQCLESGACSAVFLWGGDGGLRDFRRLQLAAEESRVLAVLFHVRDEH
                     RPAALRLAVRPRPEGGLVVELLKCRGRPARRIELPDAV"
BASE COUNT         1090 a         2134 c         2275 g         1241 t
ORIGIN      
        1 ccgcgacgct gagagagaca tccccctcga tcccccttcc ttcggaaggg ggaagctgcc
       61 gtgggtcttc cttcggagga gcgaggagtc agcaattgca gttcggaagg gggaggagtg
      121 agcaagcttc ttcggaaggg gaaagcgaca atgtcaggtc cagtcgagga cgaccttgcc
      181 ggatttgccg ctgcgcatca cgtcgaagcc ttcctggaag cgagcgatcg ggagccgatg
      241 ggtgattacc ggtgagatgt cgaggccgct ctcgagcatc gcgatcatct tgtaccaggt
      301 ctcgaacatt tcgcggccgt acacgccctt caggaacagg cccttgaaga tgacctggtt
      361 cagatcgagt tcgattttgc ccggcggcag gccgagcagc gcgaccttgc cgccgttgac
      421 catgacggcg agcatctggt gcagcgcctg cggattgccg gacatttcga ggccgacgtc
      481 gaagccctca cgcagcccga gttttttcat tgcgtcttcg agcttttccg atgccacgtt
      541 gaccgcgcga tcggcgccca tttttgcggc gagtgcgagc cggtagtcgt tgacgtcggt
      601 gacgacgacg cgccgtgcac cgcaatgacg cgcgatggcc gccgccatgc aaccgatcgg
      661 accggcgccg gtgatgagca cgtcctcgcc gacgagatcg aaggacaggg ccgtgtgagt
      721 cgcattgccg aaggggtcca gaaaggcggc gatgtcgtcg ggcacggagt cgggcagggg
      781 atagacgttc accgcaggaa gcgaaaggta ttcggcgaag gcgccgggtc ggttgacacc
      841 gatgcccttc gtgttcggac aaagatggcg gcgtccggcg cggcagttgc ggcagtgccc
      901 gcaggtgatg tgcccttcgc ccgacacgcg ctggccgatc tcgaggccct tgacttcgct
      961 tccgagttcg gcgatctcgc cgacgaactc gtgaccgatc gtcatcggca cgggaatcgt
     1021 tttttgtgcc cattcgttcc agtcgtagat gtggatgtcg gtgccgcaga tcgcggtctt
     1081 cttgatgcgg atcagcacat cgttgtggcc gatttctgga cgcggaacgt cctcggccca
     1141 gatgccttcg gccttttttg cttttaccaa tgcgcgcatt tatgcaatga ctctgctcga
     1201 taccctctcc cccgagggga gagggatggg tgaggggctt ccccgctttg cggcgaagag
     1261 tgtgtcttca ccctcacccc aaccctctcg cttgagggga gagggggaag aagtgcggcc
     1321 tccctgcaag ggagaggggg aagatagatc acggcccgcc ggtgaggtaa cgaagaaaaa
     1381 cattcaggga atgaccttga gttcgcggcc gaccttgccg aaggcggcga tcgcgcggtc
     1441 gagctcatcg cgggtgagcg cggccgacat ctggcagcgg atgcgcgcct tgcccttggg
     1501 tacgacgggg aaggagaagc cgatcacgta cacgccttcg ccgagcaggc ggtcggccat
     1561 ctccgtcgcg agcttcgcat cgccgagcat gaccggaatg atcgggtgtt cgccggggac
     1621 gagatcgaag ccgagcgcac tcatgccctt tcggaagtag cgcgcattgt cgtgcaggcg
     1681 ctcgcgcagc tccgaacccg attcgagcat ctcgagcacg tgcagcgtcg tcgccgtgat
     1741 ggccggtgcg agcgaattgg aaaaaagata cgggcgcgaa cgctggcgca gccaggcgat
     1801 gagttcgcgc cgccccgcgg tcgcgccgcc cgaggcgccg ccgagcgcct tgccgaaggt
     1861 ggtggtgatg agatcgatgc gacccatcac gtcgcggtat tcgtggcttc cgcgtccgtt
     1921 cttgcccatg aaaccggtcg cgtgcgaatc gtccaccatg acgagcgctt cgtatttgtc
     1981 ggcgagatcg cagatgccgc gcaggttggc gacgatgccg tccatcgaaa acacgccgtc
     2041 ggttgcgatc aggcgcaggc gcgcgtcttt tgcttccttg agacgcgctt ccagttccgc
     2101 catgtcgttg ttggcgtaac gcagccgatt cgccttgcac aggcgcacgc cgtcgatgat
     2161 gctcgcgtgg ttgagcgcat cggaaacgat gacgtcttgc tcgtcgagca gggtttcgaa
     2221 caggccgccg ttggcgtcga agcaggagga gtagagaatc gcatcttcca cgccgagaaa
     2281 gcccgcgagt ttcgcttcga gcgccttgtg cacgtcctgg gtgccgcaga tgaaacgcac
     2341 cgaggccatg ccgtaaccgt agcgttcgag cgcctgctgc gccgccttga cgagttcggg
     2401 gtggccggcg aggccgagat agttgttggc gcagaggttc agcacgcgcc ggccgccaac
     2461 ctcgatttcc gcaccctggc ggccggccag aatgcgttct gccttgtaga gaccctcggc
     2521 cttcagttct tcgacggcgc gatcgagaaa ggagagatcc ggtcgcggca tgctgcactc
     2581 ctgcggaaac cccgctcatt atactttcgc tcgccgcgcg agccgggcgc ggccccaagg
     2641 tcggcgccga ggtgggataa tacgcgcatg cacgcattct tccggcccgg cccatgaagc
     2701 gcctcgccga cattccggtg gtgaaaagcg ggcgcaagtt cacgacgcca cagggcttca
     2761 ccgcgatcaa gaacggcatt cgcgcgcgcg cctcggcgcc gccaccggac attttcctgc
     2821 gcaagcccgc gtggttgcgg gtgaagcttc cgagcggcga aggctacgag gccgtgaagg
     2881 ccaacgtgcg cgcgcatcgc ctcgcgaccg tttgcgagga gtccatgtgt ccgaacatcg
     2941 ccgaatgctg gaacgccggc acggcgacca tcatgatcat ggggtcggta tgtacgcggg
     3001 cctgccgctt ctgtgcggta gataccggca atccgcgcgg ctggctcgat cccgacgagc
     3061 ccgcgcatgt ggccgattcg gtgcggttga tgggattgaa gtatgtcgtg atgacgtccg
     3121 tggatcgcga cgatcttgcc gatggcggtg cgggacatta cgccgcctgc gtacatgcta
     3181 tccgctccat gaatccgcag acggcggtcg aggcgctgac gccggatttc cgcggcaatc
     3241 acgacgccgt cgcgaaggtc gtggaatcgg atctccaggt ctatgcgcag aatctcgaaa
     3301 ccgtggatcg cctgacgcac gaggtgcgtg acccgcgcgc gggctatcgc cagacgctcg
     3361 acgtgctcgc cttcgccaaa caatatcgcc ccgcggtgct gaccaagacc agcctcatgc
     3421 tcgggctcgg cgaacgcgac gaggaaatcg cgcaggccct cgacgattgc cgcgcggcgg
     3481 gcgtggacat cgtcaccttc ggccagtatc tgcgaccgac gaagaatcat ctgccggtgg
     3541 tgcgctacgt tccgccggaa gatttcgcgc gctatcgcga atggggactc gagcgcggtt
     3601 ttctcgaagt cgtcgccggg ccgctggtgc gttcgagcta tcgtgcggag cgggtgttcg
     3661 agcgcaacaa cctcggcctg tgatttcaat ttgtgggagc gagcttgctc gcgataacat
     3721 cgcgtattag gattcgcgag caagctcgct cccacgcagc cttatgaaca aaatgcaggc
     3781 gatggtgctg gaagagggtg cgcaccggct ggtcgaacgc gatttgccgc gtcccgaacc
     3841 gaaggcggga gaaatcctgg tgcgcgtgct cgcctgcggt gtctgccgca cggatctgca
     3901 catcgtggac ggcgagctgg ttccgccgcg ctggcccgtc atccccggtc acgaagtcgt
     3961 cggtgaagtc gcgggtctcg gcgagggcac aaccggcttc gagatcggcg atcgcgtggg
     4021 gctcgcctgg ctcgccggca gcgacggcga gtgtgagtat tgccgccgcg gcgaggagaa
     4081 tctgtgcccg aacgccgcgt tcaccggctt tgcgcgcccc ggcggctacg cggagtacac
     4141 gacggcgcgc gccgatttcg cctaccaatt gccagccgac gccgacccgg tcgcgacggc
     4201 gccgctcctg tgtgcggggt tgatcggcta tcggacctgg cgcaaggccg gcgggggcga
     4261 acgcatcggg ctgtacggct tcggcgccgc cgcgcacatc ctcgcgcagt tgctcgtgcg
     4321 cgaaggcaag atcgtgtacg ccttcacgcg tcccggcgat acgacttcgc aggcgtttgc
     4381 gcgccggctc ggcgccgcat gggccggggg ttccgacgaa aaaccgccgg agtcgctgga
     4441 tgcggcgttg atcttcgctc cggtcggtgc gctggtaccc gctgcactcg aagccgtacg
     4501 tcccggcggc cgtgtggtga gcggcggaat tcacatgtcc gatattccgg cgtttccgta
     4561 tcgactgttg tgggaggagc gcagcgtcgc ttcggtcgcg aatctcaccc gcgccgacgg
     4621 acgcgaattt ctcgcgctcg ccgcgaagca tcccattcac accacgacga agcgctatcc
     4681 attgggctcc gcaaacgaag cgctcgacga cttgcgcgcc ggccgcctcg aaggcgccgc
     4741 cgtcctcgtg ccggatctgt agaagcaggc ttgctcgcga aatcaaacgt gtgggaaagg
     4801 aatcgcgaac aagctcgctc ccacccaatc ggcggacgcg atttgcgggg gttgcggtag
     4861 gctgggcggc atgcacgaat cgtttgccgt gcccgagggc tcgaaaccgg tggtgtacgc
     4921 ggcgctggcg cgtcatgccg aggcgctcgt cgaaggggag ggcgacaaga tcgcgaatgc
     4981 cgcgaatctc gccgcgcttc tctatcacgg gctccccgac gtaagttggt gcggcttcta
     5041 ctttttacgc ggcaagcagt tgatcgtagg gccattccag ggaaaaccgg cctgcgtgcg
     5101 gatcgcgctg ggacgcgggg tctgcggtca ggccgcgacc atgcgtcgca cgctggtggt
     5161 gggggatgtg gcgagttttc ccgaccacat ctactgcgat ccggacgcac gttcggagat
     5221 cgtgctgccg ctggtgaagg acgacaaact cctcggggtg ctcgatcttg atgcgccgat
     5281 tctcgatcgc ttcgacgagc aggaccggcg cggcctggag aaaatcgcga ccatctgggc
     5341 gcaatccttc cgccgctgag ccgttcgtcc gcgcgcccat ttgcacgagg cgcccgaata
     5401 ctatataaat aaccagtacc cccgttcggg cactcatgga cgcactgact tcccggcagg
     5461 ccgaaatcct ggagctgatc cgcgaaactc tgcgggcccg tggcatcccg cccacccggg
     5521 cggagatcgc cgcgcgtctc ggcttccgct cccccaaggc cgccgaagat catctgcgcg
     5581 cgctcgcccg caagggcgcg atcgaactgc tgccgggcac ctcgcgcggc atccgcctgc
     5641 gtttcgccgc ggccgcaaag gacgaattgc gcctgccgct cgtcgggcgg gtggcggccg
     5701 gagcgcccat cctcgccgtc gaacatgtcg aggccgagta tcgcgtggac ccgcgcctct
     5761 tccgtccccg cgccgattac ctgctgcggg tgcacggcga gagcatgagc ggagccggga
     5821 tccgcgacgg cgactgtctt gccgtgcatc gcagcgcgga ggcggaggat ggccggatcg
     5881 tggtcgcgcg gctggacgac gaggtgacgg tcaagcgtct tcgccgccgc ggccggcgga
     5941 tcgaactgct gcccgccaat cccgacttcg cgccgatccg cgtgaatccc gaacgccaga
     6001 gcttcgcgat cgagggcttg ggcgttggca tcatccgcaa cggaaatccg ctgtgatcgt
     6061 atctgcactc gaagcggtgc tcgcccgtcc ggatgtctgg cggggcgccg ccgcgcactg
     6121 ggccgaaacg cttcccagcg gtttggccgc gctcgatgcc gcgcttggcg gcggctggcc
     6181 gctcggagtt ctgggcgatt gtctcgtgca atgcgaagcc ggcggaatgg cgctttttct
     6241 gccggcgctc gcgcggctca cacgcgaaca ttgggccgtg ctgatttcgc cgccgcatgt
     6301 tccctatgcg ccggcactgg cgcaggcggg agtggatctg tcgcgtcttc tggttcttcg
     6361 cccgccgtcg gacgagcgcc tgtgggcggc gctgcaatgt ctggaaagcg gcgcctgttc
     6421 cgccgtgttt ctctggggtg gcgatggcgg tctgcgcgac tttcgccgcc tgcaactcgc
     6481 ggcggaggaa agtcgtgtcc tcgccgtgct gttccatgtc cgcgatgagc accggcccgc
     6541 cgccctgcgt ctcgccgttc gcccgcgtcc cgaaggcgga ctcgtcgtgg aattgctgaa
     6601 gtgccgcggg cgtccggcgc ggcgtatcga attgccggac gccgtctgag cgatccgtct
     6661 tgcgcattca tgccgcgttc gcttccgctt ccgctttcct tgccggcacc gtcgccggcc
     6721 gcggcgtttg ccccgccgcc
//
LOCUS       DASUJG010000151         5384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1144418, whole genome shotgun sequence.
ACCESSION   DASUJG010000151 DASUJG010000000
VERSION     DASUJG010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5384
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144418"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VFX38_03175"
     CDS             complement(<1..244)
                     /locus_tag="VFX38_03175"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018623683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX5313895.1"
                     /translation="MKPVLTVTVEVEVPFQDLDPLAIAWHGNYFRYFEAARSGLFRRL
                     DYDYPQMEASGYAWPLVEVKARFLRPARYAQRLAIEA"
     gene            complement(241..1785)
                     /locus_tag="VFX38_03180"
     CDS             complement(241..1785)
                     /locus_tag="VFX38_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HEX5313896.1"
                     /translation="MSERRAVLVGGDALTLEDLGAIATGAPVALDAGPAFRGRIEAGV
                     RAVAAALAGGQPIYGVTTGYGDSCDRAVPPELLAELPVQISRYHGCGLGRHFDAPTTR
                     AILLARLVSLAHGYSGVRLEVLESLAALLNHDVLPLIPEEGSVGASGDLTPLSYVVAA
                     LIGEREVVNGGQLVGAAEALATAGLAPLTLAPKEGLALMNGTAVMTALAALGWLRAEY
                     LARLAARITALAALALRSNAEHFRAELFAAKPHPGQAAIAARIHEDLAANGEQDRPAR
                     IQARYSIRCAPHVIGVLADALPWMRAWLETELNSANDNPLVDPASGEIYHGGHFYGGH
                     VAFVADALKAAVANVADLLDRQLALLVDLHYNHGLPRNLSGATGERAVLQHGLKAVQI
                     AVSAWTAEALKLTMPASVFSRSTECHNQDKVSMGTIAARDALRVLELTEQVAAACLFA
                     TCQATALAERDNGFALRAPALSATREEVFRIAPFLDEDRPLERELRALVDALRARHWP
                     LGELLA"
     gene            complement(1778..3454)
                     /locus_tag="VFX38_03185"
     CDS             complement(1778..3454)
                     /locus_tag="VFX38_03185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5313897.1"
                     /translation="MSLRVCVLIPAYNHHRELERLLLRLLAQGLPVLLVDDGSGAETR
                     EALKALAMRYPAVRLHRLACNGGKGAAVLAGLAVLEAAGYSHALQIDADGQHDPDEAA
                     VLLEAARHEPAALVSGVPRFDASVPRLRFYGRYLTHALVWLETLSFTIPDSMCGFRVY
                     PLAATLAVARATHIGRRMDFDTEIMVKLRWAGTPLRFLPVAVRYPADGISHFRMFADN
                     ARLTWMHIRLIAGMLIRSTRLVFRGTAPRWDEVREAGSYAGLRLLAIVWRWGGRRFAQ
                     ALLAPAVVWFFLVHGEVRRGSRRYLATIHECTEEKWRRRTRPTRRNVLRHLWHFADAG
                     LDKYAIWAGLPAPPVRFPHQEIFLAEVRKGQGVLLLAAHLGNFEVARAIVGDWPEVRL
                     NSIFYTGNARKFARFMQGSSVAYGERLIETAGPDVATGMLLSERIGAGEVIALVGDRT
                     PRGARDGVIHAPFLGRSAPFPTGPYLLAHALGCPVYLSLCLREEDGYSVYCEPFAERI
                     RLGREGRREELASLVARYAGRLEHYARSHPLDWFNFFDFWRPVPGVPAHE"
     gene            complement(3451..3804)
                     /locus_tag="VFX38_03190"
     CDS             complement(3451..3804)
                     /locus_tag="VFX38_03190"
                     /inference="COORDINATES: protein motif:HMM:NF019589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313898.1"
                     /translation="MRLPRVVARETGPGSLRLTLAVDPDLDWFAGHFPGQPVLPGIAQ
                     IGWAIAFAREGFGLAADPPGLDQVKFLRPVEPGATLVLELKYNEGLRRVRYRLTNGED
                     TVGSGRLDFGSGETP"
     gene            complement(3801..5201)
                     /locus_tag="VFX38_03195"
     CDS             complement(3801..5201)
                     /locus_tag="VFX38_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX5313899.1"
                     /translation="MTTELLDAGSRLLARPQSLGSRLAGLAQSERPVAFATAGSISGA
                     KFSAAARGLAALLAMRPERAWALVAEDSVDFARGFFALAAAGKTILLPQGARPEAIAI
                     TGAEAILSDGRESAPELPRVALDGATMTDTTLADDFDPAAVAIGIHTSGSTAQPKRVT
                     KTLAQLDAEIVALEAEWGSLLGDAPVIASVPHHHLYGLLVSLLWPLAAGRPILDFPAE
                     FSARTADCVLVSSPAFLGRIARPEALRAFPPRAIFSSGAPLPAAAAIAIAETAGPAPI
                     EIYGSTETGGIGWRARASAAETDAPWRRISGIELRFREEENGSRLLVRSPWTAGEWIA
                     TGDFAVALDDSRFRLAGRADGMIKLEDKRINPEELAAFLEEHELVHRARVIALDGASR
                     RRLGAVVVPTPAGSRMLETEGKANFVARLDARLAERYERVLIPRKWRIVAALPVNAMG
                     KIERARLAALFDGESA"
     gene            complement(5198..>5384)
                     /locus_tag="VFX38_03200"
     CDS             complement(5198..>5384)
                     /locus_tag="VFX38_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005297935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313900.1"
                     /translation="AFNTLLAFLTARWGSLAEWTLYNGFLSYLLIGGFWGGEMLFRRR
                     IRRRWAAAESSGAMLAR"
BASE COUNT          921 a         1766 c         1928 g          769 t
ORIGIN      
        1 ccgcctcgat cgcgagccgc tgcgcgtaac gcgccgggcg cagaaagcgc gccttgacct
       61 cgaccagcgg ccaggcgtac ccggaggcct ccatttgcgg gtaatcgtag tcgaggcggc
      121 ggaagagtcc ggagcgcgcc gcctcgaaat agcggaagta gttgccgtgc caggcgatcg
      181 cgagcggatc gagatcctgg aaggggactt ccacctcgac cgtgaccgta agcaccggct
      241 tcacgcgagc agctcgccga gcggccagtg acgcgcgcgc agcgcatcca ccaatgcgcg
      301 aagctcacgt tccagcgggc gatcctcgtc aagaaaagga gcgatgcgaa acacttcctc
      361 gcgcgtcgcc gagagtgcgg gcgcgcgaag tgcaaagccg ttgtcgcgct cggcgagcgc
      421 cgtcgcctga caggtggcga agaggcaggc cgccgcgacc tgctcggtga gttcgagcac
      481 gcgcagcgca tcgcgggcgg cgatcgtgcc catgctcacc ttgtcctggt tgtggcattc
      541 cgtggagcgc gaaaagacgc tcgccggcat cgtgagtttc agcgcctcgg cggtccaggc
      601 ggacacggcg atctgcacgg ccttgagccc gtgctgcagc acggcgcgct cgccggtcgc
      661 gccggaaaga ttccgcggca ggccgtggtt gtaatgcagg tccacgagga gcgcgagctg
      721 gcgatcgagc agatcggcga cgttggcgac cgcggccttg agtgcatcgg cgacgaaggc
      781 aacgtggccg ccgtagaaat gcccgccgtg ataaatctcg ccgctcgcgg gatccacgag
      841 cggattgtcg ttggcgctgt tgagttcggt ttcgagccag gcgcgcatcc acgggagcgc
      901 atcggcgagc acaccgatca cgtgcggtgc gcagcggatg gaatagcggg cctggatgcg
      961 cgccgggcga tcctgctcgc cgttggccgc gagatcctcg tgaatgcgcg cggcgatcgc
     1021 ggcctgaccg ggatgcggtt tcgccgcgaa caactcggcg cggaaatgtt cggcgttgct
     1081 gcgcagcgcg agcgccgcga gcgccgtgat tcgagcggcg aggcgcgcga gatattccgc
     1141 acgcaaccag ccgagcgcgg cgagtgcagt catgaccgcc gtgccgttca tgagggcgag
     1201 gccttccttg ggcgcgagcg tgagcggtgc gagccccgct gtcgcgagcg cttcggcggc
     1261 gccgacgagt tgccccccgt tcacgacttc gcgctcgccg atgagcgccg cgacgacata
     1321 ggaaagcggc gtcagatcgc cgctcgctcc gaccgatcct tcctcgggaa tcagcggcaa
     1381 cacgtcgtga ttgaggagtg cggcgagact ttcgagcact tcgaggcgca cgcccgagta
     1441 gccgtgcgca agcgatacga gccgggcgag aagaatcgca cgggtcgtcg gtgcatcgaa
     1501 atgacggccg aggccgcagc cgtgatagcg gctgatctgg accggaagct ccgcgagcag
     1561 ttcgggcggc acggcccggt cgcaggaatc gccatagccc gtggttacgc cgtagatcgg
     1621 ctgcccgccc gcgagggccg ccgcgacggc gcgcacaccg gcctcgatcc ggccgcggaa
     1681 tgcggggccg gcatcgagtg cgacgggcgc tccggtggcg atcgcgccga gatcctcgag
     1741 cgtgagcgcg tcgccgccga cgagtaccgc ccggcgttca ctcatgggcc ggtactccgg
     1801 gcaccggccg ccagaaatcg aagaagttga accagtccag gggatggctc ctcgcgtagt
     1861 gttccaagcg tccagcatag cgggcgacga ggctcgcgag ttcttcgcgc cggccctcgc
     1921 ggccgaggcg gatgcgctcg gcgaagggtt cgcaatagac actgtacccg tcctcttcgc
     1981 gaaggcagag cgacaggtac acgggacagc cgagtgcatg cgcgagcaga taggggccgg
     2041 tcgggaaggg ggcggagcgc ccgagaaagg gcgcatgaat cacgccgtcg cgggcgccgc
     2101 ggggcgtgcg atccccgacg agtgcgatca cctcgccggc gccgatgcgc tcggaaagga
     2161 gcatgccggt cgccacatcc ggtccggccg tttcgatgag gcgttcgccg tacgccacgc
     2221 tgcttccctg catgaagcgc gcgaacttgc gggcgttgcc ggtgtagaag atggaattca
     2281 ggcgaacctc gggccagtcg ccgacgatcg cgcgcgcgac ctcgaagttg ccgagatgcg
     2341 ccgcgagcag cagcacgccc tggcccttgc gcacctcggc gaggaaaatc tcctgatggg
     2401 ggaagcgcac cggcggcgcc ggaagccccg cccagatcgc gtacttgtcg agtccggcat
     2461 cggcaaaatg ccacagatgc cgcagcacgt tgcgcctcgt cgggcgcgtg cgccgtcgcc
     2521 atttctcctc ggtgcattcg tggatcgtcg cgagatagcg gcgcgagccg cggcgcacct
     2581 caccgtgcac gagaaaaaac cagaccacgg ccggggcgag cagagcctgg gcgaagcgcc
     2641 gcccgcccca gcgccagacg atggcgagga ggcgcaggcc cgcataggag ccggcctccc
     2701 gcacttcgtc ccagcgcgga gcggtgccgc gaaagacgag ccttgtcgat cgaatcagca
     2761 tgccggcgat gagtcggata tgcatccagg tgaggcgggc attatcggcg aacattcgga
     2821 aatgcgaaat gccgtcggcc ggatagcgca ccgcaaccgg caggaaccga agcggcgtgc
     2881 cggcccagcg aagcttcacc atgatttcgg tatcgaagtc catgcgccgc ccgatatgcg
     2941 tggcgcgcgc gacggcaagc gtcgccgcga gcggatagac gcgaaagccg cacatggagt
     3001 cgggaatggt gaaggagagc gtctccagcc acaccagcgc gtgggtgagg tagcgcccgt
     3061 aaaagcgcag gcgcggcacg gacgcgtcga agcgcggaac gccggaaacc agcgccgccg
     3121 gttcgtgcct ggcggcttcg agaaggacgg ccgcttcatc gggatcgtgc tggccgtcgg
     3181 cgtcgatctg cagcgcatgg ctgtagcccg ccgcttcgag tacggccagc cccgcgagca
     3241 ccgccgctcc cttgccgccg ttgcacgcga ggcgatgcag acggaccgcc ggatagcgca
     3301 tggcgagggc cttcagggct tcgcgcgttt ccgcgccgct gccatcgtcg acgagcagca
     3361 ccggcaggcc ttgcgccagc agacgcaaaa gcagccgctc gagttcgcgg tgatggttgt
     3421 aggccgggat gaggacgcag acgcgcaggc tcagggcgtt tctccgctgc cgaagtcgag
     3481 tcttccgctg ccgaccgtgt cctcaccgtt cgtcagacgg tagcggacgc gccgcaatcc
     3541 ttcgttgtat ttgagttcga gcacgagcgt tgcgccgggc tccaccgggc gcagaaactt
     3601 cacctggtcc aatccgggcg gatcggccgc gaggccgaag ccttcgcggg cgaaggcgat
     3661 ggcccaaccg atctgcgcga ttccgggaag cacgggctgg ccgggaaaat gccccgcgaa
     3721 ccagtcgaga tcgggatcga cggcaagggt gagacgcagg cttcccggac ccgtctcgcg
     3781 ggccacgacg cgcggcagtc tcatgcgctc tcgccgtcga agagcgcggc gagccgcgca
     3841 cgctcgatct tgcccatggc gttgaccggg agtgccgcga cgatccgcca cttgcgcggg
     3901 atcagcacac gttcgtagcg ttcggcgagc cgggcatcga gccgcgcgac gaaattcgcc
     3961 ttgccttccg tttccagcat ccgcgaacct gcgggcgtcg gcacgacgac ggcgccgagg
     4021 cgacggcggc tggcgccatc gagggcgatc acccgcgccc tgtgcaccag ctcgtgctcc
     4081 tcgagaaagg cggcgagttc ttccggattg atgcgcttgt cctcgagctt gatcatgccg
     4141 tcggcgcggc cggcgaggcg aaagcgcgag tcatcgagcg ctaccgcgaa atcccctgtc
     4201 gcgatccact cgcccgccgt ccagggggag cgaactagca ggcggctgcc gttttcctct
     4261 tcgcggaagc gcaactcgat gccggaaatt ctccgccacg gcgcatcggt ctcggcggcg
     4321 ctcgcgcggg cgcgccagcc gatgccgccg gtttcggtgc tgccgtagat ttcgatcggc
     4381 gcaggcccgg cggtttccgc aatcgcgatc gcggcggccg cgggaagcgg tgcgccggag
     4441 ctgaaaatcg cccgcggcgg aaaggcccga agcgcctcgg gccgcgcaat gcgaccgaga
     4501 aaggcggggc tcgacacgag aacgcaatcc gcggtccgcg cgctgaattc ggccgggaag
     4561 tcgaggatcg gccgtcctgc cgcaagcggc cagagcaggg acacgaggag cccgtagaga
     4621 tgatggtgcg ggacgctcgc gatcaccggc gcatcgccga gcagcgatcc ccattcggct
     4681 tcgagcgcga cgatctcggc gtcgagttgc gccagggttt tggttacgcg tttgggctgc
     4741 gccgtgcttc ccgaggtatg gatgccgatc gcgacggcgg cgggatcgaa gtcgtcggca
     4801 agagtcgtgt ccgtcatcgt cgcgccatcg agcgcgacgc gcggaagctc cggagcggat
     4861 tcgcgcccgt cgctcaggat cgcttccgcg ccggtgatcg cgatggcttc cggacgcgcg
     4921 ccctgtggca ggaggatcgt cttgccggcc gcggccagtg cgaagaatcc gcgggcgaag
     4981 tcgacactgt cttcggcaac cagcgcccag gctcgttccg ggcgcatggc cagcagtgcg
     5041 gcgaggccgc gtgccgcggc ggaaaatttt gcgccgctga tgctgccagc ggttgcgaag
     5101 gcgacggggc gctccgattg cgcgaggccc gcgagccgcg aaccgagaga ctgcggccgc
     5161 gcgagcagcc gcgagccggc gtcgagaagc tccgtcgtca tctcgcgagc atcgcgccgg
     5221 aggactccgc ggccgcccag cggcgacgga tgcgccggcg gaacagcatt tccccgcccc
     5281 agaagccacc gatcaggaga taagaaagga aaccgttgta gagagtccat tcggcgagac
     5341 tgccccagcg cgccgtgaga aacgcgagca gcgtgttgaa ggcg
//
LOCUS       DASUJG010000152         2451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1633816, whole genome shotgun sequence.
ACCESSION   DASUJG010000152 DASUJG010000000
VERSION     DASUJG010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633816"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="VFX38_03205"
     CDS             complement(<1..813)
                     /locus_tag="VFX38_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX5313901.1"
                     /translation="MKLLLRFAIFSIALCAAAAAVARAPQTSSFTMTQVLGYPYPENL
                     VAAKQGDRIAWTLDERGVRNVWVASGPDFTPRELTHYGDDDGQELTQLQFSPDGKYLV
                     FVRGGDHDANWPESPPPDPDSSTTEPEVTIWAAVLPDGKPIQVASGDEPAISSTNRLA
                     YVKDHQVWSAALDGKGKPARLFFDRGDDGSLRWSPDGSLLAFVSDRDDHAFIGIFTSK
                     DRPLEYLAPSTHRDFSPRWSPDGSRIAFLRMPGVGGPPQPILKLTPEPWAIWI"
     gene            complement(829..>2451)
                     /locus_tag="VFX38_03210"
     CDS             complement(829..>2451)
                     /locus_tag="VFX38_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5313902.1"
                     /translation="TVLQHKNAVKPTGVRAAMAYTRATPPGIDPMRVPAVLLAAGILA
                     LTLAFAPARADDLDAYVQTTMVQWKVPGIAVAVVKDGQVVLARGYGRRELGKPGKVDA
                     ATLFGIASNSKAFTAAALGTLESAGKLGWDDPVIDYLPGFRLESPYVTRALTVRDLLT
                     HRSGYCDPTFMWLTGGFDSREIVHRLRFQKPQYGLRERFCYNNTMYLAAGEIVPAITG
                     ESWDDYVAAHFFAPLGMKRSTSRMAALAKDADVAVPHAEIDGKVVPIPRMDTDSMAPV
                     GGIKSSVADLSHWILMLLADGKYQGRTILDPGIVRTMEAPQMLIARDSEIGEWALAQT
                     PNSQFLAYGLGFFVEDYGGHKLVWHAGDIDGMASALAMIPDEHLGVVVLSNMNQNRAP
                     EAIVFHVLAGYLGLPARDVSGALQKLVKSEEAPGKKLDARLAAARDPAAKPSLPLADY
                     AGRYRNQFVGDVTVAYRNDALVLAFGNPAFTATLVPGAHDTFKAPWRDRVYGDSWVSF
                     DLDALGHAEALRFAGIPYEFTRVTPAAAPEGQ"
BASE COUNT          430 a          748 c          855 g          418 t
ORIGIN      
        1 gatccagatc gcccagggct cgggcgtgag tttcagaatc ggctgcggcg gaccgcccac
       61 gccgggcatg cgcaggaagg cgattcggct gccgtccggc gaccagcgcg gcgagaaatc
      121 gcgatgggtg gagggcgcga gatattcgag cggccgatcc ttcgaagtga agatgccgat
      181 gaaggcgtga tcgtcgcgat cggagacgaa ggcgagaaga ctgccatcgg gcgaccagcg
      241 aagcgagccg tcgtcgccgc gatcgaagaa gaggcgcgcg ggtttgccct tgccgtcgag
      301 cgcggcgctc cagacttgat gatccttcac atacgcgagc cgattggtac tggaaatcgc
      361 gggttcgtcg ccgcttgcga cctgaatcgg tttgccgtcg ggaagcaccg cagcccagat
      421 cgtcacctcg ggctcggttg tggacgaatc cggatcgggc ggcggactct ccggccagtt
      481 ggcgtcgtga tcgccgccgc gcacgaagac gaggtacttg ccgtcgggcg agaattgcag
      541 ttgcgtgagt tcctgtccat cgtcgtcgcc gtaatgagtg agttcgcgcg gcgtgaagtc
      601 gggcccggag gcgacccaga cgttgcgcac accgcgttcg tcgagcgtcc aggcgatgcg
      661 atcgccctgt ttcgcggcga ccaggttttc gggataggga tagccgagca cctgcgtcat
      721 cgtgaacgat gaggtctgcg gtgcgcgcgc aactgcggcg gccgcggcgc agagcgcgat
      781 tgaaaagatg gcgaaacgca acaaaagttt catcgcggaa tctccggtct attggccttc
      841 aggtgcggcc gcaggcgtga cgcgggtgaa ctcgtaggga atcccggcaa aacgcagagc
      901 ttccgcatga ccgagagcat cgaggtcgaa gctgacccag gaatcgccgt agacgcgatc
      961 gcgccacggc gccttgaagg tatcgtgcgc cccgggaacg agcgtcgcgg tgaaggccgg
     1021 attgccgaag gcgagcacca gcgcatcgtt ccggtacgcg acggtgacgt cgccgacgaa
     1081 ttggttgcga tagcgcccgg cgtagtccgc gagcggaagc gacggcttcg ccgcaggatc
     1141 gcgcgccgcc gcgagccttg cgtcgagttt cttcccgggc gcctcctcgc tcttcacgag
     1201 cttctgcaag gcgccgctca cgtcgcgcgc cggaagcccg agatagccgg cgagtacatg
     1261 gaacacgatc gcttcgggcg cacggttctg attcatgttg ctgagcacga cgacccccag
     1321 atgctcgtcg ggaatcatcg cgagcgccga ggccatgccg tcgatgtcgc cggcatgcca
     1381 gacgagtttg tgtccgccgt aatcctcgac gaagaatccg agaccgtagg cgagaaattg
     1441 actgttcgga gtctgcgcga gcgcccactc gccgatttcg ctgtcgcgtg caatcagcat
     1501 ctgcggcgct tccatcgtcc ggacgatgcc ggggtcgagg atcgtgcggc cctgatattt
     1561 gccgtcggcg agaagcatga ggatccagtg cgacaggtcg gcgaccgacg atttgatccc
     1621 gccgaccggc gccatcgaat cggtgtccat gcgcgggatc ggcacgacct tgccgtcgat
     1681 ctcggcgtgc ggcacggcaa catccgcatc cttcgcgagc gcggccatgc gcgaagtgct
     1741 gcgtttcatg ccgagcggag cgaagaaatg cgcggcgacg tagtcgtccc agctctcgcc
     1801 cgtaattgcc ggcacgatct cgcccgcggc gagatacatc gtgttgttgt agcagaagcg
     1861 ctcgcgcagc ccgtactgcg gcttctgaaa gcgcagccga tgaacgatct cgcggctgtc
     1921 gaagccgccg gtgagccaca tgaaggtcgg gtcgcaatag ccgctgcgat gcgtgaggag
     1981 atcgcgcacc gtgagcgcgc gggtcacgta cggactttcg agacggaagc cggggagata
     2041 gtcgatgacg ggatcgtccc agccgagttt cccggcgctt tcgagggtgc cgagcgccgc
     2101 ggcggtgaag gccttggagt tggaggcgat gccgaacagc gtggcggcgt ccaccttgcc
     2161 gggcttgccg agttcgcgcc ggccgtagcc gcgggcgagg acgacctggc cgtccttgac
     2221 gacggcgacg gcgattcccg gcaccttcca ttgcaccatc gtcgtctgca cataggcatc
     2281 cagatcgtcc gcccgggccg gcgcgaaggc caatgtcagg gcgaggatgc cggcggcgag
     2341 caggacggcg gggactctca tcgggtcgat tccgggcggc gtcgcccgag tgtacgccat
     2401 cgccgcgcga actcccgtcg gcttgacggc attcttgtgt tgtagtacag t
//
LOCUS       DASUJG010000153         4474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_410710, whole genome shotgun sequence.
ACCESSION   DASUJG010000153 DASUJG010000000
VERSION     DASUJG010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410710"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="VFX38_03215"
     CDS             <1..961
                     /locus_tag="VFX38_03215"
                     /inference="COORDINATES: protein motif:HMM:NF037202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type ISP restriction/modification enzyme"
                     /protein_id="HEX5313903.1"
                     /translation="PRHEVMRHLIPDRLALIVSRQSNASNWANVHIATGVVDHCLVSN
                     RTSEIGSVFPLWLYPREQADLLDDAPREKADLLDEAPREKTANLAPEFVAALSEAVGH
                     EPTPEDTLAYIYALLYAPSYRQRYADFLKRDFPRVPLTTDRKLFAKLVETGRELIALH
                     TMQTALPSITGFPVAGTNQVGKVRFALPSPGGRGVGGEGPESAQHSKSAEPSSDSSAC
                     LPPKGEGTPPQPSPARGGSEGGGRVYINAEQYFEGVPQAVWDLHIGGYRVAEKWLKDR
                     KDRALSYDDLTHYQNVVAALARTLELQSELDAAIEKAGGWPIT"
     gene            complement(1132..1905)
                     /locus_tag="VFX38_03220"
     CDS             complement(1132..1905)
                     /locus_tag="VFX38_03220"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX5313904.1"
                     /translation="MVRNLPPPAGKESTAIAESRRRFELAFGISFWFTATLWWIELVQ
                     KIGGWNLSGLGIEPHVLFGLIGVVTAPLLHGSWEHLLSNTLPLVILGTALLYGYQRAS
                     RYVVPLVWLGSGLGVWFTGQPAFYVGASGVVMGFIAFLLVAGALRRDRTSIAITCVVA
                     FLYGTALVEIVPQGEPHIAYVAHAWGGAIGLLCAIVLFRLDPIPPRKRFHWQDEPEDF
                     DDPVIGDLWREPPPAKVAPPPPPPRLPAPTDRDDPPRLH"
     gene            2008..2451
                     /locus_tag="VFX38_03225"
     CDS             2008..2451
                     /locus_tag="VFX38_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313905.1"
                     /translation="MSAEESGAPAVAGARGLALLASTRRWAIVVGLVFAIFAALMIAS
                     YLARFFGDRPANLSPEIWKFSRIALLVLAVLVLLLQGALSCFALQYGRRIGHSLARMD
                     PVLLETALWWQRRFWTLQGALIMAVLILWGLAFVVGIFAVFLHLA"
     gene            2458..2916
                     /locus_tag="VFX38_03230"
     CDS             2458..2916
                     /locus_tag="VFX38_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313906.1"
                     /translation="METQDDPAPEAIALSAGALRALLKTRFWVLFLGVVGVIVAIVGG
                     IMLLVGAVTLGRDLPHGSFLIGEGAVLLIVFALFSIWQLRYSGGLRQLKAAPGMTNAD
                     IEELFARQRGLWRVTGILMLALIAVMILGAVLAASGGVPLQRLFSALSGG"
     gene            2995..4263
                     /locus_tag="VFX38_03235"
     CDS             2995..4263
                     /locus_tag="VFX38_03235"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5313907.1"
                     /translation="MTPSEENTTSRSSRGQRIANLVSWRIAIMAFLGGIGGGVVFPIL
                     PALGQRMGLSAFMIGVILAANRIARLGVNPLTGALTDRYGGRGVIATGLFIEGFGTLG
                     YIAALHFALPAAWFLAGRLIWGVGSSLLFVGAIAAVLTLSESRDRGRFVARARSAVSL
                     GVPGGLVLGGIVADLVSADAAFGVAMALSIAAGFAALAGIPPRAPHTRRRMREARPQG
                     TRDWLQLLRQRRLAGIWLYAALISFAVQGLLLATLVLLVEQRNTFIADFGAEGSAGLL
                     MAILMLAYAGTSVAIGRRLDLLGRRTVLIVPTVVILIAGFALLAWAPSLAWVLVALIV
                     TGVGTGAAIIPILTLLGDLVPPERRGRATAIYQVAADAGGTLGPILGLVLGVRFGFLF
                     LYGGLALLFVLSLPLALALVRAERSASGPE"
BASE COUNT          715 a         1486 c         1457 g          816 t
ORIGIN      
        1 cccgcgtcac gaagttatgc ggcatctaat accagaccga ctggctttga tcgtaagccg
       61 ccagtcgaat gcctcaaatt gggctaacgt gcacatagcg accggcgtcg ttgatcactg
      121 cttggtttcc aacaggacga gcgagatcgg ctccgttttt ccgttgtggc tttatccgcg
      181 cgaacaggcc gatcttctgg acgacgcacc gcgcgaaaag gccgatctgc tggacgaggc
      241 gccgcgggaa aagaccgcca acctcgcgcc ggagttcgtc gcggcgctga gcgaagccgt
      301 cggccatgag cccacgcccg aagacacgct ggcgtacatt tacgccctgc tctacgcgcc
      361 gtcgtatcgc cagcgctacg cggatttcct gaagcgcgac tttccgcgcg tgccgctgac
      421 cacggatcgc aagctcttcg caaaactcgt cgaaacgggc cgggaactga tcgcgctgca
      481 cacgatgcag actgcgttgc cgagcatcac cggctttccg gttgcgggaa ccaaccaagt
      541 tgggaaagtc cggttcgcac tcccctctcc cggcgggaga ggggtcgggg gtgagggtcc
      601 ggaatcggcg caacactcga aatccgccga accctcatcc gactcttcgg cgtgccttcc
      661 gccgaagggc gaaggaaccc caccccaacc ctcccctgca aggggaggga gtgaaggcgg
      721 tggccgcgtg tacatcaacg cggaacaata cttcgagggc gtgccgcagg cggtgtggga
      781 tctgcacatc ggcggctacc gcgtcgccga aaagtggctc aaggatcgca aggaccgcgc
      841 tctgagttac gacgacctca cgcattacca gaacgtcgtc gccgccctcg cccgcaccct
      901 cgaactgcaa tcggaactcg atgccgcgat cgaaaaggca ggaggctggc cgatcacctg
      961 aaacaaatcc cgtctacgct ttgcccgcaa cgcttcgctt atgcgggcct gcaggagcga
     1021 cgtaggccgg gataagccgc aaggcggcgt tcccggcatc ccatcttgtc ttgccggcaa
     1081 cgctgcgctt atgcgggcct gcaagaactg cgctggtgcg ggcctgccga gtcagtgaag
     1141 gcgcggcgga tcgtcgcgat cggtcggcgc gggcagtcgt ggcggcggtg gcggcggcgc
     1201 aactttcgcg ggcggcggtt cgcgccagag atcgccgatg acgggatcgt cgaaatcctc
     1261 cggttcgtcc tgccagtgga agcgcttgcg cggcggaatc ggatcgagtc gaaacaagac
     1321 gatcgcgcac agcaacccga tcgcgccgcc ccaggcatgg gcgacgtagg cgatgtgcgg
     1381 ctcgccctgg ggaacgattt cgacgagcgc cgtgccgtag aggaaggcga cgacacaggt
     1441 gatcgcgatc gaggtgcggt cgcgacgcaa cgcaccggcg acgagcagaa acgcgatgaa
     1501 ccccatgacg accccgctcg cgccgacgta aaacgcaggc tggccggtga accatacgcc
     1561 gagccccgag cccagccaca ccagcggcac gacgtagcgg ctcgcgcgct gataaccgta
     1621 cagaagcgcc gttccgagaa ttacgagcgg cagggtattc gagagcagat gttcccagga
     1681 gccgtgaaga agcggcgccg tcacgacgcc gatgagaccg aagagaacgt gcggctcgat
     1741 gccgaggcca ctcaaattcc agccgccgat cttctgcacg agttcgatcc accagagcgt
     1801 cgccgtgaac cagaaggaaa tgccgaaggc gagttcgaaa cggcgccgcg actcggcgat
     1861 cgcggtgctt tccttcccgg cgggcggcgg cagattcctg accatcgaag ggcatcttag
     1921 tacgcggcgg acgcggccgg ccgcactccc cttcctgccg cccgcggtgg gcctataatc
     1981 tgctgctcgc gcggcacggg agggaatatg agcgcggaag aatccggagc gcctgcggtc
     2041 gcgggagcga gggggctggc gctccttgcg agcacgcggc gctgggcgat cgtggtcggt
     2101 ctcgtgttcg cgatcttcgc cgccctgatg atcgcgagtt atctcgcgag attcttcggg
     2161 gatcgtcccg ccaatctgtc tcccgaaatc tggaagttca gccgcatcgc cttgctggtg
     2221 ctggccgtac tggtgttgct gctgcaaggc gcgctgagct gctttgcgct ccagtacggc
     2281 cggcggatcg gccacagtct tgcgcgaatg gatcccgtgc tgctggaaac ggcgttatgg
     2341 tggcagcgcc gtttctggac cctccaaggt gcgctcatca tggcggtcct gattctctgg
     2401 gggctcgcat ttgtcgtcgg cattttcgcc gtcttcctgc acctcgcctg aatcgcgatg
     2461 gaaacgcagg acgatcccgc accggaggca atcgcgctca gcgccggcgc tctgcgagcg
     2521 cttctcaaga cgcgcttctg ggtgcttttt ctcggcgtgg tcggcgtgat cgtcgccatc
     2581 gtcgggggaa tcatgctgct cgtcggcgcg gttaccctcg gtcgggatct tccccacggc
     2641 tccttcctga tcggcgaagg cgcagtcctg ctgatcgtct ttgccctgtt ttcgatctgg
     2701 caactgcgct attcgggggg gttacgccaa ctgaaggcgg cacccggcat gacgaacgcc
     2761 gacatcgaag aacttttcgc gcgccagcgc gggctgtggc gggtcacggg gatcctgatg
     2821 ctggccctga tcgcagtcat gattctcggg gccgtcctcg cggcctccgg cggagtcccg
     2881 ttgcagcgtc tcttttccgc cctctccggc ggctgagcgc gaagtgcctt cgcgggcgtc
     2941 gcggccgcca gcgccatccg ctactctgat gttttgcttc cgggatgcgg aagcgtgacg
     3001 ccctccgaag aaaacacgac atcgcgttcg agccgcggcc agcggatcgc caacctcgtg
     3061 agctggcgca tcgcgatcat ggcctttctc ggcggcatcg gcggcggagt cgtgtttccg
     3121 atcctcccgg ctctcggcca gcgcatggga ctgtccgcgt tcatgatcgg cgtcattctc
     3181 gcggccaacc gaatcgcccg tctcggcgtg aacccgctca ccggcgcgct caccgatcgc
     3241 tacggcgggc gcggcgtgat cgcgacggga cttttcatcg aaggcttcgg caccctcggc
     3301 tacatcgcgg cgctgcactt cgcgctgccc gcggcctggt ttctcgccgg gcgcctgatc
     3361 tggggtgtgg gctcctcgct cctgttcgtc ggcgcgatcg cggccgtgct gacgctgagc
     3421 gaaagccgcg atcgcggccg cttcgtcgcc cgcgcgcgga gcgccgtcag tctcggcgtt
     3481 cccggcggac tggtccttgg cggcatcgtt gccgatctcg tttccgccga cgccgccttc
     3541 ggcgttgcga tggcgctcag catcgcggcg ggattcgcgg cgctcgcggg cattccgccg
     3601 cgcgcgcctc acacgagaag gcggatgcgc gaagcgcgtc cgcagggcac gcgggattgg
     3661 ctgcaactgt tgcgccagcg gcgactcgcg ggaatctggc tgtatgcggc gctcatctcc
     3721 ttcgcggtgc aaggtcttct gctcgcgacc ctcgtgctgc ttgtcgagca gcgcaacacc
     3781 ttcatcgccg acttcggggc cgagggcagc gcggggctgc tcatggcgat tctcatgctc
     3841 gcctacgccg gcacctcggt cgcgatcggc cgccggctcg atcttctcgg acgacgtacc
     3901 gttctgatcg tgccgactgt cgtcatcctg atcgccggtt tcgcgctgct cgcctgggcg
     3961 ccgagcctcg cctgggtgct cgtcgccctg atcgtcaccg gcgtcggcac cggcgccgcg
     4021 atcattccga tcctcacgct cctcggcgat ctcgtgccac ccgaacggcg gggacgcgca
     4081 accgcgatct atcaggtcgc cgcggacgcc ggcggaacgc tcggaccgat tctcggcctc
     4141 gtcctcggcg tgcgcttcgg gttcctcttc ctctacggcg ggctcgcgct gcttttcgtg
     4201 ctgagcctgc cgctcgcgct ggcactcgtg cgcgccgagc ggagcgcctc cggccctgag
     4261 tagaatggca gacgagaaca tttcgccgtc ggcaacgcga cgcggtgatg ttcccatgat
     4321 cctcaggcag gagagcagca tgaacgcata tcccgacgag aatgaacacg gtctcgaacg
     4381 gcgcggcggc aaaaccgggt tcttggtgac cgtcacttcc tatgtcagcc tgattcccta
     4441 catcgggctc gccatatggc tgtggaccca caag
//
LOCUS       DASUJG010000154         2368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1145448, whole genome shotgun sequence.
ACCESSION   DASUJG010000154 DASUJG010000000
VERSION     DASUJG010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145448"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1866)
                     /gene="rpoB"
                     /locus_tag="VFX38_03240"
     CDS             complement(<1..1866)
                     /gene="rpoB"
                     /locus_tag="VFX38_03240"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628201.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX5313908.1"
                     /translation="MAFPVTEKKRVRKDFGKSTSVLPVPYLLAMQLDSYRQFLQSDKT
                     VEEREATGLHGALVSVFPIESYSGNAALVYRGYTIGSPAFDVRECQIRGLTYAAPLKA
                     TLDLVFYEKESGGGRHQKGDIKEQEVYLGELPLMTANGTFVVNGTERVVVSQLHRSPG
                     VFFDHDRGKSHSSGKLLYAARIIPYRGSWLDFEFDPKDCLFVRIDRRRKLPVTILLRA
                     LDYTNEQMLAMFFEHNVIHFRDGELHFDLVAERMKGELAGFDIVVKGKTVVEAGRRIT
                     ARHVRELEKAGVSSIVVPREYVTGQVIAHDVVDETTGELLARANEVIDAAVLERLVDK
                     GVGEIRTLAINDLDRGSFLSDTLRLDATTTRLEALVEIYRMMRPGEPPTKEAAEQLFA
                     NLFFNSDRYDLSAVGRMKFNSRLGREDRSGPGVLAPEDILDVIKALIDIRNGHGETDD
                     IDHLGNRRVRSVGEMAENAFRIGLVRVERAVRERLAIAESEGLMPQDLINAKPVAAAM
                     KEFFGSSQLSQFMDQNNPISEVTHKRRVSALGPGGLTRERAGFEVRDVHATHYGRICP
                     IETPEGPNIGLINSLSVFARTNEFGFLETPYRKIVGGRVSDEIQYLSAIEEGQYHI"
     gene            complement(2002..>2368)
                     /gene="rplL"
                     /locus_tag="VFX38_03245"
     CDS             complement(2002..>2368)
                     /gene="rplL"
                     /locus_tag="VFX38_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX5313909.1"
                     /translation="EDILETISNMTVMEVVDLISAMEEKFGVSAAAAVAAAPAAGAGA
                     PAAAAEEKTEFDVVLQSFGANKVAVIKAVRAITGLGLKEAKDTVEGAPATLKEGVAKA
                     DAEKMKKDLEEAGATVEIK"
BASE COUNT          415 a          778 c          724 g          451 t
ORIGIN      
        1 gatgtgatac tgcccctctt cgattgccga aaggtactga atctcgtcac tcacccgccc
       61 gccgacgatc ttgcgatagg gagtttcgag aaacccgaat tcgttcgtgc gcgcaaagac
      121 cgacagtgaa ttgatgaggc cgatgttcgg cccttccggg gtctcgatcg ggcagattct
      181 gccgtagtgg gtcgcgtgca cgtcgcgcac ctcgaagccc gcgcgctcgc gggtgagccc
      241 gccgggcccc agggccgaga ctcgccgctt gtgcgtcacc tcggagatcg ggttgttctg
      301 atccatgaac tggctgagct ggctcgaacc gaaaaactcc ttcatcgccg ccgccaccgg
      361 cttggcgttg atgagatcct gcggcatgag cccctcggat tctgcgatcg cgagccgctc
      421 gcgcacggcc cgctccacgc gaacgagtcc gatgcgaaag gcgttctcgg ccatctcgcc
      481 gacgctgcgt acgcggcggt tgcccagatg atcgatgtca tcggtctcgc cgtgcccgtt
      541 gcggatgtcg atgagcgcct tgatcacgtc gagaatgtcc tcgggcgcga gaacgccggg
      601 gccgcttcga tcttcgcgcc cgagcctgct gttgaacttc atccgcccga ccgcggacag
      661 atcataacga tcggaattga aaaagagatt cgcgaacaac tgctcggccg cttccttcgt
      721 cggcggctct cccgggcgca tcatgcggta aatctcgacg agcgcctcca aacgggtcgt
      781 cgtcgcatcg aggcgcaggg tgtcggaaag gaacgaaccg cgatcgagat cgttgatcgc
      841 gagcgtgcgg atctcaccga cgcccttgtc gacgagccgc tcgagcaccg ccgcgtcgat
      901 gacctcgttc gcgcgggcga gaagctcgcc cgtggtctcg tccaccacgt catgggcgat
      961 tacctgcccc gtcacgtatt cgcgcggaac gacgatgctc gacaccccgg ccttctccaa
     1021 ttcgcgtaca tgacgcgccg tgatgcgccg cccggcctcg accacggtct tgcccttgac
     1081 cacgatgtca aagccggcga gttcgccctt catgcgttcg gccacgagat cgaaatgcag
     1141 ctcgccgtcg cggaaatgaa tcacgttgtg ctcgaagaac atggcgagca tctgctcgtt
     1201 ggtgtagtcg agcgcgcgca ggagaatcgt taccggaagc ttgcgccgcc ggtcgatacg
     1261 cacgaacagg caatccttcg ggtcgaactc gaagtcgagc caggagccgc ggtagggaat
     1321 gatgcgcgcc gcgtagagga gctttcccga ggagtggctc ttgccgcgat cgtgatcgaa
     1381 gaagactccg ggcgagcggt gcaactggga gacgacgacg cgctcggtgc cattgacgac
     1441 aaaggttccg ttcgccgtca tcaggggaag ctcgccgaga tacacctcct gctccttgat
     1501 gtcgcccttc tgatgacggc caccgccgct ctccttctcg tagaagacca gatcgagcgt
     1561 cgccttgagc ggcgcggcat aggtcagtcc gcgaatctgg cattcgcgca cgtcgaacgc
     1621 cggagagccg atcgtgtagc cgcgatagac gagggcggcg ttgcccgagt agctctcgat
     1681 cggaaacacc gagaccagag cgccgtgcaa acctgtcgcc tcgcgctcct caaccgtctt
     1741 gtcggattgc agaaactggc gataggagtc aagctgcatc gcgagcaggt agggcaccgg
     1801 cagaacgctg gtacttttgc cgaaatcctt gcgcacacgc tttttttcag tcaccggaaa
     1861 tgccatgggg aatcacctcg ccattcgctg ctgtcggttc gccggacggc cacagccgcc
     1921 ggggaaaatg cgcgcgagca gaccggcgct caggccggtc tgccgcactg gttcggttct
     1981 gacacgggtc gggaggcgag actacttgat ctcgaccgtc gcaccggcct cctcgaggtc
     2041 ctttttcatc ttttccgcgt ccgccttggc gacgccttcc ttgagcgtcg cgggcgcgcc
     2101 ctcgaccgtg tccttggcct ccttcaggcc gaggccggtg atcgcgcgta cggccttgat
     2161 caccgcgacc ttgttcgcgc cgaagctttg cagcacgacg tcgaactcgg tcttctcctc
     2221 ggccgcggcc gccggggctc ccgcccccgc cgccggcgcc gcagcaactg ccgcggcggc
     2281 cgaaacgcca aacttttctt ccatcgcgga gatgaggtcg acgacctcca tcaccgtcat
     2341 gttggaaatc gtctccagaa tgtcttcc
//
LOCUS       DASUJG010000155         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_290226, whole genome shotgun sequence.
ACCESSION   DASUJG010000155 DASUJG010000000
VERSION     DASUJG010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290226"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VFX38_03250"
     CDS             <1..756
                     /locus_tag="VFX38_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012969673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR domain-containing protein"
                     /protein_id="HEX5313910.1"
                     /translation="HENEIAQSEAATGERFAKYWMHNGFVEVGAEKMAKSLGNFITIR
                     ALLARWPGETLRYFLLGSHYRSPLSYTEERIAEAHAALSRLYLALRGLGTTVPGNGAS
                     EIVPSRIDAAPDGARGREEPGSGGNYRERFHAAMSDDFNTPQAIAVLHDLASEINRSR
                     EAGEDARAQAAELRRLAATLGLLQADADAFLRRGSGAAGLSVQAIEGLIEERRAARAA
                     RDFRRADEIRQELAAAGIVLEDTPEGTLWRRSG"
     gene            complement(760..2004)
                     /locus_tag="VFX38_03255"
     CDS             complement(760..2004)
                     /locus_tag="VFX38_03255"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5313911.1"
                     /translation="MATLIGRLFGLLLGITILLTGMGLLGSLLGLRATEAHFPGWELG
                     LVMSGYFAGFIVGSFLCPVLIRRVGPIRSFAAFAAIVASATLAHALAVNPWSWLALRL
                     VTGTAMVGLYMVTESWLNASLPRPYRVQIFSFYAMLTLVAMAAGQFLFFAVPANGTAL
                     FLVAGMLIVAGLIPVTATRLDQPPAVSAPRLPPLDLLRRAPFAVAGTFLSGLTTGAFW
                     GLGAAFAARLGFSPGQIGLFVACTVAGGIVAQWPLGRLSDRVGRPPVMIAAAALGCVF
                     ALLLAAATQGPEWPLLIFGFGFGAMALCLYALAVAHMNDVLEPPEVLSATQGLLLATG
                     VGSMLGPLIAGMMFDAAGARGLPLYLAAVLAIFALSGLLYSLRRVMPRPREQSEFVPM
                     VRTSQVALEIVEESRGPQPGGP"
     gene            complement(2023..>2220)
                     /locus_tag="VFX38_03260"
     CDS             complement(2023..>2220)
                     /locus_tag="VFX38_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313912.1"
                     /translation="LAGDVDFAAARERAAWITPVPGGVGPMTVAMLLENTLEAWSRRR
                     ATPGGADASASAADGVQVSSA"
BASE COUNT          431 a          733 c          782 g          274 t
ORIGIN      
        1 cacgagaatg aaatcgcgca gtcggaggcc gcgacgggcg aacgcttcgc gaagtactgg
       61 atgcacaacg gtttcgtcga agtcggtgcc gagaagatgg ccaaatcact cggaaacttc
      121 atcaccatcc gcgcgttgct cgcgcgctgg ccgggcgaaa ccttgcgcta cttcctcctc
      181 ggcagtcact accgaagtcc cttgtcctat accgaagagc ggatcgccga ggcgcatgcc
      241 gcgctttcgc ggctctatct cgcgctccgg ggtctcggca caactgttcc cgggaatggt
      301 gcttcggaaa tcgttccttc ccgaatcgac gccgcgccgg acggggcgcg ggggcgtgaa
      361 gagccggggt ctggcgggaa ttatcgcgag cgctttcacg ccgccatgag cgatgacttc
      421 aacaccccgc aggcgatcgc ggttctccac gatctcgcga gcgaaatcaa tcgcagccgc
      481 gaagcgggcg aagatgcgcg ggcgcaggca gcggagcttc gccggctcgc ggccaccctg
      541 gggctgctgc aagccgacgc cgatgcattt ctccgccggg gaagcggagc cgcgggcctc
      601 tcggtgcagg cgatcgaagg gctcatcgag gagcgtcgtg ccgcccgcgc cgcccgcgat
      661 ttccgccgcg ccgacgaaat ccgccaggag ctcgccgcgg ccggaatcgt tctcgaagat
      721 acgcccgagg ggacgctctg gcggcgcagc ggctgagtct caggggccac ccggctgcgg
      781 cccgcgcgac tcttcgacaa tttcgagagc gacctgggag gtgcgcacca tcgggacgaa
      841 ctcgctctgc tcgcgaggac gcggcatgac ccgccgcaga ctgtacagga gtccgctgag
      901 cgcgaagatc gcgagcacgg cggcgagata aagcgggaga ccacgggcgc ccgcggcatc
      961 gaacatcatg cccgcgatca gcggaccgag catcgagccg acgccggtcg caagcagcag
     1021 gccttgcgtc gcagacagaa cttcgggggg ttcgagcacg tcgttcatat gggcgaccgc
     1081 aagcgcgtag agacacagcg ccatcgcgcc gaaaccgaag ccgaaaatga gcagcggcca
     1141 ctcgggtccc tgcgtggccg cggcgaggag cagcgcaaag acacagccga gcgcggccgc
     1201 agcaatcatg actggcggac ggccgacccg atccgacaga cggccgagcg gccactgcgc
     1261 gacgatgccg ccggcgaccg tgcaggcgac gaacaggccg atctggccgg gagaaaagcc
     1321 gagccgcgcc gcaaaggcgg cgccgagtcc ccagaacgcg ccggtggtga gaccggacag
     1381 aaaggtgccg gcgacggcaa agggagcgcg ccggagcaga tccagcggcg gcagtcgcgg
     1441 tgcgctcacg gccggcggtt gatcgagacg cgtcgcggtg acgggaatca ggccggcgac
     1501 gatcagcatt cccgcgacca ggaacagcgc cgtgccgttg gccggaacgg cgaagaacag
     1561 aaattgtccc gccgccatcg caaccagcgt gagcatcgcg tagaagctga agatctgcac
     1621 ccgatagggt cgcggcagac tcgcgttcaa ccagctttcg gtgaccatgt agagcccgac
     1681 catcgcggtg ccggtgacga ggcgcagcgc gagccaggac cagggattga cggcgagggc
     1741 gtgggcgagc gtcgcgctcg cgacgatcgc ggcgaaggcg gcaaaactgc ggatcggacc
     1801 gacccggcgg atgagtaccg gacagagaaa gctgccgacg atgaaacccg caaaatatcc
     1861 ggacatcacc agccccagct cccagccggg aaaatgcgct tcggtcgcgc gcaggccgag
     1921 aagcgagccg agcagcccca ttcccgtgag aaggatcgtg atgccgagaa gcaggccgaa
     1981 gagccggccg atcagggtgg ccataggcgc gccgcgatgc gctcaggcgc tcgaaacctg
     2041 cacgccgtcc gcggcggacg ccgaagcgtc ggcgccgccc ggcgtcgcgc ggcgtcggct
     2101 ccaggcttcg agggtgtttt cgagcagcat cgcgaccgtc atcggcccga cgccgccggg
     2161 aacgggcgtg atccaggccg cgcgttcgcg cgccgcggcg aagtccacgt cgccggcgag
//
LOCUS       DASUJG010000156         2381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_534875, whole genome shotgun sequence.
ACCESSION   DASUJG010000156 DASUJG010000000
VERSION     DASUJG010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534875"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="VFX38_03265"
     CDS             complement(<1..929)
                     /locus_tag="VFX38_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313913.1"
                     /translation="MNTTSMPNAALVSPDSTPTAASGDALLLDLDPAFTRDAVGRREF
                     AFRHHVAKHPLLGLEALAELADRVPPAAVERHAASQPLLVPGGAEDFPGSASETVRNI
                     ERDRHWLVLKNVEQIPEYSRLLDDILDEAAPLLPRREEGMGRREAYLFMSAPRAVTPV
                     HFDPEHNFLLQIRGSKEVCVGRFADRDSRLAELDRYYNGGHRNLVAIPSRSSVFPLTA
                     GDGVYLYPWAPHWVHNGPEVSVSLSITFRSRRSERFEFASLFNHRLRRYGMHPRPAGE
                     SDLRDRGKAAAVSLSGWLHRHGRRQRGDFDFSG"
     gene            complement(926..1984)
                     /locus_tag="VFX38_03270"
     CDS             complement(926..1984)
                     /locus_tag="VFX38_03270"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5313914.1"
                     /translation="MVAEWDALARRLCAPPQQRPGWLEAWWQAFGTDRLRLYALKCEG
                     RLAAVLPMIRRGDDFVGAANVHTPGFAMTTESPAAADALAEMVYSDAPRRVSLVDLEP
                     GGETFRAFLHAAHVAGYSVLIRPWCCSPYLAVDGSWEVYESRLGKNLVRNLRRRSNQI
                     ALRGDVRFQFSDGSTDLDALLAEAFRVEASGWKGRHGTAIAADPRTRKFYTALARWAT
                     SEGTFRLAFLRLDGRAIAMDYRIIQGSVCHSLKAGYDEEFHRYSPGTLLLHAVLRRCF
                     AAPGISRIEFYGNGEDYKFLWTRTTAERLRLEAFAPTLRGHSRRVAFVYGRPLATRLA
                     AAGRRLFPINFHLAGARS"
     gene            complement(2184..>2381)
                     /locus_tag="VFX38_03275"
     CDS             complement(2184..>2381)
                     /locus_tag="VFX38_03275"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEX5313915.1"
                     /translation="TLVPVIFGFGAVMNVAFCFALIPPLGAVGAATAAAAAPLSWMCL
                     AVYTVRRKLRLNPTILRFLDV"
BASE COUNT          454 a          755 c          802 g          370 t
ORIGIN      
        1 tatccgctga agtcgaagtc gccgcgctgg cgccggccgt gccggtgcag ccagcccgaa
       61 agcgacacgg cggccgcctt gccgcggtcg cgcagatcgg actcgcccgc ggggcgcggg
      121 tgcatgccgt agcggcgcag gcgatgattg aaaaggctcg cgaactcgaa gcgctcgctg
      181 cgccggctgc gaaacgtgat cgaaagcgac accgagacct ccgggccgtt gtggacccaa
      241 tgcggcgccc acggatagag atagacgccg tcgccggcgg tcaggggaaa cacgctcgag
      301 cgcgagggaa tcgcgacgag gttgcgatgg ccgccgttgt agtagcggtc gagttcggcg
      361 agacgcgaat cgcggtccgc aaagcgcccg acgcatacct ccttcgagcc gcggatctga
      421 agaagaaaat tgtgctcggg gtcgaagtgc accggagtga ccgcccgcgg cgcggacatg
      481 aacagatacg cctcgcgccg tcccatgccc tcttcccggc gcggcagcaa cggcgccgcc
      541 tcgtccagga tgtcgtcgag aaggcggctg tattccggaa tctgctcgac gttcttcagc
      601 accagccagt gccggtcgcg ttcgatgttg cgcaccgttt ccgaggcgga gccgggaaaa
      661 tcctcggcgc ctcccggaac gagcagcggt tgggaagcgg cgtggcgctc gacggcggcg
      721 ggcggaactc tgtcggcaag ttccgcgagc gcttcgaggc cgagaagcgg atgcttcgcg
      781 acgtgatggc ggaaggcgaa ttcgcgtcgg ccgacggcat cgcgcgtgaa tgccggatcg
      841 agatcgagaa gcaaggcatc gccgctcgcc gcggtcggcg tggaatccgg cgagaccagc
      901 gccgcatttg gcatggacgt ggtgttcatg atcgtgctcc tgcaaggtga aaattgatgg
      961 ggaaaagcct cctgcccgcc gcggcgagac gagtcgcgag cggacgtcca tagacgaagg
     1021 cgacgcgtcg cgaatggcct cgcagcgtcg gcgcgaaggc ctcgagccga agtcgttccg
     1081 cggtcgttcg ggtccacaga aacttgtagt cctcgccgtt gccgtagaat tcgattcggg
     1141 aaatgcccgg ggccgcgaag caacggcgaa gcaccgcgtg cagcagcagc gtccccggcg
     1201 agtagcggtg aaattcctcg tcgtagcctg ccttgagcga atggcatacg ctgccctgaa
     1261 taatgcggta atccatcgca atggcgcgcc cgtccagacg caggaaggcg agacggaacg
     1321 ttccctctga agtcgcccat cgtgcgagcg ccgtgtagaa cttccgcgtg cgcggatcag
     1381 ccgcgatggc ggtgccatgc cgtcccttcc acccggaagc ttccactcga aaggcttcgg
     1441 cgagcagcgc gtcgagatcg gtgcttccat cggaaaattg gaagcgcaca tcgccgcgaa
     1501 gcgcgatctg attgctgcgg cgacgaagat tgcgaacgag attcttcccg agccgcgact
     1561 catacacctc ccagcttccg tccacggcaa gataaggcga gcagcaccag gggcggatca
     1621 gaacgctata gcctgcgaca tgcgcggcat ggagaaaggc gcgaaaggtc tcgccgcccg
     1681 gctccagatc cacgagagaa acgcgccgtg gcgcgtcgga ataaaccatc tccgcgagcg
     1741 cgtcggcggc ggccgggctt tccgtcgtca tcgcgaatcc cggcgtgtga acgttcgccg
     1801 ctccgacgaa gtcgtcgccg cggcgaatca tcggcagcac cgcggcaagc cttccttcgc
     1861 atttgagagc gtacaggcgg agcctgtccg ttccgaacgc ctgccaccac gcctcgagcc
     1921 agccgggtcg ttgctgcggc ggcgcgcaga ggcgtcgcgc cagagcgtcc cactccgcaa
     1981 ccaagtcgtt cgcgccgtcc cagcttcccg cggagacttc cagccgagtg caatgtgcgc
     2041 gaagggttgt cgatcgcggc gataccaatc ccgatgttcg aagttgtcgc gcgggcggcg
     2101 catatgcagc atgatgtgcc cgcgaatcgt cgcgcggcag atttgtcgcc acgtttcgct
     2161 ctggcttcgc gcgcgtcgcg ggactaaaca tcgaggaatc tcagaatggt cggattcaat
     2221 cgcaacttgc ggcgcacggt atagacggcc aggcacatcc aggacaaggg tgcggcggct
     2281 gccgccgtgg ccgcgcccac cgctccaagc ggtgggatca aggcgaaaca gaacgcaacg
     2341 ttcattaccg cgccaaagcc gaagatgacc ggcacgagcg t
//
LOCUS       DASUJG010000157         2282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_169486, whole genome shotgun sequence.
ACCESSION   DASUJG010000157 DASUJG010000000
VERSION     DASUJG010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169486"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..171
                     /locus_tag="VFX38_03280"
     CDS             <1..171
                     /locus_tag="VFX38_03280"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:NF020720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX5313916.1"
                     /translation="RDLAYFLRRGQPVLVPHAPTSGWLRLYDEREQFLGVGEILEDGR
                     VAPRRLVQGLAA"
     gene            386..655
                     /gene="rpsO"
                     /locus_tag="VFX38_03285"
     CDS             386..655
                     /gene="rpsO"
                     /locus_tag="VFX38_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX5313917.1"
                     /translation="MPLNKDEKRRIIDDYGSGDGDTGSAEVQVALLSARIEGLGSHFS
                     THGKDHHSRHGLLKMISRRRRLLDYVKASDLERYQRLIERLGLRR"
     gene            691..>2282
                     /gene="pnp"
                     /locus_tag="VFX38_03290"
     CDS             691..>2282
                     /gene="pnp"
                     /locus_tag="VFX38_03290"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX5313918.1"
                     /translation="MPVIRKSFDFGGKEVTLETGEIARQADGAVLVTMAETSVLVTAV
                     GRRQANAAQDFFPLTVNYQEKTYAAGRIPGGFFRREGRPSEKEVLTSRLIDRPIRPLF
                     PKGFKNEVQVIATVISMNPEVDPDIPSLIGASAALAISGLPWRGPIGACRVGYLNDEF
                     VLNPTISQTAESDLDLVVAGTRDAILMVESEAARLSEKTMIDAVFFGHEGIRHAVEAI
                     ESLRAEAGSKPAWEWTQPQLPAALTEDLKRRYQDAITKAYGITAKSERQAELARLREE
                     ALAQLGTGEAGFAAADVLDAFTKFERGIVRGRIIAGDKRIDGRGPKDVRAITVRVGVL
                     PRTHGSALFTRGETQALVVTTLGTERDAQRIDALAGEHKEPFMLHYNFPPYSVGETGF
                     MGSPKRREIGHGRLAKRGIQAVMPDMTAYPYVIRVVSEVTESNGSSSMATTCGTSLSL
                     MDAGVPIDAPVAGVAMGLIKEADGYAVLTDILGDEDHLGDMDFKVAGTKDGVTALQMD
                     IKIDGITREIMETAMSQAREGRL"
BASE COUNT          411 a          753 c          734 g          384 t
ORIGIN      
        1 cgcgatctcg cctacttttt gcgccgcggt cagccggttc tcgtgccgca cgcgcccacc
       61 agcggctggt tgcgactcta cgacgaaagg gagcaatttc tgggagtcgg ggaaattctc
      121 gaggacgggc gcgttgcccc gcgccgcctt gtccagggcc tcgccgcttg agccgcgacc
      181 cgtcaggccg taaaataggc cggttcgtgg gcttcgggcc gtgatccgct cctccgggga
      241 cggatcgggc gcgatggctc ggcatgattc cggacttctc ccgacccgcc cagacatgcg
      301 agtgtccggc gaagttcctc tgccgcacac gcaggccgcg aacgtatggt ttaccggatt
      361 caccgacagc ggagattgac gagatatgcc actcaacaag gacgagaagc gccgcatcat
      421 cgacgattac ggctcgggcg acggcgacac cggctcggcg gaggtgcagg tcgccctcct
      481 ttctgcccgc atcgaggggc tcggttcgca cttctccacc cacggcaagg atcatcattc
      541 acgccacggc ctgctgaaga tgatcagccg gcgccggcgc cttctggatt acgtcaaggc
      601 ctccgatctc gagcgctacc agcggctgat cgagcgcctc ggcctgcgcc gctgagcggc
      661 atcaccgacc catcttctgc gggagttatc gtgccagtca tcagaaagag tttcgatttc
      721 ggaggcaagg aagtgaccct ggagacgggc gaaatcgccc gccaggccga cggcgccgtg
      781 ctggtgacga tggccgagac ttccgtgctc gtcaccgccg tcggccgccg ccaggcgaac
      841 gccgctcagg atttctttcc cctcaccgtc aattatcagg agaagaccta tgccgccggc
      901 cgcattccgg gcggcttctt ccggcgcgag ggaaggccga gcgaaaagga ggtgctcacc
      961 tcgcggctga tcgaccgccc gatccgtccg ctcttcccga aggggttcaa gaacgaggtg
     1021 caggtcattg cgaccgtgat ttccatgaac ccggaagtgg atcccgacat tccttcgctg
     1081 atcggcgcct ccgcggcgct cgcgatctcg gggttgccgt ggcgcgggcc gatcggcgcc
     1141 tgccgcgtcg gttatctgaa cgacgaattc gtgctcaacc cgacgatttc ccagaccgcc
     1201 gaatccgatt tggacctggt agtcgccggc acccgcgatg ccattctcat ggtcgaatcc
     1261 gaggcggcgc ggctctccga aaagaccatg atcgacgccg tatttttcgg ccacgaaggg
     1321 attcgccatg cggtcgaggc gatcgaatcg ctgcgtgcgg aggcgggcag caagcccgcg
     1381 tgggaatgga cccaaccgca actgccggcg gcactcaccg aagatctcaa gcgccgctat
     1441 caggatgcga tcaccaaggc ctacggcatt acggcgaaga gcgaacgtca ggccgaactc
     1501 gcgcgtctgc gcgaggaggc cctggcccag ctcggcaccg gggaggccgg tttcgccgcc
     1561 gccgacgtgc tcgatgcctt caccaaattc gaacgcggca tcgttcgcgg cagaatcatc
     1621 gcgggcgaca aacgcatcga cgggcgcggc ccgaaagacg tccgggcgat taccgtacgg
     1681 gtgggcgtgc tgccgcgcac gcacggctcg gcgctgttta cccgcggcga gacgcaggcg
     1741 ctcgtcgtca cgacgctcgg aaccgagcgc gatgcgcagc gcatcgatgc gctcgcgggc
     1801 gagcacaaag agccgttcat gctccactac aacttccctc cgtattcggt cggcgagacc
     1861 ggattcatgg gctcgccaaa gcgacgcgaa atcggccacg gccgcctcgc caagcgcggc
     1921 atccaggccg tcatgcccga catgacggcg tacccttacg tcatccgggt ggtttcggag
     1981 gtcaccgaat cgaatggttc gagttcgatg gcgacgacct gcggcaccag tctctcgttg
     2041 atggatgccg gcgtcccgat cgatgcgccg gtcgccggcg tcgcgatggg gctcatcaag
     2101 gaggccgacg gctatgcggt cctcaccgac atcctcggcg acgaagacca tctcggcgac
     2161 atggacttca aggtcgcggg cacgaaggat ggtgtaacgg cgctgcagat ggacatcaag
     2221 atcgacggca tcacccgcga gatcatggaa acggcgatgt cgcaggcgcg cgagggccgc
     2281 ct
//
LOCUS       DASUJG010000158         5540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_535651, whole genome shotgun sequence.
ACCESSION   DASUJG010000158 DASUJG010000000
VERSION     DASUJG010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5540
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535651"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="VFX38_03295"
     CDS             complement(<1..937)
                     /locus_tag="VFX38_03295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5313919.1"
                     /translation="MTILTAIALGLWAAVLVVPWQPWRCRERLEAESERRRPESDFTV
                     LIPARNEAAVIGETLAVLAQAAPGAPIIVIDDQSDDGTAEIVRASGLEHLGLIAGTAP
                     PPGWAGKLWALEQGLASVATRRVLLLDADIRLAPGMIAALERKADEGCALVSVLAEPC
                     WRGIAARLLLPAFVYFFKLLYPFALANRARGPIAAAAGGVVLLERDVLASIGGFAAWR
                     DAIIDDCTLAAHVKRSGHRTFVGLSRGAQSLREQDLASIAAMVTRSAWVQLHESPLWL
                     LAATALLVLAFWVPPAALAFPGVPRWLGIAACLALA"
     gene            complement(934..1875)
                     /locus_tag="VFX38_03300"
     CDS             complement(934..1875)
                     /locus_tag="VFX38_03300"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX5313920.1"
                     /translation="MTFPVLSDADVARTLAMSDAIRKIEDAFRERAEGTFVAPPRFHV
                     AVERGALVFTAGAATKREKVIGFRVYGAFPDGSPDAAQLVGVFDSDTGDLKGIIIGHR
                     IGAMRTGAIGGVAIKHMARPDSRILGVLGSGLQARSQLEAAVSVCSFETIKVYSPTIE
                     HREAFAAEMAARLHRNVTAVASASEAVRESDVLICATISRSPVFPAEWLKAGAHVNTI
                     GPKFENAHELDVAVAARSSVIASDSLAQIDAYTQRFFLHGTPQRDRIRDLSEIVAGTR
                     EGRTSPADITLFCSVGLAGTEVVLANEVIRRAGIVLQ"
     gene            complement(2027..3139)
                     /gene="hpnH"
                     /locus_tag="VFX38_03305"
     CDS             complement(2027..3139)
                     /gene="hpnH"
                     /locus_tag="VFX38_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019866209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HEX5313921.1"
                     /translation="MSVPVRQQAKIAGYILAKKLKGEKKYPLVLMLEPLFQCNLACPG
                     CGKIDYESDILKQRLSVEDCLAAVDECGAPVVSIPGGEPLIHKDIKEIVDGIIARKKF
                     VYLCTNAILLEKKLDLFTPSLYLTFSVHLDGLRERHDHSVDREGVFETATRAIRAAKA
                     RGFRVNVNTTLFAGENPEQVARFFDFATNDLRVDGITVSPGYSYEHAPRQDVFMGRRQ
                     SKELFREIFKRKNGTAWPLTHSSLFLDFVCGNQDYQCTPWSNPTRNVFGWQKPCYLLV
                     DEGYAKSFRELMETTQWENYGTGRNPKCDNCMAHCGYEGTAAEDSMRHPLKAFLASRR
                     GPRLEGPFAPDRPILYERKASGLAGVAVEVDRSHPH"
     gene            complement(3143..4096)
                     /gene="ispH"
                     /locus_tag="VFX38_03310"
                     /gene_synonym="lytB"
     CDS             complement(3143..4096)
                     /gene="ispH"
                     /locus_tag="VFX38_03310"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX5313922.1"
                     /translation="MEVVLAKPRGFCAGVVRAIDVVEEALKVFGPPVYVLHEIVHNQH
                     VVNDLAGRGAVFTEDLNAVPSGAVTIFSAHGVSDQRVREARERGLRIIDATCPLVAKV
                     HMQAQKYHRGGYELIIVGHRGHPEVEGTSGRVAGPVHVVSEPEEVESLRVADPEKVAY
                     VTQTTLSVDDTRAVIAALKKRFPKIVGPEIKDICYATQNRQNAVHELAKNVDVIIVVG
                     ARNSSNSNRLREVGEHAGVRSYLVQDASEVEPDWFAANDRVGVTAGASTPEALVTEVV
                     EKLAGLGHQHVHEMAGPDENVTFRPPSELRVHLVAEAKQGG"
     gene            4220..5260
                     /gene="hpnA"
                     /locus_tag="VFX38_03315"
     CDS             4220..5260
                     /gene="hpnA"
                     /locus_tag="VFX38_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HEX5313923.1"
                     /translation="MAERVAITGATGFVGAAVVRAFLAAGYRVRAIARAGSCRDNLAG
                     LDLETVIGNLAEPDTLASALDGCDGLVHAAADYRFFVRDPAALYRVNVEGTRALMEAA
                     LAAGLRRIVHTSSVAVLGHRGDGAAADEEDAAVLGDMIGHYKRSKFLAEEAVLRLVAD
                     AALPAVIVNPTAPVGPRDRKPTPTGRMVRDAARGRMPAYLDTALNIVHVDDVAEGHRL
                     AYERGTLGRRYILGGENLALRAIFAMIARLAGRRAPRLRLAPAPLVPIAWLAETWARL
                     AGGTPFFTREELRMARQSMVFSSARAERELSYTHRPAIDAFADALAWFAEQGQLRSAD
                     GHHGSRREEALR"
     gene            5257..>5540
                     /locus_tag="VFX38_03320"
     CDS             5257..>5540
                     /locus_tag="VFX38_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014003331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HEX5313924.1"
                     /translation="MKAQADADFELQRELLAGVSRTFALTIPALPERLARVVGNAYLL
                     CRITDTIEDAASLAPAEQLDFCERFIAVVSGRGDAAAFARDFAPHLAPGA"
BASE COUNT          891 a         1806 c         1847 g          996 t
ORIGIN      
        1 cggcgagcgc aaggcaggcc gcgatgccga gccagcgcgg gacgccgggg aaggcgaggg
       61 cggcgggcgg cacccagaag gcaagcacca aaagcgccgt cgccgcgagc agccacagcg
      121 gcgattcatg cagttgtacc caggcgctgc gcgtgaccat cgccgcgatc gaagcgaggt
      181 cttgttcccg cagactctgc gcgccgcgcg agagaccgac aaacgtgcga tgtccgcttc
      241 gtttgacatg cgccgcgagc gtgcaatcgt cgatgatggc atcgcgccag gccgcaaagc
      301 cgccgatgct cgcgagaacg tcgcgttcca ggagaaccac gccgccggca gccgccgcga
      361 tcgggccgcg cgcgcgattg gcgagtgcga acggataaag cagcttgaag aaatacacga
      421 aggcgggaag cagcaatcgt gcggcgatgc cgcgccagca gggttcggcg agcaccgaca
      481 cgagcgcgca accctcgtcg gccttgcgtt cgagagcggc gatcatcccc ggcgcgaggc
      541 ggatgtccgc atcgagaagg agcacgcgtc gcgtggcgac gctggcgaga ccctgttcga
      601 gcgcccagag ttttcccgcc cagccgggcg gcggcgccgt tcccgcaatc aggccgagat
      661 gttcgagtcc gctcgcgcgg acgatctccg ccgtgccgtc gtcggattga tcgtcgatca
      721 cgattatggg ggcacccgga gccgcttgcg cgagcacggc gagcgtttcg ccgatgaccg
      781 ccgcttcgtt gcgagccgga atcaagacgg tgaaatcgga ttcgggccgg cgccgttccg
      841 attccgcttc cagacgttcc cggcaacgcc acggctgcca cggaacgacg agcactgccg
      901 cccacaggcc caacgcgatc gccgtgagga tcgtcattgc agaactatgc cggcgcgacg
      961 gatgacctcg ttcgccagca ccacttcggt acccgccaga cccaccgagc agaacaaggt
     1021 gatgtccgcg ggcgacgttc ttccttcccg tgttccggcg acgatctcgc tcaaatcacg
     1081 gattcggtcg cgttgcggcg ttccgtgcag gaagaatcgc tgggtatacg cgtcgatctg
     1141 ggcgagcgaa tcgctggcga tcacgctgct tcttgcggca accgcgacgt cgagttcgtg
     1201 ggcgttctcg aatttcggtc cgatcgtgtt gacgtgggct ccggccttca gccattccgc
     1261 gggaaaaacc ggggagcggc tgatcgtcgc gcagatcagc acgtccgatt cgcgcacggc
     1321 ttcgctcgcc gaagcgacgg ctgtgacgtt ccggtgcagc ctggccgcca tttcggccgc
     1381 gaacgcttcg cgatgttcga tggtggggct gtagactttg atggtttcga aactgcacac
     1441 cgaaacggcc gcttcgagtt ggcttcgcgc ctgcaggccg gagccgagca cgcccaagat
     1501 ccgcgaatcc ggacgcgcca tgtgtttgat cgcaaccccg ccgatcgctc cggttctcat
     1561 ggcgccgatc cgatgtccga tgatgatgcc cttgagatcc ccggtatcgc tgtcgaaaac
     1621 gccgaccagt tgcgcggcat cgggcgatcc atcggggaag gccccgtaca cgcgaaagcc
     1681 gatcaccttt tcccgcttcg tggcggcgcc ggcggtaaac accaaggctc cccgttcgac
     1741 cgccacgtgg aatcgcggcg gcgcaacgaa cgttccttcc gcccgttcgc gaaacgcatc
     1801 ttcgattttg cgaatcgcgt cgctcatggc gagggtacga gcgacgtccg cgtcactcag
     1861 gacaggaaag gtcatcgctg ctgattcccg gcgccgcaag ccttgcgaat tatagtgccg
     1921 cgggctgcgg cctccgttcc gactcgcccg aagcgctggt gatcgtgagc cagaagccgg
     1981 aacatcgccg cccggcgcgg ctacgcgtac ctccggggct cttccgtcag tgcgggtggc
     2041 tgcgatccac ctcgacggcg acgccggcga ggccgctcgc cttgcgctcg tagaggatcg
     2101 ggcgatcggg cgcgaacgga ccttcgaggc gcggaccgcg gcggctggcg agaaaggctt
     2161 tcagcggatg gcgcatgctg tcttcggccg cggtgccctc gtagccgcaa tgcgccatgc
     2221 agttgtcgca cttcggattg cgcccggtgc cgtagttttc ccactgcgtg gtttccatca
     2281 gttcgcggaa gctcttcgca tagccctcgt ccacgagcag atagcagggc ttctgccagc
     2341 cgaagacgtt gcgcgtcggg ttgctccagg gcgtgcactg atagtcctgg ttgccgcaga
     2401 cgaagtcgag aaagagactc gagtgcgtga gcggccaggc ggtgccgttc ttgcgcttga
     2461 agatttcgcg gaagagttcc ttcgattgcc gccggcccat gaacacatcc tgacgcggcg
     2521 cgtgttcgta gctgtagccg ggcgagacgg tgatgccgtc cactcgaaga tcgttggtcg
     2581 cgaagtcgaa gaagcgcgcg acctgttcgg gattctcgcc ggcgaagagc gtggtgttga
     2641 cgttgacgcg aaagccccgc gccttggccg cgcggatggc gcgggtcgcc gtttcgaaga
     2701 caccctcgcg atccacgctg tggtcgtggc gctcgcgcag accgtcgaga tgcaccgaga
     2761 aggtgagata gagcgagggc gtgaacagat cgagtttctt ctccagcaga atcgcgttgg
     2821 tgcacaggta cacgaacttt ttgcgcgcga tgatgccgtc cacgatctcc ttgatgtcct
     2881 tgtggatcag cggctcgccg ccggggatgg aaacgaccgg cgcgccgcat tcgtccaccg
     2941 ccgcgaggca gtcctcgacc gagaggcgct gcttgaggat gtcgctttcg taatcgatct
     3001 tgccgcagcc gggacaggcg aggttgcact gaaacagcgg ctccagcatc agcaccagcg
     3061 gatacttctt ctcgcccttg agcttcttgg cgagaatgta gccggcgatc ttggcctgtt
     3121 gacgcacggg tacggacatg attcagcctc cttgtttcgc ttccgccacg agatgcacgc
     3181 gcaattcgga cggcgggcgg aaggtgacgt tttcgtcagg gccggccatc tcgtggacgt
     3241 gctgatgtcc gagcccggcg agcttctcca ccacttcggt gacgagtgcc tccggcgtcg
     3301 aggcgccggc ggtcacgccg acgcgatcat ttgccgcgaa ccagtcgggt tcgacttcgc
     3361 tcgcgtcctg caccaggtag gagcgcactc cggcgtgttc gccgacctcg cgcaggcggt
     3421 tcgagttgga actgttgcgt gcgccgacga cgatgatgac gtcgacgttc ttcgcgagct
     3481 cgtgcaccgc gttctggcgg ttctgggtcg cgtagcagat gtccttgatt tccgggccga
     3541 cgattttcgg aaagcgtttc ttcagcgccg cgatcacggc gcgggtgtcg tccaccgaga
     3601 gggtcgtctg ggtcacgtag gcgacctttt cgggatcggc tacccgcagg ctttccactt
     3661 cctccggctc cgagaccaca tgcaccggac ccgcgacccg cccgctcgtg ccttccacct
     3721 cgggatggcc gcgatgcccg acgatgatga gttcatagcc gccgcgatga tatttctgcg
     3781 cctgcatgtg caccttcgcg acgagcgggc aggtggcgtc gatgatgcgc agcccgcgct
     3841 cgcgcgcctc gcgaacgcgc tgatccgaca ccccgtgggc gctgaaaatc gtgacggcgc
     3901 cggagggtac ggcgttgagg tcttcggtga agacggcgcc gcgtccggcg agatcgttca
     3961 cgacatgctg attgtgaacg atctcgtgca aaacgtagac cggcggtccg aaaaccttca
     4021 gcgcctcctc gaccacgtcg atggcgcgca cgacgccggc gcagaatccc cgtggtttgg
     4081 caagcacgac ttccatttcg ctcgaatcct cctcctcgtg agggcggtgc aggttgcgcc
     4141 ctaagtgctg aaattataac gccttggctg cgtcccgcgg tacactgtcg tttggcttcg
     4201 tgctcgccgc gaggagaaaa tggccgaacg cgtggcgata acgggggcta cgggtttcgt
     4261 cggcgccgcc gtggtacgcg cgtttctcgc cgccggctat cgggtgcgcg cgatcgcgcg
     4321 cgcgggctcg tgccgggaca acctcgcggg cctcgatctg gaaaccgtga tcggcaacct
     4381 cgccgagccg gatactttgg cgagcgctct cgacggttgc gacgggctcg tgcacgcggc
     4441 cgccgactat cgtttcttcg tgagagaccc ggccgcgctt tatcgcgtga acgtggaggg
     4501 cacgcgcgcg ctcatggaag cggcgctcgc cgcgggcttg cgccgcatcg ttcatacctc
     4561 gagcgtcgcc gtcctcggac atcgtggcga cggcgctgcc gccgacgagg aagacgcagc
     4621 ggtgctcggc gacatgatcg ggcattacaa gcgctcgaag tttctcgccg aggaagcggt
     4681 cctgcgcctc gtcgcggatg cggcgctccc cgccgtcatc gtcaacccca ccgcgccggt
     4741 cggcccgcgt gatcgcaaac cgactccgac gggaaggatg gtgcgcgatg ccgcgcgcgg
     4801 gcggatgccg gcctatctcg acaccgcgct caacatcgtg cacgtggatg atgtcgcgga
     4861 ggggcaccgg ctcgcctacg agcgcgggac gctcgggcga cgctacattc tcggcggcga
     4921 gaatctcgcg ctgcgagcga ttttcgccat gatcgcgcgt cttgccgggc ggcgtgcgcc
     4981 gcgcctgcgc ctcgcgccgg caccgctggt cccgatcgcg tggctggcgg aaacctgggc
     5041 gcgactcgca ggcgggactc cgttttttac gcgcgaggaa ttgcggatgg cgcgccaatc
     5101 gatggtcttc agttcggcgc gcgcggagcg cgaactcagc tacacgcacc gcccggcgat
     5161 cgatgccttc gccgatgcgc ttgcctggtt cgcggagcag ggacaattgc gaagcgccga
     5221 cggccaccac ggttcgcgcc gcgaagaggc tttgcgatga aggcgcaggc cgacgccgac
     5281 ttcgaattgc agcgcgaact tctcgcgggc gtttcgcgca cctttgcgct cacgattccg
     5341 gcgctgcccg agcgcctggc gcgcgtcgtc ggcaacgcct atcttctctg ccgcatcacc
     5401 gataccatcg aggatgcggc gagcctcgct ccggcggagc aactggattt ttgcgagcgc
     5461 ttcatcgcgg tcgtctccgg gcgcggcgac gcggctgcct tcgcgcgcga ctttgctccg
     5521 catctcgccc ccggcgcgag
//
LOCUS       DASUJG010000159         2008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_903270, whole genome shotgun sequence.
ACCESSION   DASUJG010000159 DASUJG010000000
VERSION     DASUJG010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903270"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /gene="proS"
                     /locus_tag="VFX38_03325"
     CDS             complement(<1..846)
                     /gene="proS"
                     /locus_tag="VFX38_03325"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018872146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX5313925.1"
                     /translation="MRLSQVLLQTLRTAPAEAETVSHRLMLRAGLIRQHAAGIYSWLP
                     IGLRVLRKIETIVREEMQRIGAVELLMPAVQPAELWQESGRWAKYGPELLRFTDRRER
                     EFCFGPTHEEVITDLVRNIVSSYRQLPLTLFQIQTKFRDELRPRFGVMRAREFVMKDA
                     YSFHLDEDSLAETYESMRDAYSRILERIGVEYRVVEADTGAIGGEASHEFHVLADSGE
                     DVIAYAPKGSYAANLELARARKPRGPRPAPTAKMARVATPGVRSIGELASFLDIAPER
                     CAKTLL"
     gene            909..1925
                     /gene="ilvC"
                     /locus_tag="VFX38_03330"
     CDS             909..1925
                     /gene="ilvC"
                     /locus_tag="VFX38_03330"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF004017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX5313926.1"
                     /translation="MAVVSQAEIEAASLEDAKVAVLGYGSQGRAQALNLRDSGIFVTV
                     GLRSGSARRAAAQADGLPVLPPAEAAADSSVVAMLVPDEVQPRFYREKIEPALTPGST
                     LVFAHGFNIHYGRIEPRADLDVVLVAPSAIGEELRAQYLAGRGTAGLVAVAQDASGRA
                     RSRALAYARALGHDRAAIIDTSFAEETETDLFAEQTLLVGGLTGLIEDAFETLVAAGY
                     QEEIAYFCCFEEVKFMADMLYAQGLAGLWRRISSTAAFGAVESGPRVVNPQSRAAMEA
                     VLEDIRAGRFAARLASEMQAGAPALAARAARLRDHRLEALHRRFGPRPAAPGGGPRAR
                     DVAS"
BASE COUNT          303 a          640 c          722 g          343 t
ORIGIN      
        1 caggagcgtt ttcgcgcagc gctccggcgc aatgtcgaga aaactcgcga gttcgccgat
       61 gctgcgaacg cccggcgtcg cgacgcgggc cattttcgcg gtgggggcgg ggcgcggccc
      121 gcgcggcttg cgtgcccgcg cgagttcgag gtttgcggcg tagctgcctt tgggcgcata
      181 cgcgatcacg tcttcgcccg aatcggcaag cacgtgaaat tcatgcgatg cctcgccgcc
      241 gatcgctccg gtgtctgcct cgacgacgcg gtactcgacc ccgatccgct cgaggatgcg
      301 gctgtaggca tcgcgcatgc tctcgtaggt ttcggcgagc gaatcctcgt cgagatggaa
      361 ggagtaggca tccttcatca cgaattcgcg ggcgcgcatg acgccgaagc gcgggcgcag
      421 ttcgtcgcgg aacttggttt gaatctggaa gagtgtgagc ggcagttgcc ggtaggagct
      481 gacgatgttg cgcaccagat cggtgatgac ttcctcgtgc gtcgggccga agcagaattc
      541 gcgttcgcgc cggtccgtga agcgcaacag ctccgggccg tatttcgccc agcgccccga
      601 ctcctgccac agttccgccg gctggacggc gggcatcagc agttccacgg cgccgatgcg
      661 ctgcatttcc tcgcgcacga tggtctcgat cttgcgcagc acccgcagcc cgatcggcag
      721 ccaactgtag atgccggcgg catgctggcg gatgagcccg gcgcgcagca tcaatcggtg
      781 actcaccgtt tcggcttcgg ccggggccgt gcgcagcgtc tgaaggagaa cctgggatag
      841 gcgcaccgga gcgtcccgtg gggcgaaaga ggcttattct atcggcccca atagcgcgga
      901 gtccgtgcgt ggcggtggtt tcgcaggcgg aaatcgaagc ggcgagtctc gaagacgcga
      961 aagttgccgt gctcggctac ggatcgcagg gccgtgcgca ggccctgaat ctgcgcgatt
     1021 cggggatctt cgtgacggtg ggactgcgct ccggctctgc gcgaagagcg gcggcgcagg
     1081 cggacggcct tcccgttctg ccgcccgccg aggcggcggc ggattcttcc gtggtcgcga
     1141 tgctggtgcc cgacgaggtc caaccgcgct tttaccgcga aaagatcgag ccggcactga
     1201 cgccgggcag taccctcgtt ttcgcgcacg gtttcaatat tcactacggg cgcatcgagc
     1261 cacgcgccga cctcgacgtg gtgctggtcg ccccttcggc gatcggcgag gagctgcgcg
     1321 cgcaatatct cgccggtcgc ggcacggccg gcctcgtcgc ggttgcgcag gacgcgagcg
     1381 ggcgggcgcg aagccgtgcg cttgcctacg cgcgcgcgct cggccacgat cgggcggcca
     1441 tcatcgacac gagtttcgcc gaagaaaccg aaaccgatct gttcgccgaa cagaccctgc
     1501 tcgtcggcgg actgaccggc ttgatcgagg atgccttcga gactttggtg gcggccggtt
     1561 atcaggaaga gattgcctac ttctgctgtt tcgaagaggt gaaattcatg gcggacatgc
     1621 tctacgcgca gggcctcgcg gggctctggc ggcgcatttc ctccaccgcc gccttcggtg
     1681 ccgtcgagag cggcccccgg gtggtgaacc cgcagtcgcg cgcggcgatg gaggcggtgc
     1741 tcgaggatat ccgcgccggg cgctttgccg cgcggctcgc gagcgagatg caggcgggcg
     1801 cgccggcgct cgcggcgagg gccgcgcgtc tgcgggatca tcgcctggag gcgctgcatc
     1861 ggcgcttcgg cccgcgcccg gccgcgcccg gcggtggccc gcgagcgcga gacgtggcat
     1921 cctaatggcg atggagccgc gactgaaatg ggtgcgcacg caggagcgcg acgctccggg
     1981 gggcgatggg gaggatccga atccgcac
//
LOCUS       DASUJG010000160         3178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_292255, whole genome shotgun sequence.
ACCESSION   DASUJG010000160 DASUJG010000000
VERSION     DASUJG010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292255"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..948)
                     /locus_tag="VFX38_03335"
     CDS             complement(34..948)
                     /locus_tag="VFX38_03335"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX5313927.1"
                     /translation="MNPRCFFVIGAQKSGTTALFEYLRHHPEVYMPPEKEAEFFTGSD
                     TTPAAWAEYRRRLLTSPRSLRVVGTASPQYMGDPAVPARMAALVPDALLIAVLRDPVE
                     RAVSHYKMAVRRGDERRDFARAVREQLQPAALEKTRALPFREVAERDAYVVWGEYARI
                     LRGFLHRFSREQLLLVYSEDLRERPREALARVCRHLGIAEGYSPPNLGKLYHVGGARR
                     KLPGLEKVLRSAVARGLIRLAVRKRRYKRMQMQVERWNAKPTTLVVPGETYAALADHY
                     AGDVAEVSRLCGIAPRWFLHRGEATVTL"
     gene            complement(955..2661)
                     /locus_tag="VFX38_03340"
     CDS             complement(955..2661)
                     /locus_tag="VFX38_03340"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5313928.1"
                     /translation="MKNCWTFVLGIMWVVLTALVMSLLPTGCAAAAEPPAVASSLLPA
                     RVWKAAQDRVVAGEYPAVVIGVVNGGKSRVYGFGKLANGKTPDAATVFEIGSITKTFT
                     ALLLADAVKKRSVALGEPVAKLLPGFVIPSRDGKQITLENLADQHSGLPRLPTNFDPA
                     NPADPYAGYDADRLKAFLGSYDLPRDPGSAYEYSNLGFGLLGYALARHADMTYDALVD
                     ERILQPLGMKMSGVAMNDATRAQLAAGHDATEKSAANWDFGVLAGAGALKSDGADMLR
                     YLEANMGVLKTPLYAAMRLAQEPLLPANGKERVGLAWMTLPTKGGDVIWHNGMTGGYA
                     SFIGFTADGKRGVVILTNVQRPVDDLGFATLLADWSLAPAEKVISLPASALDDYAGSY
                     RLATKFVLTISLQGDQLYARATGQGAFPIFPSAKDEFFARMSGIRISFERNAQGKIAG
                     LVLHQNGDRRAPRISAAQAAAAATSTPIVTLPAATLREYAGQYRLAPGAVFDVTLAGD
                     QLSVQLSGQPAFPVYASAKDKFFYTVVDARIEFVRDGEGNVVALVLHQNGQARRAPRV
                     QR"
     gene            complement(2726..3067)
                     /locus_tag="VFX38_03345"
     CDS             complement(2726..3067)
                     /locus_tag="VFX38_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX5313929.1"
                     /translation="MSGESVPETLLGLDRLVHEPARLLILSVLAGAKEVEFGFLEKMS
                     GLSKGNLSSHMSKLEAAGLIGVRKRFRAKRPLTELHLTVKGRRSLEAYRRQLATVMQS
                     LTAEPADGKRR"
BASE COUNT          550 a         1070 c         1000 g          558 t
ORIGIN      
        1 ctcgccgact acgcgaccga aattctctga ccttcagagg gtaacggttg cctcgccgcg
       61 atgcaggaac cagcgcggag cgatcccgca aaggcggctg acttcggcga catcccccgc
      121 gtaatggtcc gccagtgccg catacgtttc gcccggcacg acgagcgtcg tcggcttcgc
      181 gttccagcgc tcgacttgca tctgcatccg tttatagcgg cgtttgcgca cggcgagacg
      241 gatgagaccg cgggcaaccg ccgaacgcag gactttttcg agccccggga gtttccgccg
      301 cgcgccgcca acgtgataaa gcttcccgag gttcggcggc gaatagcctt cggcgatgcc
      361 gagatggcgg caaacccgcg cgagcgcttc tcgcggacgt tcgcgcagat cctccgaata
      421 cacgagcagc agttgctcgc gcgaaaaccg atggagaaag ccgcgaagaa tccgtgcgta
      481 ttccccccag acgacgtagg catcgcgctc cgccacttcc cgaaacggca gcgcgcgggt
      541 tttctcgagc gccgccggct gcagttgttc gcgaacggcc cgcgcgaagt cgcgccgctc
      601 gtcgccgcga cgaaccgcca tcttgtaatg cgacacggcc cgctcgacgg gatcgcgaag
      661 gaccgcgatc agaagggcgt cgggaacgag ggccgccatg cgcgccggca ccgccggatc
      721 gcccatgtac tgcgggctcg cggtaccgac gacccgcaag gagcgcggcg aagtcagaag
      781 ccttctgcgg tactcggccc aggccgccgg cgtcgtatcg cttccagtga aaaactccgc
      841 ctccttctcc ggcggcatgt acacctccgg gtgatgacga agatattcga aaagggcggt
      901 cgttccgctc ttctgtgcgc cgatgacgaa aaaacagcgc gggttcatgc ccggctatcg
      961 ctgcacgcgc ggcgcgcgcc gggcttggcc gttctgatgc agcacgaggg cgaccacatt
     1021 cccctcgccg tcgcggacaa actcgattcg cgcatccacc accgtgtaga agaatttgtc
     1081 tttggcgctg gcatagaccg ggaacgcggg ctggccgctc aactggacgc tcagttgatc
     1141 gcccgcgagc gtcacgtcga acacggcacc gggcgcgagt cgatactgcc cggcgtattc
     1201 gcgcagcgtc gccgcgggaa gcgtgacgat cggcgtcgag gtcgcggccg ccgccgcctg
     1261 cgcggcgctg attcgcgggg cccgacggtc gccgttctga tgcaggacga ggccggcgat
     1321 cttcccctgc gcgtttcgct cgaagctgat tcggatgccc gacatcctcg cgaaaaattc
     1381 gtccttcgcg ctcggaaaaa tcgggaaggc accctgcccc gtcgcccgcg cgtagagttg
     1441 atcgccctga agcgaaatcg tgagcacgaa tttcgtcgcg agccgataac tgccggcgta
     1501 gtcgtcgagt gcgctcgccg gcagggagat caccttttcc gccggcgcaa gcgaccagtc
     1561 cgcgagcagg gtcgcgaagc cgaggtcgtc caccggccgc tgcacattgg tgaggatcac
     1621 gacgccgcgc tttccgtcgg cggtgaagcc gatgaaactc gcgtacccgc cggtcattcc
     1681 gttgtgccag atcacgtcgc cgcccttggt cgggagggtc atccacgcga gaccgacccg
     1741 ttccttgccg ttcgccggca gcaggggttc ctgcgcaagg cgcatcgccg cgtacagggg
     1801 agttttcaga acacccatgt tggcttcgag gtagcgcagc atgtcggcgc cgtcgctctt
     1861 gagcgcgccc gcgcccgcga gcacgccgaa atcccagttc gccgccgact tctcggtcgc
     1921 atcatggccc gcggcaagct gcgcgcgcgt ggcatcgttc atcgcgactc cgctcatctt
     1981 catgcccagc ggttgcagaa tccgctcgtc gaccagcgca tcgtacgtca tgtctgcgtg
     2041 gcgcgcgagg gcatagccga ggaggccgaa gccgagattc gagtattcgt aggcgctgcc
     2101 cggatcgcgc ggcaggtcgt aactcccgag aaatgccttg agcctgtctg catcgtagcc
     2161 ggcataggga tcggccgggt tcgcggggtc gaaattggtc ggcagacgcg gcaggcccga
     2221 gtgctgatcg gcgagattct cgagcgtaat ctgcttgccg tcgcgcgaag gaatgacgaa
     2281 tcccggcagg agcttcgcga ccggttcgcc gagcgcgacg ctcctctttt tcaccgcgtc
     2341 ggccagcagc agggccgtga aagtcttggt gatcgagccg atctcgaaca cggtcgcggc
     2401 atccggcgtt ttgccattcg caagtttgcc gaaaccgtac acccgacttt tcccgccatt
     2461 caccacgccg atcacgaccg ccgggtactc gcccgcgaca acccggtcct gcgccgcttt
     2521 ccacactcgc gccgggagca gcgaggaggc cacggccggc ggttccgcgg cggcggcaca
     2581 gcccgtcggc aagagcgaca tcacgagggc cgtcaacacg acccacatga ttccgagcac
     2641 gaaggtccaa cagtttttca tcgcatcgtc tcctctctcg tcggttgttc ggttgttcgg
     2701 ttgttcgatt cgaaaagttg ctgtctcatc tccgcttgcc atcggcaggt tcggcggtca
     2761 gggactgcat cacggttgcc aattgccggc ggtacgcttc cagggacctg cgacccttca
     2821 cggtaaggtg caattcggtg agcggccgct tggcgcgaaa ccgcttccgg actccgatca
     2881 ggcccgccgc ctcgagcttg ctcatgtgac tggaaagatt gcccttgctg agaccgctca
     2941 ttttttcgag aaagccgaat tcgacctcct tggcgcccgc cagcacgctc aaaatcagca
     3001 gccgcgccgg ctcgtgcacc aggcggtcga gcccaagcaa cgtttccgga accgattcgc
     3061 cgctcactat cggttcaccg tcgttccctg cagtgccgca aggcgcgtcc gcaggcgcaa
     3121 gaagcggcga tggtcgccga ttccggcggc gatcatcgcc agcgccgcga gcggaaac
//
LOCUS       DASUJG010000161         3215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1269451, whole genome shotgun sequence.
ACCESSION   DASUJG010000161 DASUJG010000000
VERSION     DASUJG010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269451"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /gene="rnpB"
                     /locus_tag="VFX38_03350"
     ncRNA           complement(<1..291)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFX38_03350"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(298..1218)
                     /gene="rfbA"
                     /locus_tag="VFX38_03355"
     CDS             complement(298..1218)
                     /gene="rfbA"
                     /locus_tag="VFX38_03355"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005319086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEX5313930.1"
                     /translation="MTRKGIILAGGTGSRLYPITRSMSKQLMPVYDKPLVYYPLTTLM
                     RAGIREILLISTPEDLPAFRRLLGDGSQWGMTFEYAEQPRPEGLAQAFVIGEAFLGGS
                     PAALVLGDNLFYGEGLQEQLDRAVARKRGATVFAYRVGDPERYGVVSFNEAGRAIAIE
                     EKPKAPRSHYAVTGLYFYDNAVVDIAKGVKPSARGELEITDVNRVYLERGELTVNCLG
                     RGVAWLDTGTHNTLLQAANFIEAIQNRQGLLIACPEEVAYRHGFIGRDALLDLARPLA
                     KTAYGKYLRQITELEEEQAAATSTAMRRVK"
     gene            complement(1271..2467)
                     /locus_tag="VFX38_03360"
     CDS             complement(1271..2467)
                     /locus_tag="VFX38_03360"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX5313931.1"
                     /translation="MTAIRAAYIVSPLRTPVGKAPRGMFRRVRPDDLLVHCIRGVLAA
                     NPGLDPARIEDLVVGCAMPEGEQGLNVARMAALVAGLPDSVPGMTVNRFCSSGLQAIA
                     IAADRVRTGDADLVLAGGVESMSLVPMGGHHPAYNAAIFARREDYGIAYGMGITGEKV
                     AEQFQVSREEQDAWAAESHARALAAIASGEFAAEILPYEITRARPGGADGGCAIERRT
                     VDTDEGPRPGSTPEVLAKLRPVFAAKGSVTAGNSSQMSDGAALCIVASEAAVKEHGLA
                     PVARLLSFRVAGVPPEIMGIGPKEAVPKALAAAGLALKDMDWIELNEAFAAQTIAVVR
                     ALGLDPATTNPLGGAIALGHPLGCTGAKLTATALHGLARRQGRYALVTMCIGTGMGAA
                     GIFERL"
     gene            complement(2476..>3215)
                     /locus_tag="VFX38_03365"
     CDS             complement(2476..>3215)
                     /locus_tag="VFX38_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5313932.1"
                     /translation="AAGTLDPAKMIAEFQRTSLALRYSAVPTVAAVRGMALGGGCEFL
                     LHCDRVVAALESHIGLVELGVGLVPAGGGTKEFALRAAAETGPGWLFDRIAQRFELVG
                     TAKVAGSAEEARELGFLREADVVVFHPDELLHVARAQALALHESGYRPPRAPAGIPVM
                     GATGIANIKARLVNLLEGRFISEYDFELAARVADILCGGDVEEGERVNEEYLLALERK
                     HFLELMAQEKTRARIEHTLKTGKPLRN"
BASE COUNT          520 a         1117 c         1033 g          545 t
ORIGIN      
        1 gaagcgcgac gcggatcacg ccggcggcac cgctgccgct cgcttcccta ttcggccttg
       61 ctccgggcgg ggttttccat gccgtcaccg ttaccggcaa cgcggtgcgc tcttgccgca
      121 ccgtttcacc cttgcctgtg ccgacagcaa agctgccggc catcggcggt ctggtttctg
      181 tggcactttc cgtgggctcg cgccccccag gcgttacctg gcgccctgat ccgaggagcc
      241 cggactttcc tctcctcccc gtcggggcga agcgatcgtc tggccgactc ccgcccccta
      301 ttttacccgc ctcatggctg tcgaggtcgc ggccgcctgc tcctcttcaa gctcggtgat
      361 ctggcgcaga tacttgccgt aggcggtctt ggcgaggggg cgcgcaaggt cgagcagggc
      421 gtctcgcccg atgaatccgt ggcggtaggc gacctcttcc ggacaggcga tgagaaggcc
      481 ctggcgattc tggatcgcct cgatgaaatt tgcggcctgc agcaaggtgt tgtgcgtccc
      541 cgtgtcgagc caggcgaccc cccgcccgag acagttgacg gtgagttccc cgcgctcaag
      601 atagacgcga ttgacgtcgg tgatctcgag ttcgccgcgc gccgagggct tcacaccctt
      661 ggcaatgtcc accaccgcat tgtcataaaa gtacaggccc gtcaccgcat aatgcgagcg
      721 cggagccttg ggcttttcct cgatcgcgat cgcgcggccc gcctcgttga agctcacgac
      781 gccgtaccgc tccgggtcgc ccacccggta ggcgaatacc gtggcgccgc gcttgcgggc
      841 gacggctcga tccaactgtt cctgcaggcc ctcgccgtag aaaagattgt cgcccagcac
      901 aagcgccgcg ggcgagccgc cgagaaacgc ctcgccgatg acaaacgcct gggcgagacc
      961 ttccggccgg ggctgttcgg catactcgaa cgtcatgccc cattgggagc cgtcgcccag
     1021 cagacgccgg aaggcgggca gatcctccgg ggtcgagatc agcaggatct cgcggatgcc
     1081 ggcacgcatc agcgtcgtga gcgggtaata gacaagcggc ttgtcgtaga cgggcatgag
     1141 ctgtttgctc atgctgcgcg tgatcggata gagacgcgaa ccggtcccgc cggccagaat
     1201 aattccctta cgggtcatgg cgaaccaccg acatcgagca tcccccgcga ttccggccta
     1261 tcaccataac tcaaagacgc tcgaaaatgc cggccgcacc cattcccgtt ccgatgcaca
     1321 tggtcacgag cgcgtaccgc ccctggcgcc gggcgagccc gtgaagcgcc gtcgcggtaa
     1381 gtttggcacc ggtgcaaccg agcggatggc cgagcgcgat cgccccgccc aggggattgg
     1441 tcgtcgccgg gtcgagcccg agcgcccgca cgaccgcaat cgtctgggcc gcgaaggctt
     1501 cgttgagttc gatccagtcc atgtccttga gcgcgagccc cgccgcggcg agcgccttgg
     1561 gcaccgcttc tttcggcccg attcccatga tctcgggcgg caccccggcg acccggaagc
     1621 tcaggagccg cgcgaccggc gcaagtccgt gttccttgac ggccgcctcg ctcgcgacga
     1681 tgcaaagcgc cgcgccgtcg ctcatctgcg agctgttgcc cgccgtcacg ctgcccttcg
     1741 ccgcaaacac cggacgcagt tttgccaaca cctccggagt cgaacccgga cgcgggcctt
     1801 cgtcggtatc cacggtcctg cgctcgattg cgcatccgcc gtccgcgcct ccgggccggg
     1861 cgcgggtgat ttcataggga aggatttcgg cggcgaactc gccgctcgcg atggccgcga
     1921 gtgcccgtgc atgactttcg gccgcccagg catcctgctc ttcgcggctc acctggaact
     1981 gttcggccac cttctcgccg gtgatcccca tcccgtaggc gatgccgtag tcttcgcgac
     2041 gcgcgaagat cgccgcattg taggcggggt ggtgcccgcc catcgggacg agactcatgc
     2101 tttcgacccc gcccgcaagg accaagtccg catccccggt gcgcacgcgg tcggcggcga
     2161 tcgcgatcgc ctgaagcccg gaggagcaga agcgattgac ggtcatgccg gggaccgaat
     2221 cgggaaggcc ggcgacgagc gccgccatgc gcgcgacgtt caatccctgc tcgccctcgg
     2281 gcatggcaca gccgacgacg agatcctcga tgcgcgcagg atccagcccc ggattcgcgg
     2341 cgagcacgcc gcggatgcaa tgaaccagca gatcgtcggg gcgcacgcgc cggaacatgc
     2401 cgcgcggcgc cttgccgacg ggcgttctga gcggggagac gatgtaagca gcgcgaatcg
     2461 cggtcatgtc gcttctcagt tcctgagcgg ttttccggtc ttgagcgtgt gctcgatacg
     2521 cgcgcgggtt ttctcctgcg ccatgagttc gagaaaatgt ttgcgctcga gcgcaagcaa
     2581 atactcctcg ttcacccgtt cgccttcctc gacatcgccg ccgcagagaa tgtcggccac
     2641 ccgcgctgcg agttcgaagt cgtactcgga gataaaccgg ccttcgagca gattcacgag
     2701 ccgcgccttg atgttggcga tgccggtcgc gcccatgaca ggtatgcccg cgggcgcgcg
     2761 cggcggccga tagccggact cgtgaagcgc gagcgcctgc gccctggcga catgaagcag
     2821 ttcgtccggg tggaacacta cgacgtcggc ttcgcgcaga aaacccagtt cacgcgcctc
     2881 ctcggcgctg ccggcgacct tcgccgtgcc gacgagttcg aagcgttggg caatgcgatc
     2941 gaagagccag ccgggcccgg tttccgcggc cgcacgcagc gcgaattcct tggtgccgcc
     3001 gcccgccgga acgagaccca caccaagttc cacgagtccg atatggcttt cgagggccgc
     3061 gaccacgcga tcgcaatgca gcagaaattc gcagccgccg ccgagcgcca tgccgcgcac
     3121 cgcggcgacc gtgggcaccg ccgagtagcg cagcgcgagc gaggtccgct gaaactcggc
     3181 gatcattttg gcgggatcga gcgtgccggc ggcga
//
LOCUS       DASUJG010000162         2740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1515683, whole genome shotgun sequence.
ACCESSION   DASUJG010000162 DASUJG010000000
VERSION     DASUJG010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1515683"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VFX38_03370"
     CDS             complement(<1..187)
                     /locus_tag="VFX38_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003150020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX5313933.1"
                     /translation="MNRRAGGYSLVELLVALALSLFLIAGIAVMYFSTSQSYHVEQQT
                     AALEEHQRLVATFVGSVI"
     gene            complement(184..672)
                     /gene="pilV"
                     /locus_tag="VFX38_03375"
     CDS             complement(184..672)
                     /gene="pilV"
                     /locus_tag="VFX38_03375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HEX5313934.1"
                     /translation="MNAGARGFSLLEALIALLVIAIGLLGIASMQAASVYRTHVGALN
                     GLASLEAQSMAARMTANPGAFPVGSSVFAYATTNAADVSSSTDCESAYCDSDAMALYD
                     LKQWGDGLASALPDGKGAVACAGDPPQCTVTVSWLEKTMTPAAAGTAPIATRSYRLVV
                     RP"
     gene            complement(669..1223)
                     /locus_tag="VFX38_03380"
     CDS             complement(669..1223)
                     /locus_tag="VFX38_03380"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEX5313935.1"
                     /translation="MEGKSGGFTLIEMLVTIGLLAILSAIALPSYRSFVAGNRAATQL
                     NTFVADFQYARSDAMLNGTDVSMCASADGQSCAADGDWNQGWIVFMDPNDDRVVGTGD
                     TLLRVHPALLAGDTLIGGGNLERHVTFNRFGMLASLYNGTIVRRTAPVRTANIRCIVV
                     SGVGKVRAQSGASCVPAPGPPPGT"
     gene            complement(1333..>2740)
                     /locus_tag="VFX38_03385"
     CDS             complement(1333..>2740)
                     /locus_tag="VFX38_03385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HEX5313936.1"
                     /translation="ADFASIQSLDPGSGELRLLAQRGFIPGAAEFWMRVGPDGASTCA
                     QALRSGRRVVVGDVEAEVSVAGSPDLELYRRTGIRALQSTPLVARGGRLLGMLSTHWG
                     EPHWPSEDELDLFDVLARQAADFLERLEAHETLHASEARLRAIFDGTYEYIVLLNSEG
                     RVLDANRAALDSGPWTREDLIGRPYPETPWFAGTPGAAEELRKALARAAAGEFVRYEL
                     AIARPAGEVLTFDFSLHPVSEEPGAAALIVAESRDISRRKQTEEHRQLLVNELNHRVK
                     NTLATVQSLAIQTLAGSQLATEVAKTFEGRLLALAQTHNLLTSARWGDVGLRELLARE
                     LAPFGEDGDGRLAIAGDDALLRPKAALALGMALHELATNASRHGAWSDPAGRVRIDSA
                     LLDGGRGLRLEWKESGGPEVAKPHRRGFGTRLIQAGLTHELAGEVRIDYLPGGLHCAI
                     EIPLASASGEAVEAQTRP"
BASE COUNT          430 a          921 c          977 g          412 t
ORIGIN      
        1 ggatgacgga gccgacgaag gtcgcgacga ggcgctgatg ttcctcgagc gccgccgtct
       61 gctgctccac gtgatagctc tggctggtcg agaaatacat cacggcgatg ccggcgatga
      121 gaaagagact caaggcgagc gcgaccagca gttcgaccag cgaatagccg ccggcgcgcc
      181 ggttcacgga cgcaccacga ggcggtagct gcgggtcgca atcggcgccg ttcccgcggc
      241 cgcgggcgtc atcgtcttct cgagccagct cacggtcacg gtgcactgcg gcggatcgcc
      301 ggcgcaggcg acggcaccct tgccgtcggg aagcgcgctc gcgaggccgt cgccccattg
      361 tttgagatcg tagagcgcca tcgcatcgga gtcgcaatag gcactctcgc agtccgtgga
      421 agaactcacg tccgcggcat tcgtggtggc gtaggcgaag accgaagagc cgacgggaaa
      481 ggcgccggga ttggcggtca tccgcgccgc catgctctgg gcctcgagcg aggcgagtcc
      541 gttcagcgcg ccgacgtggg tgcgatagac cgaggcggcc tgcatgctcg cgatcccgag
      601 caggccgatc gcgattacga ggagcgcgat cagtgcctcg agcagactga agccgcgcgc
      661 gcccgcgttc atgttcccgg cggcggtcct ggggccggca cgcaggaggc gccggactgg
      721 gcacggacct tgccgacgcc ggagacgacg atgcagcgga tgttcgcggt gcgcaccggc
      781 gccgtgcgcc tgacgatcgt gccgttgtag aggctcgcga gcatgccgaa gcgattgaag
      841 gtgacgtggc gctcaaggtt gccgccgccg atgagggtgt cgcccgcgag aagtgcggga
      901 tgcacgcgca gcagggtgtc gcccgtcccg acgacgcggt cgtcgttcgg gtccatgaac
      961 acgatccagc cctggttcca atccccgtcc gccgcgcagc tctgcccgtc cgcgctcgcg
     1021 cacatggaaa cgtcggtgcc gttcagcatc gcgtccgagc gcgcgtactg gaagtcggcg
     1081 acgaaggtat tcaactgggt cgccgcgcga ttgccggcga caaaactgcg gtacgacggc
     1141 agcgcgatcg cactcaaaat cgcgagcagc ccgatggtga cgagcatctc gatcagggtg
     1201 aagccgccgg atttcccctc catgcggcga actatagtcc ggccgcgcgg atttgcgcgc
     1261 atgcgggcga cgaacggtcg ttcggggcgg acgaacggtg cgcgcggcgg gaaagtaccg
     1321 cccgcggcgc ggctacggcc gggtttgcgc ctccaccgcc tcgccgctcg cggaggcgag
     1381 cggaatctcg atcgcgcagt gcaggccgcc gggaagatag tcgatccgca cttcgcccgc
     1441 gagttcgtgg gtgagtccgg cctgaatgag acgcgtgccg aagccgcggc gatgcggctt
     1501 cgcgacctcg ggcccgccgg attccttcca ttcgagccgg agtccgcgtc ccccatcgag
     1561 aagcgcggag tcgatccgca cccgccccgc gggatcgctc caggcgccgt gtcggctggc
     1621 gttggtggcg agttcgtgca gagccatgcc gagggcgagc gccgccttcg ggcgtagcag
     1681 cgcatcgtcg cccgcgatcg cgagccgccc gtcgccgtcc tcgccgaaag gcgcgagttc
     1741 gcgcgcgagg agttcgcgca ggccgacgtc gccccagcgc gcgctcgtga ggagattgtg
     1801 cgtctgcgcg agggcgagca gtcgtccctc gaaggtcttc gccacctccg tcgcaagctg
     1861 cgagcctgcg agcgtctgga tcgccagcga ctgcaccgtc gcgagcgtgt ttttcacccg
     1921 atggttgagt tcgttgacga gaagctggcg atgctcttcg gtctgcttgc gccggctgat
     1981 gtcgcggctc tccgcgacga tgagcgccgc cgcgcccggc tcttcgctca ccggatggag
     2041 cgagaagtcg aaggtgagaa cctcgcccgc ggggcgcgcg atcgcaagct cgtagcgcac
     2101 gaactcgccg gcggcggcgc gggcgagcgc cttgcgcagt tcttccgccg cgccgggcgt
     2161 gcccgcgaac cacggcgtct ccgggtacgg gcgtccgatc aagtcttcgc gcgtccaggg
     2221 accggaatcg agggccgcgc gattggcgtc gaggaccctg ccctcggaat tcagcagaac
     2281 gatgtactcg taggtgccgt cgaaaatggc gcgcagccgc gcctcgctcg catgcagcgt
     2341 ttcgtgcgcc tcgagacgct cgagaaaatc cgccgcctgg cgcgcgagca cgtcgaagag
     2401 gtcgagttcg tcctcgctcg gccagtgagg ctcgccccag tgcgtggaga gcatgccgag
     2461 cagccgaccg ccgcgcgcga cgagcggcgt ggattgcagc gcgcgaatgc ccgtgcggcg
     2521 atacagctcc agatccgggc tgcctgcgac ggagacttcg gcctcgacat cgccgacgac
     2581 gacccgccgg ccgctgcgca gcgcctgcgc acaggtgctg gccccgtcgg gtccgacgcg
     2641 catccagaac tctgcggccc cggggatgaa tccccgctgc gcaagaagcc gcagttcgcc
     2701 gctcccggga tcgaggctct ggatgctcgc gaaatcggca
//
LOCUS       DASUJG010000163        11760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_171449, whole genome shotgun sequence.
ACCESSION   DASUJG010000163 DASUJG010000000
VERSION     DASUJG010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 11760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11760
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171449"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="VFX38_03390"
     CDS             <1..892
                     /locus_tag="VFX38_03390"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HEX5313937.1"
                     /translation="VVLLKVFYPIVWVPNGISNALLRLAGLRPQTVEEARISRDELRT
                     LVLHSSALVARRQQMLLGVLELEDARVEDVMVPRSRVEGINISADWDTILAQLRASRH
                     GHLPLYRERIEDTVAMLYLRDLITPLTEGTLDAAKLRALAREPQFIPEGTSLARQLVN
                     FQRENLRCVLVVDEYGEILGLVTPKDLLQEVAGGLGEAGGEGRGDIVQQADGSLLVPG
                     YVGVRTLNRRLGWRLPVNGPRTLNGLVMEKLEDLPSKGVQLTLGGHRAEIVDTRASGT
                     VLVRFDPPRPAAIAKEPAAT"
     gene            889..1905
                     /gene="trpS"
                     /locus_tag="VFX38_03395"
     CDS             889..1905
                     /gene="trpS"
                     /locus_tag="VFX38_03395"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017366191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX5313938.1"
                     /translation="MNRKPTLVTGIKTTGTSHLGNLAGAIRPAIEASRDPGVRCYYFL
                     ADYHALINVWDPAALARSTLGIAATWLAFGLDPERVVFYRQSDVPEILELAWMLSCLT
                     AKGLMNRAHAYKAAVADNEAAGLADDPDRGVTLGLYNYPVLMAADILMFRGERVPVGR
                     DQLQHLEMTRDIAARFNHHFGEHLVLPEALIREDTAVIAGLDGRKMSKSYGNIIPLFC
                     SREELRKLVFRIKTDSTPPEAPKDPEASTLFQLYRAFATPEETGALAGEYASGIGWGA
                     VKERLFEYLDALLAVPRERYRELTAKPQTIEKTLRAGAEKARAEAGPYLAQLREAVGL
                     RPLV"
     gene            complement(2101..2943)
                     /locus_tag="VFX38_03400"
     CDS             complement(2101..2943)
                     /locus_tag="VFX38_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HEX5313939.1"
                     /translation="MSGRELDSGLKLDLAGKLTYGGYLQLERLLSAQVVRSRPEHHDE
                     MLFIVQHHVAELWMKLAIHELQWACARVAADALRPAFKALARVKLVQEQLIAEWSVLE
                     TLTPYEYQQFRPVLENASGFQSYQNRALEFLLNNKEPRLLASQRHDPKVYAWLEEILR
                     APSIYDEFLRYLARKGYAVPQERLERDFSEPYESHPGVTAVLREIYANADAAFDDYEM
                     CEKLMDVEVNLRRWRFRHLRTVERIIGHKSGTGGSSGVAYLASVIDVRCFPELTDVRT
                     ELGS"
     gene            3084..4163
                     /gene="pdhA"
                     /locus_tag="VFX38_03405"
     CDS             3084..4163
                     /gene="pdhA"
                     /locus_tag="VFX38_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEX5313940.1"
                     /translation="MAVVARFEIRYEQILDPEGKLVAKRLPDFARDPEELLRMYRKMT
                     LTRVFDGRAIALQRTGKLGTYASCLGHEATHLGVGAAMRPEDVLAPSYREYGAQFWRG
                     VKPEEVLLYWGGSELGNDFAGPKQDFAWSVPISTQNLYAAGAALAFQRRGEKRAAVAF
                     AGDGGTSEGAWYEALNAAGVWKLPMVFVIVNNRWAISVPLASQTGCETLAQKAFAGGV
                     ETLQVDGNDVIAVRKVTEEALQRAREGKGPTVIEALTYRLSDHTTADDARRYRGEKEV
                     AEAQAKEPLLRLRKYLEGLGVWDQKKESALLAECKKEIEAAVESYQKTPRQPVTAMFD
                     YMYAELPEALRAQREAAAGEESAHG"
     gene            4156..5139
                     /locus_tag="VFX38_03410"
     CDS             4156..5139
                     /locus_tag="VFX38_03410"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017334211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX5313941.1"
                     /translation="MAKITLVEAVTQALAWELEHDDSVLVFGEDVGANGGVFRATEGL
                     QKRFGAERVFDTPLAEALIAGLCVGASAQGMKPVGEIQFSGFMYPSFDQLISHASRMR
                     NRTRGRLSCPMVLRAPYGGGIHAPEHHSESMEAIYAHTPGLRVVIPSSPQRAYGLLLA
                     SIRDPDPVVFMEPKRIYRITKQDVPDDGEALPLDTCFVLREGGDLTFVSWGAMMNETL
                     KAAAELAKDGIEAEVIDLATIKPYDAETILESVERTGRLVIVAEEPRTCGVAAEIAAE
                     VAEKALYSLVAPIERVTGYDTVMPLFQLENHYLPSVARVLAAARRALEATR"
     gene            5136..6389
                     /locus_tag="VFX38_03415"
     CDS             5136..6389
                     /locus_tag="VFX38_03415"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008437914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX5313942.1"
                     /translation="MKTFDLPDLGEGLADAEIVTWHVKEGDRIETGDNLVSVETAKAV
                     VDVPSPDSVRIAKLHAAEGDVVEVHKPLVDFDVTADGKEAKPAAAARPHGGATAEEAE
                     KTEAVREDSGTVVGAMPAGEEDLEERAIIRKRGRGGEARPKAAPAARARARELGVDLG
                     SVEATGHRGQITPGDVERHASAAPSAAPAATPRPAVSAPPPRPAASGKPEAIRGPRRT
                     MAQSMSAARDQVMPTSLFDDADIHAWAPGQDITVRLIRALVAGCRAEPILNAWFDGEA
                     MTRTIHKQVDLALAVDTPDGLIVPVIRAVESLSGEVLRHELDRVKQATRARSVSPQDM
                     LNPTITLSNFGMMAGRYASPVIVPPQVAIVGTGGIRHDVVAVLGGIEVHKRIPLSLTF
                     DHRCATGGEACRYLAALIKDLERAN"
     gene            6543..11402
                     /locus_tag="VFX38_03420"
     CDS             6543..11402
                     /locus_tag="VFX38_03420"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF016944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HEX5313943.1"
                     /translation="MAARKTDAQQRDDILSRIYAAANARKSDHDHAFLKEYLHSYYGE
                     ASTGDLREYTPGNLAAAALSHLELGEHLGSKAFAVRVLNPRPKEDGWHSVHTVFQTVV
                     ADMPFLVDSTTMVAQKHGLTVHQTIHPVLAVRRDAEGRIEAIGPRGSLDGARDESFIH
                     LEVDCVSGSERLERLRHDLELALADASTAFADWTAMRQQAEALAYKMDPESSPLEPEE
                     LEEGRNFLHWLVDNRFVFLGFREYRLERQNQRDVLCVVPKSGLGILREEVDRRGTQPL
                     VLSGEARKRALTKELVIITKANSRSTVHRLAYLDYIGFKLFDANGEVCGEARFLGLFT
                     SQVYAHSPREIPIVRRKIQQVLTRSRLAPTSHAGKGLVQALETLPRDELFQASEEELF
                     TLASGILDIQERLRVRVFTRRDAFGRFYSCLIYVPRERYDAEVQQRIETIIAEAFEAR
                     EIETFVQMGESLLARVHIIAHTKAWQRARFGEKRLEQELSEATRNWDDRLQLALRKRY
                     GEDRGQALADRWCGHFPLGYQADVAAEQAVHDVRLMQRLGGARPLELKLYRSGDDAPD
                     VLRMKVFSPEQPLIASDILPLLENMGLKIISERPYEIELGDASIYWVHDFYTLIRAGT
                     SPDLGEIGPRFREAFLALWGQEAENDDLNALILSAGLDWREVRIIRAYAKYLLQTGMP
                     FGEGYLERAVAARPATARALVRYFEIRFDPQAEEAGRKERMEQAGAALDRELESLSSQ
                     DEDRILRGMAATMRATLRTNHFRRDGRGRPRHFLSFKLDSAGVPELPLPRPLYEIFVY
                     APHVEGIHLRGGKVARGGIRWSDRRADFRTEVLGLMKAQMVKNTVIVPVGCKGGFIVK
                     QPPATGNRDAVLTEVKRCYRDFIRGLLDITDNLDANRVVPPPQVLRYDEDDPYFVVAA
                     DKGTATFSDLANEIAADYDFWLGDAFASGGSVGYDHKKLGITAKGAWESVKRLFRETG
                     VNPETAEITAVGIGDMSGDVFGNGMLRSRTIRLVAAFNHLHIFIDPNPDAAASFRERE
                     RLFELPRSGWNDYDPKAISRGGGVFDRSAKSIRLSREAQEALAITGASLTPQELIRAI
                     LKAPVDLLWNGGIGTYVKSSRESQADAGDRSNDAVRVDGKDLRCKLVGEGGNLGFTQL
                     GRIEFARHGGRITTDFIDNSAGVDCSDHEVNIKILLNLALQRRSLREEERRELLVSMT
                     DQVEHLVLRDNYLQSLALSVAEAQSQQNLNEHAHLIRALERQGTLNRQIEYLPSDEEL
                     AERRVSQSGLTRPELAVLLAYGKIAVYNALERAPLAGDPYLRRELADYFPTQLRERYT
                     ELMPEHPLQGDIIATEITNSMINRMGPNFPFRIQEQAGVGMNQVARAYTISRESLNVL
                     DLWRGIEALGSDLPGQVQSSVILATGHLISHATRWVIEHGYATAAIGETVAAFGAVTR
                     ELHANLPDILPQELAARYEELRKEYADCGLSNPLARRVAALPALYAALDIAEVVRATH
                     LGVADAGRAYFRLGAELGLDWLRGQVDTLPTDDHWKALARATLREGLYAQQRRLSISA
                     LNGKAATPMVDTDARIDAWLERESANVERFRAMWTEIRATNQTEFASMSVALQKLVSL
                     ARSMGGERS"
     gene            complement(11439..>11760)
                     /locus_tag="VFX38_03425"
     CDS             complement(11439..>11760)
                     /locus_tag="VFX38_03425"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX5313944.1"
                     /translation="QGQVQILVNMIDFGMNVQEAGDAARWKHVGSPTPTGRPGQGTGT
                     VLFESGFDPALPAALEKRGYKVEMVTPGSGAFGGYEAIRYDAKNHVYWGASEMRKDGE
                     VIGY"
BASE COUNT         2122 a         4035 c         3745 g         1858 t
ORIGIN      
        1 cgtcgtgctg ctcaaagtct tctacccgat cgtctgggtt cccaacggaa tttccaacgc
       61 gctgctgcgc ctcgcgggcc tgcgcccgca gacggtggag gaggcacgca tcagccgcga
      121 cgaactgcgc acgctggtgc tccattcctc cgcgctcgtc gcgcgccgcc agcagatgct
      181 gctcggcgtg ctcgaactcg aagacgcgcg cgtggaagac gtgatggtgc cgcgcagccg
      241 cgtcgaggga atcaacatca gcgccgattg ggacacgatt ctcgcgcaac tgcgcgcgag
      301 ccgccacgga catcttccgc tctatcgcga gcgcatcgaa gacacggtcg caatgctcta
      361 tctgcgcgac ctgatcacgc cgctcaccga aggaacgctc gatgcggcga agctccgcgc
      421 actcgcgcgc gaaccgcaat tcattcccga aggcacgagt ctcgcgcggc agttggtaaa
      481 cttccagcgc gaaaatctgc gctgcgtgct cgtggtggac gaatacggcg agattctcgg
      541 tctcgtcact cccaaggatc tcctgcagga agtggcgggc ggcctcggcg aggccggcgg
      601 cgagggccgc ggcgacatcg tccaacaagc cgacgggagc ctcctcgttc cgggttacgt
      661 cggcgtgcgc acgctcaacc gccggctcgg ctggcgcctc ccggtgaacg ggccgcgcac
      721 gctcaacgga ctggtgatgg agaagctcga agacttgccg agcaagggcg tgcaactcac
      781 gctcggcgga catcgcgcgg aaatcgtcga cacgcgggcg agcggcacgg tcctcgtgcg
      841 tttcgatccg ccccgacccg cggccattgc gaaggagccc gcagcaacat gaaccgcaaa
      901 ccgacgctcg tgaccggcat caagaccacg ggcacgtcgc atctgggaaa cctcgcgggc
      961 gcgatccgtc ccgcgatcga ggcgagccgc gatcccggcg tgcgctgcta ctacttcctc
     1021 gccgactatc acgcactcat caacgtctgg gatcccgctg cgctcgcgcg ctcgaccctc
     1081 gggatcgccg cgacctggct cgccttcggg ctcgatcccg aacgggtggt tttctatcgc
     1141 caatcggacg tacccgagat tctcgaactc gcctggatgc tgtcctgcct cacggccaag
     1201 ggtctcatga accgcgcgca cgcctacaag gcggcggtcg ccgataacga agccgccgga
     1261 ctcgcggacg atcccgatcg cggcgtgacg ctcgggcttt acaactatcc ggtgctgatg
     1321 gccgccgaca tcctgatgtt tcgcggcgaa cgcgtacccg tcggccgcga tcagttgcag
     1381 catctggaga tgacccggga catcgcagca cgcttcaatc atcacttcgg ggagcatctg
     1441 gtgttgcccg aggcgctgat tcgcgaggac accgcggtga tcgccggtct cgacggacgc
     1501 aagatgagca agagctacgg caacatcatt ccgctgttct gttcgcgcga ggagttgcgc
     1561 aaactcgtct tccgcatcaa gaccgactcg acgccgcccg aggcgcccaa ggatcccgaa
     1621 gcctcgacgc tctttcagct ttatcgtgcc ttcgcgacgc cggaagaaac cggggcgctt
     1681 gcgggcgaat acgcgagcgg catcggttgg ggcgcggtca aggagcgcct cttcgaatat
     1741 ctcgacgctc tgctcgcagt cccgcgcgaa cggtaccgcg aactcaccgc caagccgcaa
     1801 acgatcgaga aaacgctgcg cgcgggcgcc gaaaaggccc gcgccgaagc cggaccgtac
     1861 ctcgcgcaac tgcgcgaggc cgtcggcctg cgccccctgg tctgatcctc gtgagggaaa
     1921 cttgagcttc acctccttcg gaacgaaggg ggtcgcgctc ttgcgcgggg ggatgttcgg
     1981 cgccgaaaga catcgccctg aatccccctt cgtaaacgaa gggggaaaag gaacgacgtt
     2041 gcgcttcgct tcgctccact caacctgtgt tgtcgagaaa gtgcggacgc gcgtgagacg
     2101 tcagctcccg agttcggtgc gcacgtcggt gagttcggga aaacagcgaa cgtcgatcac
     2161 gctcgcgaga taggccacgc cggaggagcc tcccgttccg gacttgtggc cgatgatacg
     2221 ctcgaccgtg cgcagatggc ggaagcgcca gcgccgcagg ttcacctcga cgtccatcag
     2281 cttttcgcac atttcgtagt cgtcgaaggc ggcatcggcg ttggcataga tttcgcgcag
     2341 caccgcggtc acgcccggat gcgattcgta gggctcggag aaatcgcgct cgagacgctc
     2401 ctgcggaacc gcatagccct tgcgcgcgag gtagcgcagg aattcgtcgt aaatggaggg
     2461 cgcccgcaga atctcctcga gccaggcgta cactttcgga tcatgccgct gggaggcgag
     2521 cagccgcggt tccttgttgt tcagcagaaa ctcgagcgcg cggttctggt aggactggaa
     2581 acccgaggcg ttttcgagca cggggcggaa ctgctggtat tcgtagggcg tgagcgtttc
     2641 gagcaccgac cattccgcaa tcaattgctc ctggacgagt ttgacgcgcg cgagcgcctt
     2701 gaacgccgga cgcagggcat cggccgctac ccgcgcgcag gcccattgca attcgtggat
     2761 cgcgagcttc atccacaact ccgcgacgtg atgctggacg atgaagagca tttcgtcgtg
     2821 gtgctcgggg cggctgcgca ctacctgcgc ggaaagcagc cgttcgagct ggagatagcc
     2881 gccgtaggtg agtttgccgg ccagatcgag cttgaggccg ctgtcgagtt cccgtccgct
     2941 catcgtcatg ctcccgccgt tcgagggcaa gataccacgg cgccgcgggc ccgcggccgc
     3001 ggccttccct tataatctgc ctgcaaacgg ctagcccgcg cgcgcggcgc agccacaaat
     3061 ccctccaaag gagacgccgc gtcatggccg ttgtcgcccg cttcgaaatc cgctatgagc
     3121 agatcctcga ccccgaaggc aagctcgtcg cgaagcgcct ccccgacttc gcccgcgatc
     3181 ccgaggagct gctgcgcatg tatcgcaaga tgacgctcac ccgcgttttc gacgggcggg
     3241 cgatcgcgct gcagcgcacc ggcaagctcg gcacctacgc ctcctgtctc ggccacgagg
     3301 cgacgcatct cggggtcggc gccgcgatga gacccgaaga cgtgctcgcc ccttcctacc
     3361 gcgaatacgg cgcccagttc tggcgcggcg tgaagcccga ggaggtgttg ctctactggg
     3421 gcggaagcga actcggcaac gactttgccg gaccgaagca ggacttcgcc tggtcggttc
     3481 ccatctcgac ccagaatctc tacgcggccg gcgccgcgct cgccttccag cggcgcggcg
     3541 aaaaacgcgc ggcggtcgcc ttcgccggcg acggcggcac ctccgagggc gcctggtacg
     3601 aggcgctcaa cgcggcgggt gtgtggaaac ttccgatggt cttcgtgatc gtgaacaacc
     3661 gctgggcgat ctcggtaccg ctcgcgagtc agacgggctg cgaaacgctc gcgcagaagg
     3721 ccttcgccgg cggcgtcgag accctgcagg tggacggcaa cgacgtgatc gccgtgcgca
     3781 aggttaccga agaggccttg caacgcgccc gcgaaggcaa ggggccgacg gtgatcgaag
     3841 ctctgacgta tcggctttcc gatcacacca cggccgacga tgcgcgccgc taccgcggcg
     3901 aaaaggaagt cgcggaggcg caggcgaaag aaccgctgct gcgcctgcgg aaatacctcg
     3961 aaggtctcgg ggtctgggat cagaagaagg agtcggcgct actcgccgaa tgcaagaagg
     4021 aaatcgaagc ggccgtggag agctatcaga aaacgccgcg ccaacccgtg acggcgatgt
     4081 tcgattacat gtacgccgaa ctgccggagg ccttgcgcgc gcagcgcgaa gcggccgccg
     4141 gcgaggagag cgcgcatggc taagatcacg ctcgtcgagg cggtgacgca ggcgctcgcc
     4201 tgggaactgg aacacgacga ctcggtactc gtcttcggcg aagacgtggg tgcaaacggc
     4261 ggcgtctttc gcgcgaccga gggtttgcag aaacgcttcg gcgccgagcg cgtcttcgac
     4321 accccgctcg ccgaagcgct catcgcggga ctgtgcgtcg gcgcctccgc ccagggcatg
     4381 aagccggtcg gtgagatcca gttcagcggc ttcatgtatc cctccttcga ccagttgatc
     4441 agtcacgcct cgcgcatgcg caaccgcacg cgcgggcggc tcagctgtcc gatggtgctg
     4501 cgcgcgcctt acggcggcgg catccatgcg ccggaacatc attccgaaag catggaggcg
     4561 atttacgcgc acacgccggg gctgcgggtc gtcatcccgt cttcgccgca gcgcgcctac
     4621 ggtctcctgc tcgcatcgat ccgcgatccc gacccggtcg tgttcatgga acccaaacgc
     4681 atctaccgca tcaccaagca ggacgtacca gacgacggcg aggcgctgcc gctcgacacc
     4741 tgcttcgtgc tgcgcgaagg cggcgatctc accttcgtgt cctggggcgc gatgatgaac
     4801 gaaacgctca aggcggctgc cgaactcgcg aaagacggca tcgaggccga agtcatcgat
     4861 ctggcgacca tcaagcccta cgatgcggaa acgattctgg aatcggtcga gcgcaccggg
     4921 cgtctggtga tcgtcgccga ggagccgcgg acctgcgggg ttgccgcgga aatcgcggcc
     4981 gaggttgccg agaaggcgct ttattccctg gtcgcgccga tcgagcgcgt gaccgggtac
     5041 gatacggtga tgccgctctt ccagctcgaa aatcactacc tgccctcggt cgcgcgcgtt
     5101 cttgccgcgg cgcggcgagc cctggaggcg acgcgatgaa aaccttcgat cttccggatc
     5161 tcggcgaggg tctcgccgac gccgaaatcg tcacctggca cgtcaaggaa ggcgaccgga
     5221 tcgaaaccgg cgacaacctc gtttcggtcg agaccgcgaa ggcggtcgtc gacgttccct
     5281 cccccgacag cgtacgcatc gcgaaactcc acgccgccga gggcgacgtg gtcgaggtgc
     5341 acaagccgct ggtggacttc gacgtgacag ccgacggcaa ggaagcgaag cccgccgccg
     5401 cggccaggcc ccacggcgga gccacggccg aagaggcgga aaaaaccgaa gccgtgcgcg
     5461 aggacagcgg caccgtcgtc ggcgcgatgc cggcgggcga agaagatctt gaggagcgtg
     5521 cgatcatccg caagcgcggc cgcggcggcg aagcccgccc caaggcggcg ccggcggcgc
     5581 gggcgcgggc ccgcgaactc ggagtcgatc tcggctcggt cgaggccacc gggcatcgcg
     5641 ggcagattac cccgggcgac gtcgaacggc acgcgagcgc ggcgccgagc gccgccccgg
     5701 ccgcaacgcc tcgccccgcg gtttccgctc cgccgccgcg accggcggct tccggcaaac
     5761 ccgaggcgat ccgcggaccg cgccgcacca tggcgcagag catgagcgcc gcccgcgatc
     5821 aggtcatgcc gaccagcctc ttcgacgacg ccgacattca cgcctgggca ccgggccagg
     5881 acatcaccgt gcgcctgatc cgggcgctgg tcgccggctg ccgcgccgag cccatcctca
     5941 acgcctggtt cgatggcgag gcgatgaccc gcaccatcca caagcaggtg gatctcgcgc
     6001 tcgctgtgga cacccccgac gggctcatcg tgccggtgat tcgcgccgtc gagtccctga
     6061 gcggcgaggt gctccgccac gaactcgacc gcgtcaaaca agccacgcgg gcgcgcagcg
     6121 tgagccccca ggacatgctc aaccccacca tcacgctgtc gaacttcggg atgatggccg
     6181 ggcgctacgc gagccccgtg atcgtgccgc cgcaggtcgc gatcgtcggc accggcggca
     6241 tccgtcacga cgtcgtcgcg gtactcggcg gcatcgaggt acacaaacgc attccgctgt
     6301 ccctgacctt cgatcatcgc tgcgcaaccg gcggagaagc ctgccgctat ctcgcggccc
     6361 tcatcaagga cctcgaacgc gccaactgag aagggccgcc cggccgcaca ccggtttttc
     6421 ttcccctgtc cccgacctgc gcaatgccgg cacggcccgg aaatgaaaag cgccaccgaa
     6481 gcatgggcgc ggcgaagcgg ctaaaataaa cggttcccgc gtttccaggg cgcgaccctc
     6541 tcatggcggc cagaaaaacc gatgcgcagc agcgcgacga catcctttcg aggatctacg
     6601 ccgctgccaa tgccaggaag agcgatcacg atcacgcctt tctgaaggaa tacctgcaca
     6661 gctattacgg cgaggcaagc accggcgatc tgcgcgaata cacgcccggc aacctcgcgg
     6721 ccgccgcgct ctcgcatctc gaactgggcg agcatctcgg cagtaaagcc ttcgcagtgc
     6781 gcgtgctcaa tccccgcccg aaggaggacg gctggcattc ggtgcatacg gtgttccaga
     6841 ccgtcgtcgc cgacatgccc tttctcgtcg attccaccac gatggtggcg cagaagcacg
     6901 ggctcacggt gcaccagacg atccatccgg tgctcgcggt gcgccgcgac gcggaaggcc
     6961 gcatcgaggc gatcgggccg cgcggcagcc tcgatggcgc tcgcgacgag tccttcatcc
     7021 acctcgaagt ggactgcgtc agcggcagcg aacgcctcga gcggctccgc cacgatctcg
     7081 aactcgcgct cgccgatgcc tcgaccgcat tcgccgactg gacggcgatg cgccaacagg
     7141 ccgaagccct cgcctacaag atggacccgg aaagctcgcc gctcgaaccc gaggaactcg
     7201 aggagggccg caacttcctc cactggctgg tagacaaccg cttcgtcttt ctcggctttc
     7261 gcgagtatcg gctcgaaagg cagaaccagc gcgacgtgct ctgcgtcgtg ccgaagagcg
     7321 gcctcggcat cctgcgcgaa gaagtcgacc ggcgcgggac gcaaccgctc gtcctctccg
     7381 gcgaggcgcg caagcgggcg ctcaccaagg aactcgtcat catcaccaag gcgaattcgc
     7441 gctcgacggt gcaccgcctc gcctatctcg actacatcgg cttcaagctg ttcgacgcca
     7501 acggtgaggt ctgcggcgag gcccgcttcc tcggcctttt cacctcgcag gtctacgccc
     7561 acagcccgcg cgagattccg atcgtgcggc gcaagattca gcaggtgctc acgcgctcgc
     7621 gtcttgcgcc gacgagccac gccggcaagg gtctggtgca ggcgctcgaa accttgccgc
     7681 gcgacgaact ctttcaggcg agcgaagaag agctcttcac gctcgcgagc ggcattctcg
     7741 acatccagga gcgtctgcgc gtgcgggtgt tcacgcggcg cgacgccttc gggcgcttct
     7801 attcctgcct gatctacgtt ccgcgcgagc gttacgacgc cgaggtgcag cagcgcatcg
     7861 agacgatcat cgccgaggcc ttcgaggcgc gcgaaatcga gaccttcgtg cagatgggcg
     7921 aatcgctcct cgcgcgggtg cacatcatcg cccacaccaa ggcctggcag cgcgcccgct
     7981 tcggcgaaaa gcgcctcgaa caggaacttt ccgaggcgac ccgcaactgg gacgatcgcc
     8041 tgcagctcgc gctgcgcaaa cgctacggag aagaccgcgg tcaggcgctt gccgatcgct
     8101 ggtgcggcca cttcccgctc ggctaccagg ccgacgtcgc tgccgaacag gccgtgcacg
     8161 acgtgcgcct gatgcagcgg ctcggcggcg cgcgcccgct cgagctgaag ctctatcgca
     8221 gcggcgacga tgccccggat gtcctgcgca tgaaggtgtt ttcgccggag cagccgctca
     8281 tcgcctccga cattctcccg ctcctcgaga acatgggcct gaagatcatc tccgagcgcc
     8341 cttacgaaat cgaactcggc gatgcctcga tctactgggt gcatgatttc tacaccctca
     8401 tccgcgcggg aacgagtccc gatctcggcg aaatcggccc gcgctttcgc gaggcctttc
     8461 tcgcgctctg ggggcaggaa gcggaaaacg acgatctcaa tgcgctcatt ctcagcgccg
     8521 gactcgactg gcgcgaggtg cgcatcatcc gcgcctacgc gaagtatctt ctgcaaacgg
     8581 gcatgccctt cggcgaaggc tatctcgagc gcgccgtcgc ggcgcggccg gcgaccgccc
     8641 gcgcgctggt gcggtacttc gaaatccgtt tcgatccgca ggccgaagaa gcgggacgca
     8701 aggaacgcat ggagcaggcg ggcgcggcgc tcgacaggga actcgaaagc ctgagcagtc
     8761 aggacgagga ccggatcctc cgcggaatgg cggcgaccat gcgggcgacc ttgcgcacca
     8821 atcacttccg acgcgacggc cgcggccgcc cgcgccattt cctctcgttc aagctcgatt
     8881 cggccggcgt gccggaactg ccgctcccgc gcccgctgta cgaaatcttc gtgtacgcgc
     8941 cgcatgtgga gggcatccat ttgcgcggcg gcaaggtcgc acgcggcggc atccgctggt
     9001 cggaccggcg cgcggatttc cgcaccgaag tgctcggcct gatgaaggcg cagatggtga
     9061 agaacaccgt catcgtgccg gtcggctgca agggcggctt catcgtcaag caaccgcccg
     9121 cgaccggcaa tcgcgacgcg gtgctgacgg aggtcaaacg ctgctatcgc gacttcatcc
     9181 gcgggctcct cgacatcacc gacaatctcg acgcgaaccg cgtcgtgccg ccgccgcagg
     9241 tgctgcgtta cgacgaggac gatccctact tcgtggtcgc cgccgacaag ggcacggcga
     9301 ccttctccga tctcgcgaac gagatcgcgg ccgactacga cttctggctc ggcgatgcct
     9361 tcgcttccgg cggctcggtc ggctacgacc acaagaagct cggcatcacc gccaagggcg
     9421 cctgggaatc ggtcaagcgc ctcttccgcg aaacgggggt gaacccggaa acggccgaaa
     9481 tcaccgcggt cggcatcggc gacatgtcgg gcgacgtgtt cggaaacggc atgctgcgtt
     9541 cgcgcacgat ccgtctcgtc gcggcgttca atcatcttca catcttcatc gacccgaacc
     9601 cggacgcggc ggcgagtttc cgcgaacgcg agcgcctgtt cgaactgccg cgttcgggct
     9661 ggaacgatta cgatccgaag gcgatttcgc gcggcggcgg cgtcttcgat cgcagcgcga
     9721 aatccattcg cctgagccgc gaagcgcagg aggcgctcgc gatcacgggc gcaagcctca
     9781 ccccgcagga actcatccgc gccatcctca aggcgcccgt cgatctgctc tggaacggcg
     9841 gcatcggcac ctacgtgaaa tcgagccgcg agagccaggc cgacgccggc gatcgcagca
     9901 acgatgcggt gcgtgtcgac ggcaaggatc tgcgctgcaa gctcgtcggc gaaggcggca
     9961 atctcggctt cactcaactc ggacgcatcg aatttgcgcg ccacggcggg cggatcacga
    10021 ccgacttcat cgacaattcg gccggcgtgg actgctcgga tcatgaagtc aacatcaaga
    10081 tcctgctcaa cctcgcgctc cagcggcgga gtctgcgcga agaagagcga cgcgaactgc
    10141 tcgtgagcat gaccgaccag gtcgagcacc tcgtgctccg cgacaactat ctgcagagcc
    10201 tcgcgctttc ggttgccgag gcgcagtcgc aacaaaacct gaacgagcat gcgcatctca
    10261 tccgcgcgct cgagcgtcag ggtacgctca accgccagat cgagtatctc ccgagcgacg
    10321 aggaactcgc ggaacggcgg gtgagccaga gcggactcac gcgcccggaa ctcgcggttc
    10381 tgctcgccta cggcaagatc gcagtctaca acgcactcga gcgggcgccg ctcgccgggg
    10441 atccctacct gcgccgggag ctcgccgatt acttccccac gcaacttcgc gagcgttaca
    10501 cggaactcat gcccgagcac ccgctgcagg gggacatcat cgcgaccgag atcaccaaca
    10561 gcatgatcaa ccgcatgggg ccgaatttcc cgttccgcat tcaggaacag gcgggcgtgg
    10621 gcatgaacca ggtcgcccgc gcctacacca tctcgcgcga atcgctcaac gtgctcgatc
    10681 tgtggcgcgg aatcgaggca ctcggctccg atctaccggg acaggtgcag agttcggtga
    10741 tcctcgcgac cgggcatctc atcagccacg ccacgcgctg ggtgatcgag cacggatacg
    10801 cgacggccgc gatcggcgaa acggtcgcgg ccttcggggc cgtcacccgc gaactccatg
    10861 ccaatcttcc cgatattctt ccgcaggaac tcgcggcacg ctacgaggag ctgcgcaagg
    10921 aatacgccga ttgcggcctg agcaatccgc tcgcccgccg ggtggcggcg ctgcccgcgc
    10981 tctacgccgc gctcgacatc gccgaagtcg tacgtgcgac ccacctcggc gtcgccgacg
    11041 caggccgcgc gtatttccgc ctcggtgccg aactcggact cgactggctg cgcgggcagg
    11101 tcgacacgct cccgaccgac gatcactgga aggcgctcgc gcgagcgacg ctgcgagaag
    11161 gcctgtacgc acaacagcgc cgcctgagca tttcggccct gaacggcaag gccgcgacgc
    11221 ccatggtcga taccgacgcg cgcatcgacg cctggctcga gcgcgagtca gccaacgtcg
    11281 agcgtttccg cgccatgtgg acggagattc gcgccaccaa tcagacggag ttcgcctcga
    11341 tgtcggtggc gctgcaaaaa ctcgtctcgc tcgcgcgaag catgggcggc gagcgaagct
    11401 gaggcgtcgc ggcgctccga cttcgcgcga cacgtccgtc aatagccgat gacttcgccg
    11461 tccttgcgca tctcgctcgc gccccaatag acgtgattct tcgcgtcgta ccggatcgcc
    11521 tcgtagcctc cgaacgcgcc gctccccggc gttaccatct ccaccttgta tccgcgcttt
    11581 tccagcgcgg ccggtagcgc cgggtcgaag cccgattcga agagcacggt acccgtgccc
    11641 tgccccggcc ggccggtcgg ggtcgggctg ccgacatgct tccagcgcgc ggcgtcgccc
    11701 gcttcctgca cgttcatccc gaagtcgatc atgttcacga ggatttgcac ctgtccctgc
//
LOCUS       DASUJG010000164         5086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_781204, whole genome shotgun sequence.
ACCESSION   DASUJG010000164 DASUJG010000000
VERSION     DASUJG010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781204"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="VFX38_03430"
     CDS             complement(<1..764)
                     /locus_tag="VFX38_03430"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5313945.1"
                     /translation="MQALHRENNVVDFALSEEEGMIAELAARFAHEVIAPNAAHWDAK
                     GEFPADIVSQARELGLMNVSVPAEFGGTGGSVLDLILVAEQLGWGCTGIGLAILLNNL
                     AADPINVAANDEQRKKYFGMLTEGFGAYALTEPAAGSDVSAIRTRAEKKGGDWVIDGQ
                     KIWISNASVADFFVVFAKTEPGERARGITAFLVDRDTPGVAVGKPLPKMGQKASPACE
                     IFFEEVCVPDSQRLGAENQGFKVAMRVFDRSRPMVGA"
     gene            complement(790..1467)
                     /locus_tag="VFX38_03435"
     CDS             complement(790..1467)
                     /locus_tag="VFX38_03435"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HEX5313946.1"
                     /translation="MSGHADFAANDYGILSGLVPAETARLCAGYALANRNLPRYYAAE
                     AMHNSWGRYADSIGEVILAQVQPKIEELTGLALFPAYSYLRIYCPGSVLLSHIDRPSC
                     EISATLTLGGDAAQPWPIWVKSKDGPRAIELPPGDGMVYRGTVVPHWRERFEGRFWIQ
                     LFLHYVRRDGQFAQHRFDGREHLGPVAPGENRPHGDVPRQFAAEDPCPCGSGRSYASC
                     HGAVPGA"
     gene            complement(1469..2344)
                     /locus_tag="VFX38_03440"
     CDS             complement(1469..2344)
                     /locus_tag="VFX38_03440"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX5313947.1"
                     /translation="MNGCKLLSFRTLIDAEDLRRHRDGPGWCVFDCRCSPFDPAVGRS
                     AYLAGHIPGARHADLDWVLAGPKTATSGRHPLPARAVLAEWLGREGVGGASQVVAYDD
                     AGGAFAARLWWLLRWLGHERVAVLDGGLTAWCEAGGELEAAEAPSVPAAAFTPGEPLG
                     QCLDAAEVLEISRERSPGVLVDARAAPRYRGESEPVDAVAGHIPGALNRPYGGNLDAS
                     RRFLDRERLRERFAGLSRNPSLVVHYCGSGVTACHNVLAMEHAGLTGSRLYAGSWSEW
                     IRDPERPVTRGPEGE"
     gene            2396..3217
                     /locus_tag="VFX38_03445"
     CDS             2396..3217
                     /locus_tag="VFX38_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008210071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5313948.1"
                     /translation="MADLHGKTLFITGASRGIGEAIALRAARDGANIAVAAKTDSPNP
                     KLPGTIHTAAQAIEKAGGKALALRVDIRDEEAVAAAVARAAEHFGGIDILVNNASAIY
                     LARTADTPIKRLDLMFDVNMRGSFVCAQACLPFLRRASNPHILSLAPPPNLDPRWFRE
                     HVAYTLSKYGMSLAAFGMAAEFRDYGIAVNTLWPCTVIATAAIAMLGGRVTPAECRKP
                     EIVADAAHAILLRSARECSGRHFTDEKALREAGVTDFERYAVSPGTALAPDLFLD"
     gene            complement(3227..3685)
                     /locus_tag="VFX38_03450"
     CDS             complement(3227..3685)
                     /locus_tag="VFX38_03450"
                     /inference="COORDINATES: protein motif:HMM:NF025691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HEX5313949.1"
                     /translation="MADESSWTSHVLAGATWRRFAFMLIFVPILSCALFLMGFTSLFQ
                     FFSMLASGETNPQLTSFGHGLARFAAEIMDYLTYNTERRPFPFAGWQGAERSPRRRSR
                     PASERRKRKVRSSSRRRGATEPVVRLNPPSELRPGNDAQAPPEEPKRPPE"
     gene            3744..4532
                     /locus_tag="VFX38_03455"
     CDS             3744..4532
                     /locus_tag="VFX38_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX5313950.1"
                     /translation="MQSWIDIGANLTHESFELDREAVIERAGAAGVTRMIVTGADVAH
                     SRAAVALARGYPGKLYATAGVHPHAAAGFSPETSAALRGLLAEPQVVAAGECGLDYFR
                     DLAPRPAQREAFAAQIALAIECGKPLFLHQRDAHADWLAILDEFGSRLPAAVIHCFTG
                     NAAELDDCLERGFHVGITGWICDERRGRHLAELLARIPPGRLMLETDAPYLLPRDLHP
                     RPRTRRNEPQWLPHVARAVAAARRETIDELAAHTTASAVAFFGL"
     gene            4633..>5086
                     /locus_tag="VFX38_03460"
     CDS             4633..>5086
                     /locus_tag="VFX38_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5313951.1"
                     /translation="MDSGKSAQFCAEAWDEKIVPALENYIRIPAKSPSFDPDWEANGH
                     IERAAQLAADWCRKNAPARMHFEIHRLPGRTPLLFMEIAGAAPPSADAPCVLLYGHLD
                     KQPEFEGWHEGLAPWEPVIRDGRLYGRGGADDGYAVFASLTAIRALQEQ"
BASE COUNT          897 a         1779 c         1603 g          807 t
ORIGIN      
        1 gcccccacca tcggacgcga acgatcgaaa acacgcatcg ccaccttgaa gccctggttc
       61 tcggcgccga ggcgctgaga atcgggaacg caaacttcct cgaaaaaaat ttcacaggcc
      121 ggcgaagcct tctgtcccat cttcggaagc ggcttgccca ccgcgactcc cggtgtgtcg
      181 cgatcgacga gaaaggccgt gatgccgcgc gcacgctctc cgggttccgt tttggcgaac
      241 accacgaaaa agtcggcgac gctggcgttg ctgatccaga tcttctggcc gtcgatcacc
      301 caatcgccgc ccttcttctc ggcccgggtg cggatcgcac tcacgtcgga accggccgcc
      361 ggttcggtca gggcataggc tccgaaacct tccgtcagca tgccgaaata cttcttgcgt
      421 tgctcgtcgt tggccgcaac gttgatcgga tcggcggcga gattgttgag caggatggcg
      481 aggccgatgc cggtgcagcc ccagccgagt tgttcggcga cgagaatcag atcgagcacc
      541 gatccgccgg taccgccgaa ctctgcgggc acgctgacat tcatcaggcc gagttcgcgt
      601 gcctgcgaaa caatgtcggc aggaaattct cccttggcat cccaatgagc ggcgttcgga
      661 gcgatgacct cgtgtgcgaa cctggcggcc agctccgcga tcatcccctc ttcttcactc
      721 agtgcaaaat ccaccacgtt gttctctcgg tgcagggcct gcaaccgcct attctatagg
      781 cacagccgct caggcccccg gcacggcgcc gtggcaggag gcgtaggacc gtccgctgcc
      841 gcagggacag ggatcttcgg cggcgaattg acgcggcacg tcgccgtgcg ggcgattctc
      901 ccccggtgca accggcccca gatgttcgcg tccgtcgaag cgatgctgcg caaactggcc
      961 gtcgcggcgc acgtaatgca gaaacagttg aatccagaag cggccctcga aacgctcgcg
     1021 ccagtgcggc accaccgttc cgcgatacac catgccgtcg cccggcggca gctcgatggc
     1081 gcgcggtccg tccttcgatt tcacccagat cggccaaggc tgggcggcat ccccgccgag
     1141 tgtgagcgtc gcgctgattt cgcaagaggg gcgatcgatg tgcgacagga gcaccgatcc
     1201 cgggcaatag atccgcaggt acgaataggc gggaaagagc gcaagccccg tcagctcctc
     1261 gattttcggt tgcacctgcg cgaggatcac ctcgccgatg ctgtcggcgt agcggcccca
     1321 ggaattgtgc atggcttcgg ccgcgtagta gcgcggcaga ttgcgattgg cgagcgcgta
     1381 gccggcgcaa aggcgcgccg tctcggccgg gacgaggccg ctcaggatcc cgtaatcatt
     1441 tgccgcaaag tcggcatgtc cgctcatctc actccccttc gggaccgcgg gtcacgggcc
     1501 gctccggatc gcgaatccat tcgctccagg agccggcata cagacgcgaa cccgtgagcc
     1561 cggcgtgttc catcgcgagc acattatggc aggccgtcac gcccgagccg cagtagtgaa
     1621 cgaccagcga aggatttcgc gacagcccgg cgaaacgctc gcgcaggcgc tcccgatcga
     1681 gaaagcgtcg gctcgcatcg aggttcccgc catacgggcg attcaaggcg ccgggaatgt
     1741 ggcccgcgac cgcatccacg ggttcgctct cgccgcgata gcggggcgcg gcgcgggcat
     1801 ccacgagcac cccggggctg cgctcgcgcg aaatctccag gacctcggcc gcatcgagac
     1861 actgccccag gggttcgcca ggcgtaaatg ctgccgccgg caccgacggg gcctccgccg
     1921 cttcgagttc gccgcccgct tcacaccagg ccgtgagtcc gccgtctagc acggccaccc
     1981 gctcatgccc gagccagcgc aggagccacc aaagccgcgc cgcgaaggcc ccgcccgcat
     2041 cgtcataggc gacgacctga ctcgccccgc cgacgccctc gcgccccagc cattcggcga
     2101 gcacggcgcg tgcaggcaag ggatgtcggc cgctcgtcgc cgttttcggg ccggcgagca
     2161 cccagtcgag atcggcgtgg cgggcacccg ggatgtgccc ggcgaggtag gcactcctgc
     2221 ccacggcggg atcaaagggc gagcaacgac aatcgaagac gcaccagccg ggcccatcgc
     2281 ggtggcgacg aaggtcttcg gcgtcgatca aggtccggaa ggacaagagt ttgcatccgt
     2341 tcattgccta tactgtgccg gttccgcccg atcggcgcaa accgccgaat catccatggc
     2401 agacctgcac ggcaagacgc tcttcattac cggcgcgagt cgcggcatcg gcgaagcgat
     2461 tgccctgcgc gcggcccgcg acggcgccaa catcgccgtc gccgccaaaa ccgacagccc
     2521 caatcccaag ttgccgggaa cgattcatac cgccgcgcaa gccattgaaa aggcaggagg
     2581 caaggcgctg gccctgcggg tggatattcg cgacgaagag gccgtcgccg ctgcggtcgc
     2641 acgcgcggcc gaacacttcg gcggcatcga cattctggtc aacaatgcaa gcgccatcta
     2701 tcttgcgcgc accgccgaca ctccgatcaa gcgcctcgat ctgatgttcg acgtcaacat
     2761 gcgtggcagc ttcgtctgcg cgcaggcctg ccttcccttt ctccgccgcg cgtcgaatcc
     2821 gcacattctc agtctcgcgc cgccgccgaa tctcgatccg cgctggtttc gcgaacacgt
     2881 cgcctacacg ctcagcaagt acggcatgag tctcgccgcc ttcggcatgg ccgcggagtt
     2941 tcgcgactat ggcattgccg tgaacaccct ctggccctgc accgtcatcg caacggccgc
     3001 gatcgcgatg ctcggcgggc gcgtgacgcc ggccgaatgc cgcaagcccg agatcgtcgc
     3061 cgacgcggcc cacgccattc ttctgcgttc cgcccgcgaa tgcagcgggc gtcatttcac
     3121 cgacgagaaa gcattgcgcg aagccggcgt gaccgacttc gagcgctatg ccgtgagccc
     3181 gggaacggcg cttgcgcccg acctgttcct cgattgacga acgcggttat tccgggggcc
     3241 gcttcggttc ttccggcgga gcttgggcgt cgttgccggg gcgcagttcg ctcggcggat
     3301 tcaggcgcac caccggctcc gtcgcgccgc gccgtcgcga tgagctgcgc accttgcgtt
     3361 tgcgccgttc cgacgccggc cgggaacggc ggcggggcga acgctccgcg ccctgccagc
     3421 ccgcgaacgg gaacggccgc cgctcggtgt tgtaagtgag atagtccatg atttcggcgg
     3481 cgaagcgcgc gagtccatgg ccgaaactcg tgagttgcgg attggtctcc ccgctcgcga
     3541 gcatgctgaa gaactgaaac agcgaggtga agcccatgag gaacagggcg caactcaaaa
     3601 tgggaacgaa gatcagcatg aacgcgaagc ggcgccaggt ggcgccggca agaacgtggc
     3661 tggtccaact cgattcgtcc gccacccgaa attcctcgac cgtccgcggc aaagcttagc
     3721 agaggcgaga cgggcccatc ggcatgcaga gctggatcga catcggcgcc aatctcaccc
     3781 atgagagctt cgagctggat cgcgaggccg tcatcgaacg cgccggcgcg gccggcgtca
     3841 cccgcatgat cgtgaccggc gccgatgtcg cgcactcgcg ggccgccgtc gcgctcgccc
     3901 gcggatatcc cggaaaactc tacgcgaccg cgggcgtcca cccgcatgcc gcagcagggt
     3961 tttcgcccga gacaagcgcg gccctgcgcg gccttctcgc cgaaccgcaa gtcgtcgccg
     4021 ccggcgaatg tggcctcgac tacttccgcg atctcgcacc gcgccccgcc caacgcgaag
     4081 cgttcgccgc gcagatcgcg ctcgcgatcg aatgcggcaa gccgctcttc ctccaccagc
     4141 gcgacgcgca cgccgactgg ctcgcgattc tcgatgagtt cggctcgcgt ctcccggccg
     4201 ccgtaattca ttgcttcacg ggcaatgccg ccgaactcga cgactgcctc gagcgcggtt
     4261 ttcacgtcgg catcaccggc tggatctgcg acgagcgccg cggacggcat ctggcggaac
     4321 tgctcgcacg catcccgccc ggccggctca tgctcgagac cgacgcgccg tacctcctgc
     4381 cgcgggatct ccatccgcga ccgcgaacgc gccgcaacga gccgcagtgg ctgccgcatg
     4441 tcgcccgggc cgtcgccgcg gcccgacgcg aaacgatcga cgaactcgcg gcgcacacga
     4501 ccgcgagcgc cgtcgccttt ttcggcctgt agcagtcccc gaagcgccgt ttgcggatgt
     4561 gccgcagttc cttgtgcagc ctccggcagg tacaataacg cccttgcgtc cgtaccagag
     4621 gagccggcga gaatggactc gggaaagagc gcgcaattct gcgccgaggc atgggacgag
     4681 aaaattgtcc ccgcgctcga aaactacatt cgcattccgg caaagtcgcc gagcttcgat
     4741 cccgattggg aagcgaacgg acacatcgag cgcgcggcgc agctcgcggc cgactggtgc
     4801 cgcaaaaacg ctccggcgcg gatgcacttc gagattcatc gcctgccggg acgcacgccg
     4861 ctgctcttca tggagattgc gggagccgct ccgccgagcg ccgacgcacc ctgcgtcctg
     4921 ctctacggtc atctcgacaa gcaacccgaa ttcgagggct ggcacgaagg cctcgcgccc
     4981 tgggaaccgg tgattcgcga cggaaggctt tacgggcgtg gcggcgccga cgacggctat
     5041 gcggtgttcg cctcgctcac cgcgattcgc gccctgcagg agcaga
//
LOCUS       DASUJG010000165         2390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1394564, whole genome shotgun sequence.
ACCESSION   DASUJG010000165 DASUJG010000000
VERSION     DASUJG010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1394564"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VFX38_03465"
     CDS             <1..276
                     /locus_tag="VFX38_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313952.1"
                     /translation="DNTAYARLRGTVSAQPGALVMYGWPRSLVLYQLGAPMPWLRDAR
                     DVYRAVHSRRLAPGDYLLVPRDNLAAYGSHGPFTLTPAPAPPTSPAS"
     gene            273..398
                     /locus_tag="VFX38_03470"
     CDS             273..398
                     /locus_tag="VFX38_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313953.1"
                     /translation="MTLESKGGIEVYRVLPSAASAGLPPTPAPPPTPWWARFDTD"
     gene            401..1468
                     /locus_tag="VFX38_03475"
     CDS             401..1468
                     /locus_tag="VFX38_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313954.1"
                     /translation="MSPASPHRFTRAEWLEAFTGDPDVFFFRLDASSRKALLVRLDAE
                     RIREAAFLDERALKGDEDGVWLPLDALLETPASERAPQGIILHCGHCGSTLISRLLGE
                     LPATRVLREPLVLQDLAAEFRLAGSAYARLRPEELRRTLVLAGNSFAKASTSATHVIV
                     KHTSFTANLGVSLLESGLTPAVLLLWIPLEEYLATMLRDPGLRQGLRQAASQWLHDLV
                     RELGAGAPRLAELADGEVAALNWTAAQLAFARVAERSEARLLAWPFGDFLAHSETRLR
                     QLARHFALDADEAEIRKALASPWLRRYAKDPRYPFDAGIRARELDAARTRFAAEIDKG
                     CGFARGLWDRLRLEHAPGEKP"
     gene            complement(1482..>2390)
                     /gene="hemH"
                     /locus_tag="VFX38_03480"
     CDS             complement(1482..>2390)
                     /gene="hemH"
                     /locus_tag="VFX38_03480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX5313955.1"
                     /translation="AEFLADPRVIELPRALRWALLHGIVLRLRPRRTARAYRRIWRDD
                     GSPLAVFSRALGARLREVLVERTSRPITLRLAMRYGNPGVREALESLMAQGIDRLIVL
                     PLYPQYSAATTASAFDAFARAASHSRRLPSVHFVSHYHEDAHYVAAVAAAVSEAGERG
                     ERLLFSFHGMPQATRAAGDPYYDQCRESARLIAQSLGLVPDQWSTAFQSRFGRAAWLS
                     PATDAVLRAWAREGLKSVSVVCPGFAVDCLETLEEVALRYREVFLSAGGERFHYIPAL
                     NDRPDHARALGELVLRELERTPASEG"
BASE COUNT          389 a          829 c          788 g          384 t
ORIGIN      
        1 gacaataccg cctatgcccg cctccgagga accgtttccg cgcagcccgg agcactcgtg
       61 atgtacggct ggccgcgctc gctggtgctc tatcagctcg gcgcgcccat gccgtggttg
      121 cgcgatgcgc gcgacgtgta ccgggccgtc cactcgcgcc ggctcgcgcc cggcgattat
      181 ctgctcgttc cccgcgacaa tctcgcggcc tacggttccc acggcccttt cacgctcacg
      241 ccggcgccgg ccccccctac ttcacccgcg tcatgacgct cgaaagcaaa ggcggaatcg
      301 aggtctaccg ggtgttgccg agcgcggcga gcgccgggct tccaccgacg ccggcgccgc
      361 cgccgacacc gtggtgggcg cgcttcgata cggattgagg atgtcgccgg cttccccgca
      421 tcgttttacc cgcgccgagt ggttggaggc cttcacaggt gatccggatg tgtttttctt
      481 ccgtctggat gccagctccc gcaaggcgct actggtccgc cttgatgccg agcgtatccg
      541 cgaagccgcg tttctcgacg agcgggccct caagggcgac gaggacggcg tctggctgcc
      601 tctcgacgcc ctgctcgaga cgcccgcgag cgaacgcgct ccgcagggaa tcatcctcca
      661 ctgcggccat tgcggctcca ccttgatctc gcgcctgctg ggcgaactgc ccgcgacacg
      721 cgtgcttcgc gaaccactcg tgcttcagga tctcgcggcc gagttccggc tcgccggcag
      781 tgcatacgcg cggctgcgac ccgaagaact ccggcgcacg ctcgtgctcg ccgggaattc
      841 attcgccaag gcgtcgacgt ccgcgacgca cgtaatcgtc aagcacacga gttttacggc
      901 caatctcgga gtttcgttgc tcgaatcggg tctcacgccg gccgtgcttc tcctgtggat
      961 tccgctcgaa gagtatctcg cgacgatgct ccgcgacccc ggcttgcggc aggggctgcg
     1021 ccaggcggcg tcgcaatggc ttcacgatct cgtgcgggag ctcggggcgg gcgctccgcg
     1081 tctcgcggaa ctcgccgacg gcgaagttgc cgcgctcaac tggacggcgg cgcaacttgc
     1141 cttcgcccgg gtcgccgaac gaagcgaagc gcgactcctt gcctggccgt tcggcgattt
     1201 tctcgctcac tccgaaacac ggctccgcca gctcgcccgc catttcgctc tcgacgcgga
     1261 cgaagcggag attcgcaagg cgctcgcgag cccctggctt cgtcgctacg ccaaggaccc
     1321 ccgttacccc ttcgacgcgg gcatccgtgc acgggaactc gacgcggcaa ggacgcgttt
     1381 cgccgccgag atcgacaagg gatgcggctt tgcgcgcggc ttatgggacc gcctgcgcct
     1441 cgagcatgcc ccgggcgaga agccttgagc cgggatccgc tttagccttc ggaggcgggc
     1501 gtgcgttcca gctcacgcag gaccagttcg ccgagtgcgc gtgcgtgatc gggacgatca
     1561 ttgagcgccg gaatgtaatg aaagcgctcg ccgcccgcag aaagaaatac ctcccggtag
     1621 cgaagggcga cctcctccag cgtttccaga caatccacgg cgaagccggg acacacgacg
     1681 ctcaccgatt tcaaaccttc gcgcgcccag gcacgaagga ccgcgtcggt cgccggcgac
     1741 agccacgcgg cgcgaccgaa gcgcgactga aaggcggtgg accattgatc gggcacgagg
     1801 ccgagggatt gcgcaatcag ccgcgcgctt tcccggcact ggtcgtagta cggatcgccc
     1861 gcggcgcggg ttgcttgcgg catgccgtga aaggagaaaa gcagccgctc gccacgctcg
     1921 ccggcttcgg acacggccgc ggcgacggcg gcaacatagt gggcgtcttc gtgatagtgg
     1981 ctcacgaaat gcaccgacgg caggcggcgc gaatgcgacg cggcccgggc gaacgcatcg
     2041 aatgcggaag ccgtggtcgc ggcggagtac tgcggataaa gcggcagcac gatcaggcgg
     2101 tcgatgccct gcgccatgag actctccaac gcctcgcgga cgccgggatt cccgtaacgc
     2161 attgcgagcc gcagcgtaat cggtcttgac gttcgttcga cgagaacttc tcgcaaccgc
     2221 gcgcccagag cgcggctgaa cacggcgagc ggcgagccgt cgtcgcgcca gatgcggcgg
     2281 taggcgcggg cggtgcgacg cggacgcagc cgcagcacga tgccgtgcag cagcgcccag
     2341 cgcaaggcgc gaggcaattc gatcacgcgc ggatcggcga ggaactcggc
//
LOCUS       DASUJG010000166         4824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1028559, whole genome shotgun sequence.
ACCESSION   DASUJG010000166 DASUJG010000000
VERSION     DASUJG010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028559"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..1093
                     /gene="prfB"
                     /locus_tag="VFX38_03485"
     CDS             join(25..69,71..1093)
                     /gene="prfB"
                     /locus_tag="VFX38_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638028.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX5313956.1"
                     /translation="MADLESRSEALRRYLDYDGSRERLAEVERALEAPEVWSDPAQAQ
                     HLGRERVRLAGIVTTLDEVANSLRDSADLLELAAEEDDAQALAQIDSDVATSAARIEE
                     LEFRRMFAGEMDAANAFLDIQSGSGGTEAQDWSEMLLRMYLRWGERRGFATELIEVSP
                     GEVAGIKSATIRFGGDYAYGWLRTETGVHRLVRKSPFDSGHRRHTSFAAVSVSPEVDD
                     EIEIEINPAELRVDVYRASGAGGQHVNRTESAVRITHLPTGIVVQCQNERSQHKNRAT
                     AMKQLKAKLYELELSKRRSAQQEIEEAKSDIGWGSQIRSYVLDQSRIKDLRTGVEIAN
                     TQGVLDGALDPFIEAALKAGV"
     gene            1233..2744
                     /gene="lysS"
                     /locus_tag="VFX38_03490"
     CDS             1233..2744
                     /gene="lysS"
                     /locus_tag="VFX38_03490"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX5313957.1"
                     /translation="MTDRNERHDLVAERRRKLDVLRAEGFAYPNDFHPDTTIASLLGA
                     FGELSEAALAERPLWVRIGGRMMTRRIMGGASFAHLDDGSGRLQIFAQGESLGAEAYE
                     RFKSLDLGDVVGVEGELFRTRSGELTLRASALRLLAKCIRPLPEKFHGLKDPELRYRR
                     RYLDLAMNPDVREIFRIRAEITRVTRALLDAWGFLEVETPMMQPLAGGALARPFVTHH
                     NALDIPLYLRIAPELYLKRLLVGGLARVYEINRNFRNEGVSTRHNPEFTMLEAYESWG
                     DARSMMALAENLLKTLAADIVASPRLVYGEETIDFAADFARLTLAEATLAACPELSPE
                     DTRNAARLAEVLRARGVEAPAEAGWGKLLVALFEATAEAKLIQPTFITDYPREVSPLS
                     RAKDGDPELVDRFELFIAGREIANGFSELNDPEEQARRFEEQARERAGGDAEAMGFDR
                     DYVRALEYGMPPAGGIGIGMDRVAMLFTGAQSIREVLLFPLLRPEPDSDDAPV"
     gene            complement(2852..3868)
                     /locus_tag="VFX38_03495"
     CDS             complement(2852..3868)
                     /locus_tag="VFX38_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018874544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HEX5313958.1"
                     /translation="MSGRKILVTGAAGFIGHAVASRLLARGDAVIGLDNLNAYYDVRL
                     KEARLARLTANANFRDVRADVADRAAMTTVFARERPDGVVHLAAQAGVRYSLENPQAY
                     VESNLAGFVNLCECCRAHPVRHLVYASTSSVYGANEKLPFAVADGADHPVSLYAATKR
                     ANELIAHAYSHLFAIPATGLRFFTVYGPWGRPDMALFKFTRSMLAGEPIEVYNHGKHR
                     RDFTYIDDIAEGVVRVLDRPPAPNPNYDYGAPTPAASGAPWRVYNIGHGEPVPLMAYI
                     RALEETLGIEAKIEYLPLQPGDVEATAADVSALERDFGYRPAVAVREGIERFVAWYRE
                     YYAA"
     gene            complement(3868..>4824)
                     /locus_tag="VFX38_03500"
     CDS             complement(3868..>4824)
                     /locus_tag="VFX38_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX5313959.1"
                     /translation="RALAPGAIVVYESTVYPGATEEVCVPILEAASGLTCNRDFFVGY
                     SPERINPGDREHRLTDIVKVTSASTPEAAELVDALYRRIVRAGTHRASSIRVAEAAKV
                     IENTQRDVNIALVNELAMLFEKLGLDTHEVLAAAGTKWNFAPFRPGLVGGHCIGVDPY
                     YLTYKAISVGHHPEMILAGRRINDGMGSYVAEQIMKLLVFRRKHVADARILVLGLTFK
                     ENCPDLRNSHVIDVVRGLEGYHASVAVHDPWVDPASAREEYGIELIAEPPRGAYDAIV
                     LAVAHRDFVALGAAGIRAFGKPEALLYDVKGVLARTEVDGRL"
BASE COUNT          823 a         1541 c         1638 g          822 t
ORIGIN      
        1 gaatttgccc cgacgcgaga gaaaattgcc gatctcgaga gccgcagcga ggctctgagg
       61 aggtatcttt gactacgacg gcagtcgcga gcggctggcg gaagtagagc gcgcgctcga
      121 agcgccggag gtctggtccg atccggcgca ggcgcaacat ctcgggcgcg aacgggtgcg
      181 tctcgccggc atcgtcacga cgcttgacga ggtggcgaac tcgcttcgcg actccgcgga
      241 tctcctcgaa ctcgccgccg aagaggacga cgcgcaggcg ctcgcgcaga tcgactccga
      301 cgtcgcgacg agcgcggcgc gcatcgagga actcgaattc cggcgcatgt tcgccggcga
      361 gatggacgcg gcgaacgcct ttctcgacat ccagtcgggc tccggcggca ccgaggccca
      421 ggattggtcg gagatgctgc tcaggatgta tctgcgctgg ggagaacggc gcggtttcgc
      481 gaccgaactc atcgaggtgt ctcccggcga agtcgcgggg atcaagagcg cgaccatccg
      541 tttcggcggc gactacgcct acggctggct gcgcaccgaa accggcgtgc atcgtctcgt
      601 gcgcaaatct cccttcgatt cgggccatcg ccgccacact tccttcgccg ccgtttcggt
      661 gtcgccggag gtggacgacg aaatcgagat cgagatcaat cccgcggagc ttcgcgtgga
      721 cgtgtatcgc gcgagcggcg ccggcggcca gcacgtgaac cgcaccgaat cggccgtgcg
      781 catcacccat ctgccgacgg gcatcgtcgt tcagtgtcag aatgagcgct cgcagcacaa
      841 gaaccgggcg accgcgatga agcagttgaa ggccaagctc tacgaactcg aactctccaa
      901 gcggcgcagc gcgcagcagg aaatcgagga ggccaagagc gacatcggct ggggcagcca
      961 gatccgctcc tacgtgctcg atcagtcgcg gatcaaggat ctgcgtaccg gggtcgagat
     1021 tgccaacacc cagggcgtgc tcgacggcgc gctcgatccc ttcatcgaag ccgcgctcaa
     1081 ggcgggagtc tagccgtgtt tcggcgaaac gaaggagcgc acccgcgcgc aaactgcgga
     1141 aattctgccg cttcgcccgt gaatcgtctc atcttccccc ttcgcttgcg aagggggacc
     1201 gagggggatg tcctcgacca tgaggttttg acatgaccga ccgaaatgaa cgccacgatc
     1261 tcgtcgccga acggcgccgg aaactcgacg tgctgcgcgc ggaaggtttc gcctatccga
     1321 acgacttcca tccggacacg acgatcgcat cgctcctcgg cgccttcggc gagctgagcg
     1381 aggccgcgct cgccgagcgc ccgctctggg ttcgcatcgg cgggcgcatg atgacgcggc
     1441 ggatcatggg cggggcgagc ttcgcgcatc tcgacgatgg cagcggccgg ttgcagatct
     1501 tcgcgcaggg agaatcgctg ggcgcggaag cctacgaacg cttcaagtcg ctcgatctcg
     1561 gcgacgtcgt cggcgtcgaa ggcgaactct ttcgcacccg cagcggagaa ctcacgctgc
     1621 gtgcatcggc cctgcggctg ctcgcgaaat gcatccgccc cctgcccgag aagtttcacg
     1681 gcctgaagga tccggagctg cgctatcgcc ggcgttatct cgatctcgcg atgaatcccg
     1741 acgtgcgcga aatcttccgc atccgcgccg aaatcactcg cgttacccgc gcgctcctcg
     1801 acgcctgggg cttcctcgaa gtcgaaacgc cgatgatgca gccgctcgcg gggggcgcgc
     1861 tggcgcggcc cttcgtcacc catcacaatg cgctcgacat tccgctgtat ctgcgcatcg
     1921 cgccggaact ctatctcaag cgtctgctcg tcggcggcct cgcgcgcgtg tacgagatca
     1981 accgcaactt ccgcaacgaa ggcgtttcca cgcgccacaa tcccgagttc acgatgctcg
     2041 aggcctacga gtcctggggc gatgcgcgca gcatgatggc gcttgccgag aatctgttga
     2101 agacgctcgc ggccgacatc gtcgcctcgc cgcggctcgt ctatggtgag gagacgatcg
     2161 atttcgcggc cgacttcgcg cgcctgacgc tcgccgaggc cacgctcgcc gcctgcccgg
     2221 aattgtctcc cgaggacacg cgcaacgcag cgcgactcgc cgaagttctt cgagcacgcg
     2281 gcgtggaggc tcccgcggag gccggctggg gcaaattgct ggtcgcgctg ttcgaagcga
     2341 cggccgaagc gaagttgatc cagccgacct tcattaccga ctatccgcgc gaagtctcgc
     2401 cgctgtcgcg cgcgaaggac ggcgatcccg aattggtgga tcgcttcgag ctgttcatcg
     2461 ccgggcgcga gatcgcgaac ggcttctcgg aactcaacga tccggaggag caggcgcggc
     2521 gcttcgagga gcaggcgcgg gagcgcgccg gcggcgacgc cgaggcgatg ggtttcgatc
     2581 gcgactacgt gcgcgcgctc gaatacggca tgccgcccgc gggcggaatc ggcatcggca
     2641 tggatcgtgt cgcgatgctg ttcaccggag cccaatccat ccgcgaagta ctgcttttcc
     2701 cgctccttcg cccggaaccg gattcagacg acgctccggt ctgaggaccg gccgatcgga
     2761 aattccgcgc cgcggcgcga cgatcgcggt acgcgccgca tcgctcgatt tccgtgaacc
     2821 gcgattttgt tgacggcgaa gtcgccgccg ctcaggcggc gtagtactct cggtaccagg
     2881 cgacgaagcg ctcgattcct tcccgcacgg cgacggcggg acgatagccg aagtcgcgct
     2941 cgagcgcgct cacgtccgca gccgttgctt cgacgtcgcc cggttgcagc ggcagatatt
     3001 cgatcttcgc ctcgattccc agcgtttcct cgagagcgcg gatgtaggcc atcagcggca
     3061 cgggttcgcc gtggccgatg ttgtagacgc gccagggcgc gccgctcgcc gccggcgtcg
     3121 gtgcgccgta gtcgtaattc ggattgggtg ccggcgggcg gtcgagcacg cgcaccacgc
     3181 cttcggcgat gtcgtcgatg taggtgaagt cgcgccggtg tttgccgtgg ttgtagacct
     3241 cgatcggctc gcccgcgagc atgctgcgcg tgaacttgaa gagcgccatg tccggccgcc
     3301 cccaggggcc gtacacggtg aagaaacgca gtcccgtcgc cggaatcgca aacagatggc
     3361 tgtaggcgtg ggcgatgagt tcgttcgcgc gcttggttgc cgcgtagaga ctcaccggat
     3421 gatcggcgcc gtcggcgacc gcgaagggga gtttctcgtt cgcgccgtac accgaactcg
     3481 tggaggcgta cacgagatgg cgaacgggat gcgcgcggca gcattcgcag agattgacga
     3541 aaccggcaag gttgctctcg acgtacgcct gcgggttttc gagcgagtag cgtacgcccg
     3601 cctgcgcggc gagatggacg acgccgtccg gacgttcgcg agcgaagacg gtggtcatcg
     3661 cggcgcgatc ggcaacgtcg gcgcgaacgt cgcggaagtt cgcgttcgcc gtcaggcgcg
     3721 cgagccgcgc ctccttgagg cgcacgtcgt agtaggcgtt gagattgtct aggccgatga
     3781 ccgcatcgcc gcgcgcgagc aggcgcgagg cgacggcgtg gccgatgaat cccgcggcgc
     3841 cggtgacgag aattttccgc ccgctcatta cagccgccca tccacttcgg ttcgggcaag
     3901 tactcccttg acgtcgtaga gcagcgcttc gggcttgccg aaggcgcgaa tgccggcggc
     3961 gccgagcgcg acgaagtcgc gatgggcgac cgcgagcacg atggcgtcgt aggcgccgcg
     4021 cggcggctcg gcgatcaatt cgatgccgta ttcctcgcgc gccgaggcgg gatcgaccca
     4081 gggatcgtgc acggcgacgc ttgcgtgata gccttcgagt ccgcgcacga cgtcgatgac
     4141 gtggctgttg cgcagatcgg ggcaattttc cttgaacgtg agcccgagca ccagaatgcg
     4201 cgcatcggcg acgtgcttgc gccggaagac gaggagtttc atgatctgtt cggcgacgta
     4261 actccccatc ccgtcgttga tgcgccgccc tgcgagaatc atttccggat ggtgaccgac
     4321 gctgatcgcc ttgtaggtga ggtaatacgg gtccacgccg atgcaatggc cgccgacgag
     4381 cccggggcgg aacggcgcga agttccactt ggtgccggcg gcggcgagca cttcgtgggt
     4441 atcgagcccg agcttctcga agagcatggc gagttcgttg acgagtgcga tgttgacgtc
     4501 gcgctgcgtg ttctcgatga ccttggcggc ctcggcgaca cggatgctgg aagcgcgatg
     4561 cgttccggct cgcacgatcc ggcgatacag cgcatccacc agttcggcgg cctcgggcgt
     4621 cgaagcggag gtgaccttga cgatgtccgt gagccgatgt tcgcggtcgc cggggttgat
     4681 gcgctcggga ctgtagccga cgaaaaagtc gcgattgcac gtgagcccgg acgcggcttc
     4741 gagaatcggg acgcagacct cttcggtcgc gcccggatag acggtggatt cgtagacgac
     4801 gatcgcgccg ggggcgagcg cgcg
//
LOCUS       DASUJG010000167         2793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_172890, whole genome shotgun sequence.
ACCESSION   DASUJG010000167 DASUJG010000000
VERSION     DASUJG010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172890"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VFX38_03505"
     CDS             complement(<1..219)
                     /locus_tag="VFX38_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX5313960.1"
                     /translation="MAAVSAAEYVQPAVTADTALFSVRNDELVVLTLKRDREPFSGWW
                     ALPGDYIQSEETLDRCAARALGEQTGVAN"
     gene            complement(276..1523)
                     /locus_tag="VFX38_03510"
     CDS             complement(276..1523)
                     /locus_tag="VFX38_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018863458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5313961.1"
                     /translation="MQNRKLPLRFSDLALAPPIAAGVRDAGFEQLTPIQQRTLPDALA
                     GQDVAGQAQTGTGKTAAFLLAIFQRLLDHGASPVRRTTQPRAFIVAPTRELAIQIHRD
                     ASNLGRHTGLRMVVVYGGAGYDKQKERIAAGVDVLIGTPGRLIDFFKQHLYDLRAVEV
                     AVLDEADRMFDLGFIADIRFLLRRLPPPAERLTMLYSATLSWRVMELAHEHMRSPRFV
                     RIDPEQVTAASVSEQLYFPAMDEKPRLLVGLIRALEPERAMIFVNTRRTADRIGALLR
                     ANGIAARILSGDVPQNKRLTYLAAFESGEVPFLVTTDVASRGLHIPAVTHIFNYDLPQ
                     DAEDYVHRIGRTGRAGASGEAISFACETYAFSLPEIERFIGHEIPREFAHADFLADIE
                     RPPPGPRPSRPPARGGRGRRPRT"
     gene            1928..2254
                     /gene="trxA"
                     /locus_tag="VFX38_03515"
     CDS             1928..2254
                     /gene="trxA"
                     /locus_tag="VFX38_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009615069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEX5313962.1"
                     /translation="MSQNVSHTSDAAFETEVLKSDLPVLVDYWAEWCGPCKMIAPVLD
                     EVAGDFKSRVKVVKLNVDENAQTSRKYGIRGIPTLMLFNGGDVAAQKVGALSKSQLVA
                     FLEANL"
     gene            2401..>2793
                     /locus_tag="VFX38_03520"
     CDS             2401..>2793
                     /locus_tag="VFX38_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017845130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HEX5313963.1"
                     /translation="MNLSELKRKSATELSEVAEAVGIEGAARLRKQDMIFAILKGHAQ
                     NGEQICGDGVIEILSDGYGFLRSGDSSFQAGPDDIYVSPNQIRRFNLRTGDSVTGRIR
                     PPKPGERYFALLRVDTINDDRPEAAKSKI"
BASE COUNT          514 a          869 c          947 g          463 t
ORIGIN      
        1 gtttgcgacg ccggtctgct cgccgagcgc gcgcgcggcg cagcgatcga gcgtttcttc
       61 cgactggatg taatcgcccg gcagcgccca ccagccggag aagggttcgc ggtcacgctt
      121 gagcgtcaga acgacgagtt cgtcgttgcg cacggagaag agcgccgtat cggcggtaac
      181 ggcgggctgg acatattcgg cggcgctgac ggcggccacg gcggaaccct aagtgtcgga
      241 attacactaa atgatagtgt tttctctaca ctaaatcaag tccgcggtct gcgcccgcgc
      301 ccgccccgcg cgggcggccg tgacgggcgc gggcccggcg gtggtcgctc gatgtcggcg
      361 agaaaatccg cgtgcgcgaa ttcgcgcggg atctcgtggc cgatgaagcg ctcgatctcg
      421 ggaagcgaga aggcgtaggt ttcgcaggcg aaggaaatcg cctcgccgct cgcgcccgcg
      481 cgcccggtgc gcccgatgcg gtgcacatag tcttcggcgt cctgcggcag atcgtagttg
      541 aagatatgcg tgaccgcggg gatatgcagg ccgcgcgagg cgacgtcggt ggtgacgaga
      601 aagggcactt cgccgctctc gaaggcggcg agataggtga ggcgcttgtt ctgcggcacg
      661 tcgcccgaga gaatccgcgc cgcgattccg tttgcgcgca agagtgcgcc gatgcgatcg
      721 gccgtgcgcc gggtgttgac gaagatcatg gcgcgctcgg gctcgagtgc gcggatgaga
      781 ccgaccagca ggcgcggctt ttcatccatc gccgggaaat agagctgctc gctcacggag
      841 gccgccgtga cctgttcggg gtcaatgcgc acgaagcggg ggctgcgcat gtgctcgtgc
      901 gcgagttcca tcacccgcca ggaaagggtc gccgaataca gcatcgtgag acgctcggcg
      961 ggtggcggca ggcgccgcag cagaaagcgg atgtcggcga tgaagccgag atcgaacatg
     1021 cggtcggcct cgtcgagaac ggcgacctcg accgcgcgca gatcgtacag atgctgcttg
     1081 aagaaatcga tgagccgccc gggcgtgcca atcagtacgt ctaccccggc ggcgatgcgc
     1141 tccttctgct tgtcgtagcc ggcgccgccg tagacgacga ccatgcgcag tcccgtgtgc
     1201 cgcccgaggt tgctcgcgtc gcggtgaatc tggatcgcaa gttcgcgcgt cggcgcgacg
     1261 atgaaggcgc gcggctgcgt cgttcgccgc acgggcgaag cgccatgatc cagcaggcgc
     1321 tgaaagatcg cgagaagaaa ggccgcggtc ttgcccgtgc cggtctgcgc ctggccggcg
     1381 acatcctgcc cggcaagcgc atcgggaagc gtacgttgtt gaatcggcgt caattgctcg
     1441 aagccggcgt cgcgcacgcc ggccgcgatc ggcggcgcga gcgcgaggtc ggagaagcgc
     1501 aaggggagtt ttcggttctg cattctggga agattctcgt aggcgtgtgg cgcggggctt
     1561 gcattggcgc gcgaatggcc ccacaatagg cagcggatgg gatcagggcg gccccgcggc
     1621 cggcctgagt gatcgggccg tttcgagcca accagactca caccagcagt ctctattgtg
     1681 ccgcatgcgg cccgccgttc ctaacctgtc cggcccagca ccctgctcat ggatctcaat
     1741 cgtgccgccg gacgcgcatt tgcatcgccg cttcgcgcgc actggcgcac gatccggttg
     1801 gaaggcaaat ccgggctctg ccgggtaagc cgtctccgat ccgttccgtc gcgaatgcat
     1861 cggagcttcc gttctttccc tggcggcgaa aagccgtcca tggttgtcgt cgaactggag
     1921 gatcaaggtg agtcagaatg tttcccatac gtcggatgca gccttcgaga ccgaagtgct
     1981 caagtccgat ctgcccgtgc tcgtggatta ctgggccgag tggtgcggtc cctgcaagat
     2041 gatcgcaccg gtgctcgatg aagtcgcggg cgacttcaag tcgcgcgtca aggtcgtcaa
     2101 actgaacgtc gacgagaatg cgcagacctc gcgcaaatac ggcattcggg gcatccccac
     2161 gctgatgctc ttcaatggcg gcgacgtcgc cgcgcagaag gtgggagcgc tgtcgaaatc
     2221 ccagctcgtc gcgttcctgg aggcaaacct ttgagcgggc atgcataccg gcgccgcaac
     2281 ccccggcgca acggcaacaa tcgtcgcgaa tccgacggca acgtcctcgc ccccgggatt
     2341 gaaatggaag acacggacga atcgcaatac gaaaacgacg aggccggcaa cggccccacg
     2401 ctgaacctct ccgaactcaa acgcaagagc gccacggaac tttccgaagt tgcggaagcc
     2461 gtggggatcg aaggtgccgc gcgcctgcgc aagcaggaca tgatcttcgc gatcctcaag
     2521 ggccacgccc agaacgggga gcagatctgc ggcgacggcg tgatcgaaat cctttccgac
     2581 ggctacgggt tcctgcgatc cggagacagc tcctttcagg cggggcccga cgacatctac
     2641 gtgagcccga accagatccg gcgcttcaac ctgcgcaccg gcgattcggt caccggaaga
     2701 atccgcccgc cgaagccggg cgagcgctac ttcgcgctgc tccgtgtgga caccatcaac
     2761 gacgaccgcc cggaggcggc gaaatcgaag att
//
LOCUS       DASUJG010000168         3051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_173242, whole genome shotgun sequence.
ACCESSION   DASUJG010000168 DASUJG010000000
VERSION     DASUJG010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173242"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VFX38_03525"
     CDS             complement(<1..369)
                     /locus_tag="VFX38_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006912681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5313964.1"
                     /translation="MAKMDELKRYRDKRDFSRSPEPSGRRRRSGRKPAIFVIQKHDAS
                     HLHYDFRLQIGRVLRSWAVPKGPSTDPRVKRLAVETEDHPFDYADFEGIIPAEEYGGG
                     TVLVWDAGPYRDLRNDRGSSP"
     gene            405..1358
                     /locus_tag="VFX38_03530"
     CDS             405..1358
                     /locus_tag="VFX38_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015005245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX5313965.1"
                     /translation="MARSTTMESSSTWVVIAALAGNAAVAVIKFFAAFLSGSAALMSE
                     GIHSLVDTADQGLLLYGLRRARKPADEAFPFGYGKEVYFWSFVVATQLFSIGALASIY
                     EGVLHILHQSAIEDATLGYIVLGLAFVFEASSWSFAVFNFSRSKGRRSYVEAIRVAKD
                     PTVFVVLFEDSAALLGIVIAFLGIFLSRKTGIPWFDGVASILIGVILGVTAILLAREV
                     KGLLIGESANRPVVKGIRELAASFPEIRAVNEALTMHVGPNFILVNLSVAFAEQLNAG
                     EVASAAAHLDVAIKERYPRVKRVFIEAKTAFLARSVRTRTE"
     gene            1574..2128
                     /locus_tag="VFX38_03535"
     CDS             1574..2128
                     /locus_tag="VFX38_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015956418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEX5313966.1"
                     /translation="MASGLHGKKVAILATDGFEQSELLEPRRALKEAGAATKVVSPAD
                     GKIRGWNHGDWGEKVDVDVPLDNAQAADYDALLLPGGVMNPDRLRMKRRAVEFVREFF
                     EGGKPVAAICHGPWMLVEAGVVEGRTLTSWPSLATDIRNAGGTRVDEEVFVCTGGPNT
                     LVTSRKPDDIPAFNEKMLEAFAQA"
     gene            2221..>3051
                     /locus_tag="VFX38_03540"
     CDS             2221..>3051
                     /locus_tag="VFX38_03540"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5313967.1"
                     /translation="MSQEQTSAQSAGGGAGAVPADESRQPWPRTLAVLGVMAGCFAFQ
                     EMAILPALPMIQRQLAGANTTTTALLESGFLIVAAVAAPLLGKLGDCRGKKNMLLATL
                     VVYFAGSLGAGLAPNFIALILFRALQGVGGALMLLSIAISRDELPAEKLGIGIGWIVG
                     SFGAGACVGLGLSGVITEFLSWRFIFFVECALIAIGGILVLELLPDSRDRCAKPVDYA
                     GVALLGCALASLIMGLTETLSLGWPVVGLFLLAAVLFTAWIFHEKRTDAPLLDVAVLA
                     S"
BASE COUNT          511 a          939 c         1000 g          601 t
ORIGIN      
        1 cgggctgctc ccacgatcgt tgcgcagatc gcggtagggg ccggcgtccc atacgagaac
       61 ggtgccgccg ccatattcct ccgccggaat gatgccctcg aagtcggcat agtcgaaggg
      121 atgatcttcg gtctcgaccg cgagtcgttt cacgcgcgga tcggtggacg ggcccttcgg
      181 aaccgcccag gacctgagca cccggccaat ctgcaggcgg aaatcgtagt gaagatgcga
      241 ggcgtcgtgc ttctggatga cgaagatcgc gggcttgcgg ccgctgcgcc gccgtcttcc
      301 ggatggctcc ggagagcgcg agaagtcccg cttgtcgcga tagcgcttga gttcgtccat
      361 tttggccatg ttcggatgct agcataggaa atccgccgtt ttttgtggcc cgcagcacca
      421 cgatggaaag ttcctcgaca tgggtagtga tcgccgcgct tgcaggcaat gccgcggtcg
      481 cggtgatcaa gttctttgcg gcgttcctgt cgggcagcgc cgccctgatg tccgaaggga
      541 ttcattcgct cgtggacacg gccgatcagg gccttctcct ctacggattg cggcgcgcgc
      601 gaaagcccgc cgacgaggcc tttccgttcg gctacggcaa ggaagtgtac ttctggagct
      661 tcgtggtcgc cacgcagttg ttttcgatcg gcgctctcgc ctcgatctac gagggagtcc
      721 tgcacatcct gcaccagagc gcgatcgagg atgcgacact cggttacatc gtgctcggcc
      781 tcgcttttgt cttcgaagca agcagctgga gcttcgcggt cttcaacttc tcccggagca
      841 agggccggcg ttcctatgtc gaggcaatcc gggtcgccaa ggacccgacc gtcttcgtcg
      901 tgctcttcga ggactccgcg gccctgctcg gaatcgtgat cgcttttctc gggatttttc
      961 tcagccggaa aacgggaatt ccctggttcg acggcgtcgc ctcgattctg atcggcgtca
     1021 tcctcggcgt gaccgcaatt ctcctcgctc gcgaggtcaa gggcctgctg atcggggaga
     1081 gcgccaatcg cccggtcgtc aagggcatcc gcgaactcgc cgcgtcgttc cccgaaatcc
     1141 gcgccgtgaa cgaagctctc acgatgcacg tgggaccgaa cttcatcctg gtgaacctca
     1201 gcgtcgcatt tgcggaacaa ctcaacgccg gcgaggtggc gtctgcggcg gcgcacctcg
     1261 acgtggcgat caaggagcgg tacccgcgcg tcaagcgcgt attcatcgag gcgaaaaccg
     1321 cctttctcgc ccgcagcgtg cgcacccgca cggaataacg tcaggccctc gttgcatagt
     1381 ggagtcgggc gtgcgttatg gatgaccgcg catcgcggat tgcttcccgt gtatggaagg
     1441 cttccgggac tctacgacga aggcggcgtc gcgaggcggg gcgcggcgga agcggtttcg
     1501 catcggcgcc gcggttggcg ccgcctcgcc gacaaaatgg ggggaatgac gggtcgaacc
     1561 cgaggagacg atcatggcat cgggattgca tgggaaaaag gtcgcgattc tcgcgaccga
     1621 cggctttgag caatcggagc ttctggaacc gcgccgcgcg ctcaaggagg cgggagccgc
     1681 aacgaaagtg gtctcgcccg ctgacggcaa gatcaggggc tggaatcacg gtgactgggg
     1741 cgagaaagtg gacgtggacg tgccgctcga caacgcacag gcagcggact acgatgcgct
     1801 gcttcttccc ggcggcgtga tgaatcccga tcggcttcgc atgaagagac gtgccgtcga
     1861 attcgtgcgc gagttcttcg agggaggaaa accggtagca gcgatctgcc acggcccatg
     1921 gatgctggtc gaggccggag tcgtcgaggg gcgcaccctg acctcctggc cgagcctcgc
     1981 gaccgatatc cgcaatgccg gcggcacgcg cgtggacgaa gaagttttcg tgtgcaccgg
     2041 cggtccgaat acgctcgtca ccagccgcaa gccggacgat attcccgcct tcaacgaaaa
     2101 gatgctcgaa gccttcgcgc aagcgtaacg caaaaaaaag cagccaccgg cggtgattcg
     2161 ccaatcgaga cgatgccgcg gcaaggcggc gtcgtttttc ggcaccgagg cggatcgctg
     2221 atgtcgcagg agcaaacatc cgcgcagtca gccggcggcg gcgcgggcgc cgtgccggcc
     2281 gatgagtctc ggcagccgtg gccgcgcacc ctcgcggttc tcggtgtcat ggccggctgc
     2341 ttcgcctttc aggaaatggc gatcctgccg gcgctgccga tgatccagcg gcaactggcg
     2401 ggagcgaaca ccaccactac cgcgctgctg gaaagcggat tcctcatcgt ggcggcggtt
     2461 gcggcgccgc ttcttggcaa gctcggtgac tgtcgcggca agaagaacat gctgctcgcg
     2521 accctcgtcg tttacttcgc cggttccctc ggcgccgggc tcgcacccaa tttcattgcc
     2581 ttgattctat ttcgcgcact gcaaggcgtc ggcggagccc tgatgcttct gagcatcgcg
     2641 atttcgcgtg acgaactccc cgccgaaaaa ctcggcatcg ggatcggatg gatcgtcggc
     2701 tcctttgggg ccggcgcctg cgtcgggctt ggtttgtcgg gcgtcatcac cgaattcctg
     2761 tcttggcgct tcatcttttt cgtcgaatgc gcgctgattg cgatcggcgg cattctggta
     2821 ctggagttgt tgccggactc gcgggatcgc tgcgcgaagc cggtggacta cgccggcgtc
     2881 gccttgctcg gctgcgcgct cgccagcctg atcatggggc ttacggaaac gttgtcactg
     2941 ggctggccgg tcgttggact ctttcttctc gcggccgtgc tttttacggc ctggatcttc
     3001 cacgagaagc ggacggacgc tccgctgctg gacgtcgccg tgctggcgag c
//
LOCUS       DASUJG010000169         2337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1272883, whole genome shotgun sequence.
ACCESSION   DASUJG010000169 DASUJG010000000
VERSION     DASUJG010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272883"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /gene="sucD"
                     /locus_tag="VFX38_03545"
     CDS             complement(<1..864)
                     /gene="sucD"
                     /locus_tag="VFX38_03545"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX5313968.1"
                     /translation="MSILIDKTTKVLCQGFTGRQGTYHSETAIEYGTRLVGGVTPGRG
                     GETHLDLPVFDTCAEAVAATGATASVIYVPAPFAADAILEAADAGIELVVCITEGIPV
                     LDMVRVKAALAGSALRLVGPNCPGVITPGECKIGIMPGYIHQPGRVGIVSRSGTLTYV
                     AVHQTTLEGLGQSSCVGIGGDPVHGMNFIDVIALFEKDPATEAIVMVGEIGGGDEEAA
                     AEYIHAQVTKPVVAYIAGVTAPPGKRMGHAGAIVSGGKGTAEAKFAALEAAGVATVRS
                     PAEIGKAVKSAL"
     gene            complement(861..2036)
                     /gene="sucC"
                     /locus_tag="VFX38_03550"
     CDS             complement(861..2036)
                     /gene="sucC"
                     /locus_tag="VFX38_03550"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017840697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX5313969.1"
                     /translation="MNLHEYQAKGLFAAFDIPVPEGRVAASAAEAEAAARTLGGALWV
                     VKAQVHAGGRGKAGGVKLCRSPEEAGAAAEAMLGTRLATHQTAGAGLPIERVWVERGS
                     KIARELYLSLLVDRGRERFVFIASAAGGMEIEEVAAKTPEKIATVAVSPAAGFSGFEA
                     RRLAFALGLDAAQRRPFAKLARSLYALALARDASLVEINPLIVTEDGALVALDAKLNI
                     DDNALFRQEELAAMRDPSQEDPAEAEAGEHGLNYVALDGDIACMVNGAGLAMATMDLI
                     QLAGGEPANFLDVGGGATTERVSAAFRLILANPKVRAILVNIFGGIVRCDIIAEGIIA
                     AVKGVGVAVPVVVRLEGTKAEEGRRLLAESGFGISAAENLADAADKVVAAVRDGERR"
     gene            2082..>2337
                     /locus_tag="VFX38_03555"
     CDS             2082..>2337
                     /locus_tag="VFX38_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313970.1"
                     /translation="MNEIAKDRLFWAIGRRMAKRGAAPASPRIEQALRWRLLELLNIY
                     RLLLATAIFLVGLVPAFSRALALEAPRLTMLAGLVYLLFAL"
BASE COUNT          345 a          846 c          754 g          392 t
ORIGIN      
        1 gagcgcggac ttcacggcct tgccgatttc cgcgggcgag cgcacggtcg cgacccccgc
       61 ggcttcgagc gcggcgaact tcgcctccgc cgtgcccttg ccgccggaga cgatggcgcc
      121 ggcgtggccc atgcgcttgc cgggtggcgc ggtaacgccg gcgatgtagg cgacgacggg
      181 cttggtcacc tgcgcgtgga tgtattcggc cgccgcctcc tcgtcgcccc cgccgatctc
      241 gccgaccatg acgatcgcct cggtcgccgg atccttctcg aagagcgcga tcacgtcgat
      301 gaaattcatg ccgtgcaccg gatcaccgcc gatgccgaca cagctcgact ggccgagacc
      361 ttcgagcgtg gtctgatgca cggcgacgta ggtgagcgtg cccgagcgcg agacgatgcc
      421 gacccggccg ggctggtgaa tgtagccggg catgatcccg atcttgcact cgcccggcgt
      481 gatgactccc ggacaattcg ggccgacgag ccgcagcgcg cttcccgcga gcgccgcctt
      541 cacgcgcacc atgtcgagca ccggaatgcc ctcggtgatg cacacgacca gttcgattcc
      601 cgcgtccgcg gcctcgagaa tcgcatccgc ggcgaagggc gcgggtacgt agatcacgct
      661 cgcggtggcg ccggtcgccg cgaccgcctc cgcgcaggtg tcgaagaccg gaaggtcgag
      721 atgggtttcg ccgccccgtc ccggcgtcac tccgccgacg aggcgggtgc cgtattcgat
      781 cgccgtttcg ctgtgatagg ttccctgccg cccggtgaat ccctggcaga gtactttcgt
      841 cgtcttgtcg atcagaatgc tcatcggcgt tcgccatcgc ggaccgcggc gacgaccttg
      901 tccgcggcat cggcgaggtt ctcggcggcg ctgatgccga atccgctttc ggcgagaagc
      961 cgccggcctt cctcggcctt cgtgccttcg aggcgcacga cgaccggaac ggcgacgccg
     1021 acgcccttca ccgcggcgat gatgccctcg gcgatgatgt cgcagcgcac gatgccgccg
     1081 aagatgttga cgagaatcgc gcgcaccttc gggttcgcga gaatcaggcg aaaggccgcg
     1141 ctcacccgct cggtcgtcgc cccgccgccc acgtcgagaa agttcgcggg ctcgccgccc
     1201 gcgagctgaa tgagatccat cgtcgccatc gcgagtccgg cgccgttcac catgcaggcg
     1261 atgtcgccgt cgagggcgac gtagttgagt ccgtgttccc cggcctccgc ctcggccgga
     1321 tcctcctgac tcggatcgcg catcgccgca agctcctctt gccggaagag cgcgttgtcg
     1381 tcgatgttga gcttggcatc gagcgcgacc agcgcgccgt cctcggtgac gatgagcgga
     1441 ttgatttcga cgaggcttgc atcgcgcgcg agcgcaagcg catagagcga gcgcgcgagt
     1501 ttcgcgaagg gtctgcgctg cgcggcgtcg agcccgagcg caaaggcgag ccgtcgcgcc
     1561 tcgaagccgg aaaatcccgc ggccgggctc acggcgacgg tcgcgatctt ctccggcgtc
     1621 ttcgccgcga cttcctcgat ctccatgccg ccggcagccg aggcgatgaa cacgaagcgc
     1681 tcgcgcccgc gatcgacgag gaggctcaga tagagttcgc gcgcaatctt cgagccgcgc
     1741 tcgacccaga cccgctcgat cggcagtccc gccccggcgg tctgatgcgt cgcgagtctc
     1801 gttccgagca tcgcctccgc cgcggccccc gcctcctcgg ggctgcgaca gagtttcacc
     1861 ccgcccgcct tgccgcggcc gcccgcatgt acctgcgcct tgaccaccca cagcgcaccg
     1921 cccaaagtgc gcgccgcggc ctcggcctcg gccgcgctcg ccgcgacccg cccctcggga
     1981 acgggaatat cgaaggcggc aaacaggccc ttcgcctgat attcgtgcag attcatggta
     2041 tagaaagcct ggttttccgt tcccgcggcg gcgcgaaacg catgaacgag atcgcgaaag
     2101 accgcctatt ctgggccatc gggcgccgaa tggcaaaacg cggggccgcg ccggcgagcc
     2161 cacgcatcga acaggccctg cgctggcgcc tgctcgaatt gctcaatatt taccgcctgc
     2221 tgctcgccac cgcgattttt ctcgtcggcc tcgtgcccgc attttcgcga gccctcgcgc
     2281 tcgaggcgcc gcggctcacg atgcttgccg gcctcgtcta tctgctcttc gccctcg
//
LOCUS       DASUJG010000170         2153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_662658, whole genome shotgun sequence.
ACCESSION   DASUJG010000170 DASUJG010000000
VERSION     DASUJG010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662658"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..678)
                     /locus_tag="VFX38_03560"
     CDS             complement(67..678)
                     /locus_tag="VFX38_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX5313971.1"
                     /translation="MDLTQQVLRTDAAGLPLEWIGYQEAVRLYALEQIAYTLGQRLFD
                     LRGGINAASGLRSRIEIHSIVATYGKSVAASRMTSGYVPPVSNAALFKRDAYLCLYCG
                     QHLAARYLSRDHVTPFSRGGSDDWRNLATACRRCNNHKAGRRPEEADMQLIAIPFTPS
                     YAEYIYLKGRRILADQMEFLKAHFPRTSPLLARWSALPPAGTA"
     gene            903..1757
                     /locus_tag="VFX38_03565"
     CDS             903..1757
                     /locus_tag="VFX38_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008959986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX5313972.1"
                     /translation="MNDPLAFLLPYEFSPIVLAACLAAAAIYARGLLRRRRRGLADGW
                     IRPLGYFLGVALMYGVLQTHYDYMSQHMFFLHRLQHLVLHHLAPFLIMLSVPGPILVA
                     GVPGPVRRRLLRPLARSPAVRIPYRIVQQPIVAATLFFGLIYLWLFPSLHVYAMLNVP
                     LYDTMNWSMAIDGLFFWYLMLASPGPMRGGLHYGWRMLILGAVIPPQIVVGAYIALSG
                     KDLYTIYAVCGRLWPINAMTDQVIGGLVTWIPAAMMSAIGAIVLLHRWMHEENAALEP
                     AQPKSAYA"
     gene            1750..>2153
                     /locus_tag="VFX38_03570"
     CDS             1750..>2153
                     /locus_tag="VFX38_03570"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEX5313973.1"
                     /translation="MRNSLAALGFAFCTCLLPMPAFAAAPPITVHDAWIRLLPADLPL
                     AGYFTLHNAAPQTIELLAVTSPAFTRIELHRSSESGGMEHMAAVARIAIPSGHTLRFA
                     PGGYHLMLWRARALHVGEKVPLTLEFTDGRKIT"
BASE COUNT          340 a          752 c          650 g          411 t
ORIGIN      
        1 gaaccagagc ccgcaggttt ttgctcgcgc tcgtgccggc tttcgagcgg aacatcgcga
       61 cggctttcag gcggttcctg cgggcggcag ggcggaccag cgtgcgagca gcgggctcgt
      121 gcgcgggaag tgcgccttca gaaactccat ctggtcggcg agaatgcgcc ggcccttgag
      181 atagatgtat tcggcatagc tcggcgtgaa gggaatcgcg atgagttgca tgtcggcttc
      241 ttcgggtcgc cgtcccgcct tgtgattgtt gcagcggcga caggccgtgg cgagattgcg
      301 ccagtcgtcg ctgccgccgc ggctgaacgg agtcacgtga tcgcgcgaca gataccgcgc
      361 cgcgagatgc tgcccgcagt aaagacaaag gtaggcatca cgcttgaaga gcgcggcgtt
      421 ggagaccggc ggaacgtaac cggaggtcat gcggctcgcg gccacgctct tgccgtaggt
      481 cgcgacgatc gagtgaatct cgatgcgcga acgcaggccg ctcgcggcgt tgatgccgcc
      541 gcgaagatcg aagagccgct ggccgagcgt gtaggcgatc tgctcgaggg catacagacg
      601 cacggcctcc tggtagccga tccactccag cggcagtccc gccgcatccg ttcgcaagac
      661 ctgttgggtc agatccatgc ccttgcccgc gctccgatcg cgctcaagca tgccgcagca
      721 ttcggcgcaa tgtcaaggag aacgcgcacg gcgccggcgc gcgcgcccgc aatcgcgtcc
      781 cgaacaacgc agccggccgc gcaaaccgca gcgccgcggg cctctactcg ggactcgcgg
      841 caaggcttgg actccggcgc tcgggcaatc atgcaaggta ttttttcggc agtttctgcg
      901 gcatgaacga tcccctcgcc tttctgctgc cctacgagtt ttccccgatc gtgctcgccg
      961 cctgcctcgc cgcggcggcc atctacgcgc ggggactgct gcgccggcgc cgccgggggc
     1021 tcgccgacgg ctggatccgc ccgctcggat attttctcgg agtcgcactg atgtacggcg
     1081 tactccagac ccactacgac tacatgtccc agcacatgtt cttcctgcat cgcctgcaac
     1141 atctcgtgct gcatcacctc gcgcccttcc tcatcatgct gtcggtgccg ggtcccattc
     1201 tcgtcgccgg cgttcccggc cctgtgcgcc gaaggttgtt gcgcccgctc gcgcgctcgc
     1261 ccgccgttcg cattccctat cgcatcgtgc aacagccgat cgtcgccgca accctcttct
     1321 tcggtctgat ctatctctgg ctcttcccat cattgcacgt ctacgcgatg ctcaatgtgc
     1381 cgctctacga caccatgaac tggagcatgg cgatcgacgg cctcttcttc tggtatctga
     1441 tgctcgcctc tcccggcccg atgcgcggcg gactgcacta cggctggcgc atgctcatcc
     1501 tgggcgccgt catcccgccg cagatcgtcg tcggcgccta catcgcgctc tcgggcaagg
     1561 acctatacac gatctatgcg gtttgcggac ggctgtggcc gatcaacgcg atgaccgatc
     1621 aggtcatcgg cgggctggtc acctggattc cggcggcgat gatgagtgcg atcggcgcga
     1681 tcgtgctgct gcaccgctgg atgcacgagg aaaatgccgc gctcgaaccc gcccaaccga
     1741 aatcggccta tgcgtaactc actcgccgcg ctcggcttcg cgttctgcac ctgcctgctg
     1801 cccatgccgg ctttcgcggc ggcgccgcca atcacggtgc acgacgcttg gattcgactt
     1861 cttcccgctg accttccgct cgccggctac ttcacgctgc acaatgccgc cccgcaaacg
     1921 atcgagctgc tcgccgttac gagtcccgcg ttcacccgca tcgaactcca tcgcagcagc
     1981 gaaagcggcg gcatggaaca catggccgcc gtcgctcgca tcgcaattcc ctccggccac
     2041 acgctccggt tcgcaccggg cggctaccat ctcatgcttt ggcgcgcgcg cgcgcttcat
     2101 gtcggagaga aggttccgct cacgctcgaa ttcaccgacg gccgcaagat cac
//
LOCUS       DASUJG010000171         2921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_541026, whole genome shotgun sequence.
ACCESSION   DASUJG010000171 DASUJG010000000
VERSION     DASUJG010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541026"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..2425
                     /gene="ligA"
                     /locus_tag="VFX38_03575"
     CDS             83..2425
                     /gene="ligA"
                     /locus_tag="VFX38_03575"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX5313974.1"
                     /translation="MPEEPASAERRIEMIRAQLRHHNYRYYVENDPEVSDADYDRLLR
                     ELQELEAAHPELVTPDSPSQRVGAPPDTAFAEVRHRPPMLSLANAFGDEEVREFDRRV
                     RARLDQRDPIEYMAEPKFDGLAVSLLYEAGRLVQAATRGDGETGEDVSANVRTIRAVP
                     LVLRAEGEPPNRLDVRGEVYMPRDGFKRMNAEAAKRGEKSFVNPRNAAAGSLRQLDPR
                     VSASRPLAFFAYAVGSGEEELGVGRQSELLERLERLGLPVCRERRRVRGVEGCLAFHR
                     ELGERRAKLAFDIDGVVFKVDRLEWQRALGSVSRAPRWAIAHKFPAEEATTRLRAIEW
                     NVGRTGALTPVAKLEPVFVGGVTVSNATLHNPEELARKGVWVGARVVVRRAGDVIPEV
                     VRVIGKPPAGTEFPRPPETCPVCGSRVAQRERETRGRGGRSSRTRFAVWECLGRMQCP
                     AQLARSLEHFVSRSAADIEGFGEKLCAMLVATGDVKTLSDIYRLKAETLRELEGYAEL
                     SAANLMRAIDARRRLPLARFLNAIGIPEIGEVGAKQIAQLLGSLERLRDCPAEVLACF
                     EGIGIGTGRLVEEFFRDRNSRSALDSFFTRDTGFSVEEAAPAPEAYAAVGFGRLVNNL
                     QIAGLGEKTGNDLGGRLQRFSDLADFARNGKVSGIPDKAALGLQQFLAEKENRERIRA
                     IDAWLERRGVHAANAPRRTRASAGRLAGKSFVLTGTLDGMTREEARERIEREGGKVTG
                     SVSRKTDYLVAGAQAGSKLSRAREFEVPVLDQAAFLRMIG"
     gene            complement(2454..>2921)
                     /locus_tag="VFX38_03580"
     CDS             complement(2454..>2921)
                     /locus_tag="VFX38_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313975.1"
                     /translation="GGGSDKSAVCATNTGATMRLDEAILSLVKERSISDQKELLLRLE
                     EAGVAATQPTLSRHMKKLGVRKLAGRYQRIELSGTPIPPFSLTPVPPGLLVLRTRPGY
                     AQAVAIWLDQKNLASLVGTIAGDDTVFIAVRNPPPLETVAETLSRLLRENAPG"
BASE COUNT          506 a          942 c          998 g          475 t
ORIGIN      
        1 ggccacatcg aggatcgcga acatcatccg ctcaccgccg cccgcgaact cgaactcaag
       61 ttgacggcgg ccggcgctcg cggtgcctga ggagccggct tccgccgaac gccggatcga
      121 gatgatccgc gcgcaattgc gccatcacaa ctatcgctac tacgtcgaaa acgatcccga
      181 agtttccgac gccgattacg atcgcctcct gcgcgagctg caggaactcg aggcggcgca
      241 tcccgaactc gtcactcccg acagcccgag ccagcgggtc ggcgcgcccc cggacaccgc
      301 cttcgccgag gtgcggcatc gtccgccgat gctttcgctc gccaacgcct tcggcgacga
      361 agaagtgcgc gaattcgatc ggcgcgtgcg cgcgcggctc gaccagcgcg atccgatcga
      421 atacatggcg gagccgaagt tcgacgggct cgcggtgagc ctcctctacg aagcgggaag
      481 actggtacag gccgcgaccc gcggcgacgg ggaaaccggc gaagacgtga gcgcaaacgt
      541 acgcacgatc cgcgcggtgc cgctcgtgct gcgtgccgaa ggcgaaccgc cgaaccggct
      601 cgacgtgcgc ggcgaggtgt acatgcccag ggacggtttc aagcgcatga acgccgaagc
      661 ggcaaagcgc ggcgagaaga gcttcgtcaa tccgcgcaac gccgcggccg gcagcctgcg
      721 tcagctcgac cctcgagtga gcgcctcgcg cccgctcgca tttttcgcct acgccgtcgg
      781 ctccggagaa gaagaactcg gggtggggag acagagcgaa ctcctcgaac ggctcgaacg
      841 gctcggcttg ccggtctgcc gcgagcggcg ccgggtacgc ggcgtcgaag gctgtctcgc
      901 gtttcatcgc gaattgggag aacggcgcgc gaagcttgcc ttcgacatcg acggcgtggt
      961 cttcaaggtc gatcgcctcg agtggcagcg cgcgctcggc agcgtttcgc gggcgccacg
     1021 ctgggcgatc gcgcacaagt tcccggccga ggaggcgacg acgcggctgc gcgcgatcga
     1081 atggaacgtc gggcgcacgg gcgcgctcac tccggtcgcg aaactcgaac cggtgttcgt
     1141 cggcggtgtg acagtgtcga acgcgacctt gcacaatccc gaagaactcg cgcgaaaggg
     1201 cgtctgggtc ggtgccagag tcgtcgtgcg gcgcgcgggc gacgtcattc ccgaggtcgt
     1261 gcgcgtgatc gggaagccgc cggcgggaac ggaattcccg cgccctcccg aaacctgtcc
     1321 ggtgtgcggg tcgcgcgtgg cccagcgcga gcgcgaaacg cgcgggcgcg gcggacgcag
     1381 ttcgcgcacc cggtttgcgg tctgggaatg cctgggacga atgcaatgtc cggcgcaact
     1441 cgcgcgctcg ctcgaacatt tcgtgtcgcg aagcgcggcg gacatcgagg ggttcggcga
     1501 aaaactttgc gccatgctcg tcgcgaccgg cgacgtcaag accctctccg acatctatcg
     1561 tctgaaagcc gagacgctgc gcgaactcga gggatacgcg gagctttccg cggccaatct
     1621 gatgcgggcg atcgatgcgc gccgccgcct gccgctcgcc cgtttcctca acgccatcgg
     1681 aatcccggaa atcggcgagg tcggcgcgaa gcaaatcgcg cagcttctcg gcagcctcga
     1741 gcggcttcgc gactgcccgg cggaggtcct ggcctgcttc gagggaatcg ggatcggcac
     1801 cgggcgcctc gtcgaggagt ttttccggga tcgcaacagc cgcagcgcgc tcgattcctt
     1861 cttcactcgc gacaccggct tttcggtgga ggaggcggca ccggcacccg aggcctatgc
     1921 tgccgtcggc tttggcagac tcgtcaacaa tctgcagatt gcgggtctcg gcgaaaagac
     1981 agggaacgat ctcgggggcc ggctccagcg cttttcggat ctcgccgatt tcgctcgaaa
     2041 cggaaaggta tccggcattc cggacaaggc agcgctcgga ttgcagcaat ttctggcgga
     2101 gaaggagaat cgggagcgaa tccgcgcgat tgacgcatgg ctcgaacgca ggggtgtcca
     2161 tgccgccaac gcgccgcggc gaacccgggc gagtgccggt cgtctggccg gcaagtcctt
     2221 cgtgttgact gggacgctcg acggcatgac ccgcgaggaa gcgcgggagc gaatcgagcg
     2281 cgagggcggc aaggtgacgg gcagcgtttc gcgcaaaacc gattatctgg tcgcgggcgc
     2341 gcaagccgga tcgaagctca gccgcgcccg cgaattcgaa gttccggtgc tggatcaggc
     2401 ggcctttctc aggatgatcg gctaggctcc cgtttttttt gcggagcgat gtttcaaccc
     2461 ggcgcattct cccgcagcag tcggcttagc gtttcggcaa cggtttccag cggcggcgga
     2521 ttgcgcacgg cgatgaagac cgtatcgtcg cccgcgatcg taccgacgag gctcgcgagg
     2581 tttttctgat cgagccagat cgcgacggcc tgcgcgtatc ccgggcgggt gcgcagcacg
     2641 aggagacccg gcggcaccgg cgtgagcgaa aagggaggaa tcggcgtacc ggacaattcg
     2701 atgcgctgat agcggccggc gagtttgcgc actccgagct ttttcatgtg gcgcgagagc
     2761 gtcggctgcg tcgcggcgac acccgcttct tccaggcgca gcaacaactc cttctggtcc
     2821 gagatgctgc gttccttcac caggctgaga atggcttcgt ccagacgcat ggtggccccc
     2881 gtattcgtcg cgcaaacggc gcttttgtcg cttccgccgc c
//
LOCUS       DASUJG010000172         3285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_908631, whole genome shotgun sequence.
ACCESSION   DASUJG010000172 DASUJG010000000
VERSION     DASUJG010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908631"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VFX38_03585"
     CDS             <1..279
                     /locus_tag="VFX38_03585"
                     /inference="COORDINATES: protein motif:HMM:NF023125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3293 domain-containing protein"
                     /protein_id="HEX5313976.1"
                     /translation="AWNPGTRRPSSRENREANRRLAAEIVRQGWSFWPARGRNENGLH
                     VEESFAVERIAADAAIALAHRFHQAAVFYWDGSEACILPASPAGMTAT"
     gene            293..529
                     /locus_tag="VFX38_03590"
     CDS             293..529
                     /locus_tag="VFX38_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HEX5313977.1"
                     /translation="MLEMRPNCECCGRPLAPDARAYVCSLECTFCPSCAEETLRRVCP
                     NCGGELRLHPIRAARLLARFPASAGKIVKLGGCG"
     gene            597..1115
                     /locus_tag="VFX38_03595"
     CDS             597..1115
                     /locus_tag="VFX38_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313978.1"
                     /translation="MNYRILALIGVRLLAIWLIVLGISALPDIQLTWQAAIASHHPSQ
                     AWITFGYAMVMLAIVVPLILGVCLWALGPWLARRITPAEEDAPPDATAPVLVQGAIAI
                     AGLVLAVTTLPGLIFAIVTVVREAAQPLGIREQTWTTFGYRMILILLALGLVVGARRL
                     RDWIFRLRTMGT"
     gene            complement(1260..>3285)
                     /locus_tag="VFX38_03600"
     CDS             complement(1260..>3285)
                     /locus_tag="VFX38_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5313979.1"
                     /translation="EGFYTIGSAGHEGNVLLGRLARRTDPGFLHYRSGAFFIERARQL
                     PELDPVYDMALSFAASSDDPASGGRHKVFGSAPLWLPPQTSTIASHLPKAVGAALAIA
                     PAARAKCLPIPSDSIVVCSFGDASVNHATALAGFNAAAQSAFRKLPCPILFVCEDNGI
                     GISVETPAGWVEANFSQRPGLHYVKADGLDLAQGYGAVAEAVEFCRSRRAPVFLHLRT
                     VRLLGHAGTDVEVEYRGIEKVEADEARDPLLFSAETALELGLTTPGALLARYEACRER
                     VREASRRAANRPRLRSRRDVMAALAPLDAAGVKAEAERTDFEAAREAACGGAARLPED
                     GPPRHLSIQINRALVELLAKYPEMTLFGEDVAAKGGVYTVTSGLHAKFGARRVFNTLL
                     DETTILGLAQGQAMMGLLPFPEIQYLAYLHNALDQLRGEAASLQFFSNGRFSNGMVVR
                     IAGLAYQKGFGGHFHNDNSFATLRDIPGLIVACPARGDDAVELLRSCAALAREDGRVV
                     VFLEPIALYMTKDLHEKHDDLWLAKYPPPGTAAAFGEPRVHHPRAKDLLIVSFANGVP
                     MSLRAARLLEKETGKKTRVLDLRWLKPLNATAIARHAAACKRVVVVDEGRRTGGIGEE
                     IFTILDEHCDDGVKKVRVAADDSFIPLGDAANLVLVQETEILAAARKLLA"
BASE COUNT          552 a         1018 c         1104 g          611 t
ORIGIN      
        1 gcctggaatc cggggacacg gcgcccgtcg tcaagggaaa atcgcgaggc gaaccgcagg
       61 ctggccgcgg agatcgtgag acagggatgg agcttctggc cggcgcgcgg acgcaacgag
      121 aatggtctgc acgttgagga gtcctttgcg gtggagcgca tcgcggccga cgcggcgatc
      181 gcgctcgcgc accgctttca tcaggccgcc gtcttctact gggacggaag cgaggcctgt
      241 attttgcctg cgagtcccgc agggatgacc gccacatgac ggggggaccg gcatgctcga
      301 aatgagaccg aactgcgagt gctgcggacg accgctggcg cccgatgcgc gggcctacgt
      361 ctgctctctc gaatgcacgt tttgcccgag ctgcgcggaa gaaaccttgc gtcgcgtgtg
      421 cccgaactgc gggggagaat tgcggctgca tcccatccgt gccgcgcgcc ttctcgcgcg
      481 ttttccggca tctgcgggaa aaatcgtcaa actagggggc tgcggctaga ctggattact
      541 cgcgagccgc gggacgaaag cctggtattc aggcgaagcc agggaggaac aaggccgtga
      601 attaccgcat tctcgccctc atcggcgtgc gtctgcttgc catctggctg atcgtgctcg
      661 gtatctcggc gttgccggac atccagctga cttggcaggc ggcgatcgca agccaccatc
      721 ccagccaagc ctggatcaca ttcggatatg cgatggtgat gctggcgatc gtggttccgc
      781 tgattctggg agtgtgtctg tgggcgcttg gcccgtggct cgctcggcgg ataacgccgg
      841 cggaagagga tgcgccaccc gacgcgacgg cgccggtcct ggttcagggg gcaatcgcca
      901 tagcgggctt ggtgctcgcg gtcacaactc ttccgggcct gatctttgca atcgtgacgg
      961 ttgtccgtga agccgcacaa ccgttgggca ttcgcgaaca aacatggaca acatttggct
     1021 atcggatgat tctcattctg ctcgcgctgg ggcttgtcgt cggtgcgcgg cgtctgcgcg
     1081 actggatttt ccgtttacgc acaatgggca cctgatggct gcgtcgcgac ttttcttgtc
     1141 agtaggccgg gataagcgaa gcgttcccgg cggatgcgat ttttgcccgc aacgccgctt
     1201 cgcggcttat gcgggcctac gactttgtac gtttcttttt gtcggcggcg ggagaagtct
     1261 caggcgagga gcttgcgtgc ggcggcgaga atttcggtct cctgcacgag aacgagattg
     1321 gcggcatcgc ccagcgggat gaagctgtcg tccgccgcga cccgcacttt tttgacgccg
     1381 tcatcgcagt gttcatcgag gatggtgaag atctcttcgc cgattccgcc tgtgcgccgg
     1441 ccttcgtcca cgacgacaac ccgtttgcag gccgcggcgt gcctggcgat cgcggtcgca
     1501 ttcagcggtt tcagccagcg caaatcgagc acccgcgttt tctttcccgt ttctttctcc
     1561 aatagtcggg cggcccgtag cgacatcggt acgccgtttg caaaactcac gatcaggaga
     1621 tccttcgcgc gcggatgatg gacgcgcggc tcgccgaagg cggcagccgt tcccggcggc
     1681 ggatacttgg cgagccagag atcgtcgtgt ttttcgtgga ggtccttcgt catgtagagg
     1741 gcgatcggtt ccagaaacac gactacccgg ccatcctcgc gtgcgagcgc ggcgcagctt
     1801 cgcaggagtt cgacggcatc gtctccgcgc gccggacagg cgacgatcag gccgggaatg
     1861 tcgcgcagcg tcgcgaagga attgtcgttg tggaaatgtc cgccgaagcc cttttgatag
     1921 gcgagcccgg cgatgcgcac caccatgccg ttggaaaacc gcccgttcga gaaaaactgc
     1981 agcgaggccg cctcgccgcg gagctgatcg agcgcgttgt gcagataggc gaggtactga
     2041 atttcgggaa agggcaggag gcccatcatt gcctggccct gcgcgaggcc gaggatcgtc
     2101 gtttcgtcga gcagcgtatt gaagacgcgg cgcgcgccga acttcgcgtg caggccgctc
     2161 gttaccgtat atacgccgcc cttcgcggcg acgtcttcgc cgaaaagcgt catttcggga
     2221 tacttcgcga gcagctccac cagcgcgcgg ttgatctgaa tcgagagatg gcgcgggggg
     2281 ccatcttccg gaaggcgcgc ggcgccgcca caggcagcct cacgcgccgc ttcgaaatcg
     2341 gtccgctcgg cttccgcttt cacgcctgcc gcgtcgagtg gcgcgagcgc cgccatgacg
     2401 tctcggcgac tcctcaggcg cgggcggttc gccgcccgcc gcgaagcctc ccgcacccgt
     2461 tcgcggcagg cttcgtagcg agcgagcagt gcgccgggag tggtgagccc gagttcgagc
     2521 gccgtttccg ccgaaaagag cagcggatcg cgcgcttcgt cggcttccac cttttcgatt
     2581 ccgcgatatt ccacctccac atccgtgccc gcatgtccga gcaggcgtac ggtgcgcaga
     2641 tgcaggaaga ccggcgcgcg acggctgcgg cagaattcga ccgcttcggc gacggcgccg
     2701 taaccctgcg cgaggtcgag cccgtcggct ttcacataat gcaggcccgg gcgctgcgag
     2761 aaattggcct ccacccatcc cgcgggcgtc tcgacgctga tcccgatccc gttgtcttcg
     2821 cagacgaaga ggatcgggca gggcagtttg cggaacgcgc tttgcgccgc ggcattgaag
     2881 ccggccaatg ccgttgcgtg attcacagag gcgtccccga aggagcagac gacgatgctg
     2941 tcggagggga tcggcagaca cttggcgcgc gccgccggcg cgatcgcgag cgcggcgccc
     3001 accgccttcg gcaaatggct cgcgatcgtc gaggtctgcg gcggaagcca cagcggcgcg
     3061 ctgccgaaga ccttgtgccg accgccggag gccgggtcgt cgctgctcgc ggcgaaggag
     3121 agcgccatgt cgtataccgg atcgagttcc ggcagttgcc gcgcgcgctc gatgaagaac
     3181 gcgccgctgc gataatgcag gaaaccggga tcggttcggc gcgcgagccg gccgagaaga
     3241 acgttgccct cgtgaccagc ggaaccgatc gtatagaaac cttcg
//
LOCUS       DASUJG010000173         3010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_419236, whole genome shotgun sequence.
ACCESSION   DASUJG010000173 DASUJG010000000
VERSION     DASUJG010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419236"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VFX38_03605"
     CDS             <1..196
                     /locus_tag="VFX38_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011596346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX5313980.1"
                     /translation="PGASLLLNQLISQAFVPEYQVRWRLKRNSVAIWDNRCTQHYAVQ
                     DFWPAVRNLERAGIIGDKPF"
     gene            270..1172
                     /locus_tag="VFX38_03610"
     CDS             270..1172
                     /locus_tag="VFX38_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017681912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX5313981.1"
                     /translation="MTAATAAPTVSPISSRGSIKVEPLTCAIGAELSNVNLGAASRDP
                     ELVAEIRALLLQHKVLFFRDQDFSRAEHVAFARHFGELEDHPVAGSDPDHPGLVRIYK
                     SPETPNDRYENAWHTDATWRERPPFGCVLRCVECPAVGGDTTWVNMAEAYKRLPEDVK
                     TRISGLRARHSIEASFGAAMPVEKRLALKAQYPDAEHPVVRTHPETGEKILFVNAFTT
                     HFTNFHSPANVRFGQDFSPGASLLLNYLISQAFVPEFQVRFRWRKNSVAFWDNRSTQH
                     YAVMDYPPCHRKMERAGIIGDVPF"
     gene            1205..2251
                     /locus_tag="VFX38_03615"
     CDS             1205..2251
                     /locus_tag="VFX38_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5313982.1"
                     /translation="MYRHFMVPLDDSPLSAETVNQAVELARALDAKVTFFHAQADYGA
                     SSVGALERVLSPAVFNDHLAGEAQAILAKAEAVARAVGVRCESAVMTSDRPYEAILST
                     AAARGCDLIFIASHGRRGIKGLMLGSETQKVLQNTTIPVLVSAVESNLPTEQQAGPLG
                     VIRDEHRSIAAVIHGLEFLVRDAREKGQPPSFSLLRAILHYIKEFPDSLHHPKEDAYL
                     FRMLRARTSEFDATLDKLQQQHVDGHRWVEELERSIVAYEADPEGGLPLFAKAVERYA
                     TSEMQHMMLETKVIIPAARKHLTVEDWTEIGSAFGGNSDPRFSVDNDEEFRQLFVRIM
                     NLAPVAATGKASGR"
     gene            2248..>3010
                     /locus_tag="VFX38_03620"
     CDS             2248..>3010
                     /locus_tag="VFX38_03620"
                     /inference="COORDINATES: protein motif:HMM:NF018662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 family protein"
                     /protein_id="HEX5313983.1"
                     /translation="MSSGNRGEQQSIQNHTRETELMQRAPRPRVGIRAFRRRTLALAI
                     GSVLATAGTAQAFDIDTGNPDLVMRWDNTVRYNLGWRAQSQDPKILGNPNYDDGDRNF
                     SNGSIVTNRVDVLSAFDLIYQRKFGFRASAAAWADAAYSGLDNKNNATANTLVNGLPV
                     AGALSPYTERYAKGPSGEWLDAFAFANFDVGEVPVSIKAGQHTVYWGDSLLLGGAVHG
                     VSYGQNPLDIWKGFATPGAEAQELFRPRGGITMQAQ"
BASE COUNT          585 a          968 c          947 g          510 t
ORIGIN      
        1 tcccggcgcg agccttctgc tcaaccaact gatcagccag gctttcgttc ccgagtatca
       61 ggtgcgctgg cgcctgaagc gaaacagcgt cgcgatctgg gacaaccgct gcacacagca
      121 ctatgcagtc caggacttct ggccggcggt acgcaatctc gagcgcgccg ggatcatcgg
      181 cgacaagcct ttctagcgaa ggcgtcgacc gatcgggcag cgattgccgc gggtatcgcc
      241 gccgcaacat tttggagagg aatgcagaca tgacagccgc aaccgccgca ccgaccgtct
      301 cgcccatcag ctcgcgcggt tcgatcaagg tcgagccgct cacctgcgcg atcggcgcgg
      361 agctcagcaa cgtgaatctg ggcgccgcct cacgcgatcc ggaactggtc gccgaaattc
      421 gggcgctcct gctccagcac aaggtcctgt tcttccgcga ccaggatttc tcgcgcgccg
      481 agcatgtcgc attcgcccgg catttcggcg aactcgaaga tcatccggtt gccggcagcg
      541 atccggacca tccagggctg gtgcgcatct acaagtcacc ggagacgccg aacgatcgat
      601 acgagaatgc ctggcacacc gacgcgacct ggcgcgagcg accgccattc ggctgcgttc
      661 tccgctgcgt cgaatgccca gcggtcggcg gtgacacgac gtgggtcaac atggccgagg
      721 cctacaagcg actccccgag gacgtcaaga cgcgaatctc gggactgcgc gcgcgccaca
      781 gcatcgaggc gagcttcggg gcggcgatgc ccgtcgagaa gcgcctggcg ctgaaggctc
      841 aataccccga tgcggagcac ccggtcgtgc ggacgcatcc agagacaggc gagaagatcc
      901 tcttcgtcaa cgccttcacc acgcacttca cgaactttca cagtccggcg aacgttcgct
      961 tcggacagga cttcagcccg ggagcgagcc tgctgctcaa ctacctcatc agccaggctt
     1021 tcgttcccga gtttcaggtt cgctttcgct ggcggaagaa cagcgtcgcg ttctgggaca
     1081 accgctcgac gcagcactac gccgtcatgg attacccgcc gtgtcatcgc aagatggaac
     1141 gggcgggaat catcggcgac gtgccctttt gaacggcagc cgctaggccg tcctggagaa
     1201 ccgcatgtat cgccacttca tggtcccgct cgacgactcg ccgctctcgg cggaaacagt
     1261 caaccaggcc gtcgagctcg ctcgggcgct cgacgcgaag gttacgttct ttcacgcgca
     1321 ggcggattac ggcgcatcga gcgtcggcgc gctcgagcgc gtgctttcgc cagcggtgtt
     1381 caacgatcac ctggccggtg aagcacaggc gatcctggcc aaggccgaag ctgtggcgcg
     1441 ggcggtgggt gtgcgctgtg aatccgcggt gatgacgagt gaccggccct atgaagcgat
     1501 tctgagcacc gccgccgcac gaggatgcga cctgatcttc atcgcttcgc acgggcgccg
     1561 gggcatcaag ggcctcatgc tcggctccga gacgcagaag gtactgcaga acacgacgat
     1621 tcccgtgctc gtgtcggccg tcgagagcaa ccttcccacc gagcagcagg ccggaccact
     1681 gggggtcatc cgcgacgagc atcgctcgat cgcggcggtg attcacggcc tcgaattcct
     1741 cgtgcgcgat gcgcgcgaaa aggggcagcc gccgtcgttt tcgcttctgc gcgcgatcct
     1801 gcattacatc aaggaatttc cggattcgct gcaccacccg aaggaggacg cctacctgtt
     1861 ccgcatgctc cgggcgcgta cgtcggaatt cgacgcgacg ctcgataagc tgcagcaaca
     1921 gcacgtcgat ggccaccggt gggtcgagga actcgagcgc agcatcgtgg cttacgaagc
     1981 tgatcccgaa ggcgggttgc cgctgttcgc caaggcggtc gagcgttacg caacgtcgga
     2041 gatgcagcac atgatgctgg aaacgaaggt catcatccca gcggcgcgca agcacctcac
     2101 ggtcgaggac tggacggaaa tcggaagcgc ttttggcggt aacagcgatc cgcgattctc
     2161 ggtcgacaat gacgaggaat ttcgccagct cttcgtgcgc atcatgaacc ttgctccggt
     2221 tgccgccacc ggcaaggcga gcggtcgatg agcagcggca atcggggcga gcagcagagc
     2281 attcaaaacc acaccaggga gactgaactc atgcaacgtg caccaagacc acgcgtcggc
     2341 atccgggcct tccggcgccg cacactcgcg ctcgcgatcg gttcggtact cgcgacagcc
     2401 gggacggcgc aggcgttcga tatcgacacc ggcaaccccg acctcgtgat gcgctgggac
     2461 aataccgtcc gctacaacct gggctggcgg gcacagtcgc aggatccaaa gattctgggc
     2521 aatccgaact acgacgacgg cgaccgcaat ttctccaacg gttcgatcgt caccaatcgg
     2581 gtggacgtct tgtccgcgtt cgacttgatc tatcagagga agtttggctt tcgcgcgagc
     2641 gcggcggcgt gggcggacgc tgcgtacagc ggtctcgaca acaagaacaa tgcgaccgcc
     2701 aacacgctgg tcaacgggct gcccgtcgcc ggagccctga gcccgtatac cgagcgctat
     2761 gcaaaggggc cctcagggga atggctcgat gccttcgcgt tcgcgaactt cgacgtgggt
     2821 gaagttcctg tcagcatcaa ggcggggcag cacaccgtgt attggggtga cagcctgctt
     2881 ctgggcggcg cggttcacgg cgtgtcgtac gggcagaatc cgctcgacat ctggaaaggc
     2941 ttcgcgacgc cgggcgccga agcgcaggag ctattccgcc ccaggggtgg gatcaccatg
     3001 caggcgcaac
//
LOCUS       DASUJG010000174         2523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_909075, whole genome shotgun sequence.
ACCESSION   DASUJG010000174 DASUJG010000000
VERSION     DASUJG010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909075"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VFX38_03625"
     CDS             complement(<1..483)
                     /locus_tag="VFX38_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5313984.1"
                     /translation="MFRKFFAFLVLLSGAVCLPAFAAGASPAYFALHDLGHGVWAAIS
                     PPTSKAGANAGFVIGPQGVLVIDTFEDPAAAEAMLAAIREKTDAPIRYVVDTHYHLDH
                     VAGNNVFAAAGAVVMAQQNVRTWERTQNLKFFGQHPTAEQQKFVASLGLPTVCYTQDV
                     T"
     gene            721..1563
                     /locus_tag="VFX38_03630"
     CDS             721..1563
                     /locus_tag="VFX38_03630"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HEX5313985.1"
                     /translation="MSPSRVPDGGERGWIVPIGGAEEKTSSPKILQRFVALCGGEASN
                     IVVIPTASRLGETGAGYRKVFGELGAAAVEVLDFDTRRDCHEPSRLAIIERASGVFFT
                     GGNQMRLSSMIGGTPVAKAIRARNAAGMPVAGTSAGASILSDHMIAFGEEGSSPRADS
                     VHLAPGLGLTNRFIIDQHFRQRDRLGRLVAALAYNPFPVGLGLDEDTAAFIGPDRTLE
                     VEGSGTVTVVDASGLQFSSMDRVAEHEPVCLLGLTVHILTAGATFNLETRRASAGSLA
                     TNKS"
     gene            1580..>2523
                     /locus_tag="VFX38_03635"
     CDS             1580..>2523
                     /locus_tag="VFX38_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX5313986.1"
                     /translation="MRILNRSVYVGPSQYARFPVIRLELDLGELEQWPTGRLGSAFVN
                     ALLKELPGLAEHGCSYGEPGGFIRRMREGEGTWLGHVFEHVAIELQNVAGEQVTFGKT
                     RGVGEERPGVYRVIYQYEQREEGIAAGELALSLLVSLLPENLRPPELLPADWNWESER
                     DRFIKYAQRRALGPSTASLVRAAEERDIPWFRLNEYSLIQLGHGKYQQRIQATITGRT
                     SHIAVELAGDKEETNRILAAQGLPVPHQYRVTDAAGALRAARRLETPVVVKPYNGNHG
                     RGITINLTADEDIRAAFEAAREHSRSVIVETYISGDDHR"
BASE COUNT          434 a          835 c          850 g          404 t
ORIGIN      
        1 cgtcacgtcc tgcgtgtagc agaccgtcgg caggccgagc gatgcgacga acttttgctg
       61 ctcggccgtg ggatgctggc cgaagaactt gaggttctgc gtacgttccc aggttcgtac
      121 gttctgctgc gccatcacca ccgctccggc cgcggcgaag acgttgttcc ccgccacgtg
      181 atcgaggtga tagtgcgtgt ccacgacgta gcggatcggc gcgtcggttt tctcgcggat
      241 tgcggccagc atcgcctcgg ccgccgccgg atcctcgaag gtgtcgatca ccagcacgcc
      301 ctgcggaccg atgacgaagc ccgcgttggc acccgccttg ctcgtcggcg gtgaaattgc
      361 cgcccatacg ccgtgaccga ggtcgtgcag cgcgaagtag gccggggacg cgccggcggc
      421 gaaagcgggc aggcagaccg ccccgctcag aagaacaagg aacgcgaaga acttccggaa
      481 cacggcacac ctccattccg gcgacgccat cgcgcgccgc cctcgaaaat gccgaaccat
      541 ggtaccgcca tccgcgcgaa cgagcacgcg ggcgcgtacg cgtgggcggg cgatgcgatc
      601 cggtggcgat gtggaaattt tggagcaagc cggtacaatt cgcgtgcctg ccgcggggcg
      661 cgccgttcac gtcgccacgc caaggcagtt cgagacggat cacgacaagg agaccaatgc
      721 atgagtccga gcagggttcc cgatggcggg gagcgcggct ggatcgtacc gatcggcggc
      781 gccgaggaga agaccagcag cccgaagatt ctgcaacgct tcgtcgcact ctgcggcggc
      841 gaggcgagca acatcgtggt gattcccacc gcgagccgtc tcggggaaac cggtgcgggc
      901 taccgcaagg tcttcggcga actcggggcg gccgcggtcg aggttctgga tttcgatacc
      961 cgtcgggatt gccacgagcc gagccggctc gcgatcatcg agcgcgcgag cggggtgttt
     1021 ttcaccggcg gcaaccagat gcgcctttcg tccatgatcg gcggcacgcc ggtcgcgaag
     1081 gcgatccgcg cccgcaatgc cgcgggcatg ccggtcgcgg gaaccagcgc cggcgcgagc
     1141 attctgagcg accacatgat cgccttcggc gaggagggat cgtcgccccg cgccgacagc
     1201 gtgcatctcg cgccgggtct cggcctcacc aaccgcttca tcatcgacca gcatttccgc
     1261 cagcgcgacc gcctcggccg gctggtcgcg gcgcttgcct acaacccgtt tccggtcggt
     1321 ctcggactgg acgaggacac cgccgccttc atcggcccgg accggacgct ggaggtcgag
     1381 ggcagtggta cagtgaccgt cgtggatgcg agcggcttgc agttttcctc gatggaccgg
     1441 gtggccgaac acgagccggt gtgcctgctg ggtctgacgg tccatatcct cacggcgggc
     1501 gcgaccttca acctggaaac gcgccgcgcc tcggccgggt cgctcgccac caacaagagt
     1561 tagcctgccg agagtcgtga tgcgtattct caatcgctcg gtttatgtcg gcccgtcgca
     1621 gtacgcgcgc tttccggtca ttcggctgga gctggatctc ggggaactgg aacagtggcc
     1681 gaccgggcgg ttggggtcgg cgttcgtgaa tgcgttgctg aaggagctgc ccggcctcgc
     1741 cgagcacggc tgctcgtatg gcgagccggg aggattcatc cggcgcatgc gcgaaggcga
     1801 aggaacctgg ctcgggcatg tattcgaaca tgtcgccatc gaactgcaga acgtcgccgg
     1861 cgagcaggtg accttcggca agacgcgcgg cgtcggcgag gagcggcccg gcgtgtaccg
     1921 ggtgatctat cagtatgagc agcgcgaaga aggcatcgcg gccggcgagc ttgcgctgag
     1981 tctccttgtc tcgctcctgc cggaaaatct gcgcccgccg gagctgctgc cggcggactg
     2041 gaactgggag agcgagcgcg accgcttcat caagtatgcg cagcgccgcg ccctggggcc
     2101 gtccaccgcc tcgctggtgc gcgcggcgga ggagcgcgac attccctggt tccggctcaa
     2161 cgaatattcg ctgatccagc tcggtcacgg caaatatcaa cagcgcatcc aggcgaccat
     2221 caccgggcgc acctcgcaca tcgcggtcga acttgccggc gacaaggaag aaaccaaccg
     2281 catcctcgcg gcacagggcc tgcccgtgcc gcatcaatac cgcgtcaccg acgccgccgg
     2341 cgccctgcgc gcggcacggc ggctggaaac tccggtcgtc gtcaagccct acaacggcaa
     2401 ccacggtcgc ggcatcacca tcaacctcac cgcggacgag gacattcgcg ccgccttcga
     2461 ggccgcgcgc gagcattccc gttcggtaat cgtggagacg tacatttccg gcgacgatca
     2521 tcg
//
LOCUS       DASUJG010000175         2479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_664892, whole genome shotgun sequence.
ACCESSION   DASUJG010000175 DASUJG010000000
VERSION     DASUJG010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664892"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1398
                     /locus_tag="VFX38_03640"
     CDS             <1..1398
                     /locus_tag="VFX38_03640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HEX5313987.1"
                     /translation="LGGGGWVGRADILRRIEEPSVLGAWSYEPIDTKLARETRAGSVL
                     QLCLYADLLTAMQGLAPAHLYVIVPWSEFEPQHYRFADFAAYFRKVKNGLLASLAAEA
                     PQDTYPDPIEHCDICRWQATCDKRRRDDDHPSLVAGISKMQINELKGHGITTVEAVAA
                     LPLPLPWKPERGSAESYVRVREQARIQIEARASGERRFELLPVETGFGLTRLPEPSPG
                     DVFLDLEGDPFAGDGGFEYLFGYEVAGDDAAGTYNGDWAFTREAEKKAFEEFVDFVIA
                     RWEQFPSLHVYHYAPYEPAALKRLMGRYATREEEIDRMLRAGIFVDLFQVVRGAVRAS
                     VESYSIKKLEPFYDFVRDTNLADANVALANLQAGLELDNIPSITEDTKATVLGYNRDD
                     CRSAAALRDWLEDQRGQIVAGGTEVPRPAAGEDEGAPSEKIAEWLMRITPLIEKLCAG
                     VPADPDKRNEDQQGR"
     gene            1411..>2479
                     /locus_tag="VFX38_03645"
     CDS             1411..>2479
                     /locus_tag="VFX38_03645"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5313988.1"
                     /translation="MLDWHRREEKAVWWEYFRLADLSAEDLLDERAGLSGLSYVGEAG
                     GTTKAPIHRYSFVAQETELRGGEDLRNLGGARFGKLELISFEERSVDIKKRQDSAAIH
                     APAVFAHTHVPSDVIAEAIVRIGTHVAESGLQGDGPYRAARDLLLRHGPRVAGEPLRD
                     NGETAVQAVDRLCEHLDGGILPIQGPPGAGKTFTGAQMICELVRQGKTVGITANSHKV
                     IRNLIDETIRVADEQGVDVRCCHKADVAQEEQHRLSFAKRSEDLIAALGHGVSVGGGT
                     AWLWSRPDAFEAVDVLFVDEAAQMTLARVLAVSQAAKTVVLIGDPQQLDQPLQGSHPE
                     GTEVSALDHILAGEHTIPADKG"
BASE COUNT          477 a          747 c          821 g          434 t
ORIGIN      
        1 ctgggaggtg gcgggtgggt cggtcgggcc gacatcctgc ggcgtataga agaaccgagt
       61 gtgctcggcg cgtggtccta cgaaccgatc gatacgaagc ttgctcgcga gacccgggcc
      121 ggcagtgtcc ttcaactttg cctgtatgcc gatttgctga ccgcgatgca gggccttgcg
      181 cctgcgcatc tctacgtgat cgtaccgtgg tcggaattcg agccgcagca ttatcgcttc
      241 gcggacttcg cggcctactt ccgcaaagtg aagaacggat tgctcgcatc cctcgccgcc
      301 gaagcgccgc aagacaccta ccccgatccg atcgagcatt gcgacatttg ccgctggcag
      361 gcgacatgcg acaagcgtcg ccgcgacgac gatcacccgt ccctggtggc ggggatttcg
      421 aagatgcaga tcaatgagct taaagggcac ggcattacga ctgtcgaggc cgtcgccgcg
      481 ctgcccttgc ctttgccttg gaaacccgaa cgcggctccg ccgagtccta tgttcgcgtt
      541 agagaacagg cgcggataca aatcgaggcg cgcgcgagcg gcgaacgccg gttcgagttg
      601 ctgccggtcg aaaccggctt cgggctcacc cgtcttcccg agccttcgcc gggcgacgtc
      661 ttcctcgatc tcgaaggcga tccgttcgcc ggcgatggcg ggttcgaata cctgttcggc
      721 tacgaggttg ctggcgacga cgccgccggg acgtacaatg gcgattgggc tttcacgcgc
      781 gaagccgaga aaaaggcttt cgaagaattc gtcgattttg tgattgcccg atgggagcag
      841 ttcccaagcc ttcacgtcta tcattacgct ccctatgagc ccgccgcgct caaacgcctg
      901 atgggccggt acgcgacgcg cgaagaagaa atcgaccgga tgcttcgcgc ggggatcttc
      961 gtcgatctgt tccaagtggt gcgcggcgcc gtgcgtgcca gcgtcgaaag ctattcgatc
     1021 aagaagctcg agccgtttta cgactttgtg cgcgacacga acctcgcgga cgccaacgtc
     1081 gctctggcga acctccaggc cggactcgaa ctggacaaca ttccctcgat cacggaggac
     1141 actaaggcga cggtccttgg atacaacagg gacgattgtc ggtcggccgc tgcgctgcgg
     1201 gattggctgg aagaccagcg cggtcagatc gtcgcgggcg gcacagaagt cccgcgtccc
     1261 gccgccggcg aagacgaagg tgcgccgagc gaaaaaatcg ccgagtggct gatgaggatc
     1321 acgccgctga ttgaaaaatt gtgcgcaggc gttccggcgg atccggacaa gcgcaatgaa
     1381 gatcagcaag ggcgctagct tctggcgaac atgctcgact ggcaccgccg ggaggaaaag
     1441 gccgtgtggt gggaatattt ccggcttgcc gatctctcgg ccgaagactt gctcgacgag
     1501 cgggccggcc tgtccggcct gagctacgtc ggcgaagcgg gaggcacgac gaaggcgccg
     1561 atccaccgct acagctttgt cgcgcaggaa acggagctgc gcggcggcga ggatctgcgc
     1621 aatctcggcg gcgcgaggtt tggcaagctc gagctgatct cgtttgagga acgctcagtg
     1681 gacatcaaga agcggcagga cagcgccgcc attcacgcgc cggcggtatt tgcccatacg
     1741 catgtgccgt ccgacgtgat cgccgaagcc attgtgcgca tcggtacaca cgttgccgaa
     1801 agcggtttgc agggtgacgg tccctaccgg gctgcgcgcg atctgctgct gcggcatggc
     1861 ccgcgggtag caggggagcc gctgcgcgat aacggcgaaa cggcggtgca ggcggtcgac
     1921 cggctatgcg aacacctcga cggcggcatt ctcccgatcc aaggaccgcc cggcgcgggc
     1981 aagaccttca ctggcgcgca gatgatttgt gagctggtgc ggcagggcaa gacagtcggg
     2041 atcacggcca acagccacaa ggtcattcgc aacctcatcg acgagacgat cagggtcgcc
     2101 gacgagcagg gagtcgacgt ccgttgctgc cacaaggccg acgtggctca agaggaacaa
     2161 catcgcttga gcttcgccaa acgcagcgag gatctgatcg ccgcgctcgg gcacggcgtt
     2221 tcggtcggtg gcggcacagc ctggctgtgg tcgcggccag acgcgttcga agccgtcgat
     2281 gtgttgttcg tcgatgaagc ggcgcaaatg acgctcgcac gggtgctcgc tgtatcgcag
     2341 gccgcaaaga ctgtcgtgct gatcggcgat ccgcagcagc tcgatcagcc gctacagggc
     2401 agccaccctg aaggcacgga ggtgtcggcg ctcgatcata tccttgccgg agagcacacg
     2461 atcccggcgg ataaggggc
//
LOCUS       DASUJG010000176         2407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_542371, whole genome shotgun sequence.
ACCESSION   DASUJG010000176 DASUJG010000000
VERSION     DASUJG010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542371"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            349..570
                     /locus_tag="VFX38_03650"
     CDS             349..570
                     /locus_tag="VFX38_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5313989.1"
                     /translation="MSILSPTAFILQRERDGKVFRLVDGSPQNLAPLVEASVGSRGQH
                     SLARISIEAPRSIEILSGELYREKYPNRA"
     gene            685..1845
                     /locus_tag="VFX38_03655"
     CDS             685..1845
                     /locus_tag="VFX38_03655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5313990.1"
                     /translation="MITRFTTVYAGHVDLPDRGQNETPANERHFSNEHLATVFSKSEH
                     IARTMDQLGYDTVWFAEHHFQREGYECVPNIPLLAVHLAHLTKRIKLGCAFNIAPMWH
                     PLRLAEDFATADILTGGRTVFGVGRGYHAREVETFGAPMLDQSANRELFEEQVEVILK
                     AFNEESFSHKGKHYTLPPEVPYRGYTLKELTLVPRPVHRPVEIWQPIVSANPRGLEFM
                     VRNGIKGAVGGGAATLEAGPITGYRDAAARLGKELQLGENLQLGLHLHIARTEEQAIR
                     EITPIFEEHVKMFAPLGFVPGLTPEQVAAVARRGGWAAAGIPTVDHYAKSGAWFAGTA
                     AQLVEHLKGLEAKYPGLEYVSLSSPLGTPESVMIEQLHWIAEEVMPQFKPRT"
     gene            2130..>2407
                     /locus_tag="VFX38_03660"
     CDS             2130..>2407
                     /locus_tag="VFX38_03660"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5313991.1"
                     /translation="MRPITAGIGTLRRIVGLAVPAIALILGGTAASYAASPIVIGGTV
                     SETGALAEDAEFQVKGIELAIADANAHGGWLGRKLDFKVYDDKSNAGTA"
BASE COUNT          426 a          810 c          762 g          409 t
ORIGIN      
        1 agcggcggcg ttcctagcga tttctgccct tcgctccggg ctgagcttgt cccggcgcgc
       61 ggccccaccc ttcctgccga gcgcctgtgc gcccttgtcc ttacccgaat cgcccatgtc
      121 ctcctccgcc tcccccgtga gaatcttggc gacgcggatc gcgtttgaca cgacatccgc
      181 ggggcgcttc tggccttgtg gtcctctcgg cataccggca tccttgggaa tgcttaacgt
      241 caagcacata taggccgtag tgtctcagtt tgcgaggatg tcatggcatt agtgctagag
      301 cgcagatcgg gcgaagattt ttatgtgggg cgcgagaggt tcatcctgat gagcattctt
      361 agcccaacag ccttcattct tcagcgggaa cgcgacggca aagtgtttcg cctggtggac
      421 ggatcgcccc aaaaccttgc tcccttggtt gaagcgagcg tcggttcaag gggccaacat
      481 tcgctcgccc ggatttccat cgaagcgcct cgctctatcg agattctgag cggcgagctt
      541 taccgtgaaa agtatcccaa ccgcgcgtag ggcggcattt caaactgagt cactacccat
      601 cagcggctca cggggcgtgg caaacggctc ggggttgggt cacactggcc cgcagcatcg
      661 actatggatt cggaggaagg acgcatgatc accaggttca cgaccgtcta tgccggccat
      721 gtcgacctgc ccgaccgcgg ccagaacgag acaccggcca atgagcggca cttttccaac
      781 gagcacctcg ccaccgtgtt cagcaagagc gagcacatcg cgcgcacgat ggaccagctc
      841 ggctacgaca ccgtctggtt cgccgagcac catttccagc gcgagggcta tgagtgcgtc
      901 cccaatatcc cgctgctggc ggtgcacctc gcgcatctga ccaagcggat caagcttggc
      961 tgcgccttca acatcgcgcc gatgtggcac ccgctgcgcc tcgccgagga tttcgcgacc
     1021 gccgacatcc tgaccggcgg gcgcaccgtg ttcggggtcg ggcgcggcta tcacgcccgc
     1081 gaggtcgaga ccttcggcgc cccgatgctc gaccagagcg ccaaccgcga gctgttcgaa
     1141 gagcaggtcg aggtgatcct gaaggcgttc aacgaagaga gcttctcgca caagggcaag
     1201 cactacacgc tgccgcccga ggtgccctat cgaggctata cgctgaaaga gctgaccctg
     1261 gtgccgcgcc cggtgcaccg ccccgtcgag atctggcagc cgatcgtcag cgccaacccg
     1321 cgcggcctcg aattcatggt gcgcaacggt atcaagggtg cggtcggcgg cggcgccgcg
     1381 acactcgagg caggcccgat caccggctac cgcgacgccg cggcgcggct tggcaaggag
     1441 ttgcagctcg gcgagaattt gcagctcggg ttgcacctgc acatcgcgcg caccgaggag
     1501 caggcgatcc gcgagatcac cccgatcttc gaggagcacg tcaagatgtt cgcgccgctc
     1561 ggctttgtgc cggggctgac gccggagcag gtcgccgcgg tcgcgcgccg cggcggctgg
     1621 gccgccgccg ggataccgac ggtcgaccac tacgccaaat ccggtgcctg gttcgccggc
     1681 accgccgcgc agctggtcga gcacctgaag ggcctggagg cgaaatatcc cggcctcgaa
     1741 tatgtcagcc tgtcgagccc gctcggcacg cccgaaagcg tcatgatcga gcagctgcac
     1801 tggatcgccg aagaagtcat gccgcagttc aagccgcgga cctagtcccg tatgcaaccc
     1861 gtcatcccgg ccaagacgct gggcgttggc ggcggcgcat cggccggcca gggggttagt
     1921 tggctagggc gagaagtcgc acctgcgccg gcgaccgcgc gcggcggttg atcacctcgt
     1981 gcggaacgat tagcgtccgc tcccgttcct cgccgcgccc cgcccgcact cgcgtctccg
     2041 gagcgctgct tttccggccg aaatcgccag actcgtcagc cgcaaggtcg acaaggatcg
     2101 cgggaggcaa gcccccagga ggcaaaagga tgcgtccaat caccgcagga atcggcacgc
     2161 tgcgccgcat tgtcggcctc gccgtgccgg caatcgcgct cattctcggc ggcaccgcgg
     2221 cgagctatgc cgcgtcgccg attgtcatcg gcggcacggt gtcggaaacc ggcgccctcg
     2281 ccgaggacgc cgaattccag gtcaagggca tcgagttggc gatcgccgac gccaatgccc
     2341 atggcggctg gctcgggcgc aagctcgact tcaaggtcta tgacgacaag agcaatgccg
     2401 gcaccgc
//
LOCUS       DASUJG010000177         5536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1032465, whole genome shotgun sequence.
ACCESSION   DASUJG010000177 DASUJG010000000
VERSION     DASUJG010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5536
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032465"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(619..2847)
                     /locus_tag="VFX38_03665"
     CDS             complement(619..2847)
                     /locus_tag="VFX38_03665"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HEX5313992.1"
                     /translation="MRRLLIVLSIALAPLAAAAATPAPAAATALAPQARQVLIARTVV
                     EVMQSKHYPAKPLDDKFAQALLDQYFDDLDPGRFYFTQADIDRFHRYDGELADDLKHG
                     NLEPAFAIYRVYARQVRRQIQDALQLLASEPALGGDETYQFTQPHSPWPKDQKALDEA
                     WQKRATNDVITLMLAGKNWDETKKVLTKRYQYVLNVVNRTTSNDVFDAFMNAYAETQD
                     PHSAYFSPFEAQQFQIAMSLHLEGIGAQLSDSDGYVTVVRILPGGPAAKSGELKSGDR
                     IVGVGEGAKGKIVDVVGWRLDDVVKKIRGPKGSAVRLEVLPAGVLPGGMEKTITFIRN
                     TIELEAERASAKTMFVKRGDTGYRIGVITIPSFYLNFQAEDDDDQNYASVSRDVAALV
                     TELKKGKVSGILLDLRGDGGGSLEEAAAVTGLFIPSGPVVQVQDRSGRVQQLGTPRGE
                     KIVWDGPLAVLVDRFSASATEIFAGALKDYQRALVLGSPTWGKGTVQQLLDLDNYLPG
                     FKAGEVKLTTAQFFRVNGSSTQRKGVLPDIALPSAIDDSQFGEATYPNALPWKRIAPA
                     DYAPVNDAVDATLAGVERYFETTVKDGPRFQLYQKEVAIERAMAARDTVALNLEKRQS
                     ERAAERSQKLQLDNAWRKLNGHKPFTSLKQAGADSFSPPDVALEASGDILGEYIALAA
                     PLVSRFHPAPAASAAAPDLCLTWSATERAPTPVAQPCKQPPAQAATAASATRPRAAGS
                     GG"
     gene            complement(2921..4690)
                     /locus_tag="VFX38_03670"
     CDS             complement(2921..4690)
                     /locus_tag="VFX38_03670"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEX5313993.1"
                     /translation="MTTTNRALADWVEEVARLTKPDKVAWCDGSADEYRRLVAGMLAS
                     GTLLELNQRSFPGCYLHRSNPSDVARVEHLTFICCKNEADAGPNNHWMAPGEAHAKVD
                     PLFDGAMKGRTLYVIPYCMGPIDSPHARLGVEITDSPYVVANMQIMTRMGAAALARIE
                     RGDPFVRGLHSTGDLSPDRRFILHFPEERLIKSIGSGYGGNALLGKKCHALRIASWQA
                     RDEGWLAEHMLIVGIENPQGEVRYVAAAFPSACGKTNLAMLIPPAAYPGWKIWTVGDD
                     IAWLHVGEDGRLWAINPEAGYFGVVPGTNEKTNKNAYETIRKDTIFTNVAVTADKEPW
                     WEGKKTGEPVTDWQGRPYDPANGPAAHPNARFTVSAKQNPSWSPQAEAAQGVPISAIF
                     FGGRRAEVAPLIYEAKSWEHGVLVGAGMASETTAAATGKVGTVRRDPMAMKPFCGYNF
                     ADYWTYWLSFARRAKHPPRIFHINWFRKDEDGNFLWPGFGENLRVLRWALERCDGDAD
                     AEETAIGFLPTADAIDTEGLAVDRATMEALLAVDVDQWREEYAAIGEYLEGYGERLPE
                     GLLREHRRVSEALETAVEKRAHA"
     gene            4789..>5536
                     /locus_tag="VFX38_03675"
     CDS             4789..>5536
                     /locus_tag="VFX38_03675"
                     /inference="COORDINATES: protein motif:HMM:NF018444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5313994.1"
                     /translation="MSLAEESVALLGSGGLLCDRVPGFVPREGQRALAGAIAAAIEAG
                     EHLLAEAGTGIGKTFAYLVPILLGGRRALISTATRALQDQLFERDLPLVARALGRPLD
                     VVVLKGRRNYLCRERWLRLAQDWVGVAAGGIETELLAAWAAQTKTGDLAELPREGNPA
                     VIAGLLTVGAEACLGKACPEFSRCHVYAARRRALEADVVIVNHNLLVADIALKAEGGG
                     DLLGGADVVVVDEAHALPEVARLNLGETLSR"
BASE COUNT          888 a         1774 c         1853 g         1021 t
ORIGIN      
        1 gcctctccgg aggcgccggc ggccggcgca cccgcaccgg cggccggcac ggcgcccgcc
       61 gtaccgcaga gaccgcgact gttgtttcgc agccagccgt tgagcgcagc gagctgatcc
      121 gaatcgagcc ggtcgaggcc ggcctgatcg aaggatgaca cggtcatgaa gtcccgaata
      181 tcaattgcct gcggacatat cgcggatgtt gcagaaggat tgccgtttgc gggagccgga
      241 cagagaccaa ctccgttgtt ttgcaaccac cggttcagac tcgcgagctg tccgccgccc
      301 agatgatcga gccccgactc gtcgaacgcc gcgacggtca tcgattgacg caggtcgagg
      361 gaatcgcagg acgggcccgc aagagccgac gcggccgcaa acaccgctgc catcaggccg
      421 caggccagca gacccgcgcc gcttatgacg cgcaaacgcc gcattgtccc gctacgtcca
      481 ttgtgcttct acccagacgg acgcaaagca tagcattccc gccggcgtcc gcaaaccctg
      541 cctgccgcca tccgcgcacc ccgcggcgaa cccgcgggcg cggcggcgtc gccggcaagc
      601 accaggcggc gagcctcact atccgccgct tccggcggcc cgcggcctcg tcgcgctcgc
      661 cgccgtggcg gcctgtgcgg gcggctgctt gcagggctgc gcgacgggtg tcggcgcccg
      721 ctcggtcgcg ctccaggtca aacagagatc gggggccgcg gcgcttgctg cgggcgccgg
      781 gtggaaacgt gagacgagag gtgcggcgag ggcaatgtac tcgccgagga tgtcgccgct
      841 agcctcgagc gccacgtcgg gcggcgagaa ggaatcggcg ccggcctgct tgaggctcgt
      901 gaacggcttg tgaccgttga gcttgcgcca ggcattgtcg agttgcagct tctgagagcg
      961 ttccgcagcg cgctcgctct gccgcttctc gaggttgagc gcgacggtgt cgcgcgcagc
     1021 catcgcacgt tcgatcgcga cttctttttg atacagttgg aaacgcgggc catccttgac
     1081 ggtcgtctcg aagtagcgtt cgactcccgc gagcgtcgca tcaaccgcgt cgttgaccgg
     1141 agcatagtcg gcgggggcga tccgcttcca cggcagcgcg ttcggatagg tcgcctcacc
     1201 gaattgcgag tcgtcgatcg cggagggcag ggcgatgtcg gggagcactc ccttgcgctg
     1261 cgtgctcgag ccgttgacgc ggaagaactg ggcggtcgtc aatttgacct cgcccgcctt
     1321 gaaccccggc aggtagttgt cgaggtcgag cagttgctgc acggtcccct tgccccaggt
     1381 cggggagccg agaaccaggg cgcgctggta atccttgagg gctccggcga aaatttctgt
     1441 cgccgaggcg ctgaagcggt ctacgagaac agccagcggc ccgtcccaga cgatcttttc
     1501 gccccggggc gtgccgagtt gctgcacccg gccgctgcga tcctgcacct ggaccacggg
     1561 gccgctgggg atgaaaagcc cggtaaccgc ggctgcctct tcgagtgagc cgccgccgtc
     1621 gccgcgcaga tcgaggagaa tccccgagac cttgcccttt ttcagttcgg tgacgagggc
     1681 ggcaacatcg cgcgaaacgc tcgcatagtt ctggtcgtcg tcatcctcgg cctggaaatt
     1741 cagatagaac gagggaatcg tgatgacccc gatgcggtag ccggtgtcgc cgcgcttcac
     1801 gaacatcgtt ttcgcgctcg cccgttccgc ctcgagttcg atggtgttgc ggatgaaggt
     1861 gatcgttttt tccattccgc ccggcagcac ccccgccgga agcacttcga ggcgcacggc
     1921 ggaacccttg gggccgcgaa tcttcttgac cacgtcatcg agcctccatc cgacgacgtc
     1981 gacgatcttg cccttggctc cctcgccgac gccgacgatg cgatcgccgg atttgagttc
     2041 gccgctcttg gcggcgggac cgccgggaag gatgcggacc accgtgacgt agccgtcgct
     2101 atcgctcaat tgcgcgccga tgccctccag atgaaggctc atcgcgatct ggaattgctg
     2161 cgcctcgaac ggcgagaagt atgcgctgtg cggatcctgc gtctcggcgt aggcgttcat
     2221 gaaggcatcg aagacgtcgt tcgaggtcgt gcgattgacg acgttgagca catactgata
     2281 tcgcttcgtg agcaccttct ttgtctcgtc ccagttcttg ccggcgagca tgagggtgat
     2341 cacgtcgttg gtcgcacgct tttgccaggc ctcgtccagc gccttctggt ccttcggcca
     2401 gggagagtgg ggctgcgtaa actgataggt ttcgtcgccg cctagcgccg gttcgctcgc
     2461 gagcagttgc agggcgtcct gaatctggcg gcgcacctgg cgggcgtaga cgcgatagat
     2521 cgcgaaggcg ggttccagat tcccgtgctt gagatcgtcg gcgagttcgc cgtcgtagcg
     2581 gtggaaacgg tcgatgtcgg cctgggtaaa gtaaaagcgc cccggatcga gatcatcgaa
     2641 gtactgatcg agaagcgcct gggcgaactt gtcgtcgagc ggcttggcag gatagtgctt
     2701 gctttgcatt acctccacca cggtgcgggc gatcagcacc tggcgcgcct ggggagcgag
     2761 cgccgtggcc gcggccggag ccggggtcgc ggcggcggcc gcgagcggcg cgagcgcaat
     2821 ggacagaaca atcagaagtc gacgcatacg ggtcctcgtg gtttcaacgg atcgccacgg
     2881 tgagtaccgg gcgccgggag ttggatgatc aggcggaaaa tcaggcgtgc gcgcgtttct
     2941 cgacggccgt ttcgagcgcc tcgctcacgc ggcggtgctc acggagcaga ccctcgggca
     3001 gacgctcgcc gtaaccttcg agatattctc cgattgcggc atattcctcg cgccactgat
     3061 cgacgtccac ggcgaggagc gcttccatcg tcgcccgatc cacggcgagc ccttcggtgt
     3121 cgatggcatc tgcagtcggc aggaatccga tcgcagtctc ctccgcgtcg gcatcgccgt
     3181 cgcaacgctc gagcgcccag cgcagcacgc gcaggttctc gccgaagccc ggccacagga
     3241 agttgccgtc ctcgtccttg cggaaccagt tgatatggaa aatccgcggc ggatgcttgg
     3301 cgcgccgcgc gaagctcagc caataggtcc agtaatcggc gaagttgtag ccgcagaaag
     3361 gcttcatcgc catcgggtcg cggcgcacgg ttccgacctt gccggtcgcc gccgccgtcg
     3421 tttcggaggc catgccggcg ccgaccagca ctccgtgttc ccagctcttt gcctcgtaga
     3481 tgagcggcgc cacttccgcg cgtcgaccgc cgaagaaaat cgccgaaatc ggcacgccct
     3541 gggcggcttc cgcctgcggc gaccagctcg gattctgttt cgccgagacg gtgaagcgtg
     3601 cgttcggatg ggctgcggga ccgttggccg gatcgtacgg ccggccctgc cagtcggtca
     3661 ccggctcgcc ggtcttcttg ccctcccacc agggttcctt gtccgcagtg acggcgacgt
     3721 tggtaaagat cgtgtccttg cggatcgtct cgtaggcgtt tttgttggtc ttctcgttgg
     3781 tgccgggcac gacgccgaaa tatccggcct ccggattgat cgcccacaac cgcccgtcct
     3841 cgccgacgtg cagccaggcg atgtcgtcgc ccacggtcca gatcttccag ccggggtagg
     3901 ccgcgggcgg aatgagcatg gccagattgg tttttccgca ggccgaagga aaggcggccg
     3961 cgacgtagcg cacctctccc tgcggatttt cgattccgac gatgagcatg tgctccgcga
     4021 gccagccctc gtcgcgcgcc tgccagctcg cgatccgcag tgcgtggcat ttcttgccga
     4081 gcagcgcatt gccgccgtaa ccggaaccga tgctcttgat gagacgctct tccgggaaat
     4141 gcaggatgaa acgacgatcg ggcgacagat cgccggtcga gtgcaggccg cgcacgaacg
     4201 gatcgccccg ctcgatgcgc gcgagcgccg ctgcgcccat gcgcgtcatg atctgcatgt
     4261 tggcgacgac ataggggctg tcggtgatct cgaccccgag gcgcgcgtgg ggcgagtcga
     4321 tcggccccat gcaatagggg atgacgtaca gcgttctgcc tttcatcgcg ccgtcgaaga
     4381 gcggatcgac cttggcgtgc gcctcgccgg gcgccatcca atggttgttg ggcccggcgt
     4441 cggcctcgtt tttgcagcag atgaaggtaa gatgctcgac gcgcgccaca tcggaggggt
     4501 tcgaccgatg caggtaacaa ccggggaagc tccgctgatt caactcgagc agagtgccgc
     4561 tcgcaagcat cccggcaacg agtcgccgat attcgtcggc gctgccgtcg caccaggcaa
     4621 ccttgtcggg tttggtaagg cgcgctactt cctcgaccca atcggccaag gctcggttcg
     4681 tggtcgtcat ttttttcggc tcctccggac aatcggcacg aaggcgcccg gaagagacgc
     4741 tttgcgccaa aaggggaatt atactgcgcc gcctcgttcg agtggccgat gagccttgcg
     4801 gaagaaagtg tcgcgctgct cggctcgggt ggtcttcttt gtgatcgcgt accgggattc
     4861 gtaccgcgtg agggccagcg tgcgctggcg ggggcgattg ccgcggcgat cgaggcaggc
     4921 gagcatctgc tcgccgaggc gggaaccggc atcggcaaga ccttcgcgta tctggttccg
     4981 atcctgctcg gcgggcggcg ggccctgatc tcgacggcga cacgcgctct gcaggatcag
     5041 ttgttcgagc gcgatctccc gctcgtcgcc cgcgctctcg gtcgcccgct cgacgtggtg
     5101 gtgctcaagg gacggcgtaa ttatctgtgt cgcgaacggt ggttgcgtct cgcgcaggat
     5161 tgggtcggag tcgcggcggg aggaatcgaa acggagttgc tcgcggcctg ggcggcacag
     5221 acgaagaccg gcgatctggc cgagcttccg cgcgagggta accccgccgt gatcgccgga
     5281 ctgctcacgg tcggcgccga ggcctgcctc ggcaaggcct gccccgagtt ctcccgctgc
     5341 cacgtctacg ccgcgcgtcg ccgcgcgctg gaagcggacg tggtcattgt caatcacaat
     5401 cttctcgttg cggacatcgc actcaaggcg gagggtggcg gcgatcttct tggtggcgcc
     5461 gatgtcgtcg tcgtggacga agcgcatgcg ctgcccgaag tcgcgcgtct gaacctcggc
     5521 gaaaccctgt ctcgcg
//
LOCUS       DASUJG010000178         2504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_542570, whole genome shotgun sequence.
ACCESSION   DASUJG010000178 DASUJG010000000
VERSION     DASUJG010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542570"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VFX38_03680"
     CDS             complement(<1..523)
                     /locus_tag="VFX38_03680"
                     /inference="COORDINATES: protein motif:HMM:NF015575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEX5313995.1"
                     /translation="MFFVSDGTGVTVETLGHSLLSQFETLRFRHYIIPFVTTEEKARE
                     AVARINEQARTSGIVPVVFFSMVDERLRRILEDARALTLDFFSAFLGRLERELNITSN
                     HTLGRAHGMHDAAGYLRRIEAVNYSLATDDGLNTQRYDHAQIILVGLSRVGKTPTSLY
                     LALQCGIFAANYPL"
     gene            complement(555..1292)
                     /locus_tag="VFX38_03685"
     CDS             complement(555..1292)
                     /locus_tag="VFX38_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005420223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5313996.1"
                     /translation="MSHEKLILVLKARAELTPRVLRLSFERADGDAFAYLPGQFLTLH
                     LPWQEMVLRRSYSIASIPDGAASRCIEIAATRIEGGRASGVLEALEEGERIEATGPFG
                     RFVLRDDPPSRYLLVATGTGVSPYRAMLPQLRERLRIPGFSAILMLGVRSRPELLYGG
                     DFSAFAETVENFRFLPSFSRERPEEIPAAHHGYVQERLSATSLDSARDIVYLCGNPDM
                     IDDAVAHLKLHGFSNPHIRREKYVSSN"
     gene            1284..1976
                     /locus_tag="VFX38_03690"
     CDS             1284..1976
                     /locus_tag="VFX38_03690"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5313997.1"
                     /translation="MTHGRRATISAPGQGKPFLHTVGKIRGKRAKRAAPWLIGAALTA
                     VAVAWVDRPLARAIAAVLPPRSLQPNVPDVLAPFVGAVTLASLGWFFVARRRARPRLA
                     AAALCTALAGPLAYGLKELLKWFFGRITVSWYLIHPQAAGFHWLHGQGFFFGFPSGHM
                     LVATALVTAVASFYPRLWPWGRAALAALAIALLLASYHFLGDLIAGCLLGWLLTRLLI
                     AAVARSGAVRVP"
     gene            complement(1921..>2504)
                     /locus_tag="VFX38_03695"
     CDS             complement(1921..>2504)
                     /locus_tag="VFX38_03695"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEX5313998.1"
                     /translation="PPAGEKLYPGRYETGLVALRSFDGEEIFSEPPSARDIAWARREI
                     YEPYHRELDALLARHAEEGRTILLDLHSVTPEANRISGPLDADIFLGDRDGKSCASWL
                     TDTVAERYRKAGFRVRRNDPYKGGWITERGGRLAGVEALQIEMNWAVYLDPARPAEGL
                     HSERFDAARETLQTVFTELAPLLSARLRQSAGA"
BASE COUNT          417 a          800 c          840 g          447 t
ORIGIN      
        1 tgagcgggta gttggccgcg aagatgccgc actgcaacgc gagatagagc gaagtcggag
       61 tcttgccgac ccgcgaaagc ccgacgagaa tgatctgggc gtggtcgtag cgctgcgtat
      121 tgagcccgtc gtcggtcgcg agactgtaat tgaccgcctc gatgcgccgc aggtagccgg
      181 cggcgtcgtg catgccgtgc gcgcgcccga gcgtatgatt ggaggtgatg ttgagttcgc
      241 gctcgagccg tccgagaaag gcggagaaga aatcaagggt cagggcccgc gcgtcttcga
      301 gaatccggcg caggcgctcg tccaccatgc tgaagaacac gaccggcacg atccccgagg
      361 tgcgcgcctg ttcgttgatg cgcgcgacgg cttcccgcgc cttctcttcg gtcgtgacga
      421 agggaatgat gtagtggcga aagcgcaggg tttcgaactg cgaaagaagg ctgtgcccca
      481 aggtttccac ggtaacaccg gtgccgtcgg aaacgaaaaa cacggcgcgt gcagactccc
      541 cgggcgcggc gtcgtcagtt cgaggataca tatttctccc tgcggatgtg ggggttggaa
      601 aaaccgtgga gcttgaggtg cgccaccgca tcgtcgatca tgtcgggatt cccgcaaaga
      661 taaacgatgt cgcgcgcgga atcgaggctc gtcgccgaca ggcgctcctg cacgtacccg
      721 tgatgagccg cgggaatctc ttcgggacgc tcccggctga acgagggcag aaagcggaag
      781 ttctcgacag tctcggcgaa ggcggaaaaa tcgccgccgt agagaagttc cggacggctg
      841 cgcacaccga gcatgagaat cgcggaaaat ccggggatgc gcagtcgctc gcgcagttgc
      901 ggcagcatcg cgcggtacgg tgaaacgccg gttccggtgg cgacgagaag gtaacgcgaa
      961 ggcggatcgt cgcgcagcac gaagcggccg aagggccccg tcgcctcgat gcgttccccc
     1021 tcttcgagcg cttcgagcac gccgctcgca cgcccgccct cgatgcgcgt cgccgcaatc
     1081 tcgatgcaac ggctcgcggc gccgtccgga atgctcgcga tggaatagct cctgcgcagc
     1141 accatctcct gccacggaag atgcagggtg agaaactgcc cgggaagata ggcaaaggcg
     1201 tcgccgtcgg cgcgctcgaa ggagagccgc agcacccgcg gcgtgagttc cgcgcgcgcc
     1261 ttgagaacga ggatgagttt ttcgtgactc acggcagacg tgccacaatt tccgcaccgg
     1321 gacaaggcaa accatttttg cacacggtgg ggaaaatacg cggcaagcgg gcgaaacgcg
     1381 cggcgccgtg gctcatcggc gccgccttga cggccgtcgc cgtcgcctgg gtggaccggc
     1441 cgctggcgcg cgcgatcgcc gccgtcctgc cgccgcggag cctgcagccg aacgtccccg
     1501 acgttctcgc tccgttcgtc ggcgcggtga cgctcgcaag tctcggatgg ttcttcgtcg
     1561 cgcgccgccg cgcccgcccg cgtctcgccg ccgcggcgct ttgcacggcc ctcgccggtc
     1621 cactcgccta cggcctgaag gaactcctga agtggttctt cgggcgcatc accgtttcct
     1681 ggtacctgat tcacccccaa gctgccggct ttcactggct gcacgggcag ggatttttct
     1741 tcggctttcc ctcgggtcac atgctggtcg cgacggcgct ggtgacggcg gtcgcctcgt
     1801 tttatccacg cttgtggccc tggggccggg cggctcttgc ggcgctcgcg atcgcactcc
     1861 tgctcgcctc gtatcacttc ctcggggatc tgatcgcggg ctgtcttctc ggctggttgc
     1921 ttacgcgcct gctgattgcc gcagtcgcgc gctcaggagc ggtgcgagtt ccgtaaagac
     1981 ggtttgcagc gtctcgcggg cggcgtcaaa gcgttcgctg tgcagaccct cggcgggccg
     2041 cgcgggatcg agatacaccg cccagttcat ttcgatctgc agggcttcca ctcccgcgag
     2101 ccggccgccg cgctcggtaa tccagccgcc cttgtaggga tcgttgcggc gcacgcgaaa
     2161 ccccgccttg cggtatcgtt ccgcgacggt atccgtgagc caggaagcac aactttttcc
     2221 gtcccgatcc ccgagaaata tatccgcatc gagggggccg ctgatccggt tcgcctccgg
     2281 ggttacgctg tgcagatcga gcagaatcgt ccgtccttct tcggcgtggc gcgcgaggag
     2341 cgcatcgagt tcccgatgat agggctcgta gatctcgcgc cgcgcccagg cgatgtcgcg
     2401 ggcggagggc ggctcggaaa agatctcctc gccgtcgaag cttcgcagtg ccacgaggcc
     2461 ggtttcgtag cgaccgggat aaagcttctc gccggccggc gggc
//
LOCUS       DASUJG010000179         3563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1644163, whole genome shotgun sequence.
ACCESSION   DASUJG010000179 DASUJG010000000
VERSION     DASUJG010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1644163"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..178
                     /locus_tag="VFX38_03700"
     CDS             26..178
                     /locus_tag="VFX38_03700"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HEX5313999.1"
                     /translation="MVLFPSYMRHEVSPFWGRDERITVAFNAWLREAGQAVDEPNLRR
                     REEQPA"
     gene            180..1292
                     /locus_tag="VFX38_03705"
     CDS             180..1292
                     /locus_tag="VFX38_03705"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX5314000.1"
                     /translation="MSESGELLRSAREDLARGWSVRAGTRLRQAAAAGEPDALAALLR
                     ALLLHQIPGGASEARARLEAGTALAPELLWLRATLRYVALGGPADPRGAIADLSAAAS
                     GGLAEAAMELALLCHEAGDIAGSRSWLSQVAASHALARDLLLLLPPAADAIPGSSPIP
                     GDWPEQRKPATREQLSADPAIVRFREAFHPLECAWLRGISRPRLAPSLVVDPQTGRPR
                     PDATRTSETMCFDPASPGVYARRMAARMARLAGRDPACGEPLSILRYRPGQEYKPHYD
                     WMGAAGLAGDGFRGAGERAMTALGYLNVPDQGGATLFTRLDIRVEPGLGDVLVFSNLD
                     STGRPEMRSQHAGEPVAAGEKWLASLWIRERAVPLG"
     gene            1297..2595
                     /locus_tag="VFX38_03710"
     CDS             1297..2595
                     /locus_tag="VFX38_03710"
                     /inference="COORDINATES: protein motif:HMM:NF016974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEX5314001.1"
                     /translation="MVEASNAADEALAYARELAGRGRTVQARELCEQLLAVHPEHPAT
                     LNFLGELALDGNQPKEAARLFHRARELAPDDLAALLNLALAEMASGRMEAALARFEET
                     LARDPRHALALIHKGRALELAGRPREAVETWYLALEEANRQELWNRPETTPPALRALL
                     ARATVAVRRARERFLRERLHELRERHGDAALARVWKCLDLYLGKRPLEFANPEQRPSF
                     LVFPDIPSRNFFRREEFPELRNIEAKTALIRDELAAVMNVGEGFRPFIDMPPGPAAEH
                     WRRLNRSADWNAFFFWRDGERNEENCRRCPETAASLDALDLNRVPGHSPESIFSVLTP
                     GTHIPPHHGVTNTRLVCHLPLVIPENCAIRVGGEERGWTEGECLIFDDTFRHEAWNRS
                     DRIRVVLIFDLWNPYLTPVEREAMSILVPAAGAFNRQMVR"
     gene            complement(2653..3336)
                     /locus_tag="VFX38_03715"
     CDS             complement(2653..3336)
                     /locus_tag="VFX38_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314002.1"
                     /translation="MNVEAVYQHFYRVGRLAAEELSDGGGPFGFDITTALEVDWLIET
                     YGCDALFETGCNMGDTTAYLCRRYPALPLFSCDVKPQYVEATRRRVSGRSNAFVEELD
                     SPELIARHAGDFRFPFFYLDAHWYEDWPLERELARIERGVVMVDDFDIGHPRFGFDEY
                     QGQRCGPELLARFRAKISRYYTNNPDALYEFPCLQTGRRGGKAYFLVGEERDYLRHCR
                     YFLRREFAP"
BASE COUNT          593 a         1222 c         1168 g          580 t
ORIGIN      
        1 gccgctgcgg ctgcgtcccg gtgagctggt gctgtttccc tcctacatgc gccacgaagt
       61 ctcgcccttc tggggccgcg acgaacgcat caccgtggcc ttcaacgcct ggctgcgcga
      121 ggcgggccag gcggtggacg agccgaatct gcgccgccgc gaggagcagc cggcctgaga
      181 tgagcgagag cggcgaactt ctgcgctccg cccgcgaaga cctcgcccgc ggctggagcg
      241 tgcgtgcggg cacgcgcctg cgtcaggccg cggcggcagg tgagccggat gcgcttgcgg
      301 ccctgctgcg ggcgcttctt ctgcaccaga ttccgggcgg ggcgagcgaa gcccgcgctc
      361 ggctggaagc cggtactgcc cttgcacccg aattgctgtg gctccgcgcc acgctgcgtt
      421 atgtcgcgct cggcgggccc gccgatccgc ggggcgcaat cgccgatctg agcgcggcgg
      481 cctccggcgg tctcgccgag gcggccatgg aactcgctct gctctgtcac gaagccggcg
      541 acatcgccgg cagccggagt tggctgtcgc aagtcgcagc gagccatgcg ctcgcccgcg
      601 atctgctgct gcttctgcca ccggcggcag atgcgattcc cggatcgtcg ccgattccgg
      661 gcgactggcc ggaacaacgc aaaccggcga cgcgcgagca gttgagtgcc gatccggcga
      721 tcgtgcgttt ccgcgaggct ttccatccgc tggaatgtgc ctggctgcgg ggcatctccc
      781 gcccacggct cgcgccctcg ctggtggtgg atccgcagac cggtcggccg cgccccgatg
      841 cgacgcgcac gagcgaaacc atgtgtttcg acccggcttc cccgggcgtg tacgcgcggc
      901 gcatggcggc gcgaatggcg aggctcgccg gccgcgatcc cgcctgcggc gagccgcttt
      961 cgattctgcg ctaccgcccc ggacaggaat acaaaccgca ttacgactgg atgggtgcgg
     1021 cggggctcgc gggtgacggc tttcgcggcg ccggcgaacg cgcgatgacg gcgctcggct
     1081 atctcaacgt tcccgatcag ggaggcgcga cgctcttcac gcgcctcgac atccgcgtcg
     1141 aaccggggct cggagacgtg ctggtctttt cgaatctcga ttcgaccgga cggccggaaa
     1201 tgcgcagtca acatgccggc gagccggtcg ctgcgggaga gaaatggctc gcctcgctgt
     1261 ggatccgcga gcgggcggtc ccgctgggat aggcgagtgg tggaagcatc caatgccgcc
     1321 gatgaggcgc ttgcctatgc ccgcgaactc gcgggtcgcg gccgcacggt ccaggcccgg
     1381 gagctttgcg agcaactcct cgccgtgcat ccggagcatc cggcgacgtt gaattttctc
     1441 ggcgaacttg cgctcgatgg gaatcagccg aaggaagcgg cgcgcctttt ccaccgcgca
     1501 cgcgaactcg ctccggacga ccttgccgct ctcctcaacc tcgcccttgc cgagatggcg
     1561 agcggcagaa tggaggcggc tctcgcccgc ttcgaagaaa ccctcgcgcg tgatccccgg
     1621 cacgctctcg cgctgattca caagggccgg gcgctggaac tcgccggccg gccgcgcgag
     1681 gcggtcgaga cctggtacct cgcgctcgaa gaggcaaacc ggcaggaact ctggaaccgg
     1741 cccgagacga ctccacccgc actgcgagcg ctgctcgcgc gcgccaccgt cgcagtgcgc
     1801 agggcgcgcg aacgattcct gcgcgaacgc ctgcacgaac ttcgcgagcg ccatggcgac
     1861 gccgcgctcg cgcgcgtctg gaaatgcctc gatctctatc tcggcaaacg cccactggaa
     1921 ttcgcgaacc ccgagcagcg cccgagtttt ctcgtctttc cggacattcc ctcgcgcaat
     1981 ttcttccggc gcgaggagtt tccggaactt cgaaacatcg aggcgaagac cgccctcatc
     2041 cgcgatgaac tcgcagccgt gatgaacgtc ggcgaaggct tccgcccctt catcgacatg
     2101 ccgcccggtc ccgcggccga gcactggcgg cggctcaacc gttcggcaga ctggaacgcg
     2161 tttttcttct ggcgcgacgg ggagcgcaac gaggaaaact gccggcgctg tcccgagacg
     2221 gcggcgtcac tggatgcgct cgatctcaac cgcgtccccg ggcattcgcc ggaatcgatc
     2281 ttttccgtgc tgacccccgg cacccacatt ccgccccatc acggcgtaac caacacgcgg
     2341 ctcgtctgtc atctgccgct cgtcatcccg gagaactgcg cgatccgcgt cggcggcgaa
     2401 gagcggggct ggaccgaggg cgagtgtctg atcttcgacg acaccttccg tcacgaggcg
     2461 tggaaccgca gcgatcgcat ccgcgtggtc ctgatcttcg atctttggaa tccatatctc
     2521 acgccggtgg aacgcgaggc gatgagcatc ctcgttcccg cggccggcgc cttcaaccgc
     2581 cagatggtca ggtagcagcc aagcgggagc gagttcgcga atgcgccgcc ggatcgcggg
     2641 catggcccgc tcctacgggg caaactcgcg gcgcagaaaa taacggcaat ggcggaggta
     2701 atcgcgctcc tcgccgacga gaaaatacgc ctttccgccg cgccgccccg tctgcagaca
     2761 ggggaattcg tacaaggcat cggggttgtt ggtgtagtat cgcgaaatct tggcgcgaaa
     2821 acgcgcaagc agttccggcc cgcagcgctg cccctggtac tcgtcgaagc cgaagcgcgg
     2881 gtggccgatg tcgaagtcgt ccaccatcac gaccccgcgc tcgatgcggg cgagttcccg
     2941 ctccagcggc cagtcctcat accaatgggc atcgaggtag aaaaacggaa agcggaaatc
     3001 gcccgcgtgg cgcgcgatca gctcgggaga atccagctcc tcgacaaacg cattgctgcg
     3061 cccggaaacg cgccgccgcg ttgcttcgac atactgcggc ttcacgtcac aactgaagag
     3121 cggaagcgcg ggataacgcc ggcacaaata ggccgtggtg tcgcccatgt tgcagccggt
     3181 ctcgaagagg gcgtcgcagc cgtacgtttc gatgagccaa tccacttcga gcgccgtcgt
     3241 tatgtcgaag ccgaaggggc cgccgccatc ggaaagctcc tcggccgcga gccgcccgac
     3301 gcgatagaaa tgctggtaca cggcctcgac gttcatcgcg gcattataaa tgcccgcaac
     3361 gcggctttgc gggttgtccg ggcctgcttt gcctgcgtag gagcgggcca tgcccgcgaa
     3421 caggcatcat agacgccatg gccaagctgc caacccaagg caaccccggc aatcgggcgt
     3481 tgcgaaaagg ccgcgtatcc ctgcccggtc actcttatct gatcacgtcc gtcacgcgtg
     3541 ggagggaacc gttcttcggc gac
//
LOCUS       DASUJG010000180         4348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_177689, whole genome shotgun sequence.
ACCESSION   DASUJG010000180 DASUJG010000000
VERSION     DASUJG010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177689"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VFX38_03720"
     CDS             <1..380
                     /locus_tag="VFX38_03720"
                     /inference="COORDINATES: protein motif:HMM:NF027463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HEX5314003.1"
                     /translation="TFMLRRQAGITLIGGILVLIIAGILVLAAVKIVPLYFEAAKLGS
                     VMKSVGESARGDPLGDLRGRLAEQLTINSIDDVPLNQFRFSSQGDQLVISLDHDLKAT
                     FIGNLGFVVHYRHSVTVSREGDD"
     gene            373..1047
                     /gene="rnc"
                     /locus_tag="VFX38_03725"
     CDS             373..1047
                     /gene="rnc"
                     /locus_tag="VFX38_03725"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017118163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX5314004.1"
                     /translation="MTDEAERFAERIGYRFADPGLIALALAHRSSPGASNERLEFLGD
                     AVINLAIAEVLYRRLPELDEGALTRLRASLVNGEMLAAVARELDLGAFLRLGAGEQKS
                     GGAMRASILADAFEAVLGAVFVDGGYAAARALLRRLFDARLRALPDAQAVKDAKTRLQ
                     ELLQARGLGLPDYALAAAEGPEHARRFVAECRVRELDLRASGAGSSRRDAEQAAAGEM
                     LARLDS"
     gene            1044..1943
                     /gene="era"
                     /locus_tag="VFX38_03730"
     CDS             1044..1943
                     /gene="era"
                     /locus_tag="VFX38_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005991962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX5314005.1"
                     /translation="MSDAAPIRAGFVAVVGRPNVGKSTLVNALVGRHVAIVSRRPQTT
                     RRRILGVANRPGAQLVLVDSPGLHGAGGRPLNRALNASARRAAADADAVLLVAEAAAW
                     DEDDARALAVVRDGGMPVVLALNKIDLLRPRSALLPLLEAASARHDFAAVVPVAAARG
                     ENIERLASVLTPLLPPSPLLFPPGEITDLGPAERAAEFVREALIERLGQELPYATYVT
                     VGRYAQENSCLDIEATIWVARESQKGIVIGAGGRMAKAIGTAARRRLEAEVGGKVMLR
                     LWVRVRAGWDRDPGALAELGIGL"
     gene            1940..2662
                     /gene="recO"
                     /locus_tag="VFX38_03735"
     CDS             1940..2662
                     /gene="recO"
                     /locus_tag="VFX38_03735"
                     /inference="COORDINATES: protein motif:HMM:NF014607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX5314006.1"
                     /translation="MSERREARLVPAWVLHSYPYRETSLIVEALSRDHGRIGLVARGA
                     RRNARGSLRPAPFAPLLLSFRQHGELATLSGCEADGAPYRFGGDAFLAACYLNELLLR
                     LLPRDDPAPEVYAIYTEALAALAPVPAPAVRRFEGRLLRILGWAPPWGRDASGAVLDS
                     ARRYRYDPRRGPVPVAREEEGFGADMFEAIAAERFEEPEVLAAAAKIFREIIAYHLGG
                     KRLKTLEVARSLSRGNERSSAA"
     gene            2659..3378
                     /gene="pdxJ"
                     /locus_tag="VFX38_03740"
     CDS             2659..3378
                     /gene="pdxJ"
                     /locus_tag="VFX38_03740"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017640154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEX5314007.1"
                     /translation="MSAIRLGVNVDHVATLRQARGTPYPDPVRAALVAEEAGADGITV
                     HLREDRRHIQERDVRLLRELLATKLNLEMAVVAPKIAFARELRPQDVCLVPERREELT
                     TEGGLDVAAEPARYRDAVAELAAADIRVSLFLDPEARQIEAAAAVGAAVVELHTGAYA
                     NAEAESAVKAELERLRDAAERALDLGLIVNAGHGLHYRNVREICRLPGLDELNIGHAI
                     VARALFVGMAAAVAEMKALLR"
     gene            3375..3761
                     /gene="acpS"
                     /locus_tag="VFX38_03745"
     CDS             3375..3761
                     /gene="acpS"
                     /locus_tag="VFX38_03745"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX5314008.1"
                     /translation="MKIYGVGTDVLELARMHRLWERWGERAERHILTARESERFARAR
                     DRGRFLGMHFAAKEAVTKALGTGFCPRMTLHDVEVIPDRLGRPEVYFSDKGAAFVAER
                     GGGETHISLTDDAGIVFAVCVMLVKD"
     gene            complement(3873..>4348)
                     /locus_tag="VFX38_03750"
     CDS             complement(3873..>4348)
                     /locus_tag="VFX38_03750"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX5314009.1"
                     /translation="PLGIIQRGPVAYLVATAFDYADVRYFALHRIRKAEVLMDTAKRP
                     AGFSLDDHLATGAAHFTTDTPVRSIRLRAQVSAELARILEETALSSDQKLSQAKSTDD
                     ILVTASLPDTWQLRWWILSQGSSLEVLAPKALRQYVADEVAELSKTYRKTNTRKS"
BASE COUNT          658 a         1482 c         1470 g          738 t
ORIGIN      
        1 gaacgttcat gctcaggcgg caagcgggaa tcaccctgat cggcggcatc ctggtcctga
       61 tcatcgcggg tattctggtg ctcgcggcgg tcaagatcgt gccgctgtac ttcgaggcgg
      121 cgaagctcgg ctcggtcatg aagtcggtcg gcgagagcgc ccgcggcgat cctctcggcg
      181 acctgcgcgg gagactcgcc gaacagttga cgatcaacag tatcgacgac gtgccgctca
      241 atcaattccg gttttcctcg caaggggatc aattggtcat ttcgctcgat catgacctca
      301 aggcgacctt catcggcaat ctcggcttcg tcgtgcatta ccgtcattcc gtcacggttt
      361 cccgcgaagg agatgactga cgaggcggag cgcttcgccg agcgcatcgg ataccgcttc
      421 gccgatcccg gactgattgc gctcgcgctc gcgcaccgca gcagtcccgg cgcgagcaac
      481 gaacggctcg aatttctcgg cgacgccgtc atcaatctcg cgatcgccga ggtgctctac
      541 cggcgcctgc cggaactcga cgaaggagcg ctcacgcgtc tgcgtgcgag cctcgtgaac
      601 ggcgaaatgc tcgcggcggt cgcaagggaa ctcgatctcg gggcatttct gcgcctcgga
      661 gccggcgagc agaaaagcgg cggggcgatg cgggcgtcga ttctcgcgga cgctttcgag
      721 gccgtgctcg gcgcggtgtt cgtggatggc ggctacgctg ccgcgcgcgc gctcttgagg
      781 cggctcttcg atgcccgtct gcgcgcgttg ccggatgcgc aggccgtcaa ggacgccaag
      841 acccggctgc aggaactgct gcaggcgcgc ggactcggcc tgccggacta tgcgctcgcc
      901 gcggccgaag ggcccgaaca tgcgcgccgc ttcgtcgccg aatgtcgcgt gagggaactc
      961 gatcttcgcg cgagcggcgc gggctccagc cgccgcgatg ccgagcaggc ggcggccggc
     1021 gaaatgctcg cgcgtctcga ttcgtgagcg acgccgcgcc gatccgcgcc ggctttgtcg
     1081 ccgtcgtcgg ccgccccaac gtcgggaaat ccacgctcgt caacgcgctc gtcggcaggc
     1141 atgtggcgat cgtttcgcgg cgtccgcaga ccacgcgccg gcgcattctc ggcgtcgcca
     1201 accggcccgg cgcccaactc gttctcgtgg acagccccgg cctgcacggc gccggcggcc
     1261 gcccgctcaa ccgcgccttg aatgcgagtg cgaggcgcgc ggcggcggat gccgacgcgg
     1321 tgctgctggt cgccgaagcc gcggcctggg acgaagacga tgcgcgcgcc ctcgccgtcg
     1381 ttcgcgatgg cgggatgccg gtggtgctcg ctctcaacaa gatcgatctt ctccgtccgc
     1441 gttcggcgct gctgcctctg ctcgaagccg caagcgctcg gcacgacttc gccgcggtgg
     1501 ttcccgtcgc cgccgcgcgc ggcgaaaaca tcgagcgatt ggcgagcgtg ctcacgccgc
     1561 ttctgccgcc ttcgccgctg ctgtttccgc cgggcgaaat caccgatctc ggcccggcgg
     1621 aacgcgccgc cgaattcgtg cgcgaggccc tgatcgaacg gctcggccag gagcttccgt
     1681 acgcgactta tgtcacggtc ggacgctacg cgcaggaaaa ctcctgcctc gacatcgagg
     1741 cgacgatctg ggtggcgcgc gaaagccaga agggcattgt catcggcgcc ggcggacgca
     1801 tggccaaggc catcggaacg gctgcgcgcc gccggctcga agccgaagtc ggcgggaagg
     1861 tgatgttgcg gctgtgggtg cgcgtgcgcg ccggctggga tcgggatccc ggcgcgctcg
     1921 ccgaactcgg aatcggcttg tgagcgaacg gcgcgaggcg cggctcgttc ccgcctgggt
     1981 attgcacagc tatccgtatc gcgaaaccag cctgatcgtc gaggccctga gtcgcgatca
     2041 cggacgcatc ggtctcgtcg ctcgcggcgc ccgcagaaac gcgcgcggca gcctgcgccc
     2101 ggcgcccttc gctccgctgc tgctttcgtt tcgccagcac ggagaactcg cgaccctcag
     2161 cggctgcgaa gcggacggtg ctccgtaccg cttcggcggc gatgccttcc tcgcggcctg
     2221 ctatctcaac gaattgctgt tgcgtctgct gccgcgcgac gatcccgctc ccgaggtgta
     2281 cgccatttac accgaagctc tcgccgcgct cgcgcccgta cccgcgcccg ccgtgcgtcg
     2341 cttcgaggga cgcctcctgc gcattctcgg ctgggcgccg ccctgggggc gcgatgcctc
     2401 cggcgccgtg ctcgattccg cgcggcgcta ccgttacgat ccgcgccgcg gtccggttcc
     2461 cgtcgcccgc gaagaggagg gattcggcgc cgacatgttc gaggcgatcg ccgccgagcg
     2521 ctttgaggaa ccggaggtgc tcgcggcggc cgcgaagatt ttccgcgaga tcatcgccta
     2581 tcatttgggc gggaagcgtt tgaagaccct cgaggtcgcc cgctcccttt cccgcggcaa
     2641 cgaaaggagt tccgcagcat gagcgcgatc cgactcggcg tgaacgtcga tcatgtcgcg
     2701 acgctgcggc aggcgcgcgg gacgccttat cccgatccgg tgcgcgcggc tctggtcgcg
     2761 gaagaggcgg gcgccgacgg catcaccgtg catctgcgcg aggatcgccg gcacattcag
     2821 gagcgcgacg ttcgcctgct gcgggaactc ctcgcgacca agctcaacct cgaaatggcg
     2881 gtcgtcgcgc cgaagatcgc cttcgcccgc gagctgcgcc cgcaggatgt ctgcctcgtt
     2941 cccgaacgcc gcgaggaact cacgaccgaa ggcggactcg acgttgccgc cgagcccgcg
     3001 cgctatcgcg acgccgttgc cgaacttgcg gcggcggaca ttcgcgtttc gctctttctc
     3061 gatccggaag cgcggcagat cgaagccgcc gccgccgtcg gcgccgcggt cgtcgagctg
     3121 cacaccggcg cctacgcgaa tgcggaggcg gagtcggcgg tgaaggcgga actcgaacgt
     3181 ctgcgggatg cggcggagcg ggcgctcgat ctcggcttga tcgtcaatgc cggtcacggg
     3241 cttcattacc gcaacgtgcg cgaaatctgc cgcttgccgg gtctcgacga actcaacatc
     3301 ggccatgcga tcgtcgcccg cgcgctgttc gtcggcatgg cggcggcggt cgcggaaatg
     3361 aaggcgctgc tgcgatgaag atctacggcg tcggcaccga cgtcctcgaa ctcgcgcgca
     3421 tgcatcgtct gtgggaacgc tggggcgagc gcgccgagcg ccacatcctc acggcgcgcg
     3481 agagcgagcg cttcgcccgc gcacgcgatc gcggccgctt tctcggcatg catttcgcgg
     3541 ccaaggaagc cgtgaccaag gcgctcggca ccggcttctg cccgcgcatg acgctgcacg
     3601 atgtggaagt cattcccgat cgcctcgggc gacccgaagt gtatttctcg gacaagggcg
     3661 cggccttcgt cgccgaacgc ggcggcggcg aaacccacat cagcctcacc gacgatgcgg
     3721 gcatcgtctt cgccgtctgc gtcatgctcg tcaaagattg aacgctcgga gcagcgcatc
     3781 gccgtcgcaa tagacttccg taccggccgc atcggctcgc ttcagccagc cgcgtcgcga
     3841 taataaacgt cgccgggtcg gcctcgatcg gctcaggact tgcgtgtgtt ggtcttgcgg
     3901 taggttttgc tcagttcggc aacttcgtcc gcgacgtatt ggcgcagcgc cttcggggca
     3961 agcacctcca gactcgagcc ctgagacagg atccaccagc gcagttgcca ggtgtcgggc
     4021 agcgaggcgg taacgagaat gtcgtccgta ctcttggcct gactcagctt ctgatcggac
     4081 gatagcgcgg tctcctcgag aatgcgcgca agctcggcgc tgacttgcgc ccgcagccgg
     4141 atgctgcgca cgggcgtgtc ggtcgtaaag tgcgcggcgc cagtggccaa atgatcgtcg
     4201 agcgagaagc ccgccggtcg cttggccgtg tccatcagca cctccgcctt gcggatgcgg
     4261 tgcagggcga agtagcgaac gtctgcgtag tcgaaagccg tcgccaccag gtacgcaacc
     4321 ggcccgcgct ggatgattcc cagcggat
//
LOCUS       DASUJG010000181         3409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_177883, whole genome shotgun sequence.
ACCESSION   DASUJG010000181 DASUJG010000000
VERSION     DASUJG010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177883"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..383)
                     /locus_tag="VFX38_03755"
     CDS             complement(3..383)
                     /locus_tag="VFX38_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5314010.1"
                     /translation="MHHSRLAAVLIDCRGEDLDAAGDFWSRALGYAQKVGDTVDPERY
                     RVLATPDHQLRCEVQLVAHDSRVHLDIETDDIEAEVSRLQALGAREAQRIASWVVMEA
                     PTGQRFCVVRPQRADFPRGANRWD"
     gene            complement(433..1974)
                     /locus_tag="VFX38_03760"
     CDS             complement(433..1974)
                     /locus_tag="VFX38_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007420798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEX5314011.1"
                     /translation="MAKEVKIPDIGGFEGVEVIDVLVGEGDKIAKEDSLITLESDKAA
                     MDVPAPFAGTVRELKVKAGDKVSEGDVILTVEGSEEDGEDESDQRKESKENDAESAEE
                     DRAQEADEKEDKDRKNGEGGNELQDAEGEKSKGTRKRESKKPKRKDKAEEDAREEGES
                     EDDQSEEDETEKDERQSRRDAESKDRERTRSRKSENAKAGPSGEGGATPPTVDRISQV
                     TAHAGPSVRRFARELGVDLSRVKGSGNKSRISHEDVRRHVKSLLHGGAVAGGLPQIPE
                     VDFSRFGEIARKPLSRIKRISGPRLQAAWINAPHVTQHDEADITALETVRKAMNERAA
                     TDDAKLTPLAFLMRAVVLAMQALPDFNSSLDRDGENLICRKYFHIGFAVDTDEGLIVP
                     VIRDADRKSIREIAADLAALAEKARGGKLARKDIEGGCFSISSLGSIGGTAFTPIINA
                     PEVAVLGVSRAEMKPKWNGSAFEPRLMLPLSLSYDHRVIDGAQAVRFTRHLAETLETL
                     RGMVR"
     gene            complement(1991..>3409)
                     /gene="aceE"
                     /locus_tag="VFX38_03765"
     CDS             complement(1991..>3409)
                     /gene="aceE"
                     /locus_tag="VFX38_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012981764.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEX5314012.1"
                     /translation="DDLKAYRDRLNIPVSDEEIHELPFVKPGEDSLEMHYLEERREAL
                     GGSLPMRIAEAPPLEMPADKRFEVLTEGSGEREISTTMAIVRLVTSLTRDKKFGARIV
                     PIVPDEARTFGMEGLFRQIGIYSPMGQLYEPEDAEQLMHYKEDKKGQILQEGITEAGC
                     MASWMAAASAYANYGVQMVPFFFFYSMFGFQRFGDLAWAAGDIHARGFLIGGTAGRTT
                     LEGEGLQHCDGNSHIYASVFPSCVSYDPAYGYEIAVIVREGIRRMFVGQENVFYYLTT
                     MNENYAHPPLPAGAEEGIIRGMYRLKDVGEHELHVRLLGSGTILREAEAAAEILDRDW
                     QIASEVWSVTSFEELRREGLRLRRADTLHASAEAQLPWVTRCLENGKGPVIAATDYTK
                     LYADKIREFVPGRYVTLGTDGFGRSDHRRDLRRFFEVDREHIVVHALKALADEGALEY
                     RKVAEAIERYGIDPDTPPREFA"
BASE COUNT          535 a         1149 c         1026 g          699 t
ORIGIN      
        1 tctcaatccc agcggtttgc gccgcgagga aaatccgcac gttgcgggcg cacgacgcag
       61 aaacgctggc ccgtgggtgc ctccatcacc acccagcttg cgatccgctg cgcttcgcgc
      121 gcgccgagcg cctggaggcg agagacctcg gcttcgatgt cgtcggtttc gatgtcgaga
      181 tgcacgcgac tgtcgtgcgc gaccaactgt acttcgcagc ggagctgatg gtccggagtt
      241 gcgagcacgc gatagcgttc gggatcgacg gtgtcaccga ccttctgcgc gtaacccagc
      301 gcccgcgacc agaagtcgcc ggccgcgtcg agatcctcgc cccggcagtc gatcagcacc
      361 gctgcgagcc ggctgtggtg catcgttatt cgaggctccc cgcgagaatc gcgagcaagc
      421 cggctcccgc cttcatcgca ccatcccgcg gagcgtttcc agggtttcgg cgaggtggcg
      481 cgtgaagcgc acggcctgcg cgccgtcgat cacgcggtga tcgtaggaaa gcgacagcgg
      541 caacatgagc cgcggctcga aggcgctgcc gttccacttc ggcttcattt ccgcacgcga
      601 aacgccgaga acggcgacct ccggcgcgtt gatgatcggc gtgaacgccg tgccgccgat
      661 gctcccgagg ctggaaatcg aaaagcagcc gccttcgatg tccttgcgcg cgagttttcc
      721 gccgcgtgcc ttttccgcca gagccgcaag gtccgcggcg atctcccgaa tggactttcg
      781 atccgcatca cggatcaccg ggacgatcaa tccctcgtcg gtgtccaccg cgaaaccgat
      841 gtgaaagtat tttctgcaga tcagattctc cccatcgcga tcgagcgagg agttgaaatc
      901 gggaagcgcc tgcatcgcga ggacgacggc gcgcatgaga aatgcgagcg gcgtgagttt
      961 ggcatcgtcc gttgcggcgc gctcgttcat cgccttgcgc acggtctcga gcgccgtgat
     1021 gtcggcctcg tcgtgctggg ttacgtgcgg cgcgtttatc caggccgcct gcaggcgagg
     1081 gccggaaatg cgcttgatgc gcgacaaggg tttgcgcgcg atttcgccga agcgcgagaa
     1141 atccacctcg ggaatctgcg gcaatccgcc ggcgacggcg ccgccgtgaa gcagggattt
     1201 cacgtggcgg cgcacgtctt cgtggctgat ccggctcttg ttgccggagc ccttgacccg
     1261 cgacaaatcc acgccgagtt cgcgagcgaa tctgcgcacg gaagggcccg cgtgcgccgt
     1321 gacctgcgaa atccgatcta cggtcggcgg agtcgcgccg ccctcgcccg aaggtcccgc
     1381 cttcgcgttc tcacttttcc gcgaccgtgt ccgctcgcga tccttagatt ccgcatcgcg
     1441 gcgcgattgg cgctcgtctt tctccgtttc gtcctcttcc gactgatcgt cctccgattc
     1501 gccttcctcg cgcgcatctt cttccgcctt gtcctttcgc ttcggttttt tggactccct
     1561 cttccgggtt cctttgcttt tctcgccttc cgcatcttgc aattcgttcc cgccttcgcc
     1621 gttcttcctg tccttgtctt ctttctcgtc cgcttcctgg gctcgatctt cctccgccga
     1681 ctccgcgtcg ttctctttgc tttctttgcg ttgatccgat tcgtcctcgc cgtcttcctc
     1741 gctgccctcg acggtgagaa tcacgtcgcc ctccgacacc ttgtcgccgg ccttgacctt
     1801 gagttccctg acggtgcccg caaagggcgc cggcacatcc atcgcggcct tgtccgactc
     1861 cagcgtaatc agcgagtctt ccttcgcgat cttgtcgcct tcaccgacca gcacatcgat
     1921 gacctcgacg ccttcgaagc cgccgatatc gggaatcttg acctctttcg ccacgccgcc
     1981 gtccttcttc ctatgcgaat tcgcgaggcg gcgtatccgg atcgatgccg taacgctcga
     2041 tcgcctcggc caccttccga tattcgagcg ctccctcgtc ggcgagcgcc ttgagcgcgt
     2101 gcaccacgat gtgctcgcgg tccacttcga aaaaacggcg cagatcgcgc cggtgatcgc
     2161 tccgcccgaa accgtcggtg ccgagcgtga cgtatcgccc cggaacgaat tcgcggatct
     2221 tgtcggcgta aagtttggtg tagtcggtcg ccgcgatcac cggccccttt ccgttctcga
     2281 gacagcgcgt gacccaggga agctgcgcct cggcggaagc gtggagcgtg tccgcgcggc
     2341 gcagacggag cccctcgcgg cgcagttcct cgaaactcgt cacactccat acctcgctcg
     2401 cgatctgcca gtcgcgatcg agaatttccg ccgccgcctc ggcctcgcga agaatcgttc
     2461 cggagccgag aagacgcacg tgaagctcgt gctcgccgac gtccttgagc cggtacatgc
     2521 cgcggatgat gccttcctcc gctcccgccg gaagcggcgg atgggcgtag ttttcgttca
     2581 tcgtcgtgag gtagtagaaa acgttctcct gcccgacgaa catccggcgg atgccctcgc
     2641 gcacgatcac cgcgatctcg tagccgtagg cgggatcgta ggaaacgcag ctcggaaata
     2701 cgctcgcata aatgtgactg ttgccgtcgc agtgctgcag gccctcgcct tcgagcgtgg
     2761 tgcgcccggc ggtgccgccg atgagaaagc cacgcgcgtg gatgtcgccc gccgcccagg
     2821 caagatcgcc gaagcgctgg aacccgaaca tggaatagaa gaaaaagaag ggcaccatct
     2881 gcacgccgta attggcatag gcgctcgccg cggccatcca ggaggccatg cagcccgctt
     2941 cggtgattcc ttcctgcaga atctggccct tcttgtcctc cttgtagtgc atgagctgct
     3001 ccgcgtcttc gggctcgtag agttgaccca tcggcgaata aatcccgatc tggcggaaaa
     3061 gtccctccat gccgaaggtg cgcgcctcgt cgggcacgat cggcacgatg cgcgcgccga
     3121 atttcttgtc gcgcgtgagc gaggtgacga gccgcacgat cgccatggtg gtggaaatct
     3181 cgcgctctcc ggagccctcg gtcagcacct cgaagcgttt gtccgccggc atctcgagcg
     3241 gcggcgcctc ggcgatcctc atcgggagcg aaccgccgag cgcctcgcgg cgctcctcga
     3301 gatagtgcat ctcgaggctg tcctcgccgg gcttgacgaa gggaagctcg tgaatctcct
     3361 cgtcggatac cgggatgttg aggcggtcgc ggtaggcctt gagatcgtc
//
LOCUS       DASUJG010000182         9669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_911895, whole genome shotgun sequence.
ACCESSION   DASUJG010000182 DASUJG010000000
VERSION     DASUJG010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9669
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911895"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="VFX38_03770"
     CDS             <1..850
                     /locus_tag="VFX38_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX5314013.1"
                     /translation="ALEKLADGFRWLEGPVWFADLQCLLFSDIPNNRVMRWTESGGVS
                     VFREPSDFENGHTRDREGRLISCSHRARAIRRTEPDGATTVLADRHRGKRLNSPNDVV
                     VKSDGSIWFSDPPYGIQTDYEGGKQESELPANLYRLDPTTGELAVVADDFEGPNGLCF
                     SPDEKRLYVTETGLQFAENPQRHIRVFAVADDGRRLAKGRVFHKVSPGNADGIRCDED
                     GNVWSSAGDGVHCIAPDGTLLGKIKVPSIVSNLCFGGRERSRLFLCASQTLYAVYVNR
                     RGACRP"
     gene            847..1803
                     /locus_tag="VFX38_03775"
     CDS             847..1803
                     /locus_tag="VFX38_03775"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5314014.1"
                     /translation="MNDNRGPVRRVLALSRTVSDLDRAVAFYREALDFSLLGRERREQ
                     SAWAELLGLPRVRGEVARLGLGEEKLELVACEPAGRAYPPESGVADHWFQHVAIVVSD
                     MAAACERLDARPFEAISENGPQQLPANTGSVTAYKFRDPDGHPLELLHFPTGSGDPGW
                     QRKPGLFLGLDHSAITVADLNESLDFYTRLLGFTIAGRSLNSGPAQANLDHAANVHVR
                     VVALQPANAAGAPHLELLCYERPAGQAMLPDTKPDDVFADRLLLEVSDLPALVRVLEA
                     EAVSFLSPGMVRLANGERAALVRDPTGHLLLLSESAGAAQAR"
     gene            1834..3126
                     /locus_tag="VFX38_03780"
     CDS             1834..3126
                     /locus_tag="VFX38_03780"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5314015.1"
                     /translation="MTPEPRANSAGLALSRIVIVGAGFGGLACAQALRRARAKVTVVD
                     QRNFHLFQPLLYQVATAALSPAEISMPIRRILRRQANSRVELGRVAAVDRNARCVRFE
                     ADDRALAYDYLVVATGSTHAYFGHDDWARFASPLKTIEDATAIRGHILRALERAELSE
                     EESERARLLSFVVVGGGTTGAEMAGAIAELARAELAMPHRRLHGRRARVVLIEAGPRL
                     LPSFPPALAAKARRSLEGLGVEVLTQHAVAHCDDRGVIAAGRRIEAATVIWAAGVAAS
                     PAARWLDADADKAGRVRVAADLSLPGDPAVFVIGDSAAVFNRDGSPVPGMAPPAKQEG
                     AYVGRLLARRLAGKPPPAPFRYRNLGDLATIGRKSAILDLGWLRLSGLPGWLVWGGVH
                     IFFLIGFRNRFVVAFSWLWSYFTRDYGARLITGGERKP"
     gene            3225..6437
                     /locus_tag="VFX38_03785"
     CDS             3225..6437
                     /locus_tag="VFX38_03785"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314016.1"
                     /translation="MSRIGLVRLTLAVSLVAAASVALAAPSKTPDPFANLELRNLGPS
                     VAGGRVTTVNGLPGQPGIYYVGTAGGGLWKTSDDGMNWTELFQHSPTGSIGAVAIAPS
                     NPNDLWLGTGEANVRNDTLLGRGIFFSPDGGKTWQAKGLADAGQIAKIVVNPTNPDII
                     YVAVLGDPWKPTTTRGVYMTTDGGKSWNRMLFVDDTTGASAIVMDPANPNVMFAGMWT
                     EQRRPWTQINGSTKGGIWRSLDGGHTWSKLKGGLPTSDPTDRVALAVAPSDPNQVYAV
                     MATKSCILWGSSDMGDSWSCVSNNHALSVRPFYFSRIVVAPDNADTLYFASFQLMKST
                     DGGKTAKAIDDDVHVDHHAIWVDPKDPQRIAQGNDGGVYESLNGGGSWRYFDNLPIGQ
                     FYTVAISNTQPFGVCGGLQDNDGACGPSNSLDYAGIWGADWWDPIGGDGIYIVPAPSD
                     PSIVYADSEDGYAVRIDKRTMTSTSIRPTLASVSDTPTSKLQYRFNWGSPIAVSPTDP
                     NTVYMGSDVVFKSTDGGANWKPISKDLTRNDKSHQPVAGGPVMNDISGAETSDTILSL
                     AIAPTDPKVIWAGTDDGFVWVTKDDGQHWSNVTPGLPSSVKYGRTYQIGVSPFDAGTA
                     YITVDGHMLGDDHPYVFRTANYGAGWSGIAGGLPDNYSAMVVREDPNQKGLLALGTMR
                     GLYLSFDDGGHWTPMSANLPTMPVFDLKFSKSPHDLVLATHGRGLWILDNLQAIEQWS
                     PDVAKSDFHLFAASPGIEWVGYFGRHIGPAPTDFVADNPPNGPELAYYLQSALTKGAP
                     AKAAASGSSPVTIKVSDSAGNHVATLHGDGKAGINRIAWNMRYDGAKLPKFLQGQSFF
                     GGSGPSGPLALPGTYKISLSAGKQSATATVTVASDPRLHVSTQAQRSLLQTGLELRND
                     VNALVQVLDRIHTMVGILGDVSDATAKAKKGSAQAQLNAAAGKLKMQLGGFAMKLYNP
                     DVQFSVPEDTLHYVARFGMKVFGLYQNLSFVGPNQTLNAHQRREIAAAQAELNGYLAK
                     FNGPLHKAVVQFNGEAYKAGQQTLPVGKPIRIKPVKIPAS"
     gene            complement(6625..6966)
                     /locus_tag="VFX38_03790"
     CDS             complement(6625..6966)
                     /locus_tag="VFX38_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010219261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX5314017.1"
                     /translation="MKTVTLSVSSIEATKRQMAAAFLGERQGDHISFASMELLWKVLT
                     VKRWELLRAMTGQGAMTIRELARYVGRDVKAVHGEVHALLDAGVLDKTEDGRVVFPYD
                     AVHVEFTLKAA"
     gene            complement(6947..7249)
                     /locus_tag="VFX38_03795"
     CDS             complement(6947..7249)
                     /locus_tag="VFX38_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007294982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HEX5314018.1"
                     /translation="MKAEPLFRKRLALSETAFAEIVIHRLPKPLSGCRHDFKYRFALV
                     SEGVCVLRYDNEAGKGDHKHVAGRERAYRFVDLPGLQRDFWTDVEAWRNAHENRDA"
     gene            7455..7964
                     /locus_tag="VFX38_03800"
     CDS             7455..7964
                     /locus_tag="VFX38_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314019.1"
                     /translation="MSLVFSVLTAIVMPFVAYVLFGGWPAAIVVVGIYFLILLGAVIK
                     GLSGAVAIKQIPRDIGFGKISADHLYEYSRRRLETGVGVRCSSPSPLPSISMQATNVN
                     NSFDHPSDEDDEEILDVDDVAVPEIVRDHGRRFRKPVKYVVNLGNDEYLLFAEDGEWL
                     DGIHLTTHV"
     gene            8003..8599
                     /locus_tag="VFX38_03805"
     CDS             8003..8599
                     /locus_tag="VFX38_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314020.1"
                     /translation="MEGRIEFRTARVESGWSDFTVSLGESSWKCRASYISAHPLVDMI
                     HTAVDLYDHIFVAPMPEESSTWDITACDEPGGLLIRIVSIEGENRASVFRLDDQFKPQ
                     AVPVGEGTIDYWQFADAVFSDAARTVARYGFIGFRDRWFFPNWDRDYHYEALPIEHFL
                     YLAALIERRKPVNEITLADEIGLLRKIQKTSRDPRTVE"
     gene            complement(8985..9209)
                     /locus_tag="VFX38_03810"
     CDS             complement(8985..9209)
                     /locus_tag="VFX38_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEX5314021.1"
                     /translation="MTQHERIEINPEVMFGKPVIKGTRITVEQLLRKLAAGMTPEQII
                     EEHPQLLLEDIRAAEAFAADYMANEEVAFG"
     gene            complement(9356..>9669)
                     /locus_tag="VFX38_03815"
     CDS             complement(9356..>9669)
                     /locus_tag="VFX38_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314022.1"
                     /translation="LFRPSAPALDKVLDQFEPPKEGDLKEYSGRHYVCDYGTPAMHCD
                     DPWCWSQLCWIKNNLCLIYLDGGTYFRGVTLYPFHTHVDEEGMERVRAARIPRYLREP
                     G"
BASE COUNT         1813 a         3222 c         2894 g         1740 t
ORIGIN      
        1 ggcgctcgaa aaactcgccg acggctttcg ctggctcgaa gggccggtgt ggttcgccga
       61 cctgcaatgc ctgctgttca gcgacattcc gaataaccgc gtcatgcgct ggacggagtc
      121 gggcggcgtc tcggtctttc gcgaaccctc cgatttcgag aatggccaca cccgcgatcg
      181 cgaaggccgg ctcatttcct gttcgcatcg tgcgcgcgcg atccggcgca ccgaacctga
      241 cggcgcgacc acggtgctcg cggatcggca tcggggcaaa cgcctcaact cgccgaacga
      301 cgtggtcgtc aaatccgacg gctcgatctg gttttccgat ccgccctacg gcatccagac
      361 cgactacgaa ggcggcaaac aggaatccga acttccggcg aatctctatc gcctcgatcc
      421 gacgacgggc gaactcgcgg tcgtcgccga tgatttcgaa gggcccaacg ggctgtgctt
      481 ttcgcccgac gaaaaacgtc tctacgtcac ggaaaccgga ttgcagttcg cggaaaatcc
      541 gcagcgacat atccgcgtct tcgcggtcgc ggacgacgga cgacggctcg cgaaggggcg
      601 tgtgtttcac aaggtctcgc ccggcaacgc cgatggcatc cgctgcgacg aggacggcaa
      661 cgtctggtcg agcgccggcg acggcgtgca ctgcatagcg cccgacggca cgctgctcgg
      721 caagatcaag gtgccgagca tcgtgtccaa cctgtgcttc ggcgggcgcg agcgcagccg
      781 cctctttctc tgcgcctcgc aaaccctgta cgctgtgtat gtcaatcgcc gcggagcctg
      841 ccgaccatga atgacaatcg cggcccggtg cggcgtgtgc ttgcgctcag ccgcacggtt
      901 tccgatctcg atcgcgccgt cgccttttat cgcgaggcgc tcgacttttc gctgctcggc
      961 cgcgagcggc gcgagcagtc cgcctgggcg gaactgctgg ggcttccgcg cgttcgcggc
     1021 gaggtcgccc gcctcgggct cggcgaggag aaactcgaac tcgtcgcctg cgagccggcc
     1081 ggccgcgcct atccgccgga gagcggcgtc gccgaccact ggtttcagca tgtcgcgatc
     1141 gtggtctcgg acatggcggc cgcctgcgaa cggctcgatg cccgcccctt cgaggcgatc
     1201 agcgaaaacg ggccgcagca actccccgcg aacacgggct cggtcacggc ttacaagttt
     1261 cgcgatcccg acggccatcc gctcgaattg ctccacttcc cgacgggcag cggcgatccc
     1321 ggctggcagc gcaagcccgg gctctttctc ggcctcgatc actcggcgat cacggtcgcg
     1381 gatctgaacg aatcgctcga tttctacacc cgcctgctcg gcttcaccat cgccgggcgt
     1441 tcgctcaact ccgggccggc gcaggcgaac ctcgatcacg ccgccaacgt gcatgtgcgc
     1501 gtcgttgccc tgcaaccggc gaacgcggcc ggagcgccgc atctggaact gctctgctac
     1561 gagcgcccgg cgggtcaggc gatgctcccc gacaccaagc ccgacgatgt cttcgccgat
     1621 cgtctcttgc tcgaggtgag cgatcttccg gcgctcgtgc gcgtgctcga agcggaggcg
     1681 gtgtcctttc tgtcgcccgg catggtgcga ctcgccaacg gcgaacgtgc ggcgctcgtc
     1741 cgcgacccga ccggtcatct gctgctgctc agcgaatccg ccggggcggc gcaggcgcgc
     1801 tgaacggcgc agtctcccgc gacggatcgc gcgatgactc cggaaccgcg ggcgaattcc
     1861 gcaggacttg cgctttcccg catcgtcatc gtcggcgccg gcttcggcgg actcgcctgt
     1921 gcgcaggcgc tgcggcgggc gcgggcgaag gttacggtcg tggatcagcg caactttcat
     1981 ctcttccagc cgctccttta tcaggtggcg acggccgcgc tgtcgccggc ggagatctcg
     2041 atgccgatcc gccgcatcct gcgtcgacag gccaacagcc gcgtcgaact cgggcgcgtc
     2101 gccgcggtcg atcgaaatgc ccgttgcgtt cgtttcgaag cggacgatcg cgcgctcgcc
     2161 tacgattacc tcgtcgtcgc gactggttct actcatgcgt acttcggtca tgacgactgg
     2221 gcacggttcg cctcgcctct caagacgatc gaagacgcga cggcgatccg cggacacatt
     2281 cttcgcgcgc tcgaacgggc ggagctgagc gaagaggaaa gcgaacgcgc ccgcctcctg
     2341 agtttcgtcg tcgtcggcgg cggcacgacc ggcgccgaga tggcgggcgc gatcgccgaa
     2401 ctcgcgcgcg ccgaactcgc gatgccgcat cggcgcctgc acgggcgccg cgcccgcgtc
     2461 gttctgatcg aggccgggcc gcgtctcctt ccgtcctttc cgccggcgct cgcggccaaa
     2521 gcgcgccgct cgctcgaagg tctcggcgtc gaagtgctga cgcaacacgc cgtcgcgcac
     2581 tgtgacgatc gcggggtcat cgccgcgggg cggcgcatcg aggcggcgac cgtgatttgg
     2641 gcggccggag tcgccgcctc gcccgccgcc cgctggctcg acgccgacgc cgacaaggcc
     2701 gggcgcgtgc gggtcgcggc cgatctttcg ctccccggcg atccggccgt atttgtcatc
     2761 ggcgacagtg cggccgtttt caatcgcgac ggcagtcccg tgccgggcat ggcgccgccg
     2821 gcgaaacagg aaggcgccta cgtcgggcgc ttgctcgcgc gccggctcgc gggaaagccg
     2881 ccgcccgctc ccttccgtta tcgcaacctc ggcgatctcg cgaccatcgg gcgcaagtcc
     2941 gcgattctcg atctcggctg gctgcggctc agcggtttgc cgggctggct cgtctgggga
     3001 ggcgtgcaca tcttctttct catcggcttc cgcaatcgtt tcgtcgtcgc cttctcctgg
     3061 ctgtggtcgt acttcacccg cgactacggc gcgcgcctga tcaccggggg cgagcgcaaa
     3121 ccctgaagcg accggcatga cggccggcga cggcgtacaa tcggcccgag ttcgcggcgc
     3181 ggccgcggct tctctattct gtttcgtgaa cccgggagat caccatgtct cgcatcggcc
     3241 ttgttcgttt gacgcttgcg gtttctcttg ttgcggcggc aagcgtcgca ctggcggcgc
     3301 cctcgaagac gcccgatccc ttcgccaatc tcgaactgcg caacctcggc ccctcggtcg
     3361 cgggcgggcg cgtcaccacc gtgaacggcc tgcccggcca gcccggaatc tactacgtcg
     3421 gcaccgccgg cggcgggctg tggaagacca gcgacgacgg catgaactgg acggagctgt
     3481 tccagcacag ccccaccggt tcgatcggcg cggtcgcgat cgcgccctcg aatccgaacg
     3541 acctctggct cggcaccggc gaggcgaacg tgcgtaacga cacgctcctc ggccgcggca
     3601 tctttttctc gcccgacggc ggcaagacct ggcaggcgaa ggggctcgcc gatgcggggc
     3661 agatcgcgaa gatcgtcgtc aatccgacca atcccgacat catttatgtc gcggtactcg
     3721 gagatccctg gaagccgacg acgacccgcg gcgtctacat gaccaccgac ggcggcaaga
     3781 gctggaatcg catgctcttc gtcgacgaca ccaccggcgc gagcgccatc gtcatggacc
     3841 ccgcgaatcc gaacgtgatg ttcgcgggca tgtggaccga gcagcgccgc ccgtggacgc
     3901 aaatcaacgg cagcacgaag ggcggcatct ggcgctcgct cgacggcggt catacctgga
     3961 gcaaactcaa gggcgggctt cccacgagcg atccgacgga tcgcgtcgcg ctcgccgtcg
     4021 cgccttccga tccgaatcag gtgtacgcgg tcatggcgac caagagctgc atcctctggg
     4081 gcagcagcga catgggcgac agctggagct gcgtgagcaa caatcacgcg ctctcggtgc
     4141 gcccgttcta tttcagcagg atcgtggtcg cgcccgacaa cgcggacacg ctctacttcg
     4201 cctccttcca gctcatgaag tccaccgacg gcggcaagac cgcgaaggcg atcgacgacg
     4261 acgtgcatgt ggatcatcac gcgatctggg tcgatccgaa agacccgcag cgcatcgccc
     4321 agggcaacga cggcggcgtg tacgaaagcc tgaacggcgg cggaagctgg cgttacttcg
     4381 acaatctgcc gatcggccag ttttacacgg tcgcgatctc gaacacccag cccttcggcg
     4441 tctgcggcgg cctgcaggac aacgacggcg cctgcggacc ctcgaacagc ctcgactatg
     4501 ccggcatctg gggcgccgac tggtgggatc cgatcggcgg cgacggcatc tacatcgtgc
     4561 ccgcgccttc cgatccctcc atcgtctatg cggattccga ggacggctac gccgtgcgca
     4621 tcgacaagcg caccatgacc tcgacctcga tccgcccgac gctcgcgagt gtcagcgaca
     4681 ctccgacttc gaagctccag taccgcttca actggggctc gccgatcgcg gtctcgccga
     4741 ccgatccgaa caccgtgtac atgggctccg acgtggtgtt caagtccacc gacggcggcg
     4801 ccaactggaa gccgatctcg aaggacctca cgcgcaacga caagtcgcac cagccggtcg
     4861 ccggcggccc ggtgatgaac gacatctcgg gcgccgagac cagcgacacg attctttcgc
     4921 tcgcgatcgc gcccaccgat ccgaaggtca tctgggccgg aaccgacgac ggcttcgtat
     4981 gggtgacgaa ggacgacggc cagcactgga gcaacgtcac gccggggctc ccttcctccg
     5041 tgaaatatgg gcgcacctac cagatcggcg tctcgccctt cgacgccggc accgcctaca
     5101 tcaccgtcga cggacacatg ctcggcgacg accatcccta cgttttccga accgcaaact
     5161 acggcgcggg ctggagcggc atcgccggcg gcctgcccga caattattcg gcgatggtgg
     5221 tgcgcgagga cccgaaccag aagggactcc tcgccctcgg taccatgcgc ggactctatc
     5281 tttccttcga cgacggcggg cattggacgc cgatgagcgc aaatcttccg acgatgccgg
     5341 tcttcgatct gaagttcagc aagtccccgc atgatctggt gctcgcgacg cacggccgcg
     5401 ggctgtggat cctcgacaac ctgcaggcga tcgaacaatg gagcccggac gtcgcgaaga
     5461 gcgatttcca tctcttcgcc gcctcgccgg gcatcgaatg ggtcggctat ttcggccgcc
     5521 acatcggtcc cgcgccgacg gacttcgtcg ccgacaatcc gccgaacggt cccgaactcg
     5581 cctactatct ccaatccgcc ctcacgaagg gcgcgcccgc gaaggcggca gcgtcgggca
     5641 gctcgccggt gacgatcaag gtgagcgaca gcgccggcaa tcatgtcgcg acgcttcacg
     5701 gcgatggcaa ggcgggaatc aaccgcatcg cctggaacat gcgctacgac ggcgccaagc
     5761 tgccgaagtt tctgcagggc cagtcgttct tcggcggttc cggccccagc ggaccgctcg
     5821 ccctgcccgg cacctacaag atcagcctga gcgcgggcaa gcagagcgcg accgcgaccg
     5881 tgacggtcgc atccgatccg cgcctgcatg tctcgacgca ggcgcaacgc tcgctcctcc
     5941 agaccggact cgaactgcgc aacgacgtga acgcgctcgt gcaggtcctc gatcgcattc
     6001 acacgatggt cggaatcctc ggcgacgtga gcgacgcgac cgcaaaggcc aagaagggaa
     6061 gcgcgcaggc gcaactgaat gcggcggccg ggaagctcaa gatgcagctc ggcggcttcg
     6121 cgatgaaact ctacaacccc gacgtgcagt tcagcgttcc ggaagacacg ctgcactacg
     6181 tcgcgcgctt cggcatgaag gttttcggcc tgtaccagaa cctgagcttc gtcggtccga
     6241 accagacgct caacgcccat cagcgccggg agatcgccgc ggcgcaggcc gaactcaatg
     6301 gctatctcgc gaaattcaac ggcccgctgc acaaggccgt cgtccaattc aacggcgaag
     6361 cctacaaggc cggccagcaa acgcttcccg tcggcaagcc gatccgcatc aagcccgtga
     6421 aaatccccgc ctcctaggcc ggcacaaggc cgaagaccat tgccggcatc cggcgttccg
     6481 gatcgtcggc tctagcgatg attcccgaga acggcgattc gtcccgccgg cttcgccttt
     6541 ttgtaatagg cgaagtcggc gggaaaaggt tcccggaagc tcccgtcttc attcggcagt
     6601 tcatgcctgc cgaatttcat ttcttcatgc agccttgaga gtgaactcca catgcacggc
     6661 atcgtagggg aaaacgaccc gaccgtcttc ggtcttgtcc aatactccgg catccagcag
     6721 cgcgtgcacc tcgccgtgta ccgccttgac gtcgcggccc acataccgtg ccagctcgcg
     6781 gatggtcatg gccccttggc ccgtcatcgc ccgcagtaat tcccaacgct tgaccgtcaa
     6841 caccttccag agcaattcca tggaggcaaa ggaaatatga tcgccttgcc gttcgcccag
     6901 aaaagcagca gccatctgtc gtttggtcgc ttcgatcgaa gacacactaa gcgtcacggt
     6961 tttcatgcgc attcctccat gcttccacat ccgtccaaaa atctctttgc agcccgggca
     7021 aatcaacgaa acgataagca cgttcgcgac ctgcgacatg cttgtggtcg cccttcccgg
     7081 cctcgttgtc gtagcgcagc acgcacaccc cttccgacac cagggcgaac cggtacttga
     7141 agtcgtgtcg gcaacccgaa agcggcttcg gcagccggtg gataacgatt tcggcaaacg
     7201 ccgtttccga caaagccagc cgcttgcgaa acaacggttc cgccttcatg ttggagatca
     7261 taccaacacc caaaggccgt tgtcaattac tccaacacct gaggattttg gccgaaaaaa
     7321 cccgtccaat gcggagtttt ccacgaatac ggcgtgcctg cctgcagcgc ccttgcgtag
     7381 cacaggcatg cctacggtag ttgtcgcagg ctttgatcca tctcctggct aaaatcagag
     7441 ccggaggtga acccatgtcg ctcgtttttt cggtactcac cgcgatcgtg atgcccttcg
     7501 tggcctatgt gcttttcggc ggatggccgg ccgcaattgt cgtggtcggc atctacttcc
     7561 tcatcctgct cggagccgtg atcaaaggcc tttcgggggc cgttgccatc aaacagatcc
     7621 ccagagatat cggcttcggc aagatttcgg cggaccatct gtacgagtac tcgcgacgac
     7681 gtcttgagac aggggtgggt gtccgatgct cgtccccaag cccgcttcca tcaatttcca
     7741 tgcaggccac aaacgtgaac aattctttcg atcatccgtc tgacgaagac gacgaagaaa
     7801 tcctggacgt ggacgacgtg gccgtccccg aaatcgtccg cgaccacgga cgccggttcc
     7861 gcaagccggt caaatatgtc gtgaatctgg gcaatgacga atacctgctc ttcgcggagg
     7921 atggcgaatg gctcgacggt atccacctga cgactcacgt ttgagcccag actcagacgc
     7981 aaacaacaga aggtttgaaa aaatggaagg ccggatagag tttcgaaccg cgcgcgtaga
     8041 gagcggttgg tccgatttca ctgtcagcct cggcgaatcg agctggaagt gccgcgcttc
     8101 ctatatttcc gcgcatccgc tggtggatat gatccacacg gccgtggacc tttacgacca
     8161 tatcttcgtt gctcctatgc cagaggagag ctcgacctgg gatataaccg catgcgatga
     8221 accgggcggg ttgttgatac ggattgtctc gatcgaaggg gaaaaccgag cttccgtgtt
     8281 tcgactggat gaccaattca agcctcaagc cgtgcccgtc ggcgaaggca cgattgatta
     8341 ttggcaattc gccgatgccg ttttttcgga tgccgcccgc acagtggctc gctacggctt
     8401 tatcggattc cgcgatcgtt ggtttttccc caattgggat agagattacc actatgaagc
     8461 cctgcccatc gaacattttc tctaccttgc cgcattgatc gagaggcgga aaccggtaaa
     8521 tgaaatcacg cttgcagacg aaatcggcct gcttcgaaaa atccagaaaa cgtctcgaga
     8581 cccgcgcacg gttgaatagc gactaacgat caattcttgc gtttgccgca ggcggcgtcc
     8641 acggcgcgct cgactttggc gtggacaccg cacaaggctg gtatcgtgac tgaatcgcaa
     8701 agatcagcga ggacgcgcca gagaatttat cgacgatcgc ggggcgtgag aatcgagcca
     8761 ccgcacgaat cccaaacaac ggcgaagcac cacaacgcga agcaacccca tataatcgcc
     8821 gctgcaatcc agcccagaac gcctacgagg ggaaacatgg gcgaaataat gaagaggatc
     8881 agaaagcaga gcaacgcagt cctcccccgc taaaacccgc caaggcggaa aagcgactca
     8941 cacgggtcaa gtataagcca tctgtgatgg caggcggcgc ttgctcaccc gaacgcgact
     9001 tcctcgttcg ccatgtagtc ggccgcaaac gcttccgccg cccggatgtc ctcaagcagg
     9061 agttgaggat gttcctcgat gatctgctcc ggggtcatgc cggccgcgag cttgcgcagc
     9121 aactgttcca ccgtgatgcg cgtgcccttg attacgggct ttccgaacat gacttcggga
     9181 ttgatctcaa ttctttcgtg ttgagtcata gcggatcacc tctccggcga cgccgaacgt
     9241 tcgttcatca tacaacactc gatgcaaccg ctcgcaagtt agccttgcgg cgagacccct
     9301 tcacccgccg ttctcccgca agggtaaaag gagcaggaag ttgctgcaag tcgggttacc
     9361 cgggttcgcg caggtaacgc ggaatcctgg cagcgcgcac cctttccatg ccctcttcat
     9421 ccacgtgggt atgaaatggg tagagcgtca caccgcggaa gtatgtaccg ccatcgagat
     9481 aaataagaca caaattgttc ttgatccagc aaagctggga ccaacaccaa ggatcatcac
     9541 aatgcatcgc gggagtccca tagtcgcaga cgtagtggcg gccggagtat tccttcaggt
     9601 caccttcctt cggcggctcg aattgatcga gcaccttgtc caacgccgga gcggacgggc
     9661 gaaacaacg
//
LOCUS       DASUJG010000183         3165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_912121, whole genome shotgun sequence.
ACCESSION   DASUJG010000183 DASUJG010000000
VERSION     DASUJG010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912121"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1377)
                     /gene="rpsA"
                     /locus_tag="VFX38_03820"
     CDS             complement(<1..1377)
                     /gene="rpsA"
                     /locus_tag="VFX38_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018935518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX5314023.1"
                     /translation="MAESFAELLEESLGNVWVQPGSLVKARVVSIGPDYVLVDAGLKS
                     ESAIPTEQFKRENGELDVTAGDEVEVAVEAIEDGFGETRLSREKARRLRTWETLKSAF
                     ESGDNVTGYFSDKVKGGFSVDLEGIRAFLPGSLVDVRPVRDPSYLEGKPLEFKIIKLD
                     QRRSNVVVSRRAVVEKEYSAEREALMDRLNEGAVLKGVVKNMTDYGAFVDLGGIDGLL
                     HVTDIAWKRVRSPQEVIEVGQELEVKVLKFDRERNRVSLGLKQLTEDPWMDLPRRYPQ
                     GTRTFGHVTNITDYGCFVEIEDGVEGLVHSSEMDWTNRTVNPSRFVHIGQEVEVMVLA
                     IDPERRRVSLGMKQCTPNPWEDFAANHERGEHISGKIKSITDFGLFVGLDGDIDGLVH
                     LSDLSWNEPGEEVLRRYKKGEEVETVLIGVDTERERISLGIKQLGDDPFLNFLAAHPK
                     GGIVGAAVT"
     gene            complement(1594..2268)
                     /gene="cmk"
                     /locus_tag="VFX38_03825"
     CDS             complement(1594..2268)
                     /gene="cmk"
                     /locus_tag="VFX38_03825"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017916915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX5314024.1"
                     /translation="MKKPVAVLALDGPGGAGKGAVGRAVAARLGGWHYLDSGALYRVL
                     ALAASRRRLDAADTAAITAVAAALAVELDGGVVRLDGEDAGAAIRTEEVGRYASAIAG
                     AAPVRAALIARQRAMRRPPGLVADGRDMGTVVFPDAQTKVFLTASLEERARRRHKQLK
                     EQGIDANLSILTRELRERDGRDARRATAPLRPAADAVVVDTTAHPLAWVVARVLALVA
                     GGAPVR"
     gene            complement(2265..>3165)
                     /locus_tag="VFX38_03830"
     CDS             complement(2265..>3165)
                     /locus_tag="VFX38_03830"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="catalyzes the formation of
                     5-O-(1-carboxyvinyl)-3-phosphoshikimate from
                     phosphoenolpyruvate and 3-phosphoshikimate in tryptophan
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX5314025.1"
                     /translation="MGAAIHSDEGRAPLDIAARSGLHAIHYRLPVASAQVKSAILFAA
                     LAGDGESRIEDPFGTRDHAERMLPLFGARIEIAGATVALRPGPLAAAEVRVPGDLSAA
                     AFAIAAALLVPGSRLRIPNVGVNPGRDGCLRAFARMGARIARSKERSFGAEPVADLAV
                     ESQSLRGIRVPAEEVPRLIDEIPVLLVLAAVAAGETVIEGAGELRYKESDRIETMRAG
                     LEAMGAKIAVRGDDIMVRGGSLSRGGRVSSGGDHRVAMALALAGLVTPQPVRVTEAAW
                     IATSFPDFVATLRAAGIAVRAAA"
BASE COUNT          536 a         1103 c         1002 g          524 t
ORIGIN      
        1 cgtcacggcc gcgccgacga taccgccctt cgggtgcgcg gcaagaaagt tgaggaaggg
       61 atcgtcgccg agctgcttga ttccgagcga gatgcgttcg cgctcggtat cgacgccgat
      121 cagaaccgtc tcgacctcct cgcccttctt gtagcggcgc agcacttctt cgcccggctc
      181 gttccaggaa agatcggaga gatgcacgag gccgtcgatg tcgccatcga gtccgacgaa
      241 gagaccgaaa tcggtgatgg acttgatctt gccgctgatg tgttcgccgc gctcgtggtt
      301 ggccgcgaaa tcttcccagg ggttcggcgt gcactgcttc atgccgagcg acacgcgtcg
      361 gcgctccgga tcgatcgcga gcaccatcac ctcgacctcc tggccgatgt gcacgaagcg
      421 cgagggattg acggtgcgat tggtccagtc catttcggaa gaatgcacca gcccttcgac
      481 gccgtcttca atctcgacga aacagccgta gtcggtaatg ttggtcacgt gcccgaaggt
      541 gcgggtaccc tgcgggtagc gccgcggcag atccatccac gggtcttcgg tgagctgctt
      601 caggccgagt gagacgcggt tgcgctcgcg gtcgaacttg agcaccttga cttcgagttc
      661 ctgcccgacc tcgatcacct cctggggact gcgcacgcgc ttccaggcga tatcggtcac
      721 gtgcaggagc ccgtcgatgc cgccgagatc gacgaaggcg ccgtaatcgg tcatgttctt
      781 cacgacgccc ttgagcaccg cgccctcgtt caggcgatcc atgagcgcct cgcgctcggc
      841 gctgtattcc ttctcgacca ccgcgcggcg cgacaccacc acgttgctgc ggcgctgatc
      901 gagcttgatg atcttgaatt cgagcggctt gccttccaga taactcggat cgcgcaccgg
      961 gcgcacgtcc acgagcgatc ccggaagaaa cgcgcgaatc ccctcgaggt ccacgctgaa
     1021 gccgcccttg accttgtccg agaagtagcc ggtgacattg tcgccggatt cgaacgcgga
     1081 cttcagcgtc tcccaagtgc gcaggcggcg tgccttttcg cgcgagagac gggtttcacc
     1141 gaaaccgtcc tcgatcgcct cgaccgcaac ctcgacctcg tcgccggcgg tcacgtcgag
     1201 ttcgccgttt tcgcgtttga actgttcggt cgggatcgcg ctttcggatt tgaggcccgc
     1261 gtccacgagc acgtaatcgg gtccgatgga aacgactctg gcttttacga gcgagcccgg
     1321 ttgcacccag acgttgccca gactttcttc cagaagttcc gcaaaagatt cagccatcga
     1381 ttttctgtct ccggccggaa aaacgattcc ggctctgcca tgcttcaatc gctacggcgt
     1441 cgcctcctgc gacaccatcg caccggttgc gaacgacgtc cctgccgttc gacgaaaaag
     1501 cccggactgc ccccgcccgc aacggtcagg ggtccagaga aaaattccgg gcgaattttc
     1561 ctcggggaaa ctccgaaaca ttcagagctt ctttcaccgc acaggggccc cgcccgcgac
     1621 gagcgcgagc acgcgagcca ccacccaagc cagcggatgc gcagtggtat cgaccaccac
     1681 ggcatcggcc gcgggccgga gcggcgccgt cgcgcgtcgg gcatcacgcc cgtcgcgctc
     1741 gcgaagctcg cgggtaagga tggacagatt agcatcaatt ccttgttctt tcaactgttt
     1801 atggcggcgg cgggcgcgct cttccagcga cgccgtcaaa aacaccttgg tttgcgcatc
     1861 gggaaagacg accgttccca tgtcgcggcc gtcggcgacg aggccgggcg ggcggcgcat
     1921 cgcgcgctgg cgtgcgatca gcgcggcgcg cacgggcgcc gccccggcga tcgccgaggc
     1981 ataacgccca acttcttcgg tccggatggc cgcgcccgca tcctcgccgt cgaggcgcac
     2041 gacgccgccg tcgagttcga ccgcaagcgc cgccgccacg gccgtgatcg cggcggtatc
     2101 ggcggcatcg aggcgccggc gcgaagccgc gagtgcgaga acgcggtaca gcgctccgct
     2161 gtcgaggtaa tgccagccgc cgagacgagc ggcaacggcg cgcccgaccg cgcccttgcc
     2221 ggcgccgccc ggcccgtcca gcgcgagaac ggctaccggc ttcttcatgc cgcggcgcgc
     2281 acggcgatac cggccgcgcg aagcgtcgcg acgaaatcgg gaaacgaagt ggcaatccag
     2341 gcggcttcgg tgacgcgtac cggttgcggc gtgacgagac ccgcgagcgc gagcgccatc
     2401 gccacgcgat gatcgccgcc gctcgaaacg cgtccgccgc gggacaaact cccgccgcga
     2461 accatgatgt catcgccccg gaccgcgatt ttcgcgccca tcgcctcgag cccggcgcgc
     2521 atcgtctcga tccgatcgct ctccttgtag cgcaactccc ccgcgccttc gatcacggtt
     2581 tcgccggcgg cgacggcggc gagcaccaga agaaccggga tttcgtcgat gagccgggga
     2641 acctcctccg caggaacgcg gatgccccgc agggactgcg attccaccgc gagatcggcc
     2701 accggctccg cgccgaagct gcgctccttg ctgcgggcga tgcgggctcc catgcgggcg
     2761 aaggcccgca ggcaaccgtc gcggcccgga ttgaccccga cgttcgggat ccgcagccgc
     2821 gacccgggca cgagcagcgc cgccgcgatc gcgaacgccg cggcggaaag gtcgcccggc
     2881 acccgcactt cggccgccgc gagcggcccc ggtcggagcg cgacggtcgc acccgcgatt
     2941 tcgatccggg ctccgaacag cggaagcatc cgctccgcat gatcgcgcgt gccgaaggga
     3001 tcttcgatgc ggctttcccc atcgcccgcg agagccgcaa acaggatcgc cgatttcacc
     3061 tgtgcgcttg cgaccggcaa gcggtagtga atcgcatgca agccgctgcg ggctgcgatg
     3121 tcgagcggag cccggccctc gtcgctgtgg attgccgcac ccatc
//
LOCUS       DASUJG010000184         5704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1523871, whole genome shotgun sequence.
ACCESSION   DASUJG010000184 DASUJG010000000
VERSION     DASUJG010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5704
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523871"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VFX38_03835"
     CDS             complement(<1..632)
                     /locus_tag="VFX38_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008217146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase"
                     /protein_id="HEX5314026.1"
                     /translation="MLIRDRISRFWPAAATGALRVAFGVIWAVGAALTWSPEFAAHYV
                     GYLHNAAQGQPEWLAGWFAMWIGLVTPNAALFVWLTRIIESAIAFALLAGFARKTFYL
                     AGALFSLLVWSTAEGFGGPYTVGAANMGTAISYVLIFVALIGIDNREGRSPYSLDFFI
                     EKWWPRWRRIAEWGGPGALAREPQTLPWGLQIPALLGIAIIVVFLIGGLH"
     gene            complement(990..1076)
                     /locus_tag="VFX38_03840"
     tRNA            complement(990..1076)
                     /locus_tag="VFX38_03840"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1040..1042),aa:Leu,seq:cag)
     gene            1133..3433
                     /gene="rnr"
                     /locus_tag="VFX38_03845"
     CDS             1133..3433
                     /gene="rnr"
                     /locus_tag="VFX38_03845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEX5314027.1"
                     /translation="MSTKPRSRKSAAPRNRQAGTRGAPDWRRWREHDPARLDEARRYD
                     HPIPSRRLLRSYFEARAVPASLAAVAASFDLADAEREALAMRLGAMCRDGDLIENRRG
                     EFCLTRRLPVKTGTVLAHRDGFGFLAPDGGGDDVFLPPRTMRTLMHGDRAAVRVQRGR
                     DGREEGRLVDVLERGRSSLSGEYFQEHGVGFVRPDDRRLRDDVLIPGDARGAARPGEM
                     VTVELTDFPGAHGPAVGRVIEILGAREAAGMASELAIRNHQLPHEWPAAVAAEASALP
                     DALRAADLRERRDLRELPLVTIDGEDARDFDDALYCRRDGDGFRLHVAIADVSHYVRA
                     ASALDCEAEGRGNSVYFPDRVLPMLPEALSNGLCSLNPEVDRLCMVAELSVRRDGEVA
                     RSHFYPALMHSRARLTYTEVAAILVDGDKAARRKRKTILGELDNLYAVYRALAAARKR
                     RGAIDFDTVETRFVFDAAGAVTDLVPVVRNDAHRIVEECMIAANSAVARRLRRAKVPA
                     LYRVHEHPAADKLAELRDFLALHGLSLGGGDRPEPQHYAKLVKAIAERPDRLRIQTML
                     LRSLAQAIYAPEPGGHFGLALADYTHFTSPIRRYPDLVVHRALKRTISVSESGQSSGG
                     GDKLEALGRHCSMTERRADEATREAADMLKCQFMRERVGEVFAGVVSGVTGFGLFVEL
                     EGLYLEGLVHVTALPSDYWEFDPGSQRLRGSRSGASYAIGDHLRVKVARVSVEERKID
                     LVPAEATSPRRRTQAKAAGARARKRG"
     gene            3430..4170
                     /gene="rlmB"
                     /locus_tag="VFX38_03850"
     CDS             3430..4170
                     /gene="rlmB"
                     /locus_tag="VFX38_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX5314028.1"
                     /translation="MSDIACGVHAVRLALENGQVRRLWLDRRRRDRRIAELRELAQRR
                     GVAVGETESDELDRLAEGVRHQGVVAEIAGVAPRTENQFEAWLAAARDPLVLVLDGVE
                     DPRNLGACLRAADGAGVDAVVLPRRRAAGLTPAARKTAAGAAEALAIFTVTNLARTLD
                     TMGESGVHRVGAALASDAVAPWEIHLGGSLALVLGAEEAGLRRGTRERLDAVIALPMR
                     GVVESLNVAVACGALLYEALRQRDRSRR"
     gene            4277..>5704
                     /locus_tag="VFX38_03855"
     CDS             4277..>5704
                     /locus_tag="VFX38_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX5314029.1"
                     /translation="MSAESAHAGANARKIASLEGNERVNGEEGSREYDPASLPDYKPI
                     EAYGLIGDCRTTALVGEDGSIDWTCFPEFDSPAVFAAILDPTAGRFAIRPREPFSARQ
                     YYEYGTNVLVTRFATAKGVVRVRDFMPVIAERRMPASEIHRGVEGVSGDVPMEMIFEP
                     RFQYGGELPRFKSGSYGAIACHGKKNEQALALSCPVAPVVDQHGARAKFSVEGGDRLW
                     FVADWDSRMTHPVGAYRSEQRLELTRAFWHDWISKLAYHGNYRNQVERSLLAIKLLSA
                     GATGAVIAAPTTSLPEWIGGTRNWDYRYTWVRDSAFIMHAIFNAGYVSEGTQYFDWML
                     ERCVTDDSDLKVMYTVRGEPDIPERELKLRGYEDSQPVRVGNAAVEQFQLDIYGSLIE
                     AALHYQRVGGVLTMVEAERIASIVEFVRSRWREPDDGIWEARGHREHYTYSKIWAWVA
                     LDRGARLMRYLGLDMPWREWAAEAEV"
BASE COUNT          958 a         1892 c         1943 g          911 t
ORIGIN      
        1 ctgtgaagtc cgccgatgag aaacacgacg atgatggcga tgcccagcag cgccggaatc
       61 tgcaggcccc agggcagggt ctgcggttcg cgcgcgagtg cgcccgggcc gccccattcg
      121 gcgatgcgcc gccagcgcgg ccaccacttt tcgatgaaga agtcgaggct gtaggggctg
      181 cggccttcgc gattgtcgat gccgatgagc gcgacgaaga tcagcacgta gctgatcgcc
      241 gtgcccatgt tggccgcgcc caccgtgtac gggccgccga acccctcggc ggtgctccag
      301 accagcaggc tgaagagcgc gcccgcaagg tagaacgtct tgcgcgcgaa gcccgcgagg
      361 agcgcaaagg cgatcgcgct ttcgatgatg cgggtgagcc acacgaagag cgcggcgttc
      421 ggcgtgacga gcccgatcca catcgcgaac cagcccgcga gccactccgg ctgcccctgg
      481 gccgcattgt gcagatagcc gacgtagtgg gcggcgaatt ccggcgacca ggtgagcgcc
      541 gcgcccaccg cccagatgac gccgaaggcg acgcgcagcg cgccggtcgc cgccgccggc
      601 cagaatcggg aaatccgatc gcgaatgagc aggttcggca cgcgatcctc cgtcgggaca
      661 ccggcgcgta aagactcgcg cactatcgta acaatgttga gcccacgggc agcaacgtgc
      721 tttgatgtgg atcagaacca aacgagcggg gaaggagggc ggcgagcctc gcgaccgaac
      781 gcggcgggac gatcgcgcac ttcctgctct tttccggtat cggcgcgcgt gccggccgaa
      841 cctcgcgatc ggatccccgg ccgggcggcg ggcacgatcg cgctcttccg gccgccaacc
      901 agtcgctttc gcgtgaaaga cgtttcgcgc ggaaaccggc gacggccgcg aggctgttat
      961 gctgttcgcg cggcccgaaa gcccgcgatt ggtgccgagg agaggactcg aacctccacg
     1021 aggaaaccct cactggcacc tgaagccagc gcgtctacca attccgccac cccggcacgg
     1081 gcaggcggga cgattcgtcc agcgcgtata ttttcccgaa gcgagcgaat gtttgtcaac
     1141 gaaaccccga tccagaaaat ccgcagcccc gaggaaccgg caggccggca cccgcggcgc
     1201 ccctgactgg cggcgctggc gcgagcacga tcccgcccgg ctcgacgagg cgcgccgcta
     1261 cgaccatccg atcccgagcc ggcgccttct gcgcagctat ttcgaggccc gcgccgtgcc
     1321 cgcttcgctc gctgccgtgg cggcttcctt cgatctcgca gacgcggaac gcgaggcgct
     1381 cgcgatgcgc ctcggggcga tgtgtcgcga cggggatctc atcgaaaatc gtcgcggcga
     1441 gttctgtctt acgcgtcgtc ttccggtgaa gaccggcacg gtgcttgcgc accgcgacgg
     1501 cttcggtttt ctcgcgcccg acggcggcgg cgacgatgtg ttcttgccgc cgcgcaccat
     1561 gcgcaccctc atgcacggcg atcgcgccgc ggtacgcgtg cagcgggggc gcgacgggcg
     1621 cgaagaaggc cgtctggtgg acgtgctcga acgcgggcgg agcagtctca gcggcgagta
     1681 ttttcaggaa cacggcgtcg gattcgtgcg gcccgacgac cggcgcttgc gcgacgacgt
     1741 gctgattccc ggcgacgccc gcggcgctgc caggcccggc gagatggtca cggtggaact
     1801 cacggacttt cccggcgcgc acggtcccgc ggtcgggcgc gtcatcgaga ttctcggcgc
     1861 gcgcgaggcg gccggaatgg cgagcgaact cgcgatccgc aatcatcaac tgccgcacga
     1921 atggccggcg gccgtcgcgg ccgaggcgag cgccttgccg gatgcgctgc gcgccgcgga
     1981 tttgcgcgag cgccgggatc tgcgcgagct gccgctggtc acgatcgacg gcgaggacgc
     2041 gcgcgacttc gacgatgcgc tctactgccg ccgcgacggc gacggattcc ggctgcatgt
     2101 cgcgatcgcc gacgtgtcgc actacgtgcg tgcggcgagt gcgctcgact gcgaggcgga
     2161 gggccgcggg aattccgtgt atttccccga tcgcgtgctg ccgatgcttc ccgaggcgct
     2221 ctccaacgga ctctgttcgc tcaatcccga agtggatcgc ctgtgcatgg tcgccgagct
     2281 ttcggtgcgt cgcgacggcg aagtcgcgcg gagccacttt tatccggcgc tcatgcattc
     2341 gcgcgcacgt ctcacctaca ccgaggttgc ggcgattctc gtggacggcg acaaggcggc
     2401 gcgacgaaag cggaaaacga ttctgggaga actcgacaac ctttacgccg tctatcgcgc
     2461 actcgccgcg gcgcgaaaac ggcgcggcgc gatcgacttc gacaccgtcg agacgcgctt
     2521 cgtgttcgat gccgcgggcg cggtcacgga tctggtgccg gtcgtgcgca acgacgcgca
     2581 tcgcatcgtc gaggaatgca tgatcgccgc gaacagcgcg gtcgcgcgcc gcctgcgccg
     2641 tgcgaaggtg ccggcgctct atcgggtaca cgagcatccg gccgccgaca agcttgccga
     2701 gctgcgggat ttcctcgcgc tgcacgggct ttcgctcggc ggcggcgacc gccccgaacc
     2761 gcagcattac gcaaagctcg tgaaggcgat tgcggaacgt cccgatcgcc tgcgcatcca
     2821 gacgatgctg ctgcgctcgc tcgcgcaggc catctatgcg cccgagcccg gcggacactt
     2881 cgggctcgcg ctcgccgact atacgcattt cacctcgccg atccgccgtt accccgatct
     2941 cgtcgtgcat cgcgcgctga agcgcacgat ttcggtttcg gagtcgggac agtcgagcgg
     3001 cggcggagac aaactcgaag cgctcggccg tcactgttcg atgaccgaac gccgcgccga
     3061 cgaggcgacc cgcgaggcgg ccgacatgct caaatgccag ttcatgcgcg agcgcgtcgg
     3121 cgaagtgttc gcgggcgtcg tgagcggcgt caccggcttt ggcttgttcg tcgaactcga
     3181 aggtctgtat ctcgaagggc tcgtccacgt gaccgcgctt ccctccgatt actgggagtt
     3241 cgaccccggc agccagcggc tccgcggttc gcgctccggc gcgagctacg cgatcggcga
     3301 tcaccttcgc gtcaaggtcg cgcgcgtgag cgtcgaggag cgcaagatcg atctcgtccc
     3361 ggccgaggca acatcgccgc gtcgccggac gcaggcaaaa gcggccggag cgcgagcacg
     3421 caaacgcgga tgagcgacat cgcctgcggc gtacacgccg tgcgcctcgc gctcgaaaac
     3481 gggcaggtgc ggcggctgtg gctcgatcgg cgacgccggg atcggcgcat cgccgagttg
     3541 cgcgaattgg cgcaacggcg aggggttgcg gtcggcgaaa ccgaatcgga cgaactcgat
     3601 cgactcgccg aaggcgtgcg ccatcagggc gtggtcgccg aaattgcagg cgtcgcgccg
     3661 cgcacggaaa atcagttcga agcatggctg gccgcggccc gcgatccgct cgtgctggtg
     3721 ctcgacggcg tggaagatcc gcgcaatctc ggagcctgtc tgcgagccgc cgatggcgcc
     3781 ggcgtcgacg ccgtggtgct gccacgtcgc cgcgccgccg gactcacgcc cgccgcgcgc
     3841 aaaaccgccg ccggtgccgc ggaagcgctt gcgatcttca ccgtgaccaa cctcgcacgc
     3901 accctcgata cgatgggcga gtcgggcgtg catcgcgtcg gcgcagcgct tgcgtccgat
     3961 gccgtcgcgc cctgggaaat ccatctcggc ggatcgcttg cgctcgtgct cggcgcggaa
     4021 gaagcgggac tgcgccgcgg cacccgcgaa cgactcgacg ccgtgatcgc gctccccatg
     4081 cgcggcgtgg tcgaaagcct caacgtcgcc gtcgcctgcg gcgcgctcct gtacgaggct
     4141 ctgcgccagc gcgatcgttc tcgccgttga tgcgggacgg acgcgatgtg cttctgtgga
     4201 acactgcaaa ctgcgtagtt tcgcaggggc ggcgcggacc tttataatcg cgggagcagg
     4261 atgaatggag aaccgcatga gtgccgaatc cgcccacgcg ggcgcgaacg cgcgcaagat
     4321 tgcttcgctc gagggaaacg agcgggtgaa cggagaggag ggctcgcgtg aatacgatcc
     4381 cgccagcttg cccgattaca agcccatcga agcctacggc ctgatcggcg attgccgcac
     4441 cacggccctg gtcggcgaag acggctcgat agactggacc tgtttccccg aattcgacag
     4501 tccggccgta ttcgcggcca ttctcgatcc gaccgccggg cggtttgcga ttcggccccg
     4561 ggagccgttc agcgcccggc aatattatga atacggcacc aacgtgctgg tcacccgatt
     4621 cgccaccgcc aagggcgtcg tgcgcgtacg cgacttcatg ccggtgatcg cggaacggcg
     4681 catgccggcc tcggaaatcc atcgcggcgt ggaaggggtt tccggtgacg tgccgatgga
     4741 aatgattttc gagccgcgct ttcaatacgg gggagaactg ccgcgtttca aatcgggttc
     4801 ctacggcgcg attgcctgtc acggcaagaa gaacgagcag gcgctggcgc tttcctgccc
     4861 ggtcgcaccc gtggtggatc agcatggcgc gcgggcaaag ttcagcgtgg aaggcggcga
     4921 ccggctgtgg ttcgtcgccg actgggacag ccgcatgacg catccggttg gcgcctaccg
     4981 ctcggagcaa cgcctggagc tgacccgcgc cttctggcac gactggattt cgaaactcgc
     5041 ctaccacggc aattaccgca atcaggtgga gcgcagtctg ctcgcgatca aactgctgag
     5101 tgccggtgcg acgggggccg tcatcgcagc cccgaccacg agtctccccg agtggatcgg
     5161 cggcacgcgc aactgggatt atcgctacac ctgggtgcgc gattcggcgt tcatcatgca
     5221 cgcgattttc aatgcgggct acgtctccga gggtacgcag tatttcgact ggatgctcga
     5281 gcgttgcgtg accgacgatt ccgatctcaa ggtcatgtat accgtgcgcg gcgaaccgga
     5341 tattccggaa cgggaactga agctgcgggg ctacgaagat tcgcaaccgg tgcgcgtagg
     5401 caatgccgcg gtcgaacaat tccagctcga catctacggg agcctgatcg aggcggcgct
     5461 gcattatcag cgcgtcggcg gcgtgctcac gatggtcgag gccgaacgca tcgcaagcat
     5521 cgtcgaattc gtgcgcagcc gctggcgcga acccgatgac ggcatctggg aagcgcgcgg
     5581 ccatcgcgag cattacacct actccaaaat ctgggcctgg gtggcgctcg atcgcggcgc
     5641 gcgcctcatg cgctatctcg gactggacat gccctggcgt gagtgggcgg ccgaggccga
     5701 agtc
//
LOCUS       DASUJG010000185         2864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1278303, whole genome shotgun sequence.
ACCESSION   DASUJG010000185 DASUJG010000000
VERSION     DASUJG010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278303"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..1329)
                     /locus_tag="VFX38_03860"
     CDS             complement(130..1329)
                     /locus_tag="VFX38_03860"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HEX5314030.1"
                     /translation="MRAAGIMKSGLDPRAALVALFRDAIAQLRADRLVEAAMTGADFS
                     HVIALGKAGEALAAGAWRALGRRLASGFLAVPRGYESAELPPAAPFERHRGAHPIPDA
                     SSVAAGLALQCFVASLPASSRVAILLSGGASACIELPNKDVNLVFLRRVNEWLLASGL
                     PIMEVNRVRAGLSRLKGGGLARLLARVQAEAWVLCDIPGGDIGWVGGAPLTAPPPGML
                     AALPEWLAAQVRVTSAAPPSVRLHRLAGNEDAVARIEERGASAGPPLEGEAEELGAGI
                     GRALARAAPGLHVRGGEPVVALPAEPGRGGRCQQLALAAAAAFAGRKDCWLLAAGTDG
                     RDGFTEAAGACVDGGTIARGLALGLDAGSALRTADAGSFLAASGDLLFTGPTGTNIND
                     LVIALRA"
     gene            1489..>2864
                     /locus_tag="VFX38_03865"
     CDS             1489..>2864
                     /locus_tag="VFX38_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015231205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HEX5314031.1"
                     /translation="MSQLVGDFLLQRLGAWGVKRIFGFPGDGINGILGALNRAGDAFE
                     FVQVRHEEMAAFMACAHAKFTGEVGVCLATSGPGAIHLLNGLYDAKKDHQPVLAIVGQ
                     QAQAGLGGDYQQEVDLDSLFKDVAGEYVHLATSPAQVRHLVDRAMRIARAERRVTALI
                     IPNDLQSQKAVETPPRSHGTVHSGVGYSPPRIVPTEADLKRAAAVLNEGKKVAMLVGA
                     GALGATDEVIETAEKLGAGVAKALLGKAAVPDDLPWVTGSIGLLGTNPSWELMTGCDT
                     LLMVGSAFPYSEFLPEEGQARGVQIDISGRMVSIRYPMEVNLIGDSAETLRALLPHLE
                     RKEDRSWREEIEKNVAEWWKVLEARAMAEADPINPQRVFWELSKRLPDNCILACDSGS
                     AANWYARDLKMRRGMMASLSGGLATMGPGVPYAIAAKFAHPERSVIAMVGDGAMQMNG
                     INELITIS"
BASE COUNT          509 a          958 c          962 g          435 t
ORIGIN      
        1 ccgtcaggcg aatccgcacg gtctcgctcg tgcccgcggt ctggcccgtg ccgtcgttcg
       61 cgacgagcga gctgccgttg tggcaggccg ccagcgtccg gaggccgggt catacgcagc
      121 gacagacgct caggcccgga gtgcaatcac gaggtcgttg atgttggtgc ccgtcgggcc
      181 cgtaaagagc agatcgccgc tcgccgcgag aaagctgccg gcgtctgccg tccgcagcgc
      241 ggatccggca tcgaggccta gggccaaacc gcgggcgatc gtgccgccgt ccacgcaggc
      301 acccgccgct tcggtgaagc cgtcgcggcc gtcggttccg gcggcgagaa gccaacaatc
      361 cttgcggccg gcgaatgcgg cggcggcggc cagcgcgagc tgctggcaac gccctccgcg
      421 accaggttcc gcgggaagcg cgacgacggg ctcgcctccg cgcacatgca agcccggcgc
      481 ggcccgcgcc agtgcgcgac cgatgccggc ccccaattcc tcggcttcgc cctcgagcgg
      541 cggcccggca gaagctcccc gctcctcgat gcgtgcgacg gcgtcctcgt ttcccgcgag
      601 tcggtgcaaa cgaaccgacg ggggcgctgc gctcgtcact cgcacttgtg cggcgagcca
      661 ctccgggagc gccgccaaca tgccgggcgg cggcgccgtg agcggtgcgc cgccgaccca
      721 gccgatgtcg ccgccgggaa tatcgcagag cacccaggcc tccgcttgca ccctcgcgag
      781 cagacgcgcg agcccgccgc ccttgagacg cgacagcccg gcacgcacgc gattcacctc
      841 catgatcggc agaccgctcg cgagcaacca ttcgttgacg cggcggagaa aaacgagatt
      901 cacatctttg ttgggcaatt caatgcaagc ggaagcgccg ccggaaagaa gaatcgcaac
      961 ccgggagctc gcaggcaatg aggcgacgaa gcattgaagt gccaagccgg ccgcaacgct
     1021 cgaagcatcc ggaatggggt gcgcgccgcg gtggcgctcg aagggagcag ccggaggcag
     1081 ctcggcggat tcgtatccgc gcggcaccgc gagaaatccc gaagcgagcc gtcttcccag
     1141 ggctcgccac gcgcctgcgg cgagcgcctc gcccgctttg cccagggcaa tgacgtgcga
     1201 gaagtcggct cccgtcattg ccgcttcgac gaggcgatcg gcgcgaagct gcgcgatcgc
     1261 gtcgcgaaac agggcaacga gcgccgcgcg cgggtcgagc ccggatttca tgatcccagc
     1321 ggcccgcaca cgcgactcct cgattcgccg gtcgcggcca ctctagcagc ttgtcgaaaa
     1381 aggactgttc tgctgttttc cacgtcgcga attcggcaca tgtccgcgct cacggcgaag
     1441 gtgcgcgcgc gcacggatcg gcggggactg aaagcctaac attgacgcat gagtcagctc
     1501 gtcggggatt ttctgctgca gcggctgggc gcatggggcg tcaagcgcat cttcggtttt
     1561 ccgggcgacg gcatcaacgg aattctcggc gcgctcaacc gcgccgggga cgcgttcgaa
     1621 ttcgtgcagg tccgccacga ggaaatggcg gctttcatgg cctgcgcgca tgccaagttc
     1681 accggagaag tgggagtgtg tctcgccacg tcgggcccgg gcgcgattca tctgctgaat
     1741 ggcctctacg acgcgaagaa ggatcatcag ccggtgctcg cgatcgttgg gcagcaggcg
     1801 caggccggtc tcggtggcga ttatcaacag gaagtggatc tcgactcgct gttcaaggac
     1861 gtcgccggag agtacgtgca cttggcgacg agcccggcgc aggttcgtca tctggtggac
     1921 cgagcgatgc gcatcgcgcg cgccgagcgg cgcgtcaccg ccctgatcat tcccaacgat
     1981 ctgcaaagcc agaaggcggt cgagactccg ccgcgttcgc acggcacggt gcactcaggc
     2041 gtcggctatt cgccgccccg aatcgtgccg acggaagccg atctcaaacg cgccgccgcg
     2101 gtgctcaacg aaggcaaaaa ggtcgccatg ctggtcggtg cgggcgcgct cggcgccacc
     2161 gacgaggtca tcgaaacggc cgaaaagctc ggcgccggcg tcgccaaggc gctgctcggc
     2221 aaggccgccg tgcccgacga tctgccctgg gtcaccggct ccatcggcct gctcggtacc
     2281 aacccgagct gggagttgat gaccggctgc gacacgctgc tcatggtcgg ctccgcgttc
     2341 ccgtactcgg aatttctgcc cgaggaaggc caggctcggg gcgtgcagat cgatatttcc
     2401 ggacgcatgg tgagcatccg ctatccgatg gaagtgaatc tgatcggcga cagtgcggag
     2461 accctgcgag cactgctccc gcatctcgaa cgcaaggaag accgaagctg gcgcgaagaa
     2521 atcgaaaaga acgtcgccga atggtggaaa gtgctcgaag cgcgcgccat ggccgaagcc
     2581 gatcccatca atccgcagcg cgtattctgg gaactttcga aacgcctgcc ggataactgc
     2641 atactcgcct gtgattcggg ctcggccgcc aactggtacg cgcgcgatct caagatgcgc
     2701 cgcgggatga tggcttcgct ttccggcggc ctcgcgacca tgggcccggg cgtgccctac
     2761 gcgatcgcgg ccaagtttgc gcatccggag cgcagcgtca ttgcgatggt cggggacggc
     2821 gccatgcaga tgaacggcat caacgaactc atcacgatct cgaa
//
LOCUS       DASUJG010000186         4062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_667066, whole genome shotgun sequence.
ACCESSION   DASUJG010000186 DASUJG010000000
VERSION     DASUJG010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667066"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VFX38_03870"
     CDS             <1..211
                     /locus_tag="VFX38_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5314032.1"
                     /translation="VYTVRDGKISIRNVGVAREFGGLAVISKGLSKGDVVVVHVPRHL
                     AEGVAASARILPFAVSAAAAASAGD"
     gene            229..3312
                     /locus_tag="VFX38_03875"
     CDS             229..3312
                     /locus_tag="VFX38_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5314033.1"
                     /translation="MKRPVATTVLMAALVIFGFFAYRNLPVSELPNVDFPTIVVYANL
                     PGANPQTMASTVATPLERSFSSIAGLDSMSSVSGTGSTQITLQFDLERNIDAAAQDVQ
                     TAISESSRQLPRDMPSLPTLRKVNPGDYSILYIAFTAKHLALTKLDEYAETRVAQRLA
                     TLPGVADVNVWGSKKYAVRIFVNPDALNARGLSLSQVAAAVAAGNSNLPSGTLDSGNR
                     SYTVQTSGQLTDAAQYNDLVLAYNDGAPITLADIGHATNSIEENKRATSYNGEPAIVL
                     AVHRQPGANTVQVAGEARAILPELEREAPGGAQLNVIYDRSIFIRNSIHDVSYTLLIA
                     IAVVIAVIFLFLRNGSATLVSALAIPTSLVGTFALMYLFGFSLDNLSLMALVLAVGFV
                     VDDAIVVQENIVRHMDLGERPLAAAIAGSREISFTVLSMTLSLVAVFLPILFMGGVIG
                     RLFKEFAVTIAITILLSGLVSLTLTPMLSARLLRPARRHGRLYQWSERGFDRLRDGYL
                     RSLAWSVAHWRLTLAFATLILVLTGLLFVVVPKGFIPTEDTGAVFGNTEGPQGMSFAA
                     MNVQQNELVKRVRATPGVATVMSSAGQGRGGAAGENSGFLMIGLKPRGERSLSADDIV
                     SRLRREAAAVPNIEAFFQNPPAINTGTGGGQSTYNFVLQGSDLGELQRASESFQTQME
                     KIPGITDVDSDLELHNPQIDVRILRDKAAALGVTPAAIQTALASAYGGQQISTIYGAT
                     DEYQVILQLAPRFQQNINALDSLYVAGRDGTLVPLASIAEITPGVGPLSISHYQELPS
                     VTLSFDVRPGVSLGSVTKSIQSLAADMLPADVTGVFSGNAQIFQQSLTSLPILLLITI
                     LVIYMVLAILYEHFIHPITILTALPLAIFGALIALILFGQQLNIFSFVGLILLVGLVK
                     KNGIIMIDFALHARREQNLDAAAAILEACRIRFRPIMMTTVAAIFGTLPIAIGFGAGS
                     DARRPLGIAAVGGLVFSQFLTLYVTPAFYVAMEHLSERLRRRRAAATAADASHRA"
     gene            complement(3376..>4062)
                     /locus_tag="VFX38_03880"
     CDS             complement(3376..>4062)
                     /locus_tag="VFX38_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314034.1"
                     /translation="TKSPLRRPNCPRAGKHEVARGEEVAADFTVRQSSVVLYGLFPFN
                     RRNVVNLQTLKIARIAGLVLPAAFLICVTGSAFAADSSADSADLKAVANFRLDDAVIA
                     KFTQATKNIIALQKQHPELAKKMDAESTDADEQSIAAVVAFVKKYPPYSDAITRSGMS
                     IADYVTCSFALIQTGMYATMGGSDAGSVNTSGMSAVQAQNVKYFKANQAKFEALSKLM
                     DQLNDSDSDQ"
BASE COUNT          706 a         1398 c         1179 g          779 t
ORIGIN      
        1 cgtgtacacg gtacgcgacg gaaaaatctc catacgcaac gtcggcgtcg cgcgcgagtt
       61 cggcgggctc gcggtgattt cgaaggggct ttccaagggc gacgtcgtgg tcgtgcacgt
      121 tccgcggcac ctggccgagg gcgttgcggc gagcgcccgg attctcccct ttgcggtctc
      181 cgccgccgcg gccgcgagcg ccggcgactg aacctttccg ctcccttcat caagcggccg
      241 gtcgcgacca ccgtgttgat ggcggcgctg gtcatattcg gattcttcgc ctatcgcaat
      301 ctgccggtga gcgaacttcc gaacgtggac tttccgacga tcgtcgtcta tgccaatctt
      361 cccggcgcga acccgcagac gatggcgagc accgtggcca caccgctgga gagaagtttc
      421 agctccatcg ccggcctcga ttccatgagt tccgtaagcg gcacgggctc cacgcagatt
      481 accctccagt tcgatctcga acgcaacatc gacgccgccg cccaggacgt gcagacggcg
      541 atctcggaaa gttcgcggca actgccccgc gacatgccga gcctgccgac gctgcgcaaa
      601 gtgaacccgg gcgactattc gatcctctac atcgcgttca ccgcgaaaca tctggcgctc
      661 accaaactcg acgagtatgc cgagactcgc gtagcgcagc gcctcgcgac cctgcccggc
      721 gtcgcggacg tgaacgtatg gggttcgaag aaatacgccg ttcgcatctt cgtcaatccc
      781 gatgccctca acgcccgcgg gctttcgttg agccaggtcg cggcggcggt cgccgcggga
      841 aacagcaacc tgccctcggg aacgctcgac agcggcaacc gcagctacac ggtgcagacc
      901 tccggccagc tcaccgacgc cgcccaatac aacgatctcg tccttgccta caacgacggc
      961 gcgccgatca cgctcgccga catcggtcac gccaccaaca gcatcgagga gaacaagcgc
     1021 gcgaccagct acaacggcga gccggcgatc gtgctcgccg tgcatcgcca gccgggtgcg
     1081 aacaccgtgc aggtggccgg cgaggcgcgg gccatccttc cggaactgga gcgcgaagca
     1141 cccggcggcg cgcaactcaa cgtgatttac gaccgctcga tcttcatccg caactcgatt
     1201 cacgacgtga gctacacctt gctcatcgcg atcgccgtcg tgattgccgt catcttcctt
     1261 ttcctgcgca acggcagcgc cacgctggtg agtgcgctcg cgattccgac ctcgctcgtc
     1321 ggcacctttg ctctcatgta cctcttcggc ttcagcctcg acaacctttc gctgatggcg
     1381 ctcgtgctcg ccgtcggctt cgtcgtcgac gacgcgatcg tggtgcagga gaacatcgtg
     1441 cgccacatgg atctcggcga acggccgctc gcggcggcga tcgcgggcag ccgcgagatc
     1501 agcttcacgg tgctttccat gacgctgtcg ctggtcgcgg tcttccttcc gatccttttc
     1561 atgggcggcg tgatcggccg gctcttcaag gagttcgcgg tcaccatcgc gatcaccatc
     1621 ctgctttcgg ggctggtgtc gctcactctg acgccgatgc tctccgcacg cttgctccgt
     1681 cccgcgcgcc ggcacggccg actctatcaa tggagcgagc ggggattcga tcgcctgcgc
     1741 gacggatatc tgcgcagcct cgcctggtcg gtcgcgcact ggcgcctcac gctcgcgttc
     1801 gcaactttga tcctggtgtt gacagggctc cttttcgtcg tggttcccaa gggattcatc
     1861 ccgaccgaag acaccggcgc cgtcttcggc aataccgagg ggccacaggg catgtcgttc
     1921 gcggcaatga atgtgcagca gaacgaactc gtcaaacgcg tgcgcgcaac gcccggcgtt
     1981 gcaaccgtga tgtcgagcgc cggccagggc cggggcggtg cggccggcga gaattcgggc
     2041 tttctcatga tcggcctgaa gcctcgcggc gagcgaagtc tgagcgccga cgacatcgtc
     2101 tcgcgcctgc gccgcgaggc agccgcggtg ccgaacatcg aggccttttt ccagaatcct
     2161 ccagccatca acaccggcac gggcggcggg cagagtacct acaacttcgt gcttcaaggc
     2221 tcggatctcg gagagctgca acgcgcctcg gagtcgtttc agacgcagat ggagaaaatc
     2281 ccggggatca ccgacgtcga ctccgatctc gagctgcaca atccgcagat cgacgtgcgc
     2341 attctgcgcg acaaggccgc cgcgctcggc gtcacgccag ccgccatcca gaccgcgctc
     2401 gcgagcgcct acggcggcca gcagatcagc accatttacg gcgcgaccga cgagtatcag
     2461 gtgatcctgc aactcgcgcc gcgattccag cagaacatca acgcgctcga ttcactctac
     2521 gttgccggac gcgacggcac cctcgttccg ctcgcgagca tcgccgagat cacgccgggc
     2581 gtgggtccgc tcagcatcag ccactaccag gaactgccct ccgtcaccct gtccttcgac
     2641 gtgcgacccg gcgtctctct cggttccgtc acgaaaagca tccagtcgct tgccgccgac
     2701 atgctccccg ccgacgtgac cggagttttt tcgggcaacg cccagatttt ccagcagtcg
     2761 ctgaccagcc tgccgatcct gctgctgatc acgatcctcg tgatctacat ggtgctcgcc
     2821 attctctacg agcacttcat ccatccgata acgatcctca ccgcgctgcc gctcgcaatc
     2881 ttcggcgccc tgatcgcgct gattctattc ggccagcaac tcaacatctt cagcttcgtc
     2941 gggctgatcc tgctcgtcgg cctggtaaag aagaacggca tcatcatgat cgatttcgcg
     3001 ctgcacgccc gccgcgagca gaatctcgac gccgccgcgg ccattctcga ggcctgccgg
     3061 atccgctttc gcccgatcat gatgaccacc gtcgccgcga tcttcggcac gctcccgatc
     3121 gcgatcggct tcggcgccgg ctccgacgcc cgccgcccgc tcggcatcgc cgccgtcggc
     3181 ggtctcgtct tttcccagtt tctgacgctt tatgtcacgc cggcgttcta tgtcgcgatg
     3241 gagcacctga gcgaacggct gcgccgccgc cgcgccgccg cgaccgccgc ggacgcctcg
     3301 catcgtgcgt gacgcttggt gcgccattac ggcgcagtaa cagcgattcg cgccggagcc
     3361 agattcgtca accggtcatt gatcggaatc ggaatcattc aactgatcca tcaacttgct
     3421 cagcgcctcg aacttggcct ggttggcctt gaaatacttc acattctgcg cctgtaccgc
     3481 ggacatcccg gaggtattca ccgacccggc atcgctcccg cccatggtcg cgtacatgcc
     3541 agtctgaatg agcgcaaagc tgcacgtcac ataatcggcg atgctcatgc cggagcgggt
     3601 aatggcgtcg ctgtagggag gatacttctt cacgaacgca acgacggcgg cgatcgattg
     3661 ctcgtccgcg tccgtgctct cggcgtccat tttcttcgcc agttccgggt gctgcttctg
     3721 gagcgcgatg atattttttg tcgcctgcgt gaatttcgcg atgacggcat cgtcaaggcg
     3781 gaaattggcg accgccttca ggtcggcgga atcggccgaa ctatcggcag cgaaggcact
     3841 gcccgtgacg cagatcagga acgcggccgg cagaacgagg ccggcaattc tggcaatctt
     3901 gagcgtttgc aggttcacga catttctcct gttgaatgga aacaatccat aaagaacgac
     3961 actcgactga cgaaccgtga agtccgccgc aacttcctct ccgcgcgcga cctcgtgttt
     4021 accagccctt gggcagttcg gtcggcgcag cggtgatttc gt
//
LOCUS       DASUJG010000187         5921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1645921, whole genome shotgun sequence.
ACCESSION   DASUJG010000187 DASUJG010000000
VERSION     DASUJG010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5921
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1645921"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..753
                     /locus_tag="VFX38_03885"
     CDS             145..753
                     /locus_tag="VFX38_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314035.1"
                     /translation="MSKDYVERQFVRKQDMQEQDKIVELICESPESVSAALQTGLALQ
                     AAKNNLLRTLKGQLTHILSEKEWKLHIWEMEGAKRYAAFTIQYAESHRYEFCMEFEAG
                     GYSDLIYGICERGHHNPAQDLPNVKKALDRLPVVEGMSSEFWPWYTRIEEGEYKNWSD
                     HAFSGIVKNITANWIIEKANDVYRALDEGDCLDELKGPSPVA"
     gene            830..2986
                     /locus_tag="VFX38_03890"
     CDS             830..2986
                     /locus_tag="VFX38_03890"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5314036.1"
                     /translation="MGEKCVGALEVVARGNGRDAEPESDLPDEPRAVLYALRIVDRTD
                     CCDSLVERYRLSWVRELCAALEVVPPAEHSAAETAKFKEGVAFRLTQFEATAFHETVL
                     TRNLFVLSQQLGLSPLESDLLGLAALFKTQGWLYSLAELASHVSLASHVNSEQAVSLL
                     ATMVHASPAEVRRCLLARDAKLTRSGLLQVTSERADLPDKLGMISGLAGALLSPGESP
                     LEFLTSRCLERRAAELGAADFEHLDGEFELVERLVPTAVRRRTPGINLLIYGPPGTGK
                     TEFVRALAAKSGLTLWEVGHKDSEGNSASGGERLMQFCLAQALFAEDSRAAILFDEFE
                     DVLVPEESRNPFSPPRKISKAFLHDLLETNHVPTFWISNEPCWLDGAARRRFTHAIEM
                     QEPPRPVTERIFAAELSAFGLEAGWAARVSADERLVPAHAARVRRVLEHAEPPDPEAA
                     ARMAERVLGGCVALKGGGGPLRTELADAAGYRLGFLNADCDLRELARGIVRAGHGRLC
                     LEGPPGTGKTAFARHLARRLGRHLLIRRSSDLLGPFVGMTERLIAAAFVKAERDNAVL
                     LIDEIEGFLQTREGSRHSWEITQVNEMLTRLETYPGVFVATTNLARTLDVAARRRFDA
                     FVNFGYLRPAQTMMLLRATMKETSGEPPILNANQRRRLTAIPNLTPGDFAAIKRRAKL
                     FERSADIDWWIAALERDAAAKPDAPRSAPGFLAAVS"
     gene            3225..4004
                     /locus_tag="VFX38_03895"
     CDS             3225..4004
                     /locus_tag="VFX38_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX5314037.1"
                     /translation="MTVLAFDIETVPDVEGGKRLYGLDGLDDADVVRAMRTLRRQKTG
                     RDFIALHLQRVVAIACVLEDAKGLRVWSLGQADSPEEELLRRFYDGLREYQPTLVSWN
                     GGGFDLPVLAWRALIHGVNAAGYWEVGDRDREFRFNNYLSRYQWRHVDLMDVLSHYQL
                     RAAAPLDEIARLVGLPGKSGMHGSEVAEAWLAGRIESIREYCETDALNTYFIWLRFEL
                     LRGNLPADAYRERLEAARAMLAADGARPHLCEFARELGARQ"
     gene            4001..5299
                     /gene="rlmD"
                     /locus_tag="VFX38_03900"
     CDS             4001..5299
                     /gene="rlmD"
                     /locus_tag="VFX38_03900"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEX5314038.1"
                     /translation="MSAAAETVRIEGLSHEGRGVAHRDGKAVFVAGALPGETVRLGHE
                     RHHRRYDEADAAEVLAPAAERVAPCCPHFGVCGGCALQHLSPAAQIAAKQDSLLANLK
                     RLAGLEPRTLWPPLTGPAYTYRRRARLGLRYVQKKARVVVGFHEANGRYVTDSRVCPI
                     LVPPGDRLPEMLAQLVGGLDARERIPQVEFAAGDVAGALVFRLLGDASAADIARLIAF
                     GREQDLSIWLQTGGLETLRLLHGAPLLGYALPEFGLSLHFLPTDFVQVNAAVNRAMVA
                     AAIARLELGAKDRVLEFYCGIGNFSLALARSAGEVTSVEGDETLVRRARANAVANGIG
                     NVAFHVADLDAPMSDAPWLAEACDAVLLDPPRTGARALVPQLARLAPRRILYVSCHPA
                     TLARDGEALAAAGFGLAAAGVADMFPQTAHAEAMALFERE"
     gene            5306..5809
                     /locus_tag="VFX38_03905"
     CDS             5306..5809
                     /locus_tag="VFX38_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEX5314039.1"
                     /translation="MAIEIERKFLVASDAWRAQAAGEGERYLQGYLAIGERSVVRVRI
                     AGERRAWIGVKEHRIGRARGEYEYPIPVADARELLQLVIGRLVEKNRYRVPHAGQVWE
                     LDEFLGANAGLVVAEIELAAAEEEFERPSWLGAEVTAERAYYNAALALHPWTEWENAE
                     LRRNESE"
BASE COUNT         1060 a         1780 c         1969 g         1112 t
ORIGIN      
        1 gcatccgctc caaggccatg aaacgcatcg cctccagcgc ctcgtgcgag agcgtacgcc
       61 cgtccttttc atgcccatta ccctcattgc aagcctattc tatgacccac aatgactacc
      121 attagttccc taactttctc acccatgagt aaggattatg tggagcggca attcgtgagg
      181 aaacaggata tgcaggaaca agataagatt gttgagttga tatgcgaaag ccccgaatct
      241 gtgagtgccg cgctccagac gggtttggcg ctgcaagcag ccaagaacaa cctattgcga
      301 acactgaaag gacagctgac tcacattctg agtgagaagg agtggaaact ccacatttgg
      361 gagatggaag gcgcaaagcg gtacgcggca tttaccatcc agtatgctga aagccatcgc
      421 tatgaattct gcatggaatt tgaggccggg ggttacagcg atctcatcta tggcatttgt
      481 gaacgaggcc atcataaccc tgcacaggat cttccaaatg taaaaaaagc gttggatcgg
      541 ctcccggttg ttgagggcat gagtagcgag ttttggccgt ggtacacccg gatcgaagaa
      601 ggagaataca agaattggag cgaccacgct ttctcaggaa tcgtgaaaaa cataactgcc
      661 aattggataa tagaaaaagc taacgacgta tatcgggcgc tcgatgaagg cgattgtttg
      721 gacgaactca aagggccttc cccagtggcc tgaccggggt ctgagcaaga gcgacgcatt
      781 cggtcgccat cgaggcgcac aatgggcacg cttcataagg agccattgtg tgggcgagaa
      841 atgcgtcggg gcgctggagg tcgttgctcg gggtaacggg cgggacgctg agccagaatc
      901 cgatctgccg gatgagccgc gtgcggtcct ttacgcgttg cggattgttg atcggacgga
      961 ttgctgcgac tcgttggtcg aacgttatcg tttgagctgg gtgagggagc tttgcgcggc
     1021 cttggaagtg gttccgccgg ccgaacattc ggcggcggag actgcgaagt tcaaggaggg
     1081 ggtggctttt cgacttacac agtttgaagc gacggcgttt cacgagaccg ttctcacccg
     1141 caatctgttc gtgctgtcgc aacagctcgg attgtctccg ttggagtctg accttcttgg
     1201 tctggcagcg ctgttcaaga cacaaggctg gctttattcc cttgcggaac tggcttctca
     1261 cgtaagcttg gcttcccacg taaactcgga gcaagcggta tcgttattgg ccacgatggt
     1321 tcacgcctcg ccggcggagg tgcggcgctg tctgctggct cgggatgcga agttgacacg
     1381 ctcgggactg ttgcaagtca cgagtgaaag ggcggatctt cccgacaagc tggggatgat
     1441 ttcggggctg gccggggcgc tgttgtcgcc cggagaatcg ccactggaat tcctgacttc
     1501 acgatgcctc gaaaggagag cggcagagct gggcgccgcc gactttgaac atctggacgg
     1561 cgagttcgaa ctggtagagc gcctggtgcc gacggctgtt cgacgccgca ctccgggaat
     1621 caacctgctg atttatggtc cgcccggaac gggcaagacc gagtttgtgc gggcgctggc
     1681 tgcgaagtcc ggcctgaccc tgtgggaggt cggccacaag gattcggagg gcaacagtgc
     1741 ctcgggcggg gaacgcctca tgcagttttg cctggcgcag gctttgtttg cagaagattc
     1801 acgcgcggcg atcctgttcg acgagttcga ggacgtgctg gtgccggagg agtcgcgcaa
     1861 ccctttttct ccgccgcgca agatctccaa ggcatttctc catgatttgc tcgaaaccaa
     1921 ccacgtgccc acattctgga tcagcaacga gccgtgctgg ctcgacggtg ccgcgcggcg
     1981 ccgcttcacg cacgcaatcg agatgcagga accgccgcgt ccggtgaccg aaaggatatt
     2041 cgcggcggag ctttccgctt ttggcctgga ggccggctgg gcggcgcggg tcagcgcgga
     2101 tgaacgtctc gttccggccc atgcggcgcg ggtgcggcgg gtgctcgaac acgccgaacc
     2161 gcccgacccc gaggcggctg cccggatggc ggaacgagtt ctcggcggat gcgttgccct
     2221 gaagggcggc ggagggccac tgcggacgga actcgctgac gccgcgggtt atcgcctcgg
     2281 atttctcaat gccgattgcg atttgcggga attggcgcgc ggaatcgtcc gcgcgggaca
     2341 cggccggctg tgcctcgaag gaccgcccgg caccggcaag acggcctttg cacgccatct
     2401 tgcccgccgc ctgggacgcc atctgctgat tcgccgcagt tcggacctcc tcggcccctt
     2461 cgtgggcatg acggaacggt tgattgcggc ggcctttgtc aaggcggaac gcgataacgc
     2521 ggtgctgctg atagacgaaa tcgaaggctt tctccaaacc cgcgaaggat ctcggcacag
     2581 ttgggagatc actcaggtaa acgaaatgct cactcgtctg gaaacctatc cgggtgtgtt
     2641 cgtggcaacc accaacctcg cacgcacgct cgacgtggcg gcgcggcgac gcttcgacgc
     2701 cttcgtgaat ttcggctatt tgcgtccggc tcagacgatg atgctgttgc gcgcaacgat
     2761 gaaggagacg agcggtgagc ctccaatttt gaatgccaac cagcgccgtc gcttgacggc
     2821 gattcccaat ctcacgcctg gcgacttcgc tgcgataaaa cggcgggcca aactcttcga
     2881 acgatccgcc gacattgact ggtggatcgc cgccctggaa cgcgatgctg cggccaagcc
     2941 cgacgctccg cgctctgccc ccggcttcct ggccgccgtt tcctgaacgc cgcagcaaac
     3001 ggttcgggag gagagatata gacgcgtgaa tccgttgatt cgcgccttga gtaaagagcg
     3061 atgacgagcg gctccggatt gttgggtgac gcacagttcg aagcagaaga agacatggcc
     3121 gaaccacgcc ggcgagccgg gccgccgcca aggaacgagc ggacgatccg gggcgcttcc
     3181 ggcaccggtg atagactgca tccgacgttt ttcggacgtt tccgatgact gttctcgcgt
     3241 tcgacatcga gaccgttccc gacgtggagg gcggcaaacg gctctacggc ctcgacggcc
     3301 tcgatgacgc cgatgtcgtg cgcgcgatgc gcacgctccg ccggcagaaa accggccgcg
     3361 acttcatcgc gctccacctg caacgcgtcg tcgcgatcgc ctgcgtgctc gaagacgcga
     3421 agggcctgcg cgtctggtcg ctggggcagg cggattcgcc ggaagaggaa ctgctgcggc
     3481 gcttctacga cgggttgcgc gaataccagc cgacgctcgt gtcctggaac ggcggcggct
     3541 tcgatctgcc ggtgctcgcc tggcgcgcgc tcatccacgg cgtcaacgcc gccggctact
     3601 gggaggtcgg cgatcgcgat cgcgaattcc gtttcaacaa ttatctttcg cgctatcaat
     3661 ggcggcacgt ggatctcatg gacgtgctgt cgcactatca gttgcgcgcg gcggcgccgc
     3721 tcgacgagat cgcgcgcctg gtcgggttgc cgggaaagtc gggcatgcac ggttcggagg
     3781 tggcggaggc ctggctcgcg gggcgcatcg aatccatccg cgagtactgc gagaccgacg
     3841 cgctcaatac gtatttcatc tggctgcgct tcgaactcct gcgcgggaat ctcccggcgg
     3901 acgcataccg ggaacggctg gaggcggccc gcgccatgct cgcggcggac ggcgcacggc
     3961 cgcatctgtg cgaattcgcg cgggaactcg gcgcccggca gtgagcgcgg cggcggagac
     4021 ggtacgcatc gaagggctct cgcacgaagg ccgcggcgtc gcgcatcgcg acggcaaggc
     4081 cgtgttcgtc gcgggcgcgc ttcccggaga gacggtgcga ctcggccacg aacggcatca
     4141 tcgccgctac gacgaggcgg acgcggccga ggtgctcgcg cccgcggccg aacgggttgc
     4201 gccgtgctgt ccgcatttcg gtgtctgcgg cggctgcgcg ctccagcatt tgagcccggc
     4261 ggcgcagatc gcggcgaaac aggactcgct tcttgcgaac ctgaagcgcc ttgccggact
     4321 cgagccgcgg acgctctggc cgccgcttac cggaccggcc tacacctatc gtcgccgcgc
     4381 gcggctcggc ctgcgttacg tgcagaagaa ggcgcgggtc gtcgtcggtt ttcacgaggc
     4441 aaatgggcgc tatgtcaccg attcccgcgt ctgcccgatt ctcgttccgc ccggcgatcg
     4501 cctgccggag atgttggcgc agttggtcgg cgggctcgat gcgcgggagc ggattccgca
     4561 ggtcgaattc gcggccggcg acgtggcggg cgcgctggtg tttcgcctgc tcggcgatgc
     4621 cagtgccgcg gatatcgcgc gcctcatcgc cttcggtcgt gagcaggacc tttcgatctg
     4681 gttgcaaacc ggcggcctcg agaccttgcg ccttctccat ggcgcgccgc tgctcggtta
     4741 cgcgctgccc gagttcggat tgtcgctgca ttttcttccg accgatttcg tgcaggtgaa
     4801 cgctgcggtc aatcgcgcca tggtcgcggc ggcgatcgcg cgccttgaac tcggcgcgaa
     4861 ggatcgcgtg ctcgagttct attgcggcat cggcaacttc agtctcgcgc tcgcaaggtc
     4921 cgcaggagaa gtgacgagcg tcgaaggcga cgagacgctg gtgcgccggg cgcgcgccaa
     4981 cgccgtcgct aacggcatcg gcaacgtcgc ctttcacgtc gccgatctcg acgcaccaat
     5041 gtccgacgct ccctggctcg cggaagcctg cgatgcggtg ctcctcgatc cgccgcgcac
     5101 cggtgcccgc gcgctcgtcc cgcaactcgc gcggctcgcg ccgcggcgca ttctctacgt
     5161 gtcctgccat ccggcgacgc ttgcgcgtga cggcgaagcg ctcgccgccg ccggcttcgg
     5221 gcttgcggcg gccggagtgg cggacatgtt tccgcagacg gcgcacgcgg aagcgatggc
     5281 gctgttcgag agggaatgaa ccgcgatggc gatcgaaatc gagcgcaagt ttctggttgc
     5341 gagcgatgcc tggcgcgctc aggccgcggg cgaaggcgag cgttacctgc aaggctatct
     5401 tgcgatcggc gagcgctcgg tcgtgcgcgt gcggatcgct ggcgagcgcc gcgcctggat
     5461 cggggtgaag gaacatcgca tcggacgcgc gcgcggcgag tacgaatatc cgattcccgt
     5521 cgcagatgcg cgcgaactcc tgcaactggt gatcggtcga ctcgtcgaga agaatcgcta
     5581 tcgcgtcccg cacgccggtc aggtctggga actcgacgag tttctcggcg cgaacgcggg
     5641 actcgtggtt gcggaaatcg aactcgccgc ggccgaagaa gaattcgagc ggccctcctg
     5701 gctcggagcg gaggtgacgg ccgagcgcgc ctactacaac gcggcgctcg cgctccatcc
     5761 gtggacggaa tgggaaaacg cggaactgcg gcgcaacgaa agtgaatgaa atccgcatct
     5821 cgctcgccga gcaacgcctc gtgttcgaag ccgacggcgg aaggcgtgcc tatccggttt
     5881 cgacttcgca ttacggcgcg ggcgaggagc gcggaagttt c
//
LOCUS       DASUJG010000188         7854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1767461, whole genome shotgun sequence.
ACCESSION   DASUJG010000188 DASUJG010000000
VERSION     DASUJG010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7854
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1767461"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /gene="bfr"
                     /locus_tag="VFX38_03910"
     CDS             complement(<1..333)
                     /gene="bfr"
                     /locus_tag="VFX38_03910"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011063113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEX5314040.1"
                     /translation="MKGDKTVIQHLNAILKNELTAINQYFVHARMYKNWGLKELNEHE
                     YNESVDEMKHADRLIERVLFLEGLPNLQDLGKLHVGEDVKEILECDLKLEREAHPPLR
                     EAIAHCESV"
     gene            complement(432..638)
                     /locus_tag="VFX38_03915"
     CDS             complement(432..638)
                     /locus_tag="VFX38_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004657461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5314041.1"
                     /translation="MYVCICHGVTDRRIREAVDAGADSFETLQETLDVSTCCGCCAPE
                     VREILAEQLKGRRVPRLRIVAKSA"
     gene            complement(840..1430)
                     /locus_tag="VFX38_03920"
     CDS             complement(840..1430)
                     /locus_tag="VFX38_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314042.1"
                     /translation="MKYLSLPALLLAATTAGWASPPPQVVPAPSAPTAVWAIPAGLLQ
                     QAFPDIYGHLATANAPAMMKLDFNVQIPVLTGLQILKIALAAPSSGAATDASLIICRT
                     SPAAGVASAVLTCRTNAAALHRRRSVPVPDVSPECGIANLPVTAMRQTYREGIVPYAV
                     AAFFDFARVNPKALDRLVNGIPDPNAALCPTVRHLE"
     gene            complement(1531..2688)
                     /gene="dapE"
                     /locus_tag="VFX38_03925"
     CDS             complement(1531..2688)
                     /gene="dapE"
                     /locus_tag="VFX38_03925"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEX5314043.1"
                     /translation="MNGSDAVVELACELIRRRSLTPADGGCQELLGERLAPVGFALES
                     MPFGEVSNLWATQGNGAPLLVLAGHSDVVPPGPLENWTSDPFEPVLREGRLYGRGAAD
                     MKGGLAALVVAAINFVRVHPRHSGTLAFLITSDEEGPARDGTRRVAEALLARGLKIDH
                     CLIAEPSAVARAGDEIRIGRRGSLTAELMVSGVQGHVAYPDQADNAAHRLLGALGDLR
                     SQTWGKGDPAFPPLSFQIANLAAGTGADNVIPGEARARFNFRYPPPFTAAELKRCAQE
                     VVARQAPRHALHWQEGGHPFLSRDGALRAAVVAAIEAVTGTRPALGTGGGTSDGRFLA
                     PLGIEVVELGPVRESIHKADESVALSDLASLVAIYGSVLERVLAPSVRRSS"
     gene            complement(2685..3521)
                     /gene="dapD"
                     /locus_tag="VFX38_03930"
     CDS             complement(2685..3521)
                     /gene="dapD"
                     /locus_tag="VFX38_03930"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEX5314044.1"
                     /translation="MSPEEARGEIDSAFEVREHLGPDNATAAVREAVETAIALLDRGE
                     ARVAERGGDGWRINEWLKKAILLHFRLHRSETMDIGALRAWDKVPLKYGAYEAADFAR
                     DGVRVVPPATVRRGAFVARDCVLMPSYVNIGARIGAGSMVDTWATVGSCAQIGERVHI
                     SGGVGIGGLLEPLQAMPTIVEDDCFIGARSEIVEGVIVERGSVIGMGVYLGQSTRIYD
                     RRTRVVSFGRVPAGSVVVSGALPAKDGSHSLYAAVIVKHVDAKTRTKTALNELLRGSP
                     DE"
     gene            complement(3511..4716)
                     /gene="dapC"
                     /locus_tag="VFX38_03935"
     CDS             complement(3511..4716)
                     /gene="dapC"
                     /locus_tag="VFX38_03935"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011505868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEX5314045.1"
                     /translation="MNPDIALLKPYPFERLGLLLAGITAPADRAPISLSIGEPRHAPP
                     AFVVEALARHAGELGSYPTARGFPELRKAMGEALARRYRLGSAALDPDRQILPVSGTR
                     EGLFSFVQAVVSRRNDPLVVMPNPFYQIYEGAALLAGVRPWFLNTTAATGQKPDLAAV
                     PEKIWNHCALLFLCSPGNPGGAILDANEWKQAFQLADRYGFLIAADECYADLYTDDSR
                     PPPGALETALALGRDDFRRLVVFHSLSKRSSVPGLRSGLAAGDAAVIEPFARYRTYHG
                     CAMPIAAQRASIAAWNDDDYVAANRRRYQTKFAAAKRILAPVLPVEIPPGAFYLWLAV
                     PGGDDERFARELYRAEGLVTLPGRYLSRPTPEGDPGAGYLRVSLVPEEPICEEALHRL
                     ARFARSYES"
     gene            4955..5983
                     /locus_tag="VFX38_03940"
     CDS             4955..5983
                     /locus_tag="VFX38_03940"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX5314046.1"
                     /translation="MEPERPDAQFIFVVGASRSGTTMIARMLGRHSQICTFRELHFFD
                     QLWTRNEQSVDAARLAATLYSIQRKGYHEPRDLMAFQAEAAELVTGMTAEKRETSTAI
                     FREFLLQEAQHNGKRIPCEHTGHNVFYLDEIFALFPNSSVINVVRDPRAVLLSQKNWW
                     RRRMLGDSNLSKLHGLRQKVNYHPVTMGNLWNASVRAAGRYRDDKRVLQLNFEWCVED
                     GERLAQTLCQFLRLEFAPEMLKVPHVGSSLHRDRHDRTGVDASRASAWRDGGLTAAEI
                     RLCQRVVRRQLSPNGYEAVSVRGGLLGMLTLRLLWPLQALVAFVWNMRRVKHPLDAIR
                     RRLRRASA"
     gene            complement(6050..>7854)
                     /locus_tag="VFX38_03945"
     CDS             complement(6050..>7854)
                     /locus_tag="VFX38_03945"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX5314047.1"
                     /translation="GENRLLFDVQPRVAEMLGYRARSGALAVEALMQDYYRSAGAVAR
                     ALDLLAASLRENPGPTRPLETALVARGAFLDFDRRERPLEQPSLMLDVFTRWQREPGL
                     RALAPEARRAIAVALPRLDAQFRRNRENRERFLRLISAPQRVAAALAAMHEAGVLGRY
                     IPAFARISWRMQYDLFHIFTVDEHILRVVANVENLRGGRFEPAGGDLRHAAERIDCPE
                     VLYLAALFHDIAKGRDGDHSLLGAREARRFAADHGLAPSDAELVAWLVRNHLLLSLTA
                     QKSDLSDPAVIADFARKLGDQRRLDYLYVLTAADVRATNPALWNSWRQTLFAELYLAA
                     SRALWRGLDHPADSVSEIAARKRDARSALGGGNPAVSRLWRRFGENYFLQYTIEEIAW
                     HTRELLAGAGAPAVFLRPAPIGAGTSIAVYAARGIFAFARVTSVLGQLGLNVAAARCV
                     PIGNDETLDTYWVFENDGSTIEDPRRVERIEVLLAEELARESATRDRAIRPTPRQVRL
                     FDTPTRISFVESSAGRCGVFELHAGDHPGLLAAVARAFRRCDVYLLAARIMTVGERAE
                     DVFHVSDGDGRALDAGACTRLESALREEIASDHH"
BASE COUNT         1395 a         2403 c         2710 g         1346 t
ORIGIN      
        1 cacgctttcg cagtgcgcga tggcttcgcg cagcggcgga tgcgcctcgc gttcgagttt
       61 gagatcgcat tccaggattt ccttcacgtc ctcgccgacg tgaagcttgc cgaggtcttg
      121 caggttcggc agcccttcga gaaacaggac acgttcgatc agccggtcgg cgtgcttcat
      181 ctcgtccacg gattcgttgt actcgtgttc gttcagctcc ttgagccccc agttcttgta
      241 catgcgcgcg tgcacgaagt actgattgat ggcggtaagc tcgttcttga gaatcgcgtt
      301 cagatgctga atcacggttt tgtcgccctt catcgccgac tccctgcgcc tgttcgagtg
      361 gtagcaacag ggtagcgcag acgcgccttc ggagcaatga agcgcgggcg caggcccgtg
      421 cggcaatcgg gtcaggcgga tttggcgacg atccgcagac gcggcacgcg ccggcccttg
      481 agttgttcgg cgaggatctc ccgcacttcc ggtgcgcagc agccgcagca ggtggagaca
      541 tccagggttt cctgcagcgt ctcgaaactg tcggctccgg catccacggc ttcgcggatg
      601 cgccggtcgg taactccgtg gcagatgcag acgtacaaga ggcgaccgcg cgggaaactg
      661 ggatctattc tagacgcgaa tgcgaatcat tgtcaacaag cccggtgagg agaagctctc
      721 ggagggagag ggcggagcaa gtcaccggcc aagggctggc cgcggtcggc tgcgagccgc
      781 attgctgtcg gcgtcagtcc cgtgttgcgg gcaatcgtgc cgtcccggcc cgtggggcct
      841 tattcgagat gtcgaaccgt cgggcaaagc gctgcgttcg ggtccggaat tccattgaca
      901 agacgatcga gggccttggg gtttacgcgg gcgaaatcga aaaaggccgc gaccgcgtag
      961 ggaacgatgc cctcccgata cgtttgcctc atcgccgtca ccgggagatt cgcgatgccg
     1021 cattcgggcg acacgtccgg caccgggaca gatctccttc gatgcagggc cgccgcattg
     1081 gtgcggcagg taagcaccgc gctggcgaca cccgccgcgg gactcgtccg acagatgatc
     1141 agcgaagcgt cggtcgcggc gccggaggag ggagccgcga gcgcgatctt gagaatctgg
     1201 agtccggtca gcacgggaat ctgcacgttg aagtcgagct tcatcatcgc cggcgcattc
     1261 gcggtcgcga ggtggccgta gatatcgggg aaggcctgtt gcagcagacc ggccgggatg
     1321 gcccataccg ccgtgggcgc ggatggggcc ggtaccacct gcgggggcgg tgaagcccat
     1381 ccggccgtcg tcgcggccaa aagcaacgcg ggcagcgaaa ggtatttcat tgtcggctcc
     1441 tgttcgagca tgccatccca tgctacgccg gagccggcag cgattgcaga gcgcgatgaa
     1501 agcaacgact ccgcggccgc ggaagcgccg tcaagaagac cggcgaacgg agggcgccag
     1561 gactcgttcg aggacggaac cgtagatcgc gaccaggctg gccaaatcgg aaagcgccac
     1621 gctttcgtcg gccttgtgga tcgattcgcg caccggcccg agttcgacga cttcgatgcc
     1681 gagcggtgcg agaaaacgac cgtcggaggt tccgccgccg gttccgaggg cgggccgcgt
     1741 cccggtcacg gcttcgattg cggcaacgac agcggcgcgc aaggcgccgt cgcgcgagag
     1801 aaacggatgt ccgccttcct gccagtgaag cgcgtgccgc ggtgcctgac gggcaacgac
     1861 ttcctgcgcg catcgcttca gttcggccgc agtgaagggc ggcgggtagc ggaagttgaa
     1921 gcgcgcgcgc gcttcgcccg gaatcacgtt gtcggcgccg gttccggcgg caaggttcgc
     1981 gatctggaac gacagcggcg gaaacgcggg atcgcccttt ccccacgtct gcgaacgcag
     2041 gtcgccgagc gcgccgagca ggcggtgcgc ggcgttgtcg gcctgatcgg ggtaggcgac
     2101 gtgcccctga accccgctca ccatcaactc cgcggtgagc gacccgcgac gaccgatccg
     2161 gatttcgtcg ccggcgcggg cgacggcgga gggttcggcg atcaggcaat gatcgatttt
     2221 caggccgcgg gcgagcagcg cttcggcgac ccggcgggtg ccgtcgcggg cggggccttc
     2281 ttcgtccgag gtgatcagaa aggccagggt gccggaatgg cgcgggtgaa cgcgcacgaa
     2341 gttgatggcg gcaacgacga gcgctgcgag cccgcccttc atgtcggcgg cgccacggcc
     2401 gtagagccgg ccctcgcgca gaaccggctc gaatggatcg ctcgtccagt tttcgagcgg
     2461 ccccggcggg acgacgtcgc tgtgccccgc gagaacgagc aacggtgcgc cgttgccttg
     2521 cgtcgcccag agattcgaca cttcgccgaa gggcatcgac tcgagcgcaa aaccgacggg
     2581 cgccaaccgt tcgccgagga gttcctgaca gccgccgtcg gcgggcgtga gcgatcggcg
     2641 gcgaatcaac tcgcaggcga gctcgactac cgcgtcgctc ccgttcattc gtcgggcgag
     2701 ccgcgcagaa gttcgttcag cgcggtcttc gtgcgggtct tcgcatccac gtgcttcacg
     2761 attaccgcgg catagagact gtgcgatccg tccttcgccg gaagtgcacc ggaaaccacc
     2821 acgcttccgg ccggcacgcg tccgaagctg acgacgcgcg tgcgccgatc gtagatacgc
     2881 gtggattgcc cgagatacac gcccatgccg atcacgctgc cgcgttcgac gatcacgccc
     2941 tcgacgatct cggagcgcgc accgatgaag cagtcgtctt cgacgatcgt cggcattgcc
     3001 tgcagaggtt cgagtaagcc gccgattcct acgccgccgg agatgtgcac gcgctcgccg
     3061 atctgcgcgc aggagcctac ggtcgcccag gtatccacca tgctgcccgc gccaatgcgg
     3121 gcgccgatgt tgacgtagct cggcatgagc acgcagtcac gcgcaacgaa ggcaccgcgg
     3181 cgcaccgttg ccggcgggac gacgcgcacg ccgtcgcgtg cgaagtctgc cgcttcatac
     3241 gctccatatt tgagcggtac cttgtcccag gcgcgcaagg ctccgatgtc catcgtttcg
     3301 ctgcggtgca ggcggaaatg gagaaggatc gccttcttca gccattcgtt gatccgccag
     3361 ccatccccgc cgcgttctgc gacccgcgcc tcgccgcgat cgagcagcgc aatcgccgtt
     3421 tccaccgctt cgcgtacggc ggctgtggcg ttgtcgggcc cgaggtgctc gcgcacttcg
     3481 aacgcagaat cgatttcgcc gcgcgcttcc tcaggactca tacgatcgtg cgaagcgggc
     3541 gaggcgatgc agcgcttctt cgcagatcgg ctcttcgggc acgagcgaaa cgcgcagata
     3601 ccccgcgccc ggatcgccct cgggagtcgg gcgcgagaga tagcgtcccg gcagggtgac
     3661 gaggccctcg gcacgataca gttcgcgcgc gaagcgttcg tcgtctcctc ccggcaccgc
     3721 gagccagaga taaaaggcac cgggcggaat ttcgaccgga agaaccggcg cgagaatccg
     3781 ctttgcggcc gcgaacttcg tctgataacg ccggcgattt gcggcgacat agtcatcgtc
     3841 gttccaggcc gcgatgcttg cgcgctgcgc cgcgatcggc atcgcgcagc cgtgataggt
     3901 gcgatagcgc gcgaacggtt cgatgacggc ggcgtcgccc gcggcgaggc ccgagcgcag
     3961 ccccggaacg ctcgaacgct tggagaggct gtgaaagacg acgagacggc gaaaatcgtc
     4021 gcggccgagc gcgagggcgg tttcgagcgc acccggcggc gggcgcgaat cgtcggtgta
     4081 aaggtcggca tagcattcgt cggcggcgat gaggaagccg tagcgatccg cgagttggaa
     4141 ggcttgtttc cattcgttgg cgtcgaggat ggcgccaccc ggatttccgg gcgagcagag
     4201 aaacaaaagc gcgcagtggt tccagatttt ttccggcacg gcggcgagat cgggtttctg
     4261 tcccgtcgcg gccgtggtgt tgaggaacca gggccgcacg ccggcgagca gcgctgcgcc
     4321 ttcatagatc tggtagaagg gattgggcat cacgacgagc ggatcgttgc ggcgcgagac
     4381 gaccgcctgc acgaaggaaa acaatccttc gcgggtaccg ctgaccggca aaatctgccg
     4441 atccggatcg agcgcggcgg agccgagccg ataccgtcgg gcaagcgcct cgcccattgc
     4501 cttgcgcagc tccgggaagc cgcgggcagt gggatagctc ccgagttcgc cggcgtggcg
     4561 cgcgagcgct tccacgacaa aggcgggcgg cgcatgcctc ggttcgccga tcgacaggga
     4621 aataggggcg cgatccgcag gcgccgtgat cccggcaagg agcaggccaa gccgctcgaa
     4681 agggtagggt ttcaagagag cgatatccgg attcatcttg ctcgcgcgac tccggcacgg
     4741 ttacacggcg cgggcaattt tctcacaaat gcccgaacgt ctcagccgcg gacggtggca
     4801 ggcctgtcat ggcgacaaga gccgcggtga cctttcttca cccgggttgg cccggtcgcc
     4861 ccgggccttg ttcctctgcg ctgggaaatg caatggcagc acggccttct gtatcatagc
     4921 ggccttcgca cgatctggaa tcgggagcgg caccatggag ccggaaaggc cggacgccca
     4981 gtttattttc gtggtgggcg cgagtcgcag cgggaccacg atgatcgcgc ggatgctcgg
     5041 acggcattcg caaatctgca cgtttcgaga gttgcatttt tttgaccaat tgtggacccg
     5101 aaacgagcag tcggtggatg cggctcgttt ggcggccacg ctgtacagca ttcagcggaa
     5161 aggctaccac gagccgcggg atctgatggc ttttcaggcc gaggctgccg aactcgtaac
     5221 aggaatgacg gcggaaaaac gggaaacttc gaccgccata ttccgggaat ttcttttgca
     5281 ggaggcgcag cacaacggca agcgcattcc ctgcgagcat accgggcaca acgtttttta
     5341 tctggacgag atattcgctc ttttcccgaa ttcttccgtc atcaacgtgg tgcgggatcc
     5401 gagagcggtg ctgttgtccc agaagaattg gtggcggcgg cggatgttgg gcgattccaa
     5461 tctttcgaaa ctgcatggcc tgcggcaaaa ggtcaactat catcccgtga ccatgggcaa
     5521 tctgtggaat gcttcggtcc gagcggcagg acgttaccgg gacgacaaac gggtcctcca
     5581 gttgaatttc gaatggtgcg tcgaagacgg cgagcggctg gcacaaacgc tgtgccaatt
     5641 cctgcgcttg gagttcgcgc cggaaatgtt gaaagttccc catgtgggat cttcgcttca
     5701 ccgcgaccgc catgaccgca cgggtgtgga cgcgagccgt gcttctgcgt ggcgcgacgg
     5761 cggactgacg gctgcggaaa ttcgattgtg ccagcgggtc gtgcgtcgcc agttatcgcc
     5821 caatggctat gaagctgttt ccgtgcgagg cgggttgctg gggatgctga cgttgcgact
     5881 cctgtggccg ctgcaggcat tggtggcttt cgtctggaat atgcggcgtg tcaaacatcc
     5941 cctcgatgcg atcagaaggc gattgcgtag agcgtcggcc tgagcggttt ttatcatgcc
     6001 gatcactgtt cgtcgcttcc tgagatggtc ggcgaagcgc tgagcgactt cagtggtggt
     6061 cgctcgcgat ttcttcgcgg agggcggatt cgagcctcgt gcaggcaccg gcatcgagcg
     6121 cccgaccgtc gccatcggag acgtgaaaga catcctcggc gcgctctccg acggtcatga
     6181 ttcgggccgc gaggagataa acgtcgcagc ggcggaacgc gcgcgcgacg gcggcaagaa
     6241 ggccgggatg atcgccggcg tgcagttcga agacgccgca acgtcccgca gatgactcga
     6301 cgaaagagat gcgcgtcggt gtatcgaaga ggcgaacctg acgcggagtc gggcggatcg
     6361 cgcgatcgcg cgttgccgat tcacgtgcga gttcctccgc cagcaggact tcgatgcgct
     6421 cgacgcgacg cggatcctcg atcgtgctgc cgtcgttctc gaacacccag taggtgtcta
     6481 gggtctcgtc gttgccgatc gggacgcagc gcgccgccgc gacgttgagc cccagttgtc
     6541 cgagaacgga agtaacccgc gcaaaggcga aaattccgcg cgccgcgtag acggcgatgg
     6601 aggtgcccgc gccgatcggc gccgggcgca ggaagacggc gggagcgccg gcgccggcaa
     6661 gaagctcgcg cgtatgccag gcgatttcct cgatcgtgta ttgaagaaaa taattttcgc
     6721 cgaagcggcg ccacaagcgc gatacggcag gattgccgcc gccgagcgcc gaacgcgcat
     6781 cgcgtttgcg cgccgcgatt tcactgaccg aatccgccgg atggtcgagc ccgcgccaca
     6841 gcgcgcgact tgctgcgaga tagagttcgg cgaagagcgt ctggcgccag ctgttccaga
     6901 gcgccggatt ggtcgcccga acgtcggccg cggtgagcac gtagagataa tcgaggcgac
     6961 gctgatcgcc aagcttgcgc gcgaaatcgg cgatcacggc cgggtcggag agatccgact
     7021 tctgcgcggt gagcgagagc aggagatgat tgcgcacgag ccaggccacg agttccgcat
     7081 cgctcggcgc gaggccgtga tctgccgcga agcgtcgtgc ctcacgggcg ccgaggagcg
     7141 aatggtcgcc gtcccggcct ttggcgatgt cgtggaaaag agccgcgagg tagagaactt
     7201 ccgggcagtc gatgcgttcg gcggcgtggc gcaaatcgcc gccggccggt tcgaatcgcc
     7261 cgccgcgcag gttttcgacg ttcgcgacga cgcgcaaaat gtgttcgtcg acggtaaaga
     7321 tgtgaaagag gtcgtattgc atgcgccagc tgatgcgggc gaatgcaggg atatagcggc
     7381 cgagaacacc ggcttcgtgc atggcagcga gcgcggcggc gacgcgctgc ggcgccgaga
     7441 tcaggcgcag gaagcgctcg cggttctcgc ggttgcgccg gaattgggcg tcgagcctgg
     7501 gcagtgccac ggcgatggcg cgccgggctt cgggcgcaag cgcccgcagg ccgggttcgc
     7561 gctgccagcg cgtgaagacg tccagcatga ggctcggttg ttccaggggc cgttcgcggc
     7621 gatcgaaatc gaggaacgcc cctcgggcca cgagcgccgt ttccagtggc cgggtaggcc
     7681 cgggattttc ccgcaggctc gcggccagca gatccaaggc acgcgccacc gcgccggcgc
     7741 tgcggtagta gtcctgcatc agcgcctcga cggcgagcgc gcccgagcgt gcgcggtagc
     7801 cgagcatttc ggcgacacgc ggttggacgt cgaagaggag gcgattttcg ccgc
//
LOCUS       DASUJG010000189         5867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_788124, whole genome shotgun sequence.
ACCESSION   DASUJG010000189 DASUJG010000000
VERSION     DASUJG010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5867
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788124"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(475..551)
                     /locus_tag="VFX38_03950"
     tRNA            complement(475..551)
                     /locus_tag="VFX38_03950"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(515..517),aa:Pro,seq:ggg)
     gene            complement(579..935)
                     /locus_tag="VFX38_03955"
     CDS             complement(579..935)
                     /locus_tag="VFX38_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX5314048.1"
                     /translation="MLEAGSNGELPPIPAKRYFTIGEVSDLCDVKPHVLRYWEQEFPQ
                     LKPVKRRGNRRYYRHHEVLLIRQIRALLYEEGYTIGGARHKLAGDTARDDHNQSRQIV
                     RQLRSELEELLAVLAR"
     gene            complement(916..1233)
                     /locus_tag="VFX38_03960"
     CDS             complement(916..1233)
                     /locus_tag="VFX38_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEX5314049.1"
                     /translation="MSGADTQAVTKAELAEVLFDNLGLNKREAKELIEQFFEEISNAL
                     AAGDPVKLSGFGNFELRDKGRRPGRNPKTGEEIPVTARRVVTFRAGQKLKARVEGHAG
                     SGE"
     gene            complement(1220..3577)
                     /gene="pheT"
                     /locus_tag="VFX38_03965"
     CDS             complement(1220..3577)
                     /gene="pheT"
                     /locus_tag="VFX38_03965"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX5314050.1"
                     /translation="MRVPLSWLRSLCDAELGANALADRLTRQGLEVSAILPIAAPCER
                     VVAARFADVVAMPRSNFKRIETDAGGAGRFTVVSAAPNVRAGMLAALALPGAQLPDGR
                     LIGKREYAGQVSEAMICSAAELGLGESSDRLLELFADAPPGTPLAELYGLPDSALEIE
                     LTPNRADCFSILGIAREVHAFSGRPLVAPPIPGVTPAGSAAIAIDLETPSACPRYFGR
                     VINGLDTTAATPLWLAERLRRAGLRATHPVVDILNYVMLELGQPLHAFERESIRGSIR
                     VRRAHRGESLELLTGGCADLEEDMLVIADDKAPLALAGIMGGAASAVRPETRSVFLES
                     AWFAPAAIRGRARRLGLATEAAHRYERGVDPELPRRALERASELILAIAGGEPGPVVA
                     AESAAHLPLRAAIRFDPRAVERLAGFATTADEVRRTLERLGFAVELQDGLLAVTSPSF
                     RFDIECEADLVEEVARIEGFERIPAVAPLRRLPSPPTPPGDPDRVASLLVARGFSEAA
                     TMSFAARDVEELLAPDAGVPTVLANPLSEHESVLRRSLWTGLVQAAAYNLARQQERLR
                     LFEQGTVFTAGGERRQLALIACGSAAPEQWGVAARGLDFFDLKGLVESLLAAAGIVAA
                     SFVASDRRALAPGRRARAEAEGRSLAEFGVLAPALAARLKLPAATVLLELDLEALPLR
                     AVALAKPVPRFPSVRRDLALVLADTIPAAELLRIARRLGGDLLADVRIFDSYSGAAIG
                     EDRRSIGLALIFRDLSRTLTDAEVDGAVSAIMTGLEQELGAHVRS"
     gene            complement(3577..4602)
                     /gene="pheS"
                     /locus_tag="VFX38_03970"
     CDS             complement(3577..4602)
                     /gene="pheS"
                     /locus_tag="VFX38_03970"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX5314051.1"
                     /translation="MGNEEIERIREAALSAVAAVGDPAALDAVRVEYLGRKGRIKTLL
                     ASIGTLPAPERAMRGEALNALKGELAAALVARKEALVATEQTRAESEAAVDVTLPGRG
                     ETPGARHPITQSLARIEQWFVAAGFEIAAGPEIEDEHYNFEVLNIPADHPARALHDTF
                     YLADGRLLRTHTSPVQIRHLERAGSPPVWMIAPGRVYRRDSDLTHTPMFHQLEGLVID
                     DGIHMGHLKWLLGEFLRAFFGQELALRFRPSYFPFTEPSAELDVSCVRCGGAGCRVCG
                     GTGWLEILGCGMVHPRVLEGAGVDPARWSGFAFGLGVERLAMLRHGIADLRQLYENDL
                     RFLGQFA"
     gene            complement(4644..4994)
                     /gene="rplT"
                     /locus_tag="VFX38_03975"
     CDS             complement(4644..4994)
                     /gene="rplT"
                     /locus_tag="VFX38_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX5314052.1"
                     /translation="MARVKRGVSARRRHKKVLKQAKGYYGNRSKNWRTANQAVIKAGQ
                     YAYRDRRVRKRELRALWVVRINAAARAQGLSYSSFMHGLKRAGIELDRKILAHLALHD
                     AAAFAALVEKARGD"
     gene            complement(5007..5204)
                     /gene="rpmI"
                     /locus_tag="VFX38_03980"
     CDS             complement(5007..5204)
                     /gene="rpmI"
                     /locus_tag="VFX38_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX5314053.1"
                     /translation="MPKLKTHRGAAKRFRATAKGKFKRTQSHRRHILTKKSTKRKRQL
                     RHPALVADQDQHAVKRMLPYS"
     gene            complement(5387..>5867)
                     /gene="infC"
                     /locus_tag="VFX38_03985"
     CDS             complement(5387..>5867)
                     /gene="infC"
                     /locus_tag="VFX38_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012537293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX5314054.1"
                     /translation="TAPRVRVVGTDGQQIGVLTVEAAIQAAYNAELDLVEVAPTAEPP
                     VCRIMDYGRFVFEQNKKLQSNRRRQKQTQVKEIKFRPGTEEADYQIKLKKITEFLEEG
                     DKAKVSLRFRGREMLHQELGQELLARIRGDLEAAAVVEQMPKLEGRQMVMLLAPKKK"
BASE COUNT          946 a         1901 c         2005 g         1015 t
ORIGIN      
        1 gacatgtcaa tccccaacat cagcaccata tgccggtata gttcagagct tccatgagaa
       61 ggtgtgctga aatcggacta tacgccgcga aacgcgcctt gggaagaacc tgatccggcc
      121 cactttttac cgctcgcgcg caaggcggca caagagcagc gatcgttgcc ggcaaggggc
      181 accccttggt tccggtttgc acgcaatgcg gtgcttgtac gcccttgcct cccgccccgg
      241 agttcggccg cggttgcgcc tgatccatgg gccggcgagc acggggtcaa gcgaagcagg
      301 aatcggcggc gagccgaagc cgccactgcc accggccaca tcacagccct gtcggttttc
      361 ccggtcacgg taccgcgctc gtcgatcgcc gtgagcgtgc gattgacgct tgcgatggga
      421 tttacggtga agcgcgaatg cggagagcga ctgttcgcaa tcctttgatg gaattggtcg
      481 gggtgagagg atttgaacct ccgaccacct gcaccccatg caggtgcgct accaggctgc
      541 gctacacccc gaacgattta catgatagcg gaggcgcctc agcgcgcgag caccgcgaga
      601 agctcctcca gctccgaccg caactggcgc acgatctggc ggctctggtt gtggtcgtcc
      661 cgcgccgtgt ctccggcgag cttgtggcgg gcgccgccga tggtatagcc ctcttcgtaa
      721 agcagcgcgc ggatctggcg gatcagcagc acctcgtgat ggcggtaata gcggcggttt
      781 ccgcggcgct tgaccggctt gagctggggg aattcctgct cccagtagcg cagcacgtgc
      841 ggtttgacgt cgcagagatc gctcacttcg ccgatcgtga agtagcgctt cgccggaatc
      901 ggcggaagct ctccgttact ccccgcttcc agcatgtcct tccacccgcg ccttcagttt
      961 ctgccccgcc cggaaggtta ccacgcgccg cgcggtaacg ggaatttcct cgccggtctt
     1021 gggattgcga ccgggtcgcc ggcccttgtc gcgcaattcg aagttgccga atccggagag
     1081 cttcacggga tcgccggcgg cgagcgcatt cgagatttct tcgaagaact gctcgatcag
     1141 ttccttcgct tcgcgcttgt tcaggccgag attgtcgaag aggacctcgg cgagttccgc
     1201 cttggtcacc gcctgcgtgt cagctccgga catgtgctcc caactcctgt tcgaggcctg
     1261 tcattatcgc cgaaacggcg ccgtccactt cggcatcggt caaggtacgc gagaggtcgc
     1321 gaaagatcaa ggcgagcccg atgctgcgcc ggtcctcgcc gatcgccgcg ccgctgtagc
     1381 tgtcgaaaat gcgcacatcc gcgaggagat ccccgccgag cctgcgagcg atgcgcagga
     1441 gttccgcggc cgggatcgta tcggcgagga cgagggcgag gtcgcggcga accgagggaa
     1501 aacgcgggac cggtttcgcg agagcgaccg cgcgcaaggg caaggcttcg agatccagtt
     1561 cgagaagcac ggtggcggcc ggaagtttca ggcgcgcggc aagcgccggc gcgagaacgc
     1621 cgaactcggc gagcgatcgg ccttccgcct cggcgcgcgc gcgtctgccg ggagcgagcg
     1681 cgcgacgatc gctcgcgacg aagctggccg cgacgatgcc ggcggcggcg aggagcgatt
     1741 caacgagacc cttgaggtca aaaaagtcga gcccgcgcgc ggcaacgccc cattgttccg
     1801 gggcggcgct gccgcaggcg attagggcga gttggcgccg ctcgccgccg gccgtgaaca
     1861 ccgttccctg ttcgaagagt cgcaggcgct cctgttgacg ggcgaggttg taggcggcgg
     1921 cctgaacgag accggtccac agactgcgac gcagcaccga ttcgtgctcg gagaggggat
     1981 tggcgagaac cgtcgggacg cccgcatcgg gcgccagcag ttcctcgacg tcgcgggccg
     2041 cgaagctcat cgttgccgct tcggaaaatc cgcgtgcgac gagcagcgaa gccacccggt
     2101 ccggatcgcc gggtggcgtc ggcggggacg ggagcctgcg cagcggtgcg acggcgggaa
     2161 tccgctcgaa gccctcgatg cgtgcgactt cctcgacgag atccgcttcg cattcgatgt
     2221 cgaagcgaaa actcggcgac gtgaccgcca gcagtccgtc ttgcaattcg acggcgaagc
     2281 cgagccgttc gagggtccgc cggacctcgt ccgctgtggt ggcaaagccc gcgagacgtt
     2341 cgaccgcccg cgggtcgaag cgaatggctg cgcgcagagg caagtgtgcg gcggattcgg
     2401 cggcgacaac cggtcccggt tcgccgccgg caatggcgag gatgagttcg ctcgcgcgtt
     2461 cgagtgcgcg gcggggaagt tccggatcga cgccgcgctc gtagcgatgc gccgcctcgg
     2521 ttgcgagccc gagtcgccgc gcgcgtccgc gtatcgccgc gggcgcgaac caggcgcttt
     2581 cgagaaagac gcttcgggtc tcgggccgca cggcgctcgc cgcgccgccc atgattcccg
     2641 cgagtgcgag cggcgccttg tcgtcggcga tgacgagcat gtcctcttcg agatcggcgc
     2701 agccgccggt gaggagttcg agactttcgc cgcggtgcgc gcgccgcacg cggatcgagc
     2761 cgcgaatcga ttcgcgctcg aacgcatgca gcggttggcc gagttcgagc atcacgtagt
     2821 tgagaatgtc cacgaccgga tgcgtcgcgc ggagccccgc gcggcgcagc cgttccgcga
     2881 gccatagcgg ggtggcggcg gtcgtgtcga gcccgttgat gacgcgtccg aagtagcgcg
     2941 ggcatgcact cggtgtctcg aggtcgatcg cgatcgccgc ggagcctgcc ggagtgacgc
     3001 cgggaatggg cggtgcgacg agagggcggc cgctgaaggc atgcacctcg cgcgcgatcc
     3061 cgaggatgga aaagcaatcc gcgcgattgg gcgtgagttc gatttcgagc gcgctgtccg
     3121 ggagtccgta cagttcggcc agcggcgttc ccggcggcgc atcggcgaaa agttcgagca
     3181 agcggtcgga actttcgccg aggccgagtt ccgcggccga acagatcatc gcctccgaga
     3241 cctgtccggc gtattctcgc ttgccgatga gacgcccatc gggaagctgc gcgccgggga
     3301 gggcgagggc ggcgagcatc cccgctcgca cgttcggcgc ggcggacacg acggtgaagc
     3361 ggcccgcgcc gccggcgtcg gtctcgatgc gcttgaaatt gctccgcggc atggccacga
     3421 cgtcggcgaa gcgcgccgcg accacccgct cgcagggtgc ggcgatgggg agaatcgcgc
     3481 tcacctcgag gccctggcgg gtgagacgat cggcgagcgc attcgccccg agttccgcat
     3541 cgcagagcga gcgcagccag gaaagaggaa cacgcatcag gcgaactgcc cgagaaaacg
     3601 cagatcgttc tcgtagagct gacgcagatc cgcgatgccg tgacggagca tcgcgagccg
     3661 ctcgactccg aggccgaagg cgaaaccgct ccagcgcgcg ggatcgaccc cggcgccttc
     3721 gagcacccgc gggtgcacca tgccgcagcc gagaatttcc agccagccgg tgccgccgca
     3781 aacccggcag ccggcgccgc cgcaacgcac gcaggagaca tcgagttccg ccgagggctc
     3841 ggtgaagggg aaatacgagg gccggaaacg caaggcgagt tcctgtccga agaaggcgcg
     3901 cagaaattct ccgagcagcc atttgagatg gcccatgtgg atgccgtcgt cgatgaccag
     3961 cccttcgagc tgatgaaaca tgggcgtgtg ggtgagatcg gaatcacgtc gatatacgcg
     4021 ccctggcgcg atcatccaca ccggcggcga gccggcacgc tccagatggc ggatctgcac
     4081 cggcgaggta tgggttcgca ggaggcgtcc gtcggcgagg tagaaggtgt cgtgaagtgc
     4141 gcgcgccgga tgatcggccg gaatgttgag aacctcgaag ttgtagtgct cgtcctcgat
     4201 ttcgggcccg gccgcgattt cgaagccggc cgcgacgaac cactgctcga ttcgtgccag
     4261 actctgcgtg atcggatggc gggcgccggg cgtctctccg cggccgggaa gcgtgacgtc
     4321 gaccgccgcc tcggattccg ctcgcgtttg ctcggtcgcg accagcgcct ccttgcgcgc
     4381 gacgagcgct gccgcgagtt cgcccttcaa ggcattgagc gcttcccctc gcatcgcccg
     4441 ttcgggcgcc ggaagcgttc cgatgcttgc cagcaaggtc ttgatccggc ccttgcgtcc
     4501 gagatactcg acccgcaccg catcaagcgc agcgggatcg ccgacggctg cgaccgcgct
     4561 cagcgccgcc tcgcggatgc gctcgatttc ctcgttgccc atatttcgac tcgtgtaccg
     4621 cgcggttcgc gcgcgccggc ggcctagtcg ccccgagcct tctcgaccag cgccgcgaag
     4681 gcggcggcat catggagcgc gagatgggcg aggatcttgc gatcgagttc gatgccggcg
     4741 cgtttgagcc cgtgcatgaa gctgctgtag ctcagcccct gcgcgcgcgc tgccgcattg
     4801 atccgcacca cccacagcgc ccgcagttcg cgcttgcgca cgcgccggtc gcggtacgca
     4861 tactggccgg ccttgatgac ggcctgattc gccgtgcgcc agttcttgct gcgattgccg
     4921 taatagccct tcgcctgctt gagaaccttc ttgtggcgcc gcctcgcgct gacgccgcgc
     4981 tttactcttg ccatcgctct cgctcctcag ctgtagggaa gcatgcgttt gacggcatgc
     5041 tgatcctggt ctgcgaccag cgcgggatga cgcaactggc gtttgcgctt ggtgctcttc
     5101 ttggtgagga tgtggcgacg atgggattgg gtacgcttga acttgccctt ggccgtcgcg
     5161 cgaaagcgct tggccgcgcc ccgatgggtc ttcagtttcg gcatctgcgt gactcctctg
     5221 tctctccagg ccccggcagg tgccggcgtt tcagcccggg caaacgacgc gggccgcacg
     5281 gcggtgcggc ccgcaggatg gacggtctcg gccgtctttc aggggaaacg tcggcgctgc
     5341 cggcgacttc cacggggcat attgtacttc agggcgcctg cggaggctat ttcttcttcg
     5401 gcgcgagaag catcaccatc tggcggccct cgagtttcgg catctgctcg accacggccg
     5461 cggcctcgag gtcgccgcgg atgcgggcga gaagttcctg accgagttcc tggtggagca
     5521 tctcgcggcc gcggaagcgc agcgacacct tcgccttgtc gccttcttcc agaaactccg
     5581 tgatcttctt cagcttgatc tggtagtccg cctcttcggt gccggggcgg aacttgattt
     5641 ccttgacctg ggtctgcttc tggcggcgac ggttcgactg cagcttcttg ttctgctcga
     5701 acacgaaacg gccgtaatcc atgattcggc agaccggagg ttcggcggtc ggcgccactt
     5761 cgaccaggtc gagttcggcg ttgtaggcag cctgaatcgc ggcctcgacc gtgaggactc
     5821 cgatctgctg gccatccgtg ccgaccaccc ggactcgcgg cgcggtg
//
LOCUS       DASUJG010000190         2907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_545563, whole genome shotgun sequence.
ACCESSION   DASUJG010000190 DASUJG010000000
VERSION     DASUJG010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545563"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VFX38_03990"
     CDS             <1..519
                     /locus_tag="VFX38_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314055.1"
                     /translation="ALGYGWMMEADSYAPLANTWRLWDLAKPLVKGIILPNGEKQGAV
                     VYEVEHDPNWSVIAPDHVTWENAVPDSVTPVTRQYGCGGAANHTLGVRSHEVECFLFD
                     PSATPEDEQTLVVAPTMTDMDAPGGRDDYSKYPKGNLDPTGHYFFWLSNLGGDRLDAF
                     MVEIPYQKLTHR"
     gene            873..1508
                     /locus_tag="VFX38_03995"
     CDS             873..1508
                     /locus_tag="VFX38_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5314056.1"
                     /translation="MSMQRILLADDHLMFRQGIRALLEAQGWEIAGEASDGREAVRLA
                     RRTNPDIAVLDVGMPLLNGIDAAREMHRRTPATQTILLTMYEENSYVLESLEAGIRGY
                     VLKAQAAEDLVAAIRQVGQGGVYLSPGISETLIDAYRTKSSVSADPLTLRERQVLQLV
                     AEGKTTREIAEILTLSVKTADSHRTRMMKKLDIHDTAGLVRYAIRRGLIHA"
     gene            1543..2445
                     /locus_tag="VFX38_04000"
     CDS             1543..2445
                     /locus_tag="VFX38_04000"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5314057.1"
                     /translation="MTTDLARRETHFEFGENWLSFVNEISDARVLAATRDLERLIPAS
                     EIRGRRFLDIGSGSGLSMLAALRLGAREVVGIDIDENSVAASQRCLGRLAPGSRWQVF
                     RASVFDLDVAQTGSYDVVHSWGVLHHTGAMWAALDRAAALVSADGLLVFALYLKTPFC
                     GFWRAEKYFYSRVPGAVQAPIRWIYEGAGLLWDGASRTRRTIIAKDAPAGNDKTLPAR
                     GMSRSHDTHDWLGGYPYESATAAEVHDRLGALGFGIVRENLNEACLGNLFGSTNNEYV
                     ARRRTTARPQSAAAGGERTTFGAS"
     gene            2435..>2907
                     /locus_tag="VFX38_04005"
     CDS             2435..>2907
                     /locus_tag="VFX38_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314058.1"
                     /translation="MLPNKTAARFVLAAAALCPLAAQAAGGQTQTSGAEGFTVTPFVA
                     LATTYDDNLFGAAGAGEGDTILSVGPGLDLRYLSPRTEFRFSYSLAAAKYLHHSTLDS
                     WIDEQQGTLGIERKFTDRLSAGVDASYLETHDPERLTPTTGLVYGRTRATEETLRP"
BASE COUNT          538 a          948 c          932 g          489 t
ORIGIN      
        1 gcgctcggct acggctggat gatggaggcc gactcctacg ccccgctcgc caatacctgg
       61 cggctgtggg acctcgccaa gcccctggtg aaggggatta tcctgcccaa tggcgagaag
      121 cagggcgctg tggtctatga ggtcgaacac gatccgaact ggtccgtcat cgccccggat
      181 cacgtcacct gggagaatgc cgtacccgac tccgtcacgc ccgtcactcg gcaatacggc
      241 tgtggcgggg cggccaacca cacgctcggg gtgcgttccc acgaagtcga atgcttcctc
      301 ttcgatccct ccgcgacccc ggaagacgag caaactctgg tcgtcgctcc gacgatgacc
      361 gacatggatg cgccgggcgg ccgcgacgac tactcgaagt atccgaaggg caatctcgat
      421 ccgacgggcc attacttctt ctggctgagc aatctcggcg gcgaccgcct cgacgccttc
      481 atggtggaaa ttccctatca aaagctgacg catcgatagg aaagttctgt tttattccgt
      541 tgatctgaga taaggagcgc gacggaaatg ctttcccgtt ccggaagaag gcgatgaaag
      601 gaacaacttc ccccatccaa aggaaggggg atcgaggggg atgtcttctg cggctcgcgg
      661 cgcgcctgcc gcgcttgccc gcccccgact ttcctcccga gggcgggaag gagtacaaca
      721 agccggtcgt ctctcgtttt tccgaagttc cgggattcag gtgattgcct gattccttcg
      781 tcggtagaaa agccatggaa tagggaacag cggccgaggc aggcgcccca tcggggcgtc
      841 ggcgaaagcc tgcaaccctc gcggagccgg tcatgtccat gcagcgcatc ttgttggcgg
      901 acgatcatct gatgttccgc cagggaatcc gggccctgct ggaggcgcag ggctgggaga
      961 tcgcgggcga ggcgagcgac gggcgcgaag cggtgcgact cgcgcgccgc accaaccccg
     1021 acatcgccgt gcttgacgtc ggcatgccgc tcctgaacgg catcgatgcg gcgcgggaaa
     1081 tgcaccggcg cacgcccgcg acgcagacga ttctcctcac gatgtatgaa gaaaattctt
     1141 atgtgctcga atccctggag gccggcatcc gcggctatgt gctcaaggcg caggccgcgg
     1201 aagatctcgt cgcggccatc cgccaggtgg gacagggcgg cgtctatctc agccccggaa
     1261 tttccgaaac cctgatcgat gcctatcgca cgaagtcttc ggtgagcgcc gatccgctga
     1321 cgctgcgcga gcgccaggtg ctgcagctcg tggcagaagg gaaaacgacc agggaaatcg
     1381 cggaaatcct caccctgagc gtgaagacgg ccgattcgca ccgaacgcgg atgatgaaga
     1441 aactcgacat tcacgacacc gccgggctgg tccgttacgc gatccgccgc ggcctgattc
     1501 acgcgtgaac gcggttcgtc gcgaacctcg gcggagagcg tcatgacgac cgatctcgcg
     1561 cgacgcgaaa cgcacttcga attcggcgaa aactggctgt ccttcgtgaa cgagatatcg
     1621 gacgcccgcg ttctcgcggc gacccgcgac ctcgagcgcc tgattcccgc gagcgaaatc
     1681 cggggcaggc gctttctcga tatcggaagc ggctcgggac tcagcatgct tgcggccttg
     1741 cgcctcggcg cacgcgaagt ggtcgggatc gacatcgacg aaaattccgt ggcggcgtcg
     1801 caacgctgtc tcgggcgcct tgcccccggg tcgcggtggc aggtgttccg cgcgagcgtg
     1861 ttcgatctcg atgtcgcgca aacgggatcc tacgacgtcg tgcacagttg gggagtgctg
     1921 catcacaccg gcgccatgtg ggcggcgctc gaccgggccg ccgctctcgt cagcgccgac
     1981 ggtctgctgg ttttcgcgct gtatctgaag acgccgttct gcggattctg gcgcgccgag
     2041 aaatatttct actcgcgcgt tccgggggcc gtacaggcgc cgatccgctg gatctacgaa
     2101 ggagcggggc ttctctggga cggcgcctcc cgaaccagaa gaacgatcat cgcgaaggac
     2161 gcgccggcag ggaacgacaa aacgttgccg gcgcggggaa tgtcccgcag ccacgacacg
     2221 cacgattggc tgggcggcta tccctacgag tcggccacgg ccgcggaagt gcacgatcgc
     2281 ctcggcgcgc tcggcttcgg catcgtccgc gagaacctca acgaggcctg cctcggcaat
     2341 ctgttcggaa gcacgaacaa cgaatacgtc gcccgccgcc gcacgacggc gaggccgcaa
     2401 tccgccgctg cgggcggcga acgcacgacc ttcggtgctt cctaataaaa cagccgcccg
     2461 attcgttctc gcggcggccg cgctttgccc gctcgcggcg caggccgccg gcggccaaac
     2521 gcagacatcg ggtgccgaag gcttcaccgt tacgccgttc gtcgcgctcg cgacaacgta
     2581 cgatgacaat ctgttcggcg ccgccggcgc gggtgaaggc gacacgatcc tgagcgtcgg
     2641 cccgggtttg gacctgcgct acctgtcccc gcgcacggag tttcgcttct cctacagcct
     2701 cgcggccgcg aaatatctcc atcattccac gctcgactcc tggatcgacg aacagcaggg
     2761 aacgctcggg atcgagcgca agttcaccga tcggctgagc gccggcgtcg acgcgagcta
     2821 cctcgagacg cacgatcccg agcgcctcac cccgacgacg ggactggtgt acggccgcac
     2881 gcgggcgacc gaggaaacgc tccggcc
//
LOCUS       DASUJG010000191         2141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_302745, whole genome shotgun sequence.
ACCESSION   DASUJG010000191 DASUJG010000000
VERSION     DASUJG010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302745"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2141)
                     /locus_tag="VFX38_04010"
     CDS             complement(<1..>2141)
                     /locus_tag="VFX38_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX5314059.1"
                     /translation="VYAEGQRRYVETFSPYARQFLDRQDRPHVERIEGIPPAIAIDQV
                     NPVRTSRSTVGTMSELADHLKLLFARAAALYCTNCGRAVRRDTPEAIAAELLADEALA
                     DGRAMVTFTIEVPRNFSAEEVERFLAQQGYTRIQSRRGKTIEVVQDRLRLNEDNRARL
                     VEALEAAFARGNGRLVVYPLHGGTPHPGPLPSGERGKEVTPLPSGEREMDGRRYSAGL
                     HCAHCDLAYAEPSPALFSFNSPAGACPTCRGFGRTQGIDYGLVIPDESKSLAAGAIKP
                     FQTKSFLECQKDLLRYAKKQGFPVHKPWRDLSEAQKRWVIAGEGGWRDHVWYGVQRYF
                     QWLESKSYRMHIRVLLSRYRAYDTCPDCRGARLKPEALAFRIGAKAEADAVLAPGTRF
                     RPAGTNYDEATLAALPGLTLHDLNTLPISRAREFFAALALPAPLDQASEHLLGAIRGR
                     LDYLATVGVGYLTLDRQSRTLSGGEVQRVNLTTALGTSLVNTLFVLDEPSIGLHPRDI
                     TRITGVLSRLRDAGNTLLVVEHDPAIMLAADRILDLGPGAGNAGGETQYFGPAAGILR
                     APHSATGAWLAGRKTMPVVAAVAPPDAKTPRLVIEGASAHNLKHIDVAIPLGRLVCLT
                     GVSGSGKSTLMEEVLYRALRRAQGKPTETPGTHTRLAGADHFDEVVLVDQSAIGRTTR
                     SNPASYVGAFDAIRKLFAATPLARERGYTLGT"
BASE COUNT          357 a          697 c          720 g          367 t
ORIGIN      
        1 gtgccgagcg tgtagccgcg ttcgcgcgcg agcggcgtcg ccgcgaagag cttgcggatc
       61 gcatcgaagg cgccgacgta actcgccgga ttcgatcgtg tcgtgcgccc gatcgccgat
      121 tgatccacca gcaccacttc gtcgaaatgg tccgctccgg caagtctcgt gtgcgtgccc
      181 ggtgtctcgg tgggtttgcc ctgcgcgcgc cgcagcgcgc gatagagaac ttcttccatc
      241 aaggtggatt tcccgctgcc cgaaacgccg gtaaggcaca cgaggcgacc cagcggaatg
      301 gcaacgtcga tgtgcttgag gttgtgcgcg ctcgcgcctt cgatgacgag ccgaggcgtc
      361 ttcgcatccg gcggcgcgac ggccgcgacg acgggcatgg ttttcctgcc cgcgagccag
      421 gcgccggtcg ccgaatgcgg tgcacgaagg atgccggcag ccgggccgaa gtattgcgtc
      481 tcgccgccgg cgtttcccgc accggggccg agatcgagaa tgcggtcggc ggcgagcatg
      541 atcgcgggat cgtgttcgac caccagcagc gtgttgccgg catcgcgcag gcgcgaaagt
      601 acgcccgtga tgcgtgtgat gtcgcgcgga tgcaggccga tcgagggttc gtcgagcacg
      661 aagagcgtat tcaccagcga cgtgccgagt gccgtggtca gattgacgcg ctgcacctcg
      721 ccgcccgaga gcgtgcgcga ctggcgatcg agcgtgagat agccgacgcc gacggtcgcg
      781 agataatcga gacgcccgcg gattgcgccg agcagatgtt cgctcgcctg atcgagcggc
      841 gccggcaggg cgagcgcggc gaagaattca cgcgcccgtg aaatcggcag cgtgttgagg
      901 tcgtgcagcg tgagtcccgg cagcgccgcg agcgtcgcct cgtcgtaatt cgtgcccgcc
      961 ggtcggaagc gtgtgccggg tgccagcacg gcgtcggctt cggctttcgc gccgatgcga
     1021 aacgcgagcg cttcgggctt caggcgtgcg ccgcggcaat cgggacaagt gtcgtaggca
     1081 cggtagcgcg aaagcagcac gcggatatgc atgcggtagc ttttcgattc cagccattgg
     1141 aagtagcgct ggacgccgta ccagacgtga tcgcgccagc cgccttcgcc cgcgattacc
     1201 caacgtttct gcgcttcgct cagatcgcgc cagggcttgt gaaccgggaa gccctgcttc
     1261 ttcgcatagc gcagcaaatc cttctggcat tcgagaaagg atttggtctg aaacggcttg
     1321 atcgcgcccg cggcgagcga tttcgattcg tcgggaatta cgaggccgta atcaattccc
     1381 tgcgtgcgcc cgaagccgcg gcaggtcggg caggcgccgg cgggcgagtt gaacgaaaac
     1441 agcgccggcg acggttccgc ataggcaaga tcgcagtgcg cgcaatgcaa tcccgccgaa
     1501 taccgccgcc catccatttc cctctccccc gaggggagag gcgtcacctc ttttcccctc
     1561 tccccggagg ggagagggcc agggtgaggg gttcctccgt gcagcggata cacgacgagg
     1621 cgcccgttcc cgcgcgcgaa ggccgcttcc agcgcttcga cgagacgcgc ccggttgtct
     1681 tcgttcaggc gcaagcgatc ctgaaccacc tcgatcgtct tgccgcgccg agattggatg
     1741 cgcgtgtaac cctgctgcgc gagaaagcgc tcgacttcct ccgccgaaaa attgcgcggc
     1801 acctcaatcg tgaacgtcac catcgcgcgc ccgtcggcaa gcgcttcgtc cgccagcagt
     1861 tccgccgcga ttgcctccgg cgtgtcgcgg cgcaccgccc gtccgcaatt cgtgcaatac
     1921 agtgccgccg cccgcgcgaa cagcagcttc agatgatcgg cgagttcgct catcgtgccc
     1981 accgtcgagc gcgaggtgcg caccggattc acctggtcta tcgcgatcgc cggcggaatg
     2041 ccctcgatac gctctacgtg cgggcggtcc tgtcgatcca gaaactggcg ggcgtagggc
     2101 gagaaggtct cgacgtagcg gcgctgcccc tcggcgtaca c
//
LOCUS       DASUJG010000192         5190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1036032, whole genome shotgun sequence.
ACCESSION   DASUJG010000192 DASUJG010000000
VERSION     DASUJG010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036032"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VFX38_04015"
     CDS             <1..479
                     /locus_tag="VFX38_04015"
                     /inference="COORDINATES: protein motif:HMM:NF020416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase/BAAT C-terminal
                     domain-containing protein"
                     /protein_id="HEX5314060.1"
                     /translation="KARSSWTLGGKPLLWVNFVADPAAFAKGGPIAFAPSYAAGLRDS
                     AAVARAAIPVERIGGPVLLVSAGDDQIWPSSVMARHIMQRLRSRRHPYANESLCYAAA
                     GHLILPPYRPTNANATSFAAGSILFGGEPQAYAFADVDAWQKSLAFLARLTTRPGP"
     gene            490..1659
                     /locus_tag="VFX38_04020"
     CDS             490..1659
                     /locus_tag="VFX38_04020"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX5314061.1"
                     /translation="MKQARTTIGTALCAAVLWLHHRLLPADPGVGWVPYLWLIWIGFF
                     FIPWAAQATSTTTMVATVVAIALFLPLYFRYWWVRSTEGPWIIALIAALGCALIPFNS
                     AGGALLIYAGSFAGYSFRPRFTLVVLAALAAVAVGEYVALGLPLIVWIWAPTIILMAG
                     LANMWGAERHRQHCALQRSQDEVKRLATAAERERIGRDLHDLLGHTLTLISVKAELAA
                     RLAERDLPGATKEIRELEAIARDALAQVREAVGGYRAGGIAGEIVSARAALRSADVVF
                     EVDAVAGAATLAEEGVLAMIVREAVTNIVRHAQAHHCVLCVRREEAALVLEISDDGRG
                     GVRTEGNGILGMRERLRELGGSLKIDSGRQGTRLRARIPADATEAAPSALRERLA"
     gene            1656..2258
                     /locus_tag="VFX38_04025"
     CDS             1656..2258
                     /locus_tag="VFX38_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5314062.1"
                     /translation="MIRVLVAEDQSLILGALAALLDMEEDICVIARAENGKLALKAAL
                     AEDPDVVVTDIEMPEMTGLELAQALRDRGARARTIIVTTFARPGYLRRALEAGVRGYL
                     LKDAPARELAAAIRRVHTGGKAIAPELAADAWSDEDPLSERERQVLRLAGEGLTNHAI
                     SERLHLSEGTVRNYLSEAIGKLDAGNRIEAARTARQKGWL"
     gene            2398..2556
                     /locus_tag="VFX38_04030"
     CDS             2398..2556
                     /locus_tag="VFX38_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011327039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HEX5314063.1"
                     /translation="MDLIRIVFSILIPPLGVFLQVGIGLQFWVNVLLTILGYIPGVLH
                     AIYIIVTR"
     gene            complement(2566..4845)
                     /gene="clpA"
                     /locus_tag="VFX38_04035"
     CDS             complement(2566..4845)
                     /gene="clpA"
                     /locus_tag="VFX38_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEX5314064.1"
                     /translation="MLSKELEFCLNSAFKEARDARQEFITVEHLLLALLDVPAVLEAL
                     TACGAEPDVLRETLSGYIEKTTPRLGRQATVDVQPTLGFQRVLQRAVFHVQSSGEKEV
                     SALNVLVALFSEKDSHAVYLLNRLQVSRLDVVNYVSHGITKVGSDAPEEQAGEAESEP
                     KAKKEPSALEQFTRNLNQLARRGKIDPLVGRENELARTLQILCRRRKNNPLFVGEAGV
                     GKTALAEGLALRIVEGRVPELLAGATIYALDLGSLVAGTKYRGDFEKRLKMVLGELAS
                     RPGSILFVDEIHTVIGAGAASGGVMDASNLIKPVLATGELKCIGSTTYQEFRGIFEKD
                     RALARRFQKIDVPEPTVEETVAILDGLKSRFESHHGVRYPARTVRAAAELGARYLNDR
                     RLPDKAIDIIDEAGAEMRLRPLTKRRKIVSVADVETIVARMARIPPKSVSHSDRDALR
                     TLERDLRLSIYGQDAAIAKLAQAIKMARSGLGHPEHPIGAFLFSGPTGVGKTEVTRQL
                     ARLLGIPLVRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTEEVIKNPHAVLLLDEI
                     EKAHQDVFNLLLQVMDHGTLTDNNGRKADFRNVIIVMTTNAGAQEMSRRTMGFMTQDN
                     ASDGMEAIRRIFAPEFRNRLDAIIGFDSLAPETILRIVDKFLIELEAQLEEKHVTLEF
                     REEARRWLAEHGYDPQMGARPMARLIQERIKAPLAEELLFGRLANGGRVVVALRDADL
                     VLDISADAGDRREAAPVAE"
     gene            complement(4848..5168)
                     /gene="clpS"
                     /locus_tag="VFX38_04040"
     CDS             complement(4848..5168)
                     /gene="clpS"
                     /locus_tag="VFX38_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012982662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEX5314065.1"
                     /translation="MSKSRLLETGSGTDVKESRPELKEPPLYKVVLLNDDYTPMEFVV
                     EVLEVFFRLDRSRATQIMLYVHTRGKGIAGVFTHEIAETKAGQVNEYSRHHQHPLLCT
                     LEEA"
BASE COUNT          842 a         1694 c         1703 g          951 t
ORIGIN      
        1 ggaaggcgcg ctcctcctgg acgctcggtg gaaaacctct gctctgggta aacttcgtcg
       61 ccgatccggc cgcattcgcg aagggcggcc cgatcgcctt cgcgccgagc tatgccgcag
      121 gcctgcgcga cagcgccgcc gttgccaggg ccgccattcc cgtcgagcgc atcggcggcc
      181 ccgtccttct cgtttccgcg ggcgacgatc agatctggcc gtcttccgtg atggcgcgcc
      241 acatcatgca gcgtctgcgc tcgcgtcggc atccctacgc aaacgaatcg ctctgctacg
      301 ccgcggccgg ccacttgatc cttccgccgt atcgcccgac caatgcgaat gcgacgagct
      361 tcgccgccgg cagcatcctc ttcggcggcg aaccgcaggc ctacgccttt gccgacgtcg
      421 atgcctggca gaaatcgctc gcgtttctcg cccgcctcac cacccgtccc gggccataga
      481 ataggacgca tgaagcaagc tcgcacgacg atcggaacgg cgctctgtgc ggcggtgctg
      541 tggctgcacc atcggctgct gccggccgat ccgggcgtcg ggtgggtacc gtatctgtgg
      601 ctgatctgga tcggtttttt cttcattccc tgggcggcgc aggcaaccag tacgacaacg
      661 atggttgcca ccgtcgtcgc catcgcgctc tttctgccgc tgtacttccg ttactggtgg
      721 gtgcgcagca ccgaaggacc gtggattatc gcactcatcg cggccctggg ttgcgcgctg
      781 attccgttca acagcgccgg cggcgccctg ctgatctatg ccggcagctt cgccggctac
      841 agctttcgcc cgcgtttcac gctggtggtt ctcgccgcct tggcggcagt cgcggtcggc
      901 gaatacgtcg cgctcggcct gccgctgatc gtgtggatct gggcaccaac catcatcttg
      961 atggccgggc tcgccaacat gtggggtgcc gagcggcatc gtcagcactg cgccctgcaa
     1021 cgctcgcagg acgaggtgaa gcgcctggcc acagccgccg agcgtgaacg cattggtcgc
     1081 gacctgcacg atctgctcgg ccacacgctc acgctcattt ccgtcaaggc cgaactcgcc
     1141 gcccgcctcg cggagcgcga tcttcccgga gccaccaagg aaatccgcga actcgaggcc
     1201 atcgcccgcg acgcgctcgc acaggtgcgc gaggccgtag gcggctatcg cgccggcggc
     1261 atcgcgggcg agatcgtgag cgcgagggcg gcgttgcgtt cggcggacgt tgtgttcgaa
     1321 gtggacgccg tagcgggagc ggcgacgctc gccgaggaag gcgtgctcgc gatgatcgtg
     1381 cgcgaggccg tgacgaacat cgtgcgtcat gcgcaggcgc accattgcgt gctctgcgtg
     1441 cggcgcgagg aggccgccct cgtcctcgaa atctccgacg acgggcgcgg cggagttcgc
     1501 accgagggca acggcattct cggcatgcgc gaacgcctgc gggaactcgg cggcagtctg
     1561 aaaatcgatt ccggacggca aggcacgcga ctgcgtgccc gcattcccgc ggacgcaacc
     1621 gaggccgcgc cgtccgcgct tcgcgagcgc cttgcgtgat tcgggttctc gtcgccgagg
     1681 atcagtcgct cattctcggc gcactcgcgg cgcttctcga catggaagag gacatctgcg
     1741 tgatcgcccg cgcggaaaac ggcaaactgg cgctcaaggc cgcgctcgcc gaagacccgg
     1801 acgtcgtcgt caccgacatc gagatgcccg aaatgacggg cctcgaactc gcgcaggccc
     1861 tgcgcgatcg aggagcgcgc gcgcgtacga tcatcgtcac cactttcgcg cggcccgggt
     1921 atctgcgccg tgcactcgag gcgggagtac gcggttatct tctcaaggac gcgccggcgc
     1981 gggaactcgc cgcggcgatc cgccgcgtgc acacgggcgg caaggcgatc gcgccggaac
     2041 tcgcggccga tgcctggagc gacgaagatc ccctgagcga gcgggaacgc caggtcctgc
     2101 gcctagccgg cgagggcctg accaaccacg cgatctccga acgcctgcat ctttccgaag
     2161 gcacggtgcg caattatctt tcggaggcga ttggcaaact cgacgccggc aaccgcatcg
     2221 aggcggcgcg aacggcgagg cagaaaggct ggctctgaac ctcgcgaatc atcgacgacg
     2281 gagttccgcg ccgaacctgt cacgagccgg ccggatcgct ttcagggaaa tacctcacgg
     2341 ccgcggccca cagcggagcg ttacgatcgg agaaagtatc gcgggagcac taccgtgatg
     2401 gatctgattc gaatcgtttt ctcgattctc atcccgccgc tcggcgtgtt tcttcaggtc
     2461 ggcatcgggt tgcagttctg ggtgaacgtg ctgctcacga tcctcggcta tatcccgggc
     2521 gtgttacacg caatttacat tatcgtgacc cgttagttcc gcggttcatt cggcgacggg
     2581 agcggcctcg cgccgatcgc cggcgtcggc ggaaatgtcg agcaccagat ccgcatctcg
     2641 cagcgccacg acgacgcgcc cgccattggc gagccggccg aagagcagct cctcggcgag
     2701 cggcgccttg atgcgctcct ggatgagccg cgccatcggc cgcgcgccca tctgcggatc
     2761 gtagccgtgt tcggcgagcc agcgacgcgc ctcttcgcgg aactcgaggg tgacgtgctt
     2821 ttcctcgagt tgcgcctcga gttcgatcag gaacttgtcg acgatgcgta gaatcgtttc
     2881 cggcgcgagg ctgtcgaatc cgatgatggc gtcgagacga ttgcgaaact ccggtgcgaa
     2941 gatgcgtcgg atcgcctcca tgccgtcgga ggcgttgtcc tgcgtcatga agcccatcgt
     3001 gcggcggctc atctcctgcg cgccggcatt ggtggtcatc acgatgatga cgttgcggaa
     3061 atcggctttg cggccgttgt tgtccgtaag cgtgccgtga tccatgacct gcagaagaag
     3121 attgaagacg tcctggtgcg ccttctcgat ctcgtcgagc aggagcaccg cgtgcggatt
     3181 cttgatgact tcttcggtaa ggagcccgcc ctggtcgaag ccgacgtagc ccggaggcgc
     3241 gccgatcagg cgcgataccg tgtgccgctc catgtattcg gacatgtcga agcgcaccag
     3301 cggaatcccc aggagccgcg cgagctggcg cgtgacttcg gtcttgccga cgcccgtcgg
     3361 cccggagaag agaaatgcgc cgatcggatg ctccggatgc ccgaggccgg agcgcgccat
     3421 cttgatcgcc tgcgcgagct tcgcgatcgc ggcatcctgg ccgtagatgc tcaggcgcag
     3481 atcgcgctcg agcgtgcgca gggcatcgcg atcggagtgc gagacgcttt tcggcggaat
     3541 gcgcgccatc cgcgcgacga tcgtctcgac gtcggcgacg ctcacgatct tgcgccgttt
     3601 cgtgagcggg cgaaggcgca tttccgctcc ggcctcgtcg atgatgtcga tggccttgtc
     3661 gggaagtcgc cggtcgttca gatagcgtgc gccgagttcg gcggcggccc gcaccgtgcg
     3721 cgccggatag cgcacgccgt gatgcgattc gaagcggctc ttcaggccgt cgagaatagc
     3781 cacggtttcc tccaccgtcg gctccggcac gtcgatcttc tggaagcggc gggcgagcgc
     3841 gcgatccttc tcgaagatgc cgcgaaactc ctggtaggtc gtcgagccga tgcacttcag
     3901 ctcgcccgtc gcgagcaccg gcttgatgag gttggaagca tccatcacgc cgcccgacgc
     3961 cgcgccggca ccgatgacgg tgtgaatctc gtcgacgaag aggatcgagc ccgggcgcga
     4021 ggcgagttcg ccgagcacca tcttcaggcg cttctcgaag tcgccgcggt atttggttcc
     4081 ggccacgagc gagccgagat cgagcgcgta gatcgtcgcc ccggcaagga gttccggcac
     4141 gcggccctcg acgatccgca gcgcgagtcc ctcggcgagc gcggtcttgc cgaccccggc
     4201 ctcgccgacg aagagcgggt tgttcttgcg ccggcggcag agaatctgca gggtacgggc
     4261 gagttcgttc tcgcgcccga ccagcggatc gatcttgccg cggcgcgcga gttgattgag
     4321 gttgcgggtg aactgctcga gtgccgaagg ctcctttttg gctttgggct cgctttcggc
     4381 ttcgcccgcc tgttcctccg gcgcatcgct tccgaccttg gtgatgccgt gggagacgta
     4441 gttgaccacg tcgagacgcg acacctgcag gcggttgaga agatagaccg cgtgcgagtc
     4501 cttctcgctg aatagcgcga ccagcacgtt gagcgccgag acctccttct ctcccgaaga
     4561 ctggacatga aagaccgcgc gctgcagcac gcgctgaaag ccgagcgtcg gctgcacgtc
     4621 cacggtcgcc tggcgaccga gccgcggcgt ggtcttctcg atgtagcccg aaagcgtttc
     4681 gcgcagcaca tcgggttccg ctccgcaggc cgtgagcgct tcgagcacgg cgggcacgtc
     4741 cagaagcgcg agcaggagat gctcgaccgt gatgaattcc tgacgcgcat cgcgcgcctc
     4801 cttgaaggcg ctgttgaggc agaattccag ttccttgctt aacatgctca agcctcctcg
     4861 agggtgcaga gcagcggatg ctgatggtgg cgcgaatact cgttgacctg gcccgccttg
     4921 gtttcggcga tctcgtgagt gaaaactccc gcgatcccct tgccgcgggt gtgaacgtag
     4981 agcatgatct gcgtcgcgcg gctgcgatcg aggcgaaaaa acacctcgag gacctcgacg
     5041 acgaactcca tcggcgtata gtcgtcgttc agaaggacca ccttgtagag cggcggttcc
     5101 ttcaactcgg gacgcgactc cttgacgtcg gttcccgatc cggtttcgag caggcgtgat
     5161 ttggacatga ttcgattgta gctcatgcgt
//
LOCUS       DASUJG010000193         2648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_180292, whole genome shotgun sequence.
ACCESSION   DASUJG010000193 DASUJG010000000
VERSION     DASUJG010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180292"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..688)
                     /locus_tag="VFX38_04045"
     CDS             complement(200..688)
                     /locus_tag="VFX38_04045"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEX5314066.1"
                     /translation="MDAILRARLRQWFAPPRSRDEQGENIELAVAALLAEMIRADYQS
                     DAGERETAGQMLERHFDIDEAAAARLLAEGERAADRAVSLFEHTRALDVGLDPEEKFA
                     VIEALWEIAFADGSLDGKEDYLVHKIGALLHLRHSDLMRLKESVRRRSGESQPQGSAD
                     DT"
     gene            complement(688..1362)
                     /locus_tag="VFX38_04050"
     CDS             complement(688..1362)
                     /locus_tag="VFX38_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003094302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5314067.1"
                     /translation="MNRARLERENLVIPGPAGEIEALLERRAHAEPRAIAVICHPHPQ
                     FRGSLDNKVVYTLSRAASGAGAAALRFNFRGVGQSAGEYDGGRGESEDLAAAERWLAA
                     RCPGAPLWRMGFSFGAAMAIKASIGEPCAILVTVAPPVASLEDYGVREGASPQASRWL
                     LVQGERDDVVSPEAVFAWSERRRPTPEVARFADAGHFFHGRLTELRERVLAFLESASG
                     AGGEIG"
     gene            1666..1980
                     /locus_tag="VFX38_04055"
     CDS             1666..1980
                     /locus_tag="VFX38_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314068.1"
                     /translation="MSITTLRKGLAVSLAMAAFALLGACVVEPTPQPQSQLVLTAAPT
                     TGTCTSCGVITSVQTVEPADYRVTIRMDTGDMRTLDQAQQPAFQVGDRVQIVTRTQYP
                     SG"
     gene            complement(2023..>2648)
                     /locus_tag="VFX38_04060"
     CDS             complement(2023..>2648)
                     /locus_tag="VFX38_04060"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX5314069.1"
                     /translation="TRLLVVGASLWSSAFARALIDAGGEVLVVDNVWRRLRAPRLAGV
                     PVYYGEILSDRAEAALELTDVGSLLAAGANDAYNALVCASFAADLGRENVFQLPLHGG
                     EDGDPRGLTRPRRGRIAIAAQAHHEWLEERLGEGWRFQKTQFSETFTYQDFLAQASGE
                     GVAVALRLADGSLFLAASDESRHPRAGDVLLSFAPPKPLAAGAEAQT"
BASE COUNT          446 a          914 c          878 g          410 t
ORIGIN      
        1 gaaagaaatg gcctgagggg cctccgttcc ccggggaacg gcaaggaggg ggcgagtccg
       61 ggccgctccg gactcgcttc cacgccgccg tcgcggccga tccgcagccg gcggacaaag
      121 ttcgcgttca tcgaagcgcg aacgacgcag ttccgcgcga tgacatgaac gagaaccggc
      181 ccgcgggacg cggcgggcct tacgtgtcgt cggccgatcc ctggggttgc gactccccgg
      241 agcgtcggcg cacggactcc ttcaggcgca tgagatcgga atggcgcagg tgcagcagcg
      301 cgccgatctt gtgcaccaga taatcctcct tcccatcgag gcttccgtcg gcgaaggcga
      361 tttcccatag cgcctcgatt accgcgaatt tttcttcggg atcgaggccg acgtcgagcg
      421 cccgcgtatg ctcgaagagc gacaccgcgc gatctgctgc gcgctcccct tcggcaagaa
      481 gccgggccgc ggccgcctcg tcgatgtcga agtgccgctc gagcatctgc cccgcggttt
      541 cccgctctcc cgcatcgctc tggtaatcgg cccggatcat ctcggccagc agtgccgcga
      601 cggccagttc gatgttttcc ccttgctcgt cgcgggagcg gggcggcgcg aaccattgac
      661 ggaggcgggc gcgcaatatt gcatccatca gccgatctcc ccgccagccc cgcttgcgga
      721 ttcgagaaat gcgagcaccc tttcgcgcag ttcggtgagc cgcccgtgaa agaagtggcc
      781 ggcatcggcg aagcgcgcga cctcgggcgt cggccggcgg cgctcgctcc aggcgaacac
      841 cgcttcgggt gaaacgacgt cgtcgcgttc tccctgcacc agcaaccaac gtgatgcctg
      901 cggcgatgct ccttcccgca cgccgtaatc ctcgagactc gcgacgggcg gagcgacggt
      961 cacgagaatc gcgcagggct cgccaatgct ggccttgatc gccatcgccg cgccgaagga
     1021 aaatcccatg cgccagagtg gcgctcccgg gcagcgggcc gcgagccagc gctccgcagc
     1081 ggcgagatcc tcgctctcgc cgcgcccgcc gtcatattcc ccggcgctct ggcccacacc
     1141 gcgaaagttg aagcggaggg cggcggctcc ggcaccggac gcagcccgcg aaagcgtgta
     1201 cacgaccttg ttgtcgaggc tgccgcgaaa ctgcggatgg ggatgacaga tgacggcgat
     1261 cgcgcgcggc tcggcatggg ccctgcgttc gagcagggcc tcgatttcgc ccgcagggcc
     1321 gggaatgacc aagttttcgc gttcgagcct cgcgcgattc attcgattcc tgacggtgtg
     1381 atggcggtgc cgcagccccc gagtccaggc gcgaatctcg ctcccgcggc ttggagtcgc
     1441 tcgggagcgg ggcagcagaa ccgccgatgg acccgctccc gtccgtgtcg gacgcgctcg
     1501 caggtcgatg acgatcattt tagcggacgc aatcacctga atcgtgcgga gccgcggaac
     1561 ccgaatcgcc ggcctgcact cgaaaaccaa ggaggagttt gcagcggcga cggcatccgc
     1621 gccgtgggga acgttgcgac tcaacaatcg aaggagaagt tgtccatgag catcacaaca
     1681 ctacgcaaag gcctcgccgt cagcctcgcc atggcggcgt tcgcgctgct gggggcctgc
     1741 gtcgtcgagc cgactccaca gccccaatcc caattggttc ttaccgcggc cccgacgacc
     1801 gggacctgca ccagttgcgg cgtgatcact tcggtccaga cggtcgagcc cgccgactac
     1861 cgggtcacga tccgcatgga tacgggagac atgcgcacgc tggatcaggc tcagcagccg
     1921 gcgttccagg tcggtgatcg cgtgcagatc gtcacccgca cccaatatcc ctcgggctga
     1981 gctccgggtc ccgcggatcg cgatgcgatc ccgcgaagcg gctcaggtct gcgcctccgc
     2041 gccggcagcc aagggtttcg gcggcgcgaa ggacagcagc acgtcgcccg ctcgtgggtg
     2101 acgcgattcg tcgctcgcgg cgaggaaaag cgacccgtcg gccagacgca gcgcgacggc
     2161 gacaccctcg ccgctcgcct gcgccagaaa atcctggtag gtgaaggttt cggagaattg
     2221 cgttttctga aatctccagc cctcgccgag ccgttcctcc agccattcgt gatgcgcctg
     2281 cgccgcgatg gcgatgcggc cgcgacgggg tcgcgtcagg ccgcgagggt cgccgtcctc
     2341 gcccccgtgc agcggcagct ggaacacgtt ctcgcgcccc agatcggcgg cgaagctcgc
     2401 acatacgagg gcgttgtacg catcgttggc gcccgccgcg agaaggcttc ccacgtcggt
     2461 gagttcgagc gcggcctcgg cgcgatcgga gagaatttcc ccgtaataga cggggacgcc
     2521 cgcgagccgg ggcgcgcgca ggcgtcgcca gacgttgtcg accacgagca cctcgccgcc
     2581 ggcgtcgatc aaagcccgcg cgaatgcact cgaccagagc gaggcaccga ccacgagaag
     2641 gcgcgtgc
//
LOCUS       DASUJG010000194         2322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1037625, whole genome shotgun sequence.
ACCESSION   DASUJG010000194 DASUJG010000000
VERSION     DASUJG010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037625"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..>2322
                     /locus_tag="VFX38_04065"
     CDS             38..>2322
                     /locus_tag="VFX38_04065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5314070.1"
                     /translation="MCVFAAHSAIRDPPFSKLDLVSCRNLLIYMTNDLQDRVVRRFHY
                     ALKPGGWLFLGASEGVSRNSRLFSTVDKKHRLFRRVETEEATLPDVEPRMPRVGTPTP
                     AEPRPEQARGGGDALDRRARRLMEKYAVPYVIVDGRREILRFSGGTINQYLEPSAGNA
                     SLEVLGMLRKALRPTVRTALQQAFENGEAIVHESVPLRIDGKARAVTVIVEPLDSPGN
                     DGIPDCVVAFQDGGPARGSAEARAGHDGGVAAALEHELRVTKAQLNSAIADLETANEE
                     MRSANEEYQSVNEELQSSNEELETSKEEMQSINEELQTVNVELGHKNESLGAANSDLR
                     NLLESTQIATLFLDSRLCIRGFTPGIQEIFHLRDSDRGRPVTDIVTRLNYQALRRDAE
                     KVLRDLAVIEHEVQVTDGEGETFIMRIRPYRTVDNVIDGVVITFTDISERKRSEEAQA
                     RLAALVNASYDAIIGHSLDGRIVFWNSAAERIFGYSASEAIGKPMTMLIPEEQGDEVP
                     ALIERLQRGESVPKYDVIRETRDGQRIDLELSISPTPDQKGKMIAAATIARDISTRLE
                     HEAHRELLMNELSHRVKNTLAIVQSIMMQTIANAPSLDAFGKIFAERIAALATAHDLL
                     MRSNWRGVRLDDLIAAELKPYFVSGKECWRLDGPAVEIAPNQAIALAMAFHELATNAA
                     KYGALSAAEGCVEVQWRLEAREAARILHIDWAERQGPPVVEPKRYGFGMRLVREGLSY
                     ELEAKVNLDFAPNGLHGTIAFP"
BASE COUNT          459 a          743 c          748 g          372 t
ORIGIN      
        1 ggaaggcgat taccgtccgg caaaggaaat ccgttcgatg tgcgtcttcg ccgcccacag
       61 cgcgatcagg gacccgccgt tctcgaaact cgatctcgtc tcctgccgca atcttctgat
      121 ctacatgacg aacgatttgc aggatcgcgt ggtgaggaga ttccactatg cgctgaagcc
      181 gggaggctgg ctgtttctcg gggcttcgga gggagtttcg cgcaactccc ggctgttctc
      241 gacggtggac aagaagcacc gccttttccg tcgcgtcgag acggaagaag ccacgcttcc
      301 cgatgtcgag ccgcgcatgc cgcgggtggg gacgcccacg ccggccgaac cgcggcccga
      361 gcaggcgcgc ggcgggggcg atgcgctcga tcggcgggcg cgccgcctca tggaaaagta
      421 cgcggtgccc tatgtgatcg tggacgggcg ccgcgagatc ctgcgatttt ccggcggcac
      481 gatcaatcag tatctcgaac cgtcggcggg taacgcgagc ctcgaagttc tcggcatgct
      541 gcgcaaggct cttcgcccga cggtgcgcac ggccctgcag caggccttcg agaacggcga
      601 ggcgatcgtg cacgagagtg tgccgctgcg catcgacggc aaggcgcggg ccgtgacggt
      661 gatcgtcgaa ccgctcgatt cccccggaaa cgatggcatc ccggactgcg tggtcgcttt
      721 ccaggacggc ggcccggccc gcggcagcgc ggaggctcgg gccggccatg acggcggcgt
      781 cgccgccgca ctggaacacg aactgcgcgt caccaaggcc cagctcaatt cggcgatcgc
      841 ggatcttgaa accgccaacg aggaaatgcg ttccgcgaac gaagaatacc agtcggtgaa
      901 cgaggagttg caatcctcga acgaggaact cgagacttcc aaggaagaga tgcagtcgat
      961 caacgaagag ctgcaaacgg tcaacgtcga actcggccac aagaacgaat cgctgggcgc
     1021 ggccaacagc gatctgcgca accttctgga gagtacccag atcgccacgc tctttctcga
     1081 ctcgcgcctg tgcatccgcg gtttcacgcc cggaattcag gagatctttc atctgcgcga
     1141 cagcgatcgc gggcgccccg tcaccgacat cgtgacgcgg ctcaactatc aggccctgcg
     1201 ccgggatgcg gagaaggtgc tgcgcgatct cgcggtcatt gaacacgagg tccaggtgac
     1261 ggacggcgaa ggcgagactt tcatcatgcg catccggccc tatcgcacgg tggacaacgt
     1321 catcgacggg gtggtcatca ccttcaccga catcagcgag cgcaagcgca gcgaagaggc
     1381 ccaggcccgg ctcgcggcgc tggtgaacgc ctcctacgat gcgatcatcg gacattcgct
     1441 ggacggacgc atcgtcttct ggaattccgc ggcggaacgc atcttcggct acagcgcgag
     1501 cgaggccatc ggcaagccca tgacgatgct gattcccgag gagcagggcg acgaagttcc
     1561 ggcgctcatc gagcggctgc aacgcgggga gtcggtgccc aaatacgacg tcattcgcga
     1621 gacgcgcgac gggcagcgaa tcgatctcga attgagcatt tcgccgacgc ccgatcaaaa
     1681 aggcaagatg atcgcggcgg cgaccattgc ccgcgacatc tccacgcgcc tcgaacacga
     1741 agcgcaccgc gaactgctga tgaacgaact gagccatcgc gtcaaaaaca cgctcgcgat
     1801 cgtgcagtcc atcatgatgc agacgatcgc gaatgcgccg agcctcgatg ccttcggcaa
     1861 gatcttcgcc gagcgcatcg ccgcgctcgc gaccgcgcac gatcttctca tgcgcagcaa
     1921 ctggcgcggc gtgcggcttg acgatctcat cgccgcggaa ctcaagccct atttcgtctc
     1981 cggaaaggag tgctggcggc tcgacgggcc cgcggtcgag atcgcgccca atcaggcgat
     2041 cgcgctggcg atggcctttc acgaactcgc gaccaacgcg gcgaagtacg gcgcgctttc
     2101 cgcggcggag ggatgcgtcg aggtgcagtg gcgactcgag gcgcgcgaag ccgcccgcat
     2161 ccttcatatc gactgggccg agcggcaagg gccgccggtc gtggaaccca agcgctacgg
     2221 cttcggaatg cgcctcgtgc gcgaaggact gagctacgaa ctcgaggcga aggtgaatct
     2281 cgacttcgct ccgaacggac tgcatggcac gatcgccttc cc
//
LOCUS       DASUJG010000195         2975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_425833, whole genome shotgun sequence.
ACCESSION   DASUJG010000195 DASUJG010000000
VERSION     DASUJG010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425833"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1687
                     /locus_tag="VFX38_04070"
     CDS             <1..1687
                     /locus_tag="VFX38_04070"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX5314071.1"
                     /translation="DEGLRLVEREPGGDDAHERTLANILGAYDEACERAGLVDFAELL
                     LRSTRLFLEHGEIAAQYRMRFRHILVDEFQDTNTVQYLWLRAIAGDDIRPFIVADDDQ
                     SIYGWRGARVGNLKRFQHDYAPVTILRLEQNYRSTGNILAAANAVIAKNRERLGKTLW
                     TAGAQGAPIELYAASDERDEAQHLVARIERWIADGGSVEDAAVLYRSNAQSRVFEETL
                     IARGIPYRVYGGLRFFERAEIKDALAYLRLALRREDDAAFERIVNVPARGIGAQTLAA
                     IRTHARARRLSLWDASRELAAGGSLAGRARSALAGFAVLIERIGAQTGEGRLGETVEF
                     AIAASGLAEHYRSQGSEGGEMRVENLAELVSAANEFAADPDLDPLAAFLAQAALEAGD
                     TQAGEWTRAAKLMTLHSAKGLEFPLVLICGLEEGLFPHQKSVETPAGIEEERRLFYVG
                     VTRAREQLLLSYAERRHLHGQVYYSRPSRFLAELPEDGIEETGPRRLARAAGDEAASA
                     ALELGRRVHHPVFGEGVVLEQAGDGAHLRIKVAFENGGAKWLVAAFARLEVLA"
     gene            1866..>2975
                     /locus_tag="VFX38_04075"
     CDS             1866..>2975
                     /locus_tag="VFX38_04075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter"
                     /protein_id="HEX5314072.1"
                     /translation="MNGVGTTILYTFAVYMAVVLLISFVAWARNRSLKDYILAGRGLG
                     NFTTALSAEASDMSGWLLLALPGAAYAFGLGEMWMVIGLIVGTYINWLVVARKLREET
                     QHYGDALTLPDYFAGRFGDRTHLLRGISALFILVFFVFYTSSGFVAGADLFQRLFGVP
                     YFWALVYGTLIVVSYTFIGGFLAVAWNDVLQGSIMFFALVVVGLLGLAYVGWFHGLGS
                     TLNAVNAGLLHPFYDVQHGRAFGWIAIVSLLAWGLGYFGQPHILARFMAIKDSSGIPT
                     ARRIAMFWVIIALVAAMIVGMTGLGVVNHPLAGDDRERVFIFMSLQLLPAVLAGIALA
                     GILAAIMSTASAQLLVAASAFAEDLYHAFFRRRAGR"
BASE COUNT          453 a         1025 c          974 g          523 t
ORIGIN      
        1 ggacgagggg ctgcggctcg tcgaacgcga gcccggcggc gatgacgccc acgaacgcac
       61 gctcgcgaac atcctcggcg cctatgacga ggcctgcgag cgcgccgggc tggtggattt
      121 cgccgagctg ctgctgcgca gcacgcgtct cttcctggag cacggcgaga tcgcggccca
      181 gtaccgcatg cgcttccgcc acattctggt ggacgagttc caggacacca acacggtgca
      241 gtatctgtgg ttgcgcgcga tcgcgggcga tgacatccgg cccttcatcg tcgccgacga
      301 cgatcagtcg atctacggct ggagaggcgc gcgggtcggc aatctcaagc gctttcaaca
      361 tgactacgcg ccggtcacga tcctgcgcct ggagcagaat taccgctcga ccggcaacat
      421 tctcgccgcc gccaacgcgg tgatcgcgaa gaatcgcgaa cgcctcggga aaacgctgtg
      481 gacggcgggg gcgcagggcg cgccgatcga gctctacgcg gccagcgacg agcgcgacga
      541 ggcgcagcat ctcgtcgcac gcatcgagcg ctggatcgcc gacggcggca gcgtcgagga
      601 tgcggccgtg ctctatcgct cgaacgcgca atcgcgcgtc ttcgaggaga cgctcatcgc
      661 ccgcggcatt ccgtatcgcg tctacggcgg cctgcgattt ttcgagcgtg ccgaaatcaa
      721 ggacgcgctc gcgtatctcc ggctcgcgct ccgccgcgag gacgacgccg ccttcgagcg
      781 gatcgtgaac gttcccgccc gcggcatcgg cgcgcagacg ctcgcggcaa tccgcacgca
      841 tgcgcgcgcg cggcggctct cgctctggga tgcgagccgc gaactcgcgg ccggcggcag
      901 tctcgccgga cgggcgcgaa gcgcgctcgc gggattcgcc gtgctgatcg agcggatcgg
      961 cgcgcagacc ggcgagggca ggctcggcga gacggtggag ttcgcgatcg cggcgagcgg
     1021 gctcgcggag cattaccgct cccagggaag cgagggcggc gaaatgcgtg tcgaaaatct
     1081 tgccgaactc gtgagcgcgg cgaacgaatt cgccgccgat cccgacctcg acccgctcgc
     1141 ggcctttctc gcgcaggccg cgctcgaggc cggcgacacg caggccggcg agtggacgcg
     1201 cgcggcgaaa ctcatgacgc tgcactcggc gaagggactc gagtttccgc tggtcctgat
     1261 ctgcggcctc gaagaaggac tctttcctca tcagaagagc gtcgaaacgc cggccggaat
     1321 cgaggaagag cggcggcttt tttacgtcgg agtcacccgc gcacgcgaac aactgctgct
     1381 gtcctacgcc gagcgccggc accttcacgg tcaggtctat tactcgcgcc cgtcgcgctt
     1441 cctcgccgaa ttgccggagg acggaatcga agagaccggt ccgcggcgcc tcgcgcgcgc
     1501 cgccggcgac gaggcggcga gcgctgcgct cgaactcggc cggcgggtgc atcatccagt
     1561 gttcggcgag ggggtggtgc tggagcaggc cggcgacggc gcgcatctgc gcatcaaggt
     1621 cgccttcgaa aacggcggcg ccaagtggct ggtcgcggcc ttcgcgcgcc tcgaggtgtt
     1681 ggcgtaggga agcaagtcgc cagccgactg caagaaagca agcggcaagt caagcgtcag
     1741 cgctgacatc ctcacaggcc gcgaagcggc cgactggcag cgagcggcag caagcgcctc
     1801 gccgcccggc cccccactgc ggtatctgtc agaatgagcg cctttcgcgc tggcgggaag
     1861 gcggaatgaa cggcgtcgga acgaccattc tctacacctt cgcggtctac atggccgtgg
     1921 tgcttctcat ctccttcgtc gcctgggcac gcaaccgcag cctgaaggac tacatcctcg
     1981 cgggacgcgg gctcggcaac ttcaccaccg cgctctccgc tgaagcctcc gacatgtccg
     2041 gctggctgct ccttgcgctg ccgggcgccg cctacgcctt cgggctcggc gaaatgtgga
     2101 tggtgatcgg cctcatcgtc ggcacctaca tcaactggct cgtggtcgca cgcaagctgc
     2161 gcgaagaaac gcagcattac ggcgatgcgc tcacccttcc cgattatttc gccgggcgtt
     2221 tcggcgaccg cacccatctc ttgcgcggaa tctcggcgct cttcattctc gtcttcttcg
     2281 tcttctacac cagttccggt ttcgtcgccg gcgcagatct ctttcagcgc ctcttcggcg
     2341 tgccctactt ctgggcgctc gtgtacggca ccctgatcgt cgtctcctac accttcatcg
     2401 gcggctttct cgccgtcgcc tggaacgacg tgctccaggg ttccatcatg ttcttcgcgc
     2461 tcgtggtcgt ggggctgctc ggccttgcgt atgtcggctg gttccacggc ctcggatcga
     2521 cgctcaacgc ggtgaacgcg ggcctgctcc atccttttta cgacgtgcag cacgggcggg
     2581 ccttcggctg gatcgcgatc gtgtcgctcc tcgcctgggg actcggttac ttcggccagc
     2641 cgcacattct cgcgcgcttc atggcgatca aggactcgag tggcatcccc accgcgcggc
     2701 gcatcgccat gttctgggtc atcatcgccc tcgtggcggc gatgatcgtc ggcatgaccg
     2761 gcctcggcgt ggtcaaccat ccgctcgccg gcgacgatcg cgagagggtc ttcatcttca
     2821 tgtccctgca actgctgccg gcggtgctcg ccggcatcgc gctcgccggc attctcgccg
     2881 cgattatgtc caccgcctcg gcccagcttc tggtggccgc ctcggccttt gccgaagacc
     2941 tctatcacgc cttcttccgc cgccgggcgg gacgg
//
LOCUS       DASUJG010000196         6906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1770760, whole genome shotgun sequence.
ACCESSION   DASUJG010000196 DASUJG010000000
VERSION     DASUJG010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6906
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1770760"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VFX38_04080"
     CDS             <1..184
                     /locus_tag="VFX38_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015095971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidiltransferase"
                     /protein_id="HEX5314073.1"
                     /translation="NDWAGKFDDLTDQCDVVYLPRTEGISTSALKEALRVVDRSHIGD
                     LKKALDLISSIVQRFE"
     gene            complement(207..1334)
                     /gene="wecB"
                     /locus_tag="VFX38_04085"
     CDS             complement(207..1334)
                     /gene="wecB"
                     /locus_tag="VFX38_04085"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003138472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEX5314074.1"
                     /translation="MSRAKKRILLVFGTRPEAIKMAPVVSALAAERERAELRVCVTAQ
                     HRQMLDQVLALFAIAPERDLDLMMTDQGLAELTARSLCALQAMLREWRPDVVVVQGDT
                     TTTFSAALAAFYERIDVAHVEAGLRTGNRYSPWPEEMNRRLTSALATHHFAPTERARD
                     NLLAEGIPGERIRVTGNTVIDALLRIVALARGEAATELARRFSYLDATRRLIVVTGHR
                     RENFGPGFERICRALRRLAQRPDVEIVYPVHLNPNVRAPVGRLLDGVANIHLVEPLDY
                     LPFVGLLDRSWIILTDSGGVQEEAPSLGKPVLVLRDTTERPEAVAAGTVRLVGTDEDR
                     IVAEAARLLDEPECYEAMARAHNPYGDGKAAPRIVKELLDA"
     gene            1633..4437
                     /locus_tag="VFX38_04090"
     CDS             1633..4437
                     /locus_tag="VFX38_04090"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314075.1"
                     /translation="MPNLRPRVPVLVSVLGPLAIAVALAAWLPAGSPRGTGAEILNAG
                     QRARLARLDVPFVANAGQTDPRVAYYAHTMAGTAFVTRSGELVLSLAGRDRRPIPSDT
                     GASAGGAPGWVLVARPVTRQQPQIRGEERTRARVTVLRGSDPKHWGSRLPGYRAITLG
                     QPWAGIGYTLQAHGANVEQLFTVAPGASAGAIRMAISGARALRLKDGALVAETGLGPV
                     RFSRPRAFQLVAGQRRAIAVAYVLAGDRYGFRLGRHDPTRAVVIDPLIRASYLGGSSF
                     DSAAGAVVAPDGDVYVAGQTQSADFPGTTGGAQPQKRGLTDAFIAEFNPSLTTLLHAT
                     YLGGSNLDYATAIALGPPGTPLAGDVYVIGDTRSEDFPGTSGGAQPTCAGTTEDCNDN
                     GDGFVAVLSGDLGTLLQASYLGGTDIDDARALALAPAGAGDPGAVYVAGRTISDDFPA
                     TGGGAQPIAQPGSYGGFIAALSPDLTEIVQATYLGGTSVDEALAVAYDSENNEIYAAG
                     DTSSADFPCTLAGAPPPAGGSCGSSGSGAQSSYAGAFDAFVAALNADLTGLIQSSYVG
                     GSALDQALTLATASGGIYIAGQTYSSDLPAISGAAQPLYDSNGDGFLAKVNPALTAFV
                     RTTYLGGSGLDAADALALGPAGQVYVAGSTQSTDFPCTASSGPDPNGGVCAAAHAGAQ
                     PNAGGGGDAYSALLSADLGRLTQASYLGGGAPDQGNALALAPASGSVSGNVYLVGLTN
                     SVNFPGTAGAAQPAYAGSSDAYAAVLSPDLTGPQLVMTLAVTAPATARRNTFFTYNVD
                     ITNTTAPSAEFDGNATGVVFTDVLPPQVTVKKMTSSQGDDCTQLSGVVSCNLGTIAAN
                     GGDATVTVKVLATNSGSAANAAFIRADQALSEDSASSVTTTTSIPFPNSGNGGASGFG
                     FAGLLALAVLGLFGYLLRRR"
     gene            4434..4895
                     /gene="nudB"
                     /locus_tag="VFX38_04095"
     CDS             4434..4895
                     /gene="nudB"
                     /locus_tag="VFX38_04095"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEX5314076.1"
                     /translation="MTNGASGSRFRFRRPESVLVLVAVVAGPVLLLERRTPPGLWQSV
                     TGSLRWGETAAAAAARELAEETGIEAAGRIEDLGSGARFRILAAWRHRFAPGVVDNDE
                     HWFRLWLDRPVAVRLAPREHGRFAWVSPTQAAARVFSWSNRAAIERYLARD"
     gene            complement(4877..5977)
                     /locus_tag="VFX38_04100"
     CDS             complement(4877..5977)
                     /locus_tag="VFX38_04100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5314077.1"
                     /translation="MIVIELCLSVGRGGLERYAGGLGPQLAARGVDAKAVARRGSEFA
                     RLAQAVAALEINSGRLSPWGNFPALTRLAREADVLHIHRSADLPLAVLVKRAAAGHPA
                     LVYTRHMAISRDRRKSPVHRWLHREVDLFLAVTARVAAEARERLPLPPERIRVLPLGI
                     SDPPPPGDCGELRPPGTGFLIGCFSRIERAKGQQELIAALATLVGRGIDASLVIAGPV
                     MDEVYAEELRASARRLGIAERLRFAGVLADARPAMACCDVVVMPSAAETFGLVLVEAL
                     QMRVPVVCTAAGGTAEIVRDGETGLSYAPGDVSALADRLERLAGDAPFARRLAQAGHD
                     DVVAQFGLETHLERLEELFRSVAPRGRQSRAR"
     gene            6022..6705
                     /locus_tag="VFX38_04105"
     CDS             6022..6705
                     /locus_tag="VFX38_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5314078.1"
                     /translation="MQAATPTPIIETTTPRVVFVHGLFMSGREGVLLSRRLRRQALPC
                     ERFVYSSLRDNPEEAAARLASRLRAEPALHLIGHSLGAVIVAMAIAAVPEWRGRAVLL
                     GPPLAGSVTARRAAVLPGGRRLLGSGGRFLLGELHFGRSSRRVLVIAGVRNFGVGRLI
                     GSCPGPGDGQVRLVETRLAGAAHRSVRRGHMALLFDRRVARAVASFFRDGNPPGATLP
                     APSPGYRRL"
     gene            complement(6721..>6906)
                     /locus_tag="VFX38_04110"
     CDS             complement(6721..>6906)
                     /locus_tag="VFX38_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314079.1"
                     /translation="LAELEANRPHASGQGQLFAPETSAPQPDPLHARLAALDPDALSA
                     RDALALLYELKKLADGN"
BASE COUNT         1011 a         2406 c         2369 g         1120 t
ORIGIN      
        1 gaacgattgg gccggaaagt tcgatgattt gaccgatcag tgcgacgtgg tttatctgcc
       61 ccggaccgaa ggcatttcca cgagcgccct gaaagaggcc ctgcgcgtgg tagatcggtc
      121 acacatcgga gacttgaaaa aggccttgga cctgatttcc tcgatcgtgc aacgattcga
      181 gtaggcacgg gttcgttccg atccgctcag gcatcgagca gctccttcac gattcgcggc
      241 gcggccttgc cgtcgccgta gggattgtgc gcccgcgcca tcgcctcgta gcattccggc
      301 tcgtcgagga gacgcgcggc ctcggcgacg atccgatcct cgtccgttcc gacgaggcgc
      361 acggtgccgg ccgcgaccgc ctcggggcgc tccgtggtat cgcgcaggac gagcacgggc
      421 ttgccgagcg agggcgcctc ctcctgcacg ccgccggaat cggtgagaat aatccaggag
      481 cggtcgagga ggccgacgaa gggcagataa tcgagcggct ccaccagatg aatgttggcg
      541 acgccgtcga gaagccgccc gacgggagca cggacgttcg gattgagatg cacgggatag
      601 acgatttcga catcggggcg ttgcgcgagg cgccgcaggg cgcgacagat gcgctcgaat
      661 ccgggcccga agttctcgcg ccggtgtccg gtcacgacga tcagccgccg cgtcgcatcg
      721 agataggaaa agcgccgtgc cagttccgtc gccgcctcgc cgcgggcgag tgcgacgatc
      781 cgcagcagcg catcgatcac cgtgttgccc gtcacccgga tccgttcgcc cgggatgcct
      841 tcggcgagaa ggttgtcgcg ggcgcgttcg gtgggcgcga agtgatgcgt cgcgagcgcg
      901 ctcgtcaggc gccggttcat ctcctccggc cagggagaat agcggttgcc ggtgcgcaag
      961 ccggcctcga cgtgggcaac gtcgatgcgt tcgtaaaacg cggcgagcgc cgcgctgaac
     1021 gtggtggtgg tgtcgccctg caccacgact acatccgggc gccactcgcg cagcatcgcc
     1081 tgcaaggcgc agagactgcg cgcggtcaac tcggcaagcc cctggtcggt catcatcagg
     1141 tcgagatcgc gctcgggcgc gatcgcgaag agcgcgagaa cctgatcgag catctggcgg
     1201 tgctgcgccg tcacgcatac gcgcagttcc gcacgttcgc gctcggccgc gagcgcgctc
     1261 acgaccgggg ccatcttgat ggcctcgggg cgggtgccga aaacgagcag gattcgcttc
     1321 ttcgccctgc tcatcgggac gcgccatcgg cgcgccgtcg cgccgttccc gcagtccttc
     1381 tcgccggagg tgaatgcatc atcacgaccg tgacatgcgt ggtgccagcg ctacagtgta
     1441 gcggcccccg gattccgccc gagcctgcgg gtgcggccgg gggaaaggcg cgagcgaccc
     1501 tgcactgacc atggacgccg ctcctcatac cctagaatag ggctccgcgc ggcggcacct
     1561 gcgatgtcgg cgccaacgga gggaacccgc cgggtcggcg ttcctccaag tctgaacaga
     1621 ggtcgcttcc tgatgcccaa cctgcgccca cgtgttcccg ttcttgtctc ggtcctgggg
     1681 ccgctcgcca tcgccgtggc tctggcggcg tggctgcctg cgggcagccc gcgcgggacc
     1741 ggggccgaga tcctgaacgc cggccagcgc gcgcggctcg cgcggctgga tgtccccttc
     1801 gtggccaacg ccggtcagac cgacccgcgg gtagcctatt acgcgcatac gatggcgggt
     1861 acggcgttcg taacccgctc cggcgagctg gtcctgtcac ttgccggcag ggatcggcgg
     1921 ccgatcccct ccgacaccgg cgcttccgca ggtggcgctc cgggttgggt tctcgttgcc
     1981 agacccgtca cccggcaaca gccgcagatc cgcggcgagg agcggacgcg ggctcgcgtc
     2041 acggtccttc gcggcagcga cccgaagcat tgggggagcc gcctgcccgg ctatcgcgcc
     2101 attaccctcg gccagccctg ggcgggaatc ggctatacgt tgcaggcgca cggggcgaac
     2161 gtggaacagt tgttcaccgt cgctcccggc gcgagcgcgg gcgcgattcg catggcgatc
     2221 tccggtgccc gcgcgctccg cctgaaggat ggggccctgg tcgccgagac gggcctcggg
     2281 ccggtacgct tctcgcgccc gcgggcgttt cagctggtcg cggggcagcg acgtgcgatt
     2341 gccgttgcct atgtgctcgc cggcgaccgc tatggcttcc ggctgggccg gcacgacccg
     2401 acgcgggcgg tcgtgatcga tccgctgatt cgcgcgagct atctcggcgg ttcctcgttc
     2461 gattccgcgg ccggcgccgt cgtcgcgccc gacggtgacg tgtacgtggc cgggcagacg
     2521 caatcggcgg atttccccgg gacgacgggc ggggcacagc cgcagaagcg cggtctcacg
     2581 gacgccttca tcgccgaatt caacccttcg ctcacgacgc tgctgcatgc gacctacctc
     2641 ggaggcagca atctcgatta cgcgaccgcg atcgccctcg gcccacccgg gacgccgctc
     2701 gcgggcgacg tttacgttat cggcgacacc cgctcggaag atttccccgg cacttcgggc
     2761 ggcgcccaac cgacctgtgc cggcacgacc gaggactgca acgacaacgg cgacggcttc
     2821 gtcgccgtgc tgagcggcga cctcgggacg ctgctccagg ccagttatct cggcggcacc
     2881 gacatcgacg atgcgcgtgc gctcgcgctc gcgccggccg gtgccggcga tcccggcgcc
     2941 gtctacgtgg cgggccggac gatttccgac gattttcccg ccaccggcgg cggagcacag
     3001 ccgatcgcgc aacccggcag ctacggcggc ttcatcgccg ccctcagtcc ggatctcacg
     3061 gaaatcgtgc aggcaaccta tctgggcggg acgagcgtgg acgaagcgct cgccgtcgct
     3121 tacgattccg aaaacaatga aatctacgcg gccggagata ccagctccgc cgatttcccc
     3181 tgcaccttgg ccggcgcgcc gcctccggcg ggaggcagtt gcggctcgag cggctccggc
     3241 gcccagtcga gttatgccgg cgccttcgat gccttcgtcg cggcgctcaa tgcggatctc
     3301 accggcctga tccagtcgag ctatgtcggc ggcagcgccc tcgatcaggc cctgaccctc
     3361 gccaccgctt cgggcggcat ctacatcgcc ggtcagacct attcttcgga tcttcccgcg
     3421 atctccggag ccgcgcagcc gctctacgac agcaacggcg atggctttct cgccaaggtc
     3481 aatccggcac tcaccgcctt cgtgcgcacg acctatctcg gaggaagcgg cctcgacgcg
     3541 gccgatgccc tcgcgctcgg tcccgccggg caggtgtacg tcgccggcag cacccagtcc
     3601 accgactttc cctgcacggc ctcgagcggt cccgatccga acggcggagt ctgcgccgcg
     3661 gcgcacgccg gcgcccagcc caatgcgggc ggcggcggcg atgcctactc ggcgcttctg
     3721 agtgccgatc tcggccgcct cacgcaggcg agctatctcg gcggcggcgc tcccgatcag
     3781 ggcaacgcgc ttgcgctcgc gccggcgagc gggagcgtct cggggaatgt ctatctcgtc
     3841 gggcttacca actcggtcaa tttccccggc accgccggcg ccgcgcagcc ggcctacgcg
     3901 ggtagcagcg atgcctatgc cgccgtgctc tcgcccgatc tcaccggtcc gcaactggtc
     3961 atgaccctcg cggtgaccgc gcccgcgacc gcgcggcgca ataccttctt cacctacaac
     4021 gtcgacatca ccaatacgac cgcaccatcg gcggagttcg acggcaacgc gaccggcgtc
     4081 gtctttacgg acgtgctgcc gccgcaggtg acggtaaaga agatgacgtc cagccagggc
     4141 gatgactgca cccagctttc gggcgtggtg agctgcaatc tgggcaccat tgcggcgaac
     4201 gggggcgatg cgaccgtgac cgtcaaggtg ctcgcgacca attccggaag cgcggcgaat
     4261 gcggcgttca ttcgcgcgga tcaggcattg tcggaggatt cggcttcgag cgtgacgacc
     4321 acgacgagca ttcctttccc caattccggc aacggcggcg cgagcggctt cggctttgcg
     4381 ggactgctcg cactggcggt gttggggctc ttcggatacc tgcttcgccg tcgctgacaa
     4441 acggagcgag cggctcgcgc ttccgcttcc ggcgcccgga atccgttttg gtgctggttg
     4501 ccgtcgttgc aggcccggtg cttctcctcg agcgacgaac tccgcccggc ctctggcagt
     4561 cggtcacggg ctcgctgcgc tggggcgaaa ccgcggccgc ggcggccgcc cgcgagcttg
     4621 cggaagaaac cgggatcgaa gccgcggggc gcatcgaaga tctcggcagc ggcgcgcgct
     4681 ttcgcatcct tgccgcgtgg cgccaccgct tcgcccccgg ggtcgtcgac aatgacgaac
     4741 actggttccg gctgtggctc gatcgcccgg tcgccgtgcg gctcgcgccg cgcgagcacg
     4801 gccgcttcgc atgggtttcg ccgacgcagg cggcggcccg cgtgttctcc tggagcaatc
     4861 gcgccgcgat cgagcgttac cttgcgcgcg attgacgtcc gcgcggcgcc accgatcgaa
     4921 acagctcttc gagccgctcg aggtgcgttt cgagtccgaa ttgcgcgacc acatcgtcat
     4981 ggccggcctg tgccagtcgc cgcgcgaatg gcgcatcgcc cgcgaggcgt tcgagccgat
     5041 cggcaagcgc ggatacgtct cccggcgcgt agctgaggcc ggtctccccg tcgcgcacga
     5101 tctcggcggt gccgccggcc gccgtgcaca ccacgggcac gcgcatctgc agggcctcga
     5161 cgagcacgag accgaaggtt tcggccgccg acggcatgac caccacgtcg cagcaggcca
     5221 tggccgggcg cgcatcggcg aggacgccgg cgaagcgcag gcgctcggcg atgccgagcc
     5281 ggcgcgcgct cgcacgaagt tcttctgcgt agacctcgtc catcaccggg ccggcgatga
     5341 cgagcgaggc gtcgatgccg cgcccgacga gggtcgcgag tgcggcgatg agttcctgct
     5401 ggccctttgc gcgctcgatg cgcgagaagc agccgatgag aaagcccgtt cccggcgggc
     5461 gcagctcgcc acagtcgccc ggcggcggcg gatcggagat gccgagcggc agcacccgga
     5521 tgcgttcggg cggcagcggc agacgctcgc gcgcttcggc cgcgacgcgc gccgtgaccg
     5581 cgaggaagag atccacctcg cgatgcagcc agcgatgtac cggacttttt cgccggtcgc
     5641 ggctgatcgc catgtgtcgc gtatagacga gcgccgggtg tccggcggcc gcgcgtttga
     5701 cgagaaccgc gagcggcaga tcggcgctgc gatgaatgtg aaggacatcg gcctcgcgag
     5761 cgaggcgcgt gagcgcgggg aaatttcccc agggcgagag gcggcccgaa ttgatctcca
     5821 gcgccgcaac cgcttgcgcg agccgcgcga attcgcttcc tcgccgcgcg acggccttcg
     5881 catcgacgcc gcgcgccgca agctgcggcc cgaggccgcc ggcatatcgt tccagtccgc
     5941 cacgcccgac cgacaggcaa agctcgatca cgatcatgcg gtggggactt tcaggcggaa
     6001 gcggggcata atcgggcagg catgcaggcc gcgacaccga ccccgattat agagacgacg
     6061 acgccccgag tcgtcttcgt tcacggtctg ttcatgagcg gtcgcgaagg cgtcctgctt
     6121 tcccggcgtt tacgccggca ggcgcttcct tgcgagcgat ttgtttactc ctccttgcgc
     6181 gacaatcccg aggaggccgc ggcgcggctt gccagccgcc tgcgcgcgga gccggcgctc
     6241 catctcatcg ggcacagtct cggtgccgtc atcgtcgcga tggcaatcgc cgcggtgccc
     6301 gaatggcggg gccgggccgt gctgctcggg ccgccgctcg cgggcagcgt cacggcgcgg
     6361 cgcgcggcgg tgcttcccgg cggccgccgt ctgctcggca gcggcggaag gttccttctc
     6421 ggggagcttc acttcgggcg ctcttcccgc cgggtgctcg tcatcgccgg cgtgcgcaat
     6481 tttggcgtcg gccgtctgat cgggagttgc ccgggacccg gagacggtca ggtacggctg
     6541 gtcgaaacgc gtctggcggg cgccgcccat cgcagcgtcc gccgcggaca catggcactc
     6601 ctcttcgatc ggcgagtcgc ccgcgcggtg gcgagctttt ttcgcgacgg caatccgccg
     6661 ggcgcgactc tccccgctcc atcgcccggc taccgacgcc tctgagaagc ttccgacttt
     6721 ctaattcccg tccgcgagtt ttttgagttc gtagagaagc gcgagcgcgt cgcgggcact
     6781 gagcgcatcg ggatcgagcg ccgcgagcct tgcatgcagc gggtcgggtt ggggcgcgga
     6841 ggtctcgggc gcaaacaatt gtccctgtcc cgaggcgtgg ggacgattcg cctccagctc
     6901 ggcgag
//
LOCUS       DASUJG010000197         2869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_548323, whole genome shotgun sequence.
ACCESSION   DASUJG010000197 DASUJG010000000
VERSION     DASUJG010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548323"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1275)
                     /gene="ffh"
                     /locus_tag="VFX38_04115"
     CDS             complement(<1..1275)
                     /gene="ffh"
                     /locus_tag="VFX38_04115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX5314080.1"
                     /translation="MALFGQLSASLSAALGRLGGRGRLTEANIEETLVDVRLALLEAD
                     VALPVVEKFIEAVKVRALGAEIAKSLTPGQAFVRIVHDELTAMLSRGDPTLKLRVRPP
                     AVILLAGLQGSGKTTSAAKLAARLAGERKRVLLVSTDVQRPAAIEQLEKLAKSIGVGF
                     VPADGEQPLAIARRALARARAELTEVLVVDTAGRLHVDEALMTEIRALADLLAPIETL
                     FVADAMTGQDAVRSAAAFGETLPLTGIILTKTDGDARGGAALSAAEVTGRPIKFLGTG
                     EKTDALEAFDPARMAGRILGMGDLVGLAETVARKGDQKEADALAGKLRSGRKFDLADF
                     RGQIEQMLKVGGARALLEHLPGADKLADAAAAVDERALRRQMAIVDSMTPRERHHPAL
                     INGSRRRRIAAGSGNEVQDVNRLLRQFQQAEKM"
     gene            complement(1468..1842)
                     /gene="panD"
                     /locus_tag="VFX38_04120"
     CDS             complement(1468..1842)
                     /gene="panD"
                     /locus_tag="VFX38_04120"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015282303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEX5314081.1"
                     /translation="MQLTMLKAKLHLAVVTSADLDYEGSCAIDQELLARAGILEYEQI
                     HLYNVTNGERLVTYAIKAPPGSREIGANGAAAHHMRVGDRIIICSYVAMDEAVALRFR
                     PRVLILDEANRVKSERGGVRAA"
     gene            complement(1891..2733)
                     /gene="panC"
                     /locus_tag="VFX38_04125"
     CDS             complement(1891..2733)
                     /gene="panC"
                     /locus_tag="VFX38_04125"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX5314082.1"
                     /translation="MASVAGLRAWRAMRWRDGKTVALVATMGNLHAGHAALIRRAREI
                     ADFVLVSVFVNPTQFGPGEDYSGYPRSLAADHALSGESGADAVFAPSLAAVYPGGEGE
                     ITRVSVPGLTQILCGASRPGHFDGVTGVVTRLFHLAQPDFAIFGEKDYQQLVILRRMT
                     ADLLFGIEIVAEATVRASDGLALSSRNQYLEPAERARAPALYAALEKAAAALHSGARA
                     SDVEAGGLHALEAAGFRPEYFSVRRADDLGVPQADSQLRVLAAAWLGKARLIDNIAVP
                     PQRP"
     gene            complement(2744..>2869)
                     /gene="panB"
                     /locus_tag="VFX38_04130"
     CDS             complement(2744..>2869)
                     /gene="panB"
                     /locus_tag="VFX38_04130"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017374063.1"
                     /note="catalyzes the formation of tetrahydrofolate and
                     2-dehydropantoate from 5,10-methylenetetrahydrofolate and
                     3-methyl-2-oxobutanoate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX5314083.1"
                     /translation="GHKPRFAKNFMAGAGSVRAALAAYVAAVKSGEYPAPEHGFE"
BASE COUNT          497 a          970 c          949 g          453 t
ORIGIN      
        1 cattttctcg gcctgctgga actggcgcag cagccgattc acatcctgca cctcgttacc
       61 ggagccggcc gcaatgcgcc gccggcgcga gccgttgatg agcgcgggat ggtgacgctc
      121 gcgcggcgtc atcgaatcca cgatcgccat ctggcggcgc aaggcgcgct cgtccaccgc
      181 cgccgcggcg tcggcgagtt tgtcggcgcc gggcagatgc tcgagcagcg cgcgggcgcc
      241 gccgaccttg agcatctgct cgatctgccc gcgaaagtcc gccaagtcga attttcttcc
      301 cgagcgcaac tttccggcga gtgcatccgc ctccttctgg tcgcccttgc gggcgacggt
      361 ctcggcgagg ccgacaagat cgcccatccc gaggatccgc ccggccatgc gcgccggatc
      421 aaaggcctcc aaagcatcgg tcttctcgcc ggtgccgagg aacttgatcg gccgtccggt
      481 cacctctgcc gccgaaagcg ccgcgccgcc gcgcgcatcg ccatcggtct tggtaaggat
      541 gattccggtg agcggcagcg tctcgccgaa ggcggcggcc gagcgcaccg catcctggcc
      601 ggtcatggcg tcggcgacaa acagcgtctc gatcggcgcg agaagatcgg cgagcgcccg
      661 gatttccgtc atgagcgcct cgtccacgtg caagcgcccg gcggtgtcca cgaccagcac
      721 ctcggtgagt tcggcgcggg cacgcgcgag cgcgcgccgc gcaatcgcga gcggttgctc
      781 cccgtcggcg ggaacaaagc caacgccgat gctcttcgcg agtttctcga gttgctcgat
      841 tgcggcgggc cgctgcacgt cggtggagac gagaagcacg cgcttgcgct cgcccgcgag
      901 acgggccgcg agcttcgccg cgctcgtcgt ctttcccgag ccctgaagcc ccgcgaggag
      961 aatcacggcc ggcgggcgca cccgaagttt gagcgtggga tcgccgcgag agagcatcgc
     1021 cgtaagttcg tcgtgcacga tccgaacgaa ggcctgcccc ggggtgagac tcttcgcgat
     1081 ctcggcccca agagcgcgga cctttaccgc ttcgatgaat ttttcgacga cggggagcgc
     1141 gacatcggcc tcgaggagcg cgaggcgtac gtcgacgagc gtctcctcga tattcgcttc
     1201 ggtcagacgt ccgcgcccac ccaagcgccc aagcgcagcg gaaagacttg cggaaagctg
     1261 gccgaaaagc gccacgttat ggcctcaggg attcacaaaa agatggccgg acgctttcga
     1321 cgcacagacg tcggaaagcc caagggaatc gccgacagct tgcgcatcgt tcccgtgtcc
     1381 cggaagcgga tcggcctgtt tcgaggatta caagatggaa cagaaggtga atgcaccatc
     1441 ttcccggcgt tcgccgcgta ggcctcgtca ggcggcacgc acgccgccgc gttcgctctt
     1501 cacccggttg gcctcgtcga gaatcagcac ccgcgggcgg aagcgcagcg ccaccgcttc
     1561 gtccatcgcg acgtagctgc agatgatgat gcgatcgccg acccgcatgt gatgggcagc
     1621 ggcgccgttg gcgccgattt cgcgcgaccc gggcggggcc ttgatcgcgt aggtgacgag
     1681 ccgttcgccg ttggtgacgt tgtaaaggtg aatctgctcg tattcgagga tacccgcacg
     1741 cgccagaagt tcctgatcga tcgcgcagga accctcgtag tcgaggtccg ccgaggtgac
     1801 cacggccaga tgcaattttg ccttgagcat ggtgagctgc aatgggtcct ccgtcccgcg
     1861 agggacgccg ccaaaagggg aaaatcgtcg ctagggccgc tgcggcggca ccgctatatt
     1921 atcaatcagg cgggccttcc ccagccaggc cgcggcgaga acccgcagtt gcgagtccgc
     1981 ctgcggcact ccgagatcgt ccgcgcgccg caccgagaag tactccgggc ggaatccggc
     2041 ggcctcgagc gcgtgcaggc ccccggcctc cacgtcggac gcacgcgcac cggaatgcag
     2101 ggccgccgcg gccttttcga gcgcagcgta gagagccggc gcacgtgccc gttccgcggg
     2161 ctcgagatat tgattgcgcg aggaaagcgc gaggccgtcg ctcgcgcgta ccgtcgcctc
     2221 cgcgacgatc tcgataccga agagaagatc cgccgtcatc cgccgaagaa tcacgagttg
     2281 ctggtaatcc ttctcgccga agatcgcgaa gtccggttgc gcgagatgaa acagtcgcgt
     2341 cacgactccg gtaactccgt cgaagtgacc gggacgcgaa gcgccgcaaa ggatctgcgt
     2401 caatccggga accgacacgc gggtgatttc gccctcgcct ccaggataga cggcggcgag
     2461 ggacggtgcg aaaacggcat cggcaccgct ttcccccgag agcgcatgat cggcggcgag
     2521 actgcgcggg tagccggagt agtcttcgcc ggggccgaac tgcgtcggat tgacgaacac
     2581 cgacaccagc acgaaatcgg cgatctccct ggcgcgccgg atcaatgcgg catggcccgc
     2641 atgcagattc cccatggtcg caacgagcgc gaccgtcttg ccgtcgcgcc agcgcatcgc
     2701 gcgccaggcg cgcagccccg caaccgaggc gatgagcgcc gcgctactca aagccgtgtt
     2761 cgggcgcggg gtactcgccg ctcttgaccg cggcgacata ggctgcgagc gcggcccgca
     2821 cgcttccggc gcccgccatg aagtttttcg cgaagcgcgg cttgtggcc
//
LOCUS       DASUJG010000198         6887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_426367, whole genome shotgun sequence.
ACCESSION   DASUJG010000198 DASUJG010000000
VERSION     DASUJG010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6887
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426367"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VFX38_04135"
     CDS             <1..197
                     /locus_tag="VFX38_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014404528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEX5314084.1"
                     /translation="CLALAASYLPTLLYYRRNPLAALVLPVVATMYLAMTWFSALRAL
                     AGTRSSWKGRRYRTEARSEK"
     gene            336..1625
                     /locus_tag="VFX38_04140"
     CDS             336..1625
                     /locus_tag="VFX38_04140"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX5314085.1"
                     /translation="MTFKWLPDAALDPHPRLTWFTECCFDLTVLAFYRKEEILPPDYG
                     LAPGTLVVSNHQRDADVPILTTTLCQRRGLHIRWPLPFYAMREDLLRRGFLAGHLAHW
                     PRPLPALLGTLSLGWFFRIVRGEPLRRLREFTFGETLSALLAAGFADARCEAVFNARG
                     RREIEAAIGALPATLRNLDLRRQGKLRRGLWGLRRLRLETFRSLKPLFRREIAAQLAR
                     FSSLLDAGRVVYFAPEGTISANGRFGRVREGAWRIVRSAAAPPPILAIALSYDPLGPG
                     RLRVIVRVGIPMREYDTRERRNFEAALEREIRALYPLNASHLLARYLRRGADRFTTED
                     FAGWFAAARDALRSAGFALDPLLANREAQELAGERLRWLERKALVLRDGHCWRKSRDA
                     DVPPGWRKPAAIVAYFDNALADHLAALAPGVEGDIAP"
     gene            1622..2650
                     /locus_tag="VFX38_04145"
     CDS             1622..2650
                     /locus_tag="VFX38_04145"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX5314086.1"
                     /translation="MKGAHLLAVMLRPKIVLPIVLAAALFAVAFKLGDLGAVLARVRA
                     IPLWAMALALAGAIVYLAFKLLQFRHLVTNLGLRPDWRRLTLAFCVGELAVTLPFGIF
                     AQNWILAVGSKEDFGRSSAATVVMLLTEILVVLLFLAVIGIPGWPELRPLAIVFVLGL
                     LVVVFGVLRYGHVARRLGHRVQRRGLRRVLLQFIELLRGLRRLSRPQLLLLNVLIAGV
                     YLGALAFAFFVTGRAVGIANLSFLTAATIYAFSLAVLLLFGGILSQIGTMEVLGMSAS
                     QAWGLSLTQGLALMLGFRIVWTGAMWLVNLPLVFFLWRSIEHRHAKPVASPAAAIAPS
                     QPAAKAAD"
     gene            2814..3809
                     /locus_tag="VFX38_04150"
     CDS             2814..3809
                     /locus_tag="VFX38_04150"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX5314087.1"
                     /translation="MRVLVRPKVLLPILLAAALIFLALSLGDLSQVVGRIQAIPVTIM
                     AIALGFAALYLALKALQLHLLLDNLGAHPDWGRFALAFAVGELAVTLPLGIFAENWVL
                     AEEKKIKFGRSSAATVTMLLAEIAIVLLLLTVVGIPGWRILQPLAALVLFLIAAFLFG
                     VLHFEPQANRLAHKIKQPKLHEAALAGIGLIRGLKSLTNWHVLAVNLVIPVFYLGALA
                     CAFLAVGHGVGLHSLDYLKAATIYAFSLAAVLLCGGLVGQIGSVEIVGMIAAQAWGIG
                     YTDGLALMLGFRVVWTGALWLLNGPIVIAFWRSKRPHADDETDDASADHREKTSD"
     gene            complement(3777..4856)
                     /locus_tag="VFX38_04155"
     CDS             complement(3777..4856)
                     /locus_tag="VFX38_04155"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5314088.1"
                     /translation="MAMRILFAVHDWGLGHAARSLVLIRALLARGDEVTVLMADSAGL
                     QLLKNELGASCAFRVYRDIPKPFSRWPFMFYLRMSLSMPWVWLHYKLEHRLTEKLVAE
                     RGFDLVVSDSRFGIWSRAVPSYCIFHSLRQIIPGRLRWMERLVEWGQRRLLKHYRKVL
                     IPDVEEGGGLSGDLGHYPALDWGEGRLVYLGPLADVARPGLPEDIDYFFSVSGIEPQR
                     GILERRVLAALPELRGCIVVTLGRPELAGEERQIAGATVYGYLDRARQAEMLNRAHVI
                     VTRSGYTTLMELATLGKRALFVPTPGQSEQEYLAHFHHERGQVWATTQGRLDIPRDLV
                     HAAAASGLPRLSAAESVGRFLAVIG"
     gene            4988..>6887
                     /locus_tag="VFX38_04160"
     CDS             4988..>6887
                     /locus_tag="VFX38_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEX5314089.1"
                     /translation="MNTPLPTAEVSLKELTVRGLILGILITLVFTAANVYFGLKAGLT
                     FSTSIPAAVISMAILRWLRNPTMQENNIVQTVASAAGTLSAIIFVLPGLVIIGWWQGF
                     PFWMSFGICATGGILGVMYTIPLRRALVTDSDLPYPEGVACAEVLKVGSGEEKDPDAG
                     AVEAKGAGLKAVTIGGIVAAIFYVIVQTKVFAASLSGYFHFGGGKSGATGFDFSLSFA
                     LLAIGHLVGLWVGISMLVGAIVGWGWLVPYYSIMHPGVADAMTVAQGAWSHSVRFIGA
                     GTIGVAAIWTLVKLVKPVIAGLAGALAAARVRKSGQGHTLPQTERDMPIGIVGIITLV
                     CLIPIAYLLWHFSGISGLGAAMTTLVIGGVVFVVILSFLVSAVCGYMAGLIGSSNSPL
                     SGVGILVVVIAALLLVFGVKDQLPAGTGKALVAFALFLTSVVFAVASIANNNLQDLKT
                     GQLVRATPWKQQAALVIGVIAGAFVIPPVLDLLSKAYGFLGAPGVDHPSHALPSPQAG
                     LISALAQGVIQSSIDWSLIGIGCAIGVGAIILDEILARTAKHARLPPLGVGLGIYLPT
                     STTLMVVVGAIVGWWFNRRAERKAKPEALKQLGVLLASGLIVGESILGVIFAAAVAFS
                     GSASPIALV"
BASE COUNT         1022 a         2381 c         2152 g         1332 t
ORIGIN      
        1 cctgccttgc gctcgccgcg agttatcttc cgacgctcct ttattatcgg cgcaacccgc
       61 tcgccgccct agtgctgccg gtcgttgcca caatgtatct ggccatgacc tggttctcgg
      121 cgctgcgcgc gcttgccggc acccgctctt cgtggaaggg ccgccgttac cggacagaag
      181 cgaggagcga gaagtgagaa gcgagagacg aaaagatttg gcaggccggg ataagcgcgc
      241 atttgcgcgc gttcccggca ttaaagcgga acgccgggaa cgctccgctt atcccggcct
      301 gcacagttct catctcgcct tgcggagcga tcgccgtgac gtttaaatgg ctgccggacg
      361 ctgcactcga tccgcatccg cgactcactt ggttcaccga gtgctgcttc gatctcacgg
      421 tgcttgcgtt ctatcgcaag gaagagatct tgccccccga ttacggcttg gcgccgggca
      481 cgctcgtcgt ctcgaatcat cagcgcgacg ccgacgtgcc gattctcacg accacgctct
      541 gtcaacgccg gggcttgcac attcgctggc cgctgccgtt ctacgcgatg cgcgaggacc
      601 ttctgcgccg cggctttctc gccggccatc tcgcgcattg gccgcgcccg ctgccggcgc
      661 tactcggaac cctttcgctc ggctggttct ttcgcatcgt gcgcggagag ccgctgcgcc
      721 gcctgcgcga attcaccttc ggcgaaaccc tgtccgcgct tctcgcggcc ggcttcgccg
      781 atgcgcgatg cgaggcggtc ttcaacgccc gtggccggcg cgaaatcgaa gcggcaatcg
      841 gcgcgctgcc ggcgacgctt cgcaatctcg atctgcgacg gcaagggaaa ctccgccgcg
      901 gcctctgggg cctgcggcgc ctgcgcctcg agacgtttcg cagcctgaag ccgctcttcc
      961 gccgcgagat agcggcgcag ctcgcgcgat tctcctcgct cctcgacgcg gggcgggtcg
     1021 tgtacttcgc gcccgaagga acgatttccg caaacgggcg tttcgggcgc gttcgcgaag
     1081 gcgcctggcg tatcgtccga tccgcggccg cgcctccgcc gattctggcg atcgcgcttt
     1141 cctacgatcc gctcggcccg ggacgcctgc gcgtgatcgt gcgggtgggc attccgatgc
     1201 gcgagtacga tacgcgggaa cgccgaaact tcgaggcggc tctggagagg gaaatccgcg
     1261 cgctctatcc gctcaacgcg agtcatctcc tcgcccggta tctgcggcgc ggcgcggatc
     1321 ggttcacgac ggaggacttc gcaggctggt tcgccgcagc ccgcgacgcc ctgcgctccg
     1381 ccggtttcgc gctcgatccg cttctcgcta accgcgaggc gcaggaactc gccggcgaac
     1441 gattgcgctg gctcgaacga aaggcgctcg tcctgcgcga cggccattgc tggcgaaaat
     1501 cccgggacgc ggatgtgcct cccggttggc gcaaacccgc ggcgatcgtt gcctacttcg
     1561 acaatgccct cgccgaccat ctcgccgcgc tcgcgcccgg cgtcgaaggg gacatcgcgc
     1621 catgaaggga gcgcatctgc tcgccgtgat gctgcgcccg aagatcgtgc tcccgattgt
     1681 tctcgcggcc gcgctgttcg cggtcgcctt caagctcggc gatctcggcg cggtgctcgc
     1741 gcgcgtccgg gcgattccgc tgtgggcgat ggcgctcgcg ctcgcgggcg cgatcgtcta
     1801 tctcgccttc aagctcttgc agttccgcca tctcgtcacc aatctcggcc tgcgtccgga
     1861 ttggcgccgc ctgacgctcg ccttctgcgt cggcgagttg gcggtgactc tgcccttcgg
     1921 aatcttcgca cagaactgga ttctcgccgt cggcagcaag gaggatttcg ggcgcagttc
     1981 cgccgcgacg gtggtgatgc tgctcaccga aatcctggtc gtgctgctgt ttctcgcggt
     2041 gatcggcatt cccggctggc ccgaactgcg cccgcttgcg atcgtcttcg tgctcgggct
     2101 tctcgtggtc gtcttcggcg tgctccgcta cggacatgtc gcgcgccgcc tcggccatcg
     2161 cgtgcaacgg cgcggcttgc gccgtgtgct actccaattc atcgaactgc tgcgcggcct
     2221 gcgtcgcctg tcgcgcccgc agcttctgct gctcaacgtc ctcattgccg gcgtgtatct
     2281 cggcgcgctc gccttcgctt tcttcgtgac cgggcgcgcg gtcggaatcg cgaacctgag
     2341 cttcctcacg gccgcgacga tctacgcctt ttcgctcgcc gtgctgctcc tcttcggcgg
     2401 gatcctgagc cagatcggca cgatggaagt gctcggcatg agcgcctcgc aggcctgggg
     2461 cctgagcctg acccaggggc tcgcgctgat gctcggcttc cggatcgtct ggaccggcgc
     2521 catgtggctg gtaaatttgc ctctggtttt ctttctgtgg cgctcgatcg aacaccgcca
     2581 cgccaagccg gttgcttcgc ccgcggcggc aatcgcgcca agtcaaccgg cggccaaagc
     2641 cgccgactga acccgcggcc ccggcgttcg cgcgcgtccc aaccgtcggc gtggcccgcg
     2701 gcgccggcgg cgtgcgagta agatattttt tatcggcgcc gggacaagaa tcataccttc
     2761 gtgaacgcga gccaccccgc ctccccgtcg cgaccatgaa cttgcccggc gccttgcgag
     2821 ttctcgtccg gcccaaggtt cttctgccga tattgctcgc ggccgcactc atctttctgg
     2881 ccctcagcct gggcgatctg agccaggtcg tcgggcgcat ccaggcgata ccggtcacca
     2941 tcatggcgat cgcgctcggt ttcgccgcgc tctatctcgc gctcaaggcg ctgcaactgc
     3001 atctgctgct cgacaatctc ggcgcccatc ccgattgggg tcgctttgcc ctggcctttg
     3061 cggtcggtga acttgcggtg acgttgccgc tcggaatttt tgccgaaaac tgggtgctgg
     3121 ccgaagagaa gaagatcaaa ttcggtcgca gttccgcggc caccgtcacg atgctgctgg
     3181 cggaaatcgc gatcgtgctt ctcttgctca ccgtggttgg aattcccggg tggcggatat
     3241 tgcaaccgct tgcggcgctg gtgctgtttc tcatcgctgc gttcctgttt ggcgtgctgc
     3301 acttcgagcc ccaggccaat cgcctggcgc acaaaatcaa gcagccgaag ctgcatgaag
     3361 ccgccctggc gggaatcgga ctgattcgcg ggctgaaaag tctgaccaac tggcacgtgc
     3421 tcgcggtgaa tctggtgatt ccggtgtttt atctcggcgc attggcctgc gcctttctcg
     3481 cggtcggcca cggcgtcggc ctgcattcgc tcgactatct caaggctgca acgatttacg
     3541 ccttttcgct cgcggcggtg cttctgtgcg gcgggctggt cgggcagatc ggcagcgtgg
     3601 aaatcgtcgg catgatcgcc gcccaggcct ggggcatcgg ttacaccgac gggctcgcgc
     3661 tgatgctcgg ctttcgcgtg gtctggaccg gcgcgctgtg gctcctcaac ggcccgatcg
     3721 tgattgcgtt ctggcgctcc aaacgaccgc acgccgacga cgaaaccgac gatgcctcag
     3781 ccgatcaccg cgagaaaacg tccgactgat tcggccgcgg aaagacgcgg cagcccgctg
     3841 gccgccgcgg catgcacgag atcacgcgga atgtcgagac ggccttgggt cgtcgcccac
     3901 acctgaccgc gctcatggtg aaagtgcgca agatattcct gctcgctttg acccggcgtc
     3961 ggcacgaaga gcgcgcgctt gccgagcgtc gcgagttcca tcagcgtcgt atagccggag
     4021 cgcgtgacga tgacatgcgc acggttgagc atctcggcct ggcgcgcgcg atcgagatag
     4081 ccgtaaaccg ttgcacccgc aatctgcctt tcctcgcccg cgagttccgg gcggccgagc
     4141 gtcacgacga tgcagccgcg cagttccggc agcgcggcaa gcacccgcct ttccagaatc
     4201 ccgcgttgcg gttcgatgcc ggagacggaa aagaaataat caatgtcttc cggcaaaccc
     4261 ggacgcgcga catcggcgag cgggccgagg taaacgagcc gtccctcgcc ccagtcgagc
     4321 gccggataat gaccgaggtc gcccgaaagc ccgccgccct cctccacgtc cgggatcagc
     4381 accttgcggt aatgtttgag caggcgacgc tgcccccact ccacgaggcg ctccatccag
     4441 cgcagccgtc ccggaatgat ctggcgcagc gaatggaaga tgcagtagct cggcaccgcc
     4501 cgcgaccaga taccgaagcg gctgtcggaa acgacgagat cgaagccccg ttcggcgacg
     4561 agtttctcag tcaggcgatg ctcgagcttg tagtgcagcc acacccaggg catggacagg
     4621 ctcatgcgca ggtaaaacat gaacggccag cggctgaagg gtttcgggat atcgcggtaa
     4681 acgcggaacg cgcagctcgc gcctagttcg ttcttcagaa gctgcagccc cgcgctgtcg
     4741 gccatcagca ccgtcacctc gtcgccgcgc gcgaggagcg cgcggatcag caccaggctg
     4801 cgcgccgcgt gcccgagccc ccagtcgtgg accgcaaaga gaatccgcat cgccatcgac
     4861 cctgccgccc caattgcgcg cgtatcgtac caacaagctg ctacacttcg gcgacccgcg
     4921 ggcgtctcgc cggccgatcg gagagcccgc gtccgaccgc agcagtccga tccaaggggg
     4981 ttcgaacgtg aatacaccgc tgccgaccgc ggaggtttcg ctcaaggaac tgaccgttcg
     5041 cgggctgatc ctcggcattc tcatcacgct cgtgttcacc gccgcgaacg tctatttcgg
     5101 tctcaaggcg gggctcacct tctcgacctc gattcccgcc gccgtgatct cgatggcgat
     5161 cctgcgctgg ctgcgaaacc cgacgatgca ggagaacaac atcgtgcaga cggtggcctc
     5221 ggccgcgggc acgctctccg caatcatctt cgtgctgccg gggctcgtca tcatcggctg
     5281 gtggcaggga tttccgttct ggatgtcctt cggcatctgc gcgaccggcg gcattctcgg
     5341 cgtgatgtac acgattccgc tgcgccgcgc gctcgtgacc gattccgatc tcccctatcc
     5401 ggagggcgtc gcctgcgccg aggtgctcaa ggtcggctcc ggcgaagaga aagacccgga
     5461 cgcgggagcc gtcgaagcca agggcgcggg actcaaggcc gtgaccatcg gcggcatcgt
     5521 cgccgcgatt ttctacgtga tcgtgcaaac caaggtcttc gccgcgagcc tctccggcta
     5581 tttccatttc ggcggcggca aaagcggcgc gaccggcttc gacttcagtc tctccttcgc
     5641 gctcctcgcg atcggccatc tcgtgggcct gtgggtcggc atctcgatgc tcgtcggcgc
     5701 gatcgtcggc tggggctggc tcgtgcccta ctacagcatc atgcatccgg gcgtcgcgga
     5761 tgccatgacc gttgcccagg gagcctggag tcacagcgta cgcttcatcg gcgccggcac
     5821 catcggcgtc gccgcgatct ggacgctcgt caaactcgtc aagccggtga ttgcgggact
     5881 cgcgggcgca ctcgccgccg cgcgggtgcg caagtcgggc cagggccaca cgctgccgca
     5941 gaccgagcgc gacatgccga tcgggatcgt cggcatcatc acgctcgtgt gcctgatccc
     6001 gatcgcgtat ctcctgtggc acttcagcgg catcagcggg ctcggcgcgg ccatgaccac
     6061 gctggtgatc ggcggcgtcg tcttcgtcgt catcctgagc tttctcgtct ccgccgtctg
     6121 cggctacatg gcgggattga tcggctcctc gaacagtccg ctctcgggcg tcggcattct
     6181 ggtcgtcgtg atcgccgcgc tgctgctcgt gttcggcgtc aaggatcagc ttccggccgg
     6241 caccggcaag gccctcgtcg cctttgcgct gtttctcacc tcggtcgtgt tcgcggtcgc
     6301 ctccatcgcc aacaacaacc tgcaggatct gaagaccggc caactcgtcc gcgcgacgcc
     6361 ttggaaacag caggccgcgc tcgtcatcgg cgtgatcgcc ggggccttcg tgattccgcc
     6421 cgtgctcgac ttgctgagca aggcctacgg cttcctcggt gcgccgggcg tggatcatcc
     6481 ctcgcacgcc ctgccgtctc cgcaggcagg actcatctcc gcgctcgccc agggcgtgat
     6541 ccagtcgagc attgattgga gcctgatcgg catcggctgc gcgatcggcg tcggcgccat
     6601 catcctcgac gaaatcctcg cgcgcaccgc aaagcacgcg cgcctgccgc cgctcggcgt
     6661 cggactcgga atttatcttc cgacctcgac gacgctcatg gtcgtcgtcg gcgccatcgt
     6721 cggctggtgg ttcaaccgcc gcgccgaacg caaggccaag ccggaggcgc tcaaacaact
     6781 cggcgtgctg cttgcctcgg gactcatcgt cggcgaaagc atcctcggcg tcatcttcgc
     6841 cgccgccgtc gccttctccg gttccgcctc tccgatcgcc ctcgtcg
//
LOCUS       DASUJG010000199         2909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1771556, whole genome shotgun sequence.
ACCESSION   DASUJG010000199 DASUJG010000000
VERSION     DASUJG010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1771556"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VFX38_04165"
     CDS             complement(<1..213)
                     /locus_tag="VFX38_04165"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269153.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX5314090.1"
                     /translation="MLIFEHSRAGRRARAQSPAGDFALDDLPAESLRSTPPALPEVSE
                     LDAVRHYTRLSQRNFSIDTHFYPLGSC"
     gene            complement(207..713)
                     /gene="tpx"
                     /locus_tag="VFX38_04170"
     CDS             complement(207..713)
                     /gene="tpx"
                     /locus_tag="VFX38_04170"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEX5314091.1"
                     /translation="MATIKLKGNPIHTSGELPRTGAAAPDFKLTRGDLSDVSLGAFAG
                     KKKLFNIVPSLDTPVCALSTRKFNEYAKAHPDAVMLVVSADLPFAQARFCGNEHLQNV
                     VPLAMFRDQKFATDFGVLLTDGPMAGLTARAVVVTDAGDKVRYTELVPEIAQEPDYEA
                     ALAALDAC"
     gene            complement(767..2140)
                     /gene="gcvPA"
                     /locus_tag="VFX38_04175"
     CDS             complement(767..2140)
                     /gene="gcvPA"
                     /locus_tag="VFX38_04175"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEX5314092.1"
                     /translation="MPFIPHTEEDVREMLAAVGVGGIGELFDEIPRELRIETLQGVPA
                     GLGEMEVSRLMRERAAGDGAALSFLGAGAYEHHIPAAVWELATRGEFYSSYTPYQAEA
                     SQGTLQLIYEFQSMMSALTGLAVSNASLYDGASALAEAALMALRIKRKDTTRRILVPA
                     ALNPHYKSVLATICGPQEISFHEIAWDPASGRLSERALADAPAAAALVVAQPNFLGVI
                     EDSERLARHARERELVLIGAVNPTPLALLTPPGAWGDAGADIACGEGQPLGVPMSSGG
                     PYFGFMTCRKEHVRQLPGRIVGRTTDAAGRGGFALTLQAREQHIRRSKATSNICTNQG
                     LAVTAATIYMALLGPAGLRAVAAASHARCRELLARCEAAGGARRFTAPFFHEAAIAVG
                     RDAGELLARMLAADDIIGGYDLAGLSPELSDSLLVCATETKTSADLERYGASLGAALA
                     QPARAQA"
     gene            complement(2148..2537)
                     /gene="gcvH"
                     /locus_tag="VFX38_04180"
     CDS             complement(2148..2537)
                     /gene="gcvH"
                     /locus_tag="VFX38_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX5314093.1"
                     /translation="MSEIPADRHYTQTHEWLRELSDGTLELGITEHAQHALGDLVFVE
                     LPEAGAELSTGEGCVVVESTKAASDVYSPVDGEVAAVNSALADRPELVNQDPYGEGWL
                     LRIRASGDLPAGLMDAAAYAACIESAG"
     gene            complement(2564..>2909)
                     /locus_tag="VFX38_04185"
     CDS             complement(2564..>2909)
                     /locus_tag="VFX38_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEX5314094.1"
                     /translation="NLAWTVAMDPAGRDFIGRAALERLRAAGEKRRLTGLVLRERGIL
                     RHGQSVSTEAGEGIVTSGGFAPTLGRSIALARLPAEAAAEAGVVIRKLKVAAAIVNPP
                     FVRRGKVLVRIA"
BASE COUNT          459 a          929 c         1053 g          468 t
ORIGIN      
        1 gcaggagccg aggggataga agtgcgtgtc gatcgagaag ttccgctggg aaaggcgcgt
       61 gtaatgccgc accgcgtcga gttcggaaac ttcgggaagc gcgggcggcg tactgcgcag
      121 agattccgcc ggcagatcgt cgagcgcgaa gtcgcccgcg ggcgactgcg cccgcgcccg
      181 ccgcccggcg cgcgaatgct cgaatatcag catgcgtcga gcgccgcgag cgccgcttcg
      241 taatcgggtt cctgcgcgat ttcgggcacg agttcggtgt aacgcacctt gtcgcccgca
      301 tcggtgacga ccacggcgcg ggccgtgagg ccggccatcg gtccatcggt aaggagcacg
      361 ccgaagtcgg tcgcaaactt ctgatcgcgg aacatcgcga gcggtaccac gttttgcaga
      421 tgttcgttgc cgcagaagcg cgcctgcgcg aagggaagat cggccgaaac gacgagcatc
      481 acggcatcgg gatgggcctt cgcgtattcg ttgaacttgc gcgtggagag cgcgcatacc
      541 ggcgtgtcga ggctgggcac gatgttgaag agtttctttt tgcccgcaaa ggcaccgagg
      601 gagacatcgg aaaggtctcc cctggtgagt ttgaaatcgg gggcggcggc gccggttcgc
      661 ggcagctcgc cgctggtgtg gatgggattg cctttgagtt tgatggtagc catgttgtat
      721 ctcctggttc gtcgaaggaa aattgcgagg ccgcgggcgc gtcttctcag gcctgcgcgc
      781 gggcgggctg cgcgagtgcc gcgccgagtg aagctccgta gcgttcgaga tcggcggagg
      841 tcttcgtttc ggtcgcgcag acgagcagcg aatcggaaag ctcgggactc agcccggcga
      901 gatcgtagcc gccgatgatg tcgtcggccg cgagcatgcg cgcgagcagc tcgccggcgt
      961 cgcgcccgac agcgatcgcc gcctcgtgaa agaagggagc cgtgaagcgc cgggcgccgc
     1021 cggccgcctc gcatcgcgcg agcagttcgc ggcagcgcgc gtggctcgcc gccgcaaccg
     1081 cacgcaggcc cgccggcccg aggagcgcca tgtaaatcgt tgcggcggtg acggcgaggc
     1141 cctggttggt gcagatgttg gaggtggcct tggagcggcg gatgtgctgt tcgcgcgcct
     1201 gcaaagtgag ggcgaagccg ccgcgtccgg cggcgtcggt cgtacgcccg acgatccgcc
     1261 ccggaagctg gcgcacatgt tccttgcggc aggtcatgaa gccgaagtag gggccgcccg
     1321 aactcatcgg cacgcccaga ggctggcctt cgccgcaggc gatgtccgcg cccgcgtcgc
     1381 cccaggcgcc cggcggcgtg agcagcgcga gcggggtcgg attcacggcg ccgatcagca
     1441 cgagttcgcg ctcgcgggca tggcgtgcga gccgctcgct gtcctcgatc acaccgagga
     1501 aattgggctg cgcgacgacg agcgcggccg ccgccggcgc atcggcgagg gcgcgctcgc
     1561 tcagccgtcc gctcgccggg tcccaggcga tttcatgaaa gctgatttcc tgcggcccgc
     1621 agatcgtcgc gagcacggac ttgtagtgcg ggttcaacgc ggcgggaacg agaatccggc
     1681 gggtcgtgtc cttgcgcttg atccgcagcg ccatgagcgc cgcctcggcg agcgccgagg
     1741 cgccgtcgta aagcgaggca ttggaaaccg cgagacccgt gagcgcgctc atcatcgatt
     1801 gaaattcgta gatgagctga agcgtgccct ggctcgcctc ggcctgatag ggcgtatagg
     1861 aactgtagaa ttcgccgcgg gtcgcgagtt cccacacggc ggcggggatg tgatgttcgt
     1921 aggcgcccgc accgagaaac gaaagggccg cgccgtcgcc cgcggcgcgt tcgcgcatca
     1981 ggcgcgagac ttccatctcg ccgaggcctg cgggaacgcc ctgcagggtt tcgatgcgaa
     2041 gttcccgcgg gatttcgtcg aacaactcgc cgatgccgcc gacgccgacg gccgcgagca
     2101 tctcgcgaac gtcttcctcg gtgtgcggaa tgaagggcac ggcgctttca gcccgccgat
     2161 tcgatgcagg cggcgtaggc cgcggcgtcc atcagccccg cgggaagatc gcccgaggcg
     2221 cggatgcgca gcagccagcc ctcgccgtag ggatcctggt tcacgagttc gggccggtcg
     2281 gcgagcgcgg aattgaccgc cgcaacctcg ccgtccaccg gactgtacac gtcggaggcg
     2341 gccttggtgg attcgaccac gacgcaacct tcgccggtgc tcagttccgc cccggcctcg
     2401 ggcaattcga caaacacgag atcgccgagc gcatgctggg catgttcggt gattccgagt
     2461 tcgagcgtgc cgtcggagag ttctcgcagc cactcgtggg tctgggtgta gtgccgatcg
     2521 gcgggaattt cactcatggt tttcctcctt gcggatgcgg ggttcaggcg atgcgcacga
     2581 gcaccttgcc gcggcgcaca aagggcggat tgacgattgc ggccgccacc ttgagcttgc
     2641 ggatgacgac gccggcctcg gccgccgcct cggccgggag gcgggcgagc gcgatcgagc
     2701 gccccagcgt gggggcaaag ccgccgctgg tgacgatccc ttcgccggcc tcggtcgaga
     2761 cgctctggcc gtggcggaga atgccgcgct cgcggagcac cagtcccgtg agccggcgct
     2821 tttcgcccgc cgcgcgcagg cgctccagcg ccgcgcggcc gatgaaatcg cggcccgcgg
     2881 gatccatcgc caccgtccag gctaggttc
//
LOCUS       DASUJG010000200         4294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_916846, whole genome shotgun sequence.
ACCESSION   DASUJG010000200 DASUJG010000000
VERSION     DASUJG010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916846"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VFX38_04190"
     CDS             <1..412
                     /locus_tag="VFX38_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX5314095.1"
                     /translation="ALPADSPLARRERFAISDLAGERLLLLEDGHCLRDQALEVCHLA
                     GANERAGFRASSLETLRHMVAAGVGITLLPVLATQPPVASAPGLRLRRFEAPMPHRRI
                     ALLWRESSPLGDLLHELAAAFRDLPADLLSPQGS"
     gene            complement(563..946)
                     /locus_tag="VFX38_04195"
     CDS             complement(563..946)
                     /locus_tag="VFX38_04195"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX5314096.1"
                     /translation="MHRIVIPVLMAAVATFAIAACHKQPANPNPAGQNPNAQIQQGAN
                     QVSQGVKAAVSDSAITAQVKAKLAANAGLDSADIHVETNNGVVTLTGTVDRIGLKDMA
                     ARTAHSVNGVKRIVNQIEVKPPSGG"
     gene            complement(1153..1329)
                     /locus_tag="VFX38_04200"
     CDS             complement(1153..1329)
                     /locus_tag="VFX38_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005770008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 family protein"
                     /protein_id="HEX5314097.1"
                     /translation="MLWWAVVFFIIAIIAAIFGFGGIAAGAITIAKILFIIFVILFII
                     SLIAWMVRGRRTRV"
     gene            1436..2377
                     /locus_tag="VFX38_04205"
     CDS             1436..2377
                     /locus_tag="VFX38_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEX5314098.1"
                     /translation="MEYKDYYKVMGVERKASPDEIKRAYRKLARRYHPDVSKEAGAEA
                     KFKEVAEAYEVLKDPDKRRAYDELGAEWKSGQDFRPPPQWEGGNFAFHTQGFPGADAG
                     DFSDFFESLFGGAQRRAGRTPLRARGRDEHARVDITLEDAFHGATRAISMRVPEADAA
                     GRVALRERTLNLRIPKAVKPGQQIRLAGQGGPGIGGGEAGDLYLEVQFRPHPRYRVEG
                     RDLVLMLPVAPWEAALGASVRVPTPAGPVELKLPPGSNAGRRLRLKGRGLPGKTPGDL
                     YVTIEIALPPADTEERREFYRRMARELPFEPRKGLEA"
     gene            2380..2694
                     /locus_tag="VFX38_04210"
     CDS             2380..2694
                     /locus_tag="VFX38_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020413782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HEX5314099.1"
                     /translation="MVQKSVAIVTGIVIDEERALSLEELARACSMQSEWVLELVAEGI
                     IEAASREPDEWRFPGRSLARARVAARLQRDLDVNLAGIALALDLLQEIEDLRARLGLA
                     GE"
     gene            2738..3463
                     /locus_tag="VFX38_04215"
     CDS             2738..3463
                     /locus_tag="VFX38_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX5314100.1"
                     /translation="MNPTLRLFAFSLLPVAVLIGAAAAGSLRAPGPRLRSAVLHFAGG
                     VIFAVVAVELLPDVVRARALLPTAIGFAIGTALMLALRAASRRFERRADAATAVLPLG
                     LLAAVGVDLLIDGLMIGIGFAAGARQGVLLAIALAFEMLALGLALAATLAKRGAARGK
                     ILVCIAALALLLIVGAVAGGAPLAGASGRALAGVLAFGSAALLFLVTEELLGEAHETQ
                     DSAALTAAFFLGFLVQLIIDMAH"
     gene            complement(3655..4242)
                     /locus_tag="VFX38_04220"
     CDS             complement(3655..4242)
                     /locus_tag="VFX38_04220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5314101.1"
                     /translation="MREPFAFLFCGRLLADKGLRELVQAAHILRTGGKNFVVRLAGPV
                     DDDNSSAISRRELDGWTAEGSVEYLGMLDDVRMALAAAHCVVLPSYYREGVPRSLLEA
                     AAMARPVITTDAPGCRDAVVAEKSGFLCKPRDVHSLARCMERMLALPPEALAAMGRAG
                     REYMEQNFSEEIVLDAYVNALADIAPRSTAPGARL"
BASE COUNT          684 a         1432 c         1409 g          769 t
ORIGIN      
        1 tgcgctcccg gcggacagtc ccctcgcgcg gcgcgagcgc ttcgccatct ccgatctcgc
       61 cggcgaacgc ctgctcctgc tcgaggacgg acattgtctg cgcgaccagg cgctggaagt
      121 ctgtcatctc gccggagcga acgaacgggc gggatttcgc gcaagcagtc tcgaaacgct
      181 gcggcacatg gtggcagccg gagtgggaat cacgcttctt ccggtactcg cgacccagcc
      241 tccggttgcg tcggcgccgg gcttgcgatt gcgtcgcttc gaggctccga tgcctcaccg
      301 ccgcatcgca ttgctgtggc gcgaaagctc gccgctcggc gacttgcttc acgaactcgc
      361 ggcggccttc cgcgatcttc ccgcggatct tttgagcccg cagggaagct agccgcgaag
      421 gcagccggct ccgtccgcgc gccccgcgat tgcagcaagc gcgaaagtcg gcgggtcgcg
      481 tctgcctgcc tcgcgacgag ggacgggatc gaaccgcgat tccgggtgcg gcccgatccc
      541 ttgcgtccgc ttcgatgccg cctcagcctc cgctgggcgg cttgacctcg atctggttca
      601 cgatccgttt gacgccgttc acgctgtgcg ccgtgcgggc cgccatgtcc ttgaggccga
      661 tgcgatccac ggttcccgtg agtgtcacga caccgttgtt ggtttcgacg tgaatgtcgg
      721 cgctgtcgag ccccgcgttc gccgcgagtt tggccttgac ctgtgcggtg atggcgctgt
      781 cgctcacggc cgccttcacc ccctgggaca cctggtttgc gccctgctga atctgggcgt
      841 tggggttctg gccggccgga ttcgggttcg ccggctgctt gtgacaagcc gcaatcgcga
      901 aggtcgcgac ggcggccatg aggaccggga tgacgattcg atgcatgggg gattctcctt
      961 gctgttgtgt cggtttagag gaaggcaatg ccgggtaagt tccggcgccg cccgcgcttt
     1021 cgcccggcag ggtgtcggaa aacactctgc cgatgcggga caggagcgtc ccgccgcgaa
     1081 tcaagccccg ctgcttgttc gcgacgactt gcggactcgg aaaaatacgg cgatgtactt
     1141 cccggaggct cgctatacgc gcgtccggcg accgcgaacc atccaggcga tcagcgaaat
     1201 gatgaacagg atgacgaaaa ttatgaaaag gatcttcgcg atcgttatcg cgccggcggc
     1261 aatgccgccg aacccgaaga tcgccgcgat gatggcgatg atgaagaaaa ctacggccca
     1321 ccagagcatg tgcatctcct gtatacgctt gatccgactc tagccgatcg ggggcctcct
     1381 ttgcacgtcc ccggtctgct atgctgggat gtcgccgacg tcgccccgtc ctcgcatgga
     1441 atacaaggac tactacaagg tcatgggcgt cgaacgcaag gcctcgccgg acgaaatcaa
     1501 acgcgcctat cgcaagcttg cccgccggta tcatcccgat gtcagcaagg aagcgggggc
     1561 cgaggcgaag ttcaaggaag tcgcagaagc ctacgaggtg ctcaaggatc ccgacaagcg
     1621 ccgggcctac gacgaactcg gggccgaatg gaagtcgggg caggacttcc gcccgccccc
     1681 gcagtgggaa ggcgggaact tcgcgtttca tacgcagggg tttccgggcg cggatgcagg
     1741 tgatttcagc gactttttcg agtctctctt tggcggtgcg cagcgccggg ccgggcgcac
     1801 accgctgcgc gcgcgcggac gcgacgaaca cgcgcgcgta gacattacgc tggaagacgc
     1861 ttttcacggc gcgacgcgcg cgatttccat gcgagtgccc gaagccgacg ccgccggacg
     1921 cgtcgcgctg cgcgagcgca cgctcaacct gcgcatcccg aaagcagtga agccgggcca
     1981 gcagattcgt ctcgccgggc agggagggcc gggcatcggc ggcggcgagg cgggcgatct
     2041 ctatctcgaa gtgcagttcc ggccgcaccc gcgctatcgg gtcgagggcc gcgatctcgt
     2101 tctcatgctg ccggtcgcgc cctgggaggc cgcgcttgga gcgagcgttc gcgtgcccac
     2161 tcccgcgggc cccgtcgagc tgaagctgcc gcccggttcg aatgcggggc ggcgtctgcg
     2221 tctgaagggc cggggcctgc ccggcaagac tccgggcgat ctctatgtaa cgatcgaaat
     2281 tgcgctgccg ccggccgaca ccgaagagcg ccgcgagttc taccggcgca tggcccgcga
     2341 attaccgttc gagccgcgca aggggctgga ggcgtagcca tggtgcagaa atccgtcgcc
     2401 atcgttaccg gcatcgtgat cgacgaggag cgcgcactga gtctcgagga acttgcacgc
     2461 gcctgctcca tgcaaagcga atgggtgctc gaactcgtcg cggaaggcat catcgaagcc
     2521 gcctcgcggg aaccggacga gtggcgtttc cccggccgga gcctcgcccg cgcccgcgtc
     2581 gccgcgcgcc tgcaacgcga tctcgacgtg aatctcgccg ggatcgcgct cgcgctcgat
     2641 cttctgcagg aaatcgaaga cctgcgcgcc cggctcggcc tcgccggcga atgatccggc
     2701 gcgcatcccg gatcggcccg gtcggggacc gtgagccgtg aatccgacgc tgcggctgtt
     2761 cgcgttctcc cttcttccgg tcgccgtcct gattggggcc gcagcggcgg gcagcctgcg
     2821 cgcgccgggt ccgcgcttgc gcagcgccgt gctgcatttc gcgggcggag tcatctttgc
     2881 cgtcgtcgcc gtcgaactgc ttcccgacgt ggtgcgggcg cgggccttgc tgccgacggc
     2941 aatcggtttc gcgatcggca ccgcgttgat gctcgccttg cgtgccgcga gtcggcgttt
     3001 cgaacgtcgg gcagatgcgg caacggcggt gttgccgctc ggcctcctcg ccgcggtcgg
     3061 cgtggatctg ctcatcgacg ggctcatgat cggcatcggc tttgccgccg gcgcgcgcca
     3121 gggtgtgctt ctcgccattg cgctggcctt cgagatgctc gcgctcggtc ttgcgctggc
     3181 cgcgacgctc gccaaacgcg gcgccgctcg cggcaaaatc cttgtttgca tcgcggcgct
     3241 cgcgctactc ctcatcgtgg gcgcggtcgc gggcggtgct ccgcttgccg gcgcatccgg
     3301 ccgtgctctc gcgggcgtgc ttgccttcgg ctccgccgcg ctcctgttcc tcgttaccga
     3361 ggagctgctc ggcgaagcgc atgaaacgca agactccgcc gcgctcaccg ccgcgttctt
     3421 tctcggcttt ctggtgcaac tcatcatcga catggcgcac tgacgccaac gtctgtgatc
     3481 cttccgcatg aggtgcgcct cttcgaaacg aagggggccg cgtcttcgcg gggggatgtc
     3541 ctgcagcgta aagatatctg ttcggctcct cgcttctgcg gcaggtccga tacagatttg
     3601 ccgttcccac tggaaatgca aaaaggaccc gagatgcgtc tcggcgtcag aggatcacaa
     3661 gcgtgcgccc ggagccgtag aacgaggagc aatatccgcc aacgcgttca cataggcgtc
     3721 cagcacgatc tcttccgaaa agttctgctc catgtattcg cgtcccgcgc ggcccatcgc
     3781 ggccagcgct tcgggaggga gcgcgagcat gcgttccatg cagcgcgcga gcgaatgcac
     3841 gtcgcggggt ttgcagagaa atccgctctt ctcggccacc accgcatcgc ggcagccggg
     3901 agcatccgtc gtgatgaccg gccgcgccat tgccgccgcc tcgagcaggc tgcgcggcac
     3961 gccctcgcga tagtaagacg gcagcacaac gcaatgcgcc gccgccaacg ccatacggac
     4021 atcgtccagc attcccagat actccacgct gccctccgcc gtccacccgt cgagttcccg
     4081 gcgagagatt gcgctcgaat tatcgtcgtc cacggggccg gccaggcgta cgacaaaatt
     4141 tttgccgcct gtccgcagaa tatgcgccgc ctgcacgagt tcgcgcaggc ctttgtccgc
     4201 gagcagacgc ccgcagaaga gaaatgcaaa cggttcacgc accggcaaag gaacgggggc
     4261 gaatgaatcg agacatatgc cagtcccagg cagc
//
LOCUS       DASUJG010000201         4295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_183563, whole genome shotgun sequence.
ACCESSION   DASUJG010000201 DASUJG010000000
VERSION     DASUJG010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183563"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VFX38_04225"
     CDS             <1..571
                     /locus_tag="VFX38_04225"
                     /inference="COORDINATES: protein motif:HMM:NF021653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HEX5314102.1"
                     /translation="TSIDVNSGACVRGRDFVSTALGVNLEAAEAIARQLRLRNLGGIV
                     VIDLIDMEKAEHGALVLETLRRALARDRARSVVGEISQFGLVEMTRKRNRESLVGAQG
                     EFCASCRGGGRTRTSESVCFEIYRELERLAMNFAAKGYLVLAATKPVELLRGEEASNL
                     AAVCARIGRSVKLQSESVYRPEQFDVVAL"
     gene            555..>4295
                     /locus_tag="VFX38_04230"
     CDS             555..>4295
                     /locus_tag="VFX38_04230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HEX5314103.1"
                     /translation="MSSPSESRPGISRLRKWTIGVAVAVLVIVAIAVGLFFLAATMLP
                     RYHDKIQAEVSTTLGMPVEFSTVSLGWQGWGPAISFRNLRVKNARSGQTVLSARRVEF
                     DFTLPALVHGTHALPSGIRLDAPSLGAVETADGKIRIPGLELQSSSGGPDLRSMLGLH
                     LVIRDGEFRLRLAGAGQPLWRFAPLNLTIGGGRSHAIEFALGLPRQLGGATLHLDGTL
                     DTPEVNIGNWRWSGTYDLPRLRLAPLDRFIPSQYPSVRGTLASSGSFRGSGMSLAAAR
                     GQLKGTKLAAGESSIAKLQTRFMLAAESGILLKLDDTILVEQRGGWKPGEVVIGRDAQ
                     GRLRANVEHLLLAALPPLTGFLPASLQALGERLHKMRPSGTIENIRFAFTPGGSDLDL
                     AAKLNDVGSFAADGAPGFNHLSGRVSLRDGAGGLRIDSPGFTLRMPHIFPHPVPLDRA
                     AGTIAIRYRSSGLNLATDKLAIIGAGGLAGVMRGKIAIPRSGPVTIEIAAKAASIAVV
                     PARERYLPTGLMPRALGEWLRNDLDKGKITGATLAFVGRADKFPFTHGGGRFVVDFGF
                     EGVTLTPGPHWEPLTNLHGEVHFRDAGMRADVAGGGIGGAKIVQATVHLPDLFHPRLA
                     VQAKIAGDSSDFLAFLRASPIGGRLDALDDLAVRGPASADISLHLPIRDIRNFNLSGT
                     LRLDGVTLRYAPLAYTLDDLGGTLNFDQSGPVSGDLQGEILGQPLAITLSRVPSGKRM
                     QISVDGRAPVSVLSGLADYDFSRYAQGDVPFHARASIPLAASGGTFTARLQSDLRGLA
                     LSLPEPVGKSAATSVPFSADASLSDGALELTAIYGKVASACMSFALNGTAAAFRGADI
                     VLGATACHAPAAGLTIRGGWPLLKLGPWFAELPKGKGSAGGGWNMPQALQVDLHFDRV
                     EAFGEHFDNQTIRGRLAAHEIALEFSGDMLAGKLRIPLAPTNEDPIVATLTRAHFSMP
                     GQHRVVVPPAGTAAGSAASAPAAASASPGRVAAKAAAPASAATATITSFRPDQIPPFV
                     LRVTNLELGTAELHDVYIVARRITGGIAMDPIHVGGGALTLDGTMAWIHPPSGNSQGA
                     LHFVANVQHLGALLSGLGLGPVITGHGALSAGIAWHGLPGNSFAKDLLGRVSVDLRDG
                     SISQVSPGAGRLLSLLNLANLPRYLVFNFHNLFGKGFPFSRIYGDYVIDRGIASSKGL
                     RIESSVANIKLTGQVNIAAGTMDQRAEISPNYFGSLPVIAAIV"
BASE COUNT          694 a         1402 c         1413 g          786 t
ORIGIN      
        1 caccagcatc gacgtcaaca gcggcgcctg cgtgcgcggt cgcgacttcg tatccaccgc
       61 gctcggggtc aatctcgagg ctgccgaagc catcgcgcga caactgcggc tgcgcaactt
      121 gggaggcatc gtcgtcatcg acttgatcga catggagaaa gccgaacacg gagcgctggt
      181 gctggaaacg cttcggcgtg cgctcgcccg cgaccgggcg aggagcgtcg tcggcgaaat
      241 ctcgcagttc ggtctggtcg aaatgacccg taaacgcaac cgcgaaagtc tcgtgggcgc
      301 gcaaggcgaa ttctgcgcga gctgtcgtgg aggcgggcgc acgcggacga gcgaaagcgt
      361 ctgcttcgaa atctatcgcg aactcgagcg cctcgcgatg aattttgcgg cgaagggcta
      421 tctcgtgctt gccgcgacga agccggtcga acttctccgc ggggaggagg cttccaacct
      481 cgcggcggtc tgcgcgcgca tcggccggtc cgtgaaattg cagtcggaat cggtctatcg
      541 gccggagcag ttcgatgtcg tcgccctctg aatcgcggcc cgggataagc cggctgcgca
      601 aatggacgat cggggtcgcc gtggccgtgc tcgtcatcgt cgcgattgcg gtggggcttt
      661 tttttctcgc ggccacgatg ctgccgcgtt accacgacaa gatccaggcc gaggtgagca
      721 cgacgctggg aatgccggtg gaattcagca ccgtttcgct cggctggcag ggctgggggc
      781 ccgcaatctc gttccggaat ctgcgggtga aaaatgcccg cagcgggcaa accgtgttga
      841 gcgcgcgcag ggtggagttc gacttcacgc ttcccgcgct cgtacacgga acgcatgccc
      901 ttccctcggg aattcgtctc gacgcgccga gcctcggcgc ggtggaaacg gcggacggga
      961 agatccgcat tccggggctc gaattgcaaa gcagctcggg cggtcctgat ttgcggagca
     1021 tgctcggatt gcatctcgtg attcgtgacg gcgaatttcg tctgcgtctc gccggagccg
     1081 ggcagccctt gtggcgtttt gcaccgctga atctcaccat cggcggcgga cgttcgcatg
     1141 cgatcgaatt cgcactcggc cttccgcgcc aactcggcgg cgctaccctg cacctcgacg
     1201 gaacgctcga cacgccggaa gtcaacatcg ggaattggcg ctggagcgga acctacgatc
     1261 tcccgcgact gcgcctcgca ccgctcgatc gcttcatccc ctcgcaatac ccgtcggtgc
     1321 gcgggacgct tgcgagcagc ggaagtttcc gcggaagcgg catgagtctc gccgcggccc
     1381 gggggcaact gaagggaacg aagcttgccg cgggcgaaag cagcatcgcc aaattgcaga
     1441 cccgattcat gcttgcggcc gaatcgggga ttctgctgaa gctcgacgac accatcctgg
     1501 tcgagcagcg aggcggttgg aagccgggcg aggtggtgat cggcagggac gcacaggggc
     1561 gtttgcgtgc gaacgtggag catctgctgc tcgccgcgct gccgccgctt accggctttc
     1621 tgccggcgag cctccaagca ttgggcgaac ggctccacaa gatgcgtccg agcggaacca
     1681 tcgaaaatat ccgcttcgcg ttcactccgg gaggaagcga tctcgatctc gcggcgaagc
     1741 tgaacgatgt cggcagcttt gccgccgacg gagcgccggg tttcaaccat ctctccgggc
     1801 gcgtcagcct gcgcgacggt gcgggcggct tgcgcatcga ctctcccggt ttcaccttgc
     1861 gcatgcccca catctttcct catccggtgc cgctcgatcg ggcggcggga acgattgcca
     1921 ttcgttatcg ttcgtccggc ttgaacctcg cgaccgacaa actcgcgatc atcggcgcgg
     1981 gcgggctggc cggcgtcatg cggggcaaga tcgcgattcc ccgctccgga cccgtcacga
     2041 tcgaaattgc cgccaaggca gcatcgatcg ccgtcgtgcc ggcgcgggag cgctatttgc
     2101 cgaccggact gatgccccgc gccctcggcg aatggctgcg caacgatctc gacaagggca
     2161 agatcacggg cgcgaccctc gccttcgtcg ggcgcgccga caagttcccc tttactcacg
     2221 gcggcgggcg ctttgtggtg gacttcggct tcgagggcgt gacgcttacg ccgggtccgc
     2281 actgggagcc gctcacgaac ctccacggcg aggtgcattt tcgcgatgcg ggcatgcgcg
     2341 ccgatgttgc cggcggcggg atcggaggtg cgaaaatcgt gcaggcgacg gtgcatcttc
     2401 ccgatctctt ccacccgcgg ctcgcggtgc aggcaaagat cgccggcgac agctcggatt
     2461 tcctcgcctt tctgcgcgcg agcccgatcg gcggaaggct cgatgcgctg gacgaccttg
     2521 cggttcgcgg accggcgagc gccgacattt cgcttcatct gccgatccgg gacatcagga
     2581 atttcaatct ttccggcacg ctgcggctcg acggcgtgac gctgcgctat gcgccgctcg
     2641 cctacaccct cgacgatctc ggcggcacgc tgaatttcga tcagagcggg cccgtcagcg
     2701 gcgatctcca gggcgagatt ctcggtcagc ctctggccat tacgctttca cgcgtgcctt
     2761 cgggaaagcg catgcaaatc tcggtcgacg gccgtgcgcc ggtgagcgtc ttgtcgggat
     2821 tggccgatta cgatttctcc cgctacgcac agggcgatgt gccattccat gcccgggcgt
     2881 cgattccgct ggccgcgagc ggcggaacgt tcacggcccg gctgcaatcc gatcttcgcg
     2941 gacttgcgct cagtctgccg gagccggtcg gcaagtctgc cgcaacttcg gtgccgttct
     3001 cggccgatgc gagcctgagc gacggggcgc tcgagctcac cgccatctac ggcaaagtgg
     3061 cctccgcctg catgagcttt gctctcaatg ggacggcggc agcgtttcgc ggggccgaca
     3121 tcgtgctcgg cgcgaccgcc tgccatgcac ccgcggcggg tctgacgatt cgcggcggct
     3181 ggccgctcct gaagctcgga ccctggttcg ccgaactgcc gaagggaaag ggcagcgcgg
     3241 gcggcggctg gaacatgccg caggcattgc aggtcgatct gcactttgat cgcgtggaag
     3301 ccttcggtga gcacttcgac aatcagacga ttcgcggccg tctcgccgct cacgaaatcg
     3361 cgctcgaatt ctccggcgac atgctcgccg gaaaactgcg cattccgctt gcgccgacga
     3421 acgaggaccc gatcgtcgca accctgaccc gcgcccattt cagcatgccg ggccagcatc
     3481 gcgtggtcgt gccgccggcg ggaaccgccg ccggttcggc cgcctcggcg ccggccgccg
     3541 cttccgcttc gcccgggcgg gtggcggcga aggcggcggc tccggcgagt gccgcgactg
     3601 cgacgatcac gagtttccgt ccggatcaga ttccgccctt cgtgctgcgc gtgacaaatc
     3661 tggaactcgg taccgcggaa ctccacgacg tttacatcgt cgcgcgacgc attacgggcg
     3721 gcatcgcgat ggatccgatt cacgtgggcg gaggggcgct gacgctcgac ggcacgatgg
     3781 cctggattca tccgccgtcg ggaaattcgc agggcgcgtt gcattttgtt gcgaacgtcc
     3841 agcacctcgg cgcactgttg agcgggctgg ggctcggccc ggtgatcacc gggcacggcg
     3901 cgctcagcgc cggcatcgcc tggcacgggc tccccggcaa cagcttcgcc aaggatctgc
     3961 tcgggcgcgt gagcgtggat cttcgcgacg gcagcatttc gcaggtgagc ccgggcgcgg
     4021 gccgtctgct ctcactcctg aatctcgcca atctcccgcg ttatctcgtc ttcaacttcc
     4081 acaacctctt cggcaagggt ttcccgttca gccgcatcta cggcgactac gtgatcgatc
     4141 gcggcatcgc gagcagcaag ggcctgcgca tcgaaagttc cgtcgcgaat atcaagctca
     4201 cgggacaggt gaatatcgcg gcgggcacca tggatcaacg ggccgagatt tctccgaatt
     4261 acttcggttc gctgccggtc atcgccgcga tcgtc
//
LOCUS       DASUJG010000202         4504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_184002, whole genome shotgun sequence.
ACCESSION   DASUJG010000202 DASUJG010000000
VERSION     DASUJG010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184002"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VFX38_04235"
     CDS             <1..331
                     /locus_tag="VFX38_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314104.1"
                     /translation="TIALAFEMFALGLALAATFEARGVARARTLALIAALGLLLVVGA
                     VAGGVPLAGISGRDLAGVLAFGSAALLFLVTEELLSEAHETKDSAVLSGAFFVGFLVL
                     LILAMAG"
     gene            complement(375..803)
                     /locus_tag="VFX38_04240"
     CDS             complement(375..803)
                     /locus_tag="VFX38_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5314105.1"
                     /translation="MKTVRQLLEDKGSEVWSIGPDASVFEALGLMADKGIGALLVLDA
                     GKPVGLLSERDYARKVILKGHSSKELRVGEIMTTEIAWAHPEQSVEECMAIVTERRER
                     HLPVLDDGRIVGLISIGDLVKAIIDHQQFVIEQLEGYISR"
     gene            1032..2765
                     /locus_tag="VFX38_04245"
     CDS             1032..2765
                     /locus_tag="VFX38_04245"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5314106.1"
                     /translation="MPRRYPLLVLLSLFVVAVTAWNCTMQLRFAGLPFALDRVNPTMA
                     RVKSLHGIALPPELKPTDRVHYVDQITRLRMDLLAVIELSSTEPGHTMPLVVTHEDGT
                     KSEVPVQARTLFAEPAFRVSMYLGFAWYLLLCLITLMVLWRGRDRTAWGIALWAIAFE
                     CGMAFYLIPAHDRMVLAFALASQVCFLLARIGFFVMASAIAAPALSERGRRAWLYAFV
                     FSLIVGYGYEVTYVLWFVFDSILIPKIAALIWVVPYAISTAMLLLAHRRAAAAARPRL
                     RWMFWSAVVLVFGILLSNVAFLGYTTSYVIEIACFLIAFAGLLYSVLRHRVVDISFVV
                     NRAIVYSVAVTLIVAIFMVIESFAEKLALSHGESFALELGVPLVIGFSLEAIRKRLEH
                     FGERLFFRRKFENDAALRAFTRQCSYVENPDRLLEQTMRELATHTGTPAVAFYWRDRN
                     DYRRLADGGEARYPERLDGDDRAVVALRADREAMALEEHESVLGSDGGLLPMVVRGEL
                     LGFIVIASRPGERYAPDEREILARLAHEVGSALHALHARENARFISAVAEGNLGGQEL
                     RERARVLMPPA"
     gene            complement(2777..3208)
                     /locus_tag="VFX38_04250"
     CDS             complement(2777..3208)
                     /locus_tag="VFX38_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEX5314107.1"
                     /translation="MGLLRLIGNILWFVLGGIWMGLAWWFVGLVAFVSIVGIPWGRAC
                     FVIGGFSFWPFGRKAVSRRELTGREDFGTGPFGIVGNLIWLVLAGWWLALGHLLSMVL
                     DAVTIIGIPFAIQHLKLAAISLWPIGVRVVPIDLTVPARRN"
     gene            complement(3612..4481)
                     /locus_tag="VFX38_04255"
     CDS             complement(3612..4481)
                     /locus_tag="VFX38_04255"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5314108.1"
                     /translation="MTDRMKAVLVTGAASGIGRATALELDRRGFQVYAGVRRDADAEG
                     LRNAATGDLRPVMLDVTIPEQLRSVRDELERAHGAEGLYALVNVAGIADFGPIEDQSP
                     ERLRKIFDVNFFGVLATIQALLPLIRRARGRIVNVGSVGAHGTIPFGFTVCSSKHAVE
                     ALTSGLRMELSRWGIAAIAIDPSSIATPAADRMVDQAKAAIVGLSEEQRGYYADGLLT
                     MAASMRKQEMAGMPPEGVGKVIAKALSAKRPRARYPVGPHARTIILMSGLLPDRWMDK
                     LILKMVGIRMNDG"
BASE COUNT          708 a         1537 c         1384 g          875 t
ORIGIN      
        1 cacgatcgcg ctcgccttcg agatgttcgc gctcggtctc gcgctcgccg cgaccttcga
       61 agcacgcggc gtcgcacgcg ccaggacgct cgccctgatt gcggcgctcg gccttcttct
      121 cgtcgtcggc gcggtcgcgg gcggcgtccc gctcgccggc atatccgggc gcgacctcgc
      181 gggggttctc gccttcggct cggccgcgct cctcttcctc gtgaccgaag agttgctcag
      241 cgaagctcac gaaacgaaag attccgccgt gctcagcggg gctttttttg tgggttttct
      301 cgttcttctg attctcgcca tggcgggctg aggtcgccac ggcgccaggc cggcatcaag
      361 cgaagcctcg cagctcagcg gctgatgtag ccctcgagtt gttcgatcac gaactgctgg
      421 tgatcgatga tcgctttcac gaggtcgccg atcgaaatca gccccacgat ccgcccgtcg
      481 tcgagtaccg gaagatgccg ctcgcgccgc tcggtgacga tcgccatgca ttcctcgacg
      541 ctctgctccg gatgcgccca ggcgatctcc gtggtcatga tctcgcccac ccgcagctcc
      601 ttcgagctgt ggcccttgag aatgaccttg cgcgcgtaat cgcgttccga gagcaggccg
      661 acgggtttgc cggcgtcgag aacgagcagg gcgccgattc ccttgtcggc catgagcccg
      721 agcgcttcga acaccgaggc atccggtccg atggaccaca cctcgcttcc cttgtcctcc
      781 agcagttgcc gcacggtctt catcgcccgc acctccgctg tccgatcggt gccgctctct
      841 ccaagctagc ctgcgccatc gtgcagcccg agtcaaggcg acgagggccg cgccgatcgg
      901 gaaggtccgc cgatgccgca gcgcggagcg gcttgccgac cgtcgcgcgc ccgacctcgc
      961 tcgccgctgc cttcccggtt gccgccgcgg tgcgctatgc tgcgtcgcgg ccaacgaggg
     1021 agatcgcgaa catgccgcgc cggtatccgc tgctggtgct gctcagcctg ttcgtggttg
     1081 cggttaccgc ctggaactgc accatgcaac tgcgctttgc cggtctgccc ttcgcgctcg
     1141 atcgcgtgaa tccgacgatg gcgcgggtga agagtcttca cggcatcgcg ctgccgcccg
     1201 aactcaagcc gaccgaccgg gtgcactacg tcgaccagat caccaggctg cgcatggatc
     1261 tcctcgcggt gatcgagctt tccagcaccg agcccggaca tacgatgcca ctcgtcgtca
     1321 cgcacgagga tggcaccaag agtgaggtgc cggttcaggc gcggacgctc ttcgccgaac
     1381 ccgcgttccg ggtttcgatg tatctcggtt ttgcctggta tctgctgctg tgcctgatca
     1441 ccctcatggt gctctggcgc ggacgtgacc gtaccgcctg gggaatcgcg ctgtgggcga
     1501 tcgccttcga atgcggcatg gccttctatc tcatacccgc gcacgatcgc atggtcctcg
     1561 ctttcgcgct cgcctcgcag gtctgcttcc tgctcgcgcg gatcggcttt ttcgtcatgg
     1621 cgagcgcgat cgccgcaccg gcactctccg agcgcggccg gcgcgcctgg ctttacgcct
     1681 ttgtgttctc gctcatcgtc ggctatggct acgaagtgac ctatgtactg tggttcgtgt
     1741 tcgattcgat cctgattccg aaaatcgccg cactcatctg ggtggtgccc tatgccattt
     1801 ccacggcgat gctgctactc gcgcatcgcc gcgccgcggc cgccgcccgg ccgcgcctgc
     1861 gctggatgtt ctggagcgcg gtcgtgctcg tcttcggcat tctcctgagc aacgtcgcct
     1921 tcctcggcta cacgacctcc tacgtgatcg agattgcctg ttttctcatc gcgttcgcgg
     1981 ggctcctcta ttcggtgctg cgccatcggg tcgtggacat ctccttcgtc gtcaatcgcg
     2041 cgatcgtcta cagcgtcgcc gtcacgctca tcgtcgcgat cttcatggtg atcgaaagtt
     2101 tcgccgagaa gctggcgctc tcgcacggcg agagtttcgc gctcgaactc ggcgtaccgc
     2161 tggtgatcgg cttttcgctc gaggccattc gcaaacggct cgagcatttc ggggagcggc
     2221 tcttcttccg gcgcaaattc gaaaacgacg cggcgctgcg cgccttcacc cgccagtgtt
     2281 cctacgtgga gaatcccgat cgcctgctgg agcagacgat gcgcgaactc gcaacccaca
     2341 ccggaacgcc cgcggtcgcg ttctactggc gcgacaggaa cgactaccgg cggctggcgg
     2401 acggcggcga ggcgcgttac cccgagcgac tcgacggcga cgatcgcgcc gtcgtcgcct
     2461 tgcgggccga tcgcgaggcc atggcgctcg aagaacacga gagcgtgctc ggcagcgacg
     2521 gggggctcct tcccatggtc gtgcgcggcg aactcctcgg cttcatcgtg atcgcgagcc
     2581 ggccgggcga acgctacgcc cccgacgaac gcgaaattct cgcccgcctc gcccacgaag
     2641 tgggaagcgc gctgcacgcg ctgcatgcgc gcgaaaacgc acgcttcatc tcggccgtgg
     2701 ccgagggcaa tctcgggggg caggaactgc gtgaacgtgc gcgggtgctg atgcctcccg
     2761 cctgagcgcc ttgccgtcag tttcgccgag cgggcaccgt cagatcgatt ggcacgaccc
     2821 gcacgccgat gggccagagc gagatcgcgg cgagcttgag atgctgaatc gcgaagggga
     2881 tgccgatgat ggtgacggca tcgagcacca tgctcaggag atgtccgagc gcgagccacc
     2941 agccggcgag aacgagccag atcaggttgc cgacgatgcc gaaggggccg gtaccgaagt
     3001 cttctctgcc ggtcagttcc ctgcgactca cggccttgcg cccgaagggc cagaacgaga
     3061 atccgccgat cacgaagcag gcgcggcccc aggggattcc gacgatcgaa acgaaggcga
     3121 cgagacccac gaaccaccag gcgagcccca tccagatgcc gccgagcacg aaccagagaa
     3181 tgttgccgat cagccgcaat aggcccatgc ctggctcctt ttctttcaac ccggtcgctt
     3241 cgcctgctca catcgaaaat cggccgaacg catcggcgca gaggcggttg ccgtcacgtc
     3301 catgatggca cccgtcgccg cacaagtcct gcggcgatgt cgggtgccgc cccgtcagat
     3361 gagggacgcc ttgcccgcct tctttgccct tttctcggcc cgtttttcct tgaggttttt
     3421 tgccggtttt ttcttgtctt cctttttcct gtcctgatcc ttgctcatgt ccgcgctcct
     3481 tgcgattgaa aatcgaggcg acgacagcga tgcgggtcgg gggccgatcc ggaaattgat
     3541 tctaaacccg ttttggcgcg gagtggaacg ggcgcggccg tccgcccggg caagccggac
     3601 agggggcggg cttatccgtc attcatccgg atgccgacca tttttaggat gagcttgtcc
     3661 atccagcggt ccggcaacaa accgctcatc agaatgatcg tgcgcgcgtg gggaccaacg
     3721 ggatagcgtg cccgcgggcg ctttgcgctc aaggctttcg cgatcacctt gcccacgccc
     3781 tcgggcggca tgcccgccat ttcctgcttg cgcatggatg cggccatcgt gagcagaccg
     3841 tcggcatagt agccgcgctg ctcttccgac aggccgacga tcgcggcctt cgcctgatcc
     3901 accatccggt cggcggcggg tgtggcgatg gaggaaggat cgatcgcgat cgcggcgatc
     3961 ccccagcgcg acagctccat tcgcaatccc gatgtcagtg cttccacggc gtgcttggag
     4021 gaacagaccg tgaaaccgaa cggaatcgtg ccgtgcgcac ccaccgagcc gacgttgacg
     4081 atgcgcccgc gggcgcggcg aatcagcggc agcaaagcct ggatcgtcgc cagcacaccg
     4141 aagaaattca cgtcgaatat ttttcgcagg cgttccggcg actgatcttc gatcggaccg
     4201 aaatcggcga tacccgccac attgacgagc gcgtacagac cttccgcgcc atgcgcgcgt
     4261 tcgagttcgt cgcgaacgct ccgcagctgt tcgggaatcg tcacatccag catcaccgga
     4321 cgcaagtcgc ccgtcgccgc gttgcgcaga ccttcggcgt cggcatctct tctgacgcct
     4381 gcatacacct gaaacccgcg gcgatccagt tcgagcgccg tggccctgcc gatcccggag
     4441 gccgctccgg tcaccagcac cgccttcatc cgatcggtca tgatgattca cccaggcctg
     4501 cagc
//
LOCUS       DASUJG010000203        10363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_184413, whole genome shotgun sequence.
ACCESSION   DASUJG010000203 DASUJG010000000
VERSION     DASUJG010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 10363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10363
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184413"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VFX38_04260"
     CDS             complement(<1..265)
                     /locus_tag="VFX38_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314109.1"
                     /translation="MKRFTAWLAAGTAAAAFLCAGGLRAAEPAPVNVTMEFDDTVAPA
                     QVEAYEAGIKNFEQCLGQHGFKYAVVALRHVTGDQGQYSYGITV"
     gene            complement(544..634)
                     /locus_tag="VFX38_04265"
     tRNA            complement(544..634)
                     /locus_tag="VFX38_04265"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(598..600),aa:Ser,seq:gct)
     gene            complement(696..917)
                     /gene="csrA"
                     /locus_tag="VFX38_04270"
     CDS             complement(696..917)
                     /gene="csrA"
                     /locus_tag="VFX38_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HEX5314110.1"
                     /translation="MLILTRRSGQTVMIGDGVTVTVLEVKGGRTRLGINAPKDVSVQR
                     EEVYRRGRHAFDSIPEEETMRGKPPVAAL"
     gene            complement(999..2207)
                     /locus_tag="VFX38_04275"
     CDS             complement(999..2207)
                     /locus_tag="VFX38_04275"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019591820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX5314111.1"
                     /translation="MVLKFGGTSLADASCIGRAVALIAAERAAGRKLVVVASAAAGDT
                     DRLLAECRSYATEPDPVAMDLLLATGEQASVARLVLALAARGIPAEAFTGARAGVLTD
                     SAAGKARILRMDCAAVEAVLAAGSVAVVAGFQGVDANGAVTTLGRGGSDTTAVALAAA
                     LGAEECRIYTDVDGVFSADPRLEPAARRLDRIHCEEMLELAGLGSKVLAIESVDYAAQ
                     HAVPLRVLSTFAATAGTAVHHGDDPGAPPVVGVASSAAEAELAAIGLPARPGLAAALI
                     APLAAARVAVDTVLQNEARGGRSDVSFSVPRRDLALARSILAREAGRLGAERLVEDAT
                     VAKVSLVGRGLRSNAPLLARALLALEREGVAVRIVVANETRLSAIVAESSLACAVRAL
                     HREFGLGESP"
     gene            complement(2272..4941)
                     /gene="alaS"
                     /locus_tag="VFX38_04280"
     CDS             complement(2272..4941)
                     /gene="alaS"
                     /locus_tag="VFX38_04280"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008213552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX5314112.1"
                     /translation="MKSNDIRRTFLEFFAARGHRIVPSASLVPANDPTLLFTSAGMVP
                     FKNAFLGRESAPAPRVADVQRCLRAGGKHNDLEQVGYTARHHTFFEMLGNWSFGDYFK
                     REAIAWAWELLTEVWKLPPERLWVTVYHTDDEAWDIWASEIGVPADRIARIGDNQGAL
                     YASDNFWQMADTGPCGPCTEVFYDHDPDGRLGIAGGPPGSDDAEGDRFVEIWNLVFMQ
                     YDRAADGTLAPLPAPCVDTGMGLERIAAVLQGVHSNYDIDIFQHLIRAAAEATGTSDL
                     QSPSLKVIADHIRAAAFLIADGVTPSNEGRGYVLRRIIRRAIRHGYQLGQTKPFFHEL
                     VAPLAEEMGDAYTELKFQSVTIEAAIRQEEVRFAETIGTGMRLLEQAIALGKGEEIPG
                     ATVFKLYDTYGFPVDLTADIARERGIAVDMVGFEHAMDQQRERARAASNFDVELSMGT
                     KANMQFEATVKYDRQDTRFKGYTSTRADAKITTMFNASGRAEQLNVGEKGAVLLDQTP
                     FYAESGGQVGDTGVLLKTGVRFVITDTRERDAGRLHIGQLEQGVLKVGDTVIAEVDAE
                     RRQEIVLNHSATHLLHAALRRLLGTHVTQKGSLVAADRLRFDFSHPQPLTAKDLVEIE
                     DLVNAEIRVNAAAEVREMPYDEAIGAGAMALFGEKYGDNVRVMRFGDFSVELCGGTHV
                     ARTGDIGLFKIVAESGIAAGIRRIEAVTGAGALAFVREEEHRLEEIAQAVKSGREDAA
                     ARVRQLVERARMQERELAELRQRLASGAGGDLAAQAVDVAGHKLVAARLDGADAQTLR
                     TTVDRLKDKLGSAAIVLGAVEDGKVRLAAGVTASLAKRLPASELVSVVAERVGGRGGG
                     RADFAQAGGSEPGKLDEALAAVRDWVDGRLAGG"
     gene            complement(4969..5352)
                     /locus_tag="VFX38_04285"
     CDS             complement(4969..5352)
                     /locus_tag="VFX38_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008547809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX5314113.1"
                     /translation="MRLLLDTHVLLWWPENNPRLGSQARAEIAAATNRVFVSAASGWE
                     IAIKRALGTLEAPAGWAAIVTEKGFTHLPISITHAEHAGALPPHHRDPFDRMLIAQAQ
                     IEGLELVSANPRLARYGVKLLAANV"
     gene            complement(5352..5606)
                     /locus_tag="VFX38_04290"
     CDS             complement(5352..5606)
                     /locus_tag="VFX38_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX5314114.1"
                     /translation="MSETDVPVQVNMHEAKSQLSRLGKLAWCGRRVVIARVGEPYLDL
                     VPHREQPVSRRPGGFEGQIRFAEDFDETPPEVIADFEGDE"
     gene            complement(5638..6123)
                     /locus_tag="VFX38_04295"
     CDS             complement(5638..6123)
                     /locus_tag="VFX38_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEX5314115.1"
                     /translation="MPPRKTEGAPPDAEAIALRLLARREHARGELAAKLRARGVSSAD
                     AAPVLDRLQAAGLLSDARFVEQLLRTRLRQGYGPTRIRRDLAAKGVAPGSEDTALALG
                     DEEWAARAEAIRRRHFGGDLPTEGAERSRQARFLERRGYAAAHIARVLRGDFEADSEL
                     S"
     gene            complement(6104..7135)
                     /gene="recA"
                     /locus_tag="VFX38_04300"
     CDS             complement(6104..7135)
                     /gene="recA"
                     /locus_tag="VFX38_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX5314116.1"
                     /translation="MDDNRKKALAAALGQIEKQFGKGSVMRLGGSETVRDIPVISTGS
                     LGLDIALGVGGLPRGRVVEIYGPEASGKTTLTLQVIAQAQKAGGTAAFVDAEHALDPS
                     YAEKLGVNTEELLVSQPDTGEQALEIADMLVRSSAVDVVVIDSVAALTPRAEIEGEMG
                     DSHVGLQARLMSQALRKLTGNINRSNTMVIFINQIRMKIGVMFGNPETTTGGNALKFY
                     ASVRLDIRRIGSIKKGEEVVGSQTRVKVVKNKVAPPFKKAEFDILYGEGSSREGELID
                     LGAEHGLVDKSGAWYSYEGERIGQGKDNARQFLKDNPEIADRLDKALRDKLLPPKAAR
                     AAPVAAEED"
     gene            complement(7170..7787)
                     /gene="thpR"
                     /locus_tag="VFX38_04305"
     CDS             complement(7170..7787)
                     /gene="thpR"
                     /locus_tag="VFX38_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX5314117.1"
                     /translation="MSRATARQSAAPPSPGLCAAFSTTSEATLRLFFALWPDPDTREA
                     IARATCEAVERAGGRAVPVVNYHLTLAFLGEQPERLLTALARMAAEVEPPRGRLRLDR
                     LGCFEKAGVLWLGPERTPAPLRAWVRTFFAALASFGIAAERRPFAAHLTLARKIRCAP
                     KLPPARAVYWEYEGFALVESVRGREGSEYRVLAEWPAGARGCPVK"
     gene            complement(7711..8214)
                     /locus_tag="VFX38_04310"
     CDS             complement(7711..8214)
                     /locus_tag="VFX38_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005958892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HEX5314118.1"
                     /translation="MAENSDCDDRELAALAKWVGRSLQARHRRMAAAESCTGGWIAKV
                     ITDVPASSNWLDRAFVTYSNAAKVAMLGVSEATLEARGAVSRETACEMAAGALAASEA
                     DVACAVTGIAGPDGGSVEKPVGTVWFAWAERGREPRSALEHFAGDREAVRRAAVARAL
                     RGLLDDE"
     gene            8307..>10363
                     /gene="mutS"
                     /locus_tag="VFX38_04315"
     CDS             8307..>10363
                     /gene="mutS"
                     /locus_tag="VFX38_04315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX5314119.1"
                     /translation="MPAQFRGIIPVADDLAAHTPMMRQYLGIKAEHPDELLLYRMGDF
                     YELFYDDARRAATLLDIALTARGESAGAAIPMAGVPVHAVESYLARLLERGERVVIAE
                     QLGDTAGNGPMHRAVTRVLSPGTVTDEAFLKTKDRVLVAAVANDGASHGFATLDLAGG
                     ELRLAEYPSAAERDASLAAAAPAELLVAEAETAPAGCEAVLRRRPPWHFDVANGRKAL
                     VRQFGVRDLAGYDAEDLASALGAAGALLAYAGETGAAALPQLDRLLRLRPEHSLILDA
                     TTRRNLEIEESLSGRNDLSLLGILDTSATPMGARLLRAWLREPLRDRVILDSRLDAIE
                     EFVASECDASLRERLQGLGDLERILTRAAIGTATPRDLARLRDALARIPAISEALPAE
                     SGGRLAALAARLDPHPGLAAELDRALVENPPAMLKDGEVIAAGYDEALDTARAMDRDA
                     GAGMMALERRERERSGLANLKVGYNRVHGYYLELARRAATRAPAEYTRRQTLKNAERY
                     VTPELKRFENEVLSARERARERERELYGALVARVANGIASLRRLARALGELDLLATLA
                     ERARALDFVRPRFREEPGLTIVGGRHPVVEAAQTNAFVPNDLALDAERRMLIITGPNM
                     GGKSTYMRQVALIALLAHVGSFVPAKQAEIGPLDRIATRIGAADDLARGRSTFMVEML
                     ETAR"
BASE COUNT         1711 a         3602 c         3313 g         1737 t
ORIGIN      
        1 ggacggtgat gccgtaggag tactggccct gatctcccgt gacgtgacgg agtgccacca
       61 ccgcatactt gaaaccgtgc tggcccaggc actgttcgaa gttcttgatt ccggcctcgt
      121 aggcctccac ctgagcgggc gcaacggtgt cgtcgaactc catcgtcacg ttgaccgggg
      181 ctggttcggc cgcccgcagc ccgcccgcac acaagaacgc ggccgcggcg gtgccggcgg
      241 ccagccaagc tgtaaacctc ttcatcgata tgctccatgg gaatgaatat tcccgcaaga
      301 acccgtgttt ctatggttag gatcggggat cccgatcgtc tccgcgtccc tgcggaggcg
      361 catgaatata cgctcagccg ggtgcggcgg caagcccccc ttgcgactat gggcgaaagc
      421 cggaaaccgc cgtcccgtgg agcgagtagg ttgggctgta cgcagcgaag cccaacacca
      481 gcgcgcgcgc tacgacgttg ggcttcacct tgctcagccc aagctgcgcg ccgaaacgaa
      541 atgtggcgga gagagaggga ttcgaaccct cgaagggatt ttggccctta ctcccttagc
      601 aggggagcgc cttcgaccac tcggccatct ctccgatgca aagaatgcgt tggcggctca
      661 acgcggagcg cgtcggcggc agaataccac gggcctcaga gggccgcgac cggcggtttt
      721 ccccgcatcg tctcttcttc gggaatcgag tcgaacgcgt gccggcctcg ccgatacact
      781 tcttcgcgct ggaccgacac gtccttcggc gcattgatgc cgagtcgcgt tcttccgcct
      841 ttgacctcca ataccgtgac ggtaacacca tcgccgatca tgacggtctg cccgcttcgc
      901 cgggtgagga tcagcatggc cacctccttg cgccatacgg tacaggtttc attgtagccg
      961 ccgaaccggt ttctgcaaaa cgcggccggc cggcggcctc aaggggattc ccccaggccg
     1021 aactcgcggt gcagagcgcg gaccgcgcag gcaagggaag attcggcgac gatggccgag
     1081 agccgggtct cgttggccac cacgatgcgc acggcgacgc cttcgcgctc gagcgccaag
     1141 agcgcgcggg cgagcagcgg cgcattggaa cgcaagccgc gcccgaccag cgagaccttg
     1201 gcgacggtcg catcctcgac cagtcgttcg gcgccgaggc gcccggcctc gcgggcgaga
     1261 attgagcgag cgagcgcgag atcgcgccgc ggcacgctga agctcacgtc gctgcgcccg
     1321 ccgcgagcct cgttctggag cacggtgtcc acggcgaccc gggccgcggc gaggggcgcg
     1381 atgagcgcag cggccagtcc cggccgcgcc ggcaggccga tggcggccag ttcggcttcg
     1441 gcggcactgg aggcgactcc gaccaccggc ggcgctccgg gatcgtcccc atgatgaacg
     1501 gcggtgccgg ccgttgcggc aaacgtcgaa agcactcgta gtggcacggc gtgctgggcc
     1561 gcgtagtcca ccgattcgat cgcaagcacc ttcgaaccaa gtccggcgag ttcgagcatt
     1621 tcctcgcagt gaatccgatc gaggcgtcgg gccgcgggtt cgagccgcgg atcggccgaa
     1681 aaaacgccgt cgacatccgt gtaaatgcgg cattcttcgg cacccaatgc ggccgcgagc
     1741 gccactgcgg tcgtatcgga gccgccacga ccgagcgtgg tcactgcacc gttcgcatcc
     1801 acgccctgga agccggcgac gaccgcgacg ctcccggccg cgaggaccgc ctcgacggcc
     1861 gcgcagtcca tccgaagaat cctcgccttg cccgccgcgg agtcggtcag gacgccggcg
     1921 cgggcgccgg tgaaggcttc ggccgggatt ccgcgcgcgg cgagcgcgag caccagccgc
     1981 gcgacgctag cctgctcgcc ggtcgcgagc aggagatcca ttgcgaccgg atcgggttcg
     2041 gttgcgtagc tgcggcattc ggcgagaaga cggtcggtgt cgcccgccgc ggcggaggcg
     2101 acgacgacaa gtttgcggcc ggccgcccgc tcggccgcga tcagcgcgac ggcacggccg
     2161 atgcaggagg catcggcaag cgaggtgcca ccgaacttca gtaccaaacg atccgccgcg
     2221 gcgcgctccc ggagaacagg agtcggcgtc gacgttgcgc ctccgtccgt ttcagccgcc
     2281 cgcaagcctg ccatccaccc aatcgcgaac cgccgcgagc gcctcgtcca gtttccctgg
     2341 ctcgctgcca ccggcctggg cgaagtcggc gcgcccgccg ccgcggccgc cgacccgctc
     2401 ggcgacgacg ctgacgagtt cgctcgcggg caagcgcttg gcaagactcg cagtcacgcc
     2461 ggccgcgagc cggaccttgc catcttccac tgcgccgagc acgatcgccg ccgaaccgag
     2521 cttgtccttc aatcggtcca cggtggtgcg cagcgtttgg gcatccgccc cgtccagccg
     2581 cgccgcgacg agtttgtgtc cggcaacatc cacggcctgt gcagcgagat cgccgcccgc
     2641 gccgctcgca agcctctgcc gaagttcggc gagttcccgt tcctgcatgc gtgcgcgttc
     2701 gacaagttgg cgcacgcgcg cggcggcgtc ctcgcgtccg ctcttcaccg cttgcgcgat
     2761 ttcttcaagc cggtgttcct cttcgcgcac gaaggcaagt gcgcccgcac cagtcacggc
     2821 ctcgatgcgg cggatgccgg ctgcaatgcc ggactcggcg acgatcttga acagaccgat
     2881 gtcgccggtt cgggcgacgt gcgtgccgcc gcacagctcg acggaaaagt cgccgaagcg
     2941 catcacgcgc acgttatcgc cgtacttctc gccgaagagt gccatcgcac ccgcgccgat
     3001 cgcctcgtcg tagggcattt cgcgcacctc ggcggcggcg ttcacgcgga tttcggcgtt
     3061 gacaagatct tcgatctcga cgagatcctt ggccgtcagc ggctgcggat gcgagaagtc
     3121 gaagcgcaga cggtcggctg cgaccagcga acccttctgc gtcacatgcg tgccgagcag
     3181 cctgcgcaat gccgcatgca gcagatgcgt cgccgaatgg ttgagcacga tctcctgcct
     3241 ccgctccgca tccacctcgg cgattaccgt atcgccgacc ttcagcacgc cctgttccaa
     3301 ctgtccgatg tggaggcgac ccgcatcgcg ttcccgagtg tcagtgatta caaagcgaac
     3361 gccagttttt agcaaaacgc ccgtgtcgcc aacttgcccc cctgactcgg cataaaacgg
     3421 ggtttggtct agcaacaccg cccccttttc accgacgttc agctgttctg ctcttccgct
     3481 tgcgttaaac atggttgtga ttttcgcgtc cgctcttgtg cttgtataac ccttaaaccg
     3541 ggtgtcttgg cggtcgtact taactgtggc ttcgaactgc atgtttgcct tagtgcccat
     3601 actgagttcg acatcgaagt tgctggccgc gcgggcacgt tcacgttgct gatccattgc
     3661 atgttcgaag cccaccatat ccacggcgat accgcgttcg cgcgcgatgt cagcggtcaa
     3721 gtccaccggg aaaccgtagg tatcgtagag cttgaagaca gtcgcacccg gaatttcctc
     3781 gcctttgccc aaagcgatcg cctgctcgag caaccgcata ccggtgccga tcgtttcggc
     3841 gaaacgcact tcttcctgcc ggatcgcagc ttcaatcgta acgctttgga acttcagttc
     3901 cgtatacgca tcgcccatct cttcggcaag tggcgcaacg agctcatgga aaaatggttt
     3961 cgtttggccg agttggtagc cgtggcggat ggcgcggcgg atgatgcggc gcagcacgta
     4021 gcctcggcct tcgttggaag gcgttacgcc gtcggcgatc agaaaggccg ccgcacggat
     4081 gtgatcggcg atcaccttga gcgacggcga ttgcaagtca ctcgtaccgg tcgcctcggc
     4141 cgccgcgcga atgaggtgct ggaaaatatc tatgtcgtag tttgaatgca cgccctgcaa
     4201 taccgcggcg atgcgctcga gccccatgcc ggtgtccacg cagggtgcgg gcagcggcgc
     4261 cagcgtgccg tcggcagcgc gatcgtactg catgaagacg agattccaga tctcgacgaa
     4321 gcggtcgccc tcggcatcgt ccgaacccgg tggaccaccg gcgataccga gtcggccgtc
     4381 aggatcgtga tcgtaaaaga cttcggtgca cgggccgcag gggccggtat cggccatctg
     4441 ccagaagttg tccgatgcgt acagcgcgcc ctgattgtcg ccgatgcgtg caatgcgatc
     4501 ggccgggacg ccgatctcgc tcgcccagat gtcccaggcc tcgtcgtcgg tgtgatacac
     4561 cgtgacccag agacgctccg gcggcaactt ccagacttcg gtcagcaact cccaggccca
     4621 ggcgatcgcc tcgcgcttga agtaatcgcc gaaggaccag ttgccgagca tctcgaagaa
     4681 ggtgtgatgg cgcgcggtgt agccaacctg ttcgagatcg ttgtgtttgc ctccggcgcg
     4741 caggcagcgc tgcacgtccg cgacgcgcgg cgccggcgcg ctttcgcggc ccagaaacgc
     4801 gtttttgaac ggcaccatgc cggcgctggt gaagagcagc gtcggatcgt tcgccggcac
     4861 cagcgacgcg gaaggcacga tacggtgccc gcgcgcggcg aaaaattcca gaaacgtccg
     4921 gcggatatcg ttgcttttca tcggctcagg atacccgaga cggcgcgatc aaacgttggc
     4981 ggcaagcagt ttgacgccat agcgcgccag gcgcggattg gcactcacga gttcgagtcc
     5041 ctcgatttgt gcctgagcaa tcagcatgcg atcgaaggga tcgcggtgat gcgggggaag
     5101 tgcacccgca tgttcggcgt gggtgatgct gattggcagg tgcgtgaagc ccttttcggt
     5161 tacgatcgca gcccagcctg ccggcgcctc gagtgtaccg agcgcgcgtt tgatcgcaat
     5221 ctcccacccg cttgccgcac ttacaaaaac ccgattggtg gcggctgcga tttcggcccg
     5281 ggcctgcgaa ccgagccgag gattgttttc aggccaccac agcaggacat gcgtatcgag
     5341 gagcagccgc atcactcgtc gccttcgaaa tcggcgatca cctccggcgg cgtttcgtcg
     5401 aaatcctcgg caaatctgat ttggccctcg aagccccccg gccggcgcga aaccggttgc
     5461 tcgcgatgcg gaacaagatc cagatacggt tcgccgaccc ttgcgatcac cacccgccgt
     5521 ccgcaccacg ccagcttgcc cagccgcgag agctgggatt tggcttcgtg catgttgacc
     5581 tgcaccggta cgtcggtttc cgacattgca tccttcccaa cgttagctga gctaaatcta
     5641 gcttaattca gagtcggcct caaagtcgcc gcgcagaacg cgcgcgatgt gggccgcggc
     5701 gtagccacgc cgctcgagga agcgcgcctg gcgggaacgc tcggcccctt cggtcggcag
     5761 atcgccgccg aaatggcggc ggcgaattgc ttccgcccgc gccgcccatt cctcgtctcc
     5821 gagggcgagc gcggtgtcct cgctccccgg agcgacaccc ttcgcggcca ggtcccggcg
     5881 gatgcgcgtc ggcccgtaac cctgcctcag ccgcgtacgc aacagttgct cgacgaagcg
     5941 cgcatcggac agcaatcccg ccgcttgcag ccgatcgagc acgggcgcgg catccgccga
     6001 cgacacgccg cgggcgcgaa gcttcgccgc gagttcgccg cgcgcgtgtt cgcgccgcgc
     6061 gagcagccgc agcgcgatcg cctcggcgtc gggcggcgcg ccttcagtct tcctcggcgg
     6121 caaccggcgc ggcccgcgca gccttcggcg gcagaagttt gtcgcgcaag gccttgtcga
     6181 ggcgatcggc aatctccgga ttgtccttga ggaactggcg cgcgttgtcc ttgccctggc
     6241 cgatgcgttc accctcgtag ctgtaccagg ctccggactt gtccaccagc ccgtgctcgg
     6301 cgccgaggtc gatcagctcg ccttcgcgtg aactaccctc gccataaagg atgtcgaact
     6361 cggccttctt gaacggcggc gcaaccttgt tcttcaccac cttcacgcga gtttgactgc
     6421 cgacgacttc ctcgcccttc ttgatcgagc cgatgcggcg gatgtcgagg cgcaccgagg
     6481 cgtaaaactt gagcgcattg ccgccggtcg tcgtctccgg attgccgaac atcacgccga
     6541 tcttcatccg gatctggttg atgaagatca ccatcgtgtt cgagcggttg atgttcccgg
     6601 tgagtttgcg cagggcctgg ctcatcagcc gcgcctggag cccaacgtgc gagtcgccca
     6661 tctcgccctc gatttcggcc cgcggcgtaa gcgctgcgac cgaatcgatc accaccacgt
     6721 ccactgcgct cgaacgcacc agcatgtcgg cgatttcgag cgcctgctca cccgtgtcgg
     6781 gctgcgacac cagaagctcc tcggtattca cgccgagctt ctcggcatag ctcgggtcga
     6841 gcgcatgttc ggcatccacg aaggccgcgg tgccgcccgc cttctgcgcc tgcgcgatta
     6901 cctgcaaggt gagcgtggtc ttgccggaag cttccggccc gtagatttcc accacccgtc
     6961 cgcgcggaag ccccccgact ccgagcgcga tgtcgagccc cagcgaaccg gtcgaaatca
     7021 ccgggatgtc gcgcacggtc tccgagccgc cgaggcgcat caccgagccc ttcccgaact
     7081 gcttttcgat ctgtccgagc gccgcagcca gcgccttttt gcgattgtcg tccatccctc
     7141 gcctcatgat tgaaaaaagt ccgcagccat tatttcacag ggcatccgcg cgcgcctgcc
     7201 ggccattcgg cgagcacgcg gtattcgctt ccctcgcggc cgcgaacgct ttcgaccagc
     7261 gcgaagccct catattccca gtacacggcg cgggcgggcg gcagcttggg cgcgcaacgg
     7321 atcttgcgcg ccagcgtgag atgcgccgcg aaggggcgcc gctcggcggc gatgccgaag
     7381 gaggcaagcg cggcgaagaa tgtgcgaacc caggcgcgca atggcgcggg cgtgcgctcc
     7441 ggcccgagcc agagcacgcc cgccttctcg aaacagccga gccgatcgag ccgcaggcgg
     7501 cctcgcggcg gttcgacttc ggccgccatc cgggccaacg cggttagcag tcgctccggc
     7561 tgctcgccga gaaaggcgag ggtcaggtga tagttcacga ccggcaccgc ccgcccgccg
     7621 gcgcgctcca ccgcctcgca ggtcgcccgc gcaatggctt cgcgcgtgtc gggatcgggc
     7681 cagagtgcga aaaagagacg cagtgtcgcc tcactcgtcg tcgagaaggc cgcgcaaagc
     7741 ccgggcgacg gcggcgcggc ggactgcctc gcggtcgccc gcgaaatgtt cgagcgcgct
     7801 gcgcggctcc cgtccccgct cggcccaggc gaaccagacg gtgccgacgg gcttctccac
     7861 cgagccgcca tcgggcccgg cgatgccggt caccgcgcag gccacgtcgg cttcgctcgc
     7921 ggcgagcgcc cccgcggcca tctcgcaggc ggtctcgcgc gataccgcgc cgcgggcctc
     7981 gagcgtcgcc tcggaaacgc ccagcatggc gaccttcgcc gcattgctgt aggtgacgaa
     8041 ggcgcgatcc agccagttcg aactcgccgg cacgtcggtg atgaccttgg cgatccagcc
     8101 gccggtacag gactccgccg ccgccatgcg ccggtgtcgc gcctgaagcg accgcccgac
     8161 ccatttcgcg agcgccgcaa gctcccgatc gtcgcagtcc gagttttcag ccatgcgctc
     8221 gttccgccac ggagcgacag agtttcgatt gtagcgactg cgacggcaga ctcgccgcac
     8281 gatcgcattg cgccgggctg ctagtattgc cggcgcagtt tcgtggaatc attccggtgg
     8341 ccgacgatct cgccgcgcac acgccgatga tgcgccagta cctcggtatc aaggccgagc
     8401 atcccgacga attgctgctc taccggatgg gcgatttcta cgaactcttc tacgacgacg
     8461 cgcgccgtgc cgcgacgctc ctcgacatcg cccttaccgc gcgcggcgaa tcggccgggg
     8521 cggcgattcc gatggcgggc gttcccgtgc atgcggtgga aagttatctc gcgcgcctgc
     8581 tcgaacgcgg cgagcgggta gtgattgccg agcagctcgg cgatacggcg ggcaacgggc
     8641 cgatgcaccg cgccgtgacc cgcgtccttt cccccggcac cgtgaccgac gaggcgtttc
     8701 tcaagacgaa ggaccgcgtg ctcgtcgcag ccgtcgcaaa cgacggcgcg agccatggtt
     8761 tcgcgacgct cgatctcgcc ggcggcgagc tgcgcctcgc cgaatacccg agtgccgcgg
     8821 aacgcgatgc gtcgctcgcc gccgccgctc cggcagaact tctcgtcgcc gaggccgaaa
     8881 ccgcgcccgc gggatgcgag gcggtgctgc ggcggcgccc gccctggcac ttcgatgtcg
     8941 cgaacggccg caaggcgctg gtccggcagt tcggggtgcg ggatcttgcg ggctacgacg
     9001 ccgaagacct ggcatcggcg ctcggtgccg ccggcgcgct gctcgcctac gcgggcgaaa
     9061 ccggcgcggc ggcgctcccg cagttggacc ggctgctgcg attgcgcccc gagcacagtt
     9121 tgattctcga tgcgaccacg cgccgcaacc tcgaaatcga agaatccctt tcggggcgaa
     9181 acgatctgag cctcctcggc atcctcgaca ccagcgcgac gccgatgggc gcgcgactgc
     9241 tgcgagcctg gttgcgagag cccctgcgcg atcgcgtgat cctcgactcg cgcctcgatg
     9301 cgatcgagga gttcgtcgca agcgagtgcg acgcgtcgtt gcgcgaacgc ctgcagggtc
     9361 tgggcgatct cgaacgcatc ctcacccgcg ccgccatcgg cacggcgaca ccccgcgatc
     9421 ttgcgcgcct gcgcgacgcg ctcgcgcgca tcccggcgat ctcggaagcc cttcccgcag
     9481 agtcgggagg gaggctcgcc gcgctcgcgg cgcgactgga cccgcatccc gggctcgccg
     9541 ccgaacttga ccgcgcgctc gtcgaaaacc cgcccgcgat gctcaaggac ggcgaggtca
     9601 tcgccgccgg ttacgacgag gcgctcgata ccgcccgtgc catggatcgc gacgcgggag
     9661 cgggcatgat ggcgctggag cggcgcgaac gcgagcgcag cggtctcgcc aatctcaagg
     9721 tcggctacaa ccgggtgcac gggtattacc tcgaactcgc acgccgcgct gccacccgtg
     9781 cgcccgccga atacacccgc cgccagaccc tcaagaatgc ggagcgctac gtcacccccg
     9841 aactcaaacg cttcgagaac gaagttctct cggcaaggga gcgcgcccgc gaacgagaac
     9901 gcgagttgta cggcgcgctc gtcgcgcgtg tggcgaacgg aatcgcaagc ctgcgccggc
     9961 tcgcccgcgc gctcggggaa cttgatctgc tcgcgacgct cgccgaacgc gcccgcgccc
    10021 tcgacttcgt gcgcccgcga ttccgcgagg agccggggct cacgatcgtc ggcggacgcc
    10081 atccggtggt cgaggccgcg cagacgaacg ctttcgttcc caacgatctc gcactcgatg
    10141 ccgaacggcg catgctgatc atcaccggtc ccaacatggg cggcaaatcc acttacatgc
    10201 gccaggtggc gctgatcgcg ctcctcgccc acgtcgggag cttcgtcccg gccaagcagg
    10261 cggaaatcgg ccccctcgat cgcatcgcaa cccgaatcgg cgccgccgac gatctcgccc
    10321 gcggacgctc caccttcatg gtcgagatgc tggagacggc gcg
//
LOCUS       DASUJG010000204         9798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1773821, whole genome shotgun sequence.
ACCESSION   DASUJG010000204 DASUJG010000000
VERSION     DASUJG010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9798
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1773821"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /gene="cydB"
                     /locus_tag="VFX38_04320"
     CDS             <1..632
                     /gene="cydB"
                     /locus_tag="VFX38_04320"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX5314120.1"
                     /translation="GLMAVALSLYQAGAMVSLRSEGVILSRAKRMMIRAAAAAFVIFT
                     AGGIWLSQLSGYAPSGTVNPAMAANPLASTATMAKGLWLANFFAQPALFVVPILVYAG
                     LLAGLLFAHRNHPRLAWCSGAIAWAATIGTVGAAMFPMLAPSYANVNQSLTVWNSTSS
                     LRTLSWMLGFTLVFIPLIVFYTCWAFRVMRGKVRPEDIAERDRKGEESY"
     gene            complement(654..2234)
                     /gene="glgA"
                     /locus_tag="VFX38_04325"
     CDS             complement(654..2234)
                     /gene="glgA"
                     /locus_tag="VFX38_04325"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEX5314121.1"
                     /translation="MTVRGKYVLEGDGDGSRRAGSIAALRSGAEPAEGRRGAALKVLM
                     VSAECYPLAKTGGLADVCTGLALSLLKKGLRLRLLVPGYDCVFAGLRRVLPVAEIAGL
                     PAPAKLLASRIPGSSLRLWILDSPELFRRRGIYQNEAGEDWPDNFRRFAALGMAAARI
                     AEGRVAGLRWRPDLVHCHDWCAGLTPLLLAGGGVSRPASIFTIHNASFQGNFPLSHAR
                     DLGLPANTLGPEGAEFYGRFSFLKAGVRYADAVSTVSPRYSREIRSPEFGCGMEGLFA
                     AKGENLVGILNGVDTQLWNPATDRWISRNYSAAHPAGKARCKLDLQRRLGLDADPRAP
                     LGIFVCRLTAQKMADILLGVLPALLALHPRLQFTLHGCGERRFETGFTALAARFPGRV
                     AVRIGYDEALAHRLQAGGDFLFHGCRFEPCGLVQQYAMRYGTLPIVRRTGGLADTVSD
                     RAGGRETGFFFEEPTANALAAAAENCLRLYAGDRLAWKRRQRNAMEEQPGWESSSQAY
                     LRLYQRCITERRAIAGRQ"
     gene            complement(2306..3271)
                     /locus_tag="VFX38_04330"
     CDS             complement(2306..3271)
                     /locus_tag="VFX38_04330"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX5314122.1"
                     /translation="MLIQNDLYPTRGDQERLIERKDPILYGWDHPNKCLSESQLTNFE
                     QRGFVLVPNAFSPAEVALLKAEVQRVKRQAGAGRNEIWRDAVPAGERESLLAPERASR
                     LLRGLACERRILDGVMQIVGGPVCLHRARVHIEDGSHGTAYPWHSEFETWHAEDGIPR
                     MRGVEAWVLLERSEAEGGALCMLSKSHHIYAACVANGDGAPRGAAGKREVPRPSFEAL
                     LRLRRFGGLAWAQGEAGTLVLCDSNLMYGSSPGPKGRARSTVMFSYNSLENLPAERPF
                     ASREFRPAPIVSMPFARLKGEFFDALVASEGSQRIKPAARARVSA"
     gene            complement(3451..3690)
                     /locus_tag="VFX38_04335"
     CDS             complement(3451..3690)
                     /locus_tag="VFX38_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX5314123.1"
                     /translation="MNQDQFQGQWKQMKGNIRESFGKLTDDDVEQIAGKRDQLVGKLQ
                     ERYGDSREEAERRVHDYEGTLRMAAPPSSGVKPSH"
     gene            4110..4535
                     /locus_tag="VFX38_04340"
     CDS             4110..4535
                     /locus_tag="VFX38_04340"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX5314124.1"
                     /translation="MVVAAGPHAQALLPGSRKPLRSAAALIGEAVRDERGKRIGAIED
                     LLLDVSGERVAYLVLAIGGMLGIGSRLLAIVPDMLERNRSDDGLRLKVTRRALDEAPD
                     YDRTRWMDLAASPQAGAILRLRAMRSGASIPRSNLPQAR"
     gene            4688..4867
                     /locus_tag="VFX38_04345"
     CDS             4688..4867
                     /locus_tag="VFX38_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314125.1"
                     /translation="MKRLLPQHKPFGRLWTETLSERWKREFRLRRIDARGMEPIRVRV
                     RLLRERRHDRPIPPQ"
     gene            complement(5292..6446)
                     /locus_tag="VFX38_04350"
     CDS             complement(5292..6446)
                     /locus_tag="VFX38_04350"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX5314126.1"
                     /translation="MFRLIVAFGIFAAGGAALAAVPVPPPPPLSAASYVLMDYRTGQI
                     LAARDADAHRAPASTTKLMTAYVVFQDLAAGHIRLDTPFTVSENAWREIGSRMFIKLG
                     SQVSVNELLQGMLIPSGNDAAMALAEGVAGTEAGFVNMMNADAKKLGLANTHYEDPAG
                     LAGDGQYTSAGDLARLARRIIRDYPQYYHYFGQKDFTWNKIHQTNWNKLLWLDSDADG
                     LKTGYTSDAGYCLVASVKRGNRRMIAVVMGVPGVSKTDNMANYRHLAKVTESLVDYGF
                     RFFQTRKMEDAGKALAKIRVWKGEDKYVEAGLAAPLYVTAALGEFPDMKISTHVDANV
                     KAPVAAKQTVGELTVSADGKTVARAPLVALKPVAKGGIWAWIRDTVLGWF"
     gene            6536..7093
                     /locus_tag="VFX38_04355"
     CDS             6536..7093
                     /locus_tag="VFX38_04355"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX5314127.1"
                     /translation="MLRDSESRWGSLSQFFHWSVAALIFALIALGWLVRLTPLSPGQI
                     TLFYWHKSLGMLALALVLVRLGWRAGNRPPPLPGGLPRWEAPLARTTHVALYLLILLM
                     PVSGWLVNSASGIPFKIFWVLPLPAIAPVSAGLEHAFELLHLASFSALALLLVGHIGA
                     ALRHHFLLHNDVFLRMLPRGRRRTR"
     gene            7090..7656
                     /locus_tag="VFX38_04360"
     CDS             7090..7656
                     /locus_tag="VFX38_04360"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX5314128.1"
                     /translation="MMRMALPLLAAILLAGTAGAAATAPGWHSVADGSRIGFVARWQG
                     TPVPGSFEQFSVQARFDPNHPAGGEITVDIASASVHAQSADVTRALRAPAWFDVQHYP
                     QASFRSQKILASGAHSVRVSGTLRIKGQSRQVTFPLNFASKGSRLILDGSLDLRRSDF
                     GIGSGQWAGGKIIAIPVTVDFHVVLDPD"
     gene            7657..8388
                     /locus_tag="VFX38_04365"
     CDS             7657..8388
                     /locus_tag="VFX38_04365"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX5314129.1"
                     /translation="MIRRFVLVAGLGTILGACAAPKPPAPSAAAPKPAISAAYREDAR
                     AGRAVYRLDAAASRVEILVGRAGAFASAGHLHVIEVGRLEGYARLANGGGRADLVFPL
                     AAMVVDPPAARSALGGAYAEPLGESQREGTRANMLGAKGLDAARYPWAHLAITAPATA
                     SGSVPITAAISLHGITRQLSAKTALAFDAKHLQADGELHLRQSNFGITPYSVLLGALK
                     VADTLDIRYHLRFTRWCPVPGPTSC"
     gene            8382..9101
                     /locus_tag="VFX38_04370"
     CDS             8382..9101
                     /locus_tag="VFX38_04370"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HEX5314130.1"
                     /translation="MLNPDLDVAARAAEFAHRRRVLVPQAFTAETAERLARCLREEVP
                     WGLATRSGGESRWLRTEELRTLRANPAEWRKLLEEVHNEARSGYQFFYNSYQMIAAYK
                     ERLDPQLYLHRFLEFLNSPPMIEFARRVTGMADIAKADAQATRYLPGHFLRRHDDVYR
                     EGERDSRRAAYVINLTRGWQADWGGLLQFLDAAQNVEETWTPDYNVLAIFAVPTWHCV
                     SCVAPFATEPRLAITGWFRTH"
     gene            9289..>9798
                     /locus_tag="VFX38_04375"
     CDS             9289..>9798
                     /locus_tag="VFX38_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314131.1"
                     /translation="MQPMARNKDTKRLEARTPCAETILAAVHEHPIMRNLWIGMLFAG
                     VSACAQAPATATITPASDASPARGALQNKARAAVAASSVSFYVEDGDLPPYELIKRLS
                     VSGIGGCTNATKTMKLLIEAKTQAAALGANGVLLQLHGTMSSGMTIVKGNRSVFSVAQ
                     YAGSAPGGST"
BASE COUNT         1642 a         3322 c         3146 g         1688 t
ORIGIN      
        1 gcggcctcat ggccgtcgcg ctctcgctct atcaggcggg cgcgatggta tcgctgcgct
       61 ccgagggagt gattctctcc cgcgccaaac ggatgatgat ccgggccgcc gccgccgcgt
      121 tcgtgatctt caccgcgggc ggcatctggc tcagccaatt gagcggttat gcgccgagcg
      181 ggaccgtgaa tcccgcgatg gcggccaacc cgctcgcgag cacggcaaca atggccaaag
      241 gtctgtggct cgcgaatttc ttcgcgcaac cggcgctttt cgtcgtgccg atcctcgtat
      301 acgcgggact gctcgcgggg ctcctgttcg cccaccgcaa tcaccccagg ctcgcgtggt
      361 gctccggcgc aatcgcctgg gcggcgacca tcggcacggt gggtgcggcg atgttcccga
      421 tgctcgcgcc gtcctacgcg aatgtcaacc agagtctcac ggtgtggaac tcgacctcgt
      481 cgctgcgcac gctctcatgg atgctcggct tcacgctcgt tttcattccg ctgatcgtct
      541 tctacacctg ctgggcgttt cgcgtgatgc gcggcaaggt tcgcccggag gacatcgccg
      601 agcgcgaccg caagggcgag gaaagttact agcagggtgc cgagctaact ctgctactgc
      661 cggccagcga tcgctcgccg ttcggttata cagcgttggt agagccgcag atatgcctgc
      721 gacgatgact cccagcccgg ctgctcttcc attgcgttgc gctgccgccg cttccacgcg
      781 aggcgatcgc ccgcgtacag tcgcagacag ttctccgccg ccgccgcgag cgcattcgcc
      841 gtgggttcct cgaagaagaa tccggtctcg cgtcccccgg cgcggtcgct cacggtgtcg
      901 gcgagcccgc cggtgcgccg cacgatggga agcgttccat accgcatcgc gtattgctgc
      961 acgagtccgc agggctcgaa ccggcaaccg tgaaagagaa agtcgccgcc ggcctgaagc
     1021 cgatgcgcaa gagcctcgtc gtagccgatc cgtacggcca cccgtccggg aaagcgtgcg
     1081 gccagcgcgg tgaatcccgt ttcgaaacgg cgctcgccgc agccgtgcag cgtgaactgt
     1141 aatcgcggat gcagcgcgag cagcgccgga agcacgccaa gcaggatgtc cgccatcttc
     1201 tgcgcggtca gccggcacac gaagatgccg agcggcgcgc gcggatcggc gtcgagaccg
     1261 aggcggcgct gcagatccag cttgcagcgt gccttccccg ccggatgcgc ggcggagtaa
     1321 ttccgactga tccagcgatc ggtcgccgga ttccacagct gggtatcgac gccgttgaga
     1381 atgccgacga gattctcgcc cttggccgcg aacaatcctt ccatgccgca gccgaattcg
     1441 gggctgcgga tttcgcgcga atagcggggg ctcaccgtgc tcaccgcatc ggcgtagcgc
     1501 accccggcct tgagaaatga gaatcgcccg tagaactcgg cgccctcggg gccgagcgta
     1561 ttcgcgggca gcccgagatc gcgggcgtgt gaaagcggga aattcccctg aaaggacgca
     1621 ttgtgaatgg tgaagatgct cgccggccgc gatactcccc cgcctgcgag aagcagcggc
     1681 gtgagcccgg cgcaccagtc gtggcagtgc acgagatccg gacgccagcg cagccctgcg
     1741 acccggcctt cggcgatgcg cgcggccgcc atcccgagcg cggcaaaacg ccggaaattg
     1801 tccggccagt cctcgcccgc ttcgttctga taaatcccgc ggcggcgaaa gagctccggg
     1861 ctgtcgagga tccagagacg cagcgaactg cccgggatgc ggctcgcgag gagtttcgcg
     1921 ggcgcgggaa gtcccgcgat ttccgcaacc ggcaggacac ggcgcaggcc ggcgaatacg
     1981 caatcgtagc ccggcaccag cagccgcaaa cgcaagccct tcttcagcag cgaaagcgca
     2041 agtccggtac agacgtcggc cagtccgccc gtcttggcca gggggtagca ctcggccgag
     2101 accatcagca ctttcagcgc ggcgccacgg cgtccctccg ccggctcggc cccggatcgg
     2161 agcgcggcga tcgatccggc tcgccgcgaa ccgtccccat caccttcgag gacgtacttt
     2221 cctcgaaccg tcacctcctc aactcgcggc atggcgcgac gcagcggctg gctccgcgcg
     2281 gccgcgggcg ctcacccggc ggggctcatg cgctcactct tgcgcgagct gccggcttga
     2341 ttcgctggct gccctcgctc gcgacgagcg catcgaaaaa ttcacccttg agccgggcga
     2401 agggcatgct cacgatcggc gccggacgaa attcgcggct cgcgaagggc cgctccgccg
     2461 gcaggttctc gaggctgttg taggagaaca tgaccgtcga gcgcgccctc cccttcggtc
     2521 ccggggaaga accgtacatg agattgctgt cgcagagaac gagcgtgccg gcttccccct
     2581 gcgcccaggc gagcccgccg aaacgacgca gacgcaggag cgcttcgaag ctcggccgcg
     2641 ggacttcgcg cttgcccgcg gcgccgcgcg gcgcaccgtc cccattcgca acacaggccg
     2701 catagatgtg gtgcgacttg gacaacatgc acagtgcccc cccttcggct tcactgcgct
     2761 cgaggagaac ccatgcctcg accccgcgca ttctcgggat tccgtcctcg gcatgccagg
     2821 tctcgaattc ggaatgccag gggtaggcgg tgccatggct gccgtcctcg atatggaccc
     2881 gcgcgcgatg gagacatacc gggccgccca cgatctgcat gacgccgtcg aggatccggc
     2941 gctcgcaggc gaggccgcga aggagcctgc tcgcgcgctc gggggcgagg agcgactcgc
     3001 gctcaccggc gggaacggcg tcgcgccaga tttcgttgcg gcccgcgccc gcctgccgtt
     3061 tgacccgctg tacctcggcc ttcaacagcg cgacctccgc aggggagaag gcattcggaa
     3121 cgagaacgaa gccgcgctgc tcgaagttcg tcagctgcga ttcgctcagg catttgttgg
     3181 ggtgatccca gccgtagaga atcggatcct tgcgttcgat caagcgctcc tgatcgccgc
     3241 gggtgggata gagatcgttc tggatgagca tggccgtacc tccgggtccc ttggtatgca
     3301 tggggcaaga gtaatgagcc cgcggatgca attccgtgcg ctgacgcacg ccccgcggct
     3361 cgccgacccg gccccgatcg aacgaggtcc gcgccccgcc ttccgagggc ggcggcgcgg
     3421 gcccgctccg ctcgattccg cgaccgcggc tcagtggctg ggcttgactc cgctgctcgg
     3481 cggcgccgcc atgcgcagcg tcccttcgta gtcgtgcacc cgccgctccg cctcctcccg
     3541 gctgtcgccg taacgctcct gcagtttgcc gacgagttga tcgcgctttc cggcgatctg
     3601 ttcgacgtca tcgtcggtca acttgccgaa gctctcgcgg atgtttccct tcatctgttt
     3661 ccactgtccc tggaactgat cctgattcat gtcaatctcc ttacgcgtac tgaaaacacc
     3721 gacaccgcgg ctgcggtttg tcggaccatc cgtcagtttc ttggagtttc acctcgtcaa
     3781 taggttcaag cacgcgggcc ttgcgcgaag cccgtgccgg cgcgtttttc gacgtgtgcg
     3841 atggcgcaca gactgcgctc cgttttcggg ttcatcgtaa acgcagggcg atggccctcg
     3901 tgcagacctc gcaggtcgcg gacgtctgca aggaaaccgc aagaggacgc aacgatgagg
     3961 caggaaaact acatttccaa accacgcaag gcgcggttcg acgacaagtc acaaccccgc
     4021 gatcgggacc gggatttcgc ggatcgggac cgaaggaaag cggagcgacg caagctccgt
     4081 cgggacgggg cgagaatgcg gcagatcgaa tggtggtagc cgccggcccg catgcacagg
     4141 ctctcctgcc gggatcccgc aagccgttgc gctcggccgc ggcgctgatc ggcgaggcgg
     4201 ttcgcgacga gcgcggcaag cggatcggcg cgatcgaaga tctgctgctc gatgtttccg
     4261 gcgagcgcgt cgcctacctg gtccttgcga tcggcgggat gctcgggatc ggaagcagac
     4321 tcctcgcgat cgttcccgac atgctcgagc gcaaccgaag cgacgacggc ctgcgactca
     4381 aggtcacgcg ccgcgcactc gacgaggcgc ccgattacga ccgcacgcgc tggatggacc
     4441 ttgccgcctc cccgcaggcc ggcgcgatcc tgcgcttgcg cgcaatgcgc agcggcgcaa
     4501 gcataccgcg gagcaatctc ccgcaagcac gctgagaagc accctgatgt gcgacgtcga
     4561 cacgacgccg cacgtcacca tttctgttct ttgcgaacgt ttgccaaacc caacactctc
     4621 actaattgca gatgcaacgg cgtgatgcct cctgcgtccg caacccgctc gaaccggaga
     4681 gatgacgatg aaacgattat tgccgcaaca caagcctttc ggaagactct ggaccgaaac
     4741 cctgagcgaa cgctggaagc gggagtttcg cctgcggcgc atagacgcgc gcggcatgga
     4801 accgatccgc gtgcgcgtgc gcctgctgcg ggagcgccgg cacgaccgcc ccattccccc
     4861 gcagtgagtc gcaggtcgcg cagcggcgct cgccggggca gtcagcgatt tcattgcgag
     4921 cgtttcacgg ccgcgggatg atgaacttgc ccgcgcggat ttccgcgcgg gaagtcggca
     4981 ccgcgaaccg acgaggccat ccgctcccat ggagttccgc ggacacggaa aagttgtgtg
     5041 gcgagaacat tccctcgggg cgcaaggagt tcgatgtcgc gagttcgaca tcgaactcga
     5101 gaacatcagc tgtttcctca gtcacacaag tctgccaccg acgcgcaaaa cgcacgatcc
     5161 tgtcttctgg agcaaccgcc tcttcgcgct ggcgcaactc ctacacgact cgacttgcta
     5221 tcggctccct ctcgatcgcg taatcgaaat cggcctgcag gtgaaactct ggacgcgccg
     5281 gccccggccg ctcagaacca gccgagaacc gtgtcccgga tccaggccca gattccgccc
     5341 ttggcgacag gcttgagcgc gaccagcggc gcgcgggcga cggtcttgcc gtcggcgctc
     5401 acggtgagtt cgccgaccgt ctgcttcgcc gcgaccggcg ccttgacgtt ggcatcgacg
     5461 tgggtggaaa tcttcatgtc ggggaactcg ccgagcgcag ccgtcacgta gagcggcgcg
     5521 gcaagccccg cctccacgta tttgtcttcg cccttccaga cgcgaatctt ggcgagggcc
     5581 ttgccggcat cctccatctt gcgcgtctgg aagaaacgaa atccgtaatc caccagcgat
     5641 tcggtgacct tcgcgaggtg gcggtagttc gccatattgt cggtcttcga gaccccgggt
     5701 acgcccatca ctaccgcgat catccgccgg tttccgcgct ttaccgacgc gacgaggcaa
     5761 tagccggcgt ccgaggtgta gccggtcttg agcccgtcgg cgtcggaatc gagccacagc
     5821 agcttgttcc agttggtctg gtggatcttg ttccaggtga aatccttctg gccgaagtag
     5881 tggtagtact gcggatagtc gcgaatgatc cgccgcgcaa gccgcgcgag gtcgccggca
     5941 gaagtgtact gtccgtcgcc ggcgagtccg gccggatctt cgtagtgcgt attcgcgagt
     6001 ccgagttttt tcgcatcggc attcatcatg ttgacgaagc ccgcctcggt gcccgcaacc
     6061 ccttcggcga gcgccatggc cgcgtcgttc cccgagggaa tcagcatgcc ctggagaagt
     6121 tcgttgacgc tgacctgcga gccgagcttg atgaacatgc gcgagccgat ctcgcgccag
     6181 gcgttttcgc tcaccgtgaa gggcgtgtcg aggcggatgt gcccggcggc gaggtcctgg
     6241 aagacgacat aggcggtcat cagcttcgtc gtgctcgccg gcgcgcggtg cgcgtcggca
     6301 tcgcgggcgg cgagaatctg gcccgtgcgg taatccatca gcacgtagga cgccgcgctc
     6361 aagggcggcg gcggcggcac cggcacggcg gccagggcgg cgccccccgc ggcgaaaatc
     6421 ccgaaagcga cgatcaaacg aaacatgcgc cttccgaagc gaagcaggcg cacattgtag
     6481 cgagccgacg gcgcgggcgc gctatgctgt ccggcaggct cgtcggagcg cgtgcatgct
     6541 gagagacagc gaaagccgct ggggctcgct cagtcagttt ttccactgga gcgtcgcggc
     6601 tctcattttc gctctcatcg cgctcggctg gctcgtgcgc ctcacgccgc tctcccccgg
     6661 ccagatcacg ctcttctact ggcacaaatc gctcggaatg ctggcgctcg cgctcgttct
     6721 cgtccgtctc ggctggagag cggggaatcg cccgccgccg ctgcccggcg gactgccgcg
     6781 ctgggaggcg ccgctcgccc ggaccacaca tgtcgcgctc tatctcctca tcctcctcat
     6841 gcccgtttcc ggctggctcg tcaattcggc ctccggcatc ccattcaaga tcttctgggt
     6901 cctgccgctg ccggcgatcg ctcccgtatc ggcgggcctg gagcatgcct tcgaactcct
     6961 gcatctggcg agcttctcgg cgctggctct cctcctcgtc gggcacatcg gcgccgccct
     7021 gcggcaccac ttcctgctcc ataatgatgt tttcctgcgc atgctcccgc gcgggcgtcg
     7081 gaggacgcga tgatgcgaat ggcgctccca ttgctggcgg cgatcctgct cgccggaacg
     7141 gcgggcgccg ccgcgacggc tccgggctgg cattcggttg cggacgggag ccggatcggc
     7201 ttcgtcgcgc gctggcaggg aacgccggtg cccggcagtt tcgagcaatt ctcggtgcag
     7261 gcgcgcttcg atccgaatca tcccgcaggc ggcgagatca cggtcgacat cgcgagcgcg
     7321 agcgttcatg cgcaaagcgc cgacgtgacg cgagcgctgc gcgctcccgc gtggttcgac
     7381 gtgcagcatt atccgcaggc gagcttccgc tcgcagaaaa ttctcgcatc gggagcacac
     7441 tcggtccgcg tgagcggaac gctgcgcatc aagggacaga gccgacaggt cacctttccg
     7501 ctgaacttcg cgagcaaggg ctcgcgattg atcctcgacg gcagcctcga cctgcggcgc
     7561 agcgacttcg ggatcggcag cgggcagtgg gcgggcggca agatcatcgc cattcccgtc
     7621 accgtcgatt tccatgtggt gctggacccc gactgagtga ttcgccggtt cgtcctcgtc
     7681 gcgggtctgg gcacgatcct cggcgcctgc gccgctccga aaccgcccgc gccctccgcc
     7741 gcggcgccga agcccgcgat ctccgcggcg tatcgcgagg acgcgagggc aggccgagcc
     7801 gtctatcgcc tcgacgccgc ggcctcccgg gtcgagattc tcgtgggacg ggcgggtgca
     7861 ttcgcgagcg cgggacacct ccacgtcatc gaggtcggcc ggctcgaagg ctacgcgcgg
     7921 ctcgcgaatg gcggcggccg tgcggacctc gttttcccgc tcgccgcgat ggttgtggac
     7981 ccgccggccg cgcgctccgc cctcggcggc gcctacgctg agccgctcgg agaatcgcaa
     8041 cgcgaaggga cgcgcgcgaa catgctcggg gcgaagggac tcgacgccgc gcgctatccc
     8101 tgggcgcatc tcgcgatcac ggcccccgcg accgcgagcg ggagcgtgcc gatcacggca
     8161 gccatcagct tgcacggaat aacgcgccaa ttgtccgcga agactgccct cgccttcgat
     8221 gcgaagcatc tgcaagcgga cggcgagctg catctgcgcc agagcaattt cggaatcacg
     8281 ccctattcgg tgctgctcgg agcgctcaag gtcgccgaca cactcgacat ccgctaccat
     8341 ctgcggttca cgcgctggtg cccggttccc ggtccgactt catgttgaac cccgatctcg
     8401 atgttgcggc gcgggccgcg gaatttgcgc atcgccgccg cgttctggta ccacaggcgt
     8461 tcaccgcgga aaccgccgag aggctcgcgc gctgcctgcg cgaagaagtt ccctggggac
     8521 tcgcgacgcg cagcgggggc gagtcgcgct ggttgcgcac ggaggaactg cgcacgcttc
     8581 gagccaatcc ggcggaatgg cgcaagcttc tcgaagaggt tcacaacgag gcgcgctcgg
     8641 gctaccagtt tttttacaac agctaccaga tgatcgcggc ctacaaggaa cgcctcgatc
     8701 cgcagctgta tttgcatcgt ttcctcgagt ttctgaacag cccgccgatg atcgagttcg
     8761 ctcgccgcgt gaccggcatg gccgacattg cgaaggccga cgcgcaggcg acgcgctatc
     8821 ttcccggtca ctttttgcgc cgccacgacg acgtctaccg ggaaggcgaa cgcgacagcc
     8881 ggcgggccgc ctacgtcatc aatctcaccc gcggctggca ggccgactgg ggagggctgc
     8941 tgcaatttct cgacgcggcg cagaacgtgg aagaaacctg gacgccggac tacaacgtgc
     9001 tcgcgatctt cgccgtcccg acctggcact gcgtgtcctg cgttgcaccc ttcgcgaccg
     9061 aaccgcgcct cgcgatcacc ggctggttcc gcacgcactg aatcgtgacg cgtgcacagt
     9121 aaagtcggcc cctgcacggc ctgtgaagcg caaacgggat ttctgctttc cgcccgacag
     9181 cgtcgcaggc gcggctttgc cgcgaacgaa gtgagaggtt tcactctcta acggcgagcg
     9241 gcctgccaac gccgcgggcg gcgaaggggg agcgagacaa ggcatacaat gcagcctatg
     9301 gcccgcaata aagacacgaa gcggcttgag gcccggacac cctgcgccga gaccatcctc
     9361 gccgccgtgc acgagcaccc gatcatgcga aatttgtgga ttggtatgtt gtttgccggt
     9421 gtctccgcct gtgcgcaagc gccggctact gcgacgatta cgcccgcttc tgacgcatct
     9481 ccggcgcgcg gggccctgca aaacaaggcg cgcgcagccg tagcagcctc atccgtcagc
     9541 ttttacgtcg aggacggcga tctgccaccg tacgagttga tcaaacggtt atccgtttcc
     9601 ggcatcggcg gctgcaccaa tgcaaccaaa acgatgaaat tgctgatcga ggcgaaaacg
     9661 caggcggcgg cgctcggcgc caacggcgtg ctgttgcagt tgcacggtac gatgtcctcg
     9721 gggatgacca tcgtcaaagg aaaccggagc gttttttccg ttgcgcagta cgccggcagc
     9781 gcccccggtg gcagcacg
//
LOCUS       DASUJG010000205         2720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_184819, whole genome shotgun sequence.
ACCESSION   DASUJG010000205 DASUJG010000000
VERSION     DASUJG010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184819"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VFX38_04380"
     CDS             complement(<1..186)
                     /locus_tag="VFX38_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314132.1"
                     /translation="MADDPALLNAATANFAGLSRCPAREDARISIRLRWNDLATATVS
                     FAVSVKGSRMTLAGEGIL"
     gene            310..1689
                     /locus_tag="VFX38_04385"
     CDS             310..1689
                     /locus_tag="VFX38_04385"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5314133.1"
                     /translation="MILFAHSNFMVQDDKQMARMRPYAPLSTLIAAALLRREGHEVAL
                     FDATFAMGIDAFERRLRATRPSVVALMEDNFNFLTKMCTTVRRESALAMIRLASERGC
                     FVAVNGPDASDNPAAYFQAGARAVIEGDGEATMARVAGLWAKGKTDLREIAGLVLPET
                     GTTGPHRPTRELDALPLPAWDLVDVENYRRAWGGSLSWNVAASRGCPYACNWCAKPTF
                     GRRYTVRSAENVVAEMAAIKREIAPDHLWFTDDILGLDPDWIEAFADAIERHDARLPF
                     SMQSRVNLITPRVAAGLAKAGAAEVWLGVESGSQAILDAMDKGTQVNGARTATRLLRQ
                     HGIRVCWFIQLGYPSEEWDDILATRDLIRDEMPDDIGVSVAYPLPGTVFHDRVRAQLG
                     AQRNWHDTGELAMLFQGTYETAFYRRIRDLLHREVETGKADTFGWQVLDRQAHDYRSP
                     TPMCVVAGG"
     gene            1693..2499
                     /locus_tag="VFX38_04390"
     CDS             1693..2499
                     /locus_tag="VFX38_04390"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5314134.1"
                     /translation="MASADLPLPARAFDTIAASFDARFGQWESVAAQRRAVRRELAKA
                     FPPGANVLEIGGGTGEDALWLAERGRSVVMTDISPRMVQRARTKFAGRQRLRAQVAAA
                     EGLDRLDLGEAGFDGAFSNFAALNCVEALEPVARGLARLVKPSGAVIMVVFGTCCPGE
                     WIVEGLRDNPRAMFRRFRRTPVRARLGGNAFLVRYFRTAELVSAMAPWFDFAGRRGLG
                     VFVPPSAAEPWITRHPRLLSALERLDDRLSSSLAGLGDHVLHRFVRRADD"
     gene            2504..>2720
                     /locus_tag="VFX38_04395"
     CDS             2504..>2720
                     /locus_tag="VFX38_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314135.1"
                     /translation="MLDPKLTAFARAYAQQREAEGRRLSKAELLALPWLRHGPQAGQW
                     AIRARTFEAFMMRVLRPAARRQARPLTV"
BASE COUNT          411 a          910 c          936 g          463 t
ORIGIN      
        1 gagaatgcct tcgccggcga gcgtcatgcg tgaacctttg acggagaccg cgaaacttac
       61 cgtggcagtg gcgaggtcgt tccagcgcag gcgaatgctg atcctcgcat cctcgcgagc
      121 ggggcagcgt gacaggcctg cgaagttcgc ggtcgccgcg ttgaggaggg cgggatcgtc
      181 ggccatgatt gcagcaggaa ccccaagggg cgcaaacctc agcaccggtc ccggccgccc
      241 cggcactgcc tcactcgcct tcgctcgcac gccgatcatc tccgtttgct ctggccgggc
      301 ggttcgcccg tgattttgtt cgcgcactcc aacttcatgg ttcaagacga caagcaaatg
      361 gcccgcatgc ggccgtatgc tccgctgtcg acgctgatcg ccgccgcctt gctcaggcgc
      421 gaggggcacg aagtggcgct gttcgacgcc accttcgcga tgggcatcga tgccttcgaa
      481 cggaggcttc gcgcgacgcg cccgtcggtc gtcgcgctca tggaggacaa cttcaatttc
      541 ctgaccaaga tgtgcaccac cgtccgccgc gagagcgcgc tggcgatgat ccgcctggcg
      601 agcgagaggg gctgcttcgt cgcggtcaac gggccggatg cgagcgacaa tcccgcggcc
      661 tatttccagg caggcgcccg agccgtgatc gaaggcgacg gcgaggcgac gatggcgcgc
      721 gtcgccgggc tgtgggcgaa gggcaagacc gacctgcgcg agattgcggg cctggttctg
      781 cccgaaacgg gcaccaccgg gccccatcgg cccacgcggg aactcgacgc gttgccgttg
      841 ccggcctggg acctggtcga cgtcgaaaac taccggcggg cttggggcgg ctcactgtcg
      901 tggaacgtcg ccgcctcgcg cggctgcccc tacgcgtgca actggtgcgc caagccgacg
      961 ttcggccggc gctacaccgt gcgttccgcg gagaacgtcg tcgccgagat ggcggccatc
     1021 aagcgcgaaa tcgcgccgga ccatttgtgg tttaccgacg acatcctggg cctcgacccg
     1081 gattggatcg aggctttcgc cgatgcgata gagcggcatg acgcgcgcct gccattctcc
     1141 atgcagtcgc gggtcaacct gatcaccccg cgcgtcgctg ccggattggc gaaagccggc
     1201 gcggccgaag tgtggctcgg cgtcgaatcc ggttctcagg ccatcctcga cgcgatggac
     1261 aagggcacgc aggtgaatgg ggcgcgaacc gcgacccgcc tcctgcggca gcacggcatc
     1321 cgggtgtgct ggttcatcca gctcggctat ccctcggagg agtgggacga tatcctggcg
     1381 acacgagacc tgatccgcga cgagatgccg gacgatatcg gcgtctcggt cgcctacccg
     1441 ctgcccggca cggtattcca cgatcgggtt cgcgcgcagt tgggggcgca gcgcaactgg
     1501 cacgataccg gcgagctggc gatgctgttc cagggaacct acgagaccgc gttctatcgc
     1561 cgcattcgcg acttgttgca tcgcgaagtg gagacgggaa aggccgatac tttcggctgg
     1621 caggtcctcg accgacaggc tcatgattat cgctcgccca ctccgatgtg cgtcgtcgca
     1681 gggggctagc tcgtggcctc cgccgacctg ccgctgcccg cgcgcgcgtt cgataccata
     1741 gccgcgagtt tcgacgctcg cttcggacag tgggaaagcg ttgccgcgca gcggcgtgcc
     1801 gttcggcgcg agctggcgaa agccttcccg cccggcgcca acgttctcga aatcggcggc
     1861 ggcaccggcg aagacgcgct ttggctcgcc gagcggggcc gcagcgtggt gatgaccgat
     1921 atctcgccgc gcatggtcca gcgcgcgcgc acgaagttcg ccgggcggca acggctgcgt
     1981 gcgcaggtag ctgccgccga ggggctcgac cggcttgatc tcggtgaggc aggattcgac
     2041 ggggcttttt cgaacttcgc cgcgctcaac tgcgtcgagg cgctcgagcc tgtcgcgcgc
     2101 ggcctggctc gcctggtgaa gccgagcggg gcggtcatca tggtcgtatt cgggacgtgc
     2161 tgcccgggcg agtggatcgt cgaaggattg cgcgacaatc cgcgggcgat gtttcgccgc
     2221 tttcgccgca cgccggttcg cgcgcgcctc ggcggtaacg cgttcctcgt gcgctacttc
     2281 cggaccgccg aactggtttc ggcgatggcg ccgtggttcg acttcgccgg gcggcgcggc
     2341 ctaggagtgt tcgttccccc gagcgcggcg gagccgtgga tcactcgcca tccacgcctg
     2401 ctgtcggctc tcgagcgcct cgacgatcgg ctctcatcgt cgctggcggg cctgggcgat
     2461 cacgtgctgc accgcttcgt tcgccgcgcg gacgattgat acaatgctcg atcccaagct
     2521 gacagccttc gccagggcct atgcccaaca gcgagaagcg gaagggcgcc ggctgtcgaa
     2581 ggcggaactc ctcgccctgc cctggctgag acacggaccg caggccggcc agtgggcaat
     2641 ccgtgcgcgc accttcgagg cgttcatgat gcgcgtgctt cggccggcgg cgcgccggca
     2701 agcgcgtccg ctcaccgtcc
//
LOCUS       DASUJG010000206         3042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_185275, whole genome shotgun sequence.
ACCESSION   DASUJG010000206 DASUJG010000000
VERSION     DASUJG010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185275"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VFX38_04400"
     CDS             <1..480
                     /locus_tag="VFX38_04400"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEX5314136.1"
                     /translation="HDRLLRPLVYAYTDSGHPDHNETISFDWDNDRAHDIRGGKEKLI
                     PIAPGMLDRLTAQLEISRELAAGVPLTEPYLVVKGGEINAYHLRRDGQKKIDTPTGRF
                     DTTVVVREDPDSHSTTVFWLAPQYRWLPVKIEQREPGEATYSFVLTHLQWLKQDGKP"
     gene            complement(525..1283)
                     /locus_tag="VFX38_04405"
     CDS             complement(525..1283)
                     /locus_tag="VFX38_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314137.1"
                     /translation="MRGAMKQLIVFRSRLLMTQVKSKRRVARLKRAQRQGKSALHFLH
                     MGKTGGTAIKHALRPFPVTGDYVIYLHHHGFGLRDVPQGEGVILFLRDPISRFVSGFL
                     SRQRQGRPRYFTPWTPGERQAFRHFRSPNDLGMALSSPDAAEKSKAEAAMRSITHVGG
                     YAKWLGSEDYLASRLADMYFVGLQESLQEDFELLKVKLGEPRIPALPTDPVSAHRSDP
                     GSQSRLESAAIENLKQWYREDYGLLAQCKRIRER"
     gene            complement(1428..1994)
                     /gene="dcd"
                     /locus_tag="VFX38_04410"
     CDS             complement(1428..1994)
                     /gene="dcd"
                     /locus_tag="VFX38_04410"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEX5314138.1"
                     /translation="MSVKSDRWIRRMAAEQRMIEPFEPGQVRENGGGRIISYGTSSYG
                     YDVRCADEFKIFTNINSTIVDPKAFDAKSFVDFRGACCVIPPNSFALARTVEYFRIPR
                     NVLVICLGKSTYARCGVIVNVTPLEPEWEGHVTLEFSNTTPLPAKIYANEGVAQMVFF
                     ESDEVCETSYRDRRGKYQGQRGVTLPHA"
     gene            complement(2005..3039)
                     /gene="apbC"
                     /locus_tag="VFX38_04415"
     CDS             complement(2005..3039)
                     /gene="apbC"
                     /locus_tag="VFX38_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEX5314139.1"
                     /translation="MPEPWLGGRMGDADWPVAWEISAGAIRLEIELGYPLRRSRAATA
                     QTIRAALLSAADEIPLEIILHSEIRAHPVQPSLQPLAGIANVIAVASAKGGVGKSTVA
                     VNLALALADEGARVGMLDADIYGPSQPRMLGLSGHPRSDGKRLEPLAAFGLKVMSAGF
                     LVDPATPMIWRGPMVTQALTQLLANTDWGELDYLVVDMPPGTGDIQLTLAQRVPVAGA
                     VIVTTPQEIAVADARKGLAMFRKVEVPVLGIVENMTGFRCPHCGEVSEIFGAGGGARL
                     AEEAKVALLGRIPLDPRIQSAADGGAPSVAAEPEGDNARDYRAAALKMAAVLALRARA
                     VSAGPEISIE"
BASE COUNT          583 a          990 c          919 g          550 t
ORIGIN      
        1 cacgatcgcc tgttgcgtcc gctcgtttac gcctacaccg acagcggaca tccggatcac
       61 aatgaaacga tttccttcga ctgggacaac gatcgcgccc atgacattcg cggcggcaaa
      121 gaaaaactga tcccgatcgc ccccggcatg ctcgatcgcc tgacggcgca actggaaatc
      181 tcacgcgagc tcgccgccgg cgtgccgctc accgagcctt atcttgtcgt gaagggaggc
      241 gagatcaacg cctaccacct gcgccgtgac gggcagaaaa aaatcgacac tccgaccggg
      301 cgcttcgaca cgacggtcgt ggtgcgcgaa gaccccgact cgcatagtac gacggtgttc
      361 tggctcgcgc cgcagtatcg ctggctcccc gtcaagatcg agcagcgcga acccggcgaa
      421 gcaacctaca gcttcgtcct cacccatctc cagtggctga agcaggatgg caagccgtaa
      481 ccctccacac tattgcgatg gtgtccgtgc ccgaagatct ccgatcaacg ttcgcggatc
      541 ctcttgcatt gggccagaag cccatagtct tcccggtacc actgtttgag attctcgatt
      601 gccgcgcttt ccagccgact ttgcgacccg ggatcgctcc ggtgggctga aaccggatcg
      661 gtcggaagcg cgggaattcg aggctcgccc aatttcactt tgagcagttc gaaatcctct
      721 tgcaggcttt cctgcaatcc gacgaaatac atgtcggcca accgggacgc caggtagtcc
      781 tcgctgccga gccattttgc atagccgcct acatgcgtga tactgcgcat ggcagcctcc
      841 gccttcgact tttcggcggc gtccggagag gacaatgcca tgcccagatc gttgggactg
      901 cggaaatggc gaaacgcctg ccgttccccc ggcgtccacg gggtgaagta gcgcggtcgt
      961 ccctggcgct gacgactgag aaatccactg acgaacctgc tgatcggatc gcgcaaaaac
     1021 aggatgactc cctccccttg cggaacgtcc cgcagaccga aaccatggtg gtgaaggtag
     1081 atcacgtaat cgcccgttac cggaaacggc ctcaaagcat gtttgatcgc ggtcccgccc
     1141 gttttcccca tatgaagaaa atgcagggcg ctcttgcctt gtcgttgcgc acgcttcaat
     1201 cgcgccaccc gtcgcttgct cttgacctgc gtcatcagca atcgggatcg gaatacgatc
     1261 aattgtttca tcgcgccgcg cacacaaaat gagggttgga aaaggcgagc gagtttaagg
     1321 cccggcgaac cacaacgtca agcccaatgt ttcaggcgtg ccatgagagc gcgctcagga
     1381 tgccacacgc aagtgacgaa gcggagcggg ctcacgacac gtcgtcttca ggcatgcggc
     1441 agggtaacgc cgcgctgtcc ctgatacttg ccgcgacgat cgcggtagct cgtttcgcag
     1501 acttcgtcgg attcgaagaa caccatctgc gcgacgcctt cgttcgcata gatttttgcc
     1561 ggcaacggcg tggtgttgga aaactcgagc gtaacgtgac cctcccactc gggttccagc
     1621 ggcgtcacat tcacgatcac gccgcagcgg gcataggtac tcttccccag gcagatcacg
     1681 aggacgttgc gcgggatccg aaaatactcg acggtgcgcg cgagagcgaa ggagttgggc
     1741 ggaatcacgc agcacgcgcc ccggaaatcc acgaagctct tcgcatcgaa ggccttgggg
     1801 tccacaatgg tgctgttgat gttggtgaaa atcttgaact cgtcggcgca gcgcacgtcg
     1861 tagccgtaac tcgaagtgcc gtaggaaatg atgcgcccgc cgccgttttc gcgaacctgt
     1921 ccgggttcga acggttcgat cattcgctgt tccgcggcca tgcgccggat ccaccggtcc
     1981 gatttcacgc tcatcgctgg ttccttattc gatgctgatt tcaggcccgg ccgacacggc
     2041 gcgagcacgc agcgccagca cggccgccat tttaagcgcc gccgcgcggt agtcgcgggc
     2101 attgtcgcct tcgggctcgg cggcaacgct cggcgcgccg ccgtcggcgg ccgactgaat
     2161 gcgaggatcg agcgggatcc gcccgagaag cgccaccttt gcttcttcgg ccagacgcgc
     2221 gccgccgcct gcgccgaaaa tctcgctgac ttcgccacaa tgcgggcagc ggaaacccgt
     2281 catgttctcg acgatgccga gcacagggac ctcgaccttg cggaacatcg cgagtccctt
     2341 gcgggcatcg gcgacggcga tttcctgcgg cgtcgtcacg atcaccgcac ccgcaaccgg
     2401 cacccgctga gcgagagtca actgaatgtc acccgtgccc ggcggcatgt ccacgacgag
     2461 atagtcgagc tctccccagt cggtgttggc gaggagctgc gtgagcgcct gcgtcaccat
     2521 cggaccgcgc cagatcatcg gggtcgccgg gtccacgaga aagcccgcgg acatgacctt
     2581 gagcccgaag gccgcgagtg gttcgagccg cttgccgtcg cttcgaggat gcccggagag
     2641 cccgagcatg cgcggctggc tcggtccgta gatgtcggcg tcgagcatgc cgacacgcgc
     2701 gccctcgtcg gcgagcgcga gcgcgagatt gacggcaacg gtggatttgc ccacaccgcc
     2761 cttggccgaa gcgacggcga tcacgttcgc gatgccggcg agcggctgca ggctcggctg
     2821 taccggatgc gcccgtatct ccgaatgcag gatgatctcc agcgggattt cgtcagccgc
     2881 agaaagcagc gccgcgcgga tcgtctgcgc cgtggcggca cgcgagcggc gcagcgggta
     2941 gccgagttcg atttcgaggc ggatcgcccc tgccgaaatt tcccaggcga ccggccagtc
     3001 cgcatcgccc atgcgcccgc cgagccaggg ctccggcatg gc
//
LOCUS       DASUJG010000207         5985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_795459, whole genome shotgun sequence.
ACCESSION   DASUJG010000207 DASUJG010000000
VERSION     DASUJG010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795459"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VFX38_04420"
     CDS             <1..389
                     /locus_tag="VFX38_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014003529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX5314140.1"
                     /translation="AAVRAQAAGRVFVVFGCGGDRDRGKRSQMGAVAAGAADRLVLTN
                     DNPRGEDPAAIVAEIRTGIPAGTEVAVIPDRSQAIAAALGEARAGDVVLVAGKGHEAV
                     QVIGHERRRYSDRQTVERLLLGSLSW"
     gene            383..1735
                     /gene="murF"
                     /locus_tag="VFX38_04425"
     CDS             383..1735
                     /gene="murF"
                     /locus_tag="VFX38_04425"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX5314141.1"
                     /translation="MVMPRTFAALAQATGGRLAGSDGEFGTVISDSRRMEAGALFVCL
                     RGPHFDGHEFAEAAAAAGAAGFLASRELAPALPCVEVEDSAAALARFAQDWRAAQAAR
                     VVAITGSNGKTTTKDLLAAILAQVAPTLATIGNLNNLIGVPLTLARLGEAHRYAVVEL
                     GTNAPGEIARLGEIARPDAAIVTSIGPAHLEGFGTVDGVAREKGSLFAALAPDGLAVV
                     PAGSPWLESWRAASVVRRWVGFGLDADAEVTAHSIEAGAAGMRFTLVTPDGSAPVSLR
                     LLGRHNVSNALAAAAAAWGLGVPPDSIGRGLALAGPAAGRLVPRRLASGVLLLDDSYN
                     ANPASVTAAVATAAALEPEVWLVLGDLAELGSGAAAWHRALAAEVRAAGATRLFALGP
                     LAAEAADAFGADGESFVEVEALAAAVAPRLVSGVAVVVKGSRSARMERVVAALAAEET
                     "
     gene            1736..2818
                     /gene="mraY"
                     /locus_tag="VFX38_04430"
     CDS             1736..2818
                     /gene="mraY"
                     /locus_tag="VFX38_04430"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008045924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX5314142.1"
                     /translation="MLLWLADYLSQFLAGFRVVDYITLRTVLAVLTALVLSFAFGPLF
                     IRKLALHKVGQFVRHEGPVTHLGKSGTPTMGGVLVIAAIIVSTLLWGRLDNRFVEVAL
                     VALAVFGAIGAVDDLIKLRGARSLGFTARRKFLLQSLAAVGLGVWLYLTVQSPAETEF
                     FVPFAKTIVIPMGGWFLLSCYLVVVGSSNAVNLTDGLDGLAIMPTVLIMGALGVFAYT
                     TGNIKIAEYLGIPYVAGVGEVAVFCGALVGAGVGFLWFNTYPAQVFMGDIGALALGAA
                     IGIVAIMVRQEIVLFIMGGVFVIETLSVIVQVGSFRLTGRRVFSMAPLHHHFELKGWA
                     EPKVIVRFWIVTVILVLIGLSTLKVR"
     gene            2818..4212
                     /gene="murD"
                     /locus_tag="VFX38_04435"
     CDS             2818..4212
                     /gene="murD"
                     /locus_tag="VFX38_04435"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX5314143.1"
                     /translation="MKMAIADWGSGSGGSAVRNSGFVGRRCLVLGLGKTGVSVLRYLQ
                     GAGARVRATDRDSGRAAAAGDGWCTPEEALAALAETDLLVVSPGVPSDSALLAKARER
                     DTEIVGDIELFARALSSTPHPRLPAPVVAVTGTNGKSTVAMLVAAMAERAGRRVAAGA
                     NLGTPALELLDPAVELYVLELSSFQLEPTKSLAPLAAAVLNVSPDHLDRYPDLASYAA
                     AKARIFRHCEVAVFNRADPLVRQMIAKHRRCVGFGLDEPAGEDYGLRLREGEPWLCRG
                     RQWLLAAAEVPLAGSHNLANVLAAWALAAAAGVPDEAIAPAVREFRGLPHRLSPVGLR
                     RGVHYLDDSKATNVSAASAAIAGLDGPLVVIAGGQGKGQDFGDFADLLVLRARAVILL
                     GVDAPRIASAIAGRIPVIRADSMSAAVAAAARCAQPGDRVLLSPACASLDQFRDYRER
                     GEAFAAAVEALDEH"
     gene            4202..5365
                     /gene="ftsW"
                     /locus_tag="VFX38_04440"
     CDS             4202..5365
                     /gene="ftsW"
                     /locus_tag="VFX38_04440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEX5314144.1"
                     /translation="MSTEALLSGRVAARHMPQVPDLPLCALAFALLGIGLVMVASATL
                     QTTTGADGLSDFLRQLVFAFVGIGVAVLLYTLPLDYLERLRYPALALALALLVAVFVP
                     GLGHSINGSERWLGIGAFTVQASEPARVLILVYIAGYALAHREGLASSFWNLVKPIAV
                     VAFAAVLLLAEPDFGGTIVLFVTVLGMLFFAGARKRDALILLLVFGGTALALALSSSY
                     RLERLTTFLNPWAVRQGSGFQLTQALLAAGHGGVFGTGLGESVEKLLYLPEAQSDFIF
                     SVLAEELGLFGVATVVVLYLALTLRALAIARRAAACGLGFAGNLAFGLGLLLGVQAFV
                     NIGVNLGVLPTKGLTLPLMSAGGSSLIVDLAVCGLLLRVARETRIVRTRGALR"
     gene            5362..>5985
                     /locus_tag="VFX38_04445"
     CDS             5362..>5985
                     /locus_tag="VFX38_04445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5314145.1"
                     /translation="MSTRPILILAGGTGGHVIPALAVAEVFRRRSVPVHWLGGATGIE
                     ARMVSAAGIPFTALPGRGLRGTGLGRKLLGPPRLIGAVARAWRLIRRLRPRAALGFGG
                     YASGAGGIAAWLARCPLVVHEQNAIAGYTNRALGRFAARLLTGFPDTRGLAGEFVGNP
                     VRGELAGLAPPAERYGGRQGPLHLLVIGGSQGAQILNESVPAALARCQ"
BASE COUNT          744 a         2094 c         2035 g         1112 t
ORIGIN      
        1 tggccgcggt gcgcgcgcag gccgcgggac gcgtgttcgt ggtgttcggc tgcggcggcg
       61 atcgggatcg cggcaagcgc tcgcaaatgg gcgccgttgc cgcgggcgcc gccgatcggc
      121 ttgtgctcac caacgacaac ccccgcggcg aagatccggc ggcaattgtc gcggagattc
      181 gcacgggcat tcctgcgggt accgaagtcg ccgtcattcc cgatcgcagc caagcgattg
      241 ccgccgccct cggcgaagct cgcgcgggcg atgtcgtgct cgtcgccggc aagggtcacg
      301 aggcggtgca ggtgatcggc cacgagcgcc gccgttacag cgatcggcag acggtcgagc
      361 gtctgctcct cgggagtttg tcatggtgat gccgcggacc ttcgccgcat tggcacaggc
      421 gaccggcgga cgcctcgcgg gttccgacgg cgagttcggc acggtgatct ccgacagccg
      481 gcggatggag gcgggcgcgc tcttcgtctg tctgcgcggc ccgcatttcg acggtcacga
      541 attcgcggag gctgccgccg ccgcgggcgc cgccggattt ctcgccagcc gcgagctggc
      601 gccggcgctt ccctgcgtcg aggtcgagga cagcgcggca gccctcgctc gcttcgcgca
      661 ggactggcgc gcggcgcagg ccgcgcgggt cgtcgccatc accggctcga acggcaagac
      721 cacgaccaag gatctcttgg ccgcgatcct tgcccaggtc gctccgacgc tcgccaccat
      781 cggcaatctc aacaacctga tcggagtgcc gctcacgctc gcgcgactcg gcgaagcgca
      841 ccgctatgcc gtggtggaac tcggtaccaa cgcgccgggc gagattgccc gcctcgggga
      901 gatcgcacgg ccggacgccg ccatagtgac gagcatcggc cccgctcatc tcgaaggttt
      961 cggcaccgtc gacggggtcg cgcgcgagaa aggctcgctg tttgcggcgc ttgcgcccga
     1021 cggcctcgcc gtcgtgcccg cgggctcgcc ctggctcgag agctggcggg cggcgagcgt
     1081 cgtgcgtcgc tgggttggct tcggtctcga cgccgatgcc gaagttaccg cgcattcgat
     1141 cgaggccggc gcggccggga tgcgctttac cctggtcacg ccggacggca gcgcgccggt
     1201 ttctctgcgc cttctcgggc gacacaacgt gagcaacgcg ctcgccgccg ccgccgccgc
     1261 ctggggcctc ggggttccgc ccgacagcat cggccgcggc ctggcgctcg ccggaccggc
     1321 cgccgggcgg ctcgtcccgc gccgtctcgc ctcgggcgtg ctgcttctgg acgatagcta
     1381 caacgccaat ccggcctccg tcaccgccgc ggtggcgacg gcggcggccc tggaacccga
     1441 ggtctggctg gtgctggggg atctcgcgga actcggttcc ggcgctgccg cctggcaccg
     1501 cgcgctcgca gcggaggtac gagccgccgg cgccacccgc ctttttgcgc tcggcccgct
     1561 tgccgccgag gcggcggatg ccttcggcgc ggacggcgag agcttcgtcg aggtggaggc
     1621 gctcgccgcg gccgtcgcgc cgcgactcgt ctcgggcgtg gccgtcgtcg tgaagggctc
     1681 gcgcagcgcc cgcatggagc gtgtcgtcgc cgcgctcgcg gcggaggaga cctgaatgct
     1741 cctgtggctc gccgactatc tcagtcagtt cctcgcgggt ttccgcgtgg tggactacat
     1801 caccctgcgc acggtgctcg cggtactcac tgcgctcgtt ctctccttcg ccttcggccc
     1861 gcttttcatt cgcaagctcg cgctccacaa ggtcggacag ttcgtgcgtc acgagggccc
     1921 ggtcacgcac ctcggcaaga gcggcactcc gaccatgggc ggcgtgctcg tgatcgcggc
     1981 gatcatcgtc tcgacgctgc tctggggccg gctcgacaac cgattcgtgg aggtcgccct
     2041 cgtcgcgctc gcggtcttcg gcgcgatcgg cgcggtggac gacctgatca agttgcgcgg
     2101 cgcgcgttcg ctcggcttca ccgcgcggcg caagtttttg ctgcagtcgc tcgccgccgt
     2161 cggtctcggg gtgtggctct atctcacggt gcaaagtccg gccgaaaccg agttcttcgt
     2221 tcccttcgcg aagacgatcg tgatccccat gggcggctgg tttctcctct cctgctatct
     2281 cgtggtcgta ggcagttcca atgccgtcaa tctcaccgac ggcctcgatg gcctcgcgat
     2341 catgccgacc gtgctcatca tgggggccct cggcgtcttc gcctatacca ccggcaacat
     2401 caagatcgcg gagtatctcg ggattcccta cgtcgcgggc gtcggggagg tcgccgtctt
     2461 ctgcggcgcg ctggtcggag ccggcgtcgg cttcctctgg ttcaacacct atcccgcgca
     2521 ggtcttcatg ggcgacatcg gagcgctcgc gctcggagcc gccatcggca tcgtcgcgat
     2581 catggtgcgg caggaaatcg tgctcttcat catgggcggg gttttcgtga tcgaaaccct
     2641 gtcggtgatc gtgcaagtcg gatcgttccg cctcaccggg cggcgcgtct tcagcatggc
     2701 gccgcttcat catcacttcg aactcaaggg ctgggcggaa cccaaggtca tcgtccgctt
     2761 ctggatcgtc accgtgattc tggttctgat cggcctctcg acgctcaagg tgcgctgatg
     2821 aagatggcga tcgcggactg gggatcgggg agcggcggga gcgcggttcg taactccggg
     2881 tttgtcggcc ggcggtgcct cgtcctcgga ctcgggaaga ccggcgtttc cgtgctgcgc
     2941 tatctgcaag gcgcgggcgc gcgcgtccgc gcgaccgatc gtgactccgg tcgcgccgcg
     3001 gccgccggcg acggctggtg tacgccggaa gaggcgttgg ccgcgcttgc ggaaaccgat
     3061 ctcctcgtcg tgtcgccggg cgtcccttcc gattcggcgc ttcttgcgaa ggcccgcgaa
     3121 cgcgacaccg aaatcgtggg cgacatcgaa ctgttcgccc gcgctctctc ttcgactccc
     3181 catcctcggc tcccggcccc cgtcgtggcc gtcaccggca ccaacggcaa aagcacggtg
     3241 gcgatgctcg tcgcggccat ggccgagcgc gccgggcggc gcgtcgcggc cggcgccaat
     3301 ctcggaaccc cggcactcga gttgctcgat ccggcggtgg agctctacgt cctcgagctg
     3361 tcgagctttc agctcgagcc caccaaatcg ctggcgccgc tcgccgcggc cgtgctcaac
     3421 gtcagtcccg atcatctcga ccgctacccc gatctcgcgt cctacgccgc ggccaaggcg
     3481 cgcatcttcc gccactgcga agtcgccgtg ttcaatcgtg ccgatccgct ggtgcgccag
     3541 atgattgcga aacatcgcag atgcgttggc ttcggactcg acgaacccgc aggcgaggat
     3601 tacggtttgc gtttgcgcga aggcgagccg tggctctgcc gcggccggca atggcttctt
     3661 gccgcggccg aggtgccgct ggcgggaagt cacaatctcg cgaacgtgct cgcggcctgg
     3721 gcgcttgcgg ccgccgccgg cgttcccgac gaagcgatcg cgccggcggt acgcgagttt
     3781 cgcggccttc ctcatcggct gagcccggtt gggttgcgac gcggagtgca ctacctcgac
     3841 gactccaagg ccaccaacgt gagcgcggcg agcgccgcga tcgccggcct cgacggtcct
     3901 ctggtcgtga ttgcgggcgg gcaggggaag ggacaggact tcggcgactt cgccgatctt
     3961 ctggtcttgc gtgcgcgcgc cgtgatcctg ctcggcgtcg atgctccgcg tattgcctcg
     4021 gcaattgccg gtcgcatccc ggtgatccgc gcagattcga tgagcgcggc cgtggccgcg
     4081 gcggcgcgct gcgcgcaacc gggcgatcgg gtgctcctct cacccgcctg cgcgagcctc
     4141 gatcagttcc gcgactaccg tgaacgcggc gaagccttcg ccgccgccgt cgaggctctc
     4201 gatgagcact gaagcgctgc tctcggggcg cgtcgcggcg cggcacatgc cgcaagttcc
     4261 ggacttgcct ctgtgcgcgc tcgcgtttgc gctcctcggc atcggtctgg tgatggtggc
     4321 atccgcgacg ctccagacga cgacaggagc tgacggtctg agcgatttcc tgcgccagct
     4381 cgtatttgcg ttcgtgggaa ttggcgtcgc ggtgcttctc tacaccttgc cgctcgacta
     4441 tctcgaacgg ctgcgctatc cggcgctcgc gctcgcgctc gcgctgcttg tcgccgtctt
     4501 cgtcccggga ctcggacatt ccatcaacgg cagcgagcgc tggctcggga tcggcgcctt
     4561 cacggtgcag gcctcggagc ccgcgcgggt gttgatcctc gtgtacatcg cgggctacgc
     4621 gctcgcgcac cgcgagggcc tcgcgagttc cttctggaat ctcgtcaaac cgatcgcagt
     4681 cgtcgcgttc gccgcggtac tgcttctcgc cgagcccgat ttcggcggca cgatcgtgct
     4741 cttcgttacc gtgctcggaa tgctcttttt cgccggggcg cgcaagcgcg acgcgctgat
     4801 cctgctcctc gtcttcggcg gtacggcgct cgccctcgcg ctcagttcct cctatcgcct
     4861 ggaacgtctc acgacctttc tcaatccctg ggcggttcgg cagggcagcg gatttcaatt
     4921 gacgcaggcg ctcctcgccg ccggtcatgg cggagtattc ggcaccggcc tcggcgagag
     4981 cgtcgagaaa cttctctacc ttccggaagc gcaatcggat ttcattttct cggtgctcgc
     5041 cgaagaactc ggcctcttcg gtgtcgccac ggtcgtcgtg ctctatctcg cgctcaccct
     5101 gcgtgcgctc gcgattgccc gacgcgccgc cgcctgcggt ctcggcttcg ccggcaatct
     5161 cgccttcggt cttggacttc tgctcggcgt gcaggcgttc gtgaacatcg gagtcaacct
     5221 cggagtgctg cccaccaagg gcctcaccct gccgctgatg agcgccggcg gttcgagcct
     5281 catcgtggac cttgcggtct gcggattgtt gttgcgggtt gcgcgggaaa cacggatcgt
     5341 ccgcacccgc ggagcgctgc gatgagcacg cggccgattc tcattctcgc cggcgggacc
     5401 ggcggtcacg tcattccggc gctcgccgtc gccgaagtgt ttcgcaggcg ttccgtgccg
     5461 gtccattggc tcggcggcgc gacggggatc gaagcccgca tggtgagcgc cgcgggcatt
     5521 cctttcacgg cgctgcccgg gcgcgggctg cgcggcaccg gtctcgggcg caagctcctc
     5581 ggtccgccgc gtctcatcgg ggccgtcgcc cgggcctggc gactcatccg gcggctgcgt
     5641 ccccgagccg cgctcggctt cggcggctat gcgagcggag cgggaggcat tgcggcctgg
     5701 cttgcgcgct gtcctctcgt ggtccacgaa caaaatgcga tcgcgggtta caccaatcgt
     5761 gcgctcgggc gcttcgccgc ccgcctgctc accggctttc cggatacccg cggcctcgcc
     5821 ggcgagtttg tcggcaatcc ggtgcgcggc gaactcgccg gacttgcccc gccggccgag
     5881 cgttacggcg ggcggcaagg tccgctgcat ctgctcgtga tcggcggaag ccagggtgcc
     5941 cagatcctca acgagagcgt gccggcggcg cttgcgcgct gccaa
//
LOCUS       DASUJG010000208         3670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_920296, whole genome shotgun sequence.
ACCESSION   DASUJG010000208 DASUJG010000000
VERSION     DASUJG010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920296"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VFX38_04450"
     CDS             <1..460
                     /locus_tag="VFX38_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEX5314146.1"
                     /translation="VTSDAWRAQTADRGERYLQGYLAIGEHSVVRVRIAGERRAWIGV
                     KEHRIGRARGEYEYPIPVGDARELLQLVTGALVEKNRYRVPHEGRLWELDEFLGANAG
                     LVVAEIELSAADEEFGRPSWLGPEVTAERAYYNAALALRPWNEWGREGGS"
     gene            478..939
                     /locus_tag="VFX38_04455"
     CDS             478..939
                     /locus_tag="VFX38_04455"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX5314147.1"
                     /translation="MSLADQHLVLEKDGRRFDYVVSTSHYGAGEERGSFRTPRGLHVI
                     RAKIGAGAPKGAVFVARRPTGEILTPALYAGASSRDWILTRILWLSGREPGRNRFGSV
                     DTMRRYIYIHGSPEERPVGTPSSHGCIRMRNDDVIELFDLVAVGTPVLIEE"
     gene            942..1985
                     /gene="nagZ"
                     /locus_tag="VFX38_04460"
     CDS             942..1985
                     /gene="nagZ"
                     /locus_tag="VFX38_04460"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEX5314148.1"
                     /translation="MLGPLIVDVAGTSLDAEDRELLAHPLVGGVILFARNCRKAAEVA
                     RLAEEVHALRKPPLLVTIDQEGGRVQRLTDDVTRLPPASLFGALWDEDPSSARALSRD
                     TGRLMAAELLALGVDLSFAPVADLGGRSRVIGDRAFHRDPQAVSDLAFAWMQGMHEAG
                     MRAVAKHFPGHGGVTGDTHVERPVDRRRHADLALADLLPFEHLIVNGLDAVMMSHVVY
                     PEVAPEPASFSPRWVTEILREGFDFQGAILADDLAMEGAASGGSVFERLDATLAAGCD
                     LAPLCNDRGAVKTLIANAGSIAPAPASGLRRARLLPTKRWDEAMLRADPRLAATREAL
                     ARLSGGDSFRLES"
     gene            1987..2574
                     /locus_tag="VFX38_04465"
     CDS             1987..2574
                     /locus_tag="VFX38_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX5314149.1"
                     /translation="MSSPSTYAPPGEMLHSAEAVQAAIAHQAEWLRPRLANENPLVVV
                     LLQGALYYAAWLTLALAIPLEIACVDVGRYGRGRSGGVLAWRQKLAADVGGRSVLLLD
                     DIFDEGATLAAVRAACIAAKAREVRSAVLLRKRHDRARAQEPDSAALEVPDCFVVGCG
                     LDDAGRWRNLPAIYALDERALPRSLPDRVAERIGT"
     gene            2628..3371
                     /locus_tag="VFX38_04470"
     CDS             2628..3371
                     /locus_tag="VFX38_04470"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HEX5314150.1"
                     /translation="MTDIAIIGGTGITRLPQLEVSRREVASTPYGEPSSPLTFGLIAG
                     CEAVFLARHGSGHRVSPHRVNYRANLWALKSVGVRRVIAIASVGGIRADLEAGRLAIP
                     DQIVDYTSGREHTYFDEEQVVHIAFTEPYDAGLRSLLVAAGRAAGLDVADGGVYGATQ
                     GPRLETAAEIDRMERDGCDMVGMTGMPEAALARELDLAYAACARVTNRAAGRGTMDIH
                     TEILVQMEQSLGAVYALLAALLPMMSASD"
     gene            complement(3368..>3670)
                     /locus_tag="VFX38_04475"
     CDS             complement(3368..>3670)
                     /locus_tag="VFX38_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 family protein"
                     /protein_id="HEX5314151.1"
                     /translation="VAGPSLAARIKPIVTKATASVGVTEKYKEFMASSEGGELGGALS
                     LLGGGKSGSNSLDLDDYVTKETINGLFYEIGKEEKAIRKDPAARTTDLLKEVFGGG"
BASE COUNT          559 a         1208 c         1237 g          666 t
ORIGIN      
        1 ggtcacgagc gatgcgtggc gtgctcagac cgccgaccgc ggcgagcgtt acctgcaggg
       61 ctatctcgcg atcggcgaac actcggtcgt gcgcgtgcgg atcgccggcg agcgccgcgc
      121 ctggatcggc gtgaaggaac atcggatcgg acgcgcgcgc ggcgagtatg aatatccgat
      181 tcccgtcggg gatgcgcgcg aactcctgca attggtgacc ggtgcactcg tcgaaaagaa
      241 tcgctatcgc gtgccgcacg aaggccggct ctgggaactc gacgagtttc tcggcgcgaa
      301 cgcgggactc gtcgtcgcgg agatcgaact ctccgccgcc gacgaagaat tcgggcgacc
      361 ttcctggctt ggaccggagg tgacggccga gcgcgcctac tacaacgcgg cgcttgcctt
      421 gagaccgtgg aacgagtggg gcagggaggg cggttcttga gtgcggcgga aattcgcatt
      481 tccctcgccg atcagcatct cgtgctggag aaggatggcc gccgtttcga ctatgtggtt
      541 tccacttcgc attacggcgc gggcgaggag cggggaagtt tccgcacgcc ccgcggactc
      601 cacgtcattc gcgcgaagat cggcgccggc gccccaaagg gcgcggtgtt cgtcgcccgc
      661 cgtccgaccg gcgaaatcct gacgccggcg ctctacgcgg gcgcgtcgtc gcgcgactgg
      721 attctcacgc gtatcctgtg gctttcgggc cgggaaccgg gccgcaaccg tttcggctcg
      781 gtggacacga tgcgccgtta catatacatt catggcagcc cggaggaacg gccggtgggc
      841 acgccttcca gccacggatg catccggatg cgcaacgacg acgtgatcga gctgttcgat
      901 ctcgtggccg tcggcacgcc ggtcctgatc gaggagtaaa ggtgttaggc ccgttgattg
      961 tggatgtcgc cggcacgagc ctcgacgccg aggatcgcga attgctcgcg catccgctcg
     1021 tcggcggcgt gatcctgttc gcgcgcaact gtcgaaaggc cgccgaggtt gcgcgcctcg
     1081 cggaagaagt tcatgcgctg cgcaagccgc cgctgctcgt gacgatcgat caggagggcg
     1141 ggcgcgtgca gcgtctcacc gatgacgtca cgcggcttcc tccggcttcg cttttcggcg
     1201 cgctctggga cgaagatcca tcttccgcgc gcgcgctttc ccgcgacacc ggccgtctca
     1261 tggcggccga actgcttgca ctcggcgtgg atctcagctt cgcgccggtg gccgatctcg
     1321 gcgggcgctc gcgcgtcatc ggcgatcgcg cctttcatcg cgatccgcag gcggtgtccg
     1381 atcttgcctt cgcgtggatg cagggcatgc acgaggcggg aatgcgtgcc gtcgccaaac
     1441 attttccggg tcatggcggc gtcaccggcg acacgcatgt cgagcgtccg gtggaccggc
     1501 gccgccacgc cgatctcgcg ctcgccgatc tgcttccctt cgagcatctg atcgtgaacg
     1561 gtctcgatgc ggtgatgatg agccatgtcg tctatcccga agttgctccg gaacccgcga
     1621 gtttctcgcc gcgctgggtg acggaaatac ttcgcgaagg attcgatttt cagggtgcca
     1681 tcctcgccga cgatctcgcg atggagggcg ccgcctcggg cggttcggtg ttcgaacgcc
     1741 tcgacgcgac gctcgccgcc ggctgcgatc tcgccccgct ctgcaacgat cgcggcgccg
     1801 taaagacctt gatcgcgaac gccggctcca tcgcgcccgc gccggcatcg ggcctgcggc
     1861 gcgcgcgcct gctgccgacc aaacgctggg acgaggcgat gcttcgcgcc gatccccggc
     1921 ttgccgcgac tcgcgaagca ctcgcgcgcc tttccggcgg cgactctttt cggctggaat
     1981 cctgagatgt cctcgccttc gacttatgcg ccgcccggag aaatgctgca ttcggccgaa
     2041 gcggtgcagg cggcgatcgc gcatcaggcg gaatggcttc gcccgcggct cgcgaacgaa
     2101 aatccgctcg tcgtggtgct gcttcagggc gcgctctact atgccgcctg gttgacgctc
     2161 gcactcgcga ttccgctcga aattgcctgc gtcgacgtcg ggcgttacgg ccgcgggcga
     2221 agcggtggcg tgcttgcctg gcggcagaaa cttgcggcag acgtcggcgg gcggagcgtt
     2281 ctccttctcg acgacatctt cgacgaaggt gcgacgctcg ccgcggtgcg cgcggcttgc
     2341 atcgccgcga aggcgcgcga ggttcgcagc gccgtgctgc tgcgcaaacg tcacgatcgc
     2401 gcccgcgcgc aagagccgga ttcggccgcg ctcgaagttc ccgactgctt cgtcgtcggc
     2461 tgcggcttgg acgatgcagg gcgctggcgc aatctgccgg ccatttacgc gctcgacgag
     2521 agagcgttgc cgcgttcgct cccggatcga gtcgcggaaa ggatcggaac gtagcgaagt
     2581 cggctggcaa gcgacagcga aaccgggata ccagaaggag agtttgcatg acggacatcg
     2641 cgatcatcgg cggcaccggc atcacgcgcc tgccgcaact cgaagtctcg cgccgcgagg
     2701 tggcctcgac tccctacggc gaaccttcga gtccgctcac cttcgggctc atcgccggat
     2761 gcgaggccgt atttctcgcg cgtcacggct ccggtcaccg cgtttctccg catcgggtga
     2821 attaccgcgc caatctctgg gcgctcaaga gcgtcggcgt gcgccgcgtc atcgcgattg
     2881 cctcggtcgg cggcattcgc gccgatctcg aagccgggcg cctcgcgatt cccgatcaga
     2941 tcgtggacta caccagcggg cgcgagcaca cctatttcga cgaggaacag gtcgtccaca
     3001 tcgcctttac cgaaccctac gatgccggcc tgcggagcct tcttgtcgcg gccggacgcg
     3061 cagccgggct cgatgtcgcg gatggcggcg tgtacggagc aacccaggga ccgcgcctcg
     3121 aaacggccgc ggagatcgat cgcatggaac gcgacggctg cgacatggtc ggcatgaccg
     3181 gcatgcccga ggcggcgctc gcacgcgaat tggacctcgc ctacgcggcc tgcgcgcgcg
     3241 tgaccaatcg cgcggcgggg cggggcacga tggacattca caccgagatt ctcgtgcaga
     3301 tggagcagag tctgggagcc gtgtacgcgc ttctcgcggc gctgcttccg atgatgtccg
     3361 cgagcgatta gcctccgccg aagacttcct tcagcagatc ggtggtgcgc gcggcgggat
     3421 ccttgcggat cgccttttcc tccttgccga tctcgtagaa gaggccgttg atcgtctcct
     3481 tcgtgacgta gtcgtcgaga tcgagcgagt tgcttccgct cttgccgccg ccgagaagcg
     3541 agagcgcacc gccgagttcg ccgccctcgc tgcttgccat gaattccttg tacttctccg
     3601 tcacgccgac gctcgccgtc gccttggtga cgatgggctt gatgcgggcg gcgagcgagg
     3661 gaccggcgac
//
LOCUS       DASUJG010000209         2441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1775242, whole genome shotgun sequence.
ACCESSION   DASUJG010000209 DASUJG010000000
VERSION     DASUJG010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1775242"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VFX38_04480"
     CDS             <1..563
                     /locus_tag="VFX38_04480"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX5314152.1"
                     /translation="GHFQDAGLWEARSAVRIPVIGLGESAMLHACTLGQTIGLVTIHP
                     LFISVHREQIRRYGLEHRVTAVVAVDSGAIDYVRAFSDPQAANALAQEYGRKIELLLD
                     QGCEVVLPAGGLPSLLFGAELKLSARAACVLDSTAVAIKSAAMAIDLQRFNGTAPSRI
                     GAFIPPTDAALAAVGMPRQAADAAGS"
     gene            547..1731
                     /locus_tag="VFX38_04485"
     CDS             547..1731
                     /locus_tag="VFX38_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX5314153.1"
                     /translation="MPQAASRIRAGYGGVAVTTPVTFGYAKRSTHGVHWHLGGVLREL
                     LRRSGIDKGDIDGLIVASYMLIPDHAATLAEYFGMSPRLALDVRQGGASGVIALRHAA
                     RAVQSGDADVVACIAGDVPPQDNSFTANFSTFARDHVFPHGAGGPNVVFALITANYMR
                     QTGARAEDFGRLCVAQRTNGSHFPGALLSTPLTLEEYLAARPISEPLRLYDCVMRCCG
                     AEGFLVMSEERASRLNIPWAGIAAAIERHNGAPEEPVQEGVEIAPDRDELWRQASLGP
                     RDMRFVQAYDDYPVIVMMQLESLGFCGKGEAARIVRERDLTVDGDLPLNTNGGMLALG
                     QAGAAGGFVGMTEGLRQVTGQALGRAVRGATAGLVSGYGTVNYDRGICSAAAVLRSGK
                     LA"
     gene            2208..>2441
                     /locus_tag="VFX38_04490"
     CDS             2208..>2441
                     /locus_tag="VFX38_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5314154.1"
                     /translation="MDSLIAAAARALAAGDALGALKRVALREDPPALALRGIAMAQLG
                     EHRRARELLREAARGFGVHEELARARCVVAEAEV"
BASE COUNT          391 a          785 c          825 g          440 t
ORIGIN      
        1 tcggccactt tcaggatgcc ggcctttggg aggcccgatc tgccgtgcgc atcccggtga
       61 tcggcctcgg cgagtccgcc atgctgcacg cctgcaccct tgggcagacg atcggtctcg
      121 tgacgattca tcctcttttc atatccgtgc accgagagca gattcgccgc tatggcctcg
      181 agcatcgggt gaccgccgtg gtcgccgtcg actccggtgc gatcgactat gtccgagcgt
      241 tttccgatcc gcaggctgcg aacgcgctgg cccaggaata cggccggaaa atcgagctgc
      301 tgctcgatca ggggtgcgaa gtggtgctcc cggcgggcgg actgccgagc ctgctctttg
      361 gcgcggaact caagctctcg gcccgcgcgg cctgcgtgct cgacagtacg gcggtggcaa
      421 tcaagtccgc cgcgatggcg atcgacctgc aacgcttcaa cggcaccgcg ccaagccgga
      481 tcggcgcctt catcccgccg acagatgccg cgctcgcggc cgtcggcatg ccgcggcagg
      541 cggcggatgc cgcaggcagc tagccgcatt cgcgccggat acggcggcgt tgcggtcacc
      601 acgccggtca cgttcggcta tgcgaagcgg tcgacgcacg gcgtccactg gcacctgggg
      661 ggtgtgttgc gcgaactgct gcgccgctcc ggcattgaca agggggacat tgatgggctg
      721 atcgtcgcga gctacatgct catacccgat cacgccgcca cgctcgccga gtacttcgga
      781 atgtcaccga ggctcgcgct cgatgtgcgc cagggcggcg catcaggcgt gatagcgctg
      841 cggcacgccg cgcgcgcagt ccagtccggc gacgcggacg tcgtcgcttg catagccggc
      901 gatgtgcctc cgcaggacaa ttcgtttaca gccaactttt cgaccttcgc tcgcgaccac
      961 gtcttcccgc acggcgctgg cgggcccaat gtcgtcttcg ccctcattac ggcgaactac
     1021 atgcgccaga ccggggccag ggcggaggat ttcgggcgct tgtgcgtagc tcagcgaacg
     1081 aacggctccc atttccccgg cgcattgctc tccaccccgc tcacgctcga ggaatacctc
     1141 gccgcgcggc ccatcagcga accgctgcgg ctctacgatt gcgtcatgcg ctgctgcggg
     1201 gccgaaggat tcctggtcat gagcgaagag cgtgcgtcgc gcctgaatat cccctgggcc
     1261 ggcattgccg ccgccattga acggcacaac ggggcgcccg aagagcccgt gcaggaaggc
     1321 gtagaaattg cgccagaccg ggacgagctt tggcgacagg cttctctcgg accgagggac
     1381 atgcgcttcg ttcaggccta cgatgattac ccggtgatag tcatgatgca gctcgaatcc
     1441 ctggggttct gcggcaaagg cgaggcggcc cggatcgtgc gcgagcgcga tctcaccgtc
     1501 gacggagatc tgccgctcaa caccaacggc ggcatgctcg cccttggcca ggcaggcgcg
     1561 gcgggcggtt tcgtgggcat gactgaaggg ctgcgacaag tgacagggca ggcgctgggc
     1621 cgcgcggttc gaggtgcgac agcgggcttg gtaagcggat acggaacggt caactatgac
     1681 cgcggcattt gctcggcggc cgcggttctc agatcgggga aactcgcatg aacctcccca
     1741 cagcggcggc cggcgggcgc ttcgggctcc ccgtgtgcga cgattgcggg acggtgcagt
     1801 atccgatacg agaagtctgc aagcgctgcc tttcggagcg actgagttgg tccgctctaa
     1861 agccgcaagg cgtgctgttg gcctgtacgc ggcttcaccg ctcgatggac gagcgcttcg
     1921 cgcccagact gcctttggaa atcggatctg tgaaattgga tgccggaccc gtagttatcg
     1981 cgttactgga tggccaatgc gaggttggca ggcccgtact gctaagtctc acgacgggtt
     2041 cagcgggcaa gaacattctt ctcgcatccc gcggcgaact gcaggacggt aaattgaatt
     2101 cctagggatt caagtggctc gggcccggaa gtagttttct acttgagccc atccgcagcg
     2161 gatatcgttc tactagtaat tgaccggttg cccgtaacca tgtcctcatg gactccctga
     2221 ttgccgcggc ggcgcgcgcg ctcgcggcgg gcgacgcgct cggcgcgctc aagcgggtcg
     2281 cgctgcgcga ggacccgccg gcgctcgctt tgcgcggcat cgcgatggcg cagctgggcg
     2341 agcaccggcg tgcgcgcgag ctgctgcggg aggccgcccg cggtttcggt gtccatgagg
     2401 agctggcgcg cgcgcgctgc gtcgttgccg aggccgaagt g
//
LOCUS       DASUJG010000210         3119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_430440, whole genome shotgun sequence.
ACCESSION   DASUJG010000210 DASUJG010000000
VERSION     DASUJG010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430440"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VFX38_04495"
     CDS             <1..627
                     /locus_tag="VFX38_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3417 domain-containing protein"
                     /protein_id="HEX5314155.1"
                     /translation="GREHADFAFTDRREAEALYDLLEDHVIPEFYERDSQGIPRRWVA
                     RIRASMSALTPQYSSNRMLREYLEHCYRPMAERFVHRAADGAAVARAVNEWLARIDER
                     WAQLRMEGPRFTPGAEGTAVEVHAYLDGLDPADVAIELYAEPRAAGEAPERVALERRE
                     ALVGATAGYRYEARLATGRPLEEYTLRLRPDNPDVLWPLESAKVLWQR"
     gene            complement(834..1862)
                     /gene="waaF"
                     /locus_tag="VFX38_04500"
     CDS             complement(834..1862)
                     /gene="waaF"
                     /locus_tag="VFX38_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006746105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEX5314156.1"
                     /translation="MSEQRILIAAPNWIGDFVIADTLFRLLKQREPRTAIDVLAPAWA
                     LPLAERMTTVAQAIELPGGHGELALAARLRLARRLRTVSYVRAYVLPRSAKSALVPWL
                     GRVPLRTGYRGEYRYGLLNDLRQLNPAHESLARRYAALAFAADAELPAELPPPVLASS
                     AATRAAAMSRLGLAPGAAPVVALAPGAEYGPAKRWPIEAYAALAARFAAAGWQSWLLG
                     SAGERLLAESVAARVPMARNLAGETTLVEAVDVLGASTVAVTNDSGLMHMAAALGIPQ
                     VALFGSSSPRYTPPANPRARILYRGLPCSPCFARECPLGHRNCLRAIGVEEVYLAARE
                     QARLAVAD"
     gene            complement(1859..2803)
                     /gene="rfaD"
                     /locus_tag="VFX38_04505"
     CDS             complement(1859..2803)
                     /gene="rfaD"
                     /locus_tag="VFX38_04505"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEX5314157.1"
                     /translation="MIVVTGGAGFIGSNLVHALNARGISDILVVDDLADGHKFTNIAA
                     AAIRDYEDRDRFLAAVEDERFAGRIEAIFHQGACSDTTEWDGRYLMDVNYRYSRVLLE
                     FAIARGVPFYYASSAAVYGAAAGFSESPENERPINLYGYSKTLFDRYVRRRLPQARSP
                     VAGLRYFNVYGPGEAHKGRMASVAWHFHRQIVESGRCRLFAASGGYGDGEQRRDFVYV
                     DDVVAINLWFLDHPECSGIYNAGSGRSQSFNDVARAVIAHHGRGQIEYVPFPADLEGS
                     YQNFTEADLSRLRACGCTLPFRAVESGVADYLGRLGDG"
     gene            complement(2800..3039)
                     /locus_tag="VFX38_04510"
     CDS             complement(2800..3039)
                     /locus_tag="VFX38_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEX5314158.1"
                     /translation="MYHGPDPKRTQIPNDRRQILVHEADLPLHCPLPAMSLWNSHPQV
                     YIPLEQPGQTAVCPYCGTEFRLAGEGAGGNEAGTR"
BASE COUNT          562 a         1040 c         1038 g          479 t
ORIGIN      
        1 ggtcgcgagc acgcggactt cgccttcacc gaccggcgcg aggccgaagc cctctacgat
       61 ctcctcgaag atcacgtgat cccggagttc tacgaacgcg acagccaggg aattccgcgt
      121 cgttgggtcg cgcgaatccg cgcgagcatg agcgcgttga cgccgcaata ttcctccaat
      181 cgcatgttgc gcgagtatct cgagcactgc tatcgcccga tggcggagcg attcgtgcat
      241 cgggccgccg acggtgccgc cgtcgcccgc gccgtgaacg aatggctcgc gcggatcgac
      301 gagcgctggg cacagttgcg catggaggga ccgcgattca cgccgggcgc ggaaggcact
      361 gcagtcgagg tgcacgccta cctcgacggc ctggatccgg cagacgtagc gatcgagctg
      421 tatgccgagc ccagggcggc cggtgaagct cccgaacgcg tcgcgcttga acgacgcgag
      481 gcgctggtcg gggccaccgc agggtatcgc tatgaggcac gacttgccac ggggcgcccg
      541 ctcgaggaat ataccctgcg gctgcgtccc gacaaccccg atgtcctctg gcctctcgaa
      601 tccgcgaagg tgctctggca acgctgagac cgccggagac cggggccggg catctggtcc
      661 gcggggaatt gcgacggatg ggaacttttc gcgatttgca gtatcctatt ctccgaacgc
      721 tgcttctacg cggcgttcat ttccgctctc cccctccctg agcggaaacc cggcaacccc
      781 aagccggaaa aggactggcc cggcgtctgc aaagacgccg ggtctttttt ttgtcagtcc
      841 gccaccgcaa gccgagcctg ctcccgcgcg gcgagataca cctcctcgac gccgatcgcg
      901 cgcagacagt ttcgatgccc gagcggacat tcgcgtgcaa agcagggact gcaaggcagg
      961 ccgcgataga ggatcctcgc gcgcgggttg gcgggcgggg tatagcgcgg actcgaggaa
     1021 ccgaacaggg cgacctgcgg aattcccagg gctgcggcca tatgcatgag cccggagtcg
     1081 ttggtcaccg cgaccgtaga ggcgcccagc acatccacgg cctcgacgag ggtcgtttca
     1141 ccggcgagat tgcgcgccat cggaacccgc gcagcgaccg actcggctag gagacgctct
     1201 ccggcgctcc cgagcagcca ggattgccag cccgcggccg cgaatcgtgc cgccagggcc
     1261 gcataggctt cgatcggcca gcgcttggcg gggccgtatt cggcgcccgg agccagggcc
     1321 acaaccggcg cggcgcccgg cgcgagaccg aggcgtgaca tcgctgcggc gcgcgttgcg
     1381 gcgctgctcg ccagcaccgg cggcggcaac tctgccggca gttccgcatc cgcggcaaag
     1441 gcgagggccg cataacgccg ggcgagcgac tcgtgcgcgg ggttcagctg ccgcaggtca
     1501 ttgagaagtc cgtaacggta ctcgccgcga tagcccgttc gcagcggcac tcgaccgagc
     1561 cagggcacca gcgccgactt ggccgaacgc ggcagcacat aggcgcgcac gtaggagacg
     1621 gtccgaagcc gccgagccag gcgcaggcgg gcggcaaggg cgagctcccc atgccccccc
     1681 ggcaattcga tcgcctgcgc aaccgtcgtc attcgctcgg caaggggcag cgcccacgcc
     1741 ggcgccagca cgtcgatcgc ggtccggggc tcgcgctgct tgaggagccg aaaaagcgtg
     1801 tcggcgatca cgaaatcgcc gatccagttc ggagcggcga tcagaatccg ctgctcgctc
     1861 atccgtctcc gaggcgcccg agatagtcgg ccacccctga ctcgaccgcc cggaagggaa
     1921 gcgtgcagcc gcaggcgcgc aggcgggaga gatcggcctc ggtaaagttc tgataactac
     1981 cctcgagatc ggccgggaag ggaacatatt cgatctggcc gcgcccgtga tgggcgatca
     2041 ccgcccgcgc gacgtcgttg aaactctggc tgcgcccgct cccggcgttg tagatgccgg
     2101 agcattcggg atgatccagg aaccagaggt tgatcgcgac gacgtcgtcc acgtacacga
     2161 aatcgcggcg ttgctcgccg tcgccatagc cgccgctcgc ggcaaacagg cgacaacggc
     2221 cggattccac gatctgacga tggaaatgcc aggcgacgct cgccatccgg cccttgtgcg
     2281 cctctccggg gccatagacg ttgaaatagc gcaaacccgc aaccggggag cgcgcctgcg
     2341 gcaggcgccg acgcacgtag cgatcgaaaa gcgtcttcga atagccgtag agattgatcg
     2401 ggcgttcgtt ttccggactt tcggaaaatc ccgccgccgc gccatacacc gccgccgacg
     2461 aggcgtaata aaaggggacg ccgcgcgcga tcgcgaattc gaggagcaca cgcgagtaac
     2521 gatagttcac gtccatgaga tagcgcccgt cccattcggt agtgtccgag caggcgccct
     2581 ggtgaaaaat cgcctcgatc ctcccggcga agcgctcgtc ctcgacggcg gcgagaaaac
     2641 ggtcccgatc ctcgtagtca cggatcgcgg cggcggcgat gttcgtgaac ttgtggccgt
     2701 ccgcgagatc gtccacgaca agaatgtccg agatgccgcg ggcgttgagc gcgtggacca
     2761 ggttcgagcc gatgaagccc gcaccgcccg tgacgacgat cacctggtgc cggcctcatt
     2821 ccccccggcg ccttcgccgg cgagccggaa ttccgttccg caatacgggc ataccgctgt
     2881 ttgacccggt tgctcgagcg ggatatacac ctgcggatgg gagttccaca ggctcatcgc
     2941 cggcagcggg caatgcaggg gaaggtcggc ttcgtgcacc aggatctgcc ggcggtcgtt
     3001 cggaatttgc gtgcgcttcg ggtccgggcc gtggtacatc ggagtttcgg cgtgcaaccg
     3061 gcttcattat agcggcgcat cgccgaagac ttcgcgccac aaccggacga cgtgatccc
//
LOCUS       DASUJG010000211         2676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_186691, whole genome shotgun sequence.
ACCESSION   DASUJG010000211 DASUJG010000000
VERSION     DASUJG010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186691"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..1110
                     /gene="lpxK"
                     /locus_tag="VFX38_04515"
     CDS             49..1110
                     /gene="lpxK"
                     /locus_tag="VFX38_04515"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEX5314159.1"
                     /translation="MTKKLRECVAKDLQARWYRSRAPWFLLPPAGLFGAAAALRRTLY
                     AHGMLKSVRVSARVVVVGNIAVGGSGKTPLVAWLAQRLAADGLRVGIVTRGYGGYDRG
                     PRQMTADTSVEAAGDEALWLARTSGVPVVAGRDRVAAARLLVETAHPEIILSDDGLQH
                     YRLARDAEVVAVDARRGYGNGALLPAGPLREPLRRIASAQAVVLKGEGNATLPAEVRA
                     FRMHHALGEALPLAGGEPRPLSAFAGTPVRALAAIADPEGFFRDLEGAGLEVERHALP
                     DHAPVADAVARLPADRPLLMTDKDAVKLAAPPAHAWRVPLVPRFEESDANALLAIVRG
                     TISRPEEFSVHSTPTHPGD"
     gene            1113..1295
                     /locus_tag="VFX38_04520"
     CDS             1113..1295
                     /locus_tag="VFX38_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX5314160.1"
                     /translation="MDSTLLDILVCPLCKGPLQYDKAAQELICRAERLAFPIRDDIPV
                     MLEEEARQVPTEELER"
     gene            1292..2050
                     /gene="kdsB"
                     /locus_tag="VFX38_04525"
     CDS             1292..2050
                     /gene="kdsB"
                     /locus_tag="VFX38_04525"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEX5314161.1"
                     /translation="MSFRVAIPARYGASRLPGKPLVKIAGKPLIVHVCERALASAAAE
                     VWVATDDERIAEACAGLDVKIALTSAAHASGTDRVAEVAAREAWAEDDIVLDLQGDEP
                     LMPAMAIDAAAASLASDPRAAMATLAVRLFDVEAFRDPHVVKVVTDCSGNALYFSRAP
                     LPRPREAASGVPEQTLRHVGLYAFRVATLARFALEPPCDLERAEGLEQLRALWLGMRL
                     RVEVVADIPMAAVDTPEDVKQVEDLLAAEREARA"
     gene            2047..2514
                     /locus_tag="VFX38_04530"
     CDS             2047..2514
                     /locus_tag="VFX38_04530"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007467210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEX5314162.1"
                     /translation="MTRVLFICMGNICRSPLAEAALRFRARERHIAVEARSAATHSWH
                     VGEGAEARAIAAGRRRGYLLSGHRARQAVAGDFLQEEFILAMDRGNLARLRRFAPART
                     SARIGLLLDYAEETDTNEVPDPYYGGEQVFERALDLIDTAVDGFLRRLAAGPS"
BASE COUNT          406 a          950 c          899 g          421 t
ORIGIN      
        1 ctgcgcttat gcaggcctac gtcacacgag atacggaaag ctgagcgaat gacaaaaaaa
       61 ctgcgcgagt gcgtggcgaa ggatctgcaa gcacgctggt acaggtcgcg ggcgccgtgg
      121 tttctgctgc cgccggccgg actgttcggc gccgctgccg cgctgcgccg cacactgtat
      181 gcgcacggca tgctgaaatc cgtgcgtgtc tcggcccgcg tcgtcgtggt cggcaacatc
      241 gccgtcggcg gcagcggcaa gacgccactc gtcgcgtggc tggcgcaacg cctcgcggcc
      301 gacggcctgc gggtcggtat cgtcacccgc ggctacggcg gatacgatcg cgggccgcga
      361 caaatgacgg cggacacatc ggtcgaggcc gcgggcgacg aggcattgtg gctcgcgcga
      421 acgagcggcg taccggtggt cgcgggccgc gatcgggtcg ccgccgcacg gctcctcgtc
      481 gaaaccgcgc atcccgaaat catcctgagc gacgacggct tgcagcatta tcgcctcgcc
      541 cgcgatgccg aagtcgtcgc cgtggatgcg cggcgcggct acggcaacgg cgcgctgctt
      601 ccagccggcc cgctgcgcga accgctgcgg cgcatcgcct ccgcgcaagc ggtcgtgctg
      661 aagggcgaag ggaatgccac acttccggca gaagtgcggg cgtttcgcat gcaccacgcc
      721 ctcggcgaag cccttccgct cgcgggcggt gaaccgagac cgctctcggc cttcgcggga
      781 actccggtgc gggcgctcgc ggcaatcgcg gatcccgaag ggtttttccg ggatctcgaa
      841 ggcgccgggc tcgaagtcga acgccacgcg ctgccggatc atgcgccggt tgccgatgcc
      901 gtcgcgcggc ttcccgccga ccgcccgctc ctgatgaccg acaaggatgc ggtcaaactc
      961 gccgcgccgc ccgcccacgc ctggcgggtg ccgctggttc cgcgcttcga ggagtcggat
     1021 gcgaacgcac tgctcgcgat cgtccgcggt accatctccc ggcccgaaga attttccgta
     1081 cactcgacac ctacgcatcc cggagactga cgatggattc caccctgctc gacattctgg
     1141 tctgcccgct ctgcaagggc ccgctgcaat acgacaaggc cgcgcaggaa ctgatctgcc
     1201 gcgccgagcg cctcgccttt ccgatccgcg acgatatccc cgtaatgctg gaggaggaag
     1261 cgcgccaagt accgacggaa gaactcgagc ggtgagcttc cgcgtcgcca tcccggcgcg
     1321 ctacggcgcg agccgcctgc ccggcaagcc gctggtgaaa atcgctggca agccgctcat
     1381 cgtccacgtc tgcgagcggg cactcgcgag cgcggccgcc gaggtctggg tcgcgaccga
     1441 cgacgagcgc atcgccgagg cctgcgcggg ccttgacgtc aaaatcgcgt tgacctccgc
     1501 ggcgcacgct tccggtaccg atcgcgtcgc cgaggtcgcc gcccgcgaag cctgggcgga
     1561 agacgacatc gtgctcgatc ttcagggcga cgaacccttg atgccggcaa tggcgatcga
     1621 tgccgccgcg gcctcgctcg cgtccgatcc gcgggcggcg atggcgacac tcgccgtgcg
     1681 gctgttcgac gtcgaggcct ttcgcgaccc ccacgtggtg aaggtcgtaa ccgactgctc
     1741 cggcaacgcg ctgtatttca gccgcgcccc gctgccgcgc ccgcgcgagg ccgcgagcgg
     1801 agtcccggaa caaaccctgc gtcatgtcgg gctctacgcc tttcgcgtcg cgacgctcgc
     1861 gcggttcgcg ctcgagccgc cctgcgatct cgagcgcgcc gaaggactcg agcaactgcg
     1921 cgcgctgtgg ctggggatgc gcctccgggt cgaggtggtc gccgacattc cgatggccgc
     1981 cgtggacacg ccggaggatg tgaagcaggt ggaagacctg ctcgcggcgg agcgcgaggc
     2041 gcgggcgtga cccgggtgct cttcatctgc atgggaaaca tctgccgctc gccgcttgcg
     2101 gaagccgcat tgcgattccg cgcacgtgag cgccacatcg ccgtcgaggc gcgatccgcg
     2161 gccacccatt cctggcacgt gggcgaaggg gcggaggccc gggcgatcgc ggccggtcgt
     2221 cgacgcggct atctgctctc cggacatcgc gcaagacagg cggtcgccgg cgacttcctg
     2281 caggaagaat tcatcctcgc gatggatcgc ggcaatctcg cgcggctgcg acgcttcgcc
     2341 ccggcccgaa ccagtgcgcg catcgggctt ctgctcgact atgccgagga aaccgataca
     2401 aacgaagtgc ccgaccccta ctacggcgga gagcaggtct tcgaacgtgc tctcgacctg
     2461 atcgacaccg ccgtcgacgg atttcttcgg cgcctggccg cagggccttc ctagcagcga
     2521 atccggccgc cgccgggccg gacgcttcgc gcctcagacc ttctcgctct ctcccgcttc
     2581 cgctcccgcc tgcgcctgct cgcgctcgta cagtgcagcc aatctcggcg tttcgcgtgg
     2641 tgggttgtcg ggagtcgctt ccgcccgtga cggcac
//
LOCUS       DASUJG010000212         3073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1164303, whole genome shotgun sequence.
ACCESSION   DASUJG010000212 DASUJG010000000
VERSION     DASUJG010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164303"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /locus_tag="VFX38_04535"
     CDS             complement(<1..167)
                     /locus_tag="VFX38_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HEX5314163.1"
                     /translation="MPAVTVRNLPAETHRALKLRAARHGRSTEAEIRAILEDATIAEN
                     RLKIGSALAGFR"
     gene            383..964
                     /locus_tag="VFX38_04540"
     CDS             383..964
                     /locus_tag="VFX38_04540"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5314164.1"
                     /translation="MAAATELTWQKGYNAVTVDAICERAGVKKGSFYHFFESKAALAL
                     AAMENAWQTRSRPNLDAAFSAGNPPLERLKRFFDALYQVQVDEMSREGRVLGCPFFSL
                     GMESGGLEPEVWEASRGYVKRKRRYLESAIRDAQAEGLVDIPSVEEAAEGVHSLLEGA
                     LARSRVQNDPAPLRELYGQALCLLGVRHPVTEK"
     gene            1045..2289
                     /locus_tag="VFX38_04545"
     CDS             1045..2289
                     /locus_tag="VFX38_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008929033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5314165.1"
                     /translation="MSQAKQTETRLPPAPEIRRKRRRRLALLGLAALFLVVGGSYGAY
                     WALVGRFEQSTDDAYVAGNRVAVMPQEEGTVVAILADDTSRVHRGEILVRLDDSNARI
                     ALQQAKANLAATVRATNALYATEKQLQAKVAKQQATLELARHDYARDEDMHKLGYYST
                     KNLQHSETLVDVDKRSLAAAEQQLEAVRARVRDGGVADNPEVRLAAAKLRAAWLAVQR
                     ARIVAPVSGYVAERDVQLGEDVAPGTALMAIVPLDQLWVEANFKESQLASVRPGQTVT
                     MQADLYGGSTTFHGRVIGIGAGTGSAFSLLPPQNATGNWIKVVQRVPVRIGIAKADLA
                     RHALRIGLSVNVTVETARNDRTKVGPIVAPHTYKTSVYEAQASGADRLIANIIRDNLG
                     VAVGGDVARARARPEAGSSHGQ"
     gene            2279..>3073
                     /locus_tag="VFX38_04550"
     CDS             2279..>3073
                     /locus_tag="VFX38_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006913977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX5314166.1"
                     /translation="MGSKYPPITGGRLALLTVGVSLALFMNVLDTSIANVAIPTIAGN
                     LAVSADEGTWVITSFAVSLAIALPLTGWLGKRFGEVRLFLAATLLFSLFSLLCGLAVS
                     LPMLIVCRVLQGAVAGPMIPLSQSLLLANYPPEKQATALSIWGMIAVVAPVVGPVLGG
                     MVTDDFSWPWVFYINVPVGIVAVAITAFILRGRETDKERRPIDKIGLGLLVVGVASLQ
                     IMLDKGNELSWFSSSTICWLAAIAVVALSFFVVWELTEANPVVDLTL"
BASE COUNT          540 a          985 c         1002 g          546 t
ORIGIN      
        1 cggaatcccg caagcgccga tccgattttg aggcgattct ccgctatggt cgcatcttcc
       61 aggatggcgc ggatctccgc ttcggtactc cgcccatggc gagcggcgcg cagtttcagc
      121 gcacgatggg tctccgccgg caaattgcga acggtgacgg ctggcatctg cttcgacctc
      181 gaacggattc cggtgatgat gctatcacaa cagcgggatt gatagcacgg aagacaaagg
      241 cgcggtcgaa gcgcggacgc cggccgctca gcccccgata aattggcaga acgcgccgga
      301 cgcggcgccg ggacttgaaa ttgactgaac ggtcaactat actgcggcca tgggtcgaac
      361 gagcgatgcg cgggaacggc tgatggcggc cgccaccgag ttgacctggc agaaagggta
      421 caacgcggtg acggtggacg cgatttgcga gcgcgcgggc gtgaagaagg ggagcttcta
      481 ccatttcttc gagtcgaagg ccgcgctggc gcttgcggcc atggaaaacg cctggcagac
      541 gcgcagccgc cccaatctcg acgcagcgtt ctctgcgggc aacccgccgc tggagcggct
      601 caagcggttc ttcgacgcgc tttaccaagt ccaggtcgac gagatgagca gagaagggcg
      661 cgtattgggg tgcccgtttt tcagcctcgg catggaaagc ggcgggctgg aaccggaggt
      721 ctgggaagcg tctcgtggct atgtgaaacg caagcgccgc tatctcgagt ccgcgatccg
      781 tgacgcgcag gccgaagggc tcgtcgacat cccgagtgtg gaagaggccg cggaaggggt
      841 gcacagcctg ctcgaagggg cgctcgcacg ctcacgggtg cagaacgatc cggcgccgct
      901 gcgggaactc tacggacagg ccctgtgcct gctcggagtc cgacacccgg ttacggagaa
      961 gtaagccgcc gcgacggcgg ctgcgggatt ttctcttttt ttatcagcaa cttgactagt
     1021 tagtcaacta ttaatggagt tttcatgagt caagccaaac aaaccgaaac caggctcccg
     1081 ccggctcccg agatccgccg caaacgccgg cgccgcctcg cgctcctggg tctcgcggcc
     1141 ttgttcctcg ttgtcggcgg aagctacgga gcttactggg cgcttgtcgg gcgcttcgag
     1201 caatccaccg acgatgccta cgtcgccggc aaccgcgtcg cggtgatgcc gcaggaggag
     1261 ggcacggtgg tcgcgatcct tgccgacgac acttcgcgag ttcatcgcgg cgaaatcctc
     1321 gtgcgcctgg acgacagcaa tgcgcgcatc gcattgcagc aggcaaaggc caatctcgcc
     1381 gccacggtgc gggcgaccaa cgcgctctac gcgacggaaa agcagttgca ggccaaggtc
     1441 gcgaagcagc aggcgacgct cgaactcgca cgccacgact atgcccgcga cgaggatatg
     1501 cacaagctcg gctactactc gaccaagaac ctccaacaca gcgaaacgct cgtggacgtg
     1561 gacaagcgca gtctcgccgc cgcggagcag caattggagg cggtgcgggc gcgggtccgc
     1621 gacggcggcg tcgccgacaa tcccgaagtg cgacttgccg ctgccaaatt gcgcgccgcc
     1681 tggctcgcgg tccaacgggc gcggatcgtg gcgccggtct cgggttacgt cgccgagcgc
     1741 gacgtccagc ttggcgaaga cgtcgcgccg ggcacggcgc tgatggcaat cgtgccgctc
     1801 gatcaactct gggtcgaagc caacttcaag gaatcgcagc ttgcgagcgt tcgacccggc
     1861 cagaccgtga ccatgcaggc ggatctctac ggcggttcga cgacgtttca cggccgcgtg
     1921 atcggcatcg gcgcgggaac cggaagcgcg ttttcgctgc tcccgccgca gaacgctacg
     1981 ggaaattgga tcaaggtcgt gcagcgcgtg ccggtgcgga tcggcatcgc gaaggccgat
     2041 ctcgcacgcc atgcgctgcg catcggcctg tcggtgaacg tcacggtcga gaccgcccgc
     2101 aacgacagga cgaaggtggg cccgatcgtc gctccccaca cgtacaagac ttccgtgtac
     2161 gaagcgcagg cctccggcgc cgatcgtttg atcgcgaaca tcattcgcga caatctcggc
     2221 gttgcggtcg gcggcgacgt tgcgcgcgcg cgcgcgaggc ccgaagccgg atcctcgcat
     2281 gggcagtaag tatccgccga ttaccggcgg caggttggcg ctcctcaccg tcggcgtatc
     2341 gctcgcgctc ttcatgaacg tgctcgacac ctcgatcgcg aacgtcgcga tcccgacgat
     2401 cgccggcaat ctcgccgtca gcgccgacga gggcacctgg gtgattacct ccttcgcggt
     2461 cagcctcgcg atcgcgctgc cgctcaccgg ctggctcgga aagcgcttcg gcgaagtgcg
     2521 tctgttcctc gccgcgacgc tgttgttctc gctcttttcg ctgctctgcg gtctcgcggt
     2581 ttcgctgccg atgctgatcg tctgccgcgt gctgcaaggt gcggtcgcgg gtcccatgat
     2641 tccgctgtcg caaagcctgc tgcttgcgaa ctacccgccg gaaaagcagg cgaccgcgct
     2701 ttcgatctgg ggcatgatcg cggtcgtcgc gccggtcgtg gggcccgttc tcggcggcat
     2761 ggtcaccgac gacttcagtt ggccctgggt cttctacatc aacgttccgg tcggcatcgt
     2821 cgcggtcgcg atcaccgcct tcatcctgcg ggggcgcgaa accgacaagg agcgccgccc
     2881 gatcgacaag atcggccttg gtctcctcgt cgtaggcgtc gcaagcctcc agatcatgct
     2941 cgacaagggc aacgaacttt cctggttttc ctcgtccacc atctgttggc tggctgcgat
     3001 cgcggtcgtc gccctgagct tcttcgtggt ctgggagctt acggaagcca atcccgtggt
     3061 cgatctcacg ctc
//
LOCUS       DASUJG010000213         2173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_920982, whole genome shotgun sequence.
ACCESSION   DASUJG010000213 DASUJG010000000
VERSION     DASUJG010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920982"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VFX38_04555"
     CDS             complement(<1..485)
                     /locus_tag="VFX38_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314167.1"
                     /translation="MRLRLRIPATNTRGPVYTDQALAALHETLSQKTCLTLSISATTD
                     AVAIGCACPPDLRSVLESQLYAQYPDCRLDAVDAATPDATRTEWTADVGVRRDLFPIK
                     RYPQFEDALNRQTADPLTAILTAATPAHESQLESRVTLCLRPAAHRKRSRAEKAHRKL
                     TT"
     gene            complement(798..1649)
                     /locus_tag="VFX38_04560"
     CDS             complement(798..1649)
                     /locus_tag="VFX38_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314168.1"
                     /translation="MQRRIVTDRDIEILTALDRVPLTAQQMLAVSETFSHPFGSERTV
                     RERLQAMSDAGWIRSSLYALPSSGGSPKYYFLTRQGYGILNDPEAPPPTKRHFSPLSI
                     SRQHHTKALADFLVHMLRASHRSNVRFTEFYRENTLRLTVGEESLYPDSSFQLIVENR
                     PLNFVVEIDNHTETIRSAKDTETWERKLRLYDRLQDSIENRFRVLAITTRSTDRLRHI
                     LMTAAGVLKNPQRSLVYGIDLPTFIREEEPLHRSVFRDHFGRATALIASHFSIRRLHR
                     AAQPAVI"
     gene            1745..>2173
                     /locus_tag="VFX38_04565"
     CDS             1745..>2173
                     /locus_tag="VFX38_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314169.1"
                     /translation="MNVDELMPQLSVEQVALFYGVQLPDATRIGNETRMQCFLACGKA
                     HETGDRALAIQTDHPAKQWKCHQYGCGKGGNLVSMCDLLKPGTNLNGRPRGQRFKEIA
                     SDIRKMVAGVFDAPRPAEVKTVAAAAPPPPKVNLPLVKSEN"
BASE COUNT          382 a          612 c          777 g          402 t
ORIGIN      
        1 gtcgtgagct tgcgatgcgc cttttccgcg cgcgagcgct tgcggtgggc tgccgggcgc
       61 aggcaaagcg tcacgcgcga ttcgagctgg ctctcgtgtg cgggcgtcgc ggcggtcaag
      121 atcgccgtca gcggatcggc cgtctgccgg ttgagcgcgt cttcgaactg cggatagcgc
      181 ttgatcggaa aaagatcgcg ccggacccca acatcggccg tccactcagt gcgcgttgca
      241 tcgggcgttg cggcatcgac agcatcgagc cggcagtcgg gatactgggc atagagctga
      301 ctttcgagaa ccgaccgaag atccggcggg cacgcgcagc cgatcgcgac ggcatcggtc
      361 gtggcgctga tggagagggt gaggcacgtt ttctgcgaga gtgtttcgtg cagcgcggcg
      421 agggcctggt cggtgtagac ggggccgcgg gtgttcgtcg cggggatgcg gagacggagg
      481 cgcatgcggc ggagaatacc aagcggtgcg gcgctcgtct cttgtgccgc gacgcccttt
      541 ccggtgtggc gaccattcgt cggtttcccc gccccggcag cttgctcttc tgcacggggc
      601 ggggcttgcc cgaagacgcg ttggccgtga acccaatccg ggaggtggca aggattcctt
      661 gcgtcgccgg tgactcggcc gcggtatcgc cacaacgacg gccggctgtg ccggcgtcct
      721 ctggcggcgg agagggaagg tcgcgtggta gcgaacactt agaggcgcat ggagcgcact
      781 tccgacaagg cttcgcacta tatcacagcc ggctgggccg cgcgatggag tctgcgaatc
      841 gagaagtgcg aggcgatgag cgccgtcgcc cggccgaagt ggtcgcggaa aacgctgcgg
      901 tgcagcggct cctcctcgcg gatgaacgtc ggcaggtcga tgccgtagac gagcgatcgc
      961 tgggggtttt tcagaacgcc cgcggccgtc atcaggatat ggcgcaggcg gtcggtcgat
     1021 cgcgtggtga tcgcgaggac gcggaagcgg ttttcaatgg agtcctgcaa ccggtcgtag
     1081 agccgcagct tcctctccca cgtctcggta tctttcgccg atcggatggt ctcggtgtga
     1141 ttgtcgatct cgacgacgaa gttcagcggc cgattctcga caatgagctg gaaggaggag
     1201 tcggggtaga gcgattcttc gccgaccgtg agcctgagcg tgttctcgcg gtagaactcc
     1261 gtgaagcgga cgttcgatcg gtgcgaggct ctgagcatgt ggacgagaaa atcggcgagc
     1321 gccttcgtgt gatgctggcg agaaatactg agcggtgaaa aatgccgttt cgtcggcggg
     1381 ggtgcctcgg ggtcgttcag aatcccgtag ccctggcgag tgaggaagta atacttcggc
     1441 gagccaccgc tgctcggaag tgcgtagagc gacgatcgga tccatccggc atcgctcatc
     1501 gcctggagcc gctcgcgcac ggtgcgctcg gagccaaacg gatgcgagaa tgtctcgctc
     1561 acggcgagca tttgttgcgc ggtgagtggc acgcggtcga gcgcggtgag aatctcgatg
     1621 tcccgatcgg tgacgatgcg gcgctgcatt tgcgctggcg aagtgttgag agggagtata
     1681 gcatgatggc cggttaaccg cttgctcttc ggcttgcatg ggatccgaaa aacccgccgg
     1741 ctttgtgaac gtcgatgagc tgatgccgca gctttcggtc gagcaggtcg cgctcttcta
     1801 cggcgtccag cttccggatg caacgaggat cgggaacgag acgcggatgc agtgttttct
     1861 cgcgtgcggc aaggcgcacg agacggggga ccgggcactc gcaattcaaa ccgatcatcc
     1921 ggcgaagcaa tggaagtgcc accagtacgg ctgcggcaag ggcggcaacc tggtatccat
     1981 gtgcgatctg ctcaagccag gcacgaactt aaacggtcgg ccgcgcgggc agcggttcaa
     2041 ggagattgcc tcagacatcc ggaaaatggt ggccggcgtg ttcgacgctc cgcgtcctgc
     2101 cgaggtgaaa acagtcgcgg cggctgcgcc gcctccaccg aaggtcaacc taccgctcgt
     2161 aaagtccgag aac
//
LOCUS       DASUJG010000214         2283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1532545, whole genome shotgun sequence.
ACCESSION   DASUJG010000214 DASUJG010000000
VERSION     DASUJG010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1532545"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VFX38_04570"
     CDS             complement(<1..655)
                     /locus_tag="VFX38_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314170.1"
                     /translation="MRAAALASLARTLASRRMLVAALMGFSSGLPLLLTLTLLQAWLT
                     LRGVSLAVIGQFALTQLPYTFKFLWAPLLDRFHLGFLGRRRGWLLALQILVALAIVAL
                     GIVGVPDAGRSITTNPARAIAAMPFAPPLGALPSLATDYVHSLGALLPGLVATAIAGF
                     LLAFFSASQDVVIDAYRRESLHDDGEQSLGASLYVWGYRIATLFAGGGGLILAAHMPF
                     "
     gene            complement(655..1425)
                     /locus_tag="VFX38_04575"
     CDS             complement(655..1425)
                     /locus_tag="VFX38_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018860993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX5314171.1"
                     /translation="MRIISFNANGIRAAERKGFFLWLAAQDFDVCCLQELKARPEQLD
                     ERFHPPGFFTHYYCAERAGYAGVGLIARSKPDLVVPGCGFAEFDCEGRYLEARFGDLA
                     VVSLYLPSGSAGLLRQESKDRFLALFLPYLEKLRRRRREYLLCGDWNIAHKEIDLKNW
                     KGNRKNSGFLPHERAWLDEVFDRIGWVDAFRKVNQEPDQYTWWSQRGRAREKNVGWRI
                     DYQIATPGLGARTRSAEIYTGENLSDHAPLILDYDWPP"
     gene            1487..2176
                     /gene="pyrE"
                     /locus_tag="VFX38_04580"
     CDS             1487..2176
                     /gene="pyrE"
                     /locus_tag="VFX38_04580"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009723299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX5314172.1"
                     /translation="MASLASEKLAFIEFAQEAGVLRFGRFTLKSGRESPYFFNLGAVA
                     TGVGLARLGKFYARALAASGLEPDVIFGPAYKGIPLAAATAIAWAGEMDCDLPFAYNR
                     KEAKDHGEGGNLVGAPLAGKVVIVDDVITAGTAVREAIALICNAGARPAGLLIAIDRE
                     ERGRGEQSAVAELATEEGLAVIALITLSDVVAWLEESGEHARELEALAAYRARYGAEG
                     WSLIPAGPHQA"
BASE COUNT          422 a          728 c          782 g          351 t
ORIGIN      
        1 gaaacggcat gtgcgcggcg agaatcaggc cgccaccccc ggcaaagagc gtcgcgatcc
       61 gataccccca gacgtagagt gaggcgccga gactctgctc gccgtcgtcg tgcagcgatt
      121 cgcggcggta ggcatcgatc accacgtcct gcgaggcgga aaaaaaggcg agcagaaagc
      181 ccgcgattgc ggtcgcgacc agtcccggaa gcagagcacc gagcgaatgg acatagtccg
      241 tcgcgagtga aggaagcgcg ccgagcggcg gcgcgaaggg catggcggcg atggcgcgcg
      301 ccgggttggt cgtgatcgaa cgtccggcat cgggcacgcc gacgatcccg agagcgacga
      361 tcgcgagcgc gacgagaatc tggagtgcga ggagccagcc gcgtctgcgg ccgagaaatc
      421 ccaggtgaaa acggtcgagc agcggcgccc acaggaactt gaaggtgtag ggcaattgcg
      481 tcagcgcgaa ctgcccgatc accgcgagcg agactccgcg cagcgtgagc caggcctgca
      541 gaagcgtgag cgtgagaaga agcggcaggc ccgaggaaaa gcccatgagg gccgcgacca
      601 gcatgcggcg cgaggcgagc gtccgtgcaa ggctcgcgag cgcggcggcg cgcatcaggg
      661 cggccagtcg tagtcgagga tgagcggtgc gtgatccgag agattctccc cggtgtagat
      721 ttcggcgctc cgcgttcgcg cgccgagtcc cggggtcgcg atctgataat cgatgcgcca
      781 gccgacgttc ttctcgcgcg cccgtccgcg ctgcgaccac caggtgtact gatcgggctc
      841 ctgattcacc ttgcggaaag catccaccca accgatccga tcgaagacct cgtcgagcca
      901 ggcgcgttcg tgcggcagga agcccgagtt tttccggttg cctttccagt tcttgagatc
      961 gatctccttg tgcgcgatat tccagtcgcc gcaaagcaga tactcacggc gacgacgccg
     1021 aagcttttcc aggtacggaa ggaaaagcgc gaggaaacga tccttcgatt cctggcgcag
     1081 gagcccggcc gagcccgacg gcagatacag cgacaccacg gcgagatcgc cgaagcgcgc
     1141 ctcgagatag cgaccctcgc agtcgaattc cgcaaagccg cagccgggga cgacgagatc
     1201 gggtttgctg cgggcgatga gcccgacgcc cgcatagcct gcgcgctcgg cgcaatagta
     1261 gtgcgtgaag aagcccggcg gatgaaaacg ctcgtcgagc tgctcgggac gggccttgag
     1321 ttcctgcagg cagcagacgt cgaagtcctg cgccgcgagc cagaggaaaa aacccttgcg
     1381 ctccgccgcg cggatgccgt tggcgttgaa gctgatgatg cgcatggaaa tcccgccgcc
     1441 gctgacgggc gctatcataa cggccgtttt cccgccgcgt ttgccgatgg cctcgctcgc
     1501 ctccgaaaaa ctcgccttca tcgaatttgc ccaggaggcg ggcgtactgc gtttcgggcg
     1561 cttcacgctc aagtcggggc gcgagagccc gtatttcttc aacctcggcg ccgtcgctac
     1621 cggagtgggg ctcgcccgcc tcgggaagtt ttatgcccgg gcgctggccg cttccggcct
     1681 cgaaccggat gtgatcttcg gccctgctta caagggaatc ccgctggccg cggcgaccgc
     1741 gatcgcctgg gccggcgaga tggattgcga tctgcccttt gcctacaacc gcaaagaggc
     1801 gaaggaccac ggcgagggcg gcaacctcgt gggcgcaccg ctcgcgggaa aggtcgtgat
     1861 cgtggacgac gtcattaccg ccggcaccgc cgtgcgcgag gcgatcgcgc tgatctgcaa
     1921 tgccggtgcc cgtcccgccg gactgctgat cgcaatagac cgcgaggagc ggggacgggg
     1981 cgagcagagc gcggttgcgg aactggcgac cgaagaaggc ctcgccgtca tcgccctgat
     2041 cacactttcc gatgtcgtcg cctggctcga agagtccggc gaacacgccc gcgaactcga
     2101 agccctcgcc gcgtaccgcg cccgttacgg cgccgagggc tggagcttga tacccgcagg
     2161 cccgcatcag gcttaaggcc gctacgggcg ttgcccttgg gtgcaggtcg cccgggcggc
     2221 cgcgccgctt atgcgggtct tttaggtgct cccgatctcg tggcgggtgc gcgggctatc
     2281 atc
//
LOCUS       DASUJG010000215         4439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_675838, whole genome shotgun sequence.
ACCESSION   DASUJG010000215 DASUJG010000000
VERSION     DASUJG010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675838"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /locus_tag="VFX38_04585"
     CDS             <1..1104
                     /locus_tag="VFX38_04585"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX5314173.1"
                     /translation="LIVMPDKMSREKVFNVQAMGAEVVLTRSDVRKGHPEYYQDLAQR
                     LAGERGGYYINQFANVDNPAAHEATTGPEIFEQMDGRVDAIVLGVGTSGTLTGVSRYF
                     AKASPATEFVLADPEGSVLADYIDTGKIGEARRWLVEGIGEDFIPTIADLSAVKRAYT
                     ISDAESFATAREVLRKEGILLGSSSGTLIAAALRYCREQTEAKRVLTLGCDTGAKYLS
                     KFYNDFWLAERGFLVPESAGNLSDLITRPHRHHATVTVAPEDTLATAHDRLRDGGYSQ
                     LPVVREGRLVGAITEGDLLHAVIDLALPFDAPVGRIMNAGFPRMSVNDPIAWLGERLA
                     VETAVAIEEGDEFLGLVTRADYLTRIRNEKAKA"
     gene            1101..2264
                     /locus_tag="VFX38_04590"
     CDS             1101..2264
                     /locus_tag="VFX38_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017846419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX5314174.1"
                     /translation="MSETELRFETLCVHGGQAPDPVTGAVMPPISQASTFAQASPGKH
                     RGYEYSRTRNPTRLAWERAIAALEGGCEAFAFASGMAAAATALELLPAGSEVLAMDDL
                     YGGTHRLFQKVRAESAGLRFVYADLSDTKAIASSFGADTRMVWIETPSNPMLKLVDLA
                     AVAEAAHRVGALAVCDNTFATPWCQRPLEFGFDIVLHSATKYLNGHSDMVGGVLVAGT
                     PELAARIAFLQNSIGAVAGPFDAYLALRGVKTLALRMERHSASALAIAEHLEGHPKVR
                     RVIYPGLSSHPQHTLAKHQMQRGFSGMLTAILATDLAGARRFLERVELFTLAESLGGV
                     ESLIEHPALMTHAAMSEAQRRALGFDDGLVRLSVGIEDPRDLIADLDAALAAV"
     gene            2327..2845
                     /locus_tag="VFX38_04595"
     CDS             2327..2845
                     /locus_tag="VFX38_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HEX5314175.1"
                     /translation="MSEDEQVINKPPPMFDPNAELEYLLAGAKTGKVSIVTVYQALLR
                     APVYALFDREMTPENLDPAGKALIFETSDMGNLMVLFTAPELSEKLGSDAGEFTHPAQ
                     LSGEYLVNVLSENTGVILNPGHDYGMKLAAKGLQQLKRDFGSHGGPQQTGAGGGAQGI
                     PGPSGGAFPTLN"
     gene            complement(2916..4103)
                     /locus_tag="VFX38_04600"
     CDS             complement(2916..4103)
                     /locus_tag="VFX38_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020216921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX5314176.1"
                     /translation="MNARDGAQERVTGAQEELFALSRRIHSHPELGWQEERAVGWLTD
                     MLADAGFSVEKGVCDLPTAFLARAGSGPLHVGICAEYDSLPEIGHACGHNLIAAMAVG
                     AAMAAARVADEVGLTVSIIGTPAEEVGNAGGKILMLERGAFDGVHAAMMAHPAPLDSV
                     TPKLIAASMFEAHYTGKEAHASAFPELGINAADALTVAQTAIGLLRQHIRASDRIHGI
                     ITRGGDAPNVVPAHTAAKYIFRSETLEELDALRPKVHRCFEAGALATGSKLEIVGGDR
                     PYAHVLHDADMAACYRRNAETLGRRFPGPEALPERASGSTDMGNISLTLPAIHPFIGI
                     NSLPAVNHQPEFTAHCIGADAERAILDGALAMAWTAIDMATDEELRRRLIRQGAQRRG
                     AQK"
     gene            complement(4203..>4439)
                     /locus_tag="VFX38_04605"
     CDS             complement(4203..>4439)
                     /locus_tag="VFX38_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314177.1"
                     /translation="LRGGGSSRQLQTIAWFVEAWFDRWLNDDLSANQRLLACTVNGES
                     TAGLLSDNFLSGVYLPDASVDTSDYKSYLGQHCP"
BASE COUNT          758 a         1452 c         1452 g          777 t
ORIGIN      
        1 ctgatcgtga tgccggacaa gatgagccgc gagaaggtgt tcaacgtgca ggcgatgggc
       61 gccgaagtcg tgcttacgcg ctcggacgtg cgcaagggcc atcccgagta ctaccaggat
      121 ctcgcccaac gtctcgccgg cgagcgcggc ggctactaca tcaaccagtt cgcgaacgtc
      181 gacaatcccg cggcgcacga ggcgacgacc ggccccgaaa ttttcgaaca gatggacggg
      241 cgggtggacg cgatcgtgct cggcgtcggc accagcggca cgctcaccgg cgtttcgcgg
      301 tatttcgcga aggcttcgcc cgcgaccgaa ttcgtgctcg ccgatccgga aggctcggtg
      361 cttgccgatt acatcgacac gggaaaaatc ggcgaagcgc gccgttggct ggtcgagggc
      421 atcggcgagg acttcattcc gacgatcgcc gatctttcgg cggtgaagcg cgcctacacg
      481 atttcggatg ccgaatcctt cgcgaccgcg cgcgaagtgt tgcgcaagga aggaatcctg
      541 ctcggctcct cgtcgggcac cctgatcgcg gcggctctgc gctactgccg cgagcagacc
      601 gaagcgaagc gcgtgctcac cctcggctgc gataccggcg ccaagtatct ctccaagttc
      661 tacaacgatt tctggctcgc cgagcgcggc tttctggtgc ccgagagcgc gggcaacctg
      721 agtgatctca tcacccgccc gcatcgccat cacgcgaccg tgaccgttgc gccggaggat
      781 acgctcgcaa cggcgcacga ccggctgcgc gacggcggct attcgcagct tccggtcgtc
      841 cgtgagggac gtctcgtggg ggcgatcacc gagggcgatc tgctgcacgc cgtgatcgat
      901 ctcgcgctgc ccttcgacgc gcccgtggga cgcatcatga acgccggctt tccgcgcatg
      961 agcgtgaacg acccgatcgc ctggctcggc gagcggctcg ccgtggagac cgccgtcgcg
     1021 atcgaggaag gcgacgagtt tctcggcctc gtcacgcgcg ccgactacct cacccgcatc
     1081 cgcaacgaaa aggcgaaagc atgagcgaga cggaactgcg cttcgagacg ctctgcgtcc
     1141 acggcggaca ggctcccgat ccggtgaccg gagcggtgat gccgccgatt tcccaggcct
     1201 cgaccttcgc gcaggcgagc ccggggaaac atcgcggcta cgagtattcg cgcacccgca
     1261 atccgacccg cctcgcctgg gagcgggcga tcgccgcgct cgaaggcggc tgcgaggcgt
     1321 tcgcattcgc ctcgggcatg gcggcggcgg cgaccgcgct cgaactcctt cccgcgggaa
     1381 gcgaagtgct cgccatggac gatctctatg gcggcacgca tcggctgttc cagaaggtgc
     1441 gcgccgaaag tgcggggctg cgtttcgtct atgcggacct ttccgatacc aaggcgatcg
     1501 catcttcctt cggtgcggac acgcgcatgg tctggatcga aactccgagc aatccgatgc
     1561 tcaagctcgt ggatctcgcg gccgtcgccg aggccgcgca ccgggtcggc gcgctcgccg
     1621 tttgtgacaa caccttcgcg actccctggt gtcagcgccc gctcgaattc ggcttcgata
     1681 tcgtgctcca ttcggcgacc aagtatctca acggacactc ggacatggtc ggcggcgtgc
     1741 tcgtcgccgg cacgcccgaa ctcgccgcgc gcatcgcctt tttgcagaac tcgatcggtg
     1801 ccgtggcggg cccgttcgat gcctatcttg cgcttcgcgg agtgaagacg ctcgcgctgc
     1861 gcatggagcg gcattcggcg agtgcgctcg cgatcgccga acatctcgaa ggccatccga
     1921 aagttcgccg cgtgatttat ccgggacttt cgagccatcc gcaacacacg ctcgcgaagc
     1981 atcagatgca acgcggcttc agcggcatgc tgaccgcgat tctcgcgacc gatcttgccg
     2041 gagcgcgccg ctttctcgaa cgcgtcgagc ttttcacgct cgcggaaagt ctcggcggcg
     2101 tcgagagcct gatcgaacat ccggcgctca tgacgcatgc ggcaatgagc gaagcgcagc
     2161 ggcgcgcact cggattcgac gatggcctgg tgcggctttc ggtcggtatc gaagatccgc
     2221 gcgatctaat cgccgatctc gacgccgcgc tcgctgccgt ctagcgagcg gcaagagccg
     2281 gttcgccggc ctcacctcca acgcaatcgc aacaggaacc acgtacatga gcgaagacga
     2341 acaggtcatc aacaagccgc cgccgatgtt cgaccccaat gcggaactcg aatatcttct
     2401 cgccggtgcc aagacgggga aagtctccat cgtcaccgtg tatcaggcgc tcctgcgggc
     2461 gccggtctat gcgctgttcg accgggagat gacgcccgag aatctcgatc ccgccggcaa
     2521 ggcgctgatc ttcgagactt ccgacatggg caacctgatg gtgctcttca ccgcgccgga
     2581 actctccgag aaactcggct cggatgccgg cgagtttacc catcccgcgc agctctccgg
     2641 cgaatatctt gtcaacgtgc tctccgagaa caccggcgtc atcctgaatc ccggacacga
     2701 ctacggcatg aaacttgccg ccaagggcct gcaacaactg aagcgggact tcggcagtca
     2761 tggcggcccg cagcagacgg gcgcaggcgg cggcgcgcag gggatacccg gaccgtccgg
     2821 gggcgccttc ccgaccttga attagcgcgc ggaagccggc gcgattcccg ggcggtcacg
     2881 cgcccgcggg cagccggaac cctcggcggt ggggattatt tctgcgcgcc gcgtcgttgc
     2941 gcaccctgcc ggatcaggcg gcgacgaagt tcttcgtccg tcgccatgtc gatcgcggtc
     3001 caggccatcg cgagcgcgcc gtcgagaatc gcgcgctcgg catccgctcc gatgcagtga
     3061 gcggtgaact cgggctgatg attcacggcc ggcagggaat tgatgccgat gaagggatga
     3121 atcgcaggca gcgtgagcga gatgttgccc atgtccgtcg agccggaggc acgttcggga
     3181 agggcttcgg gaccgggaaa ccgacgcccc agcgtctccg cgttgcgccg gtagcaggcc
     3241 gccatgtccg cgtcgtgcag gacgtgcgcg tagggcctgt cgccgcccac aatctcgagc
     3301 ttcgatccgg tcgcgagcgc ccccgcctcg aaacagcgat gaaccttcgg gcgcagtgcg
     3361 tcgagttcct ccagcgtttc cgagcggaag atgtatttcg cggcggtgtg cgctggcacg
     3421 acgttggggg cgtcgccacc tcgggtaatg attccatgga tgcggtcgct ggcgcgaatg
     3481 tgctggcgca gcaggccgat cgcggtttgt gcgaccgtca gggcgtcggc ggcattgatg
     3541 ccgagttcgg gaaaggccga ggcgtgcgct tccttgccgg tgtagtgcgc ttcgaacatg
     3601 gaggccgcga tcagtttcgg cgtgaccgaa tcgagcgggg cgggatgcgc catcatggcc
     3661 gcgtgcactc cgtcgaaggc gccgcgctcg agcatgagaa tcttgccgcc ggcatttccg
     3721 acttcttcgg cgggcgttcc gatgatgctg accgtcaagc cgacttcgtc ggcgacccgc
     3781 gccgctgcca ttgcggcgcc gacggccatt gcggcgatga ggttgtggcc gcaggcatga
     3841 ccgatttcgg gaaggctgtc gtattcggcg cagatgccga catgcagcgg gccgcttcca
     3901 gcgcgtgcga gaaatgcggt cgggagatcg cagactccct tttccaccga gaagccggcg
     3961 tcggcgagca tgtcggtgag ccagccgacc gcacgttcct cctgccagcc gagttccgga
     4021 tgggaatgga tgcgccggct gagcgcaaac agctcctctt gcgcgcctgt gacccgttcc
     4081 tgtgcaccgt ccctggcatt catgtgggct cccgcggccg ggttgggagg cgaggtcttc
     4141 ccgataccat cgcccgagcc tatcctaaac ccgggagcca gtgccggccg atccgcactt
     4201 cttcaagggc aatgttgtcc gaggtagctc ttgtaatcgg aggtgtcgac ggaggcatcg
     4261 ggcaagtaca cgccggaaag aaaattgtcg ctcaggagcc ccgcggtgct ctcgccgttg
     4321 acggtgcagg caagcaaacg ctgattggcg ctgagatcgt cgttcagcca gcgatcgaac
     4381 catgcctcga cgaaccaggc gatggtctgc aactgcctgc tgctgcctcc gcccctgag
//
LOCUS       DASUJG010000216         2466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1043715, whole genome shotgun sequence.
ACCESSION   DASUJG010000216 DASUJG010000000
VERSION     DASUJG010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043715"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="VFX38_04610"
     CDS             <1..748
                     /locus_tag="VFX38_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEX5314178.1"
                     /translation="SESEIWRSHRGCARVQDPYSTRCQPQVLGAAWDTFAHASAVLAR
                     EVNAATDNPLVFGDSAISGGNFHAAPVGLIADFLAIALADVASLSERRVDCFMRRVNP
                     ALPAFLAHRPGLESGFMLAQVSAAALASENKTLAHPASVDSVPTSAGQEDHVSMAPWA
                     GLKLLRIAENLRTILAIELACAAAALDHRRPLRTTPELEPVYKAIRSIVPAWTGDRRH
                     DRDIAALAGLLETGRIAEALPQAAWRSPWA"
     gene            815..1915
                     /locus_tag="VFX38_04615"
     CDS             815..1915
                     /locus_tag="VFX38_04615"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5314179.1"
                     /translation="MSPQENRSAAAWRGLVAVAAVIVVIAGMRAAADLIVPFLLALSI
                     AIICLPPLAWLRRRNVPVSLSVTIVIVLIVMAIGGFGTLIANSIHSIIGDLPDYQQRF
                     QGVLDKAMQFAHRMGWQGSLHSVFEQINPKNATPVLTQFFKGFGTLLGESILIVLTVI
                     FLLFEASVLPGKLRTMPGDRHGHRSLEVFRSFVDSIQRYVFLKTIVSIVLGVLVALVL
                     WGFGVRYPVLWGVLAFLLNFVPNVGAFLSSIPPILLALVQGGWPLAAYAGSTLFVIHF
                     LTGNLVEPIIMGERLGLSVLVVWLSLIVWGWVLGPVGMLLSVPLTMILKIGFEAYPET
                     RWVAVLLGPAPSSGNRLFEWLRPWRKRSARKQ"
     gene            complement(1956..>2466)
                     /locus_tag="VFX38_04620"
     CDS             complement(1956..>2466)
                     /locus_tag="VFX38_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5314180.1"
                     /translation="GIWGKAHQNQMLAATALFAVGGALWAGNDNRFGHTLWQSSDALM
                     LSAASVFALNNVFQRERPRDTADPDRWFQGFGNTSFPSGHVTLTAAAITPLVLEYGRE
                     HPWVYALEVIPLYEAVGRVKTQEHWQSDVLAGWAIGSLIGWYAHSRKVPLLVMILPRG
                     FAVGLQARF"
BASE COUNT          379 a          849 c          760 g          478 t
ORIGIN      
        1 cagcgagagc gaaatctggc gctcgcaccg cggctgcgct cgcgtgcagg atccctactc
       61 gacgcgctgt cagccgcagg tgctgggagc ggcctgggat accttcgccc acgcctcggc
      121 cgtgctggcg cgcgaagtca atgcggcgac cgacaatcca ctggtcttcg gcgacagcgc
      181 aatttcgggc ggcaatttcc atgctgcacc ggtgggcctc atcgcggatt ttctggcgat
      241 cgccctcgcc gatgtcgcga gcctctcgga gcggcgggtg gactgcttca tgcgccgcgt
      301 gaatccagcg cttcccgcgt ttctcgcgca tcgcccggga ctcgaatcgg gcttcatgct
      361 cgcgcaggtg agcgccgccg ccctcgcctc ggaaaacaag accctcgccc atccggcgag
      421 cgtggacagc gtgccgacct cggcgggaca ggaagatcac gtaagcatgg cgccctgggc
      481 ggggctgaag cttctgcgca tcgccgagaa tctgcgcacg attctcgcga tcgaactcgc
      541 gtgcgccgcc gccgcgctcg atcatcgacg gccgctccga acgactcccg aactcgagcc
      601 cgtgtacaaa gcgatccgaa gcatcgttcc cgcatggacg ggcgaccgcc gccacgaccg
      661 cgacatcgcc gctctcgccg gacttctcga aacggggcgg atcgccgagg cgttgccgca
      721 ggcggcctgg cggtcaccct gggcctgaag cgttcgccac cggggcggca ctcgtgtacc
      781 atcgcccgga cctgaagcag gaactggcgc gaacgtgagc ccgcaggaaa atcgcagcgc
      841 cgctgcctgg cggggactcg tcgccgttgc cgccgtgatc gtcgtcatcg ccggcatgcg
      901 cgcggcggcc gatctcatcg tcccttttct gttggcgctt tcgatcgcga tcatctgtct
      961 tccgccgctt gcctggttgc gacggcgcaa cgtccccgtt tcgctctcgg tcaccatcgt
     1021 gatcgtgctc atcgtcatgg cgatcggcgg cttcggcacc ctcatcgcga attccattca
     1081 cagcatcatc ggcgatctgc ccgactatca gcagcgcttc cagggcgtgc tggacaaggc
     1141 catgcagttc gcccatcgca tgggctggca gggatcgctg cacagcgtct tcgagcagat
     1201 caatccgaag aacgcgacgc cggtgctcac gcaatttttc aagggtttcg gcacgctgct
     1261 gggcgaaagc attctcatcg tgctcacggt gatttttttg ctcttcgagg cgagcgtgct
     1321 tcccggcaag cttcgcacca tgcccggcga tcgtcatggt catcgttcgc tcgaagtgtt
     1381 ccgctccttc gtcgacagca ttcagcgcta cgtgtttctc aagaccatcg tcagcatcgt
     1441 gctcggcgtt ctcgtcgcgc tggttctctg gggattcggc gtgcgctacc cggttctctg
     1501 gggagtgctc gcgtttctgc tcaacttcgt gcccaacgtc ggcgccttcc tttcttccat
     1561 tcctccgatc ctgctcgcac tcgtccaggg cggctggccg ctcgcggctt acgccggctc
     1621 gacgctcttt gtcattcact ttctcaccgg caacctcgtc gagccgatca tcatgggcga
     1681 gcgcctcggg ctctctgtgc tggtggtgtg gctttcgctg atcgtctggg gctgggtgct
     1741 cgggcccgtc gggatgctgc tctcggtgcc cctcaccatg atcctcaaga tcggtttcga
     1801 ggcctacccc gaaacgcgct gggtcgccgt tctcctcggc cccgcgccga gcagcgggaa
     1861 tcgcctcttc gaatggctgc ggccctggcg caagcgctcg gcgcgaaagc aatagccgat
     1921 cgtcgccacg cattgcgccg catgacattt cgagtctaga agcgcgcctg cagcccgacg
     1981 gcaaagcctc gcggcagaat catcaccaga agcggcacct tgcgggaatg ggcataccag
     2041 ccgatcaggc tgccgatggc ccagcccgcg agcacgtcgc tctgccagtg ttcctgcgtc
     2101 ttgacgcgac ccacggcctc gtacagcgga atgacttcca gcgcgtagac ccacggatgc
     2161 tcgcgcccgt actcgagcac gagcggcgta atggcggcag ccgtgagcgt cacgtgaccg
     2221 ctcggaaagc tggtgttgcc gaatccctgg aaccagcgat ccggatcggc agtatcgcgc
     2281 gggcgctcgc gctgaaacac attgttgagg gcgaacacgc tcgcggccga cagcatcaat
     2341 gcatcgctcg attgccacag cgtgtggccg aagcggttgt cgttgcccgc ccagagcgcg
     2401 ccgccgacgg cgaagagcgc ggtcgccgcc agcatctgat tctgatgcgc cttgccccag
     2461 atgccg
//
LOCUS       DASUJG010000217         2075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1287708, whole genome shotgun sequence.
ACCESSION   DASUJG010000217 DASUJG010000000
VERSION     DASUJG010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287708"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VFX38_04625"
     CDS             <1..972
                     /locus_tag="VFX38_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018002625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5314181.1"
                     /translation="AGEIVFVRGATEAINLVAGSWGVANVGHGDEILLTELEHHSNIV
                     PWQLLAERVGARIVVAPLAADGSIPIEGFRERLSARTRIAAIAHVSNALGTVNPVAEM
                     TALAHAAGAKVLVDGAQAAGHLPLDVAALGCDFYALSAHKMYGPMGIGALYLPEEIAA
                     GMPPWQGGGDMIRAVRFEETLYADPPQRFEAGTPNVAGAIGLAAAIEYLRTLAMPAVA
                     AHEAELLAHAVECLRALRGLRLIGQPPQRAGVISFTLEGVHPHDLGTLLDHAGIAIRT
                     GHHCAMPVMEHYGLAATARASFGVYNDEEDVERLVAALAEAREFFDT"
     gene            972..1424
                     /locus_tag="VFX38_04630"
     CDS             972..1424
                     /locus_tag="VFX38_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014148483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEX5314182.1"
                     /translation="MDADLAALYRDLIIDHNRSPRNFHALDPPCRSAEGYNPLCGDRL
                     TVYLRLDGDRIEEAAFQGSGCAISTASASLMTESLRGRTPAEARELFRDVRALLTGGD
                     DAPAADELGKLGALAGVRAYPSRVKCATLAWHTLQSALAGDDERVTTE"
     gene            1430..1978
                     /gene="sufT"
                     /locus_tag="VFX38_04635"
     CDS             1430..1978
                     /gene="sufT"
                     /locus_tag="VFX38_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HEX5314183.1"
                     /translation="MWGKQAITVSRDCPGVLVPAGTEVVIPEGTDVQITQSLGGSVTV
                     YIGGNLVRVDAKDAGALGLEVSDPAASMPADPSDEEFEAIVQDQLKTCFDPEIPINIV
                     DLGLIYKCEIGKREDGKRDISIDLSLTAPGCGMGDILLDDIRAKLALIPTCGAVNAEL
                     VFDPPWGPAMMSDEAKLQTGMY"
BASE COUNT          362 a          689 c          680 g          344 t
ORIGIN      
        1 gcgggcgaga tcgtcttcgt gcgcggcgca accgaggcca tcaatctcgt cgccggcagt
       61 tggggtgtcg cgaacgtcgg ccacggcgac gaaattcttc tgaccgaact ggagcaccac
      121 tccaacatcg tgccctggca gcttctggcc gagcgtgtcg gcgcgagaat cgtggttgca
      181 ccgctcgccg ccgacggcag cattcccatc gaagggtttc gcgaacggct gtcggcgcgc
      241 acgcgcatcg ccgcgatcgc ccacgtctcc aatgcgctcg gcacggtgaa tccggtggcg
      301 gaaatgaccg cgctcgcgca tgccgcgggc gcaaaggttc ttgtcgacgg tgcgcaggcc
      361 gccgggcatc tgccgctcga cgtggccgct ctcggctgcg acttttatgc gctgtcggcg
      421 cacaagatgt acgggccgat gggcatcgga gccctgtatc tccccgagga gatcgcggcc
      481 gggatgccgc cctggcaagg cggcggtgac atgattcgcg cggtgcgctt cgaggaaacg
      541 ctgtacgccg atccgccgca gcgcttcgag gccggcacgc cgaatgtcgc gggcgccatc
      601 gggctggcgg cggccatcga gtatctgcgg actctcgcga tgccggccgt ggcggcgcac
      661 gaagccgaac tgctcgccca cgccgtcgag tgcctgcgcg cgctgcgcgg tctgcgcctg
      721 atcggccagc cgccgcaacg cgccggagtc atttccttca cgctcgaggg cgtgcatccg
      781 cacgatctgg ggacgctgct cgatcacgcc ggcatcgcga tccgcaccgg ccatcactgc
      841 gcgatgccgg tgatggagca ttacggtctc gcggccacgg cgcgcgcatc cttcggggtt
      901 tacaacgacg aggaagacgt cgagcggttg gtagcggcgc tcgcggaggc gcgggagttt
      961 ttcgacacat gatggacgcc gatctcgcgg ctctctatcg cgatctcatc atcgatcaca
     1021 accgcagtcc gcgcaatttt cacgcgctcg atcctccctg ccgcagcgcc gagggctaca
     1081 accctctttg cggcgatcgt ctcaccgtgt atctgcgcct cgacggcgat cgcatcgaag
     1141 aagccgcctt ccagggttcc ggctgcgcga tctcgacggc ctccgcctcg ctcatgacgg
     1201 aaagtctccg cggcaggact ccggccgagg cgcgggagct tttccgcgac gtgcgcgcgc
     1261 ttctcaccgg cggcgatgac gcgcccgcgg ccgatgagct gggaaaactc ggcgcgctcg
     1321 ccggagtgcg ggcctatccc tcgcgggtga aatgcgcgac gctcgcctgg catacgcttc
     1381 aatccgcact cgccggagac gacgagcgcg tgacgacgga gtaagcccga tgtggggaaa
     1441 acaagcgatc accgtttccc gcgactgccc cggcgtactc gttccggccg gtaccgaagt
     1501 agtcattccc gaagggaccg acgtgcagat cacccaatcc ctcggcggca gcgtcaccgt
     1561 ctacatcggc ggcaatctcg tgcgcgtgga cgcgaaagac gccggcgcgc tcggcctcga
     1621 ggtgagcgat ccggcggctt cgatgcccgc cgatccgagc gacgaggaat tcgaagcgat
     1681 cgtgcaggat cagctcaaga cctgcttcga cccggaaatc ccgatcaaca tcgtggacct
     1741 cggactcatc tacaagtgcg agatcggcaa gcgcgaggac ggcaaacgcg acatctcgat
     1801 cgatctttct cttaccgcgc ccggctgcgg catgggcgac atccttctcg atgacatccg
     1861 tgcgaagctc gcattgatcc ccacttgcgg cgccgtcaac gcggaactcg tgttcgatcc
     1921 gccctggggg cccgcgatga tgtcggacga ggccaagctc cagaccggaa tgtactgaag
     1981 aagaagcgag ggggaaaaat gacgagtgac ccagaaaacc ggaaggaaga agccgtcgtg
     2041 aacgattggc tggacgtatg cgcgatcggc gagat
//
LOCUS       DASUJG010000218         2334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1655807, whole genome shotgun sequence.
ACCESSION   DASUJG010000218 DASUJG010000000
VERSION     DASUJG010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655807"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1481
                     /locus_tag="VFX38_04640"
     CDS             <1..1481
                     /locus_tag="VFX38_04640"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475792.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. Subunit beta'
                     binds to sigma factor allowing it to bind to the -10
                     region of the promoter; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX5314184.1"
                     /translation="PGTQLTMRTFHIGGAASRSAAISHIQVKAGGTVHLHNFKHVENA
                     QGHLVAISRSGELAVVDEAGRERERYRVPYGAAVTVRDGAGVEGGQIVANWDPHTHPI
                     VTEVAGRLRLEDFAEGMAVRQEVDEVTGLVSIKVLDPKQRPAAGRELRPVLRVVNDKG
                     EPLTLGASDIPAQYALPGGALVTLADGAAVEVGDVIARIPQETSKTRDITGGLPRVAD
                     LFEARRPKEPAILAEASGTVSYGKDTKGKQRLLITTDAGEQVEMLLPKWRQINVFEGE
                     HVSRGEIIADGEPDPHDIVRVLGVHRLAEFLVREIQDVYRLQGVKINDKHIEVIIRQM
                     LRKGEITESGDTGFLKGEQVDWARIIEANEQAASDKAKPAEHVPMLLGITKASLATES
                     FISAASFQETTRVLTEAAVRGAEDQLRGLKENVIVGRLIPAGTGYAYHRERRRGSPEE
                     EARAEAERLFAGSGGEEGEESSGESTPEITPTAEMPVESEKT"
     gene            1564..1938
                     /gene="rpsL"
                     /locus_tag="VFX38_04645"
     CDS             1564..1938
                     /gene="rpsL"
                     /locus_tag="VFX38_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006083605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX5314185.1"
                     /translation="MATTNQLVRRARRAPREKNRVPALAGSPQKRGVCTRVYTTTPKK
                     PNSALRKVARVRLTNGFEVTSYIGGQGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGAL
                     DCAGVGDRRQGRSKYGAKRPKS"
     gene            1958..>2334
                     /gene="rpsG"
                     /locus_tag="VFX38_04650"
     CDS             1958..>2334
                     /gene="rpsG"
                     /locus_tag="VFX38_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX5314186.1"
                     /translation="MPRRREIPKRQILPDPKYGSEQLAKFINVVMTRGKKSVAERIVY
                     GALDKVKERQNEPLSVVEQAFENTRPLVEVKSRRVGGATYQVPVEVRPARQDALAMRW
                     VIEAARARSEKSMALRLAGELMDA"
BASE COUNT          455 a          718 c          813 g          348 t
ORIGIN      
        1 agccgggcac gcagctcacg atgcggacct tccacatcgg cggcgcggcg tcgcgttcgg
       61 ccgcgatcag ccacatccag gtgaaggccg ggggcacggt acatcttcac aatttcaaac
      121 atgtggaaaa cgcccagggt catcttgtcg cgatctcgcg ctcgggagag ctggccgtgg
      181 tggacgaggc ggggcgcgag cgggaacgct atcgcgtgcc gtacggcgcc gcagtgacgg
      241 tgcgcgacgg cgccggagtc gagggcgggc agatcgtcgc caactgggat ccgcacaccc
      301 atccgatcgt gaccgaggtc gccggacgcc tgcgtctgga agacttcgcc gagggaatgg
      361 cggtgcgcca ggaggtggat gaggtcacgg ggctcgtgag catcaaggtg ctcgatccca
      421 agcaacgacc ggccgccgga cgggaattgc gcccggttct tcgggtggtc aacgacaagg
      481 gcgagccact cactctcggt gcgagcgaca tccccgcgca atatgcgctt cccggcggcg
      541 ccctggtcac ccttgccgac ggcgcggcgg tcgaggtcgg agacgtgatc gcgcggattc
      601 cgcaagagac ctccaagact cgcgacatta ccggcggtct gccgcgcgtg gccgacctgt
      661 tcgaggcccg caggccgaag gagccggcga ttctcgccga ggcgagcggg accgtttcct
      721 acggcaagga caccaagggg aaacagcgcc tgctgatcac gaccgacgcg ggcgagcaag
      781 tcgagatgtt gttgcccaag tggcggcaaa tcaatgtctt cgagggcgag catgtcagcc
      841 gcggcgaaat catcgccgac ggcgaacccg atccgcacga catcgtgcgg gtgctcggcg
      901 tgcaccggct ggcggagttt ctggtgcggg aaatccagga cgtttaccgt ctgcagggcg
      961 tgaagatcaa cgacaagcac atcgaagtca tcatccgcca gatgctgcgc aagggcgaga
     1021 tcaccgaatc cggcgacacc ggctttctca agggagagca ggtggactgg gcacgcatca
     1081 tcgaggcgaa cgagcaggct gcgagcgaca aggcgaagcc ggcagagcat gttccgatgc
     1141 tgctcggcat caccaaggct tcgctcgcga ccgaatcgtt catctctgca gcttcgttcc
     1201 aggagacgac gcgggtgttg accgaggcgg ccgtgcgggg tgcggaggat cagctgcgcg
     1261 gcctgaagga gaacgtcatc gtcgggcggc tgataccggc gggcaccggc tatgcctatc
     1321 atcgggagcg ccgccgcgga tcgcccgagg aggaggcccg cgccgaagcg gaacgcctgt
     1381 ttgccggctc cgggggcgag gaaggagaag agtcatccgg cgaaagtacg ccggagataa
     1441 cgccgaccgc ggaaatgccg gtggagtccg agaaaacttg acacacaggc cgcagctctt
     1501 tagtatttac ccgtttagcc ggggccggca ttgccggccc cgcttatttc taccgagagg
     1561 gtgatggcga caaccaatca gctcgttcgc cgggcgcgac gcgccccgcg ggagaagaac
     1621 agggtgccgg cactggccgg cagtccgcag aagcgcggcg tatgcacgcg cgtctacacg
     1681 actacaccca agaagcccaa ctcggcgctg cgcaaagtcg cgcgcgtgcg tttgaccaac
     1741 ggcttcgagg tgacgagcta tatcggcggc caagggcata accttcagga acactcggtg
     1801 gtgctgattc gcggtggacg cgtcaaggat cttcccggcg ttcgttatca caccgttcgc
     1861 ggcgctctcg attgcgccgg ggtcggcgat cgccgccagg gacgttccaa atacggcgcc
     1921 aagcggccca agagctgagt ggcagcgcgg agaacccatg cccagacgac gtgaaatacc
     1981 gaagcgacag atcctgcccg accccaagta cgggagcgag caactcgcga agttcatcaa
     2041 cgtggtcatg actcgaggca agaaatcggt cgccgaacgc atcgtctacg gggcgctcga
     2101 caaggtgaag gagcggcaga acgaaccgct ctcggtggtc gagcaggcct tcgagaacac
     2161 ccgcccgctg gtcgaggtca agtcgcgtcg cgtcggcggc gccacctatc aggtgcccgt
     2221 cgaggtacgc ccggcgcggc aggatgcgct cgccatgcgc tgggtcatcg aggcggctcg
     2281 cgcccgctcg gagaagtcga tggcgttgcg gctcgccggc gagttgatgg atgc
//
LOCUS       DASUJG010000219         2093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1533296, whole genome shotgun sequence.
ACCESSION   DASUJG010000219 DASUJG010000000
VERSION     DASUJG010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533296"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..661
                     /locus_tag="VFX38_04655"
     CDS             11..661
                     /locus_tag="VFX38_04655"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX5314187.1"
                     /translation="MNLILLGPPGAGKGTQADLLKKHRGLVQLSTGDMLRAAVASGSD
                     LGKRVKSIMEAGQLVPDETMIQMIEARIGEPDCGKGFILDGFPRTTRQAEALEAMLVR
                     KGTRLDRVVELKVNGAELVKRIAGRYACAKCGAGYHDTFKTPKRPGICDICGSTEFVR
                     RKDDRPETVAARLEAYHAQTSQLSAYYAAKGLLSEVDGMADIPQVFRQIEAALDKS"
     gene            760..1134
                     /gene="rpsM"
                     /locus_tag="VFX38_04660"
     CDS             760..1134
                     /gene="rpsM"
                     /locus_tag="VFX38_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX5314188.1"
                     /translation="MARIAGVNIPTEKRVEVALTYIYGIGPHRAQEICHKVGIPAERR
                     VNQLTEDEVIRIREAIDRDYHVEGDLRREVAMNIKRLMDLGTYRGLRHRKGLPVRGQR
                     THTNARTRKGPAKPIAGKKKAV"
     gene            1153..1557
                     /gene="rpsK"
                     /locus_tag="VFX38_04665"
     CDS             1153..1557
                     /gene="rpsK"
                     /locus_tag="VFX38_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX5314189.1"
                     /translation="MVKAAETSAAPRPRRRERKNITSGVAHVNATFNNTVITITDAQG
                     NTIAWSSSGSKGFKGSRKSTPYAAQVAAEDAGKKAMEHGMRTLEVQVKGPGSGRESAL
                     RMLQAVGFTITSIRDVTPVPHNGCRPPKRRRV"
     gene            1661..>2093
                     /locus_tag="VFX38_04670"
     CDS             1661..>2093
                     /locus_tag="VFX38_04670"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240055.1"
                     /note="catalyzes the transcription of DNA into RNA using
                     the four ribonucleoside triphosphates as substrates.
                     Dimerization of the alpha subunit is the first step in the
                     sequential assembly of subunits to form the holoenzyme;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX5314190.1"
                     /translation="MLVIQKNWTELIKPSKLDIQASNEPARRATIVAEPLERGFGLTL
                     GNALRRVLLSSLQGAAVTAVQIEGILHEFSSIPGVREDVTDIVLNIKSLAIRLHGDGP
                     KRVSLKATGPGEVRAGQIETGHDIEIMNKDLVICTLDQSAKI"
BASE COUNT          415 a          679 c          662 g          337 t
ORIGIN      
        1 gtcgcaacgg atgaacctca tccttctcgg gccgccgggt gcgggcaagg ggacgcaagc
       61 ggatctcctc aagaagcacc gcggactcgt gcagctttcg accggcgaca tgctacgcgc
      121 cgcggtggct tccggctccg acctcggcaa gcgggtgaaa tcgatcatgg aagccggcca
      181 gctcgtgccg gacgagacca tgatccagat gatcgaggcg cggatcgggg agcccgattg
      241 cggcaagggc ttcatcctcg acggcttccc gcgcaccacc cgccaggctg aggcgctgga
      301 ggcgatgctg gtccgcaagg gaacgcggct cgaccgtgtg gtcgagctca aggtgaacgg
      361 cgccgagctc gtcaagcgca tcgccgggcg ctacgcctgc gccaaatgcg gcgccggcta
      421 tcatgacacc ttcaagacac cgaaaagacc cggaatttgc gacatatgcg gctcgacgga
      481 gtttgtgcgc cggaaggatg atcgcccgga aacggtcgcc gcccggctcg aggcctacca
      541 cgcccaaacg tcgcagctat cggcctatta cgcggccaaa gggctgcttt ccgaggtcga
      601 cgggatggcc gatattcccc aggttttccg ccagattgag gcggcgcttg acaagtcgta
      661 aaaggttgac tgaggacggt tttttcctat aatcccggcc tctttcggac ccgggcgaat
      721 ttccccgggt ttttcaacga tcaaacggga gtctttccct tggcgcgcat cgctggcgta
      781 aacatcccta ctgagaagcg ggtggaggtc gccctcacct acatctacgg aatcggcccc
      841 catcgcgcgc aggaaatttg ccataaagtt ggcattcccg ccgagcggcg cgtcaaccaa
      901 cttaccgaag acgaagttat ccgcatccgc gaggcgatcg atcgcgacta tcatgtcgaa
      961 ggcgatctgc ggcgcgaagt cgcgatgaac attaagcgcc tcatggatct cggcacgtac
     1021 cgcgggcttc gtcaccgcaa gggcttgccc gtgcgcggtc agcgcaccca caccaacgcg
     1081 cgcacccgca aagggcccgc gaagccgatc gccggcaaga agaaagccgt ctaagtccga
     1141 ggacaagaag ttatggtcaa ggccgccgaa acgtccgccg cgccgcgccc ccgtcgccgc
     1201 gagcgcaaga acatcacatc gggcgttgcg catgtgaacg ccacgttcaa caacacggtg
     1261 atcaccatca ccgacgcgca gggcaacacc atcgcgtggt cgtcttcggg ctcgaaggga
     1321 ttcaagggtt cgcgcaaatc gacgccctac gccgcgcagg tcgcggccga ggacgccggc
     1381 aagaaggcga tggagcacgg catgcgtacc ctcgaagtcc aggtgaaggg tcccggctcg
     1441 ggccgcgagt ccgcgcttcg catgctgcag gcggtgggct tcaccatcac gtcgattcgc
     1501 gacgtgacgc cggtgcccca caacggctgc cggccgccga agcggcgccg cgtttgattc
     1561 agtgcgtttc ggcgaagccc cgggcttcgc tgcttttgat cgggcgggat agccgctcac
     1621 ttggagatgc ggacccgagg tccgcgatcg gaacgaggac atgctcgtga ttcagaagaa
     1681 ttggacggaa ttgatcaaac cgagcaagct cgacatccag gccagcaacg agcctgcccg
     1741 ccgcgcgacc atcgtcgccg agccgctgga acgcggtttc gggctcacgc tcggcaacgc
     1801 gctccggcgt gtgctcttgt cgtcgctcca gggtgccgcg gtgacggcgg tccagatcga
     1861 gggaatcctg cacgaattct cctcgatccc cggcgtccgc gaggacgtga ccgacatcgt
     1921 tctcaacatc aagtcgctcg ccattcgcct gcacggcgac ggcccgaagc gggtgagcct
     1981 caaggccacc ggcccgggcg aggtgcgcgc cggccagatc gagaccggcc acgacatcga
     2041 gatcatgaac aaggatctgg tgatctgcac gctcgaccag agcgcgaaga tca
//
LOCUS       DASUJG010000220         2335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1655955, whole genome shotgun sequence.
ACCESSION   DASUJG010000220 DASUJG010000000
VERSION     DASUJG010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655955"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..142
                     /locus_tag="VFX38_04675"
     tRNA            67..142
                     /locus_tag="VFX38_04675"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:100..102,aa:Ala,seq:ggc)
     gene            193..268
                     /locus_tag="VFX38_04680"
     tRNA            193..268
                     /locus_tag="VFX38_04680"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:227..229,aa:Glu,seq:ttc)
     gene            complement(479..>2335)
                     /locus_tag="VFX38_04685"
     CDS             complement(479..>2335)
                     /locus_tag="VFX38_04685"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5314191.1"
                     /translation="FGIAWDSRPRSAGGQRWFALYPGTGAAPSGALNWTAPDQTWNYQ
                     CAACHSTDLHKNYDLATHSYATTYSEVSVGCEACHGPGSAHVAWAKDPHHPPQLAATE
                     GLTIALDERQGVTWKTDLATGLLKRSRPRTSDREIETCAECHARATQIHRDYVHGQPL
                     GDDYRVALLDDDLYFPDGQIKGEVYEYGSFLQSRMFHAGVSCSDCHNPHTATLRAQGN
                     RLCTGCHLADKYDSPRHYHHPVGSTGAQCVACHMPTRTYMRIDRRRDHSIRVPRPDFS
                     VAIGTPNACNECHVHRSAQWAAEHITKWFGPRRIGFQRFAAALHAGEIGAPGSYRQLA
                     NLVASSAQPAIARATAITLLAAYGEPQTATLLQRESHDPSALVRRATAEALANANRAE
                     LTPFLRLLGDPVRSVRIAAADALAEIDRKALSAPTIDAFNKASAEYLAAQQLDADRPE
                     AHLDLALYFSRQNRLDRAKSELATALALDPGFAPAAVNLADLYRSLGQDDRAGQVLQA
                     SLKRAPDNPALLFSLGLLDIRKGAKSPALEFLQEASRNAPDNARYAYVYALALEDAGN
                     TETAIEVLEQSLQTNPYDRESLSALASFYTRIGNPAAAAKYQRVLARLTADP"
BASE COUNT          392 a          687 c          807 g          449 t
ORIGIN      
        1 tcaggcgtca ggtaatcaat tatcggaaag tgcgctatcc tgatcactga tacctgatac
       61 ctgaacgggg ccatagctca gctgggagag cgcctgcatg gcatgcagga ggtcggcggt
      121 tcgatcccgc ctggctccac catttcgcga ggacatcctg gtaacgggat gatttcttgt
      181 actttttcta atgtccccgt cgtctagagg cccaggacat cgccctttca cggcggcaac
      241 acgggttcga ctcccgtcgg ggacgccatt ttccgcttgc taatccggcc cggcctgtgt
      301 gggctttcgg atccgaatgc gccacggtgc gctggcaggg tgttgaaaaa caccctgcta
      361 aggcgggacc ccgcccgcga atcaagcgcg caggcgcttg attcgtcgtg agcgaatccg
      421 gacatctgcc ggattcgcga cttgaaaaag ggcaggacag ccctttttca acaaacagct
      481 acgggtcggc cgtcaatctc gcgagaaccc gttgatattt tgcggccgcc gcaggattgc
      541 cgatgcgcgt gtagaagctc gcgagcgcgg agagcgactc gcgatcgtac gggttggttt
      601 ggagcgattg ctcgagaacc tcgatcgcgg tctccgtatt gccggcatcc tcgagcgcga
      661 gcgcgtacac gtaggcgtag cgggcgttgt ccggcgcgtt ccgcgaggct tcttgcagga
      721 attcgagcgc cgggctcttc gcgcctttgc gaatatcgag cagtccgagc gaaaagagca
      781 acgccggatt gtccggggcg cgcttcagag acgcctgcag aacttgcccg gcgcgatcgt
      841 cctgtccgag cgagcgatac agatcggcga gattgaccgc ggctggtgcg aagcccggat
      901 caagagcaag cgccgttgcg agttccgatt tcgcacggtc gaggcggttc tgtcgggaga
      961 aatagagcgc gagatcgaga tgcgcctccg gcctgtcggc gtcgagttgc tgcgccgcga
     1021 gatactcggc ggacgctttg ttaaatgcgt cgatcgtcgg tgctgagaga gccttgcgat
     1081 cgatttctgc gagcgcgtct gccgcggcga ttcgaaccga acggaccgga tcgccaagca
     1141 gccgaaggaa tggcgtgagt tctgcccgat tcgcgtttgc gagggcctcg gcggttgcgc
     1201 gccgcaccag cgcggaggga tcgtgcgatt ctctttgcag gagcgtcgcg gtttggggtt
     1261 cgccgtaggc ggcaagaagc gtgatcgcgg tggcccgcgc gatcgccggc tgcgcggaac
     1321 tcgcgacgag gttcgcgagc tgccggtacg aaccgggcgc gccgatttcg ccggcgtgca
     1381 gcgccgcggc gaatcgctga aagccgatgc gacgaggtcc gaaccacttc gtgatgtgct
     1441 ccgcggccca ctgcgcgctc cgatggacat gacattcgtt gcaggcgttc ggagtgccga
     1501 tcgccaccga gaaatcgggg cggggaacgc ggatgctgtg atcgcgccgc cggtctatgc
     1561 gcatgtaggt tcgggtcggc atgtggcagg cgacgcactg cgcgcccgtc gatccgacgg
     1621 gatgatggta gtggcgcggg ctgtcgtact tgtccgcaag atggcaaccg gtgcagagcc
     1681 ggttgccctg cgcgcgcagc gtcgcggtgt ggggattgtg gcagtcgctg cagctcacgc
     1741 cggcatgaaa catgcggctc tggaggaacg agccgtattc gtacacctcg cccttgatct
     1801 ggccgtccgg gaaatagaga tcgtcgtcga gcagcgcgac gcggtagtcg tcgccgagcg
     1861 gctggccgtg gacgtaatcc cggtgaatct gcgtcgcccg cgcatgacat tccgcacagg
     1921 tttcgatttc gcggtcgctg gtccgcggcc ggcttcgctt cagcagaccg gtggcaagat
     1981 cggtcttcca cgtcacgccc tgtcgttcgt cgagggcgat ggtcagacct tccgtcgcgg
     2041 cgagttgggg aggatgatgc gggtccttgg cccaggcgac gtgggccgag cccggaccgt
     2101 gacaggcttc gcagccgacg ctcacttccg aatacgtggt cgcgtagctg tgtgtcgcga
     2161 gatcgtaatt cttgtgcaaa tcggtggaat ggcaggcggc gcactgatag ttccaggtct
     2221 gatccggcgc cgtccagttc aaggcgccgg agggagcggc gccggttccc ggatacagcg
     2281 cgaaccagcg ttgcccgccg gcgcttcgcg ggcggctgtc ccaggcgatg ccgaa
//
LOCUS       DASUJG010000221         6981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1044626, whole genome shotgun sequence.
ACCESSION   DASUJG010000221 DASUJG010000000
VERSION     DASUJG010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6981
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044626"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1354)
                     /locus_tag="VFX38_04690"
     CDS             complement(<1..1354)
                     /locus_tag="VFX38_04690"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX5314192.1"
                     /translation="MRLISVLAALLLGALPLASANAATAAAPASAAIAASAGGQDLFN
                     LNLPKLGLAGGGAFPLWKAEAIGRQIYYAIQQEDGVLNDPLVADYVDYLGHQLSSVAN
                     APDEPFHYFVVLDPQINAFALPGAFIGVNSGLFEATRTEDELAGVLAHETAHVVQRHI
                     ARQVADAHYNNLINLAILLGAIAVAAANPDLSMAAISGAQGGIAQRQINYTRADELEA
                     DRVGISILARAHFNPQGMIGFFQYMQRQYAMQGFQIPEFLSTHPLDITRVSEAQQRAR
                     DLHVKPRAENPNFALMKARVRVLTSDDPVATLAYYRVQSQSASNPWYREAAKYAMVLC
                     MSQLNQGKEAVALAKPLAAAHPDNIALQLALAQAMIAAGDTKAGLAALAQDNRLYGPS
                     MAVAMTYAQALYDAGDAREAVGVLQPLAQLDGTDPIYDPDFYQLLGSAANKIGNHSLA
                     Y"
     gene            complement(1452..2189)
                     /locus_tag="VFX38_04695"
     CDS             complement(1452..2189)
                     /locus_tag="VFX38_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HEX5314193.1"
                     /translation="MSESFAPARLWCRERLADPAEDLGVALPFAPRHSRAALTALGAM
                     YVEFETIATRFRELQVARSKLGWWRDELARLDAGRPEHPATRLLAASGVAIPLVAIGD
                     LLGGFELILLGGPVNDLATATLRSELGFARFCFLIAQLEAHDSAPPELLARLGLALGL
                     TRILRSPALDASARNTIARAARDSLAGCLGGLESLPQPLPPLAALARGDLERDPLAQL
                     GRKARSARVLCAWRAARGGLPRSLRSA"
     gene            complement(2186..2863)
                     /gene="gph"
                     /locus_tag="VFX38_04700"
     CDS             complement(2186..2863)
                     /gene="gph"
                     /locus_tag="VFX38_04700"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEX5314194.1"
                     /translation="MNPRLVLFDLDGTLADTAADITRATNRVLAEVERPPLPLATVRA
                     LVSKGARAVLRAGFDVPPDPAALDALAQRLFEVYCEAPVVDTRPFPGLRGVIADLGEA
                     GLMWGVVTNKPIRLADPIVAALALQPAPRLVIGGDSLARRKPDPLPLLAACERAGVAI
                     AESIYVGDAEIDVQTARAAGMPVVIAGFGYALPHDANWDPAPDFYARSVDELASILLA
                     PVSSTGS"
     gene            complement(2860..3567)
                     /gene="ubiG"
                     /locus_tag="VFX38_04705"
     CDS             complement(2860..3567)
                     /gene="ubiG"
                     /locus_tag="VFX38_04705"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEX5314195.1"
                     /translation="MTERNADNAELAHFGALASRWWDPLGEFRTLHHINPARLEFVRR
                     HAPLAGTAAVDVGCGGGILSEALAAEGAHVTGIDLAAPLLEVARLHAIESGCTIEYVE
                     TSAERLAQERPAAFALATSMELLEHVPDPVSVVAACAALLAPGGIAVFSTLNRTFKAW
                     ALAIVGAEYLTNLVPRGTHAYERFIRPSELDAWARAAGLELLEITGLEYEPWRRSARL
                     GGDAGVNYLAAYRRNAQ"
     gene            complement(3560..4879)
                     /locus_tag="VFX38_04710"
     CDS             complement(3560..4879)
                     /locus_tag="VFX38_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009147413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HEX5314196.1"
                     /translation="MQAELLIRARWVAPVDPAQLLDDHAVAVSAGRIEAILSAAEAER
                     SIEAHETIRLDDHLLIPGLVNAHAHSAMTLLRGYADDLPLDAWLKERIWPAEARFADE
                     TFVRDGALAAAAEMLAGGTTTFADMYFFPEATIESALALGMRVVSGIVVIGFATRYAG
                     EPGAYLERGLALRDACRANPLVSFMLAPHAPWSVNEETLRRIATIANETEMPIQTHLH
                     ETAEEIATSLAAQGRRPLARLAELGLTGPALAAVHMTQVNDEDLEGLSRDGVHVIHCP
                     VSNLKLASGFCPVTRLLDAGVNVALGTDGAASSGDLDLILEMRTAALLAKGVAGDASA
                     LPAPLALHAATLGGARALGIDDRVGSLVPGKEADMTAIDFGAARLQPVYDPLSQLVYA
                     ASARDVSDVWVAGARRVENGVLQDLDESRLRSRLQQWGRRIAGAKDD"
     gene            5050..>6981
                     /gene="gyrA"
                     /locus_tag="VFX38_04715"
     CDS             5050..>6981
                     /gene="gyrA"
                     /locus_tag="VFX38_04715"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX5314197.1"
                     /translation="MVDFAKELSNITLEEEMQRSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMKQLGNDYNKPYKKSARVVGDVIGKYHPHGDTAVYDTIVRMAQSFSMRNVL
                     VDGQGNFGSVDGDAPAAMRYTEVRMAKLAHEILADLDKETVDFQPNYDGSETEPTVMP
                     TRVPNLLVNGSAGIAVGMATNIPPHNLAETVNAAIALLDDPEIGLEGLMEHLPGPDFP
                     TAGILSGRRGIVEAYRSGRGRIYLRARTQYEERAHGGHPAIIVTELPYQVNKARLIEK
                     IADLVRDKRLEGIAELRDESDKDGMRVVIELRRDALPEVLLNNLYLHTQMQVVYGINM
                     VSLLDGQPKLLGLKEMLEAFLRHRREVVTRRTVFELGQARKRAHVLEGLAVALANVDA
                     VIALVKAAKTPPEAKEALLARDWEPGAVVGLLENVDADLTRPADAPPECGLHAGRYRL
                     SPTQAQAILDMRLQRLTGLEQDKIISEYHEIIATIRALREILADPGRLRGVIREELRA
                     LATEFGDPRRTEISALHTEFEDEDLIADEEVVVTLSHAGYVKYQMPDVYQAQHRGGRG
                     RRAASVKEEDFVEQLFIARTHDTLLCFSSAGKLYWLKVYKLPQAGRGSRGRPIVNLLP
                     LEEGERITAILSVREYDSGH"
BASE COUNT         1212 a         2209 c         2453 g         1107 t
ORIGIN      
        1 ggtaggcgag gctgtggttg ccgatcttgt tggcggccga accgagcagt tgatagaagt
       61 ccggatcgta aatcggatcg gtgccgtcga gttgcgcgag cggttgcagc acgcccaccg
      121 cttctcgcgc gtcccccgca tcgtacagcg cctgggcgta ggtcatggcg acggccatgc
      181 tgggaccgta cagccggttg tcctgggcga gcgccgcgag ccccgccttg gtgtcgccgg
      241 cggcgatcat cgcctgggcg agcgcgagct gcagcgcaat attgtcggga tgagccgccg
      301 cgagcggctt tgcgagcgcc accgcctctt tgccctgatt caactgcgac atgcacagca
      361 ccatggcgta cttcgcggcc tcgcggtacc agggattgct cgcggattgg gactggaccc
      421 ggtagtaggc gagagtcgcc acgggatcgt cgcttgtgag cacccgcacg cgggccttca
      481 tgagcgcgaa gttgggattt tcggcgcgcg gcttcacatg aagatcgcgc gcccgctgct
      541 gcgcttcgga gacgcgggtg atgtcgagcg gatgcgtgct cagaaactcg ggaatctgaa
      601 agccctgcat ggcatattgc cgctgcatgt actggaagaa gccgatcatt ccctgcggat
      661 tgaaatgcgc gcgggcgaga atcgagatgc cgacgcgatc ggcttcgagt tcgtccgccc
      721 gcgtgtagtt gatctggcgt tgcgcgatcc cgccctgggc gccggagatc gccgccatcg
      781 acagatcggg attggcggcg gcgacggcga tggcgccgag gagaatcgcg aggttgatga
      841 ggttgttgta atgcgcgtcc gcgacctggc gggcgatatg ccgctggacc acgtgcgcgg
      901 tttcatgcgc gagcacgccg gcgagttcgt cctcggttcg cgtcgcctcg aagagcccgg
      961 agttgactcc gatgaaggcg ccgggaagtg cgaaggcgtt gatctgggga tcgagcacga
     1021 cgaaataatg gaagggttcg tcgggagcgt tggcgaccga ggaaagctga tggccgaggt
     1081 aatccacgta gtcggcgacg agcggatcgt tgaggacgcc gtcttcctgc tggatcgcgt
     1141 agtaaatctg ccggccgata gcttcggcct tccacagcgg gaaggcgccg ccgccggcga
     1201 gtccgagctt gggaaggttg aggttgaaga ggtcctgacc gccggcgctg gccgcgatcg
     1261 cggccgaggc gggagcggcc gcggtcgccg cattcgcgct tgcgagcgga agcgctccga
     1321 ggagcagggc ggcgaggacg gagatcaggc gcatggggaa actgtacaag ccacgggcgc
     1381 ttggaccggc tgccgcggca ttttacgccc gccgcggcga aatgcccgca cagcccgctc
     1441 ggccgccgga ttcaggcgct tcgcaggctt cgcggcaagc cgccgcgcgc cgcgcgccag
     1501 gcgcaaagaa cgcgggcgct gcgagccttc cgtccgagct gtgcgagcgg atcgcgctcg
     1561 agatcgccgc gggcaagggc cgcgaggggc gggagcggtt gcggcaggga ttcgagcccc
     1621 ccgagacatc ccgcgagcga atcccgggcg gcgcgagcga tggtgttccg cgccgacgcg
     1681 tcgagcgcag gcgagcgcag gatccgggtg aggccgagcg caagcccgag gcgggcgagc
     1741 aattcgggag gcgccgaatc gtgcgcttcc aactgcgcga tgagaaagca gaagcgtgcg
     1801 aagccgagtt cgcttcgcaa ggtcgcggtc gcgaggtcgt tcaccggccc gccgaggaga
     1861 atcaattcga agccgccgag aagatcgccg atggcgacga gcggaatcgc gacgccgctc
     1921 gcggcgagaa gccgtgttgc cggatgctcc gggcgcccgg catcgagacg cgcgagttcg
     1981 tcgcgccacc agccgagttt gctgcgcgcg acctggagtt cgcgaaagcg ggtcgcgatc
     2041 gtttcgaact cgacgtacat cgcgccgagc gccgtgagtg cggcgcgcga gtggcgcggc
     2101 gcaaacggca gggcgacgcc caagtcttcg gccggatcgg cgagccgttc gcgacaccag
     2161 aggcgggccg gcgcgaaact ctcgctcacg atccggtgga ggagaccggt gccaggagga
     2221 tgctagcgag ttcgtcgacg ctccgcgcgt aaaaatccgg cgccggatcc caattcgcgt
     2281 cgtgagggag cgcatacccg aagcccgcga tgacgacggg catgccggcc gcgcgcgcgg
     2341 tttgcacgtc gatttcggcg tcgccgacgt agatgctctc ggcaatcgca acgcccgcac
     2401 gttcgcaggc cgcaagaagc ggcagcggat cgggcttgcg ccgcgcgagg ctgtccccgc
     2461 cgatgacgag ccgcggtgcc ggttgcagcg cgagcgcggc gacgatcgga tcggcgagcc
     2521 ggatcggctt gttcgtcacc acgccccaca tgagacccgc ttcgccgaga tcggcgatca
     2581 cgccgcgaag gcccgggaac gggcgcgtgt cgacgaccgg cgcttcgcag tacacctcga
     2641 acagtcgctg cgcgagagcg tccagcgccg caggatcggg cgggacgtcg aagcccgcgc
     2701 gcagcacggc gcgtgcgcct ttggacacga gtgcacgcac cgtcgcgagc ggcaggggcg
     2761 gacgctcgac ttccgcgagc acgcgattgg tggcgcgcgt gatgtcggcc gcggtatcgg
     2821 caagtgtgcc gtcgagatcg aagagaacga ggcgcggatt cattgggcgt tgcggcgata
     2881 ggcggcgaga tagttgacgc cggcgtcgcc gccgaggcgc gccgatcgcc tccagggttc
     2941 gtattccagt ccggtgattt cgagcagttc gagtcccgcc gcgcgtgccc aagcgtcgag
     3001 ttccgagggg cggatgaagc gttcgtaggc gtgggtgccg cggggcacga gattggtcag
     3061 atattcggcg ccgacgatcg cgagcgccca ggccttgaac gtgcggttga gcgtcgaaaa
     3121 aaccgcgatg ccgccgggcg cgagaagtgc ggcgcaggcg gcgacgacgg agaccggatc
     3181 ggggacgtgt tcgaggagtt ccatcgaggt cgcgagcgcg aacgccgccg gccgttcctg
     3241 ggcgaggcgc tcagccgacg tttcgacgta ttcgatggtg caccccgatt cgatcgcgtg
     3301 caggcgcgcg acttcgagca gcggcgccgc gagatcgatg ccggtgacgt gcgcgccctc
     3361 cgcggccagc gcttcgctca ggattccgcc gccgcagccg acgtccaccg ccgccgtgcc
     3421 ggcgagcggc gcatggcggc gcacgaattc gagccgcgcg ggattgatgt gatggagcgt
     3481 gcggaattcg cccagcgggt cccaccagcg cgaggcgagc gcaccgaaat gcgcgagttc
     3541 ggcgttgtcg gcgttgcgct cagtcatcct ttgctcccgc gatccggcgt ccccattgtt
     3601 gcagacgcga gcgcagtcgg gattcgtcga ggtcttggag aaccccgttt tcgacgcggc
     3661 gcgcgccggc gacccagacg tcgctcacgt cgcgagcgct tgccgcatag acgagctgcg
     3721 agagcggatc gtaaaccggt tgcagccgcg cggcgccgaa atcgatcgcg gtcatgtcgg
     3781 cctccttgcc ggggacaagg ctccccacgc gatcatcgat cccgagcgca cgcgcgccgc
     3841 ctagtgtcgc cgcatgaagc gcgagcggcg ccggcagcgc gctggcgtcg ccggccacgc
     3901 ccttcgcgag gagcgccgca gtgcgcattt cgagaatgag atcgagatcg ccgctgctcg
     3961 ccgcgccgtc ggtgccgagc gcgacgttca cgccggcgtc gagaagacgc gtgaccggac
     4021 agaatccgct cgcgagtttg agattcgaca ccgggcagtg aatgacgtga accccgtcgc
     4081 gggaaagccc ttcgagatct tcgtcgttca cctgtgtcat gtggaccgcc gcgagcgcgg
     4141 gcccggtgag gccgagttcc gcgagacggg cgagcgggcg cctgccctgc gcggcgagac
     4201 tcgttgcgat ctcctccgcc gtttcgtgca aatgcgtctg gatcggcatt tccgtttcgt
     4261 tcgcgatcgt cgcgatccgc cggagcgtct cctcgttcac gctccaggga gcgtgcggag
     4321 cgagcatgaa cgacaccagc ggatttgccc ggcaggcatc gcgcagcgcg aggccgcgtt
     4381 cgagatacgc gcccggctct cccgcgtagc gcgtggcaaa cccgatcacc acgattcccg
     4441 agacgacgcg catgccgagc gcaagcgccg attcgatcgt cgcctcgggg aagaaataca
     4501 tgtcggcgaa ggtcgtggtt ccgcccgcga gcatttctgc cgcggccgcg agcgcgccgt
     4561 cgcgcacgaa tgtctcgtcg gcgaagcgcg cctcggccgg ccagatccgc tccttgagcc
     4621 aggcatcgag cggaagatcg tcggcatagc cacgcaggag cgtcatcgcg gaatgcgcgt
     4681 gggcattgac gaggccggga atgagaaggt gatcgtcgag acggatcgtc tcatgcgcct
     4741 cgatgctgcg ttcggcctcg gctgcggaaa gaatcgcctc gattcgcccc gcactcaccg
     4801 cgaccgcgtg gtcgtcgagc agttgcgccg gatcgaccgg ggcgacccag cgtgcgcgaa
     4861 tcaacagttc ggcctgcacc ggctttactc cggcgatcgg cgcagacagg gcagagatcg
     4921 ggcggcggcg ctattcatcg gctgcaagta taattcccgg cctttgcggc agcgatttgc
     4981 acggcgccgc gggccgccgt cggatcgccc gtcatggtaa aataaacaag ataactcaag
     5041 gacttgaata tggtcgattt cgccaaggaa ctctccaaca tcaccttgga agaggagatg
     5101 cagcgctcct acctcgatta tgcgatgagc gtgatcgtgg gccgtgcgct gcccgacgtg
     5161 cgcgacggcc tgaaacccgt gcatcggcgc gtgttgtacg cgatgaaaca gctcggaaac
     5221 gattataaca agccgtacaa gaaatccgcc cgcgtggtcg gcgacgtcat cggcaaatat
     5281 catccgcacg gcgacaccgc cgtgtacgac accatcgtgc gcatggcgca atccttttcg
     5341 atgcgcaacg tcctcgtgga cgggcaggga aacttcggtt ccgtggacgg cgatgcaccg
     5401 gccgccatgc gctacaccga ggtgcgcatg gcgaagctcg cgcacgaaat tcttgcggat
     5461 ctcgacaagg aaacggtcga ttttcaaccg aactacgacg gatcggaaac cgagccgacg
     5521 gtgatgccga cgcgggttcc gaatcttctc gtcaacggct cggcgggcat cgccgtcggc
     5581 atggcgacca atatcccgcc gcacaatctg gcggagacgg taaacgccgc gatcgcgctg
     5641 ctcgacgatc ccgagatcgg gctcgaggga ctcatggagc atctgccggg gccggatttt
     5701 ccgacggcgg gcattctctc cggacgacgc ggcatcgttg aggcgtatcg cagcggccgc
     5761 gggcgaattt atctgcgtgc ccgcacgcaa tatgaagagc gcgcgcacgg cgggcacccc
     5821 gcgatcatcg tcaccgaact tccctatcag gtgaacaagg cgcggctgat cgagaagatc
     5881 gcggacctcg tgcgcgacaa gcgcctcgag ggcatcgcgg agctgcgcga cgaatccgac
     5941 aaggacggca tgcgcgtggt gatcgaactg cgccgcgatg cgctccccga ggtgcttctg
     6001 aacaatctct atctgcacac gcagatgcag gtcgtctacg gcatcaacat ggtctcgctg
     6061 ctggacggcc agccgaaact tctcggcctg aaggagatgc tcgaggcgtt tctccgtcat
     6121 cgccgcgagg tcgtcacccg gcgcacggtg ttcgaactcg gccaggcgcg caaacgagcc
     6181 catgttctcg aagggctcgc cgtcgcactt gccaacgtgg acgccgtgat cgcgctcgtg
     6241 aaggcggcaa agactccgcc ggaggcgaaa gaggcgctcc tcgcgcgcga ctgggaaccg
     6301 ggagcggtcg tggggcttct ggagaacgtg gacgccgatc tcacgcggcc ggcggatgcg
     6361 ccccccgagt gcggcctgca cgcgggacgc tatcggcttt cgcccacgca ggcgcaggcg
     6421 atcctcgaca tgcgcctgca gcgcctgacc ggcctcgagc aggacaagat catctcggaa
     6481 tatcacgaaa tcatcgcgac catccgggcg ttgcgggaaa ttctcgccga tcccggacgt
     6541 ctgcgcggcg tcattcgcga ggaactgcgg gcgctcgcga ccgaattcgg cgatccccgg
     6601 cgcaccgaaa tctcggcgct tcataccgag ttcgaagacg aggatctcat cgccgacgag
     6661 gaggtcgtgg tcacgctttc gcacgcgggg tacgtcaagt accagatgcc ggacgtctat
     6721 caggcgcagc atcgaggcgg gcgcggccgg cgtgccgcgt cggtcaagga agaggacttc
     6781 gtcgagcagc ttttcatcgc ccgcacgcac gacaccctgc tttgcttttc cagcgcaggc
     6841 aagctctact ggctcaaggt gtacaagctg ccgcaggccg gacggggctc gcggggacgg
     6901 ccgatcgtga atctcctgcc gctggaagag ggcgagcgga tcacggcgat tctttccgtt
     6961 cgcgaatacg attccggaca c
//
LOCUS       DASUJG010000222         5567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_65781, whole genome shotgun sequence.
ACCESSION   DASUJG010000222 DASUJG010000000
VERSION     DASUJG010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5567
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65781"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /gene="ccmI"
                     /locus_tag="VFX38_04720"
     CDS             complement(<1..557)
                     /gene="ccmI"
                     /locus_tag="VFX38_04720"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HEX5314198.1"
                     /translation="MSFLIAAGLLLLAVLLLLGWPLLRRVRRSAPDDVHGRLVAELDD
                     DVAAGVLPAEDRALAARDLEPVAKEELHSPRRSGRWAWLALAAVPLVAVVLYWQLGNW
                     RAGIEGNRAAVLHEADQMLAELRVHLKSDPRDAAGWITLGRAESAMGNYPSAASAYGR
                     AVQVDGSRDPELLAAWGEAQVLADPA"
     gene            complement(554..1078)
                     /locus_tag="VFX38_04725"
     CDS             complement(554..1078)
                     /locus_tag="VFX38_04725"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEX5314199.1"
                     /translation="MTIPKLGVVGWRLGKAGILSPLAVFGKRGVGGGILATIVMLLLS
                     AAPAFAAPAPSGPDVLPPKLELRYQKLTSELRCPVCQNETIAVSDSVVSADLRRVVRE
                     KLRAGASDREIRDYMIARYGLFAVYDPPVAAGTWALWFGPLALLLLAALIAAIAIRRR
                     RRILAGRERAEFGP"
     gene            complement(1075..1611)
                     /locus_tag="VFX38_04730"
     CDS             complement(1075..1611)
                     /locus_tag="VFX38_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HEX5314200.1"
                     /translation="MRRWLWALLPLAVAGGLIAFLALGFGHDPRYIPSPLIDKPAPKF
                     ALQTLADPARSLSSADLHGQVALVNVFASWCVACTDESASLAWLAGQGVRIYGLDYSD
                     TRPAAKAWLARWGNPYRAVAFDPRGTAALDWGIYGVPETFALDRNGRIRHKFTGVITL
                     EAAKSEVLPLLRKLESGS"
     gene            complement(1611..3572)
                     /locus_tag="VFX38_04735"
     CDS             complement(1611..3572)
                     /locus_tag="VFX38_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEX5314201.1"
                     /translation="MVPELGQFALMLALALAVAQAVSGFAGASRRSPRLLALVRPAAC
                     GQFLAVATAFGCLTWAMVHTDLTVEYVAENSNALVPLLYKFTGVWGAHAGSLLLWTFI
                     LSVWGVAVAAFSRRLPAVFAGRVLAVMGVIGIGFLAFTIWLSNPFARALPPPASGADL
                     NPILQSPLLAIHPPMLYMGYVGFSVAFAFAIAALIGGRMDTAWARWTRPWTLVAWLFL
                     TAGITIGSFWSYLELGWGGWWFWDPVENASFMPWLAGTALIHSLVVTEKRGAFKGWTA
                     LLAIFVFSLSLLGTFLVRSGVLVSVHAFAVDPRRGIYVLALLGIALFGGLALYAWRAP
                     RFVGGGGFAFLSRETLILANNVILVVACAAVLLGTLYPLFFEVVGAGRISVGPPYFDK
                     VFLPLMVPFFLLIGIAPSIRWRRGDRGELWRRLRWPLALAIALAIAVAAATWQGASAL
                     TIAGVALGLWVIVSAWAEVLRRMRQAPAGRRLHLSRGAWGMTLAHFGAGILVLGVTVS
                     TTMSSYLDTSLRPGQSATVGGYRFDFVRDGSVAGPNYQGEQGTFRVSRNGEEVAMLRP
                     ESRVYKGGTPTSESAISWKPTHDIYIAMGQPIGDDAWSVRLQVRPFVRFIWFGGWLIA
                     FGGLLALSDPRYRRREELRLPQGVGAETS"
     gene            complement(3562..4032)
                     /gene="ccmE"
                     /locus_tag="VFX38_04740"
     CDS             complement(3562..4032)
                     /gene="ccmE"
                     /locus_tag="VFX38_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017683763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEX5314202.1"
                     /translation="MTPRRQRLLVVVGILAGTAIGVGVALKAFSGNLLYYYTPTQTLE
                     KNLGPKRVFRLGGLVEKGSVKRQPGSLTVRFVVTDNRRSIPVIFTGVLPDLFRPGQGV
                     IANGHMGPGGVFVANDVLAKHDSKYMPLKLYNKENGTVYRKPGSMDAGAGGRGS"
     gene            complement(4029..4193)
                     /gene="ccmD"
                     /locus_tag="VFX38_04745"
     CDS             complement(4029..4193)
                     /gene="ccmD"
                     /locus_tag="VFX38_04745"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HEX5314203.1"
                     /translation="MRSLHEFLTMGGYALYVWPAYAAAFVVMAGSAWLALRGLRRARE
                     RAIRHLEDEA"
     gene            complement(4201..4932)
                     /locus_tag="VFX38_04750"
     CDS             complement(4201..4932)
                     /locus_tag="VFX38_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HEX5314204.1"
                     /translation="MFVWFHKLGSPPVLFRLAARTAPWFQWIGLALIVAGLIGGLVLA
                     PPDYQQGDGFRIIYIHVPSAWLSMLLFAIMALASIAGLVWRLRMAHAITAAAAPIGAA
                     FTFLALLTGSLWGKPMWGTYWVWDARLTSELILFFIYLGYIALANAFSDQRTGDRAGA
                     ILAIVGAVDVPIIHYSVQWWTTLHQGASLSLSGSAIAPSMLWPLLVMIVGFTCYSAWL
                     ITLRLRSEILRRNRRSRGMAQRMLR"
     gene            complement(4981..>5567)
                     /gene="ccmB"
                     /locus_tag="VFX38_04755"
     CDS             complement(4981..>5567)
                     /gene="ccmB"
                     /locus_tag="VFX38_04755"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX5314205.1"
                     /translation="FLVTSMLFPLGLSAAPDLLRPLAAGVIWVIAFLAAMLSLQGFFR
                     VDYEDGTLEQLLLTPRPLSLVILAKASGHWILTGLPIVCGAALVAVMLYVPGRGIAAL
                     VAGLLLGTPVLTLLGTLVAALTVSLRQAAVLAPVLILPLAVPVLIFGAHSATLAMAGL
                     PLGQAFAALAALLILALSLLPPAAAAALRISHES"
BASE COUNT         1120 a         1886 c         1826 g          735 t
ORIGIN      
        1 gcgggatcgg cgagcacctg cgcctcgccc caggccgcga gaagttccgg atcgcggctg
       61 ccgtcgactt ggaccgcacg accataggca ctcgccgccg acggataatt gcccatcgca
      121 ctttccgcgc gacccagggt gatccagccc gccgcgtccc tcggatcgct tttgaggtgg
      181 acgcgcagct cggcgagcat ctgatccgcc tcgtgcagaa cggcggcgcg gttgccttcg
      241 attcccgcgc gccaatttcc cagctgccag tagagaacga cggctacgag cggaaccgcc
      301 gcgagcgcga gccacgccca gcgcccggaa cgacgcggcg aatgcaactc ctcctttgcc
      361 accggttcga gatcgcgggc ggcgagggcg cgatcctcgg cgggcagcac tcccgccgcg
      421 acgtcgtcgt cgagttcggc gacgagtcgt ccgtgcacgt catccggcgc ggaacgccgc
      481 acgcggcgca ggagcggcca gccgagcagc agcagcaccg cgagcagcag aagccccgcc
      541 gcgatcagga aactcatggc ccgaactccg cgcgctcacg accggcgaga atccgtcgcc
      601 ggcggcggat cgcgatcgct gcgatgaggg cggcgagcag gagcagcgcg agcgggccga
      661 accagagcgc ccaggttccc gccgcgaccg gcggatcgta caccgcgaag agtccgtagc
      721 gcgcgatcat gtaatcgcgg atttcgcgat cgctagcgcc cgcgcgcaac ttctcgcgca
      781 cgacgcgacg cagatcggcg gaaaccacgg aatcggaaac cgcgatggtt tcgttctggc
      841 agaccgggca gcgcaattcg ctcgtgagtt tctggtagcg cagttcgagt ttcggcggca
      901 gaacgtcggg gccgctcggc gccggtgctg cgaatgcggg cgccgctgac aacagcagca
      961 tgacgatcgt tgcaagaatc ccccctccaa ctccccgctt cccgaagacg gcgagagggg
     1021 agagaattcc cgctttcccg agcctccagc ccacgactcc cagctttggg attgtcacga
     1081 tcccgactcc aacttgcgca gcagcggcaa cacttcgctc tttgcggcct cgagcgtgat
     1141 cacgccggtg aacttgtgtc ggatgcgccc attgcgatcc agcgcgaagg tttccggcac
     1201 gccgtaaatt ccccagtcga gtgccgcggt gccgcgcgga tcgaaggcga cggcgcgata
     1261 gggattgccc cagcgcgcaa gccaggcctt cgccgccggg cgcgtgtccg aatagtcgag
     1321 tccgtagatg cgcacgccct gccccgcaag ccaggcgagc gatgcgcttt cgtcggtgca
     1381 ggcgacgcac cagctcgcga acacgttcac gagcgccacc tgcccgtgca gatcggcgct
     1441 gctcaagctg cgcgcgggat cggcgagcgt ctgcagcgcg aacttgggcg ccggcttgtc
     1501 gatgagcggc gagggaatgt agcgcggatc gtggccgaag ccgagcgcga gaaaagcgat
     1561 gaggccgccg gccaccgcga gcggcaaaag agcccacagc cagcggcgca tcaggaagtc
     1621 tccgcaccga ctccttgcgg aagtcgcagc tcctcgcggc ggcggtaacg cggatcgctg
     1681 agcgcaagga gtcctccgaa cgcgatcagc cagcctccga accagatgaa gcgcacgaag
     1741 gggcgcacct gcagacgcac actccaagca tcgtcgccga tcggctggcc catcgcgatg
     1801 tagatgtcat gcgtgggctt ccaggaaatc gccgattcgc tggtcggcgt tccgcccttg
     1861 tacacgcgcg attccgggcg cagcatcgcg acttcttcgc cattgcggct cactcgaaac
     1921 gttccttgct cgccctggta attgggcccc gcgaccgagc cgtcacgtac gaaatcgaag
     1981 cggtagccgc cgaccgtggc gctttgtccg ggccgcagac tggtgtcgag ataactcgac
     2041 atcgtcgtcg agaccgtcac cccgagcacg aggatgccgg cgccgaaatg cgcgagcgtc
     2101 attccccagg cgccgcgcga gagatgcagg cgtcgccccg ccggagcctg gcgcatgcgc
     2161 cgcagcactt ccgcccaggc actcacgatc acccagagcc cgagcgcgac tccggcgatc
     2221 gtcagcgccg atgctccctg ccaggtcgcg gccgcgacag caatcgcgag cgcgatggcg
     2281 agcgcgagcg gccagcgcag ccgccgccac agttcgcccc ggtcgccgcg acgccagcga
     2341 atgctcggcg caatgccgat cagcagaaag aacggcacca tcagcggcag gaagaccttg
     2401 tcgaaatatg gcggaccgac cgaaatcctt ccggctccca cgacctcgaa gaagagcgga
     2461 tagagcgtgc cgagcaggac cgccgcgcag gcgacgacga gaatcacgtt gttcgcgagg
     2521 atcagggttt cgcgcgagag aaacgcgaag ccgccgccgc ccacgaaacg cggcgcgcgc
     2581 caggcataga gcgcgagccc gccgaagagt gcgatgccga ggagcgcgag tacgtagatg
     2641 ccgcggcgcg gatcgaccgc gaaggcgtgc accgacacga gcacgcccga gcgcacgaga
     2701 aacgttccga gcagcgacag cgaaaacacg aagatcgcga ggagcgccgt ccagcccttg
     2761 aaggcgccgc gcttctcggt caccacgagc gaatgaatca gcgccgtgcc cgcgagccag
     2821 ggcatgaagg atgcgttttc caccgggtcc cagaaccacc agccgcccca gccgagttcg
     2881 agatacgacc agaagctgcc gatcgtgatg ccggccgtga gaaacagcca ggcgacgagc
     2941 gtccagggtc gcgtccagcg cgcccaggcg gtgtccatgc gcccgccgat gagcgcggcg
     3001 atcgcgaagg cgaaggcgac cgaaaagccg acgtacccca tgtagagcat cggcggatga
     3061 atggcgagca gcgggctctg gagaatcggg tttagatcgg caccgctcgc gggcggcggc
     3121 aacgcgcgcg cgaaaggatt cgacagccag atcgtgaacg cgagaaagcc gatgccgatc
     3181 acgcccatca cggcaagcac gcggcccgca aacaccgccg gcaggcggcg gctgaacgcg
     3241 gcgacggcca caccccagac cgaaagaatg aaggtccaca gcagcagcga gcccgcatgc
     3301 gcgccccaca cgccggtgaa cttgtagagc agcggcacga gcgcgttgga attctccgcg
     3361 acgtattcga ccgtgaggtc ggtgtgcacc atcgcccagg taagacatcc gaaggcggtc
     3421 gctacggcga gaaactgtcc gcaggcggcc gggcgcacga gcgcgaggag gcgcggcgaa
     3481 cgccgcgagg cgcccgcaaa tccgctcacc gcctgcgcca cggcaagcgc gagcgcgagc
     3541 atcagggcga actggccgag ttcaggaacc acggccgccc gcgcccgcgt ccatgctccc
     3601 cggtttgcga tacaccgtgc cgttctcctt gttgtagagc ttgagcggca tgtatttcga
     3661 atcgtgcttc gcgagcacgt cgttggcgac gaacacgccg ccgggcccca tgtgcccgtt
     3721 cgcgatgaca ccctgccccg gccggaagag atcgggtagc acgccggtga aaatcaccgg
     3781 aatgctgcgg cggttgtcgg taacgacgaa gcgtacggtg agcgatccgg gttggcgttt
     3841 caccgatccc ttctcgacga gaccgccgag gcggaatacg cgtttcggtc cgaggttctt
     3901 ctcgagcgtc tgggtcggcg tgtagtagta gagcagattg ccgctgaagg ccttgagcgc
     3961 gacgccgaca ccgatcgccg tgcccgcaag aatgccgacg acgacaagaa gccgctggcg
     4021 ccgcggcgtc atgcctcgtc ctcgaggtga cggatcgccc gttcccgcgc gcgccttagc
     4081 ccgcgcagcg caagccaggc gctcccggcc atcaccacga aggcagccgc gtaggcgggc
     4141 cacacgtaca gcgcgtagcc gcccatcgtc agaaattcgt gcaggcttct catcggacga
     4201 tcacctcagc atccgctgcg ccatcccgcg ggagcggcgg ttgcggcgca gaatctcgct
     4261 tcgcaagcga agtgtgatca accatgcaga atagcacgta aagcccacga tcatcacgag
     4321 cagcggccac agcatcgagg gcgcgatggc cgagccgctg agcgaaagac tcgcgccctg
     4381 atggagcgtg gtccaccatt ggaccgagta gtggatgatc ggcacgtcca ccgcgccgac
     4441 gatcgcgaga atggcgcccg cgcgatcccc ggtgcgttga tccgaaaatg cgttcgcgag
     4501 cgcgatgtag ccgagataga tgaagaacag gatcaattcc gaggtgagcc gcgcgtccca
     4561 cacccagtag gtgccccaca tcggcttgcc ccagagcgag cccgtgagga gcgcgagaaa
     4621 ggtgaaggcg gcaccgatcg gcgccgccgc tgccgtgatc gcgtgcgcca tgcgcagccg
     4681 ccacacgagc cccgcgatgc tcgcaagcgc catgatcgcg aacagcaaca tcgaaagcca
     4741 ggcgctcggc acgtgaatgt agatgattcg aaacccatcg ccctgctgat aatccggggg
     4801 cgcgagcacc aggccgccga tgagtccggc cacgatcagg gcgaggccga tccattgaaa
     4861 ccagggcgcc gtgcgcgcgg ccagccggaa gagcaccggc ggagatccaa gtttgtgaaa
     4921 ccaaacgaac acgacttcta agatctcccc gccgaggtgg gttgcgatac gatcattttc
     4981 tcaactctca tggctgatcc gcagcgccgc cgcggccgcc ggcggcaaca gactcagggc
     5041 gagaatgagg agcgccgcga gcgcggcgaa ggcctgcccg agcgggagtc ccgccatcgc
     5101 gagcgtcgcg ctgtgcgcac cgaaaatcag gaccggcacc gccagtggca gaatcaaaac
     5161 cggcgcgagc accgcggcct gacgcagcga cacggtgaga gcggcgacga gcgtaccgag
     5221 aagcgtcaac accggcgtgc cgagaagaag gccggcgacg agcgccgcga tccctcgtcc
     5281 cggtacgtac agcatcacgg caacaagcgc cgcgccgcag acgatcggaa gtccggtaag
     5341 aatccagtgt cccgaggcct tggcgagaat caccagactc agcggccttg gcgtcagcag
     5401 caactgctcc agcgttccgt cttcgtaatc cacgcgaaaa aacccttgca ggctcagcat
     5461 ggcagcgaga aatgcgatca cccagatcac cccggccgcg agcggacgca acaggtcagg
     5521 tgccgcgctc agtcccagcg gaaagagcat gctcgtcacc agaaaaa
//
LOCUS       DASUJG010000223         2208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_66047, whole genome shotgun sequence.
ACCESSION   DASUJG010000223 DASUJG010000000
VERSION     DASUJG010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66047"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /gene="lnt"
                     /locus_tag="VFX38_04760"
     CDS             <1..1159
                     /gene="lnt"
                     /locus_tag="VFX38_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX5314206.1"
                     /translation="WIPAAWILLEWVRSFLLTGFPWLALGYSQVATPLGAYAPILGVY
                     GAGFACMLVAFLLLVVVAPRSGLLARSAGVAAIIAVFAIGFGLGRIAWTHPGGPAFRV
                     SLVQGNIAQTLKWEPDMFARSVTRYRDLTAAHWSSALIVWPEDAVPLWYEQAEPALDA
                     LAREARRHGSEVVLGVPVYDEANDAGYNAVVSLGSVHATYYKRHLVPFGEYFPVPDWI
                     KSWLSAHALPYSSFTPGPWMQPPLAVGRWQAVVANCYEIAFGRLLITQLPAGEFILNL
                     SDDGWFGNSIALPQQFQMARLAARATGRYVLAATDDGITGIIDERGSVLATLPTHTVG
                     VLTGAARPFAGATPYVRMGNRGIVIACAFLVALALALALAAVLRRKSAADS"
     gene            1182..2162
                     /locus_tag="VFX38_04765"
     CDS             1182..2162
                     /locus_tag="VFX38_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019024385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEX5314207.1"
                     /translation="MNEPLLVVTHPACLQHDAGAAHPESPARLRAVLEAVESLALDHE
                     IEMAEAPRASREALLRVHDSAYLDELERRFPEEGRVQLDPDTIVSAGSREAAERAAGA
                     VEYAVAQVLAGAAKRAFCAVRPPGHHAEADRAMGFCLYNNVAVGAAAALADGLSRVAI
                     VDFDVHHGNGTEAMFKNDKRLLYCSLFQHPLYPGCGLDPTGNDVFVPLAAGTDGAAYR
                     AIFAERVEPALAGFRPELIFISAGFDAHAGDPLAGLALEDDDYAWLTERIVAAAEAHA
                     RGRVISVLEGGYHLAALRSATLAHLRALAASTPENGRTRPGHVPSSFQAL"
BASE COUNT          340 a          765 c          695 g          408 t
ORIGIN      
        1 ctggattccc gcggcctgga tactgctcga atgggtccgc agttttcttc ttaccggatt
       61 cccctggctc gcgctcggct acagtcaggt tgcgactccg ctcggagcat acgcgcccat
      121 tctcggcgtt tatggtgcgg gtttcgcctg catgctggtc gcgtttctcc ttctcgtcgt
      181 cgtcgcgccg cgcagcggtc tgcttgcgcg cagcgccgga gtcgccgcga tcattgcggt
      241 tttcgcaatc ggcttcggcc tcggacgcat cgcctggacg catcccgggg gccccgcgtt
      301 tcgcgtgagc ctggtgcagg gaaacattgc gcagacgctc aaatgggaac ccgacatgtt
      361 cgcccgctcg gtcacgcgct accgcgacct gaccgccgca cactggtcga gcgcattgat
      421 cgtgtggccc gaagacgcgg tgccgctctg gtacgagcag gccgagccgg ctctcgatgc
      481 gctcgcgcgc gaagcacgcc gccacgggag cgaagtggtg ctcggcgttc ccgtgtacga
      541 cgaagcgaac gatgccggct acaacgccgt cgtatcgctg ggcagcgttc atgcgaccta
      601 ttacaagcgc catctcgtgc ccttcggcga atattttccg gtgccggact ggatcaagag
      661 ctggttgagc gcgcatgcgc tgccgtattc cagtttcacg ccgggaccgt ggatgcagcc
      721 gccgctcgcc gtcggccgct ggcaggcggt cgtcgcgaac tgctacgaaa tcgcgttcgg
      781 acggctgctg attacgcaac ttcccgccgg cgaattcatc ctcaatcttt ccgacgacgg
      841 ctggttcggc aattcgatcg cgctgccgca gcagttccag atggcgcgcc tcgccgcgcg
      901 cgcgaccggg cgctacgtgc ttgctgcgac cgacgacggc attaccggca tcatcgacga
      961 gcgcggctcc gtgctcgcga ccctgccgac gcacacggtg ggcgttctca ccggcgccgc
     1021 tcgccccttc gccggcgcga cgccctatgt gcggatggga aaccggggaa tcgtgatcgc
     1081 ctgcgccttt ctcgtcgctc ttgcgctcgc gctcgcgctc gctgccgtgc tgcgacgaaa
     1141 gtccgcggcg gattcgtgat cgaatgcgag cggctgcaag gatgaacgag ccgcttctcg
     1201 tcgtgaccca tccggcctgc ctgcaacacg atgcgggagc ggcgcatccg gaatcgcccg
     1261 cgcgtttgcg agcggtgctg gaggcagtcg aatcgttggc gctcgaccac gagatcgaaa
     1321 tggccgaagc gccgcgagcg agccgcgaag cgctgctccg cgttcacgac tccgcgtatc
     1381 tcgacgaact cgaacgccga ttccccgaag agggcagggt gcagctcgat cccgacacca
     1441 tcgtttccgc cggaagccgc gaagcggccg agcgcgccgc cggcgccgtc gaatacgccg
     1501 tcgcgcaggt gcttgccggg gcggcgaagc gtgcgttctg cgcggtgcgc cctcccggcc
     1561 atcacgccga agccgatcgc gcgatgggat tctgcctcta caacaacgtt gccgtcggcg
     1621 cggcggcggc actcgccgac ggactgtcgc gggtcgcaat cgtggacttc gacgtccatc
     1681 acggcaacgg caccgaggcg atgttcaaga acgacaaacg cctgctgtac tgttcgcttt
     1741 tccagcatcc gctgtatccg ggttgcgggc tcgatccgac cggcaacgat gttttcgttc
     1801 cgctggccgc cggcaccgac ggggccgcgt atcgtgcgat tttcgcggaa cgggtggaac
     1861 cggcgctcgc gggcttccgc cccgaattga ttttcatttc cgccggcttc gacgcccacg
     1921 ccggcgatcc gctcgcggga ctcgccctcg aagacgacga ctacgcctgg ctcaccgaac
     1981 gcatcgtcgc cgccgccgaa gcgcacgcgc gaggtcgcgt catttccgtt ctcgaaggcg
     2041 gctaccatct cgccgccctg cgctccgcca cgcttgccca tctccgcgcc cttgccgctt
     2101 ccacgccgga aaatggaaga acaagaccag ggcacgttcc aagctcattt caagcgctat
     2161 aatcggctcc acatggctgc gaagctgtgt gcaacgcatc cagaaaat
//
LOCUS       DASUJG010000224         3579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_311672, whole genome shotgun sequence.
ACCESSION   DASUJG010000224 DASUJG010000000
VERSION     DASUJG010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311672"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /gene="pmbA"
                     /locus_tag="VFX38_04770"
     CDS             complement(<1..1221)
                     /gene="pmbA"
                     /locus_tag="VFX38_04770"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF008268.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEX5314208.1"
                     /translation="MTKPSAFFDSVALRESAGIALAAARERGASAAEVLVAVDEGLAA
                     TVRMGEIESVEHRRDRSFGVTLYFGLRKGSASTSDFSAPAIESSVAAAADIARHTAED
                     PCAGLAPAARMACEFPDLDLDHPWNVGADDAITLARECEDAARGADSRIVNSEGATLE
                     TGRGLSVYANSHGFVGERAGTRHDLSCVVVAANASGMQRDYWYTSARASADLESPGSV
                     GREAARRALARLEAGRIATRKARVLYAPEVARSLVGHLLGAISGGAQFHRASFLLEAA
                     GERVMPEWMNLVERPLLLRGAGSRAFDDEGVATAERALIEQGVLAGYLLGSYSARRLG
                     LETTGNAGGISNVIVQPGGELPDEPLAALGTGFYLTELIGQGVNGVTGDYSRGAAGFW
                     VENGQIAHPLEEVTV"
     gene            1288..2031
                     /gene="gpmA"
                     /locus_tag="VFX38_04775"
     CDS             1288..2031
                     /gene="gpmA"
                     /locus_tag="VFX38_04775"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEX5314209.1"
                     /translation="MPEVVLLRHGQSVWNLENRFTGWTDVDLSEAGIGEARAAGRLLA
                     EGGFDFDRCYTSVLTRAIRTLWLVLEELDRRWLPVTKDWRLNERHYGALQGKNKAETA
                     RAYSPEQVQEWRRSYDVRPPLLDASDPRHPRHDPRYRALDPAALPAAESLADTIARFV
                     PYYESEIARALASGERVLIVAHGNSLRALVKHLDRLSEQEITRLNIPTGIPLVYRLDD
                     SLRAISHRYLGDAAAAETAASAVADQAVL"
     gene            2028..2528
                     /gene="purE"
                     /locus_tag="VFX38_04780"
     CDS             2028..2528
                     /gene="purE"
                     /locus_tag="VFX38_04780"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX5314210.1"
                     /translation="MSHEAQVGVVMGSRSDWATLAEAASVLERLEIAHEVRVVSAHRT
                     PDLLFEYASGAAARGLRLIIAGAGGAAHLPGMLAAKTRLPVLGVPVKSRSLSGLDSLL
                     SIVQMPAGIPVASFAIGTAGAANAALFAAAVLALEDAGLAARLEAWRAEQTRAVRDDP
                     DPRRPR"
     gene            2525..>3579
                     /locus_tag="VFX38_04785"
     CDS             2525..>3579
                     /locus_tag="VFX38_04785"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEX5314211.1"
                     /translation="MKIGVLGAGQLARMLALAGYPLGARFRFLGRGREAPAGPLGELV
                     IGDFSDPAKIAELAQGADVLTFDVENIPADAVRAAGAERLCRPGLLALETGQDRLAEK
                     SCFEALGIPVAPWRRVDSRADLDAAVADLGLPFMLKARRLGYDGRGQRLIRKPDEAPA
                     AFAALGARALIAEAFVAFDREVSMLGVRAADGTTAFYPLCENQHTDGILRVSRAPAAD
                     AELSRLARDYVGRLLARLEYVGVLAVEFFLCDGRLLANEMAPRVHNSGHWTIEGTVCS
                     QFENHLRAVLGLPLGLTTALGNTAMVNLIGEMPDAAALLAIPGLHLHSYGKDPRPGRK
                     LGHATLVADDPQQLAGPL"
BASE COUNT          560 a         1252 c         1172 g          595 t
ORIGIN      
        1 gaccgttact tcctcgagcg ggtgcgcgat ctgaccgttc tcgacccaga atccggcggc
       61 gccgcgcgag tagtccccgg tcacgccgtt cactccctgc ccgatcagtt cggtgagata
      121 gaagccggtt ccgagtgcgg cgagcggttc gtccggaagt tcgccgcccg gttggacgat
      181 gacgttcgaa atgccgcccg cgttacccgt cgtttcgagc ccgagacgcc gcgcggagta
      241 gctgcccaga agatatccgg cgaggacacc ctgctcgatc agcgcgcgct cggcggtggc
      301 gacgccctcg tcgtcgaagg cgcgactgcc ggcgccccgg agcagaagcg ggcgctccac
      361 gaggttcatc cattccggca ttacccgctc gccggcggct tcgagcagga aggaggcgcg
      421 atgaaactgc gcgccgcccg aaatcgcccc gagcagatgg ccgacgaggc tcctcgccac
      481 ctccggcgcg tagagcaccc gcgccttgcg ggtcgcgatc cgcccggctt cgagccttgc
      541 aagtgcacgt ctcgcagcct cgcggccgac ggagcccggc gactcgagat ccgcggacgc
      601 gcgcgcgctc gtgtaccagt agtcgcgctg catgcccgag gcattggcag cgacgaccac
      661 gcaggaaaga tcatgacgcg tgcctgcgcg ctcgccaaca aagccgtgac tgttcgcgta
      721 gacggaaagg ccgcgaccgg tttcgagcgt cgccccttcg gaattgacga tgcgcgaatc
      781 ggcgcctcgt gccgcgtcct cgcattcgcg ggcgagcgtg atggcatcat cggcgccgac
      841 attccaggga tgatcgagat cgagatcggg aaactcgcag gccatgcgtg cggcgggagc
      901 gagtcccgcg caagggtcct cggccgtgtg gcgcgcgatg tcggcggcgg cggcaacgct
      961 cgattcgatc gccggcgcgg agaaatccga agtgctcgcg ctccccttgc gcaagccgaa
     1021 atagagcgtc accccgaagc ttcgatcgcg tcgatgctcg acactttcga tctctcccat
     1081 gcgcacggtc gccgcaagac cctcgtcgac ggcgaccagc acttccgcgg cgctcgcccc
     1141 gcgctcgcgg gcggcggcga gcgcgatccc ggccgactcg cgcagcgcca cggaatcaaa
     1201 aaaagcggaa ggcttcgtca ttttcgcaat tgtagccgca gacccgtttt tgctctaaaa
     1261 tgcgtcttta gaacccgctc gccggcaatg cccgaagtcg tccttctgcg ccacggccag
     1321 agcgtatgga atctcgaaaa ccgtttcacc ggctggaccg acgtggacct ctcggaggcg
     1381 ggaatcggcg aggcccgcgc cgccggacgc ctgctcgccg agggcggttt cgacttcgat
     1441 cgctgctaca cctcggttct gacgcgtgcg atccgtaccc tctggctcgt actcgaagag
     1501 ctggatcggc ggtggctgcc cgttaccaag gattggcgtc tgaacgagcg gcactacgga
     1561 gcactgcaag gaaaaaacaa ggccgaaacg gcccgcgcct acagccccga gcaggtacag
     1621 gaatggcggc gcagctacga cgtgcgtccc ccgctgctcg acgcatcgga tccgcgccat
     1681 ccgcgccacg atccgcgcta tcgtgcgctc gatccggcgg cgcttcctgc cgcggaatcg
     1741 ctcgccgaca ccatcgcgcg tttcgtcccc tattacgaaa gtgaaatcgc gcgggcgctc
     1801 gcgagcggcg aacgggttct gatcgtcgcg catggcaaca gcctgcgcgc cctggtcaaa
     1861 catctcgatc gcctttccga acaagaaatt acccggctga acatcccgac gggaattccg
     1921 ctcgtctacc ggctggacga ttcgctccgt gcgatctctc atcgttatct cggcgatgcc
     1981 gcggcggccg aaacggccgc aagcgcggtc gccgatcagg ccgtgttgtg agtcacgaag
     2041 cgcaagtcgg cgtggtcatg ggttcgcgct cggattgggc gacgctcgct gaagcggcga
     2101 gcgttctcga acgcctcgag attgctcacg aggtgcgggt cgtttcggcg caccgcacgc
     2161 cggatctgct cttcgaatac gcaagcggtg ccgccgcgcg cggcctgcgg ctcatcatcg
     2221 ccggcgccgg cggagccgcg catcttcccg gcatgctcgc tgccaagact cgccttcccg
     2281 tgctcggcgt gccggtgaaa tcgcgctcgc tttcgggact cgactcgctc ctctcgatcg
     2341 tgcagatgcc ggcgggaata ccggtcgcta gtttcgccat cggcacggcg ggcgctgcca
     2401 atgccgcact ctttgccgcc gccgtgcttg ctctcgagga cgccgggctc gcggcgcgtc
     2461 tcgaggcctg gcgagccgag cagacgcgag cggtgcgcga cgatccggac ccgcgccgac
     2521 cgcgatgaag atcggcgtac tgggcgcagg gcagctcgcc cggatgctgg ccctcgccgg
     2581 gtatccgctc ggtgcccgct ttcgcttcct cggacgtggc cgcgaggcgc ccgcgggtcc
     2641 tctcggcgag cttgtgatcg gcgacttcag cgatccggcg aagatcgccg agcttgcgca
     2701 gggcgccgat gtgctgacct tcgatgtcga aaacatcccg gccgacgcgg tgcgcgccgc
     2761 cggggccgag cggctctgcc gtccggggct tctcgcgctc gaaaccggtc aggatcgcct
     2821 cgccgagaag tcctgcttcg aggctctcgg catcccggtc gcgccctggc gccgggtaga
     2881 ttcgcgggcc gatctcgacg ccgctgtcgc cgatctcggg cttccgttca tgctcaaggc
     2941 gcgcaggctc ggctacgacg gccgcggcca gcgcctgatt cgcaagcccg acgaggcccc
     3001 cgccgcgttc gccgcactcg gggcgcgggc gctcatcgcc gaagccttcg tcgccttcga
     3061 tcgcgaggtc tcgatgctcg gcgttcgcgc cgcggacgga acgacggcgt tctacccgct
     3121 ttgcgagaat caacacaccg acggcatcct gcgggtctct cgcgctccgg cggcagacgc
     3181 ggaactctcc cgcctggctc gagactacgt cgggcgcctg ctcgcccgcc tcgagtacgt
     3241 tggcgtgctc gcggttgaat ttttcctgtg cgacggacgc ctgctcgcaa acgaaatggc
     3301 gccgcgagtc cacaattcgg gacactggac gatcgagggc acggtttgca gccagttcga
     3361 aaaccatctg cgtgcggtgc tggggctgcc gctggggctg accactgcgc tcggcaacac
     3421 cgcgatggtg aatctcatcg gggagatgcc cgacgcggcc gcgctgctcg caatccccgg
     3481 gctgcatctg cattcctatg ggaaggaccc gcggccgggg cgaaaactcg gccacgcaac
     3541 cctcgtcgcc gacgatccgc agcaactcgc cggaccgct
//
LOCUS       DASUJG010000225         3514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_311739, whole genome shotgun sequence.
ACCESSION   DASUJG010000225 DASUJG010000000
VERSION     DASUJG010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311739"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="VFX38_04790"
     CDS             complement(<1..899)
                     /locus_tag="VFX38_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX5314212.1"
                     /translation="MATFAHDMPWGAQIEDGCVRFRLWAPRQHAPQLILESEGGERNF
                     SMQECGGGWFELRSAAARAGSRYCYALDEGLRVPDPASRFQADDVHGASMVVDPRAHE
                     WREPPWRGRPWRETVLYEAQVGAFGASGDYDGLRRRLDTLVDLGITALELMPIAHFEG
                     RRGWGYDGVLLFAPQPAYGSVTQLKRLVDAAHERELMMFLDVVYNHFGPSGNYLGHYA
                     PEFFTDRYCTPWGEAIDFSRREVRDFYLQNALYWLEEYRFDGLRLDAVDHIHDASAKH
                     ILVELAETVRERIRDREVHLVLE"
     gene            complement(967..3105)
                     /gene="glgX"
                     /locus_tag="VFX38_04795"
     CDS             complement(967..3105)
                     /gene="glgX"
                     /locus_tag="VFX38_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEX5314213.1"
                     /translation="MSMPRSKPRVLPGSPYPFGATWDGRGVNFALFSAHAEKVELCIF
                     ESNGARERERIALPERTDQVWHGYLPHAHPGTLYGYRVYGPYDPKRGHRFNHHKLLLD
                     PYACLLQGRLRWSDAQFGYRVGSPREDLSFDRRDNARGMWKCEVVDPAFSWGGDRLPA
                     RPWDETVIYEAHLRGLTMRHPAIEERFRGTDAGLASPAMIGHLKSLGITAIELLPVQA
                     FVNDRYLVQKNLSNYWGYNTIAFFAPKPRYLARGKLFNFKTMVARLHDAGIEVILDVV
                     YNHTAEGNRLGPTLCFRGIDNASYYRLQADDPRYYTDDTGTGNTFNLGHPRVLQMVMD
                     SLRYWAGEMHVDGFRFDLASALARGPEGFDPHCSFLDALRQDPLLATRKLIAEPWDLG
                     PGGYQLGHYGHGWSEWNDRYRDTVRRFWRGDAGAVPELATRIMGSADLFDHDGRRPRA
                     SLNFVTAHDGFTLADLVSYEHKHNEANGEDNRDGTDNNLSANYGAEGASDDEKILELR
                     RRQRRNFLATLLLSQGTPMILAGDEFGHSQGGNNNAYCQDNEMSWLDWEAIDDRARAD
                     VDFLRHLLHLRRQHPALRWPHFLHGRAQCDAGIKDIAWIAPDGTEMSEEHWRNPEARA
                     VGVLLNGNAGKPAAERAQPPDEILLILLNAGEEECAFTLPRLPVGSGWVRVLDTTAQS
                     AAAVSLHEFEETFALAGRAVMLFHLTVHGE"
BASE COUNT          651 a         1100 c         1133 g          630 t
ORIGIN      
        1 ttttccagca ccagatgcac ttcgcgatcg cgaattcgct cgcggacggt ctcggcaagc
       61 tcgacgagaa tgtgtttcgc gctcgcatcg tgaatgtggt ccacggcatc gaggcgcagg
      121 ccgtcgaagc gatattcctc gagccagtaa agcgcatttt gaagataaaa gtcgcgcact
      181 tcgcggcggg agaaatcgat ggcctctccc cagggcgtgc agtatcggtc ggtaaagaac
      241 tccggcgcgt agtgcccgag atagttcccg ctcggcccga agtggttgta gaccacgtcg
      301 agaaacatca tgagttcgcg ttcgtgcgcc gcatcgacga gccgcttcaa ctgcgtgacg
      361 ctgccgtacg ccggctgcgg cgcgaacagg agcacgccgt cgtaacccca gccgcgtcgt
      421 ccttcgaaat gcgcgatcgg catcaactcg agcgccgtga tgccgagatc gacgagagtg
      481 tcgagccggc ggcgcaggcc gtcatagtcg ccgctcgcac cgaaggctcc gacctgcgcc
      541 tcgtagagca cggtctcgcg ccagggacga ccgcgccacg gcggctcgcg ccattcatgg
      601 gctcgcggat cgaccaccat gctggcgccg tgcacgtcgt cggcctggaa gcgcgaggcc
      661 gggtccggaa cgcgaagccc ctcgtcgagc gcgtagcaat agcggctgcc cgcccgcgcc
      721 gcagcgctgc gcagttcgaa ccagcccccg ccgcattcct gcatcgagaa gtttctttcg
      781 ccgccttcgg attcgaggat caattgcggc gcgtgctgcc gcggcgccca taaacgaaat
      841 cgcacgcagc cgtcttcgat ctgcgcgccc caaggcatgt catgcgcgaa ggttgccatc
      901 agtccggcga tacccctgtc ggcatccgca tcgtttccgc gttgcaggga aacgctgtgg
      961 acggagtcac tccccatgca ccgtcagatg aaacagcatc accgcgcgcc cggcaagggc
     1021 gaaggtttcc tcgaattcgt gcagcgagac ggccgcggcg gattgcgcgg tggtgtcgag
     1081 cacgcgaacc cagccgctgc cgacgggaag acgcggcagc gtgaaggcac attcctcttc
     1141 gccggcgttc agcaggatca gaagaatttc gtcggggggt tgcgcgcgct ccgccgcggg
     1201 ctttccggcg ttgccgttga gaagcacgcc gacggcgcgg gcctcgggat tgcgccaatg
     1261 ttcctcggac atctcggttc cgtcgggcgc gatccaggcg atgtccttga tgcccgcgtc
     1321 gcattgcgcc cggccgtgga gaaaatgcgg ccagcgcagc gccggatgct gccggcgcag
     1381 atgcagcaga tgacgcagga agtccacatc ggcgcgcgcg cgatcgtcga tcgcttccca
     1441 gtcgagccag ctcatttcgt tgtcctggca gtaggcattg ttgttgccgc cctgactgtg
     1501 gccgaattcg tcgccggcca ggatcatcgg cgttccctgc gacaacagca gcgtcgcgag
     1561 gaaattgcgc cgctggcgtc ggcgcagttc gagaatcttc tcgtcgtcgc tcgcgccctc
     1621 cgctccgtaa ttggcgctca gattgttgtc ggtgccgtcg cgattgtcct cgccgttggc
     1681 ctcgttgtgc ttgtgttcgt aggaaaccag atcggccagc gtgaagccgt cgtgggcggt
     1741 gacgaaattg aggctcgcgc gcgggcggcg cccgtcgtga tcgaagagat ccgccgagcc
     1801 catgatgcgg gtcgcaagtt cgggaacggc tcccgcgtcg ccgcgccaga agcggcgcac
     1861 ggtatcgcga tagcgatcgt tccattccga ccagccgtgt ccgtagtggc cgagctgata
     1921 gccgccggga ccgagatccc agggctccgc gatcagtttt ctcgtcgcga gcagcggatc
     1981 ctggcgcagt gcgtcgagaa agctgcaatg cggatcgaag ccttcgggtc cccgggcgag
     2041 cgcgctcgcg agatcgaaac ggaagccgtc cacgtgcatc tcgcccgccc agtagcgcag
     2101 gctgtccatg accatctgca gcacgcgcgg gtggccgaga ttgaaggtat tgccggtgcc
     2161 ggtgtcgtcg gtgtaataac ggggatcgtc cgcttgcagg cgatagtagc tcgcgttgtc
     2221 aatgccgcgg aagcacagcg ttggcccgag gcgattgccc tcggcggtgt gattgtaaac
     2281 gacgtccaga atcacttcga tgcccgcgtc gtgcagccgc gcgaccatgg tcttgaagtt
     2341 gaagagtttt cctcgcgcga gataacgagg tttgggtgcg aaaaacgcga tggtgttgta
     2401 accccagtaa ttgctcaggt tcttttgcac cagatagcgg tcgttgacga aggcctgcac
     2461 cggcaacagt tcgatcgcgg tgatgccgag gcttttgaga tgtccgatca tggcgggaga
     2521 cgccagcccg gcatcggtac ctcgaaagcg ctcttctatc gccggatggc gcatggtgag
     2581 ccctcgcaaa tgcgcctcgt agatgaccgt ctcgtcccac ggacgtgcgg gcaggcgatc
     2641 gcctccccaa ctgaaggccg ggtccacgac ctcgcacttc cacatgccgc gggcgttgtc
     2701 gcgccgatcg aaggagagat cctcgcgcgg cgatcccacc cgatacccga attgcgcgtc
     2761 ggaccatcgg agcctgccct gcaggaggca ggcgtaggga tcgagaagca atttgtgatg
     2821 attgaagcgg tggccgcgct tgggatcgta cgggccgtac acgcggtagc cgtacagcgt
     2881 gcccggatgc gcgtggggaa gataaccgtg ccagacctgg tcggtgcgtt ccggaagcgc
     2941 gatacgctcg cgctcgcgcg cgccgttcga ctcgaaaatg cacagttcga ctttttccgc
     3001 gtgggccgaa aagagcgcga agttgacgcc gcggccatcc caggtggcgc cgaacggata
     3061 gggacttccc ggcaaaaccc gcggcttgga ccggggcatg gacatctcct cttggttacg
     3121 aattgcacca acacgacatc cgcgccgacg gaggatcgtc tcggcaaatc gtctccgaac
     3181 atcttagccg aaggaggggc tcagaagccg tcgtcatgag aacgcatacg ttctgcgcaa
     3241 tcggcatgcc tgcagggcac gtgcgcaagc gcacggaacg cttgccacga cttgattatg
     3301 ctgccaatga agatcggaaa ctttgatcgt ctcgacaagt gggacgagct ctttcgatct
     3361 tcgctatcca gcaaccccaa gaggtgaagc catgacgaac caggatcaat cccgcgacaa
     3421 gccggatccg tcccgcggca ggcccgatca gtcccaagac aaagacaagg accggggcaa
     3481 gcacggtcaa tccggggacc ggccggatca atcc
//
LOCUS       DASUJG010000226         4635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_312430, whole genome shotgun sequence.
ACCESSION   DASUJG010000226 DASUJG010000000
VERSION     DASUJG010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312430"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1955
                     /locus_tag="VFX38_04800"
     CDS             <1..1955
                     /locus_tag="VFX38_04800"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314214.1"
                     /translation="DDKFAAGFEVTNLTSTKREVETLIRAEGNAVNGQPSAHDRFALA
                     SYAHRPFWLNLAAPAPGTIRLTATATAGTLGDAMAATIPVRRAHTPITAAEYGSTTGK
                     KAVVPVKIPPQAIPGATTLSVTLAPTLVGGLANAFVALRDDPIESWEIRLSRAVLASD
                     YLRLKPVLGDSVKWGDAQSAITKSLQAATDFQAPGGGMAFWIPRDDFASPYLSVYTAL
                     AFDWLARAGHPVAPTVEARLRAYLRKHVLALDDASSPAAPVLRAAALAALAPAGKLPA
                     GAVAGMIPELPKLRLFGQALLLEAALEVKDEKSAATIAQSILSHAEESAGEISFNERI
                     PGAYLDLLASPLRSNCAALDALVQYAAAHKGGGLVGTTPQKLLRWVIAARAQSGAWPN
                     SQENVFCTTATLHYAALYETPVKRLGATVAAADQTLGKAEFASQSTPARTMRAPAPAT
                     SGNFSVTVAPSGAGRLYYNVNLTYALPPASLGPADAGFTLHREYQVERSGKWQPVTAD
                     TTLARGYVLRVDLDLDVPTERHHVVLDDPLPGAFEAVNRNLATAMTSTPDAQPDNLVL
                     WFDSGAWPNGTVTTSGFYHRETGFDAVRFFADNLMPGNYHLIYAVQVISPGRFIAPAA
                     TVREIYQPDIFGRGSSQVIQVAPPQQ"
     gene            1952..4177
                     /locus_tag="VFX38_04805"
     CDS             1952..4177
                     /locus_tag="VFX38_04805"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX5314215.1"
                     /translation="MKRRYWIGGGLALLAAVALALALFTRAALMPLPAALPPPSSTPA
                     LLFTARDGTPLNRTHRSRFNYTQRLPLWRMPVLLKEAFVASEDRRFWQHGGADWRARF
                     AALGDDLRARRIVRGASTIGEQAARILEPRPRNYWSHWIAGFDADRLIERFGRADVLQ
                     FYLNQVPYGGRRRGVLPAAHYYFGRDLAALSPAELLALAILVRSPESYDPRRHSQHLR
                     AAVDELAARMRRESAIGAMQYAAIVRSPIVPGHEALALNAGPFVRYAAKRAQALGLPG
                     IRQRTTLDPALERFVQAALRNRLAQLADRGAKNAAALVVDNATGEILAWAVAPGGNAF
                     GIDPVLAPRQPGSALKPFVYALAMEQLGWQADTLIDDAPLAEKVGSGVHRYRNYSDRY
                     YGEVSLRYALANSLNIPAVETAQAVGTANILALFERLGFRGFEHTADYYGPAIALGDG
                     AVRLFDLVQAYAALARRGRFLPLRVLAAAPAPPSSSVVSPAVTSLIADILSDPSARAA
                     EFGANSVLDLPWPTAVKTGTSSDYRDAWAVGFDNRYTIGVWMGRLAGGRMQQVTGSLG
                     PALVLRQIFDRLRPEGAYAGLWRSPKLQAITACEPIGPERCTMRPEWRLPGEMTATQP
                     HAAIAIAEPLPGETLALDPRLPAAAQRYRFRISGGDGDIAAVVWRLDGKTLTTTRAPS
                     ASWQLVAGVHRLSAEVRLTTGIAGLRLGQVQFRVLGGEAATKDSRSTATGSVSPPGDG
                     G"
     gene            complement(4373..4459)
                     /locus_tag="VFX38_04810"
     tRNA            complement(4373..4459)
                     /locus_tag="VFX38_04810"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4423..4425),aa:Leu,seq:taa)
BASE COUNT          759 a         1702 c         1440 g          734 t
ORIGIN      
        1 tcgacgacaa attcgccgcc ggcttcgagg tcaccaacct cacgagtacg aaacgcgaag
       61 tcgaaaccct gatccgcgcg gaaggcaatg cggtgaacgg acaaccgagc gcgcacgacc
      121 gcttcgcgct cgcctcctac gcgcaccgtc ccttctggct gaatctcgcg gctccggctc
      181 ccggcacgat ccgacttacc gctaccgcga cggctggaac gctcggcgac gctatggcgg
      241 caacgatccc ggttcgtcgc gcgcataccc cgatcaccgc cgccgaatac ggcagcacga
      301 cgggaaagaa ggccgtcgtg cccgtgaaga ttccgccgca ggcgatcccc ggcgcgacga
      361 cgctttcggt gacactcgcg ccgacgctcg tcggcggact cgccaatgcc ttcgtcgcgc
      421 tacgcgacga tccgatcgaa agctgggaga ttcgcctgag ccgcgccgtg ctcgcgagcg
      481 attatctgcg cctcaagccc gttctcggcg acagcgtgaa atggggcgat gcgcaaagtg
      541 ccatcaccaa gtcgctccag gccgcaaccg actttcaggc ccccggcggc ggcatggcct
      601 tctggattcc gcgcgacgac ttcgcgagcc cctacctttc ggtttatacc gcgctcgcgt
      661 tcgactggct cgcgcgcgcc ggccatcccg ttgcgccgac ggtcgaggct cggttgcgcg
      721 cgtatctgcg caagcatgtg ctcgcgctcg acgatgccag ctccccggcg gcgccggtgt
      781 tgcgcgcggc tgcgctcgcc gcgctcgccc ctgccggcaa acttcccgcg ggcgccgtcg
      841 ccggcatgat tcccgaactg ccgaagctgc gcctcttcgg tcaggcgctg ctgctcgaag
      901 cggcgctcga agtcaaagac gaaaagagcg ccgcgaccat cgcgcaatcc attctttccc
      961 atgcggaaga atccgccggc gagatctcct tcaacgaacg catccccggc gcctatctcg
     1021 atctgctcgc gagcccgctg cgctcgaact gcgcggcact cgatgctctc gtgcaatacg
     1081 cggcagcgca caagggaggc ggcctcgtcg gcacgacgcc gcagaaactg ctccgctggg
     1141 tgattgccgc gcgggcgcaa agcggcgcat ggccgaacag tcaggaaaac gtgttctgca
     1201 cgaccgcgac cctgcactat gcggcactct acgagactcc ggtgaaacgg ctcggcgcaa
     1261 ccgtcgcggc cgcggaccaa acgctcggaa aggcggaatt tgcctcgcaa agcactcccg
     1321 cgcgaacgat gcgcgcgccg gcgcccgcga cgagcggaaa cttttccgtc acggtcgccc
     1381 cgagcggcgc aggccggctc tactacaacg tcaacctgac gtacgcattg ccgcccgcct
     1441 cgctcgggcc ggcggatgcg ggcttcacgc tgcaccgtga atatcaggtc gagcgctccg
     1501 gcaaatggca gccggtcacg gcggacacga ccctcgcccg cggctacgtc ctgcgcgtgg
     1561 atctcgatct cgacgtgccg accgaacgcc atcacgtcgt cctcgacgat cccctccccg
     1621 gcgccttcga ggccgtcaac cgcaatctcg cgacggcgat gacgagtacg cccgacgcac
     1681 aaccggacaa tctcgtgctc tggttcgaca gcggtgcctg gccgaacggc accgtcacca
     1741 ccagcggctt ttatcatcgc gaaacgggct tcgatgccgt gcgcttcttc gccgacaacc
     1801 tgatgcccgg caactatcat ctgatctacg ccgtgcaggt catcagtccc gggcgcttca
     1861 tcgcgccggc ggcgacggtg cgcgaaatct atcagcccga catattcggc cgcggcagct
     1921 cgcaagtcat ccaggtcgcg cctccgcaac aatgaagcga cgctattgga tcggcggcgg
     1981 cctcgcgctc ctcgcggccg tggcgcttgc gctcgcgctg ttcacgcgcg ccgccctgat
     2041 gccgcttccc gcggcgttgc cgcccccttc ctccacgccc gcgctgctct tcaccgcccg
     2101 cgacggcacg ccgctcaacc gcacgcatcg cagccgtttc aactacacgc aaaggcttcc
     2161 gctgtggcgg atgccggtgc ttctgaagga ggctttcgtc gcttccgaag accggcgctt
     2221 ctggcaacac ggaggcgccg actggcgcgc gcgcttcgcg gcgctcggcg acgatcttcg
     2281 cgcgcggaga atcgtccgcg gcgcgagcac gatcggcgag caggcggcgc gcatcctcga
     2341 accccgcccg cgaaactact ggagccactg gatcgccggc ttcgacgccg atcgcctgat
     2401 cgagcgcttc ggccgcgccg acgtattgca gttctacctc aaccaagtgc cgtacggcgg
     2461 ccggcgccgc ggcgtgctgc cggcggcaca ctactacttc gggcgcgatc tcgccgcgct
     2521 ttcgccggcg gagcttctgg cgctcgcgat cctcgtgcgc tcccccgaat cctacgatcc
     2581 gcgacgtcat tcgcaacatc tgcgcgccgc cgtcgacgaa ctcgcggcac ggatgcgcag
     2641 ggagtccgcg atcggcgcca tgcaatacgc cgccatcgtc cgctcgccga tcgtccccgg
     2701 acacgaggcg cttgccttga acgcgggccc gttcgtgcgc tacgccgcga agcgcgcgca
     2761 ggcactcggc ctgccgggaa tccgccagcg cacgacgctc gatccggcgc tcgaacgctt
     2821 cgtgcaagcc gcgctgcgca accgcctcgc gcaactcgcc gatcgcggcg cgaaaaatgc
     2881 ggcggcgctg gtcgtggaca atgcgacggg ggagattctc gcctgggccg ttgcccccgg
     2941 aggcaatgcg ttcggcatcg atccggtact cgctccgcgc cagccgggct ccgcgctcaa
     3001 acccttcgtc tatgcgctcg cgatggagca actcggctgg caggccgaca ccctcatcga
     3061 tgatgcgccg cttgccgaga aggtcggctc cggtgtgcat cgctatcgca actacagcga
     3121 tcgttactac ggcgaagtca gcctgcggta cgcgctcgcg aattcgctca acattccggc
     3181 ggtggaaacg gcacaggccg tcggcaccgc gaacatcctc gccctcttcg agcgactcgg
     3241 tttccgcggt ttcgagcaca cggccgacta ttacggcccc gcgatcgcgc tcggcgacgg
     3301 cgccgtgcga ctcttcgatc tcgtgcaggc gtacgcggcg ctcgcccgtc gcggacgctt
     3361 tctgccgctg cgcgtactcg ccgccgcgcc ggcaccgccg tcctcctccg tcgtcagtcc
     3421 ggcggtcacg tcgctcatcg ccgacatcct ctccgatccc tcggcccgcg ccgcggaatt
     3481 cggagcgaac agcgtgctcg accttccctg gccgaccgcc gtgaaaaccg gcacttcgag
     3541 cgactatcgc gacgcctggg ccgtcggctt cgacaatcgc tacaccatcg gcgtatggat
     3601 gggaagactc gccggcggac gcatgcagca ggtgacgggc tcgctcggcc cggcgctggt
     3661 gctccgccag atattcgatc gtctgcgccc cgagggcgct tacgccggac tgtggcggag
     3721 ccccaaattg caggcgatca cggcctgcga accgatcggc ccggagcgct gcacgatgcg
     3781 ccccgaatgg cgtctgcccg gcgagatgac ggcaacgcag ccccatgccg caatcgcgat
     3841 cgccgagccg cttcccggcg aaacgctcgc actcgatccg cgcctgcctg ccgctgcgca
     3901 gcgttaccgc tttcgcatct ccggcggcga tggtgacatc gccgccgtcg tgtggcggct
     3961 cgacggaaag accttgacca caacccgcgc gccgtccgcg agctggcaat tggtcgccgg
     4021 agtgcaccgc ctcagcgccg aggttcgact caccacggga atcgccgggc ttcgcctcgg
     4081 tcaggtgcaa tttcgcgtcc tcggcggcga agccgcgacg aaagattccc gctcaactgc
     4141 gaccggcagc gtttcaccgc ccggagacgg aggatgaccg actggcagac acagcggacg
     4201 tgtggacagg catgacgtta gccgtcggcc taacgagggg cacaagctcg cgcgcaagtc
     4261 ctacgaccat gccatcggcg aggagatcat caggaaacct ccccgcgccg cgagaaaagg
     4321 cccggctcgt cttggctgtc aagacaccgg cacccgccgg gcggggcggc gttggtgcca
     4381 ggggccggag tcgaaccggc atggcccgaa ggccagcgga ttttaagtcc gcagcgtcta
     4441 ccgatttcgc caccctggcg cgcctgcgtc gctcgatccg gtcgagacgt cagcatcggc
     4501 agcacgaggg accgatgcgt agggatcgcg ctcatgttga tccaatcgtg acgggcacgt
     4561 caacgcccgt cgcttgcgga tcacaacacc catggaccgc gaaccgaaac ggttcgcgaa
     4621 ggttctcggc atgga
//
LOCUS       DASUJG010000227         2629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_67094, whole genome shotgun sequence.
ACCESSION   DASUJG010000227 DASUJG010000000
VERSION     DASUJG010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67094"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VFX38_04815"
     CDS             complement(<1..386)
                     /locus_tag="VFX38_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314216.1"
                     /translation="MGPPEALAGALGYSEFLQRVFSGLDRGAGVDLLRAARAPLCRAN
                     AAAVLDRAPSLVQGIEQTLAWIALRELTGLACVRESLLAASLLATLTLRRAFAPEPRD
                     GVPDLVVFALGKLGGRELNFYSDLDLV"
     gene            512..1183
                     /locus_tag="VFX38_04820"
     CDS             512..1183
                     /locus_tag="VFX38_04820"
                     /inference="COORDINATES: protein motif:HMM:NF003974.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HEX5314217.1"
                     /translation="MFLTLPDVLEPAAVSALREAVATAQWEDGLATTGGAGRGRKHNE
                     QVAAGESRLAAWQELVLRALRRHPMMQFLVLPKRFMAPVFNRYEPGMEYRDHVDFPLL
                     GGGDTRMRADLSLTLFLSAPESYEGGELVIEAGSGERRIKLPAGQAYVYPASTLHRVE
                     PVRSGSRLAAVTTIQSHIRGESERALLADMLTLWRRIEDLAPASDEQRLAAKIHHNLV
                     RMWAD"
     gene            complement(1285..2208)
                     /locus_tag="VFX38_04825"
     CDS             complement(1285..2208)
                     /locus_tag="VFX38_04825"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5314218.1"
                     /translation="MEDRSHALIAIIFLVVFAVGALLVVWWMLTPGAVRVPYVLESKT
                     SVAGVGEGTAVQFNGIKIGVVKSIKIDPATHRRIQVRIVVDKKFPLPKGSYATVGSSS
                     LVGPTIVDIHLGDSSQIIQTSAQHPAHLSVKQGGLSAMMDEAGEIVAKAKQTLDSVQK
                     IVSDKNVKQISDTLKNIHEASGQLVVLEKDIAPGAKQLPQLIAETRGVMKQAQRLVAN
                     ANGLVVSARAPLSAVGEAASSAAAFATDLDQQTAPQLNALLVKLRGLTTQLDGLAEEL
                     RQTPQSLILGPAKPQPGPGETPPPAAPPGGG"
     gene            complement(2225..>2629)
                     /locus_tag="VFX38_04830"
     CDS             complement(2225..>2629)
                     /locus_tag="VFX38_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5314219.1"
                     /translation="LQMSGLSADDANKRPAELSGGMVKRAALARALAVEAELLFLDEP
                     GSGLDPETARDLDGVLGDLHKELNLSIVMVSHDLDSVAALADRVAILAEKRILFEGTF
                     TELGDSEALEHAVVKRLLEGPRGASVRARMKH"
BASE COUNT          452 a          876 c          841 g          460 t
ORIGIN      
        1 acgagatcga gatcggaata gaagttgagc tctctgccac cgagcttgcc gagcgcgaag
       61 accaccagat ccggtacgcc gtcgcgcggt tcgggcgcga acgcacggcg cagcgtgagc
      121 gtggccagaa ggcttgcggc caacaggctc tcgcgaacgc aggcaagacc ggtaagttcg
      181 cgcaaggcga tccatgcaag cgtctgctcg atgccttgca cgagcgacgg ggcgcgatcg
      241 agcaccgccg ccgcattcgc gcgacacagc ggagcgcgtg cggcgcgaag caagtcgaca
      301 cccgccccac gatcgagccc ggagaagacc cgttgcagaa attcgctgta cccgagtgct
      361 ccggcgagcg cctcgggtgg ccccaactcc tcggtcgcga acccgaggcg ggcgaggcgt
      421 tcccgcccgc gcgcgccgag ttccctcagg gcgtccgtgc ggagcccccc cgcggcgggt
      481 aaaatcgatt tcgtcctgcc ggaaaattcc gatgttcctc acgcttcccg acgttctgga
      541 gccggctgcg gtcagcgcat tgcgcgaggc cgtcgccacg gcgcaatggg aggacggcct
      601 cgccaccacg ggcggcgccg ggcgcgggcg caagcacaac gagcaggtgg cggccgggga
      661 atcgcgactc gcggcctggc aggaattggt gctgcgcgcg ttgcggcgcc atccgatgat
      721 gcagtttctg gtgcttccca aacgcttcat ggcgccggtg ttcaaccgct acgaacccgg
      781 aatggagtac cgcgatcacg tggactttcc cctgctcgga ggcggtgaca cgcgcatgcg
      841 cgcggatctc tcgctcaccc tgtttctctc cgcgcccgaa agctacgagg gcggcgaact
      901 cgtgatcgaa gccgggagcg gcgagcggcg catcaagctc cccgccggcc aggcgtacgt
      961 ctatccggcc tcgaccctgc atcgggtcga gccggtgcgc tcgggttcgc gcctcgccgc
     1021 cgtcaccacg atccagagcc atattcgcgg cgaaagcgaa cgtgccctgc tcgcggatat
     1081 gctcactctg tggcggcgca tcgaggatct cgcgccggca agcgacgagc agcgcctcgc
     1141 cgcgaaaatt catcacaacc tcgtgcggat gtgggccgac tagagtcgta acgcgacggc
     1201 gattcgtcag cgcgtgcgac gaaccgctcg aagacgttgg ctcagacatc gcgccttcgc
     1261 aatgagaagg tgccggaacg ccctttatcc cccgccgggg ggcgcggccg gtggcggcgt
     1321 ttcgcccggt ccgggctgtg gcttcgccgg cccgagaatg aggctttgcg gagtctgccg
     1381 cagctcttcg gccaggccgt cgagttgggt ggtcaggcca cgcaacttca cgagcagcgc
     1441 gttgagttgc ggcgcagtct gctgatcgag atcggtcgcg aaggccgcgg cggaactcgc
     1501 ggcctccccg actgcggaaa gcggcgcgcg ggcggaaacg accagaccgt tggcgttcgc
     1561 taccagtcgc tgtgcctgct tcatcacccc gcgcgtctcg gcaatgagtt gcggcagctg
     1621 tttcgcgccg ggcgcgatgt ccttttccag gacaaccagt tgcccgctcg cctcgtgaat
     1681 gtttttcaag gtatccgata tctgttttac attcttgtcg gagacgattt tctgcaccga
     1741 atcgagcgtt tgcttcgcct tggcgacgat ctcccccgcc tcgtccatca tggcgctgag
     1801 gccgccttgc ttgaccgaca agtgtgccgg gtgctgcgca gaggtttgaa tgatctgcga
     1861 gctgtcgccg agatgaatgt ccacgatcgt gggtccgacc agactgctcg atcccacggt
     1921 ggcataactg cctttcggca gcggaaattt tttgtccacg acgatccgca cctggatcct
     1981 gcggtgcgtg gccggatcga tctttatgct cttgactacg ccgatcttta tcccgttgaa
     2041 ttgcacggcg gtaccctcgc cgactcccgc gacgctcgtt ttggactcaa gcacataagg
     2101 cacccgcacc gcgccgggcg tgagcatcca ccacacgacc aacagggcac cgacggcaaa
     2161 cacgaccaga aatatgatgg caatcagggc atgagagcgg tcttccatcg cggtgctccg
     2221 tgacttagtg tttcatgcgc gcgcgcaccg aggcgccgcg cggaccctcg agcaaccgct
     2281 tgacgacggc atgctccagc gcttccgaat cgcccaattc cgtgaacgtg ccctcgaaca
     2341 ggatacgctt ttcggccagt atcgcgaccc ggtcggcaag ggccgccacc gaatcgagat
     2401 cgtggctgac catcacgatg gaaaggttca gctccttgtg cagatcgccg agaaccccgt
     2461 ccagatcgcg cgcggtttcc ggatcgagcc ccgagcccgg ttcgtcgagg aagagaagct
     2521 cggcctcgac cgccagagcg cgggcgagcg ccgcccgttt caccatgccg ccggaaagtt
     2581 ccgccggccg tttgttggca tcgtccgccg agagcccgct catctgcag
//
LOCUS       DASUJG010000228         2823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_313126, whole genome shotgun sequence.
ACCESSION   DASUJG010000228 DASUJG010000000
VERSION     DASUJG010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313126"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="VFX38_04835"
     CDS             complement(<1..490)
                     /locus_tag="VFX38_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5314220.1"
                     /translation="MNLRDLRYLEALAEHGHFGRAAKASFVSQPTLSMQIRKLEKELG
                     VVLVERAPRKLIFTETGNAVLAEAREILAHVERIRALARQARDPAARSLRLGIFPTLG
                     PYLLPHVLPAIRKRFPHLELLLTEEKTEEVLARLREGRLDAVIVALPVHEEGLHVEFL
                     FKE"
     gene            616..1155
                     /locus_tag="VFX38_04840"
     CDS             616..1155
                     /locus_tag="VFX38_04840"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020459891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX5314221.1"
                     /translation="MLTVGDRFPSFKLKATVGIDKPETAFREISDETHRGEWQLVFFY
                     PKDFTFVCPTEIKGFADLNKQFEDRDCRILAASTDSEFVHLAWRRDHKDLRELPFPML
                     ADINKDLARALGILDEEGVARRATFLVDPEGVIRFVYVTEGSVGRNPAEVLRVLDALQ
                     TDELCPCNWHEGEETLKAA"
     gene            1231..1770
                     /locus_tag="VFX38_04845"
     CDS             1231..1770
                     /locus_tag="VFX38_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX5314222.1"
                     /translation="MTLEQLKDQLPGYAKDLRLNLESVLGETGAAGLTQKQIALVALA
                     SAIASRYAPLAAAASKFAAEHASAEELDGARAAAAIMAMNNVYYRFTHSLGDAEYATL
                     RAGLRMNVIARPGCDRTSFELACLAVSAINGCAACMDSHEKTLRSQGLGAQGVQSAAR
                     IAAVVHAVAVTLEQASPMG"
     gene            1879..2238
                     /locus_tag="VFX38_04850"
     CDS             1879..2238
                     /locus_tag="VFX38_04850"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX5314223.1"
                     /translation="MNRIIRSGLVAAACAAGAIAVGACSHNSQTPAGQISNGAQEMGQ
                     GIAGVASDSTITTKVKAKMAANTGLSSFSIHVSTTNGIVTLTGTVDTDATRDLAEHVA
                     ATTGGVKSVNNELKVGG"
     gene            complement(2317..2457)
                     /locus_tag="VFX38_04855"
     CDS             complement(2317..2457)
                     /locus_tag="VFX38_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314224.1"
                     /translation="MEVFAGYALGGDLMLAAAIVAAFFAIKAERRSLESVAAPLGLCP
                     DP"
     gene            complement(2593..>2823)
                     /locus_tag="VFX38_04860"
     CDS             complement(2593..>2823)
                     /locus_tag="VFX38_04860"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017842039.1"
                     /note="catalyzes the formation of
                     pyridine-2,3-dicarboxylate and 5-phospho-alpha-D-ribose
                     1-diphosphate from nictinate D-ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase"
                     /protein_id="HEX5314225.1"
                     /translation="EALAAGAERILLDNFDLHGLREAVRVNGRRAKLEASGNVDFDRL
                     RAIAATGVDFISVGALTKHLHALDLSLRFDAV"
BASE COUNT          547 a          929 c          908 g          439 t
ORIGIN      
        1 gctccttgaa cagaaactcg acgtgcagcc cttcttcgtg cacgggcagg gcgacgatca
       61 cggcatcgag ccgcccttcc cgcaatcggg cgagcacctc ttcggttttc tcctcggtca
      121 ggagcagctc gagatgcggg aaacgtttgc ggatcgccgg cagcacgtgc ggtagcagat
      181 acgggcccag ggtcgggaaa attccgaggc gcaggctgcg ggccgcggga tcacgggcct
      241 gccgcgcaag cgcgcgaatg cgctcgacat gagcgagaat ctcgcgggct tcggcgagca
      301 ccgcgttgcc ggtctccgtg aagatcagct tgcgcggagc gcgctcgacg agaacgactc
      361 cgagttcctt ctccaatttg cgaatctgca tggatagggt gggctggctg acaaagctcg
      421 ccttggcggc acggccgaag tggccgtgtt cggcgagggc ctcgagatag cgaaggtcac
      481 gcaggttcat ggcagtaata gttaatagct atcaatagta tataaataat atatttgatt
      541 tatcaacccc gcgcccctat attgaattcg aaggcggcgc aacgcgccgc gcccaacccg
      601 aacggagatt gcatcatgtt gaccgtagga gacagattcc cgagcttcaa actcaaggcg
      661 accgtcggca tcgacaagcc ggagacggcc tttcgcgaga tcagcgacga gacacatcgc
      721 ggcgaatggc agctcgtttt cttttacccg aaggacttca ccttcgtctg cccgaccgag
      781 atcaagggct tcgcggatct gaacaaacag ttcgaggacc gcgactgccg catcctcgcg
      841 gcgagtaccg acagcgagtt cgtgcatctc gcctggcggc gcgatcacaa ggatctgcgc
      901 gagctgccct ttccgatgct cgcggacatc aacaaggacc tcgcccgcgc gctcggcatc
      961 ctcgacgagg aaggggtcgc ccgccgcgcc acgttcctcg tggatcccga gggcgtgatc
     1021 cgcttcgtgt atgtcaccga gggcagcgtc gggcgcaatc cggcggaggt gctgcgcgtg
     1081 cttgacgcct tgcagaccga cgagctgtgt ccctgcaact ggcacgaagg cgaagaaacg
     1141 ctcaaggccg cctgaacacc tacggctttt ccctctcccc cttcgcttgc gaagggggat
     1201 cgagggggat gcctctctca ggagaatccg atgaccctcg aacaactgaa ggatcaactg
     1261 cccggctacg ccaaggatct gcgcctgaac ctcgagtccg tgctcggcga gaccggggca
     1321 gcgggactca cgcaaaagca gattgccctg gtggcactgg cctcggcaat cgcctcccgc
     1381 tacgcgccgc tcgccgcggc cgcaagcaag ttcgcggccg agcatgcgag cgcggaagaa
     1441 ctggacggcg cgcgggcggc cgctgcgatc atggccatga acaacgtgta ctaccgtttc
     1501 acccattcgc tcggcgatgc cgaatacgcg acgctgcgcg cggggctgcg catgaacgtc
     1561 atcgcgaggc cgggctgcga ccgcaccagc ttcgaactcg cctgtctcgc cgtttcggca
     1621 atcaacggct gcgccgcctg catggattcc cacgagaaga cactccgctc gcagggcctc
     1681 ggcgcccagg gggtgcagag cgcggcccgc atcgcggcgg tggtccacgc cgtggccgtc
     1741 acgctggaac aagcttcccc tatgggctga tccgcggcgt gccgccccgg ttgccacccc
     1801 ggaatcccgt acaatcatgg tcgaatcccg agccctcgca agggtccggg cccggcacaa
     1861 acgaaggaga tctctcaggt gaacagaatc atccgcagcg gcctggttgc cgccgcctgc
     1921 gccgccggcg cgatcgcggt cggcgcctgc agccacaaca gccagacacc cgcggggcag
     1981 atcagcaacg gtgcccagga aatgggccag ggaatcgcgg gcgtcgcgag cgacagcacg
     2041 atcacgacca aggtgaaggc gaagatggcg gcgaacacgg ggctgagcag cttcagcatc
     2101 catgtttcca cgaccaacgg gatcgtcacc ctgacgggca cggtggatac cgatgcgacc
     2161 cgcgatctcg ccgaacatgt cgccgcgacc accggcgggg tcaagagcgt caacaacgaa
     2221 ctcaaggtcg gcggctgagc ccgggccggc gttgcgttgc cgttcggcat tgtgccggcg
     2281 acagccgatc accctgcaag gagcggagac gcgccatcac ggatcggggc acaggccaag
     2341 cggcgcggcg accgattcga gtgagcggcg ctcggccttg atggcgaaaa acgccgccac
     2401 gattgccgcg gcgagcatca ggtcgccgcc gagcgcgtag cccgcgaaca cttccacgcg
     2461 cgcccgcgag gcgatcagaa ggaccgaagc gccgcgatca gccgcgtgtg aaacgcgacc
     2521 agcgcaaccg ctcgagccgt gcgggaatac cggtgcgaat gccggcaacg gcactgcgct
     2581 ccctgtgccg gcctacacgg catcgaaacg cagcgaaagg tccagcgcgt gcaggtgttt
     2641 ggtgagcgcg ccgacggaga tgaagtccac ccccgttgcc gcgatcgcgc gcagcctgtc
     2701 gaagtccacg ttgcccgaag cttccagctt cgctcgccgc ccgttgacgc gtaccgcctc
     2761 gcgcagcccg tggagatcga agttgtcgag caatatccgt tcggcgccgg cggcaagcgc
     2821 ttc
//
LOCUS       DASUJG010000229         2417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_556329, whole genome shotgun sequence.
ACCESSION   DASUJG010000229 DASUJG010000000
VERSION     DASUJG010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556329"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VFX38_04865"
     CDS             <1..199
                     /locus_tag="VFX38_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314226.1"
                     /translation="LTVIMVTHDLTSVRRLADRAILLHEGSILADGTPGELLQSDDPQ
                     VRYFFQLEVKKSRLVPAMEHL"
     gene            201..1112
                     /locus_tag="VFX38_04870"
     CDS             201..1112
                     /locus_tag="VFX38_04870"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5314227.1"
                     /translation="MGHRVSYSVIGAFVILLGGALVVGLLWLASGGLARNYTTYVIYL
                     KSGAASLSADSAVLYNGVAVGQVSSIQLDPKNPSQALILLKIREGTPVKTDTVAMVET
                     RGVTGSGYVELNGGKPTAPLLEAQPGQKYAVIPTKPGTLASLANAAQSAAGRIGQIAK
                     RLDAILSDKNVNAISESLNNIREVTANFAAQSRALDSALANLDATLANTRKATADLPA
                     LVQHLHATILSFQQLSDNARTALATVNRTASGFGQLTPQAQALLTRLNQATENLNALL
                     QMLNANPSSLVFGKASKPGPGESKGGG"
     gene            1115..1762
                     /locus_tag="VFX38_04875"
     CDS             1115..1762
                     /locus_tag="VFX38_04875"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HEX5314228.1"
                     /translation="MRKLFALTLPLLLVAGCSGLFTPKSLPTSEYALAPTVASAAVPA
                     RSCAAVLRVNSVQVAQPWGSSNILYTQTPYQVSSFAYHRWAAPPATMLGDAITKALAA
                     SELYRGVLGPTDPGNGDLTLAIRLLRGPVQSFPGSQSGAAAPAASTEALGFAASLSVS
                     DTGRLLASREFSASTAAAPNPYGGVVAANKLAGDLIARLIGWLASVDTASVCATP"
     gene            1845..>2417
                     /locus_tag="VFX38_04880"
     CDS             1845..>2417
                     /locus_tag="VFX38_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5314229.1"
                     /translation="MVTATIEREATLRRETLDAYFARVRAATLALVAGLAPEDSVVQS
                     MDDASPVKWHLAHTTWFFETFVLAPFARNYRRFHEGYDFLFNSYYQLVGPMYRRADRG
                     LLSRPTLAEILAYRAHVEEAVANLLREDDAGEIALRVTLGCEHEQQHQELIATDLKHA
                     FSHNPLAPAWRELPPPGTRAPPPAGWRKFDG"
BASE COUNT          413 a          839 c          750 g          415 t
ORIGIN      
        1 gctcacggtc atcatggtga cgcacgatct cacctcggtc cggcggctcg ccgaccgtgc
       61 tattctcctg catgaaggca gcatcctcgc cgacggcacg ccgggtgaat tgcttcagag
      121 cgacgatccg caggtgcggt atttcttcca gctcgaggtg aagaagagcc ggctcgtgcc
      181 ggccatggag catctctgac atgggtcatc gggtcagtta ttccgtcatc ggcgccttcg
      241 tcatcctgct cggcggcgca ctcgtcgtcg gactgctgtg gctcgcctcg ggcggactcg
      301 cgcgcaacta cacgacttac gtgatttatc tcaaatccgg cgcggcatcg ctttccgccg
      361 actcggccgt gctctacaac ggcgtcgcgg tcgggcaggt atcctcgatc cagctcgatc
      421 cgaaaaatcc cagccaggcc ctcattttgc tgaagatccg cgagggcacg ccggtcaaga
      481 ccgacacggt tgccatggtg gagacgcgcg gggtcaccgg ctccggctac gtggaactca
      541 acggcggcaa gcccacggcg ccgctgctcg aggcgcaacc ggggcagaaa tatgcggtga
      601 ttccgacgaa gccgggcact cttgcctcgc tcgcgaacgc ggcccagagt gcggcggggc
      661 gcatcggcca gattgccaaa cgcctcgatg caatcctgag cgacaagaat gtgaacgcga
      721 tctcggagag cctgaacaac attcgcgagg tcaccgccaa cttcgccgcg cagtcgcgcg
      781 cgctcgattc cgcgctcgcc aatctcgatg cgacgctcgc caatacccgc aaggcgacgg
      841 ccgatctgcc ggcgctggtg caacatctgc acgcgacgat cctgagtttt cagcaactgt
      901 cggacaacgc tcgaaccgcg cttgcgaccg tcaatcgcac ggcgagcggt ttcggacaac
      961 tgacgccgca ggcgcaggct ttgctcacga gactcaatca ggcgaccgaa aacctgaacg
     1021 ccctgttgca gatgctcaat gccaatccga gttcgctggt gttcggcaag gccagcaagc
     1081 ccggccccgg agaatccaaa ggaggaggct agacatgcgc aagctcttcg cactcacgct
     1141 gccgctgttg ctcgtcgccg gatgcagcgg acttttcact cccaagtcgc tgccgacgtc
     1201 cgagtacgcg ctcgcgccga cggtggcgag cgccgccgtt cccgcgcgct cctgcgcggc
     1261 ggtactgcgc gtcaactcgg tgcaggtggc gcagccctgg gggagcagca acattcttta
     1321 cacgcagact ccctaccagg tgtcgagctt tgcctatcac cgctgggcgg cgccgccggc
     1381 gacgatgctc ggcgatgcga tcaccaaggc gctcgcggcg agcgaactgt atcgcggagt
     1441 actcgggccg accgatccgg gcaacggcga tctcaccctt gccattcgcc tcctgcgcgg
     1501 tccggtgcaa agtttccctg gctcgcaaag cggtgccgcg gcgcccgccg cgagcaccga
     1561 agcgctcggc ttcgccgcct cgctcagcgt gagcgatacg ggtcggttgc ttgccagccg
     1621 cgaattctcg gcttcgacgg ctgccgctcc caatccctac ggcggagtcg tcgcggcgaa
     1681 caagcttgcg ggcgatctca tcgcccggct gatcggctgg ctggcgtccg tcgataccgc
     1741 gagcgtctgc gccacaccct gagccgatcg ccgccggctc gacgagacgg cgaggcccga
     1801 agccgccttt gacatcgcgc cgggcgggcg gctaggcttc tcccatggtc acagccacga
     1861 tcgagcggga agccaccctg cgccgggaga cgctcgacgc gtactttgcc cgcgtgcgcg
     1921 ccgcgacgct cgcgctcgtt gccggcctcg cgcccgaaga cagcgtggtg cagtcgatgg
     1981 acgatgcgag tcccgtcaag tggcatctcg cccacaccac ctggttcttc gaaaccttcg
     2041 tgctcgcgcc gttcgcaaga aattatcggc gcttccacga gggctacgat tttctcttca
     2101 acagctacta tcaactcgtc ggaccgatgt atcggcgcgc cgaccggggc ctgctttcgc
     2161 gcccgacgct cgccgaaata ctcgcctatc gcgcgcacgt tgaggaagcc gtcgcgaatc
     2221 ttctgcgcga ggacgatgcc ggcgagatcg cgttgcgcgt caccctcggt tgcgagcacg
     2281 aacagcagca tcaggaactg atcgcgaccg atctcaagca tgccttctcg cacaacccgc
     2341 tggcgcccgc ctggcgcgaa ttgccgccgc cgggaacgcg ggcgccgccg cccgccggct
     2401 ggaggaagtt cgacggc
//
LOCUS       DASUJG010000230         3533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1413967, whole genome shotgun sequence.
ACCESSION   DASUJG010000230 DASUJG010000000
VERSION     DASUJG010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413967"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..653)
                     /locus_tag="VFX38_04885"
     CDS             complement(12..653)
                     /locus_tag="VFX38_04885"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEX5314230.1"
                     /translation="MTVADLRALDLRDLNVAGVGEWPLAGRLTLLGLVLLAVLAIGYF
                     GFVRGELANLKTARQQEVTLKLQFEKKQQQVAQLAAYQKQLAAMRKTFGAMLLKLPSR
                     SEIDSLLRDISQTAQEDGLAQKLFQPQGERRMDFYAEKPIVMEYVGSYQQVSTFVSDV
                     SSLPRIVTLENFSLAPVKKDEGSQLDFKVKAMTYRYLSESELDQGATKKGKKH"
     gene            complement(650..1234)
                     /locus_tag="VFX38_04890"
     CDS             complement(650..1234)
                     /locus_tag="VFX38_04890"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEX5314231.1"
                     /translation="MARINLLPWRQWRRRERRRQFVLMLVLALLAGIVIVFWTDRSIS
                     AAVATQQARNTYLQRQIARLDHQIETIRNLKKTRDALLARMRIIEQLERSRPTAVHLF
                     DQLVRTVPDGLYLTSVADDGGKLKIAGTAQSPAAVSTYMQRIAGSGWLDPPNLQVVRT
                     HTEGNDKYSDFSVTTSVSASAPSAQVATVAGAGP"
     gene            complement(1234..2346)
                     /gene="pilM"
                     /locus_tag="VFX38_04895"
     CDS             complement(1234..2346)
                     /gene="pilM"
                     /locus_tag="VFX38_04895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HEX5314232.1"
                     /translation="MHSRFEFAWFGRSWGSLVTVESRLKSRPVLGLDISATAVRVVEL
                     SRVGVSYRVESYAVEPLPADAIGEQHLADVETAGVAVRRAYQRCGSRLKSAAVAIDAA
                     NAIIRTLQLPAGLSSREQEEQVEVLAARHIPYPLEEVAWDYEVLGPSLQPPGSEDVLL
                     VAARRDHIEHRQAILEIAGLEARVMDCEVFALENAYGLVRGQIANGASVTAFANFGTA
                     STNFSFFDGKRIVYTREQAFGSRQLVDEIMRRYDLSYEDAVADLRQGDLPRDYREEVL
                     DPFVTDMALQTNRALQFFLASTSEHASVDQVVLCGSSAAIPGAAERIASQIGKPTVAA
                     NPLAELELGERVRAQGIERDAPALMVACGLALRSFD"
     gene            2417..>3533
                     /locus_tag="VFX38_04900"
     CDS             2417..>3533
                     /locus_tag="VFX38_04900"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX5314233.1"
                     /translation="MSRGRRVLGWLLIAVAALGLAAVLALVGVYFYISAGLPSVAVLK
                     DIHLQVPLRVYTRDGKLMAVYGDKLRIPLDYNQIPPLMSEAFIAAEDERFFEHPGLDV
                     RSLSRAGINLIVTGSKSQGASTITMQLARNFFLSPKKLYIRKLREIFLALHIERELSK
                     EDILTLYLNKIYLGNHAYGVGAAAEVYYGRDVWQLDLAQIAMIAGLPQAPSSHNPIVD
                     PQGALARRAYVLGRMRDLGYIDQSAYQRAMTAPLTARRHQARVTVQAPYVGEMVRSYM
                     VNHYGKSAYTAGYRVTTTIDSRLERDARAAVNWNLERLDREHGWRGAVSHVVLPADAD
                     AAALNQAIADFHPVENLIPGVVTGTDDQSADLFLGGYG"
BASE COUNT          620 a         1190 c         1095 g          628 t
ORIGIN      
        1 cggtgatgcg ctcaatgctt ctttcccttc ttggtcgcgc cctggtcgag ttccgattca
       61 ctcagatacc ggtaggtcat cgcctttacc ttgaaatcca gttgcgagcc ttcgtccttc
      121 ttcaccggtg cgagagaaaa gttctcgagc gtcacgatgc gcggcagcga cgagacgtca
      181 ctgacaaacg tcgagacctg ctgatagctg ccgacgtatt ccatgacgat cggcttttcc
      241 gcgtagaagt ccatgcgccg ctccccctgc ggctggaaaa gcttctgcgc gagcccgtct
      301 tcctgcgccg tctgcgagat gtcgcgcagc aggctgtcga tttcgctcct gctcggaagt
      361 ttcaacagca tggcgccgaa ggttttgcgc atggcggcga gctgcttctg atatgccgcg
      421 agttgggcga cctgttgctg ctttttctcg aattggagct tgagcgtcac ctcctgctgc
      481 cgggcggtct tgagattcgc cagctctccg cgcacgaatc cgaagtagcc gatcgcaaga
      541 acggcgagca gcaccaggcc gagaagcgtg agacggcccg ctagcggcca ttcgccgacg
      601 ccggcgacat tgagatcgcg caggtcgagc gcccgcaggt ccgctacggt cacggtccgg
      661 ctccggcgac ggtcgccacc tgcgcgctcg gcgccgaggc cgagacgctg gtcgtgaccg
      721 aaaaatcgct gtacttgtca ttgccctcgg tatgggtccg cacgacctga agattgggcg
      781 gatcgagcca gcccgagccc gcgatcctct gcatgtacgt ggacacggct gccggagact
      841 gtgcggtgcc ggcgatcttg agcttgccgc cgtcgtcggc aaccgaggtg agatagaggc
      901 cgtcggggac cgtgcgcacg agctggtcga acaggtgcac ggccgtcggg cgactgcgct
      961 cgagctgttc gatgatgcgc attcgggcaa gcaaggcgtc gcgcgtcttc ttcagattgc
     1021 ggatcgtttc gatctggtga tcgagcctcg cgatctgacg ctgcagatag gtgttgcgcg
     1081 cctgctgcgt cgccaccgcg gcggaaatgc tgcggtcggt ccagaagacg atcacgatcc
     1141 cggcgagcag cgcgagaacc agcatgagca caaactgccg gcgccgctcg cgccgcctcc
     1201 actggcgcca gggcaggaga ttgattcgcg ccatcagtcg aagctccgca gcgcgagtcc
     1261 gcaggcgacc atgagcgcgg gcgcatcgcg ttcgattccc tgggcccgca cccgctcccc
     1321 caactccagt tcggcgagcg ggttggccgc gacggtgggc ttgccgatct gcgaggctat
     1381 gcgctcggcg gcaccgggga tcgcggcgga actgccacag agaacgacct gatccacgct
     1441 cgcatgctcg ctcgtggagg cgagaaagaa ttgcagcgcc cggttggtct ggagcgccat
     1501 gtcggtcacg aaggggtcga gcacctcctc gcgatagtcg cgcggaagat ctccctgacg
     1561 cagatcggcg accgcgtctt cgtaggacag atcgtagcgc cgcatgattt cgtccacgag
     1621 ctggcgcgag ccgaaggcct gctcacgcgt gtagacgatc cgcttcccgt cgaagaaact
     1681 gaaattcgtg ctcgcggtac cgaaattcgc gaacgccgtg acgctcgcgc cgttcgcaat
     1741 ctggccgcgg accagcccat aggcgttttc gagtgcgaac acctcgcaat ccatcacgcg
     1801 ggcctcgagg ccggcgattt cgaggatcgc ctggcgatgc tcgatgtggt cgcgccgcgc
     1861 ggcaaccagc aacacgtctt ccgatcccgg cggctggagc gaaggcccga gcacctcgta
     1921 gtcccaggcg acttcctcca gcggatatgg aatgtggcgg gcggcgagca cctccacctg
     1981 ctcctcctgc tcgcgcgacg agagacccgc gggaagctgc aaggtgcgga tgatcgcgtt
     2041 cgcggcgtcg atcgcgacgg cggcgctctt gagacggctg ccgcaacgct gatacgcccg
     2101 gcgcaccgcg acaccagccg tttccacgtc ggcgagatgc tgttcgccga tggcatcggc
     2161 gggaaggggt tcgaccgcgt agctctcgac gcggtacgat acgccgaccc gggacagctc
     2221 gacgacgcgc accgcggttg cgcttatgtc gagtcccagg accggcctgg atttcagcct
     2281 gctctccacg gtcaccaagc tcccccacga ccgtccgaac cacgcaaact cgaaccgcga
     2341 gtgcactata gtacttttac ccgagatgtc aagcagcgtg agtcacaaca ccagccgtcc
     2401 ttccaagcct cgcaagatga gtcgcggccg ccgcgtgctc ggctggctgc tgatcgccgt
     2461 ggccgcgctc ggactcgccg ccgtgcttgc gctggtcggc gtctacttct acatctcggc
     2521 ggggctgcct tcggtcgccg ttctcaagga cattcacctc caggttccgc tgcgcgtcta
     2581 cacccgtgac ggcaagctca tggccgtcta cggagacaag ctgcgcattc cgctcgacta
     2641 caaccagatt ccgccgttga tgagcgaggc cttcatcgcc gccgaggacg agcgcttttt
     2701 cgagcacccc ggactcgacg ttcgcagcct cagtcgcgcc ggcatcaatc tcatcgtcac
     2761 cggcagcaag tcgcagggcg cgagcacgat cacgatgcag ctcgcgcgca atttcttcct
     2821 cagcccgaag aaactctaca tccgcaagct gcgcgagata tttctcgcgc tgcatatcga
     2881 gcgcgaactt tccaaggaag acatactcac gctctatctc aacaagatct acctcggcaa
     2941 tcacgcctac ggcgtcggtg cggccgccga ggtctactac ggcagggacg tctggcagct
     3001 cgacctcgcc cagatcgcga tgatcgcggg attgccccag gcgccctcct cgcacaaccc
     3061 catcgtggac ccgcagggcg cactcgcccg tcgcgcctac gtactcgggc gcatgcgcga
     3121 tctcggctac atcgatcaga gcgcctacca acgggcgatg acggcaccgc tcacggcccg
     3181 ccgtcaccag gcgcgggtga ccgtgcaggc gccctatgtc ggcgagatgg tgcgcagcta
     3241 catggtgaat cactacggca agagcgccta caccgccggc tatcgcgtca cgacgaccat
     3301 cgactcccgg ctggaacgag acgcacgcgc cgccgtcaac tggaatctcg aacgcctcga
     3361 tcgcgagcac ggctggcgcg gagcggtcag tcacgtggtg ctcccggccg atgccgacgc
     3421 cgccgccctc aatcaggcga ttgcggattt ccatccggtg gaaaacctca ttcccggagt
     3481 cgtcacgggc acggacgacc aatccgcgga cctcttcctc ggcggctacg gcg
//
LOCUS       DASUJG010000231         6852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_314509, whole genome shotgun sequence.
ACCESSION   DASUJG010000231 DASUJG010000000
VERSION     DASUJG010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6852
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314509"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /gene="rnk"
                     /locus_tag="VFX38_04905"
     CDS             complement(<1..328)
                     /gene="rnk"
                     /locus_tag="VFX38_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013466038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HEX5314234.1"
                     /translation="MDSKPHIVISSRDMERLEALLKVLPANTVPGRRDLQEELDRAEV
                     VAPEKVPHSVVTMNSTVRFVLESSGEEFSLTLVYPKDVKSDGGTISILAPVGSALLGL
                     SIGDSIE"
     gene            complement(513..1946)
                     /locus_tag="VFX38_04910"
     CDS             complement(513..1946)
                     /locus_tag="VFX38_04910"
                     /inference="COORDINATES: protein motif:HMM:NF015043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HEX5314235.1"
                     /translation="MDELVSIGLRDTEASWESTDPYLRDGFAPVHDECDASDLPVSGK
                     LPAELAGVYVRNGPNPAFAPISYTWPFDGDGMLHAVYLESGRARYRNRYVATPGLAAE
                     RRAGRAIYGGVAKPFPVAPSLVGPGGDPGPLKNGAFIHVVGHGGRMYAMWEGGTVREI
                     TRELETRGEWKPGMSAPFAVAPHTKLDPATGELFLINYALLPPCVQLYVLGPAGGLRR
                     RVDVKLPVATMMHDFVLTERYIVLFHFPLVLDPLAPAKREPFLQWRPELGTRIGLVAR
                     DDLAGGVRWIETDPFFAFHFANGFERDGALVVDYVHRPEFVLSRNRQPRASAPAMRRL
                     QIDLAAGTCRSDPISDAATEFPRINESFATRASRWVYAPVRRGPAAADGEAPVYRGIA
                     KFDCENGNTMVFDLGGDEIGEAVFVPRANASVEDDGWLMAYVYRPRERASDFCVFDAR
                     ALDAGPLASVRLPRRVPHGLHGNWFPA"
     gene            complement(2146..3678)
                     /locus_tag="VFX38_04915"
     CDS             complement(2146..3678)
                     /locus_tag="VFX38_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEX5314236.1"
                     /translation="MSGTAESAGSRRWILAGLMATMMLAAMDSTIVSTAIPQIVGDLG
                     DFSLFAWVFSIYVLAQTVTIPVYGKLADQFGRKPVLIVGTLVFLAGSAASAGAWSMLS
                     LIVFRAFQGLGAGSIMATVNTLAGDLFSIRERARVQGWLSSMWGLSAIIGPALGGAFA
                     EYASWRWIFLVNLPVGAVSLVLIGRNLHESFARSRHRVDFSGAALVLATVGMLVLGLL
                     QGGEQWPWLSLPSGIVFAFVLVFAALTIAVERRAAEPILPAWLWRRRVLAGSNLAMVG
                     MGIVMMGPIAYLPTFAQSVYGLGAIAAGFLLAAMSVGWPIASALSGRIYLRLGFRDSA
                     LGGGVLMVLAAVGFLALPDPGPVWAVVLDQVVLGAGFGLLSTPLLVGVQSVVGWEERG
                     VVTGTNMFARYLGQSLGAAIFGAIFNAALASQLAAAPAALRSRLPQQVNEVIHALHDP
                     ALGAAARGFLRHAIYVATHHIYWGIALLSILTLACVLVAPGRFPLVGGDGGPRRSANG
                     NT"
     gene            complement(3675..4466)
                     /locus_tag="VFX38_04920"
     CDS             complement(3675..4466)
                     /locus_tag="VFX38_04920"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX5314237.1"
                     /translation="MNILPARRNKYGGVEVDSGALPGDAAHFASLLAHSLEQWKAQGA
                     TMVWLSLARERAPLIAAALAAGFEFHDCSSEQLMLVCPLEESASVPPCATHTLGVGAV
                     VISEARELLTVLERYELESRPEYWKLPGGTLEPGEDIARGVVREVREETGIEARFEGI
                     VALRHHHHGQFATSDLYVICRLTPLSRAIVMDETELARARWMPLDDFLANDGVGLLNK
                     RAVELAMTHPCMKSAKIAGYMNGPNDYEIFLPEGAQEAAHTPAGK"
     gene            complement(4468..6219)
                     /locus_tag="VFX38_04925"
     CDS             complement(4468..6219)
                     /locus_tag="VFX38_04925"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX5314238.1"
                     /translation="MGLMKLDRTPRLLIMSVFLGLVGGLGAELFLLALHWANMYILGF
                     LGHYSTINVAVAHAAGRAPEPFTHWYWWVPLATTVGGLVVGFLIYTFCPEAEGHGTDS
                     AVRAFHVNRGRMRARIPLIKGIASAITIGSGGSAGREGPTAQIAAGVGAIASSWLKLP
                     DDERRIIILMGMAAGLSAIFKSPLGTAIFAVEVLYSGMAFEGGALIYTLISAAVAYAI
                     IGSFDGFTPLFVLSSDVGMGAAVNLVWFAILGLVAGVFGALLPTVFYRVRDGFRALAI
                     PDHFKPAIGGLAVGLIGIALPPLLGGGYGYIQFALQGGAGLAIWLLLILALGKILALA
                     LTVGSGGSGGVFAPALYVGAMLGATFAGLLGLFHLEISASALAVVGMAALFAGAARIP
                     IAALVMVIEMTGGYQLVLPTMIAVALAFVVQMALTRRARYPTLYEAQVPGPADSPVYR
                     EVYRHTMENLLRRHELKLGEELLDEEMQTALASGEGVTLDGRGERLYHLSLAAGAPLA
                     GRTIRSLGLDRMGVVIVGLIRGEREVLPRGSTRLLVGDGLLVAAGRDAIAAFRKRIAP
                     RASNAAQLKLTSVANAS"
     gene            6420..6496
                     /locus_tag="VFX38_04930"
     tRNA            6420..6496
                     /locus_tag="VFX38_04930"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6454..6456,aa:Ile,seq:cat)
     gene            6713..6852
                     /locus_tag="VFX38_04935"
                     /pseudo
     CDS             6713..6852
                     /locus_tag="VFX38_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983411.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
BASE COUNT         1349 a         2275 c         2229 g          999 t
ORIGIN      
        1 attcgatgct gtcgccgatc gagaggccga gcaacgcgct gccgaccggc gccagaatgg
       61 aaatcgtgcc accgtcgctc ttgacgtcct tcggataaac cagcgtcaac gaaaactcct
      121 cgcccgacga ttcgagaacg aagcgtaccg tggagttcat ggtaacgacc gaatgcggta
      181 ccttctccgg agccacgact tcggcccggt caagctcctc ctgcaaatcg cgcctgccgg
      241 gaacggtgtt tgccggcaac actttgagca gcgcctccaa cctttccatg tcgcgcgaag
      301 aaataacgat atgaggtttg gaatccatcg gtcgcccctt gcaagctctg gatcgacagg
      361 ctcaacttat atcacaactg tccggtatcc gcagcgacag ccgcggccgg cgcccgttcg
      421 ggatttccgc gtggcgatcg gattgcacat cgcgaactcg atgacgcgct cgctccgccc
      481 cggcccgcct acaacggcgg caagccacgc cgtcaggcgg ggaaccagtt gccgtgcagg
      541 ccgtggggaa cgcgccgggg caaacgcacg cttgcgagcg ggccggcgtc gagcgcgcgc
      601 gcatcgaaca cgcagaagtc actcgcccgc tcgcgcggac ggtacacgta ggccataagc
      661 cagccgtcgt cttcgacgct cgcgttcgcc cgtggcacga agaccgcttc gccgatttcg
      721 tcgccgccga gatcgaacac catcgtgttc ccattctcgc aatcgaattt cgcgatcccg
      781 cgatagaccg gcgcttcgcc atcggcggct gcgggaccgc ggcgcacggg tgcgtacacc
      841 caacgcgatg cgcgggtcgc gaagctctcg ttgatgcggg gaaactccgt ggcagcatcg
      901 gagatcggat cgctccggca ggtaccggcg gcgagatcga tttgcagccg ccgcatggcc
      961 ggtgcgctcg cacgcggctg ccgattgcga gaaagcacga attccggccg atgaacgtaa
     1021 tccacgacga gcgcgccgtc gcgttcgaat ccgttggcga agtggaatgc gaagaaggga
     1081 tcggtctcga tccagcgtac gccgccggcg agatcgtcgc gcgcgacgag tccgattcgc
     1141 gtgccgagtt ccgggcgcca ttgcagaaat ggctcgcgct tcgccggcgc gagcggatcg
     1201 agaaccagcg gaaaatgaaa cagcacaatg taccgttccg tcagcacgaa gtcgtgcatc
     1261 atcgtcgcca ccggcagttt cacgtcaacg cgccggcgca atccgcccgc cggacccagc
     1321 acgtagagtt gcacgcaggg cggcaggagc gcgtagttga tcaggaacag ctcgcccgtc
     1381 gccggatcca gcttcgtgtg cggcgccacg gcaaagggcg cggacattcc cggcttccat
     1441 tcgccgcgcg tctccagttc gcgcgtgatc tcgcggaccg ttcccccttc ccacatggcg
     1501 tacatccgcc cgccatgacc gacgacatga atgaaggcgc cgttcttgag cggcccggga
     1561 tcgccgccgg gaccgacgag gctgggtgcg accggaaagg gtttggcgac gcccccgtag
     1621 atggcgcgcc cggctcgccg ttcggcggcg agtccgggag tcgcgacgta gcggttgcga
     1681 tagcgcgcgc ggccgctttc cagatacacc gcgtgcagca tgccgtcgcc gtcgaagggc
     1741 caggtgtagc tgatcggtgc aaaggccgga ttgggaccgt tgcgcacata cacgcccgca
     1801 agctccgccg gcaacttccc gctgacggga agatcgctcg cgtcgcattc gtcatgaacg
     1861 ggagcgaaac cgtcgcgcag atacggatcc gtgctctccc acgaagcttc cgtgtcccgc
     1921 agcccgatcg aaacgagttc atccatcgcg tgctccgcgc gacagtgtac caagcagcgc
     1981 gcggccgtgc ttggcagggt gtcgaaaaac accctgtcaa ggcgggacag ggatgtcccg
     2041 cccgcgaatc aagcgcgcaa gcgcttggtt cgtcgttagc gaatccggac atgtgccgga
     2101 ttcgcgactt gaaaaagggc aggacagccc ttgttcaaca aactgttacg tatttccgtt
     2161 cgcggagcgc cgcgggcctc catcgccccc gacgagaggg aaacgccccg gcgcgacgag
     2221 cacgcaggcg agcgtaagaa tcgagagcag cgcgatgccc cagtagatat gatgtgtcgc
     2281 gacgtagatc gcgtggcgga ggaagccgcg cgccgccgcg ccgagcgccg gatcgtgaag
     2341 cgcgtggatg acctcgttta cctgctgcgg caagcggctg cggagcgcgg ccggcgcggc
     2401 cgcaagctgc gatgcgagcg cggcattgaa gatcgcaccg aaaattgcgg cgccgagact
     2461 ttgcccgaga tagcgcgcaa acatgttggt gccggtgacg acgccgcgct cctcccagcc
     2521 gaccaccgat tgcacgccga cgagaagcgg cgtcgaaagc aggccgaaac ccgcaccgag
     2581 cacgacctga tcgagcacca ccgcccacac cggcccggga tcgggcagcg cgagaaatcc
     2641 gacggccgcg agaaccatca gcacaccgcc tccgagcgca gagtcgcgaa agcccaagcg
     2701 cagatagatg cgcccggaaa gcgccgatgc gatcggccag ccgacactca tggccgcgag
     2761 caggaagccg gccgcgatcg cgcccagtcc atagaccgat tgcgcgaagg tgggaagata
     2821 ggcaatcggc cccatcatca cgatccccat gccgaccatc gcgagattgg atccggcaag
     2881 tacgcgccgg cgccacaacc aggccggcag gatcggctcg gcggcgcggc gctcgaccgc
     2941 gatggtgagc gccgcgaaca cgaggacgaa cgcgaacacg atcccgctcg gaagcgacag
     3001 ccacggccat tgctcgccac cctgaagcag gcccaggacc agcatgccga cggtcgcaag
     3061 caccagcgcc gcgccggaaa aatccacgcg atggcggctg cgcgcaaaac tttcgtgaag
     3121 gttgcgcccg atgagaacca gcgagaccgc gcccaccggc agattcacga gaaaaatcca
     3181 gcgccacgaa gcgtattcgg cgaacgcacc gccgagcgcc gggccgatga tcgcggagag
     3241 tccccacatg ctcgaaagcc agccctgcac tcgcgcgcgc tcgcgaatcg agaagagatc
     3301 gcccgcgagg gtgttcacgg tcgccatgat cgagcccgcg ccgagtccct gaaacgcgcg
     3361 aaacacgatg agcgagagca tgctccaggc acccgcgctg gccgccgagc cggcaagaaa
     3421 gaccagcgtg cccacgatca gaacgggctt gcgcccgaac tggtcggcga gcttgccgta
     3481 cacgggaatg gtcacggtct gcgcgagcac gtagatcgag aacacccagg cgaaaagcga
     3541 gaagtcgccg agatcgccga cgatctgcgg aatcgcggtg gagacgatgg tggaatccat
     3601 ggccgcgagc atcatcgtcg ccatgagtcc ggcgagaatc cagcgccgcg agccggcgct
     3661 ttccgcggtg ccgctcattt tcccgcggga gtgtgggctg cttcttgcgc gccttcgggc
     3721 aggaagattt cgtaatcgtt cggtccgttc atgtagccgg caatcttggc gctcttcatg
     3781 caagggtgcg tcatcgccag ctccaccgcg cgtttgttca gcaggccgac gccgtcgttg
     3841 gcgagaaaat cgtcgagcgg catccaccgg gccctggcaa gctcggtctc gtccatgacg
     3901 atggcgcggc tgagcggcgt caggcggcag atgacgtaga gatcggacgt cgcgaactgg
     3961 ccgtgatgat gatggcgcag cgcgacgatc ccctcgaagc gcgcttcgat gccggtttcc
     4021 tcgcgcactt cgcgcacgac gccgcgggca atgtcttcgc cgggttcgag cgtgccgccg
     4081 ggaagtttcc agtactccgg acgggattcg agttcgtagc gttcgagcac cgtcaggagt
     4141 tcacgcgctt cggagatcac gacggcgccc acgcccagcg tatgggtcgc gcagggcggc
     4201 acggatgccg actcttccag cgggcacacg agcatcaatt gttcggagga gcaatcgtga
     4261 aattcgaatc ccgccgcgag cgcggctgcg atcaatggcg cgcgttcgcg ggcaagactc
     4321 agccagacca tcgtcgcccc ctgcgccttc cactgttcga gcgaatgcgc gagcaggctt
     4381 gcgaaatgcg ccgcatcccc gggcagagcg ccggaatcca cttcgacgcc gccgtatttg
     4441 ttgcggcgtg caggcaggat gttcatctca ggaagcgttg gcaacggacg tcaacttcaa
     4501 ttgcgcggca ttcgatgcgc gcggcgcgat ccgcttgcga aacgccgcga tcgcatcgcg
     4561 cccggccgcg accagcaggc cgtcgccgac cagcagccgg gtcgatcccc gcggcaggac
     4621 ttcgcgctcg ccgcgaatga ggccgacgat cacgacgccc atcctgtcga ggccgagcga
     4681 acgtatcgtg cgacccgcga gcggcgcgcc cgcggcgagg ctcaggtgat acaaacgctc
     4741 gccgcggccg tcgagcgtga cgccctcgcc gcttgcgagc gccgtttgca tttcctcgtc
     4801 gagcaactct tcgccgagct tcagttcatg gcggcgcagg agattctcca tcgtgtgccg
     4861 atacacctcg cgatacacgg ggctgtcggc cggccccggc acctgcgcct cgtaaagcgt
     4921 cggatagcgc gcgcggcgcg tgagcgccat ctgcaccacg aaggcgagcg cgacggcgat
     4981 catggtcggc aggacgagct ggtagccgcc ggtcatctcg atcaccatca ccagcgcggc
     5041 gatcgggatg cgcgcggcgc cggcgaaaag cgccgccatg ccgacgacgg cgagcgcgga
     5101 ggcgctgatc tcaagatgaa acaggccgag cagccccgcg aaggtcgcac cgagcatcgc
     5161 gcccacgtag agagccggcg cgaacacgcc gcccgatccg cccgagccga cggtgagcgc
     5221 gagcgcaagg atcttgccga gcgcgagaat cagcagcaac cagatcgcaa gcccggcgcc
     5281 accctgcaag gcgaactgga tatagccgta gccgccgccg aggagcggcg gcagcgcgat
     5341 gccgatcagt ccgaccgcga ggccgccgat cgcgggcttg aaatgatcgg gaatcgcgag
     5401 cgcgcgaaaa ccgtcgcgaa cgcgatagaa cacggtcggc aacagcgcgc cgaacacgcc
     5461 cgcgacgaga ccgagaatcg cgaaccagac gaggttcacc gccgcgccca tgccgacatc
     5521 ggaagacaac acgaacagcg gcgtgaatcc gtcgaacgag ccgatgatgg cataggcgac
     5581 ggccgcggaa atcagcgtgt agatgagcgc gccgccctcg aaggccatgc ccgagtagag
     5641 cacttcgacc gcgaagattg cggtgccgag cggacttttg aagatcgcgg acaagcccgc
     5701 cgccatgccc atgaggatga tgatgcggcg ttcgtcgtcg gggagcttga gccagctaga
     5761 tgcgatcgcg cccacccccg ccgcgatctg cgccgtcggc ccctcgcgtc ccgccgagcc
     5821 gcccgagccg atcgtgatcg cgctcgcgat gcccttgatg agcggaatgc gcgcacgcat
     5881 ccgcccgcgg ttcacgtgaa aggcgcgaac cgccgaatcg gtaccgtggc cttcggcctc
     5941 cgggcagaac gtatagatca gaaagccgac gacgaggccg cccaccgtgg tcgcgagcgg
     6001 cacccaccag taccaatgcg tgaagggttc gggcgccctg cccgcggcat gcgccacggc
     6061 cacgtttatc gtggagtaat gaccgagaaa cccgaggatg tacatgttcg cccagtgcag
     6121 cgcgagcagg aacagctccg cgcccagccc gccgacgagc ccgaggaaca cgctcatgat
     6181 gagcaaccgc ggcgtgcgat cgagcttcat caatcccatc agaaattccc ggtcgcggac
     6241 gcatccgaac gagcgcttat catataggcg atccgcaggt cgccgaaaaa cgcagcgagc
     6301 gaaggcggtt tgggcgccgc cggagcgcag gaaccgaagt gtacacgcga gtacatgagg
     6361 atttcgagca ccgccggcgc ccaaaatgcc gagcgcagca gtttttcggt gacctgcccg
     6421 ggcctgtagc tcagttggtt agagcaggag actcataatc tcttggtcca aggttcgagt
     6481 ccttgcaggc ccaccaacga cggacccgca atttccgtgc aagccggaga ttactgggca
     6541 accggtcaag aaaggccctg cggcagcaat gcggcagggc cttgccgtac tcggaacgtc
     6601 gattgcaggc cgcgatgagc atccggcatc gtgcggcggg cgaggcggcg ccggcgctgt
     6661 tggtcgcgat cgtctgactg gtgtgccgct cgccctgctg cacgctctgc gggtcgtcgc
     6721 ggtcatcaag gccaaggatt tcgacgatgc gctggatatc gccaacgcca ccgacttcgg
     6781 gctcaccggc ggcctcatca gcaacaatcg cgcgcatctg gagaaggcgc ggcgcgagtt
     6841 catggtcggc aa
//
LOCUS       DASUJG010000232         6918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1414118, whole genome shotgun sequence.
ACCESSION   DASUJG010000232 DASUJG010000000
VERSION     DASUJG010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6918
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414118"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VFX38_04940"
     CDS             complement(<1..635)
                     /locus_tag="VFX38_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005221733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5314239.1"
                     /translation="MNRGNLFAAARRVLAGGVDSPVRAFGAVGGDPIFVERAEGAYLY
                     GADRRRYLDYVGSWGPMIAGHAHPRVIAAVQKTAASGTSFGAPSELETELAEAVIARV
                     PSIERLRFVSSGTEATMSALRLARAATGRELIVKFEGCYHGHSDSLLVAAGSGALTFG
                     VPSSPGVPAALAHLTLVLPYNDPAAVVGLFAERGGDIAAVIVEPVAGNMGCV"
     gene            740..1159
                     /locus_tag="VFX38_04945"
     CDS             740..1159
                     /locus_tag="VFX38_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314240.1"
                     /translation="MKILVLAIALAALCAGCANVRTAQSDEAASDPNFPLAADRLPWK
                     PAFTPAGSAAVCQSWMLGEWQYTVLYVHDGGSRGWVLQGYDAQRLGLPGGTVIKDSAA
                     AAAAGIALICSARSRDASASPPDRSHRQAGREAQRNR"
     gene            complement(1196..1801)
                     /gene="thiE"
                     /locus_tag="VFX38_04950"
     CDS             complement(1196..1801)
                     /gene="thiE"
                     /locus_tag="VFX38_04950"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX5314241.1"
                     /translation="MLTDERLGNRLETVIASALAGGARLIQYRDKTADLARRERETKA
                     LRALTRRHHALLIVNDDPDLAAEVEADGVHLGCDDPDPLAARARLGDTAVIGVSCYDS
                     LALAHEAVAAGADYVAFGSFFSSPTKPAAVRAPLALLTAAKRELAVPLCAIGGITPEK
                     TAALVAAGADLLAVVSSVLFAADVAAVARRMATAFEQDAAT"
     gene            complement(1858..2613)
                     /locus_tag="VFX38_04955"
     CDS             complement(1858..2613)
                     /locus_tag="VFX38_04955"
                     /inference="COORDINATES: protein motif:HMM:NF020132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEX5314242.1"
                     /translation="MKRPTLLVIAGLDPSGGAGLAADIEAAIALGAYPAPVASLLTVQ
                     DSRRVLQVMPLDPALVRAQAEAVLADLRVAAVKLGALGTAAIGTAVADLLERHRQIPV
                     VTDPVLAASGGGEVLGEPALLALYRERIFPRSLVATPNRAELAALAPGDGAAELVALG
                     LPACLVTDGDGRSERIVHELHTVQGIRLLPGGPRLPGVFHGTGCSFASAIAARVAVGD
                     SLEEAIDAAERFLRRALAQAFVPGAGQAVPGRW"
     gene            2688..2840
                     /locus_tag="VFX38_04960"
     CDS             2688..2840
                     /locus_tag="VFX38_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEX5314243.1"
                     /translation="MCVVCGYIYDEAEGDPVGGVPAGTRWDDLPITWCCPDCGASKDD
                     FEMVEF"
     gene            2899..3729
                     /locus_tag="VFX38_04965"
     CDS             2899..3729
                     /locus_tag="VFX38_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014994592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX5314244.1"
                     /translation="MPIHTRPRAARAARGALGIVAACVLLAGCVTTPTGRSELVLVPA
                     DQMARMGDQAFAQARKETPVSHDAAATRFVQCVAKNITALAPMSYSWQVTLFQSKEAN
                     AFALPGGHIGVYTGLLKYARTQDELAAVIGHEVGHVLARHADARVSTQLLAGIGLQAA
                     DAAIGGSGPTHQAIMSALGIGTQVGVMLPYSRSQESEADLIGLNLMSEAGFDPEASIQ
                     LWHNMQSIGGEPPAFLSDHPSTSDRIANLQAHMPQALSQYRQAKAKGRNPQCGSAPRS
                     "
     gene            3771..3908
                     /locus_tag="VFX38_04970"
     CDS             3771..3908
                     /locus_tag="VFX38_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314245.1"
                     /translation="MLAIFHPFESTISDPLIDGLAIAIVLAVNAALAAGADLRRGIHT
                     M"
     gene            4062..4508
                     /locus_tag="VFX38_04975"
     CDS             4062..4508
                     /locus_tag="VFX38_04975"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX5314246.1"
                     /translation="MALVYLPMQSGVYEVLHLQWNWFGKFLAVVWAVLFLAFGPLSFQ
                     DAGVRKPRSGTGKSAAIVVVVLAAVAFSAHWFLSSGAPRSVETLLYQLTMPAIAEELV
                     FRGVLFTVLERAFREAMHGAHWWQSRAVWLTAIAFALFARVGHIAR"
     gene            complement(4709..5851)
                     /locus_tag="VFX38_04980"
     CDS             complement(4709..5851)
                     /locus_tag="VFX38_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HEX5314247.1"
                     /translation="MRSGDFERVADRRGTASVKWDRYGGRDVLPLWVADMDFAAPPAV
                     LDALSRRVAHGIFGYTHASAALTEAVLAHVARRHGWKIEAEWLVWLPGAVAAIHAACR
                     LVEAGAPVVSCPPIYPPFLAAPANMGRRRLEVPLAADEDGRAVLDLDGLARAFAQGPG
                     LFLFCNPHNPTGRVFDATELAALAERLLAQDMLVVSDELHADLVLEPGLRHRPLAAVS
                     PALAERTITLFAPSKTFNLAGLGIGYAVIPGTELRRRFRAAIDGILPYVNALAYAAAE
                     AAYTECGDWLAALLAYLRDNRDHVTGVLAGIAGIAARPPEGTYLYWLDLRGTGIDNPV
                     AHLERHGVGLSDGADFGAPGFARLNFGCPRATLEAACSRLTDAFVP"
     gene            complement(5863..6072)
                     /locus_tag="VFX38_04985"
     CDS             complement(5863..6072)
                     /locus_tag="VFX38_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015257231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314248.1"
                     /translation="MEKKYGVRITMPGDDPLRAEHLLGADWEAKRWFDTAEERDAWLE
                     EQGYEHVYSRRGDIQSQVYEKIERE"
     gene            complement(6103..>6918)
                     /locus_tag="VFX38_04990"
     CDS             complement(6103..>6918)
                     /locus_tag="VFX38_04990"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5314249.1"
                     /translation="AVLLWMYPWYASLKRDPFVSLGLFRDRNYSLACLMMLLLGMVLL
                     GTIALLPPLMQELLDYPVFTTGLILAPRGVGAMIMMILVGRLIRRVDPRYVIVSGLLT
                     NAFALFELSRFTLQADPFFIVWTGFIQGVALGQIFVPLTTVAFSTLPARLRNEATPVY
                     NLLRNIGSSIGISIVFTLLGHNTQLNHAALASQFTVYGDRLLRFLHAMPLPRDAALAL
                     LNNEITRQSALIAYNDDFLLMAALMVLIVPLPFFMRYRFGASVPEEEGALAME"
BASE COUNT         1222 a         2264 c         2316 g         1116 t
ORIGIN      
        1 acgcaaccca tgttgccggc gaccggctcg acgattacgg ccgcaatatc tccgccgcgt
       61 tcggcgaaca agccgacgac ggcggccgga tcgttgtacg gcagcacgag ggtgagatgc
      121 gcgagcgcgg cgggcacacc cggcgaagac ggaacaccga aggtgagcgc gccggagccg
      181 gcggcgacga gaagagaatc cgaatgtccg tgatagcaac cttcgaactt gacgatcagt
      241 tcgcgcccgg tcgcggcgcg ggcgagacga agcgcgctca tcgtcgcctc ggtgcccgag
      301 ctgacgaagc gcagccgctc gatcgaagga acgcgcgcaa tcaccgcctc ggccagttcc
      361 gtttcgagtt cgctcggcgc gccgaacgaa gttccgctcg cagccgtttt ctgcacggct
      421 gcgatcaccc gcggatgcgc atgccccgcg atcatcggcc cccaggagcc gacatagtcg
      481 aggtagcgcc tccggtcggc gccgtagaga tacgcgcctt cggcacgctc gacgaagatc
      541 ggatcgccgc ccacggcgcc gaaggcgcgc accggcgagt cgacgccgcc ggccagcacc
      601 cgtcgcgcgg ccgcgaaaag atttccacga ttcatgtgag ccacctcgcc atgcgcatcc
      661 atcgtagcgc ggcttcgtat caccgatcgt tgcgctgccg cggcgtatag gaaaaaacca
      721 ttgcatcgcg gcaggactca tgaaaattct cgttctcgca attgcgctgg ccgcgctctg
      781 cgcaggctgt gcgaacgtcc gcacggcgca aagcgacgaa gcggcatcgg acccgaactt
      841 cccgcttgcc gccgatcgcc tgccctggaa gcctgcgttc acgcctgcgg gttcggcggc
      901 ggtctgtcag tcctggatgc tgggcgagtg gcaatacacg gtcctctacg tccacgatgg
      961 cggctcgcgc ggctgggtgt tgcaaggcta tgacgcgcag cgcctgggcc tgccaggcgg
     1021 caccgtgatc aaggattccg ccgctgccgc cgcggccggc atcgcactca tctgttcggc
     1081 gcgctcgcgg gatgcaagcg cttcgccacc ggatcgaagc cacaggcaag caggtaggga
     1141 agcccaaagg aatcggtgaa gcgcaaacac catccggcga gcgcagttct gctctctagg
     1201 tcgcagcgtc ctgctcgaaa gccgttgcca tgcggcgggc aacggccgcg acatcggcgg
     1261 cgaaaagcac ggaggaaacg actgcgagga gatcggcgcc cgcggcgacg agcgcggccg
     1321 tcttttcggg cgtgatgccg ccgatcgcgc acagcggaac ggcgagttca cgcttcgcgg
     1381 ccgtgagcaa tgcgagcggc gcgcgcaccg ccgcgggttt ggtcggcgag gagaaaaaac
     1441 ttccgaaggc aacgtagtcg gcgccggcgg cgaccgcctc atgcgcgagc gcgagcgagt
     1501 cgtagcagga cacgccgatc actgcagtgt cgccgagacg tgcgcgcgcc gcaagcggat
     1561 cgggatcgtc gcatccgaga tgcacgccgt ccgcttcgac ttctgccgcc aagtcgggat
     1621 cgtcgttgac gatcagcagg gcgtggtgtc gtcgcgtcag cgcacgcagc gctttcgttt
     1681 cgcgttcgcg cctggcaagg tccgcggttt tgtcgcggta ttgaatcagc cgcgcgccgc
     1741 cggcaagcgc ggaagcgatc accgtttcca ggcgattgcc gaggcgctca tcggtcaaca
     1801 cgtacaaacc gcggagccga gcgggaacta cgcgctccgt cccggttccc gaagccatca
     1861 ccagcgcccc ggcacggcct gcccggctcc ggggacgaac gcctgggcga gcgcccgacg
     1921 gagaaatcgc tcggcggcat cgatcgcctc ttcgaggctg tcgccgacgg cgacgcgcgc
     1981 cgcgatcgcc gaggcgaagc tgcaacccgt gccgtggaag acgccgggca ggcgaggccc
     2041 tcccgggaga agacggatgc cctggaccgt gtgcaattca tgcacgatcc gttcgctccg
     2101 cccgtcgccg tcggtgacga gacaggcggg aagcccgagc gcgacgagtt cggcggcacc
     2161 gtccccgggc gcgagcgccg cgagctccgc acggttgggc gtcgcgacga ggctccgcgg
     2221 gaaaatccgt tcccggtaca gtgcgagcag cgcgggctcg ccgagcacct caccgccgcc
     2281 gcttgccgcg agcaccggat cggtcaccac cggaatctgg cgatgacgtt ccagaagatc
     2341 cgcgaccgca gtcccgatcg cggccgtccc cagcgctccg agcttcaccg cagccacccg
     2401 cagatccgcg agcacggcct cggcctgcgc gcggacgagc gccggatcga gcggcatcac
     2461 ctgcaaaacg cggcggctgt cctgcaccgt gagcaggctc gcgaccggcg ccgggtacgc
     2521 gccgagcgcg atggcggctt cgatgtcggc ggcgagcccc gcgcctcccg aggggtcgag
     2581 accggcgatg acgaggagcg tcgggcgttt caccggcctg cggcgctcat gagaaaatat
     2641 ggcggcatcg gctcgaatcc ggctccggcg tggaatatcg caaatacatg tgtgtagtct
     2701 gcggctacat ctatgatgag gccgaaggcg atcccgtggg cggagttccc gcgggtacgc
     2761 gctgggacga tctgccgatc acctggtgct gccccgactg cggcgcgagc aaggacgact
     2821 tcgagatggt ggaattttga tcgcgaacgc gtttccaatc ggagtattct gaagcttcgt
     2881 ctccgggaca ggcccaccat gcccattcat acccggcctc gcgccgcccg agccgcccgc
     2941 ggggctctcg gcatcgtcgc cgcctgcgtc ctccttgccg gctgcgtcac cacgccgacc
     3001 ggacgcagcg aactggtgct cgttccggcc gaccagatgg cgcggatggg cgatcaggcc
     3061 ttcgcccagg cccgcaagga aaccccggta tcgcacgacg ccgccgccac ccgcttcgtc
     3121 caatgcgtcg cgaagaacat caccgcgctg gcgccgatgt cgtattcctg gcaggtcacg
     3181 ctctttcaga gcaaggaagc gaacgccttc gcgcttccgg gcgggcacat cggcgtgtac
     3241 acggggctcc tcaagtacgc gcgcacgcag gacgaactcg cggcagtgat cggccacgaa
     3301 gtcggccatg tccttgcccg tcatgccgat gcccgggtgt ccacgcaact gctcgccgga
     3361 atcgggctgc aggcggcgga tgcggcgatc ggcggaagcg gcccgaccca tcaggcgatc
     3421 atgagcgcgc tcggcatcgg cacccaggtc ggcgtgatgc ttccctacag ccgatcgcag
     3481 gaaagcgagg cggatctcat cggcctgaac ctcatgtcgg aggccggctt cgaccccgaa
     3541 gcctccatcc agctctggca caacatgcag agtatcggcg gcgagccgcc cgccttcctt
     3601 tccgaccatc cctcgacctc ggatcgcatc gccaacctgc aggcgcacat gccgcaggcg
     3661 ctgtcgcagt atcggcaggc gaaggccaag ggcagaaatc cgcaatgcgg ttcggcgcct
     3721 cgcagctagc agagtatcca gctactccgg agtgcgtcgg cctcggcccc ttgctggcca
     3781 ttttccatcc gtttgaatcg acgatcagcg acccgctcat cgacggcctg gcgatcgcga
     3841 tcgtgctggc ggtcaacgcg gcgttggccg ctggtgcaga tttacggcga ggcatccata
     3901 cgatgtagca acaggagggg ttggcgttgt cggcgtactt gcttggcatt cccgtcgcaa
     3961 tttgcctagt tccacctatc gccgtctcgc tggtgggcac gtctcgcata cgctctgctt
     4021 tgtgctggtc tgccctgttc cttgggcttc tcatcctgga catggcgctt gtctacctcc
     4081 cgatgcaaag cggcgtgtac gaggtgcttc acctccaatg gaattggttc ggcaagtttc
     4141 tcgcagttgt ctgggctgtg ctcttccttg cgttcggccc gctctcgttc caagacgcag
     4201 gtgttcgcaa gccgcgatca ggtactggca agtcagccgc catcgtcgtt gtcgtactgg
     4261 cggcggtcgc cttcagcgca cattggttcc tgtcgagtgg cgcgccgcgg tcggtggaga
     4321 ccttgctgta ccagctcact atgccggcaa ttgccgagga gctcgtgttc cgcggcgtac
     4381 ttttcacagt acttgagcgc gcattccgcg aggcaatgca cggtgcacat tggtggcaaa
     4441 gtcgtgctgt ttggctaacg gcaattgcat ttgcgctttt tgcacgggtg ggccacatcg
     4501 cgcggtagtt tccacttcaa tgcaacggcg tgcatcttcc cgtttgtctt tggcatcatt
     4561 gccggcgcgc ttcgcaagcg cacgcgctcg ctgtggtttc caatggcgtt gcatagcgcc
     4621 gtggattttg ctgcggccgt tttcccatga cggcgcggat gctgctgact actcggtcaa
     4681 ccggccgctg tcacggcatg gatgggaatc acggcacaaa agcatctgtc aaacgggagc
     4741 acgccgcctc cagcgtcgcc cggggacagc cgaagttgag ccgcgcgaag ccgggcgcgc
     4801 cgaagtccgc accatccgaa agcccgacgc cgtgccgttc cagatgcgcc actggattgt
     4861 cgattccggt cccgcgcaga tcgagccaat agagatacgt gccttcgggc ggccgcgccg
     4921 caatgccggc gatgccggcg agcactccgg tcacgtgatc gcggttgtcg cgaagatacg
     4981 cgagcaatgc tgcgagccag tcgccgcact cggtgtaggc tgcttcggcc gcggcgtagg
     5041 cgagcgcatt gacgtagggg agaattccat cgatggcggc gcggaagcgg cggcgcagtt
     5101 cggtgccggg aatcaccgcg tagccgatcc cgagaccggc gagattgaag gttttcgaag
     5161 gggcgaagag cgtgatcgtg cgctccgcca gcgccggcga aacggcggcc agcggccggt
     5221 ggcgcaggcc cggttcgagc actagatcgg cgtgcagctc gtcggagacg acgagcatgt
     5281 cctgcgcgag cagacgctcg gcgagcgccg ccaactcggt cgcatcgaac acccgtcccg
     5341 tcgggttgtg gggattgcag aaaagaaaga gccccggccc ctgcgcgaag gcgcgggcga
     5401 gcccgtcgag gtcgaggacg gcgcggccgt cctcgtcggc tgcgagcgga acctccagcc
     5461 ggcggcggcc catgttcgcg ggcgcggcaa gaaagggcgg atagatcggc gggcaactca
     5521 cgaccggcgc gccggcttcg acgaggcggc aggccgcgtg gatcgcggcg acggcgccgg
     5581 gaagccacac gagccactcc gcttcgatct tccagccatg gcggcgggcg acatgcgcga
     5641 gcactgcctc ggtaagcgcc gcggaagcat gcgtgtagcc gaagatgccg tgggcgacgc
     5701 gccgcgagag cgcatcgagc accgctggcg gcgcggcaaa gtccatgtcc gcgacccaca
     5761 gcgggagcac gtcgcggccg ccgtagcgat cccatttcac gctcgccgta ccgcgtcgat
     5821 cggcaacgcg ctcgaagtcg ccgctacgca tcggccggag cgctattcgc gctcgatctt
     5881 ctcgtagacc tgactctgga tgtcgccgcg gcgcgaatag acatgttcgt agccctgctc
     5941 ttccagccag gcatcgcgtt cctcggccgt gtcgaaccag cgcttcgctt cccagtccgc
     6001 gccgaggaga tgttccgcgc gcagggggtc gtctccgggc atggtgatgc gaacgccgta
     6061 tttcttttcc acgaatgaac cgccgcttcg atgagggaaa ttttactcca tcgcgagcgc
     6121 gccttcttcc tccggcacgg aggccccgaa gcggtagcgc atgaagaacg gcagcggcac
     6181 gatgagcacc atcagggccg ccatcaggag aaaatcgtcg ttgtaggcga tgagcgccga
     6241 ctgccgggtg atctcattgt tgagaagcgc gagcgccgca tcgcgcggca gcggcatggc
     6301 atgaagaaaa cgcaggagcc gatcgccgta cacggtgaac tgcgaggcga gcgccgcatg
     6361 gttgagctgg gtgttgtggc cgagaagcgt gaagacgatc gaaatcccga tactcgagcc
     6421 gatgttgcga agcaggttgt acacgggagt cgcctcgttg cgcagccgcg cgggaagcgt
     6481 cgagaaggcg accgtcgtga gcggcacgaa aatctgcccg agcgcgacgc cctggatgaa
     6541 gcccgtccag acgatgaaga agggatccgc ctgcagcgtg aagcgactga gttcgaagag
     6601 agcgaaggcg ttggtgagaa gccccgagac gatgacgtaa cgcggatcga cgcgccggat
     6661 gagccgcccg acgagaatca tcatgatcat cgcgccgacg ccgcgcggcg cgaggatgag
     6721 cccggtcgtg aacaccggat agtcgagaag ttcctgcatc agcggcggca ggagtgcgat
     6781 ggttccgaga agcaccatgc ccaacagcag catcatgagg caggcgagcg agtagttgcg
     6841 gtcgcgaaaa aggccgaggc tcacgaaggg atcgcgcttg agacttgcat accagggata
     6901 catccacagc agcaccgc
//
LOCUS       DASUJG010000233         2295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_926597, whole genome shotgun sequence.
ACCESSION   DASUJG010000233 DASUJG010000000
VERSION     DASUJG010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926597"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VFX38_04995"
     CDS             complement(<1..274)
                     /locus_tag="VFX38_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314250.1"
                     /translation="MRKALVNLLAIQIFVILAVAAGFYVYDREPAAVAAALYGGAVAV
                     GASLLLAWRMSRAGRARGALNLGGLVLGLLERIVFIVAALIVGFAVL"
     gene            complement(492..1073)
                     /locus_tag="VFX38_05000"
     CDS             complement(492..1073)
                     /locus_tag="VFX38_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314251.1"
                     /translation="MRVPQLMAIAGLMAAALGLAACTSSVGGYKGYQQTPTTNAPKGP
                     YPLAQQTLSNMVATDPSLKRFIDNSYGYAVFPTVSSGAFIVGGSYGEGAAFHSSQMVA
                     RCSISKGSIGAQVGGQAYSEVIFFQSKPYFDNFVAGNFTFSAEISAVALTAGAAANVA
                     YNDGVAVFVLPKGGLMASAAVGGQKFSCHPASK"
     gene            complement(1134..>2295)
                     /locus_tag="VFX38_05005"
     CDS             complement(1134..>2295)
                     /locus_tag="VFX38_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314252.1"
                     /translation="RDADIPILGVFLGRRRGLRITGVLPHFVAREDLFDPDFLWRYWR
                     SPWLVLPRLLSPAIPLARILELFQAHPIHRIPEQSLAVVLGDLRRALGDRALGSVLNR
                     WWAPRFRAAGADLAAPVSRLLAAESRYADLLGGGWGHRRLDLATFREFKPGERERIAA
                     HLAVFARALAAGEAVMIAPEGANSPDGFFQRPRAGARWIAKQGGDELVVLPIGLAYDP
                     AAGGRSRVFVHAGPAFRAGDHAGRREFDDAAACAILRATTLTGSHLAAHLVLRARAGE
                     AMSAIEAGERIGNFASALAAAGLRVAADAGAELESRLGWFVGAGLLARRDGSFVRTQA
                     GEPAPGRENRAALAAYLRNELASVVSLAPGLAQEQGLPAPSYRPQSAPSGLV"
BASE COUNT          427 a          774 c          799 g          295 t
ORIGIN      
        1 gcaacaccgc aaaaccgacg atgagtgcgg ccacgatgaa gacgatgcgc tccagcaacc
       61 cgagaaccag tcctcccagg ttcaaggccc cccgtgcccg ccccgcgcgg ctcatccgcc
      121 aggcgaggag aagcgacgcg cccaccgcca ccgcaccgcc gtagagcgcc gccgccactg
      181 cggcgggctc gcgatcatac acataaaaac ctgccgcgac cgccagaatc acgaagatct
      241 gaatcgcgag caggttgaca agagctttcc gcatccgcgt cggggcggcg cggccttcgc
      301 gcctgtgctt cccggacggc gagcatttta cccggtcacg cgcatccgtt caacgtgaag
      361 gcgtcgaaat accgcagcgc gcgatatcca ggcgatggag cgccacaaac tcgcgccgtt
      421 ccgcgcgaag ggaccggcgc gggctccgca tcccgcggag cccttgcggc tccgcggggt
      481 ggatacgatg cctacttcga ggccgggtgg cagctgaact tctggccgcc gaccgccgcc
      541 gaagccatga gtccgccctt gggaaggacg aacacggcga cgccatcgtt gtaggccacg
      601 ttggcggccg cgccggcggt gagagccacg gccgagattt ccgcggaaaa cgtgaagttg
      661 ccggcgacga agttgtcgaa atagggcttg gactggaaga aaatgacctc gctgtaagcc
      721 tggccgccga cctgggcgcc gatcgaaccc ttggagatgc tgcaacgggc aaccatctgg
      781 ctgctgtgga aggccgcgcc ctcgccgtag gagccgccga cgatgaacgc gcccgaactc
      841 acggtgggaa acacggcata gccgtaggaa ttgtcgatga agcgcttgag gctcggatcg
      901 gtcgcgacca tgttcgaaag cgtctgctgc gcgagcggat acgggccctt cggagcgttg
      961 gtggtcggcg tctgctgata tcccttgtag ccgccgaccg aactggtgca ggccgcgagc
     1021 ccgagggccg ccgccatcaa tccggcaatc gccatcaatt gaggtacacg catgagagtc
     1081 tcctgtttct gcttgtcgaa ggtacgattc aaggctaaca tccgcgccgc cgctcaaaca
     1141 aggcccgagg gagcggactg cgggcggtag gagggcgccg gcagcccctg ctcctgcgcc
     1201 agcccgggag cgaggctcac gacggaggcg agttcgttgc gcaaataggc agcgagcgcc
     1261 gcacgattct cgcggccggg agcgggctcc ccggcctgtg tgcgcacgaa gctgccatcg
     1321 cggcgggcga gaaggcccgc gccgacaaac cagccgaggc gggactcgag ttctgccccg
     1381 gcatcggcag ccacccgcag gcccgcggcg gcgagcgcgg aggcgaaatt tccgatccgc
     1441 tcacccgcct cgattgcgga catggcttcc ccggcgcgcg cgcgcagcac cagatgcgcg
     1501 gcgaggtgcg atccggtcag ggtcgtcgcg cggaggatcg cgcaggccgc ggcatcgtcg
     1561 aattcacgcc gccccgcgtg gtcgccggcg cgaaacgccg ggcccgcatg cacgaacacc
     1621 cgcgagcgtc cgccggcggc tggatcgtag gcaagtccga tcggaagcac caccagctcg
     1681 tccccgccct gttttgcgat ccatcgggcg cccgcgcgcg ggcgctggaa aaagccgtcg
     1741 ggcgaattgg ctccctcggg cgcgatcatc accgcctcgc cggcggcgag cgcgcgggcg
     1801 aagaccgcga gatgtgccgc gatgcgctcg cgctcgccgg gcttgaactc gcggaaggtc
     1861 gcgagatcga gccgccgatg cccccagccc ccgccgagaa ggtcggcgta gcggctctct
     1921 gcggcaagca ggcgcgaaac gggcgcggca agatcggcgc cggccgcacg aaaacgcggc
     1981 gcccaccagc gattgaggac cgaaccgagt gcgcgatcgc cgagagcccg gcgcagatcg
     2041 ccgagcacca ccgcgagact ttgttccggg atgcggtgaa tcggatgcgc ctggaagagt
     2101 tcgaggatgc gcgcgagcgg gatggcaggc gagaggagac gcggcaggac gagccaggga
     2161 gagcgccagt agcgccacag aaagtccgga tcgaagagat cctcgcgggc gacgaaatgc
     2221 ggcagaacgc ccgtgatgcg cagcccgcgg cggcgcccga gaaacacgcc gagtatcggg
     2281 atgtccgcgt cgcgg
//
LOCUS       DASUJG010000234        11733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1537327, whole genome shotgun sequence.
ACCESSION   DASUJG010000234 DASUJG010000000
VERSION     DASUJG010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 11733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11733
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537327"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="VFX38_05010"
     CDS             complement(<1..963)
                     /locus_tag="VFX38_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEX5314253.1"
                     /translation="MALALIAFVFALAVVLGLAARRGKKMDLEQWTLGRRGFGVVFVF
                     LLMAGEIYSTFTFLGASGYAYGRGAPAFYILAYGALAYALGYWLLPPISRYAKEHRLI
                     TQADFFAHRFASRELGIAVSLIGIVAMIPYLALQLEGLGLIVEIASGNAIGARAAILI
                     GTAGLVLYVIASGIRASAWTSVVKDFLLLAVAVFLGVYLPLHAFGGIAPMFHRLAASD
                     PQLFTLHAPQGSLWFLSTVLVSALGFWMWPHAFAYIASARSENVFRKNAMVLPLYQLF
                     MLFIFFVGFAAIGIVPGLKNSDLALLSATIAALPPWVTGIVGGAG"
     gene            complement(948..1184)
                     /locus_tag="VFX38_05015"
     CDS             complement(948..1184)
                     /locus_tag="VFX38_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017248200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3311 domain-containing protein"
                     /protein_id="HEX5314254.1"
                     /translation="MKLSRALLGLLPFVGILAGAYLVNRVEPFVLGMPFLLFWIAAWT
                     VLTAVLMALVYRLDPRNRRDGSARADKEPGWRSR"
     gene            complement(1202..1918)
                     /locus_tag="VFX38_05020"
     CDS             complement(1202..1918)
                     /locus_tag="VFX38_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5314255.1"
                     /translation="MPEGPEITAIVGVGPGLGAALAERFAAGGHALALLSRSARSRGP
                     VAKNIASESRVQGYDCDAGDPASVAVAFARVRDTLGNPTVLIYNAGNYAPGGILEMDP
                     DRFEAAWRANCFGGFLAAREVLPAMLEAGAGTILFTGATAALRGAKGFAGLAVGKFGL
                     RALAQSMAREFAPGGIHVAHVVIDGQIGSPSARERQPDRAEETFLAPGDIAETYWQLY
                     TQPRSTWTQELDLRPDVENF"
     gene            complement(1998..3560)
                     /locus_tag="VFX38_05025"
     CDS             complement(1998..3560)
                     /locus_tag="VFX38_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314256.1"
                     /translation="MATKIRYPGLPIIYVRGFAASMSEIDQTTADPYMGFNLGSTMLR
                     QDAAKDPVRFIFESPLLRLIKDHGYEDAFRHGSDSYDPGTAPAQSIWIYRYYEQSSDT
                     LGTGKLVPIEQFAADLRAFILNVRDAICGDDADERKKFGVYLVAHSMGGLVCRCYLQN
                     ICRHGAPKAEDNTALELVPGKANPHYVDKLYTYATPHNGIDMFGMNTPQLNLAVLGAH
                     QTANFNRVRMREYLKISSPAVGVNDLDGAFAAERCFCLVGSDNRDYEAFFGLSKKGTG
                     PMSDGLVMMKNAWVKGAPRAVTYRSHSGFYGIVNSEAGYQNLRRLLFGDEQVTALLCV
                     DALPLSRAVQAKKDAGAEVRGSYYFDVVARVRAAATFALNEQRYEQGSSVLKTYDELT
                     ADKKPVFLFTGYLLAQARLASDTALMFAIDVGIRVPAFEIDKKFWFDEHFEGFLYRDT
                     ITFAVRAGSIRYGLASANGIGSAPTTATVTATADGGREIRIPLATKAGVLPGFQGHLV
                     LQTQAWNAAPES"
     gene            complement(3575..4168)
                     /locus_tag="VFX38_05030"
     CDS             complement(3575..4168)
                     /locus_tag="VFX38_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX5314257.1"
                     /translation="MSIEDMIRAIQEQLGITADGRAGPETWGAIYARIVPAAKPPPAQ
                     AEDSRSEKNIATLLPEVQPYARALIGKAAAVGITIKVISGTRSYAEQDALYAQGRSAP
                     GPIVTNAKAGYSNHNFGLAFDIGVFEGNKYLAESPKYKAVGALGIEIGLSWGGSWKSF
                     QDQPHFELRPAWAKNLSESQMLAALRQRKTDGEPVFA"
     gene            4488..4563
                     /locus_tag="VFX38_05035"
     tRNA            4488..4563
                     /locus_tag="VFX38_05035"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4521..4523,aa:Ala,seq:cgc)
     gene            4600..4675
                     /locus_tag="VFX38_05040"
     tRNA            4600..4675
                     /locus_tag="VFX38_05040"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4633..4635,aa:Arg,seq:cct)
     gene            5379..5870
                     /locus_tag="VFX38_05045"
     CDS             5379..5870
                     /locus_tag="VFX38_05045"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEX5314258.1"
                     /translation="MIGLAAVAGLGLSRAMAKPPVSGMDKAWLVEAHQINLAEIAVGK
                     MAEQKGQSQAVKNAGRTLVADHQNLDRMLEAGAQKLGVQLPDSPSAAEQEQANSLEQK
                     SGADFDEAFLHMEIQGHQEAIADTRRESKAGSSIAVTNLALKTLPVLESHLRMLEQIG
                     EQQ"
     gene            complement(6040..8214)
                     /locus_tag="VFX38_05050"
     CDS             complement(6040..8214)
                     /locus_tag="VFX38_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX5314259.1"
                     /translation="MEQQPAPDSRGIYRFGPFHLDTAERALYRDGRPVRLRAGALDLL
                     VTLLENAGHLKTREELIASLWPGTVVEEQGLTVSMSALRKALGDEGRVARYIETVRGH
                     GYRFVAPVDTAEPTAADGAGEMPSGEQPASDSHPVARRHRRRVAASALAAGAAILAGA
                     LLWYFLALRPAAPGAAPTVAVLPFENLSGDRANAYFAAGIQDTILSKLAGIGELRVIS
                     RTSTEQYPSHPPHLQQVAHELCATAILEGSVQKSGDRVLINVQLIDVRTQAHVWAEDY
                     TRTLKDVFEVENDVAAQVAGALKATLLPREAAQIRRVATRDPHAYLLLLQANYYADKV
                     SSRNSTKNPAADTRRAIALYRQAIAADPRFALAYARLSLLESYADWYAIVSTPSDATA
                     EQMATRAVAIDPDLPQAHLAMGYVYYYHRRDYAAALAQFERARQDLPNDADVIAAIAY
                     SHRRQGKWQEASADLRKAAALDPRNPHLPYNAGITLAVMGRYAQAQMQFNQALALEPQ
                     NYDAMAQEAWVLLLAGKIASATQALAKVPAGVDPQGLISTIRFKAAALARRPARALDA
                     VDEAPAWVLGPGTVGNVPKSLLQAQAWAMKGDSAQARRLYIKTEDELHQALRDNARDP
                     DLWAALGLVESGLGRHAAAIHDGRRATELLPLSKDALAGPVYLAALAEIYSQAGKNDA
                     ALRLLQRLLAMPAGRTISVEMLRRDPAWDPLRRDPRFQELLQ"
     gene            8499..10958
                     /locus_tag="VFX38_05055"
     CDS             8499..10958
                     /locus_tag="VFX38_05055"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314260.1"
                     /translation="MIAKFGLKALLAAFLLAGNAAVIAFPASAAAAGDRSAAFGAPDA
                     LPAALRPALYGAMARNGDANFRIGKDGCAEAIKSSLRVCFDARGAHFAAAQASLALRL
                     DAWGRGDHLRTLAAVRPEVRPDRVTYAHEAISEWWRLLPIGYEQGFTLAERPAGNGRL
                     TLALAASDPGEMHDGTLDWHGLRYGGLVVTDADGDIVPSKLTSAGDRILITVDDAHAA
                     YPLVVDPLVWLEQKVSSSDGAAGDNFGWSVAISGDNAVVGAKHASVHGNYWQGAVYVF
                     SKSDGVWSETQKLTASDGVADDRLGNSVGISGPVIVVGAPDVTVHGNAFAGAAYVFTR
                     SDDGSWTQSAKLVANDGAAQDQLGASVAVSGSTILAGAELATIDNKAGQGATYVFTGS
                     GDSWNQVQKITADDGGPGDYFGASVAIAGSAAIVGATQEGWNGPGVAYVFENSGGTWN
                     QTGKLTDANGVSGDVFGLGVAISGSTAVVGAPFVTVHGNPEQGAAFVFSDSGGSWTQT
                     QELFDADGGQYRGENFGWDVALSDSTMLIGSYFAQTVFEYTESNGTWTLAQKLSPSDA
                     TADEVFGPTVALSGTDSLVGAQIDTVNGNDGQGAAYFYGAADLGLTLSASGTVGQGQN
                     YVSQTIATNNASAASPAVSATISVPAAASFVAASASQGSCSEDSGVVSCDFGAIAGNA
                     GTATANVTLKATGNVGDTIENTASVAKATPALSARAPTEITGSESCPDGYTSYSGTLV
                     PGANVRIPPYSAAAGEENAILYAPEGFRLYARVDATLWGRYQRGFPATEVHRHGPAGE
                     YRWLVRGNDGGGDYLFCLMHP"
     gene            11170..11252
                     /locus_tag="VFX38_05060"
     tRNA            11170..11252
                     /locus_tag="VFX38_05060"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11204..11206,aa:Tyr,seq:gta)
     gene            11352..11425
                     /locus_tag="VFX38_05065"
     tRNA            11352..11425
                     /locus_tag="VFX38_05065"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11384..11386,aa:Gly,seq:tcc)
     gene            11447..11522
                     /locus_tag="VFX38_05070"
     tRNA            11447..11522
                     /locus_tag="VFX38_05070"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11480..11482,aa:Thr,seq:ggt)
BASE COUNT         2144 a         3609 c         3947 g         2033 t
ORIGIN      
        1 cccggcgccg cccacgatgc cggtgaccca gggcggaagc gccgcgatcg tcgccgagag
       61 gagcgcaagg tcggaattct tcaagccagg cacgatcccg atggccgcga atcccacgaa
      121 gaagatgaag agcatgaaca attgatagag cggcagcacc atcgcgttct tgcgaaacac
      181 gttttcgctg cgcgccgagg cgatgtaagc gaaggcatgc ggccacatcc agaaaccgag
      241 tgccgagacc agcaccgtgg agaggaacca gaggcttccc tgtggcgcgt gcagggtgaa
      301 gagttgcgga tcgctcgccg cgagccgatg aaacatcggc gcgatgccgc cgaaggcgtg
      361 caagggcaga taaacgccga gaaaaacggc gaccgcgagg agcaggaagt ccttgacgac
      421 ggaggtccag gcggaggctc ggattcccga ggcaataacg tacagtacga gccccgcggt
      481 gccgatcagg atcgcggcgc gggcgccgat cgcgttgccg gaggcgatct cgacgatcag
      541 gccgaggccc tccaattgca gcgcgagata cggaatcatc gcgacgattc cgatcaggct
      601 caccgcgatg ccgagttcgc ggctggcgaa gcgatgcgcg aagaaatcgg cctgggtgat
      661 gagccggtgc tccttcgcgt agcgggaaat cggcggcagc agccagtaac cgagtgcgta
      721 cgcaagcgcg ccgtaggcga ggatgtagaa ggccggggct ccgcgcccgt acgcgtagcc
      781 gctcgcgccg agaaatgtga aggtggaata gatttctccc gccatgagca gaaacacgaa
      841 cacgactccg aagccgcgcc gcccgagcgt ccattgttcg aggtccattt tctttccgcg
      901 acgcgcggcg aggccgagca cgacggcgag cgcgaagaca aacgcgatca gcgcgagcgc
      961 catcccggtt ccttgtcggc gcgagcactg ccatcgcggc gattgcgcgg atcgagccgg
     1021 tagacgagcg ccatcagcac tgcggtaagc accgtccagg cggcaatcca gaaaaggaga
     1081 aacggcatgc cgagcacgaa gggctcgacc cggttcacga gataggcgcc ggcgagaatg
     1141 ccgacgaagg gcaggaggcc cagcagcgcc cgcgacagtt tcatcatgag gtggcgtcgg
     1201 ctcagaagtt ttccacgtcg ggacgcagat cgagttcctg ggtccaggtg ctgcgcggct
     1261 gggtgtagag ttgccaataa gtttcggcga tgtcgcccgg cgcaaggaat gtttcttccg
     1321 cgcggtccgg ttgacgttcg cgcgccgaag gcgagccgat ctggccgtcg atgacgacat
     1381 gcgcgacgtg gatgccgccc ggagcgaatt cacgggccat gctttgcgcg agcgcccgca
     1441 gcccgaactt gccgacggca aggcctgcga agcccttggc gccgcgaagc gccgccgtcg
     1501 ctccagtgaa aagaatcgtg ccggcgccgg cctcgagcat cgcgggcaga acttcgcgtg
     1561 cggcgagaaa tccgccgaag cagttcgccc gccatgcggc ctcgaagcga tcgggatcca
     1621 tttcgaggat gccgcccgga gcgtaatttc cggcgttgta gatcagtacc gtcggattgc
     1681 cgagcgtatc gcgcacgcgg gcgaatgcga cggcgacgga ggcaggatcg ccggcgtcgc
     1741 aatcgtagcc ctgcacccgc gattcggaag cgatgttctt cgcgaccggg ccgcgactgc
     1801 gagccgagcg cgagagcagc gcgagcgcgt ggccgccggc ggcgaagcgc tccgcgagcg
     1861 ccgcgccgag tccgggcccg accccgacga tggcggtgat ttccggtcct tcgggcatga
     1921 gttcgcgacc gcgcgggaat ggacgcaagc ttagcacttt gccgcgatcg cggcgaagtg
     1981 gttttcgcgg cgcgcgctca gctttccggc gcggcgttcc aggcctgcgt ctgcaggacg
     2041 agatgtccct gaaaaccggg gagcacgccc gctttcgtcg cgagcggaat gcggatctcg
     2101 cgcccgccgt cggcggtcgc ggttacggtc gcagtcgttg gcgccgatcc gattccgttg
     2161 gccgaggcga gcccgtagcg aatgctcccg gcgcgcacgg caaaggtaat cgtgtcgcgg
     2221 tacaagaagc cctcgaagtg ttcgtcgaac cagaatttct tgtcgatttc gaaggccgga
     2281 acacggatgc cgacgtcgat ggcaaacatc aacgcggtgt cgctcgcgag gcgagcctgt
     2341 gcaagcagat agccggtgaa aaggaaaacc ggctttttgt ctgcggtgag ttcatcgtag
     2401 gtcttgagga cggacgagcc ctgctcgtaa cgctgttcgt tgagcgcaaa ggtggcggcg
     2461 gcgcgcacgc gtgcgacgac gtcgaagtaa taggaaccgc gcacttcggc tccggcatcc
     2521 tttttcgcct gcacggccct ggaaagcggc agcgcgtcca cgcacagcaa cgcggttacc
     2581 tgctcgtcgc cgaagagaag gcggcgcaga ttctggtatc ccgcctcgga attgacgatc
     2641 ccgtagaaac cgctgtggct gcgataggta acggcgcggg gtgcaccctt cacccaggcg
     2701 ttcttcatca tgacgaggcc gtcgctcatc ggtcccgtac cctttttcga gagcccgaaa
     2761 aatgcttcgt agtcgcggtt gtccgagccg acgaggcaga agcagcgttc cgccgcaaag
     2821 gcgccgtcga ggtcgttcac gccgacggcg ggactcgaaa tcttgagata ctcgcgcatg
     2881 cgaacgcgat tgaaattcgc ggtttgatgc gcaccgagca ccgcaaggtt cagttgcggc
     2941 gtgttcatcc cgaacatgtc gatcccgtta tgcggcgtcg cgtaggtgta gagcttgtcc
     3001 acgtaatgcg ggttggcctt gccgggaacg agttcgaggg cggtgttgtc ttccgccttc
     3061 ggagcgccgt gccggcagat gttctgcaga taacagcggc acacgagccc gcccatggaa
     3121 tgcgctacga gatagacgcc gaacttcttg cgttcgtcgg cgtcgtcgcc gcagatggcg
     3181 tcgcgtacgt tcaggatgaa ggcacgcaga tccgccgcga actgctcgat gggaaccagc
     3241 ttcccggtgc cgagcgtgtc ggagctttgt tcgtaatagc ggtaaatcca gatcgattgt
     3301 gcgggcgcgg tccccggatc gtagctgtcg cttccgtgcc ggaaggcgtc ctcgtaaccg
     3361 tgatccttga tcaggcgcaa caggggtgat tcgaaaatga atcgaaccgg atccttggcg
     3421 gcgtcctgac ggagcatcgt cgagccgaga ttgaagccca tgtacggatc ggcggtagtc
     3481 tggtcgattt ccgacatgct cgcggcgaag ccgcgcacgt agatgatcgg caggccggga
     3541 tagcggattt ttgtggccat gatgaactcc tcgctcaggc gaacacgggt tcgccgtcgg
     3601 ttttgcgctg gcgcaaggcg gcaagcatct gcgattcgct caggtttttc gcccaggccg
     3661 ggcgcaattc gaaatgcggc tgatcctgga aggatttcca gcttccgccc cagctgaggc
     3721 cgatttcgat gccgagcgcg ccgaccgcct tgtatttcgg cgactccgcc agatacttgt
     3781 tgccttcgaa cacgccgatg tcgaaggcga gcccgaagtt gtgattcgag tagcccgcct
     3841 tggcgttggt cacgatcggg cccggtgcgc tccggccctg cgcgtagagc gcatcctgtt
     3901 cggcgtagct tcgcgttccg ctgatgacct tgatcgtgat tccgacggcg gcggccttcc
     3961 cgatcagcgc gcgtgcatag ggttgcactt cgggcagaag cgtcgcgatg tttttctcgc
     4021 tgcggctgtc ctcggcctgc gcgggcggcg gcttggcggc ggggacgatc cgggcgtaga
     4081 tcgcgcccca ggtctccggg cccgcccggc cgtccgccgt gatgccgagt tgctcctgaa
     4141 tggcgcgaat catgtcttcg atgctcatcg gcaactccct cgcggcggga ttttcccggc
     4201 gccggtaacg caggcgctcc ctccgattcc ggaagatggg caaggtggtt gctcgttgcg
     4261 cgcacgcggc catcgcgcag acgccgaccg ggaaaggatt gctgacgccg acatacgcct
     4321 tccgccggtg gaaccggcat gtttccggct cctgtctatc acagcgtcga aggtgcgtca
     4381 agaaatgcac ggccgcctcg cgtttcgcgg cgatcggccg cgatggccat ttcgcgtccc
     4441 gccaccgcaa tgctagaatt tccgcaaagc ggccgtccgg tcgcagtggg gcggtagctc
     4501 agctgggaga gcgtcgcgtt cgcaatgcga aggtcgggag ttcgatcctc ctccgctcca
     4561 ccaactcgtt tgccgcccgg cgcagcaggc cgactcatcg ccccggtagc tcagtggata
     4621 gagcgaccgc ctcctaagcg gtaggtcgca ggttcaactc ctgctcgggg caccatctcc
     4681 gctattcgtg cccgggattg ccgcgccgtc gtcgaatcct gcgccacggc gggggacgcg
     4741 cagcgccggt ggcgatcgga ttcccgcgct ggcagaaatc cgccgatgcc actgtggtgt
     4801 agagccgatt tgcctcggct gcgttctatg ggaacgatcg gagcggcccc gacgcgaccg
     4861 ctggggacgc tcggaatcag caagatcgat caggcctgcg ccttgcgccg gcggtgatca
     4921 aacgggcgct ggacggggat agaagcgaaa ggattgatca cccgcacgcc gaagcgctcg
     4981 cgccggtgca gaggatcgcg cgttcgtgcg ccaggacacg gtaacgtccc tgcgcgacgc
     5041 agtaaaaata cggaaatcgg cggcgcagag aaaaccatga ttgtccagtc gtccatgtcg
     5101 ctcgatgatg cccggcgcgt gatcgcagcc agaaaaattc aacggaggga gatcggcagc
     5161 cgacgaatat tgtcgtcggc ggcgtcggcg gcaaccacgt cgccctggcg cagatggacg
     5221 aggcccgctc gagcacgagg atgcagcgat cggggtcggc gcaattggag gagacaagga
     5281 acaggatcaa agggtcgcag aggccgcgca ggcggctctg tgagaaattc gtcaacagac
     5341 atatggagat tgcagtcatg aggaaaacga cgttggtatt gatcggcctg gccgccgtgg
     5401 cagggcttgg cctttcgcgc gcgatggcga agccgccagt ctcgggtatg gacaaggcct
     5461 ggttggtcga agcgcatcag atcaaccttg cggaaatcgc cgtcggcaag atggcggagc
     5521 agaagggcca gtcgcaggcc gtcaagaatg ccgggcgcac cctggtcgcc gatcatcaga
     5581 acctcgatcg gatgctcgag gcgggtgctc agaagctcgg cgtgcaactt ccggactcgc
     5641 ccagtgccgc cgagcaggag caggccaatt cgcttgagca aaaatccggt gcggatttcg
     5701 acgaagcctt tctgcacatg gaaattcagg gtcatcaaga ggccatcgcc gacaccaggc
     5761 gggaaagcaa ggccggttct tcgatcgcgg tcacgaatct cgccttgaag acactacccg
     5821 tactcgaaag ccatctccgc atgctggagc aaatcgggga gcagcaataa aacgagggat
     5881 gagcgcctcg cgtcgaaaga ccgcgcaacc gatcgcaacc ttggtgcctt ttgccgctgt
     5941 gctgcgttgg gtggatccgc tataacaagc cagcgcgctc tccgtgcccg cgagggaagc
     6001 tgccggctgt ctccttgcgg cagcgttctc tcgcgcattt cactgaagca actcctgaaa
     6061 ccggggatcg cgacgtagcg gatcccacgc cggatcgcgg cgcagcatct cgaccgaaat
     6121 ggttcttccc gccggcatcg cgagaagccg ttgcaaaaga cgcagggctg cgtcgttttt
     6181 gcccgcctgc gagtagattt cggccaacgc cgcaaggtac acgggaccgg caagggcgtc
     6241 tttggagagc ggaagcaatt cggtggcgcg gcggccgtcg tgtatcgcgg cggcgtgtcg
     6301 ccccaatccg gattcgacga gaccgagcgc ggcccagaga tccggatccc tggcgttgtc
     6361 acgcaaggct tgatgcagtt cgtcttccgt tttgatgtaa aggcgacgcg cttgcgcaga
     6421 gtcgcctttc atggcccagg cctgcgcctg caacagcgac ttcggaacat tgccgaccgt
     6481 gcccggaccc aagacccagg cgggcgcctc gtccacggca tccaaggccc gggcaggccg
     6541 gcgcgcgagc gccgcggcct tgaagcggat cgtggaaatc aggccctgcg ggtccacccc
     6601 ggccgggacc ttcgcgagcg cctgcgttgc cgatgcgatc ttgccggcga gcagcagcac
     6661 ccaggcctct tgcgccatgg cgtcatagtt ttgcggctcg agcgcgagcg cctgattgaa
     6721 ttgcatctgc gcctgcgcgt agcggcccat cacggcaagc gtaatccccg cgttgtacgg
     6781 caggtgagga ttgcggggat cgagcgccgc cgccttgcgc agatcggcgc ttgcctcctg
     6841 ccatttcccc tgacgccgat gactgtaggc aattgcggcg atgacgtcgg cgtcgttggg
     6901 gagatcctgc cgcgcgcgtt cgaattgcgc gagcgcggcc gcgtaatcgc ggcggtgata
     6961 gtagtacacg tagcccatgg cgagatgcgc ctgcggcaaa tccggatcga tggcgactgc
     7021 ccgagtcgcc atttgctcgg cggttgcatc cgaaggcgtc gacacgatcg cgtaccagtc
     7081 ggcatagctc tcgagcagcg acaagcgggc ataggcgagc gcgaagcgcg gatcggccgc
     7141 gatcgcctgc cggtacaggg cgatggctcg cctcgtgtcg gcggcgggat ttttcgtgct
     7201 gtttcggctc gacactttgt cggcgtagta attcgcctgc aaaagcagaa gatacgcgtg
     7261 ggggtcgcga gtcgcgacgc ggcgaatctg cgcggcttcc cgcggcagga gcgtcgcctt
     7321 gagcgctccc gccacctgtg cggcgacgtc gttctcgact tcgaagacgt ccttcagcgt
     7381 gcgggtatag tcctcggccc agacgtgcgc ctgcgtgcgg acatcgatga gctgtacgtt
     7441 gatcaggacc cgatcgccgg atttctgaac gcttccctcg aggatggcgg tggcgcagag
     7501 ttcgtgggcc acctgctgca agtgtggcgg atggctcggg tattgttcgg tcgaagtccg
     7561 cgagatcacc cgcaactccc ctatgccggc gagcttggaa aggatcgtgt cctggatgcc
     7621 ggccgcaaag tacgcattgg cccgatcgcc gctcaggttt tcgaagggca acacggcgac
     7681 cgtcggcgct gcgcccggag ccgccggtcg aagcgcaagg aagtaccaga gcagcgcgcc
     7741 ggcgaggatt gcggctccgg cggccagcgc gctcgccgcg acgcgcctgc gatgccggcg
     7801 tgcaaccgga tgcgagtcac ttgccggttg ctcgccgctc ggcatttccc ccgcgccgtc
     7861 tgcggccgtc ggttctgctg tgtcgacggg agcgacgaag cggtagccat ggccgcgcac
     7921 cgtttctatg tagcgcgcaa cccggccctc gtcgcccagc gccttgcgca aggcactcat
     7981 gctcacggtg aggccctgct cctcgactac cgtcccgggc cacaacgatg cgatcaattc
     8041 ttcgcgggtt ttcaggtgcc cggcattctc gagcagcgtc acgagcagat cgagtgctcc
     8101 ggccctcagc cgtacgggcc tgccgtcccg gtagagagcg cgctctgccg tgtcgaggtg
     8161 aaacgggccg aagcggtaaa tgccgcgaga gtcgggcgct ggctgttgtt ccattgtgaa
     8221 ccgacgaacg atccggtcaa aatgtctctg gaaaacgcat ccctgcattt ccgccgtgtg
     8281 acgaagccga ttcgcttcga atcaggcaca tgcagcgcca tgcgcgcgcc gctccgatgc
     8341 tccgcccggc gcgaatccgg acggcctgca aggtaaattt aagccggatt tcacgcaaaa
     8401 ttaaggtcac gacggattca cgcggctaat attactaaag tcgctgccgg caacgcaggg
     8461 acgacgtttc cactcgctcg agttacagga gaagcttcat gatcgcgaaa ttcggactca
     8521 aagccctgct ggccgcattc ctgcttgcgg gcaatgccgc ggtcatcgcc tttcccgcga
     8581 gcgccgcggc ggcaggcgac cgatccgctg ccttcggcgc ccccgatgca ttgccggccg
     8641 cactgcgtcc cgccttgtat ggagccatgg cgcgcaatgg cgatgcgaac ttccgaatcg
     8701 ggaaggacgg ctgcgccgag gcgatcaaat cttcgctgcg cgtctgcttc gacgcgcggg
     8761 gcgcacactt cgcggctgcg caggcgtcgc tcgctctgcg cctcgatgcc tggggacgcg
     8821 gcgatcatct ccgaaccctg gccgcagtgc gacccgaagt ccggcccgac cgggtgacct
     8881 atgcccacga ggcgatcagc gagtggtggc gcctgctgcc gatcggttac gaacagggct
     8941 ttacgctcgc cgagcgcccg gccgggaacg ggaggctgac gctcgccctc gccgcgagcg
     9001 atccgggcga gatgcacgac ggcacgctcg actggcacgg cttgcgctac ggcggactcg
     9061 tcgttaccga tgccgacggc gacatcgttc cctcgaagct gacaagcgcc ggcgatcgca
     9121 ttctcatcac ggtcgacgat gcgcacgcgg cgtatccgct cgtcgtggac ccgctggtgt
     9181 ggctggagca gaaggtttcc tccagcgacg gcgcggccgg cgacaacttc ggctggtcgg
     9241 tcgcgatctc gggcgacaac gccgtcgtcg gcgcaaaaca cgcgagcgtg catggcaatt
     9301 actggcaggg cgccgtctac gtgttcagca aatccgacgg cgtatggagt gagacgcaga
     9361 agctcacggc cagcgacggt gttgcggacg accggctcgg aaactcggtc ggaatttcgg
     9421 gtcccgtcat cgtcgtcggg gctcccgacg taaccgtaca cggcaacgcc tttgcgggcg
     9481 ccgcgtacgt attcacccga tcggacgacg gcagctggac tcagagcgcc aagctggtcg
     9541 cgaacgatgg cgcggcgcaa gatcaacttg gcgcctcggt cgcagtctcc ggctcgacca
     9601 tccttgcggg ggcggaactc gccaccatcg acaacaaggc ggggcaggga gccacctacg
     9661 tgttcaccgg ttcgggtgat tcctggaacc aggttcaaaa aatcaccgcg gacgatggcg
     9721 gaccgggcga ttatttcggc gcctccgtcg ccatagcggg ctccgccgca atcgtgggcg
     9781 ccacccagga aggctggaac gggccggggg tcgcgtacgt gttcgagaac tcgggcggaa
     9841 cctggaacca gacggggaag ctcaccgacg ccaatggggt gtcgggagat gtattcggcc
     9901 tgggagtcgc gatttcgggt tcgaccgccg tcgtgggcgc cccgtttgtg accgtccatg
     9961 gcaatcctga acagggggcc gccttcgtat tctccgactc cggaggaagc tggacccaga
    10021 cccaggagct gttcgacgcc gatggcgggc agtatcgcgg cgaaaacttc ggctgggatg
    10081 tggcgctttc ggattcgacg atgctgatcg gatcgtattt cgcacagacg gttttcgagt
    10141 acacggaatc gaacggtacc tggacgctgg cccagaaact cagtcccagc gatgcgacgg
    10201 cggacgaggt gttcggaccg acggtagcgc tctcggggac ggattcgctt gtgggagcgc
    10261 agattgacac ggtcaacggc aacgacggcc agggagcggc ctacttctac ggagcggccg
    10321 atctgggatt gaccctcagc gcatccggaa cggtggggca aggacagaac tacgtgagtc
    10381 agacgatcgc gaccaacaac gcgagcgcgg catctccggc agtgagcgcg acgatctcgg
    10441 tgccggcggc ggcgagtttc gtcgcggcga gcgcatcgca gggcagttgc agcgaagact
    10501 cgggcgtcgt gagctgcgac ttcggggcga tcgcgggcaa cgccggcacg gcgacggcca
    10561 atgtgacact caaggccaca ggcaacgtcg gcgacacgat cgagaacacg gcgagtgtcg
    10621 ccaaggcgac gccggcgctg agcgcccgtg cgccgaccga gatcacggga tcggagagct
    10681 gtcccgacgg ctacacgagc tacagcggga cgctcgtccc gggagcgaac gtccgcattc
    10741 cgccctacag cgcagcggcg ggcgaagaga atgcgattct ttacgcgccc gagggcttcc
    10801 ggctctatgc gcgggtggat gcgaccctct ggggtcgcta ccaacgcggc ttcccggcaa
    10861 ccgaggtaca tcgccacggc ccggcgggcg agtaccgttg gctcgttcgc ggcaacgatg
    10921 gcggcggcga ctatctcttc tgcctgatgc atccctgagg cggctgcttt ctgccgccaa
    10981 tcggtctcgc gagggagcga ttgcgcacat cggcggcgcg caagcttcgc ggtgtttcgc
    11041 cagcctgccc cgaccttcgg ccgggagccg ccgcgcaggg ccccaaaggc cgccgggaca
    11101 ggcccgcggc ctgtttcccg ccattgactt ggagggtgct tccggcgata atacccagct
    11161 tgctcctgcg gaggggtacc cgagtggcca aagggggcag actgtaaatc tgctggctca
    11221 cgcctacgta ggttcgaatc ctaccccctc caccggctga agcggtaccc gctcgagcgt
    11281 gacgcctcgg atcggcaatg tccaggattg aagatgctgc ggaagctgat ttttgctgta
    11341 ggtgtcgcac cgcgggtgta gttcaatggt agaaccccag ccttccaagc tgatgacgtg
    11401 ggttcgattc ccatcacccg ctccagcgtg gacgatggtg cgataggccc acatagctca
    11461 gtcggtagag cacttccttg gtaaggaaga ggtcgccggt tcgattccgg ctgtgggctc
    11521 cagaaagaag tgagtgagaa agtgagattc aggaagtgat gcagtggcgg tttcggagtg
    11581 gtgctctttc tgccttgccc ggcagagcgc aggtttttga gcaaaagcag gcattgagga
    11641 gatagcagcg atggcgaagg cgaaattcga gcgtacgaag ccgcacgtga acgtggggac
    11701 gatcgggcat gtggaccacg gcaagacgac gtt
//
LOCUS       DASUJG010000235         2491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_70120, whole genome shotgun sequence.
ACCESSION   DASUJG010000235 DASUJG010000000
VERSION     DASUJG010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70120"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VFX38_05075"
     CDS             complement(<1..644)
                     /locus_tag="VFX38_05075"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5314261.1"
                     /translation="MIKLDANESPFALPEALRPVLAAALAKVELNRYPDGSAETLQRA
                     LREALGLPDSMGLVLGNGSDELLQLITTMVAKPGAVVLAPEPTFVMYAIYASHSAIRY
                     VGVPLNRELELDVAAMHDAIAREHPALVWLASPNNPTGGRVPVAGIESILQAAPGLVV
                     VDEAYCAYADESMLARATDFPNLVVTRTLSKTGLAGLRLGLAAGHLAWTRAIETL"
     gene            complement(876..2228)
                     /gene="hisD"
                     /locus_tag="VFX38_05080"
                     /pseudo
     CDS             complement(876..2228)
                     /gene="hisD"
                     /locus_tag="VFX38_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289112.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
     gene            complement(2243..>2491)
                     /gene="hisG"
                     /locus_tag="VFX38_05085"
     CDS             complement(2243..>2491)
                     /gene="hisG"
                     /locus_tag="VFX38_05085"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070642.1"
                     /note="short form of enzyme; requires HisZ for function;
                     catalyzes the formation of N'-5'-phosphoribosyl-ATP from
                     phosphoribosyl pyrophosphate; crucial role in histidine
                     biosynthesis; forms heteromultimer of HisG and HisZ;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX5314262.1"
                     /translation="LIKLYGSMELAPLAGLADAIVDLVSTGNTLKANDLVAVEDIMPI
                     SSRLIVNPAAIKLRRADVKPVHDAIEAAALAPAASASS"
BASE COUNT          381 a          843 c          868 g          399 t
ORIGIN      
        1 agcgtctcga tcgcgcgcgt ccacgccagg tgaccggcgg caagccccag ccgaagaccg
       61 gcgaggcccg ttttcgagag cgtgcgcgtg acgacgagat tcggaaaatc cgtggcccgc
      121 gcgagcatcg actcgtcggc atatgcgcag tacgcctcgt cgacgacgac gagaccgggc
      181 gccgcctgca ggatgctttc gatgccggca accggcaccc gcccgccggt cgggttgttg
      241 ggcgacgcga gccagaccag tgccgggtgt tcgcgcgcga ttgcgtcgtg catcgccgca
      301 acgtcgagtt cgagctcacg attgagcggc acaccaacgt agcgaatcgc cgagtggctt
      361 gcatagatcg cgtacatcac gaaggtcggt tccggcgcca gcacgacggc gcccggcttt
      421 gccaccatcg tcgtgatgag ctgaaggagc tcatcggagc cgttgccgag cacgagcccc
      481 atcgaatccg gcagtccgag tgcttcgcgc agcgcgcgct gcagagtctc tgcgctcccg
      541 tccggatagc ggttgagctc gaccttcgcg agcgctgcgg cgagcacggg ccgcaaggct
      601 tccggaagcg cgaacgggct ttcgttcgcg tcaagcttga tcatcgatcc ggccttcgcc
      661 acgacatagg gcgcgagtgc ctggaggtcc gggcgcacgc ttcgcgcggc gcgaaccgct
      721 gcctcgcgcg caatggcgac cgggtcccgc tcgcccgcgg tggcccgctc tttcgttgcc
      781 gatcgccgtt ccggagtgtg ttccccttgc gcaacgcgtt gccccgatgc tgccccactc
      841 gtcgccacgg ccccggtccg ggcttcggcc ctcgcttcgg cccgggcttc ggccgatctg
      901 gcgtgcgcga tcaggctctc accccgcgca agcgttgccg cgacgccggc gagcacgcct
      961 gcagtcgccg gcgcgatctt caggatgctg ctgcgcttct ggaagtcgta gacgccgagc
     1021 ggcgacgaga accgcgcggt gccaccggtc ggcaggacgt ggttgggtcc ggcgcagtag
     1081 tcgccaaggg cttcgcaggc gtggtgccca cagaagatcg ctccggcgtg acggatcttc
     1141 gggaggagcg cgtccgggtc ggccaccgcc agctccaggt gctccggagc gatccggttc
     1201 acgatcgcgc aggcttcgtc gaggtcgcgt acgcgaatga gcgccccgcg gcgcgcgaac
     1261 gatgccgaga tgatcgcggc gcgcggcatt tctgagaact ggcgatgcag gcttgccgcg
     1321 acgcgatcga tcagcgaggc gtcaggggtg acgaaaagcg cctgcgcgag ctcgtcgtgc
     1381 tccgcctgcg aaaagaggtc gagtgcgacc cagtcgggat cggcggtcga gtccgcgagg
     1441 acgagaatct ccgacgcccc ggccaccatg tcgataccga cgactccgaa tacgcgtcgt
     1501 ttggcggcgg cgacgtacgc attgcccgga ccgcagattt tgtcgactgc gggaatcgtg
     1561 gcggtcccgt aggcgagcgc cgcgatcgcc tgcgcaccgc cgattgcaaa ggcgcggttg
     1621 acgccagcga ggtgcgcggc tgcgagcacc aggggattgc gctcgccgcc ggcggtcggc
     1681 acgaccatca cgatttcgcg caccccggcg acctgagccg ggatcgcgtt catcagcacc
     1741 gacgacgggt aagccgcttt cccgcccggt acgtacagtc cggcgcggtc gagcggggtc
     1801 aatcgctgcc cgagctcggt accctcggcg tcgcgaagcg caaaatcgcc gccgcgctga
     1861 tggacatgga aatcacggat ccgacccgcg gcgaccgcga gtgcgttgcg cagccgttcc
     1921 ggcaacgcgt cttgcgcggc gcgcatctcg ccggccgtta tggcgagctc ggacgccgag
     1981 ccagccttcc agccgtcgaa gcgcgccgtg tattcgatca gcgccgtgtc gccgcggcgg
     2041 cggacgtcgg cgatgatcgc ggcgacgtca gcatcgatcg ccggatcctg cgcggcttcg
     2101 aaagcggtca ggcgcgacag cgccgcgtcg aaatcggccg cggtcgaatc gagccggcgc
     2161 agttgcagcg ccggcgacgg gttcgccggc gcttcgtgcg tcgttatcgc tgcattgcgc
     2221 gtcgtcatcg ctgcgtcgtt cgtcatgacg aggcggatgc ggccggcgca agcgccgccg
     2281 cctcgatcgc atcgtgcacg ggcttcacgt cggcgcgccg gagcttgatc gccgccggat
     2341 tcacgatcag ccgcgagctg atcggcatga tgtcctcgac agcaacgaga tcgttggctt
     2401 tcagcgtgtt tcccgtcgag acgaggtcga caatcgcatc ggcgagcccg gcaagcgggg
     2461 cgagttccat cgatccgtag agcttgatga g
//
LOCUS       DASUJG010000236         2309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1292649, whole genome shotgun sequence.
ACCESSION   DASUJG010000236 DASUJG010000000
VERSION     DASUJG010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292649"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VFX38_05090"
     CDS             complement(<1..656)
                     /locus_tag="VFX38_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006986266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX5314263.1"
                     /translation="MSELFLYNSLTRKKERLEEIRPGKIGMYVCGITVYDDAHLGHAR
                     MLLVFDTVVRYLRARGFEVRYVRNITDIDDKIIRRAVENGEDWRGLAERFAGRLAEDE
                     RALDLLRPDVEPRATDNIAAIVAMIAGLLEGGHAYVAANGDVYYSVASFAAYGALAGQ
                     RPEELRAGARIAPDEAKRDPLDFALWKAAKPGEPAWDAPWGSGRPGWHIECSVMSVAN
                     L"
     gene            complement(649..2031)
                     /gene="gltX"
                     /locus_tag="VFX38_05095"
     CDS             complement(649..2031)
                     /gene="gltX"
                     /locus_tag="VFX38_05095"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019628533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX5314264.1"
                     /translation="MIRTRFAPSPTGRLHLGNARTALFNALHAARTGGVFLLRIEDTD
                     AARSVATHAEALLDDLRWLGIEWSEGPDIGGRFGPYRQSERGEIHAESCARLLAEDHA
                     YPCFCKPEELAAQRSAALAAGRPPRYPGTCAAIAPAEARRRLAAGEAASLRFRIARQA
                     PVAFDDLVRGPQRLAADELGDFVIRRSDGSPAFLFANALDDAFMEITDVLRGEDHLAN
                     TPRQLLILEALALPAPRYGHLPLVVDASGKPLSKREAGISLAELRGAGILPEALANYL
                     FRLGHACARGELLDAEERVEAFAIAHLGRAPAQYDPAQLAHWQRLAIPALDPDAALAW
                     YGSLPVAGGRWAEFWNLLRGNVASRDDLALWAKTLATPPDRELVVAVGEAFRDAAAAL
                     CAEPDYRKFAAELRTRTGLGGRELYAPLRLALTGRHDGPELARLWVWFRAEERCARLR
                     RASEPERRHV"
BASE COUNT          385 a          795 c          777 g          352 t
ORIGIN      
        1 agattcgcga cggacatcac cgagcattcg atgtgccagc ccgggcgccc gctcccccag
       61 ggcgcatccc aggccggctc ccccggcttg gccgccttcc agagcgcgaa gtcgagcgga
      121 tcgcgcttgg cttcgtcggg agcgatgcgg gcgcccgcgc gcaactcttc cgggcgctgg
      181 cccgcgagcg caccataagc cgcaaagctc gcgacggagt aatacacgtc gccgttggcc
      241 gcgacgtacg catggccgcc ttcgagaagg cctgcgatca tcgccacgat ggcggcgatg
      301 ttatccgtcg cccgcggctc gacgtcggga cggagaagat cgagcgcgcg ctcgtcttcc
      361 gcgaggcgcc cggcgaaacg ttcggcgaga ccccgccagt cttcgccgtt ttccacagcg
      421 cggcggatga tcttgtcgtc gatgtcggtg atgttgcgca cgtagcgcac ttcgaaaccg
      481 cgcgctcgca gataccgcac cacggtatcg aagacgagca gcatgcgcgc gtggccgaga
      541 tgcgcgtcgt catagaccgt gatcccgcag acatacatgc cgatcttccc cggacggatc
      601 tcctcgagac gttccttttt gcgcgtgagc gagttgtaca agaacagctc agacatggcg
      661 gcgttccggt tcgctcgcgc gccgcagccg tgcgcaacgt tcctccgcgc ggaaccagac
      721 ccacagccgc gcgagttccg ggccgtcgtg ccgaccggtg agcgcgagtc gcaacggggc
      781 gtagagttcg cgcccgccga gccccgtgcg cgtccgcagt tcggctgcga atttgcggta
      841 gtcgggctcg gcgcagagcg cggcggccgc atctcgaaat gcttcgccga cggcgacgac
      901 aagttcgcga tcgggtggag tcgcaagcgt cttcgcccac agtgcgagat cgtcgcgact
      961 tgcgacattg ccgcgcaaca gattccagaa ctcggcccag cgcccccccg caaccggtag
     1021 cgacccgtac cacgcaagcg ccgcgtccgg atcgagcgca gggatcgcaa ggcgctgcca
     1081 atgcgcgagt tgcgccggat cgtactgcgc cggcgcgcgt ccgagatgcg cgatcgcaaa
     1141 cgcctccacg cgctcctccg cgtccaggag ttcgcctcgc gcgcaggcgt ggccgagacg
     1201 aaagagataa ttcgcaagcg cctcgggaag aatgcccgcg ccccgcaact cggcaaggct
     1261 gatgccggcc tcgcgcttgg aaagcggctt gccggaggca tcgacgacga gcggcagatg
     1321 accgtagcgc ggcgccggca acgcgagcgc ctcgaggatc aacaactggc gcggcgtatt
     1381 cgcgagatga tcctcgccgc gaagcacgtc ggtgatttcc atgaacgcgt catcgagcgc
     1441 gttggcgaaa agaaacgccg gcgagccgtc gctgcggcga atgacgaagt ccccgagttc
     1501 gtcggccgcg agacgctgcg gtccgcgcac gaggtcgtcg aaggcgacgg gcgcctgccg
     1561 ggcgatgcgg aaccgcagcg aggccgcctc gcccgccgcg agccggcgtc gggcttcggc
     1621 cggcgcgatc gcggcgcagg ttcccggata acgcggcggg cgtccggcgg ccagtgcggc
     1681 ggagcgttgc gccgcaagct cttcgggctt gcagaagcag ggataggcgt gatcttccgc
     1741 gagcagccgc gcgcaggatt cggcatggat ctcgcctcgt tcgctctggc gatacgggcc
     1801 gaagcgcccg ccgatgtcgg gcccctccga ccattcgatt cccagccagc gaagatcgtc
     1861 gagaagcgcc tcggcatgcg tggcaacgct tcgagcggca tcggtatcct cgatgcggag
     1921 caggaagacg ccgccggtcc tcgcggcgtg gagcgcgttg aacagcgccg tgcgcgcatt
     1981 tcccaaatgc aggcggcccg tcggactagg ggcgaagcgg gtacgaatca tgacaaggga
     2041 tccgtcggaa tcggctggcg gccgacgcgc gaattgcaca tttttgcaca gcgtcggccg
     2101 cggccggcgc ccgcaacggt atcatggacg caaatcgccg ctcgccccag ccttcgatac
     2161 acatgttccc gagactcctt gccgcctgcg tttttctcgt tcccgctgcg ggctttggag
     2221 cctcggctgc ggctcccgcc ccatccggct ccgccgcgac tccacgcgtc gaaatcgaaa
     2281 ccagcatggg cgacatcgtg gtcgcgctc
//
LOCUS       DASUJG010000237         3053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_802830, whole genome shotgun sequence.
ACCESSION   DASUJG010000237 DASUJG010000000
VERSION     DASUJG010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802830"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2520)
                     /locus_tag="VFX38_05100"
     CDS             complement(<1..2520)
                     /locus_tag="VFX38_05100"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX5314265.1"
                     /translation="MNQFLAIVMGCAVFAAGGWNQALGGTAVPASAEAPGASTQTQAV
                     PTGRLPRDVVPLAYTLDFTMNPDANGYRAHEVIDIRITKPLATIWLHGQGIKVTDATL
                     ALADGKTLKIAYKQEDDSGVAKFSLPHTVPAQKAQIVLDFTNPYSQGLMGVYKVEAGG
                     DNYIFTQFEAIAARRAFVSFDEPGFKTPFTYSFTVPKSDKVVANTPVRSKSDAGSGMV
                     KWQFMTTRPLPTYLVAWAVGPLDIVAGPPMPPNEVRDRAVPLYGVTVKGKGEEIRYAL
                     AHAGEIVAAEEKYYGIAYPWRKLSLIAVPDFGAGAMENAGAITFRDTLLLMDPKTAPT
                     WQKRAYWSVAAHELAHMWTGDLVTVPWWNDIWLNEAFATWMAQKVMDELHPAWNSRLG
                     AISSRQAAMRADSLASARAVRQPIKSTGDIKNAFDGITYLKGAAVISMFEHYVGAEKF
                     REGMHDYLQQNAYGSGSLDDFLAAISKAAGENVGPAFKTFLNQPGLPMVAATLEMKNG
                     KPVVHLVQSRYLPIGSTGNREGEHWDIPVCMSYAAGGRSQSQCYMLTSHSADVPLAAG
                     AMPGWFLPNADGDGYFQWSLDKGGYAALAGALDRLSPADQMSFAGSIEAAFNAATIDT
                     ATTMRTLAALAHAKSYPVAEEPMDLVTFARERLTGAQAKAGAEAFARKLYGGYDVAAD
                     FARGGAPANDNRREFEASVADFLAFTGQDKAVRGAADAAADRVLGLDDHGKTDFAAVA
                     PDFLPIALSVAVRDQGKPVFDALEKILRNTSDPLIRSVTLDAMASATEPALAQRARAM
                     VFEPGLLRKNEILAVVFIQMGQEQTRDATWEWLTANYEKLLK"
     gene            2794..>3053
                     /locus_tag="VFX38_05105"
     CDS             2794..>3053
                     /locus_tag="VFX38_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314266.1"
                     /translation="MNGKTESACEATGWIDPFTMAGLFPLSAPIVSTRLIDCADAERL
                     AASAQTLAVVRHGSARAAGDAAAPCLDTGLELLAGPACELWET"
BASE COUNT          520 a         1007 c          961 g          565 t
ORIGIN      
        1 cttgagaagc ttttcgtagt tcgcggtgag ccactcccag gtcgcgtcgc gagtttgctc
       61 ctggcccatc tggataaaga ccacggcaag aatttcgttc ttgcggagca ggccgggttc
      121 gaacaccatc gcgcgcgcac gttgcgcgag cgccggctct gtcgcactcg ccatggcgtc
      181 cagcgtcacg ctgcggatga gcgggtccga ggtgttcctc aagattttct cgagggcatc
      241 gaacacgggc ttgccctgat cgcgcacggc gacgctcaac gcgatcggca gaaaatccgg
      301 cgccacggcg gcgaagtccg ttttgccgtg atcgtcgagt ccgagcacgc gatccgccgc
      361 ggcgtccgcc gctccgcgca ccgccttgtc ctgtccggtg aaggcaagga agtcggcgac
      421 gctcgcctcg aactcgcggc gattgtcgtt tgcaggcgcg ccgccccggg cgaagtcggc
      481 ggcgacgtcg tagccgccat agagcttgcg cgcgaacgct tcggctcccg ccttcgcttg
      541 tgcgccggtg agccgctcgc gtgcgaaagt gacgagatcc atcggttcct cggcaacggg
      601 gtaactcttc gcatgcgcca gcgcggcgag cgtgcgcatg gtggtcgccg tgtcgatggt
      661 cgcggcattg aacgccgcct cgatgctgcc cgcgaatgac atctggtccg ccgggctcag
      721 ccgatcgagc gcgccggcaa gcgccgcgta cccgcccttg tcgagcgacc actgaaaata
      781 gccgtcgcca tcggcgttcg gcaggaacca gcccggcatc gcgcccgcag cgagcggcac
      841 gtccgcgctg tgcgacgtca acatgtagca ctgactctgc gacctgccgc cggcggcgta
      901 actcatgcac acgggaatat cccaatgctc gccctcgcga ttgcccgtcg atccgatcgg
      961 aaggtagcgg gactgcacga gatgcacgac aggcttgcca tttttcatct cgagcgtcgc
     1021 agccaccatc ggcagtcccg gctgattgag gaaggtcttg aacgccgggc cgacgttctc
     1081 tcccgcggcc ttggaaatgg cggcgaggaa gtcatccagg ctgccgctcc cgtaggcgtt
     1141 ctgttgcaga tagtcgtgca tgccttcgcg aaacttctcc gcgccgacgt agtgttcgaa
     1201 catgctgatg acggccgcgc ccttgagata ggtgatgccg tcgaaggcgt tctttatgtc
     1261 gccggtactc ttgatgggct ggcgcactgc gcgggcggag gcgagggaat cggcccgcat
     1321 cgcggcctgc cggctcgaga tcgcgccgag gcgcgaattc cacgcgggat gcagctcgtc
     1381 catgaccttc tgcgccatcc aggtagcgaa ggcctcgttc agccagatgt cgttccacca
     1441 gggcacggtt acgagatcgc ccgtccacat gtgcgcgagt tcgtgcgcgg cgacgctcca
     1501 gtacgcgcgc ttctgccagg tgggcgccgt cttcggatcc atgagcagca gggtgtcacg
     1561 aaaggtgatg gcgcctgcgt tctccatcgc gcccgcgccg aagtccggca ccgcgatcag
     1621 cgaaagtttg cgccagggat aggcgatgcc gtagtacttt tcctccgccg ccacgatctc
     1681 ccccgcgtgc gcgagcgcgt agcggatttc ctcgcccttg cctttcacgg tgacgccgta
     1741 cagcggcacc gcacgatcgc gcacttcgtt cggcggcatc ggcggcccgg caacgatgtc
     1801 aagcggtccg acggcccagg cgacgagata ggtcggcaac ggcctggtgg tcataaactg
     1861 ccacttcacc attccgctgc cggcatcgct cttcgacctg accggagtgt tcgcgacgac
     1921 cttgtcgctc ttcggcacgg taaaactgta ggtaaacgga gtcttgaagc caggctcgtc
     1981 gaaacttacg aaggcgcgcc gcgcggcgat cgcttcgaac tgcgtgaaaa tgtaattgtc
     2041 gccgccggcc tcgaccttgt agacacccat gagcccctgc gaatagggat tggtgaagtc
     2101 gagcacgatc tgcgcctttt gcgccggcac cgtgtgcggc aggctgaatt tcgcgacgcc
     2161 gctatcatct tcctgcttgt aggcaatctt gagcgttttc ccgtcggcaa gcgcgagcgt
     2221 cgcatccgtc accttgatgc cctgaccgtg cagccagatg gtcgcgagcg gcttcgtgat
     2281 ccggatgtcg atgacctcgt gcgcacggta gccattcgcg tccggattca tggtgaagtc
     2341 gagcgtgtag gcgagcggca cgacatcgcg cggcaaccgc cccgtgggca cggcctgcgt
     2401 ctgcgtcgac gcgcccggcg cttccgcgct cgcgggcacc gccgtgcctc ccagagcctg
     2461 attccagcca ccggcggcga acaccgcgca acccatgacg atcgcaagaa actggttcat
     2521 gcgcacgttc tttctccggc cgaaaacata ttcgcacttt agcagcttgt cccagatcgg
     2581 caaacgaagg catctccctt gatccccctt cgcaagcgag gaaggagaac gatggactga
     2641 cctctatcgg cgccgccaga aaacacggtt atcgccccct tcggaacgaa gggggtcgcg
     2701 tcttcgcggg gggatgttct gcgccagaac cggaaagaat tcccctcgat cccccttcgc
     2761 aagcgaaggg ggaaactgga gagcacttcg cgcgtgaatg ggaagacaga gagtgcctgc
     2821 gaagcgacgg gctggatcga tccttttaca atggccggac ttttcccctt gagcgcaccc
     2881 atcgtatcta cacgcctgat tgattgcgcc gacgccgaac gccttgccgc atcggcgcaa
     2941 acgcttgccg tcgttcgaca cggctcggcg cgcgccgccg gcgacgccgc cgcgccctgc
     3001 ctcgacaccg ggctggaatt gctggcgggc cccgcctgcg aactgtggga gac
//
LOCUS       DASUJG010000238         9344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_681504, whole genome shotgun sequence.
ACCESSION   DASUJG010000238 DASUJG010000000
VERSION     DASUJG010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9344
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681504"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="VFX38_05110"
     CDS             <1..960
                     /locus_tag="VFX38_05110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HEX5314267.1"
                     /translation="VCAVVNMADDGGSSGGLRRDFHIPALGDLRNCLLATAEGDPTLQ
                     ALFDFRFKTGVDGHSLGNLMLAALLFLERDLAKAVARANVLLLAHARVLPATTANVEL
                     LAEFADGHMVRGETRIVAEGRPIRKLSLLPGDAEPTAEVLAALAASDLIVLGPGSLYT
                     SVIPNLLLKGMAAAIARSGALVALVMNLMTEPGETRGYRGRDVIAALRQHAPALPIHA
                     VVVNDAPLPPGLRERYAARGSVPIAVEPVLLEGMGYRCRSLPLLAQGVKIRHDPVKLA
                     ESLLVLAGAARADSGARALLGARREFASPPSPLAVRAGRSVGP"
     gene            957..2231
                     /locus_tag="VFX38_05115"
     CDS             957..2231
                     /locus_tag="VFX38_05115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5314268.1"
                     /translation="MNFLQGADSAGAAAASEVAPMPPYAPPSSDRLRLLTFMTLFGIG
                     GTEKQVVNLSKLLDRERFALRFGCLKRWGELLEEVEREGFVIDEYPIMRLYGREAMRQ
                     QWRFARALARDRVQIMHSYNFYANVFSIPAARMARVPCVVASVRDLGVYLTPAQCRVQ
                     NWACRLADVVLVNAEAIRRWMIAQGFPEKKLRVIRNGVDLAKFRVKADGSLRRELELP
                     PQAPVVVMLARLDPKKGVEYFLRAAPRIMERCPDTYFVVVGEAFLRDPDDERRPDLEQ
                     RRELERRAAELGIGEHIRFVGMRPDVPEILAMATVSVLPSFSEGLSNTLLESMAAGVA
                     VVATDVGGTPEVIRNGCDGILVPPANTRAIVDAVCSILEDPLLAARLRAQGRERVASA
                     FSFDKMTRATEAVYDELMQRKRRGNHQQVLGA"
     gene            2246..3466
                     /locus_tag="VFX38_05120"
     CDS             2246..3466
                     /locus_tag="VFX38_05120"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5314269.1"
                     /translation="MFIREQEGATVARLMQTPAAPLAAGTSVQEIDCGEALASLRDPW
                     SELLRASASNGIFLTWEWLVHWWQQLGHVRGRLRVLVVRRAGRIIAIAPLIERERQPG
                     RLWPFRTLEFMGMGSVGSDYLDFLVRAGEEAPAVAALTDRLARERTVFEFRRVAAGSP
                     MVLRVARRLEERGWTVTEGVEEACPYVRLEGHDWESYLASLGSAHRQNLRRCLRRIER
                     DFEPVFEEVETEAQRSACLKVFENLHRQRWDARGGSDALTGEAVMRFHDTFSRLALER
                     GWLRLSVLRLSGRPAAAVYAFCYANVVHYYQAGFDPAFRRYSVGLAAMGLGIRAAIAQ
                     GLGEYDLLHGAEAYKSLWARNRRELLCIRCFPPGLRGFVPYRTERLREVLKALRRRVP
                     GPARQPSSATGARP"
     gene            3463..4542
                     /locus_tag="VFX38_05125"
     CDS             3463..4542
                     /locus_tag="VFX38_05125"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX5314270.1"
                     /translation="MKLRTVAKTAGSYALYHSGALRLNERLRGGEPGPVVVGLHRVVK
                     SFRESSKGHMAPMLTSSSRLERYLNWMGRRRRFVSLEEAVANDVAGSASPVPLAAITF
                     DDGYRDVYYNAFPLLRRKRIPFAVFVVSDLVGTERLQVHDELYLLCRRALRRWRESAR
                     PKLEPLLTDSGCGQVLSTWDGDAMKLTRAILHALPQDDVLHLLEKLRRVLGDVAEESS
                     HLRPLTWQMLEEMHAAGVTVGSHGCNHALLTQERAQRVASELRGSKTKLEEHLDSEVR
                     YFAYPDGKFNTAVVAAVAEAGYEAAFTTCPHRDPAHPALTVPRRLLWENACVSARGRP
                     SPAVLECLLGGAFDVADKCLDNHDA"
     gene            4622..6553
                     /gene="asnB"
                     /locus_tag="VFX38_05130"
     CDS             4622..6553
                     /gene="asnB"
                     /locus_tag="VFX38_05130"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX5314271.1"
                     /translation="MCGIAGIVGRGAGDVALEELRAMAQVLRHRGPDEEGFYLGDTAG
                     LAIRRLRVIDLATGRQPMRSEDGAIAIVLNGEIYNYLELRRELEHRGHRFRSRSDTET
                     VLRAYEAWGLDCVRKLRGMFAFALWDERRKTLHIARDRLGIKPLYYGEIAGRFVFASE
                     LKAILQLPALRRSLNPDALDHLLATLTTPATESIVAGVHKLEPAHTLSLAPGRAPEIR
                     RYWSVEFEPDRTHDAAYFADGLRELLAESVRLHCLSDVPLGAFLSGGVDSSAIVALMA
                     RTSAKPVKTFSIGFENGAFDERDHARRVAQEFGTEHHELVLDADIEPLLERLAWHLDE
                     PFGDSSAIPTYAVSELAAREVTVVLSGDGGDELFAGYDKYGVEERERRRERLPALLRR
                     GLGRVGSALPEGARGRNFLRHLGLSGMERYLDAGALFDRAQRDFLVLPEARARNRGDE
                     QTLERGLDGSRLHWLSRLQQLDLGEYLPLDILTKVDRMSMAHSLEARVPLLDHKLVEF
                     AATIPPELQRRNGESKHIFKTAMRGILPEATITRRKQGFAVPLGTWFRGRLTSMLHEL
                     LLGETASARGIFDRRYVERLIAMHERGRGLDLELWTLMSFELWCRIVFDRAPPAMARD
                     AAAPQLVPSPPAEGLRARA"
     gene            6614..8827
                     /locus_tag="VFX38_05135"
     CDS             6614..8827
                     /locus_tag="VFX38_05135"
                     /inference="COORDINATES: protein motif:HMM:NF028199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEX5314272.1"
                     /translation="MRLQRVLHAGLPVLANRGWQELRRSADRMTSEFATSRRNSVPAA
                     LRRGAFAIRPANPGSLAATQPLTSETSFPEPNPQFAARLLGERFPEFRERILAEADAA
                     CERRLRLLGREFRFAGGFDWHRDPASGRRAPLLHWSRLDPLNALQVGDSKFVWELNRH
                     QWLVGLATAWRLTANERYAQAVASALQDWMRANPPGIGINWASSLEVSLRLIAWCWIL
                     HLLAGSRSVTAELRRRMREQIHVHATHVERYMSHFFSPNTHLTGEALGLVYAGTLLAG
                     LPRAARWRRVGARVLLRELERQVLPDGVHFEQSTCYARYTAEIYLHFCLLDERAGTKL
                     AQATKPRLCKLLDYLLAIRRPDGSLPPIGDDDGGRLLPLAPRAPDDCRELFALAAVLF
                     SRADYAWAAGGPAPEIAWFLGREGWESFAALPPAPPDAAPSQIFSAGGYAVMRGSWDR
                     DAHQLVFDAGPLGCPYSGGHGHADLLSIQCSPFGHAQLSDPGTYCYTAAPAWRKHFRG
                     SRAHSTVVVDGESQALAAGPFSWASRPRARLRRWISTPQFDLADGEHEAYARLRDPVS
                     HRRRVLYVKPRYWIVVDDLDGWKSHRIELLLQFTAASVVSNADGWWQAADGDGWGLFV
                     RAFAPVALAAHIERGATAPIAGWESADYGQRTPAPLLVHAVEALLPLRIVTVLLPRAP
                     RNRAPEALFAANRLSLRFPEGRESLRFEERAIRIERDGDAAVSLPLAGHPNSPER"
     gene            8860..>9344
                     /locus_tag="VFX38_05140"
     CDS             8860..>9344
                     /locus_tag="VFX38_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HEX5314273.1"
                     /translation="MDGGRESDRSRLERMGAALAHRGPDDGGIFIGSGAALVHRRLSI
                     IDVAGGHQPMTNTAGDIWITCNGEIYNYRELRRELRVRGREFSTSSDTEVILRAYETF
                     GEDCVRYLRGMFAFAIWDARRRRLFLARDRLGIKPLYYAQTGNELLFASEIKALLAAG
                     I"
BASE COUNT         1418 a         3129 c         3115 g         1682 t
ORIGIN      
        1 gtatgcgccg tcgtcaacat ggccgacgac ggcggcagct cgggcggcct gcgccgcgac
       61 tttcacattc cggcgctggg cgacctgcgc aattgtcttc tcgccaccgc cgagggagac
      121 ccgacgctgc aggcgctgtt cgattttcgt ttcaagaccg gcgtcgacgg gcattcgctc
      181 ggcaatctga tgctcgcggc gctcctgttt ctcgaacggg atctcgcgaa ggccgtggca
      241 cgcgcgaacg tcctgctgct cgcccatgcg cgcgtgcttc ccgccaccac cgccaacgtg
      301 gagcttcttg ccgaattcgc cgacggacac atggtccgcg gggaaacgag gatcgtcgcc
      361 gaaggccgcc cgattcgcaa actgtcgctg cttccgggag acgccgagcc gactgccgag
      421 gtgctcgcgg cgctcgccgc ttccgatctc atcgtgctcg ggccgggaag cctctatacc
      481 agcgtgattc ccaatctgct gctgaaggga atggcggcgg cgatcgcgcg atcgggagcg
      541 ctcgttgcgc tcgtcatgaa cctgatgacc gagcccggcg aaacgcgagg ctatcgcggg
      601 cgcgacgtga tcgcggcctt gcggcagcac gcgccggccc tgccgattca tgcggttgtc
      661 gtcaacgatg cgccgcttcc cccgggactg cgcgagcgtt acgcggcgag gggttccgtt
      721 ccgatcgcgg tcgagccggt cctgctcgaa ggcatgggat accgttgccg cagccttccg
      781 cttctcgcgc agggcgtgaa gatccgccac gatcccgtga agctcgcgga gtccttgctg
      841 gtgctcgcgg gcgctgcgcg cgcagacagc ggcgcgcgag cgctgctcgg agcgcgacgc
      901 gaattcgcaa gcccgccgtc gccgcttgcc gtgcgtgcgg gccggagcgt cggcccgtga
      961 attttctgca gggcgccgat tccgcgggtg cggccgccgc ttccgaagtc gcaccgatgc
     1021 cgccgtacgc gccgccgtct tccgatcgac tgcggctcct caccttcatg acgctcttcg
     1081 gaatcggagg caccgaaaag caggtcgtga acctgtcgaa gctgctcgat cgcgagcgct
     1141 tcgcgctgcg attcggctgt ctcaagcgct ggggcgaatt gctggaggaa gtggaacgcg
     1201 aaggtttcgt catcgacgaa tatccgatca tgcgtctcta cggccgcgaa gcgatgcgcc
     1261 agcaatggcg attcgcccgc gccctcgcgc gcgatcgcgt tcagatcatg cacagctaca
     1321 acttctatgc gaacgtgttc agcattcccg ccgcgcgaat ggcgcgcgtt ccctgcgttg
     1381 tcgcctccgt gcgcgatctc ggcgtttatc tcacgcccgc gcagtgccgg gtgcagaatt
     1441 gggcctgccg gctcgccgac gtggtgctcg tcaacgccga ggccatccgc cgctggatga
     1501 tcgcgcaggg tttcccggag aaaaagctgc gggtcatccg caacggcgtg gacctggcga
     1561 agttccgggt gaaggccgac ggctcgctgc gccgggaact ggagcttccg ccgcaggcgc
     1621 cggtggtggt catgctcgcg cggcttgatc cgaagaaggg cgtcgagtat tttctaaggg
     1681 cggcgccgcg gatcatggag cgctgtcccg acacctattt cgtcgtcgtc ggcgaggcct
     1741 ttcttcgcga tcccgacgac gagcgccgcc ccgatctcga gcagcgccgc gaactggaac
     1801 ggcgtgcggc cgaactcggg atcggcgagc acatccgctt cgtcggcatg cgccccgacg
     1861 tgccggaaat tctcgcgatg gcgacggtct cggttctgcc ctccttcagc gaaggcctct
     1921 ccaatacgct gctcgaatcc atggcggcgg gcgtggccgt cgtcgcgacc gacgtgggcg
     1981 gcacgccgga ggtgattcga aacggctgtg acggcattct cgtccctccg gcaaacaccc
     2041 gggcgatcgt cgatgccgtc tgcagcatcc tcgaggatcc gctgctcgcc gcgcgcctcc
     2101 gcgcgcaggg acgcgagcgg gtcgcgagcg ccttctcctt cgacaagatg acgcgcgcga
     2161 ccgaggccgt ctatgacgaa ctcatgcagc ggaagcgccg cggaaatcac caacaggtac
     2221 tcggcgcatg aagtcgggag cgtcaatgtt cattcgcgaa caggaaggcg ctaccgttgc
     2281 acggttaatg cagacgcccg cagcgccgct cgcggcgggc acgagcgtgc aggaaatcga
     2341 ttgcggcgag gcgctcgcat ccctgcgcga tccctggagc gagcttttgc gggcgagcgc
     2401 ctccaatgga atttttctca cctgggagtg gctcgttcac tggtggcagc aactcgggca
     2461 cgttcggggc cggctgcggg tgctcgtcgt gcgccgcgcc gggcgaatca ttgcaatcgc
     2521 gcccctgatc gagcgcgaac ggcagccggg acggctgtgg ccgtttcgta cgctcgaatt
     2581 catgggcatg ggaagcgtcg gctcggacta tctcgatttt ctcgttcgcg ccggcgagga
     2641 agccccggca gtcgcggcgc tcaccgatcg ccttgcgcgc gagcgcaccg tcttcgagtt
     2701 ccgccgcgtt gctgccggct cgccgatggt gcttcgcgtc gcccgcaggc tcgaagaacg
     2761 cggatggacg gttacggaag gcgtcgagga agcctgtccc tatgtccggc tcgagggtca
     2821 cgactgggag tcgtatctcg ccagtctcgg aagcgcgcac cgccagaatc tgcgccgctg
     2881 cctgcgccgc atcgagcgcg acttcgagcc ggtgttcgag gaggtggaaa cggaagcgca
     2941 gaggagcgct tgtctgaagg tgttcgagaa tctgcatcgg cagcggtggg acgcgcgcgg
     3001 cggttcggac gcgctcaccg gcgaagcggt aatgcggttt cacgacacat tcagccgcct
     3061 cgccctcgag cgcggctggc tgcggctctc cgtgctgcgt ctgagcgggc ggccagccgc
     3121 cgccgtgtat gcgttttgct atgcgaacgt ggtccattac taccaagctg gcttcgatcc
     3181 cgcattccgg cgttacagcg tggggctggc ggcgatgggg ctcggcattc gggccgcgat
     3241 cgcgcagggt ctcggcgaat acgatttgct ccacggcgca gaagcgtaca agtcgctttg
     3301 ggcccgaaac cggcgcgagc tgctttgcat ccgctgcttt ccacccggcc tgcgcggctt
     3361 cgttccttat cggaccgagc gcctgcggga agttctcaag gcgctgcgtc gccgagttcc
     3421 gggcccggcg cggcagccat cgtccgcaac cggggcgcgg ccatgaagct gcgtacagtg
     3481 gcgaagacgg ccggctccta cgcgctctat cactcgggcg cgttgcggct caacgagcgc
     3541 ctgcgcggcg gcgagccggg tcccgtcgtg gtcggtttgc atcgcgtcgt gaagagcttt
     3601 cgcgaaagtt cgaagggcca tatggcgccg atgctgacca gcagctcgcg gctggagcgc
     3661 tatctcaact ggatggggcg gcggcggcgt ttcgtgagcc tcgaggaagc ggtcgcaaac
     3721 gatgtcgcgg gaagcgcctc gccggttccg ctcgcggcca taacctttga cgacgggtat
     3781 cgcgacgtct actacaacgc ctttcccttg ttgcggcgca agcgcattcc cttcgccgtc
     3841 ttcgtcgttt ccgatcttgt gggcaccgag cggctgcagg ttcacgacga gctgtacctc
     3901 ctctgccgac gcgcgttgcg ccgctggagg gaatcggctc gcccgaagct ggagcctttg
     3961 ctcacggatt cgggttgcgg gcaggtcctg tccacttggg atggcgacgc catgaagctg
     4021 acgcgcgcga tccttcatgc cctgccgcag gacgacgttc tgcacctgct cgagaaactt
     4081 cgccgggttc tgggcgacgt ggccgaggag tcctcgcatt tgcgaccgct cacctggcaa
     4141 atgctcgagg agatgcacgc cgccggggta accgtcggct cgcacggctg caaccatgcg
     4201 ctgcttacgc aggaacgcgc gcagcgcgtc gcgagcgaat tgcgcggatc gaaaacgaaa
     4261 ctcgaggaac atctggactc cgaagtccgg tacttcgcct atcccgacgg gaaattcaac
     4321 actgcggtgg tcgcggcggt cgccgaggcc ggttacgagg ccgcgttcac gacctgcccc
     4381 catcgcgacc ccgcgcatcc ggcgctcacc gtgccgcgcc ggcttctctg ggaaaatgcc
     4441 tgtgtgagcg cgcgcgggcg gccctcgccg gccgtgctcg aatgcctcct cggcggcgcc
     4501 ttcgacgtcg ccgacaagtg cctggacaac catgacgcct gagcacgcag cgtcgccacc
     4561 gcgccggctt cgatcacgat ggcagtcgcg gccgatgccc cgggagcatc ggggctaggc
     4621 cgtgtgcgga atcgcgggaa tcgtcggtcg cggagcgggc gatgtggcgc tcgaggaact
     4681 gcgcgcgatg gcgcaggttc tgcgtcatcg cggccccgac gaagagggct tctatcttgg
     4741 cgacacggcg ggactcgcga tccgccgcct gcgcgtgatc gatctcgcga ccggacgcca
     4801 gccgatgcgg agcgaggacg gcgccatcgc catcgtcctg aacggcgaga tctacaacta
     4861 cctggaactg cgccgcgaac tcgagcaccg gggccatcgt tttcgcagcc gctccgacac
     4921 cgaaaccgtg ctccgtgcct acgaggcctg gggtctcgac tgcgtgcgca agctccgtgg
     4981 catgttcgca ttcgcgctct gggacgagag gcgcaaaacg ctccacatcg cgcgcgatcg
     5041 tctcgggatc aagccgcttt attacggcga gatcgcaggc aggttcgtct tcgcctccga
     5101 attgaaagcc atcctgcaat tgccggctct gcggcgaagc ctgaatccgg atgccctgga
     5161 tcatctcctt gcgaccctca cgacgcccgc gaccgaaagc atcgtcgccg gcgtgcacaa
     5221 gctcgaaccg gctcacacgc tgagcctcgc tcccggacgg gcgccggaga tccggcgcta
     5281 ctggagcgtc gagttcgagc ccgaccgcac gcatgatgcg gcgtatttcg ccgacggctt
     5341 gcgcgagttg ctcgcggagt cggtgcggct ccactgcctg agcgacgtgc cgctcggcgc
     5401 ctttctcagc ggcggcgtgg actccagcgc gatcgtagcg ttgatggcgc gcacgagtgc
     5461 gaagcccgtg aagacctttt ccatcggttt tgaaaacggc gcttttgacg aacgcgatca
     5521 tgcgcgccgg gtggcgcagg aattcggtac cgagcatcac gaactggtgc tcgacgcgga
     5581 tatcgagccc ttgctggagc gtcttgcctg gcatctcgat gagcctttcg gagattcctc
     5641 cgcgattccc acgtacgccg tttccgaact cgccgcgcgc gaagtcacgg tggtgctttc
     5701 cggcgacggc ggcgatgagc tttttgccgg ctacgacaag tacggcgtgg aagagcgcga
     5761 acgccgccgc gaacgcctgc cggcattgtt gcgccgcggt ctcggacgcg tcggatcggc
     5821 gctgccagag ggagcgcggg ggcgcaattt tctgcgccat ctgggtttga gcggcatgga
     5881 acggtatctc gatgccgggg cacttttcga tcgcgcgcag cgggatttcc ttgttctccc
     5941 cgaggcgcgc gcgcgcaatc gcggcgacga gcaaacactc gagcgcggtc tcgatggcag
     6001 tcggctccat tggctgtcgc gcctgcagca actggatctc ggggaatatc tgccgctcga
     6061 cattctcacg aaggtggatc gcatgagcat ggcgcattcg ctcgaagcgc gggtgccgct
     6121 gctcgatcac aagctcgtgg aattcgccgc caccattccg cccgaactgc agcgtcgcaa
     6181 cggagagtcg aagcacattt tcaagactgc tatgcggggg atcctgcccg aagcgaccat
     6241 cacgcgccgc aagcagggat tcgccgttcc cttgggaaca tggttccggg ggcgcctgac
     6301 ttccatgctc cacgaactgc tgctcggaga aaccgcgagc gcacgcggaa ttttcgaccg
     6361 acgttacgtc gagcgcctga tcgcgatgca tgagcgcggc cgcggcctcg atctcgaact
     6421 ctggacgctg atgtctttcg agttgtggtg ccgaattgtt ttcgatcgcg cgccgccggc
     6481 gatggcgcgg gacgcggccg cgccccaact cgtccccagc ccgcccgccg aaggattgcg
     6541 ggcgcgtgcc tgacgtgcgt acgcgagtag cgccgcgacg cccgacgggc gcttgccggg
     6601 aaggcgcaac gccgtgaggc tgcagcgggt actgcatgcg ggcctgccgg tgcttgccaa
     6661 tcggggatgg caggaactgc gccgctccgc cgatcgcatg acgagcgagt tcgcgacgtc
     6721 ccgacggaac tccgtacctg ccgccttgcg gcgcggcgca ttcgcgatcc gacccgcgaa
     6781 tccaggttcg ctggcggcta cgcagccgct gacctcagag acttcctttc ccgagccgaa
     6841 tccgcaattc gcggcgcgtc tgctcggcga gcgtttcccc gaatttcgcg aacgcatcct
     6901 cgccgaggcg gacgcggcct gcgagcggcg cctgcgcctc ctcggccgcg agttccgctt
     6961 cgccggcggc ttcgattggc atcgcgatcc cgcctccggt cgccgcgcgc cgctcctcca
     7021 ttggagccgc ctcgatccgc tgaatgcgct gcaggtcggc gacagcaaat tcgtctggga
     7081 gctgaaccgc caccaatggc tggtgggtct cgccaccgcc tggcgcctga cggccaacga
     7141 gcgctatgcg caggccgtcg cgagtgccct gcaggactgg atgcgcgcga acccgccggg
     7201 aatcgggatc aactgggcga gcagcctcga ggtttcgctc cggctcatcg cctggtgctg
     7261 gatcctccat ctgctcgccg gatcgcgcag cgtcactgcc gaactgcgcc gccggatgcg
     7321 cgagcagatt catgtgcacg cgacgcatgt ggagcgctac atgtcgcatt ttttctctcc
     7381 caatacccat ctcacgggcg aggcgctcgg cctcgtgtat gcgggcacgc tgctcgcggg
     7441 tctgccgcgg gcggcgcgct ggcgccgcgt cggtgcgcgg gtgttgctgc gcgaactcga
     7501 gcgccaggtt cttcccgacg gcgtgcattt cgaacagtcc acctgctacg cccgctacac
     7561 cgccgagatc tatctgcact tttgcctgct cgacgaacgc gccggaacaa agctggcgca
     7621 ggcgacgaaa ccgcgtcttt gcaaattgct cgactacctc ctcgcgattc gtcgccccga
     7681 cggctcgctg ccgccgatcg gcgacgacga cggcggccgg ttgctgccgc tcgcgccgcg
     7741 ggcacccgac gactgtcgcg aactcttcgc gctcgccgcc gtgctgttct cgcgcgccga
     7801 ctatgcctgg gcggccggag gtccggcgcc ggagatcgcg tggttcctcg gccgcgaggg
     7861 ctgggagagt tttgccgcgc tcccgcccgc accgccggat gcggccccct cgcagatatt
     7921 ttccgcgggg gggtacgccg tgatgcgcgg cagttgggat cgtgatgcgc atcagctcgt
     7981 cttcgacgcc ggtccgctcg gctgtcccta cagcggcggt cacgggcatg ccgatctcct
     8041 gagcatccag tgctctccct tcggtcatgc gcaactgagc gatccgggaa cgtattgcta
     8101 caccgcggcg cccgcgtggc gcaagcattt tcgcggcagc cgcgcgcaca gcactgtcgt
     8161 cgtcgacgga gaaagccagg cccttgccgc cgggccgttt tcctgggcaa gccgtccgcg
     8221 cgcccggctg cggcgttgga tttcgacgcc acagttcgat ctcgccgacg gcgaacacga
     8281 ggcgtacgcg cgcctgcgcg atccggtgtc gcatcggcgc cgggttctct atgtcaaacc
     8341 gcgctactgg atcgtggtgg acgatctcga cggctggaaa agccaccgga tcgaactgct
     8401 gctgcagttc accgccgcga gcgtggtttc gaacgcggac ggctggtggc aggccgcgga
     8461 tggtgacggg tgggggttgt tcgtgcgcgc cttcgcgccg gtcgcgctcg cggcgcacat
     8521 cgagcggggg gcgacggcgc cgattgccgg ctgggagtct gccgattacg ggcaacgaac
     8581 tccggcgccg ctcctcgtgc acgccgtcga ggccctgctg ccgttgcgga tcgtgaccgt
     8641 gcttctcccg cgcgccccgc gcaaccgcgc cccggaggcg ctcttcgcgg ccaatcgtct
     8701 cagcttgcgc tttccggaag gccgcgaatc gctccggttc gaagagcgcg ccatccgcat
     8761 cgaacgcgac ggcgacgcgg ccgtatccct gcccctggcc gggcacccga attctcccga
     8821 gaggtaatac ccatgtgtgg aatggctgga atcatatcat tggacggtgg ccgtgaatcc
     8881 gatcggtcgc gactcgagcg catgggcgcc gctctcgcgc atcgcggtcc ggatgacggc
     8941 ggaattttca tcggctccgg ggcggcgctc gtgcatcgcc gcctctcgat catcgatgtc
     9001 gccggaggcc atcagccgat gaccaatacc gcaggcgaca tctggatcac ttgcaacggg
     9061 gaaatctaca actatcgcga gttgcgccgc gaacttcgcg ttcgcggccg cgaattctcc
     9121 acctcctccg acacggaagt gatcctgcgc gcctacgaga cattcggcga agactgcgtg
     9181 cgctacctgc gcgggatgtt cgccttcgcg atctgggatg cgcgccgccg caggcttttt
     9241 ctcgctcgcg accgtctcgg gatcaaaccg ctctattacg cgcaaacggg gaacgaactg
     9301 ctgttcgcgt cggaaatcaa ggcgcttctc gcggcgggga ttga
//
LOCUS       DASUJG010000239         3341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_70831, whole genome shotgun sequence.
ACCESSION   DASUJG010000239 DASUJG010000000
VERSION     DASUJG010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70831"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="VFX38_05145"
     CDS             <1..920
                     /locus_tag="VFX38_05145"
                     /inference="COORDINATES: protein motif:HMM:NF040246.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RelA/SpoT AH/RIS domain-containing protein"
                     /protein_id="HEX5314274.1"
                     /translation="EIIQLPRGATPVDFAYAVHTDVGNACVSAKVDRRVVPLRMPLAT
                     GQTVEIITARGAHPNPAWLNFVVSAKARSALRRYLRELEAEDAVRFGRRMLEQVLVGY
                     RIKLQRESERLATVAEIFKARDSQDLLRRIGIGEFPAALVARHFLGDAESAPGESRAS
                     AAPVEIRGTEGLAVTLARCCHPIPGDRIVGLLTRGKGLVVHRLDCRNLAAQKDLDRVV
                     DIAFDDSRQRDYTIELRLEVENSRGVLAAVAATISTADSNIRHAQVIEREDQTSSLVL
                     ELVVHDRAHLARIMRHLRKMPAVLRLARS"
     gene            complement(924..2267)
                     /locus_tag="VFX38_05150"
     CDS             complement(924..2267)
                     /locus_tag="VFX38_05150"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5314275.1"
                     /translation="MKKPAHESRLALLALLGLVPALGLFAAAPWLGLALWAHAVLVAG
                     AALAGLAAAFAIARHSRRASRRLASLVLGIRDSEFGLRARAEAGAFGETLAALNALTE
                     ELAGMRRAGIESDALLGKLLSAVELAILVFDPRGRLVGVNRAGETLFGADADGLYGRS
                     ATALEVADWLAADAPLRATKTLPAGDGPWEVRVARFRRAGREHRLLTVTDMSAALREE
                     ERRAWRGLIRVLGHETSNSLGPIQATADALRRRLVAAALGGELAAALGGGLELIERRA
                     RALAAFIHRYAELARLPPPNLERVRLAELVPRVAALEDRTRVGAHGAEDLVVLADPGQ
                     LEQVLINLIKNAADAVQTTGGAVDVSWRREREMAVIEVRDEGAGLPRTENLFVPFFTT
                     KPGGSGIGLVLARQIAEAHGGSLALANRRDRDGAVASLHLRLTDDAQASLAARQS"
     gene            complement(2413..>3341)
                     /locus_tag="VFX38_05155"
     CDS             complement(2413..>3341)
                     /locus_tag="VFX38_05155"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5314276.1"
                     /translation="NADEGGIIFRSAAMREMMATVAKIVDADVAVLLTGESGTGKGLL
                     ARAIHERSSRAARPFVHVNLGALPETLAESELFGHVKGAFTDARADKPGRFELADGGT
                     LFLDEISNATGAMQARLLTALESGSVERLGATRSRSVDVRIVSASNADLGAMIARDAF
                     RSDLYFRLNTVEIRIPSLRERPEDIPVLAAHFLTRFARHHGKPLEGFARTAEAALLGY
                     DWPGNVRELAHVVERAVLLAAGTRIEVGDLRLPGETTGEATTFEAMSLEQAERYLLQK
                     ALARNDGDAAAAARQLGVSRSAFYRRLAKLKQ"
BASE COUNT          532 a         1124 c         1154 g          531 t
ORIGIN      
        1 gcgagatcat ccagttgccg cgcggcgcga ctcctgtgga tttcgcctac gccgtgcata
       61 ccgacgtcgg caacgcctgc gtctccgcca aggtcgaccg gagagtcgtg ccgctgcgca
      121 tgccgctcgc gaccgggcag acggtggaga tcatcacggc gcgcggcgcg catcccaatc
      181 ccgcctggct caacttcgtg gtgagcgcca aggcgcgctc ggcgctgcgg cggtatctgc
      241 gcgaactcga agccgaagac gcggtgcgct tcgggcgacg aatgctcgag caggttctcg
      301 tgggctaccg catcaagctg cagcgcgaga gcgagcgcct cgcgacggtg gcggagatct
      361 tcaaggcgcg cgattcccag gatctgttgc gccgcatcgg catcggcgag tttccggcgg
      421 cgctcgtcgc gcgccatttt ctcggcgacg ccgagtccgc gccgggtgag tcccgggcga
      481 gcgccgcacc cgtcgagatc cgcggaaccg agggcctcgc cgtcacgctc gcgcgctgct
      541 gccatcccat tccgggcgat cgcatcgtcg ggctcctcac ccgcggcaag ggcctcgtag
      601 tgcaccggct cgattgtcgc aatctcgcgg cgcagaagga tctcgaccgg gtcgtggaca
      661 ttgccttcga cgattcgcgt cagcgcgact acacgatcga gcttcgtctg gaggtggaaa
      721 attcgcgcgg cgtgctcgcg gccgttgccg ccacgatctc gaccgcggat tcgaacatcc
      781 gccacgcgca ggtgatcgag cgggaagatc agacttcgag tcttgtcctc gaactcgtcg
      841 tgcacgatcg cgcgcatctg gcgcggatca tgcgacatct gcgcaaaatg ccggcggtgc
      901 tgcggctcgc ccgaagttga aagtcagctc tggcgagcgg cgagcgatgc ctgcgcgtcg
      961 tccgtcagcc gcagatgcag ggacgcgacg gcaccgtcgc gatcgcggcg gtttgcgagc
     1021 gcaagcgagc cgccgtgcgc ttcggcgatc tggcgggcga gtacgaggcc gatgccgctt
     1081 ccgccgggct tggtcgtgaa aaacggcacg aagagattct ccgtgcgtgg caggccggcg
     1141 ccctcgtcgc gcacctcgat cacggccatc tcgcgctcgc gccgccagct tacgtccacc
     1201 gcgccgccgg tcgtctgaac ggcgtcggct gcattcttga tgagattgat gaggacctgt
     1261 tcgagctgcc ccggatcggc cagtacgacc aggtcttccg ctccatgcgc gccgacacgg
     1321 gtgcgatcct ccagcgccgc gacgcgcggc acgagttcgg cgagacgcac ccgctcgagg
     1381 ttcggcggcg gcagccgcgc aagttcggcg tagcgatgga tgaacgcggc gagcgcgcgc
     1441 gcccgccgct cgatgagttc gagtccgcct ccgagcgccg ccgcgagttc tccgccgagc
     1501 gcggcggcga cgaggcggcg gcgcagtgcg tcggccgtcg cctgaatggg gccgagagaa
     1561 ttgctggttt cgtggccgag gacacggatg aggccgcgcc aggcgcgccg ctcttcttcg
     1621 cgcagtgcgg ccgacatgtc ggtcaccgtg agaaggcgat gttcgcggcc cgcacggcga
     1681 aagcgtgcga cccgaacctc ccagggcccg tcgcccgcgg gcagggtttt cgttgcgcgc
     1741 agcggagcat ccgcagcgag ccaatcggcg acttcgagcg ctgtggcact gcgcccgtaa
     1801 agcccatcgg cgtcggcccc gaacagggtt tcgccggcac gattgactcc gaccagacgc
     1861 ccgcgcggat cgaataccag aattgcgagt tcgaccgcgg aaagcagctt tccgagcagc
     1921 gcgtccgatt cgatgcccgc gcggcgcatg cccgcgagtt cctcggtgag cgcgttgagc
     1981 gcggcgagcg tttccccgaa tgcgccggcc tcggcacggg cgcggagtcc gaattcgctg
     2041 tcgcggatgc cgagcacgag gctcgcgagc cgacgcgaag cgcggcgcga atgccgggcg
     2101 atcgcgaagg cggcggcaag ccccgcgagc gcggcgcccg caacgagcac ggcatgtgcc
     2161 cagagcgcga gaccgagcca gggcgcggcg gcgaagaggc cgagagccgg caccagaccg
     2221 aggagcgcga ggagcgcgag ccgcgattcg tgcgccggtt ttttcatgca gcattacgct
     2281 gtgtatcgcc gccccggcga aggccgggac ccgacaggaa catgtatctc gcccaacggg
     2341 cgggaatcat tatcggctga ataccggcct tcgccggcaa gatctgcccc gaagtgtgcc
     2401 ggcagcaagc attcattgct tgagtttggc gagtcgtcgg tagaaggcgc tgcgcgacac
     2461 gccgagctgg cgcgctgccg cggccgcatc gccgtcgttg cgcgcgagcg ccttctgcag
     2521 aagatagcgt tcggcctgtt cgaggctcat cgcctcgaac gtcgtcgctt cgcccgtcgt
     2581 ctcccccggc agacgtagat cgcccacctc gatgcgcgtt cccgcggcaa gcagcaccgc
     2641 gcgttcgacg acatgtgcga gttcgcgcac gtttcccggc cagtcgtagc cgagaagcgc
     2701 cgcctccgcg gtgcgggcga aaccttcgag cggctttccg tgatggcgcg cgaagcgcgt
     2761 gagaaaatgc gccgcgagta ccggaatgtc ttccggccgt tcgcgcaggg aggggatgcg
     2821 aatctccacc gtattcagcc gaaagtacag gtcgctgcgg aatgcatcac gcgcgatcat
     2881 cgcgccgaga tcggcattgc ttgccgagac gatgcgaaca tcgacgctgc gcgagcgcgt
     2941 ggcgccgagc cgttcgaccg atccgctttc gagtgccgta aggagccgcg cctgcatggc
     3001 gcccgttgcg ttcgaaatct cgtcgagaaa aagcgtgccg ccgtcggcga gctcgaagcg
     3061 cccgggcttg tcggctcgcg cgtcggtgaa cgcgcccttg acgtggccga agagttcgct
     3121 ttcggcgagc gtttccggca gtgcgccgag attgacatgc acaaaaggtc gcgcggcacg
     3181 cgacgaacgc tcgtgaatcg cgcgcgcgag tagccccttg ccggtaccgc tttcgccagt
     3241 gagcaggacg gcgacatcgg cgtcgacgat cttggccacg gtcgccatca tctcgcgcat
     3301 cgccgccgaa cgaaagatga ttccgccttc gtccgcgttg t
//
LOCUS       DASUJG010000240         7045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_316000, whole genome shotgun sequence.
ACCESSION   DASUJG010000240 DASUJG010000000
VERSION     DASUJG010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7045
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316000"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VFX38_05160"
     CDS             <1..763
                     /locus_tag="VFX38_05160"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX5314277.1"
                     /translation="DALNKRDPDIIGAGLADAGAAAISLDGGPMMPAITAPSGSPPQV
                     APNTAVTFAGICGYDGIRSLDYDWFFGTSEIPNSHKRDPDPVEYAYGGVYTVQFFCSD
                     DLQRAEADLVLDVEAPATAEDLSFSVVQNQRFTGQLKGSGLGGGSLIYEATTLPGHGT
                     LTIHGPGPGYDYIPDKDYVGDDSFQYYIDNGVMKSNVATVSISVKAAPPPPPPPPPPP
                     TGGGGGGSLGAAVLGLLAGLALLLRRRRWTRMRIK"
     gene            complement(791..1408)
                     /gene="yihA"
                     /locus_tag="VFX38_05165"
     CDS             complement(791..1408)
                     /gene="yihA"
                     /locus_tag="VFX38_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017639244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEX5314278.1"
                     /translation="MQFSQARFLLSAAGPRDMPADAGREVAFAGRSNAGKSSALNRLA
                     SRRGLARTSATPGRTQLVNFFALDDKDERRLVDLPGYGYARAPQALRNQWMALVERYL
                     GERRSLSGLVLLADSRLPLKPEDEALIDWARASRLPLLLLLTKADKLGRAAQAAALAR
                     AYQRLVSGETALVFSAVSGLSVETARDWIANRLEMPQAAQDSGSA"
     gene            1478..2146
                     /locus_tag="VFX38_05170"
     CDS             1478..2146
                     /locus_tag="VFX38_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX5314279.1"
                     /translation="MPAKVLRLASLLTIALLAAPLLAAAPPAPASAAAAAVRIDRVVP
                     AQPSGAGPDQIGVLVFFDFSPASQALMNYLEGWSARAGRHVALDREPLVTAAAAPLAR
                     AFVVARTLGVIEPVLPALFKLAAKEDPAAAPPTAMAVKGVFAPWGIDPIEFHAAWRSP
                     VATDGVTRARALAYRFGVTTAPVIIVNGVWRLTPAPGTKPRKLIGALDARIAAAATLE
                     AENQ"
     gene            2143..2616
                     /locus_tag="VFX38_05175"
     CDS             2143..2616
                     /locus_tag="VFX38_05175"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX5314280.1"
                     /translation="MKDSPVAIQDLYPEDFAHCYGCGRLNAHGYRIKSHEQGDVLVAH
                     FDPEPWQLSVPGFVYGGLIASLIDCHAMAAAAAATCRAEGREPGSEPALRFVTASLKV
                     DYLKPTPLGPLELRARAREVGARKVRLDVELLAAGEITARGDVLAVRIPISMVKP"
     gene            2619..4220
                     /locus_tag="VFX38_05180"
     CDS             2619..4220
                     /locus_tag="VFX38_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5314281.1"
                     /translation="MEHISYAYGENHWARDPDLPAVLARYWPQWRSAKEDLETFGALA
                     GREVYETCYHVDHEAVPVLVSHDVNGRRVDRVRLAPSERDLLHRLAPVMRPPYEGGSW
                     HHHYALGYLIADPGLFCVLTITQQTVYAIHKYAPEHAAWKEKLLAGEAFGATWMTENQ
                     GGSDLGANRARALNENGGWRIYDGDKYFASGAGLADVAITTARPEGAPTGPKGLALFL
                     LPRLDSRGELNFRVGRLKDKSATRAVPSGEVELNGAEAWLIGKADEGIYYTLENLTVS
                     RLTNATAAMGIARKAAFEVAGRVENRHSFGKALVEHPLVRRDLTDIAVRLAGGTALAF
                     RAVAAFDAAWQERPPYSSAYHAARFWSHLAKARTAEHSAAVTQLAMELFGGLGFLEEY
                     AVARWHREALITPIWEGPANIQALDMLEAMQKKHAHESFVAEMHELLERAGTEEADLA
                     RDALDAALAAQTAAADRAGAEWIAKRTLTRLADVASVALLYDLAASGGERYARLAGLY
                     ARRFLAGEEYPAATHFDRDIFRLTA"
     gene            4243..5184
                     /locus_tag="VFX38_05185"
     CDS             4243..5184
                     /locus_tag="VFX38_05185"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX5314282.1"
                     /translation="MPKGFQDFLAKFDYAALLQNAIRIAIIILVAIVLWFIIRFVISR
                     VAKRMVDRAVANGEGTNAATQRAQTLSSLIRKLVAALYWVAVVLTLLSQIGVNIGALV
                     AGAGIAGLALSFGAQSLVKDYISGFFIVLENQISVGDVATLNGTGGVVEEINFRTIVL
                     RGGDGSVHVFRNGDITQLQNQTRGWSGYMFEIGVAYKEDTDRVIATIERIGREMKEDR
                     QLGASMIQELEIFGVNQLGDSAVVITGRLKTQPGAHWGVGRAFLARVKKTFDAAGIEL
                     PFPQQTLWFGAGGAPLRITRAEQATAKEKQSDPDAPA"
     gene            complement(5186..6022)
                     /locus_tag="VFX38_05190"
     CDS             complement(5186..6022)
                     /locus_tag="VFX38_05190"
                     /inference="COORDINATES: protein motif:HMM:NF039293.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HEX5314283.1"
                     /translation="MNGDAGSAAALWARARDLHEAGVRKGAIHAYRQRVTTIAAGGIV
                     WVLRVLENAAEPAPRGADPFLPPYDPDQHVADLGRHHVLLLNKYPVLPAHVLIVTREA
                     LSQDAIPGPGDFAAATRLLDGPHTLLFYNGGPESGASQPHRHFQAVRLPLGPSSEAIP
                     TAPWIRDHLAGAALPFPGAALAIAAGAWSEEDGGAQLRTIAVELLRRCGRSAQAPGSF
                     NLLLTREWMLAVPRVARAWGGVPINSLAYAGALIARSQAAERTLRRNGPLAVLIGAAD
                     GR"
     gene            complement(6019..>7045)
                     /locus_tag="VFX38_05195"
     CDS             complement(6019..>7045)
                     /locus_tag="VFX38_05195"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5314284.1"
                     /translation="FASGKATPAELREKVRSGDLFAYFTIPADPLAADAPFVYASRNL
                     TDTDLRDWFAGEVTTVIQARKVASVGLPSAKADWLKAPVEFQSRLVTKEGARAATAAE
                     QAGQWLPIGYVYLLFIAIMSIAQLLMMSTIEEKSTRIAETLLATVEPTDVMAGKTLGV
                     AAVGTTLVCCWLAIILGLTAAFGGMLPIGGFAHALVAGITVWNVAWFLVYFILGFLLY
                     SAVLGAIGAAVNNIQEAQPFIGPVMMFMVLPLIIMVPVVKDPTATWARVLSYFPPLTP
                     FLMVNRSAAPPPLVDYVATTAILIVTVAFALYASGKIFRVGLLSTGAPPKLKELLAWL
                     RTPRPVQ"
BASE COUNT         1206 a         2335 c         2347 g         1157 t
ORIGIN      
        1 ggacgcgctg aacaaacgcg atcctgacat catcggcgcc gggctcgccg atgcgggagc
       61 tgccgccatt tccctggatg gcggcccgat gatgccggcc atcaccgcgc caagcggatc
      121 gccgccccaa gtggccccga acaccgcggt gacttttgcc ggaatctgtg gttatgacgg
      181 catccgctcc ctcgactacg actggttttt cggcacgtcg gaaatcccca actcgcataa
      241 acgcgaccct gatcccgtgg agtatgcgta cggcggcgtc tataccgtcc agtttttctg
      301 cagcgacgat cttcagaggg cagaggccga tctcgtcctg gatgtcgaag ccccggccac
      361 ggccgaggat ctcagcttca gcgttgtcca gaatcagcgg ttcacgggtc agctcaaggg
      421 cagcgggctc ggtgggggtt ccttgatcta cgaggccacg accctgcccg ggcacggtac
      481 gctgacgatt cacggtcctg gccccggcta tgactacatt cccgacaagg actacgtcgg
      541 cgacgacagc tttcagtact acattgataa tggcgtcatg aagtccaatg tcgcgactgt
      601 gtccatcagc gtcaaagcag cgccgccgcc accaccgccg ccaccgccgc cgccaacggg
      661 tggcggtggc ggcgggtcgc tcggcgcggc tgtgctcggc ctgctcgccg gcttggcctt
      721 gctcctgcgt cgtcgccgtt ggactcggat gcggatcaag taacggcgcg gccttgaccg
      781 gcgtgctgcg ttatgcggag ccggaatcct gcgcggcctg cggcatttcc agacgattgg
      841 cgatccagtc ccgggctgtc tccacgctca ggccggaaac ggcggaaaaa acgagcgccg
      901 tctcgccact cacaaggcgc tggtatgcgc gggcgagcgc cgccgcctgc gccgcgcgcc
      961 cgagcttgtc ggcctttgtg agcagcagca acagtggcag tctgcttgcg cgcgcccagt
     1021 cgatcagggc ctcgtcttcg ggcttgagcg ggagacgcga atcggcgaga agcacgaggc
     1081 ccgagagcga acgccgttcg ccgagatagc gttcgaccag cgccatccat tggtttcgca
     1141 gcgcctgcgg agcgcgggca taaccgtacc cgggcaagtc caccagccgg cgctcgtctt
     1201 tgtcgtcgag cgcgaagaaa ttgacgagct gcgtgcgccc cggagtcgcg ctggtgcggg
     1261 cgaggccgcg ccgggacgcg aggcggttga gcgcgctcga ctttccggca ttcgagcggc
     1321 cggcgaaggc cacttcgcgg ccggcgtcgg cgggcatgtc gcgcggtccc gccgcgctca
     1381 gaagaaatcg ggcctgggag aattgcatcg gtttccgtcg ggatcgactc cgccgtctag
     1441 cttaccgtcg ccgccgcggt gttgtactat cgctgtcatg ccagcgaaag tcctgcgcct
     1501 cgcatcgttg ctgacgattg cgctactcgc ggcgccgttg ctggcggcgg ccccgccggc
     1561 gcccgcgagc gcagctgctg cggcggtgcg gatcgatcgg gtcgtgcccg cccagccgag
     1621 cggcgctggc ccggatcaga tcggtgtcct ggtgtttttc gatttttcgc ctgcctcgca
     1681 ggcactcatg aattaccttg aagggtggag cgctcgtgcg ggccggcacg tggcgctcga
     1741 tcgcgaaccg ctcgtgaccg ccgccgccgc accgctcgcg cgcgccttcg tcgtcgcccg
     1801 caccctgggg gtgatcgaac cggtactgcc ggcgcttttc aaacttgccg cgaaggaaga
     1861 ccccgcagcg gcgccgccga cggcgatggc cgtaaaagga gtcttcgcac cgtggggaat
     1921 cgacccgatc gagtttcacg cggcctggcg ttcgccggtt gcgaccgacg gagtgactcg
     1981 cgcccgggcg ctcgcctacc ggttcggcgt cacgactgcg ccggtgatca tcgtcaacgg
     2041 cgtgtggcgg ctgaccccgg ccccgggaac gaagccgcgg aagctgattg gtgcactcga
     2101 tgcccggatc gcggccgccg cgacgctcga ggccgagaat cagtgaagga ctcgccggtc
     2161 gcaattcagg atctgtatcc cgaggacttc gcgcactgct atggctgcgg ccggctgaat
     2221 gcccatggct atcggatcaa gagccacgag cagggggacg ttctggttgc gcacttcgac
     2281 cccgagccct ggcaactgtc cgtccccggc ttcgtctatg gcggcctgat tgcctcgctc
     2341 atcgattgcc acgcaatggc cgcggccgca gccgcgacct gtcgcgccga aggccgcgag
     2401 ccgggttcgg agcctgcgct gcgcttcgtt acggcgagcc tcaaggtgga ttatctgaag
     2461 ccgacgccgc tcggcccgct cgaactgcgc gcccgcgccc gcgaggtcgg agcgcgcaag
     2521 gttcgcctgg atgtcgaact gctcgcggcg ggcgagatta ccgcccgcgg cgacgtgctc
     2581 gccgttcgta ttcccataag catggtcaag ccgtgagtct ggaacatatc agctacgcgt
     2641 acggcgaaaa ccactgggcg cgcgatcccg atctgcccgc cgtgctcgcc cggtactggc
     2701 cgcagtggcg cagcgcgaag gaggatctcg agacctttgg cgcccttgcc ggacgcgaag
     2761 tgtacgaaac ctgctatcac gtcgaccatg aagcggtgcc cgtgctcgtc tcgcatgatg
     2821 tgaacggccg gcgcgtcgat cgcgtgcgcc tcgcgccatc cgagcgcgat ctcctgcatc
     2881 ggctggcacc ggtgatgcgc cctccttacg agggtggaag ctggcaccac cattacgcgc
     2941 tcggatatct gatcgccgat cccggtcttt tctgtgtgct caccattacc cagcagaccg
     3001 tctacgcaat ccacaagtac gcgcccgaac acgcggcgtg gaaggaaaaa ctccttgcgg
     3061 gcgaggcctt cggcgcgacc tggatgaccg agaatcaggg aggttccgat ctcggcgcga
     3121 accgggcccg cgcgctgaac gagaacggcg gctggcgaat ttacgacggc gacaagtact
     3181 ttgcgagcgg cgcgggactc gccgacgtcg ccattaccac cgcccggccg gaaggcgcgc
     3241 cgacgggacc gaaggggctc gcactgttcc tgcttccgcg gctcgattcg cgcggggaac
     3301 tcaattttcg cgtcggccgc ctgaaggaca agagcgcaac ccgcgccgtg ccttccggtg
     3361 aggtcgaatt gaacggcgcc gaagcctggc tgatcggcaa ggccgacgag gggatctact
     3421 acaccctgga aaatctcacc gtatcgcgtc tcacgaacgc caccgcggcg atgggcatcg
     3481 cccgcaaggc ggccttcgaa gttgccggcc gcgtcgagaa tcgccacagc ttcggcaagg
     3541 ctctcgtcga acatccgctc gtgcgccgcg acctcaccga tatcgcggtg cgtcttgccg
     3601 gcggcacggc gctcgccttc cgcgcggtcg ccgccttcga cgccgcctgg caggagcgtc
     3661 cgccgtacag ttccgcctat catgcggcgc gcttctggtc gcatctcgcc aaggcccgca
     3721 ccgcggaaca ttccgccgcc gtgacccagc ttgcgatgga actcttcggc ggccttgggt
     3781 ttctcgaaga atatgccgtc gcgcgctggc atcgcgaagc cctgatcacg ccgatctggg
     3841 aagggcccgc gaacatccag gcgctcgaca tgctggaggc gatgcagaaa aaacacgcac
     3901 acgagagctt cgtcgccgaa atgcacgagc tgctggaacg cgccggaacg gaggaggccg
     3961 atctcgcccg cgatgcgctc gatgccgcgc ttgcggcgca gactgcggcc gccgatcgcg
     4021 cgggcgccga gtggatcgcc aagcgcacgc tcacgcggct cgccgacgtc gccagcgtcg
     4081 cgctgctcta cgatctcgcg gcgagtggcg gcgaacgcta cgcgaggctc gccggtcttt
     4141 atgcccggcg gtttctcgcc ggcgaggaat atcccgcggc aacgcatttc gatcgcgaca
     4201 tcttccgtct caccgcctga tccgcaggac agaggagcgc atatgccgaa aggattccag
     4261 gacttcctcg ccaaattcga ctacgcagcg ctcctccaga acgcgatccg gatcgcgatc
     4321 atcattctcg tcgccatcgt gctctggttc atcatccgtt tcgtgatctc gcgcgtcgcc
     4381 aaacgcatgg tggatcgcgc cgtcgccaac ggcgagggca cgaacgccgc cacccagcgc
     4441 gcgcagacgc tttcgtcgct catccgcaaa ctggtcgccg ccctgtactg ggttgccgtc
     4501 gttctgacac ttctctcgca gatcggcgtg aacatcggtg cactcgtggc cggcgccggc
     4561 atcgccggtc tcgcgctgtc cttcggcgca caaagtctgg tgaaggacta tatctcgggc
     4621 tttttcatcg tgctcgaaaa ccagatcagc gtcggcgacg tggccactct caacgggacg
     4681 ggaggcgtgg tcgaggagat caatttccgc accatcgtcc tgcgcggcgg cgacggttcg
     4741 gtgcacgtgt ttcgcaacgg cgacatcacg caactccaga atcagacccg gggctggagc
     4801 gggtatatgt tcgaaatcgg ggtcgcgtac aaggaggaca ccgatcgcgt gatcgcgacc
     4861 atcgagcgca tcgggcgcga aatgaaggaa gatcggcaac tcggcgcgag catgatccag
     4921 gaactcgaga tcttcggcgt caatcagttg ggcgattccg cggtggtcat caccggcagg
     4981 ctcaagaccc aacccggcgc gcattggggc gtcggacgtg cgttccttgc gcgagtgaag
     5041 aagaccttcg acgccgcggg catcgagctt ccgtttccgc agcagacgct ctggtttggc
     5101 gcgggcggcg cgccgctgcg aatcaccaga gcggagcaag caactgcgaa ggagaagcag
     5161 tcggatccgg acgctcccgc gtagattacc ggccgtcggc ggcgccgatg agcacggcga
     5221 gcgggccgtt ccggcgcagc gttcgctccg ccgcctgcga gcgcgcgatc aaggctcccg
     5281 cgtacgcgag tgagttgatc gggactccgc cccaggcgcg agcgacgcga ggcacggcga
     5341 gcatccattc gcgggtcagg agaagattga acgagccggg cgcctgcgcg ctgcgaccgc
     5401 agcgccgcag cagctccacg gcgatcgtcc gcaattgcgc gccgccgtcc tcttcgctcc
     5461 aggcgccggc cgcgatcgcg agtgccgcac ccggaaaggg aagggcggcg ccggcgagat
     5521 ggtcgcgaat ccatggcgcg gtcgggatcg cctcggacga gggcccgagc ggcagacgta
     5581 cggcctgaaa gtgacgatgc ggctgactcg cgccggactc cggcccgccg ttgtaaaaga
     5641 gcaaagtgtg cggcccgtcg agcagtctcg tcgcggcggc aaagtctccg gggccgggaa
     5701 tggcgtcctg tgacaaggct tcgcgcgtaa cgatgaggac atgcgccgga agcaccggat
     5761 atttgttgag aagaagcaca tgatggcgcc cgagatcggc cacgtgctgg tcgggatcgt
     5821 agggcggaag gaagggatcg gctccgcggg gcgcgggctc cgccgcattc tcgagcacgc
     5881 gcagcaccca gacgattccg ccggccgcga tggtggtgac ccgctggcgg tacgcatgaa
     5941 tcgcaccctt gcgcacgccc gcctcgtgca gatcgcgcgc gcgcgcccac agcgcggcgg
     6001 cgcttcccgc gtcgccgttc actgcaccgg gcggggagta cgcagccagg cgagcagttc
     6061 cttcagtttg ggcggggcgc cagtgctgag aagtccgacg cggaagatct ttccggacgc
     6121 atagagcgcg aatgccacgg tgacgatgag gatcgccgtc gtcgcgacgt agtccaccag
     6181 aggcggaggg gccgccgagc ggttgaccat caggaacggc gtgagcggcg gaaaatagga
     6241 cagcacccgc gcccaggtcg cggtcgggtc cttgacgacg ggcaccatga tgatgagcgg
     6301 caggaccatg aacatcatca ccggcccgat gaagggctgc gcctcctgga tgttgttgac
     6361 ggcggcgccg atcgcgccga gaacggccga atagagcaga aacccgagaa tgaaatacac
     6421 gagaaaccag gccacgttcc agaccgtgat ccccgcaacc aaggcgtgag cgaagcctcc
     6481 gatcggcagc attccgccga aggcggccgt gagtccgaga atgatcgcga gccagcaaca
     6541 cacgagagtc gtgcccacgg cggcgactcc gagagtcttg cccgccatca cgtcggtggg
     6601 ttcgaccgtc gcgagcaggg tttccgcgat ccgggtcgac ttctcctcga tggtggacat
     6661 catcaggagc tgggcgatcg acatgatcgc gatgaacagg agatagacat agccgatcgg
     6721 caaccactgc cccgcctgct cggccgcggt cgcggcgcgc gctccttctt tcgtcaccag
     6781 gcggctctga aactcgaccg gcgccttgag ccagtcggcc ttggcgctcg gcaacccgac
     6841 cgaggcgacc ttgcgggcct gaatgacggt cgtgacctcg cccgcgaacc agtcacgcag
     6901 atccgtgtcc gtgagattgc gcgaggcata gacgaagggc gcatcggccg cgagcggatc
     6961 ggccggaatg gtgaagtagg cgaagagatc gccgctgcga accttctcgc gcagttcggc
     7021 cggcgtcgcc ttgccggatg cgaaa
//
LOCUS       DASUJG010000241         2096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1415807, whole genome shotgun sequence.
ACCESSION   DASUJG010000241 DASUJG010000000
VERSION     DASUJG010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415807"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1911
                     /locus_tag="VFX38_05200"
     CDS             <1..1911
                     /locus_tag="VFX38_05200"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEX5314285.1"
                     /translation="YVGIIFSPFNDTQWGYEFFCSTGATEFDEFRQQGNEYSSFDAIW
                     YCKAGLTEDGYEVVMQIPFRSLKFPHSDRPQTWRMLFFRNWARTVRHQITQVPINYNS
                     NCLLCDSEIVRTATPIHSSGANLQIIPAATIARTDTRTRPSSGLVAGSPQISGGLDAR
                     WAIRPDLEWSATLNPDFSQVAPDVLQSTVNERFALFYPENRPFFEEGTWVFNTPGFNY
                     GEQDGNNQFVDTRQIADPHWASKVVGQIGSNALGALVADDSITNILMPGPVQSNLQSF
                     DFGTRDALLRYRQDFPGNSSLGVLATDREGGGYSNGLVGLDGSWQLDPSDSITAQLAY
                     TDTNYPDEVAFAFGVAPGRVTGNGWLVSFQRTRHNYTTSLLLARVAPGFRADLGYLPQ
                     VGYSEAMPQYEYDWYSSDSWWNNGGFGGSYDFVQATGDGPVLDRRAQVYAFAHAIGQS
                     HLIFFARHEDQYFGGETFALNQLEMDASAQPLSWLEAELDVTGGDDVDYIGVREGGLL
                     SVSPSFTLTPGRHLQIQFVGNFERLNVTGGRLYTANLYDLRVAWYFNSQMFVRAIAQE
                     QDVRNNTALYPPGTPSRERALATQWLFGYELNPWTSLFAGFTNNYLATGDVGLLQQGR
                     TYFVKMSYDFQL"
BASE COUNT          392 a          696 c          606 g          402 t
ORIGIN      
        1 tacgtcggga tcattttcag ccccttcaac gacacgcaat ggggctacga attcttctgc
       61 agcaccggcg cgacggaatt cgacgagttc cgccagcagg gcaacgaata ctcgtctttc
      121 gacgccatct ggtactgcaa ggccggcctg accgaagacg gctacgaggt cgtgatgcag
      181 attccgttcc gctcgctcaa gttcccgcac agcgaccggc cgcagacctg gcggatgctg
      241 ttctttcgca actgggcacg aacggtccgc caccagatta cccaggttcc gatcaattac
      301 aacagcaact gccttctgtg cgattccgag atcgtccgca ccgcgacgcc catccattcc
      361 agcggggcga atctgcagat cattccggcg gcgacgatcg cacgcaccga tacgcgcacg
      421 aggccgtctt ccggtctcgt cgcgggcagc ccgcagattt ccggcggact cgatgcgcgc
      481 tgggcaatcc gccccgatct cgaatggtcg gcgacgctca atccggactt ctcgcaggtt
      541 gccccggacg tgctgcaatc gacggtgaac gaacgcttcg cgctcttcta tccggaaaac
      601 cgcccgttct tcgaggaagg aacctgggtg ttcaacacgc ccggtttcaa ttacggcgag
      661 caggacggca acaatcagtt cgtggacacg cgccagatcg ccgacccgca ctgggcctcg
      721 aaggtggtcg gccagatcgg cagcaacgcg ctcggagccc tggtcgccga cgattcgatc
      781 accaacattc tgatgccggg accggtgcag tcgaacctgc aatcattcga cttcggtacg
      841 cgcgacgcgc tgctgcgcta tcgccaggac ttccccggca actcctcgct cggagtactc
      901 gcgaccgatc gcgaaggcgg cggctacagc aacggtctcg tcggcctcga tggcagttgg
      961 cagctcgatc cgagcgactc gatcaccgcg cagctcgcgt acacggacac gaactatccc
     1021 gacgaagtcg ccttcgcctt cggcgtcgcg cccggacgcg tcaccggcaa cggctggctc
     1081 gtcagctttc agcgcacccg ccacaactac acgacgagtc ttctcctcgc ccgcgtcgcg
     1141 ccgggatttc gcgccgatct cggttatctc ccgcaggtcg gctacagcga ggcgatgccg
     1201 cagtacgaat acgactggta ttcaagcgac tcgtggtgga acaacggcgg cttcggcggc
     1261 agctacgact tcgtgcaggc gacgggcgac gggccggtgc tcgaccgccg ggcgcaggtc
     1321 tatgccttcg cgcacgcgat cggccagagc cacctcatct tcttcgcccg tcacgaagat
     1381 cagtatttcg gcggcgagac cttcgcgctc aatcagctcg agatggacgc aagcgcgcag
     1441 ccgctctcct ggctcgaggc ggaactcgac gtgaccggcg gcgacgacgt ggactacatc
     1501 ggggtgcgcg aaggcgggct cttgtccgtc tcgccctcgt tcacgctcac tcccggacgg
     1561 catctgcaaa ttcagttcgt cggcaacttc gagcgcctca acgtcaccgg cgggcggctt
     1621 tataccgcga atctttatga tctgcgcgtc gcctggtatt tcaattcgca gatgttcgtg
     1681 cgtgcgatcg cgcaggagca ggacgtgcgc aacaacacgg cgctctatcc gccgggaact
     1741 ccgagccgcg agcgtgcgct cgcgacccag tggctgttcg gctacgaact caatccctgg
     1801 acctcgctct tcgcgggatt caccaacaac tatctcgcga ccggagacgt cgggctgctg
     1861 cagcagggtc gaacctactt cgtcaagatg agctacgact tccagctctg agcggcggca
     1921 gcttccgcgt tcgcgtgttt tgccgctcat tccagccacc gcggatcgaa ggaacgcggc
     1981 acgggaatca tgcttgagtg atgcaactct cgcagaaaat ttcagcaaaa tttccttttt
     2041 tgttgactca ctctactcat cgtgttatgg tgcgaaagtg ctccaaggcg cttaat
//
LOCUS       DASUJG010000242         2350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1416301, whole genome shotgun sequence.
ACCESSION   DASUJG010000242 DASUJG010000000
VERSION     DASUJG010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416301"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..1338
                     /locus_tag="VFX38_05205"
     CDS             163..1338
                     /locus_tag="VFX38_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5314286.1"
                     /translation="MSRKPVYLDYAATTPVDPAVIAAMLPCLGPEGCFANPASVTHAP
                     GREARKAVEAARGEIAALVGAASEEIIFTSGATESDNLALKGVMRSRGGACHLVTVRT
                     EHKAVLDTAHRLEGEGFHVTYLTPQVDGRIRPEALAAAIREDTALVSIMWVNNEIGVM
                     QDIAALGAVCRERGLLFHVDAAQGAGKLPLDLGSLPVDLMSFSGHKLYGPKGIGALYV
                     RRAPHVRLEAMVHGGGHEGGLRSGTLATHQIVGMGRAFAIARERLADDAEHARRLSRR
                     LHEGLAALGGVEVNGAQDEWRAPPILNLAFDGIVSDSLLYFLTDVAVSTGSACSSASR
                     APSYVLKALGLSDARARSSLRLSVGRFTTVEDVDFAIAHIGDAVRRLRAIAPASAAG"
     gene            1335..1712
                     /locus_tag="VFX38_05210"
     CDS             1335..1712
                     /locus_tag="VFX38_05210"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEX5314287.1"
                     /translation="MIAAAESFGPLAREHLARPRNHGPFAPDAPGSVRSGEAGSRSAG
                     AFVRFHLRIEDGCIVAARYEVLGAPALIAACSYLSELLQGAPASPQAMPRGLDLARAL
                     ALPRAEHGAALLAEDAARAALTA"
     gene            complement(1853..2257)
                     /locus_tag="VFX38_05215"
     CDS             complement(1853..2257)
                     /locus_tag="VFX38_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HEX5314288.1"
                     /translation="MNAETAGSGAADPRIFFAAERTLLAWLRTGITLMAFGFVIARFG
                     LFLDLLAHQNIAATPREYPRVSAIIGVLLMLGGALAALGATLQHHRFVATLAPSQLPA
                     RYSLWFALFTGWLLALLGLVLAAYLAVTEFLV"
BASE COUNT          372 a          777 c          832 g          369 t
ORIGIN      
        1 gggtgatctc ggcccgcgcg ccgcgccata ccctgcgccg catgcagcgc ctcgcccgtc
       61 gggcgcgcct cgagcccggg gaggttaagc tgttgcgcgg atttctcggc gccgcggaag
      121 gcgccttggc ggcgtcgcgc ccgtcgcgcc cggaggatgg cgatgagtag gaagccggtt
      181 tatctcgact atgcggcgac gactccggtc gatccggcgg tgatcgcggc aatgctcccc
      241 tgtctcggcc ccgagggctg tttcgcgaac ccggcctccg tcacgcatgc gcccggacgc
      301 gaggcgcgca aggcggtgga agcggcgcgc ggcgaaatcg cggcactcgt cggcgcggct
      361 tcggaggaaa tcatcttcac ctccggcgcc accgaatcgg acaacctcgc gctcaagggc
      421 gtgatgcgca gccgcggcgg agcctgccat ctggtgacgg tgcgcaccga acacaaggcc
      481 gtgctcgata ccgcgcaccg cctcgaaggc gagggctttc acgttaccta tctcacgccg
      541 caggtggatg ggcgcatccg gcccgaggcg ctcgccgcgg cgatccgcga ggataccgcg
      601 ctcgtttcga tcatgtgggt caacaacgag atcggcgtaa tgcaggacat cgccgcgctc
      661 ggcgccgtct gccgcgagcg cggcctgctg tttcatgtcg acgctgcgca gggcgccgga
      721 aaactgccgc tcgatctcgg ttcgcttccg gtcgatctca tgtcgttttc cgggcacaaa
      781 ctctacggac ccaagggaat cggcgcgctc tacgtgcgac gcgctccgca cgtgcggctc
      841 gaagcgatgg tgcatggcgg cggccatgag ggcgggctgc gctccggaac cctggcgacg
      901 catcagatcg tcggcatggg gcgcgccttc gcaatcgccc gcgaacgtct cgcggacgac
      961 gccgaacatg cgcggcgtct cagccggcgg ctgcacgagg ggctggcggc gctcgggggc
     1021 gtggaagtca atggcgcgca ggatgaatgg cgcgccccgc cgattctcaa tctggccttc
     1081 gacgggattg tcagcgacag tcttctttat ttcctcaccg acgttgctgt ttccaccggt
     1141 tcggcatgca gttcggcttc gagggcgccg tcttacgtgc tcaaggcgct cgggctttcc
     1201 gacgcaaggg cgcgttccag cctgcgcctg agcgtcgggc gtttcacgac cgtggaagac
     1261 gtggacttcg cgatcgcgca catcggcgat gcggttcgcc gcctgcgcgc gatcgctccc
     1321 gcttcggccg cgggatgatc gccgccgcgg agagcttcgg tccgctcgcg cgggaacact
     1381 tggcgcggcc gcgcaatcac gggccgtttg cgccggacgc gcccggcagc gttcgcagcg
     1441 gcgaggcggg gagccgcagt gcgggagcgt tcgtccgctt ccatctgcgc atcgaagacg
     1501 gttgcatcgt cgcggcgcga tacgaagtgc tcggggcgcc cgcgctgatc gcggcctgtt
     1561 cgtatctctc ggagttgctg caaggcgcgc cggcttcgcc gcaggccatg ccgcgcgggc
     1621 tcgatctcgc ccgcgcactc gcgctgccgc gggccgaaca cggtgccgcg ctcctcgcgg
     1681 aggatgcggc ccgcgccgcg ctcacagcct agggaagctc tgaatattcc gtcataccga
     1741 cgcaggccgg tatccagtga aagaagaatc ccggcttata gccgggatac aagttcttac
     1801 tggaccccgg ctttcgccgg ggcgacgggc aacgcaattg atctgagctt ccctagacga
     1861 gaaactcggt aacggcgaga taggcggcga gaacaaggcc gagcagcgcg agcaaccagc
     1921 cggtgaaaag cgcgaaccaa agcgaatagc gcgccgggag ttgcgacggc gcgagcgtgg
     1981 caacgaagcg atgatgctgc agggttgcgc cgagcgccgc gagtgccccg ccgagcatca
     2041 ggagcacgcc gatgatggcg gagacgcggg gatattcccg gggagtcgcc gcgatgttct
     2101 gatgcgcaag aaggtcgaga aagagcccga atctcgcgat cacgaagccg aaggccatca
     2161 aggtgatgcc ggtgcgcaac caggcgagaa gcgtgcgttc cgcggcgaag aaaatgcgcg
     2221 ggtccgccgc gccgctgccc gcggtttcgg cgttcatcgg gtctgccgtg cgccgccccg
     2281 accgggacgg cccgcgaacg ggcggcgacg tcggattttg cgcaggcgat gccacgccgg
     2341 cgccgaggag
//
LOCUS       DASUJG010000243         2583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_317478, whole genome shotgun sequence.
ACCESSION   DASUJG010000243 DASUJG010000000
VERSION     DASUJG010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317478"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1732)
                     /locus_tag="VFX38_05220"
     CDS             complement(<1..1732)
                     /locus_tag="VFX38_05220"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5314289.1"
                     /translation="MDCGFLRLLPGYGDYLNGWSRRWSAGQALFLSVIVPALFVSAAW
                     AGGTKPIALASHTRLQQELAANQSRDYRLTLNAGQAVTLRLEQDQNRVALRQIAPANT
                     LPPRSSDAGRFAFVEWTLIAEQATTWIFRVQALAPDRPASYRIELDAAHPASADDRLR
                     ARTEAALSQAETQRRSHGAGKVKEVSGRYQTAIAGYRELHDACGEQAAQIGLARYQLD
                     RGQYADGQTAAEAALRLHCTPDARDLALRALALHTLSSARFYQGDFAGGVRFGKQSLA
                     LSRRTRDLQSQEGTLGNLVGDYAELGESDHALDMAHAARKLALQLGDRRNALMDQGNI
                     ASIHLSRGELMPALAGYRKSLALLQAAPDPYLEGLVWTNLGMLYNVLGDNAASLDAYA
                     RAIALWAEQQNQPELSAALWNQGFVFLRQEQNRQACAVFQRALKVTETANLENDRTHV
                     LEGLGRCAMAESDWPRARDYLEQSLTLAKKIGNPAAEIPGTLALGDLALARRDFAGAR
                     TQYAQAYKLALRYHDRDSQPSALAGLARADRDSGNLDAAQQNVGRALALIADQRRQIA
                     DPGLRTDYFAD"
     gene            2007..>2583
                     /locus_tag="VFX38_05225"
     CDS             2007..>2583
                     /locus_tag="VFX38_05225"
                     /inference="COORDINATES: protein motif:HMM:NF042610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6789 family protein"
                     /protein_id="HEX5314290.1"
                     /translation="MTRSISKGIAAGFFATLIMGLALFAAAGLNFYPPLIRRLALISH
                     DVIGSPMWLWIGWAYHFAIGTLAWGILFGLVACAVRARSRFGTWFTAIVFAACAWVLM
                     MVIVMPLSGAGFFARSVGLGPLIVMAIWHFIWGTVLGLSYYAMTKPAVIEHRVHDRII
                     GVPIFRGFRWRRSHQTEEPLPPTERPAPRAGE"
BASE COUNT          438 a          869 c          812 g          464 t
ORIGIN      
        1 ggtcggcgaa gtagtcggtg cgcaggcccg gatcggcgat ctgcctgcgc tggtctgcga
       61 tgagcgcgag agcgcgcccg acgttttgct gcgcggcatc gagattgccg gaatcccgat
      121 ccgcccgcgc caggccggcg agcgcggacg gctggctgtc gcgatcgtgg taacggagcg
      181 cgagcttgta ggcttgcgca tactgggtgc gcgccccggc gaagtcgcgc cgggcaagcg
      241 cgagatcgcc cagcgccagc gtccccggaa tctcggccgc gggattgccg atttttttgg
      301 ccagcgtcaa cgattgttcc agataatcgc gcgcccgcgg ccaatccgat tcggccatgg
      361 cgcagcgccc caggccttcg agcacgtgcg tacgatcgtt ctcgagattt gccgtttcgg
      421 tcaccttcaa ggcccgttga aacaccgcac aagcctgcct gttctgttcc tgccggagaa
      481 aaacaaaacc ctggttccag agcgcggccg acaattccgg ctggttctgc tgctccgccc
      541 agagggcgat tgccctggcg taggcatcga gcgaagccgc gttgtctccg agcacgttgt
      601 agagcatccc cagattggtc cacaccagac cctcgaggta gggatcgggt gcggcctgca
      661 gcagcgcgag acttttgcga tagccggcca gtgccggcat caattcgccg cgcgacaaat
      721 ggatggaagc gatgttgccc tgatccatca gcgcgttgcg ccgatcgccg agttgcagcg
      781 cgagcttgcg cgcggcgtgg gccatgtcca aagcgtggtc actttcaccc agttcggcat
      841 aatcgccgac cagattgccg agcgttccct cctgcgactg aagatcccgc gtgcgccggg
      901 agagcgcgag cgattgtttt ccgaagcgaa cgccgccggc aaagtcccct tgatagaagc
      961 gcgccgaaga caaggtgtgc aaggccaggg cacggagcgc caagtccctt gcgtcgggcg
     1021 tgcaatgcag gcgcaacgcc gcctcggccg ccgtttgccc atcggcatat tgcccgcggt
     1081 cgagttggta ccgcgcaagt ccgatctgcg cggcctgctc gccgcaagcg tcgtgcagtt
     1141 cgcgatagcc ggcaatggcc gtttgatacc ggcccgacac ttccttcact ttccccgcac
     1201 catgactgcg ccgttgcgtc tccgcctgcg aaagcgcagc ttccgtgcgc gcccgcaaac
     1261 gatcgtcggc gcttgccgga tgcgcagcat ccaattcgat ccggtagctc gcggggcgat
     1321 ccggcgcgag cgcctgcacc cgaaagatcc aggtcgttgc ctgctccgcg atcagggtcc
     1381 attccacgaa ggcgaaacgg ccggcgtcgc tggaacgggg cggcagggta ttcgccggag
     1441 cgatctgccg caacgcgacg cgattttgat cctgctccaa ccgcagagtt acggcctgtc
     1501 ccgcgttcag cgtcaggcga tagtcgcggc tctgattcgc ggccagctcc tgctgcagcc
     1561 gagtatgcga ggcgagagcg atgggcttcg taccgcccgc ccaggcggcg gaaacgaaca
     1621 gcgcgggaac gatcaccgac aggaagaggg cctggccggc tgaccaacgt ctcgaccaac
     1681 cgttcaggta atcaccgtac ccgggcagca aacgcaggaa cccgcaatcc acgacccgtt
     1741 ccctcccaag gcgttttgct tatcttagct ccgccgcgag ccggaagcgt tccgcgcaag
     1801 accggcgaaa cgaatctgca atcacggctg ccaaacctga ggccgttcgc gtgtcgccgg
     1861 ctccgactgc cacgatcgcg actgacttcg aacgggacgg cgttcgcccc ctgaaaacaa
     1921 gacgctcgac ggagtagagt ggaattgtca actccaccca aacaggcggt gccggcaggc
     1981 ccgccgtcga acaaggagac atttccatga cgcgcagcat ctccaagggc atcgcagccg
     2041 gcttcttcgc caccctcatc atgggcctgg ccctgttcgc cgccgccggg ctcaacttct
     2101 atccgccgct catccgcagg ctcgcgctga tttctcatga cgtgatcggc tcgccgatgt
     2161 ggctgtggat cggctgggcc tatcacttcg cgatcggaac actcgcctgg ggcatcctgt
     2221 tcggcctcgt cgcctgtgcc gtacgcgcgc gcagccgttt cggaacctgg ttcaccgcca
     2281 tcgtctttgc ggcctgcgcc tgggtgttga tgatggtgat cgtgatgccg ctttccggcg
     2341 ccggcttctt tgcccgcagc gtcgggctcg gcccgctgat cgtgatggcc atctggcact
     2401 tcatctgggg gacggtactc gggctcagct actacgccat gaccaagccg gcggtgatcg
     2461 agcatcgcgt gcatgatcgc atcatcggcg tgccgatttt ccgcggattc cggtggcgcc
     2521 gttcgcatca aaccgaagag ccgctgcccc cgaccgaacg gccggcgccc cgcgccggcg
     2581 aac
//
LOCUS       DASUJG010000244         8397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1173211, whole genome shotgun sequence.
ACCESSION   DASUJG010000244 DASUJG010000000
VERSION     DASUJG010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8397
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173211"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1162
                     /locus_tag="VFX38_05230"
     CDS             <1..1162
                     /locus_tag="VFX38_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HEX5314291.1"
                     /translation="ELVTLGEVLRSEAYERAHSKLTVALGRDIGGQPVVTDLGRMPHL
                     LIAGTTGSGKSVALNAMILSLLYKATPDEVRLLLVDPKMLELSVYEDIPHLLAPVVTD
                     MSEATNALRWCCVEMDRRYRRLAALGVRNLAGFNRKLQELAEKGETVPDPTIEADEDG
                     VRPALEPLPYLVVVIDELADLMLVVGKKAEELITRIAQKARAAGIHLVVATQRPSVDV
                     ITGLIKANIPARVAFQVSARVDSRTIIDQGGAEALLGSGDMLFLPPATSLVQRVHGAF
                     VDDAEVHRVVEWLRKSGKPAYVEEVLAANEEGSAAASGIAAAGSEDDPLYDEAVRIVT
                     ETRRASVSSVQRRLRIGYNRAARLVEEMERVGIVGPLQSNGNREVLAGPPPE"
     gene            1263..1913
                     /gene="lolA"
                     /locus_tag="VFX38_05235"
     CDS             1263..1913
                     /gene="lolA"
                     /locus_tag="VFX38_05235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEX5314292.1"
                     /translation="MKVLFLLVASLVSLTALGAATTPAQRLDAILARTKTLSADFTET
                     VTNANAAMVKQSSGSVAVARPGKFRWDYRKPYRQIIVADGENLWIYDPALEQVTVRPE
                     SRALTAGPAALLAGSGDIATSFVVSDLGDSGGFDWLKLVPRSGDANYRAIRIALAANG
                     EIRALELDSKLGETSRINFSHVRSNISMDASRFRFTPPAGADVVHEAPNSASSAGP"
     gene            2108..3436
                     /locus_tag="VFX38_05240"
     CDS             2108..3436
                     /locus_tag="VFX38_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX5314293.1"
                     /translation="MSASTPHQPASVPLAERMRPQRLEEVVGQDALIGPGGTLARALA
                     GGLLPSLILWGPPGSGKTTLARLLAEARGARFFALSAVNAGVRDVKDVVAAAEQLRAE
                     SRDAVLFLDEIHRFNKAQQDALLPHVERGTVILIGATTENPSFELRGALLSRTRVVKL
                     APLDEAALAVILERALTDRERGLGLSVDVIPSEWRADIADAASGDARRALNLLEAVAA
                     AHGNEALDPSGFHAVLGERLALFDKGGDLFYDQISALHKSIRGSAPDAALYWFARMLE
                     GGADPLYIARRLARMATEDIGLADPRALSFALDAWGAYERLGSPEGELALAEVVVYLA
                     VAPKSNALYKASAAAREDAKEFGAAAVPIHLRNAPTRLMKEMGHGAAYRYAHDEPEAY
                     AAGERYFPDGMPDRRYYRPSDRGIEARISERLRHLGELDAKARNDRKLER"
     gene            3610..4650
                     /locus_tag="VFX38_05245"
     CDS             3610..4650
                     /locus_tag="VFX38_05245"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5314294.1"
                     /translation="MTTTQSGATESRPLEILGIGAAEESIYDWLLTHAGATVHEIARA
                     RAVTAGKVQRLLDAMASKGLVTHAPERPRRYIAAAPDIALKALALRRQEGLRRAEAEI
                     QKLQRRAAAAQRRGEQEQIVELVTTRAAEAQILEQVQRAAQHEFVSLMRSPMRVSRLE
                     DSSETDSRTQREAQARGVRYRSIVDAQYLELPGAVRRMRSDAEAGEEVRIFPGLPLKL
                     VLADRRIGIIPLKLQDADGPSLIVRSSALLDAIYALFEMLWERAAPVSFGAFGAIEAG
                     DAAALFSSEAEKLIDLMTTGLNDKRLADELDISMRTLRRRTVELMKSLNAHTRFQAGW
                     IAASRRYGHKPL"
     gene            4778..7270
                     /locus_tag="VFX38_05250"
     CDS             4778..7270
                     /locus_tag="VFX38_05250"
                     /inference="COORDINATES: protein motif:HMM:NF025670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314295.1"
                     /translation="MNRTVRQALASLSIPLAFAAGPTFAATSVSPAGPALDAAHVRSL
                     PPALRPVLYAALSGDAQAAYRFDADGCASLPGRGLGACFDRGGAHFSGGDSSLALRLI
                     AYGRGGADPSPAKLIHVTPVSPTFHRNRVNYRHAALTEWWRVLPVGFEQGFTLSRRPS
                     GNGHLQLILATNDRAERRDGILSWGRLNYGGLVVVDAAGHTVPATLRSGGNRILIAVN
                     DAHAAYPLTIDPLLWIEQKALASDGAAFNAFGNAVAVAGSNALVGAPDVNGWEGAVYA
                     FAYENGIWTQTQKFFADDGVSGDGANFGATIAMDGSTALIGAVGATVNGNSAQGAAYV
                     FTESNGAWSQEAKLVSSDGTANNYFGQGVSIDGDTALVGAYGASVHGNSLQGEAYVFT
                     NSGGTWTQAQILTAADGAASAFFGHSVGLSGTTAVVGAPYAAVGGNTVGAAYLFTDSG
                     GSWSQTQKLIANDGTGGAGFGYSVALSGTTALIGANGQNVGSNSFQGAAYIFSDSGGT
                     WTQAAELTSNDGVGGDDFGYAVALANSGVTALIGADRRTVNGNLNQGAAYRFTDAGGS
                     WTQTAEYVAGDGATGDFYGAAVAVSDDTSLVAAPIKTVKGNAVQGEAYFYDGGDLGLA
                     VSAPQTVGQGQQYVSQTIATNNASAASPAVSATIAVPADASFVAATATQGSCSETSGL
                     VTCDFGAIGGNAGTATANVTLKATGNVGDTIENVSGIAKATPPLTASAATTITEGGAC
                     PGGYSEYDGDLAAEERAHSPIYRAPAGVENAILDGPSGFQLYGVFQNARGRKTYRIPG
                     NEVHRSAPAGTFTWVVKAGDAGGAYTLCVLHP"
     gene            7639..>8397
                     /locus_tag="VFX38_05255"
     CDS             7639..>8397
                     /locus_tag="VFX38_05255"
                     /inference="COORDINATES: protein motif:HMM:NF025670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HEX5314296.1"
                     /translation="MLAKDAGAAYDVDGRGCATLPKSGLAACFDDHGVRFSGADVLLA
                     LRLVGFGRSGDVTPVAPVRPAIAGNRVRYVHGALSEWWRVLPTGFEQGFTIAKRPGGR
                     GWLLLTLSTTRQSRESGNPVRTVGTSLSIPLRPEAGKEIASGKLRYGGLVVADAKGKI
                     IPATLRSAGDRIRIAVNDAGAAYPLTVDPMVWLEQKATASDGAVGDSLGYSVAISGTI
                     ALVGAPGATVNGNASQGAAYVFTEAGGSWIQSAKL"
BASE COUNT         1467 a         2640 c         2775 g         1515 t
ORIGIN      
        1 cgaactcgtg accctcggcg aggtacttcg ctcggaggcc tacgagcgcg cccactcgaa
       61 gctcacggtc gcactcggcc gcgacatcgg cggccaaccg gtcgtgaccg atctcgggcg
      121 catgccgcat ctgcttatcg ccggcactac cggctccgga aagtcggtcg cgctcaatgc
      181 gatgatcctg agtctgctct acaaggcgac gccggacgaa gtgcgcctcc tcctcgtcga
      241 cccgaagatg ctggaactgt cggtgtacga ggacattccg catcttctcg ctccggtggt
      301 gacggacatg agcgaggcga ccaatgcgct gcgctggtgc tgcgtcgaga tggatcggcg
      361 ctatcgccgc ctcgccgcgc tcggagtgcg caacctcgcg ggcttcaacc gcaagctgca
      421 ggaactcgcc gagaagggcg agaccgttcc cgatccaacc atcgaggcgg atgaggacgg
      481 cgtgcggcct gcgctcgaac cgcttcccta tctggtcgtc gtgatcgacg aactcgcgga
      541 cctcatgctg gtggtgggga agaaagcgga ggaactcatc acccgcatcg cgcagaaggc
      601 gcgcgccgcc ggcattcatc tggtggttgc gacgcagcgt ccctccgtgg acgtgatcac
      661 ggggctcatc aaggcgaaca ttccggcacg cgtcgcgttt caggtgtcgg cgcgggtgga
      721 ttcgcgcacc atcatcgatc agggcggtgc cgaggcgctt ctcggcagcg gcgacatgct
      781 gtttcttccg ccggcgactt ccctcgtgca acgtgtgcac ggcgctttcg tcgacgatgc
      841 cgaggttcat cgggtggtcg agtggctgcg caaatcgggg aaacccgcct acgtcgagga
      901 agtgcttgcg gccaacgaag aggggagcgc cgccgcaagc ggcatcgccg cagctggcag
      961 cgaggacgat ccgctgtacg acgaggcggt gcgaatcgtt accgaaaccc ggcgcgcctc
     1021 ggtgtcctcg gtgcagcggc gtcttcgcat cggctacaac cgtgcggcgc ggctcgtcga
     1081 ggaaatggaa cgggtcggga tcgtcgggcc gctgcaatcg aacggcaatc gcgaggtgct
     1141 cgcgggtccg cccccggaat gaaatcgcgt tctggttgtc gaaagatccg caatcacacc
     1201 ctcatccgct tcgtcgaggt tggaacgcgg cccggcgccg gcagtttcac gaaggaaatt
     1261 tcatgaaagt cttgttcctg ctcgttgcat ccctggtatc cctgactgcg ctgggcgccg
     1321 cgaccacgcc ggcgcagcgt ctcgatgcga ttctcgcgcg cacgaaaacc ctgagcgcag
     1381 acttcaccga gacggtaacc aacgcgaatg ccgcgatggt caagcagtcc tcgggctccg
     1441 tcgccgtcgc gaggcccggg aaattccgct gggattaccg caaaccgtac cgccagatca
     1501 tcgttgccga cggcgagaat ctctggatct acgatccggc actcgagcag gtaaccgtgc
     1561 ggcccgagtc gcgggctctc acggccggcc ccgcggcact tctcgccggc tcgggcgaca
     1621 ttgccacgag tttcgtcgtt agcgatctcg gagactccgg cggtttcgac tggttgaaac
     1681 tcgtgccccg gtcgggcgat gcaaattacc gcgcgatccg catcgccctt gcggcgaacg
     1741 gggagattcg cgcgctcgaa ctcgattcga agctcggcga gacatcgcgc atcaatttct
     1801 cccatgtgcg tagcaatatt tcgatggacg cctcgcgctt ccggttcacg ccaccagcgg
     1861 gcgccgacgt cgtccatgaa gctccgaatt ccgcaagctc cgcaggtcca tgaccataat
     1921 cacaggcagg agcgagcttg ctcgcgaact tgcgacgatc ggcatacaga atcgcgagct
     1981 ttcgctcgct cctacaacag cgtttatcgg ctggcccatc agcttgcgat ttcgctgtga
     2041 caggtaggag tgagcttgct cgcgatcttg cgctgatcgc tttccggcat cacgcgccca
     2101 gaacaggatg agcgcatcca ccccgcacca acccgcgagc gtgccgctgg ccgagcggat
     2161 gcgcccgcaa cggctcgaag aagtcgtcgg tcaggacgcg ctgatcggac ccggcggaac
     2221 gctcgcgcgc gcgcttgccg gcggattgct cccttcgctg attctctggg gaccgccggg
     2281 gagcggcaag acgacgctcg cgcgccttct cgccgaagct cgcggcgcgc gtttctttgc
     2341 gctctccgcc gtcaatgccg gcgtccggga cgtcaaggat gtcgtcgcgg ccgcggaaca
     2401 gttgcgcgcc gagagccgcg acgcggtgtt gtttctcgac gagattcacc gcttcaacaa
     2461 ggcgcagcag gatgcgctgc tcccgcatgt ggaacgcgga accgtcatcc tcatcggcgc
     2521 gacgaccgaa aatccgtcat tcgagctgcg cggtgcgctg ctgtcgcgaa cgcgcgtggt
     2581 aaaactcgcg ccgctcgatg aggcggcgct cgccgtcatt ctcgagcgcg ccttgacaga
     2641 tcgcgaacgc ggtctgggat tgtcggtgga tgtcatacca agcgagtggc gcgccgatat
     2701 cgccgacgcc gccagcggtg acgcgcgccg cgccctcaat ctcctggagg cggttgccgc
     2761 cgctcacggg aacgaagcgc tggatccgag cggctttcat gccgtacttg gcgagcgtct
     2821 cgcgcttttc gacaagggcg gcgatctttt ctacgatcag atttcggcgt tgcacaaatc
     2881 catccgcggc tcggcccccg atgccgcgct ctactggttt gcgcgcatgc tcgaaggggg
     2941 cgccgatccg ctttatattg cgcggcgcct cgcacgcatg gcgacggaag acatcggtct
     3001 cgcggatccg cgcgcgctat cgtttgcgct tgacgcctgg ggtgcctacg aacggctagg
     3061 cagccccgag ggcgaactcg cactcgccga ggtcgtcgtc tatcttgccg tcgccccgaa
     3121 aagcaacgcc ctgtacaagg cgtccgcggc cgctcgcgag gacgcgaagg aattcggcgc
     3181 cgcggctgtg ccgatccatc ttcgcaatgc gccgacgcgc ctcatgaagg aaatgggtca
     3241 cggcgcggcg taccgctacg cgcacgacga gccggaagcc tacgccgcgg gcgagcgcta
     3301 cttccccgac ggcatgccgg atcgacgcta ttaccgtccg agcgaccgcg gcatcgaagc
     3361 gcgcatctcg gaacgcctgc gccatctcgg agaactcgac gccaaggctc gcaacgaccg
     3421 caagctcgaa cgctgacagg ctgcgaaaac cggtgatgtc cgctggaatt cgtggctgtg
     3481 caggagcgag cgtcagcgcg cgatggtcga cgaacgaaat tgcgggcaag ctcgctcttg
     3541 cgaaagagtc tggcgtagtg gttggaagca ggaacttttg gccacccgtc aaggaatgag
     3601 gagtccgtca tgactacaac acaatccggc gccaccgaat ccagaccact cgaaattctc
     3661 gggatcggcg cagcggaaga gtcgatctac gactggctgc tgactcatgc gggcgcgacg
     3721 gtgcacgaga ttgcgcgggc gcgggccgtg acggccggga aggtccagcg cctgctcgat
     3781 gcgatggcct ccaagggcct tgtcacgcat gcgcccgaaa ggccgcgccg ttacatagcc
     3841 gccgcgccgg atatcgcact gaaggctctg gcgcttcgcc gccaggaagg cttgcggcgg
     3901 gcggaggccg aaatccagaa actgcagagg cgtgccgcgg cggcacaacg gcgtggcgag
     3961 caggaacaaa tagtcgaatt ggtgaccact cgcgccgccg aggcgcagat tctggagcag
     4021 gtacagcgcg cggcacagca tgaattcgtt tcgctcatgc gctcgcccat gcgagtttcc
     4081 cgcctcgaag attcctccga aacggattcg cggacccaga gggaagcgca ggcgcggggc
     4141 gtgcggtatc gaagcatcgt cgatgcgcag tatctcgagc ttcccggagc ggtgaggcgg
     4201 atgcgcagcg acgccgaggc cggcgaggag gtgcggatat ttccggggct tcccttgaaa
     4261 ctggttctcg ccgatcggcg catcgggatc atccctctca agctccaaga cgcggacggc
     4321 ccctcgctca tcgtgcgttc ttccgcgctg ctcgatgcca tttatgcgct cttcgagatg
     4381 ctctgggagc gcgccgcccc cgtttctttc ggcgccttcg gggcgatcga ggccggcgac
     4441 gccgccgcgc ttttttcgtc ggaagccgaa aagctgattg atctgatgac gacgggactc
     4501 aatgacaaga gacttgccga cgaactcgat atttccatgc gcaccctgcg acgccgcacc
     4561 gtcgagttga tgaaaagcct caacgcacac acgcggtttc aggccggctg gatcgccgct
     4621 tcgagacggt acggtcacaa accgctttga accccgccga gctgtggcct aacagtgcca
     4681 cggcacggtt tggacagaaa ttccgttgat tttgtgtcgc cgccgggcgg atactggggc
     4741 cgctgatcgc acaattcttc ccgaaaggag acaaacgatg aaccggaccg ttcggcaagc
     4801 cttggcttca ctttccattc cgctcgcgtt cgccgcgggc ccgacgtttg cggcgacgag
     4861 cgtatccccc gcagggccgg ctctcgacgc tgcgcatgtc cgctcccttc cgccggcatt
     4921 gcgcccggtg ctgtacgccg cgctctccgg cgatgcgcaa gcagcctacc gtttcgacgc
     4981 cgatggttgc gcgagcttgc ccggccgcgg actcggcgcg tgtttcgatc gcggcggcgc
     5041 gcatttttcg ggcggcgatt cgtcgcttgc gctgcgtctg atcgcctacg gtcgcggcgg
     5101 agccgatccc tctccagcga aactgattca cgtaactccg gtgtcgccga cctttcaccg
     5161 gaatcgtgtg aactatcgcc acgctgccct gacggaatgg tggcgcgttc tgccggtggg
     5221 attcgaacag ggcttcacgc tgtcccggcg gccatccggc aatgggcacc tgcaactgat
     5281 ccttgcgacc aatgatcgcg cggagcgccg cgatggcatt ctctcctggg gccggttgaa
     5341 ctacggcgga ctcgtcgtgg tcgatgccgc gggtcacacc gtgccggcga cgcttcgaag
     5401 cggcggaaat cggattctga tcgccgtgaa cgatgcccac gccgcctatc cgctcacgat
     5461 cgatcccctg ctgtggatcg aacagaaggc ccttgcgagc gatggcgcgg ccttcaacgc
     5521 attcggcaat gcggtggccg tcgctggttc caacgcgctt gtcggtgcgc cggatgtgaa
     5581 cggttgggaa ggtgcggtct atgcatttgc ctatgagaac ggcatctgga cgcagacgca
     5641 gaagtttttc gcggacgatg gcgtgtccgg cgacggggcc aatttcggtg ccacgatcgc
     5701 gatggatggt tcgaccgctc tcatcggcgc ggtgggtgcc acggtcaacg gaaacagcgc
     5761 tcagggcgcc gcgtacgtgt tcaccgaatc gaacggcgcg tggtcgcagg aagccaaact
     5821 tgtgtcgagc gacggcaccg cgaacaatta tttcggccag ggggtttcca ttgacggaga
     5881 caccgctctg gtcggagcat acggcgcgag cgtacatggc aacagtctgc agggagaagc
     5941 ctacgtattc acgaattccg ggggaacctg gacgcaggcc cagattctga ccgcggccga
     6001 cggagcggcg agcgctttct tcgggcactc ggtcgggctt tcgggtacga ccgccgtggt
     6061 cggcgcgcct tatgcggcgg tcggcggcaa cacggtgggc gcggcctacc tgtttaccga
     6121 ttccggcggg agctggagtc agacgcaaaa gctcatcgcg aacgatggca ccggcggcgc
     6181 aggtttcggt tactccgtgg cgctttccgg cacgaccgct ctgattggcg cgaacggtca
     6241 aaacgtcggc agcaattcct ttcagggcgc ggcctacatc ttcagcgatt cgggcggaac
     6301 ctggacgcag gcggcggaat tgacgtcaaa cgatggcgtc gggggtgacg acttcggcta
     6361 tgccgtggct cttgcgaatt cgggcgtcac cgcattgatc ggagccgatc gccgtacggt
     6421 gaatggaaac ctgaatcagg gtgcggctta tcggttcacc gacgcgggag gttcctggac
     6481 acaaaccgcc gaatacgtcg ctggcgatgg agctaccgga gatttctacg gcgccgcggt
     6541 ggcggtcagc gatgacacct cgctcgtcgc cgcacccatc aaaactgtca aaggcaatgc
     6601 cgtgcagggt gaggcgtact tctacgatgg cggcgatctc ggtttggccg taagtgcgcc
     6661 gcaaacggtg gggcagggcc agcagtacgt cagtcagacg attgcgacca acaatgcaag
     6721 tgcggcttcc cccgcggtga gcgcgacgat tgcggtgccc gctgacgcga gttttgtcgc
     6781 tgccacagcg acgcagggca gttgcagcga gacttccggg ctcgtgacct gcgatttcgg
     6841 tgcgatcggc ggaaacgccg gcacggcgac ggcgaacgtc accctgaaag cgaccggcaa
     6901 cgtcggcgac acgatcgaga atgtgtcggg cattgccaaa gccacgccgc cgctcaccgc
     6961 gagcgccgcg acgacgatta ccgaaggcgg cgcctgcccg ggcggctaca gcgaatacga
     7021 tggcgatctg gccgccgaag agcgggcgca ctccccgatt taccgcgcac ccgcgggagt
     7081 cgagaacgcg attctcgacg gcccgtcggg ctttcagctt tacggcgtgt tccagaatgc
     7141 gcgggggcgg aagacctacc gcattccggg caacgaggtg catcgcagcg caccggccgg
     7201 taccttcacc tgggtggtga aggcgggcga cgccggcggc gcctacacgc tctgcgtgct
     7261 gcatccttga tgcaggggca ggatgccgtg cgtcttcgaa ccatatcgtc ccggttcgga
     7321 cggcgatatg agttgcaagg gcagggcatt ctgcgattcg ctacaggcag gagcgagcac
     7381 gaggtcgcga gcgagcgtgt tcatggcgga agacaaggcc gggcagcggc aagcaaatca
     7441 cggcggcgtt tctcgttcat tttcaggagt caaacgatgc aatggtccgt tgtccgaagg
     7501 ctggtcttca tgcttgcgtg tgggagcgct tggggcgtgg cactggccgg ctcgccggga
     7561 tccgtgacgg gggcagctcc cgaagcgccg tttcattccc cgactctgcc gccgtcgttg
     7621 cgtccggcgt tgtatcgggt gttggcgaaa gatgccggcg cggcctacga cgtggatggg
     7681 cgcggttgcg cgacactgcc gaagtccgga cttgcggctt gcttcgacga tcatggcgtg
     7741 cgcttttcgg gagcggatgt gctcctggcg ttgcggctcg tcggttttgg acgcagcgga
     7801 gacgtcacgc cggttgctcc ggtgcgcccc gcgattgcgg gtaaccgcgt gcgctacgtt
     7861 cacggtgcac tcagcgaatg gtggcgggtg ttgccgacgg gtttcgagca gggcttcacg
     7921 attgcgaaac gcccgggcgg tcgcggttgg ctcctgctca cgctttctac gactcgtcaa
     7981 tcccgcgaaa gcggcaatcc agtgaggaca gtggggacat cgctctcgat cccccttcgt
     8041 cccgaagcgg gaaaagaaat tgcatcgggc aagctgcgtt acggcggtct cgtcgtcgcc
     8101 gatgcgaagg gcaagatcat tccggcgaca ctaagaagcg caggcgatcg catccggatc
     8161 gccgtgaacg atgccggcgc tgcctatccg ctcaccgtgg acccgatggt gtggctggaa
     8221 cagaaggcca cggcgagcga cggtgcggtc ggcgacagtc tcgggtattc ggtcgcgata
     8281 tcgggcacga ttgcactcgt gggcgcgccc ggtgcgaccg tcaacggcaa tgcctcccaa
     8341 ggcgcggcgt acgtgtttac cgaagctggt ggcagttgga tccaaagcgc caaattg
//
LOCUS       DASUJG010000245         2277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_439771, whole genome shotgun sequence.
ACCESSION   DASUJG010000245 DASUJG010000000
VERSION     DASUJG010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439771"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VFX38_05260"
     CDS             <1..509
                     /locus_tag="VFX38_05260"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HEX5314297.1"
                     /translation="VVIAILIVVGLWLWRSGIIHGSSANPPAAAVSAAMAMTSPLPMH
                     SGAARVAPPPLRPVEAGSGAAAAGASSRLELKLAVSSWVEVYDAHGRRLYYDLASAGQ
                     ILKLQSSALPFRIFLGNAAGASIELNGKPVSVPAAGTSGTGVRFEVPAATASAASPAS
                     VSAPGAAT"
     gene            506..1822
                     /gene="hisS"
                     /locus_tag="VFX38_05265"
     CDS             506..1822
                     /gene="hisS"
                     /locus_tag="VFX38_05265"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002707416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX5314298.1"
                     /translation="MSGALRAVRGFNDILPAQVAAWRAIENTAAEVLETAGYAEIRLP
                     LVEHTALFARSIGSETDIVAKEMYSFEDRGGEMLSLRPEATAGCARAGIAHGLFNGGQ
                     QRLWYGGAMFRHERPQKGRHRQFHQIGAEAFGIEGAGIEAELLALAARLWRRLGLRDY
                     RLEINSLGSPEDRARFRGALVEYFSDHAEDLDADERRRLATNPLRILDSKSETTRALL
                     AEAPDIGDYLGVEALAQREELAARLALLEIPYRINSRLVRGLDYYTGLVFEWTSEALG
                     AQNAFCSGGRYDHLVAELGGRPTPAAGWALGLERLVLLVEEAGRAAAPSLPDAYFIAL
                     GREAEHRALGLSERLRDALPSLRLVVDAGGGSLRAQLRRADKAGAALALIVGEAELSS
                     GTIAVKPLAGDDQQTLVAESRLAEICAERIAQRNPDQGNQSPGEQE"
     gene            1828..>2277
                     /locus_tag="VFX38_05270"
     CDS             1828..>2277
                     /locus_tag="VFX38_05270"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5314299.1"
                     /translation="MEGYSTEQETLTALRAWWDKNGKYVVGGLVIAALVIGGWRGWNY
                     WQSKRAGEAAGLYAAVVAAERQNNDPAIVKAAEALIAAYPDSAYGALAGLAEARAQVE
                     QQQLPAAETALRRVAGNSPDEGLAAVANLRLARVQIQAGDAKGALATL"
BASE COUNT          361 a          751 c          807 g          358 t
ORIGIN      
        1 ccgttgtgat cgccattctc atcgtcgtcg gcctgtggct ctggcgcagc ggcatcatcc
       61 atggcagcag cgccaatccg ccggccgccg ccgtgtcggc agccatggcg atgacgagcc
      121 cgctgccgat gcattccgga gctgctcgcg tggcaccgcc gcccctgcgg ccggtcgaag
      181 cgggcagcgg cgccgctgcg gccggcgcga gttcgcgact cgaactgaag ctcgccgtgt
      241 cgtcctgggt cgaagtgtac gatgcacacg gccgacgcct ttattacgat ctcgcgagcg
      301 ccgggcagat cctgaaactt cagtcttcgg cgctgccctt tcgaatcttt ctcggcaatg
      361 ccgcgggcgc atcgatcgag ttgaacggca agccggtgag cgttcccgcg gccggaacgt
      421 cgggaacggg agtccgcttc gaggttccgg cggcgacggc gagtgcggct tctccggcca
      481 gcgtttccgc gccgggcgcg gccacatgag cggcgcactg cgcgccgtgc gcgggttcaa
      541 cgacattctg cccgcgcagg tcgcggcatg gcgggcgatc gaaaacaccg cggccgaagt
      601 gctcgagacg gccggttacg cagaaatccg cctgccgctg gtcgagcaca ctgcgctctt
      661 tgcgcgctcg atcggctccg agaccgacat cgtcgcgaag gaaatgtaca gcttcgagga
      721 tcgcggcggt gaaatgctga gcctgcggcc ggaggcgacc gcgggttgcg cgcgcgccgg
      781 cattgcgcac ggactgttca acggcggaca acagcggctc tggtacggcg gcgcgatgtt
      841 ccgtcacgaa cgcccgcaga agggacgcca tcgccagttt caccagatcg gagccgaggc
      901 cttcggcatc gaaggcgcgg gcatcgaggc cgagctgctc gcgctcgcgg cgcgtctgtg
      961 gcggcgtctg gggctgcgcg attaccgtct cgagatcaat tcgctcggtt ccccggaaga
     1021 tcgtgcacgt tttcgcggcg cgctcgtgga atatttttcg gaccatgccg aagatctcga
     1081 cgccgacgag cgccgcaggc ttgcgaccaa tcccttgcgg attctggaca gcaagagcga
     1141 aacgacgcgc gcgctccttg ccgaggcgcc ggacatcggg gattatctcg gagtcgaggc
     1201 gcttgcgcag agggaggaac tcgccgcccg tctcgcgctg ctcgaaatcc cctaccggat
     1261 caattcgcgg ctggtgcgcg gcctcgatta ctacaccggc ctcgtgttcg agtggaccag
     1321 cgaggcgctc ggcgcgcaga acgcgttctg ctccgggggg cgctacgacc atctcgtcgc
     1381 ggaactcgga ggaagaccga cccccgcggc gggctgggcg ctcggtctcg aacggctcgt
     1441 cctgctggtg gaagaagcgg ggcgtgccgc ggcgccgagc ctgccggatg cctacttcat
     1501 cgcgctcggc cgggaggcgg aacatcgcgc actcggcctc tcggagcgtc tgcgtgatgc
     1561 gctgccgagc ctgcgtctcg tcgtcgatgc cggcggaggc tcgctgcgcg cccagttgcg
     1621 ccgggccgac aaggccggcg ccgcgctcgc gttgatcgtg ggcgaggcgg aactttcttc
     1681 gggtaccatt gcggtcaagc cgctcgccgg cgacgaccaa cagacgctcg tcgccgagtc
     1741 ccggctggcc gaaatctgcg ccgagcggat cgcgcaacgc aaccccgatc agggcaatca
     1801 atcgcctggc gaacaggagt aatcggattg gaaggatata gcacggaaca ggaaaccctc
     1861 acggcgctgc gcgcctggtg ggacaagaac ggcaagtacg tcgtcggcgg gctggtgatc
     1921 gccgccctcg tgatcggcgg ctggcgcggc tggaattact ggcagagcaa gcgcgcgggc
     1981 gaagcggccg gactgtatgc cgcggtcgtc gcggccgaaa ggcagaacaa cgacccggcg
     2041 atcgtgaagg ccgcggaggc gctgatcgcc gcctatccgg acagcgccta cggcgcgctt
     2101 gccggactcg cggaggctcg ggcgcaggtc gaacaacaac agttgccggc cgcggagacc
     2161 gctctgcggc gcgtcgcggg caattccccg gacgagggac tcgccgcggt ggcgaacctg
     2221 cgtctggcgc gggtgcagat tcaggcgggc gatgccaagg gcgcgctcgc aaccctt
//
LOCUS       DASUJG010000246         4906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1296439, whole genome shotgun sequence.
ACCESSION   DASUJG010000246 DASUJG010000000
VERSION     DASUJG010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296439"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1199)
                     /locus_tag="VFX38_05275"
     CDS             complement(<1..1199)
                     /locus_tag="VFX38_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX5314300.1"
                     /translation="MYRIVTLIIAAGAAAAIGAQARAAAPQSASAAASATPAAAAGTA
                     NPLLRPSPLPFQAPPFDKIKDGDFQPAIEEGMRQQLAEVEAIANNPAAPTFQNTIVAM
                     EKTGDLLNRAEMVFGLLTSANTDPTLQQVQQTEAPKLAAHNDAIFLNPKLWARVKTIY
                     GQRDKLKLDPESARLLWYYHEQFVHAGANLSEADKAKLKKLNEEAASLSSTFVNKLLA
                     ATKDGALVVSDKSELAGLSDGEIEAAAEAAKARGLKDKWVIPLQNTTQQPALNELTNR
                     ATRHELFENSWTRAEKGNADDTRATIAKLAKVRAEKAKLLGYPDFAAWKLTDQMAKTP
                     QTVDNFLSELVGPSTATARQEAEALQKLIDASGNTFTLEPWDWEYYAEKLRAKKYDFN
                     EDSVKPY"
     gene            1391..2437
                     /gene="tsaD"
                     /locus_tag="VFX38_05280"
     CDS             1391..2437
                     /gene="tsaD"
                     /locus_tag="VFX38_05280"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005220878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX5314301.1"
                     /translation="MRVLGIETSCDETAVAVHEAGRGIVREEVYSQTELHRLYGGVVP
                     ELAARDHLQRLAPMLRATLEALPPPDAIAYTAGPGLIGALLVGAALAQAFAAARGVPA
                     LAINHLEGHILAPLLDEPDLDFPFVTLLVSGGHTLLAEARGLGRYHRLGTTRDDAAGE
                     AFDKTASLLGLAYPGGPALARLAEGGRPGRFPLPRPMLDRPGCDFSFSGLKTAVMYAA
                     RSLPAGDEQGRADLARAFEDAVVETLSVKAERALAAKGLTTLVVAGGVAANTRLRAAL
                     GAMAAARGARAIFPPIGRCTDNAAMIAYAGWRRFAGGERGADGEVRARWPLETLGPPA
                     GPTDSGESHGSRAH"
     gene            2418..2777
                     /gene="folB"
                     /locus_tag="VFX38_05285"
     CDS             2418..2777
                     /gene="folB"
                     /locus_tag="VFX38_05285"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX5314302.1"
                     /translation="MDRVRIEQLEVETTIGIYEWERRIRQRVVFDLELATDVARAAAS
                     DRIEDALDYKAVAKRVRAVVESARFGLVESLAEAVAKLLRDEFAARWVRVAVRKPAAV
                     SGAAAVGVVIERGERDA"
     gene            2770..3258
                     /gene="folK"
                     /locus_tag="VFX38_05290"
     CDS             2770..3258
                     /gene="folK"
                     /locus_tag="VFX38_05290"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX5314303.1"
                     /translation="MPERDIYIAIGSNIEPARHIPAAVAELARFYAPLARSPAYRSRA
                     LGFDGPDFVNCVVRGRSNASAATVVARLKELERAHGRREDDAIGSRALDLDLILYGTE
                     VIAGPTLTVPRPDILEYAFVLRPLAELAPHLAHPQTGRDFAWHWRHFVGAGLPLAPES
                     LD"
     gene            complement(3316..3392)
                     /locus_tag="VFX38_05295"
     tRNA            complement(3316..3392)
                     /locus_tag="VFX38_05295"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3356..3358),aa:Pro,seq:cgg)
     gene            complement(3493..4461)
                     /locus_tag="VFX38_05300"
     CDS             complement(3493..4461)
                     /locus_tag="VFX38_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018146303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX5314304.1"
                     /translation="MKLETITALVGEDLRAVDRFIRERLASDIELINELARHIVASGG
                     KRLRPLVVLLAARAAGYEGERHIHLAAVVEFIHTATLLHDDVVDGSQRRRGQRTANAL
                     WGNAASVLVGDFLYSRAFQMMVEVQKLRVMAVLADATNAVAEGEVLQLMYCNDPDATE
                     ANYFAVIERKTARLFEAAARLGAVIAGRPREEEDAFGAYGLNLGIAYQLIDDVLDYGS
                     DAEAIGKNPGDDLAEGKPTLPLIRARDCSQGAQQDAIRRAIEQGGAGTFAAVRAAIMA
                     TDALAYTHGRAREFHQTAAAALAGLPASPHRDMLAALARFAIERDN"
     gene            4605..>4906
                     /gene="rplU"
                     /locus_tag="VFX38_05305"
     CDS             4605..>4906
                     /gene="rplU"
                     /locus_tag="VFX38_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011505782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX5314305.1"
                     /translation="MYAVILSGGKQYPVAPGEVVRLEKLAAAVGEAVEFDRVALVAAD
                     DGKLTVGSPLVSGCRVRGEVASQGRSEKIRVIKFRRRKNYRRLQGHRQSYTDVKIG"
BASE COUNT          736 a         1601 c         1693 g          876 t
ORIGIN      
        1 aaatacggtt tgaccgaatc ctcgttgaaa tcgtatttct tcgcgcgcag cttctccgcg
       61 taatactccc agtcccaggg ctcgagcgtg aaggtgttgc cgctcgcgtc gatcagcttc
      121 tgcagggcct ccgcttcctg acgcgcggtg gccgtcgagg ggccgacgag ttcggacaga
      181 aaattgtcga ccgtctgcgg cgtcttcgcc atctgatcgg tgagcttcca ggcggcgaag
      241 tcgggatagc cgagcaattt cgccttttcg gcccgtacct tcgcgagctt ggcgatcgtc
      301 gcgcgggtat cgtcggcgtt gcccttttcc gcccgcgtcc aggagttttc gaaaagctcg
      361 tggcgggtcg cgcggttcgt gagttcgttc agggccggtt gctgcgtggt gttctgcagc
      421 gggatcaccc acttgtcctt gagtccgcgg gccttcgccg cctcggcggc cgcctcgatc
      481 tcgccgtccg aaagcccggc gagttcgctc ttgtcggaga ccacgagcgc cccgtccttg
      541 gtcgcggcga ggagtttgtt gacgaaggtg gaactgagcg aggcggcctc ttcgttgagt
      601 ttcttcagct tcgccttgtc ggcctcggag agattcgcgc ccgcgtgcac gaattgctcg
      661 tggtagtacc agagcaggcg tgcggattcg ggatcgagct tcagtttgtc gcgctggccg
      721 taaatcgtct tcacccgcgc ccacagtttc gggttgagga aaatcgcgtc gttgtgcgcg
      781 gcgagttttg gtgcttcggt ctgctggacc tgctgcaggg tcggatcggt gttcgcgctg
      841 gtgagaaggc cgaaaaccat ctccgcgcga ttgagaagat cgccggtctt ctccatcgcg
      901 acgatcgtgt tctggaacgt cggcgccgcg ggattgttcg cgatcgcctc cacttcggca
      961 agctgttggc gcatgccttc ttcgatggcg ggctggaagt cgccgtcctt gatcttgtcg
     1021 aagggcggcg cctgaaaggg caacggactg ggtcggagca acggattggc ggttcccgcc
     1081 gctgcggcgg gggtcgcgct ggccgcggcg gaagccgact gaggcgcggc cgcgcgggcc
     1141 tgcgcgccga tggcggcggc ggcgccggcg gcaatgatca gagtgacgat gcgatacatg
     1201 gatgcaatct cctcgaaatt ccggatcgaa cgggggccac ggccggacca tcgcgagctt
     1261 cgcgccttcg gccgggaagc cgtcacttta ccctctgcgc cccctgcaag gcaaagcggt
     1321 ggcccacgtg cgaagcgcga cgcgggcatc gcccgcggtg tatcctcgcc ccgtgccgag
     1381 ggacggaacc atgcgcgtac tggggataga aacctcctgc gacgaaaccg cggtcgcggt
     1441 ccatgaagcc ggccgcggaa tcgttcgcga agaggtttac agtcagacgg agctgcaccg
     1501 gctctacggc ggcgtcgtgc cggagcttgc cgcccgcgat catttgcagc gcctggctcc
     1561 gatgctgcgg gcgacgctgg aagccctgcc tccgcccgac gccatcgcct acaccgcggg
     1621 accggggctc atcggcgccc tgctcgtcgg ggccgccttg gcgcaggcct ttgcggcagc
     1681 gcggggtgtg ccggcgctcg cgatcaacca tctagaagga catattctcg cgccgctgct
     1741 cgacgagccc gacctcgact tccccttcgt cacgctcctc gtctccggcg gccatacgtt
     1801 gctcgccgag gcccgggggc tcgggcgcta ccaccggctc ggcacgaccc gcgacgatgc
     1861 ggcgggcgag gccttcgaca agacggcgag ccttctcgga ctcgcctatc cgggtgggcc
     1921 ggccctggcg cgcctcgccg aaggcgggcg gcccggacga ttcccgctgc cccgcccgat
     1981 gcttgatcgt cctggctgcg acttctcttt cagcgggctg aagacggccg tgatgtatgc
     2041 ggcgcggagt ctcccggcgg gcgacgagca gggacgggcg gatctcgccc gcgccttcga
     2101 ggacgcagtg gtggaaacgc tcagcgtgaa ggcggaacgg gcgctcgcgg cgaaagggct
     2161 cacgaccctg gtcgtcgccg gcggcgttgc cgccaacacg cgactgcgag cggcactcgg
     2221 cgcgatggcc gcggcgcgcg gagcccgggc gattttcccg ccgatcgggc gctgcaccga
     2281 caatgcggcg atgattgcct acgccggctg gagaagattc gccggcgggg aacgcggcgc
     2341 ggacggcgaa gtgcgcgcgc gttggccgct cgaaactctc ggtccgcccg cggggccaac
     2401 cgattccgga gaaagccatg gatcgcgtgc gcattgagca gctcgaggtc gagacgacca
     2461 tcggcattta cgaatgggaa cgacgaatcc gccagcgcgt ggtgttcgat ctcgaattgg
     2521 ccaccgatgt cgcgcgcgcg gcggcgagcg atcgcattga ggacgctctc gactacaagg
     2581 cggtcgcgaa gcgggtgcgc gccgtggtcg aatcggcgcg attcggcctc gtcgaatcgc
     2641 tcgccgaggc cgtcgcgaaa cttctgcgcg atgagtttgc cgcccgctgg gtccgggtcg
     2701 ccgtgcgcaa gcccgcggcg gtgagcggcg ccgccgccgt gggagtcgtc atcgagcgcg
     2761 gcgagcgcga tgcctgaacg cgacatctac atcgcgatcg ggagcaatat cgagcctgcc
     2821 cgccacatcc cggcggcggt cgccgaactt gcgaggtttt atgccccgct cgcccgctcg
     2881 ccggcgtacc gatcccgggc gctcggcttc gacggcccgg atttcgtcaa ttgcgtggtg
     2941 cgcggccgca gcaacgcgtc ggccgccacc gtcgtcgcgc gcttgaagga gctggaacgc
     3001 gcgcacggac ggcgcgaaga tgatgcgatc ggctcgcggg cgctcgatct cgatctgatc
     3061 ctctacggaa ccgaggtcat cgccgggccg acgctcaccg ttccgcggcc cgatatcctc
     3121 gaatatgcct tcgtgttgcg gccgctcgcc gaactcgccc cgcatctcgc gcacccgcaa
     3181 acgggaaggg acttcgcctg gcattggcga catttcgtgg gcgcgggctt gccgctcgcg
     3241 ccggaatcgc tcgactgaac cccggtctgc gcgccgcgcg tccggcgccg gtcgtctcgc
     3301 aacggcatgg aaaaatggtc ggggtgacag gatttgaacc tgcgacccct acgtcccgaa
     3361 cgtagtgctc taccaggctg agctacaccc cgacctcgaa ccaccgcaat tctcgcgcgc
     3421 ccggaattct gcgcgcgggg gtcattttag caagatcgaa gccgcggcct gatgtgcagg
     3481 tggctctcgg gctcaattgt cgcgctcgat cgcgaagcgg gcgagagccg cgagcatgtc
     3541 tcgatgcggc gaagcgggaa gaccggcaag cgcggcggcc gccgtctgat ggaactcgcg
     3601 tgccctgccg tgcgtgtagg cgagcgcatc cgtcgccatg atcgccgcgc gcacggcggc
     3661 gaacgttccc gcaccgccct gctcgatggc gcggcggatg gcgtcctgtt gtgcgccctg
     3721 ggagcaatcg cgggcccgga tgagcggaag cgtgggcttg ccttcggcaa ggtcgtcgcc
     3781 cgggttcttg ccgatcgcct cggcatcgct gccgtagtcg aggacatcgt cgatgagttg
     3841 gtaggcgatc ccgaggttga ggccgtaggc accgaaggcg tcttcttctt cacgcgggcg
     3901 cccggcgatc acggctccca gcctcgccgc ggcttcgaaa agccgcgcgg ttttgcgttc
     3961 gatcactgcg aaatagttcg cctcggtcgc atcgggatcg ttgcagtaca tgagctgcag
     4021 gacctcgccc tcggcgacgg cgttggtggc gtcggcaagg acggccatca ctcgcagctt
     4081 ctgcacctcc accatcatct ggaatgcgcg cgagtagagg aaatcgccga ccagaacgct
     4141 cgccgcgttg ccccagagtg catttgccgt gcgctgtccg cgccgccgct gcgagccgtc
     4201 cacgacgtca tcgtgaagaa gcgtagccgt atggatgaat tcgacgaccg cggcgagatg
     4261 gatgtggcgt tcgccttcgt aacccgcggc gcgcgcggca agcagcacca ccagcgggcg
     4321 caggcgcttg cctccgctcg cgacgatgtg gcgggcgagt tcgttgatca gctctatgtc
     4381 ggaggcgagg cgctcgcgga tgaagcgatc caccgcgcgc aagtcctcgc cgacgagagc
     4441 ggtgatcgtc tccagtttca tcctgagccg ccccggggaa tgcgccgatc gtaggcagtt
     4501 gcgccgcccg aagtcaacgc agggcccccg cggcttgact cgttcgcgcc cgcgcgctta
     4561 tcatttcccg cttgctttcg gctcttttcg agacggtgcc ttgcatgtac gcggtcatcc
     4621 tttccggcgg aaaacagtat ccggtcgcgc cgggcgaagt ggtgcgcctg gagaaactcg
     4681 cggccgccgt cggcgaggcg gtcgaattcg accgcgtggc gctggttgcg gccgacgacg
     4741 gcaagcttac ggtaggcagt ccgctcgtga gcggctgccg ggtgcgcggc gaggtcgcga
     4801 gccaaggccg gagcgagaag atccgggtca tcaagtttcg ccgtcgcaag aattatcgcc
     4861 gtctgcaggg acatcgccag agttacacgg acgtcaagat cggcga
//
LOCUS       DASUJG010000247         2509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1174645, whole genome shotgun sequence.
ACCESSION   DASUJG010000247 DASUJG010000000
VERSION     DASUJG010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174645"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /gene="rpsC"
                     /locus_tag="VFX38_05310"
     CDS             complement(<1..521)
                     /gene="rpsC"
                     /locus_tag="VFX38_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004145350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEX5314306.1"
                     /translation="MGHKVNPIGFRLGVSAGWSSRWYAEGRRFAEYLHSDLKLRDYLK
                     KRLAGASVSRIEIERQAERVKVLVHTARPGMVIGKRGEDIETLTRDLRARLGTEVYVA
                     IEEIRKPELDAQLVAESIAQQLERRIMFRRAMRRAVGNAMRLGAHGIKVRVAGRLNGA
                     EISRSEWYREGRVP"
     gene            complement(532..873)
                     /gene="rplV"
                     /locus_tag="VFX38_05315"
     CDS             complement(532..873)
                     /gene="rplV"
                     /locus_tag="VFX38_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020561282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEX5314307.1"
                     /translation="METAARYRYARISPQKCRLVADQVRGKEAGEALNILTFSRKKAA
                     GLVRKALESAIANAEHNLGADVDELRVTAICVDEGPMFKRFQPRAKGRANQILKRTSH
                     ITVTVGDGADE"
     gene            complement(877..1182)
                     /gene="rpsS"
                     /locus_tag="VFX38_05320"
     CDS             complement(877..1182)
                     /gene="rpsS"
                     /locus_tag="VFX38_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_819286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEX5314308.1"
                     /translation="MPRSVNKGPFVDPHLAKKVSAAVAANSKRPIKTWSRRSMVTPDM
                     VGLTIAVHNGRQHVPVLITENMVGHKLGEFALTRTFKGHSGDRKAKAATTPAPAAGA"
     gene            complement(1195..2022)
                     /gene="rplB"
                     /locus_tag="VFX38_05325"
     CDS             complement(1195..2022)
                     /gene="rplB"
                     /locus_tag="VFX38_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018274068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEX5314309.1"
                     /translation="MAIRKAKPTSPGRRFVLEPTRDGLHKGRPEKSLVETQTRSGGRN
                     NQGRMTVRHQGGGHKRLYRRVDFRRNKDNVPARVERIEYDPNRSAHLALLLYADGERR
                     YIIAPRGVGPGDELQSGQHAPIRAGNALPLAGIPLGSEIHCVELQPGGGARLARSAGT
                     VIQLLAREGRYATLRLRSGEMRRVLVECRATLGSVSNSEHNLRSFGKAGAKRWRGIRP
                     TVRGVAMNPVDHPHGGGEGKTSGGRHPVSPWGKPTKGYKTRNNKRTDRMIVRRRGRK"
     gene            complement(2036..2335)
                     /gene="rplW"
                     /locus_tag="VFX38_05330"
     CDS             complement(2036..2335)
                     /gene="rplW"
                     /locus_tag="VFX38_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011041720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEX5314310.1"
                     /translation="MNADRLAELIRAPQISEKATRIAEAHRQIVFRVRPEASKPEIKR
                     AVEAMFEVKVEKVTTLRRQGKLGGRAMGRSGRHSDWKKAYVTLQPGFDIDFAGTE"
     gene            complement(2332..2502)
                     /gene="rplD"
                     /locus_tag="VFX38_05335"
     CDS             complement(2332..2502)
                     /gene="rplD"
                     /locus_tag="VFX38_05335"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX5314311.1"
                     /translation="MLIVTAQTSEALLLASRNLKAVDVRTVSELDPVSLIAFDKVVIT
                     VPALRELEKWLG"
BASE COUNT          373 a          868 c          784 g          484 t
ORIGIN      
        1 ggcacccgcc cttcgcgata ccactcgctg cgcgagattt ccgcgccatt caggcgcccc
       61 gcaacgcgca ccttgatacc gtgggcaccg aggcgcatgg cgttgccgac ggcgcggcgc
      121 atcgcgcggc gaaacatgat gcgacgctcg agctgctgcg cgatggattc ggcaaccagc
      181 tgagcatcga gttcgggttt gcggatctcc tcgatcgcga cgtacacctc ggtgccgagg
      241 cgcgcgcgca gatcccgcgt caaggtttcg atgtcctcgc cacgcttgcc gatgaccatg
      301 ccgggccggg cggtatgcac gagcaccttc acccgctccg cctgacgctc gatctcgatg
      361 cgactcaccg atgctcccgc gagccgcttc ttcaggtaat cgcgcagttt cagatcggaa
      421 tggagatact ctgcgaagcg acgtccttcg gcgtaccagc gcgaactcca gccggcggaa
      481 acgccgagcc ggaagccgat cggattgacc ttgtgtccca cgctcgctcc tctattcgtc
      541 ggcgccgtca ccgaccgtga cggtgatgtg gctcgtacgt ttcaaaatct gattggcgcg
      601 ccccttggcg cgcggttgaa agcgcttgaa catcggcccc tcgtccacgc aaatcgccgt
      661 gacgcgcagc tcgtccacgt ccgcgccgag gttgtgttcg gcattcgcga tcgccgattc
      721 gagcgccttg cgcaccaggc cggccgcctt cttgcgactg aaggtcagaa tgttcagcgc
      781 ctcgcccgcc tccttgcccc gtacctgatc ggcgaccagt cggcacttct gcggcgagat
      841 gcgggcgtag cgatagcggg cagccgtttc catgtatcag gctccagccg ccggcgccgg
      901 tgtggtcgcg gccttggcct tgcgatcgcc cgaatgcccc ttgaaggtcc gggtcagggc
      961 gaattcgccg agcttgtgcc ccaccatgtt ctcggtaatc agcaccggaa cgtgctgacg
     1021 tccgttgtgg acggcgatgg tgagccccac catgtcgggg gtgaccatcg agcgccgcga
     1081 ccaggtcttg atcggacgct tgctgttggc ggcgacggct gcgctgacct tcttcgccag
     1141 atgcggatcc acgaagggtc ccttgtttac ggagcgcggc atgatcccaa tctcctactt
     1201 gcgcccgcgc cgccgcacga tcatgcggtc ggtacgcttg ttgttgcggg tcttgtagcc
     1261 cttggtgggt ttgccccagg ggctcaccgg atggcggccg cccgaggtct tgccctcgcc
     1321 gccgccgtgc ggatgatcca ccggattcat cgcgactccg cgcacggtcg ggcgaattcc
     1381 gcgccatcgc ttggcgccgg ccttgccgaa cgaacgcaaa ttgtgctcgg aattgctcac
     1441 gcttccgagc gtcgcgcgac actcgacgag cacccggcgc atctcgccgg aacgcaggcg
     1501 aagcgttgcg taacggcctt cgcgcgcgag cagttgtatc accgtcccgg cgctgcgcgc
     1561 gagacgcgct cccccgccgg gttgcaactc gacgcaatgg atctcgctgc cgagcggtat
     1621 gcccgcgagc ggcagagcat ttcccgcgcg gatcggcgca tgttgccccg actgcaactc
     1681 gtcgcccggc ccgacgccgc ggggcgcaat aatgtagcgg cgctcgccgt ccgcgtacag
     1741 cagcagggcg agatgggcgc tgcggttcgg atcgtactcg atgcgctcga cccgcgccgg
     1801 cacgttgtcc ttgttgcggc gaaaatccac gcgtctgtac agacgcttgt ggccgccgcc
     1861 ctgatgacgc accgtcatgc ggccctgatt gttgcgcccg ccgctgcgcg tctgcgtttc
     1921 caccagcgac ttttcggggc ggcccttgtg cagaccatcg cgcgtcggct ccagcacgaa
     1981 gcgacgaccg ggggaagtcg gtttggcttt gcgaattgcc atgatccagt tcttcctatt
     2041 ccgtcccggc gaagtcgatg tcgaagccgg gttgcagggt cacgtaggcc ttcttccaat
     2101 ccgagtggcg cccgctgcgg cccattgctc gtccgcccag tttcccctgc cgccgcagcg
     2161 tcgtcacctt ctcgaccttg acctcgaaca tcgcctccac ggcgcgtttg atttcgggct
     2221 tgctcgcttc cggacgcacc cggaagacga tctgccggtg agcctccgcg atccgcgtcg
     2281 ccttctcgga aatctgcggc gcgcggatga gttcggcgag gcgatccgcg ttcatcccag
     2341 ccatttttcg agctcccgca gggccggcac cgtaatcacg accttgtcga aggcgatcag
     2401 gctcacgggg tcgagttcac tcaccgtgcg cacgtccacg gccttcaagt tgcgcgaagc
     2461 gagtagcaag gcctcgctcg tctgcgcggt gacgatcagc acatcggcc
//
LOCUS       DASUJG010000248         2810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1297433, whole genome shotgun sequence.
ACCESSION   DASUJG010000248 DASUJG010000000
VERSION     DASUJG010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297433"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VFX38_05340"
     CDS             <1..339
                     /locus_tag="VFX38_05340"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5314312.1"
                     /translation="IGFGLRNVTHKELALAEMQRVLAPGGRALVLEFSRPHEPLRRAY
                     ALYSFAVLPRLGRWVAKDEASYRYLAESIRRHPPQEELRKMMEAAGFERVRYYNQAGG
                     IVALHIGWKL"
     gene            336..941
                     /locus_tag="VFX38_05345"
     CDS             336..941
                     /locus_tag="VFX38_05345"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX5314313.1"
                     /translation="MSETRSLPAFLLDPASRALNAALAADPEALRTLRTLSGRRVAVE
                     ISDLSLVVPLAIAGERIELGGAAEGADATVSGRMSALIAAGRSGNPRGLTVAGDAEVV
                     QSLARVTSRLPRAAWERVATILGAGPSRALERLAGSLRKSLEGTRDRLSENVAEYLQY
                     EARLVVSRAELEDFLGEVDRLRADAERLAKRVERLRGGRRA"
     gene            938..2581
                     /gene="ubiB"
                     /locus_tag="VFX38_05350"
     CDS             938..2581
                     /gene="ubiB"
                     /locus_tag="VFX38_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEX5314314.1"
                     /translation="MIGPRQVLRFARINWVLVRYGLDGLILGAPWLRPLRFLRYLRPW
                     ALFQVRHESAPVRVRKALEDLGPIWVKLGQMLSTRADILPPAYAAELVKLQDQVAPFS
                     GRVARRRLEARFGRSIDALFASFDETPLASASIAQVHAARLPSGEEVVVKVLRPNVGR
                     VIDADLSLMYLFAALAERYLSEARRLHIREVIAQYDRTIHDELDLVREGSNAAQLQRN
                     FEDSPLLHIPKVYWEYTRSDVLVLERIGGIPIDDLAALRAAGVNLERLATNGVEVFFT
                     QVFRHNFFHADMHPGNLFVNASDSEFPVYIAVDFGICGTLAPEDQRYLVENFLAFFNR
                     DYDKVAALHVESGWIPKGTPVNEFAAAIRTVCEPIFNRPLKQISFAQLLVRLFQTARR
                     FDMEIQPQLTLLQKALLHIESLGRMVYPELDVWATGRPFLEEWMRERVGPRGIWNTIK
                     QELPDLGPILIETPKLLHTALTELLDGDLEEMGRKMTKHLRDEVVAERIARRRGHAGA
                     ALVIAGALLLGLSWQPSWAAWTIGACGLALVLFPTFRHG"
BASE COUNT          448 a          970 c          871 g          521 t
ORIGIN      
        1 atcggcttcg gcctgcgcaa cgtgacgcac aaagagcttg cgctcgcgga aatgcagcgc
       61 gtgctcgccc cgggcgggcg ggcgctggtg ctcgagttct cccgcccgca tgagccgctc
      121 cggcgtgcct acgcccttta ctccttcgcg gtgctgccgc ggctcggacg ctgggtcgcg
      181 aaggacgagg cgagttatcg ttatctcgcc gaatccatcc gccgtcatcc gccgcaggag
      241 gaacttcgca agatgatgga ggcggcgggc ttcgagcgcg tgcgctatta caatcaggcc
      301 ggcggcatcg tcgcccttca catcggatgg aaactgtgag cgaaacccgc agtcttcccg
      361 ctttcctcct cgatccggcg agccgggcgc tcaacgccgc cctcgccgcc gaccccgagg
      421 cgctgcgcac gctgcgcacg ctttccggac gccgcgtcgc ggtggaaatc agcgacctgt
      481 cgctcgtggt gccgctcgcg atcgcgggcg aacgcatcga actcggcggc gcggccgagg
      541 gcgcggacgc gaccgtgagc ggtcgcatga gcgcgctcat cgccgccggg cgaagcggca
      601 atccgcgcgg cctcaccgtc gccggcgacg ccgaggtcgt ccagagtctc gcacgggtga
      661 cgtcgcgcct gccgcgcgcc gcctgggaac gcgtcgcgac gatcctcggc gcgggccctt
      721 cgcgagcact cgaacggctc gcgggttcgc tccgcaaatc cctcgaagga acgcgcgatc
      781 gtctgagtga gaacgtagcc gaatatctcc agtacgaggc gagactcgtc gtatcgcgcg
      841 ctgaactcga agactttctc ggcgaggtgg atcggcttcg cgccgacgcc gaacggctcg
      901 cgaagcgcgt ggaacggttg cggggcgggc gccgggcatg atcggcccgc gccaggtgct
      961 gcgcttcgcg cgcatcaact gggtgctcgt gcgctatggg ctcgacggcc tcatcctcgg
     1021 tgcgccctgg ctgagaccgc tgcgcttttt gcgttatctc aggccctggg cgctctttca
     1081 ggtccggcac gaatccgcgc cggtgcgggt gcgcaaggcg ctcgaagatc tcggcccgat
     1141 ctgggtcaag ctcgggcaga tgctttcgac ccgcgccgac attctcccgc ccgcctacgc
     1201 cgcggagctg gtgaaactcc aggatcaggt ggcgcccttt tcgggacgcg ttgcgcgccg
     1261 acgcctcgaa gcgcgattcg ggcgcagcat cgacgctctg ttcgcctcct tcgacgagac
     1321 gccgctcgcc tcggcctcca tcgcgcaggt gcacgcggcg cgccttccaa gcggcgaaga
     1381 ggttgtcgtg aaggtgcttc gtccgaacgt cgggcgggta atcgacgccg atctttcgct
     1441 catgtatctc ttcgccgcgc tcgcggaacg ttacctctcc gaggcgcggc gccttcatat
     1501 ccgcgaagtg atcgcacagt acgaccgcac cattcacgac gaactcgatc tggtgcgcga
     1561 gggttcgaac gccgcgcaat tgcaacgcaa tttcgaggac tcgccgctcc tccacattcc
     1621 caaggtgtac tgggagtaca cccgctccga cgtgctcgtg ctcgagcgca tcggcggcat
     1681 tccgatcgac gatctcgcgg cgctgcgtgc ggcgggcgtg aatctcgaac ggctcgcgac
     1741 caacggcgtc gaggtatttt tcacgcaggt tttccgccac aacttctttc acgccgacat
     1801 gcatccggga aacctcttcg tcaacgcgag cgactccgag tttccggttt acatcgccgt
     1861 ggacttcggc atctgcggca cgctcgcgcc ggaagatcaa cgctacctcg tcgagaattt
     1921 cctcgctttc ttcaaccgcg actacgacaa ggtcgcggcg ctgcatgtcg agtcgggctg
     1981 gattcccaag ggcactccgg tcaacgaatt cgccgccgcg atccgcaccg tgtgcgagcc
     2041 gatcttcaac cggccgctca agcagatttc ctttgcgcaa cttctcgtgc gcctgttcca
     2101 gaccgcacgc cgcttcgaca tggaaatcca gccgcagctc acgcttctgc aaaaggccct
     2161 gctccacatc gagagcctcg ggcgcatggt ctatccggaa ctggacgtgt gggcgaccgg
     2221 gcgtcccttt ctcgaggaat ggatgcgcga gcgtgtcggc ccgcgcggca tctggaacac
     2281 gatcaagcag gaactgccgg atctcggccc gatcctgatc gaaacgccca aactccttca
     2341 caccgcgctc acggaactgc tggacgggga tctcgaggaa atggggcgaa aaatgacgaa
     2401 gcatctgcgc gacgaggtcg tcgccgagcg catcgcgcgc cggcgcggtc acgccggcgc
     2461 cgcgctcgtc attgccggcg cgctcctgct cgggctttcc tggcagcctt cctgggccgc
     2521 ctggaccatc ggcgcctgcg gtctcgcgct cgtccttttc ccgacgtttc gccacggctg
     2581 attacgccgc cgcatcgccg tcattctgag cgcactagac tgacccgatc tcaatacttt
     2641 gtcttcgctt ttcgtaatta tcccaatttg cggaaggaga ctctttcatg agactgtact
     2701 accacccgtt gtcttcgaat gcgcgcaagg tccgcctcgc ggcgaccttg ctcgacattc
     2761 ctctggagtt gcacaacgtc gaactgccca agggcgagca gcgcacaccc
//
LOCUS       DASUJG010000249         3507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_931739, whole genome shotgun sequence.
ACCESSION   DASUJG010000249 DASUJG010000000
VERSION     DASUJG010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931739"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..544)
                     /locus_tag="VFX38_05355"
     CDS             complement(98..544)
                     /locus_tag="VFX38_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314315.1"
                     /translation="MGNFKIEIEAVGGHGYDRNAKQGELIAFDGHPDEPDIAAKHFVD
                     EFSKHNSVLTATLTHWPDSTPIVDDLLTSKRVTSDFFSTANASAAAEPLLKYFTRDYN
                     DLGINRTIQHLAFMVVATMPRSAERTVALRKLLEFKDAAMRAANER"
     gene            complement(577..801)
                     /locus_tag="VFX38_05360"
     CDS             complement(577..801)
                     /locus_tag="VFX38_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314316.1"
                     /translation="MIEHQGHATLSTAYDGDVTITFPVGAQLTGYRLRDIRIRLSAAE
                     FVDPHVRAWLLEVVLPRRASVDEAVCIELG"
     gene            complement(938..1336)
                     /locus_tag="VFX38_05365"
     CDS             complement(938..1336)
                     /locus_tag="VFX38_05365"
                     /inference="COORDINATES: protein motif:HMM:NF012393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX5314317.1"
                     /translation="MNLEALRVAAGFSGSMTQNADNAPRTVRPVKGNVLVKQDPKSEK
                     VGKYGILFAPDGSEEWPSFGTVVAVGPGDVAEGGARIPMAVAPGDRIIFRRKPASALH
                     PDVREFDSDLEGMLMLPESYILAVVEDDGE"
     gene            1592..>3507
                     /locus_tag="VFX38_05370"
     CDS             1592..>3507
                     /locus_tag="VFX38_05370"
                     /inference="COORDINATES: protein motif:HMM:NF018125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-CBASS Acb1 family protein"
                     /protein_id="HEX5314318.1"
                     /translation="MSAITGIGDVTRDKRTTHTLAPIQMSYAMIAQLWAKEAMCAKAI
                     EKPGEECFRPGFELDIPGEQTGGKGGDSGKGDAFTDLKEYVTDQLRELDVVAKLKKAY
                     QYKRAYGGGALLLGINDGRRLDQPVDTTKNPSLDWINVLEPIEIFPISFYDDPAQANY
                     GEPEFYQLGAYAALPQFMGPIQEPKLNVFIHESRIIPFRGIKVSRYFFDNTVIDPYWG
                     ASLLPRFYDAMRDCAVAYSGAGLLSVDVSQPVITIEGLRALVGRNEEALRARMIALEQ
                     GRSIARAILLDGKEKYERQTTQLAGIPDLLDRCAQFLAANIDIPLSILFGYSPASLGT
                     PDNQELTQWYNLCRVIQTQEFTPPLRRIIDLIMRGRRSRGLPKRYDIAWGDLEHMTTT
                     QRVQNEFTQARTDSLNVKSGTIYPDEIRKSRWVGGHSFTTQINENKPAPGFMAPLPTG
                     VIAKADPATGVINAAEAKLVAGATGAANGNAAAGPPAGAHTVGGYTRRNPVRTGGEKQ
                     PGTGSGPEARASKADEAPGITAVGEVQHLHDRLAHARSTGTATPGVEAMFQKLLAITQ
                     AEAEQCAAGTCGPDCIYSHEAMDVAMGETITYAGFPVLVENPKGSVRHWVDSDGTTGS
                     TTMKWDYGEIPGSLG"
BASE COUNT          630 a         1201 c         1104 g          572 t
ORIGIN      
        1 ccgtgtccgg cgtgcgcctg cgcttcaagc gcagcgacac ggcgctcaag gcgatcgagc
       61 cgtcgcgcga ggagaccttg gccggtcgga agaaggctca tcgctcgttc gccgcgcgca
      121 ttgcagcgtc cttgaactcg agcaacttcc gcagcgccac cgtgcgctcc gcactccgtg
      181 gcatcgtcgc gacgaccatg aaggcgaggt gctgaatggt ccggttgatg ccgaggtcgt
      241 tgtagtcgcg cgtgaagtac ttgagcagtg gctcggccgc ggcgctggcg ttcgccgtcg
      301 agaagaagtc gctcgtgacg cgcttgctgg tcaggaggtc atcgacaatc ggcgtcgagt
      361 ccggccagtg cgtcaacgtc gccgtgagca cgctgttgtg cttgctgaac tcgtcgacga
      421 agtgcttcgc ggcgatgtcc ggctcgtcgg ggtgtccgtc gaaggcgatc aattcgccct
      481 gcttcgcgtt acggtcgtat ccgtgaccac cgactgcttc gatctcgatc ttgaagttgc
      541 ccattgtctt gtccttgtct ttcgttgttc ttgaaatcag cccaactcga tgcacaccgc
      601 ttcatccacc gaagcgcgcc gcggcaacac gacctcaagc aaccacgcgc gcacgtgagg
      661 gtcgacgaac tcggccgcgc tcaagcggat gcggatgtcg cggaggcggt atcccgtgag
      721 ctgcgcgccg acggggaagg tgatggtgac gtcaccgtcg tacgcggtac tcagtgtcgc
      781 gtgcccttga tgttcgatca ttcaactagt ccagtcctcc ggaatccgcg accgaacggt
      841 ccgatccaga aatgcaccga agcgcccttg ccttgcccag ccttgtcacg gcttgcctca
      901 ccacgcctgg cccgaccatg ccagaccacg gctggaacta ctcaccatcg tcctcgacga
      961 cagccagaat gtaactctct ggaagcatga gcatcccttc cagatccgag tcgaactcgc
     1021 gcacgtcggg atgcaacgcg ctcgccggtt ttcgacggaa gatgatccga tcacccggcg
     1081 cgaccgccat cggaatccgc gcgccgccct cggccacgtc gccggggccg acagcgacca
     1141 cggtgccgaa ggagggccac tcctcggagc cgtcgggcgc gaacaagatt ccgtacttgc
     1201 cgaccttctc ggacttcggg tcctgtttca ccagcacgtt tcccttgacg gggcgaacgg
     1261 tgcgtggtgc gttatctgcg ttctgcgtca tcgatccaga gaaccccgcc gccaccctga
     1321 gcgcttccag gttcacccct caggaaccct gagcgccctg aaccgccgcc ctctggccgt
     1381 tctctggact cgtgccacac tccatgcaca cggcgtgccg accaggaacg aaaaggggca
     1441 gtttgtcccg ggctcctctg gaaaccccgg tggagtacct cgtgcgcgca ccgacgccga
     1501 gccagagccc accgccctga ccgtacagga accgcccttc ctggcccaat tccgctcccc
     1561 ggacggcgtt ttcccccgcc gcgacgggtg gatgtccgcc atcacgggca tcggcgacgt
     1621 cacgcgcgac aagcgcacga cgcacaccct ggccccgatc cagatgagtt acgcgatgat
     1681 cgcgcaactc tgggccaagg aggccatgtg cgccaaggcg atcgagaagc ccggcgagga
     1741 gtgcttccgg cccgggtttg agctcgacat ccccggcgaa cagaccggcg gcaagggcgg
     1801 cgacagcggt aagggcgacg cattcaccga cctcaaggag tacgtcactg atcagttgcg
     1861 cgagctcgat gtcgtcgcga aactcaagaa ggcctaccag tacaagcgcg cgtacggcgg
     1921 cggcgcactc ctgctcggca tcaatgatgg ccggcgtctg gaccagcccg tcgatacgac
     1981 gaagaacccg tcgctcgact ggatcaacgt cctcgagccg atcgagatct tcccgatctc
     2041 cttctacgac gaccctgcgc aggcgaacta cggcgagccc gagttctacc agctcggcgc
     2101 gtacgcggcg ctgccgcagt tcatggggcc gatccaggaa ccgaaactca acgtcttcat
     2161 ccacgagtcg cggatcattc cgttccgcgg catcaaggtc tcgcgctact tcttcgacaa
     2221 caccgtcatc gacccgtact ggggcgcgtc gctcctgccg cggttctacg atgccatgag
     2281 ggattgcgcg gtcgcgtact cgggtgccgg gctcctctcc gtcgacgttt cgcagccggt
     2341 catcacgatc gaaggtctgc gcgcgctcgt tggacggaac gaggaggccc tccgcgcgcg
     2401 catgatcgcc ctcgagcagg gccggtcgat cgcgcgcgcc atcctcctcg acggcaaaga
     2461 aaagtacgag aggcagacca cgcagctcgc cggcatcccg gacctcctcg accggtgcgc
     2521 gcagttcctc gcggcgaaca tcgacatccc cttgtcgatc ctgttcgggt attcaccggc
     2581 gagcctcggc acgcccgaca accaggaact gacgcagtgg tacaacctct gccgcgtcat
     2641 ccagacacag gagttcacgc cgccgctgcg ccggatcatc gacctgatca tgcgcggccg
     2701 tcggtcgcga ggcttgccca agcgctacga catcgcgtgg ggcgacctcg agcacatgac
     2761 caccacgcag cgcgtgcaga acgagttcac gcaggcgcgc accgacagcc tcaacgtcaa
     2821 gtccgggacg atttaccccg acgagatccg caagtcgcgc tgggtcggtg gtcactcgtt
     2881 cacgacgcag atcaatgaga acaagcccgc gcccgggttc atggcgccac tgccgacggg
     2941 cgtcatcgcg aaggcggacc cggcgacggg cgtcatcaac gcggccgagg cgaaactcgt
     3001 ggccggcgcg accggcgcgg cgaacggtaa cgcggcggct gggccgcccg caggcgcgca
     3061 caccgtgggc ggctacaccc gtcgcaaccc ggttcggacc ggcggcgaga agcagccagg
     3121 aacgggtagc gggcccgagg cgcgcgcgtc gaaggcggac gaggcgcccg gcatcaccgc
     3181 ggtcggtgag gtgcagcacc tgcatgaccg cctcgcgcac gcgcggagca ccggcaccgc
     3241 gacgccgggt gttgaggcga tgttccagaa gttgctcgcg atcacgcagg cagaggccga
     3301 acagtgtgcc gcggggacgt gtgggccgga ctgcatctac tcgcatgaag cgatggacgt
     3361 cgcgatgggc gagaccatca cgtacgccgg gttcccagtc ctcgtcgaga atcccaaggg
     3421 cagcgttcgc cactgggtgg acagcgacgg cacgaccggc tcgacgacga tgaagtggga
     3481 ctacggcgag atcccgggct cgctcgg
//
LOCUS       DASUJG010000250         2023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_563397, whole genome shotgun sequence.
ACCESSION   DASUJG010000250 DASUJG010000000
VERSION     DASUJG010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563397"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VFX38_05375"
     CDS             complement(<1..237)
                     /locus_tag="VFX38_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(5-formylfuran-3-yl)methyl phosphate synthase"
                     /protein_id="HEX5314319.1"
                     /translation="MTHMLASVTGPAEAEVAVAGGADIIDLKDPSRGALGAVPIATVA
                     RTVAAVGQRRETSAVTGDLPMQPDLVVAAAKAMAG"
     gene            complement(234..764)
                     /locus_tag="VFX38_05380"
     CDS             complement(234..764)
                     /locus_tag="VFX38_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3280 domain-containing protein"
                     /protein_id="HEX5314320.1"
                     /translation="MEGSKRHVAMKSLQAVAVLAMTLALFSEAARADKSRAAIFPFEL
                     YDTSLQGEMQGPRKDEQVRLRHLGTQLADLLAQSGKYQPMNVPNMTGETLRTCGGCEV
                     DLARKAGAQVSVIGWVQKVSDLILNINIVMRSVATGKMVHAGSVDIRGNTDESWSRGL
                     AYLVRYRILADTAASR"
     gene            827..>2023
                     /locus_tag="VFX38_05385"
     CDS             827..>2023
                     /locus_tag="VFX38_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5314321.1"
                     /translation="MRNGLGRRTLLAKAGVVGATALAATVTPALADAALIRIGYIRST
                     DRKPTISLLDKPAPDDGLGGARVAIDDNNTTGTFMNQRFELSDVPVRPGADMRAALAK
                     LTGQDVHFVLIDLPAPDVLKLADAAKGASVVIFNVNAPDDSLRGADCRANVIHVAPSR
                     AMLADALAQYLLWKKWTRVLMIKGEHPDDALLADAYHRSVKRFGLRIVDERICKDTGG
                     SRQTDTGLLELQKNMPLLSQNAPEYDVLLTVDENEVFAGYLPYRTWDARPVVGSAGLE
                     PVSWSPSSQSFGGEQIQSRFERLNHRTMTPLDMQAWTAVRMVGETASRAGKLDFPTME
                     AYLKGPKFGVAAYKGKRLTLRDWDWQLRQPILLADGRVVVSISPQPGFLHQVTELDTL
                     GVDRPEA"
BASE COUNT          340 a          676 c          641 g          366 t
ORIGIN      
        1 ccccgccatc gcttttgccg ccgcgacgac caggtcgggc tgcatgggca gatcgccggt
       61 gaccgcgctc gtctcgcgcc gttgcccgac tgcggcgacg gtgcgcgcta cggtcgcgat
      121 tggcacggca ccgagcgcgc cgcgcgatgg gtccttcagg tcaatgatat ccgcgccgcc
      181 cgcgacggcg acctctgcct ccgccggccc ggtgacgctt gcgagcatgt gcgtcatcgc
      241 gatgccgccg tatcggcgag gatgcggtag cgcaccaggt aggcgaggcc gcgcgaccag
      301 gattcgtcgg tgttgcccct gatatcgaca ctgcccgcgt gcaccatctt gcctgtggcg
      361 acgctgcgca tgacgatgtt gatgttgaga atcaggtccg ataccttttg gacccatcca
      421 atcacagaga cctgcgctcc cgccttgcgc gccagatcga cctcgcaacc accgcaggtc
      481 ctcagggtct cgcccgtcat gttcggcaca ttcatcggtt gatatttgcc tgattgcgcc
      541 agcaggtcgg cgagctgcgt gccgaggtgc cttagccgaa cctgctcgtc ctttcggggg
      601 ccttgcatct ccccttgcag gctggtgtcg tagagctcga acggaaaaat cgccgcgcgc
      661 gacttgtcgg cacgcgctgc ctcggagaac aacgcaagcg tcatcgcgag cacggcaacg
      721 gcttgcaagg atttcatcgc cacatgccgc ttcgatcctt ccatccggcc ttgatgcgcc
      781 gtgcggagtt cttcatcctc tccccatgaa acgtccaaaa acgggcatgc gcaacggcct
      841 tggccgccgc accctgctcg ccaaggccgg cgtggtcggc gcgactgccc tcgccgcgac
      901 ggtaacccca gctctcgccg acgcggctct tattcggatc ggatacatcc gctcgaccga
      961 ccggaagcct accatttcgc tgcttgacaa gccggcaccc gatgacgggc ttggcggagc
     1021 aagggtcgcg atcgacgaca acaacacgac cggcaccttc atgaaccagc ggttcgagct
     1081 gagcgacgtt ccggtgcgac ccggtgccga catgcgggcg gcgctcgcga agctcaccgg
     1141 ccaggacgta catttcgtgc tgatcgatct cccggcgccg gacgtactca agctcgccga
     1201 cgctgcgaag ggcgcgtcgg tcgttatttt caacgtcaat gcaccagatg actcgctgcg
     1261 cggcgcggac tgtcgcgcga acgtgatcca tgtggcgcct tcccgcgcga tgctcgccga
     1321 cgcgctcgcc cagtacctgt tgtggaagaa atggacccgc gtgctgatga tcaagggtga
     1381 gcacccggat gacgcgttgc tcgccgacgc ctatcatcgc tccgtcaagc gattcggcct
     1441 gcgcatcgtc gacgagcgga tctgcaagga tacaggcggg tcccgccaga ccgataccgg
     1501 cctgctggaa ttgcagaaga acatgccgct gctgagccag aacgcgccgg agtacgatgt
     1561 cctgcttact gtggacgaga acgaggtatt cgcgggctac ttgccatacc ggacctggga
     1621 cgcgcgcccg gtcgtcggat cggcggggct ggagccggtg tcctggtccc cgagcagcca
     1681 gtccttcggc ggcgagcaga tccaaagccg cttcgagcgc ctcaatcacc ggaccatgac
     1741 gcctctcgac atgcaggcct ggaccgctgt gcgcatggtc ggcgagaccg ccagccgcgc
     1801 cggcaagctc gattttccga cgatggaggc gtacctcaag ggtccgaagt tcggcgttgc
     1861 ggcctataag ggcaagcggt tgaccctgcg cgactgggat tggcagctgc ggcagccgat
     1921 cctattggcg gatgggaggg tcgtcgtctc gatctcgcct cagccggggt tcctgcatca
     1981 ggtgaccgaa ctcgatacac ttggggtcga ccggccggag gcg
//
LOCUS       DASUJG010000251         8252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_75450, whole genome shotgun sequence.
ACCESSION   DASUJG010000251 DASUJG010000000
VERSION     DASUJG010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8252
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75450"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /gene="prpB"
                     /locus_tag="VFX38_05390"
     CDS             complement(<1..214)
                     /gene="prpB"
                     /locus_tag="VFX38_05390"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008608313.1"
                     /note="catalyzes the formation of pyruvate and succinate
                     from 2-methylisocitrate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEX5314322.1"
                     /translation="MLQESAGARFRAALEAEKPLQIVGTINAYAARLAERAGFRALYL
                     SGAGVANASFGLPDLGMTTLNDVCEDV"
     gene            complement(252..1349)
                     /gene="dinB"
                     /locus_tag="VFX38_05395"
     CDS             complement(252..1349)
                     /gene="dinB"
                     /locus_tag="VFX38_05395"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX5314323.1"
                     /translation="MQRKIIHVDMDAFYASVEQRDDPALRGRPVVVAWRGARSVVCAA
                     SYEARKFGVHSAMPATRAERLCPQAAFVAPDFVRYKAVSRLVREIFSRYSDLIEPLSL
                     DEAYLDVSAPKIVRPSATAIAQEIREAIRAETRLTASAGVAPNKYLAKIASDWRKPDG
                     LFVIRPTDVDAFLTPLAVGKLPGVGKVMRRKLADMNVMTIGELRTVPAARLEQGFGRW
                     GRRLYELARGIDEHAVEPHRPTLQVSAEDTFDRDLRLDELEAHIRRLAEKTWAAAERE
                     PGRIARTGVLKLKTGDFHVLTRSLTPPTRPRSAAELADIACALRDRVGRPARNLYRLV
                     GVGLAGFIDREDFAAQVSLFGSPEAKPERVG"
     gene            1550..2038
                     /locus_tag="VFX38_05400"
     CDS             1550..2038
                     /locus_tag="VFX38_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HEX5314324.1"
                     /translation="MKITILSVLLLGALATAPAPAFAAGSSAPSKAAIAQQFTIWNAA
                     LQTGDPAKVAALYCEPGGVLLPTVSNTVRTNRAAITDYFVHFLQLKPKGSIDQSYIHI
                     LGPDTAINSGIYTFDVTENGKPAQVQARFTFVYQKVNGTWCIMDHHSSAMPEKAPSAA
                     SH"
     regulatory      2090..2343
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2449..4257
                     /locus_tag="VFX38_05405"
     CDS             2449..4257
                     /locus_tag="VFX38_05405"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5314325.1"
                     /translation="MLRLFLWLAALFAATAAFARTPAPVLATHLSSITVTAPTGTPQA
                     LDNTLAPIILIGPQTLEAFPDTDIATLLQFHAGLDVANSGGPGQPVSLFLRGTGSAQT
                     LVLVDGIRVNRGAIGGPALANIFTSSIERVEVVEAPRSALYGSDAIGGVVSITLKQPP
                     RRGFDGGAVLSGGRYATRGADVHAGGGNDSFFGGADMNWFETAGFPPEVASDNASAYR
                     NQSLHAVMGAQNDTANAYATFWRSSGTTNYLDFALNPASEDYTDRVGSVHLERTMSSN
                     WQSRVILGQFFDDIQQLQSSDYTRTWRNSLDWRNDLSLGTHQLLSVGAYLAHEHVSAL
                     SFGTGYDEITRTQALYAQDQLAFGPHQVILAARESHFSSFGNQFTWNADYGLRFAREW
                     RFTAGAGTGFRAPTATDRFGFGGNPDLKPEYSHAYNLGLYRDFGSHAVLGLQLYRNRI
                     ADLITFEATPGGGQEANIGRATITGANLSLQAVYGRWRIEPAFSFQRPLNDETGGYLP
                     RRSRKSATLDLAYDTGRWQAGLYVLASGPRKDSDFTAVTDAGYVLTDLTGAVAFAPHW
                     RLRARIENVFDVHYQSVAGYANAGRGLYLALSYDVK"
     gene            4419..4994
                     /locus_tag="VFX38_05410"
     CDS             4419..4994
                     /locus_tag="VFX38_05410"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX5314326.1"
                     /translation="MGNRLSRIYTRTGDDGTTGLVGGARVPKTGARVEAYGTVDELNS
                     AIGLVLAFAIPAAVRECLLRVQHELFDLGGEFATPGATTIKAESVARLESELDEFNAT
                     LPPLKEFILPGGGQAAAACHLARAVARRAERRAFALAGAEETNPESLRYLNRLSDLLF
                     VLCRILTRAAAGEEILWQSARLKAQMKKGDA"
     gene            complement(5115..7604)
                     /locus_tag="VFX38_05415"
     CDS             complement(5115..7604)
                     /locus_tag="VFX38_05415"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314327.1"
                     /translation="MNRRSTIRFGILVFSLVLTAGGAIALAATPSPSMVGTATRPAHT
                     SQALPAALGPVLYQGFAQIAGATYAINKNGCAALPRQTLKACFDGSGAHFTGAGSPLA
                     LRLTSWGRGNDLASVSRVRPKINGNEASYFHGDLTEWWRVLPIGFEQGFTIAKRPSGN
                     GELTLTLALAASRQDAGVPRNGSLTWGKLRYGQLVVTDAKGKIVSATLQTKGDRVLIA
                     VNDEYASYPLTVDPLLWLEHEVTANDGTANNNFGISVAVSGTTAVIGAPTATVDGNRH
                     QGAAYVFSESNGSWTQTAMLTAIDGTKNAEFGVAVTLSGTTAFIGADSTVDGNKDQGA
                     VYVFNESNGNWTQTQKLIASDGVEYGFFGSAVALSDTTALIGAWCDGYLPDVGCNPGA
                     AYIFNESNGTWAQVQKLTASDGQVSDSFGAAVALDGTTALIGADGFNDFQGEAYVFGE
                     SGGAWTQKAELAASDAAEGDDFGGTVALSATTALIGAECAPIALFCGTGEAYVFGESN
                     GSWTQTQKLVASDGAQSDEFGYSVALDGATALVGAIYAAINGNAEQGAAYVFTRSGGI
                     WTQTQKLTASDGAAFRVFGSAAALDGTTALVGADSSIVVRDGQDVAYFYSQSDLDLNL
                     NAPQTIGQGQDYVSQTVATNNASAASPAVTATVSVPAAASFISATATQGSCSEASRLV
                     TCDFGPIPGNAGTATVNVSLKATGSPGTAIENTASIALAEPALTASAPTQIIQTTTCP
                     ARYTTYTGNLNPNAWFRTPTYQAPAGEENAILTEPTAFELYVQYRNGRVQKTYRIPGN
                     EIHRQAPAGTYQWGVKAGDTGGAYTLCIMHP"
     gene            complement(7843..>8252)
                     /locus_tag="VFX38_05420"
     CDS             complement(7843..>8252)
                     /locus_tag="VFX38_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEX5314328.1"
                     /translation="RERLRRKYAQDPPLRVFYEISAKPLYTVGGGQIISQILALCGGR
                     NIFAKLTTLAPVVDEGAVVARNPQAIFTDDSPGAAGRLDDWRRWPWLAAVKADNLFVL
                     PADLIGRAGPRILKGAAIVCRDLSIVRSRAGKS"
BASE COUNT         1493 a         2784 c         2508 g         1467 t
ORIGIN      
        1 gcacgtcctc gcacacgtcg ttgagcgtcg tcatgccgag gtcgggcagg ccgaaggagg
       61 cattcgcgac cccggctccg gaaagataaa gtgcccggaa gcccgcgcgc tcggcgagcc
      121 gcgccgcata agcgttgatc gtgcccacga tctgcagcgg cttttcggct tcgagcgcag
      181 cgcgaaagcg ggcgccggcg gattcctgca acattttcga gtccggtcgg gcctttgttt
      241 tcccgcccat tttatccgac ccgttccggc ttcgcctccg gcgaaccgaa aagcgatacc
      301 tgggccgcaa aatcttcgcg atcaatgaag cccgcgaggc cgacacccac caatcggtag
      361 agattgcggg cggggcggcc gacgcggtcg cgcaacgcgc aggcgatatc ggcgagttcc
      421 gcggccgagc gcggacgtgt cggcggcgtg agactgcggg tcagcacgtg aaaatccccc
      481 gtcttgagtt tgagcacccc ggttcgcgcg atacggccgg gttcccgctc cgccgcggcc
      541 caggtctttt ccgcgagacg acggatatgt gcttcgagtt cgtcgagacg cagatcgcga
      601 tcgaaggtgt cctccgccga cacctgcagc gtcgggcgat ggggctcgac ggcatgctcg
      661 tcgatgcctc gcgcgagttc gtagaggcgc cggccccagc gcccgaaccc ctgttcgagt
      721 cgcgcggccg gcacggtgcg caattcgccg atcgtcatga cattcatgtc ggcgagtttg
      781 cgccgcatca ccttgccgac gcccggcagc ttgccgacag cgagcggcgt caaaaaggca
      841 tcgacgtccg tcgggcggat gacgaaaagc ccgtcgggtt tgcgccagtc cgatgcgatc
      901 tttgcgagat acttgttcgg cgcgactccg gccgaggccg tgagtcgcgt ttcggcacga
      961 atggcctcgc gaatttcctg cgcaatcgcg gtcgccgaag gccgcacaat tttgggcgcg
     1021 ctgacgtcca gataagcctc gtccaatgac agcggctcga tgagatcgga atagcgcgaa
     1081 aagatttcac gaaccagccg cgacaccgcc ttgtagcgaa cgaagtccgg cgccacgaat
     1141 gctgcctgcg ggcacagccg ttccgcgcga gtcgccggca tcgcggaatg aacgccgaac
     1201 ttgcgcgcct catagctcgc cgcacacacg accgagcgcg cgccgcgcca ggcgacgacg
     1261 accggacggc cgcgcagcgc gggatcgtcg cgctgctcca cggaggcgta gaacgcgtcc
     1321 atgtccacgt gaatgatctt gcgctgcaac ggagagttca aaaccgagtg tccggcggcc
     1381 aggacggaac gggtctagga tagccttcgc gggtttgctg cgctttacgc cagagtcggg
     1441 ggacgcgcga tcgcgtggcc gacgcgagcg gggcacgccg caggacgctc tgctatgctc
     1501 ttgccgcccc aatccccgat cctttccact tcacctccaa ggaatcgcca tgaaaatcac
     1561 catcctttcc gtgctccttc ttggcgcact tgccaccgca ccggcaccgg cctttgccgc
     1621 cggcagcagt gctccgagca aagcggccat cgcgcaacaa ttcacgatct ggaacgccgc
     1681 tttgcagacg ggagatcccg ccaaggtcgc cgcactctat tgcgagcccg gcggcgtgct
     1741 tctcccgacc gtgtcgaaca ccgtgcgcac gaatcgcgcg gccatcaccg actacttcgt
     1801 acacttcctg cagctcaagc cgaagggcag catcgatcag tcctacatcc acatcctcgg
     1861 tcccgacacc gccatcaatt ccggcatcta cacctttgac gtgacggaaa acggcaagcc
     1921 cgcgcaagtc caggcccgct tcaccttcgt gtaccagaag gtgaacggca cgtggtgcat
     1981 catggatcat cactcctcgg ccatgccgga aaaggccccc tcggccgcgt ctcactgacg
     2041 gaacgggcgc gggtgttctt gtccgcttga cgcgacgcac ttccgccccg ataatggcgt
     2101 tgccgctggc gtcctgcttt caaggcagga ctgggaagcc ggtgcgcccg ctcgatgcgg
     2161 gcaattccgg cgctgccccc gcaacggtag gcgagtcaat cgcccgtcac tgaagccact
     2221 gcgcgaacac gacgcgcggg aaggcgacgg gcgaaggcct ctcaccctga ccctctcccg
     2281 gagggaaaag ggaaagaagg ccgctcgcga gcccggagac cggccacggc acaggtgtcg
     2341 gtgccgcggc gggtggcttc cggagctttt tgcgcttcct tacttcctcc gcaaaccgat
     2401 tggctcttct cgcccgcacg cggggtcggc ttgcgcgagg tacacccaat gttgcgtctg
     2461 tttctttggc tggcggcctt gttcgcggca acggcggcgt tcgcccgaac gcctgcacct
     2521 gtccttgcca cgcacttgag ttccatcacc gtcacggcgc cgaccggcac cccgcaggcg
     2581 ctggacaata ccctcgcccc gatcattctg atcggcccgc agacactcga agcctttccc
     2641 gacaccgaca ttgccaccct gctccagttt cacgccgggc ttgacgtcgc gaacagtggc
     2701 ggtccggggc aaccggtctc gctgtttctc cgcggcacgg gcagcgccca gacgctggtg
     2761 ctggtggacg gcatccgcgt caaccgcggc gccatcggcg gcccggcgct cgcaaacatc
     2821 ttcacctcga gtatcgagcg cgtggaagtg gtcgaggcac cgcgctcggc tctgtacggt
     2881 tcggatgcga ttggcggagt ggtttcgatt acgctcaagc agccgccgcg ccgaggtttc
     2941 gacgggggcg cggtgctcag tggcggccgt tacgcgacgc gtggagccga cgtgcacgcg
     3001 ggcggcggca acgacagctt cttcggcggg gccgacatga actggttcga gaccgccggc
     3061 tttccgccgg aggtcgcaag cgacaacgcg agcgcctatc gcaatcagag cctgcatgcg
     3121 gtgatgggag cacagaacga caccgcgaac gcctatgcaa cattctggcg ctcgtccggc
     3181 accaccaact acctggactt cgcgctcaat ccggcgagcg aggattacac cgaccgcgta
     3241 ggaagcgtgc atctcgaacg caccatgagc agcaactggc aaagccgcgt gatcctcgga
     3301 caattcttcg acgacattca acagctccaa agctccgact acacccgcac ctggcgcaac
     3361 tcgctcgact ggcgcaacga tctcagcctc ggcacgcacc agttgctcag cgtcggcgca
     3421 taccttgccc acgaacatgt gagcgcactc tcattcggaa ccggctacga cgaaattacc
     3481 cgcacgcagg cgttgtacgc gcaggaccag ctcgcattcg gcccgcatca ggtgatactc
     3541 gccgcgcgcg aatcgcattt ctccagtttc ggcaaccaat tcacctggaa cgcggactac
     3601 ggcctccgtt tcgcccgcga gtggcgtttc accgccggcg ccggcacggg attccgcgcg
     3661 ccgacggcga ccgaccgctt cggcttcggc ggcaacccgg atctcaaacc cgagtattcg
     3721 cacgcgtaca atctcgggct ctaccgcgac ttcggcagcc acgccgtgct cggacttcag
     3781 ctttatcgca accggatcgc cgacctcatc acattcgagg cgacgccggg cggcggacag
     3841 gaagcaaaca tcgggcgagc gacgatcacc ggcgccaatc tttccctgca agcggtgtac
     3901 gggcgctggc ggatcgaacc cgctttcagc tttcagcgcc cgctgaacga cgagaccggc
     3961 ggctatctgc cgcgccgttc ccgcaaaagc gcgacactcg atctcgccta cgacaccggc
     4021 cgctggcagg ccggcctcta cgtgttggca agtggcccgc gcaaggactc ggacttcacc
     4081 gccgtgaccg acgcgggcta tgtgctgacc gatctcaccg gcgcggtcgc gttcgcccct
     4141 cattggcggc ttcgcgcccg catcgaaaac gtcttcgacg tgcactacca gtccgtcgcc
     4201 ggctacgcga acgccggcag agggctgtat ctggccttaa gctatgacgt gaagtagctg
     4261 gccgctgaaa ctgctgcgct cggcggcttg cacgctagcg gtgctcggaa tcctcgttta
     4321 ctttccgtgt aaactgcggt tcctgcgctc ccgccgcacg caatccgcct tcgctcgcta
     4381 cgtttcagca gcctgccaca ggagacaggg agacagacat gggaaaccgc ctgagccgca
     4441 tctacacccg caccggcgac gacggcacga ccggtctcgt cggcggcgcg cgcgttccga
     4501 agacgggcgc gcgcgtggaa gcctacggca ccgtggacga actcaactcc gccatcgggc
     4561 tcgtactcgc cttcgcgatt cccgccgcgg ttcgcgaatg cctcctgcgc gtgcagcacg
     4621 aactgttcga cctcggaggt gagttcgcca caccgggcgc aaccacgatc aaggccgaat
     4681 ccgtcgcgcg gctcgaatcc gaactcgacg aattcaacgc gacgctcccg ccgctcaagg
     4741 aattcattct gcccggcggt ggccaggccg cggccgcctg ccatctcgcg cgcgccgtcg
     4801 cccgccgcgc cgagcggcgc gccttcgcgc tcgccggcgc tgaggaaaca aatcccgaaa
     4861 gcctgcgtta tctcaaccgc ctttccgatc ttctcttcgt gctttgccgc atattgaccc
     4921 gcgccgccgc cggcgaggaa atcctctggc aaagcgcccg cctcaaggcg cagatgaaaa
     4981 aaggcgacgc ctgaaccgga gtcacgcgcc gccatagaag ataccggaaa cccgatcaat
     5041 tgggtgagta atacacgggg gtttccacat gggcgccctg gttggcgaca atccggatta
     5101 cgcggttcaa tgtcttacgg atgcatgatg cacagcgtgt aggcgccgcc ggtgtccccc
     5161 gccttcacgc cccattggta ggtacccgcc ggtgcctggc gatgaatctc atttcccggg
     5221 atgcggtacg tcttctgaac ccttccattc cggtactgca catagagttc gaaagccgtc
     5281 ggctccgtga ggatcgcgtt ctcctcgcct gcgggcgcct gataggttgg ggtgcggaac
     5341 caggcgttcg gattcagatt gccggtgtaa gtggtgtagc gcgccgggca agtcgtggtt
     5401 tgaataatct gggtcggggc gcttgccgtc agcgccggtt cggccagagc gatgctcgcg
     5461 gtgttctcga tcgcggtgcc gggcgagccc gtcgccttta agctgacgtt gaccgtcgcc
     5521 gtgcccgcat taccaggaat cggcccaaag tcgcaggtca ccaggcgcga tgcttcgctg
     5581 caactgccct gcgtggcggt ggcggatatg aagctcgccg ccgccggcac ggagaccgtc
     5641 gcggtgactg cgggcgatgc ggcactcgca ttattggtcg cgaccgtctg gctgacgtag
     5701 tcttgcccct gcccgatcgt ctgtggcgcg ttcaggttca agtcgagatc actctgactg
     5761 taaaagtagg ctacgtcttg tccgtcgcga acaacgatgg aggagtcagc accgacgaga
     5821 gcagtcgtac catccagagc ggctgcggag ccgaagacgc ggaaggccgc gccgtcgctc
     5881 gccgtcaatt tctgcgtctg tgtccaaatg ccaccagacc gggtaaacac ataagcagcg
     5941 ccctgctccg cattcccgtt gatggctgca tagatcgcgc cgaccagggc ggtcgcgccg
     6001 tcgagggcga ccgaatagcc aaattcatcg ctttgcgctc catcgctcgc gaccaacttt
     6061 tgcgtctgcg tccagcttcc attcgattcg ccgaacacat aggcttcgcc cgttccgcag
     6121 aagagagcaa tcggggcgca ctccgcgcca atgagggcag tcgtggccga aagggccacc
     6181 gtgccgccaa agtcatctcc ctcggcggcg tcgcttgcgg caagttcggc cttctgtgtc
     6241 catgccccgc ccgattcgcc aaatacatag gcctcaccct ggaaatcgtt gaacccgtcc
     6301 gccccaatga gcgcagtcgt gccgtcgagg gcgaccgcgg caccgaagga atcactcacc
     6361 tgaccgtcgc tggcggtgag tttttgcacc tgcgcccaag ttccattcga ctcattgaat
     6421 atataggcgg cgcccggatt gcagcctacg tcaggcaagt aaccatcaca ccatgcgcca
     6481 atgagggcgg tagtgtcaga aagggcaacc gcagagccga aaaaaccata ctcaacgccg
     6541 tcgctggcaa tcagtttctg agtttgcgtc cagttcccgt tcgactcgtt gaacacatag
     6601 accgcgccct gatctttgtt accgtctacc gtggaatcgg caccgataaa cgcggtcgta
     6661 cccgaaagcg tgaccgccac gccgaattcg gcattcttcg ttccgtcaat cgcggtgagc
     6721 atggccgtct gcgtccagct tccattcgat tcgctgaaca cataggctgc tccttggtgg
     6781 cgattaccat cgacggtagc agtcggcgcg ccgatgactg cggtcgtgcc cgaaacggca
     6841 acggaaatgc caaaattgtt attcgccgta ccgtcgttgg cggtgacctc gtgctcgagc
     6901 cacagcagcg gatccacggt gagcggatag ctggcgtact cgtcgttcac cgcgatcagc
     6961 acgcgatcgc ctttcgtttg cagcgtcgcc gacacgattt tccctttcgc atcggtcacg
     7021 acaagctgcc catagcgcag tttgccccac gtcaagctgc cgttcctggg aacgccggca
     7081 tcctgccggc tcgcggccag cgccagcgtc agcgtcagtt cgccgtttcc gctgggccgc
     7141 ttcgcgatcg tgaagccctg ttcgaagccg atcggcaaca cccgccacca ttcggtcaga
     7201 tcgccgtgaa agtagctcgc ctcattgccg ttgatcttcg gtcgtaccct actgacggaa
     7261 gcgagatcgt tcccgcgtcc ccagctcgtg agccgcaatg ccagcggcga gccagcgccg
     7321 gtaaaatgcg cgccgctgcc gtcgaagcaa gccttgagcg tctgccttgg cagtgcggca
     7381 cagccgtttt tattgattgc gtaggtcgcg cccgcaattt gcgcaaaccc ctgatacagc
     7441 accgggccca acgccgcggg caacgcctgt gaggtgtgag cagggcgggt cgcagtcccc
     7501 accatggagg gactcggcgt agcggccagc gcgatcgcgc cgccagccgt cagcaccaac
     7561 gaaaacacca gtatcccgaa acggattgtg gaacgcctgt tcatgggact ctccttccca
     7621 gaccgtggat cgattccgga gcgccgggcc gcgcacgcga agggatatcg gagagggtcc
     7681 acccggcagg tcattgagtc ggcgaccgag cgggccagga ccgaaacgag cctttgtgtg
     7741 gattcaattc tacaaccgca cgcaaggcct tatcaagaat tcaaagcctc cctgatttct
     7801 gtcgtgaagc cgtcgctccc gtcggtcgca tgcgcaggga gttcaagatt ttcccgcccg
     7861 ggaacggacg atcgaaaggt cgcggcagac gattgcggcg cctttgagga tgcgcggccc
     7921 cgcgcgtccg atgagatcgg cgggcagaac gaaaagattg tccgccttca ccgcggcgag
     7981 ccagggccag cgccgccagt cgtcgagccg cccggccgcg ccgggcgaat cgtcggtgaa
     8041 aatcgcctgc ggattgcggg cgacgacggc accctcgtcc acgaccggcg cgagcgtcgt
     8101 caatttcgcg aagatgtttc gcccgccgca gagcgcgagg atctggctga tgatctgccc
     8161 gccgccgacg gtgtaaagcg gcttcgccga tatctcgtaa aacacccgca ggggcggatc
     8221 ctgcgcgtac ttgcgccgga ggcgctcgcg ct
//
LOCUS       DASUJG010000252         2167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_931992, whole genome shotgun sequence.
ACCESSION   DASUJG010000252 DASUJG010000000
VERSION     DASUJG010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931992"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VFX38_05425"
     CDS             <1..386
                     /locus_tag="VFX38_05425"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5314329.1"
                     /translation="GTIFHRVVPGFVIQGGGFTTALQEKPTRPPIADEAANGLANLRG
                     TIAMARKRNPDSATSQFYINLADNPMLDFRFPDNPGYAVFGHVVSGMDVVERIAKQPT
                     GAGGPFSTGVPATPIVIEQTKLLAP"
     gene            383..1108
                     /locus_tag="VFX38_05430"
     CDS             383..1108
                     /locus_tag="VFX38_05430"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEX5314330.1"
                     /translation="MSSLFNSDLHLDAARPAITALFLEFLAGEARAAETLYILGDLFE
                     AWIGDDDPDPHHAEVAAALASLTAAGVPVRFLVGNRDFLLGENYAARAGMEILCEPVT
                     LNLHGRPTVVLHGDVLCTDDRDYQEFRATVRNPGWQRAFLARPVAERRAAATAARAES
                     RRRLAGKAPEIMDVNEAAVAAMFREQAVTRMIHGHTHRPAMHRLEVDGRSCERIVLGD
                     WYEQGSVLHVDDAGIELKSLALS"
     gene            complement(1136..1333)
                     /locus_tag="VFX38_05435"
     CDS             complement(1136..1333)
                     /locus_tag="VFX38_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314331.1"
                     /translation="MIVFLPFLWLALWLIGAIAVAAFSGLAPAGAGLTLFLAIFLGGA
                     IGLPLVGGAIWYVGRRLREGD"
     gene            complement(1403..>2167)
                     /locus_tag="VFX38_05440"
     CDS             complement(1403..>2167)
                     /locus_tag="VFX38_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314332.1"
                     /translation="KDYRADQAQNLYYNLGDKLANVAFEHPDSLEPVAKALGLEVQTI
                     AGVTRDQGKDVAGKEAIRAAAFSSQVLKDHQNSEPVKLGKDDAVVLRVAKETPAHPRP
                     LAKVHDDIAKTIEGNRAQAAAKAAASGAVKDLQAGHEAGAVASALGTKLQGPSSVARS
                     DPKVPAAILEAAFSTPPRADGKSRFGTAALTNGDQAVYAMLGVVPQTSANKEQAAMVQ
                     LYGRQLAQINANEAYDSYLAWLRARADIKIEKQNIP"
BASE COUNT          345 a          739 c          704 g          379 t
ORIGIN      
        1 atggcaccat ctttcaccgg gtggttcccg gcttcgtgat ccaggggggc gggttcacga
       61 ctgccttgca ggaaaaaccg acccgcccgc ccattgccga cgaggcggcg aacggcctcg
      121 ccaatctgcg cggaaccatc gcgatggcac gcaagcgcaa cccggattcc gcgacttcgc
      181 agttctacat caatctcgcc gacaacccga tgctcgactt ccgctttccg gacaatccgg
      241 gctacgcggt cttcggccat gtcgtctccg gcatggatgt ggtggagcgg atcgccaagc
      301 agccgaccgg cgcgggcggg ccgttttcga cgggcgtgcc ggcgacgccg atcgtcatcg
      361 aacagacgaa actcctcgcc ccgtgagcag cctgttcaac tccgatcttc atctcgatgc
      421 ggcccgcccg gcgatcacgg cgctgtttct cgagtttctc gcgggcgagg cgcgcgcagc
      481 cgaaacgctc tacatcctcg gcgatctctt cgaggcctgg atcggcgacg acgatcccga
      541 tccgcatcat gccgaggtcg ccgcggcgct cgcgagtctg accgcggccg gcgtgccagt
      601 acgatttctc gtcggcaatc gcgatttcct cttgggggag aactacgcag cgcgcgcagg
      661 aatggaaatc ctctgcgagc cggtgacgct gaatctccac ggcaggccga ccgtggtcct
      721 gcatggagac gttttgtgta ccgacgatcg cgactaccag gagtttcgcg cgacggtgcg
      781 caatcccggc tggcagcggg cctttctcgc gcggcccgtg gccgagcggc gggcggcagc
      841 tacggcggcg cgcgccgaaa gccgccggcg tctcgcgggc aaggcgccgg aaatcatgga
      901 tgtcaacgag gcagccgtcg ccgcaatgtt tcgcgagcag gccgtgacgc gcatgattca
      961 cgggcacacc caccgccccg cgatgcatcg tctcgaagtc gatggcagga gctgcgagcg
     1021 catcgtgctc ggcgactggt acgagcaggg cagcgtattg cacgtggacg acgcgggaat
     1081 cgaactcaag tcactcgcgc tttcctgagc gctgccgccg ccggcgcggc cgcggtcaat
     1141 cgccttcgcg cagccgcctt ccgacgtacc agatcgcgcc gccgacgagc ggaaggccga
     1201 tcgctccgcc aagaaatatc gcgagaaaca gcgtcaggcc cgcgcccgcg ggcgcgagac
     1261 cgctgaacgc cgccacggcg atcgcgccga tgagccagag cgcgagccag agaaacggga
     1321 ggaaaacgat catgttgctc tgcaccgccg ctctggcaag ctctgcgaca tcaaccaatc
     1381 ttcccgtcgg cggtccgcag gatcagggga tgttctgctt ttcgatcttg atgtcggcgc
     1441 gcgcgcgcag ccaggcaagg tagctgtcgt atgcctcgtt cgcattgatc tgcgcaagct
     1501 gccgaccgta cagctgcacc atcgccgctt gttccttgtt ggcggatgtc tgcggaacca
     1561 cgccgagcat cgcgtacacc gcctgatcgc cgttggtgag ggcggcggtc ccgaagcggc
     1621 tcttgccgtc cgcgcgcgga ggcgtcgaga atgcggcctc gaggatcgcc gcgggcacct
     1681 tgggatcgct gcgggcaacg gaactcggtc cctgaagctt ggttccgagc gcgctcgcga
     1741 cggcgccggc ctcgtgaccg gcctgcaaat ctttcacggc gccggacgcc gcagccttcg
     1801 ccgccgcctg cgcccgattg ccttcgatgg tcttcgcgat gtcgtcgtgc accttcgcga
     1861 gcgggcgcgg atgcgcgggc gtttccttcg cgacccgcag caccaccgcg tcgtccttgc
     1921 cgagcttgac gggttcgctg ttctggtgat ccttcagcac ctgcgaactg aacgcagccg
     1981 cgcggatcgc ctccttgccc gcgacatcct tgccctggtc gcgggtcacg cccgcgatcg
     2041 tctgcacctc gagcccgagc gccttggcca cgggttcgag cgaatccggg tgctcgaagg
     2101 cgacgttggc gagcttgtcg ccgagattgt aatagagatt ctgcgcctga tccgcgcggt
     2161 agtcctt
//
LOCUS       DASUJG010000253         3390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1542995, whole genome shotgun sequence.
ACCESSION   DASUJG010000253 DASUJG010000000
VERSION     DASUJG010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1542995"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..159
                     /locus_tag="VFX38_05445"
     CDS             1..159
                     /locus_tag="VFX38_05445"
                     /inference="COORDINATES: protein motif:HMM:NF014009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HEX5314333.1"
                     /translation="MSAHARTLLAGEAVLVPVRDVKLGLGRWQGIFLWEHRRGAHRRE
                     VLITVIGD"
     gene            complement(185..700)
                     /locus_tag="VFX38_05450"
     CDS             complement(185..700)
                     /locus_tag="VFX38_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HEX5314334.1"
                     /translation="MLARLHRFITAVMILALAPLAFAAATSAANQKMVAHGRHLVMIT
                     GCNDCHTPGYGNEAGQVPERLWLTGERIGWLGPWGTTYAVNLRLLAASLDEKQWIEQL
                     RTTKARPPMPWYVFASMSNYDLASIYRYLRALGPAGGPAPAWLPPGKKPSPPYFEFGP
                     PAAPPPAAGTH"
     gene            762..2219
                     /locus_tag="VFX38_05455"
     CDS             762..2219
                     /locus_tag="VFX38_05455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HEX5314335.1"
                     /translation="MAPDETLPRDSAAASDFWGHPRLLWMLLAVTVGLNFAFYGFRAF
                     LAPYAAEAFFSGLPQAEALKQANFLFAGFGALTYATAILGGWVADNMLGEVQALRISL
                     WLATLGILALAWPTREGFTLALAFFVLAAGLGIPLTVLIGRNYGKGDSRRDAGYTLFY
                     LAINLGGFIAPFICAAWIGVHYGYRWGFIAAACGMAFGALVFELRHRRMSGAREKVRF
                     QRRWSAPAVIVAMLVLLWPCAWLLAHPTEMTIVVYALMGLLVLYFVGSSLRRRDRVQA
                     HRYLAMLILFVAMVLFWAWSLESASALNFFARDYVDAPFNFTLFQSVNPLYILILAVP
                     LSLLWPWLDRRGINPSTPRKFGIGLLLVALGYGVLLAATHFLVGADGHIDWWPLMLWY
                     LFATLGELALSPIGYAMIGNLAASDEASIAMGGWFFGVSVSYDLAGQIAAMTTSGGIA
                     GYSQVFAGLFVVGVAIALLFLVAAPWIVKLMHGVR"
     gene            complement(2256..>3390)
                     /locus_tag="VFX38_05460"
     CDS             complement(2256..>3390)
                     /locus_tag="VFX38_05460"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX5314336.1"
                     /translation="LYASGDPVPFDIIAGTSAGAMSAAYLATQMPDYRKASAALAALW
                     SALRVKDVYSSDYRKIFAVVARWLWAFVSGGAGESNPRSLLDNGPLRKLLDENLEFSA
                     IQRNIDAGLLRGLTITIAGYSSERSLTYFQAAPDVEPWWRERREGRPVMMTLDHIMAS
                     LALPIVFPAVRVDGEWCGDGSTRQYAPLSPAIHLGAERLLILDTHYHKPRSVAAGARP
                     ATYPTLSRIGGYLLDTVFSDSLSADLERAERVNRMLAQIPAGAIPLGAPSFRPLETLI
                     IAPSRGPGAIAAHHIGTLPKGIRWMLRSFGGEKEGGDLLLSFMLFEADYCRELIELGR
                     HDARSRADELRRFLGIRSTVVVSAPANLHPPAASDTLQARRAP"
BASE COUNT          493 a         1112 c         1134 g          651 t
ORIGIN      
        1 atgtcggcgc atgcccgcac gctgctcgcg ggggaggcgg tgctggtccc ggtgcgcgac
       61 gtgaaactcg gcctcggccg ctggcaggga atcttcctct gggaacaccg ccgcggcgcg
      121 catcggcgcg aagtcctgat caccgtaatc ggcgactagc cccagcgccg gcgatcgaga
      181 tgcctcagtg cgttcccgct gccggcggcg gcgcggccgg cggaccgaat tcgaaatacg
      241 gcgggctcgg cttcttgccc ggcgggagcc aggcgggagc cggccctccg gcggggccga
      301 gagcgcgcag atagcggtag atgctcgcga ggtcgtaatt gctcatgctc gcgaagacgt
      361 accagggcat cggcggcctc gccttcgtgg tccgcaattg ttcgatccac tgcttttcgt
      421 cgaggctcgc ggcgagcagg cgcagattca ccgcataggt cgtgccccag gggccgagcc
      481 agccgatgcg ctcgccggtg agccagagac gctccggcac ctgcccggcc tcattgccgt
      541 agcccggcgt gtggcagtcg ttgcagcccg tgatcatgac gagatggcgg ccatgcgcga
      601 ccatcttctg attcgccgca ctcgtcgccg ccgcgaacgc cagcggagcc agagccagga
      661 tcatcaccgc ggtgatgaac cgatgcaatc gcgcaagcat gacgcctctc cgcacttgcc
      721 gtcgcaatgc taccatgccg gcgctgcatc cccgccgatc catggccccc gacgaaaccc
      781 tcccgcgaga ttccgccgcc gcctccgact tctgggggca tccgcggctt ctgtggatgc
      841 ttctcgccgt cacggtcggg ctcaatttcg ccttctacgg gtttcgcgcg tttctcgcgc
      901 cctacgcggc cgaggctttc ttctccggcc tgccgcaggc cgaggccctg aagcaggcga
      961 atttcctgtt cgccggcttc ggcgctctta cctacgcgac ggcaattctc ggcggctggg
     1021 tcgccgacaa catgctcggc gaggtgcagg cgctgcggat ttcgctgtgg ctggcaacgc
     1081 tcggcattct cgccctggcc tggccgaccc gggaaggttt cacgctggcg ctcgcgtttt
     1141 tcgtgctggc ggcgggtctc gggattccgc tgaccgttct catcgggcgc aattacggca
     1201 agggcgattc gcgtcgcgat gccggttaca ccttgttcta tctcgcgatc aatctcggcg
     1261 gcttcatcgc gcccttcatc tgcgcggcct ggatcggcgt ccattacggc tatcgctggg
     1321 gcttcattgc ggcggcctgc ggcatggcgt ttggcgcgct cgtcttcgag ttgcgtcacc
     1381 gacgcatgtc cggagcacgg gagaaggtac gcttccagcg gcgctggtcg gcgccggccg
     1441 taattgtcgc gatgctggtg ctgctgtggc cctgtgcctg gctgcttgcc catccgacgg
     1501 aaatgaccat cgtcgtgtat gcgttgatgg gcttgctcgt gttgtatttc gtcgggagca
     1561 gcctgcgccg ccgcgatcgc gtgcaggccc atcgttatct cgccatgctg attctctttg
     1621 tcgccatggt gctcttctgg gcctggagtc tcgagagcgc gagcgcgctc aactttttcg
     1681 cccgcgacta cgtggacgcg cccttcaact tcacactgtt ccagtcggta aacccgcttt
     1741 acatcctgat tctcgcggta ccgctctcgc tgctgtggcc gtggctggat cggcgcggca
     1801 tcaatccctc gactccgcgc aagttcggca tcggtctcct tctggtcgcg ctcggctacg
     1861 gcgtgctgct cgccgcaacc cattttctgg ttggcgccga cggacacatc gactggtggc
     1921 cgctgatgct ctggtatctc tttgcgacgc tcggcgaact cgcgctctcc ccgatcggct
     1981 acgcgatgat cgggaacctc gccgcctccg acgaagcgtc gatcgcgatg ggaggctggt
     2041 tcttcggcgt gagtgtctcc tatgatctcg ccgggcagat cgccgcgatg acgaccagcg
     2101 gcggcatcgc cggctattcg caggtgttcg cggggctctt cgtggtcggc gttgcaatcg
     2161 ccctgctctt tctcgtcgcg gctccctgga tcgtcaaact catgcacgga gtgcgctgag
     2221 cgcgatcggc ttcgacggcg gtcgctcctt cggcctcagg gcgcgcggcg ggcctgcaat
     2281 gtatcgcttg ccgccggcgg gtgcaggttg gccggagcgg aaacgacgac ggtgctgcgg
     2341 atcccgagga agcggcgcag ttcgtccgcc cggctgcggg catcgtggcg gccgagttcg
     2401 atcagctcgc ggcagtaatc cgcttcgaac agcatgaagc tcaggagaag atcgcctcct
     2461 tccttctcgc cgccgaagga gcgcagcatc cagcgaattc ctttgggaag cgtgccgata
     2521 tggtgcgccg cgatcgcacc cggtccgcgc gagggcgcga tgatcagcgt ttccagcgga
     2581 cggaagctcg gagcgccgag cggaatcgct cccgcgggga tctgcgcgag catgcgattc
     2641 acccgctccg cgcgttcgag atccgcactc aggctgtcgg agaaaacggt gtcgagaaga
     2701 tagccgccga tgcgcgagag cgtcggatag gtcgccgggc gcgcgccggc cgcgacactt
     2761 cgcggcttgt gatagtgcgt gtcgagaatg agcaggcgtt ccgctcccag atgaatcgcg
     2821 ggcgagagcg gggcgtactg gcgcgtggag ccgtctccgc accattcgcc gtcgacccgc
     2881 accgccggaa agacgatcgg cagcgcgagc gaggccatga tgtgatcgag tgtcatcatg
     2941 acggggcgtc cctcgcgccg ttcgcgccac caaggctcga cgtcgggtgc ggcctgaaaa
     3001 taggtgagcg agcgctcgga gctgtagccc gcgatcgtga tcgtcagtcc gcgcagcaaa
     3061 cccgcgtcga tgttgcgctg gatcgcggag aactcgagat tctcatcgag cagtttgcgc
     3121 agcggcccgt tgtcgagcag agagcgcggg ttgctctcac ccgcgccgcc ggagacgaag
     3181 gcccacagcc agcgggcgac gaccgcaaag atcttgcggt aatcggaact gtagacgtcc
     3241 ttgacgcgca gtgccgacca gagcgcggcg agcgcggcgc tcgccttgcg gtaatccggc
     3301 atttgcgtgg cgagatacgc ggcgctcatg gcgccggcgg aggttcccgc gatgatgtcg
     3361 aagggaaccg gatcgccgct cgcgtagagt
//
LOCUS       DASUJG010000254         2219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1054180, whole genome shotgun sequence.
ACCESSION   DASUJG010000254 DASUJG010000000
VERSION     DASUJG010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054180"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..1036)
                     /gene="trxB"
                     /locus_tag="VFX38_05465"
     CDS             complement(71..1036)
                     /gene="trxB"
                     /locus_tag="VFX38_05465"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014777466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX5314337.1"
                     /translation="MAEEKHCRLLILGSGPAGYTAAVYGARANLAPVLITGIAQGGQL
                     MTTTDVDNWPGDVEGLQGPDLMTRMFAHAERFGTEIIFDHVNAAELGESPLRLVGDNA
                     TYTCDALIIATGADARYLGLPSEEMYKGKGVSACATCDGFFFKDQEVAVVGGGNTAAE
                     EALYLANIAARTTLIHRREALRAEKMLQQRLFEREREGRLQILWNRVVEEVLGDGSAV
                     TGVRLRDAGASGAAQDLAVAGVFIAIGHTPNTAIFEGQLEMQNGYIVVKGGRHGDATA
                     TGVPGVFAAGDVMDHVYRQAITSAGSGCMAALDAEKYLEARGLGG"
     gene            1206..>2219
                     /locus_tag="VFX38_05470"
     CDS             1206..>2219
                     /locus_tag="VFX38_05470"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX5314338.1"
                     /translation="MAQARKVRRETEDSVALWLTAALREALFWLTAALAVVLAVALAS
                     FHPGDPSFTSTGTGAVRNLIGPAGAWTSDVLLLVLGLSAYILPVLLAYCGWMIFRGLA
                     RHGMLVPLLRIAAFLLALLALSTLLTLLASPGPGWLPGGIGGILGYVIGTPVTKIVGE
                     TGAGLLLLAVLLAGVTLATGLSWIALMDRLGSGLLRVYGWVSLRGRHWQEGRAARADR
                     SERVAIVEREVRRRKSRPQPRIEPSIAALEPGPRAQRERQRSLFISPETTGGLPPLEL
                     LDPPPARAGGYSEDALAAMSRQVELKLRDFGVEVEVVAVYAGPVITRFELQPAAGVKV
                     SQIS"
BASE COUNT          306 a          771 c          734 g          408 t
ORIGIN      
        1 ccgcgagcgc gttccagacc ttcgcgggga tctccgtgat cgaggtgtgg atccgctctt
       61 tcgtaagccc ttacccgccg aggccgcggg cctcgaggta tttctcggcg tcgagtgcgg
      121 ccatgcagcc gctgccggcg ctggtgatcg cctgacggta cacgtgatcc atcacgtcgc
      181 ccgcggcaaa aacgcccggc acgccggtcg cggtagcgtc tccgtggcgt ccgcctttca
      241 ccacgatgta gccgttctgc atctcgagct ggccctcgaa gatcgccgtg ttcggcgtgt
      301 ggccgatggc gatgaagact ccggcgaccg cgagatcctg cgccgcgccg gaggccccgg
      361 catcccgcag ccgcactccg gtcacggcgc tgccgtcgcc gagcacctcc tcgacgacac
      421 ggttccacaa gatttgcagc cgcccctcgc gctcgcgctc gaagagccgc tgttgcagca
      481 tcttttccgc gcgcagcgcc tcgcgccgat ggatcagggt ggtgcgcgcg gcgatgttcg
      541 cgagatagag cgcctcttcc gccgcggtgt tgccgccgcc gacgaccgcg acctcttgat
      601 ccttgaagaa aaatccgtcg caggtcgcgc aggcggaaac tcccttgccc ttgtacattt
      661 cctccgaggg aagaccgaga tagcgggcgt cggctccggt tgcgatgatg agcgcatcac
      721 aagtgtaggt cgcgttgtcg ccgaccagac gcaagggact ttcaccgagt tcggccgcgt
      781 tgacgtggtc gaagataatc tccgtgccga atctctcggc gtgcgcgaac atgcgggtca
      841 tgagatccgg cccctgcagg ccttcgacgt cgccgggcca gttgtccacg tcggtcgtcg
      901 tcatgagttg tccgccctgc gcaatcccgg tgatcagcac cggcgcgaga ttggcgcgcg
      961 cgccatagac ggccgcggtg tagcccgccg gtccggagcc gagaatgagc agccggcaat
     1021 gtttctcttc cgccattttt cttcccgtgg aatggaacgc agacgaccct cgccttcgat
     1081 cgccgccgcg ctcacagtgt agcgaatgcc tgcattagcc ggcggccgcg ccgcctcggg
     1141 gcttgggcgc ggcgcgcggg cgcgctacac tttgcagcgt tgaggacggc gagcagcctc
     1201 gtttcatggc ccaggcaaga aaagtccggc gcgaaaccga agattccgtg gcgctgtggc
     1261 tcacggccgc gttgcgcgag gcactgttct ggctgaccgc cgcgctcgcg gtggtgctcg
     1321 cggtcgcgct cgcgagcttt catcccggcg acccgagctt caccagcacg ggaacgggag
     1381 ccgtgcgcaa tctcatcggg ccggcgggcg cctggaccag cgacgtgctt ctgctggtgc
     1441 tcggcctgag cgcctacatc ctcccggttc ttctcgccta ctgcggctgg atgattttcc
     1501 gcggtctcgc gcgtcacggc atgctcgtgc cgctcctgcg catcgccgca ttcctgctcg
     1561 cgttactcgc gctttcgact ctgctgaccc tgctcgcctc tccggggccg ggatggcttc
     1621 ccggcggcat cgggggcatt ctcggctatg tcatcggcac gccggtcacg aagatcgtcg
     1681 gcgaaaccgg cgccggactg ttgctgcttg ccgtactgct cgccggggtg acgctcgcga
     1741 ccggcctttc ctggatcgcg ctcatggatc gcctcgggag cggtttgctg cgcgtctacg
     1801 gctgggtctc gcttcgcggc aggcactggc aggaagggcg cgcggcgcgc gccgatcgca
     1861 gcgaacgcgt cgcgatcgtc gagcgcgaag ttcgccgtcg caagagccgg ccccagccgc
     1921 gcatcgagcc ttcgatcgcg gcgctcgagc ccggcccgcg cgcgcaacgt gaacgccaac
     1981 ggtcgctgtt catttctccc gagaccaccg gtggcttgcc gccgctggaa cttctcgatc
     2041 cgccgcccgc gcgtgccggc ggctacagcg aggacgcgct cgccgcgatg tcgcggcagg
     2101 tggagctgaa gctgcgggac ttcggcgtcg aagtcgaggt cgtcgccgtg tacgccgggc
     2161 ccgtcatcac ccgcttcgag ttgcagcccg ccgcgggagt caaggtcagc cagatttcg
//
LOCUS       DASUJG010000255         2414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1666087, whole genome shotgun sequence.
ACCESSION   DASUJG010000255 DASUJG010000000
VERSION     DASUJG010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1666087"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..717
                     /locus_tag="VFX38_05475"
     CDS             142..717
                     /locus_tag="VFX38_05475"
                     /inference="COORDINATES: protein motif:HMM:NF019081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HEX5314339.1"
                     /translation="MKRRHCMMWVAALAFAPAWALAEGSGLDFNVNAHAVRAAYDSEV
                     TLSGLDFSIEALHNSDNGNVADVGLGLRSNANPGASPVTALIGVKALWVDPSYSGVSA
                     GYGLALGGGVQFSLPSYNRIAFGAALYWAPSVTSFSNLDHFIEEEARAGYRVLPNGTV
                     YLGYRHMTAAFTNTDDLVIDNGFNVGMALRF"
     gene            730..1473
                     /locus_tag="VFX38_05480"
     CDS             730..1473
                     /locus_tag="VFX38_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018168501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX5314340.1"
                     /translation="MSGHSKWANIQHRKNAQDARRGRVFTRLIREVTTAARIGGADPA
                     TNPRLRLAFERANAANVPKDNLERALKRASGDADGQTIEEVGYEGYAPGGVAVLVECM
                     TDNRNRTVADVRHAFSRHGGNLGADGSVAYLFNRVGSIAVGAQANEERLLEAALEAGA
                     EDVAHDDDGGFEVLTDPNDFEAVRDRLRAGGFALAEAEITMRPASRVELDAPTAARVL
                     ALVDTLDELEDTQQVYTNARFPAEALAER"
     gene            1491..1991
                     /gene="ruvC"
                     /locus_tag="VFX38_05485"
     CDS             1491..1991
                     /gene="ruvC"
                     /locus_tag="VFX38_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX5314341.1"
                     /translation="MAPLVILGIDPGSRITGYGLIAVEGNERSVLDYGVVRAPPVELG
                     RRIRAIYEGIAAIVARHVPDEIAIEQAFVHLNPAVALALGQARGAALAAALASERPLA
                     EYAPRSVKLAIVGSGKAEKIQVAHMIRHLLRIAGPIPEDASDALAIALCHAHSRGLAA
                     RMAARA"
     gene            1988..>2414
                     /gene="ruvA"
                     /locus_tag="VFX38_05490"
     CDS             1988..>2414
                     /gene="ruvA"
                     /locus_tag="VFX38_05490"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007143906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX5314342.1"
                     /translation="MIGYLRGRLAERRPPRLVVEVGGVGYELEAPLSTFAILPAGAAE
                     VKLFTHLMIREDTQQLFGFASEEERSLFRALLRASGVGAKLALAILSGMSVEEFARTV
                     AERDIATLRRLPGIGQKTAERLVVEMADRIEGIRGPRAGG"
BASE COUNT          391 a          807 c          828 g          388 t
ORIGIN      
        1 agtggctcga gtattgtctc gattcccggc gcaaggcgaa ttgccgcacg tcggcgctcg
       61 cgcgggcacc ctgacggtcg tcgcgcgccg cctcgcagtc gtggcatcat atagcctgat
      121 cgggcaggca cggatcgcga aatgaaacga cgccattgca tgatgtgggt cgccgccctt
      181 gcatttgcgc cggcttgggc actcgccgag ggcagcggtc tcgatttcaa cgtcaacgca
      241 catgctgtgc gcgcggccta cgattccgaa gtcacgctct cggggctcga tttttccatc
      301 gaagcgctgc acaattccga caacggaaac gttgcggacg tcggcctcgg actgcgctcg
      361 aacgcgaacc cgggagcctc gccggttacg gctctcatcg gcgtgaaggc gctgtgggtc
      421 gacccgagct actcgggggt gtcggccggc tacgggctcg cgctcggcgg cggagtgcag
      481 ttctcgctcc cgagctacaa ccgcatcgcc ttcggcgccg cgctctactg ggcgccttcg
      541 gttacgagtt tcagcaacct cgatcacttc atcgaggaag aggcgcgggc gggctaccgg
      601 gtgctgccga acgggacggt ctatctcggc taccggcaca tgaccgcggc cttcaccaat
      661 accgacgacc tcgtgatcga caacgggttc aacgtgggca tggcgctgcg cttctgagcg
      721 ggagtcgcga tgtccgggca cagcaagtgg gcgaacatcc agcatcgcaa gaacgcgcag
      781 gacgcgcgcc gcgggcgcgt tttcacacgc ctgattcgtg aggtcacgac ggccgcacgc
      841 atcggcggcg ccgatcccgc aaccaatccg cgactgcggc tcgccttcga gcgggcgaat
      901 gccgccaacg tcccgaagga caacctcgag cgcgcactca agcgcgcgag cggggacgcg
      961 gacggacaga cgatcgagga ggtaggctac gaaggctacg cgccgggcgg ggtcgccgtg
     1021 ctcgtcgaat gcatgaccga caatcgcaat cgcaccgtcg ccgacgtgcg ccacgccttc
     1081 agccgccacg gcggcaatct cggcgccgac ggctcggtcg cgtatctgtt caatcgcgtc
     1141 ggctccatcg ccgtcggtgc gcaagcgaac gaggagcgtc tgctcgaagc cgcgctcgaa
     1201 gcgggcgcgg aagacgtcgc gcacgatgac gacggtggct tcgaggtgct taccgatccc
     1261 aacgatttcg aggcggttcg cgatcggctg cgcgcgggcg gcttcgcact ggcggaggcc
     1321 gaaatcacga tgcgtccggc gagccgggtg gaactggatg cgccgaccgc cgcgcgggtg
     1381 ctggcgctcg tcgatacgct cgacgaactc gaggacacgc agcaggtgta caccaacgcc
     1441 cgctttcccg ccgaggcgct tgcggagcgc tgaggcagat cgaacgacgc gtggcgccgc
     1501 ttgtcattct ggggatcgat ccgggttcgc ggattaccgg ctacggattg atcgctgtgg
     1561 aggggaatga acgttccgtt ctcgattacg gggtggtgcg cgcaccgccc gtcgaactcg
     1621 gccggcgcat ccgtgcaatc tacgaaggga tcgccgcgat cgtcgcccgc catgtgcccg
     1681 atgaaatcgc aatcgagcag gccttcgtgc atttgaaccc ggcggttgcg ctcgccctcg
     1741 gccaggcgcg cggagcggcg ctcgccgccg cgctcgcgag cgaacggccg ctcgccgagt
     1801 acgcgccgcg gtcggtcaaa ctcgcgatcg tcggctccgg caaggcggaa aagatccagg
     1861 tcgcgcacat gatccgccat ctgctgcgca tcgccgggcc gattcccgaa gatgcgagcg
     1921 acgcgctcgc gatcgcgctt tgccatgcgc acagccgcgg cctcgccgca cgcatggcgg
     1981 cgcgcgcatg atcgggtatc tgcgcgggcg tctcgccgag cgccgcccgc cgcgtctcgt
     2041 ggtggaggtg ggcggtgtcg gctacgaact cgaggcgccg ctttcgacct tcgcgatcct
     2101 gcccgcgggc gcggcggagg tcaagctctt tacgcacctg atgattcgcg aagacacgca
     2161 gcagctcttc ggcttcgcga gcgaggagga acgcagcctc tttcgcgcgc tgctgcgcgc
     2221 gagcggagtc ggagccaagc tcgcgctcgc gatcctttcc ggcatgtcgg tcgaggagtt
     2281 cgcgcgcacg gtcgccgagc gcgacatcgc gaccctccgg cgccttcccg gcatcgggca
     2341 gaagaccgcc gagcgcctgg tcgtcgaaat ggccgatcgc atcgaaggaa ttcgcggccc
     2401 gcgagccgga ggcg
//
LOCUS       DASUJG010000256         2143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_933761, whole genome shotgun sequence.
ACCESSION   DASUJG010000256 DASUJG010000000
VERSION     DASUJG010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933761"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VFX38_05495"
     CDS             <1..606
                     /locus_tag="VFX38_05495"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX5314343.1"
                     /translation="RAFTLLEPRPARSVLFLATTSEEQGLLGAQYYADHPAIPLAHTV
                     ADINMDIMNVYGRSRDLMVIGWDKSGLQPLLASAAAKEGLRLEPYAHPETGMFYRTDL
                     LEFARHGVPAIVTASGHDYVGRPAGWGDRQWAEYFADRYHKPADEFDPDWDLSGLVQQ
                     AQALFLLGAGLAEGDAWPDWNEGVPFKALRDRERPPAAASH"
     gene            complement(623..1327)
                     /locus_tag="VFX38_05500"
     CDS             complement(623..1327)
                     /locus_tag="VFX38_05500"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5314344.1"
                     /translation="MKMRLPMQFGAAAMVALTACAAAAQPPAAATAPVAASAASIARG
                     KILVTTCAFCHGMEDYTVPYPTQHVPLIGGQHEAYALSALDEYAKGKRHFATMHAQAA
                     SLTQGQIEDIAAYIASVAPTGVTANTGPTYGAPSVGSIAAAAVFSRAPVAAASCVACH
                     GKHGVSTNPEFPTLAGQYQDYLLQALKEYKTGERKNAIMNGVAASLSLADMKQLAAYF
                     AKQHGPLKLLPKAGPD"
     gene            complement(1446..2081)
                     /locus_tag="VFX38_05505"
     CDS             complement(1446..2081)
                     /locus_tag="VFX38_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX5314345.1"
                     /translation="MRFTVEGLVDGDWLPDQFAFGVAHASERMRLGPNRNPGVHWSGE
                     PAGTRTLVLLCYDDDVPAEAEDANREDRTIAHSARRTRFYHWILVDLAPGAECIGEGS
                     VSNGVTPRGKKRREGPMGARQGLNDFTDFLRGDPELEGKYYGYDGPCPPWNDERLHRY
                     HFVLHATDLDRCPVEGDFTGSEVEAALAGHVLASARVSGHYSLFPPHRERR"
BASE COUNT          357 a          710 c          709 g          367 t
ORIGIN      
        1 cgcgcgttca cgctcctcga gccgcggccg gcgcgctcgg tgctgttcct cgccaccacc
       61 tcggaggagc agggactgct cggcgcccag tactacgccg accatccggc gatcccgctc
      121 gcgcacaccg tggccgacat caacatggac atcatgaacg tgtacggccg cagccgcgat
      181 ctgatggtca tcggctggga caagtcgggc ttgcagccgc tgctcgcgag cgcggcggcc
      241 aaggaaggcc tgcgcctcga accctacgcg cacccggaaa cgggcatgtt ctatcgcacg
      301 gatctcctgg aattcgcgcg ccacggcgtg ccggccatcg tcaccgcgag cgggcacgat
      361 tatgtcggcc gtccggcggg ctggggcgat cgccaatggg cggagtactt cgccgatcgc
      421 tatcacaagc cggccgacga gttcgatccc gactgggacc tttccggact cgtgcagcag
      481 gcgcaggcgc tctttctctt gggcgccgga ctcgccgagg gcgatgcctg gccggattgg
      541 aacgaggggg tccccttcaa ggcgctgcgc gaccgcgagc gcccgcccgc cgcggcctct
      601 cactgagcgg agagtccgcg cttcagtccg ggccggcctt cggcagcagc ttcagcggtc
      661 cgtgctgctt ggcgaaatag gcggcgagtt gcttcatgtc ggcgagtgaa agcgaggccg
      721 cgacgccatt catgatcgcg ttcttgcgct cgcccgtctt gtattccttg agtgcctgca
      781 gcagataatc ctgatattgc cccgcgaggg tcgggaactc gggattggtg ctcacgccgt
      841 gcttgccatg acaggcgaca cagctcgccg ccgcgaccgg tgcgcgggaa aaaaccgcgg
      901 ccgccgcgat cgatccgacg ctgggcgcac catacgtggg accggtattt gcggtaacgc
      961 cggttggagc gacactcgcg atgtaagccg cgatgtcttc gatctgccct tgggtgagtg
     1021 acgccgcctg cgcatgcatg gtggcgaaat gacgcttgcc cttcgcgtat tcgtcgagcg
     1081 cggagagagc gtaggcctcg tgctggccgc cgatgagcgg cacatgctgc gtcggatacg
     1141 gcacggtgta gtcttccatg ccgtggcaga aggcgcaggt cgtgaccagg atcttgccgc
     1201 gtgcgatcga agcggcgctc gccgcgacgg gtgccgtcgc ggctgcggga ggctgagcgg
     1261 ccgccgcgca ggccgtgagc gcgaccatcg cggcagcgcc gaattgcatc ggcagacgca
     1321 tcttcatccg cccttcgctc gtccgtgcat ggcagcggca attgtaacgg atgcgccggc
     1381 agcgcgaacg gctcggcggg cgttggtttc gccacgacca tccgacggca tccgcccgga
     1441 tgatttcagc ggcgctcgcg atgcggcggg aacagactgt agtgcccgct cacgcgggcg
     1501 gaagcgagca catgtcccgc gagtgcggcc tcgacctcgg agccggtgaa gtctccctct
     1561 accggacagc gatcgagatc ggtcgcatgc aaaacgaaat gataccgatg gaggcgctcg
     1621 tcgttccacg gcggacaggg cccgtcatag ccgtaatatt tgccttcgag ttcgggatcc
     1681 ccgcgcaaaa aatccgtgaa atcgttcagc ccctggcgcg cacccatcgg tccctcgcgc
     1741 cgcttcttgc cgcgaggagt cacgccgttg gaaacgctgc cttcgccgat acattcggct
     1801 cccggagcaa ggtccaccag aatccagtga taaaagcgtg tgcgccgagc gctgtgggcg
     1861 atggtccggt cttcgcgatt ggcgtcttcg gcctcggccg gtacgtcgtc gtcatagcag
     1921 agcagcacga gcgtgcgcgt tcccgccggc tcgcccgacc agtgcacgcc cgggttgcga
     1981 ttggggccga gacgcatgcg ttcggaagca tgcgccacac cgaaggcgaa ctgatcggga
     2041 agccagtcgc cgtccacaag tccctcgaca gtgaaacgca tgagcacgct cccgacaatc
     2101 ctttaccatc atacaaccgc ggtcgccgcc gtttgcagga acc
//
LOCUS       DASUJG010000257         5639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_443829, whole genome shotgun sequence.
ACCESSION   DASUJG010000257 DASUJG010000000
VERSION     DASUJG010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5639
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443829"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..912)
                     /locus_tag="VFX38_05510"
     CDS             complement(76..912)
                     /locus_tag="VFX38_05510"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX5314346.1"
                     /translation="MTPLAWIAGTVMPLAEARIALDDPGFLLGDGVFETLRARKGKPF
                     RWDLHAERLAAGLAVVGIAAEALAVAEGAVHELATRAAHDCDDDVYLRVQVSRLAASR
                     AEVTALVRPLPAFPEHLYRRGARLAIADWHRDPADPLAGVKSLSYLPQVWARRAVQAR
                     GFDDALILNSFGRVCEAAHANVIARHGRVLYAPGPEEGALDGVTRRVLLAALPEDDYA
                     VETRLDRIALVEAEELVLISTLAGVVPVSGIAGVDRLYAGAAGVLAMHLAHAYAVLLD
                     SA"
     gene            complement(909..3008)
                     /locus_tag="VFX38_05515"
     CDS             complement(909..3008)
                     /locus_tag="VFX38_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEX5314347.1"
                     /translation="MVAGRSGDLLCRGLRRVALARLPQPSQALEALLPLGAALLESRT
                     DHPGARYSVVAFSPLAELRLDRGRLTLRLPGGGEEEIAGPPLPALFARLEAVRTMSVA
                     ADVPFAGGWIGYFAHELGCLMMEASPAAAMPPARPTGPLAVFRLYRDALVVDHRAQTA
                     ALYASDWGEGEEDVQRRLAAAQGLLHREPKTSIAPEVVDPPALTLAPREFAERQEKLQ
                     VLIRAGDCFQANLTASWAWPWRNGAPTVAELVALYRRYTEANPGAWCGLFPDDEATIL
                     CGSPELLVDWRHQRVAMRPIAGTRPRGADSARDAAFAAELRGDAKERAEHAMLVDLAR
                     NDLARISRPGSVRVENFAGVERYRHVMHLVSEVTGEARDGLDLESLLRAVFPGGTVTG
                     APKRRALSRIGELERGPRGAYTGALGYVALGGASQWNLMIRTLTATPTHLVAHAGCGI
                     VAGSQSELEAAELAAKARAQAEAALGRATPATPADRCGEVEPGSRWESARGVPIARPA
                     RVLIVDFEDSFVHNLGDYCRRLGAETAVVSAHASPGPAWESTVTHLILSPGPGRPEDF
                     PLWRHHLEFAEARGIPVLGVCLGHQALAEAAGAKLVCHSETVHGSASRLLRLPASRTD
                     PLFGAWSGETVGRYHSLVVIESVPAMEALATLEDGTLMAVRYRARPHWGLQFHPESLL
                     TENGLDLISAFLHVESP"
     gene            3038..3733
                     /locus_tag="VFX38_05520"
     CDS             3038..3733
                     /locus_tag="VFX38_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEX5314348.1"
                     /translation="MIHAYSWATPNGHKLHIMLEETGLAYTLHPVDIGAGGQFEPEFL
                     KISPNNKIPAIVDDDGPGGGPFALAESGAILIYLADKSGRFFPTEPRAHYATLQWLMF
                     QMGHIGPMLGQAHHYRSYAPIHISYGIERYTREARRLYGVLDRQLSENEFVAGREYTI
                     ADIAIFPWLHSHLDHGVEVAEYPAVSRWFDLVAARPAVARGLAVLAGERSASTDDKER
                     FENLFGVRQFARR"
     gene            complement(3772..>5639)
                     /locus_tag="VFX38_05525"
     CDS             complement(3772..>5639)
                     /locus_tag="VFX38_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX5314349.1"
                     /translation="ITYRTRSAVRDLGKALGLDALEVERLARAFQWWDGGTLDAERIR
                     EAGLDPASPVLARLAALLRQLIGFPRHLSQHVGGFVISRGPLAELVPVENAAMPQRTV
                     IQWDKDDLDALGLLKVDVLALGMLSALRRALELRNRLRGGALTLAAIPAEDPAVYAML
                     RQADTVGVFQVESRAQMAMLPRLKPATFYDLVIQVAIVRPGPIQGEMVHPYLARRAGT
                     EAVSWPSAEIKEVLGRTLGVPIFQEQVMRLAVVAAGFTPGEADQLRRAMGAWRRTGSL
                     GPFEDKLLHGLRERGYPEDFARRLYQQIRGFGEYGFPESHAASFALLAYASAWLKHHE
                     PAAFVCALLNSQPMGFYAPAQLVADARRHGVEVRPVVITASGWDCSLEVRKAQTQPAL
                     RLGLCLVRRLSQEGAKRVVAARAERAFDSVADLARRARLDRRDLDALGNADALKPLAG
                     HRYRSAWKTQGIAPDMALGATSFAEAEPLLAAPGEGENLVADYASIGLTLGRHPLALL
                     RERLDREGIATAAALLGARNGGRTRTVGIVTHRQRPGTGQIVFVTLEDESGTINVIVR
                     PAVAERDRRVLLDARLLGAAGVIQREAGVLHVIARRLFDYTHLLGTLAARSRDFR"
BASE COUNT         1011 a         1956 c         1825 g          847 t
ORIGIN      
        1 gcccggcaga ttataggcgt gttgcggccg acggaacgtt catacgaccg gcaagccaga
       61 cggcctcccg cttttttatg cggaatcaag gagtaccgcg tacgcatgcg cgagatgcat
      121 agcgagaacc ccggccgccc cggcgtaaag ccgatcgacg ccagcgatcc ccgataccgg
      181 aacgaccccc gcaagggtac tgatcagcac gagttcttcc gcttcgacga gtgcaatccg
      241 gtcgagacgg gtttcgacgg cgtaatcgtc ttcgggcaac gccgcgagca acacgcgccg
      301 ggtcacgcca tcgagcgccc cctcctccgg tccgggtgcg tagagtacgc gcccgtggcg
      361 cgcgatgacg ttggcatgcg cggcttcgca tacgcgcccg aaagaattca atatcagcgc
      421 gtcgtcaaaa ccccgtgcct gcacagcacg ccgcgcccag acctgcggca aatagctcaa
      481 cgatttcacg cccgcgagcg ggtcggcggg atcgcggtgc cagtcggcaa tcgcgagtct
      541 cgcgcccctg cggtacaggt gctccggaaa agcgggcagc gggcgcacga gtgcggttac
      601 ctccgccctg cttgccgcga gacgcgaaac ctgtacgcgc agatacacgt cgtcgtcgca
      661 atcgtgcgcg gcgcgggtcg cgagttcgtg cactgcgcct tcggcgacgg cgagtgcctc
      721 cgcggcgatg ccgacgacgg cgaggccggc ggcaagccgt tcggcgtgca aatcccaacg
      781 gaacggtttc cccttgcgcg cgcgcaaggt ttcgaatacg ccgtcaccga gaagaaaacc
      841 cggatcgtcg agtgcgatcc gggcctcggc aaggggcatt acggtgcccg cgatccacgc
      901 cagcggagtc acggtgattc gacgtgcaaa aaggccgaaa tcagatcgag accgttctcg
      961 gtcagcagcg actcgggatg gaattgcaag ccccaatgcg gccgggcacg gtagcgcacg
     1021 gccatcaagg tgccgtcctc caaagtcgcg agcgcttcca tcgcgggaac cgattcgatc
     1081 accacgagcg aatgatagcg gccgaccgtt tctccgctcc atgcgccgaa cagcggatcg
     1141 gtgcgcgacg ccggcaaacg caggagccga cttgcgcttc cgtgcacggt ttcggagtga
     1201 caaacgagct tcgcccccgc cgcttccgcg agcgcctgat gcccgagaca gacgccgagc
     1261 accggaatgc cgcgcgcttc ggcgaattcc aggtggtgcc gccacaacgg aaaatcctcg
     1321 ggccggccgg gacccggcga gagaatcagg tgcgtgaccg tgctttccca agcgggcccg
     1381 ggcgacgcgt gcgcgctcac gaccgccgtt tccgccccga ggcgccggca atagtcgccg
     1441 aggttgtgca cgaaggaatc ttcgaaatcc acgatcagca cgcgcgcggg gcgcgcgatc
     1501 ggaaccccgc gcgcggactc ccagcgcgaa cccggctcga cttcgccgca gcgatccgcg
     1561 ggagttgccg gcgtcgcccg cccgagcgcg gcctcggcct gcgcccgcgc cttggccgcg
     1621 agttccgccg cctcgagttc gctctggctg ccggcgacga tgccgcaacc ggcatgcgcg
     1681 acgagatgcg tcggggtcgc ggtcaacgtg cggatcatga ggttccactg gctcgcaccg
     1741 ccgagcgcga cgtagccgag cgcacccgta taagcgccgc gcggcccccg ttccaattcg
     1801 ccgatccgcg agagcgcgcg acgcttcggg gcgcccgtca cggtgccacc gggaaacacg
     1861 gcgcggagca gcgattcgag atcgagccca tcgcgggctt cgccggttac ttcgctcacc
     1921 agatgcatca catgacgata gcgttcgacg ccggcaaagt tctcgacacg caccgagccc
     1981 ggccggctga tgcgtgcgag atcgttgcgc gcgagatcca ccagcatcgc atgttcggct
     2041 cgttccttcg cgtcgccgcg aagttccgcg gcaaatgccg cgtcgcgcgc gctgtctgcg
     2101 ccgcgcgggc gggtgcccgc gatcggccgc atggcgacgc gctgatgccg ccaatccacc
     2161 agaagctcgg ggctgccgca gagaatcgtc gcttcgtcgt cggggaataa accgcaccag
     2221 gcgccgggat tggcttcggt atagcggcga tacagcgcca ccaattccgc gaccgtcggt
     2281 gccccgttgc gccagggcca ggcccagctc gcggtgagat ttgcctgaaa gcagtctccg
     2341 gcgcgtatca atacttgaag tttttcctgt cgttccgcga actctcgcgg agcgagcgtc
     2401 agcgcgggtg gatccacaac ctccggcgca atcgaagtct tcggctcccg atgcagcaat
     2461 ccttgcgcgg cagcgagacg ccgctgcacg tcttcctcgc cttcccccca atccgaagcg
     2521 taaagcgccg ccgtttgcgc acgatgatcg acgacgagcg catcacggta cagccggaac
     2581 accgcgagcg gtcccgtcgg gcgcgccggc ggcatggcgg ccgctggcga tgcttccatc
     2641 atgaggcaac ccaactcgtg tgcgaagtat ccgatccagc cgcccgcaaa gggcacatcg
     2701 gcagcaacgg acatagtgcg aacggcttcg agccgggcaa aaagcgccgg aagcggcggc
     2761 ccggcaattt cttcctctcc gccgccgggc aggcgaagcg tcaaacgacc acgatccagg
     2821 cgcagctccg caagcggcga aaaggcgaca acggaatagc gcgctccggg atgatcggtg
     2881 cgtgattcga ggagcgccgc acccagcgga agcaatgctt ccagcgcctg cgagggctgc
     2941 ggcaggcgcg cgagcgccac tcggcgcaac ccgcggcaaa gcagatcgcc cgaacgtccg
     3001 gctaccatgg ggaggtatcg acggcgagga cgaaacgatg attcacgcgt attcctgggc
     3061 gacgccgaac gggcacaagc tgcatatcat gctcgaagaa acgggacttg cctacacgct
     3121 gcatcccgtg gacatcggcg ccggcggtca gttcgaaccc gagtttctga aaatcagccc
     3181 caacaacaag attccggcga tcgtggacga cgacggtccc ggcggcgggc cgttcgcgct
     3241 cgccgaatcc ggcgccatcc tgatctatct cgccgacaag agcgggaggt tctttcccac
     3301 cgaaccgcgc gcgcattatg caacgcttca gtggctgatg ttccagatgg gtcacatcgg
     3361 gccgatgctg ggccaggcgc atcattaccg cagctacgca cccattcaca taagttacgg
     3421 catcgagcgc tacacgcggg aggcccggcg cctttacggc gtgctggatc gccaactctc
     3481 cgaaaacgaa ttcgtcgcag gaagggaata cacgatcgcc gacatcgcca ttttcccgtg
     3541 gctgcattcg cacctcgacc acggcgtcga agttgcggaa tacccggccg tgtcgcgctg
     3601 gttcgatctc gtcgccgcgc gccccgcggt tgcgcgcggc ctcgcggtgc tcgccggcga
     3661 acgctcggcc tccacggacg acaaggaacg cttcgagaat ctcttcggcg tccgccagtt
     3721 cgctcgccgc tgactcgtcg cggtcttgcc tccgcgaccg gccgcaggaa gtcagcgaaa
     3781 atcccgcgaa cgtgctgcta gcgttccgag cagatgcgta tagtcgaaaa gtcgccgggc
     3841 gatcacatgc agcacgccgg cctcgcgctg aatcacgccg gccgcgccga ggagccgcgc
     3901 atcgagcaag acccgccggt cgcgttcggc gacggccgga cggacgatca cgttgatggt
     3961 ccccgattcg tcttccaggg tcacgaaaac gatctgcccg gtgccgggcc gctggcgatg
     4021 cgtgacgatg cccacggtgc gggtgcgccc gccgttgcgc gccccgagca gcgccgcggc
     4081 ggtcgcgatc ccctcgcgat cgagccgttc gcgcagcagc gcgagcggat gccgcccgag
     4141 cgtgagaccg atgctcgcgt aatcggcgac gagattctcg ccttcgcccg gcgccgcaag
     4201 cagcggctcg gcctcggcga agctggttgc accgagcgcc atgtcgggag cgatgccctg
     4261 tgtcttccag gcgctgcgat aacggtgccc cgcgagcggc ttgagcgcat ccgcattccc
     4321 gagcgcatcg agatcgcggc gatcgagccg cgcgcgccgc gcaagatcgg cgacggaatc
     4381 gaaggcgcgc tcggcccgtg cggcgacgac gcgcttcgcg ccttcctgcg aaagcctccg
     4441 caccagacac aggccgagcc gcagggccgg ctgcgtttgc gccttgcgca cttcgagact
     4501 gcaatcccag ccgcttgccg tgatgacaac gggacgcacc tcgactccgt gacggcgcgc
     4561 atcggcgacc agttgcgccg gcgcataaaa ccccatcggc tgcgaattca ggagcgcgca
     4621 gacgaaggcc gccggttcgt gatgcttcag ccaggccgag gcataggcga gcagcgcaaa
     4681 gctcgccgca tgactttccg gaaatccgta ttcaccgaag ccccggatct gctggtaaag
     4741 ccgccgcgcg aaatcctccg gatagccgcg ttcgcgcagt ccgtgcagta acttgtcctc
     4801 gaagggtccg agcgaaccgg tgcgccgcca cgctcccatc gcgcggcgca gttgatcggc
     4861 ctcgccggga gtgaatccgg cggcgacgac ggcaaggcgc atcacctgtt cctgaaaaat
     4921 cggcacgccg agagtgcgcc cgagcacttc ctttatttcc gcgctcggcc agctcacggc
     4981 ttcggttccg gcacgccgcg cgagataggg gtgcaccatc tcgccctgga tcggtccggg
     5041 gcgcacgatc gcgacctgga tcacgagatc gtaaaaagta gcgggtttca ggcgcggaag
     5101 catcgccatc tgcgcccgcg attccacctg aaacacgccg accgtgtccg cctgccgcag
     5161 catcgcgtac accgcgggat cttccgccgg aatcgcggcg agcgtgagcg ccccgccgcg
     5221 caggcggttg cgaagttcga gcgcccggcg cagcgcgctc agcattccga gcgcgagcac
     5281 gtccaccttg agcagcccga gcgcatcgag atcgtccttg tcccattgga tcaccgtgcg
     5341 ctgcggcatc gcggcgtttt cgaccggcac cagttcggcg agcggcccgc ggctgatgac
     5401 gaaaccgccg acgtgctgcg aaagatgccg cggaaagccg atcagttggc gcaggagcgc
     5461 ggcgaggcgc gcgagcacgg gactcgcggg atcgagcccc gcctcgcgaa tgcgttccgc
     5521 atcgagcgtc ccgccgtccc accactgaaa ggcccgcgcc agacgttcga cttcgagcgc
     5581 atcgagcccg agcgccttgc cgagatcgcg caccgcgctt cgcgtccgat aggtgatca
//
LOCUS       DASUJG010000258         6415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1788399, whole genome shotgun sequence.
ACCESSION   DASUJG010000258 DASUJG010000000
VERSION     DASUJG010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6415
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1788399"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VFX38_05530"
     CDS             complement(<1..472)
                     /locus_tag="VFX38_05530"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX5314350.1"
                     /translation="MSETRTLPALVLDPATRALNAALAADPEALRTLRTLSGRRVAVE
                     ISDLSLVVPLAIAGERIELGGTPEGADATVSGRMSALIAAGRSGNPRGLTVAGDAEVV
                     QSLARVMARLPRAAWERVATVLGAGPSRTLERVAGALKKSLEGTRDRLNENVAEY"
     gene            complement(469..1209)
                     /locus_tag="VFX38_05535"
     CDS             complement(469..1209)
                     /locus_tag="VFX38_05535"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016210582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5314351.1"
                     /translation="MQENQTDFGYRRVSPTEKTRRVRAVFESVAPRYDLMNDLMSGGL
                     HRLWKRCALLQTGLRPGDRALDLAGGTGDLARGLARQVGPAGEVVLADINAAMIRRGR
                     ERLLDAGVGGVRCTVANAEALPFPSDHFGCITIGFGLRNVTHKELALAEMRRVLAPGG
                     RALVLEFSRPYEPLRRAYDLYSFTVLPRLGRWIAKDEASYRYLAESIRRHPAQEELRK
                     MMEATGFERVRYYNQAGGIVALHVGWKL"
     gene            complement(1345..1713)
                     /locus_tag="VFX38_05540"
     CDS             complement(1345..1713)
                     /locus_tag="VFX38_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEX5314352.1"
                     /translation="MSEGEAPRPIEIRGRRASRDLYLRFDDGAEFTLPFEYLRVFSPS
                     AEVTGHGGAPRLLVAGKENVGVSAVEPVGQYAVRIVFDDGHDSGLYSWRILRTLGENF
                     EHNWQDYRARLERAGIGRKG"
     gene            complement(1710..3059)
                     /gene="hslU"
                     /locus_tag="VFX38_05545"
     CDS             complement(1710..3059)
                     /gene="hslU"
                     /locus_tag="VFX38_05545"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEX5314353.1"
                     /translation="MTDTMTPREIEAELDRHIVGQTAAKRAVAVALRDRWRRMQLPAE
                     LRAEVMPKNILMIGPTGVGKTEIARRLARLAHAPFLKVEATKFTEVGYVGREVDSIIR
                     DLAEIAIKMARGEAKEAVRVRAEQAAEERLLDALLPRSHAVGFANEPRPPTEHDETRQ
                     KIRGQLRAGALDEREIEIEVSAAQPSMEILAPPGMEEMTGQLENMLRGMTGQRRENRR
                     VRVAEARRLLADDEAAKLVNEEEIRRDAIREVEENGIVFIDEIDKVARSARDGGVSGA
                     DVSREGVQRDLLPLVEGSTVNTRYGMVRTDHILFIASGAFHVAKPSDLIPELQGRLPI
                     RVELSALGVADFIRILTEPRAALTVQYVAMLATENVKLAFREDGVRRIAEIAHRVNEH
                     TENIGARRLQTVMQRLLETVSYEAPDRAGSSVEVDAAYVDAHLGDLADDEDLSRYIL"
     gene            complement(3056..3610)
                     /gene="hslV"
                     /locus_tag="VFX38_05550"
     CDS             complement(3056..3610)
                     /gene="hslV"
                     /locus_tag="VFX38_05550"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016352431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEX5314354.1"
                     /translation="MQQFHATTILCARRDGVVAMAGDGQVSMGETVLKANARKVRRLA
                     EGRVLAGFAGGTADAFTLAEKFESQLERSSGNLARAAVALAREWRSDRVLRRLEALLL
                     VADAETSLLISGNGDVIEPEEGVLAIGSGGPYALAAARALLRNSTLAAPAIARAALGI
                     AAEICVYTNPQITIETLPEEAASA"
     gene            complement(3822..4730)
                     /gene="xerC"
                     /locus_tag="VFX38_05555"
     CDS             complement(3822..4730)
                     /gene="xerC"
                     /locus_tag="VFX38_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEX5314355.1"
                     /translation="MSPDAEGFRRRFLEHLGFERRLSPHTIGGYGRDLAALAAWCDAN
                     GIGDWSALEPAGLSRYLAERHRDGLSARSLSRALAATRSFARHGLKRGGLERDFTIGV
                     RTPKLRRTLPATLDVDEAARLVEVDAGASFEAGRDRAILELFYSSGLRLAELAGLDLN
                     DLDLREGLVRVTGKGEKTRVVPVGSKAREALGDWLKARLAFAAPGEAALFLSRRGRRL
                     ARRSIEARISYWGRRQGLDRRVYPHLLRHAFATHVLESSGDLRAVQELLGHASLATTQ
                     VYTHLDFQHLARTYDQAHPRAHRKKP"
     gene            complement(4727..5422)
                     /locus_tag="VFX38_05560"
     CDS             complement(4727..5422)
                     /locus_tag="VFX38_05560"
                     /inference="COORDINATES: protein motif:HMM:NF016243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HEX5314356.1"
                     /translation="MTPETDAAGDKKPLSEQAVADWLLAHPEFFQEHRKLLTRLSIPH
                     PAGGAISLVERQVEILRQETRQLEQRLVDWMEIARDNDRLLARLHALGVALLAADERS
                     QRIAILDRRLREDFGSAAVALLLHGDAQAGDAAPLRRLAAKDPALEGLRESLAGGRPF
                     CRALSDHWRSALFGGDADLASAAFVPLGGNGELGLLALASREATHFHPGLDTTYLARL
                     GELATAALGGGFA"
     gene            complement(5412..6236)
                     /gene="dapF"
                     /locus_tag="VFX38_05565"
     CDS             complement(5412..6236)
                     /gene="dapF"
                     /locus_tag="VFX38_05565"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011627814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX5314357.1"
                     /translation="MRFRKMHGLGNDFVVVDATRAPVEIPLSRLAELADRHRGIGFDQ
                     WLVLEPGASRGEFGYRIFNADASPAEQCGNGLRCLARYAFERGYASGDAVRFGNGETT
                     QEARLAPGDLISVDMGRPVLEPSRIPFAAAAQAASYPLEVNGLRLTIGAVSMGNPHAV
                     LRVEDIAAAPVAELGPAIEHHPAFPQRANVGFLEVSAPSRVRLRVWERGTGETLACGT
                     GACAAMVWGRLMNWLEPLVEVELPGGVLELRWPGVESSIWATGPAVTVFEGELRDA"
BASE COUNT          997 a         2130 c         2262 g         1026 t
ORIGIN      
        1 gatattcggc gacgttctcg ttcaggcgat cgcgggttcc ttcgagggac tttttgagcg
       61 cacccgcgac tcgttcgagc gtccgcgacg ggcccgcacc gaggaccgtc gcgacgcgtt
      121 cccaggcggc gcgggggaga cgcgccatga cccgcgcgag actctggacg acttcggcgt
      181 cgccggcgac cgtgagacct cgcggattgc cgctgcgccc ggcggcgatg agtgcgctca
      241 tgcgcccgct cacggtcgcg tccgcgccct cgggcgtgcc gccgagttcg atgcgctcgc
      301 ccgcgatcgc gagcggcacg acgagcgaga gatcgctgat ttccacggcg acgcgacgcc
      361 cggaaagcgt gcggagagtg cgcagcgcct cgggatcggc ggcgagggcg gcgttgagcg
      421 cccgcgtcgc cgggtcgaga acaagcgcgg gaagcgtgcg tgtttcgctc acagtttcca
      481 tccgacgtga agggcgacga tgccgcccgc ctgattgtaa tagcgcacgc gctcgaaacc
      541 ggtcgcttcc atcatcttgc gaagttcctc ctgcgccgga tgacggcgga tggattcggc
      601 gagatagcga tagctcgcct cgtccttcgc gatccagcgc ccgagccgtg gcagcaccgt
      661 gaaggagtaa agatcgtagg cgcggcgaag cggctcgtac gggcgggaga actcgagcac
      721 cagcgcccgg ccgccgggag cgagcacgcg ccgcatttcc gcgagcgcaa gctccttgtg
      781 cgtcacgttg cgcaggccga agccgatagt gatgcagccg aaatgatcgg aagggaaggg
      841 cagggcctcg gcgttcgcga ccgtgcagcg cacgccgccg acgccggcgt cgagcaggcg
      901 ctcgcgtccg cggcggatca tggcggcgtt gatgtcggcg agtaccacct cccccgcggg
      961 gccgacctgg cgggcgagtc cgcgggcgag atcgccggtg ccgccggcca gatcgagcgc
     1021 ccgatcaccc ggtcgcagcc ccgtctgcag cagcgcgcaa cgcttccaca ggcgatgcag
     1081 gccgccggac atgaggtcgt tcatgagatc gtagcggggc gcgacggatt cgaacacggc
     1141 gcgcacgcgc cgcgtctttt ccgtcggcga cactctgcgg tagccgaagt cggtctggtt
     1201 ttcctgcatc gccgggtgct tccgggcctg ttctcattct acggtagcct gtgcccgcac
     1261 cggaggatgc ggtgagtcat cgaagcgcat cggcaaggcg tcctggtttc gatacggttc
     1321 gcaagctcac cgcacccggc gtcttcagcc tttcctgccg atgcccgcgc gttcgagccg
     1381 ggcgcggtaa tcctgccagt tgtgctcgaa gttctcgccg agcgtgcgca gaatccgcca
     1441 ggaataaagc cccgagtcgt ggccgtcgtc gaagacgatg cggaccgcat actggccgac
     1501 gggctcgacc gcgctcacgc cgacgttttc cttgccggcg acgagaagcc gcggcgctcc
     1561 gccgtggccc gtgacctcgg ccgagggcga gaagacgcgc agatattcga agggaagcgt
     1621 gaactcggcg ccgtcgtcga agcgcagata gagatcgcgg ctcgcgcgcc gcccgcggat
     1681 ttcgatcggg cgcggggctt cgccctcgct cacaggatgt agcgcgagag atcctcgtcg
     1741 tcggcgaggt ccccgaggtg cgcatcgacg tacgccgcgt ccacctcgac gctgctgccg
     1801 gcgcgatcgg gtgcctcgta ggagacggtt tcgagaaggc gctgcatcac cgtctgcagg
     1861 cgccgcgcgc cgatgttttc ggtgtgctcg ttcacgcggt gcgcgatctc cgcgatgcgg
     1921 cgcacgccgt cctcgcggaa ggcgagcttc acgttttcgg tcgcgagcat cgcgacatac
     1981 tgcaccgtga gcgcggctcg cggttcggtg agaatgcgaa tgaagtcggc gacgccgagc
     2041 gcactcagtt ccacgcggat cggaagccgc ccctgaagtt cgggaatgag atcggacggc
     2101 ttggcgacgt gaaaggcccc cgaggcgatg aagagaatgt gatcggtgcg caccatgccg
     2161 tagcgggtgt tgacggtcga gccctcgacg agcggcagaa gatcgcgttg cacgccctcg
     2221 cgcgaaacat cggcgcccga cacgccgccg tcgcgggcgc tgcgtgcgac cttgtcgatt
     2281 tcgtcgatga agacgatgcc gttttcctcg acctcgcgga tcgcgtcgcg acggatttcc
     2341 tcttcgttga cgagcttcgc cgcctcgtcg tcggcgagca gacgcctcgc ctcggcgacc
     2401 cgcacgcgcc ggttttcacg ccgctgtccg gtcatgccgc gcagcatgtt ttcgagctga
     2461 ccggtcattt cttccatgcc gggaggcgcg agaatttcca tgctcggctg cgccgcgctc
     2521 acttcgattt cgatttcgcg ctcgtcgagc gctccggcgc gaagctggcc gcgaatcttc
     2581 tggcgggtct cgtcgtgttc cgtcggcggg cgtggttcgt tggcgaagcc gaccgcatgt
     2641 gagcgtggca gcagcgcatc gaggagacgc tcttcggccg cctgctcggc gcgcacgcgc
     2701 accgcctcct tcgcctcgcc tcgcgccatc ttgatcgcga tttccgccag atcgcggatg
     2761 atcgaatcca cctcgcggcc gacgtagccg acctcggtga acttggtcgc ctcgaccttg
     2821 aggaaaggtg catgcgcgag acgcgcgagc cgccgcgcga tttcggtctt gccgacgccg
     2881 gtcggcccga tcatgagaat gttcttcggc atcacctcgg cacgcagttc cgccgggagc
     2941 tgcatccgcc gccagcgatc gcgaagcgcc acggccacgg cgcgtttcgc cgccgtctgc
     3001 ccgacgatgt gacgatccag ttcggcctcg atttcccgcg gagtcatcgt gtccgtcatg
     3061 cgcttgcggc ctcctccggc agggtctcga tcgtgatctg cgggttggta tagacgcaga
     3121 tttcggccgc gattccgagc gctgcgcgtg cgatcgcggg ggcggcgagc gtcgaattgc
     3181 gcagaagagc gcgggcggcg gcgagggcgt aaggaccgcc cgagccgatc gcaagcacgc
     3241 cttcctccgg ttcgatcacg tcgccgttgc cggagatcag cagcgaagtt tcggcgtcgg
     3301 cgacgaggag caaggcttcc aggcggcgca gcacgcggtc gctgcgccac tcgcgggcga
     3361 gcgcgaccgc cgcgcgggcg aggttgccgc tggagcgttc gagctgcgat tcgaacttct
     3421 cggcgagggt gaaggcatcg gcggtgccgc cggcgaagcc cgcgagcacc cggccttcgg
     3481 cgaggcgccg gaccttgcgg gcattggcct tgagcaccgt ctcgcccatc gagacctggc
     3541 cgtcgcccgc catcgcgacg acgccgtcgc gacgcgcgca gagaatggtg gtggcgtgaa
     3601 actgctgcat tcaggctcct ttcgcggccg atttcacgga cattcggcgc acagccgcaa
     3661 taaacgcaag tccaggattc gagcggtcat tcactcactt cgttcgctgt cagtcggtcg
     3721 cttcgcgacc agcaagcaag ttggctggag cgcggctatc cacacttcct cacttgctca
     3781 cagcgccgga ggcgcgactc cccggcggcg aagccgcgga ctcacggttt tttccgatgg
     3841 gcccgcgggt gtgcctgatc ataggtgcgg gcgaggtgct ggaaatcaag gtgggtgtag
     3901 acctgggtcg tcgcgagact cgcgtggccg aggagttcct gcacggcgcg cagatcgccg
     3961 ctcgattcca gcacgtgggt cgcaaaggcg tggcgcaaca gatgcggata aacgcggcga
     4021 tcgagcccct ggcgccgacc ccaatagctg atgcgggcct cgatcgaacg gcgggcgagg
     4081 cgccgtccgc gacggctgag aaagagcgcg gcctcgccgg gggcggcgaa ggcgagccgg
     4141 gccttgagcc agtcgccgag tgcctcgcgg gccttgctcc cgacgggaac gacgcgggtc
     4201 ttctcgccct tgccggtaac gcgcacgagt ccttcgcgaa gatcgagatc gttgagatcg
     4261 agcccggcga gttcggcgag acgcaggccc gaggaataaa agagttcgag tatcgcgcgg
     4321 tcgcgcccgg cctcgaagct cgcgcccgca tccacctcga cgagccgcgc cgcctcgtcc
     4381 acgtcgagcg ttgcgggaag cgtgcggcgc agtttcggcg tgcgcacgcc gatggtgaag
     4441 tcgcgttcga ggccgccgcg cttgagcccg tggcgcgcga agctgcgggt cgcggcgagc
     4501 gcgcgcgaaa ggctgcgggc gctgagtccg tcgcgatggc gctcggcaag atagcggctg
     4561 agacccgcgg gttcgagcgc gctccaatcg ccgattccgt tcgcgtcgca ccaggccgcg
     4621 agggctgcga gatcgcggcc gtaaccgccg atcgtgtgcg gggaaagtcg tcgctcgaag
     4681 ccgaggtgtt cgaggaatcg gcggcggaac ccctcggcgt cggggctcaa gcgaagcctc
     4741 cgccgagcgc cgccgttgcg agttcgccga gacgtgcgag ataggtggtg tcgagccccg
     4801 gatgaaaatg cgtggcctcg cggctcgcga gtgcgagaag acccagctcg ccgttgccgc
     4861 cgagcggcac gaaggcggct gaggcgaggt cggcatcacc gccgaagagc gcgctgcgcc
     4921 aatgatcgga aagggcgcgg caaaagggcc gtccgcccgc gaggctttcc cggagtcctt
     4981 cgagcgcggg atccttcgcc gcgagccggc gaagcggagc cgcgtcgccc gcctgcgcgt
     5041 caccgtggag aaggagcgcg accgcggccg agccgaagtc ctcgcgcaga cgccggtcga
     5101 gaatcgcgat gcgttgcgaa cgctcgtcgg cggcgagaag cgcgacgccg agcgcgtgca
     5161 ggcgggcgag caggcgatcg ttgtcgcggg cgatttccat ccagtccacg aggcgttgtt
     5221 cgagctggcg ggtctcctgg cggagaatct cgacctggcg ctcgacgagc gagattgcgc
     5281 cgcccgccgg atgcgggatg ctgagacgcg tgagaagttt tcggtgctcc tgaaaaaatt
     5341 cgggatgcgc gagcagccag tcggcgaccg cctgctccga cagcggcttc ttgtcgccgg
     5401 cagcatcggt ctcaggcgtc acggagttct ccctcgaaca cggtgaccgc cgggccggtc
     5461 gcccagatcg aactctcgac gccgggccag cgcagttcga gcacgccgcc cggaagctcg
     5521 acctcgacca acggttccag ccagttcatg agccgtcccc agaccatcgc ggcgcaggcg
     5581 ccagtgccgc aggcaagggt ttcgccggtg ccgcgctccc acacgcgcag gcgtacccgc
     5641 gacggcgccg acacttcgag aaagccgacg ttggcgcgtt gcggaaaggc gggatggtgt
     5701 tcgatcgcgg gtccgagttc ggcaacggga gccgcggcga tgtcttccac tcgcaggacc
     5761 gcgtgcggat tgcccatcga aaccgcgccg atcgtgagcc tgagcccgtt cacctcgagc
     5821 ggataacttg cggcctgcgc ggcggcggcg aagggaatgc gcgaaggttc gagcacgggc
     5881 cggcccatgt ccacgctgat gagatcgccg ggcgcgagcc gggcctcctg cgtcgtttcg
     5941 ccgttaccga agcgcaccgc atcaccactt gcgtagcccc gctcgaaggc atagcgggca
     6001 aggcagcgca ggccgttgcc gcattgctcc gcggggctcg cgtcggcgtt gaagatgcga
     6061 tagccgaatt cgccgcggct cgcgcccggc tccagcacca gccactggtc gaagccgatg
     6121 ccgcgatggc ggtcggcgag ttcggcgagg cggctcagag gaatctcgac cggcgcccgg
     6181 gtggcatcca ccaccacgaa gtcgttgccg agcccgtgca tcttgcgaaa ccgcatcgcc
     6241 gcccctgcgc cgtttgccgc attataggga tcggcgccgg cggccgacag gcttcccggg
     6301 cggggcggcc ttcgtggcgg gcgtggtccg ccgccgttat actgcccttc gggtccgcaa
     6361 gaaggaaaca acgatgcgta ccatcctgat cgccaatgcc aaagggggct gcggc
//
LOCUS       DASUJG010000259         4874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_810062, whole genome shotgun sequence.
ACCESSION   DASUJG010000259 DASUJG010000000
VERSION     DASUJG010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810062"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1386
                     /gene="guaA"
                     /locus_tag="VFX38_05570"
     CDS             <1..1386
                     /gene="guaA"
                     /locus_tag="VFX38_05570"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019591194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX5314358.1"
                     /translation="PASAAGRRPPAAPAVIFELGVPILGICYGMQTLAAQLGGRVEAS
                     GEREFGYARLGLEDAGRLLGGIEDRPGELDVWMSHGDRVLAPPPGFSVLAATPAAPVA
                     AMADESRRYYGLQFHPEVTHTPQGRRILERFLREICGASGAWTTGNIIASHVAAIRER
                     VGDDEVIMALSGGVDSAVAAALIHRAIGNRLHPVFVDHGLLRSGEADQVERAFAQHLG
                     VTLTRVDAAGRFFAALVGIADPEEKRRRIGHAFIEVFEEQAARLPEVGWLGQGTIYPD
                     VIESAAAGKGAAEIIKSHHNVGGLPERMRLKLLEPLRDLFKDEVRRIGLELGLPAELL
                     GRHPFPGPGLAVRVLGEVSRERVELLRRADAIFLEALSGAGLYDQVAQAFAVFLPVRA
                     VGVMGDQRRYEYVIALRAVETSDFMTAHWARLPYEFLDEVARHIINRVPGIARVVYDI
                     SGKPPATIEWE"
     gene            1601..2035
                     /gene="tadA"
                     /locus_tag="VFX38_05575"
     CDS             1601..2035
                     /gene="tadA"
                     /locus_tag="VFX38_05575"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007152456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEX5314359.1"
                     /translation="MRRALRLARRAGEAGEVPIGALVIRDGRLLGAAANAPIGTCDPT
                     AHAEILALRRAARRAGNYRLPGATLYVTLEPCAMCTGAIVHARIARVVYGADDPRAGA
                     VRSVFRLLDDARLNHRAECTGGIEAAASAALLQAFFRRRRGE"
     gene            complement(2175..3587)
                     /gene="mltF"
                     /locus_tag="VFX38_05580"
     CDS             complement(2175..3587)
                     /gene="mltF"
                     /locus_tag="VFX38_05580"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008112.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HEX5314360.1"
                     /translation="MALRVSQAVLVAAAFCLLATFAHSPSLLSRIQNAGVLVLATPNS
                     PTTYYLAAYGPSGPDYDLGRRLAKELGVRLEVLQVANGDQALRAVAAGRADIAAPGVA
                     VRPADFPYLSFAPPYQMVSQVLVYRTGETVPRSLATLGSSGLRITVAPGYQPLLRQLA
                     RTHPGLEWQVKPRSGADELLVDVAEGRARYTVVSENEFRLNRQFYPQLQAAFPLTDEQ
                     PLAWAVGGGADTSLYQATVAFFARAQADQQVTSILGRYYGNRDTYDPLRAEGFLNDLG
                     SRFADYASSFERAASAADMPWQLLAAVGYQESHWNPRARSPTGVRGMMMLTEDTAQKL
                     GVSDRSDAHLSIRGGARYIGMLRARLPASIPEPDRTWMALAAYNIGYAHILDALALTK
                     KMGWDPDRWADVKRALPLLAERRYYRDIHYGYANGEQAVRYVTNIRSYYEILAWRSAQ
                     NSLPRQVSDSLAAAVTRPAL"
     gene            3756..3845
                     /locus_tag="VFX38_05585"
     tRNA            3756..3845
                     /locus_tag="VFX38_05585"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3790..3792,aa:Ser,seq:cga)
     gene            complement(4004..4795)
                     /locus_tag="VFX38_05590"
     CDS             complement(4004..4795)
                     /locus_tag="VFX38_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014403864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HEX5314361.1"
                     /translation="MGFLAGKRALIVGLASQRSIAWGIAGSMHREGARLAFGYQGEKL
                     AERAREMACEVESSAVFECDVSSDEDIERLVAGAGQALGGLDILVHSVGFAPREALAG
                     GFTESTTREAWRIAQDVSAYSLVALARAARPMLADAHGSILTLSYLGAVRALPNYNVM
                     GPAKASLEAAVRFLAQDLGPEGIRVNAISAGPIKTLAAAGIAGFRGMLSHVAQTAPLG
                     RSVSAEEVGDAAAFLCSDLARGITAETLYVDAGYHAVAMTLPNER"
BASE COUNT          755 a         1590 c         1708 g          821 t
ORIGIN      
        1 cccgcctcgg ccgccggccg gcgtccaccc gcggctcccg ccgtgatctt cgaactcggc
       61 gtgccgattc tcggaatctg ttacggcatg cagaccttgg cggcgcaact tggcggccgc
      121 gtcgaggcga gcggcgagcg cgagttcggc tacgcgcgcc tcgggctcga ggacgccggc
      181 cggttgctcg gcggcatcga agatcgtccc ggcgaactcg acgtgtggat gtcgcacggc
      241 gaccgcgtgc tcgcgccgcc gccgggcttt tccgtgcttg cggcgacgcc cgccgcgccg
      301 gtcgcggcga tggcggacga atcgcgacgc tattacggtt tgcagtttca ccccgaggtc
      361 acgcacactc cgcagggccg gcggattctc gagcgcttcc tgcgcgagat ctgcggcgcc
      421 tccggcgcct ggaccaccgg caacatcatc gcctcgcatg tggcggcgat tcgcgagcgc
      481 gtcggcgacg acgaagtgat catggcgctt tcgggcggtg tcgattccgc cgtcgcggcg
      541 gccctcattc atcgcgcgat cgggaatcgc ctgcatccgg tgttcgtgga tcacggcctt
      601 cttcgctccg gagaagccga tcaggtcgag cgcgccttcg cgcaacatct gggcgtgacc
      661 ctgacccgcg tggacgccgc cgggcgattc ttcgctgcac tcgtcggcat cgccgatccc
      721 gaggaaaagc gccggcgcat cgggcatgcc ttcatcgagg tcttcgaaga gcaggcggcg
      781 cggctgccgg aggtcggttg gctgggccag ggaacgattt atccggacgt gatcgaatcg
      841 gcggccgcgg gcaagggcgc ggccgagatc atcaaatcgc atcacaacgt cggagggctt
      901 cccgaacgca tgcgcctgaa gctgctcgaa ccgctgcggg atctgttcaa ggacgaggtg
      961 cggcgaatcg gcctcgaact cggactgccc gccgaactcc ttggccgtca tcccttcccc
     1021 ggccccgggc ttgcggtacg ggtgctgggc gaggtcagcc gcgagcgggt ggagttgctg
     1081 cggcgcgcgg atgcgatttt cctggaggcg ctttccgggg cgggactcta cgaccaggtc
     1141 gcgcaagcct ttgccgtgtt tttaccggta cgcgccgtag gtgtcatggg cgatcagcgc
     1201 cggtacgagt acgtgatcgc gctgcgcgcc gtggagacga gcgatttcat gaccgcgcac
     1261 tgggcgcgcc ttccgtatga atttctcgac gaagtggcgc ggcacatcat caaccgcgtg
     1321 ccgggaatcg cccgcgtcgt ctacgacatc tccggcaagc cgccagccac gatcgagtgg
     1381 gaatgaccgg ggaagtgcgt cggcggctgc gccgcctggt agtcgcgcct tcggcgctgc
     1441 cagcaggcaa gaacatcggt gtctttccga cttgcttgca ggccgcgaag cggccgactg
     1501 acaccgagcg ccttgccttt cgtgcttgag tacagaattg gcggtggaag aagaaatctc
     1561 gattgccgcc ttccatcccg gtaacgccga tcggcgctgg atgcggcgcg cgctcaggct
     1621 cgcgcgccgc gcgggcgagg ccggcgaagt gccgatcggc gccctcgtca tccgggacgg
     1681 acggctgctc ggcgcggcgg ccaacgctcc gatcggcacc tgcgatccga cggcccatgc
     1741 cgaaattctc gcgctgcgcc gcgccgcccg acgcgcaggc aattatcgcc tgccgggagc
     1801 gacgctctat gtcaccctcg aaccctgtgc gatgtgtacc ggcgccatcg tgcacgcgcg
     1861 catcgcccgc gtggtttacg gcgccgacga tccgcgtgcc ggcgccgtgc ggagcgtctt
     1921 tcgcctgctc gacgatgccc gtctcaacca ccgcgccgaa tgtaccgggg gcatcgaggc
     1981 cgccgcgagc gccgcattgc tgcaagcctt ctttcgccgc cgaagaggtg agtgagtgag
     2041 gaagtgagaa agcaagtcag caagtcgggg aagaggggcg aacgtgtact gctgacgtac
     2101 ttgcagccgc gtagccgcga cttaccgcga gcaccgcgag tggcttgcca gcgccgaaga
     2161 cgcgacttta cggctcagag agcggggcgg gtcaccgcgg cggcgaggct gtcggagacc
     2221 tgcctcggca gactgttctg tgcgctgcgc caggcgagga tttcgtaata gctgcggatg
     2281 ttcgtgacgt agcgaacggc ttgttcgccg ttggcgtagc cgtagtggat gtcgcggtag
     2341 tagcggcgtt cggcgagcag cgggagtgcg cgcttgacgt cggcccagcg atcgggatcc
     2401 cagcccattt ttttggtgag cgcgagtgcg tcgaggatgt gggcatagcc gatgttgtag
     2461 gcggcgagtg ccatccaggt gcggtccggc tcggggatgc tcgcgggaag ccgcgcgcgc
     2521 agcatgccga tgtaacgcgc gcccccgcga atgctcagat gcgcatccga gcgatcgctc
     2581 acgccgagct tttgggccgt gtcctccgtg agcatcatca tgccgcgcac gccggtcggt
     2641 gatctcgcgc ggggattcca gtgtgattcc tgatatccga ccgccgcgag caactgccag
     2701 ggcatgtccg ccgccgacgc ggcgcgctcg aaactcgagg catagtcggc gaagcggctg
     2761 ccgagatcgt tgagaaagcc ttcggcccgc aggggatcgt aggtgtcgcg gttgccgtaa
     2821 tagcgcccga ggatgctcgt cacctgctgg tccgcctggg cgcgggcgaa gaaggcgacc
     2881 gtcgcctgat aaaggctggt gtccgcgcct ccgccgacgg cccaggcgag cggctgttcg
     2941 tcggtgagcg ggaaggcggc ctgcaactgc gggtaaaact ggcggttgag acgaaactcg
     3001 ttttcgctca cgaccgtata ccgggcccgg ccctcggcca cgtccacgag cagttcgtcg
     3061 gccccggatc ggggcttgac ctgccactcg agtccggggt gcgtcctcgc gagctggcgc
     3121 agaagtggct ggtacccggg cgccacggtg atccgcagtc ccgaagaccc gagagtcgcg
     3181 aggctgcggg gcacggtctc tcccgtccgg tacacgagca cctgcgagac catctgatag
     3241 gggggggcga agctcaagta cgggaaatcg gccggtcgca ccgctacgcc cggagccgcg
     3301 atgtcggccc ggcccgccgc caccgcccgc agtgcctgat cgccattcgc gacctgcaac
     3361 acctcgaggc gcacgcccag ttccttcgcc aggcggcggc cgagatcgta gtccggcccg
     3421 ctcggcccat aggcggccag atagtaggtg gtcggactgt tcggggtcgc gagcaccagc
     3481 acgccggcgt tctgaatgcg actgaggagg ctcggagaat gggcaaaggt cgcgagcagg
     3541 cagaaggccg ccgcgaccag aaccgcctgg gagacgcgca gcgccaagct tcccgctctc
     3601 gcgcgagcct gccaccgatt gagcttcctg aagcgtttca agggcttggc cgaattccgc
     3661 aagcgcctag tctacccggt ttcaaggcag ccttgcttcc gccgtctggt atgcttatac
     3721 gttcgccgcc cggattcccg gcgcggacgg gccgcggaga ggtgccagag cggccgaacg
     3781 gggcggtctc gaaaaccgtt gtgcccttcg gggcaccgtg ggttcgaatc ccaccctctc
     3841 cgccaactgc ctactgaaag ctactgcgct cgacggattg ggcgcgggcg gtgctcgaca
     3901 tcctcatgta cgggtctgta cactgcggtg tctgcgctcc ggccgcgccc aatccgcctt
     3961 cgctcgctac gctttcagcg ggcagttctc tgggtcttgc cccttacctt tcgttcggca
     4021 gggtcatggc gacggcgtga tagccggcat ccacatagag cgtctcggcg gtgatgccgc
     4081 gggcgagatc ggagcacagg aaggccgcgg cgtcgccgac ttcttccgcg ctcacgctgc
     4141 gtccgagcgg cgcggtttgg gcaacgtgag agagcatgcc gcgaaagccg gcgatgccgg
     4201 cggcggcgag cgtcttgatc gggcccgccg agatggcatt gacgcgaatg ccttcggggc
     4261 cgagatcctg ggcgagaaag cgtacggcgg cctcgaggct cgccttcgcg gggcccatga
     4321 cgttgtaatt cggaagcgcg cgaacggcgc cgagatagct cagcgtgaga atgcttccat
     4381 gcgcgtcggc gagcatcggg cgggcggcgc gggccagggc aacgaggctg taggcactca
     4441 cgtcctgggc gatgcgccag gcctcgcggg tcgtggattc ggtgaagccg ccggccagcg
     4501 cctcgcgcgg ggcgaagccg acggagtgca ccaggatgtc gaggccgcca agagcctgtc
     4561 cggcgccggc aacaagccgc tcgatgtctt cgtcactcga gacgtcgcat tcgaaaactg
     4621 cgctggattc gacctcgcag gccatttccc gcgcccgctc ggcgagtttc tcaccctggt
     4681 agccgaaggc gaggcgggcg ccctcgcggt gcatggagcc ggcgatgccc caggcgatgg
     4741 aacgctggct cgcaagtccg acgatcagcg cccgtttgcc ggcaagaaat cccactcgca
     4801 gggctctttc ggctagttgc tggcgctgac gaacagggtc aacaactggc cgggatggag
     4861 gatgctcgcg gccg
//
LOCUS       DASUJG010000260         4705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_810136, whole genome shotgun sequence.
ACCESSION   DASUJG010000260 DASUJG010000000
VERSION     DASUJG010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4705
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810136"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..2489
                     /locus_tag="VFX38_05595"
     CDS             27..2489
                     /locus_tag="VFX38_05595"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HEX5314362.1"
                     /translation="MRKHTWMIAVVALAVLLPGLALPLGLGPIHSTTRIGQPLEARIA
                     IIGADSATPQGLVVTLADASAHRQAGLSEPDFLFHLKFAVKQDASGTYVLVSSQQPVT
                     LPFLNLLIHASWGNGELTRQYTLLLNPPAFASGGQGAAAGTPAAPPAAAAASPSPPVP
                     AARTVAVQSSATAEPVRLAPPKVEASPTIAAASVPPVADSSDPESAASASLASASPLP
                     PAAIARSSARSTFVRSGSRSSADAPAAKPAPAEPAIVTAAVPVSSGTAEASPSSPGTF
                     GPVTYGSTLWSIASQLRPAGVSVKQMMMALYHANPQAFAGNIYRLYAGVKLRIPAADD
                     IRLVSSSEAADEVAAQEHARRSAAPAPLTTGIAAVAAVANASAQPAASAALAATEAAN
                     SATAKTTAGVAVAAAVGATAAATARSVAASAVAASAAGSAAAIAGAMLARGGASNAAS
                     AAAAGSLAIAAVQGNPPGKPAGATHPQAGGAAGLAGSAAPQSVPAGARAQGTIQNWLN
                     SPKGWIVIALIVLLLIILAVYFLHRRSAARDLEFAREPAAPASVATMEDAADQPVAAV
                     AKSEEPAGESLPIEVAAPIVAPSPQPIAASWADDSAARLREALAVEPGRQDLRRKLLE
                     LLFAAEDREGFRVQAQRLHDESAGEGSDWDAVAAMGRQLLPGDGLFAQDAPSHWNSVA
                     AEGAEAMPTGFAESAAMTVPGDGSENVRDEFSSFVENYVPGARTPANPPEGEAGGEDD
                     DGAFEREGYGEIPESSRAAAAAALAALLASAPAEPSHPDGEGRENAKLRLDLARAYLK
                     LGYPNAAREVLEEVLGEVATPA"
     gene            2512..3390
                     /gene="truA"
                     /locus_tag="VFX38_05600"
     CDS             2512..3390
                     /gene="truA"
                     /locus_tag="VFX38_05600"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019583868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX5314363.1"
                     /translation="MSVRRLAAILEYDGGDFIGWQRQTTGRSVQAEVESALGKVADEG
                     VTVVCAGRTDRGVHAAAQVLHFETSRERSPRSWLLGTNASLPPDVSLIAVREMPAEFH
                     ARFSAIARVYRYIVCERPTRPALLRRRVAWTHRRLDVPRMQEAAAALIGEHDFSAFRG
                     ADCQARSPLRRLERIAVTRHESLVAIEVKANAFLLHMVRNIAGSLLLVGSGRAEPRWL
                     EEVLRKRDRRLAGPTAPARGLTLVGVEYPAACGIRVAAGNAGASIEALEMLGYHGRDA
                     GGAPAPRPGTEGNEAR"
     gene            3387..4250
                     /gene="accD"
                     /locus_tag="VFX38_05605"
     CDS             3387..4250
                     /gene="accD"
                     /locus_tag="VFX38_05605"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEX5314364.1"
                     /translation="MSWLRKLVPSRIESDGRKTRRSVPEGVWVRCPACGAMLYRAELE
                     RNLKVCPKCDHHLRMGSRERIASFLDAEPRTEIGADVAPVDFLHFRDSKRYRDRLVQA
                     QKATGERDALVVVQGKLKGIPVVAAAFEFGFMGGSMGSVVGERFVLAVETAIRSGSAF
                     VCFSASGGARMQEALTSLLQMAKTSAALAALREKHLPFVSVLTDPTTGGVSASLAMLG
                     DLNIAEPKALIGFAGPRVIEQTVRETLPEGFQRSEFLLEHGALDQVVDRRELRERIAG
                     LLGMLLKKNPG"
     gene            4247..>4705
                     /locus_tag="VFX38_05610"
     CDS             4247..>4705
                     /locus_tag="VFX38_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEX5314365.1"
                     /translation="MSDAEAALATWLARLELRDPVRIEPGLERVATALTRMGLKPPPF
                     PLLTIGGTNGKGSVATYLTAFLRAEGEDVVGTYTSPHLGEYRERIAVDGRPIEAAPLV
                     RAFEAAQAGAEGLSLTYFEFGTAAALAAFRDAGVRVAVLEVGLGGRLDAVN"
BASE COUNT          694 a         1591 c         1667 g          753 t
ORIGIN      
        1 tggcaggggg agcgatgaca aagggaatgc ggaaacacac gtggatgatc gcggtggtgg
       61 cgcttgccgt gctgctgccg ggcctcgcgc ttcctctggg actcggcccg attcattcga
      121 cgacgaggat cggccagccg ctcgaggcgc gaatcgcgat catcggcgcc gacagcgcaa
      181 ccccgcaggg cctcgtcgtg acgctcgccg acgcctccgc ccaccggcag gcaggcctga
      241 gcgagccgga ttttctcttc cacctcaagt ttgcggtgaa gcaggacgca agcggtacct
      301 acgtactggt cagttcgcag cagccggtca cgctgccgtt tctgaacctt ctcatccatg
      361 cgagctgggg caacggggaa ctgacgcgcc agtacacgct gctgctcaat cccccggcgt
      421 ttgcctcggg cgggcaggga gccgctgccg gcacgcccgc ggcaccgccg gcggcggccg
      481 ccgcttcccc gtcgccgcct gtgccggccg cacggaccgt tgcggtgcag tcttcggcaa
      541 cggcggaacc ggtgcgtctt gcgccgccga aggtcgaggc aagcccgacc atcgcggcgg
      601 cctcggtgcc gcccgttgcg gattcgtcgg acccggaatc ggcagcgtct gcgtccctcg
      661 cttcggcgtc gccgcttccg ccagcggcca tcgcccgaag ttccgcgcga agcacctttg
      721 tgcggagcgg ctcgcgctcc agcgccgatg cgcccgcagc gaaacccgcc ccggcggaac
      781 ccgccatcgt gaccgccgcg gtgccagtgt cttccggtac cgcggaggca tcgccgtctt
      841 cgccggggac tttcgggccg gtgacctacg gctcgacgct atggagcatc gcctcgcaat
      901 tgcgtcccgc cggggtcagc gtcaagcaga tgatgatggc gctgtatcac gcgaatcctc
      961 aagcctttgc gggcaacatc tatcgactct atgccggagt gaagctgagg attccggcgg
     1021 cggacgacat ccgcctggtt tcttcttccg aggcagccga tgaagtcgcc gcccaggagc
     1081 acgcccggcg ttccgccgcg ccggctccac tgaccaccgg gattgcggcc gtcgcggcgg
     1141 tggcgaacgc ttccgcgcag ccggcggcga gcgccgcgct tgccgcaacg gaggctgcga
     1201 actccgccac cgccaagacc acagccggcg tcgccgtggc ggccgctgtg ggcgcaaccg
     1261 ctgccgcgac tgcccgctcg gtggcggcgt ctgccgttgc agcaagcgca gcgggatccg
     1321 ccgcagcgat tgccggcgcg atgcttgcgc gcggtggcgc cagcaatgcc gcgtcggcgg
     1381 cggccgcggg cagccttgcc attgccgcag tccaggggaa tcccccgggc aagccggcgg
     1441 gagcgactca tccgcaggcg ggcggagccg ccggtcttgc gggatcggca gcgccgcagt
     1501 ccgttccggc gggcgcgaga gcgcaaggca cgatccagaa ctggctcaac agtccgaaag
     1561 gctggatcgt gattgccctt atcgttctgc tgctcatcat tctcgccgtg tactttctgc
     1621 accggcgcag cgccgctcgc gatctcgagt ttgctcgcga acccgccgct cccgcgtcgg
     1681 tcgccacgat ggaggacgcg gcggaccagc cggtggccgc ggttgcaaaa tcggaggagc
     1741 cggcgggcga gtcgcttccc atcgaggtcg cagcgccgat cgtggctccg tcgccgcagc
     1801 cgattgccgc gagttgggcc gacgattcgg cggcgcgatt gcgcgaagcg ctggcagtcg
     1861 aacccgggcg ccaggatttg cgccgcaagc tgctcgagct gctgttcgcg gccgaggatc
     1921 gcgagggatt ccgcgtgcag gcgcaacgcc ttcatgacga gtcggcggga gagggatccg
     1981 actgggacgc cgtggcggcg atgggccggc aactgctgcc cggggacggg ctgtttgcgc
     2041 aggatgcgcc ttcgcactgg aactccgtgg ccgccgaagg ggcggaggcg atgccgacgg
     2101 gcttcgccga gtctgcggcg atgacggttc ccggcgacgg ttccgagaac gtccgcgacg
     2161 agttttcgag cttcgtcgaa aattatgtgc cgggagcccg aacgccggcg aatccgcccg
     2221 agggcgaggc gggcggcgaa gatgacgacg gcgctttcga acgcgagggt tacggcgaga
     2281 ttccggagtc ttcccgggcc gccgccgcgg cggcacttgc cgcgcttctc gcatccgctc
     2341 ccgcggaacc gtctcatccc gatggagaag gtcgcgagaa tgccaagctg cggctggatc
     2401 tcgcgcgcgc ctatctcaag ctcggatatc ccaacgccgc gcgcgaggtg ctcgaggaag
     2461 ttctgggcga ggtggcaacg cccgcttgag cgtatcggac cggcggcttc catgagcgtg
     2521 cggcgacttg ccgcgatcct cgaatatgac ggcggcgact ttatcggctg gcaacgtcag
     2581 acgacgggcc gcagcgtgca ggcggaggtc gagtcggcgc tcggaaaagt cgccgacgaa
     2641 ggggtcaccg ttgtctgcgc cgggcgtacc gatcggggtg tgcacgcggc cgcccaggta
     2701 ctgcacttcg aaaccagccg cgagcgcagc ccgcgcagtt ggctgctcgg cacgaacgcc
     2761 agtcttccgc ccgacgtgtc cctgatcgcg gtgcgggaaa tgccggcgga atttcatgcg
     2821 cgcttcagcg ccattgcacg tgtctatcgc tatatcgtct gcgagcgtcc gacgcgcccg
     2881 gcgctgctgc gccggcgcgt ggcctggacg catcgtcggc tcgacgtccc gcgcatgcag
     2941 gaggcggccg cggccctgat cggcgagcat gacttcagcg cctttcgcgg ggcggattgc
     3001 caggcgcgat cgccgctgcg ccggctcgag cggatcgccg tcacgaggca tgaaagtctc
     3061 gtcgccatcg aagtcaaggc gaatgccttt cttctgcaca tggtgcgcaa tattgccggc
     3121 agcctgctcc tcgtcggctc cggccgcgcc gagccgcgat ggctggagga ggtgctgcga
     3181 aaaagggatc gcaggctcgc gggtccgacc gcacccgccc gcggtctgac tctcgtcggc
     3241 gtggagtatc ccgcggcctg cggcattcgc gtcgctgccg ggaacgccgg tgcatcgatc
     3301 gaagccctcg aaatgctggg ctatcatgga cgggatgccg gcggcgcgcc cgcgccgcgg
     3361 cccggaacgg aaggaaacga ggcgagatga gctggttgcg caaactcgtt ccgtcgcgaa
     3421 tcgaatccga cggacgcaag acgagacgca gcgtgcccga aggcgtgtgg gtgcgttgcc
     3481 cggcctgcgg cgcgatgctc tatcgcgccg agctcgagcg caatctcaag gtctgtccca
     3541 agtgcgacca tcacctgcgg atgggctcgc gcgagcggat cgcgagcttt ctcgatgccg
     3601 agccgcgcac ggagatcggc gccgatgtcg ctccggtgga cttcctgcat tttcgcgatt
     3661 ccaagcgcta ccgcgatcgc ctcgtgcagg cgcagaaggc gacgggcgag cgcgatgcgc
     3721 tggtcgtcgt gcaggggaag ctcaagggga ttccggtcgt cgctgctgcc ttcgagttcg
     3781 gcttcatggg cggttccatg ggttcggtcg tcggagagcg cttcgtgctc gccgtcgaga
     3841 cggcgatccg ttccggttcg gccttcgtct gcttcagcgc gagcggcggc gcccgcatgc
     3901 aggaggccct cacttccctg ctgcagatgg cgaagaccag cgccgcgctg gccgcattgc
     3961 gggagaagca tctgccgttc gtctccgtgc tcaccgatcc gacgaccggc ggcgtgtccg
     4021 cgagtctcgc gatgctcggc gacctcaaca tcgccgagcc caaggccctg atcggcttcg
     4081 ccggcccgcg cgtgatcgaa cagacggtgc gcgagactct gcccgaggga ttccagcgca
     4141 gcgagtttct gctcgagcac ggcgcgctcg atcaggtcgt ggaccggcgc gaactgcgcg
     4201 agcggatcgc gggcctgctc ggcatgctcc tgaaaaaaaa tccggggtga gcgacgccga
     4261 agcggcactg gccacatggc tggcgcggct ggaactgcga gatcccgtcc gcatcgaacc
     4321 cgggctcgag cgcgtcgcga cggcgctcac gcgcatgggc ctcaagccgc cgccgtttcc
     4381 gctgctcacg atcggcggga ccaacgggaa gggatcggtc gcgacctacc tcacggcctt
     4441 tctgcgcgcg gaaggcgagg acgtcgtcgg tacctataca tccccgcatc tgggcgagta
     4501 tcgcgagcgg atcgccgtgg acggccggcc catcgaagcg gcgccgctgg tgcgcgcctt
     4561 cgaggccgcg caggccggcg ccgaaggcct gtcgctgacc tatttcgagt tcggcacggc
     4621 ggcggcgctc gcggccttcc gcgatgcggg agtgcgcgtc gcggtgctcg aggtgggatt
     4681 gggcgggcgg ctcgatgccg tcaac
//
LOCUS       DASUJG010000261         2019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_688787, whole genome shotgun sequence.
ACCESSION   DASUJG010000261 DASUJG010000000
VERSION     DASUJG010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688787"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VFX38_05615"
     CDS             <1..269
                     /locus_tag="VFX38_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015828146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HEX5314366.1"
                     /translation="NIPFAEDLAAAYYCAADPATPTKVKGVLFAALAYFVMPFDAVPD
                     FIAGLGFTDDAAVLAMAIGLVSRHITNEHRKRARRTLGVAEPNA"
     gene            266..691
                     /locus_tag="VFX38_05620"
     CDS             266..691
                     /locus_tag="VFX38_05620"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX5314367.1"
                     /translation="MTYKEVEKFLKSLKHTTMVVQWGDHQVYKIGGKVFAVAGGSGDA
                     EPGLTFKAGETSFAILTKQKGIIQAPYMARNQWVKLERLSALKDSELKAYLERSYNMI
                     ASGLTKKMRVSLGFEQAPAKKAKAGKAKASAKTEAKKKA"
     gene            complement(688..1311)
                     /locus_tag="VFX38_05625"
     CDS             complement(688..1311)
                     /locus_tag="VFX38_05625"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX5314368.1"
                     /translation="MAADKEELVAKARAERRRYKRIPVSLSGRLFVPAESREAHCKIA
                     DMSPGGARIACEIVPPQGAQVVVYIDGFGRFEGTVARPDAGAFGIQFNCSQHKRERVA
                     EQLTLYLNGGDVNESVLRRHDRAPTKGLARFTRANGDVVNCEVLDLSLSGVSLSTDVR
                     PPVGEFVLIGQMAGRVARHHETGIGIEFVAQEKPVAEPAKPKLFVAR"
     gene            1536..>2019
                     /locus_tag="VFX38_05630"
     CDS             1536..>2019
                     /locus_tag="VFX38_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314369.1"
                     /translation="MTLAPSRTGLIRIAVVAAALALSACASNGLLDELDQTQPVGSDF
                     SKELFKDYAYLARSFGDAGNSASTAFDAGNSLSLSGDSSGEIADLATAYAQKALDAAQ
                     GQEVLPEAAPDGNADADQMRQRLLASLENGRSKFPADAARAQVQYDCWVMNARVESLR
                     S"
BASE COUNT          414 a          635 c          633 g          337 t
ORIGIN      
        1 gcaatattcc cttcgccgaa gacctcgccg cggcctatta ttgcgccgcc gatccggcca
       61 cgcccaccaa agtgaaaggc gtgctgttcg cagccctcgc ctatttcgtc atgccgttcg
      121 atgcggtgcc ggacttcatc gcgggactcg gtttcaccga cgacgcggcg gtgctggcga
      181 tggccatcgg cctggtgtcg cggcacatca ccaacgagca ccgcaagcgc gcgcgcagaa
      241 cgctcggtgt ggcggaaccg aacgcgtgac ctacaaggag gtcgagaaat tcctgaaatc
      301 gctcaagcac accacgatgg tcgtgcagtg gggcgatcat caggtttaca agatcggcgg
      361 aaaggttttc gcggtggccg gcggatcggg cgatgccgag cccggcctta cgttcaaggc
      421 cggcgaaacc agtttcgcca ttctcaccaa gcagaaggga atcattcagg cgccttacat
      481 ggcgcgcaat caatgggtga agctggagag attgtcggcg ctgaaagaca gcgagctcaa
      541 agcctatctg gaacgctcgt acaatatgat cgcatcgggc ctgacgaaga agatgcgcgt
      601 cagcctcggc ttcgagcaag cgcctgcgaa gaaagccaag gccggaaaag ccaaggcatc
      661 tgcaaaaacg gaagcgaaaa agaaagccta gcgggcgacg aagagcttgg gcttcgccgg
      721 ctcggcgacc ggcttttcct gcgcgacgaa ttcgataccg atgccggttt catgatgacg
      781 cgcgacgcgg cccgccatct ggccgatgag cacgaactcg ccgaccggag ggcgcacatc
      841 ggtcgatagc gaaacgccgg aaagcgaaag atcgagcact tcgcaattca caacatcgcc
      901 attggcgcgg gtaaaacggg caagcccctt ggtgggcgcg cggtcgtggc ggcgcagaac
      961 cgattcgttg acgtcaccgc cattgagata gagcgtgagc tgttcagcaa cgcgctcgcg
     1021 cttgtgctgc gagcagttga actggattcc gaacgccccg gcgtcggggc gcgcgacggt
     1081 gccttcgaag cgcccgaagc catcgatata gacgacaacc tgtgcgcctt gcggaggaac
     1141 gatctcacag gcaatgcgcg cgccgcccgg cgacatgtcc gcaatcttgc aatgggcttc
     1201 gcggctttcg gcgggcacga agaggcggcc cgaaagggag accggtatgc gcttgtaacg
     1261 ccggcgctcc gcgcgggctt tcgcgaccag ttcttccttg tctgctgcca tatgaacgcc
     1321 gcgcgaacag gcggctcccg gattcagcct cgaacggtgt ttgtagtcct ttaagggtca
     1381 atagaacgtt accgccgcac ccccgccatc agccttaagg aagggtaaac gccggggctt
     1441 aacctgcgga ccggcgcagg ctataggatc ggccagcagt tccgcccggt tccattcgga
     1501 aacggtccat ccgccccagc aggagatttc cggcaatgac gctcgcccct tcccgcaccg
     1561 gcctgatccg catcgccgtg gtggccgcgg cgctcgccct gtcggcctgc gccagcaatg
     1621 gcctgctcga tgaactggat cagacccagc ccgtagggtc ggatttctcc aaggaactct
     1681 tcaaggacta cgcctatctc gcccgttcgt tcggcgatgc cggcaattcc gcgagcacgg
     1741 ccttcgatgc cggcaattcc ctgtcgctct ccggcgacag cagcggcgag atcgccgatc
     1801 tggcgacagc ctacgcccaa aaagcgctgg acgccgcgca gggtcaggaa gtcctgcccg
     1861 aagccgcgcc ggacggcaac gcggatgccg atcagatgcg ccagcgcctg ctcgcaagcc
     1921 tggaaaatgg ccggagcaaa ttccccgcgg acgccgcgcg tgcgcaggtg caatacgatt
     1981 gctgggtgat gaatgctcgc gtggaaagcc tgcgcagcg
//
LOCUS       DASUJG010000262         3247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_444577, whole genome shotgun sequence.
ACCESSION   DASUJG010000262 DASUJG010000000
VERSION     DASUJG010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444577"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VFX38_05635"
     CDS             complement(<1..565)
                     /locus_tag="VFX38_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314370.1"
                     /translation="MLRKSACILVAASVLALGGCVSAGTVADKIVGAPNGGHPAAYPG
                     AMGKIARAFYSARYSIPVGPPPATLAFAIVEPRNYGFSAAEKPGKTKSTRVFEWQVAN
                     APAAAFADYHIDSMDEFAERISRGLARAPLCQPPAGTVILLPGWGEQKETLLGYALDF
                     ANHGYRVILVDLRGQGASSGDYVSFGLV"
     gene            complement(765..2060)
                     /locus_tag="VFX38_05640"
     CDS             complement(765..2060)
                     /locus_tag="VFX38_05640"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX5314371.1"
                     /translation="MSAPRRVAIVGGLRIPFCRAGSHYANASNKDMLTAVMKALVDRY
                     GLKGQRLGDVAAGATFKHPRDWNLAREALLDSGLARDTPAFDLQRACGTSLSACVLIA
                     HKIALGNIEAGIGAGVDSISDLPLEFTDRLRRTVLAANRAKTLGKRLGLFSHVRPGDL
                     KPQVPGVVEPHTRLNMGQSCERMARAWKVSRESQDKLALASHTNAARAWDDGFYRDLV
                     VPFAGAERDNNVRSDSSLEKLAKLRTSFAHDSQATLTAGNSSPLTDGAAGVLLASEDW
                     ARERGLPIQAWLMDSEVAAVNFIDGDVAGDGLLMAPTYAVSRMLARNKLVFDDFDFFE
                     IHEAFAAQVLCTLKAWEDPEFSRRRLDREPPLGEVDRAKLNIRGGSVALGHPFGATGA
                     RIVATLAKLLGDKGSGRGLISICTGGGMGVTAILQAAEN"
     gene            2196..>3247
                     /locus_tag="VFX38_05645"
     CDS             2196..>3247
                     /locus_tag="VFX38_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314372.1"
                     /translation="MNGRNRFFRSSRRLLGQGGLLIFGMALMAGTVASPAAAASGKAV
                     TYAPPDMSEVSAAELKNYYAWREHMDWLLAPIHTRADLEKYLKETEKSASPLDALAPD
                     ARRRLLSEIQFGPAGAFVVSYADLQYLSTEQVYKILALFGQQNWALRFTGGKIRAASP
                     QPKSSSTQESIVQRRFDEYWKATQSVGESSRKQAQLVGDKYDQLFSPLQNPARLRSLP
                     SGDLRLLLRAAFLAAGTTSNAKYAADAQMDFAEMERRHFAGWPDYDAMFQSLVLTRQF
                     AAARQFSLAHPDADSAPFPEYRDEAGNAGKNTPTVLAVSTTGNELVRRPVDLDRPALL
                     LILTDPHCYFCAQFEAA"
BASE COUNT          578 a         1058 c         1055 g          556 t
ORIGIN      
        1 cgacgagccc gaagcttacg taatcgccgc tcgatgcgcc ctggccgcgg agatcgacga
       61 ggatgacgcg gtagccgtga ttggcgaagt cgagcgcata gccgagaagc gtttctttct
      121 gctcgcccca gccgggaagc aggatgacgg tcccggcggg cggctggcac aggggcgcgc
      181 gtgcgaggcc gcgcgaaatg cgctccgcaa actcatccat gctgtcgatg tgataatcgg
      241 cgaatgctgc ggcgggagca ttggcgacct gccattcgaa tacccgcgtc gatttcgttt
      301 ttccgggctt ctcggcggcg ctgaagccat agttgcgcgg ttccacgatc gcgaaggcga
      361 gcgtcgccgg cggaggtccc acgggaatag aatagcgcgc ggaatagaag gcccgcgcga
      421 ttttccccat tgcgcccgga tatgcagcgg ggtgtcctcc attcggcgca ccgacgatct
      481 tgtcggcgac ggtgccggcg cttacgcaac cgccgagtgc gagcaccgaa gcggcaacga
      541 gaatgcacgc cgattttcgc aacatgcccg gcatcataaa cctcgaacgg ccggcatcgc
      601 cgctctccgt acttccccct tccgttagga agggggatcg agggggatgt cctggagtcg
      661 cgggaatcga agctgtcgca ggcccgcgca cgcgcagcgt tgcagggatc ccgtgagcgc
      721 tgcggaatta tcgccgcgac ccccgcgtcc tctgcgttcc atccttagtt ttcggcagct
      781 tggagaatcg ccgtgacgcc catgccgccg ccggtgcaga tcgagatcag gccgcgcccg
      841 cttcccttgt cgccgagaag tttcgcgagc gtcgcgacga tgcgcgcgcc ggtcgcgccg
      901 aagggatgac cgagggcgac gctgccaccc ctgatgttca gtttcgcgcg atccacttcg
      961 ccgagcggcg gttcgcgatc gaggcggcga cgtgagaact cgggatcctc ccaggctttg
     1021 agcgtacaca gcacctgcgc cgcgaaggct tcgtgaattt cgaagaagtc gaagtcgtcg
     1081 aagacgagct tattgcgcgc gagcatcctc gaaactgcat aggtcggcgc catcagcagg
     1141 ccgtcgcccg cgacatcgcc atcaatgaag ttcacggccg cgacctcgct gtccatgagc
     1201 caggcctgga tcggcaggcc gcgttcgcgc gcccagtctt cggatgcaag cagcacgcct
     1261 gccgcgccgt cggtgagcgg actcgaattg ccggccgtca aggtcgcctg cgaatcgtgc
     1321 gcgaaggaag tgcgcagctt cgcgagtttt tcgagcgagc tgtcgctgcg cacgttgttg
     1381 tcgcgctcgg cgccggcgaa cggcacgacg agatcgcgat agaaaccgtc gtcccaggcg
     1441 cgggcggcgt tcgtatggct cgcgagcgcg agtttgtcct gcgactcgcg gcttaccttc
     1501 caggcccgcg ccatccgctc gcaactctgg cccatgttga gccgcgtgtg cggttcgacg
     1561 acgcccggca cctgcggctt gaggtcgccc gggcgcacat gggaaaacag gccaaggcgc
     1621 tttcccaggg tcttcgcgcg attggcggcg agcaccgtgc ggcgcagccg atcggtgaat
     1681 tcgagcggca gatcgctgat cgaatccacg cccgcgccga tgccggcctc gatgttgccg
     1741 agcgcgatct tgtgcgcgat cagaacgcag gcggaaagac tcgtgccgca ggcgcgctgg
     1801 agatcgaagg cgggagtgtc gcgcgcgagc cccgaatcga gcaacgcctc gcgggcgaga
     1861 ttccagtcgc gcggatgctt gaaggtcgcg ccggccgcaa cgtcgccgag ccgctggccc
     1921 ttgagcccgt agcgatcgac cagcgccttc atcacggcgg tgagcatgtc cttgttgctc
     1981 gcgttcgcgt agtgcgagcc agcccggcag aagggaatgc gcaggccgcc cacgatcgcg
     2041 acccgtctcg gcgccgacac attcatgaca gactctcgcg gagtatccga tcctatcctg
     2101 ccacaacagg agcgggaaag cgacgcaatc ggacgccgtg cgcgccgccg aagcgtacaa
     2161 tcaaacccgt gggcgatgac ttgcgggatt cgatcatgaa cgggcgcaac cgattctttc
     2221 gttcatcgcg ccggcttctg ggccagggcg gcctgctgat attcggcatg gcgctgatgg
     2281 cggggacggt tgcgtcgccg gcggcagcgg cgagcgggaa ggccgtgacc tacgcgccgc
     2341 ccgacatgag cgaggtgagc gcggcggagc tgaagaatta ttacgcctgg cgcgaacaca
     2401 tggactggct gctcgcgccg atccacaccc gcgccgacct cgaaaaatat ctcaaggaga
     2461 ccgagaaatc cgcctctccg ctggatgcac tggcacccga tgcgcggcgc cggctgctgt
     2521 cggagatcca gttcggcccc gcgggcgcat tcgttgtctc ctacgcggac ctgcagtacc
     2581 tctcgaccga acaggtctac aaaatccttg cgttgttcgg ccagcagaac tgggcgctcc
     2641 ggttcaccgg cggaaagatt cgtgccgcga gcccgcagcc gaaatcgtct tcgacgcagg
     2701 aatcaatcgt gcaacggcgc ttcgacgagt attggaaagc gacgcaatcc gtcggcgaat
     2761 cctcgcggaa gcaggcccaa ctggtcggcg acaagtacga tcaactgttt tcgccgctgc
     2821 agaatcccgc ccgactacga agtctcccca gcggcgacct gcggctgctg cttcgtgcgg
     2881 cctttctggc ggcggggaca acgtccaatg cgaaatacgc ggccgatgcg cagatggatt
     2941 tcgcggaaat ggaacggcgc cacttcgcgg gatggccgga ctacgacgcc atgttccagt
     3001 cgctcgtgct cacgcgccaa ttcgctgccg cccgccaatt ctctctcgcg catcccgacg
     3061 cggactccgc gccctttccc gagtatcgcg acgaggccgg aaacgccggg aagaacacgc
     3121 cgacggtgct cgcggtgagc acaacgggaa acgaactcgt tcgccgccca gtcgacctcg
     3181 accggcccgc gcttctgttg attctcactg accctcattg ttatttttgc gcgcaattcg
     3241 aagcggc
//
LOCUS       DASUJG010000263         3671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1056961, whole genome shotgun sequence.
ACCESSION   DASUJG010000263 DASUJG010000000
VERSION     DASUJG010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056961"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..316
                     /locus_tag="VFX38_05650"
     CDS             41..316
                     /locus_tag="VFX38_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX5314373.1"
                     /translation="MVGTIRKPRLKRASLSEVKDDLSRYLREAEKHDILITRHGKPAG
                     VLIGFESEDDWFEYRLENDPRFLDRIESARRSLRTGRGVRLEEVEGG"
     gene            complement(329..819)
                     /locus_tag="VFX38_05655"
                     /pseudo
     CDS             complement(329..819)
                     /locus_tag="VFX38_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492227.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase"
     gene            complement(964..>3671)
                     /locus_tag="VFX38_05660"
     CDS             complement(964..>3671)
                     /locus_tag="VFX38_05660"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEX5314374.1"
                     /translation="TICKILVDPENPRNLYAASLGHVFVAGANRGVFKSADGGAHWTK
                     ILFVNDQTGVADLAMDPRNPEVLYAAAWQAYRTPWKLSSGGPGSGIYKTSDGGAHWTN
                     ISSALGLGSEPLGKIGVAVAPSSPNVVYAIVQAKDGGVFRSDDAGAHFTRVSNDLELR
                     QRAFYYMKIVVNPKDPDTVYVPNVNFYVSQDGGKNFKELDVPHGDHHTIWINPDDPNI
                     LLEGNDGGATVSTDGGKSWSSVHNQPTGQFYHVNLDDQFPFHVYGAQQDEGSFAGPSA
                     SPDGVIPLSAWQSVADGESTVVVPQPGTPNLTYGSGYYSIFVQYDLATGQYRSVTPWP
                     NYMDGAASNQLRYRFAWTHPILFSPADHKTLFVGSQYVQKSTDYGLTWKTISPDLTRN
                     SAATEGPTGGPIDLDQTGAEVYPWVSAIGISPLDGDVIWAGSSDGLVHVTTDGGAHWQ
                     AVRPPQLPAWALVDAIEPSHFAKGTAYLAARRYMWDDFRPYVYETTDYGRHWTAITNG
                     LPADEYVFDVVQDPNDAKLLFLAAKNTVYVSFDGGADWQPLTLNLPRVQVRGIAINSR
                     EGEVAIATHGRAFWILDDLALLEAMTGNPAVTKQAPALFTPGDAWLTYSYGGPTQPDG
                     VGANPPFGATVFFHIPENYDGKTAVTLSFADANGRLVRSFPLHAREKTTPAASAASAT
                     PAAERALDERRLTAIDPGMNRFQWNLRYPDAVGVTGFYPPEPAGGLAATLDGPQVLPG
                     SYRVTLDYGGDSVTRNFRVALDPRLHASQSDLEASLALQFKIQNALDTLDESLDKAIA
                     TRARLAAAVSAHRLSATQAAGASSVLGAAIASVVQLDIHSSEGDVMKPMKLHSHLAYL
                     GSGVGIAYVRPTAAQYAVFRYLRQQANDGEQRLAAAITAAEKLLP"
BASE COUNT          645 a         1135 c         1213 g          678 t
ORIGIN      
        1 ttgaaattct ggcgatcgtg gcgaaatcgg aggctgaatc atggttggca caattcggaa
       61 gccgcgactg aaaagggctt cgctttcgga agtgaaggac gatctttcgc ggtatctgcg
      121 cgaagcggag aaacacgaca tactcatcac ccgccacggc aagcctgccg gagtattgat
      181 cggtttcgaa tccgaagatg actggttcga gtaccggctg gagaacgatc cgcgttttct
      241 ggaccggatc gaatccgcgc ggagaagcct gcggacaggc cgaggcgtga gactggaaga
      301 ggtggaaggc ggctaaggca gccctgtttc atgtctcctt cttcaaatcc gggaactctt
      361 cttcgaaata ctccgctgtt acgcgaaggc ctcgcccacg gcgttcactt tcgaggttgc
      421 gcaaatccac gcggcggatt tttcccgaga tggttttcgg cagttcgctt acaaactcga
      481 tgcggcggat gcgcttgtag ggcgccagcc gctcgcgggc gaacgcgaga agggcgcagg
      541 caagttcggc gtccgcgatg ctgcccttcg tcagcgtgac gaaggctttg ggcacggcga
      601 aacgcagcgg atcggggctc ggcacgaccg cgacttcggt cacggacacg tgttcgatca
      661 gcgcgctttc gagttcgaag ggctgatgcg ataatccgag gccttgaaga cgtcgtcggc
      721 gcggccgacg aaggtgaggt aaccatcttc gtcgcgcagc gcgacgtcgc cggtgtgata
      781 gcagccgtcg cgcatgacca cagccatctt ttcggcggtc gttgcaggca ttcgtagaat
      841 tgccgggaac gctccgctga tcccggcctg cgaccgaatg gcttttcgcg tcgccgccgg
      901 gaatgatgcg ggtgtggcgc cggcgaaagc tgttcgcaaa tcagcggcgc ggcgtcgtaa
      961 ccatcagggc agcaacttct cggcggcggt gatcgcggcg gcgaggcgct gctctccgtc
     1021 gttcgcctgt tgtcgcaggt aacggaagac ggcgtactgc gccgcggtcg ggcgcacata
     1081 cgctatgccg actcccgagc cgagataggc gagatggctg tggagcttca tcggcttcat
     1141 cacgtcgcct tcgctcgaat ggatgtcgag ctgcacgacc gaggcgatgg cggcgccgag
     1201 caccgaggat gcgcccgccg cctgtgtcgc gctcaggcga tgcgcggaaa ccgcggcggc
     1261 aagtcgtgcg cgggtcgcga tcgccttgtc gagactctcg tcgagcgtgt cgagcgcgtt
     1321 ctgaatcttg aactgtagcg cgaggctcgc ctcgagatcg ctttggctcg catgcaagcg
     1381 cggatcgagc gcgacgcgga aattccgcgt gacgctgtcg ccgccgtaat cgagcgtcac
     1441 ccgatagctg ccgggaagca cctgcggccc gtcgagcgtc gcggcaaggc cgccggcggg
     1501 ctcgggcgga tagaagccgg tcactccgac ggcatcggga taccgaaggt tccactgaaa
     1561 gcgattcatg cccggatcaa tcgccgtgag acggcgctcg tcgagcgccc gttcggctgc
     1621 gggcgtcgca cttgccgcgc tcgcggccgg cgtcgtcttc tcgcgcgcat gcaacgggaa
     1681 gctgcgaacg agtcggccgt tcgcatcggc gaagctcagc gtcaccgcag tcttgccgtc
     1741 gtaattctcg ggaatatgaa agaacacggt cgcgccgaag ggcggattcg caccgacgcc
     1801 gtcgggctgc gtggggccgc cataggaata cgtgagccag gcatcgccgg gcgtgaaaag
     1861 tgccggcgcc tgcttcgtga ccgcgggatt gcccgtcatc gcttcgagca gcgcgagatc
     1921 gtcgagaatc cagaacgcgc gcccgtgcgt cgcgatcgcg acctcgccct cgcggctgtt
     1981 gatcgcgatg ccgcgtacct gcacccgcgg gagattgagc gtcagcggct gccagtcggc
     2041 gccgccgtcg aaactcacgt acaccgtatt cttcgcggca agaaacaaaa gtttcgcatc
     2101 gttcggatcc tgcacgacgt cgaagacgta ctcgtcggcc ggcaatccgt tcgtgatcgc
     2161 ggtccagtgg cggccgtaat cggtggtttc gtaaacatac ggccggaagt cgtcccacat
     2221 gtagcggcgc gccgcgagat acgccgtgcc cttcgcgaaa tgcgagggct cgatcgcatc
     2281 caccagcgcc cacgccggca attgcggagg ccgcaccgcc tgccagtgcg cgccgccgtc
     2341 ggtcgtgaca tggacgaggc cgtcgctcga tcccgcccag atcacgtcgc cgtcgagcgg
     2401 cgaaatcccg atcgccgaaa cccagggata gacttccgca ccggtctgat cgagatcgat
     2461 cggcccgccg gtcggtcctt cggtcgccgc cgagttgcgc gtgaggtccg ggctgatcgt
     2521 cttccacgta agcccgtaat ccgtgctctt ctgcacgtat tgggagccga cgaagagcgt
     2581 cttgtgatcc gccggcgaga acaggatcgg atgcgtccac gcgaagcggt aacgcaactg
     2641 attcgatgcc gcgccatcca tgtagttcgg ccagggcgtc acgctgcggt actgcccggt
     2701 cgcgagatcg tactgcacga agatgctgta gtagccgctg ccgtaggtga ggttcggcgt
     2761 ccccggctgc ggcacgacta ccgtgctctc gccatcggcg accgactgcc aggcggaaag
     2821 cggaatcacg ccgtcggggc tcgcgctcgg acctgcgaag gagccctcgt cctgctgcgc
     2881 gccatagacg tggaacggga actggtcgtc gaggttcaca tggtagaact ggccggtcgg
     2941 ctggttgtgc acgctcgacc agcttttgcc gccgtcggtc gaaaccgtgg caccgccgtc
     3001 gttgccttcg agcaggatgt tcgggtcgtc gggattgatc cagatcgtgt gatggtcgcc
     3061 gtgcggcacg tcgagttcct tgaagttctt gccgccgtcc tgcgagacgt aaaagttcac
     3121 gttcggaaca tagaccgtgt ccggatcctt cgggttcacg acgatcttca tgtagtagaa
     3181 ggcgcgctgg cgaagctcca gatcgttcga cacgcgcgtg aagtgcgctc ccgcatcgtc
     3241 ggagcggaac acgccgccgt ccttcgcctg cacgatcgca tagacgacat tcggcgaact
     3301 cggggcgacg gcgacgccga tctttccgag cggctcgctt ccaaggccga gcgcggagct
     3361 gatgttcgtc cagtgcgcgc cgccatcgct cgtcttgtaa atgccgcttc ccggcccgcc
     3421 gctcgaaagc ttccagggcg tgcggtacgc ctgccaggcc gcggcgtaca gcacttcggg
     3481 attgcgcgga tccatcgcga gatccgcgac gcccgtttga tcgttcacga agagaatctt
     3541 cgtccagtgc gcaccaccgt cggcggactt gaagacgccg cgattcgcgc cggcgacgaa
     3601 cacgtgaccg agcgaggccg catacaggtt tcgcggattt tcgggatcga cgaggatctt
     3661 gcagatcgta t
//
LOCUS       DASUJG010000264         2126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_567043, whole genome shotgun sequence.
ACCESSION   DASUJG010000264 DASUJG010000000
VERSION     DASUJG010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567043"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VFX38_05665"
     CDS             complement(<1..182)
                     /locus_tag="VFX38_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEX5314375.1"
                     /translation="MSKDGAIERLRRYRSFGGTVEFYRHASHACAAPMRFSVYRPPQA
                     ATQAVPVIYWLSGLTCT"
     gene            complement(179..1291)
                     /locus_tag="VFX38_05670"
     CDS             complement(179..1291)
                     /locus_tag="VFX38_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015005268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEX5314376.1"
                     /translation="MKTRAAVAWEAGRPLEIEEVELAGPRAGEVLLRNVATGVCHTDA
                     FTLSGADPEGLFPTILGHEGGAVVEEIGADVKSVAPGDHVIPLYTPECGQCEYCRSGK
                     TNLCQAIRATQGRGLMPDGTSRFSRNGKPIFHYMGTSTFSEYTVLPEISIAKINREAP
                     LEKVCLLACGVTTGIGAVLNTAKVEAGATVAVFGLGGIGLAVIQGAVLAKAGRIVAID
                     VNEDKFEFARQLGATDTVNPAKHDRPIQEVLVELTKGGVDYSFECVGKVALMRAALEC
                     CHKGWGVSTIIGVAGAGQEIATRPFQLVTGRVWKGTAFGGVKGRSELPEYVEWYLAGR
                     IELDRMITHTMALEDINRAFELMHAGESIRSVIHYR"
     gene            1366..>2126
                     /locus_tag="VFX38_05675"
     CDS             1366..>2126
                     /locus_tag="VFX38_05675"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5314377.1"
                     /translation="MLISLREITVRFSAEPVLDAVNLSIQEGERLCLVGRNGAGKSTL
                     MHVIAGSLEADSGEREHADTLGIAALEQDTPAGLAGPVYDIVTRGLGETGRRLLAFSR
                     LAGSGGESARMLAVQHQLEESGAWNEKLRADAIISRMKLDPEAEFRALSGGLQRRVML
                     AAALVSDPDLLLLDEPTNHLDIEGIRWLEDLLVGARSTLVFISHDRAFLDRVATRIVE
                     VDRGRLHNWPGNYAEYLWRKRAALAAEATANREFD"
BASE COUNT          352 a          720 c          674 g          380 t
ORIGIN      
        1 gtgcaagtaa gcccggacaa ccagtagatg acgggaaccg cctgcgttgc ggcctgcggc
       61 ggacgataga cggaaaatcg catcggcgcc gcgcaggcgt gggaagcgtg ccgataaaac
      121 tcgacggttc cgccgaagga ccgatagcgg cgcaggcgtt cgatcgcgcc gtctttgctc
      181 atcggtaatg gatcacggag cggatgcttt ctcccgcgtg catgagttcg aaggcccggt
      241 tgatgtcttc gagcgccatc gtgtgcgtga tcatgcgatc gagttcgatc cggccggcga
      301 gataccattc gacgtattcg ggtagctccg aacggccctt gacgccgccg aaggccgtcc
      361 ccttccatac gcgtcctgtc accaattgga acgggcgcgt cgcgatttcc tgcccggcgc
      421 cggcgacgcc gatgatggtg gaaacgcccc agcccttgtg acagcattcg agcgcggcac
      481 gcatgagcgc aaccttgccg acgcactcga aggagtaatc caccccgccc ttcgtcagtt
      541 ccacgaggac ttcctggatc gggcggtcgt gtttcgcggg attcacggtg tcggtcgcgc
      601 cgagttggcg cgcgaactcg aacttgtctt cgttcacgtc gatcgcgacg atgcgccccg
      661 ccttcgcgag caccgcgccc tggatcacgg caagcccgat cccgccgaga ccgaagacgg
      721 cgaccgtcgc ccccgcctcc actttcgcgg tgttgagcac cgcaccgatg ccggtcgtga
      781 ctccgcaggc gagcagacag actttctcca gcggcgcctc gcgattgatc ttcgcgatcg
      841 agatctcggg gagcaccgtg tactcggaaa aggtgctcgt gcccatgtaa tgaaagatcg
      901 gtttgccgtt ccgcgaaaag cgcgaggttc cgtcgggcat caggccacgg ccctgggtcg
      961 cgcggatcgc ctggcacaaa ttggtcttgc cggagcggca gtattcgcat tggccgcatt
     1021 cgggcgtgta gagcggaatc acgtgatctc ccggcgcgac gctcttcaca tcggcgccga
     1081 tttcttcgac gacggccccg ccttcgtgcc cgaggatcgt cgggaacagt ccctccgggt
     1141 ccgcgcccga aagcgtgaag gcgtcggtgt gacagacgcc ggtcgcgacg ttgcgcagca
     1201 gcacctcgcc cgcccgcggg cccgcgagtt cgacttcctc gatttccagg ggccggcccg
     1261 cttcccaggc gacggccgcg cgcgttttca tgctgcgttc cttccgacgg agttctctgc
     1321 gattatagtc ggcatggatc cggtcctccc cttcccgctt cgcccatgtt gatcagtctg
     1381 cgcgagatca ccgtgcgttt cagcgccgag cccgtgctcg acgcggtcaa cctgagcatt
     1441 caggaaggcg aacggctctg cctcgtcggg cgcaacggcg cgggaaaatc cacgctgatg
     1501 catgtcatcg ccggcagcct cgaagccgac tccggcgagc gcgaacacgc cgacacactc
     1561 ggcatcgccg cgctcgaaca ggacacgccc gccggtcttg ccggtcccgt ctacgacatc
     1621 gtcacccgcg gactcggcga aaccgggcgc cggctgctcg ccttctcgcg cctcgccggc
     1681 tcgggcgggg aatctgcgcg gatgctagcc gtgcagcacc aactggaaga atccggcgcc
     1741 tggaacgaga agttgcgcgc cgatgcgatc atctcgcgca tgaagctcga ccccgaggcg
     1801 gagttccgcg cgctttccgg cggattgcag cgccgcgtga tgctcgccgc ggctctcgtg
     1861 agcgatcccg atctgctgct gctcgacgag ccgaccaatc atctggacat cgaaggcatc
     1921 cgctggctgg aggatctgct cgtcggcgcc cgttcgacgc tcgtcttcat cagccatgac
     1981 cgcgcctttc tcgatcgcgt cgcgacgcgc atcgtcgagg tggatcgcgg acggctccac
     2041 aactggcccg ggaactacgc ggaatatctg tggcgcaagc gcgccgcgct cgcggcggag
     2101 gccacggcga atcgcgaatt cgacaa
//
LOCUS       DASUJG010000265         5374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_79232, whole genome shotgun sequence.
ACCESSION   DASUJG010000265 DASUJG010000000
VERSION     DASUJG010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79232"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VFX38_05680"
     CDS             complement(<1..584)
                     /locus_tag="VFX38_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEX5314378.1"
                     /translation="MAQTREREPASEIAAHPSLRINFLGLEFATPLVLLSGCVGFGHE
                     YPRVAGFSHRDIGAVCLKGTTLAARLGNPAHRLWETPMGMLNAIGLQNPGARAVVRDI
                     LPTLDFAETRYIANVSGATIEEYAQVCRIFDDSPIDALEINISCPNVKEGGVIFGNSP
                     AMSARVVAACRAVTKKPLITKLSPNQTDIAENARL"
     gene            complement(599..2521)
                     /gene="rpoD"
                     /locus_tag="VFX38_05685"
                     /pseudo
     CDS             complement(599..2521)
                     /gene="rpoD"
                     /locus_tag="VFX38_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932930.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
     gene            complement(2721..4460)
                     /gene="dnaG"
                     /locus_tag="VFX38_05690"
     CDS             complement(2721..4460)
                     /gene="dnaG"
                     /locus_tag="VFX38_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018865575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX5314379.1"
                     /translation="MAGLIPQSFIDELIARADIVELVGRRLRLKRQGREFSALCPFHE
                     EKSPSFTVSPQKQFFHCFGCGAHGTALGFLMRYENLDFVQAVEHLAADLGLEVPHEGG
                     AKTRPHTPLYEALGRAQAFFGTELSRSARAQEYLRRRGLTAPAVETWGIGYAPTDGTA
                     LLAALRGAGVDEAVLVAAGLIARNTRGSYDRFRDRLMLPIRDSRGRVTGFGGRTIGEG
                     KPKYLNSPETPVFHKGRMLYGLYEARRQQAGIERLVVVEGYLDVIALAGAGFRGAVAT
                     LGTAVSEAQMELLFRSGADEVIFCFDGDAAGERAAWRALEQSLPAVAPGRVLRFGFLP
                     AGEDPDSLVRHDGLEAFEKILTAAQPLSVFLLDRLAAGGLDGAEGRARFAGALRPLLG
                     RVRDELFRDALLTEAAGRLQMAPERLTRLLLPPRRPAIAAASTSAGFRPAISIDPRVA
                     RALSLALALPGRVASEGWPESIAALALPGAALLAEVVETLRTDPHLTTAGVLEHWRDR
                     PEASLLGRLASRDPLIDGDEALAAEYRKVLADLVRTGREAEIRQLREAATLRDLDQTE
                     RERLRRLLGKEEA"
     gene            complement(4552..5004)
                     /locus_tag="VFX38_05695"
     CDS             complement(4552..5004)
                     /locus_tag="VFX38_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX5314380.1"
                     /translation="MDVKSHLETDLKAALKARDVARVSAIRLALAAVKQREIDSGHRE
                     GLDQAEALGVIERLIKQRHEAAVQYRAAGRDASAAGEEAEAKLLSAYLPEPIDSEALD
                     AEIRSVIREAGAGGRRDMGKVMGLLKARLAGRADMAEVSRRVREQLSH"
     gene            complement(5059..5274)
                     /gene="rpsU"
                     /locus_tag="VFX38_05700"
     CDS             complement(5059..5274)
                     /gene="rpsU"
                     /locus_tag="VFX38_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX5314381.1"
                     /translation="MPSVRVRDHEQFDIALRRFKRACEKAGVLTELRRREYYEKPTQE
                     RKRKRAAAVKRHAKKISREHSRRQRLY"
BASE COUNT          847 a         1738 c         1742 g         1047 t
ORIGIN      
        1 aggcgggcat tctcggcgat gtcggtctgg ttgggcgaga gtttggtaat cagaggcttt
       61 ttcgtcaccg cccggcaggc ggcgacgacg cgcgccgaca tcgccggcga attgccgaaa
      121 atgacgcctc cctccttcac gttcgggcag gaaatgttga tctcgagcgc gtcgattggc
      181 gaatcgtcga agatgcggca gacctgcgca tattcctcga tcgtcgcgcc ggagacattg
      241 gcgatgtagc gggtttcggc aaagtcgagc gtgggcagga tgtcgcgcac cacggcacgc
      301 gcgccgggat tctgcaggcc gatggcgttg agcattccca ttggcgtttc ccacagccga
      361 tgcgccggat tgccgagccg cgcggcgagc gtcgtaccct tgagacatac ggcaccgatg
      421 tcgcgatggg aaaatccggc gacgcgcggg tattcgtggc cgaagccgac gcagcccgag
      481 agcaacacca gcggcgtcgc gaactcgaga ccgagaaaat tgatccgaag cgacgggtgc
      541 gcagcaattt ccgacgccgg ctcccgctcc ctcgtctgcg ccattacggg tctgccgttc
      601 agggcgactc ttcgtcgaga aaactcttca atcgctccga gcgcgacgga tgcttcagtt
      661 tgcggatggc tttcgcctcg atctggcgaa tgcgctcgcg ggtcacgtcg aactgctttc
      721 cgacttcctc cagcgtgtgg tcggtattgg tgctgatgcc gaagcgcatc cgcagcacct
      781 tcgcctcgcg cgcggtaagc gttgcgagca ccgagtgcat cgcctcgcgc aacccctcgt
      841 tgaccgcgga gtcgatcggc gccgtcacgg aattgtcctc gatgaagtcg ccgatatgcg
      901 agtcctcgtc gtcgccgatc ggagtttcca tggagatcgg ctccttggaa atccgcatga
      961 ccttgcgcac cttgtcttcc ggcatctcca tccgctgcgc gagttcctcg ggcgtcggat
     1021 cccggcccat ttcctgcagg agctggcgct gaatgcggtt gagcttgttg atggtctcga
     1081 tcatgtgcac cggaatgcgg atggtgcgcg cctgatcggc gatcgagcga gtaatcgcct
     1141 ggcggatcca ccaggtcgcg taggtgctga atttgtatcc gcggcggtat tcgaacttgt
     1201 ccaccgcttt catgaggccg atgttgcctt cctgaatgag atcgaggaac tggaggccgc
     1261 gattcgtgta cttcttggcg atcgaaatca cgagacggag gttcgcctcc accatctcgc
     1321 gcttggcacg ccgcgccttg gcatcgccga tcgagatttg tcgcttgagt tcgcgaattt
     1381 ccgcgagcgg catcttgaat tggtgttcca tcctcacgag atcctgctgc agacgataca
     1441 cgtcttcgct gcgcgcgcga atcttcgagg accacttgcg cttggcgcgg atctgatgct
     1501 ccacccattt gcggtcggtt tcatgttccg gaaagccgcg cacgaagtcc ttgcgcggca
     1561 tgccggcctc gttgacgcag atcttcatga tctcgtgctc gcgatcgcgg atgaagtcga
     1621 aatgggtctt catgttcgcg taaagaagtt cgtgcatccg cggcgtgagc ttcagctcct
     1681 gcatcaagcg cgtcgtctcg ctgcggttgc gctccgcgct cttcgcccgg tagccgtagc
     1741 gcgcgatcga gcgcacggtc ttcagatgct gcccgcgaat cgcttccata tgcgccgcga
     1801 ggcgtttgag atcgagacct tcggcaggat tggtcgagcg gcgctgcttg ggtggcggag
     1861 gggtcggccc ggatttctct tcctcgattt cctcttccac ttcttcgttg ctgtcgctgc
     1921 cgtcttcctc gtactcgacg tcgttggggc tgagcgcggc actctcctct gccgcttcct
     1981 cgtcctccgc ctcgtgctcg gcgatgatga actcgttgaa gccggtcacg acctcggtca
     2041 cccgctgttc tccggcgcgc gccttgtcgt actcggacag caggagcgcg actgactcgg
     2101 agacgccgct gatggcctgg aacatctgat cgagccctgc ctcgatgcgt ttggcgatgt
     2161 gaatttcgcc ctcgcgggtg aggaggtcca cctgccccat ttcgcgcatg tacatgcgca
     2221 ccgggtcggt cgtgcgcccg agttcggcgt ttaccgcggc gagcgccgcc gccgcttctt
     2281 cggccgcgtc ctcgtcggtg acctgctcgc cggcgagcat gagcatgtcg gcgtccggag
     2341 tgatctcgaa cacggtgatg cccatgttgt tgatcatgtc cacgatgtct tcgatctgct
     2401 cgggatccac gacctcctcg ggtagatggt cgttgatgtc ggcataggtc aggtagccct
     2461 gctccttgcc caaggcaatc aaattgcgaa cctgatcgtt gcgttcttcg ctcatgtcgt
     2521 tatccttgcg ccggtgacgc ggcgaacgtt acattttatc agccaaacca gtttttttga
     2581 gccccgactc cctgccgagg ttcccgagcg cggtcgccgg tgcgagcccg cagcgctcct
     2641 aacccattga ttaatatgtc taccagcgcc cgaaatccca tcccggcggc gaaaccgtga
     2701 ttgcgacggc ggtgctctgc tcaggcctcc tctttgccga ggagacggcg caggcgttcg
     2761 cgctcggtct gatccaggtc gcgcagcgtc gccgcctccc gcaattgtcg aatctccgct
     2821 tctctgccgg ttctgaccag gtcggccagt accttgcggt actccgccgc cagcgcctcg
     2881 tcgccatcga tcagaggatc gcggctcgcg aggcgcccca ggagcgaggc ctcggggcgg
     2941 tcgcgccagt gctccagcac tccggccgta gttagatggg ggtccgtgcg cagggtttca
     3001 acgacttcgg cgagcagggc ggctccgggc agcgcgagcg ccgcgatcga ttcgggccag
     3061 ccctcgctcg ccacgcggcc gggcagggcg agtgcgagac tcaaggcccg cgccacccgc
     3121 ggatcgatcg aaatcgccgg gcgaaagccg gcggatgtcg aggcggcggc gattgccggt
     3181 cggcgaggtg gcagcaacag gcgagtcagt cgttccggag ccatttgcag ccgtcccgcc
     3241 gcttcggtca gaagagcatc ccggaagagt tcgtcgcgca cacgcccgag cagcggacgc
     3301 agggcgccgg cgaagcgggc tcgtccctcg gccccatcga gacccccggc ggcgagccgg
     3361 tcaagaagaa acacggagag cggctgcgcg gccgtgagaa ttttctcgaa ggcttccaga
     3421 ccgtcatgtc gaacgagact gtccgggtcc tcgcctgccg gcagaaaacc gaagcgcagc
     3481 acgcgtccgg gagcaacggc cgggaggctt tgttcaagcg cccgccaggc ggcacgttcg
     3541 ccggcggcgt cgccgtcgaa gcagaagatc acctcgtcag cgccgctgcg aaacaacaac
     3601 tccatctgcg cctcgctcac ggccgttccg agtgttgcca ctgccccgcg aaagcccgcg
     3661 ccggcaaggg cgatcacgtc gaggtatccc tcgaccacga ccagccgctc gattccggct
     3721 tgttgccggc gagcctcgta gagcccgtag agcatccggc ccttgtgaaa cacgggagtt
     3781 tccggggaat tgagatattt gggcttgccc tcgccgatcg tccgccctcc gaaaccggtg
     3841 acacgacccc gcgaatcgcg gatgggaagc atcaggcgat cgcggaagcg atcataggaa
     3901 cctcgggtgt tgcgggcaat gagcccggcc gcgacgagca cagcctcatc gactcccgcc
     3961 ccacgcagcg ctgcgagcag ggccgtgccg tcggttggcg cgtagccgat tccccaggtt
     4021 tcgacggcgg gcgcggtgag tcctcgccgc cgaagatatt cttgcgcgcg cgcggagcgc
     4081 gacagctccg tgccgaagaa cgcctgcgca cgcccgagcg cttcgtagag cggggtgtgc
     4141 ggacgagtct tcgcgcctcc ctcgtgcggc acctcgaggc cgagatcggc ggcgaggtgc
     4201 tcgaccgcct gcacgaaatc cagattttcg tagcgcatca ggaatccgag cgcagtgccg
     4261 tgggccccgc agccgaaaca gtgaaagaac tgtttctgcg ggctcacggt gaaggagggg
     4321 cttttttcct cgtggaacgg acagagtgcc gagaattccc ggccctggcg cttcagacgc
     4381 aggcgccgcc ccaccagttc gacgatgtcc gcgcgagcaa tcagctcgtc gatgaaactc
     4441 tgcggaatca ggcccgccat gggtgcgcct ggtcgggttc gggatgccgc acagcgtagc
     4501 gcagtgtcgg gcgggattcg cgccgaccct caaggccgcc cgatgacggc ctcaatggga
     4561 cagttgttcg cgcacccgcc ggctgacttc ggccatgtcg gcgcgcccgg ccagcctcgc
     4621 cttgagaagc cccatgacct tgcccatgtc gcgccggcca ccggcgcctg cctcgcgtat
     4681 gacgctgcgg atttcggcgt cgagcgcctc cgaatcgatc ggttccggga gataggccga
     4741 aagcagcttc gcctcggctt cctcgccggc ggcgctcgcg tcgcggccgg ccgcgcggta
     4801 ctggaccgcg gcctcgtggc gctgcttgat cagtcgctcg atcaccccca gggcttcggc
     4861 ctggtcgagg ccttcgcggt gtccgctgtc aatctcgcgt tgcttgacgg cggccagcgc
     4921 gaggcggatg gcgctgaccc gcgcgacatc acgggccttg agcgcggcct tgaggtccgt
     4981 ctcgagatgg gatttcacgt ccatgcggcg atttcgcggc gatgcgacgg cagccgttgc
     5041 cgcaacggcg tcactccgtc agtacaggcg ctggcggcgg gaatgctcgc gggagatttt
     5101 cttggcatgg cgcttgacgg cggcggcgcg cttgcgcttt cgctcctgcg tcggcttttc
     5161 gtaatactcg cggcggcgca gttcggtgag cacaccggcc ttctcgcagg cacgcttgaa
     5221 gcggcgaagc gcgatgtcga actgttcatg gtcacggaca cggacggagg gcatacaggg
     5281 cgaggacccg ggcgaggaac tttacatttt aggatgccgc ggcctccggc gcaagttttt
     5341 cctgcggccc ggcgagtgcg agcagccttg cgag
//
LOCUS       DASUJG010000266         4471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_690701, whole genome shotgun sequence.
ACCESSION   DASUJG010000266 DASUJG010000000
VERSION     DASUJG010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690701"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VFX38_05705"
     CDS             complement(<1..464)
                     /locus_tag="VFX38_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEX5314382.1"
                     /translation="MSREIAVTMSASDPDDAYPAQAENESEERDRDRRDAEGRDAQQD
                     RGDAQDESSFDYDGDAAPGDEELPSREAQRTPNSADLDAINGYLCKIGFHSLLNAEEE
                     KEISGRARRGDEAARQRMIESNLRLVVSIARSYTGRGVPLLDLIEEGNLGLMV"
     gene            complement(486..1325)
                     /locus_tag="VFX38_05710"
     CDS             complement(486..1325)
                     /locus_tag="VFX38_05710"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX5314383.1"
                     /translation="MRPHSSRSLAIAAAMAAAVSLAACSGALNLPEQHRPQTYVVHRG
                     DTLYSIAWHYGLDFHQVARWNGISPPYRIYPNQRIKLYSSAPAHSRSASHHTQKKKAV
                     AANKTPRPAPKQTETVTAPAPQPAMPAGHAIAPGAGVAAAGGAPAGAAAPAAGPIHWQ
                     WPAGGTVEATFGRQDLSGKGIVITGDQGEPVLAAASGVVVYSGDALVGYGNLVIVKHN
                     DEWLSAYGQNQQLFVHEGEAVATGQKIATMGLGPNGRAELYFEIRENGDPVDPLQFLP
                     KTR"
     gene            complement(1322..1960)
                     /locus_tag="VFX38_05715"
     CDS             complement(1322..1960)
                     /locus_tag="VFX38_05715"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013820596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX5314384.1"
                     /translation="MTSSRTRERLIERLREEGISDVRVLAAMRAVPRHLFVEEALASR
                     AYEDMPLPIGHGQTISQPYIVALMTAALVAGEPPKRVLEIGTGCGYQTAVLARLVAEV
                     WSVERIRALLGRARKTLLELGIRNVNLRYGDGGEGWAECAPFDAILVTAAPEEVPPAL
                     LAQLAPGGRLIVPIGTRGAQRLMLYRRGESGIESEPMGAVSFVPFAPGVTAR"
     gene            complement(1981..2736)
                     /gene="surE"
                     /locus_tag="VFX38_05720"
     CDS             complement(1981..2736)
                     /gene="surE"
                     /locus_tag="VFX38_05720"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX5314385.1"
                     /translation="MLSILLSNDDGVHAPGLAALAASLAPLGEVHVVAPDRNRSAASN
                     SLTLDTPLRANRLDNGFVAVNGTPTDCVHLAITGLLDPPPDIVVAGINCGPNLGDDVL
                     SSGTVAAAMEGRFLGRPAIAVSLAGAGPCHYETAARVARLLVERLEEMQLPRETLLNV
                     NVPDRSWAQLAGLRATRLGARHRANPTVRAADPRGLPVYWVGEPGAAADAADGTDFAA
                     IAAGAVSVTPLTVNLTRRDALAGVEDWLRELAP"
     gene            2807..3382
                     /locus_tag="VFX38_05725"
     CDS             2807..3382
                     /locus_tag="VFX38_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEX5314386.1"
                     /translation="MSAEENERRLFREAVANATPIKRRAPLPRPPRPKAEARFRLAAE
                     REALAESRDDPRDPDEFGAEPDLAYAAPGVQHRVLRRLRRGQIPEQGLLDLHGKTQAA
                     AHEELAEFLTAAGVRGLQCVRIVHGKGHRSGPAGPVLKRAVARWLARRNDVLAYTSAR
                     PVDGGTGAVYVLLRRRAARASAIQDDARTRS"
     gene            complement(3406..4296)
                     /locus_tag="VFX38_05730"
     CDS             complement(3406..4296)
                     /locus_tag="VFX38_05730"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HEX5314387.1"
                     /translation="MGALRFRVADGPFLDGSDALAAPPVVSLRELQAASLKFEAELEG
                     GKEREESRWLTQLFAPGSSLGGARPKASVRDGNGALCVAKFPSRSDTRDMGAWELLAH
                     RLAAKAGIRVPPVRPLRLGSSPYTTFLAQRFDRTPRGRRRAFVSAMTLTQREDGEAGA
                     SYLELVDLLQSRGADTRADCEELFRRVVFSILIHNTDDHLRNHGFLLVPQGFRLSPAY
                     DINPMPERDELTLAINETDTHCDVDIAMRACRDYGLSDAVALAIWKQLRQAVSGWRRE
                     AAALKIPKSEQEQMAAAFAG"
BASE COUNT          753 a         1562 c         1433 g          723 t
ORIGIN      
        1 accatgagcc cgagattgcc ctcctcgatc agatcgagaa gcggcacgcc gcggccggtg
       61 tagctgcggg cgatgctcac gacgagacgc agattcgatt cgatcatgcg ctggcgcgcg
      121 gcctcgtcgc cgcggcgcgc gcgcccggaa atttccttct cttcttccgc gttcagaagc
      181 gaatgaaagc cgattttgca gagatagccg ttgatcgcat cgaggtcggc actgttcggc
      241 gtacgctgcg cctcgcgcga tggaagctct tcgtcgccgg gcgcggcgtc accgtcgtaa
      301 tcgaaggaag attcgtcctg cgcatcgccc ctgtcttgtt gcgcgtcgcg accttcggca
      361 tcgcggcgat cgcgatctcg ttcttcggat tcgttctccg cttgcgcggg atacgcatcg
      421 tccggatcag aagcactcat cgtgaccgcg atctccctac tcacccgacc ttgtctggga
      481 tgatgctacc gcgtcttggg aagaaactgc aacgggtcca ccggatcgcc gttctcgcga
      541 atctcgaaat acaactccgc gcgaccgttc ggaccgagac ccatcgtcgc gattttctga
      601 cccgtcgcca ccgcttcgcc ttcgtgcacg aacagttgct gattctggcc gtaggcactc
      661 aaccactcgt cgttgtgctt gacgatcacg agatttccgt aaccgacgag tgcatcgccg
      721 ctgtagacga cgacgcccga agcggccgca agcaccggct ctccctgatc gcccgtgatc
      781 acgatgccct tgccgctcag atcctggcgc ccgaaggtcg cctccacggt tccgccggcc
      841 ggccattgcc agtgaatcgg ccccgccgcg ggcgccgctg cgccggcggg agcgccgccc
      901 gcagccgcaa cgcccgcgcc cggcgcaatt gcgtgtccgg cgggcatcgc cggctgcggc
      961 gccggcgcgg tgacggtttc ggtctgcttc ggcgccggcc gcggagtttt gttcgcggcg
     1021 acggccttct tcttctgcgt gtgatgcgaa gcgctgcgcg aatgcgccgg cgccgacgaa
     1081 tacaacttga tacgttggtt gggataaatc cggtagggcg gcgaaatccc attccagcgc
     1141 gcgacttggt ggaagtcgag accgtaatgc caggcgatcg aatacagggt gtcgccgcga
     1201 tgcacgacat acgtctgcgg gcgatgctgc tcgggaagat tgagtgcgcc cgaacaagcg
     1261 gcgagcgaca cggcggccgc catcgcggcg gcaatcgcga gcgagcgcga cgaatgcggc
     1321 ctcacctcgc cgtcactccg ggcgcgaacg gcacgaaact caccgctccc atgggttcgg
     1381 attctatccc gctttcgcct cgccggtaca gcatcaggcg ttgcgcgccg cgcgtgccga
     1441 tcggcacgat gaggcgtccg ccgggagcga gttgcgcgag aagcgccggg ggcacctctt
     1501 ccggagccgc cgtcaccaga atagcgtcga agggcgcgca ttccgcccag ccttcgccac
     1561 cgtcaccgta gcgcagattg acgttgcgga tgccgagttc gagcaaggtc ttgcgggcac
     1621 gcccgaggag cgcgcgaatc cgctcgacgc tccacacttc ggcgacgagg cgcgcgagca
     1681 ccgcggtctg gtagccgcaa ccggttccga tttcgagcac gcgcttcggc ggctcgccgg
     1741 cgacgagcgc cgccgtcatg agcgcgacga tgtagggttg cgaaatcgtc tgcccgtgac
     1801 caatcggaag cggcatgtct tcgtaggcgc ggctcgcgag cgcctcctcg acgaacagat
     1861 ggcgcggcac ggcgcgcatc gcggccagca cccgcacgtc cgagatgccc tcctcgcgca
     1921 accgttcgat cagccgctcg cgcgtgcgcg aggacgtcat gccgatgccc tgcgccgcga
     1981 tcacggcgca agctcgcgca gccaatcctc gacgcccgcg agcgcatcgc ggcgggtgag
     2041 attcaccgtg agcggcgtaa ccgaaacggc gcccgcggcg atcgccgcga agtcggtccc
     2101 gtccgcggca tcggcggcgg cgcccggttc gcccacccag tacaccggca gcccgcgggg
     2161 atcggcagcg cgcactgtcg gattcgcacg atgccgcgcg ccgagtcgcg tcgcacgcag
     2221 gcccgcgagc tgcgcccagg agcgatccgg cacgttgacg ttcaaaagag tttcgcgcgg
     2281 caactgcatt tcctccaggc gctccaccag caggcgcgcg actctcgcgg ccgtctcgta
     2341 gtgacagggc cccgcgcccg cgagcgacac cgcgatcgcg ggacgcccga gaaagcgccc
     2401 ctccatcgcc gcggcgacgg tgcccgaaga gagcacgtcg tcgccgagat tcggtccgca
     2461 gttgatgccc gccacgacga tgtccggcgg gggatcgaga aggcccgtga tcgccagatg
     2521 cacgcaatcg gtcggcgtgc cgttgacggc gacgaatccg ttgtcgagtc ggttcgcgcg
     2581 caagggagta tcgagcgtga gcgaattgct ggccgcgctg cgatttcgat cgggcgcgac
     2641 cacgtgcacc tcgcccagcg gtgcgagcga ggcggcgagc gcggcaaggc ccggcgcatg
     2701 cacgccgtcg tcgttgctga gcaggatgga aagcactcga atctgcgccg atcgttcgtt
     2761 cgcgttaggc taatgatact caaccctccg ccggaagccg cgagccatga gcgccgagga
     2821 gaacgaacgg cgattattcc gcgaggcggt cgcgaacgcg acgccgatca aacgccgcgc
     2881 gccgctgccg cggcctcccc gcccgaaagc cgaggcgcgc tttcgcctcg ccgccgagcg
     2941 tgaagcactc gccgagagcc gcgacgatcc tcgcgatccc gacgaattcg gcgccgagcc
     3001 cgatctcgcc tacgccgcgc ccggcgttca gcatcgtgtg ttgcgacgcc tgcgccgcgg
     3061 ccagattccc gagcagggct tgctcgatct gcacggcaag acccaggccg ccgcgcacga
     3121 ggaactcgcg gaatttctca ccgccgccgg cgttcgcggc ctgcaatgcg tgcgcatcgt
     3181 tcacggcaag ggccaccgtt cgggtccggc aggaccggtg ctcaagcgtg ccgtcgcgcg
     3241 ctggctcgca cgtcgcaacg acgtgctcgc ctacaccagc gcgcgcccgg tggacggcgg
     3301 caccggcgcc gtctacgtgc tcctgcgccg ccgcgccgct cgcgcttccg ccatccagga
     3361 tgacgcccgc actcgctctt gatgattacg aaaacggggt gttactcagc ctgcgaacgc
     3421 cgccgccatt tgttcctgct ctgacttggg aatcttcagc gccgcggcct ctcgccgcca
     3481 tccggaaacc gcctgacgca gctgtttcca tattgccaac gcaactgcat ccgacaaacc
     3541 gtaatcccgg caggcccgca tggcaatgtc cacgtcacaa tgggtatccg tctcgttgat
     3601 ggcgagcgtc aactcgtccc gctccggcat gggattgata tcgtaggcag gcgaaagccg
     3661 gaagccttgc ggcacgagca gaaagccgtg attgcgcaag tggtcgtcgg tattgtggat
     3721 caagatgctg aaaacgacgc gccggaacaa ctcttcgcag tcggcgcggg tatcggcgcc
     3781 acgcgactgc agcaagtcca ccaattccag ataactcgcg ccggcttcac catcctcgcg
     3841 ctgggtcagc gtcatcgccg acacgaaggc tcgacggcgt ccccgcggcg tgcggtcgaa
     3901 acgttgcgcc agaaacgtcg tatacggact gctgccaagc cgcagcggac gaacgggcgg
     3961 cacgcgaata ccggccttcg cggccaaacg gtgcgcgagc agctcccagg cgcccatgtc
     4021 acgcgtgtca ctgcgactcg gaaacttggc gacgcataaa gcaccgttcc cgtcgcgcac
     4081 cgaggccttg ggccgcgccc cgccgaggga actgcccggg gcgaacaatt gtgtgagcca
     4141 tcgcgattcc tctctttcct tgccgccttc gagttcggct tcgaacttca gactggccgc
     4201 ttgcagttca cgcagcgaaa caacgggcgg ggccgccagc gcatcgctcc catcgagaaa
     4261 cggaccgtcc gccacgcgaa aacgcagcgc gcccatgcgc gtttcatcgg gaaccccgag
     4321 caggtaatcc catcccgtca aagtgcgggt ccggcgtttt tccgctcgtg cgcgtgcact
     4381 ctcccgccgc tgcatcagga cgcgccccca ccggtcgggc gaagagtcga gaaagacgcc
     4441 aaagccggcg cggccgcggg tcggatattg c
//
LOCUS       DASUJG010000267         2630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1425461, whole genome shotgun sequence.
ACCESSION   DASUJG010000267 DASUJG010000000
VERSION     DASUJG010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1425461"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="VFX38_05735"
     CDS             <1..564
                     /locus_tag="VFX38_05735"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEX5314388.1"
                     /translation="GWAGAVGPLRLLAFYGGYRSIATIISPLLVATGDARRNFQFTLF
                     AVCILPPLFYLGARWGATGVAAGWIVGFPVAMFPAYRRAFQIMEMRWSDYGAALRPAL
                     TATAVMALAVLGVRAANPASLGIGLSFAAEVIVGAAVYMTVVMLRYRDRVQSFLTLVK
                     SLRSRGDESERSVAADAGSPGVELASG"
     gene            complement(567..1832)
                     /locus_tag="VFX38_05740"
     CDS             complement(567..1832)
                     /locus_tag="VFX38_05740"
                     /inference="COORDINATES: protein motif:HMM:NF026968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX5314389.1"
                     /translation="MSQQRNQRNQRDRLERWLSQHAGGRPVAIVLGASPNGLSFLRSL
                     GRRGIPVLLIDADPLVASFSRYGTSISIARGPGESETLTGLLERIGRRLRTPGVLFST
                     ADHYCLMLARHEASLSRYYRFLQPDLATIEAILDKSTQYRRAEAAGIPIPPTFYPTTA
                     DDIEAIAGQVRYPAILKPYQPDHRPAAMREKSIKVIHAYSAQDLRAGFEQAAALGFQV
                     MVQETIPGDDSHFFGYWGFWDSEGRERAWVTKRKLRQSPPGFGDGSMQETIDAPEVAE
                     VSRKLLRAFNYRGFVGVEFKRDPRDGQLLLIEINPRTESGNQLAISAGVDLPWIGYRY
                     LTGQTPAEPEPLSPPFTPGVVFVNEEWDLKAFLAMRSTGTLTLAGWVQSLRGVRSHAL
                     GAWDDPMPALVIAGRIARAAARRTLRLPR"
     gene            complement(1845..>2630)
                     /locus_tag="VFX38_05745"
     CDS             complement(1845..>2630)
                     /locus_tag="VFX38_05745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5314390.1"
                     /translation="VLRKPHIITMHGGRYYAGRRRRRLALRWSARRSRALVGVSTTTA
                     AELADHLWLPRARVRVVHNGVRPETGEREPVRRELGIADDERLVVAIGNLYPVKAHAM
                     LLRALVQLDENATTQRWRVAIAGRGEEEASLRKLAAELGIADRVHLLGYRADVPNVLA
                     AGDVYVMPSLSEGLPLALIEAMFAGKPVVASNVGGIPEVVTDTVDGLLVAPADPDALA
                     TALAKVFADGQLADRLGAAARRRAEAGFGVERMVDEYEKLYGF"
BASE COUNT          415 a          894 c          865 g          456 t
ORIGIN      
        1 ggctgggcgg gtgccgttgg gccgctgcgg ctgctcgcgt tctacggcgg ctatcgatcg
       61 atcgcaacca tcatttcgcc gcttctcgtc gccaccggcg acgcgcgccg aaacttccag
      121 ttcacgctgt tcgcggtgtg catcctgccg ccgctgttct acctgggcgc acggtggggt
      181 gcgaccggtg ttgcggcggg ttggatcgtc ggatttcccg tcgcaatgtt cccggcgtac
      241 cgccgggcgt ttcagatcat ggagatgcgc tggtccgatt acggcgcggc gctgcgtccc
      301 gcgctcaccg ccacggccgt gatggcgctt gccgtgctcg gggtccgcgc ggcaaacccc
      361 gcctcgctcg gcatcggctt gtcattcgcc gccgaagtca tcgtcggcgc cgcggtgtac
      421 atgacagtag tcatgctccg ctaccgcgac agagttcaga gtttcctcac tctcgtgaaa
      481 tcgctccgct cgcggggcga cgaatcggag cgcagcgtgg cggccgacgc gggctcgccg
      541 ggcgtcgagc tggcctcagg ctgatctcag cgaggcagcc gcagggtgcg ccgcgccgca
      601 gcccgcgcaa tgcggcccgc gatcaccagc gccggcatcg gatcgtccca ggcgccgagc
      661 gcgtgcgacc gaactccgcg cagcgattgc acccagccgg ccagggtcag cgttcccgtc
      721 gagcgcatcg cgaggaatgc cttcaggtcc cactcttcgt tgacgaacac gacgcccggc
      781 gtgaacggcg gcgacaacgg ctccggctct gccggcgtct ggccggtcag atatcgatat
      841 ccaatccacg gcaggtcgac gccggcggag attgcgagct ggttgccgga ttccgtgcgc
      901 gggttgatct cgatgagcag caattgcccg tcccggggat cgcgtttgaa ttcgacgccg
      961 acgaatccgc gatagttgaa cgcgcgcaga agcttgcgac tcacctcggc gacctcgggc
     1021 gcgtcgatgg tctcctgcat cgatccgtcg ccgaacccgg gcgggctttg gcgcagcttg
     1081 cgcttggtca cccaggcacg ctcacgtcct tcggaatccc agaaccccca ataaccgaag
     1141 aagtgactgt cgtcgccggg gatggtctcc tgcaccatca cctgaaagcc gagcgctgct
     1201 gcctgctcga agccggcacg gagatcctgc gccgagtatg cgtggatcac cttgatcgat
     1261 ttctcccgca tcgctgccgg ccgatgatcc ggctggtacg gtttgaggat cgcgggatag
     1321 cgcacctggc cggcgatcgc ctcgatgtcg tccgccgtcg tcggatagaa ggtcggcgga
     1381 attggaatgc cggcagcctc ggcgcggcga tactgcgtgc tcttgtcgag gatcgcttcg
     1441 atcgttgcga gatcgggctg caggaatcga tagtatcgcg acagcgaggc ttcatgccgg
     1501 gcaagcatga gacaatagtg atcggccgtc gaaaagagca cgcccggcgt gcgcaatcgc
     1561 cgcccgattc gctcgagcaa tccggtcaga gtctcactct caccggggcc gcgcgcgatc
     1621 gagatgctcg tgccgtagcg cgagaagctc gcgaccagcg gatcggcatc gatcagcagg
     1681 accggaattc cacggcggcc cagacttctg agaaagctca acccgtttgg ggatgcgccc
     1741 agcacgatcg ccacgggacg cccgccggca tgctgggata gccagcgctc cagccgatcg
     1801 cgctgattgc gctgattgcg ctgctggctc atggggcgcg cacgtcagaa gccatacaac
     1861 ttctcgtatt catccaccat ccgctccacg ccgaaacccg cctcggcgcg gcggcgcgct
     1921 gccgcgccga ggcggtccgc gagctggccg tccgcgaaca ccttcgcgag tgcggtggcg
     1981 agtgcgtcgg gatcagcggg tgccaccagc aatccatcga cagtgtccgt cacgacctct
     2041 ggaatgccgc cgacgttgga cgccacaacc ggcttgcccg cgaacatcgc ctcgatcaac
     2101 gccagtggca gtccttcgga caaggaaggc atcacgtaca cgtcgcccgc agcgagcacg
     2161 ttgggtacat cggcgcgata gcccaggagg tgcacgcgat cggcgatgcc gagttccgcc
     2221 gcgagcttgc gcagcgatgc ctcttcttcg ccgcggccgg cgatcgccac gcgccagcgc
     2281 tgggtcgttg cgttctcgtc tagctgcacg agtgcacgca acaacatcgc atgcgccttg
     2341 accggataga ggttgccgat ggcgacgacg agtcgctcgt cgtcggcgat gccgagctcg
     2401 cggcgcacgg gttctcgctc gcccgtctcc ggtcgaacgc cgttgtgcac gacgcgaacg
     2461 cgcgctcgcg gcagccacag atggtcggcg agctccgcgg ccgtcgtcgt cgaaactccg
     2521 accagcgcac ggcttcgtct cgcgctccag cgcagcgcca ggcgccgccg gcgccggccg
     2581 gcgtagtatc gcccgccgtg catggtgatg atgtgcggct ttcgcagtac
//
LOCUS       DASUJG010000268         9116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1180851, whole genome shotgun sequence.
ACCESSION   DASUJG010000268 DASUJG010000000
VERSION     DASUJG010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9116
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180851"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..1148
                     /locus_tag="VFX38_05750"
     CDS             87..1148
                     /locus_tag="VFX38_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016267528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
                     /protein_id="HEX5314391.1"
                     /translation="MNYTISVLFRAIPAAMAALCVGYGLYCWTASAGSDYFVAGHVVT
                     FLGAICLALFATAATIIRQLVQWYNRFYRWTLPIFAYLVAAATIAFGLRMRVGPTDVF
                     VAGHVVFGLGLISACVATVATASTRFTLIPDNSRRAAGDPPPAAAFGAGTGRLLETIP
                     LILAAVGWAWAIYLLTRATDAAPFIAGHVLAGLAAICTSLIALVASVVRQIRNTYTRR
                     DARLWPALVVAMGTINIVWGIVIVALHREPYWFAPGFVLIGLGLICFSILSKVLLLAL
                     VWRRSFPLANRIPLIPVATALGCLFLAAFLFQAAVTNEAFFIPARVLVGLGAICFSLF
                     SIVSILESGTSSAGGGAPQ"
     gene            complement(1142..2134)
                     /locus_tag="VFX38_05755"
     CDS             complement(1142..2134)
                     /locus_tag="VFX38_05755"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX5314392.1"
                     /translation="MVAQIDKAYAKARPRKIVTRLASYALFEGRQVTTRGRWINPLIF
                     ALFAAECRLPALKRVRKPAFILGIGRSGTTVLGIALSVHREVGYLNEPKALWHFIHSH
                     EDVMGNFTSDESRYRLDAGDASPIAVRRARRLFGFYLAVTGSRRLVDKYPELIFRVPF
                     VRAIFPDARFILLLRNGSDNISSMDAWSNRNRETTAGEVHDWWGVNGRKWRLIRDELA
                     TTDPAFTGLQDVVAGLDRQLDKVAVEWILTMREALRQREKNSDRFFTVRYEDLAARPR
                     ATLAAIADFLELENDERFLAYGESLLRPSSGHPAPELHPRLRPLFEQTMRELGY"
     gene            2292..3377
                     /gene="hppD"
                     /locus_tag="VFX38_05760"
     CDS             2292..3377
                     /gene="hppD"
                     /locus_tag="VFX38_05760"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEX5314393.1"
                     /translation="MSAQPNRGMQVTTFENPMGVNGFEFVEFAAPDPKPLHALFGRLG
                     FAAVARHRSRKITLYRQGECNFLVNEEPESFAARFAAEHGPCACGFAIRFSKPATEVL
                     AAVLRNGAKEIGAEGAARAIEAPVIRGIGGCMLYLVDRHGAKGDVFEDYEWIQGAERN
                     PRGFGLTFIDHLTHNVRFGEMGKWADYYERLFNFREIKYFDIKGAKTGLVSKAMTAPD
                     GMVRIPLNESSDPKSQINEYLDEYHGEGIQHVALFTDTIYETVEAMGAAGVEFLDTPD
                     AYFEMVDERVPDHGEDVPRLAMNKILIDADMETKQKLLLQIFTQNCIGPIFFEIIQRK
                     GNEGFGEGNFTALFESIERDQMKRGVL"
     gene            3466..4770
                     /gene="hmgA"
                     /locus_tag="VFX38_05765"
     CDS             3466..4770
                     /gene="hmgA"
                     /locus_tag="VFX38_05765"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008437888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEX5314394.1"
                     /translation="MDAKPLKYMSGFGNEFATEALPGALPIGQNSPQRVPYGLRAEQF
                     SGTAFTAPKAMNRRSWLYRIHPAAVHGPFTAMAHAHFHNRFDEAPTPPERMRWDPFPA
                     PAEAADFLDGLYSVAGNGSAAEQTGCGLHVYNANRSMENRFFYNADGEMIFVPWEGRL
                     RLATELGAIEIAPLEIAVVPRGLRLRVELLDPVARGYVAENFGASLDLPERGPIGANS
                     LANARDFLAPVAAWEDVEDEFELVAKFQGRLWHAPLDHSPLDVVAWHGNNAPCKYPLA
                     RFNTIGSISYDHPDPSIFTVLTSQSDTPGTANMDFVIFPPRWLVAERTFRPPWFHRNV
                     MSEFMGLIEGKYDAKAGGFVPGGASLHNCMSGHGPDADSHAAALAEDTTKPRHVGETM
                     AFMFETRRVIHPTKQALESPQRHADYHGCWQGLGRNFDPTRP"
     gene            4849..5835
                     /locus_tag="VFX38_05770"
     CDS             4849..5835
                     /locus_tag="VFX38_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX5314395.1"
                     /translation="MKLGSLRNGERDGVLVVVKRDFSRAVEASEIAASLREALEDWNA
                     FASDLANLAAALETGEAGDAFDLDVAALGPPLPRAWEFLDGSAYLPHVERVRRARGAE
                     VPESFYTDPLMYQATSAGFLGPRDPIAVASEDYGIDLEAEVIVVTGEVPMGIAAENAG
                     ACIRLVGLVNDVSLRSLIPAELAKGFGFLQSKPRSALSPVFVTPDELGDAWHDEKLHL
                     PMRTRLNGQWLGEADCGEDMQFNFAQLIAHAAKTRPLAAGTLVGSGTIANQDTAKGAS
                     CLAERRTVEKLRDGEPSTPFLRFGDTVRIEVLDGEGRSIFGAIEQTVEQYRP"
     gene            5835..6476
                     /gene="maiA"
                     /locus_tag="VFX38_05775"
     CDS             5835..6476
                     /gene="maiA"
                     /locus_tag="VFX38_05775"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HEX5314396.1"
                     /translation="MELYGYWRSSAAYRVRIALHLKGLQATQHFVNLRADEQGSEVHR
                     RLDPLGRVPVLVDDGHRFTQSLAILEYLEERYPAPPLLPAAAADRTRVRALAQIVACD
                     MHPLNNLSTLRYLQETLGVSDEQKTAWYRYWIASGFTALEAMLAGDRRTGMFCHGDSP
                     GLADVCLVPQLYNARRFDCDLAAYPTIVRVDATCAKLDAFAQAVPEAQADAAL"
     gene            complement(6567..7001)
                     /locus_tag="VFX38_05780"
     CDS             complement(6567..7001)
                     /locus_tag="VFX38_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011994925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5314397.1"
                     /translation="MQAVEPSPRFHLAILVRDLAAARSFYGDLLGCAEGRSSESWVDF
                     DLGGHQLVCHQSAAGAPPPQRNPVDGDPVPVPHFGLVFGLAEWRALADRLEARGVAFA
                     LPPHIRFAGTAGEQGTFFLHDPSGNALEFKGFADLGRVFQKF"
     gene            complement(7009..7479)
                     /gene="smpB"
                     /locus_tag="VFX38_05785"
     CDS             complement(7009..7479)
                     /gene="smpB"
                     /locus_tag="VFX38_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX5314398.1"
                     /translation="MTKSAAPAPAIAENRRARHDYFIEERYEAGLALEGWEVKSLRAG
                     RANVSEAHALVRGGEVWLLGAHVNPLPSASTHVNPDPTRTRKLLLKAAEIRHLAGAVE
                     RRGYTLVPLRLYWKNGRAKLELGLAKGRKQHDKREAIKERDWARQQGRLLRDRH"
     gene            7566..8003
                     /locus_tag="VFX38_05790"
     CDS             7566..8003
                     /locus_tag="VFX38_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEX5314399.1"
                     /translation="MREIHRSALVSYPAEDMFALVNDVDSYPRFLTWCRSATAVATAD
                     NELQASLEFAKSGLHRRFTTRNRFEPGRAIRMDLVSGPFRVLSGNWEFRPLGAGGSKV
                     SLDLRFEFASRLLEAVFAPVFGEVMNSLVDAFVERANELHERP"
     gene            8000..8281
                     /locus_tag="VFX38_05795"
     CDS             8000..8281
                     /locus_tag="VFX38_05795"
                     /inference="COORDINATES: protein motif:HMM:NF015611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEX5314400.1"
                     /translation="MTDATIVIEVAPHPPELAGLAGIRIPAGSAIGDALAAIGIAAGS
                     AARIGIWGREASKSAVLADGDRIEFYRALIADPKEQRRARLRRAGKKRD"
     gene            complement(8278..8595)
                     /locus_tag="VFX38_05800"
     CDS             complement(8278..8595)
                     /locus_tag="VFX38_05800"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEX5314401.1"
                     /translation="MRKALLLAALLALAGCVYVPPVTQGNYLDAKDLSQLKVGMTTDQ
                     VTFLFGQPTLANPFYRDTWYYVYYVKAGAQSKAVIYRLTIQFQDGKVASFDTSAPISQ
                     SPS"
     gene            8670..>9116
                     /gene="fur"
                     /locus_tag="VFX38_05805"
     CDS             8670..>9116
                     /gene="fur"
                     /locus_tag="VFX38_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEX5314402.1"
                     /translation="MPEDKDLREAGLKVTAPRLKILRVLESSARRHMSAEDVYRALLD
                     AGEELNLATIYRVLTQFEAAGLVTRLRFEGGHSTFELDSGQHHDHILCVVCGRVDEFV
                     DDVIEHHQSAIAERLGYRMTDHSLHIYGICASCRDKGAASHPPDQAR"
BASE COUNT         1562 a         2909 c         2997 g         1648 t
ORIGIN      
        1 gccagcggct cggtcatcgc ttgcttgcgg cctcgatcct gatccggcat gatacgcctc
       61 ggtcctgcgc accaagagag agagggatga actacacgat cagcgtgctc ttccgggcga
      121 taccggccgc gatggcggcg ctctgcgtcg ggtacggcct ctactgctgg accgcgagcg
      181 cgggctcgga ttatttcgtc gccggtcatg tcgtcacctt tctcggcgcg atctgtctcg
      241 cgctgttcgc gaccgcggcg acgatcatcc gccagctcgt gcagtggtac aaccgttttt
      301 accgctggac gctgccgatt tttgcgtatc tcgttgcggc ggcgacgatt gccttcggct
      361 tgcggatgcg ggtggggcca accgatgttt tcgtcgcggg ccatgtggtc ttcggactcg
      421 ggctcatcag tgcctgcgtg gcgacggtcg cgaccgcctc gacccgcttc acgctgattc
      481 cggacaattc gcgccgcgcc gcgggcgatc cgccgcctgc cgcagcattc ggagccggca
      541 cgggcaggct tctcgaaacg attccgctca ttcttgcggc ggtcggctgg gcctgggcga
      601 tttatctcct cacgcgggcg accgacgcgg cgcctttcat cgccgggcac gtgctcgcgg
      661 gactcgccgc gatctgcacg agcctgatcg cgctcgtcgc gagcgtggtg cgccagatcc
      721 gcaataccta cacgcggcgc gacgcgcggc tgtggcccgc gctcgtcgtg gcgatgggaa
      781 cgatcaacat cgtctgggga atcgtgatcg ttgcgctgca tcgcgagccg tactggtttg
      841 cgcccggctt cgtgctgatc ggcctcggcc tcatctgctt cagcatcctg agcaaggtgc
      901 tgctgctcgc gctcgtgtgg cggcggagct tcccgctcgc gaaccgcatc ccgctgattc
      961 cggtcgcgac cgcgctcggc tgcctgttcc ttgccgcgtt cctgtttcag gcggccgtca
     1021 cgaatgaggc gtttttcatt cccgcgcggg tgctggtcgg gctcggcgcg atctgctttt
     1081 cgctcttttc gatcgtgagc atcctcgaaa gcggcacttc gtccgcgggc ggcggggcgc
     1141 ctcagtagcc gagttcgcgc atcgtctgct cgaagagcgg ccgcagccgc ggatgaagct
     1201 ccggagcggg gtgcccggag gacggccgca gcagcgattc tccatacgca agaaagcgtt
     1261 cgtcgttttc gagttcgaga aaatcggcaa tcgcggcgag cgttgcgcgc ggtcgggcgg
     1321 cgagatcttc gtagcgcacg gtgaaaaagc ggtcgctatt tttttcgcgc tggcgaaggg
     1381 cctcgcgcat cgtcaggatc cattccacgg cgaccttgtc gagttgtcgg tcgagccccg
     1441 cgacaacgtc ctgcagcccg gtgaatgcgg ggtccgttgt cgcgagttca tctcgtatca
     1501 ggcgccattt gcggccgttc acgccccacc aatcgtggac ttcgcccgcc gtcgtctcgc
     1561 ggttcctgtt ggaccatgcg tccatggaac tgatattgtc cgaaccgttg cgcaagagca
     1621 gaatgaagcg tgcatcggga aagatcgcgc gcacgaacgg cacgcgaaaa atgagttccg
     1681 ggtacttgtc cacgagtcgg cgtgagccgg tgaccgcgag atagaaacca aaaaggcgcc
     1741 gcgcgcgccg caccgcaatc ggggaggcgt cgcccgcgtc gagccggtag cgggactcgt
     1801 cgctggtgaa gttgcccatg acgtcttcgt gggaatgaat gaaatgccag agcgccttgg
     1861 gttcgttcag atagccgact tcgcggtgga cggacagggc gatgccgaga acggtggtgc
     1921 cgcttctgcc gatgcccagg atgaacgccg gctttcgcac ccgcttcagc gcgggcaagc
     1981 ggcattctgc cgcgaaaagc gcaaaaatca agggattgat ccagcgcccg cgagtcgtta
     2041 cctgccggcc ttcgaaaagc gcgtagctcg cgaggcgggt gacgatcttt ctcggccgtg
     2101 ccttggcgta ggctttgtcg atctgcgcaa ccatgtaccg gtccggggcg gaagtcgttc
     2161 ggagtcgtcg ggaaatcgta cccggcgccg caggcgcacc gcgaggggcg ctttcgatgg
     2221 cgcggccgga tgccttgcgg agcgcaggca gtataatcga aaccgaatct ggaatagcag
     2281 ggagttccgt catgagcgct cagcccaatc gcggcatgca ggtcactact ttcgaaaacc
     2341 ccatgggggt gaacggcttc gaattcgtcg aattcgcggc gccggacccg aagccgctcc
     2401 acgcgctttt cggacgcctc gggtttgccg ccgtggcccg ccatcgcagc cggaagatca
     2461 cgttgtatcg tcagggagaa tgcaattttc tggtgaacga ggaaccggag tctttcgccg
     2521 cccgcttcgc ggcggaacac ggcccctgcg cctgcggttt cgcaatccgc ttctcgaagc
     2581 ccgcgacgga agtgctcgcg gccgtgctcc gcaacggcgc caaggagatc ggcgccgagg
     2641 gagccgcccg cgccatcgaa gcgccggtaa tccgcggcat cggcggctgc atgctgtatc
     2701 tcgtggatcg ccatggcgcg aagggtgacg ttttcgaaga ctatgaatgg atccaagggg
     2761 ccgagcgcaa cccgcgcggc ttcggtctga ccttcatcga ccacctcacc cacaacgtgc
     2821 gcttcggcga aatgggcaag tgggccgact attacgagcg tctgttcaac ttccgcgaga
     2881 tcaagtactt cgacatcaag ggcgcgaaga ccgggctcgt gtccaaggcg atgaccgcgc
     2941 cggatggcat ggtgcgcatc ccgctcaacg aatcttccga tcccaagagc cagatcaacg
     3001 agtacctcga cgaatatcac ggcgagggaa ttcagcatgt ggcgctattc accgacacga
     3061 tctacgagac cgtcgaggcg atgggcgccg cgggcgtcga gttcctcgat acgcccgacg
     3121 cctacttcga gatggtggac gagcgcgtgc cggatcacgg cgaagatgtt ccgcggctcg
     3181 cgatgaacaa gatcctgatc gatgccgata tggaaacgaa gcagaaactc ctgctgcaga
     3241 tcttcacgca gaactgcatc gggccgatct tcttcgagat cattcagcgc aagggcaacg
     3301 aaggcttcgg cgaaggcaat ttcacggcgc tctttgaatc catcgagcgc gatcagatga
     3361 agcgcggcgt tctttgagga caaggcggat tcccgctggt ccgtggcatc gccgcgaagc
     3421 ggcgaagtcg aagggcagaa atatggtcat gacgtaggca aaatcatgga cgccaagcca
     3481 ttgaaataca tgagcgggtt cggcaacgag ttcgcgaccg aggcgctgcc cggagcgctt
     3541 cccatcggac agaactcacc gcagcgggtg ccctacgggc tgcgcgcgga gcagttttcg
     3601 ggcaccgcgt ttaccgcgcc gaaggcgatg aaccgccgct cctggctgta ccgtatccat
     3661 ccggccgccg tgcacggtcc cttcacggcg atggcgcatg cgcatttcca caatcgtttc
     3721 gacgaggctc cgacgccgcc cgagcgcatg cgctgggatc cgttcccggc cccggcggaa
     3781 gcggcggatt ttctcgacgg gctctacagc gttgccggca acggaagcgc cgccgagcag
     3841 acgggttgcg gtctgcatgt ctataacgcc aatcgctcga tggagaaccg gtttttctac
     3901 aacgcggacg gcgagatgat tttcgttccc tgggagggca ggctcaggct cgcgacggaa
     3961 ctcggggcca tcgagatcgc gccgctcgaa atcgccgtcg ttccccgcgg cctgcgcttg
     4021 cgggtggaat tgctcgatcc cgttgcccga ggctatgtcg cggaaaactt cggcgcgtcg
     4081 ctcgatctgc ccgaacgcgg gccgatcggc gccaactcgc tcgcgaacgc gcgcgacttt
     4141 ctcgctcccg tcgcggcctg ggaagatgtc gaggacgaat tcgaactcgt cgccaagttc
     4201 cagggacggc tctggcatgc gccgctcgat cactcgccgc tcgacgtggt cgcctggcac
     4261 ggcaacaacg caccctgcaa gtatcctctc gcgcgcttca acaccatcgg ctcgatcagc
     4321 tacgaccatc ccgatccgag catcttcacc gtgctcactt cgcaaagcga cactcccggg
     4381 acggccaaca tggacttcgt gatttttccg ccgcgctggc tggtcgcgga acgcacgttc
     4441 cgcccgccct ggttccatcg caacgtcatg agcgagttca tgggtctgat cgaagggaag
     4501 tacgacgcca aggccggcgg gttcgttccc ggcggcgcga gccttcacaa ctgcatgagc
     4561 gggcacgggc ccgacgccga cagccacgcc gcggccctgg cggaggacac gacgaagccg
     4621 cgacacgtcg gcgaaacgat ggccttcatg ttcgaaacgc ggcgcgtcat tcatccgacg
     4681 aagcaggcgc tggagtcgcc gcagcgccac gccgattacc acggttgctg gcaaggcctc
     4741 ggaaggaatt tcgatccgac tcgcccgtga cttggagagc cggcggaccg gaatgcggcc
     4801 gcgttcggcg ataatgaagg gaatcatcga gcgattccgg gggcggcaat gaagctggga
     4861 agtctgcgaa acggcgagcg cgacggtgtt ctggtggtcg tgaagcgcga tttctcgcgc
     4921 gccgtggagg cgagcgaaat cgccgcgagt ctgcgcgaag cgctcgaaga ttggaacgcc
     4981 ttcgcctccg atctggcgaa tctcgcggcg gcgctcgaaa cgggcgaggc cggcgacgcc
     5041 ttcgatctgg acgttgcggc gctcgggccg ccgctgccgc gggcctggga atttctcgat
     5101 ggctcggcct atcttccgca tgtcgagcgc gtgcgccgcg cccgcggtgc cgaagtgccg
     5161 gagagcttct acaccgatcc cctcatgtat caggcgacga gcgccggttt tctcggcccg
     5221 cgcgatccca ttgcggtcgc gagcgaggac tacgggatcg atctcgaagc cgaggtgatc
     5281 gtcgtgaccg gcgaagtgcc gatggggatc gcggccgaaa atgccggcgc ctgcattcgt
     5341 ctcgtcggcc tcgtgaacga cgtgagtctg cgcagtctca ttccggccga actcgcgaag
     5401 ggcttcggct ttctgcaatc caagccgcgc tcggcgctct cgcccgtgtt cgtcacgccc
     5461 gatgaactcg gcgatgcctg gcacgatgaa aagttgcatc tgccgatgcg cacgcggctc
     5521 aacggccaat ggctgggcga ggcggactgc ggcgaggaca tgcaattcaa ctttgcacaa
     5581 ctgatcgcgc acgccgcgaa gacgcgcccc ctcgcggcgg gaaccctcgt cggttcgggc
     5641 acgatcgcca atcaggacac ggcgaagggc gcgtcctgcc tcgccgaacg gcggaccgtg
     5701 gagaagctgc gtgacggcga gccctcgacg ccgtttctgc gtttcggaga cacggtgcgg
     5761 atcgaagtgc tcgacggcga aggccgcagc atcttcggtg cgatcgagca gaccgtggag
     5821 cagtaccggc cttgatcgaa ctctacggct actggcgatc ctctgccgcc tatcgggtcc
     5881 gcattgcgct ccatctcaag ggactgcaag cgacgcagca tttcgtcaat ctgcgcgccg
     5941 acgagcaggg cagtgaggtc catcggcggc tcgatccgct cggccgcgtt ccggtgctcg
     6001 ttgacgacgg tcacaggttt acgcaatccc tcgccatcct cgaatacctc gaggagcgct
     6061 atcccgcgcc gccgctgctt ccggcggctg ccgccgatcg gacgcgggtg cgggcgctcg
     6121 cgcagatcgt cgcctgcgac atgcatccgc tgaacaatct ttccactctc cgatacctgc
     6181 aagaaaccct gggtgtctcg gacgagcaga aaaccgcctg gtatcggtac tggattgctt
     6241 cgggcttcac ggcactcgag gcgatgctcg ccggcgatcg gcgaacgggc atgttctgcc
     6301 acggcgacag ccccgggctt gcggacgtct gcctcgtgcc gcagctctac aacgcgcggc
     6361 gcttcgactg cgatctcgcg gcgtatccga cgatcgttcg ggtggacgcc acctgcgcga
     6421 aactcgatgc cttcgcgcaa gcggtgcccg aggcgcaggc ggacgccgcg ctctgagata
     6481 tatgcgcggg agtgcctgtt ccggcgtgag gcgactgcgg gcctctgccg catccgccgg
     6541 gtttcgaaaa attcacggca gctctttcag aatttttgga agacacggcc gagatcggcg
     6601 aagcccttga attccagcgc gttgccggac ggatcgtgga gaaaaaacgt gccctgttcg
     6661 cccgcggttc cggcgaagcg gatgtgcggc ggcagtgcga atgcgacgcc gcgtgcttcg
     6721 aggcgatcgg cgagcgcccg ccattcggcg agtccgaaca cgagaccgaa atgcggcacc
     6781 ggcacggggt cgccgtccac gggattgcgc tgcggaggag gcgcgcccgc cgcgctttgg
     6841 tgacagacga gctggtgacc gccgagatcg aagtccaccc agctttcgct gctgcgtccc
     6901 tcggcgcagc cgagcagatc gccgtaaaag ctgcgggcgg cggccagatc ccgcacgagt
     6961 atggcgagat ggaaccgcgg gctgggttct actgcttgca tcgtttgctc aatgccggtc
     7021 gcgcagcagc cggccctgct ggcgagccca gtcgcgctcc ttgatcgcct cacgcttgtc
     7081 gtgttgcttg cgccccttgg cgagtccgag ttcgagtttg gcgcgaccgt tcttccaata
     7141 caggcgcagc ggaacgagcg tatagccgcg ccgctcgacg gctcccgcaa gatgccggat
     7201 ctccgccgcc ttgagcagca gtttgcgggt gcgcgtcgga tccggattga catgcgtcga
     7261 ggcgctggga agaggattca cgtgcgcgcc gagcagccac acctcgccgc cgcgcaccag
     7321 cgcatgcgcc tcggaaacgt tggcgcgccc ggcgcgcagc gacttcactt cccagccctc
     7381 gagcgcgagc ccggcctcgt agcgctcctc gatgaaatag tcgtggcggg cgcgacggtt
     7441 ctcggcgatg gcgggtgcgg gggcggcgga cttcgtcatc gcatcattgt aggcggcggc
     7501 gctgtgatag catcacagga ttccgccggc gggcggccgg cttctttgtt cattgcggaa
     7561 agcccttgcg agaaattcac cgctcggcac tggtatcgta ccccgccgag gacatgtttg
     7621 ctttggtcaa cgacgtcgat tcctatccgc gttttctgac ttggtgccgc agcgcgaccg
     7681 ccgtcgcgac cgcggacaac gaattgcagg caagcctcga gtttgcgaaa agcggtctgc
     7741 accgccggtt cacgacccgc aaccgcttcg agcccggccg cgcaatccgc atggacctcg
     7801 tgagtggccc cttccgcgtg ctcagcggca actgggagtt ccgcccgctc ggggcgggag
     7861 gctcgaaggt gagcctcgat ttgcgtttcg aattcgcctc gcgcctgctc gaagcggtct
     7921 tcgcgccggt gttcggcgag gtgatgaatt cgctggtgga cgccttcgtc gagcgcgcga
     7981 acgaactgca cgagcgtccg tgacggacgc gacgatcgtg atcgaagtgg ccccgcatcc
     8041 tccggagctt gccggccttg ccggaatccg gattcccgcc ggctccgcga tcggcgatgc
     8101 gctcgcggcg atcggcatcg ccgccggctc cgcggcgcga atcggcatct gggggcggga
     8161 agcttcgaag agtgcggtgc tcgccgacgg cgatcgcatc gagttctatc gggcgctcat
     8221 cgccgacccg aaggagcagc gtcgcgcccg cctgcgccgc gcggggaaga aacgtgatta
     8281 gctcggcgac tgactgatcg gagcggaggt gtcgaaactc gcgaccttgc cgtcctggaa
     8341 ctggatcgtg agccgataga tcaccgcctt tgattgcgcg cccgctttca cgtagtagac
     8401 gtaataccag gtgtcgcgat aaaagggatt ggcgagcgtc ggctggccga agagaaaggt
     8461 cacctgatcg gtcgtcatgc cgaccttgag ctgactcaaa tccttggcat ccagataatt
     8521 gccctgggtg acgggcggca cgtagacgca gcccgcgagg gcgaggaggg cggcgaggag
     8581 aagtgcctta cgcatggctt tcgcgaaagc ggacgcagta ggccataata cagggtatgg
     8641 aggctcgcgg ccagcggccg ggaccgcaaa tgcccgaaga caaggacctg cgcgaggcgg
     8701 ggctcaaggt gacggcgccg cgcctgaaga ttctgcgcgt gctcgaatcg agcgcgcggc
     8761 ggcacatgag cgcggaggac gtgtaccggg cgctgctcga tgccggcgaa gaactcaacc
     8821 tcgcgacgat ctaccgggtg cttacccagt tcgaagccgc gggcctcgtc acccggcttc
     8881 gcttcgaggg cggacactcg accttcgaac tcgactccgg ccagcatcat gaccacatcc
     8941 tctgcgtcgt ctgcgggcgc gtcgacgaat tcgtcgacga cgtaatcgaa catcatcaga
     9001 gcgccatcgc cgagcggctc ggctaccgca tgaccgatca ttcgctgcac atctacggga
     9061 tctgcgcgag ctgccgcgac aaaggcgccg cttcgcaccc gccggatcag gcaagg
//
LOCUS       DASUJG010000269         5713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_937688, whole genome shotgun sequence.
ACCESSION   DASUJG010000269 DASUJG010000000
VERSION     DASUJG010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5713
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937688"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VFX38_05810"
     CDS             complement(<1..375)
                     /locus_tag="VFX38_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015047360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX5314403.1"
                     /translation="MFRKILIANRGEIACRVMQTCRRLGVATVAVYSDADRRALHVEE
                     ADEAVHIGPAPAAESYLDMDRILAAAKATGAEAIHPGYGFLSENSEFARRCRKAGVVF
                     IGPSSESMDAMASTSAAKSLMEK"
     gene            complement(368..784)
                     /locus_tag="VFX38_05815"
     CDS             complement(368..784)
                     /locus_tag="VFX38_05815"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX5314404.1"
                     /translation="MFVLDASVALSWLLPDERNDGTQTLFQRLVREAALVPAIWLFET
                     GNALRSAVRSKRITAEQAWIVADDLLALPVELDQIGREERLKRALNVALQTGVSIYDA
                     AYLELSQRRQLPLATLDRRLREACAELNIPVLPDNV"
     gene            complement(803..1075)
                     /locus_tag="VFX38_05820"
     CDS             complement(803..1075)
                     /locus_tag="VFX38_05820"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX5314405.1"
                     /translation="MPEVGAYEAKTHLPRLLDKVQEGERFVITRHGKPVAELVPVARR
                     DEEEIRRTLDRIRAHRAELAKQGVRLKDILEPGETLRELAHRGHRY"
     gene            complement(1120..1638)
                     /locus_tag="VFX38_05825"
     CDS             complement(1120..1638)
                     /locus_tag="VFX38_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008148385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5314406.1"
                     /translation="MQSPILNVADLELQSAKPEQMPKGKNAADYDIRWGEIASRIGAR
                     RLGYNLTVVAPGKRNCPFHSHREEEEMFFVLQGAGELRYGDERYPLKGGDVIACPPGG
                     PETAHQIINTGDTELRYLALSTLAPVEICEYPDSGKFGVYAELAPDSDGKPQRFRHLA
                     RRQDARDYWEGE"
     gene            complement(1734..2525)
                     /locus_tag="VFX38_05830"
     CDS             complement(1734..2525)
                     /locus_tag="VFX38_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5314407.1"
                     /translation="MTDGLLLQTDERGVARLTLNRAEKHNAFDDTLIAELTAKLEALD
                     ADEGVRAVVLTGAGATFSAGADLEWMRGMAKASREENRADALRLAKMLSALNFLSKPT
                     LARVNGAAYGGGVGLVACCDIAIGAEGAKFAFSEVKLGLAPATIAPYVVAAIGLRQAR
                     RLFVSGEIFDACEAARIGLLHHCVAADALDETVERTLHFLLKGGPAAQQESKALALRM
                     AGMTREAAERVDADNAALIARLRVSDEGQEGIGAFLDKRKADWLK"
     gene            complement(2613..3488)
                     /locus_tag="VFX38_05835"
     CDS             complement(2613..3488)
                     /locus_tag="VFX38_05835"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX5314408.1"
                     /translation="MRRTSCAAALVVILTLVSIAPAFGASSPAGAKAPAPAALTRGEV
                     VTIVRQEIDRAFALRDWHQYSRYIDEYRGADAEALFSLSALDRATAIGDARSPVRVIV
                     YADYECPYCKLFESETSPKLHERFDSEALFLFRFFPLGIHGEIARSEAIAGACVGRTA
                     GAQAFRRFTARIFAATGSNGKGTRDGAEEIAHTVLHDSGVHLDRSKLDQLYDDCVNGK
                     TGAALIAPDAAFRGVTGTPTIFVVNTASKEAWRLAGAVPAWVFETLIGNVIAGRPGDS
                     DWAIDRQRVRPPDAD"
     gene            complement(3583..5199)
                     /locus_tag="VFX38_05840"
     CDS             complement(3583..5199)
                     /locus_tag="VFX38_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012509746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX5314409.1"
                     /translation="MPILKSAIDTNGKDFAANREHMTGLVADLRDQLAKSALGGGEKA
                     RKKHTGRGKLLARDRIEALLDPGSPFLELSPLAAHGMYDDAAPAAGVVAGIGRVQGRE
                     VMVVANDATVKGGTYFPITVKKHLRAQEVALENRLPCIYLVDSGGAFLPLQDEVFPDK
                     EHFGRIFYNQARLSRERIPQIAVVMGSCTAGGAYVPAMCDEAIIVRNQGTIFLGGPPL
                     VKAATGEVVDSETLGGGEVHSKVSGVTDHLADDDAHALAIARDIVAHFNYTRPEWLTK
                     QASVEPRYPSDELYGVVPTETRFPYDVHEVVARIVDGSEFQEFKALYGKTLVTGFAHL
                     YGYPVGIIANNGILFSESALKGTHFIEICEQRRIPIVFLQNITGFMVGAKYEAGGIAK
                     DGAKMVTAVACATVPKFTVLIGGSFGAGNYAMCGRAYGARFLWMWPNARISVMGGEQA
                     ASVLATVKRDGIEARAGKWSDKEEEEFKQPLREQYESQGHPYYATARLWDDGVIDPAD
                     TRRILGLALATCENAPPNPTDAARYGVFRM"
     gene            complement(5212..5634)
                     /locus_tag="VFX38_05845"
     CDS             complement(5212..5634)
                     /locus_tag="VFX38_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX5314410.1"
                     /translation="MIILDTNVVSEPMKRGGNPAVEAWLNRQLAETLYLTTVNLAELL
                     VGLEFLPDGKRKKGLKSALNELITRLFGARILAFDREAAETYATLVGRARVAGHALSV
                     ADAQIAAIATACGYAVATRDTVPFAAVGVSTINPWKTI"
BASE COUNT          944 a         1807 c         1875 g         1087 t
ORIGIN      
        1 cttctccatg agcgatttcg ccgcgcttgt cgaagccatc gcatccatgc tttcggacga
       61 aggtccaatg aacacgacgc cggccttccg gcagcggcgc gcgaattcgc tgttctcgga
      121 gaggaagccg tagcccggat ggatcgcctc ggccccggtc gcttttgcgg cggcgagaat
      181 gcgatccatg tcgaggtagg attccgcggc cggcgccggg ccgatgtgta ccgcctcgtc
      241 ggcctcttcc acgtgcagcg cccggcggtc ggcatcggaa tatacggcca ccgtcgccac
      301 gccaaggcgc cggcaggtct gcatcacccg gcaggcgatt tcgccgcgat tggcgatcag
      361 tattttccta aacattgtcg ggcaataccg gtatgttcaa ttcggcgcag gcttcgcgca
      421 aacggcgatc cagcgtcgca aggggcaact ggcggcgctg ggaaagctcg aggtaagcgg
      481 catcataaat gctgactccg gtttgcagag cgacgttcag cgctcgcttc agccgttctt
      541 cacggccgat ctggtccagt tcgaccggga gggccagcaa atcatccgcg acaatccatg
      601 cctgctccgc cgtgatccgc ttgctgcgaa cggcgctgcg caacgcgttg cccgtttcga
      661 acagccagat tgcgggcacg agcgccgctt ctcgaacgag acgctggaat aacgtctggg
      721 ttccgtcgtt tcgctcgtcc gggagcagcc aggacagcgc aacggaggca tcgagtacaa
      781 acacggctgc gcccgcgcgt cgctagtagc ggtggccgcg atgcgcgagt tcgcgcaagg
      841 tttcgccggg ttccagaatg tccttcaatc gcactccttg tttcgcgagt tccgcgcgat
      901 gggcacgtat gcgatcaagg gtgcggcgaa tctcttcttc gtcgcgccgg gcgacgggaa
      961 ccagttcggc gaccggcttg ccgtgccggg tgatgacgaa acgctcgcct tcctgcactt
     1021 tatcgagcag gcggggaaga tgcgttttgg cctcgtaggc gcctacttcg ggcatgcggg
     1081 gtttatgtat agaccggttc agaccagtct aacatgggcc tattcgcctt cccagtagtc
     1141 gcgagcgtct tggcggcggg caagatggcg gaaccgctgc ggtttgccgt cgctgtcggg
     1201 ggccagttcg gcgtacacgc cgaacttgcc ggagtcggga tactcgcaga tttcgacggg
     1261 cgcgagggtg ctcagcgcca gatagcgcag ctcggtatcg ccggtattga tgatctggtg
     1321 cgcggtttcc ggaccgccgg gtgggcaggc gatcacgtcg ccgcctttca gcggatagcg
     1381 ctcgtcgccg tagcgcagtt cgcccgcgcc ttggagaacg aagaacattt cctcctcttc
     1441 gcgatgactg tggaagggac aattgcgctt gcccggcgcg accaccgtga ggttgtaacc
     1501 gagcctgcgc gcgccgatgc ggctcgcgat ttcgccccag cgaatgtcgt aatccgccgc
     1561 gtttttgcct ttcggcatct gctccggctt cgcggattgc agttctagat cggcaacgtt
     1621 caggatggga ctttgcatga acacttctct cgtcgttccc gcgcaggcgg gaatccagtc
     1681 ggtttcgtag tccggtaggc cggcataagc cgcgaagcgc cgttgccggc atcttacttg
     1741 agccagtccg ccttgcgttt gtcgaggaag gcgccgatgc cttcctggcc ttcgtcggag
     1801 acgcgcaggc gcgcgatgag tgcggcattg tcggcatcga cgcgttccgc ggcttcgcgt
     1861 gtcattccgg ccatccgcag cgccagcgcc ttcgattcct gctgcgccgc gggcccgccc
     1921 ttcaagagaa aatgcagcgt acgctccacg gtttcatcca gcgcgtccgc cgcgacgcaa
     1981 tgatgcagga gcccgatgcg cgccgcttcg cacgcgtcga aaatctcccc gctcacaaac
     2041 agccgccgcg cctggcgcaa accgattgcg gcgacgacgt aaggggcgat ggtggccggc
     2101 gcaagcccga gcttcacctc ggaaaacgcg aacttcgcgc cctcggcgcc gatcgcgatg
     2161 tcacagcagg cgaccagacc gacgccgccc ccgtaggcgg cgccgttgac gcgggccagc
     2221 gtcggcttgg aaagaaaatt gagggcggac agcattttcg cgagacgcag tgcatcggcc
     2281 cggttttcct cccggctcgc cttcgccatt ccgcgcatcc actcgaggtc ggcgccggct
     2341 gagaaagtcg cccccgctcc ggtgagcacc acggcgcgca cgccctcgtc cgcgtcgagc
     2401 gcttcgagct tcgccgtcag ctcggcgatc agcgtgtcgt cgaaggcatt gtgcttttcc
     2461 gcgcgattca gcgtgagccg cgcgactccg cgctcgtcgg tttgcagcaa aaggccatcg
     2521 gtcatgactt gcgtctgcgc cgggagtcga gtccagtcta gccgatgcgt cgtgcgggcc
     2581 gtgtgcgagg cgaaggacgc cggccccgcc gatcaatcgg catcgggcgg tcgaacccgc
     2641 tgccggtcga tggcccagtc ggaatcgccc ggccgccccg cgatcacgtt gccgatgagc
     2701 gtctcgaaaa cccaggccgg caccgcgccg gcgagccgcc atgcctcttt gctcgccgtg
     2761 ttcacgacga aaatcgtggg cgtgccggta acgccccgaa acgccgcatc cggcgcgatg
     2821 agcgcggcgc ccgttttgcc gttcacgcag tcatcgtaaa gctgatcgag tttcgagcga
     2881 tcgagatgga cgccgctgtc gtgcagcacg gtgtgcgcga tttcttccgc gccgtcccgg
     2941 gtacccttgc cgttgctgcc ggtcgcggcg aagattcggg cggtgaagcg ccggaaggcc
     3001 tgggcgcctg cggtccggcc gacgcaggcg ccggcgatcg cctcgctgcg ggcgatctcg
     3061 ccgtggatgc cgagcggaaa gaagcggaag aggaagagcg cctcggaatc gaaacgttcg
     3121 tgcagctttg gactggtctc gctttcgaac agcttgcaat acgggcattc gtagtccgcg
     3181 tagacgatga cccgcacggg cgaccttgca tcgccgatgg cggtggcgcg atcgagcgcg
     3241 gaaagcgaaa acagggcttc ggcatccgcg ccgcggtatt cgtcgatgta acgcgagtat
     3301 tggtgccaat cgcgcaacgc gaaggcgcgg tcgatctcct ggcgcacgat cgtcaccact
     3361 tcgccgcgcg tcagcgccgc gggagcggga gccttcgccc ctgccgggga agaagcaccg
     3421 aaagccggcg cgatcgatac cagcgtcagg atgacgacaa gtgccgccgc gcacgacgtt
     3481 cgtctcacgg cctgccgtgc cgtcgccggc gcggcccttg ccggcgattt cgcaggcaac
     3541 gtcatgccgc gtcgaacgaa tgggctggca tcatttttcc tgctacatgc ggaagacgcc
     3601 gtagcgggcg gcgtcggtcg gattgggcgg ggcattttcg catgtcgcga gggcgaggcc
     3661 gagaatgcgg cgggtatcgg cgggatcgat cacgccgtcg tcccagaggc gggcggtcgc
     3721 gtaataggga tggccctgcg attcgtattg ctcgcgcagc ggctgcttga attcctcttc
     3781 ttccttgtcg ctccatttgc cggccctggc ttcgatgccg tcgcgcttga cggttgcgag
     3841 caccgaggcc gcctgttcgc cgcccatgac gctgatgcgc gcgttcggcc acatccacag
     3901 aaaacgcgcg ccgtaggcac ggccgcacat ggcgtagttg ccggcgccga acgagccgcc
     3961 gatcagcacc gtgaatttcg gcacggtggc gcaggcgacg gccgtaacca tcttcgcacc
     4021 gtccttggcg atgccgccgg cttcgtattt ggcgccgacc atgaagccgg tgatgttctg
     4081 caaaaacacg atcggaatgc ggcgctgttc gcagatttcg atgaagtgcg tgcccttgag
     4141 cgcgctttcc gaaaatagaa tgccgttgtt ggcgataatc cccacgggat aaccgtagag
     4201 atgtgcaaag ccggtgacga gcgtcttgcc gtagagcgcc ttgaattcct ggaattcgga
     4261 gccgtccacg attcgagcga cgacttcgtg cacgtcgtag gggaagcggg tttccgtcgg
     4321 caccacgccg tagagttcgt cgcttggata acgcggttcg acgctggcct gcttcgtcag
     4381 ccactcgggg cgcgtgtagt taaagtgggc gacgatgtcg cgggcgatgg caagtgcatg
     4441 cgcgtcgtcg tcggcgagat ggtcggtgac gcccgagacc ttgctgtgta cctcgccgcc
     4501 gccgagcgtt tccgagtcca cgacttcgcc ggtcgcggcc tttacgagcg gcgggccgcc
     4561 gagaaagatc gtcccctggt tgcgcacgat gatcgcctcg tcgcacatcg cgggcacgta
     4621 ggcgccgccc gcggtgcagg agcccatgac aaccgcaatc tgcgggatgc gctcgcgcga
     4681 cagacgtgcc tggttgtaga agatgcgccc gaaatgttcc ttgtccggga agacttcgtc
     4741 ctgcaagggc aggaacgcgc cgcccgaatc cacgagataa atgcagggaa ggcgattttc
     4801 gagcgcgact tcctgtgcgc gcaagtgctt tttgaccgtg atcggaaagt aggtcccgcc
     4861 tttcacggtg gcgtcgttcg cgaccaccat cacctcgcgt ccctgcacgc gcccgatgcc
     4921 ggcgacgact ccggcggcgg gagcggcatc gtcgtacatg ccgtgcgcgg cgagcggcga
     4981 gagttcgagg aagggcgaac ccggatcgag cagcgcctcg atgcgatcgc gcgctaggag
     5041 cttgccgcgg ccggtgtgct ttttgcgcgc cttttcgccg cctcccagtg cggatttcgc
     5101 aagttgatca cgcaagtcgg ccaccagccc cgtcatgtgt tcgcggttgg cggcgaagtc
     5161 cttgccgttg gtgtcgatcg cggatttcag tatcggcata tcagtgtccc gtcagatcgt
     5221 tttccaaggg ttgatggtag acacgccgac cgccgcaaac ggcaccgtgt cccgcgttgc
     5281 cacggcgtag ccgcaagcgg tagcgatagc ggcaatttgc gcatcggcga cagacagtgc
     5341 atggccggca acgcgggcgc gccccaccag cgttgcatag gtttcggccg cctcgcgatc
     5401 gaaggccagg atgcgcgcgc cgaaaagtcg cgtgatcaat tcgttcagtg cgcttttgag
     5461 tcccttcttt cgtttgccgt cggggagaaa ttcgaggccg acgagaagtt cggccagatt
     5521 caccgtggtc aggtacaggg tctcggccaa ctgtcgattc aaccacgcct cgaccgcggg
     5581 attgccgccg cgtttcattg gctccgacac gacgttcgtg tcgagaatga tcactcaaac
     5641 tttccggctt cggttggtgc cgggtcgcgc gggacgtcga gttccaactc gcgaaggtgc
     5701 ttgcggaatc ccg
//
LOCUS       DASUJG010000270         3410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_325884, whole genome shotgun sequence.
ACCESSION   DASUJG010000270 DASUJG010000000
VERSION     DASUJG010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325884"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="VFX38_05850"
     CDS             complement(<1..740)
                     /locus_tag="VFX38_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HEX5314411.1"
                     /translation="MKLKFLILTAGLGLLPLVALASSHREAPFISSMPKVDATDFYMF
                     MSYEQGRDNYVTLIANYNPLQDAWGGPNYFTLDPTAVYDIRIDNNGDAKPDIVYRFQF
                     QITYKDLTVPAGTANTAVPLINIGPVSAGDRSALNRVETYTVTQYDGDPLTADGQALT
                     NATDGSSTFTKPVDNIGNKSIADYASYAGHYIYNVSIPGCSQPGRVFVGQRKDPFVVD
                     LGEIFDLVNLNPAGPRDGTRNALHDKNVT"
     gene            complement(1058..1744)
                     /locus_tag="VFX38_05855"
     CDS             complement(1058..1744)
                     /locus_tag="VFX38_05855"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX5314412.1"
                     /translation="MNYDNPELRDLLAGKYVLAQLRGRARARFERLMFARPDFVRAVA
                     RWEERLAPLAFATPPVIPRARVWRRIAREIRPPRRRRSMRSPLAAGGFAAAGIAAAAF
                     FVMLGLYLARPAPAPVSEIAVISSTQGAAQWVISVRGGRMHMQTVGKIRPPPGKSYQL
                     WMLPAGGAKPVSLGLLPASGSASEALSRRLRDVLASAAGLAVSIEPQGGSPTGLPTGP
                     VVYTAKLLPV"
     gene            complement(1741..2313)
                     /locus_tag="VFX38_05860"
     CDS             complement(1741..2313)
                     /locus_tag="VFX38_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006915224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5314413.1"
                     /translation="MDEAERDRSNARLVGWMAACARGEVAALQELYSALSPQLFALLL
                     RILQRRDLAEEALQETFMSVWSKASEYQAIRGQVATWALAIARYRAFDILRRERYEVA
                     LEPETLLALCDAETAKEDGDESPLSLEERRRLRRCLEALPAAQRSSVVLAYFWGRTQE
                     EIAARLDAPLGTVKSWVRRSLASLKRCLER"
     gene            2567..>3410
                     /locus_tag="VFX38_05865"
     CDS             2567..>3410
                     /locus_tag="VFX38_05865"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX5314414.1"
                     /translation="MRNNPLEASLDGDHRYDDRWGDPSLAQQQRETAEMKANLAALKK
                     IDSGRLSPAEQLNYRLFEYQLQDGIAAAGFADDDQGLITQLWGPQLMAGSVKQLRFET
                     LADYRNWIQRLQSFGSYMDAYTERLKQAVAKGVVQPRVVMARVPAEIQGSISKDPTQS
                     GFYYPFQEMPETIPAAEQAKLRTAAKAAITNVVNPAYEKFLAFFQNDYLPQARVKIGV
                     SSLPDGKAYYRYLVRHYTTTDMTPEQIHQLGLQEVARINAEMEKTMRESGFKGTMPEF
                     LHFLK"
BASE COUNT          638 a         1066 c         1097 g          609 t
ORIGIN      
        1 gtcacgttct tgtcatgcag ggcattgcgg gtgccgtcgc gcggcccggc cggattcaag
       61 ttcaccagat cgaaaatctc gccgagatcc acgacgaatg gatccttgcg ctggccgacg
      121 aacacccgtc cgggctgcga gcagccgggt atgctcacgt tgtagatata gtggcccgca
      181 taacttgcat aatcggcaat ggacttgttg ccgatgttgt cgaccggctt ggtgaaggtg
      241 ctcgaaccat cggtggcatt cgtaagcgcc tgcccgtcgg ccgtgagcgg atcgccgtcg
      301 tattgagtga cggtatacgt ttcgacccgg ttgagtgcac tgcgatcgcc ggccgatacc
      361 ggcccgatgt tgatcagcgg gacggccgtg ttcgcggtcc ccgcggggac cgtaagatcc
      421 ttgtaggtga tctggaactg aaagcgatac acgatgtccg gcttcgcgtc gccgttgttg
      481 tcgatgcgga tgtcgtacac cgccgtcgga tcgagcgtga agtagttggg gccgccccag
      541 gcgtcctgca gcggattgta gttggcgatg agcgtgacgt aattgtcccg accctgctca
      601 taactcatga acatgtagaa gtcggtggca tccactttgg gcatcgagct gataaaaggg
      661 gcctcgcggt ggctcgatgc caaggccacc aggggcagca gccccaggcc cgcggtcaga
      721 atcaggaatt tgagcttcat ggtttgtacc tcgctgggaa cacgtaagcc gccatcgcac
      781 ggtaaaacag aaaatccgtg cgcttccgtg tcgttgcgca ccaaggttcc tcacggatcg
      841 gaccggcacg cctcgacacg gcagggctcc gtttcaggga accctgttcg gatatacgcg
      901 gatgcggcgg tttcggatgc gacggttgag ggcgcgagct gcgtttgatc gagttacggg
      961 gaaatcaaac cggcgtctgc cggaaacgat ccgattggtt tcagggtttt ccggcgcctt
     1021 cgctcctgcc gatgccggag cgaggcgcgc tccccgttca gacggggaga agtttggcgg
     1081 tgtagacgac gggaccggtc ggaagccccg tgggcgaacc gccttgcggt tcgatgctga
     1141 ccgcgagccc ggcggcgctc gcgagcacgt cgcgcagccg gcgggataac gcttcgcttg
     1201 ccgagccgga ggcgggcaga agaccgagcg acacgggttt tgcgcccccg gcgggcagca
     1261 tccagagttg atagctcttg cccggcggtg gcctgatctt tcctaccgtt tgcatgtgca
     1321 tgcgtccgcc gcgcaccgag atgacccatt gcgccgcccc ctgggtcgag gaaatcacgg
     1381 cgatctcgct cacgggagca ggcgcgggac gagcgagata gagcccgagc atgacgaaga
     1441 atgccgccgc ggcgattccg gccgcggcga atccgccggc ggcgagcggc gaacgcatcg
     1501 agcggcggcg acgcggcggg cgaatttcgc gggcgatccg ccgccaaacg cgcgcgcgcg
     1561 ggatgacggg tggcgtcgca aaagcgagcg gcgcgaggcg ctcctcccat ctggccaccg
     1621 cccgcacgaa atccgggcgt gcgaacatca gccgctcgaa acgggcgcgc gcgcggccgc
     1681 gcaactgcgc cagcacgtac ttcccggcca gaaggtcgcg cagttccggg ttgtcgtagt
     1741 tcatcgttcc agacaccgtt tcaggctcgc caaactacgg cgtacccagc tcttgaccgt
     1801 tccgagcgga gcatcgaggc gggcagcaat ttcttcctgc gtgcgacccc agaaatacgc
     1861 gagcacgaca ctcgagcgct gagcggcggg aagcgcttcg aggcagcggc gcagacggcg
     1921 gcgttcctcc aggctcagcg gagattcgtc gccgtcctct ttcgcggtct cggcatcgca
     1981 cagggcgagg agcgtttcgg gttccagcgc aacttcgtag cgctcgcggc gcagaatatc
     2041 aaaggcccgg taacgggcga tggcgagcgc ccacgtcgcc acctgacccc gaatcgcctg
     2101 atactccgaa gccttgctcc agacactcat gaacgtttcc tgcagggctt cctcggcgag
     2161 gtcccttcgc tggagaatac gcagcagcag agcgaatagt tgcggcgaca gtgccgagta
     2221 gagttcctgg agtgccgcga cttcgccgcg tgcgcaggcc gccatccatc ccaccagacg
     2281 ggcattgctg cggtcgcgct cggcctcatc cacggttggt attcgtcttg acgatcgctt
     2341 cgcaagggcc tcgttttacc atacttggcc cctcctgagc gaggccggcc cgatcgcggt
     2401 atagtctcaa gccgcctccc cgatgatcgt ccgagccatg cgaccgacat cctttgccct
     2461 gcttggcctg ttccttgccg gagcggccaa cgcagcctct gcaccgccgc cttcgtccgc
     2521 cccagcccgg ttgcgtgccc tgattgctca tgcctggcag caggagatgc gcaacaatcc
     2581 gctcgaggct tccctcgacg gcgatcaccg ctacgacgac cgctgggggg atccgagtct
     2641 cgcacagcag cagcgcgaaa ccgcggagat gaaggcgaac ctcgcggcgc tcaagaagat
     2701 cgattccggc cgattgagtc ccgccgaaca gttgaactat cgtctcttcg aataccagtt
     2761 gcaggacggg atcgcggccg caggcttcgc cgatgacgat caggggttga tcacccagct
     2821 ctgggggccg cagctcatgg cgggcagcgt gaagcaactg cgcttcgaga ccctcgccga
     2881 ctaccgaaac tggatccagc gcctgcaaag tttcggcagc tacatggacg cgtacacgga
     2941 gcgcctgaaa caggccgttg caaaaggcgt ggttcagccg cgggtggtga tggcgcgggt
     3001 gccggcggaa atccagggct ccatttcgaa agacccgacg caaagcggat tttattatcc
     3061 gttccaagag atgccggaga cgattccggc cgcggaacag gccaagctgc gcaccgcggc
     3121 aaaggccgcg atcacgaacg tcgtcaatcc ggcctacgag aagtttctcg cgtttttcca
     3181 gaacgattac ctgccgcagg cgcgtgtcaa gatcggcgtt tccagtctgc ccgacggcaa
     3241 ggcgtactac cgctatctgg tgcgccatta cacgacgacc gacatgacgc ccgagcagat
     3301 ccatcagctc ggcctgcagg aagtcgcgcg gatcaacgcc gagatggaaa aaacgatgcg
     3361 ggaaagcggg ttcaagggca cgatgcccga atttctccat ttcctgaaaa
//
LOCUS       DASUJG010000271         2251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_325995, whole genome shotgun sequence.
ACCESSION   DASUJG010000271 DASUJG010000000
VERSION     DASUJG010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325995"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..151)
                     /locus_tag="VFX38_05870"
     CDS             complement(<1..151)
                     /locus_tag="VFX38_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5314415.1"
                     /translation="MTDRRSGAVVLALEHISLAFGGVRALTDISFDVREHEVRAIIGP
                     NGAGKS"
     gene            complement(208..2148)
                     /locus_tag="VFX38_05875"
     CDS             complement(208..2148)
                     /locus_tag="VFX38_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5314416.1"
                     /translation="MDARADTFPGLLLKNARERGDRPAMREKSLGIWQTWSWSEIAAE
                     VRALACGLAAKGFRRGMHLAIIGDNRPRLYWSMAAAQALGGVPVPMYQDAPAAEFIFV
                     LNDAAIAYAIVEDQEQVDKMLEAKGEVPTLEHIYYDDPRGLRNYEGVTSFEALQAAGR
                     EFDHAHPDFFDTEIAHGSPDDVSVMLYTSGTTGKPKGVCQTHASFAAAAGGGATFDGL
                     VETDSVLSYLPMAWVGDHLFSYAQALIAGFTINCPESADTVMTDLREIGPTYYFAPPR
                     IFENLLTQLTIRMEDASALNRWLFRRFMAVARRCGGDILEGKPVPVTDRLLYALGNLF
                     IYAPLRNVLGMSRLRVAYTAGAAIGPDLFRFYRSIGINLKQLYGLTETCAYGCLQPNG
                     DVRLDTVGRAAPGVEIRIADSGEVLLRGPMLLKEYYKRPDATAESMDGEGYFHTGDAG
                     VFDAAGELRIIDRAKDVGKLAGGAMFAPNYIENKLKFFPHIREAVAFGNGRDRVCAFI
                     NIDFGSVGDWAERRGLAYSGYTDLAAKPEVHALVRECVEQVNAALAAEPGLSDTQVSR
                     FLILHKELDPDDDELTRTRKVRRRFIAEKYQVLIDALYSDRKVQHVETQVKFEDGRQG
                     IVAADLAIEDAKRFPPRVARAA"
BASE COUNT          355 a          737 c          761 g          398 t
ORIGIN      
        1 agctcttgcc cgcaccgttg gggccgatga tcgcgcgtac ttcgtgctcg cggacatcga
       61 agctgatgtc ggtgagcgcc ctgactccgc cgaaggcgag cgaaatgtgc tcgagtgcga
      121 gcacgacggc tccgctgcgg cggtcggtca tcggcttcgt ttcaggtcga tcatcgggtg
      181 cgctcggggc cgttcatcgc acgcgcgtca ggccgcccgc gcgacgcgcg gcgggaaccg
      241 cttcgcgtcc tcgatcgcga ggtcggcagc gacgatgccc tggcgcccat cctcgaattt
      301 gacctgcgtt tcgacgtgct ggaccttgcg gtccgagtag agcgcgtcga tcaggacctg
      361 gtacttctcg gcgatgaacc ggcgacggac cttgcgcgtg cgcgtgagct cgtcgtcgtc
      421 gggatcgagc tccttgtgca ggatcaggaa gcggctgacc tgcgtgtcgg aaaggccggg
      481 ctccgcggcg agcgccgcgt tcacctgctc gacgcattca cggacgagcg catgcacctc
      541 gggcttcgcg gcgaggtcgg tgtaaccgga ataggcaagg ccacgccgct cggcccaatc
      601 gccgaccgat ccgaaatcga tgttgatgaa cgcgcagacg cgatcacgcc cgttgccgaa
      661 cgcgaccgcc tcacggatgt gggggaagaa cttcagcttg ttctcgatgt agttgggcgc
      721 gaacatcgcc ccgccggcca gcttgccgac gtccttggcg cggtcgatga tccgaagctc
      781 gccggcggcg tcgaacacgc ctgcatcacc ggtgtggaag tagccctcgc cgtccatcga
      841 ctcggcggtt gcgtcggggc gcttgtagta ctccttcagc agcatcggcc cgcgcagcag
      901 gacctcgccg ctgtcggcga tcctgatctc gacccccggg gcggcgcgcc cgacggtatc
      961 aaggcgcaca tcgccgttgg gctgcaggca gccgtaggca caggtttcgg tcaacccgta
     1021 gagctgcttc aggttgattc cgatcgaacg gtagaagcgg aacaggtcgg ggccgatcgc
     1081 cgcgcctgcg gtgtaggcga cccgcagtcg cgacatcccg agcacgttgc gcagcggcgc
     1141 gtagatgaac aggttgccga gcgcatacag gagacggtcg gtgaccggga ccggcttgcc
     1201 ctccaggatg tcgccgccgc agcggcgcgc gaccgccatg aagcgcctga acagccagcg
     1261 attgagcgca ctcgcgtctt ccatccggat cgtgagttgc gtgagcagat tctcgaagat
     1321 ccgcggcggt gcgaagtaat aggtcggccc gatttccctc agatcggtca tcaccgtgtc
     1381 ggccgattcg ggacagttga tcgtgaagcc ggcgatcaac gcctgtgcgt acgaaaacag
     1441 gtgatccccg acccaggcca tcggcagata ggagagcacg ctgtcggtct cgacgaggcc
     1501 gtcgaaggtt gcgccgccac cggcggcggc ggcgaacgag gcgtgggtct gacacactcc
     1561 tttaggcttg ccggtggttc cggaggtgta gagcattacc gatacgtcgt cgggggagcc
     1621 gtgcgcgatt tcggtgtcga agaagtccgg atgcgcgtgg tcgaactcgc gtcccgcggc
     1681 ctgcaacgcc tcgaagctcg tcacgccctc gtagttgcgc aatccgcgcg gatcgtcgta
     1741 gtagatgtgt tcgagcgtcg ggacctcgcc tttcgcctcg agcatcttgt cgacctgctc
     1801 ctggtcctcg acgatcgcgt acgcgatcgc tgcgtcgttc aggacgaaga tgaactcggc
     1861 cgccggggcg tcctggtaca tcggcaccgg aacgcctccc agcgcctgcg cggcggccat
     1921 cgaccagtag aggcgcgggc ggttgtcacc gatgatcgcg aggtgcatcc cgcgccggaa
     1981 gcccttcgcg gcgagcccgc aggcgagtgc gcgaacctcg gcggcgatct cgctccagct
     2041 ccaggtctgc cagatgccaa gggatttctc gcgcatcgcg ggccggtcgc cgcgctcgcg
     2101 cgcattcttc agcaacagcc ccggaaaggt gtcggctcga gcgtccatct cctgctgtca
     2161 ccccccgttc cggctgcgtg gccgcgagcg cgtcggagcc gcccggcttg tgccggggat
     2221 tctaacggaa ctcgtttccc gcttgaccgc c
//
LOCUS       DASUJG010000272         2235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1671438, whole genome shotgun sequence.
ACCESSION   DASUJG010000272 DASUJG010000000
VERSION     DASUJG010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1671438"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VFX38_05880"
     CDS             <1..337
                     /locus_tag="VFX38_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020502815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S oxidoreductase"
                     /protein_id="HEX5314417.1"
                     /translation="LGKVVCHASCHQRVQNIGAKTREVLALVPGTTVELIQRCSGHDG
                     TYAVKRETYDNAMKIGRPVFRSIVAAAPDYYGSDCPLAGDQLEHGVGRDTGRGVTHPI
                     TMLRTAYAI"
     gene            421..1005
                     /locus_tag="VFX38_05885"
     CDS             421..1005
                     /locus_tag="VFX38_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HEX5314418.1"
                     /translation="MKKLEREDLMSLEEYAARREAFRREVMAHKEGRRLAIGPNVALY
                     FEDRMTMQYQVQEMLRAERIFEADGIREELDTYNPLIPDGTNLKATMMIEFEDEAERR
                     RELARLGGIEERVWIAAAGGSRSFGIPDEDLERRDSARTAAVHFLRFELDEETRRAIE
                     ARGQLAAGVDHPCYRETVDPLPAALLRSLAEDFA"
     gene            1102..>2235
                     /locus_tag="VFX38_05890"
     CDS             1102..>2235
                     /locus_tag="VFX38_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007190893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5314419.1"
                     /translation="MTARYEAADLEVLSGLDPVRRRPGMYTDTARPDHLALEVVDNSV
                     DEALAGFAKNITVTVQEDGSLEVEDDGRGMPVDRHPEEKIPGVELILTRLHAGAKFSQ
                     KQYRYAGGLHGVGVSVVNALSRNLEVWVRRGGIEYNMSFRGGEIASKLEEVGRVGKQN
                     TGTRLRFWPDPKYFDSPKFSLAKLRHALRAKAVLCSGLTVVLRNAATKEEERWCFEGG
                     LADYLAEATAGVETLPEAPFSGSFEGAREAAEWALAWCADGGPPLAESYANLIPTVQG
                     GTHVNGLRAGVTNAVREFSEFRNLVPRGVKLAPEDICDRLAYVLSVKMTDPQFSGQTK
                     ERLNSRECASFVGGVVKDALTLWLNQHIATGEQIASLAIESARE"
BASE COUNT          404 a          713 c          753 g          365 t
ORIGIN      
        1 gctgggcaag gtcgtctgcc acgcctcctg tcatcagcgg gtgcagaaca tcggcgccaa
       61 gacccgcgag gtgctcgcgc tcgttccggg aacgacggtc gagctgattc agcgctgctc
      121 cggacacgac ggtacctacg ccgtcaagcg cgaaacctac gacaatgcga tgaagatcgg
      181 ccggccggtt ttccgcagca tcgtcgcggc ggcgccggat tattacggca gcgactgccc
      241 gctcgccggc gatcagctcg aacacggcgt cgggcgcgac accggccgcg gcgtgaccca
      301 cccgatcacg atgctgcgca ccgcttacgc gatttaacgc aagtcacagc gattttttgc
      361 aactggattc gaaccttgtt tcccgctttt gcgccttggc cttgaatccc ggagagtgag
      421 ttgaagaaac tggaacgcga agatttgatg agtctggagg aatatgccgc gcggcgcgag
      481 gcgttccggc gtgaggtgat ggcgcacaag gaagggcggc gactcgcgat cggtcccaac
      541 gtcgccctct acttcgagga ccgcatgacg atgcagtatc aggtgcagga aatgctgcgc
      601 gccgaacgca tcttcgaggc ggacggcatc cgcgaggaac tggacaccta caatcccctg
      661 attccggacg gaaccaatct caaggcgacg atgatgatcg agttcgaaga cgaggcggag
      721 cgccggcgtg aactcgcgcg gctcggcgga atcgaggagc gggtgtggat tgcggcggcg
      781 ggcggctcgc ggagcttcgg gattcccgac gaagacctcg agcggcgcga cagcgcgcgc
      841 accgccgccg tacatttcct tcgtttcgaa ctggacgagg agacgcggcg cgcaatcgag
      901 gcgcgcggcc agcttgccgc cggtgtggat catccgtgct accgggaaac ggtggacccg
      961 ctgcccgccg ccctgctgcg gagtctggcc gaggatttcg cctaggcgcg ggcggcgggc
     1021 ccgggacctg ctgcgttcgc ggcgtacggc tgctgataaa atcagccggt ttatgcatgc
     1081 actgcgaacc ctcccccggt catgaccgcg cgctacgaag ctgccgatct cgaggtactt
     1141 tcggggctcg acccggtgcg ccggcgtccc ggcatgtaca ccgataccgc gcggcccgat
     1201 catctggcgc tcgaagtcgt ggacaacagc gtggacgagg cgctcgcggg cttcgcgaaa
     1261 aacatcaccg tgaccgtcca ggaagacggc tcgctcgagg tcgaggacga cgggcgcggc
     1321 atgccggtgg accgccatcc cgaggagaag atccctggcg tcgagctgat tctcacgcgc
     1381 ctgcacgcgg gggcgaaatt ctcgcagaag caataccgct acgccggcgg gctgcacggc
     1441 gtcggcgtgt ccgtggtcaa cgcgctcagc cgcaatctcg aggtctgggt gcggcgcgga
     1501 ggcatcgaat acaacatgtc gttccggggc ggcgagatcg cctcgaaact cgaagaggtc
     1561 ggccgcgtcg gcaaacagaa cacgggcacg cgcctgcgct tctggccgga cccgaagtat
     1621 ttcgactctc ccaaattcag tctcgccaaa cttcgccatg cgctccgcgc gaaggccgtc
     1681 ctctgctccg gtctcaccgt cgtgctgcgc aacgccgcga cgaaggagga ggaacgctgg
     1741 tgcttcgaag gcgggctcgc cgattatctc gccgaggcga cggccggggt cgaaacgctt
     1801 cccgaggcgc ctttttccgg aagtttcgag ggcgcgcgcg aggcggcgga atgggcgctc
     1861 gcgtggtgcg cggacggcgg accgccactc gccgaaagtt acgccaatct catcccgacg
     1921 gtacagggcg gcacgcacgt caacgggctg cgcgcgggcg tgacgaacgc ggtgcgcgaa
     1981 ttctcggagt ttcgcaatct cgttccgcgc ggcgtgaagc tcgcgcccga ggacatctgc
     2041 gatcgcctcg cctacgtatt gtcggtcaag atgaccgacc cgcagttttc cggccagacg
     2101 aaggaacgtc tcaattcgcg cgaatgcgcg agcttcgtcg gcggcgtggt caaggatgcg
     2161 ctcacgctgt ggctcaatca gcacatcgcg acgggcgagc agatcgcctc gctcgccatc
     2221 gaaagcgccc gcgag
//
LOCUS       DASUJG010000273         2003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_205085, whole genome shotgun sequence.
ACCESSION   DASUJG010000273 DASUJG010000000
VERSION     DASUJG010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205085"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /gene="miaB"
                     /locus_tag="VFX38_05895"
     CDS             <1..912
                     /gene="miaB"
                     /locus_tag="VFX38_05895"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX5314420.1"
                     /translation="PTAFVSVMEGCSKYCSFCVVPYTRGEEMSRPFDDALAEVWELAE
                     HGVREVTFLGQKVNSYRGRRHDGGLADLASLIHYTAAIEGIERIRFTTSHPVEFNERL
                     VRAFAEEEKLAAHLHLPVQSGSDRILAAMKRGYTALEYKSKLRRLRRARPGIAVSSDF
                     IVGFPGETEEDFEKTLRLVEECELDQSFSFLYSRRPGTPAAALAEVTPEAVKSERLHR
                     LQTLLDARGRGISEAMVGSTERVLVEKPSVKNPRELAGRTENNRWVNFSGPDASIGEF
                     VDVVITAALANSLRGRFVQAEPAARRA"
     gene            905..1879
                     /locus_tag="VFX38_05900"
     CDS             905..1879
                     /locus_tag="VFX38_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX5314421.1"
                     /translation="MPKPAPAEAEAGSEIVLEPADAERLAALCGSFDEHLRLIEEAFG
                     VTLANRGARFRIQGAAAATAQAMATLRKLYAATAAQSLDRAAVEAALAEARGEFEAGR
                     GEGGVRTRRGNVRPRGPRQTAYVAAMRTHDLTFGIGPAGTGKTFLAVACAVEALEADR
                     VRRIVLVRPAVEAGERLGFLPGDLAQKVDPYLRPIYDALYELLGFERVERLIERGVIE
                     IAPLAYMRGRTLNEAFIILDEAQNTTVEQMRMFLTRIGFESRAVVTGDVTQSDLPAHI
                     RPGLRHAVAVLKDVPAIAFTFFDARDVVRHPLVAEIVSAYERHESDGG"
BASE COUNT          334 a          683 c          663 g          323 t
ORIGIN      
        1 ccgacggcct tcgtttcggt catggagggg tgcagcaagt attgcagctt ttgcgtcgtg
       61 ccctacaccc ggggcgagga aatgagccgc cccttcgacg acgccctcgc cgaggtctgg
      121 gaactcgcgg aacacggcgt gcgcgaagtc acctttctcg gtcagaaagt gaactcctat
      181 cgcgggcgcc gccacgacgg cggcctcgcc gatctcgcga gcctgattca ctatacggcg
      241 gcgatcgagg gaatcgaacg aatccgcttc acgacctctc atccggtcga attcaacgaa
      301 cggctcgtgc gcgccttcgc ggaggaagaa aaactcgccg cccatctgca tctccccgtg
      361 caaagcggtt ccgaccggat tctcgcggcg atgaagcgcg gctacacggc gctcgaatac
      421 aaatcgaaac tgcggcgact gcgtcgcgcc cgcccgggga ttgccgtcag ctcggacttc
      481 atcgtcggct tccccggcga aaccgaagag gatttcgaaa agactctgcg tctcgtcgag
      541 gaatgcgaac tcgatcaatc gttcagcttc ctctacagcc gccgtcccgg aaccccggcc
      601 gcggccctcg ccgaggtgac gcccgaggcg gtcaagagcg agcgcctgca tcgcctgcag
      661 accctgctcg atgcgagagg gcgaggcatc agcgaggcga tggtggggag cacggagcgc
      721 gtgctcgtgg aaaagccgag cgtgaagaat ccgcgcgaac tcgcggggcg cacggaaaac
      781 aaccgctggg tgaatttctc gggaccggac gctagcatcg gcgagttcgt cgacgtcgtg
      841 atcacggcgg ctctcgcgaa ttcgctccgc ggccggttcg tgcaggcgga accggcggcc
      901 cgtcgtgcct aaacccgcgc ccgccgaagc cgaagccggg agcgaaatcg ttctcgaacc
      961 cgcggacgcc gagcggctcg cggcgctctg cggttccttc gacgagcacc tgcggctcat
     1021 cgaggaagcc ttcggcgtga cgctcgccaa tcgcggcgcg cgcttccgca tccagggagc
     1081 ggcggcagca acggcgcagg cgatggcgac gctgcgcaag ctctacgcgg cgacggcggc
     1141 gcagtcgctg gatcgcgccg cggtcgaagc cgcacttgcc gaggctcgcg gagaattcga
     1201 agcgggacga ggggagggcg gcgtacgcac gcgccgcggc aacgtccgtc cgcgcggccc
     1261 gcgccagact gcctacgtgg cggcgatgcg cacccacgat ctcaccttcg gcatcggtcc
     1321 cgccggcacc ggcaaaactt ttcttgccgt cgcctgtgcg gtcgaggcgc tcgaggccga
     1381 tcgcgtccgc cgcatcgtgc tggtgcgccc ggcggtggaa gcgggcgaac ggctcgggtt
     1441 cctgccgggc gatctcgcgc agaaggtcga tccgtatctg cgcccgatct acgatgcgct
     1501 ctacgaactc ctcggcttcg agcgcgtcga gcggctcatc gagcgcggcg tcatcgaaat
     1561 cgcgccgctt gcctacatgc gcgggcgcac cctcaacgag gctttcatca ttctcgacga
     1621 ggcgcagaac accacggtcg agcagatgcg catgtttctc acgcgcatcg gtttcgaatc
     1681 gcgggccgtc gtcaccggcg acgtgaccca gagcgacctt cccgcgcata ttcgccccgg
     1741 tttgcgccac gcggtcgcag tcctgaagga cgtgcccgcg atcgccttca ccttcttcga
     1801 cgcgcgcgac gtcgtccgcc atcccctcgt cgcagaaatc gtttccgcct acgaacggca
     1861 cgaatccgat ggcggctgaa cggatcggcg tgcagtatgc ctgtgcccgg cgcggcattc
     1921 cggccgcgcg ctcgctgcgg cgttgggcgt cgctggcact gggatcggcg cgcggtgtgg
     1981 tcacgatccg ggtggtcgac aac
//
LOCUS       DASUJG010000274         2063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1427143, whole genome shotgun sequence.
ACCESSION   DASUJG010000274 DASUJG010000000
VERSION     DASUJG010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1427143"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="VFX38_05905"
     CDS             <1..786
                     /locus_tag="VFX38_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005372213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5314422.1"
                     /translation="RYDKADLAHYFVKAGDGKMVPLSSVVTIAQTTVPQTLTQFNQLN
                     STTISGALRPGVSLGTALSYLQTQAKEVLPTGYFVNYEGESRTYVTEGNALLLTFFFA
                     LIVIYLILAALFESFRDPFIILVSVPMAICGALIFIYWGVASINIYTQVGLVTLIGLI
                     SKHGILIVRFANEIQRDEGLSIRESVEKAAAIRLRPILMTTAAMVVGVVPLLIASGAG
                     AAARFSIGLVVATGMGIGTLFTLFVVPMVYTFIAAKHKPLREE"
     gene            complement(892..1854)
                     /gene="epmA"
                     /locus_tag="VFX38_05910"
     CDS             complement(892..1854)
                     /gene="epmA"
                     /locus_tag="VFX38_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HEX5314423.1"
                     /translation="MSDSARDWLPVATRQTLAARAELMERLRVHFRERGILEVETPLL
                     SRAGSPELHIESLEVPRAFPDRAPGWLIPSPEFHLKRLLASGSGDVFSVARVFRAGEY
                     GRLHNVEFTLVEWYRLGAGLDELMDDIEALLAALLPAALLTHSPARITYREAFLRHAG
                     VDPFAASLGELRDAARDGDETDRDTLLDRIAVERVYPRLGDGRISLIHDFPASQASLA
                     RIRPGIPPVAERVEALVAGIELVNGFVELADCHEQRRRFDEARAVRTLAGRATPADDE
                     RFLAALEAGLPACAGAALGFDRLAMLAFGATSIADVLAFPADRA"
     gene            complement(1851..>2063)
                     /locus_tag="VFX38_05915"
     CDS             complement(1851..>2063)
                     /locus_tag="VFX38_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX5314424.1"
                     /translation="YNNRPLAVEAPNTVELVITQTDPGLRGDTSSGGNKPATLETGAV
                     VRVPLFVNEGEKVRVNTRTGEYLSRA"
BASE COUNT          374 a          666 c          678 g          345 t
ORIGIN      
        1 cgctacgaca aggcggatct cgcccattac ttcgtcaagg ccggcgacgg aaaaatggtg
       61 ccgctttcga gcgtggtgac gatcgcgcag acgacggtgc cacagacgct cacgcagttc
      121 aatcagctca actcgaccac gatcagcggc gccctgcgcc ccggggtttc gctcggcacc
      181 gcgctttcct acctgcagac acaggcgaag gaggttctgc cgacgggcta cttcgtgaac
      241 tacgaaggcg aatcgcgaac atatgtcacg gaaggcaacg ccctccttct gaccttcttc
      301 ttcgcgctga tcgtgattta cctgattctc gccgcgcttt tcgagagctt ccgcgatcct
      361 ttcatcattc tcgtgagcgt gccgatggca atctgcggcg cactcatctt catctactgg
      421 ggcgtggcga gcatcaatat ctacacccag gtgggattgg tgacgctgat cggactcatc
      481 agcaagcacg gaatcctgat cgtgcgattc gcgaacgaga tccagcgcga cgaaggactc
      541 tcgatccgcg agtcggtgga gaaggccgcc gcgatccgtc tgcgcccgat tctcatgacg
      601 actgcggcaa tggtggtcgg cgtggtgccg ctgctcatcg cgagcggcgc cggtgcggca
      661 gcgcgcttct cgatcggcct cgtggtcgcg accggcatgg gcatcggcac gctcttcacc
      721 ctcttcgtgg tgccgatggt ttacaccttc atcgcggcca agcacaagcc gctgcgcgag
      781 gaatagggcg cgcagactcg tcgccggcaa ttccccacac aagccgcagg cccgcataag
      841 cgcagcgttg cgggcaatcc ggaatcttcg gcgccaaatg ccggcaacgc gctaggcgcg
      901 atcggcgggg aaggcgagga cgtcggcgat gctggtggcg ccgaacgcga gcatcgcgag
      961 acgatcgaag ccgagcgcgg cgccggcaca ggccggaagc cctgcttcca gcgcggcgag
     1021 aaagcgttcg tcatcggccg gcgtagcgcg ccctgccaga gtacgcaccg cccgcgcctc
     1081 gtcgaaacgg cggcgctgtt cgtggcaatc ggcgagttcg acgaaaccgt tcacgagttc
     1141 gatgccggcg acgagcgcct cgacccgttc ggccacgggc ggaatgccgg ggcggatgcg
     1201 cgcaagcgag gcctgcgacg ccggaaagtc gtgaatgaga ctgatgcgcc catcgccgag
     1261 gcgtggatac acccgttcga cggcgatacg atcgagcagc gtgtcacggt cggtctcgtc
     1321 gccatcgcgc gcagcgtcgc gcagctcgcc gagcgaggcc gcaaatggat ccactccggc
     1381 atggcgaaga aaagcttcgc gataagtgat tcgcgccggc gaatgagtga gcagggcggc
     1441 cggaagcagc gccgcgagca gcgcctcgat gtcgtccatc agttcatcga gcccggcgcc
     1501 cagccggtac cattcgacga gcgtgaactc gacgttgtgc agccgcccgt attctccggc
     1561 gcgaaacacg cgggcgacgc tgaagacgtc gccgctgccg gaggcaagca ggcgtttcag
     1621 atggaattcc ggagaaggaa tcagccagcc gggagcgcga tcgggaaagg cccgcggcac
     1681 ctcgaggctc tcgatgtgaa gttccggcga gccggcacgc gacagcagcg gtgtttcgac
     1741 ttcgaggata ccgcgctcgc ggaaatgcac gcgcaagcgc tccatcagtt cggcacgggc
     1801 ggcgagcgtc tgccgcgtcg ccaccggcag ccagtcgcgc gccgaatcgc tcacgcgcgt
     1861 gagaggtact cgcccgttct ggtgttgacc ctgacctttt cgccttcgtt gacgaagagc
     1921 ggaacacgca cgacggcccc ggtttcgagc gtcgcgggct tgttcccgcc actcgaggta
     1981 tcgccgcgca gcccgggatc ggtctgcgtg atcacgagtt ccaccgtgtt cggcgcctcg
     2041 acggcgagcg ggcgattgtt gta
//
LOCUS       DASUJG010000275         2019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_82376, whole genome shotgun sequence.
ACCESSION   DASUJG010000275 DASUJG010000000
VERSION     DASUJG010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82376"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VFX38_05920"
     CDS             <1..498
                     /locus_tag="VFX38_05920"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5314425.1"
                     /translation="YAILPVILQPDRRRLLSYGWGASALALAGLACFLMWPTATPASD
                     IDWTRYPSVLFLKSVDQAGNACPSLHAAFAIFSALWLGRLLRDMGGRAPAQVLNACWC
                     VGILYSTIATRQHVAIDLAAGTALGAVAFALHRYFMLRTPRRAKRRSAQAPLPLPAPD
                     TRPGE"
     gene            complement(757..1203)
                     /gene="ssb"
                     /locus_tag="VFX38_05925"
     CDS             complement(757..1203)
                     /gene="ssb"
                     /locus_tag="VFX38_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX5314426.1"
                     /translation="MASRGVNKAILIGNLGADPEVRYTQGGMAVANLRIATSERWKNK
                     QSGEQQEHTEWHRVALFGKLGEIAGEYLKKGSQVYIEGRIRTRKWQGQDGQDKYTTEI
                     IADNMQMLGGRGEGGSYGARGENQSAEVAEERDIPGAPLEDDDIPF"
     gene            complement(1234..>2019)
                     /locus_tag="VFX38_05930"
     CDS             complement(1234..>2019)
                     /locus_tag="VFX38_05930"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5314427.1"
                     /translation="RTLADGRLLRLYGSIFALHSMLAALFLVIPLFLLRDSALGGANE
                     WMFYLPVLALGLAFMLPLVIYAEGRGRVKPVALGAVVALGAAALALGFLPASIFALGI
                     VLIVFFAAFTLMEAVLPALVSRLARPDARGTALGFYSTSQFLGIFVGGALGGWLQGHF
                     GPTGLCIFVAALALGWLPLVATLHLPPALASRMLAISVPDAAAAHDLARRFEAVAGVE
                     EALVFADDGVAYLRVDRERLDEAALAGVPTGTDAAPIARDPSR"
BASE COUNT          332 a          698 c          655 g          334 t
ORIGIN      
        1 tacgccatcc tgccggtgat cctgcaaccc gaccggcgcc ggcttctttc ctatggttgg
       61 ggtgcgagcg cgctcgccct tgccggcctc gcctgttttc tcatgtggcc gaccgcgacg
      121 cccgcctccg acatcgactg gacgcgctac ccctccgtgt tgttcctcaa gtccgtggat
      181 caggcgggca acgcctgtcc gtcgctccac gccgccttcg cgattttctc cgcgctctgg
      241 ttgggccgcc tgcttcggga catggggggg cgagcgccgg cgcaggtgtt gaacgcctgc
      301 tggtgcgtcg gcattctcta ctcgacgatt gccacccgcc agcacgtggc aatcgacctc
      361 gcggccggaa ccgcgctcgg cgcggtcgcc ttcgctctgc atcgctactt catgctccgc
      421 accccgcggc gcgcgaagcg gcgcagcgcg caagcgccgc tgccgctgcc ggcacccgat
      481 acccgtcccg gcgaatgagg gccggtttgc gacgagttgc tcgcgcgtcc gggagcggct
      541 ctcaggggtg tggcaggcaa agagtacacg gaccgccggt cgcacccgcc ttcacgatcc
      601 gggtatagct tctggtcgga ctctgacgat gcaattcgtt gcccggcacg ggatacgtct
      661 ttcgccactg cgccgtttgc aagcgcacat cgagacgaaa gcctgccggt tccgtaagca
      721 tggcattttc ctcaccgacc ggcgcgcggc agggcctcag aacggaatgt cgtcgtcttc
      781 gagcggagcg cccggaatgt cgcgctcctc cgcaacctcg gccgactgat tctcgccgcg
      841 cgcgccatag gaaccgccct cgccgcgtcc gccgagcatc tgcatgttgt ccgcaatgat
      901 ctcggtcgtg tacttgtcct gcccgtcctg cccctgccac ttgcgggtgc ggatgcgccc
      961 ttcaatgtac acctgcgagc ccttcttgag gtattcgccg gcgatctcgc cgagctttcc
     1021 gaagagcgcg acgcggtgcc attcggtgtg ttcctgctgc tcgcccgact gcttgttctt
     1081 ccagcgctcg ctcgttgcga tgcgaagatt ggcgaccgcc atgccgccct gggtgtagcg
     1141 tacctccgga tcggcaccga gattgccgat cagaattgcc ttgttgactc ctctcgaggc
     1201 catgtccggc tcctcccgtt cgaaggcttc attctacctg ctggggtcgc gggcgatcgg
     1261 cgcggcgtcc gtgccggtcg gcacgccggc gagcgccgcc tcgtcgagac gctcgcgatc
     1321 gactcgcaaa taggcaacgc cgtcgtcggc gaaaacgagc gcttcttcca ctccggcgac
     1381 ggcctcgaag cggcgcgcaa gatcgtgcgc ggcagcagcg tcgggcaccg agatcgcgag
     1441 cattcggctc gcgagcgccg gcggcaggtg aagcgtcgcg acgagcggca accacccaag
     1501 cgcgagcgcg gcgacgaaaa tgcagagccc cgtcggcccg aagtgcccct gcagccagcc
     1561 cccgagcgcg ccgccgacga aaatgccgag aaactgtgac gtcgaataaa agccgagcgc
     1621 cgttcctctg gcatcgggac gcgcgagccg cgagacgagc gcagggagga cggcctccat
     1681 cagggtgaag gcggcgaaga aaacgatgag cacgatgccg agagcaaaga tggatgcggg
     1741 cagaaatcca agcgcgagcg ccgccgcgcc gagtgcgacg acggcgccga gcgcgaccgg
     1801 tttcacccgt ccgcggcctt ccgcatagat cacgagcggg agcatgaagg cgagaccgag
     1861 cgcgagcacc ggcagataaa acatccactc gttcgcgccg ccgagcgccg agtcgcgaag
     1921 cagaaacagc ggaatcacca ggaagagcgc ggcgagcatg ctgtggagcg caaagatgga
     1981 gccgtagagc cgcagcagcc gtccatcggc gagcgtgcg
//
LOCUS       DASUJG010000276         3381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1305016, whole genome shotgun sequence.
ACCESSION   DASUJG010000276 DASUJG010000000
VERSION     DASUJG010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305016"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..780)
                     /gene="sufC"
                     /locus_tag="VFX38_05935"
     CDS             complement(34..780)
                     /gene="sufC"
                     /locus_tag="VFX38_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009849723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEX5314428.1"
                     /translation="MLAIENLRAGVGDTGILRGVDLEVPAGSVHAIMGPNGSGKSTLA
                     HVMAGRPGYEVTGGSVKLGERELLGLAPEERAREGLFLALQYPVEIPGVNNVYLLKAG
                     LNALRKHRGEAELDAYDFFKLARAKLAALGLDDSFLNRGVNEGFSGGEKKRNEVLQML
                     LFEPRFAVLDETDSGLDIDALRIVAAGVNGMRSPERGILLITQYQRLLDYIRPDAVHV
                     MIAGRIVKSGDAALARTLEERGYDWLREAA"
     gene            complement(792..2243)
                     /gene="sufB"
                     /locus_tag="VFX38_05940"
     CDS             complement(792..2243)
                     /gene="sufB"
                     /locus_tag="VFX38_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX5314429.1"
                     /translation="MATPEPQVSALLARRYKDGFYTDIESDTVAPGLDEDVIRLISAK
                     KGEPEWMCDWRLEAYRRWQEKSVPHWAHVHYPPIDYQAISYYSAPKSKDGKAPKSLDE
                     VDPKLLETYEKLGIPLHERAALAGVAVDAVFDSVSVATTFKEKLAKAGVIFCPISEAI
                     REHGELVRQYLGSVVPPGDNFYASLNSAVFTDGSFVFVPRGVRCPMELSTYFRINASN
                     TGQFERTLIIAEEGAYVSYLEGCTAPQRDENQLHAAVVELIALDGAEIKYSTVQNWYP
                     GDEEGRGGIYNFVTKRGDCRGARSKICWTQIETGAAITWKYPSVILRGDGSTGEFYSV
                     AVTNNCQQADTGTKMIHLGRDTTSTIISKGISAGRAQNAYRGLVRVAARAENARNYTQ
                     CDSLLMSDRCGAHTFPYIEVKNPTARVEHEATTSKIGEDQLFYLRSRGLSEEDAVNLV
                     VNGFCKSVFQKLPMEFAVEAGRLLAVSLEGAVG"
     gene            complement(2230..2697)
                     /locus_tag="VFX38_05945"
     CDS             complement(2230..2697)
                     /locus_tag="VFX38_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HEX5314430.1"
                     /translation="MSKLADYAALILATMAERPGRTYSASGLAAETGISLPTASKLLK
                     TLAHGGLVVSRRGAHGGYSLGRAPAAISAVDMIEAVEGPLAMTECTLSAGLCELEPSC
                     QLGSQWQSLSAAIRRALAEVTLADLVGPPLAPRLSLRRPLRIPIHAEKAHGHA"
     gene            complement(3045..>3381)
                     /locus_tag="VFX38_05950"
     CDS             complement(3045..>3381)
                     /locus_tag="VFX38_05950"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX5314431.1"
                     /translation="FTLLVHELATNATKYGALSRAGGRIAVEWTIEEPAAAPSLRFRW
                     FERGGPAVMKPNRRGFGRFLIEKAAAREFGAEPRVSFDPEGLAYEIKMPLSAVATTAP
                     TGNEAAKSA"
BASE COUNT          596 a         1047 c         1105 g          633 t
ORIGIN      
        1 cgggcagggc ggcaagaaag ggaacgtcga tgctcacgct gcttcccgca gccagtcgta
       61 tccgcgctcc tcgagggttc tcgcgagagc ggcgtcgccg gacttcacga tgcgcccggc
      121 gatcatgacg tgcaccgcgt cggggcggat gtaatcgagc aggcgctggt actgggtgat
      181 gagaagaatg ccgcgctcgg gggagcgcat tccgttcacg cccgcggcga cgattcgcag
      241 cgcatcgatg tccagccccg aatcggtttc gtcgagaacg gcgaagcgcg gttcgaagag
      301 cagcatctgc agaacttcgt tgcgtttctt ttcgccgccg gagaagcctt cgttcacgcc
      361 ccggttgaga aaggaatcgt cgagtccgag tgcggcgagt tttgcgcggg cgagcttgaa
      421 aaaatcgtag gcgtcgagtt cggcctcgcc gcgatgtttg cgcagcgcat tcaatcccgc
      481 cttcagaaga tacacgttgt tcacgcccgg aatctcgacc gggtattgca acgcaagaaa
      541 gagtccctcg cgggcccgct cttccggagc gagaccgagg agttcgcgct cgccgagctt
      601 cacgcttccg ccggtgacct cgtagcccgg acggcccgcc atcacgtgcg cgagagtgga
      661 cttgccggag ccgttcggcc ccatgatcgc gtgcacgctg cccgccggaa cttcgagatc
      721 gacgccgcgg agaatcccgg tatcgccgac cccggcgcgc aggttttcga ttgcgagcat
      781 gttcgtctcc atcagccgac cgccccctcg aggctcaccg cgagcaggcg cccggcctcg
      841 acggcgaatt ccatcgggag tttctggaac accgatttgc agaaaccgtt gacgacgaga
      901 ttcacggcgt cttcctcgga cagcccgcgg ctgcggagat agaacaactg atcctcgccg
      961 atcttcgacg tcgttgcttc gtgctcgacc cgcgcagtcg gattctttac ctcgatgtac
     1021 ggaaaggtgt gcgcgccgca gcgatcgctc atgagaaggg aatcgcattg cgtatagttg
     1081 cgcgcgtttt ccgccctggc ggcgacgcgc acgagcccgc gataggcgtt ctgtgcccgg
     1141 ccggcggaaa ttcccttgga aatgatcgtg ctcgtggtgt cgcgcccgag atggatcatc
     1201 ttggtgccgg tgtcggcctg ctggcagttg ttggtgaccg caaccgagta gaactcgccc
     1261 gtggaaccgt cgccgcggag aattacgctc gggtacttcc aggtgatggc cgcaccggtc
     1321 tcgatctgcg tccagcagat cttcgagcgg gcgccgcggc agtcgccgcg cttggtgacg
     1381 aaattgtaga tgccgccgcg gccctcctcg tcgccgggat accagttctg caccgtcgaa
     1441 tatttgatct cggcgccgtc gagcgcgatg agttcgacga cggccgcgtg caactgattt
     1501 tcgtcacgtt gtggggcagt gcaaccttca agataggaga cgtaagcgcc ttcctcggcg
     1561 atgatcaggg tgcgctcgaa ctggccggtg ttcgaggcgt tgatgcgaaa gtaggtggaa
     1621 agctccatcg ggcagcgcac gcccctcggc acgaatacga aggagccgtc ggtgaagacg
     1681 gccgagttga ggctcgcgta gaagttgtcg ccgggaggaa cgacggagcc gagatattgg
     1741 cgcaccagtt cgccatgttc gcgaatggcc tccgatatcg gacagaagat cacccccgcc
     1801 ttcgcgagtt tttccttgaa ggtcgtcgcc accgaaacgc tgtcgaagac cgcgtccacg
     1861 gcgacgccgg cgagcgcggc gcgttcgtgc agcggaatgc cgagcttctc gtaggtttcg
     1921 agaagtttgg ggtccacctc gtcgaggctc ttcggcgcct tgccgtcctt cgatttcggc
     1981 gccgagtaat aggaaatggc ctgatagtcg atcggcggat agtgcacatg cgcccagtgg
     2041 gggaccgact tttcctgcca gcgccggtag gcttcgagcc gccagtcgca catccattcg
     2101 ggctcgccct tcttggccga aatcaggcgg atgacatctt cgtcgagccc cggcgcgacc
     2161 gtgtcggact cgatgtcggt gtaaaaaccg tccttgtagc ggcgggcgag aagtgcgctt
     2221 acctgcggct caggcgtggc catgcgcctt ctccgcgtgg atcgggattc tcaacgggcg
     2281 ccgcagcgag agccgtggcg cgagcggcgg accgacgagg tcggcgagcg tgacttcggc
     2341 gagtgcgcgg cgaatcgcgg ccgacagact ctgccattgc gagccgagtt gacaggaggg
     2401 ttcgagttcg cagagtccgg cagagagcgt gcattcggtc atcgccagcg gtccctccac
     2461 cgcctcgatc atgtccacgg cgctgattgc ggcaggcgcc cgcccgaggc tgtagccgcc
     2521 gtgggcgccc cggcgcgaga cgacgagccc gccatgggcg agggttttca agagcttgct
     2581 cgcggtgggg aggctgatgc cggtttcggc cgcgagcccg gaggcggagt aggtgcgccc
     2641 gggccgctcg gccatggtgg cgagaatcag ggccgcataa tcggcgagct tgctcatccg
     2701 cagcatcggg atttcccgga ggagatcaat acaggaccat tttagttcta tacgacgccc
     2761 gaagcaagaa cttcgcccga tgcgacgccg gacgccaacg cctcgctgga ggcacggaaa
     2821 ggtccgaccg tttgattcaa tcccaagaat ctttgaaaaa tcctcgcgca agctggaaat
     2881 tcgccgggtt accggctgaa cgatccacga aagttcagcg gcttggagcg ccttatcaat
     2941 tccttctcat cgtttccgcg cagaggcggt ccggccaacc gagagactgg attcccgctt
     3001 tcgcgggaat gacgaaggaa tcacctcagg cgccacctgc ccggctaagc ggatttggcc
     3061 gcttcgtttc ctgtcggcgc cgtcgtcgcc accgccgaga gaggcatttt tatttcgtag
     3121 gccaaccctt cgggatcgaa gctgacacga ggttccgcac cgaattcccg cgccgcggcc
     3181 ttctcgatca ggaaacgccc gaagccccgg cggttcggct tcatgacggc gggcccgccg
     3241 cgctcgaacc aacggaagcg caggctcggg gccgccgctg gctcctcgat cgtccattcg
     3301 acggcaattc tgccgccggc ccgagacagg gcaccgtatt tggtggcgtt ggtggccaac
     3361 tcgtgcacga gcagcgtgaa g
//
LOCUS       DASUJG010000277         4959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_940090, whole genome shotgun sequence.
ACCESSION   DASUJG010000277 DASUJG010000000
VERSION     DASUJG010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940090"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VFX38_05955"
     CDS             <1..221
                     /locus_tag="VFX38_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="chaperone Hsp40; co-chaperone with DnaK;
                     Participates actively in the response to hyperosmotic and
                     heat shock by preventing the aggregation of
                     stress-denatured proteins and by disaggregating proteins,
                     also in an autonomous, dnaK-independent fashion; Derived
                     by automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX5314432.1"
                     /translation="RVRGKGLPLPGGGRGDLFCAVQVQTPVNLNEEEKELIRNLDEQL
                     RIGGSRHNPAEQESWTAKVKNFFEQLAG"
     gene            218..1039
                     /gene="dapB"
                     /locus_tag="VFX38_05960"
     CDS             218..1039
                     /gene="dapB"
                     /locus_tag="VFX38_05960"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX5314433.1"
                     /translation="MNAPSVKSGEPIRIALLGATGRMGREIVAAIRQAPDLRLVAALA
                     RADDESLGRDAGEIAGGAALGVALSGDLNAASEKADVWIDFSASEAFAAGLAAAHRAG
                     RAFLSGTTGLGREHQGSLDAAARDIPVLHAANMSFGMAVLDRLVAEAAKLLGEEFDAG
                     IFEMHHRGKKDAPSGSALALGATIAATRGRKPGAEFVRRDENVREPGGIGFASLRGGD
                     VVGDHTVIFAGEGERLELGLYVSERAVFARGALRAAAWLAHRPPGLYRMNDVIGL"
     gene            1072..1710
                     /locus_tag="VFX38_05965"
     CDS             1072..1710
                     /locus_tag="VFX38_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX5314434.1"
                     /translation="MKIAIIGAGNVGQALGFAWAKLGHEICFGVRDPESAQARALLQR
                     QAGLSVASNGAAAACSELVALATPWDATRAAVASCGDLAGKIVMDCTNPIKADLSGLA
                     LGCDTSGAEQIASWAPGAQLFKAMNQIGFNLMDQPHFAAGQPVMFVCGEGAAKAAVLN
                     LVATLGFEAVDAGGLAVARLLEPYAMLWIHLAITSKLGRDFAFSLLRPATPA"
     gene            1920..3023
                     /gene="carA"
                     /locus_tag="VFX38_05970"
     CDS             1920..3023
                     /gene="carA"
                     /locus_tag="VFX38_05970"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006788012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX5314435.1"
                     /translation="MPQTAILALEDGSIFRGVSFGAPGVAVGELVFNTAMSGYQEILT
                     DPSYARQLVCLTTAHVGNVGVNPEDIESGKVQAEALVVRSAGPGPSSWRARGALGEFL
                     AAAGRPAIEELDTRSLTRHLRAHGALNACLATDLDENEAVARACEFPGLLGMDLAAAV
                     SCAEAYDWRDGLWEKTPDPPRHRVVVYDFGVKRNILRALVDHGCELRVVPARTPAAEV
                     IAAGPDGVVLSNGPGDPAASSYGIEATRALLEARIPLLGICLGHQFLGLASGARTMKT
                     PFGHHGANHPVQELANGRVWITSQNHGFAVDGETLPANLQATHVSLFDGTLQGIARSD
                     TPAIAFQGHPEASPGPHDVLPMFERFVALMERR"
     gene            3023..>4959
                     /gene="carB"
                     /locus_tag="VFX38_05975"
     CDS             3023..>4959
                     /gene="carB"
                     /locus_tag="VFX38_05975"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX5314436.1"
                     /translation="MPKRTDIETILVIGAGPIVIGQACEFDYSGTQAVKALREEGYRV
                     VLVNSNPATIMTDPATADAIYIEPVHWKTIAGVIEKERPQALLPTMGGQTALNCALDL
                     VREGVLEKFGVELIGASRDAIDVAEDRERFREAMHEIGLATPRSGLAHSMEDALAVQA
                     EIGFPTIIRPSFTLGGSGGGIAYNRDEFVEICELGLDLSPTNELLIEESVLGWKEFEM
                     EVVRDRADNCIIVCAIENLDPMGVHTGDSITVAPAQTLTDKEYQRMRDAAIAVLRRVG
                     VETGGSNVQFAVDPETGRMFVIEMNPRVSRSSALASKATGFPIARVAAKLAVGYTLDE
                     LRNEITGGLIPASFEPAIDYVVTKLPRFNFEKFPGANDRLTTQMHSVGEAMGIGRSFA
                     ESLQKTLRSLETDCDGLDPMWREEDGPEARDRLLRELRQPGPLRLWYVADAFRLGYPF
                     EEIAATTRIDPWFLETIRRLVDEERAVAAGGLRALDRDRLYALKRLGFSDARLARLLG
                     CREDELRALRLRHGLRPVYKRVDSCAAEFPSATAYLYSTYEEECEAEPGERRKIMVLG
                     GGPNRIGQGIEFDYCCVQAVLALRAAGYETLMVNCNPETVSTDFDTADRLYFEPLTFE
                     DVIEIVELEKPAGVIVQFGGQT"
BASE COUNT          813 a         1618 c         1690 g          838 t
ORIGIN      
        1 tgcgcgtgcg cggcaagggt ttgccgcttc ccggcggcgg gcgcggggat ctcttctgcg
       61 cggtgcaggt gcagacgccc gtgaatctga acgaagagga aaaggagttg atccgcaatc
      121 tggacgagca gttgcgaatc ggcggctccc gccacaatcc ggccgaacag gaaagctgga
      181 ccgcgaaggt gaagaacttc ttcgagcagc tcgcgggatg aatgcgccga gcgtcaagtc
      241 cggcgagccc atccggattg cgttgctcgg ggcgacgggg cggatgggac gggagatcgt
      301 cgccgcgata cgtcaagcgc cggatctgcg tctcgtcgcc gcactcgctc gcgcggacga
      361 tgagagcctc ggccgcgatg cgggtgagat cgccggcggg gcggcgctcg gagtcgccct
      421 ctccggggac ctgaatgcgg cgtcggagaa ggccgatgtc tggatcgatt tttcggcatc
      481 ggaggcgttt gcggccggtc tcgccgccgc gcaccgcgcc ggccgcgctt tcctcagcgg
      541 cacgaccgga ctcgggcgcg agcatcaggg gtccctcgat gcggcggctc gtgacattcc
      601 cgtgctgcac gcggcgaaca tgagtttcgg catggcggtg ctcgatcgtc tcgtcgcgga
      661 agcggcgaaa ctcctcggcg aggaattcga tgccgggatt ttcgagatgc accatcgcgg
      721 caagaaggac gcgccctcgg gcagcgcgct cgcgcttggc gcaacgattg ccgcgactcg
      781 cgggcggaaa cccggcgcgg aattcgtccg gcgcgacgaa aatgtgcgcg aacccggcgg
      841 gatcggcttc gcgagccttc gcggcggcga tgtcgtcggc gatcacaccg tcatcttcgc
      901 gggagagggc gaacgcctgg agttgggcct ttacgtcagc gagcgtgccg tgttcgcccg
      961 cggcgccctg cgcgccgcgg cctggctcgc gcatcggccg ccgggcctct atcgcatgaa
     1021 cgacgtcatc ggcctgtagc cgagtttttt tcctcccgac cggagcacat catgaaaatc
     1081 gccatcatcg gcgccggcaa cgtcggtcag gcgttgggtt tcgcgtgggc gaagctcgga
     1141 catgagatct gtttcggcgt ccgcgatccc gaatcggcgc aggcgcgggc gctgttgcag
     1201 cgccaggccg ggctctcggt cgcatcgaac ggcgcggcgg ccgcatgctc ggagctggtc
     1261 gccctcgcga cgccctggga cgcgacccgg gcggccgtcg cctcctgcgg agatctcgcg
     1321 ggcaagatcg tgatggactg taccaatccg atcaaggccg acctgagcgg actcgcgctg
     1381 ggatgcgaca cctcgggcgc cgagcagatc gcctcctggg cgccgggcgc acagctgttc
     1441 aaggccatga accagatcgg tttcaatctc atggatcagc cccatttcgc cgccgggcag
     1501 ccggtgatgt tcgtgtgcgg cgaaggtgcc gccaaggccg cggtgctgaa cctcgtggcg
     1561 acgttgggat tcgaggccgt ggacgccggc ggactcgccg tcgcgcgcct gctcgaaccg
     1621 tatgccatgc tctggattca cctcgccatt accagcaagc ttggacgcga cttcgcgttt
     1681 tccctgttgc gccccgcaac accggcctga tccgccttcg cacatgattg cgggctgtct
     1741 tcgatcccgt tcggctcgtc ccccgggctc ccgcgcgatg caacggggca ggcattcacc
     1801 agtccgggcg agcacggccg tcgcgaggag atcacgaccg tccgcgcgtc ggcaagctga
     1861 taaaatgtcc cgggttcgcg cgcagcctgc gtgcggcccc tgaactcgac gggggtcccg
     1921 tgcctcagac cgcaattctc gccctggaag acggctcgat cttccgggga gtatccttcg
     1981 gcgcgcccgg cgtggcggtc ggcgaactgg tattcaacac cgcgatgagc gggtaccagg
     2041 aaatcctcac cgatccttcc tacgcccgcc agctcgtttg cctcactacg gcgcacgtcg
     2101 gaaacgtcgg cgtgaatccc gaggacatcg aatccggcaa ggtgcaggcc gaggcgctgg
     2161 tcgtgcgcag cgccgggccc ggaccctcga gttggcgcgc ccgcggggcg ctcggggaat
     2221 ttcttgccgc cgccggacgc ccggcgatcg aagaactcga cacgcgcagc ctgacccggc
     2281 atctgcgcgc gcatggcgcg ctcaacgcct gtctcgcgac ggatctcgac gaaaacgaag
     2341 ccgtcgctcg ggcctgcgag tttcccggcc ttctcgggat ggatctcgcg gccgcggtca
     2401 gttgcgccga ggcctacgac tggcgggacg gcctgtggga gaagacgccc gatccgccgc
     2461 gccatcgcgt cgtcgtgtac gacttcggag tcaagcgcaa catcctgcgc gcgctcgtgg
     2521 atcatggctg cgaactgcgg gtcgttcccg cgcgcacccc cgcggccgag gtgattgcgg
     2581 ccggaccgga cggcgtcgtg ctttcgaacg gtcccggcga tcccgcagcc tcgagctacg
     2641 gcatcgaggc gacgcgcgcg ctgctcgagg cgcggattcc gctcctcggc atctgcctcg
     2701 gccaccagtt tctgggtctt gcgagcggag cgcgcacgat gaagactccc ttcggccacc
     2761 acggcgccaa ccatccggtg caggaactcg cgaacgggcg agtgtggatc acgagccaga
     2821 atcacggatt cgccgtcgac ggcgagacgc ttcccgccaa tttgcaagcg acccacgttt
     2881 cgctcttcga cggaacgctg caggggatcg cgagaagcga cacgcccgcg attgcgtttc
     2941 agggacatcc ggaggcgagc ccggggcccc acgatgtgct ccccatgttc gagcgtttcg
     3001 ttgcgctcat ggagcggcgc tgatgccgaa gcgaaccgac atcgaaacga ttctcgtcat
     3061 cggcgccggg ccgatcgtga tcggccaggc ctgcgaattc gattattcgg gaacgcaggc
     3121 ggtaaaggcg ctccgtgaag aaggctatcg cgtggtgctg gtgaattcca acccggcgac
     3181 gatcatgacc gatccggcga cggccgatgc gatctacatc gaacccgtgc actggaagac
     3241 gatcgcgggc gtgatcgaga aagagcgccc gcaggcgctg ctgccgacca tgggcggaca
     3301 gaccgcgctc aactgcgcgc tcgatctggt gcgcgaaggc gtgctcgaga aattcggcgt
     3361 cgaactgatc ggcgcctcgc gcgacgcgat cgacgtggcc gaggatcgcg agcgatttcg
     3421 cgaggcgatg cacgagatcg gtctcgcgac cccgcgatcg gggctcgcgc acagcatgga
     3481 agatgcgctc gcggtgcagg cggagatcgg gttcccgacg atcattcgcc cgtcgttcac
     3541 gctcggcggc tcgggcggcg gcatcgcata caaccgcgac gaattcgtcg aaatctgcga
     3601 actcggcctc gacctttcgc ccaccaacga actgctgatc gaggagtcgg tgctcggctg
     3661 gaaggaattc gagatggagg tggtgcgcga tcgcgccgac aattgcatca tcgtttgcgc
     3721 gatcgaaaac ctcgatccga tgggggtgca cacgggagac tcgatcacgg tcgcgcccgc
     3781 gcagaccctg accgacaagg aatatcagcg catgcgcgat gcggcgatcg cggtgctgcg
     3841 ccgcgtcggg gtggaaacgg gcggctccaa cgtgcagttt gcggtggacc cggaaaccgg
     3901 acgcatgttc gtgatcgaga tgaatccgcg ggtttcgcgt tcctcggcgc tcgcctccaa
     3961 ggccacgggt ttcccgatcg cgcgcgtcgc ggcgaagctc gccgtcggct acacgctcga
     4021 cgagctgcgc aacgaaatca ccggagggct gattcccgct tccttcgagc cggcgatcga
     4081 ttacgtcgtc accaaactgc cgcgattcaa cttcgagaag tttcccggcg cgaacgatcg
     4141 gctgacgacg cagatgcatt cggtgggcga ggcgatgggg atcgggcgga gcttcgccga
     4201 atccctgcag aagaccctgc gcagcctcga aaccgattgc gacggcctgg atccgatgtg
     4261 gcgcgaggaa gacggccccg aggcgcgcga tcgcctgctg cgggaactgc gccaacccgg
     4321 gccgctgcgg ctgtggtatg tcgccgatgc atttcgtctc ggttacccgt tcgaagagat
     4381 cgcggcgacg acgcgcatcg atccctggtt tctcgagacg atacgccggc tcgtggacga
     4441 agagcgcgcg gtagccgcgg gggggctccg cgcgctcgat cgcgaccgcc tgtacgctct
     4501 caagcgcctt ggattctccg atgcccgcct tgcgcgtctc ctcggctgcc gcgaagacga
     4561 gctgcgcgcg ctgcgcctgc gtcacggtct tcgccccgtt tacaagcgcg tggattcctg
     4621 tgcggccgag tttccgtcgg caaccgccta tctctattcg acctatgaag aggagtgcga
     4681 agccgagccc ggcgagcgac gcaagatcat ggtcctcggc ggcgggccga accgcatcgg
     4741 ccagggcatc gagttcgatt actgctgcgt gcaggcggtg ctcgcgctca gggcggccgg
     4801 ttacgaaacg ctgatggtca attgcaatcc cgagacggtt tcgaccgatt tcgacaccgc
     4861 cgatcgcctg tacttcgagc cgctcacctt cgaggacgtg attgagatcg tcgagctgga
     4921 aaagcccgcc ggcgtcatcg tgcagttcgg cggccagac
//
LOCUS       DASUJG010000278         7726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_328009, whole genome shotgun sequence.
ACCESSION   DASUJG010000278 DASUJG010000000
VERSION     DASUJG010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7726
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328009"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /gene="rseP"
                     /locus_tag="VFX38_05980"
     CDS             <1..888
                     /gene="rseP"
                     /locus_tag="VFX38_05980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEX5314437.1"
                     /translation="PTWEAVQLALLGGVVSERELRLTVQAAAGPERRVRLRYDDPKAL
                     TQPGRLLPGLGLSVWLPPLPPVLGKVLPDGAAAQAGLMSGDRVVAINGKPVADWSTLT
                     KTVQANPGRRLLFTVERAGQHLTLPVVPASESGTGAPVGRIGVAQRVPPDYGANLRAE
                     NRLPPLAALQAGASRTGEMTVVTVVMLYRMVVGQASLRNLSGPISIAQYAGSWARAGV
                     VPFLFFLAVISISLGILNILPIPLLDGGQLLYLGFEAVRGRPLSPRAEALGQRVGLSL
                     IAVLVGFAIFSDLSRLFHP"
     gene            908..3301
                     /gene="bamA"
                     /locus_tag="VFX38_05985"
     CDS             908..3301
                     /gene="bamA"
                     /locus_tag="VFX38_05985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEX5314438.1"
                     /translation="MPGRHIAARVLVVLTLAACLGLPAARAADSGSFTVQNIRVIGLE
                     RISKATVLTYIPQISVGQTVGPADISKAITSLYQTGFFTQVQFRRDGSTLVIVVRERP
                     TIAEVALSGNKQLKTADLEKGLEQAGLTKGSFFNRAALEGIEGSLIQTYFDHGRYGVQ
                     VHSTVRPLPNNRVLIAIRIREGANAKVLAINFTGNKAFDAGTLRGRFKMSTPGWLTWI
                     TGKDKYAEEKLQGSLENLRSFYMDRGYADFRVNSVQVQISPDKSGIYINVNLHEGGKY
                     KIGSVKLLGEFPIAQEKLENLLFIKPGSTFSMQVANAQAQLLTNELGNFGYGFAEVNP
                     LPHPDPKTHKVELVFYVKPGQRVYVRHVVFSGAPGTNDSVFRREMRQAEGSWLNNFNL
                     QRSRVRIQRLPFVDTVDVKPVRVPGTSDQVDVNVNVKERQSGTANVFLAYSGLYGLGF
                     GGEVALSNLFGEGKLLHLNASKNSSITSVTADYTDPYATANGVSRTLSLFYSKGTSLN
                     INSSQFVSKNYGGGVTYGFPLSEFDSYSLGGSLRHGTLTPFCNSSLQFFDFVRNPANG
                     NVSFQQSYCGGLDPTVPVNVALPNLTYTNFVASAGYVHDTRNRTVLPSRGTLQQFSVN
                     LALPIGDETFYTASWNQFTFVPLGAGFIYGINSLVGIGGAYGKTSDLPPYERFFSGGP
                     DSVAGYEDGTLGPLDSNGLPYGGDFVTWVQNELVLPNFIGGPDVQNSYRFALFIDAGN
                     VFNRPSDFSFSYIRMSYGVGVTWLTPIGALHFSYAVPFHYRIGDNLARFQFTLGAYF"
     gene            3301..3837
                     /locus_tag="VFX38_05990"
     CDS             3301..3837
                     /locus_tag="VFX38_05990"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEX5314439.1"
                     /translation="MIRGASLLVVAALAALAPRIASAGSVAWVDYDALIKEAPQTQAS
                     NKLLTQEFFGRQLQIEKDRSTVKSLGAKLAAPETQTNVLERASVSEKLKAAKSALQSD
                     QSAYDTAQTMRQQQLLANLKDLIQQEIETYARAHDISVVLRDGELYAGKRTDITTQIL
                     ARLQYDYAQAQTASGKTP"
     gene            3834..4865
                     /gene="lpxD"
                     /locus_tag="VFX38_05995"
     CDS             3834..4865
                     /gene="lpxD"
                     /locus_tag="VFX38_05995"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEX5314440.1"
                     /translation="MSERGVSLGEIVATLGGELRGDPELRVDSVVAIAGAAPGGLGFL
                     ANARYRTQLATTRATCVVLRAEDAAACPTACIVTDDPYLYYARAAAMIVPPPAPCAGI
                     HPEATVAAGAAISPDAWIGPRAVIAARAQIGARVHVGPGCVIGEGVEIGAATRLSANV
                     TVLAGARIGRDCLLHPGVVIGADGFGIARDGDAWRKVPQLGSVVIGDRVEIGANTTID
                     RGTIEDTVIADGVKLDNLIQIGHNVRIGADTVIAASSGVSGTTVIGARCMIAGQVGIA
                     GHLEIADDTIVTGKTFVNHSIHAPGRYSGALPMDESSRWRRNSARFRSLDEIERRLAR
                     LERSEKGIE"
     gene            4865..5323
                     /gene="fabZ"
                     /locus_tag="VFX38_06000"
     CDS             4865..5323
                     /gene="fabZ"
                     /locus_tag="VFX38_06000"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEX5314441.1"
                     /translation="MDGTGGFDIQRILELLPHRYPFLFVDRVLECVPGERLVAIKNVS
                     IDEPFFVGHFPEKPVMPGALLLEAMAQTCSVLISITHAVTPGTLYYLVRVDRAKFRRP
                     VEPGDQLLLSAAPRRRIRNMDCFDARAEVDGKLAAEAQIWCAPQKVVDST"
     gene            5383..5805
                     /gene="fabZ"
                     /locus_tag="VFX38_06005"
     CDS             5383..5805
                     /gene="fabZ"
                     /locus_tag="VFX38_06005"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005220378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEX5314442.1"
                     /translation="MDIHDILERLPHRYPFLLVDRVVECVPDERLVALKSVTMNEPFF
                     VGHFPIRPVMPGVLVLEAMVQTTSLLVRESHYDSDEGLHYLVGVDKASFRRPVEPGDQ
                     LRLTAVLDKVVRKIGRFQTRAEVDGRLVASAQLLCASR"
     gene            5809..6579
                     /gene="lpxA"
                     /locus_tag="VFX38_06010"
     CDS             5809..6579
                     /gene="lpxA"
                     /locus_tag="VFX38_06010"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEX5314443.1"
                     /translation="MIDSRALIHPGAQIAEGVRVGAFAILGENVRIGAGTTIGAHTII
                     EGPSEIGEGNRIFPFVSLGLEPQDKKYGGEETALKIGNGNTIREFCTINRGTAQHRNV
                     TEIGDDNWIMAYVHIAHDCVIGDHTILANNVTLGGHVSIGDWAVLGGFAKVHQFCRLG
                     SHCFAQMDAGIAKDVPPYVMAAGMPARPRGVNREGLRRRGFTPAAIRAIVEAYRLIYR
                     SGHLLADARGELALRAGTQPALVPLVEFLAASERSIIR"
     gene            6625..>7726
                     /gene="lpxB"
                     /locus_tag="VFX38_06015"
     CDS             6625..>7726
                     /gene="lpxB"
                     /locus_tag="VFX38_06015"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEX5314444.1"
                     /translation="MPLRIALIAGEASGDQLGAHLIAALRARENAVEVMGVAGAAMRA
                     AGCEPLADVDDMSVMGFAELAIHLRRLLALRRRLVRELSARKPDLVVGIDVPDFNLGL
                     ERRLRERGLATAHYVSPSVWAWRPGRVHAVARAAECVLCLLPFEPQCYAAADVKAVFV
                     GHPLVDALRARPREDARPALGLATGPVLAILPGSRAVEVKQLAGTFVAAAAALRRMQP
                     ELQVVIPLARPSLRPGIEAAIAKTGLAGATLLDGQAHAAISAADVVLSASGTATLETL
                     LLERPMVVAYRVQAFTAWMLRRPGVLRTRCFSLPNLIAERKIVPELLQEEASAAKISA
                     TLRLLLDNPEARNAQLAAFDSVREKLGSDAAGR"
BASE COUNT         1310 a         2512 c         2502 g         1402 t
ORIGIN      
        1 ccgacctggg aagcggtgca gctcgcgctt ctcggcgggg tggtgtcgga acgcgagttg
       61 cgcctgaccg tgcaggcggc ggcaggaccc gagcgcaggg tgcgcctgcg ctacgacgat
      121 cccaaggcgc tcacgcagcc cggcaggctt ctccccgggt tgggcttgag cgtgtggctg
      181 ccgcccctgc cgccggtatt gggcaaggtt ttgccggacg gcgcggccgc gcaggcaggg
      241 ctgatgagcg gcgatcgcgt agtcgcgatc aacggcaagc cggtcgcgga ctggtcgacg
      301 ctcacgaaga cggtgcaggc gaaccccggc cgtcgcctgc tgttcaccgt cgagcgggca
      361 gggcagcacc tcacccttcc cgtcgtcccc gcttccgagt cggggacggg cgctccggtc
      421 gggcgcatcg gcgttgccca gcgcgttcct ccggactacg gagcaaatct gcgcgcggag
      481 aaccgcctgc cgcctctcgc cgccctccag gctggggcct cgcgcaccgg agaaatgacg
      541 gtcgtgaccg tcgtcatgct ctaccgcatg gtggtcggcc aagcctcgct ccggaatctc
      601 agcggcccga tttcgattgc gcaatacgcc ggttcctggg cgcgtgcggg ggtggttccg
      661 ttcctgtttt tccttgccgt aatcagtatc agcctcggca tcctcaacat tctgcccatc
      721 ccgcttctcg acggcggaca gcttctctac ctcggtttcg aagcggtccg cggtcgtccc
      781 ttgtcgcccc gggcggaggc gctcggacag agggtgggtt tgagtttgat cgcagtgctg
      841 gtaggctttg ccatcttcag cgacctcagc cgtctcttcc atccctagct tcccgaggag
      901 ggccaccttg cccggacgcc acatcgcagc ccgagttctc gttgtcctga cgctggccgc
      961 gtgtctgggg ctgccggccg cccgtgccgc ggacagcggt tccttcacgg tccagaacat
     1021 tcgagtgatc ggcctcgagc gcatttccaa ggcaaccgtt ctcacctaca ttccgcagat
     1081 cagcgtcggg cagacggtgg ggccggcgga tatctccaag gcgatcacga gcctctatca
     1141 gacgggattc ttcacccagg tgcaattccg gcgcgacggc tcgaccctgg tcatcgtcgt
     1201 tcgcgagcgg ccgacgatcg ccgaggtcgc cctcagcggc aacaagcagt tgaagacggc
     1261 ggatctcgag aagggcctgg agcaggccgg cctcaccaag ggcagcttct tcaaccgcgc
     1321 cgcgctcgag ggaatcgaag gctcgctcat ccagacctat ttcgaccacg gccgctacgg
     1381 cgtccaggtg cactccaccg tccggccgct tcccaacaat cgcgttctga tcgcgatccg
     1441 gatccgcgag ggcgcgaacg ccaaggtgct cgccatcaac ttcaccggca acaaggcgtt
     1501 cgacgcggga acgctgcgag gccgcttcaa gatgtcgact cccggctggc tcacctggat
     1561 caccggcaag gacaagtacg cggaggaaaa actccagggc agcctcgaga atctgcgctc
     1621 gttctacatg gatcgcggct acgccgactt tcgcgtgaat tccgtccagg tgcagatttc
     1681 acccgacaaa tcgggcatct acatcaacgt caatctccac gagggcggca agtacaagat
     1741 cggctccgtg aaacttctcg gagagtttcc gatcgcgcag gagaagctcg aaaatctctt
     1801 gttcatcaaa cccggcagca ccttctccat gcaggtcgcc aacgcgcagg cgcagctgct
     1861 gaccaacgaa ctcgggaact tcgggtacgg attcgcggaa gtgaacccct tgccgcatcc
     1921 cgatccgaag acccacaagg tggagctggt cttttacgtc aagcccggcc agcgcgtgta
     1981 tgtccgtcat gtcgtcttca gcggcgcgcc ggggaccaac gattcggtgt tccgtcgcga
     2041 gatgcgtcag gccgagggca gctggctcaa caatttcaac ctgcagcgct cgcgggtgcg
     2101 catccagcgt ctgccgttcg tcgataccgt cgatgtgaaa cccgttcggg ttccgggcac
     2161 ctccgaccag gtggacgtga acgtcaacgt gaaggaacgc cagtcgggaa cggcgaatgt
     2221 ctttctcgcc tattccggac tgtacggcct gggtttcggc ggtgaggtgg ctctctcgaa
     2281 tcttttcggc gagggcaagc tgctgcatct gaacgcgagc aagaacagca gcatcacgag
     2341 cgttacggcc gactacaccg atccctatgc gaccgccaac ggcgtgagtc gtacgctgtc
     2401 cttgttctac tccaagggaa cgagtctcaa catcaattcg tcgcagttcg tctcgaagaa
     2461 ctacgggggc ggggtgacct acgggttccc gctctccgaa ttcgattcct acagcctcgg
     2521 cggaagtctg cgtcacggca cgctcacgcc attctgcaat tcttcgttgc agttcttcga
     2581 ctttgtgcgc aaccccgcga atggaaacgt cagttttcag caatcctatt gcggggggct
     2641 cgacccgacc gtaccggtga acgtcgcgct gcccaatctg acctacacca acttcgtcgc
     2701 ctcggcgggc tacgtgcacg atacccgcaa tcgcacggtg cttccgagcc gcggaacgct
     2761 ccagcagttc agcgtcaatc tcgccctgcc gatcggagac gagacgttct acacggcttc
     2821 ctggaatcag ttcaccttcg ttccgctcgg cgcgggattc atctacggga tcaacagcct
     2881 cgtcggaatc ggcggggcct acggcaaaac ctccgacctg ccgccctacg aacgattctt
     2941 ctcgggtggt cccgattcgg tggcgggtta cgaggacggc accctcgggc cgctcgattc
     3001 caacgggctt ccctatggcg gagacttcgt cacctgggta cagaacgagt tggtgctccc
     3061 gaatttcatc ggcgggccgg acgtgcagaa ttcctaccgc ttcgccctgt tcatcgatgc
     3121 gggcaacgtc ttcaaccgac cgagcgattt cagtttcagc tacatccgga tgtcctacgg
     3181 agtcggcgtg acctggctca cgccgatcgg tgcgctgcac ttcagttacg cggttccctt
     3241 ccattaccgc atcggcgaca atctcgcgcg gttccagttc acgctcggcg cctacttctg
     3301 atgattcgcg gcgcaagtct gctggtcgtc gccgcgcttg cggcgctggc cccgcggatc
     3361 gcgtcagcgg gttccgttgc ctgggtcgat tatgacgcgc tcatcaagga ggcgccgcag
     3421 acgcaagctt cgaacaagct tttgacgcag gaattttttg gacgacaact tcagatcgag
     3481 aaagatcgca gcacggtgaa gtcgctcggt gcgaaacttg ccgcacccga gacgcagacg
     3541 aacgtgttgg agcgggcgag cgtgagcgag aaattgaagg cagcgaaaag cgccttgcaa
     3601 agcgatcagt ccgcctatga cacggcgcag accatgcgcc agcagcaact gctcgcgaat
     3661 ctgaaggacc tgatccagca ggagatcgaa acgtacgccc gcgctcacga tatttccgtg
     3721 gttctgcgcg acggcgaact gtacgcggga aagcgaaccg acatcaccac gcagattctg
     3781 gcgcgtctgc agtacgacta cgcgcaggcg cagaccgcga gcggcaagac gccgtgagcg
     3841 aacgcggcgt cagcctcggc gagatcgtgg cgacgctcgg aggcgaactt cgcggcgacc
     3901 ccgagctgcg ggtggattcg gtcgttgcga tcgccggggc ggcaccgggg gggctcggct
     3961 tccttgcgaa cgcgcgctac cggacgcagc tcgccacgac gcgcgcgacc tgcgtcgttc
     4021 ttcgggccga agacgcggcg gcttgtccga cggcctgcat cgtcaccgac gatccgtacc
     4081 tgtattacgc ccgcgcggcg gcgatgatcg tcccgccgcc ggcgccttgc gccggaatcc
     4141 atccggaggc gacggtcgcg gcgggcgccg ccatcagccc ggatgcctgg atcggaccgc
     4201 gggccgtgat cgcggcgcgg gcgcaaatcg gcgcgcgagt tcatgtcggc ccgggctgcg
     4261 tgatcggcga aggagtcgag atcggcgccg cgactcgcct gagcgcgaac gtcaccgtac
     4321 tcgcgggcgc gcgcatcggg cgcgattgtc tgctgcatcc cggcgtcgtc atcggcgccg
     4381 acggcttcgg aatcgcccgc gacggcgacg cctggcgcaa ggtgccgcaa ctcggaagcg
     4441 tcgttatcgg cgatcgcgtg gaaatcggcg caaacacgac catcgaccgc ggtaccatcg
     4501 aggacacggt catcgccgac ggtgtcaagc tcgacaatct catccagatc ggacacaacg
     4561 tgcgcatcgg cgccgacacc gtgatcgccg cctcctccgg ggtctccggc accaccgtca
     4621 tcggtgcgcg ctgcatgatt gcggggcagg tcgggatcgc gggtcatctg gaaatcgcgg
     4681 atgatactat tgtcaccgga aaaaccttcg tcaatcattc cattcacgcg ccggggcgtt
     4741 actccggggc gctgccgatg gatgagagtt cgcggtggcg gcgcaacagt gcgcgcttcc
     4801 gttcgctcga cgaaatcgaa cggcggctcg cacggctgga gcgcagcgaa aaggggatcg
     4861 agtagtggac ggtaccggcg gtttcgacat ccagcggatt ctcgagttgt tgccgcatcg
     4921 ctatcccttc ctgtttgtgg accgggtgct cgagtgcgtg ccgggcgaac gtctcgtcgc
     4981 gatcaagaac gtgagcatcg acgaaccgtt tttcgtcggt cactttccgg aaaagccggt
     5041 gatgcccggc gcgctgctgc tggaggcgat ggcccagacg tgcagcgtgc tgatcagcat
     5101 cacgcatgcc gttacgccgg gaacgctgta ctatctcgtg cgcgtggatc gcgcgaaatt
     5161 ccgccgcccg gtcgagcccg gagatcagct tcttctgagc gccgcgccgc gtcgccgcat
     5221 ccgcaacatg gattgtttcg acgcgcgcgc cgaagtggac ggcaaactgg cggccgaggc
     5281 gcagatctgg tgcgcgccgc agaaggtcgt cgactcaacc tgactcgaac cccgctgccg
     5341 ccggcggtcg cctcgcgggc gggggggaaa gaagtgagac gcatggatat tcacgacatt
     5401 ctggaacgat tgccccaccg ctatcctttc cttctggtgg accgcgtggt cgaatgcgtc
     5461 ccggacgagc gactggtcgc gctgaagagc gtcacgatga acgagccgtt tttcgtggga
     5521 cattttccga ttcgcccggt gatgcccggg gtgctggtcc tggaggcgat ggtgcagacg
     5581 acgagtctcc tcgtgcgcga gagtcactac gattccgacg aaggactgca ctatctcgtc
     5641 ggcgtcgaca aggcgagctt tcgccgtccg gtggagccgg gcgaccagct ccggcttacc
     5701 gccgtgctcg acaaggtggt gcgaaaaatc ggccgcttcc agacccgcgc ggaagtggac
     5761 ggacgcctgg ttgcctcggc gcaacttctg tgcgcctcga ggtgaggcgt gattgattcg
     5821 cgggcgctga ttcacccggg cgcgcaaatc gccgagggag tgcgggtcgg agccttcgcg
     5881 atcctggggg agaacgtccg catcggagcc ggtaccacga tcggggcgca cacgatcatc
     5941 gagggtccct ccgagatcgg cgagggaaac cgcatttttc cgttcgtgtc gctcgggctc
     6001 gaaccgcagg acaaaaaata cgggggcgag gagaccgcgc tcaaaatcgg gaacggcaac
     6061 accatccgcg agttctgcac catcaatcgc ggcacggcgc agcatcgcaa cgtgaccgaa
     6121 atcggcgacg acaactggat catggcctac gtccacatcg cgcacgactg cgtgatcggc
     6181 gatcacacga tcctcgccaa caacgtcacg ttgggagggc acgtcagcat cggcgattgg
     6241 gcggtgctcg gggggttcgc caaggtacac cagttctgcc ggctcggatc ccattgtttc
     6301 gcgcagatgg atgccggcat tgcgaaggac gtccctccct acgtgatggc ggcgggcatg
     6361 ccggcgcgcc cgcgcggagt caaccgcgag ggcctgcggc ggcgcggatt cacgcccgcc
     6421 gcgattcgcg ccatcgtcga ggcgtatcgc ctgatctacc gctccggtca tctgctcgcg
     6481 gacgcgcgcg gcgaactcgc gctgcgagcc ggaacccagc cggcgctggt gccgctggtc
     6541 gagtttctcg cggccagcga gcgcagcatc attcgctgat gagggtggcc gccgctcgaa
     6601 tcgcatccgt tcccacgccg cgccatgcct cttagaatcg cactcatcgc cggggaggca
     6661 tccggagatc agctcggcgc ccatctcatc gcagctctgc gcgcccgcga gaacgcggtc
     6721 gaagtgatgg gcgtcgccgg tgcggccatg cgcgccgccg gctgcgagcc gctcgcggac
     6781 gtggacgaca tgtccgtcat ggggtttgcg gaactcgcga tccatttgcg tcggctgctg
     6841 gcattgcgcc ggcgcctggt ccgcgaactc tcggcgcgga agcccgatct ggttgtcggc
     6901 atcgacgttc ccgatttcaa tctggggctg gagcgcagat tgcgtgagcg cggcctcgcg
     6961 accgctcact acgtcagtcc ctcggtgtgg gcctggcgtc cgggacgcgt gcatgccgtc
     7021 gccagggccg ccgaatgcgt gctctgtttg ctgccgttcg aaccgcagtg ctacgccgcg
     7081 gccgatgtca aggccgtgtt tgtggggcac ccgctggtcg atgcgctccg tgcacgtccg
     7141 cgggaagacg cgcgccctgc actcggactg gccacgggac ccgtgctcgc gattttgccg
     7201 ggctcgcggg cggtcgaggt aaagcaactc gcggggacgt tcgtggcggc ggcggccgcg
     7261 ctccgccgca tgcaaccgga gttgcaggtc gtgattccgc ttgcgcgccc gagcttgcgc
     7321 ccggggatcg aggccgcgat cgcaaagacc gggctcgccg gcgccacgct gctcgacggg
     7381 caggcgcatg ccgccatctc ggcggcagat gtcgtcctca gtgcctccgg aacggcgacc
     7441 ctcgagaccc tgctcctcga gcgcccgatg gtggtcgcct accgcgtgca ggcgttcacc
     7501 gcctggatgc tgcgccgccc gggagtgctt cgaacgcgct gcttttcctt gccgaatctc
     7561 attgcggagc gcaagatcgt tccggaattg ctgcaggagg aagcaagcgc ggcgaaaatt
     7621 tccgcgacgc tgcgcctgct tctcgataat ccggaggcgc gcaatgccca actggcggcc
     7681 ttcgattcgg tgcgggaaaa actggggagc gatgctgccg gtcgtg
//
LOCUS       DASUJG010000279         2396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1061816, whole genome shotgun sequence.
ACCESSION   DASUJG010000279 DASUJG010000000
VERSION     DASUJG010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061816"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="VFX38_06020"
     CDS             <1..853
                     /locus_tag="VFX38_06020"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX5314445.1"
                     /translation="VLRVGARPILVDVDLASRNLDAARVEAALTERTRAVMPVHFAGL
                     AVDMDPLNDLAAKRGLHVVEDAAHAIGTRCRGRLIGSFGELACFSFHANKNLTTIEGG
                     AIVTADERALTALRALRFHGQVKLEDGGYDVVSAASKYNMSDVCARIGLDQLAVLEAR
                     NARRRELAGLYFEALAELDELLLPARGDEGHAWHMFAVCIPFAKLGTDRKRLREALQE
                     RGIGTGVHYPALHGLSICRGMGLRAEDFPNAERIGRETLTLPLFPEMSDGDVERVAAA
                     LRGVMHG"
     gene            846..1802
                     /locus_tag="VFX38_06025"
     CDS             846..1802
                     /locus_tag="VFX38_06025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5314446.1"
                     /translation="MAETPRVSVVVPVYNEEAGLEALFARLYPALDALGVPYEVLFVD
                     DGSRDRSAAILRAQYQKRPEVTRVILLQANFGQHAAICAAFAKARGERTVTLDADLQN
                     PPEEIGNLLAAMDAGHDYVGTIRRQRRDRWWRRSASRLMNRIREKITRVHMTDQGCML
                     RAYSRNVVDAINDTREIKTFIPALAYLYASNPVEIEVGHDERAAGASKYSLWRLMQLN
                     FDLVTGFSIAPLQIFALFGALVALGSLALFVWLMVDRFVHGPDVNGVFTLFAIAFFFI
                     GVLLFGIGILGEYIGRIYQEVQRRPRYLIRGTLGDDDESRRE"
     gene            1799..>2396
                     /locus_tag="VFX38_06030"
     CDS             1799..>2396
                     /locus_tag="VFX38_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEX5314447.1"
                     /translation="MSATQRARAVVFAYHDVGVRGLEVLLARGVEVPLVVTHEDDPAE
                     AGWFASVADHARRYGIETVTPAQAELPVLEPRLAALAPDFLFSFYYRHLLPADLIATA
                     RRGALNLHGSLLPRYRGRAPVNWAVLAGETETGASLHYMTAKADAGDLVAQESVPILP
                     NDTAAEVMTRIAVTGERLLWRLLPELIAGTAPRKPLDLA"
BASE COUNT          373 a          804 c          791 g          428 t
ORIGIN      
        1 cgtgcttcgc gtcggcgcgc gcccgattct cgtcgacgtg gatctcgcga gccgcaacct
       61 cgatgcggcg cgggtcgagg cggcgctcac ggagcgcacg cgcgccgtga tgccggtgca
      121 tttcgccggg ctcgccgtgg acatggaccc gctcaacgat ctcgcggcga agcgcgggct
      181 gcacgtcgtc gaggacgcgg cgcacgccat cggcacccgt tgtcgcgggc gcctcatcgg
      241 gagcttcggc gaactcgcct gcttcagttt ccacgcgaac aagaatctca cgacgatcga
      301 gggcggcgcg atcgtgaccg ccgacgagcg ggcgctcact gccctgcggg cgctgcgttt
      361 ccacgggcag gtaaagctcg aagacggagg gtacgacgtc gtttccgccg cgagcaaata
      421 caacatgagc gatgtctgcg cgcgcatcgg cctggatcag ctcgcggtgc tcgaagccag
      481 aaatgcacgt cgccgcgaac tcgccggtct ctatttcgag gcgctcgcgg aactcgacga
      541 acttctcctt cccgcacgcg gcgacgaggg tcacgcctgg cacatgttcg cggtctgcat
      601 tcccttcgcg aagctcggca ccgaccgcaa gcgtttgcgc gaggcgctgc aggagcgcgg
      661 catcggaacg ggcgtgcatt atccggctct ccatgggctc agcatctgtc gcgggatggg
      721 actgcgcgcc gaagactttc cgaacgcgga gcggatcggg cgcgaaaccc tgacgcttcc
      781 gctctttccc gaaatgagcg atggcgatgt cgagcgggtc gccgcggcgc ttcgcggcgt
      841 gatgcatggc tgagacgccg cgggtctcgg tcgtcgtccc cgtttacaac gaggaagcgg
      901 ggctcgaggc gctgttcgcg cggctctatc cggcgctcga cgcgctcggc gtcccgtacg
      961 aggtgctctt cgtggacgac ggcagccgcg accgcagcgc cgcgattctg cgcgcacagt
     1021 atcagaagcg cccggaagtt acccgcgtca tcctgctgca ggcgaacttc ggccagcatg
     1081 ccgcgatctg cgccgccttc gccaaggccc gcggcgagcg cacggtcacg ctcgacgccg
     1141 atctccagaa tccgcccgag gaaatcggga atcttctcgc ggcgatggac gcggggcacg
     1201 actacgtggg cacgattcgc cgtcagcgcc gcgatcgctg gtggcgccgt tccgcctcgc
     1261 gtctcatgaa tcgcatccgc gaaaagatta ctcgcgtgca catgaccgac cagggttgca
     1321 tgctgcgcgc ctattcccgc aacgtcgtgg acgcgatcaa cgacacccgc gagatcaaga
     1381 ccttcattcc cgcgctcgcc tatctctatg cctccaatcc ggtggagatc gaggtcgggc
     1441 acgacgagcg cgccgccggg gcatccaagt attcgctgtg gcgcctgatg cagctcaatt
     1501 tcgatctcgt caccggcttt tcgatcgcgc cgttgcagat attcgcgctt ttcggcgcgc
     1561 tcgtcgcgct cggctcgctc gcgctcttcg tctggctgat ggtcgatcgc ttcgtccatg
     1621 ggccggacgt gaacggggtg ttcacgctct tcgcgatcgc gtttttcttc atcggcgtgc
     1681 tgctcttcgg catcggaatt ctcggcgaat acatcgggcg catctaccag gaggtgcagc
     1741 ggcggccgcg ttatctgatc cgcggaacgc tcggggacga cgacgaaagc aggcgcgaat
     1801 gagcgcgacg cagcgagccc gcgccgtcgt cttcgcctat cacgatgtcg gcgtgcgcgg
     1861 cctcgaagtg ctgcttgccc gcggcgtcga agtaccgctc gtcgtcaccc acgaggacga
     1921 tcctgccgaa gccggctggt tcgcgagcgt cgcggatcat gcccgccgct acggcatcga
     1981 gacggtcacg ccggcgcagg ctgaattgcc agttcttgag ccccggcttg cggcgctcgc
     2041 gccggacttt ctcttttcgt tctattaccg ccatcttctg cccgcggacc tgatcgcaac
     2101 ggcgcggcgc ggtgcgctca atctgcacgg atcgctgctg ccgcgctatc gcggacgggc
     2161 gcccgtgaac tgggcagtgc tcgcaggcga gaccgaaacc ggcgcgagcc tgcattacat
     2221 gacggcgaag gcggacgccg gtgatctcgt cgcccaggag tcggtgccaa tccttcccaa
     2281 cgatacggcc gcggaagtga tgaccaggat cgcggtgacg ggggaacggc tgctatggcg
     2341 tctgctgccg gaactgatcg cgggcacggc gccgcggaaa ccgctcgatc tcgcgg
//
LOCUS       DASUJG010000280         4707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_450340, whole genome shotgun sequence.
ACCESSION   DASUJG010000280 DASUJG010000000
VERSION     DASUJG010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450340"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VFX38_06035"
     CDS             complement(<1..587)
                     /locus_tag="VFX38_06035"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5314448.1"
                     /translation="MSTAARESAVRLRNIYSHFGRHVVHDHIDLDIARGEIVALLGGS
                     GSGKTTLLRTMALLHRPQQGEVELFGQRVQPDSVAELILRKRMGYMFQFGALFGGMTV
                     LRNVELPLIEHTTLPARLREEVSQLKIQLTGLEAAAAGLMPSELSGGMRKRAALARAL
                     ALDPELLLLDEPGSGLDPASARSLDELIGRLRAALG"
     gene            complement(584..1708)
                     /locus_tag="VFX38_06040"
     CDS             complement(584..1708)
                     /locus_tag="VFX38_06040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HEX5314449.1"
                     /translation="MARKQEPDRPQIKLDGHVIHLAGSWTSVTLVRALAQIPRRIPPR
                     GLLTIDLAGVSALDTAGAWFIQRSVRNLRHDGRQVEFANLNANASSLLELVSEQTDPR
                     DVKLVHPHFTILHRTGYATIQLGEVLVSYLGFMGETAERWVRIVGNTRRWRIPQFFGI
                     VEETGVTAVPIIALLSFLIGVVIAYEGLFVLRSYGASLFVVNLAGVTVLRELGPLIAA
                     IVVAGRTGSAFAAQIGTMKVSEEVDAMVAMGLSPAEILVLPRIFGLMLALPLLTVLAD
                     LAGLVGALVTASLVSGISAHAFFAQLASAVSPATVLAGLAKTPVFALVIATVGCFHGF
                     KAESSAESVGARTTRSVVQSIFFVIVIDAVFAVAYSSLGI"
     gene            1925..2000
                     /locus_tag="VFX38_06045"
     tRNA            1925..2000
                     /locus_tag="VFX38_06045"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1958..1960,aa:Phe,seq:gaa)
     gene            complement(1979..2707)
                     /locus_tag="VFX38_06050"
     CDS             complement(1979..2707)
                     /locus_tag="VFX38_06050"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019659789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX5314450.1"
                     /translation="MTERHAAPAPARPLPRDFYARGAVAVARALLGKLLVREYRGERL
                     SGIISEVEGYLGERDSASHAFRGPTPRNAVMFGEGGHIYVYFVYGMHHCMNVVAGREG
                     RAAAVLLRGIRPLEGIATMRRLRMGREPLADGPGKLCQALAIDRSLDGHDLTRGEKLW
                     IESRPAPSRSAIGASPRIGIAYALPEHRDAKWRFFLREGRCVCGGVEPLRPLARAVMV
                     TTAGFANPGATPRRRREDGGQGRN"
     gene            complement(2700..3230)
                     /locus_tag="VFX38_06055"
     CDS             complement(2700..3230)
                     /locus_tag="VFX38_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009286961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEX5314451.1"
                     /translation="MNQEPGDVEIEIVRGDIARQAGIDAVVNAANAALLPGGGVAGAI
                     HRAAGAGLAAECGPLAPIAPGEAVVTDAHDLPNRWVIHCLGPVYGRDEPAAGLLADCY
                     RNALARAEEKGAVSIAFPAISTGAFGYPAQAAAEVCARAIREAVPSLENIRLVRFVLF
                     DAAMKRIVERAFAARD"
     gene            3388..>4707
                     /locus_tag="VFX38_06060"
     CDS             3388..>4707
                     /locus_tag="VFX38_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314452.1"
                     /translation="MRESDGRVHKVATAAGGRAAIGRAVAAFGGALLLGASSFGVPAL
                     AAAPRLPCPSQWTQIPTEDPPNGNNPSASAVSFGSQSDGWLVGGQTNGNNVILPFAEY
                     WNADDQSWHFGNPLLPPGAHGDVRLGGVISFAASEDQPAEAWGVGHQAGGLLLEHWLG
                     PDPGSPWQVATPASLPPGSAFLTAVAGASRNDLWAVGYSFVDSSKNGTSTLSALIARY
                     DGSSWNVVPAPALGDDIVGSGLIDVSAASASDVWAVGYQTDGEGYRTLIEHYDGNAWS
                     AVSDSPNPGSAYDVLLGVWAAAGDDVWAVGYRSSGTSYSPLVLHYDGSHWNVIANTPG
                     ATLQSGFRSVAEVASSGQVAAAGFDFSADAGNYIPFSIAWPKSQPDDTTVLPPAASAG
                     RTIGSEFRDLTVVPGADRLWTVGEPAVVETLCLGEAAPAPKVAATSMQ"
BASE COUNT          796 a         1583 c         1578 g          750 t
ORIGIN      
        1 ccgagcgcgg cgcgcagccg cccgatcagt tcgtcgagcg agcgggcgct cgccggatcg
       61 agccccgaac ccggctcgtc gagaaggaga agctccggat cgagcgcgag cgcgcgggcg
      121 agggcggcgc gcttgcgcat cccgcccgaa agctcgctcg gcatgaggcc cgccgcggcc
      181 gcctcgaggc ccgtcagttg aatcttgagc tgcgagactt cctcgcgcag tcgcgccgga
      241 agcgtcgtgt gttcgatcag cggcagttcc acgttgcgca gcaccgtcat gccgccgaag
      301 agcgctccga actggaacat gtagcccatg cgctttcgca ggatgagttc cgccacggaa
      361 tccggctgaa cccgttgccc gaagagttcc acctccccct gttgcgggcg gtggagcagc
      421 gccatcgtgc gcagcagcgt cgtttttccg ctgccgctgc cgccgagaag cgcgacgatc
      481 tcgccgcgcg cgatgtccag gtcgatgtga tcgtgcacga cgtgacggcc gaagtggctg
      541 tagatgttgc gcagccgcac cgccgattcc ctggctgcgg tgctcatatt ccgagcgagc
      601 tgtaggcgac ggcgaatacg gcatcgatga cgatgacgaa gaagatgctc tgcaccacgc
      661 tgcgggtggt acgcgcgccg acgctctcgg cgctgctctc cgccttgaag ccgtggaaac
      721 agccgacggt cgcgatgacc agcgcaaaca ccggcgtctt ggcgagcccg gcgagcaccg
      781 tcgccggcga caccgcgctc gccagttgcg cgaagaacgc atgcgcggaa atgccggaaa
      841 cgaggctcgc cgtgaccagc gcgccgacga gtccggcaag atcggcgagc acggtcagga
      901 gcggaagcgc gagcatgaga ccgaagatgc gcggcaagac cagaatctcg gcggggctga
      961 gccccatcgc gaccatcgcg tccacttcct cggaaacctt catggtgccg atctgcgcgg
     1021 caaaggcgga tccggtgcgt ccggcgacca cgatcgcggc gatgaggggg ccgagttcgc
     1081 gcagcaccgt tacgcctgcg agattgacga cgaagaggct cgcgccgtag ctgcgcagca
     1141 cgaagagccc ttcgtaggcg atgacgacgc cgatcagaaa ggaaaggagc gcgatgatcg
     1201 gcaccgcggt gacgccggtt tcctcgacga ttccgaagaa ctgcggaatg cgccagcggc
     1261 gcgtgttccc cacgatccgc acccagcgct ccgccgtctc gcccatgaag ccgagatagc
     1321 tgacgagcac ctctcccaac tggatcgtcg catatccggt acggtgcaga atcgtgaaat
     1381 gcggatgcac cagcttcacg tcgcggggat cggtctgctc ggagacgagt tccagcaggc
     1441 tgctcgcgtt ggcgttgagg ttcgcgaact ccacctggcg tccgtcatgg cgaagattgc
     1501 gcaccgaacg ctggatgaac caggcgccgg cggtatcgag cgcgctcacg ccggcgagat
     1561 cgatggtgag aaggccgcgc ggcgggatgc gccgcgggat ctgggcgagc gcgcgcacca
     1621 aggtgacgga agtccagctt ccggcgaggt ggatgacatg cccatcgagc ttgatctggg
     1681 gacgatccgg ttcctgtttt ctcgccaccg cggctcccga ttgcggccga tttgcgctcc
     1741 tcgcccggaa gtctcgcgcg cgccacttcc gtccgcgctc attgtagccg cgcgccgcgc
     1801 cgcgatggat acacggcgcc gacttcgcga tcgtcgcgct tgacgccgag gcgtcgcggc
     1861 cggtctaata agcaccgcag cagggcggcg gggaagcctg cggcgattcc ccgttcccaa
     1921 gggcggccag gtagctcagt cggtagagca gaggactgaa aatcctcgtg tcggcggttc
     1981 aattccgtcc ctggccacca tcttcgcgcc gtctccgcgg cgtggccccg gggttcgcaa
     2041 atcccgccgt cgtcaccatc accgcccggg cgagtggccg caaaggctcc actccgccgc
     2101 agacacagcg cccctcgcga agaaagaaac gccatttcgc atcgcggtgc tccggcagtg
     2161 cataggcgat gccgatgcgc ggactcgccc cgattgcact tcgcgacggc gccggccgcg
     2221 attcgatcca gagcttctcg ccgcgggtga gatcgtgtcc gtcgaggctg cgatcgatcg
     2281 cgagcgcctg acagagcttc cccggcccgt cggcaagcgg ctcgcgaccc atgcgcagcc
     2341 ggcgcatcgt cgcgatgcct tcgagcgggc gaatgccgcg cagaagaacg gcagcggccc
     2401 ggccttcccg accggcgacg acgttcatgc agtgatgcat gccgtagacg aagtacacgt
     2461 agatgtgtcc gccctcgccg aacatcaccg cattgcgcgg agtcggcccg cgaaaggcgt
     2521 gacttgcgct gtcgcgctct ccgagatagc cctcgacctc actgatgatg ccgctcaggc
     2581 gctccccgcg gtattcgcgc acgagcagct tcccgagaag cgcacgggcg accgcaaccg
     2641 ccccgcgcgc atagaaatcg cgcggcaggg gcctcgccgg agcgggagcg gcgtggcgct
     2701 cagtcacgtg cggcgaaggc gcgttcgacg atgcgcttca tcgcggcgtc gaaaagaacg
     2761 aagcggacaa gtctgatgtt ttcgagcgag ggaaccgctt cgcggatcgc tcgggcgcag
     2821 acttccgccg cggcctgcgc gggatagccg aaggcgccgg tcgaaatcgc cggaaacgca
     2881 atgctcaccg cgcccttttc ctcggcacgt gcaagcgcgt tgcgataaca atcggcgagc
     2941 agtcccgccg ccggttcgtc gcgcccgtag accgggccga gacaatgaat cacccaacga
     3001 ttgggaagat cgtgggcgtc cgtcacgacc gcctcgcccg gagcaatcgg cgcgagcggg
     3061 ccgcattcgg cggcgagccc cgctccggcc gcgcgatgaa tcgccccggc gacgccgccg
     3121 cccggaagca gtgcggcgtt ggccgcgttg acgacggcat cgattccggc ctgacgcgca
     3181 atgtcaccgc gaacgatttc gatctcgaca tcgcccggtt cctgattcat ctcgcgcgtc
     3241 ggcatcggca atcgaaacac gatacaccgc agcactcccg cagccgcgga cggatcgcgt
     3301 cgaagtttct cgcgccgagc gtgacggcag aggcttatgc cacaatgacg cggttttcac
     3361 catgcggctc gcggaaggaa tgccttcatg agggaatcgg acggccgggt tcacaaggtg
     3421 gcgacggccg caggcggccg cgctgcgatc gggcgcgccg tggccgcatt cggcggcgcg
     3481 ctgctcctcg gcgcctcgtc gttcggcgtg ccggcgctcg ccgcggcgcc ccgcctgccc
     3541 tgcccgtcgc agtggacgca aatcccgacg gaggatccgc cgaacggcaa caatccatcg
     3601 gcctccgccg tttctttcgg ctcgcagagc gacggatggc tggttggcgg gcagaccaac
     3661 ggcaacaatg tgattttacc gttcgccgaa tactggaacg ccgacgatca gtcctggcat
     3721 ttcgggaatc ccttgttgcc cccgggggcg catggagacg tgcggctggg gggagtgatt
     3781 tcctttgcag cttcggaaga ccagccggcc gaggcctggg gcgtcggcca tcaggcaggc
     3841 gggctgctcc tggagcactg gctgggaccg gatccaggct ctccgtggca agtcgctact
     3901 cctgcctcgt tgccgccggg ctctgccttt ctcaccgccg tcgcgggcgc gagccgcaac
     3961 gatctctggg ccgtcggcta cagttttgtc gattcgagca agaacgggac ttccacgctc
     4021 tcggcgctga tcgcgcgcta cgacggctcg tcgtggaacg tcgtcccggc acccgcgctc
     4081 ggcgatgaca tcgtcggcag cggtctcatc gatgtatcgg ccgcaagtgc gagcgatgtc
     4141 tgggctgtcg gctatcagac cgacggcgag ggctatcgca ccctgatcga acattacgac
     4201 ggcaacgcct ggtcggcggt gtccgacagt cccaacccgg gaagcgcata cgacgttctc
     4261 ctcggagtgt gggcggccgc gggcgacgac gtctgggcgg tcggctatcg atcgagcgga
     4321 acaagttatt cgccgctcgt tctccattac gacggcagcc attggaacgt gattgccaat
     4381 actcccggcg ccacgctcca gagcggattt cgcagcgtcg ccgaggtcgc ctccagcggt
     4441 caggtggcgg cggccggttt cgatttctcg gcggacgcag gcaactacat tcccttttcc
     4501 atcgcctggc cgaagtcgca acccgatgac acgacggtgc tgccgccggc tgcgagcgcg
     4561 ggccggacaa tcgggagcga attccgcgac ctcacggtcg tcccgggcgc cgatcggttg
     4621 tggacggtag gcgaacccgc cgtggtggag acgctgtgcc tcggcgaggc cgcacctgcg
     4681 cccaaagttg cggccacttc gatgcag
//
LOCUS       DASUJG010000281         3690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_83826, whole genome shotgun sequence.
ACCESSION   DASUJG010000281 DASUJG010000000
VERSION     DASUJG010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83826"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1043
                     /locus_tag="VFX38_06065"
     CDS             <1..1043
                     /locus_tag="VFX38_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA-associated domain-containing protein"
                     /protein_id="HEX5314453.1"
                     /translation="PWLDVLENVRLGLEAQGIEPAEQHRRALAAIDMIGLDGFESAYP
                     KELSGGMRQRVGFARALVVEPDVLLMDEPFSALDVPTAETLRDDLLDLWREKKIPTRA
                     ILMVSHSIEESLLLADRLLILDGNPASIKAELPVRLAHPRDRNAPAFRGLVDRVYAAM
                     TRPATAFLGAPPTAEIGLGYRLPHANVQQMLGVLDAVCEESQGKRLELAELADELEME
                     VDEIFPPIEALELLGFATLDEGAVHLTASGHAFLEADILQRKVLFGGVLMERVPLARR
                     IRSEIESEADHRVDEERFLVALNESLSDEEAERVLEVVVAWGRYAEIFAYDYDSGIFS
                     LENPSTEEVGKA"
     gene            complement(1267..1485)
                     /gene="infA"
                     /locus_tag="VFX38_06070"
     CDS             complement(1267..1485)
                     /gene="infA"
                     /locus_tag="VFX38_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX5314454.1"
                     /translation="MPKEESLQMEGRVVETLPNTTFRVELENGHVVTAHISGRMRKNY
                     IRILTGDKVTVELTPYDLTKGRIVFRGR"
     gene            complement(1508..2215)
                     /gene="aat"
                     /locus_tag="VFX38_06075"
     CDS             complement(1508..2215)
                     /gene="aat"
                     /locus_tag="VFX38_06075"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEX5314455.1"
                     /translation="MRPELIAGAPQDWFPPDTAALAEPNGLLAVGGDLSAARLLAAYR
                     RGIFPWYEEGQPILWWTPEPRCVLDPRELRVSHRLRRTLRQARFTVSWNGDFGGVIRG
                     CAAPRASGEGTWITPAMIAAYERLHRLGHAHSIEIHRGGHLVGGLYGIALGAAFFAES
                     MFSRERDASKFALVALCARFAGIRESLIDCQIPSPHVIGLGAKLLPRSEFLRDLARAI
                     AAAGPFDLPSGPSLGKI"
     gene            complement(2280..3407)
                     /locus_tag="VFX38_06080"
     CDS             complement(2280..3407)
                     /locus_tag="VFX38_06080"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5314456.1"
                     /translation="MHTSITEIPAKVWNALAGTAAPCLRHEYFAALEASRSVGPGSGW
                     IANYFTLADRRGKILGAVPLFEKRDSSGEFVFDWNWAAALERIGRNYYPKLVAAIPFT
                     PVPGPRLLLARPGDAALAAGLARAVQSFAAERGVSSLHWLFVAEEELPAFEAGHCLIR
                     SGCHFLWSNPGVANFEEWLATLTAARRKVIRRERRRIAEAGIRCEWRRGAELNAEELA
                     LIYRLYAANYYAHGMPPYLTPAFFVEFAQRLPDALIVCLARRGAETVAGAICLRDEER
                     LYGRYWGAFEWVDSLHFEVCYYQGIEYALAQGLRYFHPGVQGEHKLFRGFAPTLHYSA
                     HWLRDGDLRAAVAHFLDRERAAVGSYAAQAGEFLPFRHDKG"
     gene            complement(3426..>3690)
                     /locus_tag="VFX38_06085"
     CDS             complement(3426..>3690)
                     /locus_tag="VFX38_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004156891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5314457.1"
                     /translation="LAVAGVFVAIGHSPNTAIFEGRLKMQNGYIVVKGGRHGDATATS
                     VPGVFAAGDVMDHVYRQAITSAGSGCMAALDAEKYLEARGLGG"
BASE COUNT          616 a         1219 c         1251 g          604 t
ORIGIN      
        1 tcccgtggct ggacgtgctg gagaatgtgc ggctcggcct cgaagcgcag gggatcgaac
       61 ccgcggagca gcaccgccgc gcgctcgcgg caatcgacat gatcggcctc gacgggttcg
      121 agtccgccta cccgaaggag ctgtccggag gcatgcgcca gcgggtcggt ttcgcccgcg
      181 ccctggtcgt ggagcccgac gtgctcctga tggacgagcc gttctcggcc ctcgacgtgc
      241 cgacggcgga aaccctgcgc gacgatcttc tcgatctgtg gcgggagaaa aagatcccga
      301 cgcgcgcgat cctcatggtt tcgcacagca tcgaggaatc gctgctgctc gccgatcggc
      361 tgctgatcct cgacggcaat ccggcttcga tcaaggcgga actcccggtg cgtctcgcgc
      421 acccgcgcga tcgcaacgcc cctgccttcc gcggcctcgt ggaccgcgtc tacgccgcaa
      481 tgacgcggcc ggccacggcc tttctcggcg caccgccgac ggcggaaatc gggctcggct
      541 accgcctgcc gcatgccaac gtgcaacaga tgctcggggt cctcgatgcg gtctgcgagg
      601 agtcgcaggg caagcggctc gaactggcgg aactcgccga cgaactcgag atggaggtgg
      661 acgaaatctt cccgccgatc gaggcgctgg aactcctcgg cttcgcgacc ctcgacgagg
      721 gcgcggtgca tctcacggca tcggggcacg ccttcctcga agcggacatc ctccagcgca
      781 aggttctttt cggcggcgtg ctgatggagc gtgtgccgct cgcccggcgc atccgctccg
      841 agatcgaatc cgaggccgat caccgcgtgg acgaggagcg attcctcgtc gcgctcaatg
      901 agtcgctgag cgacgaggag gccgagcggg tgctcgaagt cgtggtcgcc tggggtcgct
      961 acgccgagat ctttgcctac gactacgaca gcggaatctt cagcctcgaa aatccctcga
     1021 ccgaagaagt gggcaaggcg taaagtcgct cactccgttc gctgcaagtc gctcgctgcg
     1081 cttgctgtca gccggccgct gtgcggcctg tgagaaagtc agaggaaggc cggcataata
     1141 ccgcaactcg ttgccggcaa tgcccgcggg aatgtgggct tcgccgaggc tgctatttta
     1201 tccgcttaca gtctgcgaag cggacgactt gcttcccgac ttccttgcgg gcgacgcagc
     1261 cgccgctcaa cggccgcgaa agacgatgcg ccccttggtg agatcgtagg gcgtgagttc
     1321 caccgttacc ttgtcgccgg tgaggatgcg gatgtaattc ttgcgcatcc gcccggaaat
     1381 atgggccgtc accacgtggc cgttctcgag ttcgactcga aacgtggtgt tcggcagggt
     1441 ctcgacgacc cggccttcca tttgcagact ttcttccttg ggcatgcgcg gcggcggcgg
     1501 tgaatcgcta aattttgcct aaacttgggc ccgatggcaa atcgaagggg cccgcggccg
     1561 cgatggcgcg cgccagatcc cggagaaatt cactgcgggg gagaagcttc gcgccgagtc
     1621 cgattacatg cggcgaggga atctggcagt cgatcaggga ctcgcggatt ccggcgaatc
     1681 gcgcgcagag tgcgaccagc gcgaacttcg aggcgtcgcg ctcgcggctg aacatggatt
     1741 cggcgaagaa ggccgcgccg agggcgatgc catagaggcc tccgaccagg tgcccgccgc
     1801 ggtggatttc gatcgaatgc gcatgaccga gccggtgcag acgttcatac gcagcgatca
     1861 tcgccggcgt gatccaggtt ccctcgccgg atgcgcgcgg cgcggcgcaa ccgcggatga
     1921 cgccgccgaa gtctccgttc caggagacgg tgaatcgcgc ctgccgcagc gttcgtcgca
     1981 ggcgatgcga gacgcgcagt tctcgcggat cgagaacgca gcgcggctcc ggcgtccacc
     2041 agagaatcgg ctggccttct tcgtaccagg gaaagattcc gcgccggtag gctgcaagca
     2101 gccgcgccgc ggagagatcg ccgccgaccg cgaggagacc gttgggctcg gcgagtgccg
     2161 ccgtgtccgg tggaaaccaa tcctgagggg cgccggcgat caattcgggg cgcatgccga
     2221 tccctcgaaa tccctgctgc cttggcgcgc gtggcgccga ttttccctcc gccacgggtt
     2281 cagcccttgt cgtggcgaaa gggcagaaat tcgccggcct gcgcggcgta gcttccgacg
     2341 gcggcgcgtt cgcgatcgag aaaatgggcg acggcggctc gcaggtcgcc gtcgcgcagc
     2401 cagtgggccg aataatggag cgtcggggcg aagccgcgaa agagtttgtg ttctccctgg
     2461 acgcccggat ggaaataccg caggccctgc gcgagcgcgt actcgattcc ctggtagtag
     2521 cagacctcga agtgcaggct gtccacccac tcgaaggcgc cccagtagcg cccgtagagc
     2581 cgctcctcgt cgcgcaggca gatcgcgccg gcaacggttt ctgcgccgcg gcgcgcgaga
     2641 cagacgatca gggcatcggg cagccgctgc gcgaactcga cgaagaaggc cggcgtgagg
     2701 tacggcggca tgccgtgggc gtagtaattc gccgcgtaga gccgatagat cagggcgagt
     2761 tcctccgcgt tcagttcggc tccgcggcgc cattcgcagc ggatgcccgc ctcggcgatc
     2821 cggcggcgct cgcggcggat gaccttgcgc cgcgccgccg tcagcgtcgc cagccactcc
     2881 tcgaagttcg cgaccccggg attgctccag agaaaatggc agccgctgcg gatcaggcaa
     2941 tgcccggctt cgaaggccgg cagttcctcc tcggcgacga acagccagtg cagggaggaa
     3001 acgccgcgtt cggcggcgaa gctctgcacc gcgcgggcga gccccgccgc cagcgcggcg
     3061 tcgccggggc gagcgagcag cagccgcggc cccggcaccg gcgtgaacgg gatcgcggcc
     3121 acgagcttcg gatagtagtt gcgcccgatg cgctccagcg cggccgccca gttccagtcg
     3181 aaaacgaact ccccgctcga atcccgtttc tcgaagagcg gcaccgcgcc caagatcttg
     3241 cccctgcggt ccgcgagcgt gaaataattc gcaatccagc ccgagcccgg accgacggag
     3301 cggctcgcct cgagcgccgc gaaatattcg tggcgcaggc agggcgccgc ggttcccgcg
     3361 agcgcgttcc agaccttcgc ggggatctcc gtgatcgagg tgtggatccg ctctttcgta
     3421 agcccttacc ctccgaggcc gcgggcctcg aggtatttct cggcgtcgag tgcggccatg
     3481 cagccgctgc cggcgctggt gatcgcctgg cggtagacgt gatccatcac gtcgccggcg
     3541 gcgaaaacgc ccggcacgct ggtcgcggtc gcgtctccgt ggcgcccgcc tttcaccacg
     3601 atgtagccgt tctgcatctt gagccgaccc tcgaagatcg ccgtgttcgg gctgtggccg
     3661 atggcgacga agacaccggc gaccgcgaga
//
LOCUS       DASUJG010000282         3683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_450474, whole genome shotgun sequence.
ACCESSION   DASUJG010000282 DASUJG010000000
VERSION     DASUJG010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450474"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..640
                     /locus_tag="VFX38_06090"
     CDS             194..640
                     /locus_tag="VFX38_06090"
                     /inference="COORDINATES: protein motif:HMM:NF018552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 domain-containing protein"
                     /protein_id="HEX5314458.1"
                     /translation="MLALTETLLPPDLLRPRSLAMLIGIYECNYRRLIELLGEPFDLP
                     PCSRLRLRGRPLLYIDRRAQSRYTLELRLTHLFAGERLPDLRVRLYRDARLAEALAPA
                     PCAGNDPLADLPARWRANLLLYKWLEYCLDSRRKANCPAPALARAH"
     gene            708..1283
                     /locus_tag="VFX38_06095"
     CDS             708..1283
                     /locus_tag="VFX38_06095"
                     /inference="COORDINATES: protein motif:HMM:NF019081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HEX5314459.1"
                     /translation="MKRRHGMMWVAAFAFAPALAFAEGSGLDFNVNGHAVRAAYDSEV
                     TLSGLDFSVEALHNSDNGNVADVGLGLRSNANPGASPVTALIGVKALWVDPSYTGVSA
                     GYGVALGGGVDFSLPSYNRIAFGVALYWAPSVTSFSNLDHFIEEEARAGYRVLPNGTV
                     YLGYRHMTAAFTNTDDLVIDNGLNVGMALRF"
     gene            1296..2039
                     /locus_tag="VFX38_06100"
     CDS             1296..2039
                     /locus_tag="VFX38_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018168501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX5314460.1"
                     /translation="MSGHSKWANIQHRKNAQDARRGRVFTRLIREVTTAARIGGADPA
                     TNPRLRLAFERANAANVPKDNLERALKRASGDADGQTIEEVGYEGYAPGGVAVLVECM
                     TDNRNRTVADVRHAFSRYGGNLGADGSVAYLFNRVGLLAIGAEANEERLLEAALEAGA
                     EDVAHDDEGGFEVLTDPNDFEAVRDRLRAGGFALAEAEITMRPASQVELDAPTAGRVL
                     ALIDALDELEDTQQVYTNARFPAEALAER"
     gene            2057..2557
                     /gene="ruvC"
                     /locus_tag="VFX38_06105"
     CDS             2057..2557
                     /gene="ruvC"
                     /locus_tag="VFX38_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006746019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX5314461.1"
                     /translation="MVPLVILGIDPGSRITGYGLIAVEGNGRSVLDYGVVRAPPVELG
                     RRIRAIYEGVAAVVARHAPDEIAIEQAFVHLNPAVALALGQARGAALAAALAIERPLA
                     EYAPRAVKLAIVGSGKAEKIQVAHMIRHLLRIAGPIPEDASDALAIALCHAHSRGLAA
                     RTAARA"
     gene            2554..3129
                     /gene="ruvA"
                     /locus_tag="VFX38_06110"
     CDS             2554..3129
                     /gene="ruvA"
                     /locus_tag="VFX38_06110"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX5314462.1"
                     /translation="MIGYLRGRLAERRPPHLVVEVGGIGYELEAPLSTFAVLPAGAGE
                     VQLYTHLMIREDTQQLFGFASEEERSLFRALLRASGVGARLALAILSGMSVEEFARTV
                     AERDFATLRRLPGIGQKTAERLVVEMADRIEGIRGPRAGGVLAEAGAALAALGYKPRE
                     VGAMLSGAQAPGRTSEELVREALRHSLGTKA"
     gene            3144..>3683
                     /gene="ruvB"
                     /locus_tag="VFX38_06115"
     CDS             3144..>3683
                     /gene="ruvB"
                     /locus_tag="VFX38_06115"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX5314463.1"
                     /translation="MDMRAEAGELREEPRLRPQRLAEYIGQDQVRERMEIFISAARGR
                     GETLDHCLLFGPPGLGKTTLAHIIAHEMGANLHQTSGPVLERAGDLAAILTNLERGDV
                     LFVDEIHRLSPVVEEALYPALEDFVLDIMIGEGPAARSSRLDLAPFTLVGATTRAGLL
                     TSPLRDRFGIVSRLDFYDPG"
BASE COUNT          567 a         1233 c         1250 g          633 t
ORIGIN      
        1 gtcggtggag gaaatcgccg ccaatagtct ctatcagcgg gagcttacgc cctcgctgct
       61 gtagggttcg ccggcgggcc accggttcgg ctcgccggct ttgtcacgcc cttgcatcgc
      121 cgggggcgcc ggggtgaaat ccgccggctg cgctgcttgc ggtgtactgt gccctacgtg
      181 ctaaggtaac gtcatgctcg ctttgaccga aaccctgctg cctcccgatc tgctgcgccc
      241 gcggagtctc gccatgctga tcggaatcta cgagtgcaat tatcggcgcc tgatcgagtt
      301 gctcggcgag cccttcgatc tgcccccgtg cagcaggttg cgtctgcgcg gcaggcccct
      361 gctgtacatc gatcgacgcg cgcaaagtcg ctatacgctc gaactgcgac tcacgcatct
      421 ctttgccggc gagcgcctgc cggatttgcg ggtacgtctg taccgcgatg cgcgcctcgc
      481 cgaggcgctc gctcccgcgc cctgcgccgg aaatgacccg ctggcggatc tgcccgcgcg
      541 ctggcgcgcg aacctgctgc tttacaagtg gctcgagtat tgtctcgact cccggcgcaa
      601 ggcgaattgc cccgcgccgg cgctcgcgcg ggcgcactga cggtcatcgc gcgttgcctc
      661 gcagtcatgg catcatatag cctgatcggg caggcacgga gcgcgaaatg aaacgacgcc
      721 atgggatgat gtgggtcgcc gcctttgcat ttgcgccggc tttggcgttt gccgagggca
      781 gcggcctcga tttcaacgtc aacggtcatg cggtgcgcgc ggcctacgat tccgaagtca
      841 ccctctcggg gctcgatttt tccgtcgaag cgttgcacaa ttccgacaac ggaaacgtcg
      901 cggatgtcgg cctcgggctg cgttcgaatg ccaacccggg agcctcgccg gtcacggcac
      961 tgatcggcgt gaaagcgctg tgggtagacc cgagctacac gggggtgtcg gccggctacg
     1021 gggtcgcact cggcggcgga gtggatttct cgctcccgag ctacaaccgc atcgccttcg
     1081 gcgtcgcgct ctattgggcg ccctcggtta cgagtttcag caacctcgac cacttcatcg
     1141 aggaagaggc gcgggcgggc taccgggtac tgccgaacgg aacggtctat ctcggctatc
     1201 gacacatgac cgcggccttc accaataccg acgatctcgt gatcgacaac ggcctcaatg
     1261 tgggcatggc actgcgcttc tgagcgggag tcgcgatgtc cgggcacagc aagtgggcga
     1321 acatccaaca tcgcaagaac gcgcaggatg cgcgtcgcgg gcgggttttc acgcgtctga
     1381 ttcgtgaggt cacgacggcg gcgcgcatcg ggggcgccga tcccgcaacc aatccgcgcc
     1441 tgcgtctcgc cttcgagcgg gcgaatgccg ccaacgtccc gaaggacaat ctcgagcgcg
     1501 cgctcaagcg cgcgagcggg gacgcggacg ggcagacgat cgaggaggtc ggctacgaag
     1561 gatacgcgcc gggcggtgtc gccgtgctcg tcgaatgcat gaccgacaat cgcaatcgca
     1621 ccgtcgccga cgtgcgtcat gccttcagcc gttacggcgg caatctcggc gccgatggct
     1681 cggtcgcgta tttgttcaat cgcgtcggcc tactcgccat cggcgcggag gcgaacgagg
     1741 agcgtctgct cgaagccgcg ctcgaagcgg gcgcggaaga cgtcgcgcac gatgacgagg
     1801 gcggcttcga ggtccttacc gaccccaacg atttcgaagc ggttcgcgat cggctgcgcg
     1861 cgggcggctt cgcgctggcg gaggccgaga tcacgatgcg tccggcgagc caggtggaac
     1921 tggatgcacc gaccgccgga cgggtgctgg ctctcatcga tgccctggac gaactcgagg
     1981 atacgcagca ggtgtatacc aacgcccgct ttcccgccga ggcgcttgcc gagcgctgag
     2041 gcagatcgaa cgacgcgtgg tgccgcttgt cattctgggg atcgatccgg gttcgcggat
     2101 taccggctac ggactgatcg cagtggaggg aaatgggcgt tccgttctcg attacggcgt
     2161 ggtgcgcgcg ccgcccgtcg aactcggccg gcgcatccgc gcgatctacg aaggggtcgc
     2221 cgccgtggtc gcgcgccatg cgcccgacga aatcgcgatc gagcaggcct tcgtgcactt
     2281 gaatcccgcg gtcgcactcg cgctcggcca ggcgcgcggg gccgcgctcg ccgccgcgct
     2341 cgcgatcgag cggcctctcg ccgagtacgc gccgcgtgcg gtcaaactcg cgatcgtcgg
     2401 ctccggcaag gcggaaaaga tccaggtcgc gcacatgatc cgccatctgc tgcgcatcgc
     2461 cgggccgatt cccgaggacg cgagcgacgc gctcgcgatc gcgctttgcc acgcgcacag
     2521 ccgcggcctt gccgcgcgca cggcggcgcg cgcatgatcg gatatctgcg cgggcgtctc
     2581 gccgagcgcc gcccgccgca tctcgtggtg gaggtaggcg gaatcggcta cgaactcgag
     2641 gcaccgcttt cgaccttcgc cgtgctgccc gcgggcgcgg gcgaagtcca gctctacacg
     2701 catctgatga ttcgcgaaga cacgcagcag ctcttcggct tcgcgagcga ggaggagcgc
     2761 agcctctttc gcgcgctcct gcgtgcgagc ggagtcggcg ccaggctcgc gctcgcgata
     2821 ctgtccggaa tgtcggtcga ggaattcgcg cgcaccgtcg ccgagcgcga cttcgcgact
     2881 ctccggcgcc tccccggaat cgggcagaag accgccgagc gtctggtcgt cgaaatggcc
     2941 gatcgcatcg aaggaattcg cggcccgcgt gccggcggcg tgctcgcgga ggccggtgcc
     3001 gcgctcgcgg cgctcggcta caagcctcgc gaggtcggcg ccatgctctc cggcgcgcag
     3061 gctcccgggc gcacgagcga agaactcgtg cgcgaggcgc tgcgccattc actgggaacg
     3121 aaagcgtgag cggcgagcgc ctgatggaca tgcgcgcgga ggccggcgag ctgcgcgagg
     3181 aaccgcgcct gcgcccgcag cgcctggcgg agtacatcgg ccaggatcag gtgcgcgagc
     3241 gcatggagat tttcatttcg gcggcgcgcg ggcgcggcga gacgctcgat cattgccttc
     3301 tgttcggccc gcccggcctc ggcaagacga cgctcgcgca catcatcgcc catgagatgg
     3361 gggccaatct tcatcagact tcggggccgg tgctcgaacg cgccggcgac ctcgcggcga
     3421 tcctcaccaa tctcgaacgc ggcgacgtgc tcttcgtcga cgagattcat cgcctcagcc
     3481 cggtcgtgga agaagcgttg tatcccgcgc tcgaggattt cgtcctcgac atcatgatcg
     3541 gcgaggggcc cgccgcgcgt tccagtcgcc tcgatctcgc gccctttacg ctcgtgggcg
     3601 ctacgacgcg ggccgggctt ctgacctcgc cgctgcgcga tcgcttcggc atcgtctcgc
     3661 ggctcgactt ctacgatccg ggg
//
LOCUS       DASUJG010000283         2010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1184236, whole genome shotgun sequence.
ACCESSION   DASUJG010000283 DASUJG010000000
VERSION     DASUJG010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184236"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VFX38_06120"
     CDS             <1..304
                     /locus_tag="VFX38_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEX5314464.1"
                     /translation="GLIDDFGDLGAAVRSAASLAKLDNYGVTYVEPQLSPGEQFLVKL
                     AGDGSVRAVSRHLFAGGSDLVATLRPVWEPLARALALDDPNGVYAYCFCAAATTLR"
     gene            325..1932
                     /locus_tag="VFX38_06125"
     CDS             325..1932
                     /locus_tag="VFX38_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0009250 - glucan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein G"
                     /protein_id="HEX5314465.1"
                     /translation="MENEDRRGRRFGRIIGLPALAVLVALGVWLMPFEAHAFGFDDVV
                     AQAKALAAKPWQPVAAIPDWLQYPALDYDQYQQIRFRPDKRLWRGDGSGFNVMFLPAG
                     LYYTRPVTIRVVNDGVSRPFAFDKSLFTYPGKSFAKKVPSDLGYGGVVLTYPLERGAA
                     YNSFLVFAGASYFRAVATGENFGLSARGLAVDTGLATPESFPFFRALWLVKPAPGADT
                     MDLYALLDGEHASGAYHFTVHPGAPTVLDVTAVLFFRDHPARVGIAPLTSMFYYGANT
                     PRPPGEWRRAVHDSSGLGVENGDGEWLWRPLINPDTVTTTSFTVDNPAGFGLLQRPRD
                     FRDYEDLEARYDLRPSAWVAPQGSWGKGEIMLVELPESNETEDNIVAFYTPDTLPSAG
                     EPLRLQYRLSFGDENVGAPPAGYAAATYVGAGNNPGTQASGCTLRFIVDFRGGELANL
                     SEGVEPEVATVAGGDIEEVHVEPAPVFSGWRLSFLVSPVKGQPLDLRAYLRTDKTTLS
                     ETWTYMLPTDMLSAYQPACKDPQDPGR"
BASE COUNT          312 a          674 c          644 g          380 t
ORIGIN      
        1 cggtctgatc gatgacttcg gcgatctcgg tgccgcggtg cgttccgccg cctcgctcgc
       61 gaaactggat aactacggcg ttacctatgt cgagccgcaa ctgtctccgg gcgaacagtt
      121 cctcgtgaag cttgcgggcg acggcagcgt gcgggcagtc tcccggcatc tgttcgccgg
      181 cggaagcgat ctcgtcgcaa cgctgcgccc cgtgtgggag ccgctcgcgc gcgcgctggc
      241 gctcgacgat ccgaacggcg tttacgccta ctgcttctgt gccgccgcga ccacgctgcg
      301 ttgagccgac gcgaagcgac agccatggaa aacgaggatc ggcgcggcag gcgttttggc
      361 aggatcatcg gactgccggc cctcgctgtc ctcgtcgcgc tgggagtctg gctgatgcct
      421 ttcgaggcgc atgccttcgg cttcgatgac gtggtcgcac aggccaaggc gctcgcagcg
      481 aagccctggc agccggtcgc cgcgatcccc gactggttgc aatacccggc gctcgactac
      541 gatcagtacc agcaaattcg tttccgtccc gacaagcgtc tgtggcgagg cgacgggagc
      601 ggcttcaacg tgatgttcct gccggcgggc ctttattaca ctcgacccgt gacgattcgc
      661 gtcgtgaacg acggcgtatc tcgccccttc gccttcgaca agtcgctctt cacctatccc
      721 ggcaagtcgt ttgcgaaaaa ggttccgtcg gacctcggct acggcggcgt cgttctcacg
      781 tatccgctcg agcggggcgc ggcatacaat tcgtttctcg tgttcgccgg cgcgagttat
      841 ttccgcgctg ttgcgaccgg cgagaacttc gggctgtcgg cgcgcgggct cgccgtcgat
      901 accggtctcg ccacgcccga gtcgtttccg ttctttcgtg ctttgtggct ggtgaaaccg
      961 gcgcccggcg ccgacaccat ggatctctac gcacttctcg acggcgaaca cgcgagcggc
     1021 gcctaccact tcacggtgca tccgggcgca ccgactgtgc tcgacgttac cgccgtgctc
     1081 ttctttcgcg atcatcccgc gcgcgtcggg atcgcgcctc tcacgagcat gttctactac
     1141 ggtgccaaca cgccgcgccc gccgggggaa tggcgtcgcg ccgtgcacga ttcgagcggg
     1201 ctcggcgtcg aaaacggtga cggcgagtgg ttgtggcgcc cgctcatcaa tccggacacg
     1261 gtgaccacga cgagcttcac cgtggacaat ccggccggct tcggcctgtt gcagcgtccc
     1321 cgcgactttc gcgattacga ggatctcgag gctcgctacg atctgcgccc gagcgcctgg
     1381 gtcgcgcctc aggggagctg gggcaaggga gaaatcatgc tggtcgagct gcccgaaagc
     1441 aacgagaccg aggacaacat cgtggctttc tacacgcccg acacgctgcc gtctgcgggc
     1501 gaaccgctcc ggttgcagta ccgcctgagc ttcggcgacg agaacgtggg cgcaccgcct
     1561 gccgggtacg cggccgcgac ctatgtcggg gcgggaaaca atccgggcac gcaggcgagc
     1621 ggctgcaccc tgcgtttcat cgtcgacttt cgcggcggcg aactcgcgaa tctgagcgag
     1681 ggagtcgagc ccgaggtcgc gacggtcgcc ggcggcgaca tcgaagaggt gcatgtggag
     1741 cccgcgccgg tcttctcggg ctggcgattg agctttctcg tttcgcccgt caagggacag
     1801 ccgctcgatc tgcgtgccta cctgcgcacc gacaagacga cgctcagcga aacctggact
     1861 tacatgttgc cgaccgacat gctctccgcg tatcaacccg cctgcaagga ccctcaggat
     1921 cccgggcgct gacgcatcca gcgttcgctc aggatgcgct tgccgtagtg cagcgaggcg
     1981 gtgatttctt cgtcgccgcc accaccgctc
//
LOCUS       DASUJG010000284         3045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_816266, whole genome shotgun sequence.
ACCESSION   DASUJG010000284 DASUJG010000000
VERSION     DASUJG010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816266"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..478)
                     /locus_tag="VFX38_06130"
     CDS             complement(164..478)
                     /locus_tag="VFX38_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020680409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5314466.1"
                     /translation="MRNLFSPLPASRDGEMVEALVETGAFRLERIVSLGQSTDWLEQD
                     RREWVLLVSGAARLEFADGAVVALGPGDCLDIPAHFRHRVQWTDPERPSLWLALHYLE
                     DG"
     gene            complement(486..932)
                     /locus_tag="VFX38_06135"
     CDS             complement(486..932)
                     /locus_tag="VFX38_06135"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XamI family restriction endonuclease"
                     /protein_id="HEX5314467.1"
                     /translation="MSARRNTSKEAQEKAVKDVLGSAGMEEVKARDIPILTAAPPPGR
                     FCGETRLAGTRADVVARLRDGRIMTIECKVSNSTVNSYKRLVHDTGGKAAHWYNQLGR
                     AQVIPSAVLSGVYSPANLEDVQENKGVYLFWQHRLSDLARFAKSVG"
     gene            complement(949..1422)
                     /locus_tag="VFX38_06140"
                     /pseudo
     CDS             complement(949..1422)
                     /locus_tag="VFX38_06140"
                     /inference="COORDINATES: protein motif:HMM:NF021113.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XamI family restriction endonuclease"
     gene            complement(1614..>3045)
                     /locus_tag="VFX38_06145"
     CDS             complement(1614..>3045)
                     /locus_tag="VFX38_06145"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEX5314468.1"
                     /translation="VRSCYVRNAPARPVIDKQFKEERGLVSLCAALIGDLAPLLPAEA
                     KLLSKAHAIQRRAIAEARAQILSGEDPLGAAFCALRSPGERRRHGATYTPAAIVHAMV
                     AWAKDEIANPARIVDPGSGSGRFLVAAARVFREAQLVAVDVDPLATLMLRANAAVHGF
                     ASRLSVHLCDYRSLEMPAVAGVSLFVGNPPYVRHHEIDREWKAWFAATAKRFGVGASK
                     LAGLHIHFFFRTLELAKEGDCGAFITAAEWLDVNYGAALRRLLGNGLGGTAVHIIEPT
                     AQPFPDALTTGAITCFRVGARSAAIRMRSVSSLEDLSRLDRGRAIGWDEIGNAPKWSV
                     FVRGPQLAQDGFIELGELFRVHRGQVTGANELWIDNEAARDVPSRLKLATVTSARELI
                     EAGDELSCANHLRRVIDLPPDLDGLDDGEREAVLQFLAWVKQRGGHRGYSDASPDMVV
                     GRVARASAGFVHVHGAATPSLRSQQG"
BASE COUNT          584 a         1014 c          927 g          520 t
ORIGIN      
        1 ggcggaatac gcccacttcc atgtcgccca ggaacacgac gcggcggtcg ccctccttga
       61 actcggaaac cctgccgacg aactgctccg ccatgacgcc gccgctccct cgttgatctt
      121 ggcgccgcga attctcgcgc cttccgcgtg cgcctgcaat agctcagcca tcctccaggt
      181 agtgaagcgc gagccagagg ctcggccgct ccggatcggt ccattgcacg cggtggcgga
      241 aatgtgccgg aatgtcgagg caatcgccgg ggccgagcgc cacgaccgcg ccgtcggcga
      301 attcgagccg cgccgcgccc gaaaccagca acacccattc gcgccggtcc tgctcgagcc
      361 agtccgtgct ctggcccagc gagacgatac gctcgagccg gaacgcgccg gtctcgacca
      421 gcgcctcgac catttcgccg tcgcggctgg ccggcagggg actgaagaga ttgcgcatcg
      481 aacgactatc cgacggattt agcgaacctg gcgagatcgg acagtcgatg ctgccagaag
      541 aggtaaacgc ccttgttctc ctgcacgtct tcgaggttcg cggggctgta gacgccgctc
      601 agaaccgcgc tcggtatgac ctgcgcgcgg cccaattgat tgtaccaatg cgccgccttg
      661 ccgcccgtgt catggacaag ccgcttatag gaattcacgg tggagttcga gaccttgcat
      721 tcaatggtca tgattcgccc gtctcgcagc cgagccacaa cgtccgcccg cgtgccggct
      781 aaacgcgttt cgccgcagaa ccggccgggc ggcggcgcgg cggtgagaat gggaatatcc
      841 cttgccttca cttcctccat gccggcgctc cccaacacgt ctttgaccgc cttttcctga
      901 gcctccttgg acgtattgcg gcgcgccgac atcgcttgaa gccgcaagcg ccgccgacgc
      961 gatcacggcg cgctcccgct cgtcgcggga tggccggcgg ccttccgcta cccagggaaa
     1021 ccggcgcgga tcgatgacgg tcaagaccgt gtcgcgaatg cgcttcgcgc cctccgcgtc
     1081 aatacgaagc cgggatgcgg tgagcgtggc gtcagcgatg gtctcaagat cgtcctccga
     1141 gatcggcggc gccgtcaaat agcggaatgc cttctgcgcg tcgcggccat cgacgagctc
     1201 ggcaatcagc cgcgcatcga ccggatcggc tgaaatcgtg ggcaacctct cgatcaggcc
     1261 gcggaaaatg tcggcgaacc gcgcgaagaa ctgattatag aggtctagcg gctccagaag
     1321 ccgctcattg cgaaaggcgt cccgcgccag cgctgcgtcg gacttcaatt ccgcctcggt
     1381 ccagtgcttg ggcagccgtt tggctttcgg cgagatagcc atcgatgtct tctaaccgtg
     1441 gcacgaggag tcgctcaagc tccttcggct cgaacttgac cagcccgccc gcgtacgtcc
     1501 tccctcccgc tgtgctcaca tggcgccgca gatacgcgag aatgcgcgtg agcgacccat
     1561 caccgaggtc ctggcgcgga taaaggccat gcgcgatatt gatgtggcag gccctagccc
     1621 tgttgcgaac gaaggctggg ggtcgccgcg ccatgtacgt gcacaaaacc ggcgctggcg
     1681 cgcgcaaccc gaccgaccac catgtccggc gatgcgtcgc tatatcctcg atggccgcca
     1741 cgctgcttga cccaggcaag gaattgaagc acggcttcgc gctcgccgtc gtctaggccg
     1801 tccagatccg gaggcaaatc gatcacccgc cgcaggtggt tggcgcaact cagttcgtcg
     1861 ccggcttcga tcaattcgcg cgcgctcgtg acggttgcaa gctttaggcg gctcggcaca
     1921 tcgcgcgcgg cctcattgtc gatccatagc tcgttggcgc cagtgacttg cccgcgatgc
     1981 acgcggaaca attcgccgag ttcaatgaac ccatcttggg ccagttgggg gccacgcaca
     2041 aacaccgacc acttcggtgc attcccaatc tcgtcccagc caatcgcccg gccccggtcg
     2101 agccgggata agtcctcaag ggagctgacg gagcgcattc ggatcgccgc cgatcgagcg
     2161 ccgacacgaa agcaggtgat agcgccagtc gtgagtgcgt ccggaaatgg ttgagccgtg
     2221 ggctcgataa tatgcaccgc agtcccccct aacccattcc cgagaaggcg acgaagcgcg
     2281 gcaccgtaat tcacatcaag ccattcggcg gcagtgatga acgcgccgca atcaccctcc
     2341 ttcgcgagtt cgagcgttct aaaaaagaaa tgaatgtgca agccggctag cttgctcgcc
     2401 ccgaccccga aacgtttggc agtcgcggcg aaccaagcct tccactcgcg atcaatttcg
     2461 tggtggcgca cataaggcgg gttgccgacg aacagactga cgcctgcgac agcaggcatt
     2521 tccaagctgc gataatcgca aagatgaacc gagaggcgcg atgcaaaccc gtgcacggcg
     2581 gcattggcgc gcaacatgag cgtcgccaat ggatcgacat cgaccgcgac gagttgcgcc
     2641 tcgcggaaca cgcgggccgc ggcgaccaag aaacgacccg atccggaccc cggatcgaca
     2701 atgcgcgcgg ggtttgcaat ttcgtccttg gcccatgcca ccatggcatg aacgatggct
     2761 gccggcgtgt aggtcgcacc atgtcgccgc cgctcccctg ggcttcggag cgcgcaaaac
     2821 gcggccccaa gcgggtcctc gccggagagg atttgcgcgc gcgcctcagc gatcgcgcgc
     2881 cgttggatgg catgagcctt ggacaggagc ttcgcttccg cgggcaacaa cggagcaagg
     2941 tcgccgatca atgcggcgca gagcgagacg agcccccgct cttccttgaa ctgtttatca
     3001 atcacaggac gagcgggggc gtttcgaaca taacaggaac gtaca
//
LOCUS       DASUJG010000285         2796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1306893, whole genome shotgun sequence.
ACCESSION   DASUJG010000285 DASUJG010000000
VERSION     DASUJG010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306893"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1515)
                     /locus_tag="VFX38_06150"
     CDS             complement(19..1515)
                     /locus_tag="VFX38_06150"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006473838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX5314469.1"
                     /translation="MNHNASGGAATARASACVVAGVYQGGRPPPATTALDKASHGRLL
                     GRSHPAFRATSGESLLLGDIPGLKAKHVLLVGLGPRRDFGMAALRKSAATTVRALEDA
                     RIGGALSLLAELPVTGMELETRLQALVQASEHAAYRFEQMKSRATDEAKPRLKRLSWS
                     VSTGDDVAAARRGIAAGAAIAGAEALTRDLSNLPANVCTPRYLAEQARRLGEKYPALQ
                     VNVLDENAIQKLGMGALWGVARASREPPRLIVFEYAGGKRNDRPVVLVGKGITFDSGG
                     ISIKPSAKMDEMKFDMCGAASVFGALAAACELALPINLVGITPAAENLPGGNAVKPGD
                     ILTTLSGQTVEVLNTDAEGRLILCDALCYAERFQPRVVVDMATLTGACVVALGQHASG
                     LMSPDDALAAELLAAGERAGDRAWRLPLWEDYQSELKSNFADFANVGGREGGAIIAGC
                     FLWRFAKKFRWAHLDIAGTAWLSGEKKGATGRPVPLLVEWLMDLASAE"
     gene            1612..2700
                     /gene="lptF"
                     /locus_tag="VFX38_06155"
     CDS             1612..2700
                     /gene="lptF"
                     /locus_tag="VFX38_06155"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEX5314470.1"
                     /translation="MMLGIFGRYVARETAWNWCGVVGVLVLVIVVNRFAVYLGEAASG
                     QIPAGISFVLIGLSVAGLLEIVLPVSLFLAAMLTLGRLYRDGEAVAGFVCGLTPGRLY
                     RPFAVLGVIAALILAWLALYVSPWTAALGHRIEQSARSEASISLLVPGQFKNLPGGGV
                     FYAGGASDDGKKLLDVFAATEEDSQTVMLAGSQGKLISEGENGARHLEVAPGHRYAGI
                     PGQLDWTVTRFRSASLLVRPATPEAGVTDPDRLPTAQLSARDDPVARATLQWRLVQPF
                     TVLVLLLIAVPLAHIRPRQGRFARLVPAILVYLVYFNLLGVVRVWVGEGTIPLLPGLW
                     LVPLVFALGGIGLIYLRFARRLGGRSAA"
BASE COUNT          405 a          897 c          938 g          556 t
ORIGIN      
        1 aaatcgatgc gggtcacgtc attcggccga tgcgaggtcc atcagccact cgaccagaag
       61 cggaacgggt cggccggtgg cgcccttttt ctcgccgctc agccaggcgg taccggcgat
      121 gtcgagatgc gcccagcgga acttcttcgc gaatctccac agaaaacaac ccgcgatgat
      181 ggcgccgccc tcgcgcccgc cgacattggc gaagtccgca aagttgcttt tcaactcgga
      241 ttgataatct tcccagagtg gcagtcgcca cgcgcgatct ccggcgcgct cgcccgcagc
      301 gaggagttcg gcggcgagtg catcgtcggg cgacatcagc cccgatgcgt gctggccgag
      361 cgcgacgacg caggcgcccg tgagcgtcgc catgtccacg accacgcggg gttggaaacg
      421 ctccgcgtag cagagtgcat cacagagaat caggcgaccc tcggcgtcgg tgttgagaac
      481 ctcgacggtc tggcccgaaa gggtcgtgag aatatccccg ggcttgaccg cattgccgcc
      541 gggaagattc tcggccgcgg gcgtgatgcc gacgagattg atcggcagcg cgagttcgca
      601 ggcagcggcg agggcgccga agacgctggc ggcgccgcac atgtcgaact tcatttcgtc
      661 catcttggcc gagggcttga tcgagattcc ccccgagtcg aaggtgatgc ccttgccgac
      721 gaggacgacc gggcgatcgt tgcgtttgcc gccggcgtat tcgaaaacga tcagacgcgg
      781 cggctcgcgg ctcgcgcggg ctactcccca gagcgcgccc atgccgagtt tctggatcgc
      841 attctcgtcg agcacgttca cctggagtgc gggatatttt tcgccgaggc gccgcgcctg
      901 ctcggcgaga tagcgcggcg tgcagacgtt cgccggcaga ttgctcagat cgcgggtgag
      961 cgcctccgct cccgcgatgg ctgcccccgc ggcgatgccg cggcgagcgg cagcgacgtc
     1021 atcgccggtc gaaaccgacc agctcaggcg cttgaggcgc ggctttgcct catccgtggc
     1081 gcgggacttc atctgctcga agcggtaggc cgcatgctcg ctcgcctgca cgagcgcctg
     1141 cagtcgagtt tccagttcca tgccggtcac cggaagttcc gcgagaagac tcagggctcc
     1201 tccaatcctc gcgtcctcga gagctcgcac ggttgtcgcc gcgctcttgc gcagcgccgc
     1261 catgccgaag tcccggcgcg ggcccagtcc caccagcagc acatgtttgg ctttcagccc
     1321 cgggatgtcg ccgaggagca ggctttcgcc gctcgtcgcg cggaaggcag gatgcgaacg
     1381 accgaggagg cggccgtgac ttgctttgtc gagggccgtg gtcgcgggcg gcggccttcc
     1441 gccctgatac actcctgcga ccacacaggc gcttgcgcgc gcggtcgctg cgccgccgga
     1501 tgcgttgtga ttcatgcgct tgctccgtgg agagattttc ttgccggagc cgccccgcgc
     1561 cgacggggcc gcttccggga gcgtttgcga aggtagtcta accgaaagga aatgatgctc
     1621 gggattttcg gtcgctacgt tgctcgcgaa actgcatgga actggtgcgg tgtcgtcggc
     1681 gtgctcgtgc tcgtgatcgt ggtcaatcgc ttcgccgtct atctcggcga ggcggcgagc
     1741 ggccaaattc cggcgggaat ctcctttgtg ctgatcggtc tcagcgtcgc gggcctgctc
     1801 gagatcgtgc tcccggtcag tttgtttctc gcggcgatgc tgacgctcgg gcgcctgtat
     1861 cgcgacggcg aggcagtggc gggcttcgtc tgcggcttga cgccgggaag gctctaccgt
     1921 cccttcgccg tgctcggcgt aatcgccgcc ttgattcttg cctggctcgc gctctatgtc
     1981 tctccctgga cggcggcact gggacatcgc atcgagcaga gcgcccgctc cgaggcatcg
     2041 atcagcctcc tggtcccggg ccagttcaag aaccttccgg gcggcggagt cttttacgcc
     2101 ggcggagcga gcgatgacgg gaagaaactt ctcgatgtct tcgcggccac cgaggaggat
     2161 tcgcagacgg tgatgttggc gggtagccag ggcaagttga tcagcgaggg agagaacggt
     2221 gcgcgccatc tcgaagtcgc gcccggccat cgttatgcgg gaatacccgg gcagctggac
     2281 tggacggtga cgcggttccg cagcgcctcg ctgctggtgc gaccggcgac gcccgaagcc
     2341 ggcgtgaccg atccggatcg cctacccacg gcgcagcttt cggcgcgcga cgatcccgtg
     2401 gcgcgggcga ctctccagtg gcgtctggtc cagccgttta ccgttcttgt tcttcttctg
     2461 atcgccgttc ccctcgcgca tattcgtccg cgccagggtc gctttgcccg cctggtcccg
     2521 gcgattctgg tctacctcgt gtatttcaat ctcctcgggg tcgtgcgggt ctgggtcggc
     2581 gaggggacga ttccgctgtt gccgggcctg tggctggtgc cgttggtttt cgcactcggc
     2641 ggcatcggtt tgatttatct gcgatttgct cgacgtctcg gaggacgtag tgctgcttga
     2701 tcgttatctg agccgcgccg tcttcacggg cgcgctcgtc gctctcgcgg cgttgctggg
     2761 cgtgggcttc tttctgacct ttctaggcca gatcca
//
LOCUS       DASUJG010000286         2043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1429275, whole genome shotgun sequence.
ACCESSION   DASUJG010000286 DASUJG010000000
VERSION     DASUJG010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429275"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VFX38_06160"
     CDS             complement(<1..513)
                     /locus_tag="VFX38_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5314471.1"
                     /translation="MPITEEKAASRRAPARADRPFKGIALILLSTTFLGVSDVTAKYL
                     SASLPSIEIAWLRFLVFAVIMTPAMIPGSPLYAMRISSVGLPLMLMRGAALLGSSLLF
                     ITGLRYLPIAEASATGFVAPLFVTALSIVFLSEKVGVRRWLATATGLFGVLIILRPGT
                     SAFHAAAFFPI"
     gene            complement(883..1722)
                     /locus_tag="VFX38_06165"
     CDS             complement(883..1722)
                     /locus_tag="VFX38_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5314472.1"
                     /translation="MKKKQFGKGGPHVSVIGQGTWYLDRGDRESAIAALRQGVELGMT
                     HIDTAEMYGDAELVIAAAIADKRDDVFLVSKVLPSNASRRGTITACERSLKRLKTDHL
                     DCYLLHWRGSYPLSETVAAFEELKRAGKIRSWGVSNFDADDLDELLEVSGESKIACNQ
                     VLYHLKERAVEHAVIPWCARHGVAVVAYSPFGHSDFPSLASSGGKVLQKIAAHHKATP
                     RQVALSFLARETTVFPIPKASSAEHAAENAAAGELVLSEAEISALDAAFPRGAKPRSL
                     PML"
     gene            complement(1864..>2043)
                     /locus_tag="VFX38_06170"
     CDS             complement(1864..>2043)
                     /locus_tag="VFX38_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX5314473.1"
                     /translation="HASAGHVFVFAPNGECIARIKSCTGGACTNVAIGGASRDRLYIT
                     ESMSGSVLVADISVL"
BASE COUNT          414 a          634 c          634 g          361 t
ORIGIN      
        1 gatcgggaaa aaagccgcgg cgtgaaacgc gctcgtgccc ggccgcagga tgatcagcac
       61 gccaaagagc ccggtcgcgg tcgcgagcca gcgccgcaca ccgacctttt cagaaagaaa
      121 gacgatcgac agcgcggtga cgaacaaggg cgcaacgaag cccgttgcgg acgcttccgc
      181 gatcggcaga taacgtagtc ccgtgatgaa caacagcgag gagccgagca gcgcggcgcc
      241 acgcatcagc atcaagggca gcccgacgct ggagatccgc atcgcatata atggcgatcc
      301 gggaatcatc gccggcgtca tgatcaccgc gaacaccaga aagcgcagcc acgcaatttc
      361 gatcgagggc agactcgccg aaaggtattt tgcggtgacg tctgagacgc cgaggaacgt
      421 ggtcgagagc aggatcagcg cgatgccctt gaacggccga tcggcgcgcg ccggtgcgcg
      481 gcgcgacgca gccttctctt cagtgatcgg cagcgacggg ctgccggtcg cttcggccgc
      541 ggcgggcttc ttttcaggca gagacaaacg tgcgctctcg agcctaacgg ctgcggcgga
      601 tggtggagtc acgagcaaac tcgcgcagaa attttcgaat caggcgaggt cataaagaac
      661 gacaaacgag cccgcacgac aacacccgtt agcgggacgc cgatatgcag catacgggac
      721 gcgctgaaac aatttgttaa gggcggatcg ctgtttcttg tcattgctgg gcttgaccag
      781 gctcgacctg gcaatccgtc tcacttgagc aaaaagcttt ttctttttga tggatgcgcg
      841 gatcaagccc ccgcatgacg agtggagaag gcacgcgaca attcacagca tcggcaaact
      901 tcgcggcttg gccccgcgcg gaaacgccgc atccagcgcc gatatttccg cctcgctcag
      961 cacgagttcg cccgctgccg cattttccgc cgcgtgttcg gcactggagg ctttgggaat
     1021 cgggaacacc gtagtttcac gggcaagaaa gctgagcgcg acctgacgcg gtgtcgcctt
     1081 gtgatgcgcg gcgatcttct gcagtacctt gccgccgctg ctcgcaagcg acgggaagtc
     1141 gctgtgcccg aacggcgaat aggcgacgac ggcgacgcca tgccttgcgc accatgggat
     1201 caccgcgtgc tcgaccgcgc gctccttcag atgatagagc acctggttgc aggcgatctt
     1261 gctttcgccc gacacttcca gaagttcatc gagatcgtcg gcgtcgaaat tgctgacgcc
     1321 ccaggagcgg attttccccg cacgtttgag ctcctcgaac gccgcgaccg tctccgacag
     1381 tggataggag ccgcgccagt gcagcagata gcagtcgagg tggtcggtct tcagccgctt
     1441 cagcgagcgc tcgcaggcgg tgatggtgcc gcgccgcgaa gcgttgctcg gcagcacttt
     1501 cgagaccagg aaaacgtcgt cgcgcttgtc cgcgatcgcg gcggcgatga cgagttcggc
     1561 atcgccatac atctcggcgg tgtcgatgtg cgtcatgccg agctcgacac cttgacgaag
     1621 cgctgcgatt gcactctcgc ggtcgccgcg atcgagatac caggtgccct gcccgatcac
     1681 ggagacgtgc gggccgccct tgccgaattg ttttttcttc acaaaagcct cttacgactg
     1741 atgccgtcat tgcgaggagc caacgggtcc ggccttttgg ccggcccgat gacaggctcc
     1801 gcgacgaagc aatccactct tgctcgccgg tgacaatgga ttgcttcgct tcgctcgcaa
     1861 tgactagagt acactgatat cggcgaccag cacgctgccg ctcatggact ccgtgatata
     1921 gagccggtcc cgactggcgc caccgatcgc cacattcgtg caggcgccgc ccgtgcagga
     1981 cttgatgcgc gcgatgcact cgccgttcgg cgcgaacaca aagacgtggc cggccgacgc
     2041 atg
//
LOCUS       DASUJG010000287         4362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1429725, whole genome shotgun sequence.
ACCESSION   DASUJG010000287 DASUJG010000000
VERSION     DASUJG010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429725"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="VFX38_06175"
     CDS             complement(<1..887)
                     /locus_tag="VFX38_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HEX5314474.1"
                     /translation="MSSMWTDTDTCYRALLARDSRFDGQFFVGVSSTRIYCRPVCTVR
                     APMRKNCRFYPSAAAAEAAGHRPCLRCRPELAPGHASVDASRRLARAAAGLIDDGALS
                     DKGLGSLAGRLGTTDRHLRRVFAEEFGVSPVAYAQTQRLLLAKRLLTDTRLPVTEIAM
                     AAGFGSLRRFNALFRERYRMQPRELRKTPEAAARGELVFELAYRPPYAWEPQLAFLGL
                     RCTQGVEAVIGKRYLRTVAVARKGATHCGWIAVAHRARRATLQITLFGSLAAVVPEVL
                     GRVKHLFDLACRPDEIAAHL"
     gene            complement(968..1360)
                     /locus_tag="VFX38_06180"
     CDS             complement(968..1360)
                     /locus_tag="VFX38_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009569596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 family protein"
                     /protein_id="HEX5314475.1"
                     /translation="MFTLFWIVLAAHVAIIAFNVIGLIVIPLGAWRRWAWVREFWWRL
                     AHLVILAAVAAQALAGRACILTLWQSEMAGGGKPAPLIAGWVERVIYLPLPLWVFTVI
                     YVVVWVYVLALWWRVPPRMPWKRTGRTA"
     gene            complement(1479..3305)
                     /gene="recQ"
                     /locus_tag="VFX38_06185"
     CDS             complement(1479..3305)
                     /gene="recQ"
                     /locus_tag="VFX38_06185"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004999791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEX5314476.1"
                     /translation="MTAESILKATFGYAGFRGRQREIIETVAGGGDALVLMPTGGGKS
                     LCYQIPALMRPGIGLVVSPLISLMANQVAALRQVGVAAASLNSSLDFSEQHAVERQMR
                     AGDLDLVYIAPERLLQPRSLELLADCRIALIAIDEAHCVSQWGHDFRPEYLQLAALAE
                     QFPGVPRIALTATADLPTRKEILERLRMPEAQMFVHGFDRPNIRYRVAERREGSGQLL
                     RFIGDEHRRETGIVYCLSRAKTERTAESLAARGVAAFPYHAGLDHALRERHQRRFESD
                     DGIVIVATVAFGMGIDKPDVRFVAHMDMPASIESYYQETGRAGRDGLPSDAWMLYGLN
                     DVVQRRRMIEQSEASEDRKWIEREKLEAMLSYCELASCRRVNLLRYFGEDLHDDCGNC
                     DNCLFPPETWDASEAARKLLSAVYRTGQRFGAQYLVGHLRGEADERAERLGHTRLSTF
                     GIGTELEPGQWRSVIRQLVARRYLESDPDGFGGMRFGAGAEALLHGKQNIRLRYDGKP
                     KRRRRRTERTAIEATSPDAVLWEALRAKRKQLAEAQNVPVYMIFSNSTLREMLAHRPA
                     SPADFLTLNGVGEKKLATYGAEFLQIIAEHENAARDWSPGPL"
     gene            3631..>4362
                     /locus_tag="VFX38_06190"
     CDS             3631..>4362
                     /locus_tag="VFX38_06190"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5314477.1"
                     /translation="MSAARPLRFPAWLGPLLRDPRFAATVIVVLALGIGINVATLGLL
                     YRYYVSPLPFPEGGRMLNVYFTSNQPTPPLMSIPTWQRLKKGAPALADSGLYRDQGYN
                     VMSGGRQSRINGIEATASVFSTLGVQPAVGRVFGPASNKPGARPVVVLSYRLWQTLFG
                     GKSSAVGQTLELNGKLFTVIGVMPKDFNFPTAQAALWTPQIITAFDQDADMLTAWSDS
                     MVARLAPGATRTQLLAQADRVLKNEI"
BASE COUNT          793 a         1457 c         1368 g          744 t
ORIGIN      
        1 agatgggccg cgatttcgtc gggacggcag gcaagatcga agagatgctt gacgcggccg
       61 agcacttcgg gcacgacagc cgcgagcgaa ccgaaaagcg tgatttgcag cgtggcgcgg
      121 cgcgcccgat gcgcgaccgc gatccagccg caatgcgttg cccccttgcg cgcaacggcg
      181 accgtgcgca ggtaccgctt tccgatcacg gcctcgacgc cctgcgtgca gcgcaggccg
      241 agaaacgcca actgcggttc ccaggcgtac ggaggccggt aggcgagctc gaagacgagt
      301 tcgccgcgcg ccgccgcctc gggcgtcttg cgcaattcgc gcggctgcat gcgataacgt
      361 tcgcgaaaca gcgcattgaa tcgccgcagg ctgccgaagc ccgcggccat cgcgatctcc
      421 gtcaccggca ggcgcgtgtc ggtcagcagg cgtttggcga ggagcaatcg ctgcgtttgt
      481 gcgtaggcga ccggagaaac cccgaattcc tcggcaaaga cgcggcgcag atgacgatcg
      541 gtcgtgccga ggcgccctgc cagcgagccg agtcccttat cgctcagcgc gccgtcgtct
      601 atcaaccctg ccgcggcgcg ggcgagacgc cggctcgcat ccacgctcgc atgaccgggc
      661 gcgagttccg gccggcagcg caggcatggg cggtggccgg ccgcctccgc cgccgcggcg
      721 ctcggataaa agcggcaatt cttgcgcatg ggcgcgcgca cggtgcagac gggacggcaa
      781 tagatgcggg tcgaggaaac gcccacgaaa aactggccgt cgaagcggct gtcgcgggca
      841 aggagcgccc gatagcaggt gtcggtatcg gtccacatgg aggacatgat agtccgatgc
      901 gaacggcaat actagccgtt ttcggacatc agattggcgc ccgggagtgg gccgggtttc
      961 ccgtccgcta tgccgtccgt ccggttctct tccacggcat ccgcggcggc acgcgccacc
     1021 agagcgcgag cacataaacc cagacgacga catagatcac ggtgaaaacc cagagcggaa
     1081 gcggcagata gatcacccgc tcgacccagc ccgcgatgag cggggccggc tttccgccgc
     1141 ccgccatttc gctttgccat aacgtgagaa tgcaggcccg gcccgcgagc gcctgcgccg
     1201 cgacggccgc cagaatcaca agatgcgcaa gccgccacca aaattcgcgc acccatgccc
     1261 agcgccgcca tgctcccagc gggatcacga tcagtccgat tacattgaac gcgatgatcg
     1321 cgacatgcgc ggcgagcaca atccagaaaa gtgtgaacat gcaggctttc cctgtcgtca
     1381 caacgcccgc cctgcgccaa ttgtaggccg ggataagcgt agcgttcccg gcatgccacc
     1441 cgccgaatgc cggcaacggc catcggcctt atgccggcct acaaaggccc cggggaccaa
     1501 tcgcgggcgg cattttcatg ctctgcgatg atctgtagaa actcggcgcc ataagtcgcg
     1561 agtttcttct cgcccacacc attgagagtg aggaaatctg cagggcttgc cgggcggtgc
     1621 gcgagcattt cgcgcagggt ggagttggag aaaatcatgt agacgggaac gttctgcgct
     1681 tccgcgagct gtttacgttt cgcgcgcagc gcttcccaca ggacggcgtc gggcgacgtc
     1741 gcttcgatcg cggttcgttc cgttctcctg cgccgccgtt tcggcttgcc atcatagcgc
     1801 agccggatgt tttgcttccc gtgcagcaag gcttcggcgc cggcgccgaa gcgcatcccg
     1861 ccaaagccat ccggatcgct ttcgagataa cggcgggcaa cgagctggcg gatgaccgaa
     1921 cgccactgcc cgggctcgag ttccgtaccg atgccgaagg tggacagcct cgtgtggccc
     1981 agccgctccg cgcgttcgtc cgcctcgccg cgcaagtgac cgacaaggta ctgcgcgccg
     2041 aaacgctggc cggtgcgata caccgcggaa agcagcttgc gcgccgcctc gctcgcgtcc
     2101 caggtctcgg gcggaaagag acagttgtcg cagttgccgc aatcgtcgtg cagatcttca
     2161 ccaaagtagc ggagcagatt gacgcggcgg cagcttgcga gttcgcagta ggaaagcatc
     2221 gcctcgagct tctcgcgctc gatccatttg cgatcttcgc tcgcctccga ttgttcgatc
     2281 atgcgccggc gctgcaccac atcgttcaat ccgtacagca tccatgcgtc gctcggcagg
     2341 ccgtcgcgac cggcacggcc ggtttcctgg taatagcttt cgatgctcgc cggcatgtcc
     2401 atgtgcgcga cgaaacgcac gtcgggtttg tctatgccca tgccgaaagc cacggtcgcg
     2461 acgatcacga tgccgtcgtc gctctcgaag cggcgttgat gacgttcgcg cagggcatga
     2521 tcgagcccgg catgataggg aaaggcggca acgcctcgcg ccgcgagact ctccgccgta
     2581 cgttcggtct tcgcgcgcga gaggcaatac acgatgccgg tttcgcgccg atgttcgtcg
     2641 ccgatgaatc gcaacagttg cccgcttccc tcgcgccgtt cggccacccg gtagcggatg
     2701 ttcggccgat cgaagccatg aacgaacatt tgcgcctcgg gcatccgcag gcgttccaga
     2761 atttccttcc gggtcggcaa atcggccgtg gccgtgagcg cgattctcgg cacgccggga
     2821 aactgctcgg ccagcgcggc gagttgcaaa tattcgggcc ggaaatcgtg gccccactgc
     2881 gatacgcaat gcgcctcgtc gatcgcgatc agcgcgatcc ggcaatccgc tagcaattcc
     2941 agggagcgcg gctgcaggag ccgttccggc gcgatgtaaa cgagatccag gtcgccggcg
     3001 cgcatctgcc gctccacggc atgctgctcg gagaaatcaa ggctcgaatt cagcgatgcc
     3061 gccgcaacgc ccacctgccg cagcgccgca acctgattcg ccatcaagga aatcagcggc
     3121 gaaaccacga ggccgatgcc ggggcgcatg agcgccggaa tctggtagca gagcgacttg
     3181 ccgcccccgg tcggcatcag taccagcgcg tcgccgccgc cggcgaccgt ttcgatgatt
     3241 tcccgctggc gtccgcgaaa cccggcgtag ccgaacgtcg ctttcagaat gctttccgcc
     3301 gtcatggcgt gcccccactc tttgattatg gccgcgactc agggtaccgc aaacacctcg
     3361 ctgccggccg gcacgaaaaa gtccggattt cgcccggcaa gcccgatccg gcccacgcgc
     3421 aggccggata cccgcaatcg aagccgcgcg acagcggttc caatcccggt aatgggatcg
     3481 gccgtggagg cgatcccaac actgcgacgc cggagccccg ccgttccaag ccgatttttc
     3541 tttttttccg tgcagtcaaa aacttatgga aacgcgagcg atttggcacg gttcttgctg
     3601 gcctatgggt aacgattttc gagatgatcc atgtccgccg cacgtccgct tcgattcccc
     3661 gcatggctcg gcccgctcct gcgcgacccg cgcttcgcgg cgacggtgat cgtggtgctc
     3721 gcgctcggca tcggcatcaa cgtcgcaacg cttgggctgc tctatcgcta ctacgtctcc
     3781 ccgcttccgt ttcccgaagg cggccggatg ctgaatgtgt acttcacgtc caatcaacca
     3841 acgccgccgc tgatgagcat accgacctgg cagcgattga agaagggcgc accggcgctc
     3901 gccgattcgg ggctctaccg cgaccagggg tacaacgtca tgagcggcgg ccggcagagc
     3961 cgcatcaacg gcatcgaggc gacggcttcg gtcttctcga cgctcggcgt gcagcccgca
     4021 gtcgggcgag tcttcggccc cgcgagcaac aagccgggcg cgcggcccgt cgtcgtcctg
     4081 agctaccggt tgtggcagac gcttttcggc ggcaagtctt cggccgtcgg ccagaccctc
     4141 gaactgaacg gaaaactctt cacggtgatc ggcgtgatgc cgaaggactt caattttccg
     4201 accgcccagg ccgcgttgtg gacgccgcag atcatcaccg ccttcgatca ggatgccgac
     4261 atgctcacgg cctggagcga cagcatggtc gctcggcttg cgccgggcgc gacccgcacg
     4321 caacttcttg cccaggcgga tcgcgtgctc aagaacgaga tt
//
LOCUS       DASUJG010000288         3258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_817400, whole genome shotgun sequence.
ACCESSION   DASUJG010000288 DASUJG010000000
VERSION     DASUJG010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817400"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            249..1574
                     /locus_tag="VFX38_06195"
     CDS             249..1574
                     /locus_tag="VFX38_06195"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5314478.1"
                     /translation="MTFNPIAYDPAAHRPAPRRYAWSVFAILFALMVFDYIDRQVVVS
                     MFPHLKAQWDLSDSELGGLVSIVSVTVALGAVPLSLLADRWSRVKSIFIMVLIWSLAT
                     ISCAFAGSYTHLLGARSVVGLGEAAYGTVGAALLASLFPLRMRSTVLGAFLAAAMIGS
                     VFGVMLGGFIADRWGWQAGFGAVGVPGLLLCFVFLAVVRDYRTVALPRSDRPNAAPKT
                     AARAVVTQLLRPRTALVTCIGAGLNLLVVSTTYAWLPSYFNRYYGLAPDQAGGKTALV
                     VLVGGIGMVLWSVVADRLTPRLPRARLLIPAVTAVLTAVLMIAAFAAFPPGAAQFGLI
                     VAGGLLMAGSVGTTDAVIIDVVHPSVRATAASILSLTRNLFGLAGGPLLTGALSDAYG
                     LPFAMSVVPVFCFFAAALYLIAARTYEADLKFTAATAPEPAGRLEPQVA"
     gene            1577..1987
                     /locus_tag="VFX38_06200"
     CDS             1577..1987
                     /locus_tag="VFX38_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX5314479.1"
                     /translation="MPSADVRLVDTAPSRKARQPRARKQAGGNWNPDWEPFAKLDPAW
                     TEKIVALAIAPAVAGALDAKTIELIGIALDASCTHMYAPGVRRHIRRALKAGASREEI
                     VAVLQLTTLQGLHSMCLGAPILLEELENRAQGRD"
     gene            2024..3085
                     /locus_tag="VFX38_06205"
     CDS             2024..3085
                     /locus_tag="VFX38_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX5314480.1"
                     /translation="MNFIDGSLFPEHQEKLVITCAPYGPEWMPSDFPEDIPVTMEAHI
                     QKAVDCYNAGATVLHVHVRELDGKGSKRLSKFNELMAGIRARVPELILQVGGSISFAP
                     EGEGEVAKWLSDDTRHMLAELVPTPDQVTIAINSNQMNVVEQMCDADLTGTSLAEPGN
                     YRAYREMTIPAGPEWVEEHIRRLSANGIQTHFQLANITQLETVERMMRRGTCNVPLIL
                     TWVAIGGGFDAPNIYNLANFVRAVPDGAVLTLETSMLNVLPINMMAIAMGLHVRCGIE
                     DNLWTQDRKAKMSSVRQIEQLVRISGEFGRPVANAREARAIYRIGEFYQDADETLAKN
                     GFAPNRKPGERGVPLRAAA"
     gene            3114..>3258
                     /locus_tag="VFX38_06210"
     CDS             3114..>3258
                     /locus_tag="VFX38_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEX5314481.1"
                     /translation="MAQAIRFDRPGGPEVLRWEEVAVGDPGPGQARIRHVAVGLNFAD
                     TYFR"
BASE COUNT          546 a         1090 c         1041 g          581 t
ORIGIN      
        1 cggactcctg cagggagcgc tgcggcaggc cgaactcgcg cagcatgcga ccggggtcga
       61 gacccgcctt gcgggcgacc tcggcgtagt tggtgagact cgcgctgcgg acctggatgg
      121 gcacgatcgc gttccgggga gcctgtcccc aaatggcaag tctatgtcac tcgcgagcaa
      181 ctctcaacgg gtcaacgtcg gcagaattcg gctgcggaca aacgaaatca ccgagaccac
      241 tcctgaccat gacgttcaac ccgatcgcat acgacccggc ggcccatcgg cctgcgccgc
      301 gacgctacgc ctggtccgtt ttcgcgatcc tcttcgcact gatggttttc gattacatcg
      361 accgccaggt cgtggtgtcg atgtttccgc acctgaaagc gcagtgggac ctctcggaca
      421 gcgaactcgg cggtctggtc tcgattgtgt ccgtcaccgt cgccctcggt gcggttccgc
      481 tgtcgctcct cgccgatcgc tggagccggg tgaagagcat cttcatcatg gtgctgatct
      541 ggagcctggc gacgatctcg tgtgcgttcg ccggcagcta cacgcacctg ctcggcgcac
      601 gcagcgtcgt ggggctgggg gaagcggcct acgggaccgt cggcgcggcg ctgctcgcga
      661 gccttttccc gctgcgcatg cgcagtaccg tgctcggcgc gtttctcgcc gccgcaatga
      721 tcgggtcggt tttcggcgtg atgctcggcg gattcatcgc tgaccgctgg ggctggcagg
      781 ccgggttcgg cgccgtcggc gtaccgggcc tgctcctttg cttcgtcttt cttgcggtcg
      841 tccgcgacta ccggaccgtc gcactcccgc gcagcgaccg acccaatgcc gcgccgaaga
      901 ccgcggcgcg tgccgtggtt acgcaactac tccggccgcg cacggcactc gtcacctgca
      961 tcggggcggg actcaacctc ctcgtcgtgt ctacgaccta cgcctggcta ccaagctatt
     1021 tcaaccgcta ttacggtctc gcgcccgacc aggcaggcgg caagacggcg ctggtcgtac
     1081 tggtcggcgg catcggcatg gtgctctgga gcgtggtggc tgatcgcttg actccacgcc
     1141 tgccgcgcgc acggcttctg attcctgcag tgacggccgt cctgaccgcc gtgctcatga
     1201 tcgccgcgtt cgcggccttc ccgcccgggg ctgcgcagtt cggcctgatc gtcgcgggtg
     1261 ggctgttgat ggccggcagc gtgggaacca ccgatgcggt catcatcgac gtcgttcatc
     1321 cgagtgtgcg cgctaccgcg gcatcgattc tctcgctgac gcgcaacctc ttcgggctcg
     1381 ccggcggccc tctgctcacg ggcgcgctct ccgacgccta cggcctgccc ttcgcaatgt
     1441 ccgtcgtgcc ggtgttctgt ttcttcgcgg ctgcgctcta cctgatcgcg gcccggacct
     1501 acgaggccga tctgaaattc accgcggcga ccgcgcctga gccggccggt cgactcgaac
     1561 cacaggtagc ctgacgatgc cgagcgccga cgtacgtttg gtcgacaccg ccccttcgcg
     1621 aaaggcgaga cagccgcgcg cgcgaaagca ggccgggggc aactggaatc ccgactggga
     1681 acccttcgcc aagctcgatc cggcgtggac cgagaagatc gtcgcgttgg cgattgctcc
     1741 cgcggtggcc ggcgcactcg atgccaagac gatcgagctc atcggcatcg cgctcgacgc
     1801 atcgtgtacg cacatgtacg cgccgggcgt tcgccgacac atccggcgcg cgctcaaagc
     1861 cggggcgtcc agagaggaaa tcgttgcggt gctgcagctt acgacgctgc agggcctgca
     1921 ctccatgtgc ctcggggctc cgatcctgct cgaggaactg gaaaaccgcg cgcagggcag
     1981 agactgactt gtcgtcaaag caaacccgaa aaaggaatcc accatgaact tcattgacgg
     2041 atcgttgttt cccgagcacc aggaaaagct tgttatcacc tgcgcaccgt acgggccgga
     2101 gtggatgcct tccgacttcc ccgaggacat cccggtaacg atggaggcgc atatccagaa
     2161 ggcggtcgac tgctacaacg ccggcgcaac ggtgctgcac gtccacgtgc gcgaactcga
     2221 cgggaagggt tcgaagcgcc tgtcgaagtt caatgagctg atggccggca tccgcgcccg
     2281 cgtgcccgag ctcattctgc aggtcggcgg ctcgatctcc ttcgcgcctg agggcgaagg
     2341 cgaggtcgcc aagtggctct cggacgacac acgccacatg ctggccgagc tcgtccctac
     2401 gccagaccag gtgacgatcg ccatcaacag caatcagatg aacgtggtcg agcagatgtg
     2461 cgacgcggac ctcaccggga cgtcgctcgc cgaacccggc aactatcgcg cctaccgcga
     2521 gatgacgatc ccggcgggtc ctgaatgggt cgaggagcac atccgcaggc tttccgccaa
     2581 cgggattcag acgcacttcc agctcgccaa catcacccaa ctcgagaccg tcgaacgcat
     2641 gatgcgccgc ggcacgtgca acgttccgct gattctcacc tgggtcgcga tcggcggcgg
     2701 cttcgatgcg ccgaacatct acaacctcgc caacttcgtg cgtgcggtgc cggacggggc
     2761 ggtcctcaca ctcgaaacct ccatgctcaa tgtgctgccg atcaacatga tggcgatcgc
     2821 gatggggctg catgtgcgct gcggcatcga ggacaacctc tggacgcagg accgcaaggc
     2881 gaagatgtcg agcgtcaggc agatcgagca gctcgtccgt atctcgggcg agttcggtcg
     2941 cccggtcgcc aatgccaggg aagctcgcgc aatctaccgg atcggcgagt tttatcagga
     3001 cgcggacgaa acgctggcga agaatggctt cgcccccaac cgcaaaccgg gtgagcgggg
     3061 cgtgccgttg cgcgcggccg cttgaccagg ctcccgacac agggatgatg aaaatggctc
     3121 aggcaattcg attcgaccgg ccgggtggac cggaggtgct gcgctgggaa gaggtcgccg
     3181 tgggcgaccc cggccccggc caggcgcgga tccgtcacgt tgcggtcggc ctcaacttcg
     3241 ccgataccta cttccgca
//
LOCUS       DASUJG010000289         6065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_451848, whole genome shotgun sequence.
ACCESSION   DASUJG010000289 DASUJG010000000
VERSION     DASUJG010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6065
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451848"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="VFX38_06215"
     CDS             <1..749
                     /locus_tag="VFX38_06215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX5314482.1"
                     /translation="VAREHVMDSFFGFARACGVSERRLEWNIPVPPAAAERAREVIPA
                     GMPALLISPCANARFRNWRNWPYERYGPVADYAAKELGWRVIVTGGPSEAEREAARTI
                     VAASRCEIVNLQGNTSLKELLALIARARVVITPDSGPAHMATAVGTPVIGLYATTNPE
                     RAGPYLSQRWRIDRYPDALRRFVGKSVEEVPWGTRVRDPRAMALISSEEVIGMLANLA
                     ASSVQQIAEDQRHAEGQHHDERGPVQEPGE"
     gene            complement(660..1646)
                     /locus_tag="VFX38_06220"
     CDS             complement(660..1646)
                     /locus_tag="VFX38_06220"
                     /inference="COORDINATES: protein motif:HMM:NF023828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HEX5314483.1"
                     /translation="MSPTGAETPLRGEEDAPAEDQGAAVAEASHRCRNCGAALSGHYC
                     ATCGQKNHDYAEPFRDVVEEFASEHFNFDAKFFRTVLPLLFRPGFLTVEYFAGRRARY
                     LGPVRLYIFSSILLFFVAWTFAPLPSLQDRGAAGDKAATAAAAKIAKNPHLSAQQKKE
                     LTKAVGGKLPKTVSGKLFGRKFNVNTVELAGAFKQTFEQWLPRLLFVFVPLVALLLKL
                     LYFRPKRYYMEHLVFMLHTHAFIFVAMLVMVSLQILSARASWLATPSGYIDFFIWCYM
                     VVYVFLAMRAYYRQSFLRTLFKFLLSGLLYWTALVVVLSFGVSLILGDLLHA"
     gene            1989..3440
                     /locus_tag="VFX38_06225"
     CDS             1989..3440
                     /locus_tag="VFX38_06225"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX5314484.1"
                     /translation="MRGSVLYQNAEVEPFVAVNPANPAHVVGVWQQDRWSDGGAHGLM
                     AAASFDGGKTWAPRALVMSRCAGGNAGNGADYARASDPWVTISPDGAAYAISISFSGV
                     TLQPNSTSSVLVSRSADGGQTWSDPLTLILDGPDAFNDKESIAADPNNENYVYAVWDR
                     LTTANTGAAMLARTADGGASWQPAQVLYDPGANNQTLNNQLAVLPDGSVIAFFTEIDN
                     AFQGTPNAHMAVVRSTDHGATWSAPVKVADIHAVGARDPETGQDIRDGAFLGQIVAGP
                     DGKLFVVWQDARFSGGQRDGVLISQSGDGGLSWSTPVEINGDPAVQAFEPAVAVRGNG
                     TIGVSYYDFRDDTADRATLPTDYWLTQSADGASWKERRIAGPFDFDLAPDAEGLFTGD
                     YQGIVAPDDIGFLPFFVQTTDEGTANRTDVFAFPPRGGSESLGSAVAVRSVMREPLPM
                     SPEFRRRVQSRLLDLLREEIPGRGKPEAPPVQP"
     gene            complement(3563..4768)
                     /gene="tyrS"
                     /locus_tag="VFX38_06230"
     CDS             complement(3563..4768)
                     /gene="tyrS"
                     /locus_tag="VFX38_06230"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX5314485.1"
                     /translation="MAANCEHPDLAELVRGAADVIKREELDERLKAGRSLVVKAGFDP
                     TAPDLHLGHTVILNKLRQFQERGHAIVFVIGDFTGMIGDPSGRSATRPALGPADIAAN
                     AETYRAQVGSILELAKTRIAFNSEWLGKLSAADLVRLAGHMTVARMLERDDFEQRYRA
                     ESPISLHEFLYPLAQGYDSVALRADIELGGTDQRFNLLVGRQLQTAHGQTPQVVMTMP
                     ILEGLDGVQKMSKSLGNAIGLTDPPDEMFGKLMSISDEIMWRYFGLLSARSLAELDVL
                     KLEVADGGNPRDVKFLLAEEMVTRFHGADAARKAREDFVARFARGETPQDMPEVRIAG
                     EHPLAKLLKEAGLVPSTSEANRMLQQGAVRVDGERVENRELRLAAGKTYVIQVGKRRF
                     ARVAIAEAT"
     gene            complement(4843..>6065)
                     /locus_tag="VFX38_06235"
     CDS             complement(4843..>6065)
                     /locus_tag="VFX38_06235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5314486.1"
                     /translation="AHAMSAHGIVFGAPEIRLDDLLRFKQTAVDRLTGGLAELAKKRK
                     VRVVIGNARFVSPQLLEVETDRKKQTIAFAQAIVAAGSEAMRLPEQPDDPRIMDSTGA
                     LELAEVPKKLLVVGGGIVGLEMAQVYAALGAAVTLVELTGGLMPGTDRDLVKPLAKRL
                     EELFDAIHLETRVAGIEPQEDGVHVRCIGPRGEWSAGFERVLVAIGRRPNGHAIGAEA
                     AGITVDEHGFIPTDEQMRSNVKHIFAIGDIAGPPLLAHKASHEGKVAAEVACGGKSAF
                     DVRAIPAVAYTHPEVAWAGLTETEAQKTGREIERGVFPWQANGRALTLGVDFGFTKLL
                     FDRASGRIVGAGIVGPSAGELISELVLAIEMGCDAEDIALSIHPHPTLAESVGMAAEI
                     AAGTITDLYLPRKR"
BASE COUNT         1048 a         2031 c         1957 g         1029 t
ORIGIN      
        1 atgtagcgcg cgaacacgtg atggacagct tcttcggttt cgcgcgcgcc tgcggcgtga
       61 gcgagcggcg tctcgaatgg aacattcccg taccgcccgc ggcggcggaa cgcgcccgcg
      121 aagtcattcc cgccgggatg ccggcgttgc tcatcagtcc ctgcgcgaat gcacgctttc
      181 gcaactggcg caactggccg tacgagcgct acggtcccgt cgccgattac gccgcgaaag
      241 aactcggctg gcgcgtgatc gtcaccggag ggccgagcga ggcggaacgc gaggcggctc
      301 gcacgatcgt cgccgcgagt cgctgcgaga tcgtcaattt gcagggaaac acatccctca
      361 aggaactcct cgccctgatc gcccgagcgc gcgtcgtcat cactcccgat tcgggacccg
      421 cgcacatggc gacggccgtc ggcacgcccg tgatcgggct gtacgcgacg accaaccccg
      481 agcgcgcggg gccgtatctc agccagcgct ggcgcatcga tcgctacccg gatgcgctgc
      541 ggcggttcgt cggcaagagc gtcgaagaag tcccctgggg cacccgggtg cgagacccgc
      601 gcgcgatggc cctcatctcc tccgaggaag tgatcggcat gctcgcgaac ctcgcggcat
      661 caagcgtgca gcagatcgcc gaggatcagc gacacgccga aggacagcac cacgacgagc
      721 gcggtccagt acaggagccc ggagagtaga aacttgaaca gggtccggag aaacgattga
      781 cggtagtagg cgcgcatcgc gagaaacacg tacaccacca tgtagcacca tatgaagaag
      841 tcgatgtagc ccgaaggggt ggcgagccag ctcgcccgcg cggaaaggat ctgcagcgac
      901 accatcacga gcatcgccac gaagatgaag gcgtgggtat gcagcatgaa aaccagatgt
      961 tccatgtagt agcgtttcgg acgaaagtag aggagtttca ggagcagcgc caccagcggg
     1021 acgaagacaa aaagaagccg cgggagccat tgctcgaagg tttgcttgaa ggcccccgca
     1081 agctccaccg tattgacgtt gaatttccgc ccgaacaatt tccccgacac cgtcttgggg
     1141 agcttgcccc cgaccgcctt cgtcaactct ttcttctgct gggcggacaa atgcggattc
     1201 ttcgcgatct tcgccgccgc ggcggtcgcg gccttgtcac ccgccgcgcc gcggtcctgc
     1261 aggctcggca gcggtgcaaa ggtccaggcg acgaagaaca ggaggatgct gctgaagatg
     1321 tacagccgca ccggcccgag atagcgcgcc cgccgtccgg caaagtattc caccgtgaga
     1381 aaaccggggc gaaagagcag cggcagaacc gtgcgaaaga atttggcgtc gaagttgaaa
     1441 tgttcgctcg cgaattcttc caccacatcg cgaaacggtt ccgcgtaatc gtgattcttc
     1501 tgcccgcagg tcgcgcagta atgcccgctc agggccgccc cgcaattgcg gcagcgatgc
     1561 gacgcctcgg cgacggcggc gccttgatcc tccgcaggtg cgtcctcttc gccccgcagc
     1621 ggcgtctccg ctcccgttgg cgacaccgaa accatacgaa gcctcaccga cacgcgagca
     1681 gcacagccta gcaccgccgc gccctcgccg cgacaaggtg cttttccgcc tgcccgtgaa
     1741 ggtgcaaaat agggaatgat tgatcgtcgc cgaatgagta tgggctcttg aacgggaaac
     1801 gggacttcct gcggcaaggc ggatcgaatt tcgcagttgg ccttgcgtcg gtcctcgccg
     1861 ccgcgattct cggcgcctgc ggaggcggag gctcgtcgtc ttcctcacca cctcctccgc
     1921 cgccgcccaa ccccgcgctg gtgcgcgtct cggcggcttc cccgttcgcg gcaaattgcg
     1981 gcggcggcat gcggggcagc gtgttgtatc aaaacgccga agtcgaaccc ttcgtcgccg
     2041 taaatcccgc gaacccggcc catgtcgtcg gcgtgtggca gcaggatcgc tggtccgacg
     2101 gcggcgccca cgggctcatg gccgccgcct ccttcgacgg cggcaagacc tgggcgccgc
     2161 gggcgctggt catgtcgcgc tgcgcaggcg gcaacgccgg aaacggagcc gattacgccc
     2221 gggcctccga cccctgggtc acaatttcac ccgacggcgc cgcgtacgcg atttcgatat
     2281 cgttcagcgg cgtgactctg caaccgaatt ccacgagttc ggtgctggtc agccgctccg
     2341 cggacggcgg gcaaacctgg agcgatccgc ttaccctcat cctggacggc cccgatgcgt
     2401 tcaacgacaa ggaatccatc gccgccgatc cgaacaacga gaattacgtc tatgccgtct
     2461 gggaccgcct cacgaccgcg aacaccggcg ctgccatgct cgcgcgcacc gcggacgggg
     2521 gcgcgagctg gcagccggcg caggtgctct acgatccggg cgcgaacaac cagacgctca
     2581 acaatcagct tgcggtattg cctgacggca gcgtcatcgc ctttttcacc gagatcgaca
     2641 acgcctttca gggaacgccg aacgcgcata tggcggtcgt gcgttccacc gatcacggcg
     2701 cgacttggtc tgcgccggtc aaagtggcgg acattcacgc cgtcggcgcc cgtgatcccg
     2761 aaaccgggca ggacatccgc gacggagcct ttctcggcca gattgtggcc ggacccgacg
     2821 gaaaactctt cgtcgtgtgg caggacgcgc gcttttccgg gggccagcgc gacggcgtgc
     2881 tgatttcgca atcgggcgac ggcggtctgt cctggtccac gccggtcgag atcaacggcg
     2941 atcccgccgt gcaggccttc gagcccgccg tcgcggtgcg cggcaacggc acgatcggcg
     3001 tgagctacta cgactttcgc gacgacaccg ccgatcgcgc gacgctcccg accgactact
     3061 ggctcacgca atcggcggac ggcgcaagct ggaaggaacg ccggatcgcc gggcctttcg
     3121 acttcgatct cgcgcctgac gccgaaggcc tgttcaccgg cgactatcag ggcatcgtcg
     3181 ctcccgacga catcggcttc ctgccgtttt tcgtgcagac caccgacgag ggaacggcaa
     3241 accgcaccga tgtctttgcg ttcccgcccc gcggcggcag cgagagtctc ggctcggcgg
     3301 tggccgttcg ctccgtcatg cgcgagccct tgccgatgag ccccgagttt cggcggcgcg
     3361 tgcaatcgcg gctgctcgat ctgctccgcg aggaaatccc cgggcgcggc aaacccgagg
     3421 cgccgccggt ccagccgtag cggaagccgt tttcatccgc ggccaaacgc tgcgcgcgcc
     3481 gctggccaag cacgccggtg agagcgtaca ccacaggaac cccttcgcct cttccacgac
     3541 cggtagttcg acgccgcgcc tcttacgtag cttccgctat cgcaacacgg gcgaagcgac
     3601 gcttcccgac ctggatgacg taggttttcc cggcggccag tcgcagttca cggttctcga
     3661 cgcgctcgcc gtccacgcgt accgcgccct gttgaagcat ccggttcgcc tcgctcgtgc
     3721 tcggcaccag ccccgcctcc ttgaggagct tcgcgagcgg atgctcgccg gcgattcgca
     3781 cttcgggcat atcctgcggc gtttcgccgc gcgcgaaccg ggccacgaaa tcttctctcg
     3841 ccttgcgcgc cgcgtccgcg ccgtggaatc gcgtgaccat ctcctccgcc agcaggaact
     3901 tgacgtcgcg cggattgccg ccgtccgcca cttcgagctt gagcacatcc agttcggcaa
     3961 gcgagcgtgc cgagaggagt ccgaaatacc gccacatgat ctcgtcggag atggacatga
     4021 gtttgccaaa catctcgtcg ggcggatcgg tgagaccgat cgcattgccg agcgatttcg
     4081 acatcttttg cacgccgtcg agcccttcga gaatcggcat cgtcatcacg acctgcggcg
     4141 tctgaccgtg ggcggtttgc aattggcgcc cgacgagaag attgaagcgc tggtcggtgc
     4201 caccgagttc gatatcggcg cggagtgcga cggaatcgta gccctgcgcg agcggataga
     4261 gaaattcgtg gagcgagatc gggctttccg cacgatagcg ctgttcgaaa tcgtcgcgct
     4321 cgagcatgcg ggcgacggtc atgtgccccg cgagacgcac gagatcggcg gcggagagct
     4381 tgcccagcca ttcgctgttg aaggcgatgc gcgttttcgc gagctcgaga atgctgccga
     4441 cctgagcgcg gtaggtctcg gcgttcgccg caatgtctgc cgggccgagc gcgggacgcg
     4501 tcgcgctccg cccggacggg tccccgatca tgccggtgaa atcgccgatg acgaacacga
     4561 tcgcgtgacc gcgctcttgg aactggcgca gcttgttcag gatcaccgta tggccgagat
     4621 gaagatcggg agccgtgggg tcgaaaccgg ccttgacgac gagcgatcgc ccggccttca
     4681 gccgctcgtc gagttcctcg cgcttgatta cgtcggccgc gccgcgtacg agctccgcga
     4741 gatcgggatg ttcgcaattc gctgccacgc tgccgcagtc ccggagggtc gctgaattat
     4801 aacgatccgg cggcgcagag acggccggaa caaggacggg gctcagcgct ttcgcggcag
     4861 gtaaagatcg gtgatggtgc cggcggcgat ctcggcggcc atgccgacgc tttcggcaag
     4921 cgtcgggtgc ggatggatgc tgagcgcgat gtcttcggca tcgcagccca tctcgatcgc
     4981 gagcaccagc tcggaaatca gttctccggc gctgggcccg acgatcccgg cgccgacgat
     5041 gcgcccgctc gcgcggtcga agagcagctt ggtgaagccg aaatccacgc ccagggtcag
     5101 tgcgcgaccg tttgcctgcc atggaaagac gccgcgctcg atctcccttc cggttttttg
     5161 cgcttcggtt tccgtgagcc ccgcccaggc gacttccggg tgcgtgtagg cgaccgcggg
     5221 gatcgcgcgc acgtcgaagg cggacttccc gccgcaggcg acctcggccg caaccttgcc
     5281 ttcatggctc gccttgtggg cgagaagcgg cgggccggcg atgtcgccga tcgcgaagat
     5341 gtgtttgacg ttgctgcgca tctgttcgtc cgtggggatg aacccgtgtt cgtcgacggt
     5401 gatgcccgcg gcctccgcgc cgatcgcgtg cccgttcggc cggcggccga tcgcgaccag
     5461 cacgcgctcg aaaccggcgc tccattcgcc ccgggggccg atgcaacgca cgtgaacgcc
     5521 gtcctcctgc ggttcgattc cggcgacacg cgtttcgaga tggatggcgt cgaagagctc
     5581 ttcgagccgc ttcgcgagcg gcttgacgag atcgcgatcg gtgccgggca tgagtccgcc
     5641 ggtaagttcg accagcgtga ccgcggcgcc gagcgcggca tacacctgcg ccatttccag
     5701 cccgacgatc ccgccgccga cgacgaggag tttcttcggc acttccgcga gttcgagcgc
     5761 gcccgtggaa tccatgatcc gcgggtcgtc cggctgctcc gggagccgca tggcctcgga
     5821 tccggcggcg acgatcgcct gcgcgaaggc gatcgtctgc tttttcctgt cggtctccac
     5881 ttcgagaagc tgcggggaga cgaagcgtgc gttgccgata acgacccgga ccttgcgctt
     5941 tttcgcgagc tcggcgagac cgcccgtcag ccgatccacc gccgtctgct tgaagcgaag
     6001 aagatcatcg agccgaatct cgggcgcgcc gaagacgatg ccgtgcgcgc tcatcgcgtg
     6061 cgcct
//
LOCUS       DASUJG010000290         4100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_451857, whole genome shotgun sequence.
ACCESSION   DASUJG010000290 DASUJG010000000
VERSION     DASUJG010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451857"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3009
                     /gene="mfd"
                     /locus_tag="VFX38_06240"
     CDS             <1..3009
                     /gene="mfd"
                     /locus_tag="VFX38_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX5314487.1"
                     /translation="FDPDTQLSGARIENITLLPAYEFPFDEESIGKFRQAWRARFAGD
                     PMTSEIYRAVSAGRIPQGVESWLPLFFETTDTLGDYLPPMTSVLTFDGFDAALDAAWT
                     QIEARHESARHDIERPLLTPAEAFAEPQTVRDSLLGYARTPINNSSATTSPLAAAATP
                     SSPSPFKGEAWGEGEIQQATRRSGAAPHPTPLPAERGEGANRQASRPVEFDALPELAL
                     DAKAEEPLERFLTFAAHTPALLFTAEAPGRRETVRELLARRGLEPELIEGWQAFRERR
                     PALALAVAELEGGFVLPNDGIAVVPDAAVFGARAASRRRQRRAVRDPATLIRDLTDLR
                     EGAPVVHQDHGVGRFRGLVTRDVDGQAIEFVHLEYAEGDRLYVPVAALDRLSRYLGAD
                     AEHAPLTRLGSGQWERARQRAAARARDTAAELLELYARRAARAGHAFEFDAAEWERFV
                     AAFPFDETPDQQRAIAEVVDDLRSDRPMDRLVCGDVGFGKTEVALRAAFVAVEAGRQV
                     AVLVPTTLLADQHYRNFNDRFADWPVRVELLSRFRSAKQQAEVRAALTDGRVDIVIGT
                     HALLNRGIRFKALGLVIVDEEHRFGVRHKERLKALRAEVDVLTLTATPIPRTLSMTLA
                     GIRELSLIATPPEERLPIKTVVSQWQDAQIRDALLREARRGGQVFVVHNEIRGIGELA
                     ERIRRLVPEASVRVGHGQMPERELESLMVDFYHRRFDVLVSTTIIESGIDIPTANTIL
                     IHNAHRFGLAQLHQLRGRVGRSRHQAYAYLITPPLDLLGREARARLDAVASMEELGAG
                     FFLATQDMEIRGAGALLGENQSGQIEEVGLELYNRLLARAVATLKAGGEVDPEVGFEA
                     GCEVDLGATALLPADYVPNVHLRLVLYKRIAGAANDEELETLTAEIVDRFGAPPEAAQ
                     RLLEITRIKQRAAALGIESMHAGPTGARLDFGPTPAIDANALVRLIQSNPKAYRLQGQ
                     KRLTVRRDLPDFAHRLEAITHALTTLTPAA"
BASE COUNT          744 a         1512 c         1147 g          697 t
ORIGIN      
        1 ttcgatcccg acacccagct ttccggtgcg cgcatcgaaa acatcacgct ccttccggcc
       61 tacgagtttc cgttcgacga ggaaagtatc ggcaaattcc gccaggcctg gcgcgcgcgc
      121 ttcgcgggcg atccgatgac gagcgaaatc tatcgggccg tttccgcagg acggattccg
      181 cagggcgtcg agagttggct gccgctcttt ttcgaaacca ccgacacgct cggcgactat
      241 ctgccgccga tgacttccgt cctcacgttc gacggtttcg atgccgcgct cgacgcggcc
      301 tggacgcaaa tcgaagcgcg ccacgaatcg gcacgccatg atatcgagcg cccgctgctc
      361 acgcccgcgg aggccttcgc cgaaccgcaa accgtccgcg actcacttct cggctacgcc
      421 cgaacaccga tcaacaattc ttccgcaaca acctctccac tggccgcagc ggccactcca
      481 tcttccccct ctcccttcaa gggagaggct tggggtgagg gtgaaataca acaggcgacg
      541 cgtcggagcg gtgccgcccc tcaccccacc cctctccccg cagaacgggg cgagggagca
      601 aacagacagg cctctcgccc agtcgaattc gatgcattgc cggaactggc actcgacgcc
      661 aaggccgagg aaccactgga gcggttcctc acctttgcgg cgcacacgcc agctctgctc
      721 ttcaccgccg aagcgcccgg ccgtcgtgag accgtgcgcg aactcctcgc ccgccgcgga
      781 cttgaaccag aactcattga aggctggcag gcgtttcgcg agcggcgccc cgctctcgca
      841 ctcgccgtcg ccgaactcga aggcggtttc gtcctgccga acgacggcat tgccgtcgtg
      901 ccggatgctg ccgtgttcgg cgcgcgcgcc gcgagccgcc gccggcagcg ccgcgccgtg
      961 cgcgatcccg cgaccctcat tcgcgacctc accgatctac gcgaaggcgc accggtcgtg
     1021 caccaggatc acggcgtcgg gcgctttcgc ggcctcgtca cccgcgacgt ggacggtcag
     1081 gccatcgaat tcgtgcatct ggaatacgcc gaaggcgacc ggctttacgt gcccgtcgcc
     1141 gcgctcgatc ggctctcgcg ctatctcggc gccgacgccg aacacgcgcc gctcacccgc
     1201 ctcggcagcg gccagtggga acgcgcccgg caacgcgccg ccgcacgggc ccgcgacacc
     1261 gcggcggaac tgctcgaact ctacgcgcgc cgcgcggccc gcgccggtca cgccttcgag
     1321 ttcgacgcgg cggaatggga acgtttcgtc gccgcgtttc cgttcgacga aacgcccgat
     1381 cagcaacgcg ccatcgccga agtcgtggac gatctcagga gcgatcgacc gatggatcgg
     1441 ttggtctgcg gcgacgtcgg cttcggcaag accgaagtgg cgctccgcgc cgccttcgtc
     1501 gccgtcgagg ccggacgcca ggtcgcggta ctggtgccga cgacgctcct cgccgatcag
     1561 cattaccgca acttcaacga tcgcttcgcc gactggccgg tgcgcgtcga actcctttcc
     1621 cgctttcgca gcgccaaaca acaggccgag gtgcgcgccg cgctcaccga tgggcgcgtg
     1681 gacatcgtca tcggcaccca tgcgcttctc aatcgcggca tccgcttcaa ggcgctcggt
     1741 ctcgtgatcg tggacgagga acaccgcttc ggcgtgcgcc acaaggaacg gttgaaggcg
     1801 ctgcgcgccg aagtggacgt actcacgctc accgcgaccc cgatcccgcg cacgctctcg
     1861 atgacgctcg ccggaattcg cgaactgtcc ctgatcgcga ctccgcccga ggaacgcttg
     1921 ccgatcaaaa ccgtcgtcag ccagtggcag gacgcgcaaa tccgcgatgc gcttctgcgc
     1981 gaggcccgcc gcggcggcca ggtgttcgtg gttcacaatg aaatccgcgg catcggcgaa
     2041 ctcgccgaac gcatccgccg cctcgtgccg gaagcttcgg tgcgcgtcgg ccacggtcag
     2101 atgcccgagc gcgaactgga atcccttatg gtggacttct atcaccgccg cttcgacgtg
     2161 ctcgtctcga ccaccatcat cgaaagcggc atagacattc cgaccgccaa caccatcctc
     2221 atccacaacg cgcaccgctt cggcctcgcg caactgcatc aattacgcgg gcgcgtcggc
     2281 cgttcgcgcc accaggccta cgcctacttg attaccccgc cgctggactt gctcggacgc
     2341 gaagcgcgcg cgcggctcga cgccgtcgca agcatggaag aactcggcgc cggattcttt
     2401 ctcgcgaccc aggacatgga aatccgcggc gcgggcgcgc tgctcggcga gaatcaaagc
     2461 ggacagatcg aggaagtcgg cctcgaactc tacaaccgcc tgctcgcccg cgccgtggcg
     2521 acgctcaagg ccggcggcga agtggatccc gaggtcggat tcgaagccgg ctgtgaggtg
     2581 gacctcggcg cgaccgcact cctgcccgcc gactacgtgc ccaacgttca tctgcgtctg
     2641 gtgctctaca agcgcatcgc cggcgccgca aacgacgaag aactcgaaac tctcaccgcc
     2701 gaaatcgtgg accgcttcgg cgccccgccc gaagccgccc aacgcctgct cgaaatcacg
     2761 cgcatcaaac aacgcgccgc cgcactcggc atcgaaagta tgcacgccgg ccccaccggc
     2821 gcccgcctcg acttcggccc cacacccgcc atcgacgcaa acgccctcgt gcggctgatc
     2881 caaagcaacc caaaagccta ccgcctccaa ggccaaaaac gcctgaccgt tcgccgcgac
     2941 ctccccgact tcgcccaccg cctcgaagcc atcacccacg ccctcaccac cctcaccccc
     3001 gccgcttgaa tcaccgcgcc atgtaggttg ggctgagcaa cgcgaagccc aacatccttc
     3061 cgcgcgccaa cgttgggctt cactgcgttc agcccaacct actcgcttcg ccgaccgcct
     3121 cgaagccatc atccacgtcc tcaccaccct cacgcctcca cccaaaccct gatccccatt
     3181 tcgcttgccg atcaaccgca tggcagcctt ttgactgtcc gttcccgcag gtatcaattc
     3241 ctccgatacg gcgctataat ccgccgtgct tatacgtcag ttcgccggat tggccgctac
     3301 tttgacgtct agttagcgtt agatattcag cctatggagg tggcacgtga gtaggtgggt
     3361 aattgttacg ttcatgttgg gcggcataat tggaatcgcc ggctgtacgg ttaatcttca
     3421 gcccgtggcg ccaacagtct cgccgaccac cgcccgcgac agcgcccaac agcttccgtg
     3481 cagtgctgtt cttcttctac ccgccagttt ccgcgacgag tcgtacgctt cctcattcgg
     3541 tactcgccgg attaccgtta ctcttggtcc acccgcggta acggcggtgg gggagcttgc
     3601 ggaagaccgc ttcacttccg tcaccaaagt acccgtcccg ggtgacggga ctatcgcgtt
     3661 catgcaacga acatcatcgg cccccgccgc caccctcata ctgaggccgc gctttcagca
     3721 agtcagctca tccccaggct cgttccacta cggaataacg ctcagtgtag cgctggacat
     3781 agccggccgc ggcgtggctc atactgtagt cggtagcgga acaggcaccg gccccgtgta
     3841 ctcacggtca gtcctgcagc gcaccgtgaa tactgcattg gcccgtgcaa ttgcaagcct
     3901 cgcaaggagt ttccaaccga cttgcccggc gcttggcgcg gacagcgacc caacacaata
     3961 agcccccgct actcccatcc aactattcgc ctaaccggac gttcacccgc tgcgcgggtt
     4021 cacgctggtc acctcaagcg ggaattcagg gtcaagttga gctaccccgg cttttccgga
     4081 catcaaacag aggggacaat
//
LOCUS       DASUJG010000291         6703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1430069, whole genome shotgun sequence.
ACCESSION   DASUJG010000291 DASUJG010000000
VERSION     DASUJG010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6703
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1430069"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..1068
                     /locus_tag="VFX38_06245"
     CDS             295..1068
                     /locus_tag="VFX38_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011399051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5314488.1"
                     /translation="MYKAFGDLVVLDDVSFFVNPGETLCILGRSGVGKSVALQHIMGF
                     LKPDSGRIMVAGEDITGFDEKQLQAIRRKVTMVFQNGALFDSLTVGENVAFPLREHTD
                     LSEPLIRTLVLMTLQAVGLRAAADQAPGELSGGMARRVALARAIVMDPEFIFYDEPFT
                     GLDPISLGAIVCLIRRLNEALGITSIVVSHDVGVIGHVADFSYLIANGKVVARGTPEA
                     LKEDRSSWVHQFMNGLVEGPVPFHLPAPDYAGSLLEEEP"
     gene            1068..1850
                     /gene="mlaE"
                     /locus_tag="VFX38_06250"
     CDS             1068..1850
                     /gene="mlaE"
                     /locus_tag="VFX38_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007641646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEX5314489.1"
                     /translation="MPTIPVELTRRVRTSLAAFGAEWIFFARVMSLFPRALARPVLVI
                     EQIYSLGNRTLALVGFAGLFVGLVIALQLYGVLGRFGATSALGPIVAITLLRELGPVM
                     AALLYAGRAGTALASEIGLMRATDQLSAMEMMAVDPVRRVVAPRFVGGVIAVPLLTLA
                     FNVIGIFGAWLIAVRMMQMDPGVFWGQMHSAVDFMGDFINGIIKSFIFGLAVNLIAVH
                     EGFNARSTADGVARATTRTVVVSSLTVLALDFVLTAFMFQGV"
     gene            1853..2338
                     /gene="mlaD"
                     /locus_tag="VFX38_06255"
     CDS             1853..2338
                     /gene="mlaD"
                     /locus_tag="VFX38_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEX5314490.1"
                     /translation="MRRTFGLELGTGLFVLLGIAAVFFLATQTTNLHALNQGETYEVR
                     AAFSNVGGLKVSAPVALAGVQIGRVSSITLDPQSLQAVVTMRIQKRYDEIPVDSSAAI
                     QTRGLLGDQYIGITPGGSLQSLKSGDTVHFTQSAIVLENLISQFMFHSASSGGKSNGG
                     P"
     gene            2433..3128
                     /locus_tag="VFX38_06260"
     CDS             2433..3128
                     /locus_tag="VFX38_06260"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5314491.1"
                     /translation="MIRERGILAPPWQSVFQHSAKFLAVAILALGLAVAARAAPAPGD
                     PTTVVQQASTDIIGALNKNADKLKSDPALAGQLVKQYLLPHFDFDFTCQLVLGRYWRT
                     ATPAQRSEFQKVFLQYLTTTYAKGLQNFNGAHVNVLPFRGDASQQYVKVRTQVQTAGH
                     APIEVDYALRQTAQGWKAFDVIIAGVSYVQTYQNEFQSEIQRTSLPSLIARLQKAQAP
                     AAASGAPAAASGG"
     gene            3125..3451
                     /locus_tag="VFX38_06265"
     CDS             3125..3451
                     /locus_tag="VFX38_06265"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5314492.1"
                     /translation="MRVGRAASARLIAEGEGRFRVEGEVVFATVMKLLEDGEAAFAGS
                     SRIEIDLGGVTRINTAGLGLVIEWLRRARHEGQTITIRRPPATLLALAQICEAQTILG
                     PVIVPA"
     gene            complement(3469..4266)
                     /locus_tag="VFX38_06270"
     CDS             complement(3469..4266)
                     /locus_tag="VFX38_06270"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEX5314493.1"
                     /translation="MNARSGHASPAILVAALALAALLAGCATRPGNPGNVPAAKRDPL
                     APANRQVYRFNHLLDSAVLRPAAKGYVKVTPHPVRSGITNFFHNLEMPVTIVNDLLQL
                     KPLDAVRDTGRFFTNSTAGLAGFLDPASNWGMPPHSEDFGITLARWGVPSGPYLQIPF
                     LGPSDVRDAFGLYVDHFASAFYWTGWSAGTRNAIYALNAVQLRAQFLSLDQLLEQAYD
                     PYAFMRSAYFQNRDQTVRQNLPNYNPEQLPDYQQMLGPDPNGGGDRP"
     gene            complement(4263..5444)
                     /locus_tag="VFX38_06275"
     CDS             complement(4263..5444)
                     /locus_tag="VFX38_06275"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX5314494.1"
                     /translation="MHFRAALACGGTTADEHCQLAAAAGQFGDFVTLRREARAAIAAD
                     RRWYTAWYQLSITPDEAAAETMQAMRAAAVEAGADPAAWPLHLALARVLEKAGRYDEA
                     FAAADEANRRRVRALRLDYGDDWRYFANVRRCLGKEFASGALPAPTRGPRPIFIVGMP
                     RSGTTLVEAILGAHPAVTPGGEMQFLHLWLHRNLGAADQSQIPERLATAPPAALLRLA
                     RDWREFLDRSCAARAWISDKMPENYALLGLIARCFPEAAIVHVRRDPRDTCVSCFFTA
                     LAGRGLPASLAEVGAWYRAYEGLMTHWRDVLGAERIVEVEYERLVSSPEPEIRRLLAA
                     IGLDWHSGCLAYYASPKTVATASLYQVRQPPYRDSIGRWRHFERHLLPLRRALAGEAA
                     S"
     gene            complement(5543..>6703)
                     /locus_tag="VFX38_06280"
     CDS             complement(5543..>6703)
                     /locus_tag="VFX38_06280"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX5314495.1"
                     /translation="LVAKIRGQLDAALHQYRAVLERRPDYPGYAQLASLKHFSVDDAD
                     IALLERAFGRLTPDASPEVRADLLFALAKACDDAERIEEASRYLVEANRIERERRPYD
                     PAADEARMLRIQELFTREFIERYRGGGLTGLQPIFVVSLPRSGSTLTEQMLAAHSAIR
                     GGGEIGHFLRVATALSLKWGARADFPRLDETVAGEDLREAARDYAKLTVTLRLLTPRF
                     TDKTLVNFLYVGLIAMMLPDAKIIHVRRHPLATVLGIYRQHFARGLAYSFDLDAIVAY
                     YRAYAQLMRHWRRVAADAFTEIFYERLIADPEAELGRALDYVGLEFEPRCLEFYRLDR
                     PVRTASVIQVRQPLNRQGLERHQRYEKLLAPVATALADEIAAYEAELAAAGH"
BASE COUNT         1125 a         2133 c         2287 g         1158 t
ORIGIN      
        1 tggagcgggc gggagcgggc acggggctgc gcattgacag cttcgacgcg gagtccattg
       61 cggcctcgca gctctgggag cagcggcttc cggtgtattg ctggttccgg aacctgaatt
      121 cgccggagga tttcgagacg gcgttggagc aatcccggcg tatcccgtaa gctgaaggca
      181 gaggctttcg ccggcatttg aaggagcgga ccgctgtcca ccggaatcga gatcgaaaca
      241 atcattgaag agcccgcggg ccacgcgccg gggccatata tcgcctttga aaacgtgtat
      301 aaggcgttcg gcgacctggt ggtgctggac gatgtgagct tcttcgtcaa tccgggcgaa
      361 acgctctgca ttctgggccg cagcggcgtg ggaaagtcgg tcgcgctgca gcacattatg
      421 ggattcctga agccggactc gggccggatc atggttgcgg gcgaggacat taccggcttc
      481 gacgaaaagc agttgcaggc gatccggcgc aaggtcacga tggtgttcca gaacggcgcg
      541 ctgttcgact cgctgacggt gggcgagaac gttgcctttc cgctgcgcga gcacaccgac
      601 ctttcggaac cgctgatccg cacgctcgtt ctcatgaccc tgcaggcggt cggcctgcgg
      661 gcggccgccg atcaggctcc cggcgaactc tccggcggca tggcgcggcg cgtcgccctc
      721 gcccgcgcga tcgtgatgga ccccgagttc atcttttatg acgagccctt caccgggctc
      781 gatccgattt cgctcggcgc catcgtgtgc ctcattcgcc ggctcaacga ggcgctcggc
      841 atcaccagca tcgtggtctc gcatgacgtc ggcgtgatag gacacgtagc ggatttctcg
      901 tacctgatcg cgaacggcaa ggtcgtcgcc cgcggaactc ccgaggcgct caaggaggat
      961 cgctcgagct gggtgcatca attcatgaac ggcctcgtcg agggtccggt tcccttccat
     1021 ctgccggctc ccgattacgc cggttcgctc ctcgaggagg agccctgatg ccgacgattc
     1081 cggtcgaact cacgcgccgg gtgcgcacct cgctcgcggc cttcggggcg gagtggattt
     1141 tcttcgcccg cgtaatgtcg ctctttccgc gggcgcttgc acgcccggtg ctggtgatcg
     1201 agcagattta ttcgctcgga aaccgcaccc tcgcgctcgt cggcttcgcc ggtctgttcg
     1261 tcggactggt gatcgcgctt caactctacg gagtactcgg gcgcttcggt gcgacctcgg
     1321 cgctcggtcc gatcgtcgcg atcacgctgc tgcgcgaact cggcccggtg atggcggcgc
     1381 tcctctacgc cgggcgggcg ggcaccgcgc tcgcgagcga gatcgggctc atgcgcgcga
     1441 ccgatcagct atcggcgatg gagatgatgg cggtggaccc ggtgcgccgc gtcgtcgcgc
     1501 cgcgcttcgt cggcggggta atcgcggtgc cgctcctcac gctcgccttc aacgtcatcg
     1561 ggattttcgg cgcctggctc atcgccgtgc gcatgatgca gatggatccc ggggtgttct
     1621 ggggccagat gcattcggcc gtggacttca tgggcgactt catcaacggc atcatcaaga
     1681 gtttcatctt cggcctcgcg gtcaacctga tcgcggtgca cgagggcttc aatgcccgct
     1741 ccactgccga cggtgtggcg cgtgcgacca cgcgtaccgt ggtggtgtcc tctctcaccg
     1801 tgctcgcgct cgacttcgtg ctcacggcct tcatgtttca gggagtgtaa cgatgcgacg
     1861 taccttcggc ctcgagctgg gcacggggct cttcgtgctg ctcggcattg ccgccgtgtt
     1921 ttttctcgcg acccagacga ccaatctgca tgccctgaat cagggggaaa cctacgaggt
     1981 gcgcgccgcc ttcagcaacg tcggcgggct caaggtttcg gctccggtcg cgctcgccgg
     2041 ggtgcagatc gggcgcgtga gttcgatcac gctcgacccg cagtcgctgc aggcggtcgt
     2101 gacgatgcgc atccagaagc gctacgatga aattccggtc gattcgagcg ccgcgatcca
     2161 gacccgcggg ctcctcggcg accagtacat cggcattacc cccggcgggt cgctgcaatc
     2221 gctcaagagc ggcgacaccg tccatttcac ccagtcggcg atcgtgctgg agaatctgat
     2281 cagtcagttc atgttccaca gcgcatcgtc aggaggaaag tccaatggcg gtccataaca
     2341 gcttgttgaa aaagggctgt cctgcccttt ttcaaggtcg cgaatccggc acatgtccgg
     2401 attcgctcac gacgaatcaa tcgcgtacgc gcttgattcg cgagcgggga atccttgccc
     2461 cgccttggca gagtgttttt caacactccg ccaagtttct cgcggttgcg attctcgccc
     2521 tcggcctcgc cgtcgcggct cgcgccgcgc ccgcgccggg agatccgacg acggtggtgc
     2581 agcaggcttc caccgacatc atcggcgcgc tcaacaagaa cgcggacaag ctgaagagcg
     2641 acccggctct cgcggggcaa ttggtgaagc agtaccttct gccgcatttc gacttcgact
     2701 tcacctgcca gctcgttctc ggccgctact ggcggacggc gacgcccgcg cagcgcagcg
     2761 aattccagaa agtcttcctg cagtatctga ccaccaccta cgccaaggga ctccagaact
     2821 tcaacggcgc ccacgtgaac gtgctgccct tccgcggcga tgcctcgcag cagtacgtga
     2881 aggtgcgcac ccaggtgcaa accgccggtc atgccccgat cgaggtggac tacgcgctgc
     2941 ggcagaccgc gcaaggatgg aaggccttcg acgtcatcat cgccggggtt tcctacgtcc
     3001 agacctacca gaacgaattc cagagcgaga ttcagcgcac cagtctcccg tcgctgatcg
     3061 cacgcctgca gaaggcacag gcgcccgcgg cggcgagcgg ggcgccggca gctgcgagcg
     3121 gcggatgaga gtcgggcgcg cggcgagcgc gcggctgatc gcggagggcg agggccgctt
     3181 tcgcgtcgaa ggcgaggtgg tcttcgcgac ggtgatgaaa ctgctcgagg acggcgaggc
     3241 cgcgtttgcc ggatcatcgc gcatcgaaat cgatctcggc ggcgtcacgc gcatcaacac
     3301 cgccggcctc gggctcgtca tcgagtggct gcgccgcgcg cgtcacgagg ggcagacgat
     3361 cacgatccgt cgcccgcccg cgacactgct cgcgctcgcc cagatctgcg aagcccagac
     3421 gattctcggc cccgtcatag tcccggcctg acatcggcgc gaggattctc aggggcggtc
     3481 gccgcctccg ttcggatcgg gacccagcat ctgctggtaa tcgggcaact gctcgggatt
     3541 gtagttcggc agattctggc gaacggtctg gtcgcggttc tggaagtagg cgctgcgcat
     3601 gaaggcgtag ggatcgtagg cctgctccag caattgatcg aggctcagga attgcgcgcg
     3661 cagttgcacc gcgttgagtg catagatggc gttgcgggtt ccggcgctcc agccggtcca
     3721 gtagaaggcc gatgcgaagt gatccacgta gagaccaaag gcatcgcgca catcgctcgg
     3781 gccgagaaag ggaatttgca gatacggtcc gctcggcact ccccagcgcg cgagggtgat
     3841 gccgaaatcc tcgctgtgcg gcggcatgcc ccagttcgag gcagggtcga gaaaacccgc
     3901 gagtcccgcc gtcgagttgg tgaaaaagcg tccggtgtcg cgcaccgcat cgagcggttt
     3961 cagttgcagc agatcgttca cgatcgtgac cggcatctcg aggttgtgga agaagttcgt
     4021 gatgccgctg cgcaccggat gcggcgtcac cttgacatag cccttggcgg ccggacgcag
     4081 cacggcagaa tcgagcagat gattgaagcg ataaacctgg cgattcgcgg gcgcgagcgg
     4141 atcgcgtttg gccgccggca cgttgcccgg attgccgggc cgggtcgcgc agcccgcgag
     4201 caaggcggcg agggcgagcg cggcgacgag aatcgccggg gaggcatgtc cggagcgtgc
     4261 gttcaagaag cggcctctcc cgcaagagct cggcgcaagg gtaacagatg gcgctcgaag
     4321 tgtcgccagc gtccgatcga atcgcgatag ggaggctggc ggacctgata caggctcgcc
     4381 gtcgcgaccg ttttcggcga ggcgtaatag gcgagacagc cggaatgcca gtcgaggccg
     4441 atggctgcca gcaggcggcg gatttcgggt tccggcgagg aaaccagccg ttcgtattcc
     4501 acctcgacga tgcgctcggc gccgagcacg tcgcgccagt gagtcatcag gccttcgtag
     4561 gcgcggtacc aggcgccgac ttcggcgagg ctcgccggca ggccgcgacc ggcgagcgcg
     4621 gtgaaaaaac aggacacgca agtatcgcgg gggtcgcggc gcacatgcac gatcgccgcc
     4681 tctgggaaac agcgggcgat gagtccgagc agggcgtaat tttccggcat cttgtcgctg
     4741 atccacgccc gggccgcgca ggagcggtcc aggaattcgc gccagtcgcg ggcgaggcgc
     4801 agcagcgcgg ccggcggagc ggtggcgagg cgttccggaa tctgcgactg atcggcggcg
     4861 cccaggttgc ggtgcagcca gaggtgaaga aactgcattt ccccgccggg cgtgacggcg
     4921 gggtgcgccc cgagaatggc ctcgaccagc gtcgtccccg agcgcggcat gccgacgatg
     4981 aagatcgggc gcgggccgcg cgtcggggcc ggcagtgccc cgcttgcaaa ttccttccct
     5041 aggcaacggc gaacgttggc gaagtagcgc cagtcgtcgc cgtaatcgag ccggagcgcc
     5101 cggacacggc gccgattggc ctcatcggcc gcggcaaagg cctcgtcgta ccggcccgcc
     5161 ttctccagca cccgggcgag ggcgaggtgc aggggccagg ccgccggatc ggcaccggcc
     5221 tcgacggccg ccgcgcgcat cgcctgcatc gtctccgcgg ctgcctcgtc gggcgtgatg
     5281 gacagttggt accaggccgt ataccagcga cgatcggcgg cgatggccgc gcgcgcctcg
     5341 cgccgcagcg taacgaagtc gccgaactgg cccgcggccg ccgccagttg gcagtgttcg
     5401 tcggccgtcg ttcccccgca ggcaagcgcc gcgcggaaat gcatcgcggc tgccgcgaca
     5461 tccccctgac ggcgtgcttg agtacaggcc cggagcagcg ccagaaggcg ggaatcgccg
     5521 gcgccgccgg atggtgcgga ggtcaatggc cggcggccgc gagttcggcc tcgtaggcgg
     5581 caatctcgtc ggcgagtgcc gttgccaccg gagcgagaag tttctcgtag cgctggtgcc
     5641 gctcgaggcc ctgccgattc agcggctgcc gcacctgaat gacgcttgcg gtgcgtaccg
     5701 gacgatcgag ccggtaaaat tcgaggcagc gcggttcgaa ttcgagcccg acgtagtcga
     5761 gcgcccgccc gagttccgct tccggatccg caatcaggcg ttcatagaag atttcggtga
     5821 aggcgtcggc cgcgacccga cgccagtgac gcatgagttg cgcataggcc cggtagtacg
     5881 cgacgatcgc atcgaggtcg aagctgtagg cgagcccgcg cgcaaaatgc tggcgataga
     5941 tcccgagaac ggtagcgagc gggtgacggc gcacgtggat gatcttcgca tcgggaagca
     6001 tcatcgcaat gaggccgacg tagaggaaat tgaccagcgt cttgtcggtg aagcgcggcg
     6061 tgaggaggcg cagcgtcacg gtgagcttgg cgtagtcgcg cgccgcctcg cggagatctt
     6121 cgccggcaac cgtctcgtcg agccggggga aatccgcgcg tgcaccccac ttcaggctga
     6181 gcgccgtggc cacccgcagg aaatgtccga tttcgccgcc cccgcggatg gccgaatgcg
     6241 cggcgagcat ctgttcggta agcgtcgagc cggagcgcgg cagcgacacg acaaagatcg
     6301 gctgcaatcc ggtgaggccg cctccccgat aacgctcgat gaactcccgc gtgaagagtt
     6361 cctggatgcg gagcatgcgc gcctcgtccg ccgccggatc gtacggccgc cgctcgcgct
     6421 cgatgcggtt ggcttcgacg aggtaccggc tcgcttcttc gattcgttcg gcatcgtcgc
     6481 aggccttggc gagcgcgaac aggagatcgg cccgcacctc cggggatgca tccggcgtga
     6541 ggcgtccgaa agcacgctcc aacagggcga tgtcggcgtc gtcgaccgag aagtgcttga
     6601 gcgaggcgag ctgggcgtag ccggggtaat ccggtcggcg ctccagcacc gcacgatact
     6661 ggtgcagggc cgcatcgagt tgcccgcgga ttttggcgac cag
//
LOCUS       DASUJG010000292         2223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1553260, whole genome shotgun sequence.
ACCESSION   DASUJG010000292 DASUJG010000000
VERSION     DASUJG010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1553260"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VFX38_06285"
     CDS             <1..202
                     /locus_tag="VFX38_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314496.1"
                     /translation="ELRDGVDLYINPPVRAYGFLDWNAIYSLVDVGYRHAQACLQSWL
                     DQHPELRWREDFAAPAAETVTA"
     gene            199..1101
                     /locus_tag="VFX38_06290"
     CDS             199..1101
                     /locus_tag="VFX38_06290"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5314497.1"
                     /translation="MIDETSRVDDLAACGHRGSALVALHTAVALFGFAALFGQWIALP
                     ATAIVLGRTTTAAATLAIVARLRGQRIGRGDRRLAANGAILASHWVSFFAAVKIAGVP
                     IALLGYAVFPVFVLLLERPHRSSGIREWTTALLATAGLVALVPELSWANHATRGLAWA
                     LVSAFTFAWLTVRNRALVATRSATGIALWQNLFAAVCLLPWVVAFDGRAALPAPIDIG
                     LLLVLGTLCTGLSHTLFIASMRRVSAHTASVVAALEPVYGIVLAALLLGERPGSRTIM
                     GGVLIVAAALNASLRTEVGEARGV"
     gene            1139..2053
                     /gene="gnd"
                     /locus_tag="VFX38_06295"
     CDS             1139..2053
                     /gene="gnd"
                     /locus_tag="VFX38_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEX5314498.1"
                     /translation="MDIGLVGLGRMGGNMARRLARGGVRVIGFDPDERVRRSLADDIV
                     EARSLHDVVDALTAPRIVWLMVPAGDVTQRTVDELAALLAPGDLLVDGGNANYHDSQR
                     RGLALAARGIRFVDCGVSGGVHGLANGYALMFGGDEKAAAMLLPYVRILAPAADAGWL
                     HCGPVGAGHFVKMIHNGIEYGMMQALAEGFALMHKRTDLALDVAKIAQMWRHGSVVRS
                     WLLDLTAEFLAQDAELHDIAPIVADSGEGRWTVQEAIEQGVPAPVLSLALMARFSSQG
                     GGDYANRMLAMMRKGFGGHAVASADDKR"
     gene            2034..>2223
                     /locus_tag="VFX38_06300"
     CDS             2034..>2223
                     /locus_tag="VFX38_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEX5314499.1"
                     /translation="MQTTSDEGQVEGMIVVIMGVSRSGRSTVGAALARDLRFVHLRGD
                     AETIAARLATRQHRCMPAT"
BASE COUNT          298 a          742 c          770 g          413 t
ORIGIN      
        1 cgagcttcgc gatggcgtcg acctgtacat caatccgccg gtgcgcgcgt acggctttct
       61 cgactggaac gcgatctact cgctcgtcga tgtcggctat cgccacgcac aggcgtgcct
      121 gcaatcctgg ctcgaccagc atcccgagtt gcggtggcgg gaggacttcg cggcgccggc
      181 ggccgaaacc gtgaccgcat gatcgacgag acctcacggg tcgacgacct ggccgcgtgc
      241 ggtcatcgcg ggtccgcgct cgtcgcgttg cacacggcgg tcgcgctgtt cggttttgcc
      301 gcgctcttcg gccagtggat tgcattgccg gcgacggcga tcgtgctcgg ccgcacgacg
      361 acggcggccg cgacgctcgc catcgtcgcg cgcctccgcg gtcagcggat tggtcgtggc
      421 gaccggcggc ttgcggccaa cggcgccatt ctcgcgtcgc attgggtgag cttcttcgcg
      481 gcggtgaaga tcgccggcgt cccgatcgcg ttgctgggct acgcggtctt tccggtcttc
      541 gtgctgctgc tcgagcgtcc gcaccgctcg agcggcattc gcgaatggac gacggcgttg
      601 ctcgcgaccg ccggcctcgt ggcgctcgtc cccgagcttt catgggcgaa ccatgcgacg
      661 cgcgggcttg catgggcgct cgtgtccgcc ttcacgtttg cctggctcac ggttcgcaat
      721 cgtgcgctcg tcgcaacacg ttccgcgacc ggcatcgcac tgtggcagaa cctcttcgcg
      781 gccgtctgcc tgttgccatg ggtagtcgct ttcgacggac gggccgcgct ccccgcgccg
      841 atcgacattg gcctgctcct cgtgctcggc acgttgtgca ccgggctttc gcacacgctt
      901 ttcatcgcga gcatgcgtcg cgtatccgcg catacggcga gcgtggtcgc cgcgctcgag
      961 ccggtgtacg gtatcgtgct cgcggcgctt ctgctcggcg agcggccggg ttcgcggacg
     1021 atcatgggcg gcgtgctgat cgttgccgcc gctctcaatg cgtcgcttcg caccgaagtt
     1081 ggcgaggcgc gcggcgttta ggtagcatca tacgagcgaa gccttgcaca tgactcaatt
     1141 ggacattggt ctcgtcgggc tgggcaggat gggcggcaac atggcccggc ggctcgcgcg
     1201 cggcggtgtg cgcgtgatcg gcttcgaccc ggatgaacgc gtccgccgct cgctcgcaga
     1261 cgacatcgtc gaggcgcgct cgctccatga cgtcgtcgac gcgttgactg cgccgaggat
     1321 cgtctggctg atggtgcctg ccggcgatgt gacgcagcgc accgtcgacg aactcgcggc
     1381 gctccttgca ccgggtgacc ttctcgtcga cggcggcaac gcgaactacc acgactcgca
     1441 gcgacgcggc ctcgcgctcg cggcgcgcgg cattcgcttc gtcgattgcg gcgtatcggg
     1501 cggcgtgcat ggcctcgcga acggctacgc gctgatgttc ggcggcgatg agaaggcagc
     1561 ggcgatgctc ttgccctacg tgcgcattct cgcgcccgcg gccgatgcgg gctggctgca
     1621 ttgcggcccc gtcggcgccg gtcacttcgt caagatgatc cacaacggca tcgaatacgg
     1681 catgatgcag gcgctcgccg aaggcttcgc gctgatgcac aagcggaccg atctcgcgct
     1741 cgacgtcgcg aagattgcgc aaatgtggcg tcacggcagc gtcgtgcgct cgtggctgct
     1801 cgatttgacc gccgagtttc tcgcccagga tgccgagctt cacgacatcg caccgatcgt
     1861 cgccgattcc ggcgaaggcc gctggaccgt gcaggaggcg atcgagcagg gcgtgccggc
     1921 gccggtcctg tcgctcgcgc tgatggcgcg cttttcgagc cagggcggcg gcgactacgc
     1981 gaatcgaatg ctcgcgatga tgcgcaaggg cttcggcgga cacgccgtcg ccagtgcaga
     2041 cgacaagcga tgaagggcag gtcgagggca tgatcgtcgt gatcatgggc gtttcacgct
     2101 cgggccgatc gacggtgggc gctgcgctcg cgcgcgatct gcgcttcgtg cacctgcgcg
     2161 gcgacgccga aacgatcgcg gcacgcctcg cgacccggca gcatcggtgc atgccggcga
     2221 cat
//
LOCUS       DASUJG010000293         3358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_696545, whole genome shotgun sequence.
ACCESSION   DASUJG010000293 DASUJG010000000
VERSION     DASUJG010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696545"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="VFX38_06305"
     CDS             <1..838
                     /locus_tag="VFX38_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314500.1"
                     /translation="LPPDAANAHDGVIAIRVWMRPGLGGRGPDAGGIHIPPTLGTTEA
                     IGAQYQREWLQTFYGYIVEVVEPLAFVLLALLAWCFRGAISRGRFVPWLCAALLLTAA
                     YRLNQAVYAWMPYESLFMYGFVHLVLIPLGLAAWVMTWRHWYQLERWRWLTYAIGIIT
                     VLSVATVLSVVLGLKLPQGMFGMLRHIWRTPLAIVLVATAIVGLRKRQPDRVLAFIVV
                     MLVGASQFGWLETLGVPGIWFPFGVGVSLTQYLYAGIIVGLAALLIRSVQRVTANRLV
                     FA"
     gene            complement(942..2279)
                     /locus_tag="VFX38_06310"
     CDS             complement(942..2279)
                     /locus_tag="VFX38_06310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5314501.1"
                     /translation="MRVLPRSLFGRLLLVLAVGLTAALIGSATINLHEQHHLMEHAGV
                     ERIAEHIAYSTHLFEQLTPADRRAVARHMSHWSWRYRIGNSPPSLAADRHLPGRLAAL
                     APALASRLGAAYPIRVAGPDPAGRARDGRSRDAGEGFVAAVGLHDGDWLTISTRFRPP
                     PPDWPVSLFVKLAILLLVALLLSFLAVTWVTRPLAMLAEAARALGRDIRRPPLVETGP
                     SEVRRAAAAFNLMQQRLLGFLDGRMRTFLAVSHDLKTPVTRLRLRAEMLEDPGLREAF
                     NEDLDEMDAMLSATLAYLRGESEQEPAQPLDVNALLASMASDAEALGQPVTVAGRAAA
                     PYRARPRALRRCLDNLVQNALRYGGSAEIAIEDENAALVIRVSDRGPGLPEAELERVF
                     EPFYRGEASRNRATGGTGLGLASAREAATAHGGTLTLANREGGGLVATLTLPR"
     gene            complement(2279..3001)
                     /locus_tag="VFX38_06315"
     CDS             complement(2279..3001)
                     /locus_tag="VFX38_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5314502.1"
                     /translation="MSDGEHILVVDDDGGIRRLLADYLGRNGYRVSTAADGAQMQRVL
                     GSAEPDLIVLDIMLPGADGIELCRRIRAGSEVPMIMLTARGEETDRVVGLEIGADDYL
                     AKPFSPRELLARIKSVLRRTRALPPNLRRAPRAACYRFSGWTLDPATRQLTAPDGVLV
                     PLSGAEYLLLIALLEHPGVVLSRDQLLDLTRGRDAAPFDRSIDVQVSRLRQKLREDAR
                     EPTIIRTLRSEGYVLATRVDNA"
     gene            3048..>3358
                     /locus_tag="VFX38_06320"
     CDS             3048..>3358
                     /locus_tag="VFX38_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314503.1"
                     /translation="MRRPRAGSGQRSERPLYADRGFAARDFVTDCCHPRHRKRGLQKS
                     ARPTHPVNISPVGWVSTERFRRHPEIPNWSRNMFKQALIVPFAVLALAAGTALAHPSA
                     GS"
BASE COUNT          534 a         1056 c         1180 g          588 t
ORIGIN      
        1 cttgccgccg gacgccgcga acgcgcacga cggcgtcatc gcgatacggg tctggatgcg
       61 tcccggactg ggggggcgcg ggccggatgc gggcggcatc catattcccc ccacgctcgg
      121 aacgacggaa gcgatcggcg cccagtacca gcgggaatgg ctgcaaacat tctatggtta
      181 cattgtcgag gtcgtagaac ccctggcgtt cgtgttgctg gcgttgctgg cctggtgttt
      241 ccgcggggcg atttcgcgcg gacgctttgt gccctggttg tgcgcggcat tgctgctgac
      301 cgccgcgtac cggctcaatc aggcggttta tgcgtggatg ccgtacgaaa gtttgtttat
      361 gtacggcttt gtgcatctcg tcctgatacc actggggttg gcggcatggg tcatgacatg
      421 gcgacattgg taccagttgg aacgctggcg atggctgacc tacgccatcg gcatcattac
      481 cgtgttgagc gttgcgaccg tgctgagcgt cgtgctgggg ttgaaactgc ctcagggcat
      541 gttcggcatg ctgcgtcaca tttggcgtac cccgctggcc atcgtgctgg tcgccaccgc
      601 catcgtcgga ttgcgcaaac ggcagccgga tcgtgtgctc gcgttcatcg tcgtgatgct
      661 ggttggggcc agtcaattcg gctggctgga aaccttgggt gtgcccggca tctggtttcc
      721 gtttggcgtc ggcgtctcgc tgacgcagta cctgtacgcg gggatcatcg tcggtctggc
      781 ggccttgttg attcgaagcg tgcagcgggt aacggcgaat cgactggttt tcgcctgatt
      841 gatcccgtct ctcggccggg aaacgccggc gtcacgcaaa gcccgctctg gccacgggcg
      901 aggagcgaca cggcatgcac aaggcggggc agcgcgccga ttcagcgggg caaagtgagg
      961 gtggcgacga gcccgccgcc ctcgcgattt gcgagcgtga gcgtgccacc gtgggcggtg
     1021 gcggcctctc gcgcgctcgc gagtccgagc ccggtgccgc cggtcgcgcg attgcgcgaa
     1081 gcctcgccgc gatagaaggg ttcgaacacg cgttcgagtt cggcctcggg aagtcccggt
     1141 ccgcggtcgc tcacccggat gacgagcgcc gcgttctcgt cctcgatcgc gatttccgca
     1201 ctgccgccgt agcgcagcgc gttctgtacc agattgtcga gacagcggcg caacgcccgc
     1261 ggacgcgccc gatacggcgc cgccgcccgg cccgcgaccg tgaccggctg gccgagggct
     1321 tcggcatcgg aggccatgct cgcaaggagc gcattcacgt cgagcggctg cgccggctcc
     1381 tgttcgcttt cgccgcgcag ataggcgagc gtcgcggaaa gcatcgcgtc catctcgtcg
     1441 agatcttcgt tgaaggcctc gcgcaggccg gggtcttcca gcatttccgc gcgcaggcgc
     1501 aggcgcgtga ccggcgtttt cagatcgtgc gagaccgcga gaaaggtgcg catgcgcccg
     1561 tcgagaaatc cgagcaatcg ctgttgcatc aggttgaagg ctgcggcggc ccgccgcact
     1621 tcgctcggac cggtttcgac gagcggcggg cggcggatat cgcgcccgag cgcgcgggcc
     1681 gcctcggcga gcatcgcgag cggccgggtg acccaggtca cggcgagaaa cgaaaggagc
     1741 aaggcgacga gcagcaggat cgcgagtttg acgaagagcg acaccggcca gtcgggcggc
     1801 ggcgggcgga aacgggtgga aatcgtgagc cagtcgccgt cgtgcaatcc gacggcggcg
     1861 acgaagcctt cacccgcgtc gcgggatcgc ccgtcgcgcg cgcggccggc cggatccggt
     1921 cctgcgacgc ggatcggata cgccgctccg agccggcttg cgagtgccgg cgcaagggcg
     1981 gcgagccgcc cgggaaggtg gcgatcggcg gcgagactcg gaggagagtt tccgatgcga
     2041 tagcgccaac tccaatggct catgtgccgc gccaccgcgc ggcgatccgc gggcgtgagc
     2101 tgctcgaaca gatgcgtcga ataggcgatg tgttcggcga tgcgttcgac tcccgcatgt
     2161 tccatcagat ggtgctgttc gtgcagattg atggtggccg agccgatcag ggctgcggtc
     2221 aggccgacgg cgagcacgag cagcagccgg ccgaagagcg aacgcggcag cacgcgcatc
     2281 aggcgttgtc cacccgcgtg gcgagtacat aaccttcgct gcgcagggtg cggatgatgg
     2341 tgggctcgcg ggcatcctcg cgcagcttct ggcgcagtct gctcacctgc acgtcgatgc
     2401 tgcgatcgaa gggcgcggcg tcgcgtccgc gggtaagatc gagaagttga tcgcgcgaca
     2461 gcacgacgcc cggatgttcg agcagcgcga tcagcagcag atattcggct ccgctcaggg
     2521 gaaccagcac tccgtccggg gcggtcaatt ggcgggtcgc gggatcgaga gtccagccgg
     2581 aaaaccgata acatgcggcc cgcggagcgc gccgcagatt cggcggcagg gcccgggtgc
     2641 ggcgcagcac gctcttgatg cgtgcgagaa gttcgcgcgg actgaagggc ttcgcgaggt
     2701 agtcgtcggc accgatttcc agtccgacga cgcggtcggt ttcctcgccg cgcgcggtga
     2761 gcatgatcat cggcacctcg ctcccggcgc ggatgcgccg acagagttcg atgccgtcgg
     2821 cgccgggcaa catgatgtcg agcacgatca gatcgggctc ggcggagccg agtacgcgct
     2881 gcatctgcgc gccgtcggcg gcggtggaaa cacggtagcc gttgcgtccg agatagtccg
     2941 cgagcaggcg gcggattcct ccgtcgtcgt ccaccacgag aatgtgctcg ccgtcgctca
     3001 tgcgtacctc gggaagcgaa tcgatccctc ctggacgcga aggcccgttg cggcgacctc
     3061 gcgccggatc cgggcagcgc tccgagcgtc ctttatacgc cgatcgcggc ttcgccgccc
     3121 gcgattttgt aacggattgt tgccacccgc gccatcgcaa acgaggctta caaaaatctg
     3181 cccgcccgac acatccggtc aacatttctc ccgtaggatg ggtctcgacg gaaagattcc
     3241 gtcgtcaccc ggaaatcccg aactggagtc gcaacatgtt caagcaagca ctgatcgttc
     3301 ccttcgccgt gctggcgctc gcggccggca cggctctggc ccatccatcg gcgggttc
//
LOCUS       DASUJG010000294         6659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_573806, whole genome shotgun sequence.
ACCESSION   DASUJG010000294 DASUJG010000000
VERSION     DASUJG010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6659
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573806"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..135
                     /locus_tag="VFX38_06325"
     CDS             <1..135
                     /locus_tag="VFX38_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011257047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX5314504.1"
                     /translation="ARSPLKAGEKLADRVHAKLSSIARQPALIRSFFRHPSVAYISDF
                     "
     gene            complement(495..2402)
                     /locus_tag="VFX38_06330"
     CDS             complement(495..2402)
                     /locus_tag="VFX38_06330"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HEX5314505.1"
                     /translation="MRHSAMARFMNETNRRHNRKLEDYDGLWRWSVEHREEFWPLVWN
                     FFDVQASRRWDTVLKNDVMPGAKWFEGARLNFAENLLRYRDDRTALVFRNEAGHRRTL
                     SYAELYREVARLRAGLDAAGIVAGDRIAAYLPNMPETVVAMLAAASVGAVFSSTSPDF
                     GADGVLDRFGQIEPKILFVADGYFYNGKTHDGLARAREIAAHLPSVARIVVVPHVSEK
                     PDLAGIPNAVPYQDFLGEADEIEFVQLPFDHPQFILYSSGTTGKPKCIVHGVGGVLLQ
                     HLKELGLHTDVSRDDVVFYFTTCGWMMWNWLVSALALGAAVVLYDGSPMHRTADALLR
                     MAEEEKITVFGTSAKFISALEKAAVGTAAQKQLASVRTILSTGSPLMPENFDYVYQTL
                     KPDVQLASISGGTDIVSCFALGCPILPVWRGELQCRGLGLAVEIFDDDGNALTGATGE
                     LVCTRAFPVMPVSFWNDPDGARYRAAYFERFPGIWHHGDWAKVTEHGGLVIYGRSDAV
                     LNPGGVRIGTAEIYRQVEKLPEIMESLAIGQQWHGDVRIVLFVRMQPGKALDDALAHR
                     IRQVIRKNETPRHVPAVILEVPDLPRTRSGKISEIAARDVVHGRKVKNTEALANPEAL
                     EAFRNRPELAG"
     gene            2374..2499
                     /locus_tag="VFX38_06335"
     CDS             2374..2499
                     /locus_tag="VFX38_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314506.1"
                     /translation="MNRAIAEWRIVSGAGAHMGWSLLIVSGPAIYTFIPAQAGIQ"
     gene            complement(2879..3820)
                     /locus_tag="VFX38_06340"
     CDS             complement(2879..3820)
                     /locus_tag="VFX38_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012509978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX5314507.1"
                     /translation="MNRILVIAQHDGKALSSATARAVSCAQKVGGDIDIAVFAKDAAS
                     IAAEAAKLDGVAKVLKIERAENAAPLAAVLAPQIAALAKDYSQVLMASTTFGKDVLPR
                     AAALAGVAMVTDVMEVIGAREFKRPIYAGNVIVTVTVPGDGPVFATARAASWKAAGSG
                     GNAAIEARTLDVTLPTHTEYLKLESGESGRPDLQSAARVVSGGRGVGSEENFKIIYSL
                     ADKLGAAVGASRAAVDAGFVPNDMQVGQTGKIIAPELYIAIGISGAIQHLTGIKDAGT
                     IVAINKDADAPIFEIADLGLVGDLFKLLPELEAELAK"
     gene            complement(3817..4566)
                     /locus_tag="VFX38_06345"
     CDS             complement(3817..4566)
                     /locus_tag="VFX38_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX5314508.1"
                     /translation="MKILVAIKRVVDFNVRVQIRRDGAGVETQGVKMGINPFDEIALE
                     EALRIREGGTEAEIIAVSIGPDAAQQQLRTALAMGADRALLVKTDEAVQPPLAARALL
                     KIAEEEKPDLVLLGKQAIDDDANQTGQMLAALWDRPQACFTSKLELADGKATATREVD
                     EGLEVIRVQLPAVVTTDLRLNTPRFVKMPDIMKAKKKPLDVRDLAALGIEDAPALATG
                     AFEAPPKRQGGRKVESVAELVAELKDRGLVK"
     gene            complement(4653..5156)
                     /locus_tag="VFX38_06350"
     CDS             complement(4653..5156)
                     /locus_tag="VFX38_06350"
                     /inference="COORDINATES: protein motif:HMM:NF025675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HEX5314509.1"
                     /translation="MTTIQLTERDLVDAYLLAKRWGKGMWSMYAASTAVYVGLGLWLL
                     TFPRVLRPFGIAALVFAAVSWILAAAGRYVLIPIVAKRRFRASKALARPFRLDWNEDG
                     LEIESAIGIARPPWSDFRKWRENGRLFLVYSPTGVARILPKASFPNRSGAEEFRSLLE
                     RKLGPSR"
     gene            complement(5242..6279)
                     /locus_tag="VFX38_06355"
     CDS             complement(5242..6279)
                     /locus_tag="VFX38_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314510.1"
                     /translation="MNDRDSNTPLALMVGLPDDMMVNVWPRSRNGDLDVSVPGNARLA
                     DDLPQREQQRLARTYFGPDIPIQLPAAVRQNAVVNAIADPDVSSASLRVLEAFLQDGA
                     CACFNHPTAVLECGRERVAEKLSAVPGMLVPRTIRRKIGEPADLLREAAENGIGYPLI
                     VRIAGSHRGATTVKIDRPEDAAPALRAIPWGGRELYVTEYVDYRDDDGLFRKMRIIFV
                     GKQRILRHFVIAGDWQVHRGDRLMQHVNEEATMLCNFDSTLLPQLRGTLESIAEAMDL
                     DFFGIDCKLRPDGSLLMFETNAMMNTMYNDAPSPNCWDAPVRRIREAVGALLFEPARW
                     RHPPRRRSIAQ"
     gene            complement(6316..>6659)
                     /locus_tag="VFX38_06360"
     CDS             complement(6316..>6659)
                     /locus_tag="VFX38_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017917116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX5314511.1"
                     /translation="LPEYDYLTRSLPPRDRLSSVFFSATSHDENQPVHLKIPDLDICY
                     ERCTREFGNPCTRFCPAAVYEMVEEDGERRLRINAANCVHCKTCDIKDPYENILWTTP
                     EGGSGPNYQNL"
BASE COUNT         1150 a         2031 c         2199 g         1279 t
ORIGIN      
        1 gcccgcagtc cactcaaggc cggcgagaag ctcgccgatc gcgtgcacgc gaaactctcc
       61 tcgatcgctc gccaacccgc cttgattcgt tccttcttca ggcatccaag tgttgcctat
      121 atttctgact tctgagtaaa tacgcgaatg agctttcgct caggaagcgt ttgcaacagt
      181 tggtggcccc atttgccgac ctttacggag gcgaggatga gcggaaggca ttcatagcca
      241 agtgtgtcgc cagtcgcaat tatctgactc actacgacaa aagcatcgaa aaagaggcgt
      301 tgggccttcg cgagttgtgg gtgctatgtc aaaagctgga ggcactttgt cagatgcatc
      361 tcctttcgga aattggattt gacaaggaat ctgtagtgac aatggcaagg caacacggcg
      421 cgctaaagcg tcggctatcc ggctggggtt aggagtttga ggacgtagta aattgtgaaa
      481 ccgcgtcacg acaactagcc ggccagttca gggcggttgc ggaaggcttc gagggcttcg
      541 gggttggcga gggcttcggt gtttttgacc ttgcggccgt ggacgacgtc gcgggctgcg
      601 atttcgctga tctttccgct tcgcgtgcgc gggagatcgg gcacttcgag gatcacggcg
      661 ggcacgtggc gcggcgtttc gttcttgcgg atgacctggc ggatgcggtg ggcgagcgcg
      721 tcgtcgagcg ctttccccgg ctgcatgcgt acgaagagca cgatgcggac atcaccgtgc
      781 cattgctggc cgatcgcgag gctttccatg atttccggca gcttttcgac ctgacggtag
      841 atctcggctg tgccgatgcg cacgccgccg gggttgagca cggcgtccga gcggccgtag
      901 atgacgagcc cgccgtgttc ggtgactttt gcccagtcgc cgtgatgcca gatgccgggg
      961 aagcgttcga agtaggcggc gcgataacgc gcaccgtcgg gatcgttcca gaaggaaacc
     1021 ggcatgaccg gaaacgccct ggtgcagacc agttcacccg tggcgccggt gagcgcgttg
     1081 ccgtcgtcgt cgaagatttc gacggcgagg ccgagaccgc ggcattgcag ttcgccgcgc
     1141 cacacgggga gaatcgggca gccgagcgcg aagcaggaaa caatgtcggt tccgcccgag
     1201 atcgaggcga gttgcacgtc gggctttaat gtctgataaa cataatcgaa gttctccggc
     1261 atgagcggcg agccggtaga aagaatcgta cgcacgctcg caagctgctt ttgcgccgcg
     1321 gtgccgacgg cggccttttc cagcgcggag atgaacttgg cgctggtgcc gaaaacggtg
     1381 atcttttctt cttcggccat gcgcagcaaa gcgtccgccg tgcgatgcat cggagagccg
     1441 tcgtaaagca cgaccgccgc gccgagcgcg agcgcggaaa ccagccagtt ccacatcatc
     1501 cagccgcagg tggtgaagta gaagacgaca tcgtcgcgcg aaacgtcggt gtgcagaccg
     1561 agttctttca gatgctgcag cagcacgccg cccacgccgt gcacgatgca cttgggtttt
     1621 ccggtggtgc cggaagaata aagaatgaac tgcggatgat cgaagggcag ttgcacgaat
     1681 tcgatttcat ctgcttcgcc gagaaaatcc tggtaaggta cggcgttcgg aattccggcg
     1741 agatcgggtt tctcgcttac gtgcggaacg acgacgatcc gcgcgaccga cggcaggtgc
     1801 gccgcgattt cccgcgcgcg cgcaaggccg tcgtgcgtct tgccgttgta aaagtagccg
     1861 tcggccacga acagaatctt cggttcgatc tggccgaagc ggtcgagcac gccgtcggcg
     1921 ccgaagtcgg gcgaggtgga cgaaaagacg gcgccgacgc tcgccgccgc gagcatggcg
     1981 acgaccgttt cgggcatgtt cggaagatag gccgcgatgc ggtcaccggc gacgatgccg
     2041 gcggcgtcaa gccccgcgcg caggcgtgcc acttcgcggt agagttcggc gtagctcagc
     2101 gtgcggcgat ggccggcttc gttgcggaag acgagcgcgg tgcggtcgtc gcggtagcgg
     2161 agcaggtttt cggcgaagtt gagtcgcgcg ccttcgaacc atttcgcccc gggcatgaca
     2221 tcgttcttga gcaccgtgtc ccagcggcgt gaagcctgga cgtcgaagaa attccacacc
     2281 agcggccaga attcttcccg atgctcgacc gaccagcgcc agagtccgtc gtaatcttcg
     2341 agtttgcgat tatggcggcg attcgtttca ttcatgaacc gcgccatcgc cgaatggcgc
     2401 atcgtttccg gtgccggagc ccacatgggt tggtcgttgc tcatcgtctc cggtcccgcg
     2461 atttacacct tcattcccgc gcaggcggga atccagtaga agaagaattc cgccccgtgg
     2521 tcggaatgca aagtccgact ggaccccggc cttcgccggg gtgacgagaa ggaaggtcgc
     2581 aaagtcccag gccgggataa gcgcgcgtcg cgtgcgtttc cggcaatccg cgtcaaattc
     2641 cggcaacgac cttcggtctt atgccggcct acgaatttgt ggctcattgc tgtttgccgg
     2701 cgggatgccg gcgctcccag cccgatgttc atttggcgcg tatcgtaggc ccgcataagc
     2761 gcagcgttgc gggcattcgg cgccggacgc cggcaacgac cttcggcctt atgccggcct
     2821 acaaatttgg ctctcgttgc tgtttgccgg cgggacgccg gcgctcccgg ggtgacgctc
     2881 acttcgcgag ttcggcttcg agttcgggaa gcagcttgaa cagatcgccg acgaggccga
     2941 gatcggcgat ctcgaagatc ggcgcgtcgg cgtccttgtt gatggcgaca atggtgcccg
     3001 cgtccttgat gccggtcaga tgctggatcg cgccggagat tccgatcgcg atataaagct
     3061 cgggcgcgat gatcttgccc gtctgcccga cctgcatgtc gttcggcacg aagcccgcgt
     3121 ccaccgccgc acgcgaggca ccgacggcgg cgccgagttt gtcggcgagc gagtagatga
     3181 tcttgaagtt ctcctcgctg ccgactccgc gtccgccgga gacgacgcgt gcggccgatt
     3241 gcaggtcggg gcgcccgctt tccccggatt cgagcttcag gtattcggta tgggtcggca
     3301 aggtcacgtc gagcgtgcgc gcttcgatcg cggcattgcc gccgctgccg gccgctttcc
     3361 aggaagcggc gcgtgcggtc gcgaacacgg gaccgtcgcc cggcacggtg acggtgacga
     3421 tcacgttgcc ggcgtagatc gggcgtttga attcgcgggc accgatcact tccatcacat
     3481 ccgtcaccat cgcgacgccc gcgagtgcgg cggcgcgcgg cagcacgtcc ttgccgaacg
     3541 tggtagaggc catgagcacc tgcgagtagt ccttcgcgag cgccgcgatc tgcggggcga
     3601 gcacggccgc cagcggcgcc gcgttctccg cacgttcgat cttcagcacc ttcgccacgc
     3661 catcgagctt tgcggcttcg gcggcgatgg aagcggcgtc ttttgcaaac acggcgatgt
     3721 caatatcgcc gccgaccttc tgcgcgcagg agacggcgcg cgcggtcgcg gaggagagtg
     3781 ctttgccgtc gtgttgcgcg atgacgagaa tgcggttcat ttcacgagcc cccggtcctt
     3841 gagttcggcc acgagttcgg cgaccgactc gaccttgcgc ccgccctgcc gtttcggcgg
     3901 cgcttcaaag gcaccggttg cgagcgccgg cgcgtcttcg atgccgagcg ccgcgagatc
     3961 gcgcacgtcg agcggcttct tttttgcctt catgatgtcc ggcatcttga cgaagcgcgg
     4021 cgtgttgaga cgcagatccg tggtgaccac ggccggcagt tgcacgcgga tgacttcgag
     4081 tccttcgtcc acctcgcggg tcgccgtcgc cttgccgtcg gcgagttcga gcttcgaggt
     4141 gaaacaggcc tgcggacgat cccagagcgc ggccagcatc tgcccggtct gattcgcatc
     4201 gtcgtcgatc gcctgcttgc cgagcagcac gagatcgggc ttttcttcct cggcgatttt
     4261 caacagcgcg cgggcggcga gcggcggttg cacggcctcg tcggtcttga cgagcagggc
     4321 gcggtccgcg cccatcgcga gcgcagtgcg caactgttgc tgagcggcgt cgggtccgat
     4381 cgagaccgca atgatttccg cctccgttcc gccttcgcgg atgcgcagcg cctcttccag
     4441 cgcgatctcg tcgaaggggt tgattcccat cttcacgccc tgggtttcga cgcccgcgcc
     4501 gtcgcgccgg atctgcacgc gaacgttgaa gtccaccacc cgtttgattg caaccagtat
     4561 tttcatgagc ccttcgccgt tttctccatc ggcgacaatt ctaccggaag cctgatcccg
     4621 gccgcgacgc gccgccgcgg cgggcgcgac tctcagcgcg acggcccgag tttccgttcc
     4681 agcagactgc ggaattcttc cgcgccgctg cggttcggaa acgacgcctt ggggaggatg
     4741 cgcgcgaccc cggtggggga atagacgagg aacagacggc cgttctcgcg ccatttgcgg
     4801 aaatccgacc acggaggtcg ggcgatcccg atcgcactct cgatctcgag gccgtcttca
     4861 ttccaatcga ggcggaaggg ccgcgcgagc gccttgcttg cccgaaagcg ccgtttcgcg
     4921 acgatcggga tcaggacata gcgtccggca gcggcgagaa tccatgacac cgcggcgaag
     4981 acgagcgcgg cgatgccgaa ggggcggagc acgcgaggga atgtgagcaa ccacaggccg
     5041 agtccgacat aaacggcggt gctagccgcg tacatggacc acattccctt cccccaacgc
     5101 ttggcgagca gataggcgtc cacgagatcc cgctcggtca attgtatggt ggtcacggtc
     5161 gaattccagc aggccgatgc cgggagcggt gattgcggat tcgcagcctc gcgattgccc
     5221 cgccgcccgt ggggatcggc gtcattgcgc gatggaacgg cggcggggag gatgacgcca
     5281 cctggcgggt tcgaacagca gcgcgcccac ggcttcgcgg atgcgccgca ccggtgcatc
     5341 ccagcagttg ggcgagggcg catcgttgta catggtgttc atcatcgcgt tcgtttcaaa
     5401 catcaggaga ctgccgtccg gacgcagctt gcagtcgatg ccgaaaaagt cgaggtccat
     5461 cgcctcggcg atgctttcca gcgtgccgcg aagttgtggc agcaatgtgg agtcgaagtt
     5521 gcacagcatc gtcgcttcct cgtttacgtg ctgcatgagc cggtcgccgc ggtgtacctg
     5581 ccagtctccc gcgatcacga agtggcggag aatccgctgc ttgcctacga agatgatccg
     5641 catcttgcgg aacagtccgt cgtcgtcgcg atagtccaca tactccgtca cgtacagctc
     5701 gcgcccgccc cagggaatgg cgcgaagcgc cggcgcggca tcctcggggc gatcgatttt
     5761 gacggtcgtc gcgccacgat gcgatccggc gatgcgcacg atcagcggat agccgatccc
     5821 gttttccgcc gcctcgcgca ggaggtcggc gggctcgccg atcttgcgcc ggatcgtacg
     5881 cggcacgagc atgccgggga ctgccgagag cttttccgca acgcgttcgc gcccgcattc
     5941 caggaccgca gtcggatgat tgaagcaggc gcaagcgccg tcctgaagaa atgcctcgag
     6001 cacccgcagg gaagcgctcg atacgtccgg gtccgcgatc gcgttgacga ccgcgttctg
     6061 ccgtaccgcc gcaggcaatt ggatgggaat gtcggggccg aagtaggttc gcgcgagccg
     6121 ctgctgttca cgttgcggca agtcgtcggc gaggcgggca tttccgggca cgctcacgtc
     6181 gagatcgccg ttgcggctgc gcggccagac gttgaccatc atgtcgtcgg gcaggccgac
     6241 catcagcgca agcggcgtgt tcgagtcccg atcgttcatc tgccggaaac ctcagctcga
     6301 tgtcgcgcga cgttcttaca aattctggta attcgggccg gagccgccct cgggcgtggt
     6361 ccagaggatg ttttcgtagg gatccttgat gtcgcaggtc ttgcagtgca cgcagttggc
     6421 ggcgttgatc cgcaggcgcc gctcgccgtc ttcctcgacc atttcgtaca ccgccgcggg
     6481 acagaagcgg gtgcaggggt tgccaaactc ccgtgtacag cgttcgtagc agatatcgag
     6541 atcggggatt ttgagatgca ccggctggtt ttcgtcgtgc gaagtggccg agaagaagac
     6601 ggacgacagg cgatcgcgag gcggcaggct gcgcgtcagg tagtcgtact ccggaagcc
//
LOCUS       DASUJG010000295         4494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_574057, whole genome shotgun sequence.
ACCESSION   DASUJG010000295 DASUJG010000000
VERSION     DASUJG010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574057"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VFX38_06365"
     CDS             complement(<1..256)
                     /locus_tag="VFX38_06365"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258373.1"
                     /note="Converts isocitrate to alpha ketoglutarate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEX5314512.1"
                     /translation="MPYRHIEVPAGGEPIAIHPDGSLDVPDRPIVVCIRGDGIGADVT
                     PVMTSVVEAAVERAYGARRHIAWLRAWAGEEAAALYDGACL"
     gene            complement(384..3179)
                     /gene="treY"
                     /locus_tag="VFX38_06370"
     CDS             complement(384..3179)
                     /gene="treY"
                     /locus_tag="VFX38_06370"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEX5314513.1"
                     /translation="MTAPLATYRLQFHRDFRFADAERIVPYLADLGVSHLYASPWLMA
                     RAGSRHGYDITDHNAFNPEIGTAEDFERLSALLRRHRLGHILDFVPNHMGIGDDNAWW
                     LDVLEWGRASPFAACFDIDWSGTEPGLRNKLLLPFLGDHYGRVLECGELELVFDAEQG
                     SFAVRYFEHRFPLAPRSYANVLRAAHTTLPAALAALAGEFAALRLRAGGASGRAAETR
                     QRAAERKRQLADLARRDSEARAAIAQAVATFGGQAGDARSFRPLHRLLEAQFYRLAYW
                     RAASDDINYRRFFDNNDLAALRIEDPQLFATAHRLVAQLLADGRLDGLRIDHIDGLHD
                     PAEYCRRLRELVQRPFFVVVEKILAHHEALRAWPVAGTTGYDFLADVTALLVASASEA
                     AMSRTYRRFLGRDPDFGDIVYRAKREIMETRLAGELHGLARDLKRIAAADWNARDYSR
                     ESLERALEEVVACFPVYRSYVDGRGADEIDCRYIDWAVAQARQRSREPDGSIFDFIRA
                     ALTAELAPVPGARRRAVLRFAMRFQQYTAPVMAKGFEDTAFYRYHRLICLNDVGENPL
                     RFGLSVAAFHHANAERARLHPHGLLATATHDSKRGEDVRARLAVLAAMPAAWDRQVRR
                     WAHLNRRKKRGVRDRAAPDRNDEYFLYQTLVGAWPAELLGEDSDEATVGDFLARVQAC
                     FVKSIREAKLHSSWIDPDVAYEEAASDFVARILDPERNRPFFDDCRSFARELAALGVD
                     ASLVQLVLKLTSPGVPDMYQGGELWDLNLVDPDNRRSVDFGLRERRLREIGEVARLAA
                     EQRIAALRKLRAHWEDGKIKLFVLHALLDFRRRHPELFSDGSYVPLAVEGPQAARICA
                     FERSCGDDAIVVAVAVPAGEHGGDWTETVVELAGEKRGAWQELLSGRSFSGSQFRAAT
                     LFANLPIAVLASARGHS"
     gene            complement(3176..>4494)
                     /gene="malQ"
                     /locus_tag="VFX38_06375"
     CDS             complement(3176..>4494)
                     /gene="malQ"
                     /locus_tag="VFX38_06375"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX5314514.1"
                     /translation="PQVRYREVAELKFPMLEEAYRHFRRQHLAAENSLHARAFREFQR
                     ERGATLRRFATFHVLAESHDEFDWRSWPANERDPASETVASFVLEREERIEFHEYLQW
                     QCDGQLRAAAAKAHAAGMSIGLYRDLAVGADANGAETWSAQRTIVAGAAVGAPPDQLN
                     LDGQNWGFPPPDPRAWQAENYVSFRELLRANMRHAGAMRMDHVLGLVRLWWIPAGARA
                     RDGAYQRYPWSELLAVLAETSRQERCLVIGEDLGTVPQGLREAMRGNGILSCRLLYFE
                     RRGRNFAAPQEYPPEALVAAATHDLPTLAAWWHGDDIALRDRLGLWPDAGQRASECNA
                     RIEDREALLRALREQGIANEEDGEEAPIAAVHRFLARTPCRLLMVQLEDALGARTQLN
                     VPGTTDEYPNWRLRLPCELNEVLSHPRMRRIAAAMHEEGRSDRQPP"
BASE COUNT          753 a         1430 c         1571 g          740 t
ORIGIN      
        1 gaagacaggc tccatcgtag agggcggccg cttcttcgcc ggcccaggct cgcaaccagg
       61 cgatgtgtcg cctcgctccg taggcgcgct cgaccgcggc ttcgacgacg ctggtcatca
      121 ccggggtaac gtcggcgccg atgccgtcgc cgcggatgca aacgacgatc ggccgatccg
      181 gaacgtcgag acttccgtcg ggatggatcg cgatcggttc gccgccggcg gggacttcga
      241 tgtgtcgata cggcatggaa agcgctgcga ccggggaccg ctcattttat cgcaggggga
      301 acttgccgct cgcgcacgcg attgcatccg gcccgccggg aaagggatcg gcgcgaacgc
      361 ccgtcatgcg cgaacggcag gcttcaggaa tggcctcggg cagaggcaag aaccgcgatc
      421 ggaaggttcg cgaagagcgt tgcggcgcgg aattgcgagc cgctaaagga tcggcccgaa
      481 agcaattcct gccacgcgcc gcgtttttct cccgcaagct cgacgacggt ttccgtccag
      541 tcgccgccat gctcgccggc gggcacggcg acggcgacga cgatggcgtc gtcgccgcag
      601 ctgcgctcga aggcgcagat gcgcgcggcc tgcgggcctt ccaccgcgag cggaacgtag
      661 gaaccgtcgg agaagagttc gggatggcgg cggcggaagt cgaggagcgc gtgcagcacg
      721 aaaagtttga tctttccgtc ttcccagtgc gcgcgcaatt tccgcagcgc cgcgatgcgc
      781 tgctcggccg caagccttgc gacttcgccg atctctcgca gccggcgctc gcgcaggccg
      841 aagtccacgg atctgcgatt gtcggggtct accagattca gatcccacag ttcgccgccc
      901 tgatacatgt cggggacgcc cgggctggtg agtttgagca cgagctgcac caggcttgcg
      961 tccacgccga gcgcggcgag ttcacgcgcg aaggagcggc aatcgtcgaa aaacgggcgg
     1021 ttgcgctcgg gatcgagaat ccgtgcgacg aaatcgctcg ccgcctcctc ataggcgaca
     1081 tccggatcga tccaggagga atgcaatttc gcctcgcgaa tcgactttac gaagcaggcc
     1141 tggacgcggg cgaggaagtc gccgaccgtt gcctcgtcgc tgtcttcccc gagaagttcg
     1201 gcgggccagg cgcctaccag ggtttggtac aggaaatatt cgtcgttgcg atccggtgcc
     1261 gcccggtcgc ggacgccgcg tttcttccgc cggttcaaat gcgcccagcg ccgcacctga
     1321 cgatcccagg cggcgggcat cgcggcgagc accgcgagcc gggcgcgcac atcctcgccg
     1381 cgtttgctgt cgtgcgtcgc ggtggcgagc aggccgtggg gatgaaggcg cgcgcgctcc
     1441 gcattcgcgt gatggaaggc ggcgaccgaa agaccgaaac ggagtggatt ttcgccgacg
     1501 tcgttcagac agatcagacg atggtagcgg tagaacgccg tgtcctcgaa ccctttcgcc
     1561 atgacgggag cggtgtattg ctgaaagcgc atcgcaaagc gcagcacggc gcgccgcctc
     1621 gcgcccggca ccggtgcaag ttcggccgtc agtgccgcgc gaatgaaatc gaaaatcgag
     1681 ccgtccggct cccgcgaacg ttgccgtgcc tgcgccacgg cccagtcgat atagcggcag
     1741 tcgatctcgt cggcgccgcg cccatcgacg taacttcggt acacgggaaa gcaggcgacg
     1801 acttcctcga gcgcgcgctc gaggctttcg cggctgtagt cgcgggcgtt ccagtccgcg
     1861 gccgcgattc gcttgaggtc gcgggcgagg ccgtgaagtt cgcccgcgag tcgggtttcc
     1921 atgatctcgc gcttggcccg gtacacgatg tcgccgaagt ccggatcgcg gccgaggaag
     1981 cgccggtagg tccggctcat cgcggcctcg ctcgcggatg cgacaagcag ggctgtcacg
     2041 tcggcgagaa aatcgtaacc ggtggtgccg gcgaccggcc aggcgcgcag tgcttcgtga
     2101 tgcgcgagga tcttttcgac gaccacgaaa aacggccgct gcacgagttc gcgcaggcgc
     2161 cggcaatatt cggccggatc gtgcaggcca tcgatgtgat cgatgcgcag gccgtcgagc
     2221 cttccgtcgg caaggagttg cgcgaccagg cggtgggcag tcgcaaatag ttgcggatct
     2281 tcgatccgca gtgcggcgag gtcgttgttg tcgaagaagc ggcgatagtt gatgtcgtcg
     2341 gaggcggcac gccagtaggc gagccggtag aactgcgctt cgagcagacg atgaagcggc
     2401 cggaacgatc gggcgtctcc cgcctgcccg ccgaaggtgg cgacggcctg cgcaattgcg
     2461 gcgcgcgctt cggagtcgcg gcgggcgaga tcggcgagtt gtctcttccg ttccgccgcg
     2521 cgctgccgcg tttcggcggc gcggcccgaa gcgccgcccg cgcgcaggcg aagcgccgcg
     2581 aactctccgg cgagggccgc gagcgcggcc ggaagtgtcg tgtgcgcagc gcgcaggacg
     2641 ttcgcgtaac ttcggggcgc gagtgggaag cgatgttcga aatagcgaac ggcgaagctt
     2701 ccctgctccg cgtcgaagac gagctcgagt tcgccgcact cgagcacccg gccgtagtgg
     2761 tccccgagga agggaagaag aagcttgttg cgcaggcccg gctccgttcc cgaccagtcg
     2821 atgtcgaaac aggcggcgaa gggcgaggct cgtccccatt cgaggacatc gagccaccag
     2881 gcgttgtcgt cgccgatgcc catgtggttc gggacgaagt cgaggatgtg gccgagccga
     2941 tggcgccgca acaacgcgct caagcgctcg aagtcttcgg ccgtgccgat ttccggattg
     3001 aatgcgttgt ggtcggtgat gtcgtagccg tggcggctgc cggcgcgcgc catgagccag
     3061 ggcgaggcgt agagatggct gacgccgagg tcggcgagat acgggacgat gcgttcggcg
     3121 tccgcaaaac ggaagtcgcg atgaaactgg aggcgatagg tcgcgagtgg cgcggtcatg
     3181 gcggctgccg gtcgcttcgc ccttcctcgt gcatcgccgc ggcgatccgc cgcattcgcg
     3241 gatgggaaag cacttcgttc aattcgcagg gaaggcgcag tcgccagttc ggatactcgt
     3301 cggtggtgcc gggaacattg agttgcgtgc gcgctccgag cgcatcctcg agctgcacca
     3361 tgagcagacg gcagggcgtg cgggcgagaa agcgatgcac cgccgcgatc ggcgcctctt
     3421 cgccgtcctc ctcgtttgca attccttgtt cgcgaagcgc acgcaggagc gcctcgcgat
     3481 cttcgatgcg cgcattacat tcacttgcgc gttgcccggc atccggccac agacccaacc
     3541 ggtcgcgcag ggcgatgtcg tcgccgtgcc accaggcggc gagcgtcggc agatcgtggg
     3601 tcgcggcggc gacgagggcc tcgggcggat attcctgcgg cgcggcgaag tttctcccgc
     3661 ggcgttcgaa gtacagcaaa cggcaggaga ggatgccgtt gccccgcatc gcctcgcgca
     3721 ggccctgcgg caccgtgccg agatcttcgc cgatgaccag acagcgttcc tgccggctgg
     3781 tctcggccag taccgcgagc agttcactcc agggatagcg ttgataggcg ccgtcgcgag
     3841 cccgtgcgcc ggcgggaatc caccacagac gcaccagtcc cagcacgtga tccatgcgca
     3901 ttgcgccggc gtggcgcatg ttcgcgcgga gaagttcgcg gaaggaaacg tagttctccg
     3961 cctgccacgc acgcggatcc ggcggcggaa atccccagtt ctggccgtcg agattcaact
     4021 gatccggcgg cgcgcccacc gcggcgccgg cgacgatcgt gcgctgcgcg ctccaggtct
     4081 cggcgccgtt ggcgtcggcg cccacggcga ggtcgcgata cagcccgatg ctcatcccgg
     4141 ccgcgtgcgc cttcgcggcg gcggcgcgca actgcccgtc gcactgccat tggagatatt
     4201 cgtggaattc gatgcgctct tcgcgctcga ggacgaagga agcgacggtc tcactcgccg
     4261 gatcgcgctc gttcgcgggc caactccgcc aatcgaattc gtcgtggctt tcggcgagca
     4321 cgtgaaacgt ggcgaagcgg cgcaacgtcg caccgcgctc gcgctggaat tcgcgaaaag
     4381 ccctggcgtg cagggaattt tccgcggcga gatgctgcct gcggaaatgt cgatacgctt
     4441 cctccagcat cgggaacttg agttccgcga cttcgcgata acgcacctgc gggg
//
LOCUS       DASUJG010000296         2579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_818497, whole genome shotgun sequence.
ACCESSION   DASUJG010000296 DASUJG010000000
VERSION     DASUJG010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818497"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VFX38_06380"
     CDS             complement(<1..362)
                     /locus_tag="VFX38_06380"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX5314515.1"
                     /translation="MTAAPATIAVTRGAPLRGTIALPGDKSITQRALFCAALAEGDSR
                     IAGALDAADTRATAAALAALGAELDWSGDAVRVHGTGGRWRAPRAPLDLGNSATGLRL
                     LAGAIAGRGIAARLTGDAS"
     gene            complement(359..1516)
                     /gene="hisC"
                     /locus_tag="VFX38_06385"
     CDS             complement(359..1516)
                     /gene="hisC"
                     /locus_tag="VFX38_06385"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019558029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX5314516.1"
                     /translation="MDKDSGIRSARFDPLEFCVPGLRALKPYEPGKPISELERELGIS
                     DIVKLASNENPLGPSPRALAAMHHAIAEVALYPDGNAHALKQALAVHHGIAPERIVVG
                     SGSDHLIELVARAFLAAGRSAVVAQYGFAIYAIVARAAGAELRIAAARSPSDSREPYG
                     HDPQTLRDAVDEATRVVFLANPNNPTGTWLRRPELEALLGAIPPDTIVVLDEAYCDYV
                     APFEREYADSRALLDAYPNLVVLRTFSKAYGLAGIRAGYALADPMVADLLNRVRLSFN
                     PSSVAQAGAVAALADRDHLKRTVEHNHGELRRLAEGLRKLGLACIPSVCNFVAADVGR
                     DGRELFRALLGEGIITRPLDGYGLPRHLRFSVGLAEQNERLMGALGRILAQ"
     gene            complement(1509..>2579)
                     /gene="serC"
                     /locus_tag="VFX38_06390"
     CDS             complement(1509..>2579)
                     /gene="serC"
                     /locus_tag="VFX38_06390"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEX5314517.1"
                     /translation="FSSGPAVLPAPVMEQARAELRDYAGSGLSILETGHRTAAFADVV
                     TRCESDLRALLAVPDDYGVLFLQGGASLQFVMVALNLSRSGEHVAYADTGHWSGRAIV
                     AARELRAVDVVATGESGGYLEVPQPETWRAFPDAAYLHYTPNETIDGVEFDFIPEAGA
                     MPLVADMSSTILSRPIDVTRFGLIYAGAQKNLGPTGLTLVVLRRDVAARAGIEVPSVL
                     RYRAHLEAPGGIYNTPPTFTWYLIGLYLAWVRAEGGLEEMQRRSQLRSDIVYRAIDES
                     GGFYRNRVAARSRSRTNIPFGIADPALETNFLQEADAAGLKQLVGHRSRGAMRASFYN
                     AMPISGAEALAEFMREFARRNG"
BASE COUNT          453 a          838 c          909 g          379 t
ORIGIN      
        1 gaggcatcgc cggtgagtcg cgcggcgatt ccgcgccccg caatcgcccc cgcgagaaga
       61 cgcagcccgg tcgccgaatt gccgagatcg agcggagcgc ggggcgctcg ccagcgcccg
      121 cccgtaccat gcacacgaac cgcatcgccg ctccagtcca gctcggcgcc gagagcggcg
      181 agcgcggcgg ccgtcgcgcg ggtgtcggcg gcatcgaggg cgccggcgat gcgggagtcg
      241 ccctcggcga gcgccgcaca gaaaagagcg cgctgcgtga tggatttgtc gcccggaagc
      301 gcgatggtgc cgcgaagcgg cgccccgcgc gtgaccgcaa tcgtcgccgg agccgccgtc
      361 actgggcgag aatgcggccg agcgcgccca tcaggcgttc gttctgttcg gcgagtccca
      421 ccgagaaccg caagtgccgg ggaagaccgt agccgtcgag cggccgggtg atgattcctt
      481 cgccgaggag cgcgcgaaac agttcgcggc cgtcgcgccc cacgtccgcg gcgacgaaat
      541 tgcacacgga gggaatgcac gcgagcccca gcttccgcag tccctccgcg agtcgtcgga
      601 gttctccgtg attgtgttcg acggtgcgct tcaggtggtc acgatcggcg agcgcggcga
      661 ccgctccggc ctgggcgaca ctgctcggat tgaacgacag gcgcacgcgg ttgagcagat
      721 cggcaaccat cggatcggcg agcgcgtagc ccgcgcggat gccggcaagc ccgtaggcct
      781 tggagaaggt gcgaaggacc accaggttcg ggtacgcgtc gagtaacgct cgtgaatcgg
      841 cgtactcgcg ctcgaagggt gcgacataat cgcaataggc ttcatcgagc accacgatcg
      901 tatctggcgg aattgctccg agcagcgctt cgagttcggg ccgccgcagc caggtgccgg
      961 tgggattgtt cgggttcgcg agaaagacga cgcgcgtcgc ctcgtccacc gcatcgcgaa
     1021 gagtctgcgg gtcgtgaccg tagggctccc gcgaatcgga gggagagcga gcggctgcga
     1081 tgcgaagctc cgcccccgcc gcgcgcgcga caattgcata aattgcgaag ccgtattgcg
     1141 cgacgacggc cgagcggccg gcggcgagaa aggcgcgcgc caccagttcg atgagatgat
     1201 ccgagccgct gccgaccacg atccgctcgg gcgcgatccc gtgatggacg gcgagcgcct
     1261 gcttgagcgc gtgcgcattc ccgtcgggat agagcgcgac ctccgcgatc gcgtgatgca
     1321 tcgcggcgag cgcgcgcggg ctcgggccga gcggattttc gttggaggcg agcttgacga
     1381 tgtcggagat gccgagttcg cgctcgagct cggaaatcgg cttgccgggc tcgtagggct
     1441 tcagcgcccg caggcccggc acgcagaatt ccagcggatc gaatctcgcg ctccgaatcc
     1501 cggaatcctt atccattgcg gcgggcgaac tcgcgcatga attcggcaag cgcctcggcg
     1561 cccgagatcg gcatcgcgtt gtaaaacgag gcgcgcatcg cgccgcgact gcgatgcccg
     1621 acgagctgct tgagtcccgc cgcatccgct tcctgcagaa agttcgtttc cagcgcggga
     1681 tccgcgatgc cgaagggaat gttcgtgcgg gaacgcgagc gcgccgcgac gcggttgcgg
     1741 taaaagccgc ccgattcgtc gatggcacga tagacgatgt ccgagcgcag ctgcgaacgc
     1801 cgttgcattt cctcgagccc gccctcggcg cgcacccagg cgagataaag cccgatcaga
     1861 taccaggtga aggtcggcgg cgtgttgtag atgccgcccg gcgcctccag atgcgcgcga
     1921 taacgcagga cggagggcac ttcgatcccg gcgcgcgcgg cgacatcgcg ccgcaggacg
     1981 acgagcgtga ggccggtcgg gccgagattc ttctgcgcgc cggcgtagat caatccgaag
     2041 cgcgtaacgt caatcggtcg ggaaagaatc gtcgaggaca tgtcggcgac gagcggcatc
     2101 gcgccggctt cgggaatgaa gtcgaactcg acgccgtcga tcgtctcgtt cggcgtgtaa
     2161 tgcagatagg cggcatccgg gaaggcgcgc caggtttccg gctgtggcac ctcgaggtaa
     2221 ccgccggact cgcccgtcgc gacgacgtcc acggcgcgca gttcgcgcgc cgcgacaatg
     2281 gcgcgcccgg accagtgtcc ggtgtccgca taggcgacat gttcgccgga gcgcgagaga
     2341 ttgagtgcga ccatgacgaa ttgcaagctc gctccacctt gcaggaacag cacaccgtag
     2401 tcatcgggga cggcgagaag cgcacgcaga tcgctctcgc agcgtgtaac gacgtcggca
     2461 aacgcggccg tacgatggcc ggtttcgaga atggacagac cgcttcccgc gtagtcgcgc
     2521 aactccgccc gtgcctgctc catcaccggc gccggcagca cggccgggcc ggagctgaa
//
LOCUS       DASUJG010000297         6283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_209280, whole genome shotgun sequence.
ACCESSION   DASUJG010000297 DASUJG010000000
VERSION     DASUJG010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6283
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209280"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1793)
                     /locus_tag="VFX38_06395"
     CDS             complement(<1..1793)
                     /locus_tag="VFX38_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEX5314518.1"
                     /translation="MNHLHEAIADKVAVWRAEGYPHSHSPAISEILDYAWADDERREL
                     RYLRAAQFRALETYWYLRLVMGTARIPDLYRQLFDGLTARLEAMGVSAQLFAQADFNE
                     DALFRRIRTDDVFVRKHKLESLRETLTLDYPSYILALAMGAGKTILMGTIVATEFAMA
                     QEYSEGDFVQNALIFAPGKTIIESLREIADMPFERILPPRMYKTFAASLKLTFTRDGA
                     KELPIVPGSSWNVVVTNTEKIRIQKPTGRRNGGGLRLNLHPGDDPEAEIANLRLQAIA
                     SLPHLAVFSDEAHHTYGQKLLGQIKKVRRTVDYLGKNTNLIIVVNTTGTPYFERQPLK
                     DVVIWYGLGEGIRDGVLKEVARNIKVFDLDEDRASDLVREVVADFVRDYWKVKLPDGA
                     PARLALYFPSTQAVAELRPQVEAALAAHGLDTSAVLEVNNKSSDKVKSEFYRVAGDPA
                     SPYRVILLVNMGTEGWNCPSLFACALVRKLQSSNNFVLQAATRCLRQVPGNDRPARIY
                     LASANRKPLEAQLSETYGTSIQALNERETERVEKVVELKHPDLPKLLIRRKVLRFRRK
                     QGKTRAKPLKLEKPNVIWTGAGSFQSWQIVDT"
     gene            1943..3265
                     /locus_tag="VFX38_06400"
                     /pseudo
     CDS             1943..3265
                     /locus_tag="VFX38_06400"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(3473..4066)
                     /locus_tag="VFX38_06405"
                     /pseudo
     CDS             complement(3473..4066)
                     /locus_tag="VFX38_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
     gene            3957..4688
                     /locus_tag="VFX38_06410"
     CDS             3957..4688
                     /locus_tag="VFX38_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5314519.1"
                     /translation="MPLKIEPANRIAAPLVAAGVSKEVSSPDGPLAILRDINLAVASG
                     EAVAILGPSGSGKTTLLALLAGLDDASRGEIRLFGETLGALDENRKAALRAGRVGFVF
                     QDFNLLARLTAVENVRIALELAGSADAAARAEEAIAEVGLAARARHYPATLSGGEQQR
                     VAIARAFAPRPQLLFADEPTGNLDSASGARVIELLFDLRAKQGSTLILATHDERLARR
                     CGRRLHLDNGLLHEQEPVRTPGERD"
     gene            complement(4701..6104)
                     /locus_tag="VFX38_06415"
     CDS             complement(4701..6104)
                     /locus_tag="VFX38_06415"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX5314520.1"
                     /translation="MSEVEEQFREGEALLVANAWDSEGFARGLELLRRSAAAGFVEAA
                     VTLGDVHSQTPGLANAAGESAHWYRQAADAGHPVAQDRLADLCMLGRGLPRDDARALA
                     WYARTAAQAYPHALCNLAYMHAEGLGTPPDARAAAELYRRALALADPCGLFNFGLRYA
                     QSDDESLLPAAHACLALAAFANYPLAEGEFARVTARLDSAGAERARGLAADLRARLRA
                     FQERAHSDIALAADPQALLGFAEENLGALDEPALHAAAAATAKGRPHRADPPRDVSAS
                     PRIFTVDSFVSVSECAHFMTLAEDRFTPSRKAMLEHLSGEQSAFSGEAATLLAPDSDA
                     IVRNVERRIAAAFDLDAAHVEPLSVLRYREQDRYATHVDYFDSARLENNRRNGDRGGQ
                     RIASFLVYLRAPETGGETHYLKIGQKIAGRAGMALCHFNLGPDGKPDPLTLHTGEPVR
                     RGEKWLARTTLREHPLF"
BASE COUNT          995 a         2097 c         2070 g         1121 t
ORIGIN      
        1 gtgtccacga tctgccagga ctggaacgag cccgcacccg tccaaatcac attcggcttt
       61 tccagcttca gcggtttcgc ccgggtcttg ccctgcttgc gccggaagcg caggactttg
      121 cgccggatca gaagtttcgg cagatccgga tgcttgagtt cgacgacctt ctccacgcgc
      181 tcggtctcgc gttcgttgag cgcctggata ctcgttccgt aggtttcgga gagctgcgct
      241 tccagcggct ttcggtttgc actcgcaagg tatatccgcg ccgggcgatc gttgccgggt
      301 acctggcgca gacagcgcgt cgccgcctgg aggacaaagt tgttgctgga ctggagcttg
      361 cgcaccagcg cgcaggcgaa tagcgagggg cagttccagc cctcggtgcc catgttcacg
      421 agcaggatca cgcgatacgg cgaggcggga tcgccggcca cgcgatagaa ctcgcttttg
      481 accttgtcgg aggacttgtt gttgacttcg agcacggcgc tcgtgtccag gccgtgcgcg
      541 gcgagtgccg cctccacttg cgggcggagt tccgccaccg cctgcgtact cggaaaataa
      601 agcgcgagcc gcgccggcgc gccgtcgggg agcttgacct tccagtagtc gcggacgaaa
      661 tcggccacca cttcacgcac gaggtcgctc gcgcggtcct cgtccaggtc gaagaccttg
      721 atgttgcgcg cgacttcctt gagcaccccg tcgcggatgc cttcgccgag tccgtaccag
      781 atcaccacgt ccttcagcgg ctggcgttcg aagtaaggcg tgcccgtcgt gttcaccact
      841 atgatgagat tggtgttttt gcccaggtaa tccacggtgc gacgcacctt cttgatctgg
      901 ccgagcagtt tctggccgta ggtgtggtgg gcctcgtcgg aaaacacggc gagatgcggc
      961 agcgaggcga tggcctggag acgcaaattg gcaatctccg cctccggatc gtcgccgggg
     1021 tgcagattga ggcgcagccc accaccgttg cgccgtccgg tcggcttctg gatccggatt
     1081 ttctcggtgt tggtgacgac gacattccac gaactgccgg gcacaatcgg caattccttt
     1141 gcgccgtcgc gggtgaaggt gagcttgagg ctcgccgcga aggtcttgta catccgcggc
     1201 ggcagaatgc gctcgaaggg catgtcggcg atttctcgca gggattcgat gatggtcttg
     1261 cccggcgcga aaatgagcgc gttctggacg aagtcgcctt ccgagtactc ctgcgccatg
     1321 gcgaactcgg tggcgacgat cgtgcccatg aggatggttt tcccggcgcc catcgcgagc
     1381 gcgagaatgt agctcgggta gtcgagcgtc agcgtttcgc gcaaggattc gagtttgtgc
     1441 ttgcgcacaa acacgtcgtc cgttcggatg cgccgaaaca atgcatcttc gttgaaatcg
     1501 gcttgcgcga acagttgcgc gctcaccccc atcgcttcaa gccgagcggt aagcccgtcg
     1561 aagagttgcc gatacaagtc cgggattcgc gccgtgccca tcaccaggcg cagataccag
     1621 taggtttcga gcgcccggaa ctgcgccgcg cgcagatagc gcagctcgcg gcgttcgtcg
     1681 tcggcccagg catagtcgag aatttcggag attgcgggag aatgcgaatg cggataacct
     1741 tccgcgcgcc agactgccac cttgtcggca atcgcttcgt gcaggtgatt catgagccgg
     1801 cagccgcccc ttccgccgag cacagtagcg cgtattggcc ccgcctggca agcggcgcgg
     1861 cggcaacgct cgggcgtgtg aacttgtgcc cgatcggata cctgattatc ctgattgcag
     1921 cccggcgaag gcggaccctc ggatgaaccc cgctggagta cccgaaaccg acggtgcggc
     1981 gatcctcgcg cggctcgatc gcatacccgt ctggccgtgg cgctacaagt tgctgtggat
     2041 tctcggcgcg gcgtatttct tcgccttcta cgacatcgcc aacatcgccg ccgcgctgcc
     2101 ggagatcgtt cgtcagttcg gcgtttccaa cgacgcagcc tcctgggcca tctccgcgag
     2161 tcttctcggc tacatcgtcg gcgcctgggc gatcggcacc atcgccgatc tgtggggccg
     2221 ccatccctgt ctcgcggttt ccgtggccct gttcacgctc ggatcggtcg gcgcggcgct
     2281 ctcgccttcg ctcgcctcgc tgattccggc acgcttcgtc gccgggctcg gcatcggcgc
     2341 cgaaatcgcg acggtcacca cctacctcgg cgagatcgcg cccgccggag tgcgcggaaa
     2401 gtacacgagc tgggcgaacg tctgcgcctt cgccggcttc gcggccgttc ccttcgtcgc
     2461 ccgcgcgctg gtgccgagct tcgcctgggg ctggcgggtg atgtttctga tcggcgcggg
     2521 cggcggcctc acgattttct tcatccgcct gtatctcaag gaaagtccgc gctggctggt
     2581 ggcgcacggc cgcctcgccc ttgccgatgc cctggtccgc gcggcggaac ggcacgcacg
     2641 cgcgcgcatc ggccgagcat tgccggagcc gagcgtggct tgtgccatcg cgaagccgga
     2701 tcgccatcct ctgcgaacgc tccttcgccc gccctaccgc aatcgcctcc tgctcctcac
     2761 ttcactctgg ttctgctact acatcggcaa ctacggctgg ctcggcatgg tgcccgatct
     2821 tctcgcgcat cgcggttatt cgctcaccca aagcctcaac tactggatcg cgagcggagt
     2881 cggttttctc gtcggcgcga tcgcctcgat ctggctgaac gaccgcgtgg aacgcaagca
     2941 cacgcttttc gccgccggga tcgtgtgggt agtggcgctc gccgtcgccg gcttcaatcc
     3001 ttatccgatc cggatcatga cgatcggctt cgtcgcctcc accaccatcg ggctcatggt
     3061 gccgatcctg tacacgctca ccgccgaaca cttcgcgacc ggttcgcgcg cgaccggcat
     3121 ggcaatcgcg aacggcctcg gacatctggg cggtgcgctg gcccccatcc tggtgcttct
     3181 cggggatcag cgctggggat ttctcggtgc cctcgccgtc ttggcggcga gcggaatcat
     3241 cgtgctcctc ttcctgccgt tcaccctgag cattaccgga aaggcgctcg aaagcgccgc
     3301 cgcgcggcga cgctcgcgaa ggctccggcc cgccggtcgc aggcgaagcc gcggcagagt
     3361 gatgcgcggg taccagcaga ctccgcaact gcggccagac gttgtcgagc aacttcggtt
     3421 ccgccacggc ccgcggatgc agcccgtcgg actgcatgag atcgggccgc gtcgcgactc
     3481 cggcgagaag ctcggccacc agcggaacat tgcgggcggc ggagacctcg cgatacgtcg
     3541 cctgaaatcg ctcgcggtag ctcgggccgt aattgggcgg catgcgcacg ccgacaagca
     3601 gcacctcggc accggctcct tgtgcgagcg cgatcatccg ctcgagattg gcgcgcattt
     3661 ccgcgagcgg caggccccga aggccgtcgt tggcgccgag ttcgaggatg aggatcgccg
     3721 ggcgatgccg ggcgagtgcc gccggcagac gcgcgaggcc gcccgcggtg gtgtcgccgg
     3781 gaatgctagc gttgactacg cgataatcgt agccgcgtgc cgccagccgt ttcccgagaa
     3841 gctgcaccca gccgtcttcc gtgggaatgt tgtaggcggc gctcaggctg tcgccgacga
     3901 cgagaatgtt tcgcgctgcg gcggcaaacg cattcgccgc aaaaaggatg aacagaatgc
     3961 cgctgaagat cgaacccgca aaccgcatcg ccgcacctct cgtcgccgcc ggtgtatcga
     4021 aagaggttag cagccccgac ggtccgcttg ccatcctgcg cgacatcaac cttgccgtcg
     4081 cgagcggcga ggcagtcgcg attctcgggc cttcgggctc cggcaagacg acgctgctcg
     4141 ccctgctcgc cgggctcgac gacgccagcc gcggcgagat ccggcttttc ggcgagactc
     4201 tcggcgcgct cgacgagaac cggaaggccg cgctgcgtgc ggggcgcgtc ggcttcgtgt
     4261 ttcaggactt caacctgctt gcgcgtctga cggccgtgga gaacgtccgc atcgcgctcg
     4321 aacttgccgg ctcggccgac gccgcggcgc gcgccgaaga ggcgatcgcc gaggtcgggc
     4381 tcgccgcgcg cgcacgccac tatcccgcga cactttcggg cggcgagcag cagcgcgtcg
     4441 cgatcgcccg tgccttcgcg ccgcgtccgc aactgctctt cgccgacgag ccgaccggca
     4501 atctcgattc cgcgagcggc gcacgcgtga tcgaactgct gttcgatctg cgcgcgaaac
     4561 agggcagcac gctgatactc gcaacccacg acgaacggct cgcacggcgc tgcggccggc
     4621 ggctccatct cgacaacggg cttctgcacg agcaggagcc cgtgcgcacg cccggcgagc
     4681 gggactagcg ggatgcgggg tcagaacagc ggatgttcgc gaagcgtcgt gcgtgcgagc
     4741 catttttctc cgcggcgcac cggctcgccc gtgtgcaggg tcagcggatc cggtttgccg
     4801 tcgggcccga gattgaaatg acagagcgcc atccccgccc gcccggcgat cttttggccg
     4861 attttcaggt aatgcgtttc gccgcccgtc tccggcgcgc gcaggtacac gaggaaggag
     4921 gcgatgcgct gcccgccgcg atcgccgttg cggcgattgt tttcgagccg cgccgaatcg
     4981 aaataatcga catgcgttgc atagcgatcc tgctcgcgat agcgaagcac cgacagcggc
     5041 tcgacatgcg cggcatcgag atcgaaggcg gcggcgatgc gccgctcgac gttgcgcacg
     5101 atggcatcgc tgtcgggcgc gagcagcgtc gcggcctcgc cgctgaacgc gctctgctcg
     5161 ccggaaagat gttcgagcat ggccttgcgc gagggcgtga aacggtcttc ggcgagagtc
     5221 atgaaatgcg cgcattcgct cacgctgaca aaggaatcga cggtgaaaat ccgcggcgag
     5281 gcggagacat cccgcggcgg atcggcgcga tgcggccgac ccttcgccgt cgcggccgcc
     5341 gccgcatgca gtgcgggctc gtcgagcgcg ccgagattct cttcggcaaa gccgagcaat
     5401 gcctgcggat cggccgcgag ggcaatatcg gagtgcgccc gctcctgaaa tgcgcgcagc
     5461 cgcgcacgca gatcggccgc gagtccgcgg gctcgctcgg cgccggcgga atcgagcctc
     5521 gcggttacac gggcgaattc gccctcggcg agcggataat tcgcgaaggc cgcaagcgcg
     5581 aggcaggcat gcgccgccgg caggagcgat tcgtcgtcgc tttgtgcata acgaagaccg
     5641 aagttgaaga ggccgcaggg atcggcgagc gcgagcgcac ggcgatacag ctccgccgcc
     5701 gcgcgcgcgt cgggcggcgt cccgagacct tcggcatgca tgtaggcgag attgcagagg
     5761 gcatgcggat acgcctgcgc cgcggtgcgc gcgtaccacg cgagtgctcg ggcatcgtcg
     5821 cgcggcagcc cgcgaccgag catgcacaga tcggcgagac gatcctgcgc gaccggatgt
     5881 cccgcatccg ccgcctgccg ataccagtgc gcggactcgc ccgcagcatt cgccaggccc
     5941 ggcgtctggg aatgcacgtc gccgagcgtg accgccgcct cgacgaatcc ggcggcggcc
     6001 gatcggcgca aaagttcgag tccgcgggcg aaaccctcgc tgtcccaggc gttcgcgacc
     6061 agcagggcct cgccctcgcg gaactgctcc tcgacttcgc tcatcgccgc agtctagcaa
     6121 agcgcgaacg cccccgagcc gcaatgcaaa gatgcaggcg gtagggatgg atcgcggtcg
     6181 gctttccttt attttgggga cggcgaacgg cgccgcgcac tcgcttctgt ggttccgtcg
     6241 cggtctgcgc ggcgaattgc gtctgccgtt cgcgacactc gta
//
LOCUS       DASUJG010000298         2738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1553784, whole genome shotgun sequence.
ACCESSION   DASUJG010000298 DASUJG010000000
VERSION     DASUJG010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1553784"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..1264)
                     /locus_tag="VFX38_06420"
     CDS             complement(134..1264)
                     /locus_tag="VFX38_06420"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEX5314521.1"
                     /translation="MPLFALGFRPFFLLAGAFAVVAIVLWGFVLAGRGPALGPLWHGH
                     EMLFGYAGAVIAGFLLTAAANWTGRPTLVRLPLAALALLWVAARIVVALGGAPDGLKA
                     ALCAGFFVVLAIGVGRPIVATRKSRNYGVLVVLGVFAALELLYWLAPAMRSRVLDAAL
                     FFLMVLIAIIGGRVIPAFTRNATPGLRVRESGLWRDRLAMASLVALGILALIPQTPRA
                     LFAAVAALGALAHGLRMLGWGGEKVLRRPILWILQVAYFCLPAGLALFAAQALDAPIP
                     PWVPLHVLAVGALGLMTLGMMTRVSLGHTGRLIVAGKTTTLAYLLLIAAVVVRAGGPF
                     LGAASWQTSMLLAVALWSLAFFLFVLAYAPKLLAPRPDGKPG"
     gene            1378..1842
                     /locus_tag="VFX38_06425"
     CDS             1378..1842
                     /locus_tag="VFX38_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314522.1"
                     /translation="MGRQDRSNTGSRSRLSIFVLLALVLAVTTQLVTGFALWSRPGGA
                     LLLAHVIGGAAAIALTLAEWLWLAATRPGRKRLAGFVAAASGPSEWSEAMFLVVTTAT
                     VVLGALLAAALYLGARLPFANLFAAHRALAIAVAALYILHSLIAIATRPRQR"
     gene            2073..>2738
                     /locus_tag="VFX38_06430"
     CDS             2073..>2738
                     /locus_tag="VFX38_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314523.1"
                     /translation="MKNSPLLLASLVLIVPAGIPALAAAPTQPSPASAGKPVDCSAAY
                     GGHTASCRLVPCSKRYLSFVGTWRGKFYAYVRSQSTPEKPVFRPYENSVRYGADDCLE
                     NSAAGESFIVGHETDRYPAFAGLPAKVETSLLIIGRGAGGKAFLRTVDEHGVNSFSLV
                     YRNDAASLAIWKLVLPASTGNPPMTFTTIDGRNFAAANVHTRDVTITMAVGPASAPYW
                     QGVI"
BASE COUNT          509 a          923 c          890 g          416 t
ORIGIN      
        1 gatccggccc tccgcgacat gcaagcgttc ccagcgattc acgcgcgggg cgtgccagcg
       61 gcgaagtcgc tccggaaccg agacggcgga ataagtccgc gaattcccca gcgggcgcag
      121 tcccggcggc ggctcagccg ggtttgccat cggggcgggg cgcgagaagt ttcggtgcgt
      181 aggcaagcac gaagaggaag aatgcgagcg accagagcgc gaccgcgagc agcatcgagg
      241 tctgccaact cgcagcgccg agaaaaggcc cgccggcgcg aaccacgacc gcggcgatga
      301 ggagcaggta ggcaagggtc gtcgtcttgc cggcgacgat gagccggccg gtgtggccga
      361 gcgaaacccg cgtcatcatg cccagggtca tcagtccgag cgcgccgacc gcaagcacgt
      421 gcaatggcac ccagggcggg atcggcgcat caagcgcctg cgcagcaaaa agtgcgaggc
      481 ccgcaggcag gcagaaatag gcgacctgca ggatccagag aatcggtcgg cgcagcactt
      541 tttctccgcc ccagccgagc atccgcaggc cgtgcgcgag cgcgcccagt gccgcgacgg
      601 cggcgaaaag agcgcgcggc gtttgcggga tgagcgcaag gatgccgagg gcgacgaggc
      661 tcgccatcgc gaggcggtcg cgccacaggc cgctctcgcg tacgcgcagg cccggcgtcg
      721 cattgcgggt aaaggcgggg atcacccgcc cgccgatgat cgcgatcagc accatgagga
      781 aaaagagcgc ggcatcgagc acccgcgaac gcattgcggg cgcaagccag tagaggagtt
      841 cgagcgccgc gaatacgccg agcacgacga gcacgccgta attgcggctc ttgcgggtcg
      901 cgacgatcgg ccgcccgacg ccgatcgcga gaacgacgaa aaagcccgcg cagagcgcgg
      961 ccttgagacc gtcgggcgcg cccccgagcg ctacaacgat ccgcgccgcg acccagagga
     1021 gcgcgagcgc cgcgagcggc aggcgcacca gggtcggccg cccggtccag tttgctgcgg
     1081 ccgtgagaag aaatccggca ataaccgcgc ccgcataccc gaagagcatt tcgtggccgt
     1141 gccacaaggg cccgagcgcg gggccgcggc cggcgagcac gaagccccag agcacgatgg
     1201 cgacgacggc gaaggctccg gcaaggagaa agaacggccg gaatccgagc gcgaacaacg
     1261 gcaacgatct cgtttcccgg gaacgatctg gcatcgggat agtgtccaaa atcgctgcat
     1321 cgccggcctt gagccatatc aagatagcag catcgacaca ggcggtacac tttctcgatg
     1381 ggcaggcaag accgcagcaa caccggctcg cgaagccggc tttcgatctt cgtgctgctc
     1441 gcgctggtgc tggcggtgac gacccaactc gtcaccggtt tcgcgctgtg gtcgcgcccg
     1501 ggaggagcgc tccttctcgc gcacgtcatc ggcggcgccg ccgcgatcgc gctcacgctc
     1561 gccgaatggc tgtggctcgc ggcgacccgc cccgggcgca aacgactcgc cggatttgtc
     1621 gccgccgcaa gcgggccctc cgaatggagc gaggcgatgt ttctcgtcgt cacgacggcg
     1681 acggtcgtac tcggtgcgct cctcgctgcc gcgctttatc tcggcgctcg cctgcccttc
     1741 gcaaatctgt ttgcggccca tcgcgcgctc gccatcgccg tggccgccct ctacattctt
     1801 cacagcttga tcgccatcgc gacgcgcccc cggcagcgct gacggccggg ccaggctttc
     1861 cgtgccgcgg agacaaggaa aatgttcctg cggccgcgct cgcaggatga cattcgtcat
     1921 ctgcaagtac tgaccgattg cactggtttg ttccgccgca gtcggcgatc atggaacccg
     1981 gacattcatt tccgctaacg gcagccatct tgcgatcgga agcgatccct tcgttttgca
     2041 tcggtcattt cgccccgctg gagttttccc caatgaaaaa ttctccgttg ctcctcgcct
     2101 cccttgtcct gatcgtgccg gccggcattc ccgcgcttgc ggcggcgccg acgcagcctt
     2161 cgccggcgtc cgcgggaaag ccagtcgact gctcggcggc ctatggcggc cacaccgcct
     2221 cctgcaggct tgttccgtgc agcaagcgat atctctcctt cgtcggaacc tggcggggca
     2281 agttttacgc ctacgtgcgt tcgcaatcca cgccggaaaa accggtattc cgaccctatg
     2341 aaaacagcgt gcgctatggc gccgacgatt gcctcgaaaa ttccgcggcg ggagaaagct
     2401 tcatcgtcgg gcacgagacc gatcgctatc ctgcttttgc cggcctgccg gccaaagtgg
     2461 aaacgagtct cctcatcatc ggccgcggcg ccggcggaaa ggcgtttctg cgcacggtag
     2521 acgaacacgg cgtcaacagc ttctcgctcg tctatcgcaa cgacgccgca agcctcgcga
     2581 tctggaagct cgtgctgccg gcgtcaacgg gcaatccgcc catgacgttc accacgattg
     2641 acggaaggaa tttcgctgca gcgaacgtgc atacgcgcga cgtgacgatc acgatggccg
     2701 tcggacctgc gtccgcaccc tactggcagg gcgtcatc
//
LOCUS       DASUJG010000299         3917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1553839, whole genome shotgun sequence.
ACCESSION   DASUJG010000299 DASUJG010000000
VERSION     DASUJG010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1553839"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..716)
                     /gene="ispD"
                     /locus_tag="VFX38_06435"
     CDS             complement(6..716)
                     /gene="ispD"
                     /locus_tag="VFX38_06435"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX5314524.1"
                     /translation="MPAWALIPAAGTGERFGAGSPKQYLPLAGRPMLAWTLELFLASP
                     RIDGVIVALAAGDRRWSEVAPADPSKPVLTTIGGATRAASVLAALDALAGRLAENDWV
                     LVHDAARACLGPDAIEKLFAELGDDRVGGILATPLADTLKRADGKGRIATTMERAGLW
                     LAQTPQMFRYGLLRSALAAASRAGVAITDEAMAMERAGFAPRLVTGNAGNLKITRADD
                     LALAEALIGVRRMLKSPP"
     gene            complement(741..1082)
                     /locus_tag="VFX38_06440"
     CDS             complement(741..1082)
                     /locus_tag="VFX38_06440"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX5314525.1"
                     /translation="MRWLIILGLAVCLVLLQRALWSTTSSLGHGSELARQVEQLKQEN
                     AKLEQGNSRRWAEVKDLKRGGAAIEEHARMDLGFVKKGETFYQIVKAPAPVSALPNVT
                     APVPASTIHGG"
     gene            complement(1082..1933)
                     /gene="kdsA"
                     /locus_tag="VFX38_06445"
     CDS             complement(1082..1933)
                     /gene="kdsA"
                     /locus_tag="VFX38_06445"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEX5314526.1"
                     /translation="MKLCGFEAGNEQPFFLIAGPCVIESRELVFEVAETLRDIADRLS
                     LRLIFKASFDKANRSSLTSFRGPGLEEGLAILANVRERYRLPVLTDVHEDTPIEEVAA
                     VVDVLQTPAFLCRQTNFIRRVAAAGKPVNIKKGQFLSPWEMGNVVAKAKSTGNEAILV
                     CERGFSFGYGNLVSDMRGIPVMRATGCPVVFDATHSVQQPGALADRSGGQREFVPALA
                     RAAVAAGVAGVFMETHPRPEQALSDGPNAWPLDRMEELLRVLQSVDAAVKAAPYAEAE
                     WFAVSPP"
     gene            complement(1930..3582)
                     /locus_tag="VFX38_06450"
     CDS             complement(1930..3582)
                     /locus_tag="VFX38_06450"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019627041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX5314527.1"
                     /translation="MTRFIFVTGGVVSSLGKGIAAASLGALLEARGLKVTLVKLDPYI
                     NVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFVRTRVSRANNFTAGQVYESVIRKE
                     RRGEYLGGTVQVIPHITDEIKGKILAGADAADICLVEIGGTVGDIESLPFLEAIRQMG
                     VEMGRERAIFLHLTLVPYIAASGEIKTKPTQHSVKELRSIGIQPDVLLCRAAARLPRE
                     ARRKIALFTNVEERAVISGVDAGDIYEIPLLLHEEGLDEIVVRRLRLDVPPADLSDWR
                     RVVEARRHPVHEVDIGMVGKYVDLADSYMSLNQALAHGGLATRTKVNIHYVDSESLQT
                     RGTGELARMDAIVVPGGFGERGIEGKIRAAEFARRHDVPYLGICLGMQTAVVEFSRHV
                     AGLEGAHSTEFAADTPHPVIALISEWQDRGGTTERRDAASELGGTMRLGAQASHLKPG
                     SRVRDIYGRDTLHERHRHRYEFNDNYAARLEAAGLVISGRSDDDLVELIELPAHSWFV
                     GCQFHPEFSSTPRDGHPLFESFVAAALARRERGAAPRRSVQA"
BASE COUNT          661 a         1306 c         1303 g          647 t
ORIGIN      
        1 cgatctcatg gcggtgattt tagcatccgc cgaacgccga tcagcgcttc ggcaagcgcc
       61 aggtcatccg cgcgggtgat tttgagattg cctgcgtttc ccgtcacgag acgcggcgcg
      121 aatcccgccc gctccatcgc catcgcctcg tccgtgattg cgactcccgc tcgcgaggcg
      181 gcggcgagag ccgaccgaag aagaccgtag cgaaacatct gcggcgtctg cgccagccac
      241 agcccggcac gctccatcgt cgtcgcgatc cgtccctttc cgtccgcacg tttcaaggtg
      301 tcggcaaggg gcgtcgcgag aataccgccc acgcgatcgt cgccaagttc ggcgaagagc
      361 ttctcgatgg catcgggacc gaggcaggct cgcgcggcat cgtgcacaag cacccaatcg
      421 ttctccgcga ggcgcccggc gagagcgtcg agtgccgcga gcaccgaggc ggcgcgagtc
      481 gctccgccga tcgtggtaag cacaggcttc gagggatcgg cgggcgcgac ctcggaccag
      541 cgccgatcgc cggcggcgag cgcgacgatc acgccgtcga tgcgcggcga tgcaagaaac
      601 agttcgagcg tccaggcgag catcgggcgg ccggcgagcg gcaggtactg cttcggactt
      661 cccgccccga aacgctcgcc cgttccggcc gcgggaatga gcgcccaggc cggcatgctc
      721 agctccgcgt ccgcgttttc tcaaccgcca tgaatcgtgc tcgcgggcac gggcgcggtc
      781 acgttcggca gggcactcac gggcgccgga gccttgacta tctgatagaa ggtctcgccc
      841 ttcttgacga aaccgagatc catgcgcgcg tgctcctcga ttgcggcacc gccgcgcttc
      901 agatccttga cttcggccca gcgccgcgag ttgccctgct cgagcttcgc gttttcctgc
      961 ttgagctgct cgacctgcct tgccagctcg cttccgtggc cgaggctgct ggtcgtgctc
     1021 catagcgcgc gttgcagcag cacgaggcag acggcgagcc cgagaatgat caaccagcgc
     1081 atcacggcgg cgataccgca aaccactccg cttccgcgta gggcgcggcc ttgaccgcgg
     1141 cgtcaaccga ttgcagcacg cgcaggagtt cctccatgcg atcgagcggc caagcattgg
     1201 ggccgtcgga cagcgcctgc tcgggacgcg gatgcgtttc catgaaaact cccgcgacac
     1261 cggcggcgac cgcggcgcgc gcgagcgccg gaacgaactc gcgctgcccg ccggaacgat
     1321 cggcgagcgc gccgggctgc tgcacgctgt gggtcgcatc gaagacgacc ggacatcccg
     1381 tcgcacgcat caccgggatg ccgcgcatat cggagacgag attgccgtag ccgaagctga
     1441 agccgcgttc gcacaccagg atcgcctcgt tgccggtgct tttcgccttg gcgacgacgt
     1501 tccccatctc ccagggcgag agaaactgcc ccttcttgat gttcacgggt ttgcccgctg
     1561 cggcgacgcg gcggatgaag ttcgtctggc ggcagaggaa ggcgggagtc tggagcacgt
     1621 ccacgacggc cgcgacctct tcgatcggcg tgtcttcgtg cacgtcggtg agaacgggaa
     1681 gccgatagcg ctcgcgaacg ttcgcgagaa tcgcaagccc ttcttccagg cccgggccgc
     1741 ggaagctcgt gagcgaagag cggttcgcct tgtcgaagga tgccttgaat atcagtcgga
     1801 gcgacagtct gtcggcaatg tcgcgcagcg tctcggcgac ctcgaagacg agttccctgc
     1861 tttcgatgac gcagggaccg gcgatgagaa aaaacggctg ttcgtttccg gcctcgaagc
     1921 cgcaaagctt cacgcctgca cgctcctgcg cggtgcggct cctcgttcgc ggcgcgcaag
     1981 ggccgcggcg acgaaggatt cgaacagcgg atggccgtca cgaggcgtcg aagaaaactc
     2041 gggatggaac tggcagccga cgaaccagga atgggcggga agttcgatca actccaccag
     2101 gtcgtcgtcg gaacgccccg aaatcacgag cccggccgct tcgagccgcg ccgcgtagtt
     2161 gtcgttgaac tcgtagcgat gacggtgacg ttcgtgcagg gtgtcgcggc cgtagatgtc
     2221 gcgcacgcgg ctgcccggct tgagatggct cgcctgcgca ccgaggcgca tggtgccgcc
     2281 gagttcgctt gcggcatcgc gccgctcggt ggtgccgccg cgatcctgcc attcggaaat
     2341 cagcgcgatg accggatgcg gagtatcggc ggcaaattcg gtggaatgcg cgccttcgag
     2401 ccccgccacg tggcgagaga attccacgac cgccgtctgc atgccgaggc agatgccgag
     2461 atacggaacg tcgtgccggc gtgcgaattc ggccgcgcgg atcttgcctt cgatgccgcg
     2521 ctcgccgaag cctccgggaa cgacgatcgc gtccatgcgt gcgagttcgc cggttccgcg
     2581 cgtctgcagg ctttcggaat ccacgtaatg aatgttgact ttcgttcgcg tcgcaagccc
     2641 cccgtgcgcg agcgcctgat tgagcgacat gtaggaatcg gcgaggtcca cgtactttcc
     2701 gaccatcccg atgtccactt cgtgcacggg atggcgccgc gcctcgacca cccggcgcca
     2761 gtcggaaaga tcggccggag gcacgtcgag ccggagccgg cgcacgacga tctcgtcgag
     2821 gccctcttcg tgcagcagca gcggaatctc gtagatgtcg cccgcgtcca ctccggagat
     2881 gacggcccgc tcctcgacgt tggtgaagag cgcgatcttg cgccgcgcct cgcgcggaag
     2941 tctcgccgcc gcgcggcaga gcagcacgtc gggctgaatg ccgatcgagc gcagctcctt
     3001 caccgaatgc tgggtgggct tggtcttgat ctcgcccgag gccgcgatgt agggaacgag
     3061 tgtgagatgg agaaaaatcg cgcgttcgcg ccccatctcg acgcccatct ggcgaatggc
     3121 ttcgaggaag ggaagcgatt cgatgtcgcc gacggtgccg ccgatctcga ccagacagat
     3181 gtcggccgca tccgcacccg cgagaatctt ccccttgatc tcgtcggtga tgtggggaat
     3241 cacctgcacc gttccgccga gatactcgcc gcggcgctcc ttgcggatca ccgattcgta
     3301 cacctggccg gcggtgaaat tgttggcgcg gctgacccgc gtgcgcacga agcgctcgta
     3361 atggccgaga tcgagatcgg tctcggctcc gtcctcggtg acgaacacct cgccgtgctg
     3421 gaacggactc atggtgcccg gatccacgtt gatgtaggga tcgagcttga cgagtgtgac
     3481 cttgaggccg cgcgcttcga gcagcgcccc gagcgaggct gcggcaatgc ctttgccgag
     3541 cgaggaaacg accccgccgg tgacgaaaat gaagcgcgtc aaagaacccg gtcccctgtg
     3601 atgcgaccgg gatgacacga tagcaaatcg ccgctccgcg ctcaagcctc gcccgccgca
     3661 ggctttcgcc aaaggacgcg ccgccggggg acgcgtcgcg cccggggcgg ttttgcgcaa
     3721 acccctcgcg ccgcgacgga gcggcggcgc aaccgtcggc gcatcggcct ttcttacaat
     3781 aggcgcgatg gcgacgcccg aaagtcgttt cgcggccggc gaggtcgcgg cgccgaagtc
     3841 acgaacgaac tcccccgagc cgccgcgtgc cggcgcggcg gctgcgctcg cgctcgcacg
     3901 ccgggcgctt gcgctgg
//
LOCUS       DASUJG010000300         3057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1797266, whole genome shotgun sequence.
ACCESSION   DASUJG010000300 DASUJG010000000
VERSION     DASUJG010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1797266"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..1298)
                     /locus_tag="VFX38_06455"
     CDS             complement(132..1298)
                     /locus_tag="VFX38_06455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002708212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX5314528.1"
                     /translation="MIAADLPEPDVDTLAHSARVAEHIRAAIEAAGGAIPFSEYMRLA
                     LYAPGLGYYRAGTEKFGAAGDFVTAPEVSPLFGAVVGRQLAETLAACEGDTVLELGAG
                     SGRLALDVLRTLAAQDRLPARYLILEVSGELAARQRQLLAGEPALAARVSWLDRLPET
                     PVRGVIVANEIADALPVERFRFDDGNVVRLGVKVGKAGFEWAQLPASSELTHRVTKLA
                     ECHDWPEPYESECCPELAPWIAALGDSIECGVLLLFDYGFPEREYYHPQRDTGTLVCH
                     YRHRAHEDPFLWPGLCDITAWVDFSSLAMAGRAAGLDVAGYTTQANFLLGGGIGDAVA
                     AAANDVERYRIAGEIKRLALPSEMGESFKAIALTKDCPAPSAFNFRNLFARLQA"
     gene            1422..2159
                     /locus_tag="VFX38_06460"
     CDS             1422..2159
                     /locus_tag="VFX38_06460"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HEX5314529.1"
                     /translation="MDHLTGRVALVTGGAQRVGAGIVHELHARGMQIMVHYRSSGEAA
                     TRLVDELNARREDSAASVGADLADPQTPQRLVSLLTARFGGLDLLVNSASAFVHAALA
                     ALTVTQWQKLIAANLTAPLFLSRAFAPLLAEHEGTIVNITDAYAARLPAGYAAYAATK
                     AALADLTRSLARELAPKIRVNAVAPGAILWPQPEPEEAAKQKVLASIPLARLGAPDDI
                     ARAVAFLAAEPYLTGVILPVDGGRSLF"
     gene            2263..2493
                     /locus_tag="VFX38_06465"
     CDS             2263..2493
                     /locus_tag="VFX38_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314530.1"
                     /translation="MAHRVNFMLDDAVWQSLQRIGKGERSKVVNKALYEWMKRHRQRG
                     AARRMDELREALPAATASEVAAWIREDRERAR"
     gene            2490..2912
                     /locus_tag="VFX38_06470"
     CDS             2490..2912
                     /locus_tag="VFX38_06470"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX5314531.1"
                     /translation="MRIVVPDASVILKWILADPTGEQDVDKAIALRDAVIGGEIAIRV
                     PSLWIYEVGNILTRKFAKHAGNALAALVAFGMDERRTEGDLLSRTVALTQSCKVTFYD
                     AAYHALALEENGTFVTADARYLRKAAKVGAVTLLSEWK"
BASE COUNT          599 a          954 c          972 g          532 t
ORIGIN      
        1 gctgagcgca gcgaagccca acaatccggc ttgtgatgcc gggaacgtgc gcgaaacgcg
       61 cgtttatccc ggcatgcggg ggcttactgt ccgtgtagac cgacataagc cgcgaagcgg
      121 cgttgccggc gtcaggcctg aagacgggcg aaaaggttgc gaaaattgaa ggccgaaggc
      181 gcggggcagt ctttcgtcag cgcgatggcc ttgaacgatt cgcccatttc cgacggcaac
      241 gcgaggcgct tgatttcgcc cgcgatgcgg taacgttcga cgtcgttcgc cgctgccgca
      301 acggcatcgc cgatgccgcc gccgagcaga aaattcgcct gcgtcgtgta acccgcgaca
      361 tcgagcccgg ccgcgcggcc cgccatcgcc agcgaggaaa aatccaccca cgcggtgata
      421 tcacataacc ccggccacag aaacggatcc tcgtgcgcgc gatggcggta atggcagacg
      481 agcgtccccg tatcgcgctg cggatgatag tactcgcgct ccggaaagcc gtagtcgaac
      541 agcagcagca cgccgcattc gatcgaatcg ccgagcgccg cgatccatgg cgcgagttcg
      601 gggcagcatt ccgattcata aggttcgggc cagtcgtggc attcggcaag tttcgtgacg
      661 cgatgggtga gttcgcttga cgccggcaac tgcgcccatt cgaaacccgc ttttcccacc
      721 ttcacgccga ggcgcaccac gttgccgtca tcgaaacgaa agcgttcgac cggcaaggca
      781 tcggcgattt cgttggcgac gatcacgccg cggaccggcg tctcgggcag acgatcgagc
      841 caggaaacgc gagcggcgag cgcgggttcg cccgcgagga gctggcgttg gcgcgcggcg
      901 agttcgccgc tcacttcgag gatcaggtaa cgcgccggaa gccgatcctg cgccgcgagc
      961 gtgcgcaata catcgagcgc cagccgtccg gagccggcgc ctagttcgag cacggtgtct
     1021 ccttcgcagg ccgcaagagt ttcggcgagc tggcggccga cgacggcgcc gaacaacggc
     1081 gagacttcgg gcgcggttac aaagtcgccg gcggcgccga atttctccgt acccgcgcga
     1141 tagtacccga gtcccggcgc gtacagagca aggcgcatgt actcggaaaa tggaatcgcg
     1201 ccgccagcgg cttcgatcgc cgcccggatg tgttcggcga cgcgcgcgct gtgcgccagc
     1261 gtatcgacgt cgggctccgg cagatcggcc gcaatcatcg ccattcaccc aacttttttg
     1321 cgtccggccg taggttgggc tgagcgcagc gaagcccaac gaccaacgca ggcgagtaaa
     1381 atcaatgagt ggtccacgac atcgttggcg gagggcggaa catggatcat cttaccggac
     1441 gggtggcgct cgtcaccggc ggcgctcagc gggtaggcgc gggcatcgtg cacgaactgc
     1501 atgcgcgcgg tatgcagatc atggtgcatt accgcagttc cggcgaggcg gcgacgcgtc
     1561 tcgtggacga actcaatgcg cgccgcgaag attcggcagc aagcgtcggc gccgaccttg
     1621 ccgatcccca aacgccgcaa cggctcgtga gcctcctcac cgcgcgcttc ggcgggctcg
     1681 atttgctcgt caacagtgcc tcggctttcg tgcacgccgc gcttgccgcg ctgaccgtga
     1741 cgcagtggca gaagttgatt gccgccaatc tcaccgcgcc gctgtttctt tcccgtgcct
     1801 ttgcgccttt gctcgcggaa cacgaaggca cgatcgtcaa tatcaccgac gcctacgccg
     1861 cgagacttcc cgccggctat gccgcctacg cggcaaccaa ggccgcactc gccgacctca
     1921 cccgttcgct cgcgcgcgag ttggccccga agatccgcgt caacgccgtt gcgcccggcg
     1981 cgatcctctg gccgcagccc gaacccgaag aggccgcaaa acaaaaagtg ctcgcgagca
     2041 ttccgctggc acgtctcggc gcgccggacg acatcgctcg agccgtcgcc ttcctcgccg
     2101 ccgagcccta tctcaccggc gtcatccttc ccgtggacgg aggccgttcc ctcttttaag
     2161 gaaccgtggc aaaatctcag cgttcgacaa cgcgtacgac ggaatttgac atcaataata
     2221 cgtaatgata gtgtgtacgc cattacgcat tctgagataa tcatggcgca ccgggtaaat
     2281 ttcatgctgg acgatgcggt atggcaatcc ttgcagcgga tcggcaaagg cgagcgcagc
     2341 aaggtggtaa acaaagcgct ttacgaatgg atgaagcgtc accggcagcg cggggcagcg
     2401 cggcggatgg atgaacttcg tgaggctttg cccgccgcca ctgcttcgga agtcgccgcg
     2461 tggatacgcg aggatcggga gcgcgcgcgg tgagaatcgt ggtaccggat gcctcggtga
     2521 tcctcaaatg gatattggcc gaccccacag gcgaacaaga cgtcgacaag gcgatcgccc
     2581 tgcgcgatgc cgttatcggc ggcgagattg caatcagggt gccgagcctg tggatttatg
     2641 aagttggcaa catactgacg cgaaagttcg caaagcacgc cggaaacgcg cttgcggcgc
     2701 tcgtggcgtt cggcatggac gagcgccgga ccgaaggtga tttgttaagc cgcaccgtcg
     2761 cgttgaccca aagctgcaaa gttacttttt acgatgcggc ttatcacgcg cttgcgctgg
     2821 aagaaaacgg cactttcgtt accgccgacg cgcggtatct tcgcaaagcc gcaaaagtgg
     2881 gcgccgtcac gttgttaagc gagtggaaat aaacctgcct tagcgattgg agagaatggc
     2941 gaagcccttg cccgcgatca agcgtttgtc ggcggtgacg agtttcaggt catagattcg
     3001 ggccgtcgct gcaagaaagc ggtccgcggg atcgttgttc ggtaggcgca cctcgga
//
LOCUS       DASUJG010000301         6124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1431899, whole genome shotgun sequence.
ACCESSION   DASUJG010000301 DASUJG010000000
VERSION     DASUJG010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431899"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3760
                     /locus_tag="VFX38_06475"
     CDS             <1..3760
                     /locus_tag="VFX38_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HEX5314532.1"
                     /translation="TKTMPAAASVAPPDATTPRIVIEGASAHNLKHVDVAIPLGRLVC
                     LTGVSGSGKSTLMEEVLYRAVRRAQGKPTEAPGAHSGLAGAEHFDEVVLVDQSAIGRT
                     TRSNPASYVGAFDAIRKLFAATPLARERDYTLGTFSFNSGAGRCPVCGGNGFEHIEMQ
                     FLSDVYLRCAECDGRRYRDEILEVTIERGGRHVNIAEVLDMTVAGAAAFFADDANVLG
                     GLAPLAAVGLGYLALGQPVPTLSGGEAQRLKLAGRLAAADDGRRVLFLFDEPTTGLHF
                     GDIAVLLAAFRELIARGHSLIVIEHNLEVIRAADWVIDLGPEGGEAGGEVVAEGTPRD
                     VANNRASHTGEALAAYSVVPAQAGTQYRKGSRSETGIQSSLDSGLRRNDEYKGHARGY
                     GGNKNAIEIVNAREHNLKGVNVTIPRDRFTVVTGISGSGKSTLAFDILFAEGQRRYLE
                     SLNAYARSIVAPAARPEVDAITGIPPTVAIEQRTSRGGWKSTVGTLTEIHHFLRLLYV
                     RLSVQHCPDCGTAIEPQSAEAIRARIAKRFRGRRIRIFAPLVVARKGYYTDLAKWAAG
                     KGYTLLRVDGELLPTANWPRLKRFVEHDIELPVAELAIAPANERQLAAALASALEYGK
                     GLARVATGTTEAIYSTRRACLSCGRSFPEPDPRLFSYNSAHGWCTLCTGTGFTGGDED
                     GTPRETCSACHGARLNPVALAASFAGRSIAEVSALAVSDAAHFFQTLKLSARERAIGT
                     DALAEIASRLAFLHEVGLGYLALDRAAPTLAGGEAQRIRLAAQLGSNLSGVCYVLDEP
                     TIGLHPRDHAELLRALKTLRRKGNTVVVVEHDEETIRAAEHLIDLGPSAGIAGGKLVA
                     EGSLAAIQRAPDSITGRALKHPPRHPQFPRRAVSRTDPALTIRGADLHNLRVPQTHIP
                     LGRLVCVTGVSGSGKSSLVRGVLHENLRRVIATKRGRKQPELTGCDAIGGMEAIARVL
                     EVDQTPIGKTPRSCPATYVGIWSDIRNLFSQTPEARARGFDASRFSFNTGAGRCPSCE
                     GQGLKKIEMSFMADVTVTCETCGGARFNRETLEVRWRGKTIADVLTMNVSVATEFFAA
                     QPRIHRALALLDEVGLGYLTLGQQSPTLSGGEAQRLKLVTELAKASSPRGGPTLYLLD
                     EPTVGLHIADVERLTGVLHRLVAAGHSVVVIEHNLDLIAEADWIIDLGPEGGARGGRI
                     VATGAPEQLIKRKTPTARALATFLADPKRRPAGSTASRLIPACNNTSA"
     gene            complement(3852..4256)
                     /locus_tag="VFX38_06480"
     CDS             complement(3852..4256)
                     /locus_tag="VFX38_06480"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX5314533.1"
                     /translation="MDYLLDTNFLIGLWRRPADGPEAGFLEEHADGVLALPWIVKGEF
                     LAGAMVATHKADVVQGFLSRYPVTLPTDTTLTVYARLYAMLHRARKAVSVNDLWIAAS
                     ATEHGLPLLTRNVREFSRIEGLQVVDYAREAN"
     gene            complement(4257..4499)
                     /locus_tag="VFX38_06485"
     CDS             complement(4257..4499)
                     /locus_tag="VFX38_06485"
                     /inference="COORDINATES: protein motif:HMM:NF014723.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VapB family protein"
                     /protein_id="HEX5314534.1"
                     /translation="MKTITLTDEAYERLARLKTSSRDSFSKVVLRAVPKRGTGAQMLR
                     DVRALPPLTREAADAMERAVADQRDPDNWRDPWESP"
     gene            4637..4879
                     /locus_tag="VFX38_06490"
     CDS             4637..4879
                     /locus_tag="VFX38_06490"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX5314535.1"
                     /translation="METIAISKFKSTCLAVLERVRKTGEPVLVTRFGKPVAEIVPPPS
                     PQPQADWLGSLASSGKIVGDIISPATDPEEWEALRD"
     gene            4876..5271
                     /locus_tag="VFX38_06495"
     CDS             4876..5271
                     /locus_tag="VFX38_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012308472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX5314536.1"
                     /translation="MNLLLDTHIWLWSVLDSARLSRKVASALVNPDNQLWLSPVSIWE
                     VLILGEKRRITLNKETPQWIRWALERIPLRQAPLNHEVALAISEVSLPSNDPADRFLA
                     ATARVHDLKLVTTDKRLIAGKGFAVLPNR"
     gene            complement(5364..5741)
                     /locus_tag="VFX38_06500"
     CDS             complement(5364..5741)
                     /locus_tag="VFX38_06500"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEX5314537.1"
                     /translation="MGVFKPQLSIPDTTDDDALQVAALEDLMATKLKVMLQRIESKDY
                     EDIAAMIEAGQSLERGLAIARAMFGNDFQPNESLKALTWLEGGDLSRLGAETKRLLIE
                     TASAVRRLPSVTRTAQTLALPHP"
     gene            5849..>6124
                     /locus_tag="VFX38_06505"
     CDS             5849..>6124
                     /locus_tag="VFX38_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314538.1"
                     /translation="MVEYVAKVRVAEAIGKPLHILVVADIHDLGDDPLRHLERVLGTF
                     PPDVAGGQFLEQVEVLSQLAANIRDAIIVPYIVDLVAKNGAYVGPKVI"
BASE COUNT         1140 a         2002 c         1900 g         1082 t
ORIGIN      
        1 cacaaagaca atgcccgctg cggcaagcgt tgcgccaccc gacgcgacaa caccgcggat
       61 cgtcatcgaa ggcgcgagcg cgcacaacct caaacacgtt gacgttgcga ttccgctggg
      121 tcgcctggta tgcctgaccg gcgtgtcggg cagcggcaag tccaccctga tggaagaagt
      181 tctctaccgc gccgttcggc gcgcgcaggg caagccgacc gaagcgccgg gcgcgcattc
      241 tggacttgcg ggcgcggaac atttcgacga agtcgtgctg gtagatcagt cggcgatcgg
      301 gcgcacgaca cgttcgaatc cggcgagtta cgtcggcgcc ttcgacgcga tccgtaagct
      361 ctttgcggcg acgccgctcg cacgcgaacg cgactacacg ctcggcactt tcagtttcaa
      421 ttcgggcgcc ggccgctgcc cggtctgcgg cggcaacggc ttcgaacaca tcgaaatgca
      481 gtttctctcc gacgtgtatc tgcgttgtgc ggaatgcgac ggccgacgct atcgcgatga
      541 aatactcgaa gtcacgatcg agcgcggcgg acgtcatgtc aacattgccg aagtgctcga
      601 catgacggtg gccggggcgg cggcgttttt tgccgacgac gcgaacgtgc tcggggggct
      661 tgcgccgctc gccgcggtgg gactcggcta tctcgcgctc ggtcagccgg tgccgacgct
      721 ttcgggcggc gaggcgcaac gcctgaaact cgccggccgg ctcgccgccg cggacgatgg
      781 caggcgcgtg ctgtttctat tcgacgaacc gacgacggga ctgcacttcg gtgacatcgc
      841 ggtgttgctc gcggcgtttc gggaactcat cgcgcgcggg cattcgctca tcgtgatcga
      901 acacaatctc gaagtgatcc gcgccgccga ctgggtcatc gatctcggtc ccgaaggcgg
      961 cgaagcgggc ggcgaagttg ttgccgaagg cacgccgcgc gacgttgcga acaaccgcgc
     1021 aagccacacc ggcgaagccc tcgccgccta ctccgtcgtc ccggcgcagg ccgggaccca
     1081 gtatcgaaag ggatcccggt cggagaccgg gattcaatct tcactggatt ccggcctgcg
     1141 ccggaatgac gaatacaaag ggcatgctcg cgggtacggc gggaacaaaa atgcgatcga
     1201 gatcgtcaat gcgcgcgaac acaatctgaa gggcgtgaac gtcacgatcc cgcgcgaccg
     1261 cttcacggtc gtgaccggca tttccggcag cggcaagtcc accctcgcct tcgacatcct
     1321 gttcgccgaa gggcagcgcc gctatctgga atcgctcaac gcatacgcgc ggagcatcgt
     1381 cgcgccggcc gcacgtccgg aggtggatgc gatcaccggc attccgccga cggtcgcgat
     1441 cgaacagcgc acgagccgcg gcggctggaa aagcaccgtc ggcacgctca cggaaattca
     1501 tcatttcctg cgcctgcttt acgtgcgcct gagcgtacag cattgcccgg actgcggcac
     1561 cgcgatcgag ccgcaaagcg cggaggcaat ccgcgcgcgc atcgcgaaac gcttccgcgg
     1621 ccggcgcatc cgtatttttg cgccgcttgt cgtcgcccgc aagggctatt acaccgatct
     1681 tgcgaaatgg gcggcaggaa agggctacac gcttctgcgg gtggacggcg agctgctgcc
     1741 gaccgcgaac tggccgcggc tgaaacgctt cgtcgaacac gacatcgaac ttccggtcgc
     1801 ggaactcgcg atcgctcccg cgaacgaacg ccaactcgcg gcagcgcttg cgagcgcgct
     1861 cgaatacggc aaaggcctgg cgcgggtcgc gaccggaacg accgaagcga tctattccac
     1921 ccgccgggcc tgcttgtcct gcggacgcag ttttcccgaa cccgatccgc ggcttttttc
     1981 ttacaactcg gcgcacggct ggtgcacgct gtgtacgggc acgggattca cgggcggcga
     2041 cgaagacggg acgccacgcg aaacctgttc cgcctgccac ggcgcgcgac tcaatccggt
     2101 cgcgctcgcc gcgagctttg cgggacgttc cattgccgaa gtctcggcgc tcgcggtgag
     2161 cgatgcggcc cacttcttcc aaaccctgaa actttccgcg cgtgaacgcg cgatcggcac
     2221 cgacgcgctc gccgaaatcg cctcgcgcct cgcttttctc cacgaggtcg ggctcggcta
     2281 tctcgcgctc gatcgcgcgg cgccgacact cgcgggcggc gaggcgcagc gcatccgtct
     2341 cgccgcacaa ctcggctcga atctttccgg cgtctgttac gtactcgacg aaccgacgat
     2401 cggcctgcac ccgcgcgatc acgccgagct tctgcgcgcg ttgaagacgc tgcgccgcaa
     2461 aggcaacacc gtggtcgtgg tcgaacacga cgaagagacc atccgcgccg ccgaacacct
     2521 gatcgatctc gggccgagcg cgggcatcgc gggcgggaaa ctcgtcgccg aaggttcgct
     2581 cgccgcgatc cagcgcgcgc cggattcgat caccggccgc gcactgaaac atccgcctcg
     2641 gcatccacag tttccgcgcc gcgcggtctc gcgcaccgat cccgccctca ccattcgcgg
     2701 tgccgatctg cacaatctgc gcgtgccgca aacgcatatc ccgctcggac gcctcgtttg
     2761 cgtgaccggc gtttccggta gcggcaagtc ctccctggtg cgcggcgttc tgcacgaaaa
     2821 cctgcgtcgc gtgatcgcca ccaaacgcgg acggaagcag ccggaactca ccggctgcga
     2881 tgccatcggc ggcatggagg ccatcgcgcg cgtgctcgaa gtggatcaga cgccgatcgg
     2941 caagaccccg cgttcctgcc ccgcgaccta tgtcggcatc tggagcgata tccgcaactt
     3001 gttctcccaa acgcccgaag cgcgggcgcg gggcttcgac gccagccgct tttccttcaa
     3061 caccggcgcc gggcgctgcc cgagttgcga gggccagggg ctgaagaaaa tagaaatgag
     3121 cttcatggcc gacgtcaccg tgacctgcga aacctgcggc ggcgcgcgat tcaatcgcga
     3181 aacgctggag gtgcgctggc gcggcaagac gatcgccgac gtgctcacga tgaacgtgtc
     3241 cgtcgcgacg gaattcttcg ccgcgcaacc acgcatccat cgcgcgctcg ccttgctcga
     3301 cgaagtcgga ctgggttatc tcacgctcgg ccagcagagc ccgacgcttt ccggcggtga
     3361 ggcgcagcgc ctcaaactcg tgaccgaact cgccaaggca agttcgccac gcggggggcc
     3421 cacgctttat ctgttggacg agccgacggt cggtctgcac atcgccgacg tcgaacgcct
     3481 gacgggagtc ctgcatcgcc tggtcgcggc cggccattcc gtcgtcgtga tcgaacacaa
     3541 cctcgacttg atcgccgagg ccgactggat cattgatctc ggccccgaag gcggggcacg
     3601 cggcggtcgc atcgtcgcca cgggcgcccc ggagcaactc atcaagcgca aaaccccgac
     3661 cgcccgcgcc ctcgccactt tcctcgccga cccaaagcgc agacccgccg gcagcaccgc
     3721 ttcgcggctt ataccggcct gcaacaacac ctccgcgtag gcccgcataa gcgaagcgtt
     3781 gcgggcattc ccaacgatac cggcaacgcc tgaccagtgg cgagtggtca atcttagtcc
     3841 acttctgagg gctaatttgc ctcgcgcgcg taatccacga cttgcaaacc ttcgatacgt
     3901 gaaaactcgc gcacattgcg tgttaacagt ggcaagccgt gttctgtcgc gctggcggca
     3961 atccacaaat cgttgacgct gactgccttg cgcgcccgat gcagcattgc gtacaagcgg
     4021 gcatagaccg tcaaggtggt atccgtcggc aacgtgaccg gatacctcga taaaaaccct
     4081 tgaaccacgt cggccttatg ggtcgcgacc atggcgccgg cgagaaattc tcctttgaca
     4141 atccatggca gcgcgagcac gccgtcggca tgttcctcaa gaaaccctgc ttccggtccg
     4201 tccgcaggcc gacgccacag gccgatcaga aaattcgtat ccagcaggta atccattcaa
     4261 ggagattccc agggatcgcg ccagttgtcc gggtcacgct gatccgcgac ggccctttcc
     4321 atggcatcgg cagcttcccg tgtaagcggc ggcaaggcgc gcacgtcacg aagcatctgc
     4381 gcgcccgtcc cgcgcttggg tacggcacgc agaacgacct tggaaaatga gtcgcgcgac
     4441 gaggtcttca gccgcgccag acgctcatag gcttcatcgg tcagcgtaat cgtcttcatg
     4501 cggcaatggt aacacgagtc atgctgtcat gcaattcggc gacccgaccg gcttcgtccc
     4561 agcatagagc ggctaaattg accatagact ttgactatgg tacgatgcgt cagtcagccg
     4621 gaagaatccc gtttttatgg aaacgattgc catttccaaa ttcaaaagca cctgcctcgc
     4681 cgtcctcgag cgggtgcgca agaccggcga gccggtactg gtgacccgct tcggcaaacc
     4741 cgtcgccgaa atcgtgccgc cgccttcgcc gcaaccccag gcggattggt tgggcagtct
     4801 cgcctcgtcg ggaaaaatcg tcggcgacat catctcgccg gctacggatc ccgaagagtg
     4861 ggaggcgctg cgagattgaa tctgctgctg gatacgcaca tctggttgtg gagcgtgctt
     4921 gattccgcgc ggctcagtcg caaggtggcg agcgcgttgg taaatccgga taatcagcta
     4981 tggctgtcgc cggtcagcat ttgggaagtc ttgatactcg gcgaaaaacg ccgcatcacc
     5041 ctgaacaaag aaaccccgca atggatccga tgggccttgg aacgaattcc gctgcggcaa
     5101 gcgccgctca atcacgaagt cgcgctcgcg atttccgagg tgagcctgcc gagcaacgat
     5161 cccgcggatc ggttccttgc ggcgacggcc cgagtccatg acctgaaact ggtcacgacc
     5221 gacaagcgct tgatcgcggg caagggcttc gccgttcttc ccaaccgcta gcgtatttcg
     5281 gaaccggcca atacggaccg acgccttgaa cccggacggc accggaagtc gtcaactttg
     5341 acggcacgcc ggtgcgtcgc ggcttatggg tgcgggagtg cgagggtttg tgcggtccgg
     5401 gtgacggagg gcagccgtcg cacggcactg gcggtttcga tcagcagtcg cttcgtttcg
     5461 gcaccgaggc gactgaggtc gccgccttcc aaccaggtca gggccttgag gctttcattc
     5521 ggctgaaagt cgttgccgaa catggcgcgg gcaatggcga gcccccgctc gagactttga
     5581 ccggcctcga tcatggccgc gatgtcttcg tagtctttgg attcgatgcg ttgcagcatg
     5641 accttgagct tggtggccat gaggtcctca agcgcggcga cctggagcgc gtcgtcgtcg
     5701 gtggtgtcgg ggatggacag ctgcggtttg aaaacgccca ttactcgaat gttcgccgcg
     5761 gcatgggcgg cactgcgccg gcgcgagcga ggcccagccg gaaatgccaa taggcccacg
     5821 agcgcgcatc gaactgaccg gcttcggcat ggtggagtac gtcgcgaagg tacgcgtcgc
     5881 cgaggcgatc ggcaagccgc tgcacatcct cgtagtcgcc gatattcatg acctgggcga
     5941 cgatcctctg cggcatctcg agcgcgtcct cgggactttt ccaccagatg tagcgggcgg
     6001 ccagttcctt gagcaggttg aagtcttgtc gcaacttgcg gcgaatattc gagatgcaat
     6061 tattgtacca tacatcgttg atttggtggc aaaaaacggg gcttatgtcg ggccgaaagt
     6121 catc
//
LOCUS       DASUJG010000302         3309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1309821, whole genome shotgun sequence.
ACCESSION   DASUJG010000302 DASUJG010000000
VERSION     DASUJG010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309821"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            418..1119
                     /locus_tag="VFX38_06510"
     CDS             418..1119
                     /locus_tag="VFX38_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314539.1"
                     /translation="MHKRSIALLLCGLAATLAAGCTVTLVAPYNAQLAAQASAMQADV
                     SSWDFTMRDRAGTVAADPRNSEVMATLNGWRGEADAMLTLAISDDPQTVHCSAIARAT
                     YAAMAGMIPANLRAPESASAAGAASSSGCEAALVADLQSGISDIAAALKWCRVPWVPD
                     AYFAGPADAAPPAAPDADAQTKLTKSCLAEFKAPRTAAGGAPGLGHGRAVSHLLTTLQ
                     AIVYVESRKQAAASQ"
     gene            1157..1504
                     /locus_tag="VFX38_06515"
     CDS             1157..1504
                     /locus_tag="VFX38_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314540.1"
                     /translation="MAIVDDLTRALTDALSAGADAARAQGAALKGDFETLVRPQLEDI
                     AAQAAQIAEDYVHGDIGEEQAEEDLAAQSHRIRPLILAESELVGLAAQTIVNSLLDAL
                     RKTVNTAAGIALL"
     gene            1602..2357
                     /locus_tag="VFX38_06520"
     CDS             1602..2357
                     /locus_tag="VFX38_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018874941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5314541.1"
                     /translation="MDEAASHRQRAGELAALNRSFYDGLWSQARLVAPERFNTWPLVK
                     VLAATAERRLEVAPGLRPRLPLAGTRFLDMSANAVAGLRARGAQATVGLIDAIPFADA
                     SFDLVCACDIVEHVEDDEAALRELARVAAPGATLLLSVPLHEEAWTPFDDLVGHYRRY
                     APGELAGKLDRHGFAVERSAVYGMQPKSSRLLDVGMWYLMHQRERAMWWYNRVIMPLG
                     LRRQKPLRFRTGLIDDDGVDTVLVVCRRLAADS"
     gene            complement(2385..2783)
                     /locus_tag="VFX38_06525"
     CDS             complement(2385..2783)
                     /locus_tag="VFX38_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314542.1"
                     /translation="MDNRQDRRAPREWTAASLSIGVIVIAIGVFFLLYNFGVRVPFMR
                     DHNWWALFILIGAIGPLTMAGKAWRVRGGFDANVLHALTSAAVIVTIALFFFFSVSWN
                     RWWPLFVIYGGLWAISRRSLRHEAPTRPVT"
     gene            2932..>3309
                     /locus_tag="VFX38_06530"
     CDS             2932..>3309
                     /locus_tag="VFX38_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX5314543.1"
                     /translation="MDYELYYWPGIPGRGEFVRLALEEAGAEYLDLARDPRGEKAAIA
                     KMQALMKDPRAIHPSFAPPFLKAGDLLIGQTANILLFLGPRHGLAPPDEAGRLWAHQL
                     QLTLADWIVEIHDSHHPLGASLYY"
BASE COUNT          534 a         1082 c         1143 g          550 t
ORIGIN      
        1 atgtaccgat gaattccgcc atggttcgtt tgccgaggtt catgacattc tccttggata
       61 ggtggaagcc tctgccggcc atccgcgccg gcggcatcag cataagaaaa gatcgcccgt
      121 ttgggaagat gtcggctgcc cgaggcggcg cggatcgaat cgggcatcgt cattccggaa
      181 cggcggagcg gtgtccggat ttccggacga aggaaacgaa tcccgccacg ggcgggatgc
      241 actcttgact gggtcccggc gtccgccggg acgacgacgg agctgctcga tcggcgttgc
      301 acgaaccggt tgctgacttc gccgcagcga cttttccgcc tcgggcttga cggccggatt
      361 gcggacgccc atactcgaag cccttggtca aaccgttccg cggctcgggg agtggcggtg
      421 cacaagcgtt cgatcgctct gcttctttgc ggcttggccg ccacgctcgc ggccggctgc
      481 accgtgacct tggtcgctcc ctacaacgcg caactggccg cgcaggcttc ggccatgcag
      541 gccgacgttt cctcctggga tttcacgatg cgcgatcgcg ccggaacggt cgcggccgat
      601 ccgcgcaact ccgaagtcat ggcgacgctc aacggctggc ggggcgaggc ggacgcgatg
      661 ctcaccctcg cgatcagcga cgatccgcag acggtgcatt gcagcgccat cgcgcgtgcc
      721 acgtatgccg cgatggcggg catgattccg gcgaatctgc gcgcgccgga gagcgcgagt
      781 gccgccggcg ccgcgtcttc ttcgggttgc gaggcggcgc tcgtcgccga tctgcagagc
      841 ggcatctccg acatcgcggc cgcgctcaag tggtgccgcg tcccctgggt gccggatgcg
      901 tattttgcgg gaccggccga tgcagcgccg cccgcggctc cggatgcgga cgcgcagacg
      961 aagctcacga agagttgtct cgccgagttc aaggcgccgc ggaccgcagc gggaggcgcc
     1021 cccggactcg gacacggccg cgcggtgtcc catctgctga cgacgctgca ggccatcgtg
     1081 tatgtcgaga gccgcaagca ggcggcggca tcgcaatgac gattgatgcg cgcgcaaacg
     1141 cacgggagaa atcgtgatgg cgatcgtgga cgatttgacc agggccctga cggacgcact
     1201 ttccgccggg gcggatgcgg cgcgcgcgca gggcgcggcg ctcaagggcg atttcgaaac
     1261 cttggtccgc ccgcagttgg aggacatcgc ggcgcaggcg gcgcagatcg ccgaggacta
     1321 cgttcacggc gacatcggcg aggagcaggc cgaagaggat ctcgcggcgc agagtcatcg
     1381 catccgcccg ctcattcttg ccgaatcgga gcttgtcggg ctcgccgcgc agacgatcgt
     1441 gaattcgctg cttgacgcgc tccggaaaac ggtgaacacc gccgccggca ttgccctgct
     1501 ctgacgcgcg gtttcgcctc gctcccgttt gcggggaatc acccgatcgg cccgtcggac
     1561 gaagcgtcgt acgatattcg acccggcgta cggcggagcg catggacgag gcggcttctc
     1621 atcggcagcg cgcgggcgaa ctcgccgcgc tcaatcgcag tttttacgat ggcctgtggt
     1681 cgcaggcgcg cctcgtcgct cccgaacgtt tcaatacctg gccgctcgtc aaggttctcg
     1741 cggcgacggc ggagcggcgc ctcgaggtcg caccgggact gcgtccgcgc ctgccgctcg
     1801 ccgggacgcg atttctcgac atgagcgcga acgccgtggc gggtctgcgg gcgcggggcg
     1861 cgcaggcgac cgtcgggctc atcgatgcga ttcccttcgc ggatgcgagc ttcgatctgg
     1921 tctgcgcctg cgacatcgtc gagcatgtgg aggatgacga ggcggcgctg cgggaacttg
     1981 cgcgcgtcgc cgcgcccggc gcgaccctgc ttctgtcggt gccgctccac gaggaagcgt
     2041 ggacgccgtt cgacgatctg gtcggacatt atcgccgcta cgcgccgggg gaactcgcag
     2101 gcaaactcga ccgccacggc ttcgcggtcg agcgaagcgc cgtctacggc atgcagccga
     2161 agtcctcgcg gctgctcgat gtcggcatgt ggtacctcat gcaccagcgc gagcgggcga
     2221 tgtggtggta caaccgcgtc atcatgccgc tcggactgcg gcgacagaag cccttgcgct
     2281 ttcgcaccgg tctcatcgac gatgatggcg tggatacggt gctcgtcgtc tgccgccggc
     2341 tcgccgcgga ttcctgagcc gcggtccggc tcagagaagc cgggtcaggt aacgggccgg
     2401 gtcggcgctt cgtgccggag cgagcggcgc gaaatggccc acaggcctcc gtagatcacg
     2461 aagagcggcc accagcggtt ccaggaaacc gagaagaaga aaaacagcgc gatggtcacg
     2521 atcacggccg cggaggtgag cgcgtggagc acgttggcat cgaagccgcc ccgcacgcgc
     2581 caggctttcc cggccatcgt cagcgggccg atcgcgccga tcagaatgaa gagcgcccac
     2641 cagttgtggt cgcgcatgaa aggcacgcgc acgccgaagt tgtagagcag gaagaacacg
     2701 ccgatcgcga tcacgatcac gccgatcgag agactggccg cggtccattc gcgcggcgcc
     2761 cgccgatctt gccgattgtc catctgccgt cccgaagccg gaggtgctgc gtattctgga
     2821 cggaaatcgg cgctgcggca aggcccgcgg tgtccgccgg cgttggcggc ggcgcgacgg
     2881 ccggctgctg cgctatgctc gatcgcgaat gcggaaagaa gcggagcggc gatggactac
     2941 gaactgtatt actggccggg cattcccggg cgcggcgaat tcgtgcggct cgccctcgag
     3001 gaggccggcg cggagtatct cgatctcgcc cgcgatcctc gcggcgagaa ggcggcgatc
     3061 gcgaagatgc aggccctgat gaaagacccg cgggcgattc atccgtcctt cgctccgccg
     3121 tttctcaagg cgggcgatct cctgatcggg cagaccgcga acattcttct ctttctcggg
     3181 ccgcggcacg gtctcgcacc cccggacgaa gccgggcgcc tgtgggcgca tcagttgcag
     3241 cttacgctcg ccgactggat cgtcgagatt cacgacagcc atcatcctct cggtgcgtcg
     3301 ctctactac
//
LOCUS       DASUJG010000303         4544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_819527, whole genome shotgun sequence.
ACCESSION   DASUJG010000303 DASUJG010000000
VERSION     DASUJG010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819527"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1282)
                     /locus_tag="VFX38_06535"
     CDS             complement(<1..1282)
                     /locus_tag="VFX38_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX5314544.1"
                     /translation="MKPVEVFEHPLQGLSLIEASAGTGKTTTLVELYLRALLFAPYTV
                     ESILAVTFTRAATGELSVRIREALAEVRARLDQSGGTNGELQGKLPPRFRTELFARAG
                     NNSEELARRVNAALAAFDDKAVFTIHGFCQRALADNAFEAGAAFATTQTEDEEALLAQ
                     AAADFWRRRSGDEACPAYVHWLLTTVKSPADLLKELRDALTVGAELRVEPTITAAGVQ
                     KAAAAFWQSAEAAKRAWSRGRGVLDPWLRDSDDLNRSKYNKKTTARLLDEWEAWLKES
                     PYPTLPENFDRLTSEKAQSCLKNGAVPNEPFFKQGLGDGLRESAERLCAAWWQETFAS
                     ALQYIREQTRKDKREAREIGFDDMLRNLRDALDAPSGTELAQRLAGRYPLILVDEFQD
                     TDPLQHEIFLRIHESAEKHGLILIGDPKQAIYRFR"
     gene            complement(1279..4515)
                     /gene="recC"
                     /locus_tag="VFX38_06540"
     CDS             complement(1279..4515)
                     /gene="recC"
                     /locus_tag="VFX38_06540"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HEX5314545.1"
                     /translation="MLHIHFGNRIERLADALAAALSVAPPADPLVPETIVVGHAGMDE
                     WLKRAIAGRLGIAANVEFPQPAAFVWRMLRAHDPKLPDRSPLDRGPIVWRLYHALGQA
                     DQAKQVADYLRGKDGLARFELAEALATVFEQYQIYRPEWIHAWEAGRSAVEGDDWQSA
                     LWRRVANGVKQHPARLYQEFITHTDALAPDKPPVRISVFGISALAPEYLKLLVAASGN
                     RDVDLYVPNPSQAYWGDIESEKLLAKWQLTDPKRAEYATSGHPLLAALGSQIRDFIEI
                     LHDIPEEAGNVRSQTWDHFEPAGGESLLARLQNDILELVETPQRLAPDPEDDSILIHV
                     CHSRRREVEVLHDALLAAFEADPELGPEDILVMAPNMDDYAEHIAAVFGAAPPERHIP
                     WSLSERSLRAEHPLAEALLALLALPESRLKASEVLGLLDLPAVARRLELDEQGLESLH
                     RWVGDSGIRWGYDAAHREQLNLPGEAAFTWRFGIDRLLAGYALAPDDDAPWDGIAPWG
                     ALEGQSARALGPLCEFVAQLESWRQSLAADRTFVDWAAAMRELLDLFAPESPDELHVM
                     TMLREAIHELEIQAQLADFGEAVPLPVVRAALASRLGVPHHPRPFLSGEVTFCALAPM
                     RSIPAKLVWLLGMSEDDFPRRGRAPGFDLIAADQRRGDRARRTEDRALFLDALLSARG
                     AFHASYVGRSERDNGELPASVVVNTLREVLGRMGADKGDIVIAHPLQPFSASYQAEPR
                     RGASYARHWLGARDPAAAPVPFAAPLANNAVESLSGSTQIAESIIDIALDDLIAGLCN
                     PARRYLKALGIAHRRPDAAAEDDELFTLNKLDEWRVKDALLARWLETGADFDPDDSFA
                     EFAARGWLPTGTAGQTLYAGCVAETSALVGALFSCVGKQKPAPKDVDRSVGAWHLSGR
                     IADVYPGEGLVCARAGSLRAKDRIGVWIRHLALGTTGEPPAESRFFFVEKGQIKTLRL
                     ASVADPAVHLQQLCELYAASGREPLPLLPDLSFDYIHRLHGRTPDRALPEVRSYWRRW
                     TEGAPNTHVYDGEDFALAFRGVEEPFDEHFARVAQEVFGPIAQAEGTP"
BASE COUNT          779 a         1427 c         1508 g          830 t
ORIGIN      
        1 cgcggaagcg gtagatcgcc tgcttggggt cgccgatcag aatcaggccg tgcttttctg
       61 cgctttcgtg aatgcgcagg aaaatttcgt gctgcagcgg atcggtgtcc tggaattcgt
      121 ccaccagaat caggggatac ctaccggcaa ggcgctgggc gagttcagtg ccgctcggtg
      181 catcgagcgc gtcgcgcaaa ttgcgcaaca tgtcgtcgaa gccgatttcg cgggcttcac
      241 gcttgtcttt ccgcgtttgc tcgcgaatgt attgaagcgc cgaggcgaag gtttcctgcc
      301 accacgccgc gcacagacgc tcggcggatt cccgcaagcc gtcgccgagc ccttgcttga
      361 aaaacggctc gttcggcaca gcaccatttt tcaggcacga ttgcgccttt tccgaggtca
      421 ggcgatcgaa gttttccggc aacgtaggat aaggcgactc cttcagccag gcttcccatt
      481 cgtcgagcag tcgtgccgtt gtttttttgt tgtatttgga acgattcagg tcgtcgctgt
      541 cccgcagcca tggatccagt acgccgcgcc ctcgactcca cgcccgcttc gcggcttctg
      601 ccgattgcca gaacgccgcc gctgctttct ggacgccggc ggcggttatg gtcggttcga
      661 ctcgcagttc ggcgccgacg gtgagcgcgt cgcgcagttc cttgagcaga tccgccggag
      721 atttcacggt cgtcagcaac caatgcacgt aggcgggaca agcttcgtcg ccgctgcgtc
      781 ggcgccagaa atcggccgcg gcctgcgcga ggagcgcctc ttcgtcttcg gtttgcgtcg
      841 tcgcgaaggc tgcgccggcc tcgaaggcat tgtcggcgag cgcgcgctga cagaagccgt
      901 gaatggtgaa gacggccttg tcgtcgaagg cggcgagcgc ggcgttcacg cgcctggcaa
      961 gttcttcgga gttgtttcca gcccttgcaa agagttcggt ccggaacctt ggcggcagct
     1021 tgccctgcaa ctcgccgttc gttccgccgg attgatccag ccgtgcccgc acttcggcga
     1081 gcgcctcgcg gatgcgtacg gacagttcgc cggtagccgc gcgggtaaag gtcaccgcga
     1141 gaatgctttc aacggtgtac ggtgcgaaca gcagtgcacg cagatacaac tcgacgagcg
     1201 tagtcgtctt gccggtaccc gcgctcgcct cgatgagcga cagaccctgc agcgggtgtt
     1261 cgaatacttc gaccggtttc acggtgtgcc ctcggcctgc gcgatcgggc cgaacacttc
     1321 ctgcgccacg cgggcgaagt gttcgtcgaa cggctcctcg acaccccgga acgcgagcgc
     1381 gaagtcttcg ccatcataga catgagtatt cggcgcgccc tcggtccatc gtcgccagta
     1441 agaacgaact tccggaaggg cgcgatccgg tgttctaccg tgcagtcgat gaatgtaatc
     1501 gaaggacaga tcgggcagca gcggcaacgg ttcgcgacca ctggccgcgt acagttcgca
     1561 cagttgctgc aaatgaacgg cgggatctgc cacggacgcc agccgcaaag tcttgatttg
     1621 tcctttctcg acgaagaaaa agcggctttc ggccggcggc tcgccggtgg ttcccagtgc
     1681 gagatggcgg atccaaactc cgatgcggtc tttggcacgc agacttccgg cgcgtgcgca
     1741 aacgagtcct tcgccgggat agacgtcggc gatgcgcccg ctaaggtgcc acgcgcccac
     1801 cgatcgatcg acgtctttcg gcgcgggctt ttgcttgccg acgcaggaaa acagggctcc
     1861 gaccagcgcg ctcgtttcgg ccacacaccc ggcatacagg gtttgccccg ccgtgccggt
     1921 aggcagccat ccgcgggcgg cgaactcggc gaaggaatcg tcgggatcaa agtccgcgcc
     1981 ggtttcgagc cagcgggcga gaagagcgtc cttcacccgc cattcgtcga gcttgttgag
     2041 ggtgaacagc tcatcgtctt cggctgcggc gtccggccga cggtgcgcga ttccgagcgc
     2101 cttgagataa cgccgcgcgg gattgcacaa gcctgcgatc aggtcatcca gcgctatgtc
     2161 aatgattgat tccgcgatct gtgtgctgcc cgaaagcgat tcgacggcat tgttcgccag
     2221 gggcgcggcg aaggggaccg gcgcggcggc gggatcgcgc gcgcccagcc aatggcgcgc
     2281 atagctcgcc ccgcgtcgcg gctcggcttg atagctcgcg gaaaacggtt gcagcggatg
     2341 cgcgatgacg atgtcgccct tgtcggcgcc catgcgcccg agcacttcgc gcagcgtgtt
     2401 gacgacgacg gaagccggca gctcgccgtt gtcgcgctcg ctgcgcccga cgtagctcgc
     2461 atggaaggcg ccgcgagcgc tgaggagtgc atcgaggaac agtgcgcggt cttccgtgcg
     2521 ccgggcgcga tcgccgcgtc gctggtctgc ggcgatgagg tcgaatcctg gggcacgccc
     2581 gcggcgcggg aaatcgtcct cgctcatgcc gagcagccac acgagtttgg ccggaatgct
     2641 gcgcatcggg gcgagggcgc agaaggtgac ttcgcccgag agaaacggcc gcgggtgatg
     2701 cggcacgccg agccgtgacg cgagcgcagc gcgcacgaca gggagcggca cagcttcgcc
     2761 gaagtcggca agttgcgcct gaatttcgag ttcgtggatc gcctcgcgca gcatcgtcat
     2821 gacatgcagt tcgtcaggcg attccggcgc aaacagatcc agcagttcac gcatcgccgc
     2881 ggcccagtcg acgaacgttc gatccgcggc gagagattgg cgccacgatt ccagttgcgc
     2941 gacgaattca cacaagggcc ccagtgcgcg cgccgactga ccttcgagcg caccccaggg
     3001 cgcgatccca tcccacggcg catcgtcgtc cggcgcaagc gcatagcccg caagcaggcg
     3061 atcgatgccg aaacgccagg taaaagccgc ctcgccgggc agattcaact gctcgcggtg
     3121 cgcggcatcg tagccccagc ggatgccgct gtcgccgacc cagcggtgga ggctttcgag
     3181 tccctgctcg tcgagttcga ggcggcgcgc gacggcgggc agatcgagca ggcccaacac
     3241 ttcgctggct ttcaaccgcg attcggggag cgcgagcagc gcgagcagcg cttcggcgag
     3301 cggatgttcg gcgcgcaggc tgcgttcgct gaggctccag ggaatgtggc gctccggcgg
     3361 cgccgcgccg aacacggcgg cgatgtgttc cgcgtagtcg tccatgttcg gcgccatcac
     3421 gaggatgtct tccggtccga gttcgggatc ggcctcgaag gcggcgagca gcgcgtcgtg
     3481 cagcacctcg acctcgcgcc ggcggctgtg gcagacgtga atcaggatcg aatcgtcttc
     3541 ggggtccggc gccagacgtt gcggagtttc gaccagttcc aggatgtcgt tctgcaatcg
     3601 tgcaagcagg ctttcgccgc cggcaggttc aaagtgatcc caggtctgcg atcggacgtt
     3661 tccggcttct tccgggatgt cgtgcagaat ttcaatgaag tcgcgaatct ggcttccaag
     3721 cgcagcgagc aacgggtggc cgctggttgc gtattccgcc cgtttcggat cggtgagctg
     3781 ccatttggcc agaagctttt cgctttcgat gtcgccccag taggcctgcg acggatttgg
     3841 cacgtagaga tccacgtcgc ggttgcctga agcggcgacg agcagtttca gatattccgg
     3901 cgcgagcgcc gaaataccaa acaccgaaat tcgcacggga ggcttgtccg gcgcgagcgc
     3961 atcggtatga gtaatgaact cctggtacag acgcgcggga tgctgcttca ctccattggc
     4021 cacgcgccgc cacagcgccg actgccagtc gtcaccctcg acggcgctgc gcccggcctc
     4081 ccaggcgtga atccattcgg ggcggtaaat ctggtattgc tcgaacaccg tcgccagcgc
     4141 ctcggcgagt tcaaagcggg cgagaccatc cttgccgcgg aggtaatctg ctacctgctt
     4201 cgcctgatcg gcttggccaa gcgcgtggta gagccgccag acgatcggtc cgcgatcgag
     4261 cggggaacgg tcgggcagtt tggggtcgtg tgcacgcaac atgcgccaga cgaacgccgc
     4321 cggctgcggg aattcgacat tggcggcgat gccgagccgc ccggcgatcg cgcgcttcag
     4381 ccactcgtcc atgccggcat ggccgacgac gatggtttcg ggcacaagcg gatcggcggg
     4441 cggcgcgacc gagagcgccg cggccagcgc gtccgcgagg cgttcgatgc ggttgccgaa
     4501 atgaatgtgc agcaaggttt tgcgctaccg atgagaatcc gcgg
//
LOCUS       DASUJG010000304         6095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_698094, whole genome shotgun sequence.
ACCESSION   DASUJG010000304 DASUJG010000000
VERSION     DASUJG010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6095
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698094"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1328)
                     /locus_tag="VFX38_06545"
     CDS             complement(<1..1328)
                     /locus_tag="VFX38_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017356415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5314546.1"
                     /translation="MNSTLEKLEQIRSRTGRANAQGLSDATIVRFAASDPKLLAAVGE
                     AERELDRLEREFPQLLRQDEATQAHAIQADFVNFYPDDAVNPYVPLAARGPWIVTVGG
                     AVIHDNGGYGMLGLGHAPEGVLAAMNGTQVMANVMTPNYSQLRLARALRREIGQTRGE
                     CPFARFLAMNSGSEAVAVGGRIADVNAKLMTDPGARHAGREIRQLSLGGGFHGRTERA
                     ARYSDSSRAIYRKHLASFRDRDTLWTVPPNDLAALRAAFGRAEREKVFLEAFFIEPVM
                     GEGDPGLAITPEFYRLARELTEAHGSLLLVDSIQSGLRACGLLSIVDYPGFERERAPD
                     METYSKALNAGQFPLSILAMTERAAGLYRKGVYGNTMTANPRAMDVAVAVLEAVTPEL
                     RANIRRRGEEFLQRFKELQRELDGMITKVQGTGLLFSCELAPQYKIFGAGS"
     gene            complement(1680..1756)
                     /locus_tag="VFX38_06550"
     tRNA            complement(1680..1756)
                     /locus_tag="VFX38_06550"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1720..1722),aa:Lys,seq:ctt)
     gene            complement(1937..2012)
                     /locus_tag="VFX38_06555"
     tRNA            complement(1937..2012)
                     /locus_tag="VFX38_06555"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1977..1979),aa:His,seq:gtg)
     gene            complement(2089..2164)
                     /locus_tag="VFX38_06560"
     tRNA            complement(2089..2164)
                     /locus_tag="VFX38_06560"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2129..2131),aa:Arg,seq:tct)
     gene            complement(2279..4324)
                     /gene="uvrB"
                     /locus_tag="VFX38_06565"
     CDS             complement(2279..4324)
                     /gene="uvrB"
                     /locus_tag="VFX38_06565"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018946005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX5314547.1"
                     /translation="MADQFRLRADYRPAGDQPEAIAALLRGLENGLARQVLLGVTGSG
                     KTFTVANVIATLQRPALVLAPNKTLAAQLYGELREFFPHNAVEYFVSYYDYYQPEAYV
                     PASDTYIEKDASINEHIEQMRLSATKAMMERRDAVIVATVSAIYGLGDPELYLSMVLH
                     LDRGERVDQREILRRLAELQYQRNETDFRAGTYRVRGEVIDVFPAESEREAVRIELFD
                     DEVESLSYFDPLTGEVERSVPRLTVYPRTHYVTPRETLLDAVDKIRVELAQRLQDLRR
                     DNKLVEAQRLEQRTRFDMEMILEIGYCTGIENYSRHLSGRAAGEPPPCLFDYLPDDAL
                     LFIDESHVTVPQLNGMYRGDRSRKETLVEYGFRLPSALDNRPLSFEEFDRISPQTVYV
                     SATPRPYEMGRADQIVEQVVRPTGLVDPQVELRPVVHQVEDLLSEVRERADRRERVLV
                     TTLTKRMAEDLTEYLNEHGARARYLHSDIDTVERSELVRGLRLGDFDCLVGINLLREG
                     LDLPEVSLVAILDADKEGFLRSEGSLIQTIGRAARNLNGRAILYADAMTDSMRRALEE
                     TERRRTKQRAYNREHGITPQGIRKAVLDVMEAAYGTRGTKLNSFSRAAEERAEYLRLP
                     EAERERRLAAMEKEMYEHARNLEFEQAAKLRDEINRARELAWGPGRKAAAGTPETAS"
     gene            4446..5633
                     /locus_tag="VFX38_06570"
     CDS             4446..5633
                     /locus_tag="VFX38_06570"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019593142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX5314548.1"
                     /translation="MEPDIAQRLAAVSASPTLAISALAKDLKAQGRDVLDLSAGEPDF
                     ITPAHIRAAGIAALERGDTHYTATDGTPALKSAIAAKFSRENRLEFTAEEIIATAGVK
                     QALFNLCLALLDPGDEVLIPAPYWVSYPTMAQLAGAEPRILAGDPASGFKLAPATLAA
                     ALTPRTRLLFLNSPCNPSGASYSRRELDGLAEVLRTRPDVLVATDDIYEHLIYGDEEF
                     ANILMAAPDLRDRCVVVNGVSKSYAMTGWRIGYAAGPVWLIKAMKKLQSHSTSNPAAV
                     SQAAAAAALAGPQDCVREMKEAFRARRDRFVAGLNRIRGLRCALPDGAFYAFADCREA
                     MAAREIGSDGDFAAFLLREAGIAAVPGGAFGGPGHLRFSYACDQSTLDAALERLDSTL
                     GES"
     gene            complement(5676..>6095)
                     /locus_tag="VFX38_06575"
     CDS             complement(5676..>6095)
                     /locus_tag="VFX38_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5314549.1"
                     /translation="SRPMVGALSTGLAQRALDESVAYAKGRQSFGRPIHEHQGVGFKI
                     SEMGMRTEAARLLTRQAAWRKDRGEDNTLYSSYAKAFGSDTAMWAATEAVQILGGYGY
                     SKEYPAEKLMRDAKVMQIYEGTNEIQRLVMLRQLTRA"
BASE COUNT         1047 a         2022 c         1923 g         1103 t
ORIGIN      
        1 gaaccggcac cgaagatctt gtactggggt gcgagctcac agctgaacag cagacccgtc
       61 ccctgcacct tcgtgatcat gccgtcaagt tcccgttgca gctccttgaa tctctgaaga
      121 aattcctcgc cgcggcggcg aatgttcgcg cgcagttccg gtgttaccgc ctccagcacc
      181 gcaaccgcca catccatggc ccgcgggttg gccgtcatgg tgttgccgta gacgcccttg
      241 cgatagagtc ccgccgcgcg ctccgtcatg gcaagaatgg agagcgggaa ctggccggcg
      301 ttgagcgcct tggaataggt ttccatgtcc ggtgcccgtt cgcgttcgaa accgggataa
      361 tcgacgatcg aaagcagacc gcaagcgcgc agtccggatt gaatcgaatc gaccagcagc
      421 agggagccgt gggcttcggt cagttcgcgg gcgaggcgat agaactccgg cgtgatcgcc
      481 agtcccggat cgccctcccc catcacgggc tcgatgaaaa acgcctcgag aaaaaccttc
      541 tcgcgttcgg cgcgtccgaa ggccgcacgc agagcggcga gatcgttcgg cgggacggtc
      601 cacaaggtat cgcgatcgcg aaaactcgcg agatgtttgc gatagatggc gcgcgacgaa
      661 tccgaatagc gggcggcgcg ctcggtccgt ccgtgaaatc caccgccgag cgacagttgg
      721 cgaatctcgc gccctgcgtg gcgcgccccc ggatccgtca tgagcttggc gttgacgtcg
      781 gcgatgcgcc cgcccacggc gaccgcctcc gaaccgctgt tcatcgcgag gaatctcgcg
      841 aacgggcatt ccccgcgcgt ctgtccgatt tcgcggcgca gcgcacgcgc cagacgcaat
      901 tgcgaataat tcggcgtcat gacattcgcc atgacctgcg ttccgttcat tgccgcgagg
      961 acgccttccg gcgcgtggcc gaggccgagc atgccgtagc cgccgttgtc gtgaatgacc
     1021 gcaccgccga ccgtcacgat ccacggcccg cgggccgcga gcgggacata aggattcacc
     1081 gcatcgtcgg gatagaagtt gacgaaatcg gcttgaatgg catgagcctg cgtcgcctcg
     1141 tcctgtcgca gaagttgcgg aaactcccgc tccaggcgat cgagttcgcg ttccgcctcg
     1201 ccgaccgccg caagcaactt cggatcgctt gcggcaaagc gcacgatggt tgcgtcggaa
     1261 agtccctgcg cgttggcgcg cccggtgcgc gaacgaatct gttcaagttt ctcgagtgtg
     1321 gaattcatgg ccatctccaa tcgtcaatgc cggcaccgtt tgccatctct cgtgaacgcc
     1381 cgccggcagg ccggaacgga tacgcgtcgc acagccgacg gggtttgaaa attcaaggca
     1441 accaggtcac agctctgccc gccgagcgga aagccgcttt tcacgctttc cggaagccct
     1501 cgaaagccgc gcttttccgc atcctttcaa tggccaatta tagcattgca ggggcctttc
     1561 ccgtcttcgc cacggtgttc tgacgcccat gcaccacgca gaaatcctgc gtccaaatcg
     1621 gcgcggccac ctgtggagcg tgacgtcgtc gctctcgaag gaaaacgtgt tgcttttgtt
     1681 ggtgggccgt gaaggaatcg aaccttcaac cttgggatta agagtcccct gctctgcctg
     1741 attgagctaa cggcccatgt aatcaagtcg gcgacggggc atcctgccgc cgcgcctcca
     1801 cgccgtccga aaacaaagtt tccgtacggc ttcgcagcac tgcgcactgc gcgggtgcgt
     1861 cctgcacccg atcgttactt ccgcgacggg gatcctgccg ccgcgcgagc gatccttgtg
     1921 gcaccgtcct tgtcaatggg gtggacgatg ggatttgaac ccacgaccac cgaggccaca
     1981 acccggtgct ctaccagctg agctacgtcc accatcacgc catcgtgccg cggctggccg
     2041 cgatgctgcg cgtagtatag cgaaggcttt gccggcagcg cccgcatctg gcgcgcccgg
     2101 caggaatcga acctgcaacc tgcggcttag aaggccgctg ctctatccat tgagctacgg
     2161 gcgcgcggag ccgttccgcg gccattgtag cggtcgcgcg gagccggccg aagcccgcct
     2221 cggccgttct ttcgcacgag aagtcggaaa ccgctcgcgc ccgaaacgca gaagaaggct
     2281 acgaggccgt ttccggcgtt ccggcggcgg ccttgcggcc cgggccccag gcgagttcgc
     2341 gggcgcggtt gatttcgtcg cgcagcttcg ccgcctgctc gaattcgaga ttgcgggcgt
     2401 gctcgtacat ttccttttcc atcgccgcga ggcgccgctc ccgttcggct tcgggcaagc
     2461 ggaggtactc cgcacgctct tccgccgcac gcgagaagga attcagcttc gtgccgcgcg
     2521 tgccgtaggc cgcctccatc acgtcgagca cggctttgcg gattccctgc ggggtgatgc
     2581 catgttcgcg gttgtaggcc cgctgtttcg ttcgccgcct ctcggtttcc tccagcgcgc
     2641 ggcgcatgga gtcggtcatc gcgtccgcgt agagaatggc ccgcccgttc aaattgcgcg
     2701 cggcgcgtcc gatcgtctgg atgagggaac cctcggagcg caggaatcct tccttgtcgg
     2761 cgtcgagaat cgccaccagc gacacttccg gcagatcgag accttcgcgc agcagattga
     2821 tgccgacgag acaatcgaaa tcgccgagac gcaacccgcg cacgagttcg gagcgctcga
     2881 ccgtgtcgat gtcggagtgc agatagcgcg cgcgcgcgcc gtgttcgttg agatattcgg
     2941 tgaggtcctc cgccatgcgt ttggtgagcg tggtgacgag cacgcgctcg cgccgatcgg
     3001 cccgttcgcg cacctcggag agaagatcct cgacctgatg cacgaccggg cgcagctcga
     3061 cttgcgggtc cacgagtccc gtcgggcgga ccacctgctc cacgatctga tccgcgcggc
     3121 ccatctcgta aggccgcggc gtcgccgaga cgtacaccgt ctgcggcgaa atccggtcga
     3181 attcctcgaa gctcaagggg cggttgtcga gagcggaagg caggcggaat ccgtattcaa
     3241 cgagggtttc cttgcgcgag cggtcgccgc gatacatccc gttcagttgc ggcacggtca
     3301 cgtggctctc gtcgatgaaa aggagcgcat catccggcag ataatcgaaa agacagggcg
     3361 gcggctcgcc cgcggcgcgc ccggacagat ggcgcgaata attctcgatc ccggtgcaat
     3421 agccgatctc gaggatcatt tccatgtcga atcgggtgcg ctgctcgagc cgctgggcct
     3481 cgacgagctt gttgtcgcgg cgaagatcct gcagccgttg tgcaagttcg acgcgaatct
     3541 tgtccacggc gtcgaggagc gtctcgcggg gcgtgacgta gtgcgtgcgc ggatatacgg
     3601 tgagacgcgg cacgctgcgc tcgacctcgc ccgtaagcgg atcgaaatac gacagcgact
     3661 cgacctcgtc gtcgaagagt tcgatccgca ccgcctcgcg ttcgctctcc gccgggaaga
     3721 cgtcgatcac ctcgccacgc acgcggtagg ttccagcgcg aaaatcggtt tcgtttcgct
     3781 ggtactggag ttccgcgagt cgccgcagaa tctcgcgctg atcgacccgt tcgccgcgat
     3841 cgagatggag caccatgctc agatagagtt ccggatcgcc caggccgtaa atcgcggaga
     3901 cggtcgcgac gatcaccgcg tcgcgccgtt ccatcatcgc cttggtcgcc gaaagacgca
     3961 tctgctcgat atgctcgttg atcgaagcgt ccttctcgat gtaggtatcg gacgcaggca
     4021 cgtaggcttc cggctgatag tagtcgtaat acgagacgaa atattcgacg gcgttgtgcg
     4081 gaaagaactc gcgcagttcg ccgtacagtt gcgccgcgag cgtcttgttg ggcgcaagca
     4141 ccagcgcggg gcgctgcagc gtcgcgatca cattggcgac ggtgaacgtt ttgcccgaac
     4201 cggtgacgcc gaggagcacc tggcgcgcaa gcccgttctc gaggccgcgc aggagcgcgg
     4261 cgatcgcctc gggctgatcg ccggcgggcc ggtaatcggc ccgcagtcgg aattgatcgg
     4321 ccatggcggg aagaccggcg ggcaactcgt tattataaaa gccgccgcgc gcccggttcc
     4381 gcactccggc gcgcgcaagc agcctcgccg cagcccgtat ttcgtccaat cctgcaggag
     4441 actccatgga gcccgatatc gcgcaacgcc tcgcggccgt gagcgcgtcg cccaccctcg
     4501 ccatcagcgc cctcgccaag gacctcaagg cgcaggggcg cgacgtgctg gatctctcgg
     4561 ccggcgagcc cgatttcatc acgcccgccc atatccgcgc cgccgggatc gcggcgctgg
     4621 agcgcggcga cacgcactac accgcgaccg acggcacacc cgcgctgaag agcgcgatcg
     4681 ccgcgaagtt ctcccgcgag aaccggctcg aattcacggc cgaggaaatc atcgcgaccg
     4741 cgggggtcaa gcaggcgctg ttcaatctct gcctcgcgct cctggatccc ggcgacgagg
     4801 ttctgatccc ggcaccctat tgggtttcgt atccgacgat ggcgcagctc gccggagccg
     4861 aaccgcgcat ccttgccggc gatcccgcga gcggtttcaa actcgctccc gcgacgctcg
     4921 cggcggcgct caccccgcgg acgcggctgc ttttcctcaa cagcccctgc aatccttccg
     4981 gcgccagtta cagccgcagg gaactcgatg gcctggccga agttctgcgt acgcggcccg
     5041 atgttctggt tgcgaccgat gacatctacg agcacctgat ttatggcgac gaagagttcg
     5101 ccaacattct catggcggct cccgatcttc gcgatcgctg cgtcgtcgtc aacggcgtgt
     5161 cgaagtccta tgcgatgacg ggctggcgca tcggctacgc cgcggggccc gtctggctga
     5221 tcaaggccat gaaaaagctc cagtcgcaca gcacctcgaa tccggcggcg gtgtcccagg
     5281 ccgccgccgc ggcggccctc gccggtccgc aggattgcgt gcgcgaaatg aaggaggcct
     5341 tccgcgcccg gcgcgatcgc tttgtcgccg ggctcaaccg catccgcggc ctgcgatgcg
     5401 cgctacccga cggcgcgttc tacgcctttg ccgattgccg cgaagccatg gctgcacggg
     5461 aaatcggaag cgacggcgat ttcgctgcct ttctgctccg ggaggcgggg attgccgcgg
     5521 taccgggcgg cgctttcggc ggtcccgggc atctgcgctt ctcctacgcc tgcgatcaga
     5581 gcacgctgga tgcggcgctg gagcgtctcg actccacgct cggagaaagc tgaaccgacc
     5641 gggcgagccg gccgtcacca gcaggcgcgg ctttctcagg cgcgggtgag ctgccgcagc
     5701 atgacgaggc gctgaatctc attggtgccc tcgtagatct gcatgacctt ggcgtcgcgc
     5761 atgagctttt cggccggata ctccttgctg tagccatagc cgccgagaat ctgcaccgct
     5821 tccgtcgccg cccacatggc ggtgtcggag ccgaaggcct tggcatagga ggaatacagc
     5881 gtgttgtcct cgccgcgatc cttgcgccac gccgcctgac gtgtaagaag gcgcgcggcc
     5941 tcggttcgca tccccatctc ggagatcttg aatccgactc cctgatgctc gtgaatgggc
     6001 cggccgaacg actggcgccc cttcgcgtag gcaaccgact catcgagcgc gcgctgcgcc
     6061 agtcccgtgg acagcgcccc caccatcgga cgcga
//
LOCUS       DASUJG010000305         3243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_820328, whole genome shotgun sequence.
ACCESSION   DASUJG010000305 DASUJG010000000
VERSION     DASUJG010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820328"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VFX38_06580"
     CDS             <1..563
                     /locus_tag="VFX38_06580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5314550.1"
                     /translation="SPPLKVLYAGRGGRQKRVWLLNRIVEYFVPTGLPIEFHFAGTMA
                     DELSPTARDAAIVHGEIGNRTEMQALYAASHVVILVSGFEGFPMVVKEGMASSCVPVV
                     TALPGYQMHLTDHENALLIEAVDDEVEVVRLAIARLEELMADRALVAKLSTNAKQYAR
                     RNFGMEAFRAKYRELLLAPLARSDRM"
     gene            607..1512
                     /locus_tag="VFX38_06585"
     CDS             607..1512
                     /locus_tag="VFX38_06585"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX5314551.1"
                     /translation="MPSDKTFVFVAGLHHSGTSLLHRLLGAHPEISAFSGTGVPEDEG
                     QHLQSLIPPALAYGGPARFGFDPRARLDETSPLATPLGAEKLYGEWAPHWDLEKRLLI
                     EKSPPTLVRLRFFQALFPHSVFVVILRHPIALAVATRRGFVRYTLRNSFSVGRRGGKS
                     GPAASAPLHLRDLAKLRLRRDVEHTLICYEQAFRDLGHIDRAFVLRYEDFVADPNAML
                     RRVFRFLGVAPVEAAEAVRRDVNARYVHAWRRGLLAPLRARIAARFEERARAVGYSLV
                     EPARQVPLSVSGSDVTVAEGPAAGS"
     gene            1593..2264
                     /gene="rpe"
                     /locus_tag="VFX38_06590"
     CDS             1593..2264
                     /gene="rpe"
                     /locus_tag="VFX38_06590"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020681687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX5314552.1"
                     /translation="MPEFWISPSVLSADLARLGEESAAVLRAGGERIHFDVMDNHYVP
                     NLTFGPSVLKALRAYGIEAPIDAHLMVTPTDRLIRDFLDAGAATILIHPEATEHVDRS
                     LQHIRDGGAKAGLALNPATPIEVLDYVLWRLDWVLVMSVNPGFGGQRFIAESLRKIAA
                     VRRMIEASGREIRLAVDGGVDVANIAEIARAGADTFVSGSAIFGSGDYAATIRRMRSE
                     LAAVA"
     gene            complement(2421..3068)
                     /gene="coq7"
                     /locus_tag="VFX38_06595"
     CDS             complement(2421..3068)
                     /gene="coq7"
                     /locus_tag="VFX38_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HEX5314553.1"
                     /translation="MSTRGTTPADRLLAAADRALRTVYGIPIGTGRPNPARAKPGARG
                     GDAPLRTRTGRLMRVNLAGEVAAQALYQGQSLTARSAAVAARMRRAAQEENDHLLWCR
                     ERLADLGAHPSRLNPLWYAGSLLIGAVAGAAGDARSLGFLEETERQVVAHLGRHLQRL
                     APGDEASRALLGQMRRDEARHGLHARTAGAKRPPPAIRLAMRLASRVMTGAAYWI"
BASE COUNT          492 a         1066 c         1070 g          615 t
ORIGIN      
        1 tttccccgcc gctcaaggtc ctgtatgcgg gccgtggcgg gcggcaaaag cgcgtgtggc
       61 tgctcaatcg catcgttgag tattttgtcc cgaccggcct tccgatcgag tttcattttg
      121 ctggaacgat ggctgacgag ctttcgccaa cggcgcgcga tgccgcgatc gtgcacggcg
      181 agatcggcaa tcgcacagaa atgcaggcgc tctatgctgc ctcgcatgtc gtcattctcg
      241 tctccggctt cgagggcttc ccgatggtgg tcaaggaagg catggcatcg agctgcgtcc
      301 cggtcgtcac cgccctaccg ggatatcaaa tgcacctcac ggatcacgag aacgccctgt
      361 tgatcgaggc ggtggacgac gaggtcgagg tcgtgcgact tgccatcgcg aggctcgaag
      421 agttgatggc cgatcgcgct ctcgtcgcga aactctcgac caacgcgaaa cagtatgcgc
      481 gccgcaattt cggcatggaa gcattccgcg cgaagtaccg cgagctgctg ctcgccccgc
      541 tggcgcgctc cgatagaatg taatccgatc gaaaaactcc tttcgaggtg ccatcgcgtg
      601 acgaacgtgc cttccgacaa aaccttcgtt ttcgttgccg ggctccacca cagcggaacc
      661 tcgcttctgc atcgcctcct cggcgctcat cccgagatca gcgctttttc gggcaccggc
      721 gtgcccgagg acgagggcca gcacctgcag tcgctgattc cgcccgcgct tgcctacggg
      781 gggccggcgc gattcggttt cgacccgcgg gcccgtctcg acgagaccag cccgcttgcg
      841 acgccgctgg gcgccgagaa gctgtacggc gagtgggcgc cgcactggga tctcgaaaag
      901 cgtcttttga tcgagaagtc gccgccgact ctcgtgcgtt tgcgtttttt tcaggcgctc
      961 tttccccatt cagtgtttgt agtcattctg cgccatccga ttgcgctcgc cgtcgccacg
     1021 cgaaggggct tcgtgcgcta taccttgcgc aacagctttt cggtcggccg tcgcggggga
     1081 aagtcggggc ccgcggcgtc ggcgcccttg catctgcgcg accttgccaa gctgcggctg
     1141 cgtcgggatg tcgaacatac cctcatctgc tacgagcagg cgttccgcga tctcgggcac
     1201 atcgatcgtg ccttcgtgtt gcgctacgaa gatttcgtcg ccgatccgaa cgccatgttg
     1261 cggcgcgtct tccggtttct gggagttgcg ccggtcgaag ccgccgaggc cgtgcgtcgc
     1321 gatgtgaacg cccgctatgt ccatgcctgg cgtagagggc ttctcgcgcc gctgcgcgcg
     1381 cgaattgcag cccgcttcga ggagcgggcg agggcggtgg gttacagtct cgtcgagccc
     1441 gcccggcagg tgccgctttc cgtttcgggt tccgacgtca ccgttgccga ggggccggcg
     1501 gccggtagct gagcgggcat gccgaacgcc tgccaaaccg ggatcgtcgc gtcctcccgt
     1561 acactgtagt ttcctcgcgc ccggagcctc ccgtgccgga attttggatc agtccctcgg
     1621 tgctttccgc cgatctggcg cgtcttggcg aggagtcggc cgcggtgctg cgcgcgggcg
     1681 gcgagcgcat tcatttcgat gtcatggaca atcactacgt cccgaacctg accttcgggc
     1741 cgtcggtgct gaaggcgctg cgcgcctacg ggatcgaggc gcccatcgat gctcacctga
     1801 tggtcacgcc gaccgatcgg ctgatccgcg attttctgga tgcgggcgcg gcgaccattc
     1861 tcattcatcc ggaggcgacc gagcatgtcg accgcagcct ccagcacatc cgcgacggcg
     1921 gcgcgaaggc cggcctcgcg ctcaatcccg cgactccgat cgaagttctc gactacgtgc
     1981 tctggcggct cgactgggtc ctcgtcatgt cggtgaatcc gggattcggc ggccagcgct
     2041 tcatcgccga gagcctgcgc aagatcgccg cggtgcgccg catgatcgag gcgagcgggc
     2101 gcgaaatccg gctcgcggtt gacggcggcg tggacgtggc aaacatcgcg gaaatcgccc
     2161 gcgccggcgc cgataccttt gtcagcggct cggcgatctt cggcagcggc gactacgccg
     2221 cgaccatccg gcgcatgcgc agcgaattgg ccgcggtcgc ttaaccagcg gcgatcgact
     2281 cggtaaacgg ggtaggttgg gctgaacgca gtaaagcccg acatcatggc gcctcacgtt
     2341 cacgtcaggt ttgcctgttg ggcttcgttt tgctcagccc aacctacatg ctgaatgctc
     2401 aagacacctg cgccagcccg tcagatccag taggccgcgc cggtcatcac gcgcgaggcg
     2461 agcctcatcg cgaggcgaat cgccggcggt ggtcgcttcg cgccggcggt gcgcgcgtgc
     2521 aagccgtgcc gcgcctcgtc gcggcgcatc tggccgagca gcgcccggct cgcctcgtcc
     2581 ccgggtgcga gccgctggag atgacggccg agatgcgcga cgacctggcg ttcggtttcc
     2641 tcgagaaaac cgagactgcg ggcatcgccg gcggcgcccg caaccgcgcc gatcagaagc
     2701 gagcctgcat accagagcgg attgaggcgg ctcggatgcg cgcccagatc cgcaagacgc
     2761 tcgcggcacc agagcaggtg atcgttttcc tcctgcgccg cccggcgcat tcttgcggcg
     2821 accgcagcgc tcctcgccgt caggctctgc ccctggtaca gcgcctgcgc ggcgacctcg
     2881 ccggcgagat tgacgcgcat gaggcggccg gtgcgagtgc gtagcggcgc atcgccgccg
     2941 cgcgcgcccg gcttcgcgcg agcgggattg ggtcgccccg ttccgatcgg gatcccgtac
     3001 accgtacgaa gcgcgcggtc ggcggcggcg agcagccgat ccgccggggt ggttccgcga
     3061 gtactcatcg cgaaagtcta gcggaggctc ggcttgccgg gcaacgcggc gggctgcgct
     3121 gttgcaaaca acgttcggga tccgttacga ctctgtgacg ggaatcgggg ttttacgccg
     3181 ggtgttgttg gtatcataag cgccgtgaaa ctttccgatg atcgtcgcag atgattgccg
     3241 cgg
//
LOCUS       DASUJG010000306         2260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1555605, whole genome shotgun sequence.
ACCESSION   DASUJG010000306 DASUJG010000000
VERSION     DASUJG010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555605"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1249)
                     /gene="ggt"
                     /locus_tag="VFX38_06600"
     CDS             complement(<1..1249)
                     /gene="ggt"
                     /locus_tag="VFX38_06600"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX5314554.1"
                     /translation="MSSRAASILRPVVAFLVLLFVAAGGVNAATPAPVLASHGMVATA
                     EHLATEVGVDVLRHGGNAVDAAVAVGYALAVVDPCCGNLGGGGFMTLHLADGKNLFLN
                     FREKAPLAASANMYLGPHGEVEQGKSLWSWQAIGVPGTVMGLDAALSRYGTMSRAEVM
                     APAIRLAREGYVLEAGDAALFAAGAERLARDPTTARIFLPHGHVPRAGERFQQPELAR
                     TLELISRDGDKAFYEGPIAKEIVAASKANGGILSRKDFHAYTVEWEQPVACEYRGYEI
                     VSAPPPSSGGVTLCEILNVLAGWPDFAGYEWHSVPAVHDMVEAMRFAYADRNGYLGDP
                     DFVNVPVAKLLSARHAAAIRARIPADRAVPSSSIHVDLAPAEGTHTTHYSIVDAKGNA
                     VGVTYTLNSWFGTGFVASGTGFLL"
     gene            1352..>2260
                     /locus_tag="VFX38_06605"
     CDS             1352..>2260
                     /locus_tag="VFX38_06605"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5314555.1"
                     /translation="MTRAIEICAATKRFGSHTAVDELSLEVPQAVIYGFLGPNGAGKT
                     TTLRMITGILDADSGSVTVLDGSQPAAARNRIGYLPEEKGLYKDMQVADIVAYFGELK
                     GLSHREALARAKDRLAAAGLGDWLNSKCNALSKGMAQKVQVIATLLHDPELVVLDEPF
                     SGLDPVNSEMVLELIKRYKREGRTVIFSTHIVEHAEQICDSIVIIDRGRRKVAGTLAD
                     VKASADRTVLLDYEGNAGNLALLPGVLTADDSGQHAELKLAADADDRALLETLAARVR
                     IRRFDTREASLKQVFLGAVEETREARS"
BASE COUNT          407 a          757 c          740 g          356 t
ORIGIN      
        1 tgagaagaaa gcccgtcccg gaagcgacga agccggtgcc gaaccacgaa ttgagcgtgt
       61 aggtgacgcc gacggcgttg cccttcgcgt ccacgatcga gtagtgcgtg gtatgcgtgc
      121 cttccgccgg cgcgagatcg acatggatcg aactcgaagg caccgcgcga tcggcgggga
      181 tgcgggcgcg gatcgcggcc gcgtggcgcg ccgacagaag cttcgcgacc ggaacattca
      241 caaagtcggg atcgccgagg tagccgttgc gatcggcgta ggcgaagcgc atcgcctcca
      301 ccatgtcgtg caccgcgggc accgaatgcc attcgtaacc cgcgaagtcg ggccagccgg
      361 cgagcacgtt cagaatctcg cacagggtga cgccgcccga actcggcggc ggcgcggaca
      421 caatttcgta gccgcgatat tcgcaggcga cgggctgctc ccactcgacc gtgtaggcgt
      481 gaaaatcctt ccggctcaat atcccgccgt tcgccttcga agccgcgacg atctccttcg
      541 cgatcggtcc ttcgtagaag gccttgtcgc cgtcgcggga aatgagttcg agcgtgcggg
      601 cgagttccgg ttgctggaag cgttcgcccg cccgcggcac gtggccgtgc ggaagaaaga
      661 tccgcgccgt ggtcggatcg cgagcgaggc gctcggcgcc ggcggcgaag agtgcggcat
      721 cgcccgcttc gagcacgtag ccctcgcgtg cgagccggat cgcgggcgcc atgacttcgg
      781 cgcggctcat ggttccgtaa cgcgagagcg cggcatcgag tcccatcacc gtgcccggca
      841 cgccgatcgc ctgccaactc cagaggctct tgccctgctc gacctcgccg tgggggccga
      901 gatacatgtt tgcgctcgcc gcgagcggcg ccttctcgcg gaagttgaga aagagattct
      961 tgccgtccgc aagatgaagc gtcatgaagc cgccgccgcc gagattgccg cagcagggat
     1021 cgacgacggc gagcgcatag ccgacggcga ccgcggcatc gacggcgttg ccgccgtgac
     1081 gcagcacatc cacgcccacc tcggtcgcga gatgctcggc ggtcgcgacc atcccgtggg
     1141 atgcaagcac gggcgcgggt gtcgcggcgt tcacgccgcc ggccgcgacg aaaagcagaa
     1201 caaggaatgc gacgacgggg cgcaggatgc tggcggcgcg gctactcacg gttaccagtc
     1261 taacggacgc cgagcgcttt gggggcaatg ccgccttccg gtcgcgaccc cgcttcgtta
     1321 taattcgcgc acttcgccgc ggtcccgcag catgacccgc gccatcgaaa tttgcgccgc
     1381 gaccaagcgt ttcggttccc acacagcggt cgacgagctt tcgctcgagg taccgcaagc
     1441 cgtcatttac ggttttctcg gtccgaacgg cgccggcaag accacgaccc tgcgcatgat
     1501 caccggcatt ctcgacgccg attccgggag cgtgacggtg ctagacggga gccagcccgc
     1561 cgcggcgcgc aaccgcatcg gctatctccc ggaagaaaag ggtctctaca aggacatgca
     1621 ggtcgccgac atcgtcgcct acttcggcga actcaagggg ctttcgcatc gcgaggcgct
     1681 cgcgcgggcg aaagatcgcc tcgcggctgc cggactcggc gactggctga actccaagtg
     1741 caatgcgcta tccaagggca tggcgcagaa ggtgcaggta atcgcgacgc tgctgcacga
     1801 cccggagctg gtcgtgctcg acgaaccctt ctccgggctc gatccggtga actccgaaat
     1861 ggtgctcgaa ctcatcaagc gctacaaacg cgagggacgc acggtgatct tctcgaccca
     1921 tatcgtcgag cacgccgagc agatctgcga ctccatcgtc atcatcgacc gcggccggcg
     1981 caaggtcgcg ggcacgctcg ccgacgtcaa ggcttcggcg gatcgcaccg tgctgctcga
     2041 ctacgagggc aatgcgggga atctcgcgct tcttccaggc gtgctcacgg ccgacgattc
     2101 cggccagcac gccgaactca agctcgccgc cgacgccgac gaccgcgcgc ttctcgaaac
     2161 tctcgcggca cgagtgcgca tccgccgctt cgacacccgc gaggcctcgc tcaagcaggt
     2221 ctttctcggc gcggtggagg aaacccgtga agctagatcg
//
LOCUS       DASUJG010000307         2875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_576083, whole genome shotgun sequence.
ACCESSION   DASUJG010000307 DASUJG010000000
VERSION     DASUJG010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576083"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VFX38_06610"
     CDS             complement(<1..587)
                     /locus_tag="VFX38_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5314556.1"
                     /translation="MRRSNFEVPALVLAVAAISFPAGAAESPQVDAQQVAPQLWYLHD
                     QGGNVTALIGPDGTLLVDAEFAPDVPALLAKIKALGGTAPRFVINTHYHEDHTGGNAA
                     MRAAGAAIIAQENVRARLLQVQRAPLDGSLPAPVPAADLPMITFERSLTLHFDGETVQ
                     VKHLPPAHTDGDSVVRFEEANVIATGDLYFNGMYP"
     gene            complement(616..1488)
                     /locus_tag="VFX38_06615"
     CDS             complement(616..1488)
                     /locus_tag="VFX38_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018620301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEX5314557.1"
                     /translation="MSQKSSPEKIPGESEVEAEAQYLEQHERREAARRTAPRAAIVYE
                     AVRREGEQELARPIAALAWSGLAAGLSMGFSFLSQSLLASWLPQAHWSIVVSGLGYSV
                     GFLMVILGRQQLFTENTLTPILVFLRKPDAVTAGRTLRLWVVVLIANTLGAFLFALLI
                     AKTNLLVPQVHAAMNEVSSQVFAAFPLTLLRGIFAGWLIAMIIWLLPFAETARVGIII
                     LITYVIALGHFAHIIAGSVDAFYAALSGNVPWGAFFTEFYVPTVLGNIIGGVSLVAAI
                     NYAQVETLEQRNSD"
     gene            1665..2135
                     /locus_tag="VFX38_06620"
     CDS             1665..2135
                     /locus_tag="VFX38_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5314558.1"
                     /translation="MTETKWKIRDAIPEDAATVTAFNLACAEESEGMKLDTGRLRAGV
                     EAVLADAGRGRYFLAEDERGVAGQVMITFEWSDWRNAWVWWLQSVYVRPDVRRAGVFS
                     ALMQHVETEARRAAIPLVRLYVEHDNEVAERTYRANGFEAAPYRLLRKALGSDA"
     gene            2128..2571
                     /locus_tag="VFX38_06625"
     CDS             2128..2571
                     /locus_tag="VFX38_06625"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012938029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX5314559.1"
                     /translation="MLETGARAPDFTLPDQNGDAVHLAAVLASGPVLLYFYPADFTPG
                     CTREACAFRDLYPTLAAAKLTVLGVSPQDSASHRRFREKYHLPFSLLADPERTVIRAY
                     QCEGPLGLLRRTSYRIGPDGTIRRALRADLRIGRHRAFAAEIAEP"
BASE COUNT          506 a          947 c          932 g          490 t
ORIGIN      
        1 tagggataca tgccgttgaa atagagatcg ccggtcgcga tcacgttcgc ttcctcgaag
       61 cgcacgacgg aatcgccgtc ggtgtgcgcc ggcggcagat gtttcacctg cacggtctcg
      121 ccatcgaaat gaagtgtcag ggatcgctcg aaggtgatca tcggaagatc ggccgcgggc
      181 accggcgccg gcagcgaacc gtcgagcggc gcgcgctgca cctggagaag ccgcgcgcgt
      241 acgttctcct gcgcgatgat cgcggcaccc gcggcacgca tcgcagcgtt cccgccggtg
      301 tgatcttcgt ggtagtgcgt gttgatcacg aagcgcggcg cagtcccgcc gagcgccttg
      361 atctttgcga gaagcgccgg aacgtcgggg gcgaactccg catccacgag aagcgtgccg
      421 tccggtccga tgagcgcggt gacgttgccg ccctgatcgt gcagatacca gagctgcggc
      481 gcgacctgct gcgcgtcgac ttgcgggctt tccgccgcgc cggcggggaa agagatggcc
      541 gcgacggcga gaacgagtgc cgggacttcg aagttcgatc gtctcatggt ctttctccgt
      601 gccgggttgc ggtgatcaat ccgaattgcg ttgctcgagc gtctcgacct gggcgtagtt
      661 gatcgccgcg accagggaga cgcctccgat gatgttgccg agcaccgtcg gcacatagaa
      721 ctccgtgaaa aatgctcccc aggggacgtt cccgctcaag gccgcataga acgcatccac
      781 ggaaccggcg atgatgtgcg caaaatgtcc gagcgcgatg acataggtga tgaggatgat
      841 aatccccacg cgggcggttt ccgcgaaggg cagaagccag atgatcatcg cgatcagcca
      901 gcccgcaaag atcccccgaa gcagagtcag cggaaaggcg gcgaagacct ggctgctcac
      961 ctcgttcatg gctgcatgca cctgcggaac gagcagattc gtcttcgcga tcaggagcgc
     1021 gaacaggaag gcgccgagcg tattggcgat cagcaccacg acccagagac gcagcgtccg
     1081 ccccgcggtc acggcatccg gtttgcgcag gaacaccagg atgggagtca gcgtattttc
     1141 ggtgaagagc tgctgcctgc cgagaatcac catcaggaat cccacgctgt atccgaggcc
     1201 ggaaaccacg atgctccagt gggcctgcgg cagccagctc gcaagcagcg actgggacag
     1261 aaacgaaaac cccatcgaaa gccccgccgc gagtcccgac caggcgagcg ccgcaatcgg
     1321 ccgggcgagt tcctgttccc cttcgcggcg cacggcctcg tatacgatcg ccgcccgcgg
     1381 tgcggtacgc cgcgccgctt cgcggcgctc gtgctgctcc aggtactgcg cttcggcctc
     1441 gacctcggat tcgcccggaa tcttttccgg ggaagatttt tgcgacatgc acgcggctcc
     1501 cgcgctttgc gtcatgatag cagcgcgcac cttggaaatc atcgcgacag atagtgtgtg
     1561 cgccggcgca ccaagaccgg tgcaggcgca atggattcgc catcgccgca acactcgcgc
     1621 ccgggtttca ttacactgat gccagtctgc ccgaggacgc gagaatgacc gaaacgaagt
     1681 ggaagattcg cgacgcgatt ccggaggacg cggcgacggt cacggccttc aatctcgcct
     1741 gcgccgagga gagtgaaggg atgaaactcg acaccgggcg cctgcgtgcc ggagtcgagg
     1801 cggtgctcgc cgatgcgggc cgcgggcgct acttcctcgc cgaagacgag cggggcgtgg
     1861 cggggcaggt catgatcacc ttcgagtgga gcgactggcg caatgcctgg gtctggtggc
     1921 tgcaaagcgt ctatgtgcgc ccggatgtgc ggcgcgccgg cgtcttctcg gccttgatgc
     1981 aacacgtgga gaccgaggcc cggcgtgccg cgattccgct cgtccgcctg tatgtcgagc
     2041 acgacaacga ggtcgccgag cgcacgtatc gcgccaacgg cttcgaagcg gcgccctacc
     2101 ggctgctgcg aaaggcactc ggctccgatg cttgaaaccg gcgctcgcgc ccccgacttc
     2161 acgctccccg accagaacgg cgacgccgtt catcttgcgg cagtgctcgc ctcgggaccc
     2221 gtgcttctct atttctaccc ggccgatttc acgcccggct gcacccgcga ggcttgcgcg
     2281 ttccgcgatc tgtatccgac gctcgcggcc gcgaagctga cggtgctcgg cgtaagtccg
     2341 caggactccg cgagccaccg tcgcttccgc gagaagtatc acctgccctt ctcgctcctc
     2401 gccgatcccg aacgcacggt gattcgcgcc taccaatgcg agggaccgct gggactcctg
     2461 cggcggacta gctaccggat cggccccgac gggacaatcc gccgggcgct gcgcgccgat
     2521 cttcgcatcg ggcggcaccg ggcgttcgcc gcggagattg ccgagccctg agccaaggcg
     2581 cgagcccggc cgaaggctcg attcgcaggg cgccttaccc gattccgcag gcgcagcgta
     2641 aaggcttgcc ctccatttgg gaccgtgtta tagttcgcta caacagcgta tcggacagga
     2701 ctcgtgtcgc ggcccccggc taccgatata atgcccgctt ttcgcgcccg ccccgggtgc
     2761 tcggaagctt cgcggaggcc atccaaggcc ctcgccggcg gttcggccgc agcactttcg
     2821 gcggactcgc ctgcaccggg ttcgtgtcgc agcgatattc cctgtttcct gattc
//
LOCUS       DASUJG010000308         2320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1311238, whole genome shotgun sequence.
ACCESSION   DASUJG010000308 DASUJG010000000
VERSION     DASUJG010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311238"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VFX38_06630"
     CDS             complement(<1..435)
                     /locus_tag="VFX38_06630"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEX5314560.1"
                     /translation="MNEFAETLPREPESALRLSFEFFPPKTPAMDARLWDTVERLTPF
                     RPDFVSVTYGAGGSTRDRTHATVERIRRDTPLEPAAHLTCVGATRGEIDEVVDRYVTT
                     GASRSPMMPPARWPDEPGQASGRQPPARLLRVLSAEDRADGGD"
     regulatory      complement(539..615)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            complement(699..1820)
                     /locus_tag="VFX38_06635"
     CDS             complement(699..1820)
                     /locus_tag="VFX38_06635"
                     /inference="COORDINATES: protein motif:HMM:NF020874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HEX5314561.1"
                     /translation="MREAISVAICGTRGIPNNYGGFEQFAQHLAVELVARGYDVIVYN
                     PHFHALSDGSFHGAKIVCKWCPESRLGPFAHYVYDYLCLRDAVRRRTSIILECGCTSA
                     GPGLLFLRKRGSTVVLNTDGVEWKRAKWGRVAKMLIRLSEKAAIKRAEHLVADNAGIA
                     AYLSQEYDEDSTVIPYGTTIPESVDASLLDSYDLAQYGYFLAVARLEPENNIEAILRG
                     FLASGSEKFFCIVGNHDTRYGRHLRTTFRDERIKYLGGIYRQPVLHALRHFSALYFHG
                     HSVGGTNPSLLDAMADGALICAHDNVFNRAVLGDDALYFSAMEEVAESIRREEENLKL
                     RPLIVENNLAKVRSQYSWQTISDAYEALFLRISAKRSAH"
     gene            2051..>2320
                     /locus_tag="VFX38_06640"
     CDS             2051..>2320
                     /locus_tag="VFX38_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEX5314562.1"
                     /translation="MYRSQLASRATTFFAAGLLAIGVIAARPTGAAAATAPAAATAAS
                     PASMAALRALAEKGNAEAQYALGVSYGRGQRVKQDYAEAAQWFTKA"
BASE COUNT          432 a          737 c          729 g          422 t
ORIGIN      
        1 gtctcctcca tctgctcggt cttcggcgga aagaactcga aggagacgcg caggcggctg
       61 tcgcccggat gcttgacctg gctcatcagg ccacctcgcg ggcggcatca tcggcgacct
      121 ggatgcgccg gtcgtgacat agcgatcgac gacctcgtcg atctcgccgc gcgtcgcccc
      181 gacgcaggtg agatgcgccg ccggttcgag cggagtgtcg cgacggattc gctccaccgt
      241 ggcatgcgtg cgatcgcgcg tcgagccgcc ggcgccgtag gtgaccgaga cgaagtccgg
      301 acgaaacggt gtcaggcgct cgaccgtatc ccacagtcgc gcgtccatcg ccggggtttt
      361 cggcggaaag aattcgaagg aaagccgcaa cgcgctttcc ggttcgcgcg gcaaggtttc
      421 tgcaaattcg ttcatggcat ccgttcccga tgccgcagta tttcgactcc gccccacgat
      481 gcatccacgg gttccggcgg tcgcgggcaa caaaaaaccc gcccggctgc acgccaaaga
      541 gcggggtttc tccgtctctt tagcggcatt tctaacgcgc ccgcaagctg acttcaaatc
      601 ggcgcaaaga caatatagca caggagcccg gcctattacc gcccggcctt tccgccgcgg
      661 acgacagtca aatcaggcga gtcggcggga aggacccctt agtgggccga tcgcttcgcg
      721 gagatgcgaa ggaacaacgc ctcgtaggcg tcgctgatgg tttgccacga atattggctg
      781 cgcaccttgg cgaggttgtt ttccactata agcggccgaa gtttcaggtt ttcctcctcc
      841 cggcggatgg attcggcaac ctcttccatt gcggaaaagt agagcgcatc gtcgcccagg
      901 acggcacgat tgaatacatt gtcgtgcgcg cagatcagcg caccgtcggc catcgcgtcg
      961 agaagcgagg gattcgtccc tcccacggaa tgcccgtgga aatacagggc ggagaaatgg
     1021 cgcagggcgt gcagcaccgg ttgccggtag atgccgccga gatatttgat gcgctcgtcg
     1081 cgaaacgtcg tgcgcagatg gcgaccgtag cgcgtgtcgt ggttgccgac gatacagaag
     1141 aacttctcgc tccccgaagc gagaaagcct cgcagtatcg cctcgatgtt gttttccggt
     1201 tcgagccgtg cgacagcgag aaaatatccg tattgcgcga gatcgtagct gtccagcagg
     1261 gaggcatcga ccgactccgg tatcgtcgtt ccatacggaa ttaccgtcga gtcctcgtcg
     1321 tactcctggc tcaggtaggc cgcgatcccg gcattgtcgg caacgagatg ttcggccctc
     1381 ttgatggcgg ccttttccga gagacgaatc agcatctttg cgacccgacc ccatttcgcg
     1441 cgcttccact ccacgccatc ggtgttgagt actaccgtgc tgccgcgctt gcgcaggaaa
     1501 agcaggccgg gcccggcact cgtgcaaccg cattccagaa tgatggacgt gcgtcgccga
     1561 actgcatcgc gaaggcaaag gtaatcgtag acatagtggg cgaaagggcc gagacgggac
     1621 tccgggcacc acttgcacac gattttcgcc ccgtgaaagg agccgtcgct cagtgcatgg
     1681 aaatgggggt tgtagacgat gacatcgtag ccgcgggcga cgagttcgac cgcgagatgc
     1741 tgggcgaatt gctcgaagcc gccatagttg ttggggatcc cgcgagtgcc gcagatcgcc
     1801 acgctgatcg cctcgcgcat tcagtgcttc ttcgacgggt atgccggcga tcatacaatg
     1861 aatgtgccgc cgctgcagcc tgcgatcccc gaaccctccg ccccggcgac gtggtgcggc
     1921 ctcccgggtc agccgctcat tgctcccgcc tcacgggggg tacgaagcgc cgagcggagc
     1981 agctcgcgcc cgcttctccc gccgcacggt tcggctatag tggaaatcgc ctcccgcatc
     2041 cggaccacac atgtatcgct cgcagctcgc gtcacgggcc acaacctttt tcgccgcagg
     2101 attgctggcg atcggggtca tcgcggcgcg gccgactggg gccgctgccg caaccgctcc
     2161 cgcggcggca acggccgcga gccccgccag catggcggct ctccgcgcgc tggccgaaaa
     2221 gggcaatgcc gaagctcagt acgccctcgg ggtgtcctat ggccgcggcc agcgagtaaa
     2281 gcaggattac gccgaggccg cgcagtggtt cacgaaagcg
//
LOCUS       DASUJG010000309         2301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1433358, whole genome shotgun sequence.
ACCESSION   DASUJG010000309 DASUJG010000000
VERSION     DASUJG010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433358"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VFX38_06645"
     CDS             <1..185
                     /locus_tag="VFX38_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314563.1"
                     /translation="GDWPRTSGSLGRQSGLGQPLGAPDLPDVLIFRNRVKPVIEKDFA
                     FPETEIRCMYPPSRLR"
     gene            complement(778..862)
                     /locus_tag="VFX38_06650"
     tRNA            complement(778..862)
                     /locus_tag="VFX38_06650"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(826..828),aa:Leu,seq:caa)
     gene            1042..1581
                     /locus_tag="VFX38_06655"
     CDS             1042..1581
                     /locus_tag="VFX38_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEX5314564.1"
                     /translation="MTTQTSALPHTRHSEAAAATRAALAVMAIAAATLAGAWFFQLVL
                     EILPCPLCLEQRYTYYFAVPFAGVVAFAAARGAPRGVVLAGLAILALAALGNAILGGY
                     HAGVEWKFWPGPTDCTGTGFNPGKAGSLFDSLDKVKVVRCDEVQFRFLGISLAGYNVL
                     ISLLMAAIAAWGFVRTVRR"
     gene            complement(1692..>2301)
                     /locus_tag="VFX38_06660"
     CDS             complement(1692..>2301)
                     /locus_tag="VFX38_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEX5314565.1"
                     /translation="MRVIILTAALPMVLALTGVAPANSAGLTLPVASPMHLVALLAAS
                     LAMSLILRMLKFPASWMFGAMIASSALHGTGLVDGGVPPWARSVALVGIGALIGSRFA
                     RMKPKVLAGHINAALGSFAVAIAVSGLFVAIIAFATNVRLSDVVVAFAPGAMDAMLAL
                     ALTLHIDPIFVGAHHLSRFVFVTIATPGIVHLFGRTQDDVDD"
BASE COUNT          404 a          711 c          766 g          420 t
ORIGIN      
        1 ttggcgactg gccgcgaact tcgggaagtc tcggtcggca gagcgggctc ggtcagccgc
       61 ttggcgcccc tgatttgccc gacgtgctga ttttcagaaa tcgtgtcaag cccgtcattg
      121 aaaaagattt cgctttccca gaaacagaaa tcaggtgtat gtacccgccg tcccgcctcc
      181 gctgagaggg gcgtttcgcg atcgtcacga acgttgggtg cgggatgcga tggacgtgtt
      241 ggcgcgcgca aagacgagcg gcgcaaatgc ggacggcgaa atcgtgtggt cctggcctcc
      301 cgacgctgag gtcaagcttt tcgcgaggcg ttcgcgaggg cgacgggggc tatcaagccc
      361 ggcaccccgg ggagatcacg tataaggcgt aacaccatcg cgcagggaag gccggatcgt
      421 tcggcgtacc tgtagtgact tgcttgcgtg cttttttaca cttgcacgcg aggctgcggg
      481 tgcgatcgtc gcgccggctt tccctgcgcc ctccgtcttt cgaggacgct gttcttgcaa
      541 aatccgggcg aaacgcgccg cgggaatgcg gacgtgtgtg tcccgtcatt gcgagcgaag
      601 cgaagcaatc cagaccgtct ccggagagac aatgtggatt gtttcgctgc gctcgcaatg
      661 acggagaagc tgtttgacaa tcgaatctgg aaattgcggc gaagtcgcac agcttcaccc
      721 ggggcagtcc gtcttcgctc tcgctgcgct cggctacgcc ggacacgctt cgaagcttgg
      781 tgcccctggc cggaatcgaa ccagcactcc ttgcggaact cgattttgag tcgagcgcgt
      841 ctaccagttc cgccacaggg gcattcgcgt gtccctgggg ttttatgtcc catcggcagc
      901 gaagccggcg gaatatagct ggcggcaacg gagggtcaac ccgcgcggat gtgatttcga
      961 gccggctcga caggccatgc gctgcgggat acgaagttta ccgacctgtt tactccggaa
     1021 ttgggaccag ggaagacgga cgtgacgacc caaacatcgg ctcttccgca caccaggcac
     1081 agcgaggcgg cggctgcaac gcgcgcggcg cttgcggtca tggcgatcgc ggcggcaacg
     1141 ctggcgggcg cctggttctt ccagctcgtg ctggagatcc tgccctgtcc gctctgtctc
     1201 gagcagcgct atacctatta tttcgcggtg cccttcgcgg gcgtggtggc gttcgcggcc
     1261 gccaggggtg cgccgcgtgg tgtggtgctc gcgggccttg ccatccttgc gctcgccgcg
     1321 ctcggcaatg cgatcctcgg cggctatcat gccggcgtcg aatggaaatt ctggcctgga
     1381 ccaaccgatt gcaccgggac cggcttcaat ccgggcaagg ccggcagcct gttcgacagt
     1441 ctcgacaagg tgaaggtggt ccgctgcgac gaggtgcaat tccgcttcct cggtatttcg
     1501 ctcgccggct acaacgtgct gatctcgctt ctgatggccg cgatcgcggc gtggggattt
     1561 gtgaggacgg tgaggcgtta gactgtaggg tgggcgaagg cgcgaacgcg ccgtgcccac
     1621 catctatcgg cagcgtgatt cgtgaatggt gggcacgctg cgctttgccc gccctaccgt
     1681 ctcactaccg tctaatcgtc cacgtcgtcc tgtgtgcgtc cgaacaggtg cacgatgcca
     1741 ggcgtcgcga tggtcacgaa cacgaagcgc gacagatgat gcgcgccgac gaagatcggg
     1801 tcgatatgca gcgtcaacgc cagcgccagc atggcgtcca tcgcgccggg ggcgaaggcg
     1861 accacgacgt ccgacaggcg cacattggtc gcgaacgcga tgatcgcgac gaagaggccg
     1921 gacacggcga tagcgaccgc gaacgagccg agcgccgcgt tgatatggcc ggcgagcacc
     1981 ttcggcttca tcctggcgaa gcggctgccg atcagagctc cgatgccgac cagcgccacg
     2041 ctgcgcgccc agggcggcac accgccgtcg acgaggcccg tgccatgcag cgcgctggaa
     2101 gcgatcatcg ccccgaacat ccagctcgcc ggaaatttca gcatgcgcag gatcagcgac
     2161 atcgcgagcg acgccgcgag cagtgcgacg agatgcatcg gcgaggcgac cggcagcgtc
     2221 agccctgcgc tgttggcggg cgcgaccccc gtcagcgcca gcaccatcgg cagcgcggcc
     2281 gtgaggatga tgacccgcat c
//
LOCUS       DASUJG010000310         4317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1066592, whole genome shotgun sequence.
ACCESSION   DASUJG010000310 DASUJG010000000
VERSION     DASUJG010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066592"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            361..1791
                     /locus_tag="VFX38_06665"
     CDS             361..1791
                     /locus_tag="VFX38_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5314566.1"
                     /translation="MDQLDALRAVLGAAQVLTGADAEPYALDWRERYRGRALAVARPG
                     SANEVAAVVRLCAQANIAIVPQGGNTGLCGGATPDDTGRAMILSLQRLRRIRGIDTDN
                     DTMEVEAGCVLQAVQEAARAAGRLFPLSLASEGSCTIGGNLATNAGGTEVLRYGNMRE
                     LALGLEFVTAQGDIWHGLRGLRKDNSGYDLRDLFIGSEGTLGVITAATLKLHPLPAAR
                     CTALLALDSLASAIAMLARARSGFGASLSGFELISGECLQLVAHCFPQQRLPFGGPSA
                     TMPWYVLLELSDSESEAHARQRFETVVEAAIEAGCVKDAVIAESIAQSRELWHLRESV
                     PLAEKDSGKSVKHDISLPLSRMAEFVPATNAALQAAFPGIRHVIFGHLGDGNLHYNVA
                     PGPDWTEEQLLAREDDIHALVHDRVRAAGGSISAEHGIGQLKRDMLPRYKDPVELELM
                     RRIKAVLDPQGIMNPGKLLQTRPQRA"
     gene            2007..3020
                     /locus_tag="VFX38_06670"
     CDS             2007..3020
                     /locus_tag="VFX38_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008212905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314567.1"
                     /translation="MLAALLLALVQAAAVLATAAVAVPVQYDAARIIARPRTADGQTL
                     NLWVDTGGGGGAGMYLLTDEAVRRLHLDTGHIEADGQSIPVADPPKFAPGAAIPPPAG
                     KYDKAIVVPANGFRGPGDSTRYDGMLGAGYLPGNPATHARIWTFDYPGGRLTLEGANW
                     QPSANAHATRLHFPLDKKGRLEAGFARIVVRVDGQPLSLLLDTGATAYPTPAALAAEG
                     GRATVRATSFITTTQLESWHKAHPDWRVIADADRMKIKGKPMRAIEVPAVEIAGWLTG
                     PVWFTERPDTNFHDFMSSMMDAQVEGALGGNAFEHFAMSVDYGRAKAYFRCVSGCVPA
                     SGP"
     gene            3178..4263
                     /locus_tag="VFX38_06675"
     CDS             3178..4263
                     /locus_tag="VFX38_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016901988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5694 domain-containing protein"
                     /protein_id="HEX5314568.1"
                     /translation="MKFRFAMYAMLAPLIFSETAQAQVSLSALADHMAGPPTQILVLG
                     TAHLSNAPKGFEPQSLQPLLARLAAYQPQIITIEAISGEGCALLAEYPTIYNPENVAP
                     FCADTTAAKAATGLDVPAAVVAADKALKDWPADPTPAQRRHLAALFLASGEPASALVQ
                     WLQLPKAERHKGDGLDDALVTLLKKKRASNNEDYQLAVPLAVKLGLQRVYPIDDHTGD
                     NIRITDQQTKGFVAAIRKAWDSTKSQMQPIEDREHDLLEHNHTLELYRYLNSPDVQQA
                     FIASDMGAALRDDSAQHYGQMYVTGWEARNLRMAANILATFREHPGARVLTLVGATHK
                     PWLDKILGQMPGVEIVDTEKMLGAAAQ"
BASE COUNT          773 a         1490 c         1381 g          673 t
ORIGIN      
        1 aagctgcggg ccgcgggccg cgccgagtcc ggctcgggac gatcgttcaa gctgcggcgg
       61 ccttgctccc gaaacgcgag gcgacgagca cggacgcgaa ttcgacacgg cgggccggcg
      121 gctgccgatt cgcggtggca aaaaaattcc gggtcaagag cacaaacctc ggagcaaacc
      181 gtgccgttac cggatatttt acgtcgcctt tcgcgatgga acgcgcacga gcctcccgcc
      241 gcgccccgcg atgctctgcc ggtcggcctg ccggactcgc ccgacagcgg ccgcgaagct
      301 cgcgccccgc gccgattaca ctaaacatct gctttcggcc cgagggaatt ttcccaaacc
      361 atggatcagc ttgatgcctt gcgggcggtg ctcggcgcag cgcaggttct gaccggcgcg
      421 gatgccgagc cctacgcgct ggactggcgc gagcgctatc gcgggcgggc gctcgccgtc
      481 gcgaggccgg gctccgccaa cgaggttgcg gccgtcgtgc ggctgtgcgc gcaggcgaac
      541 atcgcgatcg ttccgcaggg cgggaacacc ggcctgtgcg gcggcgccac gcccgacgat
      601 accggccgcg ccatgatcct gtccctgcag cgcctgcgcc gaatccgcgg catcgacacg
      661 gacaacgaca ccatggaagt cgaggccggc tgcgtgctgc aggcggtgca ggaagccgcg
      721 cgcgcggcgg gacgactctt tcctctgagc ctcgcctccg aagggagctg cacgatcggt
      781 ggcaatctcg cgaccaacgc gggcggcacc gaagtgctcc gctacgggaa catgcgcgaa
      841 ctcgcgctgg gactcgagtt cgtgaccgcg cagggcgaca tctggcacgg actgcgcggt
      901 ctgcgcaagg acaacagtgg ctacgatctg cgcgatctgt tcatcggcag cgaaggcacc
      961 ctcggcgtga tcacggcggc aacgctgaaa ctgcatccgc tgccggcggc tcgctgcacc
     1021 gcgctcctcg cactcgattc gctcgcctcg gcgatcgcga tgctcgcccg tgcccgcagc
     1081 ggcttcggcg cctcgttgag cggcttcgag ctgatctccg gcgaatgcct gcagttggtg
     1141 gcgcactgtt tcccgcaaca gcgtctgccc ttcggcggac cttcggcgac gatgccgtgg
     1201 tacgtgttgc tcgaactgtc ggacagcgaa tccgaggcgc atgcgcgcca acgcttcgag
     1261 acggtcgtcg aagcggccat cgaggcgggc tgcgtgaagg atgcggtgat tgcggaatcg
     1321 atcgcccaaa gccgcgaact gtggcatctg cgcgaaagcg ttccgctggc ggaaaaggat
     1381 tccggcaaga gcgtcaagca cgacatctcg cttccgctgt cgcgcatggc ggaattcgtg
     1441 cctgccacca atgcagcatt gcaggccgcg ttcccgggga tccggcacgt cattttcggc
     1501 catctcggcg acggcaacct gcattacaac gtcgcgcccg gcccggactg gacggaagaa
     1561 caattgttgg ctcgcgaaga cgacatccac gcgctggtgc acgatcgcgt ccgcgccgcg
     1621 ggcggttcca tcagcgccga gcacggcatc ggccaactca aacgcgacat gcttccgcgc
     1681 tacaaggatc cggtggagtt ggaattgatg cggcgcatca aggcagtcct cgatccgcag
     1741 ggcatcatga atcccggcaa gctcctgcaa acccgcccgc agcgcgcgta gcccgcgaga
     1801 gcgttgctct ggtatgcact gctcgtggcc ggcatggccc tgagtctgct tcgtcgcaag
     1861 ccgcggaacg acgcaaggta agctgtaggt tgggatgagt ccgcgaagcc caaatttccg
     1921 acgcggcgtt ccgaatcgcc ggccgccggc attcggacga gcggcgagcg gatatgatgg
     1981 cgatgtgtcc agccgaaccg ccgatcatgc ttgccgccct cctgctcgct ctcgtccagg
     2041 ccgccgccgt tctggcgacg gcggcagtcg ccgttccggt gcaatacgat gccgcccgca
     2101 ttatcgcccg gccccgcacc gccgacggac aaacgctgaa tctgtgggtg gacacgggtg
     2161 gcggcggggg cgccggaatg tatctgctca ccgatgaggc ggtgcgacga ctgcatctcg
     2221 acaccggcca tatcgaagcg gacggacaat ccatcccggt cgccgatccg ccgaagtttg
     2281 cacccggcgc cgccattccc ccaccggccg gcaaatacga caaggccatc gtcgttccgg
     2341 cgaacggctt ccgcggcccc ggcgattcca cccgctacga cggcatgctg ggcgcgggct
     2401 acctgccggg gaaccccgcg acgcacgctc gcatctggac cttcgattat cccggcggcc
     2461 gcctgacgct cgaaggcgcg aactggcagc catcggcgaa tgcgcatgcg acgcggctgc
     2521 attttccgct cgacaagaag ggccgtctcg aagcgggttt cgcgcgcatc gtcgtgcgcg
     2581 tggacggcca gccgctcagc ctgcttcttg acaccggcgc aacggcctat ccgacgcccg
     2641 ccgccctcgc ggcggaaggc ggccgcgcca ccgtgcgcgc gaccagtttc atcaccacca
     2701 cccaactcga aagctggcac aaggcgcatc ccgactggcg tgtgatcgcg gatgccgatc
     2761 gcatgaagat caagggcaag ccgatgcgcg ccatcgaagt gccggccgtg gaaatcgcgg
     2821 gctggctcac cggccccgtg tggttcaccg agcggccgga cacgaacttc cacgacttca
     2881 tgtcgagcat gatggacgcg caggtcgaag gcgcgctcgg cggcaacgcc ttcgagcact
     2941 tcgcgatgag cgtggattac gggcgcgcaa aggcgtattt ccgctgcgtg agcggctgcg
     3001 tccccgcttc cgggccctga aagctcttgt tgccgagaag cctgtcccgg acccgatccg
     3061 gagcaatccc gtaaacccgg cccctgctct acaatcgcag aagcgccgcc gcagcctgct
     3121 ccgcggcaat catcgttcgg gcaggttcca ttccgacacc acttgcggga gttgttcatg
     3181 aaatttcgat ttgcaatgta cgcaatgctc gccccgctga ttttctccga aaccgcgcag
     3241 gcgcaggtga gcctctccgc gctggccgat cacatggcgg ggccgccgac gcagattctg
     3301 gtgctcggaa ccgcgcacct ttccaacgcg cccaagggtt tcgagccgca gtcgctgcaa
     3361 ccgctgctcg cgcgtctggc cgcgtaccag ccgcagatca tcaccatcga ggcaatttcc
     3421 ggcgaaggct gcgcgctgct ggccgagtac ccgacgatct acaacccgga aaacgtcgcg
     3481 cccttttgcg ccgacaccac cgccgccaag gccgcgaccg gcctcgacgt gccggccgcg
     3541 gtcgtcgccg cggacaaggc gctgaaggac tggccggcgg atcccacgcc ggcgcagcgc
     3601 cgccacctcg cggcgctctt tctcgcatcg ggagaaccgg cttcggcact ggtgcaatgg
     3661 ttgcaactgc cgaaggccga gcggcacaag ggcgacgggc tcgacgatgc cctggtgacg
     3721 cttctgaaga agaaacgcgc cagcaacaac gaggactacc agctcgccgt gccgcttgcc
     3781 gtgaagctgg gattgcagcg cgtgtatccg atcgacgatc ataccggcga caatatccga
     3841 atcaccgatc agcagaccaa gggcttcgtc gcggcaatcc gcaaggcctg ggactcgacc
     3901 aagtcacaaa tgcagccgat cgaggaccgg gagcacgatc tgctcgaaca caaccacacg
     3961 ctggagctgt atcgctatct caacagcccc gacgtacagc aagctttcat cgcaagcgac
     4021 atgggcgccg cgcttcgcga cgactcggcg cagcactacg gtcagatgta tgtcaccggc
     4081 tgggaagccc gcaatctccg catggcggcc aacatccttg caaccttccg cgagcacccc
     4141 ggtgcccgcg tgctcaccct cgtcggcgcc acccacaagc cctggctcga caaaatcctc
     4201 ggccagatgc cgggcgtgga aatcgtggac acggaaaaga tgctgggcgc ggcggcgcag
     4261 taagcgaaaa cggcgggacg cggaagcgcc acgcaagcgg cgtcggcgcc agactcc
//
LOCUS       DASUJG010000311         2338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_88279, whole genome shotgun sequence.
ACCESSION   DASUJG010000311 DASUJG010000000
VERSION     DASUJG010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88279"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1730
                     /locus_tag="VFX38_06680"
     CDS             <1..1730
                     /locus_tag="VFX38_06680"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX5314569.1"
                     /translation="QINPLVGDVAGNTRRVLDQVATHRGECDLIVFPELVLTGYPPED
                     LLLHPDMRRQIAEALAQLRSAAGESRLLVGHPEYQNGWIYNACSLIAGGEIRAVYRKR
                     ELPNYGVFDEKRHFTPGSEPLVVDVAGLPIGVTICEDGWIEGPIADTCKAGARLMINV
                     NASPYAIGKRAEREAVIAARAKASGVATLYLNLVGGQDELVFDGHSFVVDRHGEVIER
                     MAGFREDFAVFRISTRAGSRGHVAGRVGGEKAAASGEEESEEFGAGFADDATGEPDAE
                     VYQALTLAVHDYAIKNGFKGVLVGLSGGVDSALTLAIAVDALGAGQVAAVFMPSRYTS
                     ELSGELAKEQVRRLGVKAHTVSIEEPFQAFANVLKDVFAGLEADVTEENIQARCRGVI
                     LMAISNKTGKLVLTTGNKSEYATGYATLYGDMAGGFAPLKDVSKTLIYRLARYRNHLG
                     ELAPIPERVLTREPSAELAANQKDTDSLPPYPVLDAVLAGLVEKNLGLPDLEREGFDR
                     ETVQRIARLVKRSEYKRRQAPPGVKISPRAFGRERRYPITSGFRTEGEDRGGCVERRN
                     GKNRSRSGG"
     gene            complement(1761..>2338)
                     /gene="bamD"
                     /locus_tag="VFX38_06685"
     CDS             complement(1761..>2338)
                     /gene="bamD"
                     /locus_tag="VFX38_06685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX5314570.1"
                     /translation="ETAKQADEFVQENPASPYVPYAMFMKGAAYANALQPGPLDVVFR
                     TDLGKRDPVDDQQAFTAFQQLVSRYPDSRYAKQAKQWMVFVRDRLANFDLNVAHFYAN
                     RRQWVGAVDRAAEIVTRFPQTPAAKPALKIMMQGYRALGEDRLAAAAEKYYQYNFENA
                     TPGSGAQSAPSAAPAAITTPAPNAASGNSPD"
BASE COUNT          390 a          767 c          802 g          379 t
ORIGIN      
        1 cgcagatcaa tccgctcgtc ggagacgttg ccggaaatac ccgccgcgtg ctcgaccagg
       61 tcgccaccca ccgcggcgaa tgcgatctta tcgtgttccc cgagctggtc ctcacgggct
      121 atccgcccga ggatctgctg ctccatcccg acatgcgccg ccagatcgcg gaggcgctcg
      181 cgcagcttcg ctccgcggcc ggcgagtcgc gcctgctcgt cggccatccc gagtatcaga
      241 acggctggat ctacaacgcc tgctcgctca tcgccggcgg cgaaatccgt gccgtgtatc
      301 gcaagcgcga actgccgaat tacggagtct tcgacgagaa gcgccatttc acccccggca
      361 gcgaaccgct ggtcgtggac gtggccgggc tcccgatcgg agtcacgatc tgcgaggacg
      421 gctggatcga ggggccgatc gccgacacct gcaaggcggg ggcgcgtctg atgatcaacg
      481 tcaacgcctc gccctacgcg atcggcaagc gcgccgagcg cgaagccgtg atcgcggcgc
      541 gcgcgaaggc gagcggcgtt gcgacgttgt atctgaacct cgtcggcggg caggacgaac
      601 tcgtgttcga cggccactcc ttcgtcgtcg atcgccacgg cgaagtcatc gaacgcatgg
      661 cgggattccg cgaagacttc gccgtgttcc ggatttcgac ccgcgccgga agccgcgggc
      721 acgtcgcggg gcgcgtcggc ggcgaaaagg cagccgcgag cggcgaggag gagagcgagg
      781 aattcggagc cggattcgcc gacgatgcga cgggcgaacc cgacgccgag gtctatcagg
      841 cgctcaccct cgcggtgcac gactacgcga tcaagaacgg attcaagggc gtgctcgtcg
      901 ggctctcggg cggagtggat tcggcgctca cgctcgcgat cgccgtggac gcgctcggag
      961 cgggccaggt ggcggcggtc ttcatgccgt cccgctacac gagcgaactc agcggcgaac
     1021 tcgcgaagga gcaggtgcgc cgtctcggcg tgaaggcgca cacggtctcg atcgaggagc
     1081 cgttccaggc cttcgccaac gtgctcaagg atgtcttcgc ggggctcgaa gccgacgtga
     1141 ccgaggagaa cattcaggcg cgctgccgcg gcgtgatcct gatggcgatc tcgaacaaga
     1201 ccggcaaact cgtgctcacg accggcaaca agagcgaata cgcgaccggc tatgcgacgc
     1261 tctacgggga catggcgggc ggcttcgcgc cgctcaagga tgtctccaag acgctcatct
     1321 atcgcctcgc gcgctaccgc aatcatctcg gcgaactcgc tccgattccc gaacgcgtgc
     1381 tgacgcgcga gccttcggcc gaactcgcgg cgaaccagaa ggataccgac tcgctgccgc
     1441 cgtatccggt gctggatgcg gtgctcgccg gcctcgtgga aaagaacctc ggccttcccg
     1501 atctggagcg cgaaggcttc gatcgcgaga ccgtgcaacg catcgcgcgc ctcgtcaagc
     1561 gcagtgaata caagcgccgt caggcgccgc cgggagtgaa gatttccccc cgcgccttcg
     1621 ggcgcgaacg gcgctacccg attacctccg ggttccgcac ggaaggcgag gatcgcggcg
     1681 gctgcgtcga gcggcgcaac gggaagaatc gttcgcgctc cggcggctga gcgccggagc
     1741 aggcgcgggg aatccgcgaa tcagtcgggg gagttgccgc tcgctgcgtt cggcgcgggc
     1801 gtcgtgattg ctgccggcgc tgcgctcggt gcggactgcg caccggaccc gggcgtcgcg
     1861 ttctcgaagt tgtactggta atacttttcc gccgccgccg cgagccggtc ctcgcccagc
     1921 gcccggtagc cctgcatcat gatcttgagc gcgggtttcg ccgcgggagt ctgcgggaag
     1981 cgcgtcacga tctcggcggc gcgatccacc gctcccaccc attggcggcg attggcgtag
     2041 aagtgcgcga cgttcagatc gaagttcgcg agccggtcgc gcacgaagac catccattgt
     2101 ttcgcctgct tcgcgtagcg cgagtccggg tagcggctca cgagctgctg gaaggcggtg
     2161 aaggcctgct ggtcgtcgac cggatcgcgt ttgccgagat cggtgcgaaa gaccacgtcg
     2221 agcggtccgg gctgcagggc attggcatag gccgcgccct tcatgaacat ggcgtaggga
     2281 acgtaggggc tggcgggatt ctcctgcacg aattcgtccg cctgcttggc cgtctcgt
//
LOCUS       DASUJG010000312         3991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1677870, whole genome shotgun sequence.
ACCESSION   DASUJG010000312 DASUJG010000000
VERSION     DASUJG010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1677870"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="VFX38_06690"
     CDS             complement(<1..904)
                     /locus_tag="VFX38_06690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX5314571.1"
                     /translation="MKPLDFSTPPASVCVLRLSALGDVCHALPVVNTLRAAWPATRFS
                     WIVGRTEASLIGDIPDIEFLSFDKAAGWPAYRELRRRLAGRRFDLLLHMQLSLRASLA
                     SRAVRAPLRLGFDRERAHDWQWLFTNRRIAHVAREHVMDSFFGFARACGVSERRLEWN
                     IPVPPAAAARAREFIPDGMPALLVSPCANARFRNWRNWPYERYGPVADYAAGTLGWRV
                     IVTGGPSAAEREAARAIVAASRTGIVNLQGKTSLKELLALIARAQVIITPDSGPAHMA
                     TAVGTPVIGLYATTNPERAGPYLSQ"
     gene            903..1601
                     /locus_tag="VFX38_06695"
     CDS             903..1601
                     /locus_tag="VFX38_06695"
                     /EC_number="2.7.1.166"
                     /inference="COORDINATES: protein motif:HMM:NF002475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid kinase"
                     /protein_id="HEX5314572.1"
                     /translation="MRFERTEIAGGFILADAALTPRAEWFEPQHWKRRGAGEPLGAGR
                     GIAVSAGEAGAWALRHYHRGGWPGRFVADSYLWRGAERARPVRELRLLAALRERGAPV
                     VQPVAARVLRAGLRYRGDILTQRIRDSRTLGSCARTLEPEDWARIGEAIRRLHEAGGW
                     HADLNAHNILLTPCDVFVLDLDRGRLVPSGSRVQRRNLDRLQRSLGKLGLLPPVASGW
                     QTLLSAYRSPSKPK"
     gene            complement(1567..3576)
                     /locus_tag="VFX38_06700"
     CDS             complement(1567..3576)
                     /locus_tag="VFX38_06700"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX5314573.1"
                     /translation="MKGTLHLVALALLALVMLPPPAAARTLSVQDWIRRPEYTQAVIS
                     PDGKYLAVVMTQKEGSGDYELVVMNTAALLAGKVQVSARIGLGGQELFGDVFWASDNR
                     VVMSTERYVGGFDRPYPDGRLMSISPHGGRGVTLMGRYAGLLWLPNKNTKEIVVSGYP
                     DGNKLSAYRLDVVTGAIRRIGTAPFAGGMLTDHTGKVRLEWGFNELTGWPKLLYRPAD
                     SLNWKDVSSLVNTNKDAAAALMTGGPVMFGPDNKTVYVKSWADNPAETLGLYSFDFDT
                     GAEKLLYANPAVDVDRTLSSFERDSLVGLQIEPGRPETLALNAQAPRMQLLAALQQAL
                     PGVQTNTVSWTRDGSEAVVETWSDTEAPAFYLYAGKPKPALQLLFRSTPWINPDDLSP
                     MQPVSYPSRDGLTIHGYLATPRGAAPKDLPLVVYVHGGPYGLRNSWGFDPSDFDSVAI
                     QILANHGYAVLAPNYRGSGGYGFKFLQAGFRHWGDTMQDDLADGVAWAVKQGYADPKR
                     VCILGASYGGYAALMSAERFPDLYRCAIGYSGVYDLAMMESRKTDFSRYAFGNLYTSI
                     VLGKDAAQLRAFSPADNAGSLKASVLLLHGGRDERAPVAGYDEMVAAIKAHGTPLETL
                     FYHNEGHGFYQPEHRTKAWDDILDFLGRSIGPAGLTSASTGSGTH"
     gene            complement(3655..>3991)
                     /locus_tag="VFX38_06705"
     CDS             complement(3655..>3991)
                     /locus_tag="VFX38_06705"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX5314574.1"
                     /translation="ILNLSQSGTLIQSMQLARSVMDQLNDSLPMHSSKVERAAFVVLK
                     SPDVPSILVETAFISNRSQDQELASNDFRSRIAGMIATGIEDYANHFAPPGTLIAARR
                     DAINTLKQG"
BASE COUNT          685 a         1256 c         1369 g          681 t
ORIGIN      
        1 gctggctgag atagggaccg gcgcgctcgg ggttggtcgt cgcgtaaagc ccgatcacgg
       61 gcgtgccgac ggccgtcgcc atgtgcgcgg gccccgaatc gggagtaatg atgacctgcg
      121 cgcgggcgat cagggcgagg agttccttga gggacgtctt gccctgcaga ttgacgatcc
      181 cggtgcggct cgcggcgacg atcgcgcgag ccgcctcgcg ttccgccgcg ctcggcccgc
      241 cggtgacgat cacgcgccag ccgagcgtcc ccgcggcgta atcggcgacg ggaccgtagc
      301 gctcgtacgg ccagttgcgc cagttgcgaa agcgcgcatt ggcacaggga ctgacgagca
      361 aagccggcat gccgtcggga atgaattcgc gggcgcgcgc cgccgccgcg ggcggcacgg
      421 gaatgttcca ttcgagacgc cgctcgctca cgccgcaggc gcgcgcgaaa ccgaagaagc
      481 tgtccatcac gtgttcgcgc gctacatgcg caatgcgtcg gttcgtgaac agccactgcc
      541 agtcgtgggc gcgttcgcga tcgaagccga gccgcagcgg cgcgcgaacc gcccgactcg
      601 cgaggctcgc acgcagcgac agttgcatgt ggagcagcag atcgaagcgc cgcccggcga
      661 gccgccggcg cagttcgcga tatgctggcc atcccgcggc cttgtcgaag gagaggaatt
      721 cgatgtccgg aatgtcgccg atcaggctcg cttccgttcg cccgacgatc cagctgaagc
      781 gggtcgccgg ccaggcggca cgaagggtgt tgacgacggg aagcgcatga cacacgtcgc
      841 cgagcgccga caaacgcagg acgcaaacgc tcgcgggcgg cgtggagaaa tcgagaggct
      901 tcatgcgctt tgagcgaacc gaaatagcgg gcggcttcat cctagccgat gcggccctca
      961 cgcctcgcgc cgagtggttc gagccgcaac actggaagcg gcggggcgcg ggcgagccgc
     1021 tcggtgccgg gcgcggcatt gccgtgagcg caggcgaggc cggcgcctgg gcgctgcgtc
     1081 attaccatcg cggcggttgg ccgggccgct tcgtggccga ttcctatctg tggcgcggag
     1141 cggagcgtgc gcgacccgta cgcgagctgc gcctgctcgc cgcgctgcgc gagcgcggcg
     1201 cgccggtcgt gcaaccggta gcggcgcggg tactgcgagc gggactgcgc tatcgcggcg
     1261 acatcctcac gcagcgaatc cgggattcgc gcacgctcgg gagctgtgcg cggacgctgg
     1321 agcccgaaga ttgggcgcga atcggcgagg cgatccgccg ccttcacgaa gccggcggct
     1381 ggcatgccga tctcaacgcg cacaacattc tcctgacacc gtgcgacgtt ttcgttctgg
     1441 acctcgatcg cggccgcctc gttccttcgg gctcgcgcgt gcagcgccgc aatctggatc
     1501 gcctccagcg ctcgctcggg aaactcggct tgctgccgcc ggtcgcgtcc ggctggcaga
     1561 cgctcctcag tgcgtaccgg agcccgtcga agccgaagtg agtcccgccg ggccgatgga
     1621 gcgcccgagg aaatcgagga tgtcgtccca ggccttggtg cggtgctcag gctgatagaa
     1681 gccgtgtccc tcgttgtgat agaagagcgt ttccagcggc gtgccgtgcg ccttgatcgc
     1741 cgcgaccatt tcatcgtagc cggcgaccgg cgcgcgctcg tcgcgtcctc cgtgcaggag
     1801 aagtaccgac gccttcaggc tgccggcatt gtccgcgggc gaaaaggcgc gcagttgcgc
     1861 cgcgtccttg ccgagaacga tggaagtgta gagattgccg aaggcatagc gactgaaatc
     1921 ggtcttgcgg ctttccatca tggcgaggtc gtacacgccc gagtaaccga tcgcgcaacg
     1981 atataaatcc ggaaagcgct cggcgctcat gagcgcggcg tagccgccgt aactggcgcc
     2041 caagatacac acccgtttcg ggtctgcata gccctgtttc accgcccagg cgacgccatc
     2101 ggcgagatcg tcctgcatgg tgtcacccca gtgccggaaa ccggcttgca ggaacttgaa
     2161 gccgtaacca ccggaaccgc ggtagttcgg cgcgagcacg gcatagccgt gattcgcgag
     2221 aatctggatt gccaccgagt cgaagtccga gggatcgaag ccccaggaat tgcgcagacc
     2281 ataagggccg ccgtgcacat agaccaccaa gggcaggtct ttcggtgcgg ctcctcgggg
     2341 ggtcgcgaga taaccgtgaa tggtcaggcc atcgcgactc gggtaactca ccggttgcat
     2401 cggcgaaaga tcgtccgggt tgatccaggg cgtcgagcgg aacagcagct ggagcgcggg
     2461 cttcggcttg cctgcgtaaa ggtagaaggc cggggcttcg gtgtcgctcc aggtttcgac
     2521 caccgcctcg ctgccatccc gtgtccagct cacggtgttg gtctggacgc cgggcagggc
     2581 ttgctggagg gccgcgagca attgcatccg tggtgcctgt gcgttgagcg ccaaggtctc
     2641 gggtctgccc ggctcgatct ggagccctac cagcgagtcg cgttcgaagg acgagagggt
     2701 ccggtccacg tccaccgcgg gattcgcata cagcagtttt tctgcgccgg tgtcgaagtc
     2761 gaagctgtac aggccgagcg tttcggcggg attgtcggcc cagcttttga catacacggt
     2821 tttgttgtcg ggtccgaaca tgacgggccc cccggtcatg agcgcggccg ccgcatcctt
     2881 gttcgtgttg accagcgacg acacatcttt ccaattcagg gaatcggccg ggcgatagag
     2941 cagtttcggc cacccggtca gttcgttgaa accccattcg aggcgcacct tgccggtgtg
     3001 gtcggtaagc atgccgccgg cgaagggtgc cgtcccaatc cggcggatcg caccggtaac
     3061 cacatccaga cgatacgccg agagtttgtt gccatccggg taccctgaga cgacaatctc
     3121 tttggtgttc ttattcggta accaaaggag acctgcgtaa cggcccatca gcgtgacccc
     3181 gcgtccgccg tgcggactga tggacatgag gcggccgtcg gggtaagggc gatcgaatcc
     3241 gcccacatag cgctccgtgg acatcacgac tcgattgtcg ctggcccaga agacatcgcc
     3301 gaaaagttcc tgtccgccga ggccgatgcg cgcgctcacc tgcaccttgc cggcgagaag
     3361 cgccgcggtg ttcatcacga ccaattcgta atcgccactt ccctctttct gagtcatgac
     3421 gaccgcgaga tatttgccgt cgggcgagat caccgcctgg gtgtactcgg gccggcgaat
     3481 ccagtcctgg acggagagcg tccgggccgc cgccggcggc ggcagcatga ccagcgccag
     3541 cagagccagc gcaacgagat gaagcgtgcc tttcatgatt ccctcctccc ggtgaaattc
     3601 gcggcgaggg ggagtgtagc gccgcaaggg cgcgcgggca agcgcgggcg gggctcagcc
     3661 ctgcttgagc gtgttgatgg cgtcgcggcg agccgcgatc agggtccccg gcggcgcgaa
     3721 gtgattggcg taatcctcga ttccggtcgc gatcatcccc gcgatgcggg agcggaaatc
     3781 gttgctcgcg agttcctggt cctgactgcg attcgagatg aaggcggtct cgaccagaat
     3841 cgagggaaca tccggggatt tcagcacgac gaaggccgcg cgttccacct tgctgctgtg
     3901 catcggcagc gaatcgttga gctgatccat gacggagcgg gcgagctgca tgctctggat
     3961 gagcgtgcca ctttgcgaaa ggttgaggat g
//
LOCUS       DASUJG010000313         4027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1311734, whole genome shotgun sequence.
ACCESSION   DASUJG010000313 DASUJG010000000
VERSION     DASUJG010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311734"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1148)
                     /locus_tag="VFX38_06710"
     CDS             complement(<1..1148)
                     /locus_tag="VFX38_06710"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEX5314575.1"
                     /translation="MAKGKDKAAAGKRKEKAPAPKSAPTPKRPLIVGIGASAGGLEAF
                     KAFFHHMPADSGMAFVLVQHLDPDHHSLLAELLASHTAMPVVEAEDGAQVIADHVFVI
                     PPDATLTIEQGRLQVQTPAPARSGRHPIDAFFASLAADQGENAVCVVLSGAGSDGTLG
                     LKKIKEHGGFALAQEGLDHSAAGGMPASASATGLVDFCVPVEAMPEKLIEYQRHMHKV
                     EKRKDKQGNRSDAADHLETITELVRGAVGHDFSQYKENTLIRRVQRRMQVLQVDTVPA
                     YIERLRAEPQELRCLFDDLLIGVTQFFRDPEAWTAFERTVIPAFFEDKVAADGIRIWV
                     PGCGSGEEAYTIAILLREAMERTHKSPTVQIFGTDIDERAIEIARAGR"
     gene            1308..2270
                     /locus_tag="VFX38_06715"
     CDS             1308..2270
                     /locus_tag="VFX38_06715"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEX5314576.1"
                     /translation="MAVRSLIVVGASAGGVGALRSLAASLPGDLPAAVAVVLHIGRHR
                     SELATILDRAGPLAAAEARNDEPLVEGRIYVAGPDCHLLVAGERLHLQRGPRENFARP
                     AIDPLFRTAAESFGPRAIGVILSGRLNDGTVGLYEIKRRGGLAVVQSPEDAQYPEMPA
                     SALRHVAVDHRVALAEIPPLLARLAAKLAAPTSIPRPAEEVIDMTANYVLERPTALTC
                     PECGGAMDGKDIGTLQQYRCHIGHAYTAEAMASAHLDRMEAGMEKALRLLNERIEICR
                     RMAERPEVPDEKMKAAWFGAMEQAEQRADAMGELLSAGWLAPEE"
     gene            2658..3119
                     /locus_tag="VFX38_06720"
     CDS             2658..3119
                     /locus_tag="VFX38_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5314577.1"
                     /translation="MLQVRRAGIGDLDRLVPLFEEYRHFYGRESPKPETRAFLRERIE
                     GDESVVFLAMLDALPAGFTQLYPLFSSVGMSRIWLLNDLYVVAAARRRGVASALLDAA
                     ETHARKTGATALLLQTGRDNYGAQTLYERKGWIREDDYCWYDLAISPAPPD"
     gene            complement(3255..3830)
                     /locus_tag="VFX38_06725"
     CDS             complement(3255..3830)
                     /locus_tag="VFX38_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314578.1"
                     /translation="MTISESFVQQVVLLLIAAAVTGFGLPYVLRLVDNRKLRQQKQFE
                     AELARQNKLIEAQSALLDEITRLVWGWRYLAKQVVYYGTGDDTDRYAAASAAYEERVW
                     KMLDGFRTEISKSRRLVSETAFRELNALYEYIVGDIDRKVGEFAGKPEINRKACAELS
                     TRFTDEVSARLDDALFKLASELRLTSHSVGV"
BASE COUNT          696 a         1331 c         1250 g          750 t
ORIGIN      
        1 tagcgcccgg cgcgggcgat ttcgatcgcg cgctcgtcga tgtcggttcc gaagatctgc
       61 accgtcggcg acttgtgcgt gcgctccatc gcctcgcgga gcaggatcgc gatcgtgtag
      121 gcttcctcgc cgctgccgca gcccgggacc cagatgcgaa tcccgtcggc cgcgaccttg
      181 tcctcgaaga acgccggaat gacggtccgt tcgaacgccg tccaggcctc gggatcgcgg
      241 aagaactggg taaccccgat cagcagatcg tcgaagaggc atcgcagctc ctgcggctcg
      301 gcgcgcaggc gctcgatgta ggcgggaacc gtgtccacct gcaagacctg catgcggcgc
      361 tgcacccgcc ggatcagcgt gttctccttg tactgactga agtcgtgccc gacggcgccg
      421 cgtaccagct ccgtgatcgt ttcgagatga tcggcggcgt cgctgcggtt gccctgcttg
      481 tccttgcgct tctcgacctt gtgcatgtgg cgctgatact cgatcagttt ctccggcatc
      541 gcctccaccg gcacgcagaa atccacgagg ccggtggcac tcgcgctcgc gggcatgccg
      601 cctgcggccg aatggtcgag cccttcctgt gcgagggcga agccgccgtg ttccttgatt
      661 tttttcagcc ccagcgtgcc gtcgctcccg gctccggaaa gtacgacgca tacggcgttc
      721 tcgccctgat cggcggcgag cgaggcgaag aaggcgtcga tcggatggcg gccgcttcgc
      781 gccggcgccg gcgtctgcac ctgcaggcgt ccctgttcga tggtgagcgt cgcatccggg
      841 ggaatgacga agacgtgatc ggcgatcacc tgcgcgccgt cctcggcttc gacgaccggc
      901 atcgcggtgt gcgaggcgag aagttcggcg agcaaactgt gatgatcggg atcgagatgc
      961 tgcaccagca cgaaggccat gccgctgtcc gccggcatgt gatggaaaaa ggccttgaat
     1021 gcttcgagcc cgccggcgga ggcgccgatg ccgacgatca gcggtcgttt cggtgtcggc
     1081 gccgatttcg gggccggcgc cttttccttg cgctttccgg cggccgcctt gtccttgccc
     1141 ttcgccatcg cgatccctcc gggtgcaggt cgggaaacca tggctttccg ctgcccgcag
     1201 tgtagcgcgc ggcttcggca aattccagtg gtctgcgccg gcccctcctg ttttttcgcc
     1261 gccggggcgg actatagtga aaagacgcca cagcaacagg tggaaagatg gcggttcgca
     1321 gcctcatcgt cgtcggtgcc tctgccggcg gagtcggcgc cctgcggagc ctcgcggcga
     1381 gcctgcccgg agacctcccg gccgcggtgg ccgtggtgtt gcacatcggg cggcatcgca
     1441 gcgaactcgc gacgattctc gatcgcgcgg ggccgctcgc cgccgccgag gcccgcaacg
     1501 acgagccgct cgtcgaagga cgcatctatg tggccggccc ggactgccat ctgctggtcg
     1561 cgggcgagcg cctgcacctt cagcgcgggc cgcgggagaa cttcgcccgt ccggcgatcg
     1621 accccctgtt tcgcaccgcc gccgaatcct tcgggccgcg cgccatcggc gtgattctga
     1681 gcggccgcct gaacgacggg acggtcgggc tctacgagat caagcgtcgc ggcggcctcg
     1741 ccgtggtgca atcgccggag gatgcgcaat atccggaaat gccagcgagc gcgctgcggc
     1801 acgtcgcggt ggatcatcgc gtcgcgctcg ccgagatccc gcccctgctc gcacgactcg
     1861 ccgcgaaact cgcggcccca acttcgattc cacgacccgc ggaggaggtg atcgacatga
     1921 ccgcaaacta tgttctcgag cgcccgacgg cgctcacctg ccccgagtgc ggcggcgcga
     1981 tggacggcaa ggatatcggc acgctccagc aataccgctg ccacatcggc cacgcctaca
     2041 ccgccgaagc catggcgagc gcgcacctcg acaggatgga agccggcatg gaaaaggcgc
     2101 tgcgccttct caacgaacgg atcgaaatct gccgccgcat ggccgaacgt ccggaagtgc
     2161 ccgacgagaa aatgaaggcg gcctggttcg gcgcgatgga gcaggcggaa cagcgcgcgg
     2221 atgcgatggg cgaactgctg agcgccggct ggctcgcgcc cgaggaataa ttcagggccg
     2281 cagcctcgcc catccgtcac ccctagcgaa gatcgggagc cggggtttgg ctcccatatc
     2341 ctcttcccgt ctgtcgcccc ggcgaaggcc ggggccccgt ccacaaagta tcccgcgcaa
     2401 ggcgcgggat tcgttatttg actggattcc ggcctgcgcc ggaatgacgg ggtgtgggat
     2461 tggcggcttg actggattcc ggcctgcgcc ggaatgacgg gttccgtggt tctgtaggtt
     2521 gggctgaacg cagttttgta ggttgggctg aacgcagtga agcccaacat gcgcgcccat
     2581 gcaatgcatc gttttccgaa gcgctggatc cgatcctgcg ccaagtacaa tgtccttcgt
     2641 ttgcgcggag tattcgcatg ctgcaagttc gtcgtgccgg catcggggat ctcgaccggc
     2701 tcgttccgct gttcgaggaa taccgacact tctacgggcg cgaatcgccg aagccggaaa
     2761 cccgcgcctt tctccgcgaa cggattgaag gcgacgaatc ggtcgtcttt ctcgccatgc
     2821 tcgacgctct ccctgccgga ttcacgcaac tgtatcccct cttctcctcc gtcggcatga
     2881 gtcgaatctg gctgttgaac gatctctacg tcgttgcagc cgcccgtcgt cgcggtgtcg
     2941 caagcgcgct cctcgacgcc gccgaaaccc atgcgcgaaa aaccggcgcg accgcgcttc
     3001 tcctccagac cggccgcgac aactacggcg cgcaaaccct ctacgaacgc aagggctgga
     3061 tccgtgaaga cgactattgc tggtacgacc tcgcaatttc acctgccccc cccgactgaa
     3121 tgcaagtgga caacgccatc ccgaggaacc tctgaagaat tcgtcgtttc cgcgaacgcg
     3181 ggaaccccgt aaaagcaagg atctggattc ccgccttcgc gggaatggcg gaagtggaag
     3241 aaatcagagt ttccctaaac tcccacggaa tgggaagtca gtcggagttc ggatgccaac
     3301 ttgaacagtg cgtcgtccag tcgggccgac acctcgtcgg tgaagcgagt cgaaagctcg
     3361 gcacacgctt tgcgattgat ctccggcttc cctgcgaact cgcctacttt gcggtcaatg
     3421 tccccgacaa tatactcgta caaggcgttc agctccctga acgcggtttc ggacaccagc
     3481 ctgcgcgact tgctgatttc cgttctgaag ccatccagca tcttccatac ccgctcttca
     3541 tacgcggcgc ttgcggcagc gtacctgtcc gtgtcgtcgc cggttccgta atacacgacc
     3601 tgcttggcaa ggtacctcca gccccagacg agccgcgtga tctcgtccag caatgcagat
     3661 tgcgcttcaa tcaacttgtt ctggcgcgca agctccgcct cgaattgttt ctgctgacgc
     3721 agtttgcgat tgtctacaag cctcaacaca tagggcagtc cgaatccggt aacggccgcc
     3781 gcgatgagga ggagcacgac ctgctgaaca aacgattcgc tgatggtcat gtgacagttc
     3841 ccgccttttc ccacccaatc atgcgaaacg agacttcccg gcgcgtccgg gaggctgcac
     3901 gagccagttc attaccgcgc gatcatttcc aaatggcaac gccgtgaccc gcaatcggtg
     3961 tttcggaatt catggctgcc cgcctccgcg accggtcacg gacgaaccag gccgaggatg
     4021 gacagta
//
LOCUS       DASUJG010000314         4517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1799846, whole genome shotgun sequence.
ACCESSION   DASUJG010000314 DASUJG010000000
VERSION     DASUJG010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1799846"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VFX38_06730"
     CDS             <1..387
                     /locus_tag="VFX38_06730"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX5314579.1"
                     /translation="AANFLMAIFWAFVVRIGYGLAASIPSLALPLVLAGVAGMFINSI
                     LMVFNLVPLPPLDGGRVAASLLPPRWSSAYERVEPFGLIIVVLLLFTGVLGFVLFPIL
                     RGCFAFFSLFAGTAPGNLWYLLGGLV"
     gene            387..1253
                     /locus_tag="VFX38_06735"
     CDS             387..1253
                     /locus_tag="VFX38_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEX5314580.1"
                     /translation="MGATLESHSPPPGSQGELPFAVVQGEPLTSLPRDLYIPPDALEV
                     ILESFEGPLDLLLYLIRRQNLDILDIPIAEITRQYMNYIDVLHELRLDLAGEYLVMAA
                     MLAEIKSRMLLPRPEADGADTEEDPRAELVRRLQEYERYKQAAEDLDSLEREGRDRFA
                     VSLHLAGRRVVRVLPEIAFDSLLESFGHVIKRAALYAGHRIGREPISIRQRMSDLLAQ
                     IGDGAHRMFAALIERGEGREGVVVSFLALLELARTGFASLTQAELFGPLYVRAQGLSG
                     LANEPAGATQEE"
     gene            1253..1945
                     /gene="scpB"
                     /locus_tag="VFX38_06740"
     CDS             1253..1945
                     /gene="scpB"
                     /locus_tag="VFX38_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008294592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX5314581.1"
                     /translation="MDVNAPAPDDILLAALFAADHPLTREELGALVEIGDVPAPPPPG
                     EVDAALARIDERLRGLPLELKKLASGYRIEVPTSYATWVSRLWVERPARYSRALLETL
                     ALIAYRQPITRPEIEAVRGVAVNTQIVRALTDRGWIRTVGHRDAPGRPALYGTTRSFL
                     DYFGLASLEELPTLAEIRELADFEPELPLENLATAEQEEAAPEQLGEDRDGGAPAEIL
                     RGPWRADGEDGA"
     gene            1935..2696
                     /locus_tag="VFX38_06745"
     CDS             1935..2696
                     /locus_tag="VFX38_06745"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004139959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX5314582.1"
                     /translation="MEREAADRPERLLAGLGIASRREIARWIAAGRLAAGGRVLRGGE
                     RIRASDRLTLDGKPLILPAEQAARRVLLYHKPVGEIVSRHDPDGRPSVFAALPPLRGG
                     RWITVGRLDITTAGLLLFSSDGALAARLMHPRYGIERRYLVRVHGDAPPQRIEELTRG
                     VRLEDGMARLSACAPLAKRPNDAANRWYRVSLEEGRNRELRRIFAAVGLEVSRLKRIG
                     YGPISLPRGLPAGSWVELAAGEIAALDAAVGGHEA"
     gene            2844..3419
                     /gene="rpoE"
                     /locus_tag="VFX38_06750"
     CDS             2844..3419
                     /gene="rpoE"
                     /locus_tag="VFX38_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007190970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HEX5314583.1"
                     /translation="MSGPEADRLLVARAKTGDQAAFNALVIKYQQRIVKLVSRYIRDP
                     EEALDVSQEVFIRAYRAIGSFRGDSAFYTWLYRIAVNAAKNAIGAAMRNPVEGGLDVD
                     DPELSEVSLKMTDVSSPERLLYTEELKQTINAAMDELPPELRRAIALRELEGLSYEAI
                     AQAMDCPVGTVRSRIFRAREAIAHRIQPLLE"
     gene            3424..4011
                     /locus_tag="VFX38_06755"
     CDS             3424..4011
                     /locus_tag="VFX38_06755"
                     /inference="COORDINATES: protein motif:HMM:NF015810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HEX5314584.1"
                     /translation="MSNLLHEQISAFMDGELPAEECELLLRRLASNRELVRRWHAYHL
                     IGDTLRGQVASHAARDIAPRVGEALAPHAARARVRRPHRFTTLAAGITLVGLAGVVGA
                     LVSQQFGGGRVFTSGPTAVVSHGVGTASAQQINWQQAPTPVRAELNRYLMRHDLYGPV
                     SSALAEGATANAVVLTAASPLPVAGTGQDLNPPRQ"
     gene            4008..>4517
                     /locus_tag="VFX38_06760"
     CDS             4008..>4517
                     /locus_tag="VFX38_06760"
                     /inference="COORDINATES: protein motif:HMM:NF015825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB
                     domain-containing protein"
                     /protein_id="HEX5314585.1"
                     /translation="MTAGARAALVLAAILCPVIAEAAAAETAQSWYARMQKALATQSY
                     EGVIVYMGNGRPGSYRLVACSDGYARLTALLGPAREVIRGPNIAIRRRADGSSMVVHG
                     LAGDGSMLPFPPASRADAAALNHYRLNLAGWGRVAGQSARVLAFEPTDDWRYGYRVWI
                     SDDSGLPLRS"
BASE COUNT          671 a         1539 c         1506 g          801 t
ORIGIN      
        1 gcggccaact ttctcatggc gatcttctgg gccttcgtcg tgcggatcgg ctacggtctc
       61 gccgcgagca tcccttcgct cgcgctgccg ctcgttctcg ccggggtcgc cggcatgttc
      121 atcaattcga ttctgatggt gttcaacctc gtcccgctcc cgccgctgga cggcgggcgc
      181 gtagcggcca gcctgctgcc gccgcgctgg tccagtgcct acgagcgcgt cgagcctttc
      241 gggttgatca tcgtggtact gttgctcttc accggcgtgc tcgggttcgt gctttttccg
      301 attctccggg gctgcttcgc gttcttttcg ttgttcgcgg ggacggcgcc gggcaatctc
      361 tggtatctcc tcggtggcct tgtctgatgg gtgcaacgct ggaatcgcat tcgccgccgc
      421 ccggaagcca gggcgagctg cccttcgcgg tggtgcaggg cgaaccgctg acgtccctgc
      481 cgcgtgatct ctatattccg cccgatgcgc tcgaggtgat tctcgaatcc ttcgagggcc
      541 cgctcgatct tctgctctat ctcatccgcc ggcagaatct cgacattctc gatattccga
      601 tcgccgaaat cacccgtcag tacatgaact acatcgacgt tctgcacgaa ctgcggctcg
      661 atctcgcggg agagtatctc gtcatggccg cgatgctcgc cgaaatcaag tcgcggatgc
      721 tgctgccgcg acccgaggcc gacggcgccg acaccgaaga ggatccgcgc gccgaactgg
      781 tgcggcgcct gcaggagtac gagcgctaca agcaggcggc cgaagatctc gattccctcg
      841 agcgcgaagg tcgcgatcgc ttcgcggttt cgctgcatct cgccggtcgg cgcgtggttc
      901 gcgtgcttcc cgagattgcc ttcgattcgc tgctcgagag ttttggccac gtgatcaaac
      961 gcgcggcgct ttacgcggga catcgaatcg gccgcgagcc catttccatc cgccagcgca
     1021 tgagcgatct tctcgcgcag atcggcgatg gcgcccaccg gatgttcgcc gcgctcatcg
     1081 agcgcggcga agggcgggag ggcgtcgtcg tcagtttcct cgcccttctc gaactcgcgc
     1141 gcaccggctt tgcgagcctc acgcaggcgg aactcttcgg cccgctttac gtccgggcgc
     1201 agggcctgag cggactcgcg aacgaacccg caggcgcgac gcaggaggag tgatggacgt
     1261 gaacgccccc gcaccggatg acattctgct cgccgcgctc tttgccgcgg atcatccgct
     1321 cacgcgcgag gaactcggag cgctcgtgga aatcggcgat gtgcccgccc cgccgccgcc
     1381 gggcgaagtc gatgcggcgc tcgcgcggat cgacgagcgt ctgcgggggc tcccgctcga
     1441 attgaagaag cttgcgagcg gctatcggat cgaggtcccc acgagctatg ccacctgggt
     1501 gagccgtctc tgggtggagc gtccggcccg ctattcgcgc gcgctgctcg agaccctcgc
     1561 gctcattgcc tatcgccagc cgatcacccg gccggaaatc gaggcggtgc gcggggtcgc
     1621 cgtgaacacc cagatcgtcc gcgcgctcac ggatcgcggc tggattcgca cggtcggaca
     1681 tcgcgacgct cccggccgtc cggcgctcta cggcaccacc cgctcgtttc tcgattattt
     1741 cggcctcgca agtctcgagg aattgccgac gctcgcggag attcgggaat tggcggattt
     1801 cgagcccgag ctgccgctgg agaatcttgc gaccgcggag caggaggagg ccgcgccgga
     1861 gcaactcggc gaagaccgcg acggcggcgc ccccgccgag attcttcgtg gaccctggag
     1921 ggcggacggc gaggatggag cgtgaagccg cggatcgtcc cgagcgtctg ctcgcaggtc
     1981 tcgggatcgc ctcgcgtcgc gagatcgcac gctggatcgc ggcgggccgc cttgccgccg
     2041 gcggccgggt gcttcgcggc ggggaacgca ttcgcgcttc cgatcgcctg acgctcgacg
     2101 gaaaaccgct gattctgccg gccgagcagg ccgcgcgccg ggtacttctc tatcacaagc
     2161 cggtcgggga aatcgtgagc cgccacgatc ccgacgggcg accgagcgtc ttcgccgcct
     2221 tgccgccgct tcggggcggg cgctggatca ccgtcgggcg actcgacatc accaccgcgg
     2281 gcctgcttct gttcagctcc gacggggcgc tcgcggcacg gctcatgcac ccgcgttacg
     2341 ggatcgaacg tcgctatctc gtgcgcgtcc acggcgatgc gccgccgcaa cggatcgaag
     2401 agctgacccg aggcgtgagg ctcgaggacg gcatggcgcg gctgagcgcc tgcgcgccgc
     2461 tggcaaagcg gccgaacgac gccgccaacc gctggtatcg ggtcagccta gaggagggac
     2521 gcaatcgcga attgcgccga atttttgccg ccgtcggact ggaggtcagc cgcctgaaac
     2581 ggatcggcta cggtccgatc tcgctgccgc ggggtctccc cgccggaagc tgggtcgaac
     2641 tcgccgcggg cgagatcgcc gcgctcgacg ccgccgtcgg cggccacgaa gcctgaggcg
     2701 ggcgcggccg gcgccccgag cctgccagcg tagaatcgtt attggaggcg gtcgtccgcc
     2761 ggagtgcgaa ccaatcttgt gcaatcctgt ccaacgcgct gatgttgacg ccgacagcag
     2821 ccacggctcc gggggccggc atcgtgagcg gtcctgaagc ggaccgcctc ttggtcgcgc
     2881 gcgccaagac cggtgatcag gcggcgttca acgcgctcgt catcaagtat cagcagcgca
     2941 tcgtcaagct ggtctcgcgc tacatccgcg accccgaaga ggcgctcgat gtctcccagg
     3001 aggtcttcat ccgcgcctac cgcgcgatcg ggagcttccg cggcgacagc gccttttaca
     3061 cctggctcta ccggatcgcg gtcaacgccg ccaagaacgc gatcggggcc gcgatgcgca
     3121 atccggtcga aggggggctc gacgtggacg atccggagct gagcgaagtg agcctcaaga
     3181 tgaccgatgt ctcgagtccc gagcgcctgc tctataccga agagttgaag cagacgatca
     3241 acgcggccat ggacgagctt ccgccggaac ttcgccgcgc aatcgcactc agagaactcg
     3301 aaggtttaag ctacgaggcg atcgcccagg cgatggactg tccggttggc acggtgcgct
     3361 cccgcatctt ccgcgcccgc gaggcgatcg cccaccggat ccagcctctt ttggagtgac
     3421 agagtgagca atctcctgca cgaacagatt tccgcattca tggacggcga gttgccggcc
     3481 gaagagtgtg agctgctcct gcggcgcctc gccagcaatc gcgaactcgt acggcgatgg
     3541 cacgcctacc atctgatcgg cgacacgctg cgcggccagg tggcctcgca cgcggcccgc
     3601 gacattgctc cccgggtcgg cgaggcgctt gctcctcatg cggcgcgggc ccgtgttcgc
     3661 cgtccgcatc ggttcacgac gctcgcggcg ggaatcacgc tggtcggtct cgccggcgtc
     3721 gtgggagcgc tcgtgtcgca gcagttcgga ggcggtcgcg tgtttacttc cggcccgacg
     3781 gccgtcgtgt cccacggggt cggcacggct tccgcccagc agatcaactg gcagcaggct
     3841 cccacgccgg tgcgcgccga actcaatcgc tatctcatga ggcatgatct ttacgggccg
     3901 gtgtcctcgg cgcttgccga gggcgcaacc gccaatgcgg tcgtcctgac ggccgcctct
     3961 cccctgcctg ttgccggtac cggacaggac ctgaacccgc cccggcaatg actgccggcg
     4021 cgcgcgccgc gctggtactc gccgcgatcc tctgtcccgt cattgccgaa gccgccgcgg
     4081 cggagacggc gcagtcctgg tacgcgcgca tgcagaaggc gctcgcgacc caaagctacg
     4141 agggcgtcat cgtctacatg ggcaacggtc gtccgggctc gtaccggctc gttgcctgca
     4201 gcgacgggta tgcccggctc acggcgctcc ttgggccggc gcgcgaggtc atccgtggac
     4261 cgaacatcgc aatccggcgg cgtgcggacg gcagcagcat ggtcgttcac ggactcgccg
     4321 gcgacggctc gatgttgccg tttcccccgg cgagccgcgc cgatgccgcg gcgctgaatc
     4381 attaccgctt gaatcttgcc ggctgggggc gggtcgcggg gcagtccgcg cgggtactcg
     4441 cgttcgaacc gaccgatgac tggcgttacg ggtatcgcgt gtggatttcg gacgattccg
     4501 gcttgccgct gcgctcc
//
LOCUS       DASUJG010000315         2252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_456025, whole genome shotgun sequence.
ACCESSION   DASUJG010000315 DASUJG010000000
VERSION     DASUJG010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456025"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..804)
                     /locus_tag="VFX38_06765"
     CDS             complement(55..804)
                     /locus_tag="VFX38_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX5314586.1"
                     /translation="MSGQFGLILIGDELLSGKRRDAHLANAAHLLGERGLAVDWVRIE
                     HDQPDALVRVFGEAIASGDIVFSYGGIGATPDDVTRPAAAAAAGLPLVRHPAIVRLLE
                     ERFGKAAYPNRIRMAEVPEGARLIPNPPGLIPGFGVAEVNFLPGFPDMSARMLAWLLD
                     ERYRSLHHARSVEQLIEVLDTPESELVPLMEQLLLAHPGVNLSSLPAGGRPWRVEIGL
                     KGLPAAVDAATAFLAAGLNAGAIPWNLRERL"
     gene            complement(801..1874)
                     /locus_tag="VFX38_06770"
     CDS             complement(801..1874)
                     /locus_tag="VFX38_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017485207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEX5314587.1"
                     /translation="MNGKEGGAIRGRGSAARPEGRFESLAHEREDDGWFPPEEEGRPR
                     TTLGVDRARRVLSDNDSPDVGFSRSINPYKGCEHGCAYCFARPTHSYLGLSPGLDFET
                     KIFYKPDAPEQLARELAAPNYRSTPIALGINTDAYQPIERETRLTRRILEVLAAHAHP
                     LTIVTKSALVERDLDLLAPMAARGLASVAFSITTLDRELARRMEPRATAPARRIEAVR
                     RVAAAGVPVSVLVAPVIPALNDMELERVLECAREAGAEHAGYVVLRLPHELAELFPAW
                     LEAHYPERKGKVLSILTELHGGKLYDSRFGTRMRGQGEWGSLLAQRFALARRRLGYSE
                     KWASLRSDLFRVPGRAEQLGLAL"
     gene            1935..>2252
                     /locus_tag="VFX38_06775"
     CDS             1935..>2252
                     /locus_tag="VFX38_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314588.1"
                     /translation="MQCPSSDSPPQPSLSARAFLLAFDRAVRSLFRIEESGLPAVLPR
                     PCLIVANHRRDADIPILGVFLGRRRGLRITGVLPHFVAREDLFAPDFLWRYWRSPWLV
                     LPRL"
BASE COUNT          357 a          739 c          776 g          380 t
ORIGIN      
        1 gcccggagca gccgccgcgc tcatcttttt gacgtcgaat ttcaggatcg attgctacag
       61 gcgctcgcgc aggttccagg ggatcgcgcc cgcattgagg ccggcggcga gaaaggccgt
      121 cgcggcatcc accgccgcgg gcaagccctt gaggccgatc tcgacgcgcc acgggcggcc
      181 tcccgcgggg aggctggaaa gattcacccc ggggtgggcg agcaggagct gctccatcag
      241 gggcacgagt tcgctctccg gcgtatcgag cacttcgatc aactgctcga cgctgcgtgc
      301 atgatggagc gagcgatagc gctcgtcgag aagccaggcg agcatgcgcg ccgacatgtc
      361 cggaaagcca ggaagaaaat tgacttcggc cacgccgaag cccgggatga ggccgggcgg
      421 attgggaatg aggcgcgcgc cttcgggcac ttccgccatg cggatgcggt tgggataggc
      481 ggctttgccg aaacgttctt cgagcagtcg cacgatcgcc ggatgacgaa ccagcggcag
      541 tccggcggcg gcggccgccg ccggccgcgt cacgtcgtcc ggggtcgcgc cgatgccgcc
      601 gtagctgaag acgatatcgc cgctggcgat ggcttcgcca aatacccgaa cgagcgcatc
      661 cggctggtcg tgttcgatgc gcacccagtc caccgcgagg ccgcgctcgc cgagaagatg
      721 cgcggcgttc gcgagatggg cgtcgcgacg cttgccagaa aggagttcgt cgccgatcag
      781 aatcaggcca aactgcccgc tcacagcgca agacccaatt gctcggcgcg gccgggcaca
      841 cggaaaagat cgctgcgaag cgaggcccat ttctccgaat aaccgaggcg gcggcgggcg
      901 agggcgaagc gctgcgcgag aagcgagccc cactcgccct ggccgcgcat ccgggttccg
      961 aagcgcgaat cgtaaagctt gccgccgtgc agttcggtca ggatcgaaag gacttttccc
     1021 ttgcgttcgg gataatgcgc ttcgagccag gcggggaaca gttcggccag ttcatgcggc
     1081 aggcgcagca cgacgtagcc ggcatgctcg gcgccggctt cgcgggcgca ctcgagcacg
     1141 cgttcgagtt ccatgtcatt gagcgccgga atcaccggcg cgaccagcac gctgacaggc
     1201 acgccggccg cggcgacccg gcgtactgct tcgatgcgcc gtgcgggtgc cgtcgctcgc
     1261 ggttccatcc gccgcgcgag ttcgcgatcg agcgtcgtga tcgagaaggc gacgctcgca
     1321 agcccgcgcg ccgccatcgg agcgaggaga tcgagatcgc gttcgaccag cgccgacttg
     1381 gtcacgatcg tgagcgggtg cgcgtgcgcg gcgagcactt cgaggatgcg gcgcgtgaga
     1441 cgtgtttcgc gctcgatcgg ctggtaggcg tcggtgttga tgccgagggc gatcggcgtc
     1501 gaccggtaat tcggcgccgc gagttcgcgc gcgagttgtt ccggcgcatc gggtttgtag
     1561 aagatcttgg tctcgaagtc gagtcccggc gagagcccga gataactgtg tgtcggccgg
     1621 gcaaaacaat aagcacaacc atgttcgcat cccttgtagg ggttgatcga gcgcgagaag
     1681 ccgacgtcgg gcgagtcgtt gtcgctcaag acgcggcgcg cgcgatccac gccgagcgtc
     1741 gtgcgcggcc tgccttcttc ctccggcggg aaccagccgt cgtcctcgcg ctcgtgcgcg
     1801 agtgactcga agcgcccctc gggacgtgcc gcagagccgc ggccgcggat ggcgccccct
     1861 tctttcccgt tcatgcgtac atggtacggg agcgcgcccg ttaagctatg gcgcgaagct
     1921 gtcccgatcc gaccatgcag tgcccgtcgt ccgattcgcc gccgcagccg tccctgtcgg
     1981 cgcgcgcctt tctgctcgcc ttcgatcggg cggtgcgctc gctgttccgc atcgaggaaa
     2041 gcggtcttcc cgcagtgctg ccgcggccct gtctgatcgt cgccaatcat cgacgcgacg
     2101 ccgatatccc catactcggc gtgtttctgg gacgccgccg cgggctgcgc atcaccggcg
     2161 tcctgccgca tttcgtcgcc cgggaggatc tcttcgcgcc ggattttctg tggcgatact
     2221 ggcgctctcc ctggctcgtc ctgccccggc tc
//
LOCUS       DASUJG010000316         2553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_89207, whole genome shotgun sequence.
ACCESSION   DASUJG010000316 DASUJG010000000
VERSION     DASUJG010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89207"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..662)
                     /locus_tag="VFX38_06780"
     CDS             complement(33..662)
                     /locus_tag="VFX38_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX5314589.1"
                     /translation="MEHVFNFLLHMDRYLAVFTQEHGLWVYALLFIVVFAETGLVVTP
                     FLPGDTLLFIAGALAGSGLISAPATAAIFAIAAILGNLSNYAIGRAIGPRVFKSRSRW
                     LSRRHLEEAHAFFERHGGKTIVLARFLPIIRTFAPFIAGVSAMSWLPFFAYTLAGAIA
                     WVAIVFTGGMFFGNLPFVRDHLTLVILGLVIVSLIPAVIFDLRRRRRRV"
     gene            758..1435
                     /locus_tag="VFX38_06785"
     CDS             758..1435
                     /locus_tag="VFX38_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5314590.1"
                     /translation="MNTPDPGCVEIEPQASAQASVIWLHGLGADGHDFGPVVPELALP
                     PDLAVRFVFPHAPVRAVTLNGGMPMRAWFDIARLDFEGRWDEAGVAQTMERVEALIAR
                     EEARGVARSRVVLAGFSQGGAVACEYLIRGPGGLAGVAVLSSFHPAGVLGTPRAGAGA
                     PPVFAAHGLYDPVVPFRLGERTQAALTAAGYPVQWRSYPMAHQVCAAELLALGAWLRL
                     RLAASPA"
     gene            complement(1452..2030)
                     /locus_tag="VFX38_06790"
     CDS             complement(1452..2030)
                     /locus_tag="VFX38_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5314591.1"
                     /translation="MAKLDTHLLPAGVRAAAPDLIYPRATPDDPATNVMTDLRRHRAV
                     SIQEFETLTAAERRMIGAGVRLLLVVNPNGEITGVVTYRDLTGTRALAAATRERIAHA
                     DLAVGLVMTPAAQTEALDYSALKHARVRDVLELLGKEGRQHTLVTETTEGRTVLRGIF
                     STTQIGRQLGVPIEPGERAHSFAEIEQLIAHV"
     gene            complement(2177..>2553)
                     /locus_tag="VFX38_06795"
     CDS             complement(2177..>2553)
                     /locus_tag="VFX38_06795"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX5314592.1"
                     /translation="SLVWFFLGLALMLAGCALRQWAMSALRRCAIEATAAKPCEWAAA
                     GPYRRLRHPACAGALLLWAGLGLALTNGASLLTLALTAVLAYGLRMRAEEAARSAAFG
                     ESYRSYCLHTPRLVPRILRKTR"
BASE COUNT          442 a          795 c          912 g          404 t
ORIGIN      
        1 aaagggcgat gcgcgctcgc gccttcgccg cttcacacac gtctgcgtcg gcggcgcaga
       61 tcgaagatca cggcgggaat gagcgagacg atcacgaggc cgaggattac gagcgtgaga
      121 tggtcgcgca cgaagggaag attgccgaag aacatcccgc ccgtgaacac gatcgcaacc
      181 caggcgatgg cgcctgcgag cgtgtaggca aaaaacggaa gccaggacat cgcgctcact
      241 ccggcgatga agggggcgaa ggtgcggatg atcggcagga agcgggcgag cacgatggtc
      301 ttgccgccgt ggcgttcgaa aaatgcgtgc gcctcttcga ggtggcgacg gctgagccag
      361 cgcgatcggc tcttgaagac gcgcggcccg atggcccgtc cgatcgcata gttcgagaga
      421 ttgccgagga tggcggcgat cgcgaagatg gcggccgtcg cgggcgcgga gatgagaccc
      481 gaaccggcga gcgcaccggc aatgaacagc agcgtgtcgc ccggaagaaa gggggtgacg
      541 acgaggccgg tctccgcaaa caccacgatg aaaaggagcg cgtacaccca cagcccgtgt
      601 tcctgcgtga agaccgcgag gtagcggtcc atgtgcagca ggaaattgaa gacgtgttcc
      661 accgggagtt ccgcgatgcg gcgacgacag tctatcccgg atgtcgctcg cggcaaagtt
      721 cgaagggcga tcgatcccgg aggctacaat cacaccgatg aatacccccg atcccggttg
      781 tgtcgaaatc gagccgcagg cgagcgcgca ggcgagcgtc atctggttgc acggactcgg
      841 tgcggacgga cacgatttcg ggccggtcgt gccggaactc gctcttcctc ccgatctggc
      901 ggtgcgcttc gtgtttccgc atgcgccggt gcgcgccgtg acgctcaacg gcggcatgcc
      961 gatgcgcgcc tggttcgaca tcgcgcgcct ggatttcgag ggccggtggg acgaagccgg
     1021 agtcgcgcag acgatggagc gagtggaagc cctgatcgcg cgcgaagagg cgcggggggt
     1081 cgcccgatca cgagtagtgc ttgccggttt ttcgcagggc ggcgcggtcg cctgcgaata
     1141 tctgatccgc gggccgggcg gtcttgccgg cgtcgcggtg ctgtcgagtt ttcatccggc
     1201 gggggttctc ggaaccccgc gcgccggagc aggcgcgccg ccggtctttg cggcgcacgg
     1261 cctttacgat ccggtcgttc cgttccggct gggggaacgc acgcaggcgg cactgactgc
     1321 ggccggctat ccggtgcaat ggcgcagcta cccgatggcg catcaggtct gtgccgccga
     1381 actgctcgcg ctcggcgcct ggttgcgcct gcgcctcgcg gcatcgcccg cctgatcgga
     1441 cccgccgcgt ctcagacgtg ggcgatcaat tgctcaattt cggcgaagct gtgcgcccgt
     1501 tcgccgggtt cgatcgggac gccgagctga cgtccgatct gggtggtcga gaaaattcca
     1561 cgcagcacgg ttcggccttc ggtggtctcg gtgaccaggg tgtgctgccg cccctccttg
     1621 ccgagcaatt cgagtacgtc gcggacccga gcgtgtttca gcgcggaata atccagtgct
     1681 tccgtctgcg cggccggcgt catgacgagg ccgacggcga gatcggcatg ggcgatgcgc
     1741 tcgcgggtcg ccgcggccag cgcccgtgtg ccggtgagat cgcgataggt cacgaccccg
     1801 gtgatttctc cgttgggatt gacgacgagg aggaggcgca cccctgcgcc gatcatcctc
     1861 cgttcggctg cggtcaaggt ctcgaactcc tgaatgctta ccgcgcggtg gcggcgcaga
     1921 tccgtcatta cgttcgttgc cggatcgtcg ggcgtcgccc gcggatagat gagatccggc
     1981 gccgcggcgc gcacgccggc gggaagcaga tgggtatcaa gttttgccat gacgcgacga
     2041 ctccgcttta ttttcgcgaa actaccagta taagaaacct gcggcccgcg ccggtcccgg
     2101 ccgaagcacg ggtgtggaag cgcgggtgag gcggaatgct cgcgggcgga cccaaagcga
     2161 catcgggaac ccaggcctac ctggtctttc ggagaattcg cggaacgagg cgcggcgtgt
     2221 gcaggcaata gctgcggtag gactcgccga aggccgcgct ccgggccgcc tcctccgccc
     2281 gcattcgcag tccataggca aggacggcgg tcagggccag cgtgagcaga cttgcgccgt
     2341 tggtcagcgc gaggccgagc cctgcccaga gcagcaaggc gccggcgcag gccgggtgtc
     2401 gcaggcgccg atacgggccc gcggcggccc attcgcaagg cttcgccgcg gtggcctcga
     2461 tggcacatcg ccgcagcgcg ctcatcgccc actggcgcaa tgcgcagccc gccagcatca
     2521 gggcgaggcc gagaaagaac cagacgagag act
//
LOCUS       DASUJG010000317         5957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1067565, whole genome shotgun sequence.
ACCESSION   DASUJG010000317 DASUJG010000000
VERSION     DASUJG010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5957
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067565"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..325
                     /locus_tag="VFX38_06800"
     CDS             71..325
                     /locus_tag="VFX38_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314593.1"
                     /translation="MKALDVYDAAMRRSGATGDLGDLPLIAITHGRPFPGPFATLEKY
                     WGAAQERLAALSTNGELIIAKNSNHMIQHDEPDVVVAAIR"
     gene            complement(593..3094)
                     /locus_tag="VFX38_06805"
     CDS             complement(593..3094)
                     /locus_tag="VFX38_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HEX5314594.1"
                     /translation="MKTIKDVASGWFGVEPIWRDAKHALRQLSRSPGFALAVIFTLAL
                     SIGANTAIFSVVNALLIKALPYAEPFRLGAIYGRTTGPQSFDQRRNLDGEQWERLRDE
                     VPSLLSAVYSPGTATVNLRAGSRAQPVQNGRVSARYFGVLGIEPLIGSGFSEDEDRPN
                     GPKAVVLTYYLWRNAFGGDPAVLGRAVLLKGELFTVVGVLPQGAVTPLGADVYTALQP
                     SREGEGRARNFSAVVRLRDGRTWQQADAELNRAWADSDWDRRFVAENPGAKLSYYLVP
                     LQAAQTDALRPEVLALMLAAGFILLIACANLAGLALVRTLRRGGEIATRLALGASTWR
                     IQRQLWIENLLLALAGGAGALGVGFIVLELLLRLLPRRFLPVDTVALDGRVLAFTLSL
                     SVLTCVLFGALPALAMRKTDLRISMGSRGVIRAGDVRVRKGLIAGEVGLTVVLLAGAG
                     LLIRTLIHLETLPPGFDAKGVITAKASLDDVRYSDPAALRKLLGESLGAVRAIPGVRN
                     AAVASTLPYERALIDGVGLGNGGRAGQSVSADEVFVTPEYFDTLRIPMFSGRAFEERD
                     GPGAQPVVIVNRAFARKFLPGLDPIGLYLTRLNPAGQKLLIVGVVGDTLLSSVAELNE
                     GSAPLTAEETIYLPYAQIADGGFLSRMHAFFQPSWIVRTSTPIAGLTAQMQRELSALD
                     SNLTFSGFYAIDDLMLKTLATQRVEVALLTTMASLALLLSALGIFALVANIVAQRTRE
                     FGIRIALGATMRNIMLHVARTGVSAAVVGGAAGLLISAGALRAMRSVVYGVGVYDATT
                     ILVVVLLLGLVIFVAAILPALRVGRIDPVETLRNE"
     gene            3573..3740
                     /locus_tag="VFX38_06810"
     CDS             3573..3740
                     /locus_tag="VFX38_06810"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5314595.1"
                     /translation="MRPCERLADPAKAHLPILTIALDVGYQSVAPFNQAFRDTRGCTP
                     SAYRRRHAKPS"
     gene            3855..4310
                     /locus_tag="VFX38_06815"
     CDS             3855..4310
                     /locus_tag="VFX38_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314596.1"
                     /translation="MLPLSRRRLTRRTTSSYALLHCTVAAAFSLLTPAMGAIPAQTAT
                     WPVTEGLPGGGRYSPLADINTANVDQLEQAWVYRYGADDYFNGSFPFDRGTSSETTPI
                     LVGDRLSWRWRARRARLSKGRLRDRPGPAEVHSGPICRTLWGRPALVRV"
     gene            complement(4419..4700)
                     /locus_tag="VFX38_06820"
     CDS             complement(4419..4700)
                     /locus_tag="VFX38_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314597.1"
                     /translation="MKFRNGLGLTLFAMSLSATAGAGVPMPTDMPEYIVTAMSPDSSE
                     IVLPAPVPPAPLAAQPIIDNAAVLKELRAEQAQAAMAVLAKVAGEEVGN"
     gene            complement(4942..5385)
                     /locus_tag="VFX38_06825"
     CDS             complement(4942..5385)
                     /locus_tag="VFX38_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005322089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX5314598.1"
                     /translation="MPKPRPDHDLFALLVADVFHAAGEMRREGEAIAKQAGQTQARWQ
                     LMSVISEGDWTVADAARRLGVARQGVQRIADELVAAGLARYAENPRHRRSSYLRLTEE
                     GGRTLSEINGIAKPRHLALRRRFSRSELEQTRDVLRRAVSALREQ"
     gene            5443..>5957
                     /locus_tag="VFX38_06830"
     CDS             5443..>5957
                     /locus_tag="VFX38_06830"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX5314599.1"
                     /translation="MGKIDLYRNVHKGQRARLFVLTVDIGRLDYTQPATVAGCMMRLR
                     AMIDELREHGVHEERFIHPLLHAHAPAVAEMLEADHASFDQRLAELEALAARLEAGVE
                     PLEVGSELYRTLCSTLSGYLAHLDYEETVAMPALWRACTPDALGGVILDFNASRGDAE
                     RLHDLHAQIGAL"
BASE COUNT         1140 a         1955 c         1837 g         1025 t
ORIGIN      
        1 ggatgagacc cgggcacgca tgggcgccgc cgcgctgtct cccgcgtttt ttcggcgcat
       61 gagagcggac atgaaggcgc tcgacgtcta cgacgccgcg atgcgccggt ccggagcgac
      121 cggcgatctt ggcgacctgc cgctgatcgc gataacgcac ggccgcccgt ttccgggtcc
      181 gttcgccacg ctcgagaaat actggggcgc tgcgcaggag cggctcgccg cgctgtcgac
      241 gaatggcgag ctcatcatcg cgaagaacag caatcacatg attcagcacg atgagccgga
      301 cgtcgtcgtc gccgcgatcc ggtgagtgta cgaagccgcg gctcgcaacg cgccgcttcg
      361 ggcgcgctcc gggccgccac ccgcatagtc gcgcgatcct aagatcttgc tgcatttctt
      421 tcctgagtgt cgaggcgttc ggccggcgac tacggaaagc ctcggctggc aggaacggaa
      481 gatcgacgcg gagacggggc tgccgctcat tcacttgtcg atgcgctaga gagccgttcg
      541 ccagcctgcc gagcgtcgag ttcatcgaga ctcatgacga ttggcggaac gctcattcgt
      601 tccgtagcgt ctcgactgga tcgatgcgcc cgacacgcag cgccggcaag atcgccgcaa
      661 cgaatatcac gagcccgaga agcagcacga caacgagaat cgtggtcgcg tcgtatacgc
      721 ctacaccgta gacgacgctg cgcatcgcgc gcaacgcgcc ggccgagatg aggaggcccg
      781 ctgcacctcc cacgaccgcc gcgctaacgc ctgtgcgcgc tacatggagc atgatattgc
      841 gcatcgtcgc gccgagcgca atgcggatgc cgaattcgcg cgttctctgg gcgacgatat
      901 tcgcaaccaa ggcaaagatc ccgagcgcgc tcagcaacag tgcgagcgac gccatcgtcg
      961 tcagcagggc gacctcgacc ctctgcgtcg caagcgtctt aagcatgagg tcgtctatcg
     1021 cataaaagcc ggagaacgtc aggttggagt ctagggccga gagttcccgc tgcatttgcg
     1081 ccgtcagacc tgcgataggg gtgctcgtgc gaacgatcca actaggctgg aaaaacgcgt
     1141 gcatgcggga gagaaatccg ccgtcggcga tctgggcgta aggcagatag atcgtctcct
     1201 cggcggtcag aggtgcgctt ccctcgttga gctccgcgac ggaggagagg agcgtatcgc
     1261 cgacgactcc gacgatgagg agcttctgcc cggccggatt caatcgcgta aggtagagtc
     1321 cgatgggatc gagccctggc aggaactttc gcgcaaaggc ccggttcacg atcacgacgg
     1381 gctgcgcgcc ggggccgtcc ctttcctcga atgcgcggcc cgagaacatg ggaatcctta
     1441 gggtatcgaa atactcgggc gtgacgaaca cttcgtccgc cgaaacgctc tgccctgcgc
     1501 ggccgccgtt gcccaaaccc acgccatcga tcaacgcgcg ctcgtaaggc agggtcgatg
     1561 cgacggccgc attccgcacg ccgggaatcg cgcgcacggc gccaaggctc tcaccgagca
     1621 gtttgcgcaa cgcggccgga tcgctgtagc ggacgtcgtc cagcgaggct ttcgcagtga
     1681 tgacgccctt ggcgtcgaag cccggcggca gcgtctccag atgtatgagc gtgcggatca
     1741 gcaggcccgc gcccgcgagc agcacgaccg tgagccccac ttcaccggcg atcagaccct
     1801 tccggactcg aacgtcgccc gcgcgaatca cgccgcggct gcccattgag atgcggagat
     1861 ccgtctttct cattgcgagc gccggcagtg caccgaacag cacgcaagtg aggacggaca
     1921 acgacagcgt aaacgccaag acgcggccgt cgagcgccac ggtgtctacg ggtagaaagc
     1981 gccggggcag gagccgcagc agcaattcga gcacaatgaa tccgacaccc aaagcgcccg
     2041 ctccccccgc gagcgcgagc agcaggttct cgatccagag ctgtctctgg atgcgccagg
     2101 tcgacgcgcc gagggcgagg cgcgtcgcga tctcgcctcc gcgcctcagc gtgcggacga
     2161 gcgcgagtcc tgcgagattc gcgcaggcga tgagcagaat gaatcccgcg gcgagcatga
     2221 gcgcgagcac ttcaggccga agcgcgtcgg tttgggcggc ttggagcggc accaggtaat
     2281 agctgagctt cgcgccgggg ttctccgcga caaagcgccg atcccagtca ctgtcggccc
     2341 aggcacgatt gagctcggca tcggcctgct gccaagtccg gccgtcgcga aggcggacca
     2401 cggccgagaa gttcctggcc ctgccctcgc cttcgcggct cggctgaagc gccgtgtaaa
     2461 cgtccgctcc gaggggggtc actgcgcctt ggggcagcac tccgactacc gtgaagagct
     2521 cgcccttgag cagaaccgct cgcccgagaa cagcaggatc tccgccgaag gcgttgcgcc
     2581 agagatagta ggtcagcacg acggccttcg gaccgttggg ccggtcctcg tcctcggaaa
     2641 agccgctgcc gatgagcggc tcgattccga gcactccgaa ataacgtgcc gaaacgcgtc
     2701 cgttctgaac aggttgcgcg cgcgagcctg cgcggagatt caccgtcgcg gtccctgggc
     2761 tgtacaccgc ggagagcaga gacggcactt cgtcccgcag cctctcccat tgctcgccgt
     2821 cgaggtttcg gcgttggtcg aaagattgcg ggcccgtggt tcgcccgtag atggcgccga
     2881 ggcggaaggg ctcggcgtaa ggcagcgctt tgatcaagag cgcgttcacg accgagaaga
     2941 tcgcagtgtt ggcgccgatc gagagcgcga gcgtgaaaat cacggccagg gcgaagcccg
     3001 ggctgcgcga caattgccgc agcgcatgct tggcatcacg ccagatcggc tccactccaa
     3061 accagcccga ggcgacatct ttgatggtct tcattcgtcc ctcagtgcag caatcgaatc
     3121 gacgcagctc cccgtaacgc aatgatcgtg ccacctccgc ggattgcgta aggttctgat
     3181 tcggctggct ccatgagcga ttacggaacg agtgaccgcc tgagacgtcc cgaaaccgga
     3241 cacgtcattt cgattccggg acgagctacg gtcagaggcg aacgcgctcg agcaacgaaa
     3301 agtagaccgg cacagccgct atcgtaagtt cttttcgcga ctaactcttc ggctgttgct
     3361 tgtcaattct acatttcgaa tgatcgcgcg attctggcct taggcgacga gtccgacgca
     3421 gtagcgaaaa gaaagcagag aagagacatc gaaaggtaat caatttcgcc cgattccggg
     3481 cgccaggaac ctcttggaca ctgccaccgc cagaattcgt tcttcgacac cgctaattat
     3541 tcgttgacca atacgacgct accgggtcat aaattcgacc ctgcgagcga cttgccgacc
     3601 cggccaaggc gcatcttccg atcctcacca tcgccctcga cgtcggctat caatcggtcg
     3661 caccgttcaa ccaggcgttc cgcgacacga ggggctgcac cccctccgct taccggcggc
     3721 ggcacgcaaa gccctcctga aaactcgaaa acctactcga ttcctgaaat ccgcgagcga
     3781 ctgcaggaat ccggaagatt ccccagggac gcactgctca cgataccggc cacctgtatc
     3841 gattggagcc caggatgcta cccctctctc gccgccgcct cacgaggcga actacatcgt
     3901 cctatgcact cctccattgc acggtcgccg ccgccttttc cttgctcacg ccggcgatgg
     3961 gcgccatccc ggcgcaaacg gcgacctggc cggtgaccga agggctgccc ggaggcggcc
     4021 gctactcccc gctcgcagac atcaataccg ccaacgtcga ccaactcgag caggcgtggg
     4081 tgtatcggta cggcgccgat gactacttca acggcagttt tcccttcgat cgcggcacgt
     4141 catccgagac cacgcccatt ctcgtgggcg accgtttgtc gtggcgctgg cgggcacgac
     4201 gggctcggct ctccaaaggg cgactacgtg atcgcccggg ccctgccgaa gtccactcgg
     4261 ggccgatttg tcgcactctg tgggggagac cggcgctcgt ccgggtgtga agctgatcag
     4321 aataaacgaa gcggcgacat tcgcttgaat gcgccgcagc cataggcgag cggcgcagac
     4381 cgcggcgagc gcctgttgcg gcgcccgccg cagtgtgtct aattcccaac ctcctcgccc
     4441 gcgaccttgg cgaggacggc catcgcagcc tgcgcctgtt cggcgcgcaa ttccttcagc
     4501 accgccgcgt tatcgattat cggctgcgct gcaagcggtg ccggcggaac cggcgccgga
     4561 agaactatct cgctcgagtc gggagacatg gcggtcacga tatattccgg catatccgta
     4621 ggcatcggga ccccggcgcc ggcggtagcc gacagcgaca tcgcaaagag ggtcaagccc
     4681 aatccgtttc ggaatttcat cgcattgctc ctgattcgag gatgctttcc acgctacgaa
     4741 ttgtgcgccg cgcaagcctc ggactgtatc gagaaggctg gagaatgcag tagttaggtc
     4801 ggcaagatga tctctctgtg aactgagtca caatcgcgct tgccggctat gtggcgcacg
     4861 gaagcgacgt gcccttgctc cctccgccac gccttgcgca atgcctgcat ctggtcgcgc
     4921 aagtatcctg gcccatcagc atcactgctc gcgcagtgcg gataccgctc ggcgcagtac
     4981 atcccgtgtc tgctcgagtt cacttcgaga gaagcggcgt cgcagcgcca ggtggcgcgg
     5041 cttcgcgatg ccgttgattt cactcagcgt gcgccctccc tcctccgtga ggcgcaagta
     5101 ggaagacctg cgatggcgag gattctccgc gtaccgcgca aggccggccg cgacaagctc
     5161 gtcggcgata cgctgcaccc cctgacgcgc gacgccgagc cgccgcgccg catccgcgac
     5221 cgtccaatcc ccctctgaaa tgacggacat cagctgccag cgcgcctgcg tttgcccagc
     5281 ctgctttgcg attgcctcgc cttcgcggcg catctcgccg gctgcatgaa aaacgtcggc
     5341 aacgagcagc gcgaaaagat cgtgatcggg tcgaggcttg ggcatattga caacttattg
     5401 tcattaagac aacatattgt cattgtaaac cggagcgtgg aaatgggcaa gatcgatctg
     5461 tatcgcaatg ttcacaaagg gcagcgcgcc aggctcttcg tcctgaccgt cgatatcggc
     5521 cgcctcgatt acacgcagcc cgcaacagtt gcgggctgca tgatgcggct gcgggccatg
     5581 atcgacgaac tgcgcgagca tggcgtgcac gaggagaggt tcatccatcc gctgcttcac
     5641 gcgcatgcac ccgccgtcgc cgagatgctc gaggccgatc acgcctcctt cgaccagcgc
     5701 cttgccgagc tcgaagccct ggccgcgcga ctcgaggccg gcgtcgaacc gctcgaggtc
     5761 ggcagcgagc tctaccgcac gctctgcagc actttgtcgg gctacctcgc ccatctcgac
     5821 tacgaggaga cggttgcgat gcccgcacta tggcgcgcgt gcacgccgga tgcgctcggc
     5881 ggcgtcattc tggatttcaa cgcctcgcgc ggcgatgcgg agcgcctaca cgaccttcac
     5941 gcgcaaatcg gcgcgct
//
LOCUS       DASUJG010000318         2806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_213179, whole genome shotgun sequence.
ACCESSION   DASUJG010000318 DASUJG010000000
VERSION     DASUJG010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213179"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..1373)
                     /locus_tag="VFX38_06835"
     CDS             complement(213..1373)
                     /locus_tag="VFX38_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5314600.1"
                     /translation="MALAAPDLGEELNLLRETVRRFADAEIAPRAAEIDKTNTFPRDL
                     WPKLGELGLLGLTVPETAGGAGLGYLAHVVAMEEISRASGSVGLSYGAHSNLCVNNLF
                     ANGSEAQRRKYLPKLISGEWVGALAMSEAGAGSDVVGSMSCRAEKKGDRWIANGAKMW
                     ITNGPDADVAIVYMRSADRGGAGSRSITPFLVEKGMKGFHAAQKLDKLGMRGSNTSEL
                     VFENCEIPAENVLGEVNQGVYVMMRGLDTERLVLSGGPIGLMAAALDAVLPYVRERKQ
                     FGRAIGEFEVMQAKLADMHVALATARAYSYDIARRFDAGEKLRADAAAVLLYAAESAV
                     KVTLEAVQALGGNGYINEYPTGRLLRDAKLYDIGAGTNEIRRMLIGRELFKA"
     gene            complement(1376..2473)
                     /locus_tag="VFX38_06840"
     CDS             complement(1376..2473)
                     /locus_tag="VFX38_06840"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX5314601.1"
                     /translation="MSALTEMGHEEVVFFHSRDLGLKAIVAIHDTTLGPALGGCRMLP
                     YKSEDEALYDVLRLSRGMTYKNAVAGLNLGGGKSVIIGDPKKDKSEAFFRAFGRFIES
                     LGGRYITAEDVNTSVDDMVQIFQETRHVTGIPAVFGGSGDPSPFTALGTFRGIEAAAE
                     HKYGSKELSKLSFAVQGAGHVGVHLIRMLRETGAKVYVSDPDADRIRHVVEKFGAEAL
                     KLDEIYDTDAKVFVPCALGGSVNPETLDKLHCEIVAGSANNQLDTDATGDELEKRGVL
                     YVPDYVINAGGVINVAIELQGYNKDRATHQVNRIFDICKHVFHIAEHEGLPTYKAADH
                     LAEKRIAEVGKVRRPYLPARRAPINGRHALS"
BASE COUNT          455 a          898 c          935 g          518 t
ORIGIN      
        1 ctccgcgcct taggccggcc tacatgctgc ctatattgta ggcccgcaca agcgcagcgt
       61 tgcgggcgaa tcgggatgcc ggcaacggcg ctccgcgcct tatgccggcc tacatgctgc
      121 ctatattgta ggcccgcata agcatagcgt tgcgggcatt cgggacgccg gcaacggcgc
      181 tccgcggcct atgccgaccc aagaaaactg actcaggcct tgaaaagctc gcggccaatc
      241 agcatccgac ggatttcgtt ggtgcccgca ccgatgtcgt aaagcttggc gtcgcgcagc
      301 agccgcccgg tagggtactc gttgatgtag ccgttgccgc cgagcgcctg cacggcttcg
      361 agcgtcacct tgaccgcgct ttccgcggcg tagagaagca ccgcggccgc gtcggcgcga
      421 agcttctcgc cggcatcgaa gcggcgcgcg atatcgtagc tgtaggcgcg ggcggtcgcg
      481 agcgcgacgt gcatgtcggc gagcttcgcc tgcatcactt cgaattcgcc gatcgcgcgc
      541 ccgaactgtt tgcgctcgcg cacatagggc agcacggcat cgagcgccgc cgccatgagc
      601 ccgatcgggc cgccggaaag cacgaggcgt tcggtatcga gcccgcgcat catcacgtac
      661 acgccctgat tgacttcgcc gagcacgttc tcggccggaa tctcgcagtt ctcgaacacc
      721 agctccgatg tgttcgagcc gcgcatcccg agcttgtcga gtttctgcgc ggcgtgaaag
      781 cccttcatgc ccttctcgac caggaacgga gtgatggatc gcgatccggc accgccacga
      841 tccgcgctgc gcatgtatac gatggcgacg tcggcgtcgg ggccgttggt gatccacatc
      901 ttcgcgccgt ttgcgatcca gcgatcgcct ttcttctcgg cgcggcagct catcgagccg
      961 accacgtcgg agccggcgcc cgcctcgctc atcgcgagcg cgccgaccca ttcgccggaa
     1021 atcagtttcg gcaagtattt gcggcgctgc gcttcgctgc cgttggcgaa gagattgttg
     1081 acgcagagat tcgaatgcgc gccgtaggac aggccgaccg accccgatgc gcgcgagatt
     1141 tcctccatcg cgacgacgtg cgcgagataa ccgagcccgg cgccgccggc ggtttcggga
     1201 acggtaagcc cgagaaggcc gagttcgccg agcttcggcc acagatcgcg cgggaaggtg
     1261 ttggtcttgt cgatctcggc cgcgcgcggc gcgatttcgg catcggcgaa gcggcgcacc
     1321 gtctcccgca ggagattcag ttcctcgccg agatcgggag ccgcgagcgc cacgctcagg
     1381 acagcgcgtg gcggccgttg atcggcgcgc ggcgcgccgg aagataggga cggcgcacct
     1441 tgccgacttc ggcgatgcgc ttctcggcga gatggtccgc cgccttgtag gtgggaagtc
     1501 cctcgtgttc ggcgatgtga aaaacgtgct tgcagatgtc gaagatgcgg ttgacctgat
     1561 gcgtcgcgcg atccttgttg tacccttgaa gttcgattgc gacgttgatc acgccgccgg
     1621 cgttgatgac gtagtcggga acgtagagta cgccgcgctt ctcgagttcg tcgccggtgg
     1681 cgtcggtgtc gagctgattg ttggcggagc cggcgacgat ctcgcaatgg agtttgtcga
     1741 gagtctcggg gttgaccgaa ccgccgagcg cgcaggggac gaagaccttg gcgtcggtgt
     1801 cgtagatttc gtcgagcttc agcgcctccg cgccaaactt ctcgaccacg tgccggatcc
     1861 gatcggcgtc gggatcgctc acgtagacct tggcacccgt ttcgcggagc atgcggatga
     1921 gatgcacccc gacgtggccc gcgccctgca cggcgaagga cagcttcgag agttccttgg
     1981 agccgtactt gtgttcggcc gcggcctcga tcccgcgaaa ggttccgagc gcggtgaagg
     2041 gcgaagggtc gccgctgccg ccgaacaccg ccgggatgcc ggtgacgtgc cgggtttcct
     2101 ggaagatctg caccatgtcg tccaccgacg tgttgacatc ctcggcggta atgtagcgtc
     2161 cgccgagcga ctcgatgaaa cggccgaatg cgcggaagaa ggcttcgctt ttgtcctttt
     2221 tcggatcgcc gatgatgacg gacttcccgc cgccgagatt gagtccggcc acggcattct
     2281 tgtaggtcat gccgcgcgac aggcgcagca cgtcgtagag cgcctcgtct tcgctcttgt
     2341 agggcagcat ccggcagccc ccgagcgcgg gcccgagcgt ggtgtcgtga atggcgacga
     2401 tggccttgag gccgaggtcg cgggagtgaa agaacacgac ttcctcgtgg cccatctcgg
     2461 tcagggcgga catggtattc atggaatctg gatctccacc tgggaatgcg gacctgcgtt
     2521 tcgcgcccgg gcgctgcctg cacccgtgtt tccgcgaaaa tttcgccgtc gggccgcgaa
     2581 acaggcgctt cgcgccccgc gcagcgggcc ttcggaaacc ggcacattat accttctggg
     2641 ccggccggcg ggccgcgacg ttctataatc acggcgagtc cagagtcgag gcaagccgat
     2701 ggcaagaccg gccgaggccg ttcgggccgg ggtccggcag gagccggagc gtccgctgcg
     2761 cttcgtgacc gcggcctcgc tcttcgacgg ccacgatgcc gcgatc
//
LOCUS       DASUJG010000319         2970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_457474, whole genome shotgun sequence.
ACCESSION   DASUJG010000319 DASUJG010000000
VERSION     DASUJG010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457474"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VFX38_06845"
     CDS             complement(<1..369)
                     /locus_tag="VFX38_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314602.1"
                     /translation="MKGAHLLTVILRPKIVLPILLAAALFAVAFKLGDLGTVLARVQA
                     IPLWAMALALAGAVVYLAFKLLQFRHLVTNLGLRPDWRRLMLAFCVGELSVTLPFGIF
                     AQNWILSVGSKEDFGRSSAAT"
     gene            complement(366..1655)
                     /locus_tag="VFX38_06850"
     CDS             complement(366..1655)
                     /locus_tag="VFX38_06850"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX5314603.1"
                     /translation="MTFPWLPDAALDPHPRLTWFTECCFDLAVLAFYRKEEILPPDYG
                     LAPGTLIVSNHQRDADVPILTTTLCQRRGLPIRWPLPFYAMREDLLRPGFLAEHLAHW
                     PRPLPALLRTISLAWFFRIVRGEPLRRLREFTLGETLSALLAAGFGDAQCEAVFNARG
                     RREIEAAIGALPPTLRDLDLRHRGKLRRGLWGLRRLRLETFRGLKPLFRRDIAAQLAR
                     FSSLLDAGRVVYFAPEGTISADGRFGRVRDGAWRIVRLAAAPPPILPLALSYDPLGPG
                     RLRVIVRVGAPMREYDTRERRNFEADLEKKIRALYPLNASHLLAWYLRRGADRFTTEQ
                     FALWFARARETLGSAGLALDPLLATRETQEFVGERLRWLGRKKLVVRDGNSWRKPPSA
                     DVSPGWRKPAAIIRYFDNALADHLAALAPGVERDIAP"
     gene            complement(1798..>2970)
                     /locus_tag="VFX38_06855"
     CDS             complement(1798..>2970)
                     /locus_tag="VFX38_06855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5314604.1"
                     /translation="YRGGAGERGHPARRHGPPMTILTAIALGLWAAVLVVPWQPWRCR
                     ERLEAEAEGAAASETDFTVLIPARNEASVIGETLAALAAAAPGAPVIVTDDESDDGTA
                     GIVGASGLQNLILVAGTPPPPGWAGKLWALEQGLARVHTQRVLLVDADIRLAPGMIAA
                     VQRKADEGYALVSVLAEPCWRGLATRLLLPAFVYFFKLLYPFALANRPRGPIAAAAGG
                     VILVEREVLASIGGFAAWHDAIIDDCALAARVKRAGQRTFLGLSRGAQSLRQQNLASI
                     AAMVTRSAWVQLHESPLLLLAATALLVLAFWVPPAALAFAGVPRWLGVAACAALAASY
                     LPVLLYYRRNPLAAILLPVVATAYLVLTWLSALRAATGTRSAWKGRRYRRKVRDEP"
BASE COUNT          558 a          963 c         1042 g          407 t
ORIGIN      
        1 ggtcgccgcg gaactgcgtc cgaaatcctc tttgctcccg accgagagaa tccagttctg
       61 ggcgaagatt ccgaagggca gggtcaccga cagctcaccc acgcagaagg cgagcatcag
      121 gcggcgccag tcgggacgca ggccgagatt ggtgacgaga tggcgaaact gcagcagctt
      181 gaaggcgaga taaacgacgg cgcccgcgag cgcgagcgcc atcgcccata gcggaatcgc
      241 ctggacgcgc gcgagcacgg tgccgagatc gccgagtttg aaggctacgg cgaaaagcgc
      301 ggccgcgagg agaatcggga gcacgatctt cggccgcagg atcacggtga ggagatgcgc
      361 tcccttcatg gcgcgatgtc ccgctcgacg ccgggcgcga gcgcggcgag atggtcggcg
      421 agggcgttgt cgaagtagcg aatgatcgcg gcgggtttgc gccagccggg agagacatcc
      481 gcgctcggag gttttcgcca ggaattaccg tcgcgtacga cgagcttttt tcgcccgagc
      541 cagcgcaatc gttcgccgac gaactcttgc gtctcgcggg tcgcgagaag gggatcgagt
      601 gccagacctg cggagccaag ggtttcgcgg gcccgtgcga accacagcgc aaactgctcc
      661 gtcgtgaacc gatccgctcc gcgccgcaga taccaggcga gaagatgact cgcgttcagc
      721 ggatagagcg cgcggatttt cttctccagg tccgcctcga agttccggcg ttcccgggta
      781 tcgtactccc gcatcggagc gccgacccgc acgatcacgc gcaggcgccc cgggccgagc
      841 ggatcgtagg aaagcgcgag cggcagaatc ggcggcggcg cggccgccag ccggacgata
      901 cgccaggctc cgtcgcgaac gcgcccgaaa cgcccgtccg cggaaatcgt tccctcgggc
      961 gcgaaataca cgacccgccc ggcgtcgagg agcgacgaaa agcgcgcgag ctgcgccgcg
     1021 atgtcgcggc ggaagagcgg tttcaagccg cgaaaagtct cgaggcgcag acggcgcagc
     1081 ccccagagac cgcggcgcag tttcccccgg tgccgcagat cgagatcgcg aagcgtcggc
     1141 ggcagcgcac cgatcgccgc ttcgatttcg cgccggccgc gggcgttgaa gacggcctcg
     1201 cattgcgcgt cgccgaatcc ggccgcgagg agcgcggaca gggtttctcc cagcgtgaac
     1261 tcgcgcaggc ggcgcagcgg ctcgccgcgc acgatgcgaa agaaccaggc gagcgaaatg
     1321 gttcggagga gcgccggcag cggtcgcggc caatgcgcga ggtgttcggc gagaaaaccg
     1381 gggcgcagca gatcctcgcg catcgcataa aagggcagcg gccagcggat cggcaggcct
     1441 cgccgttgac aaagcgtggt cgtgagaatc ggcacgtcgg cgtctcgctg atggttggag
     1501 acgatgagcg tgccgggcgc caagccataa tcgggtggaa gaatttcttc cttgcgatag
     1561 aacgcgagca ccgcgagatc gaagcagcac tcggtgaacc aggtgagccg cgggtgcgga
     1621 tcgagcgccg cgtcgggcaa ccatgggaac gtcacggctc cgatcccgtg acgtatgagg
     1681 aaaaccgcgt aggccggtat aaggctgtaa ggccgttgcc ggcatccggc gccgaattgc
     1741 cgggaacgct ccgcttatcc cggcctacga gaaaatcggg ttcaattttt tcatttctca
     1801 cggctcatcc ctcacttttc ttcggtagcg ccggcccttc cacgccgagc gcgtgccggt
     1861 cgcggcacgc agcgccgaga gccatgtcag gacgagatag gcggtcgcga cgacgggaag
     1921 caagatcgcg gcgagcggat tgcgccgata gtaaagaagg acgggcaggt aacttgcggc
     1981 gagcgccgcg caggccgcga cgccaagcca gcgcgggacg ccagcgaagg cgagcgccgc
     2041 cggcggcacc cagaaggcga gcacgagaag cgcggtcgcg gcgaggagca acagcggcga
     2101 ttcatgcagt tgcacccagg cgctgcgcgt gaccatcgcc gcgatcgagg cgaggttctg
     2161 ttgccgcagg ctttgtgcac cgcgcgagag tccgagaaac gtgcgctgtc ccgcacgctt
     2221 gacgcgagcg gcgagcgcac agtcgtcgat gatcgcatcg tgccaggccg caaagccgcc
     2281 gatgctcgca agcacctcgc gttcgaccag aatgacgccg ccggccgcgg cggcaatcgg
     2341 gccccgcgga cggttggcaa gcgcgaacgg atagagcagc ttgaagaaat acacgaaggc
     2401 cggcagcagc agccgcgtgg cgaggccgcg ccagcagggt tcggcgagca ccgagacgag
     2461 cgcgtagccc tcgtcggcct tgcgctggac tgcggcgatc atccccggcg cgaggcggat
     2521 gtccgcatcg acaagcagca cgcgttgcgt gtgcactcgc gcgagtccct gttcgagcgc
     2581 ccagagtttg cccgcccagc cgggcggcgg cggcgttccc gcgacaagga tgagattctg
     2641 gagcccgctc gcgccgacga tccccgccgt gccgtcgtcg gattcatcgt ctgtcacgat
     2701 caccggagcg ccgggcgcgg ccgcggcgag cgcggcgagc gtttcgccta tcaccgacgc
     2761 ctcgttgcgc gccggaatca aaacggtgaa atccgtttcg gatgctgcgg ctccttccgc
     2821 ctccgcctcc agccgctcgc gacagcgcca cggctgccac ggtacgacga ggacggccgc
     2881 ccacaagccc aatgcaatcg cggtgagaat cgtcatcggg gggccgtgcc ggcgcgccgg
     2941 atgacctcgc tcgccagcac cacctcggta
//
LOCUS       DASUJG010000320         2107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1313580, whole genome shotgun sequence.
ACCESSION   DASUJG010000320 DASUJG010000000
VERSION     DASUJG010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313580"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1092)
                     /locus_tag="VFX38_06860"
     CDS             complement(13..1092)
                     /locus_tag="VFX38_06860"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5314605.1"
                     /translation="MTTPRSAAAGKRPLDILGINETEEQLYRTLIARPGASVPELARG
                     LMLAPGRIQRLLEALEAKGLATHTPERPRRYLPASPDIAMEALVLRRQEDLQRARGVI
                     RELQEQSAATRRGGEQEQMVELVTSEEAERHIVEQMSRSARNEMVALVRPPMLISRLD
                     VEPDQDSYTQREAERRGVRSRSIVDSAFLALPGAVARVQSDMRAGEDVRVISWLPFKM
                     VATDRRLALIPLDLEQSASPVLLVRSPALLEALYVLFEILWERSSPIALADRASAQTD
                     AMTPPLPPGAQELLSLMAAGLNDKKIASELRISASTLNRRIGEIMKLLDARSRFQTGW
                     LAALRFRPGEQTAHPAETREPESSN"
     gene            complement(1198..1416)
                     /locus_tag="VFX38_06865"
     CDS             complement(1198..1416)
                     /locus_tag="VFX38_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020560342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEX5314606.1"
                     /translation="MREPLGPERHRALRRRLELLMAEHRTLDEEVAALSASNHENQLS
                     LQRLKKRKLQLRDEIERVRSALIPDLNA"
     gene            1527..1886
                     /gene="queD"
                     /locus_tag="VFX38_06870"
     CDS             1527..1886
                     /gene="queD"
                     /locus_tag="VFX38_06870"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009594137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEX5314607.1"
                     /translation="MDVFKRFQIEAAHRLPNVPPEHKCARLHGHSFRIEVRVSGPVDA
                     ENGWVMDFADIAAAFEPYFAMLDHHYLNEIAGLENPTSENLARWLWERLKPALPGLSA
                     VEVAETCNAGCVYRGDE"
BASE COUNT          336 a          693 c          693 g          385 t
ORIGIN      
        1 gacagatttc tgtcaattcg aggattccgg ctcgcgggtt tctgcgggat gcgccgtctg
       61 ctctccgggc cggaagcgca gggcggcgag ccagccggtc tgaaaccgtg agcgcgcatc
      121 gagcaatttc atgatctcgc cgatgcgccg gttgagggtg ctcgcggaaa tccgcagttc
      181 gctcgcgatt ttcttgtcgt tcagtcccgc ggccatgagc gagagcaact cctgcgcccc
      241 cggcggcaga ggcggcgtca tcgcgtcggt ctgcgcagag gcgcgatcgg cgagggcgat
      301 cgggctcgat cgctcccaca gaatttcgaa aagcacatag agcgcttcga gaagcgccgg
      361 cgagcgcacg agcagcaccg gactcgcgga ctgttcgaga tcgagcggaa tgagcgcgag
      421 ccgccgatcc gtcgccacca tcttgaaggg cagccaggag atgacgcgca cgtcctcgcc
      481 ggcccgcatg tcgctctgca cccgcgcaac cgcgccggga agcgcgagaa aggcggaatc
      541 cacgatgctg cgcgagcgca cgccgcgcct ctccgcttcc cgctgcgtgt agctgtcctg
      601 atcgggttcg acgtcgaggc gcgaaatcag catcggcggg cgcaccagcg cgaccatttc
      661 gttgcgggcg ctgcgcgaca tctgctccac gatgtggcgc tcggcctctt cgctggtcac
      721 gagttcgacc atctgctcct gctcgccgcc gcgccgcgtg gcggcactct gctcctgcag
      781 ttcgcggatg acgccgcggg cgcgctggag gtcttcctgg cggcgcagca ccagcgcttc
      841 catcgcgatg tccggggacg ccggcaggta acggcgcggc cgctcgggcg tgtgcgtcgc
      901 gagacctttc gcttcgagcg cttcgagcag gcgctggatc ctgccgggcg caagcatgag
      961 tccgcgggcc agttcgggca cgcttgcgcc ggggcgggcg ataagcgtcc gataaagctg
     1021 ctcttccgtt tcgttgatgc cgagaatatc gagcgggcgc ttcccggccg ccgcggatcg
     1081 tggtgtggtc atggcttcgc ctgcaacatg gaatggctgc ggatcgcagc cggcactgta
     1141 gcgcgaaccg cgtgcgcccg gccgggcacg ccggattctc ggtcgaccgg ggccggctca
     1201 ggcgttgaga tcggggatga gggcgcttcg aacccgctcg atttcgtccc gcagttgcag
     1261 tttgcgcttc ttcaggcgtt gcagcgaaag ctgattctcg tgattgctgg cggaaagcgc
     1321 ggcgacttct tcgtcgagcg tccgatgctc cgccatgaga agctcgagac ggcggcgcag
     1381 cgcgcggtgc cgctcgggtc ccagcggttc acgcatcctg ttcccctttg gaggccgtgc
     1441 ttccgccaag ttcactccat cctagtccct cccggcgcgg ctggcaagcg tgcgacaatg
     1501 gcgcggggaa ggccgtcacg gggccgatgg acgtattcaa gcgctttcag atcgaggccg
     1561 cccaccggtt gccgaacgtg ccgccggaac acaagtgcgc gcgtctgcat ggacattcgt
     1621 tccgcatcga agttcgggtg tccggcccgg tcgatgccga gaacggctgg gtgatggact
     1681 ttgccgacat cgccgccgcc ttcgagccgt attttgcgat gctcgatcat cattatctga
     1741 acgagatcgc ggggctcgag aatcccacca gcgaaaacct cgcccgctgg ctgtgggagc
     1801 ggctgaaacc cgcattgccg ggactgtccg ccgtcgaagt cgcagagacc tgcaacgccg
     1861 gctgcgtcta tcgaggcgac gaataatctc gctcttccga aggaggctgc gattaccacc
     1921 tcccccttcc gaaggcagct gcgcctacga ctccctttcc gaagaacgtc acgctaccac
     1981 ctcccccttc cgaacgcagc tgcgcctgct acctccccct tccgcaggaa aggggatcga
     2041 gggggatgtc ttttccgggc cattttgcga agacgctggt cgtttcgcaa tcgaagaaga
     2101 catcccc
//
LOCUS       DASUJG010000321         3646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1801483, whole genome shotgun sequence.
ACCESSION   DASUJG010000321 DASUJG010000000
VERSION     DASUJG010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1801483"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..1141
                     /gene="ligD"
                     /locus_tag="VFX38_06875"
     CDS             137..1141
                     /gene="ligD"
                     /locus_tag="VFX38_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019546366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX5314608.1"
                     /translation="MHELLASLEEDEAAKLRREAPPQWASPMLATLTENASSDPQWLY
                     ERKFDGIRCLAHSRSGTPILYSRNRRRLDGSYPELVEALAAQAIGRSILDGEVVAFEH
                     KRTSFARLQGRSGIHDAERARASGIAVYYYLFDLLYFDGYDLRNLPLRRRKAILRKVL
                     RFRDPLRYSAHRNRDGEAWRAEACRRGWEGLIAKKADSPYVGRRSPDWRKLKCVRQQE
                     FVIGGFTEPRGSRHGFGALLLGYYERGKLRCAGRVGSGFDELQLRKLHAQLLRQERRS
                     PAFVDERASRHGVHWVEPALVAEIAFTEWTRDGRLRHPRFLGLREDKEAREVRRERPT
                     "
     gene            1138..2031
                     /gene="ligD"
                     /locus_tag="VFX38_06880"
     CDS             1138..2031
                     /gene="ligD"
                     /locus_tag="VFX38_06880"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006912679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX5314609.1"
                     /translation="MRIGRREVELSNRDKILFPGDAITKGELIDYYRKIAPAMLPHLR
                     NRALTLHRFPDGIGSDGFYQQNVSGYFPDWIARAKLGKGPDKTEHAVCNDTASLVYLA
                     NQAAIALHVWPARIDRPDYPDRMIFDLDPADTGFETVRFAALLLFELLSEALDLRCFV
                     MTTGSRGLHVVVPLDRSSDFDSVRAFARELADRVVARAPDRLTTEQRKNKRRGRLFLD
                     TTRNAYGQTAVAPYSVRARPGAPIATPLEWKEVDDPRLASDRYRLGNMFRRLGRKTDP
                     WRSINRHAARLETRRKRLARI"
     gene            complement(2068..3135)
                     /locus_tag="VFX38_06885"
     CDS             complement(2068..3135)
                     /locus_tag="VFX38_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003983269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX5314610.1"
                     /translation="MISSSEANIRAGALRRAAHALRLLAPLVVLALSAPTFAAIHVRG
                     NALVGGAGRTVRLLGVNRSGAEYACIQGWGFMDGPTDAPSIAAIAAWNVNVVRLPLNE
                     DCWLGINGVDPRYGGAPYRHFVEAYVRRLHAARLAVILDLHWNAPGSEPATGQQKMAD
                     RSHAVAFWQSLARSFRRDGNVIFDVYNEPHDISWECWRDGCGPWVGMQTLVLAIRATG
                     ARQPIMLGGLAWANDLSQWLVWEPRDPLHQLVASFHLYNFNACANTACWDATVAPVAS
                     EVPVVTGELGENDCAHDFIDSYMQWADNAGVSYLGWTWDTWDCGSGPALISDYDGTPT
                     AFGEGFKAHLELLANPAAPSP"
     gene            complement(3240..>3646)
                     /locus_tag="VFX38_06890"
     CDS             complement(3240..>3646)
                     /locus_tag="VFX38_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase"
                     /protein_id="HEX5314611.1"
                     /translation="LGRVDDVIKVAGHRLGTKELESACLAVPGVAEAAVVGVADEVKG
                     ALPEVFISLDSGADEHEVAVAVNARIVKTIGKIARPRKVHVVPDMPKTRSGKIMRRIL
                     AALADGRDPGDTATLANPEVVETIARQHVKAL"
BASE COUNT          663 a         1211 c         1159 g          613 t
ORIGIN      
        1 gaacgcggga aggctcagtg gctgttgatc aagatggatg acgaggaagc cgaccggcgg
       61 cgcaatcccg tcaacaccga acccgagtcg gtcctgagcg gaaagaccat cgccgagatc
      121 gcgcgcggag ccgacaatgc atgagcttct cgcgagtctc gaggaggacg aagcggcaaa
      181 gttgcgccgc gaagcgccac cgcaatgggc atcgccgatg ctcgccacgc tcacggaaaa
      241 cgcgtcttcc gatccccagt ggctctacga gcgaaagttc gacggcattc gctgccttgc
      301 gcacagccgc tcggggactc caatcctcta ctcccgcaat cgccggcggc tcgacggcag
      361 ctaccccgaa ctggtcgagg cgctcgcagc gcaggcgatc ggccgctcca ttctcgacgg
      421 cgaggtcgtc gccttcgagc ataagcggac gagtttcgca cgcctgcagg gccgctccgg
      481 cattcatgac gcggaacgcg cacgagcaag cggaatcgcg gtgtattact acctcttcga
      541 tcttctctat ttcgacggct acgatctgcg gaatttgccg ctgcgccggc gcaaggccat
      601 cctgcgcaag gtgctgcgat tccgcgatcc cctgcgttac agcgcgcacc gcaaccgcga
      661 cggcgaggcc tggcgtgccg aggcctgccg ccgcggctgg gaaggtctga tcgcgaagaa
      721 ggccgacagc ccctacgtcg gcaggcgctc cccggattgg cgaaaactga aatgcgtgcg
      781 gcagcaggaa ttcgtgatcg gcggtttcac cgagcctcgg ggttcgcgcc acggtttcgg
      841 cgcacttctc ctcggctatt acgaacgcgg caagctgcgc tgcgcaggac gtgtcggctc
      901 cggcttcgac gaactacagt tgcgcaagct tcatgcgcaa ctgctgcgtc aggagcggcg
      961 cagcccggcc ttcgtcgatg agcgcgcttc ccgccacggc gtgcactggg tcgagccggc
     1021 cctggtcgcg gagatcgcgt tcacggaatg gacccgcgac ggaaggctgc gccatccgcg
     1081 gtttctcgga ttgcgggaag acaaggaggc gcgcgaggtg cggcgcgagc gtccaacatg
     1141 agaatcggcc ggcgcgaagt cgagctttcc aaccgcgaca agatcctttt tcccggcgac
     1201 gccatcacca agggcgagct gattgattac taccgcaaaa tcgcccccgc catgcttccg
     1261 cacctccgga atcgcgcact cacgctgcat cgtttcccgg acggtatcgg ctcggacggt
     1321 ttctatcaac agaacgtttc cgggtatttc cccgactgga tcgcgcgggc caagctcggc
     1381 aagggacccg acaagaccga gcatgcggtc tgcaacgata ccgcgagcct cgtctatctc
     1441 gcgaaccagg ccgcgattgc tctgcacgtc tggccggcgc gcatcgatcg tccggattat
     1501 ccggatcgca tgatcttcga tctggaccct gccgataccg gcttcgagac ggtgcgcttc
     1561 gcagcgcttc tcctatttga gttactgtcg gaagcactcg acctgcgttg ctttgtgatg
     1621 acgaccggtt cgcgcggttt gcacgtcgtc gtgccgctcg atcgaagcag cgatttcgac
     1681 agcgtgcgcg ccttcgcccg cgaactcgcc gaccgcgtcg tcgcccgcgc gcccgatcgg
     1741 ctgacgacgg agcagcgcaa gaacaagcgc cgagggcggc tgtttctcga caccacccgc
     1801 aacgcctacg gccagaccgc ggtcgcaccc tactctgtcc gcgcgagacc gggtgccccg
     1861 atcgctaccc cgctcgagtg gaaggaggtg gacgatcccc gtctcgcctc cgatcgctat
     1921 cggctcggca acatgtttcg ccggctgggc cggaaaacgg atccctggcg cagcatcaac
     1981 cgtcatgcag ccaggctcga aacgcgccgc aagcgattgg cgcggatctg agcgcggccg
     2041 ggaaagctgc gcaaatcccg acggcattca aggcgacggc gccgcggggt tcgcaaggag
     2101 ttcgaggtgc gccttgaatc cctcgccgaa ggcagtcggc gtcccgtcgt aatcggaaat
     2161 cagggccgga ccgctgccgc aatcccaggt gtcccaggtc cagccgagat aggacactcc
     2221 ggcgttatcg gcccactgca tgtagctgtc gatgaaatcg tgtgcgcagt cgttctcgcc
     2281 cagctctccc gtcacgaccg gcacctcgga cgccaccggc gcaaccgtcg catcccagca
     2341 ggcggtgttc gcgcaggcat tgaaattgta aagatgaaag gaagccacca gctggtgcaa
     2401 aggatcccgc ggttcccaga ccagccactg cgaaagatcg ttcgcccagg cgagtccgcc
     2461 cagcatgatc ggctgccgcg cgcccgtcgc gcgaatggcg agaaccagtg tctgcattcc
     2521 gacccagggg ccgcagccgt cacgccagca ctcccaggag atgtcgtgcg gttcgttgta
     2581 gacatcgaaa atcacgttgc cgtcgcgccg aaagctgcgt gcgagcgact gccagaatgc
     2641 taccgcgtgg ctgcggtccg ccatcttctg ctggcccgtc gcgggctcgc ttccgggggc
     2701 attccagtgc agatcgagga ttaccgcgag acgcgccgcg tgcagtcggc gcacgtaggc
     2761 ttcgacgaag tgacggtagg gggcgccacc gtagcgggga tcgacgccgt tgatgccgag
     2821 ccagcaatcc tcgttcaagg gcaggcgaac cacgttgaca ttccaggccg cgatcgcggc
     2881 aatggacggg gcatccgtcg gaccgtccat gaaaccccag ccctggatgc aggcatattc
     2941 ggcgccggag cgattcactc cgagcagtcg taccgtgcga ccggcgccgc cgaccagcgc
     3001 attgccgcgg acatggatgg cggcgaaggt cggagccgat agcgccaaga cgacaagcgg
     3061 agcaagaagc cgcagcgcat gagcggctcg ccgcagggcg ccggcacgga tattcgcttc
     3121 cgagctagag atcatgttct ttctccgaaa acctccccgg aaccgaagtc ccgcagccgg
     3181 gaaggaaggc cgcgtctgcg tttcgcggat gggtccacaa ggtttcgctc ctgcgggtct
     3241 tacagggcct tcacgtgttg gcgtgcgatg gtttccacca cttccggatt ggcgagcgtc
     3301 gccgtgtcgc cgggatcgcg cccgtcggca agtgcggcga gaatccgacg catgattttc
     3361 ccggaacgcg tcttcggcat gtcggggacg acgtggacct tgcgcggccg cgcgatcttg
     3421 ccgatggtct tcacgattcg ggcattcacc gcgacggcga cctcgtgttc gtccgcaccg
     3481 gaatcgagcg aaatgaaaac ctccggcagc gcgcccttga cctcgtcggc gacgccaacc
     3541 acggccgcct ccgcgactcc gggaaccgcg aggcaggccg attcgagttc tttggtcccg
     3601 agacggtggc cggccacctt gatgacgtcg tcgacacgcc cgagta
//
LOCUS       DASUJG010000322         2062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_90600, whole genome shotgun sequence.
ACCESSION   DASUJG010000322 DASUJG010000000
VERSION     DASUJG010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90600"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VFX38_06895"
     CDS             <1..843
                     /locus_tag="VFX38_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314612.1"
                     /translation="AAAAEARRKRRLAIGVFAASAVVSLVLALLPMGTRRPAVAPQPA
                     AADTRDGQIRELLLDDRTLVLVVPEDRRPLEISVPQDCTITLNGEQFLLLRELAPGDQ
                     AHVTLADDSSRQLTAEAIDVTRPEVLEGLFRSVDAKRSRFEIDVTPDGKSSEIRIFQV
                     SPDLKPRLNHDRDFAGRPVTFEMLKPGDRIVARYFDRGERREAVSVDAYRLVSLSGTL
                     QQLNLDEREITLSAAGGERKFPLAAECTVTLNGEFASPRDLAPGDQVKIEHDVEVVKI
                     EARR"
     gene            1237..>2062
                     /locus_tag="VFX38_06900"
     CDS             1237..>2062
                     /locus_tag="VFX38_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX5314613.1"
                     /translation="MAWRVVFSLRCIRCLAAAAAAFLAPLTIWAEEQAASPRVDLYGD
                     PLPEGAIARLGSTRFSNSVKSPIRACTYTPDGRQIASITSDGKLQFWDRSSGYETREL
                     EVDRPAPHEPRAPIDDRAFRRDVNIAIAPDGNLVAIAWWGRGRIEIRELRAGNSVASI
                     TTRRRSPARAPSLVFSSDGRRLIAVEAGQLEIFDLKGEPVGPPLPPRSRIAISQTRDV
                     MLTKDDVKITTTPTRLELRDGATGGLQRELDAVDGAELMSGIAINARATKFAAAFGK"
BASE COUNT          364 a          619 c          691 g          388 t
ORIGIN      
        1 gccgcggcgg ccgaggcgcg acgcaagcgg cggctcgcca tcggcgtttt cgcggccagc
       61 gccgtggtga gcctggtgtt ggcgttgctg cccatgggaa cgcgcaggcc cgccgttgcc
      121 ccgcagccgg cggccgccga cacccgcgac gggcaaattc gcgagttact gctcgacgat
      181 cggacgttgg tgctcgttgt gcctgaagac cgccgacctc tggagatttc ggttccgcaa
      241 gattgcacca tcacgctgaa cggcgagcag tttttgctgc tgcgcgagct cgcgccgggc
      301 gatcaggccc acgtgacgct tgccgacgat tcgtcacgcc aattgacggc ggaagccatc
      361 gacgtgacgc ggcccgaagt gctcgaggga ctatttcggt cggtcgacgc caaacgctcc
      421 cgcttcgaaa tcgacgtcac ccccgacggc aaaagctcgg agatccgcat cttccaggtg
      481 tcgcccgatc tcaagccgcg attgaatcac gatcgtgatt tcgcgggtcg gccggtgacg
      541 ttcgagatgc tgaagccggg cgatcggatt gtggcgcgtt acttcgatcg gggcgagcgg
      601 cgcgaggcgg tttcggtcga cgcttaccgg ctcgtttcgc tttccgggac gctccagcag
      661 ttgaacctcg atgaacggga gataaccctt tcggccgctg gcggagagcg gaagtttccc
      721 ttggcggcgg agtgtacggt gacgctcaac ggcgaattcg ccagcccgcg cgacttggcc
      781 ccgggagacc aggtgaagat tgagcacgac gtcgaggtgg tgaagatcga ggctcgccgg
      841 taggcgcgta ctttcgaggc ctgtagggaa cggactccgt tgatctggcc gggcgcgggg
      901 tccgccgtcg tcacggtcca cgcgctcgac gacggtccgc ccggtacggg cgatccggaa
      961 tcggtcaccg aaaccgtgca gccgggcatg ggaatatttt ccgaaatgcg agggaatttg
     1021 tttgcaatcg tgcctgatcg ggtgtaaggt acgcgagcga tgcagcggcg ccgcccggga
     1081 aaaaggcgct gaatcgtcct gggacggctt ccgctctgag cggctcattc gagccatccg
     1141 ccgccaagat gccgcggcgc caaggggcac gatggctcag cgctttgatg tgtgtccttc
     1201 gaaatcttcg caatctcagt atcgggaggc gagggcatgg cttggcgtgt ggttttttca
     1261 ctgcgctgta ttcgctgcct ggcggccgcg gccgccgcgt tcttggcgcc gctgacaatt
     1321 tgggccgagg aacaagccgc ctcgccgcgc gtcgatctct acggcgaccc actgcccgaa
     1381 ggagcgattg cgcggttggg ctcgacgcgc ttctcgaatt cggtgaagtc gcccatccga
     1441 gcctgtacgt atacgccgga cgggcgacag atcgcatcga taacttccga tggtaaactg
     1501 cagttctggg acaggagttc aggatacgaa acgcgcgaac tagaagttga taggccagcg
     1561 ccccatgagc cgcgtgcgcc catcgacgat cgtgcattca ggcgtgacgt taatatcgcc
     1621 atcgctccgg acggaaacct ggtggcaatt gcctggtggg gccgcgggcg catcgaaatt
     1681 cgcgagttga gggcgggcaa ttcggtggcg agtatcacca cgcgccgccg ttctcctgcc
     1741 agggcgccgt cgctcgtgtt ctcgtccgat ggtcgccggc tcattgccgt tgaggcgggc
     1801 caactagaaa tttttgatct gaagggcgag cccgtggggc cgccgctgcc gccgcgatcg
     1861 agaatagcga tttcacagac gcgggacgtc atgctcacca aagatgacgt taaaatcacc
     1921 acgacgccta cgcgcctcga actgagggac ggtgccaccg gcgggctgca gcgcgaatta
     1981 gacgcagttg atggtgctga gcttatgtcg ggcatcgcaa tcaacgcccg ggccaccaag
     2041 ttcgccgcgg cttttggaaa aa
//
LOCUS       DASUJG010000323         2260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_702227, whole genome shotgun sequence.
ACCESSION   DASUJG010000323 DASUJG010000000
VERSION     DASUJG010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702227"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2260)
                     /gene="polA"
                     /locus_tag="VFX38_06905"
     CDS             complement(<1..>2260)
                     /gene="polA"
                     /locus_tag="VFX38_06905"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX5314614.1"
                     /translation="RPTGAVYGVVNMLKKMLREDAPTHVAVVFDAPGRTFRDELFAAY
                     KANRPPMPEEMAAQIEPLLAVVEALGLALVREPGVEADDVIATLATRAAAAGYEVEIA
                     TGDKDMAQLVGPRVTLVDTMRGTRLDRAGVTAKFGVPPERIVDLLALMGDTSDNIPGI
                     PGVGPKTAAKWLGEYGDLAGVRAHAAEIPGKVGENLRANLAALDLSHELATVKAELAL
                     AVSPDDLVPKKPDDERLRALYGELEFRGWLAELGEDKAPAKTERKQERAYVCIADEAA
                     LAEWIAKLESAECFAFDTETTSLNYLDARIVGLSFAVAPGEAAYLPLAHSEAEPFDRD
                     VVLARLKPILEDPARGKLGHHVKYDAHALANHGIVLAGIRHDTLLESYVLDPTAGRHD
                     LDSLAARVLDEQTIHYETVAGKGKKQIGFADVPIAAATPYAAEDADVALRLHAALWPK
                     LGATGRLREVYETIELPLVPVLLAMERRGVLIDRVQLTAQSGELARGMIELQEAAYRA
                     AGTRFNLDSPAQLGEVLFEHLGLPVRQKTPKGQPSTAESVLEELAADHELPRIVLEYR
                     ALAKLRSTYTEKLPASVNPATGRVHTSYNQTGASTGRLASSDPNLQNIPVRTEEGRRI
                     RQAFIAPEGSVLLAADYSQIELRIMAHLSGDEGLRRAFAEGRDIHRATAAEVFGKSLE
                     DVSANERRAAKAINFGLIYGMSAFGLARRLDIAREEAAVYIERYFERYPGVRKYMEET
                     RRNAKENGYVETL"
BASE COUNT          356 a          750 c          742 g          412 t
ORIGIN      
        1 acaaggtctc gacatagccg ttttccttcg cgttgcggcg cgtttcttcc atgtatttgc
       61 gcacgccggg atagcgttca aaatagcgct cgatgtacac ggccgcctct tcgcgggcga
      121 tgtcgagccg ccgcgcgagc ccgaaggccg acatgccgta gatgagaccg aaattgatcg
      181 ccttcgccgc acgcctttcg tttgcgctca cgtcttcaag cgatttgccg aacacctcgg
      241 cggcggtcgc gcgatgaatg tcgcggcctt cggcaaaggc ccggcgcagg ccctcgtcgc
      301 cggaaagatg cgccatgatg cgcagctcga tctgcgaata gtcggcggcg aggagcaccg
      361 agccttccgg tgcgataaat gcctggcgga tgcgccgccc ctcctcggtg cgcacgggaa
      421 tgttctgcaa attgggatcg ctggaagcca gccggccggt cgaggcaccg gtctggttat
      481 aggaggtgtg gacgcgcccg gtcgcgggat tcacgctcgc cggaagtttt tccgtataag
      541 tggaacgcag cttggcgagc gcgcggtact cgagcacgat ccgcggcagt tcgtggtcgg
      601 cggccagttc ctcgagcacc gattccgccg tcgagggttg acccttcggc gtcttctgcc
      661 gcacgggaag gcccaggtgt tcgaagagca cttcgccgag ctgcgccggc gagtcgaggt
      721 tgaagcgcgt gccggcggcg cgatacgccg cttcctgcaa ttcgatcata ccgcgtgcca
      781 actccccgct ctgggccgtc aactgcacgc ggtctatcag cacgccgcgc cgctccatcg
      841 cgagcagcac cggcaccagc ggcagttcga tcgtctcgta cacctcgcgc agccggcccg
      901 ttgccccgag tttcggccac aacgccgcgt gcaggcgcag cgccacgtcc gcgtcttccg
      961 ccgcgtaggg cgttgcggcg gcaatcggca cgtcggcgaa tccgatttgc ttcttgccct
     1021 tgccggctac ggtctcgtag tgaatcgtct gctcgtcgag cacgcgcgcg gcgagtgagt
     1081 cgagatcgtg gcgcccggcg gtcggatcga gtacatagga ttcgagcagg gtgtcgtgac
     1141 gaatgcccgc gagcacgatg ccgtgattgg cgagcgcgtg cgcgtcgtat ttcacgtgat
     1201 gcccgagctt gccccgcgcc ggatcttcga gaatcggttt cagccttgcc agcaccacgt
     1261 cgcgatcaaa aggttcggcc tcgctatgcg cgagcggcag ataggcggct tcgccgggcg
     1321 caacggcgaa ggacaggccg acgatgcgcg cgtcgaggta attcaggctg gtggtttcgg
     1381 tatcgaaggc gaagcattcg gcggattcga gcttcgcgat ccattcggca agcgccgcct
     1441 catcggcgat gcagacataa gcgcgctcct gcttccgttc ggtcttcgcc ggcgccttgt
     1501 cttcgcccaa ttcggcgagc cagcccctga attcgagctc gccgtaaagc gcgcgcaggc
     1561 gttcgtcatc gggttttttc ggcacgagat cgtcgggact caccgcgagc gcgagctccg
     1621 ccttcacggt cgcgagttcg tgcgacagat cgagcgccgc gagattcgcg cgtagattct
     1681 cgcccacctt gccgggaatt tccgccgcgt gcgcgcgcac gccggcgaga tcgccgtatt
     1741 cgccgagcca tttcgcggcc gtcttcggcc cgacccccgg aatgccggga atgttgtcgg
     1801 aggtatcgcc catcaacgcg aggagatcca cgatgcgctc gggtggcacg ccgaacttgg
     1861 ccgtgacccc ggcccgatcg agccgcgtgc cgcgcatggt atccaccagc gtgacgcgcg
     1921 gaccgacgag ctgcgccatg tccttgtcgc cggtcgcgat ctccacttcg tagcccgcgg
     1981 cagcggcgcg ggtggcaagc gtcgcgatga catcgtcggc ctcgacgccc ggctcgcgaa
     2041 cgagggcgag acccagcgcc tcaacaaccg cgagcagggg ctcgatctgc gcggccatct
     2101 cctcgggcat cggcgggcga ttggccttgt aggcggcgaa cagctcgtcg cgaaaggttc
     2161 gccccggcgc atcgaacacg accgcgacat gcgtcggcgc gtcctcgcgc agcatcttct
     2221 tcagcatgtt cacgacgcca tagacggccc cggtgggtcg
//
LOCUS       DASUJG010000324         3955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_91995, whole genome shotgun sequence.
ACCESSION   DASUJG010000324 DASUJG010000000
VERSION     DASUJG010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91995"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VFX38_06910"
     CDS             <1..226
                     /locus_tag="VFX38_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314615.1"
                     /translation="EMLGERREPFAKFSAALRETPLAPERLAALREEAARSLAKQDAL
                     ETPGAESFEDYLARYYAGGGVSRAPSTAAS"
     gene            330..1268
                     /locus_tag="VFX38_06915"
     CDS             330..1268
                     /locus_tag="VFX38_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5314616.1"
                     /translation="MAPVLSIHAGVEPRIAAVLAAIERVVLGKPREIRLALVCLIAGG
                     HLLIEDIPGVGKTTLARTLARVLGLAFRRIQFTSDLLPADILGATIFEREHERFVFRP
                     GPLFTQVVLADEINRATPRTQSALLEAMQEGQVSVDGEARPLGRPFFVIATQNPQEQI
                     GTYPLPESQLDRFAMRLALGYPDATAERMLYQAEDRRDLLAQLAPVLDADGVLALQQA
                     ANGVRTAPALLDYVQALVGWTRHSGRCDPGLSPRAGRVLLALARAQALVCGRPAALPE
                     DVQAVLVPAVAHRLQRHGATGGGALARELLETVPIP"
     gene            1379..2293
                     /locus_tag="VFX38_06920"
     CDS             1379..2293
                     /locus_tag="VFX38_06920"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX5314617.1"
                     /translation="MRAPWPCRILRGQVFILPTRFGWLGVATTLVLLLLGLNYQNAPI
                     FLLAFVFAALGPVSMVVTHRHLQGLELGAPTVAPVFAGDPIRVRVPVVNASGRRRRAL
                     IAFADEAVGPALDLAPGADGCFELVLPARERGRHRLRGLGIASSEPFGAFRAWARFAG
                     ANLECIVYPRPASRAPLPGGDADKLGGMRAADQPEDFVGIAAYRPGDRPGQIAWRAYA
                     RGGEIERKEFGGLGASSRWFDFDSLPELLAELRLQVLARWVLDAERSGAVVYGLRLQA
                     FRLSPARGGEHLDACLRALALYPGPYDA"
     gene            2286..>3955
                     /locus_tag="VFX38_06925"
     CDS             2286..>3955
                     /locus_tag="VFX38_06925"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HEX5314618.1"
                     /translation="MPEAPAPAASVPQARDSLLLLAAATVPFFFTLPPWVAGVALALI
                     AWRLFALHRGRLPPGSLWLLALAALAFGVVVLAFRSFGGAEAGGAFLVLTAALKALES
                     YSSRDFRLLSVIAVLLVAADFLLLRSLPLALYSAAVIWVGAAVALRSQAPQARPWTLL
                     RRSGALLLAALPIAAALFLLFPRLPGPLFHFGPARSAAVSGLAPVLAPGSIAALAESD
                     EIAFRVKFDGVPPPPQERYFRGPVFTRYDGTRWLPASSGRGIGLFHPRGKPIRYRVLE
                     RSNGSRYLFALALPITVSTPARFTADYALLAPQQLWSDVAFRAVSYSSYTAGAMIAPA
                     ARAAALALPPGIDPRARALAARWQRESATPAALVARALAWFHDRPYYYTLSPPPLTGP
                     NRVDEFLFTTRRGFCEHYASAFAVLMRAAGIPARIVTGYAGGTVNTYDGWLILRQANA
                     HAWDEVWLAGRGWTRVDPTAVIPPGRVEASAFTAAASGAGAMADLYGPRGGFFGHLGD
                     LWDAASTAWMEYVVGYGAGLQKSLLQRLGLGGSTPLLLALAMIAGAFAG"
BASE COUNT          473 a         1451 c         1326 g          705 t
ORIGIN      
        1 cgaaatgctc ggcgagaggc gcgagccgtt cgcaaagttc tcggccgcgc tccgcgaaac
       61 cccgctcgct ccggagcgtc ttgcggcgct gcgcgaggag gccgcacggt cgctcgccaa
      121 acaggacgcg ctcgagacgc cgggcgccga gtccttcgaa gactatctcg cccgctatta
      181 cgccggcgga ggagtctcgc gcgcgccgag caccgcggct tcctgaactc ttccgcccgc
      241 ggcttccgcg actgcctgcc ggaggcgggc aaaaggcgga tcgaaggtgc gacaatgccc
      301 gcgagggttc cgccgcaacg ggagtcgcga tggccccagt gctcagcatt cacgccggcg
      361 tcgaaccgcg gattgcggcg gtgctcgccg cgatcgagcg cgtggtgctc ggcaagccgc
      421 gggagatccg cctcgccctc gtctgcctga tcgcgggcgg gcacctcctc atcgaggaca
      481 ttccgggagt ggggaagacc acgctcgccc gcacgctcgc ccgcgtcctc gggctcgcct
      541 tccggcgcat ccagttcacc agcgatctgc tgcccgccga cattctcggc gcgacgatat
      601 tcgaacgcga gcacgaacgc ttcgtcttcc gtccgggacc gctgttcacc caggtcgtgc
      661 tcgccgacga gatcaatcgc gcgacgccgc gcacccaaag cgcgctgctc gaagcgatgc
      721 aggagggaca ggtgagcgtc gacggcgagg cacgcccgct cggccgcccc ttcttcgtga
      781 tcgcgaccca gaatccgcag gagcagatcg ggacttatcc actgcccgaa tcgcagctcg
      841 atcgctttgc aatgcgactc gcgctcggct accccgatgc caccgccgaa cgcatgctgt
      901 atcaggcgga agatcgccgc gatctgctgg cgcaactggc gccggtgctc gatgccgacg
      961 gggttctcgc gctgcagcag gcggcgaacg gcgtgcgcac tgcgcctgcg ctgctcgact
     1021 acgtgcaggc gctggtcggc tggactcgcc attcggggcg ctgcgatccg ggactttcgc
     1081 ctcgggccgg gcgcgttctt ctcgctctcg cccgcgcgca ggcgctcgtc tgcgggcgcc
     1141 ccgcggccct gcccgaagac gtccaggcgg tgctcgtgcc ggcggtcgcc catcgccttc
     1201 aacgccacgg agcgacgggc ggcggcgcgc ttgcccgcga actcctcgag acggtgccga
     1261 ttccctgagc cgccgcgtgc tccgatccag gccatcgccg gcggctccgt cgatcgctgc
     1321 ggagccgccg ccgcgccgct ggcgccagtg gatggagcgc gcgggggaac gccgggggtt
     1381 gcgcgcgccc tggccctgcc gaattcttcg cgggcaggtg ttcatcctgc cgacgcgctt
     1441 tggctggctg ggcgtcgcca ccacgctggt gctgctgctg ctcggactca attaccagaa
     1501 cgcaccgatt ttcctgctgg ccttcgtgtt tgccgctctc ggcccggtca gcatggtcgt
     1561 gacccatcgg catctgcagg gactcgaact cggagcaccg acggtcgccc cggtcttcgc
     1621 cggcgatccg attcgggtgc gcgttccggt cgtcaatgcc tccggtcgcc gccggcgcgc
     1681 gctgatcgcc tttgcagacg aagccgtcgg ccccgcgctc gacctcgccc ccggagcgga
     1741 cggctgtttc gagctggtgc ttcccgcgcg cgagcgcggg cgtcatcggc tgcgaggtct
     1801 cgggatcgcg agttccgagc ccttcggtgc ctttcgtgcc tgggcacgct ttgcgggcgc
     1861 gaatctcgaa tgcatcgtct atccgcgccc ggcgagccgc gcaccgttgc ccggcggcga
     1921 cgccgacaaa ctcggaggca tgcgtgccgc cgatcaaccg gaggatttcg tcggaatcgc
     1981 cgcctaccgc cccggcgacc gcccgggaca gatagcctgg cgcgcctatg cccgcggcgg
     2041 cgaaatcgaa cgcaaggaat tcggcgggct gggggcgagc agccggtggt tcgacttcga
     2101 ctccctgccg gaactcctcg ccgagttgcg gttgcaggtt ctggcgcgtt gggtcctcga
     2161 tgcggagcgg agcggggcgg tggtctatgg cctgaggctc caggccttcc ggctctcgcc
     2221 ggcacgcggc ggcgagcatc tggacgcctg tctgcgcgcg cttgcgttgt atccgggacc
     2281 ctacgatgcc tgaggcgccg gctccggcgg catcggtccc gcaggcgcgc gattcgctcc
     2341 tcctgctcgc cgccgccacc gtaccgtttt tcttcacgct gccgccttgg gtggccggcg
     2401 tcgcccttgc cctcatcgcc tggcgtctgt tcgccctgca tcgcgggcgc ctgccgccgg
     2461 gatcgctgtg gctgctggca ctcgccgccc tcgccttcgg cgtcgtcgtc ctggcctttc
     2521 gcagcttcgg cggggccgag gccggcggcg ccttcctcgt cctgacggcg gcgctcaagg
     2581 cgctcgaaag ctacagctcg cgcgatttcc gtctgctctc ggtaatcgcg gttcttctcg
     2641 tcgccgccga ctttctgctc cttcgctccc tgccgcttgc cctgtattcg gctgcggtaa
     2701 tctgggtggg ggccgccgtc gcgttgcgct cgcaggcgcc gcaggcccgg ccgtggacgc
     2761 tgctgcgccg cagcggcgcc ctgctgctcg cggccctgcc gatcgcagcg gccctgtttc
     2821 tgctctttcc ccgcctgccg ggaccgctgt ttcatttcgg gccggcgcgc tcggccgccg
     2881 tgagcggcct cgctccggtg ctcgccccgg ggagcatcgc tgcgctcgcc gaatccgacg
     2941 agatcgcctt ccgggtcaag ttcgacggcg ttccgccgcc gccgcaggag cgctatttcc
     3001 gcgggccggt gttcacccgc tacgacggaa ctcgctggtt gcccgcttcg tccgggcggg
     3061 gcatcggcct ttttcatccg cgaggcaagc cgattcgcta ccgcgtcctc gaacgctcca
     3121 acggcagccg ctatctgttt gcgctcgcct tgcctatcac ggtaagcaca ccggcgcgtt
     3181 tcaccgcgga ctatgcgttg cttgcgccgc agcaactctg gagcgatgtc gccttccgcg
     3241 ccgtttccta ttcgagctat accgccggtg ccatgatcgc gcccgccgcg agggccgcgg
     3301 ccctcgcgct tccgcccggg atcgatccgc gcgcccgcgc tcttgcggcg cgctggcaac
     3361 gcgagagcgc cacgcccgcg gcgctcgtcg cccgcgcgct tgcctggttc cacgaccgcc
     3421 cgtattacta cacgctttcc ccgccaccgc taacgggacc gaaccgcgtg gacgagtttc
     3481 tgtttacgac gcgccgcggc ttttgcgaac attacgcgag cgcctttgcc gtgctgatgc
     3541 gtgccgccgg aattccggcc cgcatcgtca cgggctacgc cggcggtacc gtcaatactt
     3601 acgatggttg gctcatcctg cgccaggcga acgcgcacgc ctgggacgaa gtctggcttg
     3661 caggacgcgg ctggacacgc gtggacccga cggcggtgat tccaccgggc cgggtggagg
     3721 cttcggcctt caccgcggcg gcgagcggag cgggcgcgat ggccgatctg tacggccccc
     3781 ggggtggatt cttcggacat ctcggcgatc tctgggatgc ggcgagcacc gcctggatgg
     3841 agtacgtcgt cggctacgga gccggtcttc agaagagcct gctgcaacgc ctcggcctcg
     3901 gcggctcgac tccgcttctg ctcgcgcttg ccatgatcgc cggcgccttt gccgg
//
LOCUS       DASUJG010000325         2064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_949575, whole genome shotgun sequence.
ACCESSION   DASUJG010000325 DASUJG010000000
VERSION     DASUJG010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949575"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VFX38_06930"
     CDS             complement(<1..356)
                     /locus_tag="VFX38_06930"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HEX5314619.1"
                     /translation="MGTSPETTPTITDGYDFTSGSLCLDFVNTASTHEPVTGEDLHSY
                     AHIVEWAHQARLIDDQQVAALGVVAAEDHTAADAAYVGTLALRETIYRLITAAIRERP
                     ADDGDLAAFNVALTANL"
     gene            478..672
                     /locus_tag="VFX38_06935"
     CDS             478..672
                     /locus_tag="VFX38_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314620.1"
                     /translation="MSEGQQRRRERLVRAEVLRLLDAEGIGVRHSLASVLRRVAERID
                     PLALEPRLPHKAPDDPRPAS"
     gene            complement(687..1103)
                     /locus_tag="VFX38_06940"
     CDS             complement(687..1103)
                     /locus_tag="VFX38_06940"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5314621.1"
                     /translation="MIELNETIASAIDNAVANAVPVLVASSSAEGMPDIAYKGSVMVF
                     DNDHLAWWERAHGTTLANVRENGHACLYYVNRAERQSFKFFGTAEVIDSGPVRDQVMA
                     RTIQVELDRDPDRKGVAVMVRIDKVIEPGKEPMVRE"
     gene            complement(1116..1610)
                     /locus_tag="VFX38_06945"
     CDS             complement(1116..1610)
                     /locus_tag="VFX38_06945"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX5314622.1"
                     /translation="MDLYQGTIKKRDRRAFLPQRIDVETLRRILQAGRMTPSSKNAEP
                     NRFIVVDDEATRQQLAALSPIAGWLAGAPIIIVIVQTLEHPFDAGRAAQNMMLAAYND
                     GIGSCPAHLPEDRVAGLLGIPADLFPNRVIGFGVIDPARDRPPRAVARKRLPLEDLVH
                     TNHW"
     gene            1752..>2064
                     /locus_tag="VFX38_06950"
     CDS             1752..>2064
                     /locus_tag="VFX38_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020548727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5314623.1"
                     /translation="MKKRLALSMPLDGSTLPAQVAIAREAETLGYTDAWSLEVDGIDC
                     FGALSQLGAATQMRLGTAIANVYTRGPATLAMTAAGAAELAPGRFVLGIGSGSQPIVE
                     QW"
BASE COUNT          365 a          631 c          687 g          381 t
ORIGIN      
        1 aggttcgcgg tgagtgcgac gttgaatgcc gcaaggtcgc cgtcatccgc cgggcgctca
       61 cggatcgcgg ctgtgatgag ccgatagatg gtctctcgca gcgcgagcgt gcccacgtac
      121 gcggcatcgg cagcggtatg atcttcggcg gcgactacgc cgagcgcggc cacctgttgg
      181 tcatcgatga gccgggcctg gtgggcccac tcgacaatgt gtgcgtagct gtgaaggtct
      241 tcgcctgtca ccggttcgtg agtggatgcg gtattgacga agtcgaggca aaggctcccg
      301 gatgtgaaat cgtagccgtc ggtgatggtg ggtgtggttt ccggcgaggt acccatgata
      361 accagcaaac cccctttgac aggtgatgaa gtccggcgct aaagtaaaca cctgcgtatg
      421 gattatagca ggttagctgc acgaggcacc cctatgttgc aagaccctgg aggactcatg
      481 agcgagggac agcagcgccg acgcgagcgc ctcgtgcgag ccgaggtcct ccgcctgttg
      541 gatgccgaag gtattggtgt gcgccattcg ctcgcgtctg tgctgcggcg cgtagcagag
      601 cgaatcgacc cgctggcgct cgagccccgg ctgccgcaca aagccccgga cgatcctcgc
      661 cccgcgagct agcccacccc gccgcgctac tcgcgcacca tcggttcctt gcccggttcg
      721 atgaccttat cgatgcgcac catgacggcg acgcccttcc gatccgggtc ccggtcgagt
      781 tcaacctgga tcgtgcgggc catcacctgg tctcggacag gcccagagtc gatgacttcg
      841 gcggtcccga agaacttgaa tgactggcgc tccgcgcggt tcacgtagta gaggcaggca
      901 tggccatttt cgcgcacatt cgccagggtc gtgccgtggg cgcgttccca ccacgcgaga
      961 tggtcgttgt cgaacaccat cacgctgccc ttgtaggcga tgtcgggcat gccctctgcc
     1021 gaggaactgg cgacgagcac gggcacggcg ttcgcgacgg cgttgtcgat agctgacgct
     1081 atcgtttcgt tgagttctat cacgcggtaa gtctcctacc aatggttggt gtgcacgaga
     1141 tcttccaatg gcagccgctt tcgcgcgacc gcacgcggcg gacggtcacg cgccgggtcg
     1201 atcacgccga agccgatgac gcgattggga aacaagtcag ccggaatacc gagcagcccg
     1261 gcgacgcgat cctcgggcag atgcgcgggg cacgagccga tcccgtcgtt gtaggcggca
     1321 agcatcatgt tctgtgcggc gcgcccggca tcgaacgggt gctcaagcgt ctggacgatg
     1381 acgatgatga tcggcgcgcc cgcaagccac ccggcgatcg gactgagcgc cgcgagttgt
     1441 tggcgcgtcg cctcgtcgtc gacaacgatg aaccggttcg gctcggcgtt cttcgaggag
     1501 ggcgtcatgc ggccggcctg gaggatgcgg cgcagcgtct ctacatcgat tcgttggggg
     1561 aggaaggcgc gccggtcacg tttcttgata gtgccctggt agaggtccat ctggggattg
     1621 tactcagttg aaagctgcga gttgatagtt gacgggacct gaaaccgcga tccgcgtctg
     1681 taacccgcgg tgagacaccc agccaggtgg gcctcttgca actaacaacc aacaacttac
     1741 aactaacacc tatgaagaag cgcctcgccc tgtccatgcc gctcgatggt tccacgctgc
     1801 ccgcccaggt cgcgatcgcg cgggaggccg agacgctcgg gtataccgac gcgtggtcgc
     1861 ttgaggtaga cgggatcgac tgtttcgggg cgctctcgca actcggcgcg gcgacacaaa
     1921 tgcgcctggg cacggccatc gcgaacgtgt acacgcgcgg gccagcaaca ctcgcgatga
     1981 ccgccgcggg cgcagcagag ctcgcgccgg ggcggttcgt gctcggcatc ggctcgggtt
     2041 cgcagccgat tgtcgagcaa tgga
//
LOCUS       DASUJG010000326         7749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1803548, whole genome shotgun sequence.
ACCESSION   DASUJG010000326 DASUJG010000000
VERSION     DASUJG010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7749
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1803548"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VFX38_06955"
     CDS             <1..238
                     /locus_tag="VFX38_06955"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HEX5314624.1"
                     /translation="PIEPANVNELRDGSHGMIRTEVRSVHGDSHLGHVFDDGPADRGG
                     LRYCINSASLRFVPRDEMEAEGYGDYLDQVEDGQ"
     gene            235..738
                     /gene="msrA"
                     /locus_tag="VFX38_06960"
     CDS             235..738
                     /gene="msrA"
                     /locus_tag="VFX38_06960"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX5314625.1"
                     /translation="MSEERAVLAGGCFWGMQDLIRKQPGVISTRVGYTGGKVANATYR
                     NHEGHAEAIEILFDPAKTSYRRMLEFFFQIHDPTTRNRQGNDVGTSYRSAIFYTSDEQ
                     KRIAEDAIADLNASGLWPGKTVTEVVPAGDFWEAEPEHQDYLERYPNGYTCHFIRPDW
                     KLPHRNA"
     gene            898..1815
                     /locus_tag="VFX38_06965"
     CDS             898..1815
                     /locus_tag="VFX38_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX5314626.1"
                     /translation="MSWHKYKEPECVDKSGPIALVIRPRPRDLGGFEVGRVLPARERR
                     MIGPFIFLDHAGPAEFDPGAGADVRPHPHIGLATVTYLFEGAFLHRDSLGSEQLIEPG
                     AVNWMTAGRGIVHSERTPQSARVHGSRLHGLQSWVALPKDKEESAPSFQHCERENLPE
                     IVENGVSMRLVAGSAYGRTSPVVTLSPLFYLDAQMPRGSRFVLPAEYEDRALYALDAA
                     VTVNGVACKADALAVLRSGVEVEIVAEEATRLILLGGAPLDGERFIWWNLVSSSREGI
                     EAAKRDWAEGRFPKVPGDDKEFIPLPEGI"
     gene            1892..2251
                     /locus_tag="VFX38_06970"
     CDS             1892..2251
                     /locus_tag="VFX38_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEX5314627.1"
                     /translation="MELRIKRVYEKAANSDGRRVLLDRLWPRGIRKEDAHIHYWARDL
                     AVSAELRKWFGHDPARWPEFRKRYRAEIRANKDGIAALREKVGDAKRVTIVFGARDEE
                     HNNAVVLCEYLHEHPLD"
     gene            2232..6518
                     /locus_tag="VFX38_06975"
     CDS             2232..6518
                     /locus_tag="VFX38_06975"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5314628.1"
                     /translation="MSIRSTDSALAAFTPVVAGWFDETFGAPTAVQAAAWPAIASGRD
                     VLVAAPTGSGKTFAAFLWALDGLARAAERGALAPGCRVLYVSPLKALSNDIRRNLEEP
                     LAAIRSRLGPFADAIETAVRTGDTPASARERMRRRPPHILVTTPESLYLLLTSESGRR
                     LLAPVETLIVDELHAVAGSKRGAHLALSLERLAALVARPLQRIGLSATQRPIERMGRF
                     LVGAGREPPAIVDLGYARRLDLALEVPDSPLATVTSAEVWSEIYNRLATCVRESRSTL
                     VFVNTRRLAERAGRHLGERLGTDAVAVHHGSLSRAEREQAEARLKAGELPVLIATASL
                     ELGIDIGEIDLVCQLGSPRSIATLLQRVGRSGHRLDAVPRGRLFPLTREDLLECTALL
                     DAVAHDELEETAIGPGARDVLAQQTVAAVAGAGECALDDLYAVLTRAWPYRDLTREAF
                     TEIVEMLALGFTTRRGRRLAHLSFDRAGGVLHARRGARRFALENGGVIPDRFDYDVRL
                     LPENLAIGTVGEDFAFESLAGDIFQLGNRSYRVARVTQNTVFAEDANGAPPTIPFWVG
                     EAPGRSRELSAAVSRLYVTAENALAQGDGDRLGAQLARIPGVPETAAGALAEYLAESM
                     NALGCLPNEHRVILERFFDEVGDQHLVVHSPRGIRVNRAYGLALRKRFCRRFNFELQA
                     AADDNALVLSLGATHSFPLAEVADYVKARDVREVLTQAVLTAPMFPARWRWVGATALA
                     LPRRRAGKRVPAPFQRADAEDLIALVFPEQLACQDNIVGEREVPDHPLVAQTMRECLD
                     ELMDAEGLEQLLAAREAGAIEVVACDLPEASPLARGIANARPWAFLDGGAAEERRTRA
                     ITPERAGLWAQALTTPDAAAVAQIRAEAWPEMRTAEELADALVLLGFVAEAEGREANA
                     QPPFDELAAAGRAARMETSGAEPLWIAAERLPELLAVFPQAHLVPRIASPQTKPLDAD
                     EALRSLIRSRLEGLGPASADALARPLGLPPARIEAALAALEAGGGVMRGRFDPAVSGE
                     SWCDRHLAARLARLQLERRRSETRPVAPAAFQRFVLDWQGVGGEARGVEVLAAALERL
                     AGFALPAGLWESEILPARLGEYRREDLDALLAGGRYVWARLAPPTVAARTGSLAQVPL
                     AFVPRAALADWLTTAGSAPEAPNAAARAVEVALREGGALFWSDLREATGLLPSALEEA
                     LATLIRAGRASADHAPALRALMRPARRRRPDQLAGAGRFGLLFRAQARCEWTPERVEF
                     FARVLLARYGIIFRALARREALMPPWRELLRAYWRLEARGEIRGGRFIAALAGEQFAL
                     PEALVPLARAEREALSTLRVVSAADPANLSAVLLLAMELPSAARLIFANGRPLAAFAR
                     GRYQALTPEGEHRRGELEACLRGAPSVRAHPDTRRAGTRAHLRAVSIAGR"
     gene            6599..7105
                     /locus_tag="VFX38_06980"
     CDS             6599..7105
                     /locus_tag="VFX38_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013819951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HEX5314629.1"
                     /translation="MAISMYELSVPVFERALANLAHVLERGAAHAQTVKIDPSVLTQA
                     RLYPDMFPLTKQVQIATDLAARGAARLAGREPPSLPDTEKSFEELQARIEKARALVNE
                     VRAQELEGAESRTIEFKSGPGTKKLPAVSYLMDFVLPNIYFHAATAYDILRHNGVVLG
                     KSDYLGTI"
     gene            complement(7179..>7749)
                     /locus_tag="VFX38_06985"
     CDS             complement(7179..>7749)
                     /locus_tag="VFX38_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5314630.1"
                     /translation="VRKLHESKGVHFHLGQIATAVDATGVRLEHGLHIGADFVVVGIG
                     VRPRTKLAQTAGLRVENGVVVDEYLQTSAPNVYAAGDIARWPDPASGERIRVEHWVVA
                     QRQGQCAARNILGAREAYTVPPFFWSKHYDKSIRYVGHAQQWDRIEAEGDLASPDYEA
                     RFIAGDKTLAVATLGRDRANLRAEAAMEA"
BASE COUNT         1227 a         2551 c         2655 g         1316 t
ORIGIN      
        1 gccgatcgag cccgcgaatg tgaacgagtt gcgcgacggc tcgcacggca tgatccgcac
       61 cgaagtgcgc tcggtgcacg gcgacagtca tctcggccac gtcttcgacg acggccccgc
      121 ggatcgcggc gggctgcgtt actgcatcaa ctccgcctcc ctgcgtttcg tgccgcgcga
      181 cgagatggag gccgaagggt acggcgatta tctggatcag gtggaggacg gacaatgagc
      241 gaagaacgtg cggtgctggc cggcggttgc ttctggggca tgcaggattt gattcgcaag
      301 cagccgggcg tgatttcgac gcgggtcggc tacactggcg gcaaagtcgc gaatgcgacg
      361 tatcgcaatc acgaaggcca cgccgaggcg atcgaaatcc tcttcgatcc ggcgaagacc
      421 agttatcggc ggatgctgga gtttttcttc cagattcacg atccgacgac gcgcaaccgt
      481 cagggcaacg acgttggaac gagttatcgc tccgcaattt tctatacgag cgacgaacag
      541 aaacggattg ccgaagatgc catcgccgat ctgaacgcat ccggcctgtg gcccggcaag
      601 accgtgaccg aagtcgtgcc cgccggcgac ttctgggaag ccgagccgga acatcaggat
      661 tatctcgaac gttacccgaa tggttacacc tgtcacttca ttcggcccga ttggaaactc
      721 ccgcatcgga acgcctaacg taggccggga taagcgcagc gttcccggca tggacgtgga
      781 tgccggcaac ggccttcggc cttatgccgg cctacgggac tttggacaac ggccatgtcg
      841 ggtttccctg cgttcagtcc aagctgcgtt ggaaactgtc gaagcgaggt aacgacgatg
      901 agctggcata aatacaagga accggaatgc gtggacaaga gcgggccgat cgcactcgtc
      961 atccggccgc ggccgcgcga tctcggtgga ttcgaagtgg ggcgcgtcct tccggcgcgc
     1021 gagcggcgga tgatcggccc cttcattttt ctcgatcacg cagggccggc cgagttcgat
     1081 ccgggcgcgg gggccgacgt gcgcccgcat ccgcatatcg ggctggcgac cgtgacgtat
     1141 ctcttcgagg gtgcgtttct gcatcgcgac agtctcggat cggagcaact catcgaaccg
     1201 ggcgcggtca actggatgac ggcgggacgc ggcatcgtgc attcggagcg cacgccgcag
     1261 tccgcccgcg tgcacggcag tcgtctgcac ggtcttcagt cctgggtggc gttgccgaag
     1321 gacaaggaag aaagcgcgcc gagtttccag cattgcgagc gcgagaatct gccggagatc
     1381 gtggagaacg gagtttccat gcggctcgtg gcgggatcgg cctacgggcg aacgtctccc
     1441 gtcgtaacgc tttcgccact tttttatctc gatgcgcaaa tgccgcgcgg cagtcgcttc
     1501 gtactgcccg cggagtacga ggatcgtgcg ctctacgcgc ttgatgccgc ggttacggtc
     1561 aatggggtgg cctgcaaggc ggatgcgctc gccgtgctgc gcagcggggt cgaagtggaa
     1621 atcgtcgccg aagaggcgac ccgcttgatt cttctcggag gcgcgccgct ggacggcgag
     1681 cgctttatct ggtggaacct ggtgtcgagt tcgcgcgagg gtatcgaagc cgcgaaacgc
     1741 gactgggcgg agggacgttt cccgaaggtt cccggcgacg acaaggaatt cattccgctt
     1801 ccggaaggaa tctagcgcct cgcacgtttc ggcggcgtgg aatttccgcg ttttccgcga
     1861 tttctgcgcc ggttggagta tcatgaaacg catggaattg cggatcaaac gcgtctacga
     1921 gaaggccgcg aattcggacg gccgccgggt gctgctcgat cggctgtggc cgcgcggcat
     1981 caggaaggaa gacgcgcaca ttcactactg ggcgcgcgat ctcgcggtga gtgcggagct
     2041 gagaaagtgg ttcggccacg atcccgcccg ctggccggaa ttccgcaaac gctatcgcgc
     2101 cgaaatccgc gccaacaagg acgggatcgc ggcgctgcgc gagaaggtcg gggatgcgaa
     2161 acgcgtgacg atcgtattcg gcgcccgcga cgaggaacac aacaacgcgg tggtgctttg
     2221 cgaatatctg catgagcatc cgctcgactg attccgcgct tgcggcgttc acgccggtcg
     2281 tcgccggctg gttcgacgaa acctttggcg cgccgactgc ggtgcaggcc gcggcctggc
     2341 cggcgatcgc gagcggccgt gacgttctcg tcgccgcacc cacgggctcg ggcaagactt
     2401 tcgccgcgtt tctgtgggcg ctcgacggcc tcgcgcgtgc cgccgaacgc ggcgcgctgg
     2461 ctccgggctg ccgggtgctc tacgtctcgc cgctcaaggc gctctcgaac gacatccgcc
     2521 gcaatctcga agaaccgctc gccgcgatcc gttcgcgcct cgggcccttc gccgatgcga
     2581 tcgaaaccgc cgtacgcacc ggcgacacgc cggcgtcggc gcgcgaacgc atgcgccggc
     2641 gcccgccgca cattctcgtc accacgccgg aatctctgta tctgctgctg acttcggaga
     2701 gcgggcggcg cctcctcgcg ccggtcgaaa ccctgatcgt ggacgaattg cacgcggtcg
     2761 ccggcagcaa gcgcggcgcg catctcgcgc tttcgctcga acggctggcc gcgctcgtcg
     2821 cgcgcccgct gcaacgcatc ggcttgtcgg cgacgcagcg acccatcgaa cgcatgggac
     2881 ggtttctcgt cggcgcggga cgcgagccgc ccgcgatcgt ggacctcggc tatgcccgcc
     2941 ggctcgatct cgcgctcgaa gttcccgatt cgccgctcgc gaccgtgact tccgccgaag
     3001 tctggagcga aatttacaat cgtctggcga cgtgcgtgcg cgaatcgcgc tcgacgctgg
     3061 tgtttgtcaa cacccgccgg ctcgccgagc gcgccgggcg gcatctcggc gaacggctcg
     3121 gcacggacgc ggtcgcggtg catcacggca gcctttcgcg cgccgagcgc gagcaggccg
     3181 aggcgcgcct caaggcgggc gaactgccgg tgctgatcgc gaccgcctcg ctcgaactcg
     3241 gcatcgacat cggcgagatc gatcttgtct gccagctcgg ttcgccgcgt tccatcgcga
     3301 cgctcttgca gcgcgtcggc cgctccggcc atcgtctgga tgcggtgccg cgcggacgat
     3361 tgttcccgct gacgcgcgaa gatctgctcg aatgcactgc cttgctcgat gccgtcgcgc
     3421 atgacgaact ggaagaaacc gccattgggc cgggggcgcg ggacgttctc gcgcagcaga
     3481 ccgtcgccgc agtcgcgggc gccggcgaat gcgcgctcga cgatctctac gccgtgctca
     3541 cgcgcgcgtg gccgtaccgc gatctcacgc gcgaggcgtt cacggagatc gtcgaaatgc
     3601 tcgcgctcgg cttcacgacg cggcgcgggc ggcggctcgc gcatctgtcc ttcgatcgcg
     3661 cgggcggcgt gctgcatgcg cggcgcggcg cgcggcgttt tgcgctcgag aacggcggcg
     3721 tcattcccga ccgcttcgac tacgacgtgc gccttctgcc ggagaatctc gcgatcggca
     3781 cggtgggcga ggacttcgcc ttcgagtcgc tcgccggcga catctttcag cttggaaacc
     3841 gctcctatcg cgtcgcgcgc gtcacgcaga acacggtgtt cgccgaggat gccaacggcg
     3901 cgccgccgac gattccgttc tgggtcggcg aggcgccggg acgaagccgc gaactttcgg
     3961 ccgcggtctc gcgcctgtac gtcacggcgg agaacgcgct ggcgcaggga gatggcgacc
     4021 ggctcggggc gcaactcgcg cgcattcccg gcgttcccga gaccgccgcc ggcgcgctcg
     4081 ccgagtatct cgccgagagc atgaatgcgc tcggctgtct gccgaacgaa caccgcgtga
     4141 ttctcgaacg gttcttcgac gaagtcggcg atcagcatct ggtggtgcat tcgccgcgcg
     4201 gcattcgcgt caatcgcgcc tacgggctcg cgctgcgcaa acgattctgc cggcgcttca
     4261 acttcgagct tcaggccgcc gccgacgaca acgcgctcgt gctctcgctc ggcgcgaccc
     4321 acagctttcc gcttgccgaa gtcgccgact acgtgaaggc ccgcgacgtg cgcgaggtgc
     4381 tgacgcaggc cgtgctcacg gcgccgatgt ttccggcgcg ctggcgctgg gtcggcgcga
     4441 cggcgctcgc gcttccccgc cggcgcgcgg gaaaacgggt gcccgcgccg tttcagcgtg
     4501 ccgatgccga ggatctgatc gcgctggtgt ttccggagca gctcgcctgc caggacaaca
     4561 tcgtcggcga acgcgaggtg ccggatcacc cgctggtcgc acagacgatg cgcgaatgtc
     4621 tcgacgaact gatggatgcg gaagggctcg aacaattgct cgcggcacgc gaggcgggcg
     4681 cgatcgaagt cgttgcctgc gatcttcccg aagcatcgcc gcttgcgcgc ggcattgcga
     4741 acgcgcggcc ctgggccttt ctcgacggcg gggcggcgga ggagcggcgc acgcgcgcga
     4801 tcacgccgga gcgcgcgggg ctctgggcgc aggcgctcac gactccggac gcggcggcag
     4861 ttgcgcaaat ccgcgccgag gcctggccgg agatgcgcac ggcggaggaa ctcgccgatg
     4921 cgctggtcct actcggcttc gttgcggagg cggaagggcg cgaagcgaat gcgcagccgc
     4981 ccttcgacga actcgccgcc gccggtcgcg ccgcgcggat ggagacgtcc ggagccgagc
     5041 ccttgtggat tgcggccgaa cgtctgcccg aactgctcgc cgtgtttccg caggcgcatc
     5101 tcgtcccgcg gatcgcgtcg ccgcaaacga agccgctcga tgccgacgag gccctgcgat
     5161 ccctgatccg ctcgcgtctc gaaggactcg gcccggcgag cgcggacgcg cttgcgcgac
     5221 cgctcggtct gccgccggcg cgaatcgaag cggcgctcgc cgcactcgaa gcgggcggcg
     5281 gcgtgatgcg cgggcgtttc gatcccgcgg tgagcggcga gagctggtgc gatcgccatc
     5341 tcgccgcccg gctcgcaaga ctgcaactcg agcggcgccg cagcgaaacg cgccccgtcg
     5401 cgcccgccgc gttccagcga ttcgtgctcg attggcaggg tgtcggcgga gaagctcgcg
     5461 gcgtcgaggt actcgccgcc gcgctcgaac ggctcgctgg cttcgccttg ccggccggac
     5521 tctgggaaag cgaaatcctg cccgcgcggc ttggcgaata ccggcgcgaa gatctcgatg
     5581 cgcttctcgc cggcggacgc tacgtctggg cgcggctcgc accgccgacc gttgcggcgc
     5641 gcacgggaag tctggcgcag gtgccgctcg cattcgtgcc gcgcgcggcg ctcgcagatt
     5701 ggttgacgac cgcgggatcc gcgccggagg caccgaacgc cgcggcgcgc gcagtagagg
     5761 tagcgcttcg cgaaggcggc gcgctcttct ggtccgactt gcgcgaggcg accggacttc
     5821 tgccttcggc gctggaggag gcgctcgcga ctttgattcg cgcggggcgc gcgagcgccg
     5881 atcatgcgcc ggcgttgcgc gcgctcatgc gaccggcgcg gcggcggcgc ccggaccaac
     5941 tcgcgggcgc agggcgcttc ggcctgttgt tccgtgcgca agcgcgctgc gagtggactc
     6001 ccgagcgggt cgagtttttc gcccgcgtgt tgctcgcgcg ttacggcatc atttttcgcg
     6061 cgctcgctcg tcgcgaagcg ctgatgccgc cctggcgcga actgctgcgc gcgtattggc
     6121 ggctcgaagc ccgcggcgag atccgcggcg gacgtttcat cgccgcgctc gcgggagaac
     6181 agttcgcgct gcccgaagca cttgtgccgc ttgcccgcgc cgaacgcgaa gcgctcagca
     6241 ctcttcgcgt cgtatccgcc gccgatccgg caaacctgag cgccgtactg ctgctggcca
     6301 tggaattacc ctcggctgcg cgcctgatct ttgcgaacgg tcgcccgctc gcggcgtttg
     6361 cgcgcggccg ttaccaggca ctcacgccgg aaggcgaaca ccgccgcggg gaactggaag
     6421 cgtgtctgcg cggagcgcca tccgttcggg cgcatcccga cacacgacgg gcgggcacgc
     6481 gcgcacactt gcgcgccgtc tccatcgccg ggcgctgaat tcgaagcgga acgaagaagg
     6541 aaagcggtag aatgacgccg tgccgcagag cgttgcggcg ttccttggag aaaccaacat
     6601 ggcaatttcg atgtacgaat tgtcggtacc cgtcttcgag cgcgcgctcg ccaaccttgc
     6661 gcatgttctc gagcgcggtg cggcgcacgc gcagaccgta aagatcgatc cgtcggtttt
     6721 gacgcaggcg cgactgtacc ccgacatgtt cccgctcacg aagcaggtgc agatcgcaac
     6781 cgatctcgcc gcccgcggcg ctgcgcgcct cgccggtcgc gaaccgccgt cgctcccgga
     6841 taccgagaag agtttcgaag aactgcaggc aaggatcgaa aaggcccgcg cattggtgaa
     6901 cgaagtgcgg gcgcaggaac tcgaaggcgc cgagagccgc accatcgaat tcaagtcggg
     6961 cccggggacc aaaaaacttc cggcggtgag ctacctgatg gatttcgtgc tgccgaacat
     7021 ctactttcac gcggcgaccg cctacgacat tctgcgtcac aacggcgtcg tgctcggcaa
     7081 gagcgactat ctcggaacga tctgaaaccg aggcccattc cggggcgacg ttttgcgatg
     7141 aaggcgcggg ccttcgcaat cgcgtttgat ccggggttct aggcttccat cgccgcttcg
     7201 gcgcgcagat ttgcgcggtc gcgaccgagc gtcgcgaccg cgagagtctt gtcgccggca
     7261 atgaagcgcg cttcgtagtc gggcgatgcg aggtcgcctt cggcctcgat gcgatcccac
     7321 tgctgcgcgt gaccgacgta gcgaatgctc ttgtcgtaat gcttgctcca gaaaaacggc
     7381 ggcaccgtat aggcttcacg ggcgccgagg atattgcgcg ccgcgcattg accctgacgc
     7441 tgggcgacga cccagtgttc gacgcgaatg cgctcgccgc tcgcggggtc cggccagcgc
     7501 gcgatgtcgc cggccgcata gacgttcggt gcactcgttt gcagatattc atcgacgacg
     7561 acgccgtttt cgaccctgag accggcggtt tgggcgagtt tcgtgcgcgg gcgcacgccg
     7621 atgccgacga cgacgaaatc ggcgccgatg tgcaggccgt gttcgaggcg gacgccggtg
     7681 gcgtccaccg cggtcgcgat ttggccgaga tgaaagtgga cgcccttcga ttcgtgcagc
     7741 ttgcgcacg
//
LOCUS       DASUJG010000327         9052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_338081, whole genome shotgun sequence.
ACCESSION   DASUJG010000327 DASUJG010000000
VERSION     DASUJG010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9052
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338081"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="VFX38_06990"
     CDS             <1..821
                     /locus_tag="VFX38_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX5314631.1"
                     /translation="GCALPEKDDHGAPGARDIADRLIASLGSMLCYWYHWSHNGRMIE
                     VETDDDSVGGHFLHLLHGEEPSKEWVRAMHTSLILYAEHEFNASTFAARVIAGTGADM
                     YSAITGAIGALRGPKHGGANEVAFDIQSRYGTPDEAEADIRRRVEAKEIVIGFGHPVY
                     TVSDPRNKVIKQVAKELSEEAGDTKLFDVAERIESVMWDAKKMFPNLDWFSAVSYSSM
                     GVPTAMFTPLFVIARTTGWSAHVIEQRADGKIIRPSANYTGPEDRKFVPLDRRS"
     gene            1034..2482
                     /locus_tag="VFX38_06995"
     CDS             1034..2482
                     /locus_tag="VFX38_06995"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005300863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HEX5314632.1"
                     /translation="MSLSDSKSAERPAPEKVLTDIADYVLDYEIESDEAFGTARYDLM
                     DTLACGMLALNYPACTKLLGPVVPGATLAGGARVPGTDYELDPVQAAFNIGAMIRWLD
                     FNDTWLAAEWGHPSDNLGGILAVADYISRRNVAGGKAPLTMKAVLAAMIKAHEIQGVI
                     ALENSFNRVGLDHVLLVRVATTAVVTQMLGGTREQIIAAVSHAFIDGGALRTYRHAPN
                     AGSRKSWAAGDATARGVRLALIALTGEMGYPTALSAKTWGFYDVLFKGRPFEFERDYG
                     SYVMENVLFKISYPAEFHAQTAVECAMKLHAQVLDRIGEIERVVIETQEPGVRIIDKT
                     GPLNNPADRDHCIQYMTAVPLIFGRLTAEDYEDDVAADPRIDSLRAKMEVRENERFTN
                     DYYDPAKRYIGNAVQLFFRGGSHSDRIEVEVPIGHRRRRAEGIPLLIEKFENAIARRL
                     PEKQCEAIKEICASGKTLEATAVSDFMALWTV"
     gene            2495..2860
                     /locus_tag="VFX38_07000"
     CDS             2495..2860
                     /locus_tag="VFX38_07000"
                     /inference="COORDINATES: protein motif:HMM:NF019278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX5314633.1"
                     /translation="MNARAGQPEQAVVIKPYERPYANTIAAGAGETVVPDFDRHTDIA
                     GWVWCRARDGRAGWTPRAWLSRKGEEWRLDRNFDAIELTVHPGETLEVQFEESGFAWA
                     SKSNGEKGWVPIECLELAK"
     gene            3006..4982
                     /locus_tag="VFX38_07005"
     CDS             3006..4982
                     /locus_tag="VFX38_07005"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX5314634.1"
                     /translation="MKPIRFSVLLLFAALLFPVASHAAAKTFQLSDLQRIVSLSEAQI
                     SPDGREIAIVVDTRDWKTDEPKEEIDLVNVATGTRRALTWNRTGLDMPRWSPDGSRLA
                     FLADDEDGDQTQLFVMAMAGGDPRRITRNKQGIDSYSWSPDGKSIAFIAQDPPLREKA
                     NKAHNGAFRVTDGNFLLQEDVAPWQLWLVSAAGGKAKRLTEGRFSLDTEQGGATPPAW
                     SRDGRSIAFTKFPNSYWGPSFHSVIAAVNVASGKVTTLVNEPTSMDLKFSPAADDYAF
                     LRPRGGDQNNGYAVYVGTSGTNRDATQALARNFNDYLWLPGGKQLLLQGEYGTNSVFW
                     IQPLGAAARRLDLDGVQASSDFSIANNGAIAFIGTTATHPNELYVMRSTKDRPHQLTD
                     LNGFVDKLALGRPVSVHWTNDGFDEDGVLTYPVQYAEGTKYPLVLVIHGGPEGASTLG
                     FDPLAQLLAAAGFLVFQPNYRGSINLGDKYQHAIYRDTGDGPGKDVMSGLAAVEKLVT
                     VDRSRIGVTGWSYGGYMTAWLTGHYDVWKAAVAGAPLTDWVMDYTVSYYQRGDMYFFG
                     SSPWTDAGWKIWRQQSPITYARNVKAPTLLMGDVNDSNVPLVNAEEWYHALRDNGVTV
                     EFYAYPESSHFPGDIVQTTDVYRRWVGWMQKYLK"
     gene            4998..5510
                     /locus_tag="VFX38_07010"
     CDS             4998..5510
                     /locus_tag="VFX38_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011716490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HEX5314635.1"
                     /translation="MRHVFWLIPELLDGRAGPNREPWDIGELARAGFRAVLSVNDGEL
                     CHAEDFTRLGMAYACLPLSANAPPRRGDLQQCLDTLPEAYDFVSAQIGKRRPALIHCS
                     SGKDRTCLLMAYFLMRHDAVAPDDAVAQVRALRPIAFTAEGWESFAFAVLRESRKTLP
                     RSARTEPLDA"
     gene            5543..6085
                     /locus_tag="VFX38_07015"
     CDS             5543..6085
                     /locus_tag="VFX38_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013332840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEX5314636.1"
                     /translation="MYTYQDVLQFWFEETKPEQRFRKDAAFDALIRERFAATQRAAAQ
                     GELFEWRTTPGGRLAEIIVLDQFSRNLFRDDASAFACDGMALVLAQEAVAAGADRELA
                     SERRAFLYLPYMHSESPRIHEEAMRLFAEPGLEFNFEFERKHRAIIERFGRYPHRNAA
                     LGRDSTEEEIAFLREPGSGF"
     gene            6100..6747
                     /gene="rsmG"
                     /locus_tag="VFX38_07020"
     CDS             6100..6747
                     /gene="rsmG"
                     /locus_tag="VFX38_07020"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009573445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX5314637.1"
                     /translation="MKPDSSRPQARIAAGLDAVAIVPPSGAVEKLAAHLALVTEWNEA
                     CGLTAVRDADGMILRHVLDSAAALPFLRGRWMLDAGSGAGFPGIVLALLAPDTTWVLV
                     ESNGKKAGFLDHVLRRLGLGARVELYAGRLESYPGAPDFDTVTVRALANLARIGEWCE
                     DLLAPGGRLLAFKGRLREIERECAGLDANWRVAIEALRVPGLAAERHIVILERGS"
     gene            6750..7547
                     /locus_tag="VFX38_07025"
     CDS             6750..7547
                     /locus_tag="VFX38_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX5314638.1"
                     /translation="MARIIAVTNQKGGVGKTTTAVNLAAALAARDRRVLLVDLDPQAN
                     ATTGCGVDPRELAATTANLLLEEASVAAVIRPLRFEGLAFDLAPASADLTTAEVRLVS
                     EFGREHRLEKALQPVAEAYDFVFVDCPPALNLLTINALAAAAGVLIPMQCEYYAMEGL
                     SALADTIRRIRKSVNPAIVIEGILRTQFDARSNLTADVSAQLLLHFKDRVYRTVIPRN
                     VRLAEAPSFGVPVLLHDPASRGALAYQALAEEVLERAPASSPAPASG"
     gene            7603..8460
                     /locus_tag="VFX38_07030"
     CDS             7603..8460
                     /locus_tag="VFX38_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX5314639.1"
                     /translation="MTAQRRGLGRGLDALLSDVDAATETARSEDRLRELPVEQLERGR
                     FQPRKNLDAEALAGLADSIRAQGIVQPLVVRALAPERYEIIAGERRWRAAQLAGLATV
                     PAIVREVADRAAVAVALIENIQREDLNALEEAEALARLTEEFELTHAETAGAVGRSRA
                     AVTNLLRLLELGPEAKRLLREGRLAMGQARALLALGGSEQADAARKIAEGQLSTREAE
                     ALVRRLKSAGGKVPAAPPDPNVLALERELTERLGARVSLRAASGGKGRLIISYASLDE
                     LDGILARLR"
     gene            8502..>9052
                     /locus_tag="VFX38_07035"
     CDS             8502..>9052
                     /locus_tag="VFX38_07035"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEX5314640.1"
                     /translation="MKLPMVVALFAAAAALADTSVYRWVDASGVVHYSDQPQPGAVKV
                     DLGAPQVIDFKAPQALSATAPVTIVRPASSQVYELRILAPADGTTLRPVNNEVPARVA
                     VSPPLGIGARLQYLLDGQPLGPPTRATAMRLTQVYRGTHTLTVTVLAPDDEAAGTASS
                     TFFVHQHSALFRPRPGPKPPPGSG"
BASE COUNT         1598 a         2862 c         3033 g         1559 t
ORIGIN      
        1 tgggctgcgc cctgcccgaa aaagacgatc acggggcccc gggggcgcgc gatatcgccg
       61 atcgtctgat cgcctcgctc ggttccatgc tctgctactg gtatcactgg agtcacaacg
      121 ggcgcatgat cgaagtcgag accgacgacg attcggtggg cggccacttc ctgcatctgc
      181 tgcacggcga agaaccatcg aaggaatggg tgcgcgcgat gcatacctcg ctgattctct
      241 acgccgagca cgagttcaac gcctcgacct ttgctgcgcg cgtgattgcc ggcaccggcg
      301 cggacatgta ttccgccatc accggcgcga tcggcgcgct tcgcggcccg aaacacggcg
      361 gcgcgaacga ggttgcgttc gacattcaga gccgttacgg gacgccggac gaggcggaag
      421 ccgacatccg gcgccgcgtg gaggcgaagg aaattgtgat cggcttcggc catcccgtct
      481 ataccgtgag cgatccgcgc aacaaggtca tcaaacaggt ggcgaaggaa ctctcggaag
      541 aagccggtga cacgaagctg ttcgacgtcg ccgagcggat cgagtcggtc atgtgggatg
      601 cgaagaaaat gtttccgaac ctcgactggt tctcggccgt gtcctacagc tcgatgggcg
      661 tgccgaccgc aatgttcacg ccgctgttcg tgatcgcacg caccaccggc tggagcgcgc
      721 acgtgatcga gcagcgcgcg gacggcaaga tcatccggcc gagcgccaac tacaccggcc
      781 cggaggaccg caaattcgtc cctctcgatc ggcgctcctg acgtgatatc acgcccttcg
      841 gaacgaaggg gggcgcgccg aagacgcggg gggatggtct tgcgactggc aacgaagaca
      901 tccccctcaa tcccccttcg caagcgaagg gggagaagag tgtggcgcgt ctcaagcgaa
      961 gggggacgct ggaacccgcc gtcagaacga cggcgaaaga gattgctact tacgttggtt
     1021 acaggaatag cgaatgagtt tatccgacag taaatccgcc gagcgtcccg cccccgagaa
     1081 ggtgctgaca gacatcgccg actacgttct cgactatgag atcgagagcg acgaggcgtt
     1141 cgggacggcg cgctacgatc tcatggatac gcttgcctgc ggcatgctgg cgttgaacta
     1201 tccggcctgc acgaaactgc tcgggcccgt cgtgccgggc gcgacgctcg cgggcggcgc
     1261 gcgcgtgccg ggcacggatt atgaactcga tccggtgcag gcggccttca acatcggcgc
     1321 gatgatccgc tggctggact tcaacgacac ctggctcgcc gccgagtggg gtcatccgtc
     1381 cgacaatctg ggcggcatcc tcgccgtcgc cgattacata tcgcgccgca acgtcgccgg
     1441 tggcaaggca ccgctcacga tgaaagccgt gctcgccgcg atgatcaagg cgcacgaaat
     1501 tcagggcgta atcgcgctcg agaactcctt caatcgcgtc gggctcgatc acgtgctgct
     1561 ggtgcgggtc gcaaccaccg cggtcgtaac gcagatgctc ggcggcacgc gggagcagat
     1621 tatcgctgcg gtatcgcatg cgttcataga cggcggggcg ttgcgtacgt atcgtcatgc
     1681 gccgaatgcc ggctcgcgaa aaagctgggc cgcgggcgat gcgaccgcgc gcggcgttcg
     1741 cctcgcgctg atcgcgctca ccggcgagat ggggtatccg actgcgctct cggcaaaaac
     1801 ctggggtttt tacgacgtgc ttttcaaggg ccggccgttc gagttcgagc gcgactacgg
     1861 cagctacgtg atggaaaacg tgctgttcaa gatttcctat cctgcggagt tccatgcaca
     1921 gacggcggtc gaatgcgcga tgaagctgca tgcgcaggta ctggaccgga tcggcgagat
     1981 cgagagggtc gtgatcgaaa cgcaggagcc gggcgtgcgc atcatcgaca agaccgggcc
     2041 cctgaacaac cccgccgatc gcgaccactg catccagtac atgacggcgg ttccgctcat
     2101 cttcggccgc ctcacggcgg aagactatga agacgacgtg gccgccgatc cgcgcatcga
     2161 ctcgctgcga gcgaagatgg aagtgcgcga gaacgagcgc ttcacgaacg actactacga
     2221 ccccgcgaag cgctatatcg gcaacgccgt gcagctgttc tttcgcggtg gatcgcatag
     2281 cgatcgcatc gaagtggaag tcccgatcgg ccatcgccgg cgccgcgccg agggcattcc
     2341 cctgctgatc gagaaattcg aaaacgcgat cgcgcgccgc ctgccggaga aacaatgcga
     2401 ggctatcaag gagatttgtg cgagcggaaa aacgctggaa gccacagccg tctccgactt
     2461 catggcgctc tggacagttt gatgctctga catcgtgaac gctcgcgcag gtcaacccga
     2521 acaagccgtc gtgatcaagc cctacgagcg cccgtacgcg aatacgatcg cggcaggagc
     2581 cggcgagacg gtcgttcccg acttcgaccg tcacacggac atcgccggct gggtatggtg
     2641 ccgggcgcgc gacggtcgcg ccggctggac gccgcgggcc tggctgagtc gcaagggcga
     2701 ggaatggcgt ctggatcgca acttcgacgc gatcgaactg acggtccatc cgggcgagac
     2761 gctcgaagtc caattcgagg agagcggctt tgcctgggcg tcgaaatcga acggggagaa
     2821 gggctgggtt cccatcgagt gtctcgagct tgcaaaatga agctggcgtc aacaacttcg
     2881 tctgccggga caggagcagc gttcccggcg attcctgaca gcgggaaacg gccttatggg
     2941 ccttgtcggt ctacaatgac gcggtcgttg cgccttcccg aaattgtcgg atgaggagaa
     3001 agcttatgaa accgattcgg ttttccgtgt tgctcctgtt cgctgcgttg ctgtttccgg
     3061 tcgcgtctca cgcagccgca aagacattcc agttgagcga cttgcagagg atcgtgtcgc
     3121 tgagcgaggc gcagatttct ccggacggcc gggaaatcgc gatcgtagtc gacacgcggg
     3181 actggaaaac ggacgagccg aaggaagaaa tcgatctggt gaatgtcgcg accggcacgc
     3241 ggcgcgcgct gacctggaat cgcaccggtc tcgacatgcc gcgctggtcg ccggacggct
     3301 cgcggctcgc ctttctcgcc gatgacgagg acggcgacca gacccagctt ttcgtcatgg
     3361 cgatggcggg cggcgacccg cgccgcatta cccgtaacaa gcaggggata gacagctatt
     3421 cctggagtcc ggacggcaag agcattgctt tcatcgcgca agatccgccg ctccgggaaa
     3481 aggcgaacaa ggcgcacaac ggcgcgtttc gggtcaccga cggcaacttt ctgttgcaag
     3541 aggacgtcgc gccctggcaa ctgtggttgg tttccgcagc aggcggcaag gcgaagcgct
     3601 tgaccgaagg caggttcagc ctggataccg agcagggcgg ggcgacgccg cccgcctgga
     3661 gccgcgacgg ccggagcatc gcgttcacga agtttccgaa ttcctattgg gggccgtcgt
     3721 ttcattccgt gatcgccgct gtgaacgtcg cgagcggaaa agttaccacg ctcgtgaacg
     3781 aaccgacctc gatggatttg aagttctctc ccgccgcgga tgactatgcc ttcctgcgtc
     3841 cgcgcggtgg cgaccagaat aatggctatg cggtgtacgt cggcacgtcc ggcacgaatc
     3901 gtgatgctac gcaggccctt gcacgcaact tcaacgacta cctgtggttg ccgggcggaa
     3961 agcagttgct gcttcaggga gaatacggga cgaactcggt gttctggatc cagccgctcg
     4021 gcgctgctgc ccgacgcctc gatctcgacg gcgtgcaggc gtcttctgat ttcagcatcg
     4081 cgaacaatgg cgcaatcgcg tttatcggca ccaccgccac gcatccgaat gagttgtacg
     4141 tgatgcgttc gacgaaggac cggcctcatc agttgaccga tctcaacggc tttgtggaca
     4201 agctggcgct cggccgtccg gtatcggtcc actggaccaa cgatggcttt gacgaagacg
     4261 gcgtgctcac ctatcccgtg cagtacgccg aaggtacaaa gtatccgctg gtgctcgtca
     4321 tccacggcgg accggaaggc gcctccaccc tgggcttcga tccgctggcg caactgcttg
     4381 cggcggccgg ttttctggtg tttcagccga attaccgcgg gagcatcaat ctcggcgaca
     4441 aataccagca cgcgatttac cgcgataccg gcgacggccc cggcaaggac gtgatgtccg
     4501 ggctcgcggc ggtcgagaaa ctcgtaaccg tggaccggag ccggatcggc gtcaccggct
     4561 ggagctatgg cggctacatg acggcctggc ttaccggtca ttacgacgtg tggaaagccg
     4621 cggtcgcggg cgcgccgctc accgactggg tcatggacta caccgtgtcg tactatcaga
     4681 ggggcgacat gtatttcttc ggctcctcgc cgtggacgga cgcgggctgg aaaatctggc
     4741 gccaacaatc gccgatcacc tacgcgcgca acgtcaaggc gccgacgctc ctgatgggcg
     4801 atgtgaacga ctcgaacgtg ccgctcgtca acgccgagga gtggtatcac gcgctgcgcg
     4861 acaatggcgt gacggtggag ttttatgcct atcccgaaag cagccacttt cccggcgaca
     4921 tcgtgcagac caccgacgtc tatcgccgct gggtcggctg gatgcagaaa tacctgaaat
     4981 agtctgcgga atcttcgatg cgacatgttt tttggctgat cccggaactg cttgacgggc
     5041 gcgcgggccc gaatcgcgag ccttgggata tcggcgagtt ggctcgagcc ggctttcgcg
     5101 ccgtgctttc ggtgaacgac ggggagttgt gccatgcgga agacttcacg cggctgggca
     5161 tggcgtatgc gtgcctgccg ttgtcggcga acgcaccgcc ccgccgcggc gatctgcagc
     5221 aatgcctgga taccttgccc gaggcgtacg attttgtctc cgcgcagatc gggaaacgtc
     5281 gaccggcact gattcattgc tcgtcgggga aggaccgaac ctgcctgttg atggcctatt
     5341 ttctgatgcg tcacgacgcc gtcgcgcccg atgacgccgt ggcgcaggtc cgagcgctgc
     5401 ggcccatcgc gttcactgcg gaaggctggg agagcttcgc cttcgccgtg ttgcgcgaaa
     5461 gccggaagac cttgccccga tcggctcgca ccgagcctct cgacgcgtga gcgcgtcgtt
     5521 cgctttgcgt ggttgaatcg agatgtacac gtatcaggat gtactgcaat tctggttcga
     5581 ggagacaaag cccgaacaac gattccggaa ggacgcggcg ttcgatgcgc tgatccggga
     5641 gcgcttcgcg gcaacgcagc gcgcggcggc gcagggcgaa ctcttcgagt ggcgcacgac
     5701 gccgggcggg cggctggcgg aaatcatcgt gctcgaccag ttttcgcgca acctctttcg
     5761 cgacgacgca agcgccttcg cctgcgatgg catggcgctc gtgctggcgc aggaagcggt
     5821 cgctgccggt gcggaccgcg aactggcttc ggagcggcgc gcgttcctgt acttgccgta
     5881 catgcacagc gaatcgccgc gcatccacga agaagcgatg cgcctgttcg ctgagccggg
     5941 gctcgagttc aacttcgagt tcgagcgcaa gcaccgggca atcatcgagc gtttcgggcg
     6001 ttatccgcac cgcaatgcag cgcttgggcg tgactccacg gaagaggaga tcgcatttct
     6061 gcgcgagccc ggctccgggt tctgagcggg agcatgtcgg tgaagccgga ttcttcgcgt
     6121 ccgcaggcgc gcatcgcggc cgggctcgac gcagtcgcaa ttgttccgcc gtcgggcgca
     6181 gtggagaagc tcgcggcgca tctggccctg gtcaccgaat ggaacgaagc ttgcggtctg
     6241 accgcggtcc gcgatgccga cggcatgatc ctgcgccatg tgctcgactc ggccgcggcg
     6301 cttcccttcc tgcgcgggcg gtggatgctc gacgccggga gcggcgcggg atttcccggc
     6361 atcgtgctcg cgctgctggc gccggacacc acctgggtgc tcgtcgaatc caacgggaaa
     6421 aaggcgggtt tccttgatca tgtgctgcgg cgcctcgggc tcggggcgcg cgtcgagttg
     6481 tacgcgggaa ggctcgaaag ctatcccggc gccccggatt ttgataccgt cacggttcgg
     6541 gcgctcgcga atctcgcccg gatcggcgaa tggtgcgaag atttgctcgc tccgggcggc
     6601 cggcttctcg ccttcaaggg caggctgcgc gagatcgaac gtgaatgcgc cgggctcgat
     6661 gcgaattggc gggtggcgat cgaggcgctg cgggtgcccg ggctcgcggc cgaacggcac
     6721 atcgtgattc tcgaacgggg aagctgagga tggcgcggat catcgcggtc accaatcaga
     6781 agggcggtgt cggcaagacc acgaccgcgg tcaatctcgc cgccgcgctc gcggcgcggg
     6841 atcggcgcgt gctgctggtg gatctcgacc cgcaggcgaa cgcgaccacc ggctgcgggg
     6901 tggacccgcg cgaactcgcc gcgaccaccg ccaatctgct gctcgaggag gcgagcgtcg
     6961 ccgcggtaat ccggccgctc cgcttcgagg gtctcgcctt cgacctcgcg cccgcgagcg
     7021 cggatctcac caccgccgaa gtgcgtctgg tttccgaatt cgggcgcgag catcgtctgg
     7081 agaaggcgct gcagccggtg gcggaagcct acgactttgt cttcgtggat tgtccgccgg
     7141 cgctcaatct cctcaccatc aacgcgctcg cggcggccgc cggcgtgctg atcccgatgc
     7201 aatgcgaata ctacgcaatg gagggcctct cggcgctcgc cgacaccatt cgccgcatcc
     7261 gcaaaagcgt gaatccagcg atcgtcatcg agggaatcct gcgcacccaa ttcgatgcgc
     7321 ggagcaacct cacggccgac gtgtcggcgc agcttcttct gcatttcaag gatcgcgtct
     7381 accgcacggt gattccgcgc aacgtgcgtc tcgcggaggc gccgagtttc ggcgtgccgg
     7441 tgctcctcca cgaccccgct tcgcgaggcg cgctcgccta tcaggcgctc gccgaggaag
     7501 tgctggagcg cgcgccggct tcgtcgccgg cgccggcttc gggctgaacg gcttcgggcg
     7561 cgcggtagaa ttcccacgga ttctttgcgg agagcgaaag aagtgacggc acaacgccgg
     7621 ggtctcggcc ggggactgga tgcgctgctg agcgacgtgg atgcggccac ggagaccgct
     7681 cgcagcgagg atcggctgcg tgagctgccc gtcgagcagc tcgagcgcgg gcgcttccag
     7741 ccgcgcaaga acctcgatgc cgaggcgctc gcggggctcg ccgattccat ccgcgcgcag
     7801 ggcatcgtgc agccgctggt ggtgcgggcg ctcgccccgg agcgctacga gatcatcgct
     7861 ggcgagcgac gctggcgcgc ggcgcaactc gccggtctcg ccacggtgcc ggcgatcgtg
     7921 cgcgaggtag cggaccgggc cgcggtcgcc gtcgcgctca tcgagaacat tcagcgcgag
     7981 gacctcaatg cgctggaaga ggccgaggcc ctcgcgcggc tcaccgaaga gttcgaactc
     8041 acccacgcgg agacggcggg agccgtcggc cgctcgcgcg cggccgtcac caatctgctg
     8101 cgcctgctcg aactcgggcc cgaggccaag cgcctcctgc gcgagggcag gctcgcgatg
     8161 ggacaggcgc gggcgctgct cgcgctcggc ggctccgagc aggccgacgc cgcgcggaag
     8221 atcgccgagg gacagctttc gacccgcgaa gccgaggcgc tagttcgccg cctgaagtcg
     8281 gcgggcggga aggtgccggc ggcgccgccg gaccccaacg tgctcgcgct cgagcgcgaa
     8341 ctcacggagc ggctcggcgc gcgcgtgagc ctgcgcgccg caagtggcgg caagggccgg
     8401 ctgatcattt cctatgcgag tctcgatgaa ctcgacggca ttctcgcgcg cctgcgctga
     8461 ggcggccgca gccgcgtgcg ttccggcgta agctggaaag catgaaattg ccgatggtcg
     8521 tcgcgctatt cgccgccgcc gcggcacttg cggacacttc cgtgtaccgc tgggtggatg
     8581 cctccggcgt ggtgcactac tcggatcaac cgcagccggg tgcggtcaag gtggatctgg
     8641 gcgcacccca ggtcatcgac ttcaaggcgc cccaggcgct gagcgcgacg gcgccggtca
     8701 cgatcgtgcg ccccgcgtcc tctcaggtct acgaacttcg catcctcgct cccgccgacg
     8761 gcacgactct gcgcccggtg aacaacgaag tcccggcgcg cgtagcggta tcgccgcctc
     8821 tcggcatcgg cgcacgcctg cagtacctgc tcgacgggca gccgctgggc ccaccgacgc
     8881 gagcgaccgc gatgcgcctc acgcaggtct atcgcggaac gcacacgctc acggtaacgg
     8941 tgctcgcgcc cgacgacgag gcggccggaa cggcgagttc gaccttcttc gtgcaccagc
     9001 attcggcgct gttccggccg cggcccgggc cgaaaccgcc gccgggttcc gg
//
LOCUS       DASUJG010000328         5572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_460275, whole genome shotgun sequence.
ACCESSION   DASUJG010000328 DASUJG010000000
VERSION     DASUJG010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5572
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460275"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VFX38_07040"
     CDS             complement(<1..540)
                     /locus_tag="VFX38_07040"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEX5314641.1"
                     /translation="MNVANLAKDPVCGMTVSVASGTHSVDHQGRRFHFCSAGCRDRFV
                     ADPGRHVDDSPQPLPAASRGKWTCPMHPEIVRDAPGDCPICGMALEPLLPDVEANENP
                     ELRALSRRLWIAVPLTAALLFITMGDMLPGLDFAHWLGGAYGWVQFALTTPVMLYCGS
                     LFFRRGWRSLRGPNFNMWTL"
     gene            complement(537..875)
                     /locus_tag="VFX38_07045"
     CDS             complement(537..875)
                     /locus_tag="VFX38_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314642.1"
                     /translation="MKPPLVELVYFSGCPQVAAAREALRVALAAEGLPCRWREWDRDD
                     PAIPPPLRRCASPTILVNGRDVEPAVVDAAACRVFSQAAAPWDRSVREAIRAALRVAE
                     SKTNDPGDAE"
     gene            complement(872..1309)
                     /locus_tag="VFX38_07050"
     CDS             complement(872..1309)
                     /locus_tag="VFX38_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-responsive transcriptional
                     regulator"
                     /protein_id="HEX5314643.1"
                     /translation="MCNPCMVDPDNSLTVGALARRAGLSAEALRRYERLGLLSARRGR
                     SSNYRLYGPGAVRRLHFIQRAQSLGFSLEEIRELLSLHARAGASATEAKALADRRLKE
                     IEGRIRDLERMRAGLAALAHECDGEGPVGECPILAALAEAEPR"
     gene            1502..3238
                     /locus_tag="VFX38_07055"
     CDS             1502..3238
                     /locus_tag="VFX38_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314644.1"
                     /translation="MTTNRGTPGEHAVRLTVGVTAHRNLLDAEAAGIERLVGELFDGL
                     VRRFPDTPLQALCPLAEGGERIFARAARARGIPLIVPLPLPRDHYVEDFTSDASRREF
                     EDLCRDATVFELPLGPGVTAADVATSGPERDRSYAQLGVFVSSRCQILVAIWDGKGNG
                     QVGGTAQIVQYRLHNIYPARVFSRPLGQEVLADDENDLIFHIACSRDAPDGAPASPLR
                     ALESRWLTGADEAQAKRELPDTYARIFDMTAEFNRDCMHAAAPERARLGGIVAADGAW
                     PAGIRHLSRTFALADRLANHFQRRLNFALRSTYTLAVLMGAAFIGYTTVSAWRYLLYG
                     FFGLFALGFCLYEIARRRDWHRKYLDYRVLAEGLRVQCCWAIAGIGNSGDGRFVYENF
                     LQKQDPELGWIRQVMRDAELRTGFSDVNVQDGLDFVRRYWVGGAAAPAGQLGYYNREA
                     QRRYRLHRLTELLSSLCLWSGMIIAIVLGAVGERFSTTVVTILLLLLGLLPLIAAVRS
                     AYSHKRADKELIKQYRFMAHNFRNACRRLDDARDDEERREVLRALGLAALDEHAEWIL
                     LHRERPLERSRV"
     gene            3315..5486
                     /locus_tag="VFX38_07060"
     CDS             3315..5486
                     /locus_tag="VFX38_07060"
                     /inference="COORDINATES: protein motif:HMM:NF020509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEX5314645.1"
                     /translation="MSAEVKARRYRAFIAYAHRDRRWAAWLHRRLEFYRVPRRLVGRA
                     TPAGKVPRRLGPIFRDREELASSADLGESINAALDRSDALILVCSPAAARSRWVNEEV
                     ARFRRGRADSRIFCLIVAGDAASEECFPPALREDAQGGESPESGTAEPLAADLRREGD
                     GRKLALLKLVAGLLGVDLDELRRREAQRQVRRWSAVAVLAIAVTAATSLLAFEAMIQR
                     NAADRQRAQAEGLVSFMLGNLRNELEPIGRLDLLDMIGKRALAYYAAQNPDTLDADSL
                     ARRAKALLLIGQVYDQRGKLSDALPAFQEAARTTEKLLARKPNDAQRIYDHAQSVYWL
                     GYIAYQRGRTTKAKTEFGLYQTLAERLVAIDPDKAEWQTELEYASSNLGTLLLNENDS
                     IDAADAFHKALEVAERLVRKDPGNAQRQMDLAQSRAWLADSETHLGRFDDAGRQRRAE
                     LAIYDRLLTADPANTSVRESRVASQRALAAIAMAQGDLDGALKRAHGANADADYLVAR
                     DSSNADWQNLEAGALTNAAQILLYRGDISAASTAALRAEAIVRALTDRSPGVVVWQAV
                     LGQSRLLRARVAAGRDDHATALLIIRKSISGLVALPPKMREDEQIRELFLYSYLFEGD
                     QLHALGKSGSRAAWQAALRSLGRGDRRAPFDVRTAIAAACLRLGERARADAIVADLDA
                     SHYRAPDFLRLKRQIADSDAAAANPRSREGASKPHLQPEGN"
BASE COUNT          989 a         1892 c         1747 g          944 t
ORIGIN      
        1 cagggtccac atgttgaagt tcggaccgcg gagcgaacgc cagccgcgcc gaaagaaaag
       61 cgaaccgcaa tagagcatca ccggggtcgt gagcgcgaac tgcacccagc cgtatgcgcc
      121 tccgagccag tgcgcaaagt cgagccccgg cagcatgtcg cccatggtga tgaagagcag
      181 cgcggcggtg agcggaaccg caatccacag ccggcgggac aacgcgcgca gctccgggtt
      241 ttcgttcgct tccacgtccg gcaggagcgg ttcgagcgcc atgccgcaga tcggacaatc
      301 gccgggcgcg tcgcggacga tttcggggtg catcgggcag gtccatttcc cgcggctcgc
      361 tgcgggcaac ggctgcggcg aatcatcgac atgacgtccg ggatcggcga cgaagcgatc
      421 gcggcagccg gcgctgcaaa agtggaagcg tcggccctga tgatcgacac tatgcgtgcc
      481 gctcgcgacg cttaccgtca tcccgcacac cggatccttc gcgagattcg cgacgttcat
      541 tcggcgtccc cggggtcgtt cgtttttgac tccgcgactc gcagcgccgc gcgaatcgcc
      601 tccctcacgg agcgatccca cggcgccgcc gcctgtgaaa agacccggca ggcggccgca
      661 tccaccaccg ccggctcgac atcgcggcca ttgacgagaa tcgtcggcga ggcacagcga
      721 cgcagcggcg gcgggatcgc aggatcgtct cgatcccatt cgcgccaacg gcagggcagg
      781 ccctccgcgg ccagcgccac gcgcagggcc tcgcgcgcgg ccgcgacctg cgggcagccg
      841 gagaaataca cgagttccac cagcggcggc ttcatcgggg ctcggcctcc gccagcgccg
      901 cgagaatcgg acattcgccg acggggccct caccgtcgca ttcgtgcgcg agggccgcga
      961 gccccgcgcg catccgttcg aggtcgcgga ttctcccctc gatctccttc agcctcctgt
     1021 cggcgagtgc cttcgcttcg gtcgccgagg cgccggcccg cgcgtgcagg gacagcagtt
     1081 cgcgaatctc ttcgagcgaa aaacccaagg attgtgcccg ctggatgaag tgcaggcgcc
     1141 gcaccgcgcc aggtccgtag agacgatagt tggagctgcg cccgcgtctc gcgctcaaca
     1201 ggcccagacg ctcgtagcgg cgcagggcct cggcgctcaa tccggctcgc ctcgccagcg
     1261 cgccgacagt cagggaattg tcaggatcga ccatgcaggg attacacatt acagtagtgt
     1321 gtaatgtcaa gccccgccag cggatttggt caagaaggtt cgggctccca ggcttcgaaa
     1381 acgtcgagaa attctcccgc ccaacggcgg aagttgccgc tcgcttgctt tcgcgccgga
     1441 gccgttctag attgcagcgc ttcggcgccg gccggcatcg gtcaatccgg gatcgtcgtc
     1501 catgacaacc aacaggggaa ctcccggcga gcatgccgtg cgactgacgg tcggcgtgac
     1561 cgcgcatcgg aatctgctcg atgcggaggc ggccggcatc gaacgtctgg tcggagaact
     1621 cttcgacggg cttgtccggc gttttcccga cacaccgctg caggctctct gccctctcgc
     1681 cgaaggcggc gagcgcatct tcgcccgggc cgcgcgcgcg cgcggaattc cgttgattgt
     1741 ccctctccct ttgccacgcg atcactatgt agaagacttt acgagcgacg cgtcgcgacg
     1801 agaattcgaa gacctttgtc gcgatgcgac cgtattcgag ctgcctctgg gccccggggt
     1861 cacggcggcc gatgtcgcga cctcgggacc cgaacgcgat cgctcctacg cacaactggg
     1921 cgtattcgtt tccagccgct gtcagattct ggtggccatc tgggacggaa agggcaacgg
     1981 ccaggttggt ggcacagcgc aaatcgtgca gtaccgcctg cacaacatct atcccgcgcg
     2041 cgtgttcagc cgccccttgg gccaggaggt gctcgccgac gacgagaacg atctgatctt
     2101 ccacatcgcc tgttcgcgcg acgctcccga tggcgcaccg gcatcgccgc tgcgggcgct
     2161 cgagagcagg tggctgaccg gcgcggacga agcacaagcg aagcgcgaac ttcccgacac
     2221 ctacgcgcgc atcttcgaca tgaccgcgga attcaaccgc gactgcatgc acgcggccgc
     2281 gccggagcgc gcgcgcctcg gcggcatcgt cgccgcggat ggtgcctggc ccgcgggcat
     2341 tcgccatctc tcgcggacct tcgccctcgc cgatcggctc gccaatcatt ttcagcggcg
     2401 cctcaatttt gccctccgtt cgacctacac gctggcggtg ctgatgggcg ccgctttcat
     2461 cggctacacc accgtgagcg cctggcgcta tctcctgtac ggttttttcg gcctgttcgc
     2521 cctcggattc tgcctctacg agatcgcccg ccggcgcgac tggcatcgca aatatctcga
     2581 ctaccgcgtc ctcgccgaag gactgcgcgt gcaatgttgc tgggcgatcg cgggcatcgg
     2641 caacagcggc gacggccgtt tcgtttacga aaactttctc cagaaacagg atcccgaact
     2701 cggctggatc cgccaggtca tgcgcgatgc ggaattgcgc accggcttct ccgacgtcaa
     2761 cgtgcaagac ggcctcgatt tcgtgcggcg atactgggtc ggtggcgccg cggcacccgc
     2821 cggacaactc ggctattaca accgtgaagc gcaacgccgc tatcgacttc atcgcctgac
     2881 cgaactactt tcgagcctct gtttgtggag cggcatgatc atcgcaatcg tgctcggagc
     2941 cgtcggcgaa cgcttcagta ccacggtcgt gacgatcctg ctcctcctgc tcggactcct
     3001 gccgctcatc gcggccgtgc gcagcgccta ctcgcacaaa cgcgccgaca aggagttgat
     3061 caagcaatat cgcttcatgg cgcacaactt ccgcaatgcc tgccggcgcc tcgacgacgc
     3121 ccgcgacgac gaggaaagac gcgaggtgct gcgcgcgctc ggcttggcgg cgctcgacga
     3181 acatgctgaa tggattctcc tgcaccggga acggccgctc gagcgttcgc gcgtgtgagc
     3241 gcgcggaaaa agcctgaaaa ccgcgctata ctgcggcgcg attggttccc gagcgcctcc
     3301 ggcaggaggg taccgtgtcc gccgaagtaa aggcccgtcg atatcgcgcc ttcatcgcct
     3361 atgctcaccg cgatcgacgc tgggccgcct ggctgcatcg gcgcctggag ttttaccgcg
     3421 ttccccgccg cctggtggga cgtgcaacgc ccgcgggcaa ggttccacgc cgcctcgggc
     3481 cgattttccg cgatcgcgaa gaactcgcaa gcagtgccga tctcggcgaa agcatcaatg
     3541 ccgcactcga tcgctccgac gcactgatcc tcgtctgctc gccggcggcg gcgcgctcgc
     3601 gctgggtcaa cgaagaggta gcgcgcttcc ggcgcgggcg ggccgattcg cggatttttt
     3661 gtctgattgt tgccggcgat gccgcgtccg aagaatgttt cccgcctgcg ctccgagagg
     3721 atgcgcaagg cggcgaaagc ccggaatcgg gaaccgcaga gcccctggcc gcggacctgc
     3781 gcagggaagg cgacggccgc aagctcgcgc tgctgaaact cgtcgccgga cttctcggtg
     3841 tcgacctcga tgagttgcgc cgacgtgaag cgcaacgcca ggtgcgccgc tggtccgccg
     3901 tcgccgtgct cgcgatcgcc gtcacggccg cgacctcgct tctcgcgttc gaggcaatga
     3961 tccagcgcaa cgccgccgac aggcagcgcg cccaggccga aggtctcgta tccttcatgc
     4021 tcggcaatct gcgcaacgaa ctcgaaccga tcggccggct cgatcttctc gacatgattg
     4081 gcaagcgcgc actcgcctat tatgcggcgc agaatcccga cacactcgac gccgactctc
     4141 tcgcccggcg cgctaaggcc cttctgctga tcggccaggt ctatgaccag cgcgggaaat
     4201 tgagcgacgc ccttcccgca ttccaggaag ccgcgcgcac gaccgagaag ttgctcgcgc
     4261 gcaagccgaa cgatgcgcaa cgcatctacg atcatgcgca aagcgtctat tggctgggct
     4321 acattgcgta ccagcgcggc aggacgacga aggcgaaaac cgaattcggc ctgtaccaga
     4381 cgctcgccga gcggctcgtc gccattgatc cggacaaggc cgaatggcag acggaactcg
     4441 agtatgcgtc gagcaatctc ggcacgctgc ttctcaatga aaacgacagc atcgacgcag
     4501 ccgacgcctt tcacaaagct ctcgaagttg ccgagcgact ggtgcgcaag gatcccggaa
     4561 atgcgcagcg gcagatggat ctcgctcagt cgagagcctg gctcgcggac agcgagacgc
     4621 atttgggacg gttcgacgat gccggccggc aacgtcgggc tgaactcgcc atttatgatc
     4681 gcctgctcac ggcagatcct gccaatacca gcgtgcgcga atcgcgcgta gccagccagc
     4741 gtgcactcgc ggccatcgcg atggcgcaag gcgatcttga tggcgcactc aaacgcgctc
     4801 atggcgcaaa cgccgatgcg gactatctcg ttgcacgcga ttcgagcaat gcggattggc
     4861 aaaatttgga ggccggcgcg ctgacgaacg cggcgcaaat ccttctttac cgcggcgaca
     4921 tttccgccgc gtcgacggcg gcgttgcggg cggaggcgat cgtgcgcgca ctgacggatc
     4981 gctccccggg tgtagtggtc tggcaggcgg tactggggca gtctcgactg ctccgggcca
     5041 gggtcgccgc gggccgggac gaccacgcga cggctcttct catcatccgg aaaagcatct
     5101 cgggtcttgt tgcgttgccc ccgaagatgc gcgaagacga gcaaatccgc gaactattcc
     5161 tgtacagtta tctcttcgaa ggtgaccagt tacatgcgct cggaaaatcc ggaagccggg
     5221 ccgcctggca ggcggcgctt cggagcctcg ggcgcggcga ccgcagggct cccttcgacg
     5281 tgcgcaccgc gattgcggcc gcctgcctgc gtctcggcga aagggcgcgc gcggacgcca
     5341 tcgtcgcgga cctcgacgcc tcgcactatc gtgcgcccga ttttctccgt ttgaaacgcc
     5401 agatcgccga cagcgacgcc gctgccgcga acccccgctc gcgcgagggt gcctcgaagc
     5461 cccatcttca acccgaggga aactgaccat gaacgaagga tcaggccttg acgtcatctc
     5521 cattgacgtc acgctcgaac aaacgaacgg tcgcatcgcc ttccgctatc aa
//
LOCUS       DASUJG010000329         3371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1193637, whole genome shotgun sequence.
ACCESSION   DASUJG010000329 DASUJG010000000
VERSION     DASUJG010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193637"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3062
                     /gene="putA"
                     /locus_tag="VFX38_07065"
     CDS             <1..3062
                     /gene="putA"
                     /locus_tag="VFX38_07065"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEX5314646.1"
                     /translation="GRLIEETRLAPQVQSRVNSRARELVTAVRAKTSDQGGIEAFLRQ
                     YDLSSEEGIVLMCLAEALIRIPDADTADKLIRDKLARGAWSEKLGQSSSLFVNASTWG
                     LMVTGRLVRLDEGTESNPAALLARLVQRAGEPIVRASLKQAMRILGRQFVMGRSIKEA
                     LSRADDGDNRKFRYSFDMLGEAALTETDAKAYGDAYAAAIAAIGAARADRADIFSAHG
                     ISVKLSALYPRYELARRDDALGALVPRLQALAERARDCGIGLTVDAEEANRLDLSLDI
                     VEMVYRAAALADYEGFGLAVQAYQKRAPAVIDWLAELSGKVGRRIPVRLVKGAYWDSE
                     IKYAQIEGLEAYPVFTRKVNTDLCYLACAKRLLAAGAKLYPQFATHNAWTIAAIAELA
                     GDRRDYEFQRLHGMGEALYGEVLAGAGKGRHCRVYAPVGAHRELLPYLVRRLLENGAN
                     TSFVHRIGDARIPIEEIVADPVAAAGANERKAHPKIPLPRDLYLPERRNSRGINRADT
                     AALADLQQSMESALDGSWGAAPILGGKERDGRARSVPDPSDNRRILGEVADADAATVT
                     AAVTQAREAQPRWDATPAEERAAALERAADRLEQRMPEFMALCVREAGKSIPDSIAEV
                     REAVDHLRYNAAQCRRLFAEALALPGPTGERNRLELHGRGVFACISPWNFPIAIFLAQ
                     VSAALAAGNAVIAKPAEQTSLIGYRAVKLLHDAGVPGDVLQFLPGDGPGVGAPLVADP
                     RIAGVAFTGSTETARAINRVLAARDGPIATLIAETGGQNAMIVDSSALPEQVVLDVVG
                     SALLSAGQRCSALRVLCLQEEIAPRVLEILAGYMETLKVGDPGLLATDVGPVIDHDAR
                     KTLEAHAEAITKKGRVIARVPLPEACAHGSFVAPLAVEIEAIKVLEREVFGPILHVLR
                     YRSRDLEDTMRAVNATGYGLTFGVHSRIDGEAERLVREAAAGNCYVNRNMVGAVVGVQ
                     PFGGCGLSGTGPKAGGPHYLPRFASEKSVTVNTAAVGGNASLLAMEE"
     gene            3109..>3371
                     /locus_tag="VFX38_07070"
     CDS             3109..>3371
                     /locus_tag="VFX38_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEX5314647.1"
                     /translation="MQTSDFDYELPACLIAQHPSNERSASRLLVLDGASGAIEDRRMR
                     ELPDLLAAGDLLVVNDTRVIPARLLASKPSGGRVELLIERLTG"
BASE COUNT          572 a         1120 c         1124 g          555 t
ORIGIN      
        1 tcgggcgcct gatcgaggag acgcgcctcg cgccgcaagt gcagtcgcgc gtgaattcgc
       61 gggcacggga gctggtcacg gccgtgcgcg cgaagacatc ggatcagggc ggcatcgaag
      121 cctttctccg tcagtacgac ctttcttccg aggaaggcat cgtcctcatg tgcctcgccg
      181 aagccctgat ccggattccc gacgcagata ccgccgacaa gctgatccgc gacaagctcg
      241 cgcgcggtgc ctggtccgag aaactcgggc aaagcagttc gctcttcgtc aatgcctcga
      301 cctggggatt gatggtgacc gggcggctcg tccgcctgga cgagggcacg gaaagcaacc
      361 ccgcggcgct gctcgcacgt ctggtgcagc gcgcgggaga gccgatcgtg cgggcctcgc
      421 tcaagcaggc gatgcgcatt ctcggccgtc aattcgtcat ggggcgcagc atcaaggaag
      481 cactctcgcg cgcggacgat ggcgacaacc gcaaattccg ttacagcttc gacatgctgg
      541 gcgaggccgc gctcaccgag acggacgcga aggcctacgg cgacgcctat gctgccgcca
      601 tcgccgcgat cggagcggcg cgcgcggatc gtgcggatat tttttccgcg cacggcatct
      661 ccgtaaaatt atccgcgctc tatccccgct acgaattggc gcgccgcgac gacgcgctcg
      721 gcgcgctggt tccgcggctt caggcgctgg cggagcgcgc ccgcgattgc ggcatcggtc
      781 tcactgtcga tgccgaggaa gccaatcggc tcgatctgtc gctcgacatc gtcgagatgg
      841 tatatcgggc cgccgcgctc gcggattacg agggtttcgg ccttgcggta caggcctatc
      901 agaagcgcgc acccgccgta atcgactggc tcgcggaact ttccggcaag gtcgggcgcc
      961 gcattcccgt gcgtctcgtc aagggcgcct attgggacag cgaaatcaag tacgcgcaaa
     1021 tcgagggact cgaggcttac cccgttttca cgcgcaaggt gaacaccgat ctctgttatc
     1081 tcgcctgcgc gaagcggctc ctcgccgccg gcgcgaagct ctacccgcag ttcgcaacgc
     1141 acaacgcctg gaccattgcc gccatcgccg aactcgcagg cgatcgccgc gattatgaat
     1201 tccagcgttt gcacggcatg ggcgaggcgc tgtacggcga agtgctcgcc ggagccggca
     1261 aagggcgcca ttgccgcgtg tatgcgccgg tcggcgcgca tcgcgaactg cttccctatc
     1321 tcgtgcgccg gcttctcgaa aacggtgcga atacctcctt cgtgcatcgc atcggcgacg
     1381 cccggattcc gatcgaggag atcgtcgccg atcccgtggc ggcggcgggc gcgaacgaac
     1441 gcaaggcgca tccgaagatt ccgctgccgc gcgatctgta tctcccggaa cgccgcaatt
     1501 cgcgcggcat caaccgcgcg gatactgccg cgctcgcgga cctgcagcag tcgatggaat
     1561 cggcgcttga cggatcctgg ggcgcggctc cgatcctcgg cggcaaggaa cgggacgggc
     1621 gagcgcgatc cgttccggat ccgagcgaca atcgccgcat cctcggcgaa gtcgcggatg
     1681 ccgatgccgc aaccgtaacc gcggccgtca cacaggcgcg cgaggcgcaa ccacgatggg
     1741 atgcgacgcc ggcggaggaa cgggcggcgg cgctggagcg cgcggccgac aggctcgaac
     1801 aacgcatgcc ggaattcatg gcgctgtgcg tgcgcgaggc ggggaaaagc attcccgaca
     1861 gcatcgccga ggtgcgcgag gcggtggacc atctgcgcta caacgccgcg cagtgccgcc
     1921 gtcttttcgc ggaggcgctc gcgcttccgg ggccgaccgg cgagcgcaat cggctcgaac
     1981 tgcacggacg cggcgtgttc gcctgcatca gtccgtggaa ttttccgatt gcgatttttc
     2041 tcgcgcaagt gagcgctgcg ctcgcggccg gaaacgccgt catcgcgaag cccgcggaac
     2101 agacttccct catcggctat cgcgcggtga agttgttgca cgacgcgggc gttccgggcg
     2161 atgtcctgca attccttccc ggcgacggtc cgggagtcgg cgcgccgctg gtcgccgatc
     2221 cgcgcattgc aggagtcgcc ttcaccggct cgaccgagac cgcgcgcgcg atcaatcgcg
     2281 tgctagcggc gcgcgacggg ccgatcgcga cgctcattgc cgaaaccggc ggccaaaacg
     2341 cgatgatcgt ggacagctcc gcgcttcccg aacaggtcgt gctcgatgtc gtcggttccg
     2401 ctctgctgag cgcggggcag cgctgctcgg ccttgcgcgt gctctgcctg caggaggaaa
     2461 tcgcgccgcg ggtactcgag attctcgccg gctacatgga aacgctgaag gttggcgatc
     2521 ccggactgct cgcgaccgac gtcgggccgg tcatcgacca cgacgcgcgc aaaaccctcg
     2581 aggcgcatgc cgaggcgatc acgaagaagg gaagggtgat cgcgcgcgtg ccgcttcccg
     2641 aggcctgcgc gcacggaagt tttgtcgcgc cgctcgccgt cgaaatcgaa gcgatcaaag
     2701 ttctcgagag ggaagtgttc ggacccattc ttcacgtgct gcgttatcgc tcccgcgatc
     2761 tcgaggacac gatgcgtgcc gtcaacgcga ccgggtacgg actcaccttc ggcgtgcaca
     2821 gccgcatcga cggcgaggcc gaacggctgg ttcgcgaggc cgccgcgggc aattgctacg
     2881 tcaaccgcaa catggtcggc gcggtcgtcg gagtccagcc tttcggcggc tgcggacttt
     2941 ccggtaccgg cccgaaggcg ggcggcccgc attacctgcc gcgattcgcg agcgaaaaat
     3001 ccgtcaccgt caacaccgcg gcggttggcg gcaatgcgag cctcctcgcg atggaggaat
     3061 agaatcctgc ggccggcagc cgtcgcgcgc agcacttcct cgcgtttcat gcagacctcc
     3121 gacttcgatt acgaattgcc ggcgtgcctg atcgcccagc acccgtcgaa cgaacgttcg
     3181 gcgagccgcc tgctggtgct cgacggcgcg agcggtgcga tcgaggaccg ccggatgcgc
     3241 gaacttcccg atctgctcgc agccggcgat cttctggtcg tgaacgacac ccgcgtgatt
     3301 ccggcgcgtc tcctggcaag caagccaagc ggagggcggg tggaactgct gatcgagcgg
     3361 ctcaccggcg a
//
LOCUS       DASUJG010000330         3031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_93619, whole genome shotgun sequence.
ACCESSION   DASUJG010000330 DASUJG010000000
VERSION     DASUJG010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93619"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1250)
                     /locus_tag="VFX38_07075"
     CDS             complement(<1..1250)
                     /locus_tag="VFX38_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX5314648.1"
                     /translation="MAEEIVAPGGDLKAPYRHPLDWKNPEFWDEKALYAEMERQFDIC
                     HTCRRCFPLCHSFPTLFDLIDNSETLELDSVPREAYWKVVDQCYLCDLCFMTKCPYTP
                     PHEFDMDFPHLMLRAKAVRTKNRGAKLRDKVLTSTDAVGRIAGVPVVSQTVNLANKTS
                     PARFALEKTLGVDRRAKLPEYDARSARRRLAKHQPLAGEAQTAGKTCGRVAIYTTCYC
                     EHSAPALIEDLVVVFEHNGIPTRLVTEERCCGMPKMELGDLAAIEKLKNYNVPRLAKI
                     ANEGWDLLAPIPSCVLMYKQELPMIYPGDDSVARVKEAFFDPFEYLMLRHGAGLLNTD
                     FRSPLGKVVCHASCHQRVQNIGAKTRDLLALVPGTTVELIQRCSGHDGTYAVKRETYD
                     NAMKIGRPVFRRIVEAAPDYYGSD"
     gene            complement(1362..1781)
                     /locus_tag="VFX38_07080"
     CDS             complement(1362..1781)
                     /locus_tag="VFX38_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013345268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEX5314649.1"
                     /translation="MELKGSKTHENLKAAFAGESQANRRYLYFAAKADVEGYNDVSAV
                     FRSTAEGETGHAHGHLEYLEAVGDPATGEPIGGTDANLKSAIAGETYEYTDMYPGMAK
                     SARDEGFEEIADWFETLAKAERSHANRFKKALDSLAA"
     gene            2024..2278
                     /locus_tag="VFX38_07085"
     CDS             2024..2278
                     /locus_tag="VFX38_07085"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX5314650.1"
                     /translation="MPESEKETSESPAEDFEAALAALEANVRALEQGDLPLEESLKRF
                     EEGMRLLRRCEAALKQAEQRIEILLAENPDARPEPFDPDA"
     gene            2244..>3031
                     /locus_tag="VFX38_07090"
     CDS             2244..>3031
                     /locus_tag="VFX38_07090"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEX5314651.1"
                     /translation="MPGPSLSIPTPEATTAFGARISAYRERVEAALAHWLPEESESPA
                     RLHAAMRYAALAPGKRVRPLLVYAAGETLGVPVSRLDGPACAVELIHAYSLVHDDLPA
                     MDDDDLRRGRLTCHQAYDEATAILVGDALQTLAFEVLAKDQTMIGDAARRLRMCAALS
                     QAAGSRGMCGGQMVDIEVQGKQIEPSMLDHMHLMKTGALIRVSILLGALSHNEVTEES
                     LADLERFGSRIGLAFQIQDDILDATGDVARLGKTAGADLARAKPT"
BASE COUNT          517 a         1005 c          960 g          549 t
ORIGIN      
        1 cagtcgcttc catagtaatc cggcgcagcc tcgacgatgc ggcggaacac cgggcggccg
       61 atcttcatcg cattgtcgta ggtttcgcgc ttcaccgcgt aggtgccgtc atgaccggag
      121 cagcgttgaa tcagctcgac cgtcgttccg ggaacgagcg cgagcaggtc acgggttttc
      181 gcgccgatgt tctgcacccg ctgatgacag gaggcgtggc agacgacctt gcccagcggg
      241 cttctgaagt cggtgttcag cagtcccgcc ccgtgacgga gcatcagata ctcgaaggga
      301 tcgaaaaacg cttccttcac tctcgcgacc gaatcgtcgc ccggatagat catcggcagt
      361 tcctgcttgt acatgagcac gcaggagggg atgggcgcga gcagatccca gccctcgttg
      421 gcgatcttcg cgagtcgcgg aacgttgtag ttcttcagct tctctatggc cgcaaggtcg
      481 ccgagttcca tcttcggcat gccgcagcag cgttcttccg tcaccagccg ggtgggaatc
      541 ccgttgtgct cgaatacgac gacgaggtct tcgatcagcg cgggcgcgct gtgctcgcag
      601 tagcaggtgg tgtagatcgc gacccgcccg caggttttcc ccgcggtctg cgcttcaccc
      661 gcgagcggtt ggtgtttcgc gagacgccgc cgcgcgctcc tggcatcgta ctcgggcagc
      721 ttcgcccggc ggtccacgcc cagcgttttt tcgagcgcga accgggccgg cgaagtcttg
      781 ttggcgaggt tcacggtctg ggagacgacc ggaacgcccg cgatcctgcc gacggcatcg
      841 gtggacgtga gcaccttgtc gcgcagcttc gcaccccgat tcttcgtgcg caccgccttc
      901 gcccgcagca tcagatgcgg aaagtccatg tcgaactcgt gcggcggcgt gtacgggcac
      961 ttcgtcatga agcagagatc gcagagataa cactgatcca cgaccttcca gtacgcctcg
     1021 cgcggcaccg aatcgagttc gagcgtctcg gaattgtcga tcagatcgaa aagcgtcggg
     1081 aacgaatggc agagcggaaa gcagcgccgg caggtatgac agatgtcgaa ctgccgttcc
     1141 atctcggcgt acagcgcctt ctcgtcccag aattcgggat tcttccagtc gagaggatgg
     1201 cggtacggag ccttgaggtc tcctccgggc gcaacgatct cttcggccat gtctcctgct
     1261 ccccgcaaat cggaccacgg atgtgccgcg caatgccggc aacggacctg cgaccttatg
     1321 ccggcctgcg tcgactccga ttggcgatcg gagattccgg gctacgccgc aagggaatcg
     1381 agcgccttct tgaaacggtt ggcgtgcgag cgctccgcct tcgcgagcgt ctcgaaccag
     1441 tcggcgattt cctcgaaacc ttcgtcgcgc gcggacttcg ccatgcccgg atacatgtcg
     1501 gtgtattcgt aggtctcacc cgcgatcgcc gacttcagat tcgcgtcggt cccgccgatc
     1561 ggctcgccgg tcgccggatc gcccaccgcc tcgaggtatt cgagatgccc gtgtgcgtgg
     1621 cccgtttcgc cttccgccgt cgagcggaat acggcggaaa catcgttgta gccttcgacg
     1681 tccgccttgg ccgcgaaata cagatagcgc cgattcgcct gcgattcgcc ggcgaaggcg
     1741 gccttgagat tctcgtgtgt cttgctgccc ttgagttcca ttgctctatc tcctgattgg
     1801 caaaggtccc gcggggaagg aaacggctcc ccgcaagcgt tgcggcggca cgcccgggcg
     1861 ccgcccagcg aatttagact gggtctaaat tcgggcccag tgtaggcagc ggggccggca
     1921 ctgtcaatcc gggcccgtat tgacgtgcgg ggccccggcg tgtacggttc gccttcagga
     1981 tcggcgcgcc cgccggtcac cgaatccgac cagcgagaat cgcgtgcccg aatccgaaaa
     2041 ggaaaccagc gagagtccgg ccgaggactt cgaggccgcc cttgcggcac tggaagccaa
     2101 cgtgcgcgcc ctcgaacagg gcgatctgcc gctggaggaa tcgctcaagc gcttcgagga
     2161 aggcatgcgt ctgctccgcc gctgcgaggc cgcgctcaag caggccgaac agcggatcga
     2221 aatccttctc gccgagaatc ccgatgccag gcccgagcct ttcgatcccg acgcctgagg
     2281 cgacgaccgc cttcggcgct cgcatctccg cctaccgtga gcgggtcgag gccgcactcg
     2341 cgcactggtt gccggaagaa tcggagagcc ccgcgcgact ccatgccgcg atgcgctacg
     2401 ccgcgctcgc gccgggcaag cgcgtgcgcc cgctgctcgt ctacgccgcc ggggaaacgc
     2461 tcggggtgcc ggtctcgcgc ctcgacggcc ccgcctgcgc ggtggagttg atccacgcct
     2521 actcgctcgt ccacgacgac cttccggcga tggacgacga cgatctgcgc cgcgggcgcc
     2581 tcacctgcca tcaggcctat gacgaggcga ccgccattct cgtcggcgat gccctgcaga
     2641 cgctcgcctt cgaagtgctc gcgaaggacc agacgatgat cggcgatgcc gccaggcgtc
     2701 tgcgcatgtg cgcggcgttg tcgcaggcgg cgggctcccg cggcatgtgc ggcgggcaga
     2761 tggtggacat cgaggtacag ggcaagcaga tcgagccgag catgctcgat cacatgcatc
     2821 tcatgaaaac cggcgcgctc atccgcgtga gcattcttct cggcgcgctc agccacaacg
     2881 aagtcaccga agaatcgctc gcggatctcg aacgcttcgg ctcgcgcatc gggctcgcct
     2941 tccagattca ggacgacatc ctcgacgcca cgggcgacgt cgcgcgcctc ggaaaaaccg
     3001 cgggcgccga tcttgcacgc gcgaaaccga c
//
LOCUS       DASUJG010000331         4168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_339228, whole genome shotgun sequence.
ACCESSION   DASUJG010000331 DASUJG010000000
VERSION     DASUJG010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339228"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /locus_tag="VFX38_07095"
     CDS             complement(<1..1172)
                     /locus_tag="VFX38_07095"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEX5314652.1"
                     /translation="MLSSFRKHTKGWIAWVFVILISVPFALWGIGQYRSVFTTDYIAK
                     VNGEKIMPATFQRSWQQFYQQQENTLGDKFNPSDKEQTALKQQVLNQLIDQTLMRQQA
                     AKNRFVASEADLQAQIEQIPAFQTGGHFDFAQYQTVLAANGYTTKQFESSMRTDLASQ
                     ELQNGLAQSAFAVPKESNALLDLLQQQRRIAWFTLPLDQFRKDQKAPDDKDIQAYYDA
                     HREQFVVPTTLTIAYVRLGPKQLEARVKPTPEELKAYYASHQNQYGIPPARKAAEIVI
                     APTPAGTKGWAAAKTQATKLLGQIRAAKDGQKEFAALAAKYSDDATSKRNGGSLGWIG
                     SGQMPTVFDQALFGLKTTGDVAGPIRTAKGWSLIQLLGTRGGSVKPYDEVSAKVEK"
     gene            complement(1339..1415)
                     /locus_tag="VFX38_07100"
     tRNA            complement(1339..1415)
                     /locus_tag="VFX38_07100"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1379..1381),aa:Asp,seq:gtc)
     gene            complement(1422..1496)
                     /locus_tag="VFX38_07105"
     tRNA            complement(1422..1496)
                     /locus_tag="VFX38_07105"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1462..1464),aa:Val,seq:tac)
     gene            complement(1510..1782)
                     /locus_tag="VFX38_07110"
     CDS             complement(1510..1782)
                     /locus_tag="VFX38_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX5314653.1"
                     /translation="MNKSELIEAVASETGLSKAEAGRASDAVFNCITTALKRGDQVTL
                     VGFGSFMVRERKARTGRNPQTGGTMNIPASKTPGFRAGKALKDAVN"
     gene            complement(1972..>4168)
                     /gene="lon"
                     /locus_tag="VFX38_07115"
     CDS             complement(1972..>4168)
                     /gene="lon"
                     /locus_tag="VFX38_07115"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005004534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX5314654.1"
                     /translation="VGTLATILQLLKLPDGTVKVLVEGQERLVVRALVAGDSFSAHGE
                     TIAEAEEPANRETEALLRTALSLFEQYVKLSRKIPAELLGALGSVGEPGRLADTMAAH
                     LSLRLEDRQALLEQFDPRARLEQVMELITGETDLLQIERRIRGRVKQQMEKSQREYYL
                     NEQMKAIQKEMGELEEGGGELDDLQRRIARSGMPREARERAESELGKLRQMPPMSAEA
                     TVVRNYLDWILMVPWKKRTRLRHDLVAAQAVLEADHYGLEKVKERVIEFLAVQQRVRR
                     LKGPILCLVGPPGVGKTSLGQSIARATNRRFARASLGGVRDEAEIRGHRRTYIGSMPG
                     KIMQKLARVGTRNPLFLLDEIDKMSVDFRGDPSAALLEVLDPEQNHVFSDHYLEVDFD
                     LSEVLFIATANTLNIPAPLADRMEIIRIPGYTEDEKKAIARRYLLPKQIKANGLKKRE
                     LEVSDGAVESMIRLYTREAGVRSLEREIAKLCRKTVTGLLLDPEREVPVRVDSGDLSS
                     LLGVERYRYGHADEDDQIGQVVGLAWTEVGGELLRIEAATVPGKGRLTHTGQLGDVMK
                     ESVQAALTVVRSRARSLGIADAAFQERDFHVHVPEGATPKDGPSAGIGMATALVSALS
                     GIAVRADVAMTGEITLRGEILPIGGLKEKLLAAQRGGLKTVLIPEENVKDLAEIPDNV
                     KSGLELKPVRWIDTVLSQALVREPRPLEETPLVPGTTTAGETVRGRPAH"
BASE COUNT          655 a         1352 c         1334 g          827 t
ORIGIN      
        1 tccttttcga ctttcgcgct cacctcgtcg tagggcttga ccgagccgcc gcgggtgccg
       61 agcagttgga tcaggctcca gcctttcgcg gtgcggatcg gcccggccac atctccggtg
      121 gtcttgagtc cgaaaagcgc ctgatcgaag accgtcggca tttgcccgct tccgatccag
      181 ccgagcgagc cgccgttgcg tttggaggtg gcatcgtcgg aatacttggc ggcgagcgcg
      241 gcgaattcct tctgcccgtc cttcgcggcg cgaatctgcc cgagaagttt cgtcgcctgc
      301 gtcttggccg cggcccagcc cttggtgccc gctggcgtcg gcgcaatgac gatctcggcc
      361 gccttgcgcg cgggcggaat gccgtactga ttttgatgcg aggcgtagta cgccttgagt
      421 tcttcggggg tcggcttgac gcgcgcttcg agttgcttcg gaccgagacg aacgtaggcg
      481 atggtaagcg tcgtcggaac gacaaactgc tcgcggtgcg cgtcgtagta ggcctgaatg
      541 tccttgtcgt ccggcgcctt ctggtccttg cggaactgat cgagcggcag ggtgaaccac
      601 gcgatccggc gctgctgctg cagcagatcg agcagcgcat tcgactcctt cggcaccgcg
      661 aaggcgctct gcgcgaggcc gttctggagt tcctgcgagg cgagatcggt gcgcatgctg
      721 ctttcgaatt gcttggtcgt gtagccattg gcggcgagca cggtttgata ctgcgcgaag
      781 tcgaaatgcc cgccggtctg gaaggccgga atctgctcga tctgcgcctg cagatcggcc
      841 tcgctcgcga cgaagcggtt cttcgccgcc tgctggcgca tcagggtctg atcgatgagt
      901 tgattcagaa cctgctgctt gagcgccgtc tgctccttgt cggagggatt gaatttgtcg
      961 ccgagggtat tttcctgctg ctggtagaac tgctgccagg agcgttggaa ggtcgccggc
     1021 atgatcttct cgccgttgac cttggcgatg tagtccgtag tgaagacact gcgatactgg
     1081 ccgatgcccc agagcgcaaa gggaacgctg atcaggatca cgaagaccca ggcaatccag
     1141 cccttggtgt gttttcgaaa actcgaaagc atccttcatc cttccgtgtc gcggctcgat
     1201 gtgccgcgga aaagctcgcc ggggaatggc gcgcgggcaa ggcgtcgccg cgcacttttt
     1261 cgcgcccttg ccatgcgcaa acccgcgacg atccggcccg aaaaggcgag agggtgccgt
     1321 tgcgcaccct ctcgcggatg gcggagtgga cgggactcga acccgcgacc cccggcgtga
     1381 caggccggta ttctaaccag ctgaactacc actccgtggc atggtgggtg ctgagggatt
     1441 tgaacccccg actttcgcct tgtaagggcg acgctctacc cctgagctaa gcaccccggg
     1501 ggcagcggat tagtttacgg catccttgag ggccttgcca gcgcgaaagc ccggcgtctt
     1561 gctcgccgga atgttcatcg tgccgcccgt ctgcgggttg cggccggtac gcgccttgcg
     1621 ctcgcgcacc atgaaactgc cgaaaccgac gagggtgacc tgatctcccc gcttgagcgc
     1681 ggtggtgatg caattgaaaa ccgcgtccga tgcgcgtccc gcttccgcct tcgacaggcc
     1741 ggtttccgag gcaacggcct cgatcagttc tgacttgttc atgggaaaac gtaccccttg
     1801 gtgcaaatgt ggtgagtgaa tgcgatgtcc gcctcgcccg ttcgaccgcg atccgggcga
     1861 cccgcgagct tcgccgcggc ctctgcttta taccagcgca gtcaaaactc tgtcaaaccc
     1921 cggcgcggcg gcggtcgagt cttgccgcgc tttcgcaggc gcgggtaacc gtcaatgggc
     1981 cggtcggccg cgaaccgtct cgcccgccgt cgttgtaccc ggaacaagcg gggtctcctc
     2041 cagcgggcgc ggctcgcgca ccagcgcctg actgagcacc gtatcgatcc agcgcaccgg
     2101 tttcaattcc agcccgctct tcacgttatc gggaatttcc gcgagatcct tgacgttttc
     2161 ctcgggaatg agcacggttt tcaagccgcc gcgctgggcc gccagaagct tttccttgag
     2221 accgccgatg ggaagaatct ctccccgcaa ggtgatctcg ccggtcatcg cgacgtcggc
     2281 gcgcaccgcg atgccgctca gcgcggaaac cagcgcggtc gccatgccga ttccggcgct
     2341 cggtccgtcc ttcggcgtcg cgccctccgg cacatggacg tgaaaatcgc gctcctgaaa
     2401 cgcggcgtcc gcgatcccga gcgaacgcgc gcggctgcga acgacggtga gggccgcctg
     2461 cacggattcc ttcatgacgt cgccgagttg tcccgtatgc gtgaggcgcc ccttgccggg
     2521 caccgtcgcg gcctcgatgc gcagcagctc cccgcccacc tcggtccagg cgagtcccac
     2581 cacctgcccg atctggtcgt cttcgtcggc gtgcccgtaa cgatagcgct cgactccgag
     2641 aagcgaggaa agatcgccgc tgtccacgcg caccggcacc tcgcgctcgg gatcgagaag
     2701 caatccggtc acggtcttgc ggcagagttt ggcgatttcc cgctcgagac tgcgcacgcc
     2761 cgcctcgcgc gtatagaggc ggatcatgga ttcgacggcg ccatcgctca cctcaagttc
     2821 ccgcttcttg aggccgttgg ccttgatctg cttcgggagc agatagcgcc gggcgatcgc
     2881 cttcttttcg tcttccgtgt agccggggat gcggataatc tccatgcggt cggcgagcgg
     2941 cgcgggaatg ttgagcgtgt tcgcggtcgc gatgaagaga acttccgaga gatcgaaatc
     3001 cacttcgaga tagtgatcgc tgaagacatg attctgctcg ggatcgagta cctcgagcag
     3061 tgcggccgag ggatcgccgc ggaaatccac cgacatcttg tcgatctcgt cgagcagaaa
     3121 aagcgggttg cgcgttccca cgcgcgcgag cttctgcatg atcttgccgg gcatcgaccc
     3181 gatgtaggtg cggcggtggc cgcggatctc ggcttcgtcg cgcacgccgc cgagcgaagc
     3241 gcgcgcgaag cgtcggttgg tggcccgcgc gatgctctgc ccgagcgaag tcttgcccac
     3301 gccgggcggg ccgacgaggc agagaatcgg gcccttgaga cggcgcaccc gctgctgcac
     3361 cgcgagaaac tcgatcaccc gctccttcac tttctcgagg ccgtagtgat cggcttcgag
     3421 caccgcctgc gccgcgacga gatcgtgacg caggcgcgtg cgcttcttcc acgggaccat
     3481 caggatccag tcgagataat tgcgcacgac cgtcgcctcg gccgacatcg gcggcatctg
     3541 acggagcttg ccgagttcgg attcggcgcg ttcgcgcgct tcccggggca tccccgagcg
     3601 ggcaatgcgc cgctgcagat cgtcgagttc gccgccgcct tcctcgagct cgcccatttc
     3661 cttctggatg gccttcattt gctcgttgag gtagtactcg cgctggctct tttccatctg
     3721 ctgcttgacg cggccgcgaa tccgtcgctc gatttgcagc agatccgtct cgccggtgat
     3781 gagttccatc acctgttcga gtcgcgcccg cggatcgaac tgttcgagaa gagcctggcg
     3841 gtcttcgagg cgcaggctca ggtgcgcggc catggtgtcg gcgagccttc ccggctcgcc
     3901 caccgagccg agcgcgccga gaagttcggc cggaatcttg cggctcaact tcacgtattg
     3961 ctcgaagagc gacagcgccg tgcgcagcag cgcctcggtt tcccggttcg ccggctcctc
     4021 cgcttcggcg atcgtctcgc cgtgcgcgct gaagctgtca ccggcgacga gcgcccgtac
     4081 cacgagacgc tcctgacctt cgaccagcac cttcaccgtg ccgtcgggca gcttcaggag
     4141 ttgcaggatg gtcgcgaggg tgccgacg
//
LOCUS       DASUJG010000332         2160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1684216, whole genome shotgun sequence.
ACCESSION   DASUJG010000332 DASUJG010000000
VERSION     DASUJG010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1684216"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VFX38_07120"
     CDS             complement(<1..451)
                     /locus_tag="VFX38_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314655.1"
                     /translation="MGLFSPATVWCFAFAGYLLFAGTVSRHELITSAALASGAVAWAY
                     AILRCSSRRYAMTPAHAVAWLKAIRGLVPAIARTLIVLLKAAVLGGSPGRALELAFLR
                     GPEDEPEDRARRAAAVLIASLAPDTYVVRAPHQRDCVLVHTVLRSNTP"
     gene            complement(541..1395)
                     /locus_tag="VFX38_07125"
     CDS             complement(541..1395)
                     /locus_tag="VFX38_07125"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5314656.1"
                     /translation="MAGESEFVAFWNKVLVPKFLRFQDILVEGFGKHSRIVFERGAIA
                     SPGERVLDVGCGFGDTAIDLARQTGANGQVVGIDCCDTFLDIGRRDAGNARVENIEWL
                     EGDAETENFDAPFDLWFSRFGTNFFSTPVAALRHLREQLKPDGRAMMIVWQARDRNRW
                     AALPREVVLRFLPEPEAPEHCGPGPFSMGDRELVTRQLQAAGYSDIAFTPVQADIMVG
                     RTVDDAIDFQLALGPSGEIWREAGELAERRAEEIRAALHETLKPYLTDEGVLLPSASW
                     CITARNPG"
     gene            complement(1540..>2160)
                     /locus_tag="VFX38_07130"
     CDS             complement(1540..>2160)
                     /locus_tag="VFX38_07130"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX5314657.1"
                     /translation="DVWIQRGVAGNLDVQAKGIQQHLANKAIDLYTFASRPQLGLVAS
                     YSDTRQSGDLSFIVAPDRRQNAAIGLQLSIPIFSGGGLVSQLRQARATSREATYELQA
                     SQADVTVKVREVFQSIQAGVKGIQALEQAAVTAKTSLEANQLALKVGVKSTQDVLDAE
                     QTYYQTLEQLEQARYQYLMGMLTLQLLAGNLDEAAVARINREQHGP"
BASE COUNT          384 a          693 c          678 g          405 t
ORIGIN      
        1 gcggcgtgtt cgaccgcaga accgtatgaa ccagcacgca atctcgttga tgaggggccc
       61 gcactacgta agtgtccggg gccagcgatg cgatcagtac cgctgccgca cgccgggcgc
      121 gatcctctgg ttcgtcttcc ggcccacgca agaaagcgag ttcgagagcc cgccccggag
      181 agccgccgag cacggcggcc ttcaacagta cgatcaaggt cctggcgatg gcgggaacaa
      241 ggccgcggat tgccttcaac caggcgactg catgggcggg ggtcatcgcg tagcgccgcg
      301 acgaacagcg caaaatcgca taggcccacg ccaccgcgcc ggatgcgagc gccgccgacg
      361 tgatcagttc gtgacgactc accgtgccgg cgaagagcag atagcccgcg aaggcaaagc
      421 accacaccgt cgctggggag aacaatccca tcccgcacgc tccttgcccg gccaacaccc
      481 tgcaggcatt ctatcgcctg gacgatgctt ccgcgggaat gacggccaag ggcagcgctg
      541 tcagccggga tttcgtgcgg ttatgcacca cgacgcggac ggaagcaaga cgccctcgtc
      601 ggtcaagtac ggcttcagcg tttcgtggag cgccgcgcgg atttcttcgg cccggcgttc
      661 ggcgagttcg cccgcctcgc gccagatttc tcccgagggg ccgagcgcga gctggaaatc
      721 gatcgcgtcg tccaccgtgc gccccaccat gatgtcggct tgcacaggcg tgaaggcgat
      781 atccgagtag cccgcagcct gcaactgccg ggtgacgagt tcgcggtctc ccatcgagaa
      841 gggtccgggt ccgcaatgtt ccggagcttc cggctccggc aggaatcgca gcacgacctc
      901 gcgcggcagc gccgcccagc gattgcggtc acgcgcctgc cacacgatca tcatcgcgcg
      961 tccgtccggt ttcaactgct cgcgcaagtg acgaagcgcc gcgacgggcg tggaaaaaaa
     1021 gttggtgccg aagcgcgaaa accacaagtc gaagggcgcg tcgaagttct cggtttcggc
     1081 atcgccttcc agccattcga tgttttcgac tctcgcgttc ccggcatcac gacggccgat
     1141 atcgaggaag gtgtcacagc aatcgattcc gaccacctgc ccgtttgcgc cggtctggcg
     1201 cgcgaggtcg atcgcggtat cgccgaagcc gcagccgacg tcgagcacgc gctcgcccgg
     1261 actcgcgatg gcgccgcgct cgaagacgat gcggctgtgc ttgccgaagc cttcgaccag
     1321 gatgtcctgg aagcgcagga atttcggcac gagcaccttg ttccagaacg cgacaaattc
     1381 ggattcgccg gccatggtct gttccttgcc ttgtcgcgcg aattatagac ctgcgccccg
     1441 cgctgccggt tcacgtcccg aatcgcctgg gaatggactg tttctgtctt tgtatcaata
     1501 acttacattt tgttatacaa gctgtttccg gaaaaaattt cagggtccgt gctgttcccg
     1561 gttgattcgg gcgacggcgg cctcatccag attgccggcg aggagttgca aggtgagcat
     1621 gcccatgagg tactgatagc gtgcctgctc gagttgttcg agcgtctggt agtacgtctg
     1681 ctcggcgtcg agcacgtcct gcgtggattt cacgccgacc ttgagcgcga gctggttcgc
     1741 ttcgagcgag gtcttcgcgg tgactgccgc ctgttccagt gcctgaattc ccttgacgcc
     1801 cgcctgaatg ctctggaaaa cctcccgcac tttgacggtc acgtcggcct gcgacgcctg
     1861 cagttcgtag gtcgcctcgc ggctggtcgc ccgcgcttga cgcaactgcg acacgagccc
     1921 gccgccgctg aagatcggaa tgctgagctg gagcccgatc gccgcattct ggcgccggtc
     1981 cggtgcgacg atgaacgaca ggtctccgct ctggcgcgta tccgaatagc tcgcgacgag
     2041 tccgagttgc gggcggctcg cgaaggtgta gagatcgatt gccttgttgg cgagatgctg
     2101 ctggatcccc tttgcctgca cgtcgagatt gccggcgacg cctcgctgaa tccagacgtc
//
LOCUS       DASUJG010000333         6195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1562665, whole genome shotgun sequence.
ACCESSION   DASUJG010000333 DASUJG010000000
VERSION     DASUJG010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6195
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1562665"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /gene="bioD"
                     /locus_tag="VFX38_07135"
     CDS             complement(<1..418)
                     /gene="bioD"
                     /locus_tag="VFX38_07135"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEX5314658.1"
                     /translation="MKGYFVTGTDTDAGKTVVAAGMIHAFAARGLRVAAMKPVASGGH
                     EQATGLRNADAEALIAAANVAAEYEEVNPYVFAPPIAPHIAAKETGVAIAMETILRAA
                     RALAARADLLLVEGVGGWRVPLGEDFDVAALAAALDL"
     gene            complement(415..1227)
                     /gene="bioC"
                     /locus_tag="VFX38_07140"
     CDS             complement(415..1227)
                     /gene="bioC"
                     /locus_tag="VFX38_07140"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017942923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102130 - malonyl-CoA methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HEX5314659.1"
                     /translation="MSDAANRDARRAAFERAAAGYERAAVLQAEVQERLLERLELTTI
                     APRRILDLGAGTGRALGPLARHYPGAAVFAADFAVAMLARARRRGRWFRHFPAAAARA
                     ETLPFARGSFDLVFSNLMLQWCPDLENVFSEARRILSDRALLLFATLGPDTLRELRDA
                     WAEVDEHAHVNRFIDMHDLGDALIRAGFVEPVMDVEEITLTYDQVHDLLRDLKAIGAH
                     NVHADRATGLGGPRRFAAFERAYERFRSDGRLPATYEVVYGTAWTPIDLPRR"
     gene            complement(1220..1972)
                     /locus_tag="VFX38_07145"
     CDS             complement(1220..1972)
                     /locus_tag="VFX38_07145"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5314660.1"
                     /translation="MPEPLPVFALHGWALNAAVFAPLRAWCAGRWLVTPDLPGHGQRH
                     DESLGRDPDAIVQRLLAEAPPRSLWLGWSLGSLLTLAAALRQPERMTALVLVASTPCF
                     VAREDWPAGMARENLERLAVSLEHDPAAAVNDFLTWQVRSNERGIAALSGLRGALAER
                     GNASPEALADGLALLEAMDYRAELARLTMPVLVIAGGRDRLVRPAAARTLAAQLPQAR
                     LAMIAQAAHAPFLSHPLEFRAALHEFLATLDE"
     gene            complement(1965..3131)
                     /gene="bioF"
                     /locus_tag="VFX38_07150"
     CDS             complement(1965..3131)
                     /gene="bioF"
                     /locus_tag="VFX38_07150"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEX5314661.1"
                     /translation="MNLLDERLRRDLQALEGRALRRRLAVHDETAFPQITFAGRRMLD
                     FAANDYLGLAQHPRVSAALARGAERWGAGAGAAHLLGGHSRAHADFEEALADFLGREA
                     ALLFSTGYMANLGLVSTLVGRGDTVIADRLDHASILDAATLSRARLLRYPHGDCAAAA
                     TRTRGARGRRLIVTDGVFSMDGDLAPLPALAALAEESDAIFAVDDAHGFGVLGAGGRG
                     SLEHFGLDAQAVPALVVTLGKALGVFGAAVVGSRALIETLVNRARPWIYTTALPPALA
                     EALCVALEVLREEAWRRERVAALVARFRASAAAAGLPLLASSTPIQPILVGDAARALG
                     FSKALADRGYFVPAIRPPTVPAGAARLRISLTAAHSESDLDGLVAALVGMFADA"
     gene            complement(3158..4114)
                     /gene="bioB"
                     /locus_tag="VFX38_07155"
                     /pseudo
     CDS             complement(3158..4114)
                     /gene="bioB"
                     /locus_tag="VFX38_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005894359.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
     gene            complement(4177..4566)
                     /locus_tag="VFX38_07160"
     CDS             complement(4177..4566)
                     /locus_tag="VFX38_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014995843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HEX5314662.1"
                     /translation="MTAPRPSQRPYFLRAYHAWVTDSGLTPHLIVNAAAPGVEAPAAQ
                     AQDGRLVLNVSLQATDELTLGNEAVEFSARFGTAVHHVRIPIGAVLGLYARETGEGLA
                     FAPDESPGDAPAGPGERPERPRLTIVK"
     gene            complement(4563..5165)
                     /locus_tag="VFX38_07165"
     CDS             complement(4563..5165)
                     /locus_tag="VFX38_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5314663.1"
                     /translation="MILYSHPDDPAGHAMRLILAEKGVAAKLVEVSGQAPPDDLLSLS
                     PYGNMPTLVTREVTLYDPRVIAEYLDDRYPHPPLMATEPALRARIRLFIGEVGGEWYG
                     QCRQIAKGTSREKTAARHALGETLIANDEVFAVAPYLLSESYGLADCIVAPVLWRLPA
                     LGIPLPREARALRGYMQRVFRRPSFVVSLTGSERAMATAV"
     gene            complement(5203..>6195)
                     /gene="murA"
                     /locus_tag="VFX38_07170"
     CDS             complement(5203..>6195)
                     /gene="murA"
                     /locus_tag="VFX38_07170"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019642635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX5314664.1"
                     /translation="RTMRASILVLGPMLARYGRADVSLPGGCAIGSRPVDLHLKGLRA
                     MGAKIRVAGGYIQAHAKRLKGARILMIPVSVTGTENLMMAATLAAGETVIENAAREPE
                     VVDLAQCLIQMGANIAGAGTDVIRIQGVESLHGTRYEVLPDRIETGTYLVAGALTGGH
                     VKVKRTRPAMVESILLKLTETGARITSDGDWIELDMEGRRPQAVSIRTAPYPEFPTDM
                     QAQFMALNAVAEGSGTITETVFENRFMHVQELVRMGAAIQLEGNTAFTNGVTNLKGAP
                     VMATDLRASASLVLAGLVAEGETVVDRIYHIDRGYECIEEKLAQLGAHIRRIPA"
BASE COUNT         1051 a         2073 c         2133 g          938 t
ORIGIN      
        1 gaagatcaag cgcggcggca agcgcggcga cgtcgaaatc ctccccgagc ggcacgcgcc
       61 agccgccgac gccctcgacg aggaggagat cggcgcgggc cgcgagagcg cgcgcggcgc
      121 ggagaatcgt ttccatcgcg atggcgacgc cggtctcctt ggcggcgatg tgcggcgcga
      181 tcggcggcgc gaaaacgtag ggattcacct cctcatattc ggccgcgaca ttcgccgccg
      241 cgatcaatgc ctcggcatcg gcgttgcgca ggcccgtcgc ctgctcgtgt ccgccgctcg
      301 ccaccggctt catcgccgcc actcgcagtc cgcgagccgc gaatgcatga atcatgcccg
      361 ccgcaaccac ggtcttgccg gcgtcggtat cggttccggt aacgaagtac cctttcatcg
      421 gcgcggcaga tcgatcggtg tccaggcggt accgtacacg acttcgtagg tcgccggaag
      481 tcgcccgtcg ctgcgaaaac gctcgtaggc acgttcgaag gcagcgaaac gccgcggtcc
      541 gccgaggccc gtggcgcgat cggcgtgcac gttgtgcgca ccgattgcct tgaggtcgcg
      601 gaggagatcg tgcacctgat cataggtgag cgtgatttct tcgacatcca tcaccggctc
      661 gacgaaaccc gcgcgaatga gggcatcacc gagatcgtgc atgtcgatga agcgattcac
      721 gtgtgcatgt tcgtccactt ccgcccaggc gtcgcgcagt tcccgcagag tgtcgggacc
      781 gagcgtcgca aagagaagaa gcgcccgatc cgagagaatc cgccgcgcct cggagaaaac
      841 attctcaaga tcgggacacc attgcaacat caggttgctg aaaacgagat cgaaactgcc
      901 gcgcgcgaaa ggcaaggtct cggcgcgcgc agccgcggcg ggaaagtgcc ggaaccagcg
      961 cccgcgtcgt cgcgcgcgcg cgagcatcgc gaccgcaaag tccgcggcga acacggcggc
     1021 gcccggatag tggcgggcga gcggcccgag agcacggccg gtgccggcgc ccagatcgag
     1081 gatccgccgg ggcgcgatcg tggtgagttc gagacgctcg agcagccgct cttgcacctc
     1141 ggcctgcagt acggccgcgc gctcgtagcc ggcggccgcg cgctcgaagg ccgcgcggcg
     1201 ggcatcgcga ttggccgcgt cactcatcga gggtcgcgag aaactcgtgc aacgcggctc
     1261 gaaactcgag cggatgggaa agaaagggcg cgtgcgccgc ctgcgcgatc atcgcgaggc
     1321 gcgcttgcgg cagttgcgcc gcgagggttc gcgccgctgc cgggcgcacc agccgatcgc
     1381 ggccgccggc gatcaccagc accggcatcg tcaggcgcgc gagctcggct cggtagtcca
     1441 tcgcttccag cagggcgagt ccgtcggcga gcgcctcggg gcttgcgttg ccgcgctcgg
     1501 caagggcgcc gcgcagcccc gaaagggccg cgattccacg ttcgttcgag cgcacctgcc
     1561 aggtcagaaa atcgttcacc gccgccgccg gatcgtgctc cagactcacg gcgaggcgct
     1621 ccagattctc ccgcgccatg cccgccggcc agtcctcgcg cgcgacgaag cagggcgtgg
     1681 aggcaaccag taccaaggcc gtcatgcgct ccggttgccg caacgcggca gcgagagtca
     1741 acaaggaacc gagcgaccag cccagccaca gcgagcgcgg cggcgcctcc gcaagaagac
     1801 gctgcacgat ggcgtcggga tcgcgtccga ggctttcgtc atggcgctga ccgtgacccg
     1861 gcagatcggg ggtgacgagc caacgcccgg cgcaccatgc acgaaggggc gcgaatacgg
     1921 ccgcgttgag cgcccagccg tgcagggcaa agaccggaag cggctcaggc atcggcgaac
     1981 atcccgacaa gagcggcgac gagtccatcg aggtcggatt cgctgtgcgc agccgtgagg
     2041 gaaatgcgca gccgcgcggc tcccgccggc accgtcggcg gccggatcgc cggcacgaag
     2101 tagccgcgat cggcaagggc tttggagaat ccgagggcgc gcgccgcatc gcccaccagg
     2161 attggttgaa tgggcgtcga ggaagcgaga agcggcaggc cggcagccgc agcggaggcg
     2221 cgaaaccgcg cgacgagtgc ggctactcgt tcgcggcgcc aggcctcttc ccgcaggacc
     2281 tcgagcgcga cgcacagcgc ctcggcgagc gcggggggca gcgccgtggt gtagatccag
     2341 ggacgggcgc ggtttacgag ggtctcgatc agggcgcggc tgccgacgac ggcggcgccg
     2401 aataccccga gcgccttgcc gagcgtcacc acgagcgcgg gaacggcttg cgcatcgagt
     2461 ccgaaatgtt cgagactgcc gcgccctccc gcgccgagga cgccgaaccc atgcgcatcg
     2521 tccacggcga aaatcgcatc gctttcctct gcgagcgcgg cgagagccgg aagcggggcg
     2581 agatcgccgt ccatgctgaa gaccccgtcg gtcacgatca ggcgtcgtcc gcgcgcgccg
     2641 cgggttctgg tcgcggccgc cgcacaatcg ccgtgcggat agcggagcag tcgcgctcgc
     2701 gacagtgtcg cggcatcgag aatcgaagcg tgatcgaggc gatcggcgat gaccgtatcg
     2761 ccgcgaccga cgagcgtgga taccagcccg aggttcgcca tgtagcccgt agagaaaagc
     2821 agcgccgcct cacgcccgag aaaatccgcg agcgcctctt cgaagtccgc atgcgcgcgc
     2881 gaatgcccgc ccagaagatg cgccgcgccg gcgcccgctc cccagcgctc ggctccgcgc
     2941 gcgagcgccg ccgagacacg cggatgctgt gcgagtccca gatagtcgtt ggcggcgaag
     3001 tcgagcatcc gccgtcccgc aaacgtgatc tgcggaaagg ccgtctcgtc atgaacggcc
     3061 aggcggcggc gcagagcgcg cccttcgagc gcctggagat cgcgccgcaa gcgctcgtcg
     3121 aggaggttca tcccgccgcg agctgctccg cgggagtttc cgcgcgcagg ccgaggcggt
     3181 caaagagagc gcgatcggca tcgcgctgcg gattgggcgt ggtgagcagg gtctcgccgt
     3241 agaagatcga attcgcaccg gcaaggaagc agagcgcctg caactcgtcg ctcatctgcg
     3301 tgcgcccggc ggcgagacgc acatgcgcga gcggcatcag gatccgcgcc gtcgcgatcg
     3361 tgcgcacgaa ctcgagcgga tcggcgctgg cattgtgcgc gtagggcgtg ccctccactc
     3421 gcaccagttc gttgatggga acgctctcgg gatgctgcgg cagggtggca agttgctgca
     3481 gcaggccgat gcgatcctcc gacgattcgc ccagcccgac gatgccgccg cagcagacct
     3541 tgatgccggc agctcgcact tttgcgagag tatcgaggcg atcctgatag ctgcgcgtcg
     3601 tgatcacctc gccatagaac tcgggcgagg tatcgagatt gtggttgtaa tagtcgagcc
     3661 cggccgcggc cagccgctcg gcctgatcgc ctgcgagcat gccgagggtg agacaggttt
     3721 ccattcccat cgatttgatt tcgccgatca ggggcagcag cggttcgagg tcgcggtcct
     3781 tcggccggcg ccaggcggcg cccatgcaaa agcgcgtcgc cccggcggtc cgcgcggccc
     3841 gcgcacgcgc catcacggtc tcgcggtcga gaagcttttc gttttcgacg ccggtgtgat
     3901 agcgcgcact ctgcggacag tacttgcagt cttcgggaca gccgccggtc ttgatcgaca
     3961 gaagcgtcga gatctgcacc gcgttcggat cgaacacggc acggtgcacg gactgcgcgc
     4021 gaaagagaag atcgttgagc ggcgcgtggt acagcgccgc cacttcctcg cggctccaat
     4081 cggtgcggga gacaacggac ggagtcggcg tcatggacga gctgcgcggg gcaatcgttt
     4141 cagtctagca gacggattac ttcgatccgg cggcaattat ttgacgatcg taagccgcgg
     4201 ccgttccggg cgctcccccg gaccggcggg ggcatcgccg gggctctcgt cgggcgcgaa
     4261 tgcgagcccc tctccggttt cgcgggcgta cagtccaagc acggcgccga taggaatgcg
     4321 aacatgatgg accgcggtgc cgaaacgggc agagaattcg actgcctcgt tgccgagcgt
     4381 cagctcgtcg gtcgcctgca ggctgacgtt gagtaccagc cggccatcct gcgcctgcgc
     4441 ggcgggagcc tccacgccgg gcgcggcggc gttcacgatg agatggggcg tgagcccgct
     4501 gtcggtcacc caggcgtgat aggcgcgcag aaaatacggc cgttgggaag ggcgcggcgc
     4561 agtcataccg ccgtcgccat cgcccgctcg ctgccggtga gcgagaccac gaaactcggg
     4621 cggcggaaca cccgttgcat gtagccgcgc agcgcccttg cctcgcgcgg gagcggaatg
     4681 ccgagcgcgg gcaggcgcca cagcaccggc gccacgatgc aatcggcgag cccgtagctc
     4741 tcgctcagaa gatacggcgc caccgcgaag acttcgtcgt tcgcgatcag cgtttcgcca
     4801 agtgcgtggc gcgccgccgt cttttcgcgg ctcgtgccct tcgcgatctg acgacactgc
     4861 ccgtaccact cgccgccgac ctcgccgatg aaaagacgga tgcgggcgcg cagcgccggt
     4921 tccgtcgcca tcagcggcgg atgcggatag cgatcgtcca ggtattcggc gatgaccctg
     4981 ggatcgtaga gcgtgacttc gcgggtgacg agcgtcggca tgttgccgta ggggctgagg
     5041 ctcagcagat cgtcgggagg cgcctgcccg ctcacttcga ccagtttcgc cgcgacgccc
     5101 ttctccgcaa ggatcaggcg catcgcgtgc ccggcggggt cgtcgggatg cgagtacagg
     5161 atcatgcgcc ccccgggggg cgtcgtttgc gactcgtcgc gatcaggcgg ggatgcgccg
     5221 gatgtgcgcg ccgagctgcg cgagtttctc ttcaatgcac tcatagcctc gatcaatatg
     5281 ataaatgcga tccaccacgg tttcgccttc ggccaccagc cccgcgagca cgaggctcgc
     5341 cgaggcgcgc agatcggtgg ccatcaccgg cgcccctttg aggttcgtca ccccgttcgt
     5401 gaaggcggtg ttgccttcga gctggattgc cgcgcccatc cgcaccagct cctgaacatg
     5461 catgaaacga ttctcgaaca ccgtctcggt gatcgtgccg gacccttcgg cgaccgcgtt
     5521 gagcgccatg aattgcgcct gcatgtccgt cggaaactcg ggataggggg cggtgcggat
     5581 cgagaccgcc tgcggccggc gcccttccat gtcgagttcg atccagtcac cgtcactcgt
     5641 gattctggct ccggtttccg tgagcttgag gaggatggat tcgaccatcg ccggacgcgt
     5701 gcgtttcacc ttcacgtgtc cgcccgtgag cgcgccggcg acgagatagg tgcccgtttc
     5761 gatccggtcc ggcaggacct cgtagcgcgt tccgtgcagg ctctcgacac cctggatgcg
     5821 gatgacatcg gtgccggcgc cggcaatgtt cgcgcccatc tgtattagac actgggcgag
     5881 gtccaccacc tcgggctcgc gggccgcatt ctcgatcacc gtttcgccgg cggcaagcgt
     5941 cgccgccatc atcaggttct cggtccccgt gaccgacacc gggatcatca ggatccgcgc
     6001 ccccttgagc cgtttggcgt gcgcctgaat gtagccgccc gccacccgga ttttcgcccc
     6061 catggcccgc aggcccttca gatgcaaatc gacgggacgg gaaccgatgg cacagccgcc
     6121 gggaagcgac acgtccgcgc gcccgtagcg ggcgagcatc ggcccgagca cgaggatcga
     6181 agcacgcatg gtccg
//
LOCUS       DASUJG010000334        14987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_705698, whole genome shotgun sequence.
ACCESSION   DASUJG010000334 DASUJG010000000
VERSION     DASUJG010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 14987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14987
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705698"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VFX38_07175"
     CDS             <1..346
                     /locus_tag="VFX38_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314665.1"
                     /translation="FHASDGTLDSNEATITITVALACPDGYVGFAGSLPGGAQRAVTR
                     YQAPAGPQNAILSAAPGIRLGLIYQSPSGQSRTFVFPTPVVHRWGPAGSYTWGVEAGS
                     NGGPYALCLMHP"
     gene            complement(550..1356)
                     /gene="zapD"
                     /locus_tag="VFX38_07180"
     CDS             complement(550..1356)
                     /gene="zapD"
                     /locus_tag="VFX38_07180"
                     /inference="COORDINATES: protein motif:HMM:NF003656.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HEX5314666.1"
                     /translation="MKHADSADQAGADAAPLIFEHPLSERVRSFLRLEAVLAETHRRV
                     AYPEPEINRAALASLIELAALAERGELKREVLTELDRQRLSLAPLQDTSGVDPKRLRN
                     ALGALEQEQARVESTPERPGQNLRANEFLASVRARSTIPGGTCAFDLPALHCWLARPQ
                     EERHKDLERLLSELAPLEWALALLLRHMREAGQALEVIAAGGIYDYMPPSENAPVLLR
                     IGVPAKSAIFPLVSAGKHRITIQFQRWLGIDSRPETLRRDVHFSLALCRL"
     gene            complement(1399..1998)
                     /gene="coaE"
                     /locus_tag="VFX38_07185"
     CDS             complement(1399..1998)
                     /gene="coaE"
                     /locus_tag="VFX38_07185"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014851683.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX5314667.1"
                     /translation="MFTLGLTGGIASGKSEAARVFAGLGVPVIDADRIARDVLAPFSP
                     GLTRLVEKLGSEFLTPAGRLDRQRLRARSFASTELRRRMDEVTHPLILERIRRRLTTL
                     AAPYAVVMAPLLIESGLAREMDRVLVMDCSESLQMARLMERDGETEAGARRMLDAQIP
                     RSERLAAADDVLANDGSFADLAASVRQLHARYLRFASAS"
     gene            complement(2005..2871)
                     /locus_tag="VFX38_07190"
     CDS             complement(2005..2871)
                     /locus_tag="VFX38_07190"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEX5314668.1"
                     /translation="MNGAFPIPGGPVLLAAAGIVGLVVGSFLNVVIHRLPRMIERRWR
                     ADCAELAGEPPAADERYNLVVPRSACPACARPIPAWENIPLLSWIALAGRCRGCRVPI
                     GLRYPAVELLGGGFAVLLALRFGATPAFAGGLLLVWTLIAAAAIDLQHLILPDALTLP
                     LLWAGLLFNLRGTFAPLGSAVIGAVAGYLVLWIVYHAFRLATGKEGIGRGDFKLLAAL
                     GAWFGWQMLPLILLAGAFAGALLGGAWLLAGRRGREHPIPFGPFLAAAGVLALFAGPA
                     IVGAWLAWARPG"
     gene            complement(2927..4132)
                     /locus_tag="VFX38_07195"
     CDS             complement(2927..4132)
                     /locus_tag="VFX38_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX5314669.1"
                     /translation="MATGAREASWRWEGRDHRGERVRGRLTATTLPLARAELRRRGIA
                     PLRVHREHAPRGKRIRASDIAYISRQLATMIAAGVPIAQAFGIIGQGHENRRMRRLCA
                     EIRGDIEAGSSLSQALAKHPRYFDHLYVSLVATGEQSGQLDEILGRIATYKEKTESLK
                     RKIKKALFYPAAVVCAAIIVTAILLIFVIPKFQDFYSSFGASLPAFTRWVIGFSRSVQ
                     NYGWIYLLVVIFLIVLIIQLRKHSPGFRRGMDRLLLRIPVIGTIMRKAAIARFARTLS
                     ITFAAGVPITEALATVARSTGNILYQDAVLQASQRVASGERLHRALEERLIFPNMVTQ
                     MIAIGEESGSVDEMSGKVADFYEEEVDTLVDGLSTLLEPVIIVVLGIIVGALVVAMYL
                     PIFNLGNVL"
     gene            complement(4145..5881)
                     /gene="pilB"
                     /locus_tag="VFX38_07200"
     CDS             complement(4145..5881)
                     /gene="pilB"
                     /locus_tag="VFX38_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEX5314670.1"
                     /translation="MAVAVENLPLSGLARYLVADGMLLLTQIEGAQAEAYRKDIPFIS
                     HLVRERLVEPQMLARRLAEKFGAALADLDAMAIDVECANLVGERIIRAHRVLPFFHRG
                     NRLYVAVSDPTRPQGLDEIRFTTGFVVEPIVCEEDKLERQLSATLEALDTSFAGLDEV
                     VELDEIRGRELEITAENLNDTTIESAEIGKDDAPIVRFVHQLMLLAIQRGASDIHFEP
                     YEQFFRVRMRVDGILREIAKPPAALSPRFAARLKVIAGLDIAERRLPQDGRIKLRYSR
                     NQAMDFRVSSIPTAWGEKVVLRLLDPANAGHGVDALGFEAFQRDLFMEALQRPQGMIL
                     VTGPTGSGKTLTLYTAVQLLNKEGINICTAEDPIEIRLAGINQLQVHPRIDLTFANAL
                     RAFLRQDPDVILVGEIRDLETAEIAVKAAQTGHLVLSTVHTNNAPKTLTRLRDIGVPP
                     YSIASSVRLIIAQRLLRVLCAHCRTPLEIPREALLAEGFDDGDVAAGLRIYGAVGCAQ
                     CTGGYRGRTGVFEMMPVTDEMSYAVMKGEGDADIAKLAREAGVWNLRRSGLAKVKAGI
                     TTLDELHRVTVE"
     gene            6065..7366
                     /locus_tag="VFX38_07205"
     CDS             6065..7366
                     /locus_tag="VFX38_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005003231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HEX5314671.1"
                     /translation="MHLRALRKTLAADLRAGTERGRMMRSAGVTAGLKLGATLLAFGA
                     SLIYARALEPHGYGLYAYVIAWTALLTIPAGLGFSTYLIREGSKAPASLRWLRRWADK
                     RVLLSGIAAGILLACAWFVPQAADARWLFIIAAPLPLLNNLSAVRRALLQARGRVARA
                     QWPLLILGPALMLIALAILWVWRGRLYPIELVAAMTGAAIVPILVNHWQLHRSTRASG
                     SQALEGARVRAALPFMWLGMLYLVNNRADLILLGTLRGAHDAGIYAVASRAAELVTFF
                     LVAANMVLAPRIARLYHEGNLALLQRLLTAAVRRVFLFTLPIALVFIVAAHPLLYYLY
                     GPAYAQGALALQILAGAQLLATSSGPRGVVLNMTGHEKLTAVGVTLSAALNIALNAAL
                     IPLFGVQGSAMATGISIVAFSLLLWYWVRRRVGVNPSALRW"
     gene            7416..8336
                     /locus_tag="VFX38_07210"
     CDS             7416..8336
                     /locus_tag="VFX38_07210"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX5314672.1"
                     /translation="MTEPRIPNLFLIGAPKCGTTALSHYLAGHPDIFMSEQAGSKEPN
                     YFAPDLPFGKLCVESFAEYLALYRRAPQNARYLGDASVRYLRSKVAVPQILEKSPNAK
                     LIVMIRNPIEIAQALHNQRVKRNEDAAVDFEKAWRLQDQRLSSDRDSISTFVSSCAFE
                     YREFARIGTQLARLYNTARRSQVLVIDYGDFARDTARVYQRVLAFLGLEPDGRTSFPI
                     LNARVRYRVPSVQRGLVWLRIIRERLGIPGGWGVQRIADSLNARSDTGSAAGSPLRPA
                     FRAELEQCFKAEVELVSRLQGRDYSHWLRG"
     gene            8370..9557
                     /locus_tag="VFX38_07215"
     CDS             8370..9557
                     /locus_tag="VFX38_07215"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX5314673.1"
                     /translation="MRRASIGKSVRRKTDLAICGAAPAFTEPLHVGRPNIGDRESFLR
                     RVSEIFDDRWLTNNGAMVRQFERRLSEQLNVRNCVAMCNGTIALEIAIRALGLSGEVI
                     VPSWTFIATAHALYWQGITPVFADVDPVTHNLDPEAVRRAITPLTTGIIGVHLWGRPA
                     SVDELQVVANAHELKILFDAAHAFGTSLDGRVIGGFGDCEVLSFHATKVFNTFEGGAV
                     VTNDDELADKMRLMRNFGFSGYDNVIHPGTNGKMAEINAAMGLVNLDALPGFVETNRR
                     NHEDYMRAFEGVEGVRILPHLPEHSPNYQYIVAEVDPEFMASRDEVVSALHAENVLAR
                     KYFWPGCHRMKPYRDLYPHAGLLLPETQKLAARVMVLPTGSSVGTDEIRVIGDVFRVL
                     SRR"
     gene            9573..10724
                     /locus_tag="VFX38_07220"
     CDS             9573..10724
                     /locus_tag="VFX38_07220"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314674.1"
                     /translation="MASAFLVFSGSNDRAVLAFLRVLERCGERPCIVAWTAGDRVFRS
                     RYRRYVVHTRESNVLDEEHFERCIEAVRRNSGTREFVVLPSSEYLNTFLLNHRGAVEA
                     MGCRVPLVSRETYFQLTDKESAARMFAAGGYCIPAEYVSPRELRFPLVAKPRRNLGSG
                     GRLLYPQFVGSAADFSRFQSRFDAPDFFFQEHVCGESFYLLFHLSRSSKTDVVYSQHN
                     LLQQPGGKSILVAAGANLHETEFGRSTVEFLRKLGFHGLGMIEFIQRDGEMVFIEMNP
                     RIWGPSQLCADDSIPMLESFIGEQLDRDAGRYVSRSRRSSLRRSYVWLGGIMQSLCRA
                     RLPVRLGNQPVWPTLIAHGLRDDVYLRRDSWRCFLYDFARAIAGVDNVG"
     gene            10714..11550
                     /locus_tag="VFX38_07225"
     CDS             10714..11550
                     /locus_tag="VFX38_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314675.1"
                     /translation="MSDDLRSELRRLYADASKHSVYQSIPDFVAAELDYFEAIDEGWR
                     SDRPRLAYLLDHRRPEAGEHWADFGANTGFFTLSLARQYSRTLFTAIEANPRHVRFIR
                     LIVAHFDLENVRLVEGSIGLEGLPQLPKCDFLLHLNVLHHAGCDFDGELVPSPVEFGS
                     YASRYLERLRAVSGEIYFQMGSNWGGDKALPLVGARDDEGKLKLFSRWLRLGGWVIDR
                     IAYPSCSGQTGIVYRDFSPGDSPDALGEELRKLDLDQFPGEFYRRPMFLGHRTGFAAP
                     VA"
     gene            11562..12599
                     /locus_tag="VFX38_07230"
     CDS             11562..12599
                     /locus_tag="VFX38_07230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5314676.1"
                     /translation="MDSASTGGALEIGNARTLPEQPIVSVFMATYNQERYIAAAIEGA
                     LAQKVDFPIELVVGEDCSTDGTRAIVLDYQRKHPDLIRIISGSRNAGGFANSNRCLPL
                     CRAKYIAFCEGDDFWHHAEKLKMQVAAMNASPDITLCHTDYNRKIGFRTNRDAHKRHR
                     GALPAEGDAYLSLLHDWTVMTATAMYRADIIRDFLATPFNNPSWPFGDYNKALYASVR
                     GRLAYVPVSTATWRKRMGSATNSGYQRFLAMALAYSECREAFMAEFPPSLEVAESVRR
                     DSRERIMPRAFYAGATDTFEDCRRWLVEHGYARGASRYRLMRAIMGNRPLLATVGKYR
                     ALVQTLGTLGG"
     gene            12639..13028
                     /locus_tag="VFX38_07235"
     CDS             12639..13028
                     /locus_tag="VFX38_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEX5314677.1"
                     /translation="MKRVITFGTFDVFHIGHLSILERAAALGDHLVAGVSTDSLNVAK
                     KGGSPIYPQEERIAIVRALRPVGEAFFEESLEEKGNYIRPYSADVLAMGSDWTGRFDE
                     FHKLCEVAYLPRTPSVSTTVVIERIRR"
     gene            13063..14307
                     /locus_tag="VFX38_07240"
     CDS             13063..14307
                     /locus_tag="VFX38_07240"
                     /inference="COORDINATES: protein motif:HMM:NF016357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HEX5314678.1"
                     /translation="MTTGSRKAIADCLLALASRALAMPLTWLVPRDPRRWLVIGREHG
                     RFLDNAKYFFIWMQQNTADEVTVSFLSERRRAVRDLRAAGARAVSYPGLRAFFALLRS
                     QTVVVDTVDPIEHGRLGFLRGARLVQLWHGAPLKQVERPLYLRQLAATGALARSLLRL
                     YKRIIRRYRPSDVLVSTSTYFTERAFGPCFAARRIVATGYPRNDIVLSGEGYSPALVN
                     INVDQRAADTLARHRAGGGRVVLYAPTFRADMRSPFENGTISLARWSAHAKAHGYVLL
                     LKLHPSMQARSLLAALPSVIALAPESDVYPMLRDTDAMVTDYSSIFFDYLLLDRPIVF
                     FVYDRKRYASEDHGFLFDYDEMTPGPKVESADELFAAIERNLGTAPDSWADARRRVVA
                     LAFDHRDGQASRRLWQALREER"
     gene            14315..14704
                     /locus_tag="VFX38_07245"
     CDS             14315..14704
                     /locus_tag="VFX38_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEX5314679.1"
                     /translation="MKRVLTLGTFDVFHVGHLRILERAAALGDWLVVGVSTDALNVRK
                     KGREPVYPQEERIEIVRSLRCVNEAFFEESLEERESYVRRSRADVLVTGDDRAEYFAG
                     LRGVCEVVVFPRTPSVSTTALIERIRR"
BASE COUNT         2628 a         4709 c         4802 g         2848 t
ORIGIN      
        1 gttccatgcg agcgacggca ccctcgactc gaacgaagcg acgatcacga tcacggtcgc
       61 tctcgcctgt cccgatggtt atgtcggttt cgccggcagc ctgcctggcg gcgcgcagcg
      121 ggcggtcacc cgctaccagg cgcccgccgg cccgcaaaac gcaattctct cggcggcgcc
      181 gggcattcgg ctcggcctca tctaccagag tccgagtgga cagtcccgca cgtttgtttt
      241 ccccacgccg gtcgtgcatc gctggggccc ggcgggcagc tacacctggg gtgtcgaggc
      301 gggttcgaac ggcggcccgt acgccctctg tctgatgcac ccgtagactc gatagcgcgt
      361 tccgcgcaag gaatggggcg ggtgcctaca gcatctgcat cgagcgtccc ctgagggata
      421 ttccggacgg cacgtcgcgg gccggcgtgt ttcggcaccg caatcgcgaa cagcgacgca
      481 gcttcgtgtg cgatgaagcg atcgttgcga ccggggacgg ctcgggaatc cgagacgtct
      541 ccgcgaacgc tacagccggc agagcgcgag cgaaaagtgc acgtcgcggc gcagcgtttc
      601 ggggcggcta tcgatcccga gccagcgctg gaactggatc gtgatgcggt gcttgcccgc
      661 actcacaagc gggaaaatcg cgctttttgc cggcaccccg atgcgcagca ggaccggcgc
      721 gttttcactc ggcggcatgt aatcgtagat gccgcccgcg gcgatcacct cgagcgcctg
      781 cccggcctcg cgcatgtgcc ggagcaggag cgcgagcgcc cactccagcg gcgcgagttc
      841 cgacagcagc cgctcgagat ccttgtgacg ctcctcttgc gggcgggcga gccagcagtg
      901 gagcgccggc aggtcgaagg cacaggttcc cccggggatg gtgctccgcg cccgcacgga
      961 ggcgagaaat tcgtttgcgc gcagattctg tccgggccgc tcgggcgtcg attcgacccg
     1021 ggcctgttcc tgttcgaggg cgccgagcgc attgcgcagg cgcttcggat ccacgccgct
     1081 cgtgtcctga agcggggcga gcgaaagccg ctggcgatcg agttccgtga gcacttcccg
     1141 tttgagttcg ccgcgttcgg cgagggccgc gagttcgatc aggctcgcga gcgccgcccg
     1201 gttgatctcc ggctcggggt aggcgacgcg acggtgcgtt tccgccagca ccgcctcgag
     1261 ccgcagaaaa ctgcgcaccc gctccgagag cggatgctcg aatatcagcg gggcggcgtc
     1321 cgcccctgcc tggtccgcgg aatcggcgtg cttcatcgcc acttgattct atcagcggcg
     1381 ggaggatcgt tcgttcgctc agctcgcgga cgcaaaacga agataccggg cgtggagctg
     1441 acgaacgctc gcggcaaggt ccgcaaacga cccgtcgttg gccaaaacgt cgtcggccgc
     1501 agcgagccgt tccgatcgcg gaatttgcgc atcgagcatc cgccgcgcgc ccgcctcggt
     1561 ctcgccgtct cgctccatga gccttgccat ttgcagggat tcggagcaat ccatcaccaa
     1621 aacccgatcc atctcccgcg ccagtcccga ctcgatcaga agcggcgcca tgacgacggc
     1681 atagggcgcg gcgagcgtcg tgagacgccg gcgaatccgc tccagaatca agggatgggt
     1741 cacctcatcc atgcgccgac gcagctccgt cgaggcgaag ctccgcgccc gcagccgctg
     1801 gcggtcgagc ctgcccgcgg gcgtcaggaa ctccgaaccg agcttttcga ccagccgagt
     1861 caatcccggt gaaaacgggg caagcacgtc gcgggcgatg cgatcggcgt cgatgaccgg
     1921 aaccccgagg ccggcgaaga ccctcgccgc ttccgatttg ccgctggcga tgccgcccgt
     1981 aagcccgagc gtgaacatct cgtcctatcc cggccgcgcc caggcaagcc aggcccccac
     2041 gatggcgggc ccggcgaaaa gcgcgagcac gccggcggcg gcgagaaaag gtccgaaggg
     2101 gattggatgc tctcgcccgc gccgcccggc gagcagccag gcgccgccga gaagcgcgcc
     2161 ggcgaaggcg cccgcgagga gaatcagcgg cagcatctgc cagccgaacc aggcacccag
     2221 cgcggcgagc agcttgaagt cgccccggcc gatgccttcc ttgccggtcg cgaggcgaaa
     2281 ggcgtgatag acgatccaga gcacgaggta gccggcgacg gctccgatca cggccgagcc
     2341 gagcggcgca aaggttccgc gcagattgaa caagaggccg gcccacagga gcggcagggt
     2401 gagcgcatct ggcaggatga gatgttggag atcgatcgcc gccgccgcga tcagcgtcca
     2461 tacgaggagc aggccgccgg cgaaggcggg cgtcgcaccg aagcggagcg caagaagcac
     2521 cgcgaagcca ccgcccagga gttccaccgc ggggtaacgc aatccgatcg gcacccggca
     2581 gccgcggcag cgcccggcga gcgcgatcca gctcaggagc gggatgtttt cccaggcggg
     2641 gatgggccgc gcgcatgccg ggcaggccga gcgcggcacc accaggttgt agcgttcgtc
     2701 ggctgccggc ggttcgcccg cgagttccgc gcagtccgcg cgccagcgcc gctcgatcat
     2761 gcgcgggagg cggtggatca ccacgttgag gaagcttccg acgacgagcc cgacgatgcc
     2821 ggccgccgcc agcaacacgg ggccgccggg gatcgggaac gcgccgttca tcgtcgcgtt
     2881 cgggacatcg gcggcacgta caaaaacact ggtgtccgcg gagtgcctag agcacgttgc
     2941 cgaggttgaa gatgggcaga tacatggcga cgacgagcgc tccgacgatg atgcccagga
     3001 ccacgatgat gaccggctcg aggagcgtgc tcaaaccgtc caccagggtg tccacttctt
     3061 cctcgtaaaa gtccgcgacc ttgccggaca tctcatccac cgaaccggat tcctcgccga
     3121 tggcgatcat ctgggtcacc atgttcggga agatgaggcg ctcctcgagc gcgcgatgca
     3181 ggcgctcgcc gctcgcgacc cgctggctcg cctgcagcac cgcatcctga tagaggatgt
     3241 tgccggtcga gcgggcgacg gttgcgagcg cctcggtgat cggcaccccg gcggcgaagg
     3301 tgatcgagag cgtgcgcgca aagcgcgcga tcgcggcctt gcgcatgatc gtcccgatca
     3361 ccgggatgcg cagcagcagc cggtccatgc cgcggcggaa tcccggggag tgcttgcgca
     3421 actggatgat cagcacgatc aggaagatga cgaccagcag atagatccag ccgtaattct
     3481 gaaccgagcg cgaaaagccg atcacccagc gggtgaaggc cgggagcgag gcgccgaaac
     3541 tcgaatagaa atcctggaac ttcggaatca cgaagatgag caggatcgcg gtgacgatga
     3601 tcgccgcgca gactaccgcg gcggggtaga acagcgcctt cttgatcttg cgcttgagcg
     3661 attcggtctt ctccttgtaa gtggcgatgc gcccgaggat ctcgtcgagc tggccggact
     3721 gttctcccgt tgcgaccagg ctcacgtaca gatgatcgaa gtagcgcgga tgtttcgcga
     3781 gtgcctggga gagcgaactt cccgcctcga tgtcgccgcg gatttccgcg cacaggcgcc
     3841 gcatgcggcg gttctcgtgt ccctgaccga tgatgccgaa ggcctgcgcg atcggcactc
     3901 ccgcggcgat catggtcgca agctggcggc taatgtaggc gatgtcgctc gcgcggatgc
     3961 gtttgccgcg gggcgcgtgc tcgcggtgca cgcgcagcgg cgcgatgcca cggcggcgca
     4021 gctccgcgcg cgcgagcggc agcgtcgtcg ccgtgaggcg gccgcgcacg cgctcgccgc
     4081 gatgatcgcg tccctcccag cgccaactcg cttctctcgc tcccgtggcc atggcgaagc
     4141 ctccttactc gaccgtaacc cgatggagtt cgtcgagcgt ggtgatgccc gccttgacct
     4201 ttgcgaggcc cgagcggcgc agattccaaa ctcccgcctc ccgggcgagc ttcgcgatgt
     4261 cggcatcgcc ctcgcccttc atcaccgcgt agctcatctc gtccgtcacc ggcatcatct
     4321 cgaagacgcc ggtgcggccg cggtacccgc cggtgcactg ggcgcagccg acggcgccgt
     4381 aaatccgcag gcccgcggcg acatccccat catcgaaacc ttcggcgagg agtgcctcgc
     4441 gcggaatctc gagcggcgtg cggcaatgtg cgcacagcac ccgcagcagc cgctgggcga
     4501 tgatcaggcg cacgctcgag gcgatcgaat acggcggcac accgatatcg cgcaggcgcg
     4561 tcagggtctt cggcgcattg ttcgtgtgca cggtcgagag cacgagatgc ccggtctggg
     4621 ccgccttcac cgcgatctcg gcggtctcca gatcgcggat ttcgccgacc aggatcacgt
     4681 cggggtcctg gcgcagaaag gcccgcagcg cgttcgcgaa ggtcagatcg atgcgcgggt
     4741 gcacctgaag ctgattgatg ccggcgaggc ggatctcgat cggatcctcg gcggtgcaga
     4801 tgttgatgcc ttccttgttc aacaactgca cggcggtgta aagggtgagc gtcttgccgc
     4861 ttccggtcgg gcccgtgacg agaatcattc cctgcggccg ctggagcgct tccatgaaga
     4921 gatcgcgctg aaaggcctcg aagccgaggg cgtcgacgcc gtggccggcg ttcgcgggat
     4981 cgagaaggcg cagcacgacc ttctcgcccc aggccgtggg aatgctgctc acgcgaaagt
     5041 ccatcgcctg gttgcgcgaa tagcgcagct tgatgcgtcc gtcctgcggc aggcgtcgct
     5101 cggcgatgtc gagccccgcg atcaccttca ggcgcgccgc gaaccttggg ctgagcgcgg
     5161 ctggcggctt ggcgatttcg cgaaggattc cgtccacgcg catgcgcacg cggaaaaact
     5221 gctcgtaggg ttcgaaatgg atatcggaag cgccgcgctg gatcgcaagc agcatgagct
     5281 gatgcacgaa gcgcacgatc ggcgcgtcgt ccttgccgat ctcggcgctt tcgatcgtgg
     5341 tgtcgttcag attctccgcc gtgatttcga gttcgcgccc gcggatctcg tcgagttcca
     5401 cgacctcgtc gaggccggcg aagctggtgt cgagcgcctc gagggtcgcg gaaagctgcc
     5461 gctcgagctt gtcttcctcg cagacgatgg gctcgaccac gaagccggtg gtaaagcgga
     5521 tttcgtcgag cccctgcgga cgggtgggat cggacaccgc gacatacagg cggttgccgc
     5581 ggtggaagaa cggcagcacc cgatgcgcgc gaatgatgcg ctcgccgacc agattcgcgc
     5641 attcgacgtc gattgccatc gcgtcaagat ccgcgagcgc cgcaccgaac ttctcggcga
     5701 ggcggcgcgc gagcatctgc ggctcgacca agcgctcgcg caccagatga ctgatgaagg
     5761 gaatatcctt gcggtaggcc tccgcctgcg cgccctcgat ctgcgtgagc aacagcatcc
     5821 cgtcggcgac gagataacgc gcaagtccgc taagaggcag attttctacg gcaacggcca
     5881 tgaactgccc ataccctccg gcgccggtac tttagaccaa aacggggcca tcgtacaggg
     5941 gcagggactt ctgcgccgcc cgcatcccgg gaaggttgcc gattgtgatc gcggctgatc
     6001 ggcgcggcgg gcgcgggaat aaaccgataa gatgcgtgcg atccgtgctt cggtagcctc
     6061 tccggtgcat ctgcgagccc tgcgcaagac tctcgccgcc gatcttcgcg ccggcaccga
     6121 gcgcggccgg atgatgcgct ccgccggcgt caccgcgggg ctcaagctcg gagcgacgct
     6181 tctcgccttc ggcgcgagct tgatctatgc gcgggcgctt gagccgcacg gctacgggct
     6241 ctatgcctac gtcatcgcct ggacggcgct cctcaccatt cccgccgggc tgggtttttc
     6301 gacctacctg atccgcgaag gatcgaaggc gccggcgagt ctgcgctggt tgcgccgctg
     6361 ggccgacaag cgcgtgcttc tcagcggcat cgccgccggc attcttctcg cctgcgcctg
     6421 gttcgttccg caggcggccg atgcccgctg gctcttcatc atcgcggcac cgctgccgct
     6481 cctcaacaat ctgagcgccg tgcgccgtgc gctgctccag gcgcgcggcc gcgtagcccg
     6541 cgcccagtgg ccattgttga ttctcggccc ggcgctgatg ctcattgcgc tcgccattct
     6601 ctgggtctgg cgcgggcgtc tctacccgat cgaactggtg gccgcgatga ccggcgccgc
     6661 catcgtgccg attctcgtca atcactggca gcttcaccgc tcgacccgcg ccagcgggag
     6721 ccaggctctg gaaggcgcac gggtgcgcgc agccctgcct ttcatgtggc tcggcatgct
     6781 gtatctggtc aacaatcgtg ccgatctcat cctgctgggg acgctccgcg gcgcgcacga
     6841 cgccggcatc tacgccgtcg cctcgcgggc cgccgaactc gtgaccttct ttctcgtcgc
     6901 cgccaacatg gtgctcgcgc cgcggatcgc gcgcctctat cacgaaggca atctcgccct
     6961 cctgcagcgc cttctgaccg ccgcggtgcg ccgagtattc ctcttcaccc tgccgatcgc
     7021 cctcgtcttc atcgtcgcgg cacatccgct gctctactac ctctacggcc cggcctacgc
     7081 gcagggagcc ttggcgcttc agattctcgc cggagcgcag cttcttgcga cctcgagcgg
     7141 cccccgcggc gtggtgctca acatgaccgg ccacgagaaa ctcacggccg tcggggtgac
     7201 cctgagcgcc gctctcaaca tcgcgctcaa cgccgccctg atcccgcttt tcggcgtgca
     7261 gggctcggcg atggcgaccg gcatcagcat cgtcgccttc agcctcctgc tctggtactg
     7321 ggtccgccgc cgggtcggtg tgaatcccag cgccctgcgc tggtaagcgg cgacctgcga
     7381 taatctcagg catcctgcca tagtgctcga agcccgtgac cgagccgcgc atccctaatc
     7441 tcttcctcat tggtgcaccc aaatgcggta ccacggcact cagccactat ctcgccggtc
     7501 atcctgatat cttcatgtcg gagcaggccg ggtcgaagga gccgaactac tttgctccgg
     7561 atctgccgtt tggcaagctt tgcgtcgagt cattcgcaga atatctcgcc ctgtaccgcc
     7621 gcgctcccca aaacgcgcgt tacctgggcg acgcttcggt ccgctacctg aggtcgaaag
     7681 tcgccgttcc acagatcctc gagaagtctc cgaatgcgaa gttgatcgtc atgattcgca
     7741 atcccatcga gattgcgcag gcgttgcaca accagcgagt gaagcgcaac gaagacgcgg
     7801 cagtggattt cgaaaaggcc tggcgcctgc aggaccaacg cctctcgtcg gatcgcgatt
     7861 ccatcagcac cttcgtctct tcctgcgcgt tcgagtatcg tgagttcgcg aggatcggga
     7921 cgcagttggc gaggctttac aatacggcgc gaagatcgca ggtgctggtg attgactacg
     7981 gagattttgc ccgtgatacc gctcgcgtct atcaacgcgt gctggccttt ctcgggctcg
     8041 aaccggatgg gagaacgagt ttccccatcc tcaatgcgag ggtgcgttat cgcgtccctt
     8101 cagttcagcg tgggctggtc tggctgagga ttattcgtga acgactggga attcctggag
     8161 gttggggcgt ccagaggatt gcggactctc tcaacgctcg ctccgacact ggaagcgcag
     8221 ccggctctcc gttgcgtcca gcgtttcgcg cggaacttga gcagtgtttc aaagccgagg
     8281 tggaactcgt ttcgagattg caagggcgcg attattctca ctggttgaga ggttgaatga
     8341 cgctcatgaa aactggatcg gcggaatcga tgcggcgtgc ttcgatcgga aagtccgttc
     8401 ggcgcaagac cgatctggcg atttgcggtg ctgcgccggc gttcacagaa ccgcttcatg
     8461 taggcagacc caacatcggc gatcgtgagt cattcctccg gcgggtaagc gagatattcg
     8521 acgatcgctg gctgacgaat aacggtgcga tggtgcgcca attcgagcgg cggctctccg
     8581 aacaattaaa cgtccgcaat tgcgtcgcta tgtgcaacgg aacgattgca ttggaaatcg
     8641 cgattcgggc gctcggtctg agcggcgagg tcattgtgcc gtcgtggact ttcatcgcga
     8701 cggcgcatgc gctctactgg caggggatca cgccggtttt tgccgacgtg gacccggtga
     8761 cgcacaatct cgatcccgag gccgtccgcc gggcaattac gccgctcacg acggggatca
     8821 tcggcgtgca tctatggggc cgcccggcgt ccgtggacga gctccaagtc gttgcgaacg
     8881 cgcatgaatt gaaaatcctg ttcgatgcag cgcacgcttt cgggacgagc ctcgatggcc
     8941 gcgtgatcgg tggtttcggc gattgcgagg ttctgagttt ccacgcgacg aaggtcttca
     9001 acacgttcga gggtggggct gtcgtaacca acgacgatga actggcagac aagatgcgcc
     9061 tcatgcgcaa cttcggcttc agcgggtacg acaacgtgat tcaccccgga accaacggga
     9121 aaatggcgga gatcaatgcc gcgatgggct tggtcaatct ggacgcgctt ccggggttcg
     9181 tcgaaaccaa tcgccgcaac catgaagact acatgcgcgc attcgagggc gtcgaaggcg
     9241 tccggattct tccgcatctc cccgaacatt caccgaatta ccagtacata gttgcggagg
     9301 tagacccgga gtttatggca agccgtgacg aggtcgtctc cgcgctgcac gccgagaatg
     9361 tgctggcacg caaatatttt tggccaggat gccatcggat gaagccgtat cgggatctct
     9421 acccgcatgc agggctgtta ctgcccgaaa cccaaaagct tgcggcccgc gtcatggtgc
     9481 tcccgaccgg aagctccgtg ggcacggacg agattcgcgt aatcggcgac gtctttcgcg
     9541 tcctctcccg ccggtgatag ccggatagac cggtggcatc cgcgtttctc gtgttcagcg
     9601 gcagcaacga ccgcgcggtg ctggcgtttc tgcgcgtcct tgaacgttgc ggcgaacgtc
     9661 cctgcatcgt tgcctggacg gccggcgatc gcgtcttccg ttcccggtat cgccgatacg
     9721 tcgtccacac acgggagagc aacgttctcg acgaggaaca ctttgaacgt tgcatcgaag
     9781 cggtgcggcg caattccgga acgcgggaat tcgtcgtgtt gccgtcctcc gaatatctca
     9841 atacgtttct attgaatcat cgaggtgccg tcgaggcaat ggggtgtcgg gtaccgctgg
     9901 tgtcgcgtga gacctacttt caacttaccg acaaggaaag cgccgcccgg atgttcgcgg
     9961 ccggcggcta ttgcatacct gccgaatacg tctctccccg agaactgcga tttccgctcg
    10021 ttgcgaagcc gaggcgcaat ctgggatccg gcgggcgatt gttgtatccg caattcgtcg
    10081 ggtcggcggc cgatttctcg cggtttcagt cacggttcga cgcgccggat ttcttctttc
    10141 aggaacatgt atgcggagag agcttctatc tcctgttcca tctttcgcga tcgtccaaaa
    10201 ccgacgttgt ctattcgcag cacaatctcc tccagcaacc gggagggaaa tccattctcg
    10261 tcgccgcagg ggcgaatctt catgagacgg aatttggcag gtccaccgtg gaatttctgc
    10321 ggaagctcgg cttccatggc ttggggatga tcgagttcat tcaacgggac ggagagatgg
    10381 ttttcatcga gatgaacccg cgaatctggg gtccgtctca gctttgtgcg gacgatagca
    10441 tcccgatgct ggaatccttc ataggtgaac agctcgatcg cgatgcgggg cgttatgttt
    10501 cccggagccg gcggagcagc ctgcgacgct cctacgtctg gctgggcggg atcatgcaga
    10561 gcctgtgccg cgcgagattg cccgttcgct tggggaatca acccgtctgg ccgacattga
    10621 tcgcgcatgg actgagggac gacgtgtatc tgcggcgtga ctcatggcgc tgcttcctgt
    10681 acgattttgc acgggcaatc gccggagttg ataatgtcgg atgacctaag aagtgagctg
    10741 cgccgcctgt acgctgatgc ctccaagcac tccgtctatc agagcatccc ggactttgta
    10801 gccgcagaac ttgattattt cgaggccatt gacgaagggt ggcgaagcga ccgcccccgt
    10861 cttgcttacc tgcttgatca tcgcagaccc gaggcaggcg agcattgggc ggacttcggc
    10921 gccaataccg gtttcttcac gctcagcctc gcccgacaat attcccgcac gctgtttacg
    10981 gcgatcgaag cgaatccccg tcacgttcgc ttcattcgac tgatcgtcgc ccacttcgat
    11041 ctcgaaaacg tgcggttggt ggaaggatcg atcggtttgg aaggtcttcc ccagcttccg
    11101 aaatgcgatt tcctgttgca tctcaatgtg cttcatcacg cggggtgcga cttcgatggg
    11161 gagctggttc cgagccctgt agagttcggg agctacgcaa gccgctatct ggaaagactc
    11221 cgcgccgtgt ccggcgagat ctatttccag atgggaagca actggggagg cgataaagct
    11281 cttcccctgg tgggggctcg tgacgatgag gggaaattga agctattctc gcgctggctc
    11341 cgcctgggcg gatgggtgat cgaccggatc gcatatcctt cttgcagcgg ccagaccgga
    11401 atcgtctacc gggacttctc tcccggcgat tcgccagacg cgctcggcga ggaacttcgc
    11461 aaactcgatc ttgaccagtt tcccggagag ttttatcggc gtcccatgtt ccttgggcac
    11521 cgaaccgggt tcgcagcacc agtcgcgtga ccggcgagag gatggattcg gcttcgacgg
    11581 gcggagctct ggaaataggc aacgccagga cgcttccaga gcaacctatc gtatcggtgt
    11641 tcatggcgac atataaccag gagcgctaca tagccgcggc gattgagggg gctttggcac
    11701 agaaagtgga ttttcccatc gaactcgtcg tcggagagga ctgttcaacc gacggaacgc
    11761 gcgcgattgt ccttgattac caacggaaac atcctgacct gatccggatc atttccggtt
    11821 cgcgcaatgc agggggattt gccaactcca atcgttgcct tccgctctgc agagcgaagt
    11881 acatcgcctt ttgcgagggt gacgactttt ggcatcatgc agaaaagcta aaaatgcagg
    11941 ttgctgccat gaacgcatcg ccggacatca ccctctgcca caccgactat aatcgcaaaa
    12001 tcggatttcg gacgaaccgc gatgcgcaca agcgtcaccg cggagcactg cccgcggagg
    12061 gcgatgccta cttgagtctg cttcacgact ggacagtgat gacagccacg gcaatgtacc
    12121 gggccgatat catccgggac tttctcgcga ctccgttcaa caatccgtcg tggccgttcg
    12181 gggattacaa caaggcgctc tacgcgagcg ttcgcggtcg gctggcatat gttcctgtct
    12241 ccacggccac ctggagaaaa cgcatgggtt cggcgacgaa ttcaggctac cagcggtttc
    12301 tggcaatggc tctggcatac tcggagtgcc gcgaagcatt catggcagaa tttccgccat
    12361 cgctggaggt tgcggaaagc gtgcgccgcg acagccgcga acgcatcatg ccgcgggcct
    12421 tctatgccgg cgcgacggat accttcgagg attgccgtcg ctggttggtg gagcatggtt
    12481 acgcccgcgg cgcaagccgc taccgcttga tgcgagcgat tatggggaac agaccgctgt
    12541 tggcgaccgt cgggaaatat cgcgcgctgg ttcagacgct cgggacgctc ggcggttgat
    12601 ctgccggaag atgtggcgtt agcaacaggg agacgattgt gaagcgcgta attacattcg
    12661 gcaccttcga tgtcttccat atcggccact tgagcattct cgaaagggcg gcggcgctcg
    12721 gggatcatct ggtcgccgga gtctcgaccg attccttgaa cgtcgcgaag aaggggggat
    12781 cgccgatcta tccgcaggag gagcggatcg cgatcgtacg tgcattgcgc ccggttgggg
    12841 aggcattttt cgaggaatcg ctcgaggaaa aaggaaacta catccggccc tatagcgccg
    12901 atgtgctggc tatgggaagt gactggacgg gccgtttcga cgagttccac aagctctgcg
    12961 aggtcgctta tctgccgcgc actccttcgg tttctaccac cgtcgtgatc gagaggatcc
    13021 gacgttgaag gattcctctt cgatgccatc gcgcccgcac gcatgacgac aggcagccgc
    13081 aaggccattg ccgattgtct cctcgcgctc gcatcgcgtg cacttgctat gccgctgaca
    13141 tggctcgtgc cacgcgaccc gcggcgatgg ctggtgatcg gacgcgagca tggacgtttt
    13201 ctcgacaacg ccaagtattt tttcatctgg atgcagcaga atactgcgga tgaagtgacc
    13261 gtcagcttcc tcagcgaacg gcggcgagcg gtgcgcgatc tgcgggctgc tggcgcccgc
    13321 gctgtttcct atccgggtct gcgcgccttc ttcgcccttt tgcgttcgca gacggtggtc
    13381 gtggacacgg tggacccgat cgaacacggt cgcctgggat tccttcgcgg cgcgcggctc
    13441 gtccaactct ggcacggcgc acctttgaag caggtcgaac gtccgctgta tctccggcaa
    13501 ctcgcggcga ctggcgcact ggcgcggtcg ttactccgct tgtacaaacg catcatcagg
    13561 cgttatcggc cgagcgacgt tctggtgtcc acgtcgacct atttcaccga gcgtgccttc
    13621 ggcccgtgtt tcgccgcgcg gcgcatcgtc gcaaccggat atccgcgcaa cgacatcgtt
    13681 ctgagcggcg aaggatattc tccggcgctt gtgaacatca acgtggatca gcgggcagcg
    13741 gacacactgg cgcggcatcg ggctggcggt gggcgggtgg tgctttacgc gccgactttt
    13801 cgcgcggaca tgcgttcgcc gttcgagaat gggacgattt cactcgcgcg ctggtcggcg
    13861 cacgccaagg cgcatggcta cgttctactg ctgaaactcc acccttcaat gcaagcccgg
    13921 tcgcttttgg cagcattgcc ttccgtcatc gctcttgcac ccgaaagcga cgtttatccg
    13981 atgctccgcg acacggacgc catggtaacg gattattcct ccattttttt cgactatctg
    14041 ctgctcgatc ggccgatcgt gtttttcgtc tacgaccgca aacgctatgc gtcggaggac
    14101 cacggttttt tgttcgatta cgacgagatg actccaggcc cgaaggtcga aagtgccgac
    14161 gagcttttcg ccgctatcga acgaaacctg gggaccgcgc ccgattcctg ggcggacgcg
    14221 cgccgccgcg tcgtcgcctt ggccttcgat catcgggacg gtcaggcttc ccggcgcctg
    14281 tggcaggcct tgcgcgagga gcgctgataa cgcgatgaag cgcgtgctca cgctcgggac
    14341 cttcgacgtg tttcacgtcg ggcatctgcg cattctcgag cgcgcggcag cgctcggaga
    14401 ctggctcgtc gtcggcgttt ccaccgatgc actcaacgtc cggaagaagg ggcgcgagcc
    14461 ggtctatccg caggaagaac ggatcgaaat cgtgcggtcg ctgcgctgcg tgaacgaggc
    14521 gtttttcgag gaatcgctgg aggaacgcga aagctacgtg cgccgctcgc gcgcggacgt
    14581 tctcgtcacg ggtgacgacc gtgccgagta cttcgcgggt cttcgtggcg tctgcgaagt
    14641 cgtggtgttc ccgcgtactc cttcggtgtc cacaaccgcg ctgatcgagc gcatccgccg
    14701 ctgataacag ttgtggcatt gctgatggag tctggtatcg tcattccgga accgcgacgc
    14761 ggtgtgcgga atccggtacg aagataacga atcccgctct ggggacggga tacatgatct
    14821 tgactgggtc ccggcctgcg ccgggacgac ggcgttgcgc tagccggcgc ggaatccgag
    14881 caacgcggcg gtgcgtgcgg cgatttccgg ccagttcatc tccgatgcgg gcggcgcgat
    14941 gcggcgggcg gcgagttgcg cgcgaatggc ctgcgcgagg ccggacg
//
LOCUS       DASUJG010000335         9892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1318138, whole genome shotgun sequence.
ACCESSION   DASUJG010000335 DASUJG010000000
VERSION     DASUJG010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9892
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318138"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1773
                     /locus_tag="VFX38_07250"
     CDS             <1..1773
                     /locus_tag="VFX38_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009573145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HEX5314680.1"
                     /translation="DASDTGQGIFRVRETIPVQPGELTLLYPKWIPGNHSPSGPIDKL
                     AGLTITAGGKPLAWKRDKYDVYAFRIDVPKGVAKIDLDYQFLSARGGNEGPIQMTDTM
                     LDLVWSKLALYPAGHYTRDISYRASVKLPQGWQFGTALTKQSQSGDTVTFEPTTFNTL
                     VDSPLYAGLYYKRLDLDPGSKVPVHMDIFADAPKYLVVTPTQLKKLRALVTQAYLLFD
                     SQHYDHYDFLFSLSDQLSGKGLEHHRSTEVGTMADFFTDWDKRAPGRDLLAHEYTHSW
                     NGKFRRPADLWTPNFNVPMGDSLLWVYEGQTQYWGYVLTARSGMWSQQQYRDALALVA
                     ATYDRNRPGFEWRGVQDTTNDLTIAQRAPLPYRSWQMSEEYYSAGQLIWLAADAKIRA
                     LTQDKKSLDDFASAFFGVDNGSFVTKTYTFDDVVAALNGVVKYDWEKFLRSRLDAHAP
                     PLDGIAASGWKLVYSDKESDYQKQLSKVTHRPQNFAFSIGLALNKDGGIYDVRWNGPA
                     FKAGVSAGATLVAVNGHAYKPEVLKDAITAAKSDKAPIELLLKYQDEYRTVPVDYHGG
                     LQYPHLVRIKGTPDYLDQIITARK"
     gene            2060..2701
                     /locus_tag="VFX38_07255"
     CDS             2060..2701
                     /locus_tag="VFX38_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314681.1"
                     /translation="MNKSGNIFAVMAGGALAAGLALSPAFALGSNTDNLQGARAKQSQ
                     KYEDLTQAKVQKVIATYKKDTGALENYFNDAYGYVVFPSIGVGSNVVGNDHGTGAVYR
                     DGVLVGHSTVVKLPMNVETGGQTFSEMIFFQDKRAFELFTEDKYHFHGKTTAADVRGG
                     LTPSTQYSHGMAVFINMKSGAETTAQNDKIKIGAQEFMFDPIGAEQNPSATHP"
     gene            complement(2823..3761)
                     /locus_tag="VFX38_07260"
     CDS             complement(2823..3761)
                     /locus_tag="VFX38_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5314682.1"
                     /translation="MRSTLRPLFAILAAALLASACATTPSGPAAFTVGTLTVHRYGDH
                     GRPLILIPGLGSGAWVWDGTVAQFEGDHVIYVLTLAGFDGTPLPANHENLMGQADASL
                     LELIRSHNIDHPVLVGHSLGGTLALQFASEHADLLSGVVAVDGLPVFPGTQDMSAEQR
                     AAIGRGMSARMAALSPEQFREQQLAYMQTIGLIDPAMAAKYAALQDQSDPAAVAEYAG
                     EDLALDLRPQLKNIGVPVLEISPYNPPDFAKDSKLPISEAQKTAYYQMLLAGIPNVEV
                     ESISPSRHFAMLDQPQKFYDILNTFLASLQATQPSP"
     gene            complement(3882..4814)
                     /locus_tag="VFX38_07265"
     CDS             complement(3882..4814)
                     /locus_tag="VFX38_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX5314683.1"
                     /translation="MTVSIVVAIILVILALTILSRGLRIVPQQHAWVIERLGRFQRIL
                     EPGLNLIIPIADKVAYRFDLRETPIDVRKQVCITKDNTQISIDGVLYLQITDPKAAAY
                     GTSNPLSSVEQLAQTIMRSDVGKKQLDEVLSSRTELNASIVNELDRAAVNWGVKVLRY
                     EIRDISPPEEVIRAMELQITAERQKRALIAQSEGEKQQAINVSEGERQQQINRAEGEK
                     QAQVLRAEGEAQAVLTVADATAKALATVGAALRQDGAGEAMRMQVAQDFIAQWGGIAK
                     ASNVMIVPADMGDLSKVVGTAFRIVNGAPATSGK"
     gene            complement(4854..5303)
                     /locus_tag="VFX38_07270"
     CDS             complement(4854..5303)
                     /locus_tag="VFX38_07270"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX5314684.1"
                     /translation="MRSETVLGIVLLVIGAIATVAEIHSLTIYLIAVAVACFAAAGVA
                     FAGGGLDVALIVLAGVILFGLPVAHWARGRLKNRASDEITADDIGNRVTIVASDGGVL
                     RVAYRGSNWDARLDQAAAATPQIGDNYRIVARDGNVLVLASAPDGRA"
     gene            5667..6353
                     /locus_tag="VFX38_07275"
     CDS             5667..6353
                     /locus_tag="VFX38_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014036071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314685.1"
                     /translation="MNDAALERLREWEGLTRDYARFSRSAGGLGSALGGVLTLVSYFA
                     GALLPSDSAARLGLIAIPFVWLLVRGALARRYYQRFGHVEEQESTAQRRTHRFCVAVT
                     LLVAAGITAVTAGEVTTKTGADLAGLIGYLALIWFLVLATWRWLRSPLDFIVGVFLFC
                     QAALICVGRSYPLIWLTHTREALLMSLIAAMFPLAALAMIAAGIGDHRRFLRLRTRLA
                     ELQRTAVNRS"
     gene            6350..6691
                     /locus_tag="VFX38_07280"
     CDS             6350..6691
                     /locus_tag="VFX38_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX5314686.1"
                     /translation="MSGESVPETLLGLDRLVHEPARLLILSVLAGAKEVEFGFLEKMS
                     GLSKGNLSSHMSKLEAAGLIGVRKRFRAKRPLTELHLTAKGRRALEAYRRQLATVMRS
                     LPAEPADGKRR"
     gene            6748..8451
                     /locus_tag="VFX38_07285"
     CDS             6748..8451
                     /locus_tag="VFX38_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5314687.1"
                     /translation="MKNCFTFVPGILSVALTALVTSLLPSACAAAAPPAAVSSLLPAR
                     VLQAARDRVAAGEYPALVIGVVDGGRSRLYSFGKLANGEAPDAKTVFEIGSITKTFTA
                     LLLAEAAKEGSVALAEPVAKLLPHFSIPSRDDRQITLEDLADQHSGLPRLPTNLDPAN
                     PADPYAGYDADRLKAFLASYELPRDPGSAYEYSNLGFGLLGYALARHADMTYDALVEE
                     RILQPLGMKRSGVAMTAAMRAQFAAGHDATGKSAANWDFGVLAGAGALKSDGADMLRY
                     LEANMGLRKSFLSAAMRLAQKPLLAANGKERVGLAWMTLPTDRGDVVWHNGMTGGYAS
                     FIGFTADGKRGVVILTNIQQSVDDLGFATLLADWPLAPAEKAITLPADELDDYAGSYR
                     LAPKFVLTIFLQGDQLYAQATGQGPFPIFPSAKDEFFARVSDIRISFERDAQGKVTDL
                     VLHQNGDRRAPLIGAAQAAAAATSTPIVALPAATLRQYVGQYQLAPGAVFDVTLAGDQ
                     LSVRLSGQPAFPVYASAKDRFFYTVVDARIEFVRDDEGNVVALVLHQNGQARRAPRVQ
                     Q"
     gene            8458..9372
                     /locus_tag="VFX38_07290"
     CDS             8458..9372
                     /locus_tag="VFX38_07290"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX5314688.1"
                     /translation="MDPRCFFVIGAQKGGTTALFEYLRGHPEVYMPPEKEAEFFTRCD
                     TTPAAWAEYRKRLLTPPRPVRVVGTASPQYMGDPAVPARMAAVVPDALLIAVLRDPVE
                     RAVSHYKMAYRRGEERRDFARAVREQLQPEALEKARALPFREVAERDAYVVWGEYARI
                     LRGFLDHFPREQLLLLYSEDLRERGPESLRRVYRHLGISENRLPPNLGRLYHVGGARR
                     KVPGLEKILRSALARGLIRLAVSKRRYKRTQMKLERWNAKPGALAIPGETYDCLADHY
                     AGDIEELSRLSGVRPRWFEHRGESSVAL"
     gene            complement(9378..>9892)
                     /locus_tag="VFX38_07295"
     CDS             complement(9378..>9892)
                     /locus_tag="VFX38_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX5314689.1"
                     /translation="QTLERNRFVEIIMTRVTVPLGEDIGFLPGTEEEKMTPWMGALMD
                     NLEVLTTPEEGGAWARGATQDLLASRIKIRSLNFMRGRTFLNRYIIVDEAQNLTAKQL
                     RALVTRAGPGTKMVVLGNIAQIDTPYLSETTSGLTYVVDRFKDWPYAGHITLQRVERS
                     RLADYATEIL"
BASE COUNT         1769 a         3037 c         3237 g         1849 t
ORIGIN      
        1 gacgcgagcg atacggggca gggcatcttt cgcgtgcggg agacgattcc ggtgcagccg
       61 ggagagctga cgctgttgta tccgaaatgg attcccggca accacagtcc gagcgggccg
      121 atcgacaagc tggccggact cacgattacg gccggcggca agccgcttgc gtggaaacgc
      181 gacaaatacg acgtgtacgc gttccggatt gacgtgccga agggcgtggc gaaaattgat
      241 ctggattatc agttcctctc cgcgcgcggc gggaacgaag ggccgatcca gatgaccgac
      301 acgatgctgg acctggtgtg gagcaagctg gcgctgtatc cggccggaca ttacacgcgc
      361 gacatcagct atcgggcgag cgtgaagctg ccgcagggct ggcagttcgg gaccgcgcta
      421 acgaagcaat cgcaatcggg cgacaccgtg accttcgagc cgacgacttt caacaccctg
      481 gtggactcgc cgctgtacgc gggtctttat tacaagcggc tggatctcga tccgggttcg
      541 aaggtgccgg tgcacatgga catcttcgcc gacgcgccga agtatctcgt ggtcacgccg
      601 acgcagttga aaaagctccg cgcgctggta acgcaggcat acctgctgtt cgactcgcag
      661 cactacgatc actacgattt tctgttttcg ctctcggacc agttgagcgg caagggcctg
      721 gagcatcatc ggtccaccga ggtcgggacc atggccgact tcttcaccga ttgggacaag
      781 cgcgcgccgg gccgcgatct cctcgcgcac gaatacacgc attcctggaa cggcaagttc
      841 cgccgtccgg cggatctgtg gacgccgaat ttcaacgtgc cgatgggcga ttcgttgctg
      901 tgggtgtacg aggggcagac gcagtactgg ggctacgtgc tgaccgcgcg ttcgggcatg
      961 tggtcgcagc agcaatatcg cgacgcgctg gcgctggtcg cggcgaccta tgatcgcaac
     1021 cgtcccggtt tcgagtggcg cggcgtgcag gacaccacca acgatctcac catcgcgcag
     1081 cgtgcgccgc tgccgtaccg cagttggcaa atgagcgagg agtactacag cgccggacaa
     1141 ttgatctggc tcgcggcgga cgcgaaaatc cgcgcgctga cgcaggacaa gaagtcgctg
     1201 gacgatttcg cgagcgcatt ctttggcgtg gacaacggca gcttcgtgac caaaacctat
     1261 acgttcgacg atgtcgttgc ggccctgaac ggcgtggtga aatatgactg ggagaaattc
     1321 ctgcgctcgc gcctcgatgc gcatgcgccg ccgctcgacg ggatcgcggc atcgggctgg
     1381 aagctggtct atagcgacaa ggaaagcgac tatcagaagc agttgagcaa ggtgacgcat
     1441 cgtccgcaga atttcgcctt ctcgatcggc ctcgccctca acaaggacgg cggcatttac
     1501 gacgtgcgct ggaacggccc ggcgttcaag gccggcgttt ccgctggtgc gaccctggtc
     1561 gccgtcaacg gacacgcgta caagcccgag gtgctgaaag acgcgattac ggcagccaag
     1621 tcggacaagg cgccgatcga gctgttgctc aagtatcagg acgagtatcg gacggttccc
     1681 gtcgattatc acggcggcct gcaatatccg catctcgtgc gcatcaaggg cacgccggac
     1741 tatctcgatc agataatcac ggcaagaaag tagcggagtt cgaacctcga ggtcgtcgcg
     1801 gtttttccct gttctgtctg ctacaacacc atggcctgtg atcgtgagcc ttcgagaaag
     1861 ccagtgtgcg tttgcgcaca tacgcatcac gatgacggta cgcgatgcgc attgcgcgcc
     1921 ggtcgaagtc cgcaaatccg ctcatatcag cggcatttga aactgcacgt tgcgatttgt
     1981 tagcctgaga actcatcgac gagccgtgca cttttcaatg cacggcgaca ttcacggaat
     2041 cttagagagg tgagacagga tgaacaaatc aggcaacatt tttgcggtaa tggctggcgg
     2101 tgcgctcgcg gcgggcttgg ccctgtcgcc ggcgttcgcg ctcggcagca acaccgacaa
     2161 tttgcaggga gcgcgggcca agcagagtca aaaatacgaa gatctgactc aggcgaaggt
     2221 ccagaaagtc attgcgacat acaagaagga taccggcgcg ctggagaatt atttcaatga
     2281 cgcgtatgga tacgtggtat ttccgagcat cggtgtcggc agcaatgtgg ttggcaatga
     2341 tcacgggacc ggcgccgtct acagagacgg tgtactggtc ggacattcga ccgtcgtcaa
     2401 gttgcccatg aatgtggaaa ccggcgggca gaccttcagc gaaatgattt tctttcagga
     2461 caagcgggca ttcgagcttt tcacggaaga taaataccat ttccacggca agaccaccgc
     2521 tgccgacgtg cggggaggtt tgacgccgtc cacgcagtat tcgcacggga tggccgtatt
     2581 catcaatatg aagagtggtg ccgaaaccac tgcgcagaac gacaagatca agatcggcgc
     2641 gcaggagttc atgttcgacc cgataggcgc cgagcagaat ccgtccgcaa cgcatccctg
     2701 agggagcgcc cggcgaggcg tatccggctc gtgttcaggc cgggtgcgtt cgtgcctggc
     2761 tccaggttcc aggctgcaga aaatcgtgcg gcaccatcat cgtgacgaaa tgatgtcgcc
     2821 gctcatgggg acggctgggt tgcctgcagg ctcgcgagga aggtgttcag gatgtcgtag
     2881 aacttctgcg gctgatcgag catcgcgaag tggcgagacg gcgagatgga ttcgacctcg
     2941 acattcggga tgccggcgag aagcatctgg tagtacgcgg ttttctgtgc ctcgctgatc
     3001 ggaagcttgc tgtccttggc gaagtccggg ggattgtagg gcgaaatctc cagcaccgga
     3061 acgccgatgt tcttgagttg cgggcgcaga tcgagcgcca gatcctcgcc ggcatactcg
     3121 gccacggctg ccgggtcgct ttggtcctgc agcgccgcgt atttggccgc catcgccgga
     3181 tcgatcagtc cgatggtctg catgtaggcg agctgctgct cccggaattg ctccggtgac
     3241 agtgccgcca tcctggcgct cattcccctg ccgattgccg cgcgttgctc cgcgctcata
     3301 tcctgcgtgc cggggaacac ggggagcccg tccaccgcga ccaccccgct cagaagatcg
     3361 gcatgctcgc tcgcgaattg gagcgcgagg gtgccgccga gactgtggcc gacgagcacg
     3421 ggatgatcga tgttgtgcga gcggatgagt tcgagcagcg aggcatccgc ctgccccatg
     3481 agattttcgt gatttgcggg caagggcgtg ccatcgaagc ccgcaagcgt cagcacataa
     3541 atcacgtgat cgccctcgaa ctgcgcgacc gtaccgtccc agacccaggc gccgcttccg
     3601 agccccggaa tcagaatcag cgggcggccg tgatcgccgt aacgatgcac cgtgagcgtg
     3661 ccgaccgtga aggcggccgg accgctcggc gtcgtcgcgc aggcgcttgc caggagcgcc
     3721 gcggcgagaa tcgcgaacag gggacgaagg gtgctgcgca tggggaggct ccggtgctcg
     3781 cgaatcgcgc gagattgcga agcggccaag tatacgcccg gccgcgggcc tccacgcgga
     3841 atcggaagga cggcgcagcg ccggctaccg ctgcgcggta gctatttgcc ggatgtcgcc
     3901 ggtgcgccgt tcacgatccg gaatgcggtg ccgaccacct tcgaaagatc gcccatgtcg
     3961 gcgggcacga tcatcacgtt ggaggccttg gcgatgccgc cccactgcgc gatgaaatcc
     4021 tgcgcgacct gcatccgcat cgcctcgccc gcgccgtcct ggcgaagcgc cgcgccgacg
     4081 gtcgcgagcg ccttggcggt cgcgtcggca accgtcaata ctgcctgcgc ttcgccttcg
     4141 gcgcgcagta cctgcgcctg cttttctccc tccgcacgat tgatctgctg ctgacgttcg
     4201 ccctccgaca cgttgatcgc ctgctgcttt tcgccctccg actgggcgat gagcgcgcgc
     4261 ttctgccgct cggcagtgat ctgcagctcc atcgcgcgaa tcacctcttc cggcgggctg
     4321 atgtcgcgga tctcgtaacg cagcaccttc actccccagt tgacggcagc acggtcgagt
     4381 tcgttcacga tcgaggcgtt gagttcggtg cgcgaggaaa gcacctcgtc gagctgtttc
     4441 ttgccgacat ccgagcgcat gatcgtctgc gccaactgct ccaccgagga aagcgggttg
     4501 gaggtgccgt aggccgcggc cttgggatcg gtgatctgca gatacagcac gccgtcgatc
     4561 gaaatctggg tgttgtcctt ggtgatgcaa acctgctttc tcacgtcgat cggggtttcg
     4621 cggagatcga aacggtaggc gaccttgtcc gcgatcggaa tgatgaggtt gagtcccggt
     4681 tcgagaattc tctggaaccg cccgagccgc tcgatcaccc aggcatgctg ctgcggcacg
     4741 atgcgcaggc cgcggctgag gatcgtgagc gcgagaatca cgagaatgat ggcgacgacg
     4801 atgctgacgg tcatgggatt ctcctttgat gagtcgtctt tctcgaacga agatcaggcg
     4861 cgaccgtccg gcgcggacgc gagaacgagc acgttgccgt cgcgcgcgac gatgcgatag
     4921 ttgtcgccga tttgcggcgt cgcggcggca gcctggtcga ggcgggcatc ccagttgctg
     4981 ccgcgatagg cgacccggag cacgccgcca tcgctcgcga cgatcgtcac ccgattgccg
     5041 atgtcgtcgg ccgtgatttc gtcggacgcg cggtttttga gccgcccccg cgcccagtgc
     5101 gccaccggca ggccgaacag gatgacgccg gcgagcacga tcagggcgac gtccagtccg
     5161 ccgcctgcga acgcgacacc ggccgccgcg aagcaggcga cggcgacggc gatgaggtaa
     5221 atcgtgaggc tgtgaatttc ggcgacggtc gcgatcgcgc cgatcaccag aagcacgatt
     5281 cccagaacgg tttcggaacg catcgggttt tccctcgccg cggcgccgtg ggctccgctg
     5341 cggcgcattg tagcccagag ccgcgatggg ggcagccgac gtgccgcgac gccgctccgc
     5401 cggtcgggag cgccttcgcg gtcgcgccgg cgaccggcat tggcgcctgc gcttcgcctt
     5461 cggcgccgag agcggtcgcc tgcttctctt ctcgtactat tgatctggtg tcggcgctcg
     5521 ccgtcgaatg gaggccatct cccgaccgga atgacatccg atcccacgat ttgacaagtt
     5581 gaggtttgtc atgcaaacta gtctgcatag tgtcagaccc ggcacgaagt tcgcgcccgg
     5641 gcaagaggtc agatcaagtg aatgttgtga atgatgctgc tctggaacgt ctgcgcgagt
     5701 gggaagggct gacgcgcgat tatgcgcgtt tctcgcgcag tgccggtggc ctgggctccg
     5761 cactgggcgg ggtcctgacc ctcgtttcgt acttcgccgg cgctcttttg ccgtcggatt
     5821 cggccgcgcg cctgggtctg atcgccattc cgttcgtgtg gttgctcgtg cggggcgctc
     5881 tcgcgcgccg ctactaccag cgattcgggc atgtggagga gcaggagagt acggcgcagc
     5941 ggcggaccca tcgcttttgt gtcgccgtga ccttgctggt cgccgcggga atcacggccg
     6001 tgaccgcggg cgaagtgacg acaaagaccg gagcggacct cgccggcctg atcggctatc
     6061 tggcgctgat atggtttctg gttctcgcca cctggcgctg gttgcgcagc ccgctcgact
     6121 tcatcgtcgg cgtgtttctc ttctgtcagg ccgcgttgat ctgtgttggc cgatcgtatc
     6181 ctttgatctg gctgacgcat acgcgcgagg cgcttttgat gtcgctgatt gcggcgatgt
     6241 tcccgctcgc ggcgctggcg atgatcgccg ccggcatcgg cgatcatcgc cgcttcctgc
     6301 gcctgcgcac tcgtcttgcc gaattgcagc gaacggcggt gaaccgatcg tgagcggcga
     6361 atcggttccg gaaacgctgc tcggactgga ccgcctggtg cacgagccgg cgcggctgct
     6421 gatcttgagc gtgctcgcgg gcgccaagga agtcgagttc ggctttctcg aaaaaatgag
     6481 cggtctcagc aagggcaatc tttccagcca catgagcaag ctcgaggcgg cggggctgat
     6541 cggagtccgc aagcggtttc gggccaagcg accgctcacc gaattgcacc ttaccgcgaa
     6601 gggtcgcagg gccctggaag cgtaccgccg gcaattggca accgtgatgc gatccttgcc
     6661 cgccgaaccg gccgatggca aacgaagata aggccggaac cattcgaatg gaacgatcga
     6721 acaggaaatg aaacaggaga cgatgcgatg aagaactgct ttaccttcgt gccgggaatt
     6781 ctgtcggtcg cgttgacggc cctcgtgacg tcgctgttgc cgagcgcctg tgccgccgcg
     6841 gcgccgccgg ccgcggtctc ttcgctcctc ccggcgcgag tgctgcaagc ggcgcgggat
     6901 cgggtcgccg cgggcgagta tccggcgctc gtgatcggcg tggtggacgg cggcaggagc
     6961 cggctctatt ccttcgggaa actcgcgaac ggcgaggcgc cggatgcgaa gacggtgttc
     7021 gagatcggct cgatcaccaa gaccttcacg gccctgttgc tggccgaagc ggcgaaggag
     7081 gggagcgtcg cgctcgccga accggtcgcg aagctcctgc cgcacttctc cattccttcg
     7141 cgcgacgaca ggcagattac gctcgaggat ctcgccgacc agcactcggg cctgccgcgt
     7201 ctgccgacca atctcgatcc cgcgaatccc gccgatccct atgccggcta cgatgccgac
     7261 aggctcaagg catttctcgc gagttacgaa ctgccgcgcg atccgggcag cgcctacgaa
     7321 tactcgaatc tcggcttcgg cctcctcggc tacgcgctcg cgcggcacgc ggacatgacc
     7381 tacgatgcgc tggtcgagga gcggattctg caaccgctgg gcatgaagag gagcggagtc
     7441 gcgatgaccg ctgccatgcg cgcgcaattc gccgcgggcc acgacgcgac cggaaaatcg
     7501 gcagcgaact gggacttcgg cgtgcttgca ggcgcgggcg cgctcaagag tgacggcgcc
     7561 gacatgctgc gttacctcga agccaacatg ggcctgcgaa agagcttcct gtctgccgcg
     7621 atgcggctcg cgcagaaacc tctgcttgcg gcgaacggca aggaacgggt cggactcgcg
     7681 tggatgactc ttccgaccga ccgcggcgac gtcgtctggc acaacggcat gaccggcggc
     7741 tacgcgagtt tcatcggatt caccgccgac ggcaaacgcg gcgtcgtgat tctcaccaat
     7801 atccagcagt cggtggatga tctcggcttc gcgaccctgc tcgcggattg gccgctcgcg
     7861 ccggcggaaa aggcgattac tctgccggcg gacgaactcg acgactacgc cggcagctac
     7921 cggctcgcgc cgaaattcgt gctcacgatt tttctacagg gcgaccaact ctatgcgcag
     7981 gcgacggggc aggggccctt cccgattttc ccgagcgcga aagacgaatt tttcgcgcgg
     8041 gtgtcggaca tccgaatcag cttcgaacgc gacgcgcagg ggaaggtgac ggacctcgtg
     8101 ctgcatcaga acggcgaccg ccgggcgccg ctgatcggcg ccgcgcaggc cgcggccgcc
     8161 gcgacctcga cgccgattgt cgcgcttccc gcggcgacgc tgcgccaata cgtcgggcag
     8221 taccaactcg cgcccggcgc cgtcttcgac gtgacgctcg cgggcgatca actcagcgta
     8281 cggttgagcg gccagcccgc atttccggtc tatgccagcg ccaaagaccg attcttttac
     8341 acggtggtgg atgcgcgcat cgagtttgtc cgcgacgacg aggggaacgt ggttgccctc
     8401 gtgctgcatc agaacggcca ggcccgacgc gcgccgcgcg tgcaacaata gccgggcatg
     8461 gatccgcgct gctttttcgt catcggggcg cagaagggcg gaacgaccgc gctcttcgaa
     8521 tatcttcgtg gccatcccga agtgtacatg ccgccggaga aggaggcgga gtttttcacg
     8581 cgatgcgata cgacgccggc ggcctgggcc gagtatcgca agagacttct gactccgccg
     8641 cgccccgtgc gggtcgtcgg taccgcgagc ccgcagtaca tgggcgatcc tgcggtgccg
     8701 gcgcgcatgg cggccgtcgt tcccgacgcg cttttgatcg cggtccttcg cgatcccgtc
     8761 gagcgcgccg tctcgcacta caagatggcg tatcgccgcg gggaggagcg gcgcgacttc
     8821 gcgcgtgccg ttcgcgaaca attgcagccg gaggcgctcg agaaggcgcg ggcgctcccg
     8881 tttcgggaag tggcggagcg cgatgcctac gtggtctggg gcgaatacgc gcgcatcctg
     8941 cgcggctttc tcgatcattt cccgcgcgag cagttgttgc tgctctattc ggaggatttg
     9001 cgcgaacgcg gcccggaatc gttgcggcgc gtctatcgcc atctcggcat ttccgaaaac
     9061 cgcttgccgc ccaatctcgg gcgtctttat catgtcggcg gcgccagacg caaagttccg
     9121 gggctcgaaa aaattctgcg ttctgcactt gcccgcggcc tcatccgtct cgcggtgtcc
     9181 aaacgccggt acaagcgcac gcagatgaag ctcgaacgct ggaacgccaa acccggcgcg
     9241 ctcgcaatcc cgggtgaaac ctatgattgc ctcgccgacc attacgcggg cgacatcgaa
     9301 gaactcagcc gtttatccgg tgtgcgcccc cgctggttcg aacatcgcgg agaatcttcc
     9361 gtcgcgctct gaaggcgcta caaaatttcg gtcgcgtagt cggcgagacg cgaacgctcg
     9421 acccgttgca gcgtgatgtg tccggcgtag ggccagtcct tgaagcgatc cacgacatag
     9481 gtcaggcccg aggtggtttc ggacagatag ggcgtgtcga tttgcgcgat gtttccgagg
     9541 acgaccatct tggtgccggg gccggcgcgg gtgacgaggg cccgcaattg cttggcggtg
     9601 agattctggg cctcgtccac gatgatgtag cggttaagaa aggtgcgacc gcgcatgaaa
     9661 ttgagcgagc ggatcttgat ccggctcgcg agcaggtcct gggtcgcgcc gcgcgcccag
     9721 gcgccgcctt cctcgggcgt cgtgagcact tcgaggttgt ccatcagcgc gcccatccac
     9781 ggcgtcattt tttcttcctc ggtgccgggc aggaagccga tgtcctcacc cagcggcacc
     9841 gtgacgcggg tcatgatgat ctcgacgaag cgattgcgct cgagcgtctg tg
//
LOCUS       DASUJG010000336         2423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_95197, whole genome shotgun sequence.
ACCESSION   DASUJG010000336 DASUJG010000000
VERSION     DASUJG010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95197"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2423
                     /locus_tag="VFX38_07300"
     CDS             <1..>2423
                     /locus_tag="VFX38_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic beta 1-2 glucan synthetase"
                     /protein_id="HEX5314690.1"
                     /translation="YMAHHQGMTLLALDNALADGAMQRRFAADPSFQATMLLLQERIP
                     KPTGYFTHAAELTDLRMSAGGFESQVRVIGNPNTSVPEVQLLSNGRLHVMVTAAGGGY
                     SRWREFRVTRWREDTTGDGWGAFCYIRDTDSGVFWSAAFQPTRVRPAHYEAILSEARA
                     EFRRRDDAIETHTEITVSPEDDIELRRIRLVNHGRARRNLEVTSYAEVVLAVAGDDDQ
                     APAFSNLFVQTELLMERNAILCTRRPRSTGEQVPWMFHLMTAHGDAIEAGSCETDRMQ
                     FVGRGHTTAAPRALRDPGALSGSAGSVLDPIVAIRRYVTLAAGESAILDIVTGVSETR
                     EGCLRLIDKYQDPHLADRVFDLAWTHSRVVLGQLNATESDAQLYGRLAGSVLYANASL
                     RADHGTLRGNRRGQSGLWGYAISGDLPIVLLQVADVANIELVRQMVQAHAYWRIKGLA
                     VDLVIWNEDHSSYRQALHDSIMGLITAGVEAHALDRPGGIFVRRTEQIAEEDRILLQS
                     VARVIITDSKGTLADQINRRTPPTIVAPRVGQAPIGKIETEVDGALPDRALLFDNGIG
                     GFTPDGREYVITLGPGQTTPAPWVNVLANAQFGAVISESGQAYTWSENAHEFRLTPWH
                     NDPVSDTAGEAWYLRDEETGEYWSPTPLPRRGATSYVTRHGFGYSVFEHVERGIHSEL
                     WVYVAIDAAVKFAVLKTRNASGRTRHLSATGYVQWVLGDLPAKTGMHVMTEVDRRTGA
                     LLARNPYNTEFAGRIAFFDVDDPQRSVTGDRSEFVGRNGELGNPAAMDGSPLSGRVGP
                     AFDPCGVIR"
BASE COUNT          420 a          788 c          811 g          404 t
ORIGIN      
        1 ctacatggcg caccaccagg gaatgacgct gctcgcgctc gacaatgcct tggcggacgg
       61 cgcgatgcag cgacgcttcg ctgccgaccc gtcgttccag gcgacgatgc tgttgctcca
      121 ggagcgcatc ccgaagccga ccggctattt cacgcatgcc gccgagctga ccgatctgcg
      181 catgagcgcc ggcgggttcg aatcgcaggt gcgcgtgatc ggcaacccca atacctcagt
      241 gccggaagtg caactgcttt cgaacggcag gttgcatgtc atggtgaccg ccgcaggtgg
      301 cggctacagc cgctggaggg agttccgcgt cacgcgctgg cgggaagaca ccaccggcga
      361 cggatggggc gcgttctgct acatccgcga taccgacagc ggcgtcttct ggtcggcggc
      421 gtttcaaccg acgcgcgtcc gcccggccca ctacgaggcc attctgtccg aggcacgcgc
      481 tgaatttcgg cggcgcgacg atgcgatcga gacccatacc gagatcacgg tatcgcctga
      541 ggatgacatc gagttgcgcc gcatccgcct cgtcaatcat ggtcgggcgc ggcgcaatct
      601 cgaggtgacg agctacgccg aggtcgttct cgcggttgcc ggcgacgacg atcaggcgcc
      661 ggccttcagc aacctgttcg tgcagaccga gctgctgatg gagcgaaacg cgatcctctg
      721 cacgcggcgg ccgcggtcga ccggcgagca ggttccctgg atgtttcacc tgatgactgc
      781 ccacggtgac gcgatcgaag ccggctcgtg cgagaccgat cggatgcaat ttgtcgggcg
      841 cgggcatacc acggccgcgc ccagggcatt gcgcgacccc ggggcactct cgggtagcgc
      901 cgggtcggtc ctcgatccga tcgttgcgat caggcgatac gtgacgctcg ctgccggcga
      961 gtcggcgatc ctcgacatcg tcaccggcgt cagcgaaacc cgcgaaggct gtctccggct
     1021 gatcgacaag taccaggatc cccacctcgc cgaccgcgtg ttcgacctcg cctggacgca
     1081 cagccgggtg gtgctggggc agctcaacgc gaccgaatcc gacgcgcagc tctacggacg
     1141 gctggcgggg agtgtcctgt acgccaacgc ttcgttgcgt gccgaccacg gcactttgcg
     1201 cgggaaccgg cgcggccagt cgggcctctg gggctacgcg atttcggggg acctgccgat
     1261 cgtgctgctg caggttgcgg acgtggccaa catcgagctc gttcgccaga tggtgcaggc
     1321 gcacgcgtac tggcgcatca agggtcttgc ggtggacctg gtgatctgga acgaagacca
     1381 ttcgagctac cgccaggcgc tgcacgacag catcatggga ctgatcaccg ccggcgtcga
     1441 agcccatgcg ctggatcgcc ccggtggaat ctttgtccgc cgcaccgagc agattgctga
     1501 agaggaccgc attctgttgc agtcggtcgc ccgagtgatc atcaccgaca gcaaaggcac
     1561 gctcgccgac cagatcaacc gtcgcactcc accgacgatc gtggccccgc gcgtcgggca
     1621 ggcgccgatc ggcaagatcg agaccgaggt cgatggcgca ttgcccgacc gcgcgctgct
     1681 cttcgacaac gggatcggcg gcttcacgcc ggacggtcgg gaatacgtca tcacgctggg
     1741 tccgggacag accacacccg caccgtgggt caacgtcctc gcgaatgccc agttcggagc
     1801 cgtgatctcg gagagcggcc aggcgtatac gtggagcgag aatgcccacg agttccgtct
     1861 gacgccgtgg cacaacgacc cggtcagcga cactgccggc gaagcctggt atctgcgtga
     1921 cgaagagacc ggagagtact ggtcgccgac accgctgccg cgacggggcg cgacgtcgta
     1981 tgtcacgcga cacggattcg gctacagcgt attcgagcac gtcgagcgcg gcatccattc
     2041 ggaactctgg gtctatgtcg ccatcgatgc cgcggtgaaa ttcgccgtgc tcaagacgcg
     2101 caacgcttcc gggcgcacca gacatctctc cgcgaccggt tacgtccagt gggtactggg
     2161 cgatctcccg gcgaagaccg gcatgcacgt gatgaccgag gtcgatcggc ggaccggggc
     2221 cctgctcgca cgcaatccct acaacacgga gttcgcgggg cggatcgctt ttttcgatgt
     2281 cgacgatccg cagcgcagcg tgaccggtga tcgaagcgag ttcgtggggc gaaatggcga
     2341 gctcggaaat cctgccgcga tggatgggtc gccgctttcc ggccgcgtgg ggccggcgtt
     2401 cgacccctgc ggggtgattc ggg
//
LOCUS       DASUJG010000337         3248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1318359, whole genome shotgun sequence.
ACCESSION   DASUJG010000337 DASUJG010000000
VERSION     DASUJG010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318359"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VFX38_07305"
     CDS             <1..673
                     /locus_tag="VFX38_07305"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX5314691.1"
                     /translation="HVVEDAAHAIGTRDRGRLIGSFGELACFSFHANKNLTTIEGGAI
                     VTADERALTALRALRFHGQVKLDDGGYDVVSAASKYNMSDVCARIGLDQLAVLERRNA
                     RRRELAGLYFEALAELDELLLPARGDEGHAWHMFAVCVDFAKLGTDRKRFREALQAQG
                     IGTGVHYPALHGLSICREMSLRAEDFPNADRIGRETLTLPLFPEMSDGDVERVAASLR
                     SVMHG"
     gene            666..1622
                     /locus_tag="VFX38_07310"
     CDS             666..1622
                     /locus_tag="VFX38_07310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5314692.1"
                     /translation="MAETPRVSVVIPVYNEEAGLEALFARLYPALDALGVSYEVLFVD
                     DGSRDRSAAILRAQYQKRPEVTRVVLLQSNFGQHAAICAAFAKARGERAVTLDADLQN
                     PPEEIANLLAAMDAGHDYVGTIRRRRRDRWWRRSASRLMNRIREKITRVHMTDQGCML
                     RAYSRDVVDAINDTREIKTFIPALAYLYASNPVEIEVEHDERAAGASKYSLWRLMQLN
                     FDLVTGFSIAPLQVFALFGALVALGSLALFVWLMVDRIVHGPDVNGVFTLFAIAFFFI
                     GVLLFGIGILGEYIGRIYQEVQRRPRYLIRGTLGDDDKSGSE"
     gene            1619..2560
                     /locus_tag="VFX38_07315"
     CDS             1619..2560
                     /locus_tag="VFX38_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HEX5314693.1"
                     /translation="MSAAERARAVVFAYHDVGVRGLETLLARGVDVLLVVTHEDDPAE
                     AGWFASVADHARRYGIDTITPAPGDLPALGPRLAALAPDFLFSFYYRHLLPAALIETA
                     RGGAFNLHGSLLPRYRGRAPVNWAVLAGETETGASLHYMTARADAGDLVAQESVPILP
                     NDTAAEVMTKISVAGERLLWRVLPELIAGTAPRKPLDLAAGNYCGRRRPEDGRIDWSQ
                     GAWAIHNLVRAVAPPYPGAFSDIGGGRLFLLGSHFRNEAARHTRALLYVERGRMYADC
                     ADSFRFEVLKCAFNDNPVTAASFPFAGGEYPLSGDSL"
     gene            2557..>3248
                     /locus_tag="VFX38_07320"
     CDS             2557..>3248
                     /locus_tag="VFX38_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HEX5314694.1"
                     /translation="MKKVLILGVNGFIGHHLTRRILDSTDWQAFGMDMLNDRVAEWTD
                     HPRFSFSEGDITINREWVEYHVRKCDVVLPLVAIATPKTYVEDPLRVFALDFEANLPI
                     IRQCVKYRKRVIFPSTSEVYGMCTDKAFDPYESSLVLGPIPKERWIYSCSKQLLDRVI
                     WAHGGRDGLDFTLFRPFNWIGPGLDSIYTPKEGSSRVVTQFLGQIVRGDPISLVDGGK
                     RRRSYTYVSDGV"
BASE COUNT          541 a         1083 c         1012 g          612 t
ORIGIN      
        1 gcatgtcgtc gaggacgcgg cacacgccat cggcacgcgc gatcgcgggc gcctcatcgg
       61 gagcttcggc gaactcgcct gcttcagctt ccacgcgaac aagaatctca ccacgatcga
      121 gggcggcgcg atcgtgaccg ccgacgaacg tgcgctcacg gccttgcggg cgctgcgttt
      181 ccacgggcag gtgaagctcg acgacggcgg atacgacgtc gtctccgccg cgagcaagta
      241 caacatgagc gatgtctgcg cgcgcatcgg tctcgatcag ctcgccgtgc tggagcggcg
      301 aaacgcgcgc cggcgcgaac tcgccgggct ctatttcgag gcgctcgcgg aactcgacga
      361 acttctcctt ccggcacgcg gcgacgaggg gcacgcctgg cacatgttcg ccgtttgcgt
      421 cgactttgca aaactcggca ccgatcgcaa gcgtttccgc gaggccttgc aggcgcaggg
      481 catcggcacg ggcgtgcatt atccggctct ccacgggctc agcatctgcc gcgaaatgag
      541 tctgcgcgcc gaagattttc cgaacgcgga tcgcatcggg cgcgaaaccc tgacgcttcc
      601 gctctttccc gaaatgagcg atggcgacgt cgagcgggtc gccgcgtcgc tccgcagcgt
      661 gatgcatggc tgagacgccg cgggtctcgg tcgtcattcc cgtctacaac gaggaagcgg
      721 ggctcgaggc gctgttcgcg cgactttatc cggcgctcga tgcgctcggc gtttcttacg
      781 aggtgctctt cgtggacgac ggcagccgcg accgcagcgc cgcgattctg cgcgcgcaat
      841 atcagaagcg ccccgaggta acccgcgtcg ttctgctgca gtcgaacttc ggccagcatg
      901 ccgcgatctg cgccgccttc gccaaggccc gcggcgagcg cgccgtcacc ctcgatgccg
      961 atctccagaa cccgcccgag gaaatcgcga atcttctcgc ggcgatggac gccgggcacg
     1021 actacgtggg cacgatccgc cgccggcgtc gcgatcgctg gtggcgccgc tccgcctcgc
     1081 gcctcatgaa tcgcattcgc gaaaagatca cccgcgtgca catgaccgac cagggatgca
     1141 tgctgcgcgc ctactcccgc gacgtcgtgg acgccatcaa cgacacccgc gagatcaaga
     1201 ccttcattcc cgcgctcgcc tatctttacg cctccaatcc ggtggagatc gaggtcgagc
     1261 acgacgagcg cgcggcgggc gcatcgaaat attcgctgtg gcgcctgatg cagctcaact
     1321 tcgatctcgt caccggcttt tcgatcgcgc ccttgcaggt gtttgcgctt ttcggcgcgc
     1381 tcgttgcgct cggctcgctc gcgctgttcg tctggctgat ggtcgatcgc atcgtccacg
     1441 ggccggacgt gaacggggtg ttcacgctct tcgcgatcgc gttcttcttc atcggcgtgc
     1501 tgcttttcgg catcggcatt ctcggcgaat acatcgggcg tatctatcag gaagtgcagc
     1561 ggcggccgcg ctatctcatc cgcggaacgc ttggtgacga cgacaaaagc ggaagcgaat
     1621 gagcgcggct gagcgagccc gcgccgtcgt ctttgcttat cacgatgtcg gcgtgcgcgg
     1681 gctggaaacg ctgctcgcgc gcggggtaga cgttctcctc gtcgtcaccc acgaagacga
     1741 tcccgccgaa gccggctggt ttgcgagcgt tgcggatcac gcccgccgct acggcatcga
     1801 cacgatcacg ccggcgccgg gcgatttgcc ggcgctgggg ccccggctcg ccgcgctcgc
     1861 gccggacttc ctcttttcct tctattaccg ccatcttctg cccgctgctc tgatcgaaac
     1921 ggcgcggggc ggcgcgttca atctgcacgg atcgctcctg ccgcgttacc gcggacgtgc
     1981 gcccgtgaat tgggcggtgc tcgcgggcga gaccgaaacc ggcgcgagcc tgcattacat
     2041 gacggcgagg gccgacgcgg gcgatctcgt cgcgcaggaa tcggtgccga tcctgcccaa
     2101 cgatacggcc gcggaagtta tgacgaagat ctcggtagcc ggggaacgac tgttgtggcg
     2161 ggtgcttccg gaactgatcg cgggcacggc gccacgcaag ccgctcgatc tcgccgcggg
     2221 caactattgc ggacggcgcc gaccggaaga cgggcggatc gactggtcgc aaggagcatg
     2281 ggcgattcac aatctggtgc gcgccgttgc gccgccgtat ccgggcgcat tcagcgacat
     2341 tggcggcgga agactgttcc ttctcggcag ccattttcgc aacgaagccg cacgccatac
     2401 gcgagcattg ctgtatgtcg aacgcggccg gatgtacgcc gattgcgccg attccttccg
     2461 cttcgaagtg ctaaaatgcg cgttcaacga caaccccgtt accgccgcgt cctttccctt
     2521 cgcgggcggt gaatacccgc tttccggaga ctccctttga agaaagttct gattctcggc
     2581 gtgaacggct tcatcggcca tcacctgacg cggcgcattc tcgattcgac ggactggcag
     2641 gccttcggca tggacatgct gaacgaccgc gtcgccgagt ggaccgatca tccgcgcttc
     2701 agcttctccg aaggcgacat cacgatcaac cgcgaatggg tcgagtatca cgttcgcaaa
     2761 tgcgacgtgg tgctgccgct cgttgcgatc gcgactccca agacctacgt cgaggatccg
     2821 ctgcgcgtgt tcgccctcga cttcgaggcg aacctgccga tcatccggca atgcgtcaag
     2881 taccgcaagc gggtgatttt tccctcgact tccgaagtct acgggatgtg caccgacaag
     2941 gccttcgatc cctacgaatc ttcgctcgtg ctcggcccga ttccgaaaga acgctggatt
     3001 tacagttgct ccaagcaact gctcgatcgc gtcatctggg cacatggcgg ccgcgatgga
     3061 ctcgacttca cgcttttccg tccgttcaac tggatcggcc ccggtctcga cagcatctac
     3121 acgccgaagg aggggagttc ccgcgtcgtc acgcaattcc tcggccagat cgtgcgcggc
     3181 gatccgatca gtctcgtgga cggcggcaag cgccgccgct cctacaccta cgtcagcgac
     3241 ggcgtgga
//
LOCUS       DASUJG010000338         5665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1685396, whole genome shotgun sequence.
ACCESSION   DASUJG010000338 DASUJG010000000
VERSION     DASUJG010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1685396"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VFX38_07325"
     CDS             <1..274
                     /locus_tag="VFX38_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008207473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEX5314695.1"
                     /translation="VAKVTHIAPADPDGTIIGSPVVAPGVILYEVAKLGLCAGITAAR
                     YRTTTEVYPDSTRVTPEQCNAAQAAAACAAIEFALARASARENFDV"
     gene            267..461
                     /locus_tag="VFX38_07330"
     CDS             267..461
                     /locus_tag="VFX38_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HEX5314696.1"
                     /translation="MFETESVGIRCPYCGEAIEIVVDCSVAEQKYIEDCAVCCRPISI
                     AVTVAPDGSASVEASREDDA"
     gene            complement(513..1838)
                     /locus_tag="VFX38_07335"
     CDS             complement(513..1838)
                     /locus_tag="VFX38_07335"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX5314697.1"
                     /translation="MRSWKGLVFGAALLAAGTLLASVLTGLPAVVQKKSVTAFSKPDF
                     GAASVGTLHHGDAVRVANQDGLWYQIALANGATGFVRVNEIRIAQGSATGSADTMQIL
                     LTGKSGQGRVTETAGVRGLNESDLQSAAFDPVQLAKMQSYRATPESASAYAASHGWAS
                     TTIPWPGEAPPKGGSVSKSAVHSVTSAVGGLLGGVFGSAAKVADQATPESEAEQLQTE
                     LDLGPLIAGRVLGARPLWDNPQAQTRVNVIGRWLASQTSRPELPWTFGIIDDPEYNAF
                     AAPGGYILVTRGLYEIVANDEELASVLAHEITHVVQRDQYNVIRKQGLLSAGLQAASE
                     QVNTGGGVAENLARDYVEKNGAGILLTKLDRDAEYRADAIGEVYLARAGMNPLAMYAM
                     LQKMAATGTANGKLEELYATHPTIADRLNRIDERGYGALTPYLERPVPQ"
     gene            complement(1948..3768)
                     /locus_tag="VFX38_07340"
     CDS             complement(1948..3768)
                     /locus_tag="VFX38_07340"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEX5314698.1"
                     /translation="MLRNFLRRAMASIDAAMARLGLAFVAGWGHIEKGYRRPYARLTL
                     LLGFWFYPLFAVLALAWLAWDWSGPRNLAAAENAIFDQVISWRLTEPKPSGKVVIVEI
                     DDCSINYYRAKGEGGWPWSRARQADLLDALDRAGVRAVGFDIQFADPSASDPQSDSIL
                     EAMAEGGDGRFVFASTRLNQDYDSDSSLHVSQAPSAFPLVAHPDDDPTVALLLPYGKA
                     MQKNSALVNVTRNVDGVIRDVPLRERAGDWAIPSLALRLATGPDPAKMARYPASIRID
                     WRSRSRLPDVSAANLLAGKRICGNSAVAPPKLKGATVLIGYTAAGLNDAKPTPVSATM
                     PGVAIEAEAVEALLTRHWIWMPPPWFKYVIAALLVLLTGYAFFRGEPAWELDQIFVAG
                     NLVLLLVAYIGVTYFAVFLDIFAAVGFVALCFAVCRSYAHTQRGYAVGNDDYRQGFEP
                     ELHPWLAMVRLRFVPDPGIEARALDRRLREFRRRLRRFLYRGTDAVALDCVVEYDTWF
                     WDSMIDVTVLLWAGTDRAAVVARAERELDALHAYLAGMDSELPDDGSVRIACVITCAT
                     DERDSAATARTRVSAALGEVLREPIETPLATRNVFVSSGT"
     gene            4014..5054
                     /gene="xerD"
                     /locus_tag="VFX38_07345"
     CDS             4014..5054
                     /gene="xerD"
                     /locus_tag="VFX38_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEX5314699.1"
                     /translation="MVLFPGSLAARWSPWRRRPAACRRIGDSPTAANKTADPDARGAS
                     AAAGRESESAILIEGFLRALWLEQGLAENTRAAYRSDLRRLAEWCETRSLALMSVRSE
                     DLWQFLSVEAAAGVSARTGARRLAAIRHFYRHLAREGRVREDPSAELEAPRLPHSLPQ
                     TLSEQEVEALLQAPDVATDRGLRDRAMLELLYATGLRVSELVGLRLSELNLRAGMLRV
                     VGKGGRERIVPIGEEARHWIERYLARARPAIAGARRLDTVFPSPRRAALSRQAFWELI
                     GRYARAAGIRSHISPHRLRHAFATHLLEHGADLRAVQMLLGHQNVTTTQIYTHVARER
                     LRALHAEHHPRG"
     gene            complement(5172..5495)
                     /gene="fdxA"
                     /locus_tag="VFX38_07350"
     CDS             complement(5172..5495)
                     /gene="fdxA"
                     /locus_tag="VFX38_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005409054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HEX5314700.1"
                     /translation="MTFVVTDNCIKCKYTDCVEVCPVDCFHEGPNMLVIDPDECIDCT
                     LCETECPANAIFSEDDVPDDQRQYLALNAELSKDWPVLSEKIDAPADADDWNGKPDKL
                     PLLER"
BASE COUNT          939 a         1814 c         1961 g          951 t
ORIGIN      
        1 ggtggcgaag gtgacccaca tcgcgccggc cgatcccgac ggcacgatca tcggctcgcc
       61 ggtagtcgcg cccggcgtca tcctgtacga agtcgcgaaa ctcgggctct gcgccggcat
      121 cacggcggca cgctatcgca cgacgaccga agtctatccc gacagcacgc gagtcacgcc
      181 cgaacaatgc aatgccgcgc aggcggcggc cgcctgcgcg gcgatcgaat tcgcgctggc
      241 gcgagcgtct gctcgggaga acttcgatgt ttgagaccga gagcgtcggg atccgctgtc
      301 cctactgcgg cgaagcgatc gagatcgtcg tggattgctc ggttgccgag cagaaataca
      361 tcgaggattg cgccgtctgc tgccgcccga tcagtatcgc ggtgacggtg gcgccggatg
      421 gcagcgcatc ggtcgaagca tcccgcgagg acgacgcgta aggcctcacg acgaagctct
      481 tttcgggcgt gtcggcggcg ccgcggattt cgctactgcg gaacggggcg ctcgaggtaa
      541 ggagtgagag cgccgtagcc gcgttcatcg atgcgattca ggcgatcggc gatcgtcgga
      601 tgcgtcgcgt acagctcctc cagtttgccg ttcgccgtgc ccgtcgccgc catcttctgc
      661 agcatcgcgt acatcgcgag cggattcatg ccggcgcggg cgagatacac ttctccgatc
      721 gcatcggcgc gatattcggc atcgcgatcg agcttggtga gcaggattcc ggcgccattc
      781 ttctccacat aatcgcgggc gagattttcg gccacgccgc cgccggtatt gacttgctcg
      841 cttgccgctt gcaagccggc agacaggagc ccctgtttgc ggatgacgtt gtattgatcg
      901 cgctgcacga catgcgtgat ctcgtgggcg agcacgctcg cgagttcctc gtcgttggcg
      961 acgatttcgt agagaccgcg ggtgacgagg atgtagccgc cgggcgcggc gaaggcgttg
     1021 tattccggat catcgatgat gccgaaggtc cagggcagct ccgggcgcga agtctgcgag
     1081 gcgagccagc ggccgatcac gttgacccgg gtctgcgcct gcgggttgtc ccacaggggc
     1141 cgagcaccca gcacccgccc cgcgatcagc gggccgagat cgagttcggt ctgaagctgc
     1201 tcggcctccg actcgggagt ggcctgatcc gcgaccttgg ccgccgatcc gaagacgccg
     1261 ccgaggagcc cgccgacggc cgaggtgacg ctgtgcacgg cgctcttcga aacgctgccg
     1321 cctttcggtg gggcttcgcc gggccagggg atcgtggtgc tcgcccatcc gtgtgacgcg
     1381 gcatacgcgc tggccgattc gggggtggcg cgatacgact gcatcttcgc gagctgcacg
     1441 ggatcgaatg cggcgctttg caggtcgctt tcgttgagtc cgcgaacgcc ggcggtttcg
     1501 gtcacccggc cctgaccgga tttgccggtg aggagaatct gcatggtatc ggccgatccg
     1561 gttgcactcc cctgcgcgat gcggatttcg ttgacgcgca cgaagccggt ggcgccgttg
     1621 gcgagcgcga tctggtacca gaggccgtcc tgattcgcga cccgcaccgc atcgccgtga
     1681 tggagcgtgc cgacgctcgc cgcgccgaag tcgggcttgc tgaaggcggt gaccgacttc
     1741 ttctggacca ccgcgggaag cccggtgagg accgaggcga gaagagtccc ggcggccagc
     1801 agcgcggcgc cgaagacgag gcctttccaa ctgcgcatga tgatctcccg ggcgcgccga
     1861 cccttttcct gcgactccga tctgcctgca tgatacacgt ctgccggcgg gcagatcgct
     1921 cgtgatcccg ccgcccgcgg atgcggttca ggtaccggaa gaaacgaata cattgcgcgt
     1981 ggcgagcggc gtctcgatcg gctcgcgcaa cacctcgccc agcgccgcgc tgacgcgggt
     2041 tcgcgccgtt gccgcggagt cgcgctcgtc ggtggcgcag gtgatgacgc aggcgatgcg
     2101 cacgctgccg tcgtcgggaa gttccgaatc catgcccgcg aggtaggcgt gcagcgcatc
     2161 gagttcgcgt tcggcgcggg cgacgacggc ggcgcgatcg gtgccggccc acaggagcac
     2221 cgtcacgtcg atcatggaat cccagaacca ggtgtcgtat tcgacgacgc agtcgagcgc
     2281 cacggcgtcg gtgccgcggt agagaaatcg ccgcagccgg cggcggaatt cgcgcagtcg
     2341 gcgatcgagt gcgcgggcct cgatgccggg gtccggaacg aaacgcaggc gcaccatcgc
     2401 gagccaggga tggagttcgg gctcgaagcc ctgacggtag tcgtcgttgc cgacggcata
     2461 accgcgctgc gtatgcgcat acgagcgaca gacggcgaaa cacagggcga cgaagccgac
     2521 ggcggcaaag atgtcgagga acacggcgaa gtaggtcacg ccgatgtagg cgacgagcag
     2581 caacacgaga ttcccggcaa cgaagatctg gtccagttcc caggccggct cgccgcgaaa
     2641 gaaagcgtag ccggtgagga gcacgaggag cgccgcgatc acgtacttga accacggcgg
     2701 cggcatccag atccagtggc gggtgaggag cgcctccacg gcttcggcct cgatcgccac
     2761 tcccggcatg gtcgcgctca ccggcgtggg tttcgcgtcg ttgagaccgg cagcggtgta
     2821 gccgatcagc acggttgcgc ccttgagttt gggcggcgcc acggcggaat tcccgcagat
     2881 gcgcttgccc gcgagcagat tggcggcgct cacgtcgggc aggcgacttc ggctgcgcca
     2941 atcgatgcgg atgctcgccg gatagcgggc cattttggcc ggatcggggc cggtggcgag
     3001 acgcagcgca agcgagggta tggcccagtc gccggcccgc tcgcgcagcg gcacgtcgcg
     3061 gatcacgcca tccacgttgc gggtgacgtt gacgagggcg gagtttttct gcatcgcctt
     3121 gccgtaaggg agcaggagcg cgacggtcgg gtcgtcgtcg ggatgcgcga cgagcggaaa
     3181 cgccgagggc gcctgcgaga cgtgcagcga cgaatcactg tcgtaatcct ggttcagacg
     3241 ggtcgaggca aacacgaaac ggccgtcgcc gccctcggcc atcgcctcga ggatggaatc
     3301 ggactgcggg tcgctcgcgg agggatcggc gaactggatg tcgaaaccga cggcgcgcac
     3361 gccggcgcga tcgagcgcat cgaggagatc ggcctggcgt gcccggctcc acggccagcc
     3421 gccttcgccc ttcgcacggt agtaattgat cgagcagtcg tcgatctcga cgatcaccac
     3481 cttgcccgag ggcttgggct ccgtgaggcg ccaggagatc acctgatcga agatcgcgtt
     3541 ttcggcggcg gcgagatttc gcggcccgct ccagtcccag gcgagccagg cgagggcgag
     3601 gaccgcgaac agcggataaa accagaagcc aagcagcaga gtcagccggg cgtagggccg
     3661 ccgatagccc ttttcgatgt gcccccagcc ggcgacgaag gctagcccga gccgagccat
     3721 ggcggcgtcg atgcttgcca tcgctcggcg caggaagttt cgcaacattc accgctcccg
     3781 ggagaagcag cgcccatccc ggcactgcgg gagcggcgac ttcgcggccg atggtacgcg
     3841 tcgcgcccga catctggcaa gccgttcacg atccgccgtc atagcgctcg acgcggaagg
     3901 agagcgggac tccacggcgt cggagtgctt cccgtttgcg gccgatcctg ctatgttgcc
     3961 gtcgaggaag ggaattgcgg agcgccgaac gatgaaaggt tcaagcgaca ggcatggtcc
     4021 tgttcccggg ctctctcgct gcgagatggt cgccatggcg gcggcgtcct gccgcttgtc
     4081 gccgaatcgg agacagtccg acggccgcga acaagactgc tgatcccgac gctcgcggag
     4141 cgagcgccgc tgccggtcga gaatcggaat cggcgatcct gatcgagggt tttctgcgtg
     4201 cgctgtggct ggagcagggc cttgcggaaa acacccgcgc cgcgtatcgc tccgatctgc
     4261 ggcggctcgc cgagtggtgc gagacccgct cgctcgcgct gatgtcggtt cgcagcgagg
     4321 atctgtggca gtttctcagc gtcgaagccg ccgccggcgt gagtgcgcgc accggcgccc
     4381 gccgcctcgc cgcaatccgc catttttacc ggcatctcgc gcgcgaaggg cgcgtgcgcg
     4441 aagatccttc cgccgaactc gaagcgccgc gcctcccgca ttcgctcccg cagaccctga
     4501 gcgagcagga agtggaagcg ctgctgcagg cgcccgatgt cgcgaccgat cggggcttgc
     4561 gcgatcgtgc gatgctcgaa cttctctacg cgaccgggtt gcgggtgagc gaactggtgg
     4621 gcctgcgtct ctcggaactc aatctgcgcg cgggcatgct gcgggtcgtc ggcaagggcg
     4681 ggcgcgaacg catcgtgccg atcggagaag aagcgaggca ctggatcgag cgctatctcg
     4741 ctcgcgcgcg ccccgccatc gcgggagcgc gccggctcga cacggtcttt ccgagcccgc
     4801 gccgcgcggc actcagccgc caggcattct gggaactcat cggccgctac gcgcgcgcgg
     4861 cgggaatccg cagccatatt tcaccgcatc ggctgcgcca tgccttcgcg acgcatcttc
     4921 tggaacacgg cgccgatctg cgcgccgtgc agatgctgct tggccatcag aatgtcacga
     4981 cgacgcagat ttatacgcac gtcgcgcgcg aacgtctgcg tgctctgcat gcggagcatc
     5041 atccgcgcgg ctgactcgcg gtgctcgccg cacgtcgacg gcgtcgccgc ctgtcaggaa
     5101 ttcagtgatc cgcgttccgg ctgactcctg actcacttac aggccgtgca gcggccgact
     5161 tgcttcctcg atcagcgctc gagaagcggg agcttgtccg gtttgccgtt ccagtcgtcg
     5221 gcgtccgcgg gcgcatcgat cttttcggaa agcaccggcc agtccttgga aagttcggcg
     5281 ttcagtgcca gatactggcg ctgatcgtcg ggcacgtcgt cttcggagaa aatcgcgttg
     5341 gccgggcact cggtctcgca aagcgtgcaa tcgatgcact cgtcgggatc gatgacgagc
     5401 atgttggggc cctcgtggaa acagtccacg gggcagacct ccacgcaatc ggtgtacttg
     5461 cacttgatgc aattgtcggt gacgacgaac gtcatctcgc acgcctcggc tcgaaagcgc
     5521 gactattttg ccacagcagg atgctggttc tccttgatgc agctcaatgg tcgcgttcga
     5581 cgatgagccg ggcgatcgtg acgagcggtt cggcggcgtc gccgaagggt tccagcgcct
     5641 cgagggcctc ggaacaggcc gcggc
//
LOCUS       DASUJG010000339         3208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1564210, whole genome shotgun sequence.
ACCESSION   DASUJG010000339 DASUJG010000000
VERSION     DASUJG010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1564210"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..138)
                     /locus_tag="VFX38_07355"
     CDS             complement(<1..138)
                     /locus_tag="VFX38_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX5314701.1"
                     /translation="MRKLKVAAVQMHMSEEREDNLARAEGFVRAAAGRGAKLVLLPEL
                     FA"
     gene            complement(126..1184)
                     /locus_tag="VFX38_07360"
     CDS             complement(126..1184)
                     /locus_tag="VFX38_07360"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEX5314702.1"
                     /translation="MAVSRTGPTPAATAGVALRGEWRRHRRTWMAWPWHPETFVGTVA
                     AAQEAYARVANTIGEFEPVAMVVRPGHQRKARRLLSAAVEILVWPIDDAWMRDIAPSF
                     VVDGSGRMAGVDWEFNDYGNKDERGLEGYGNDAATAERILAHLGIKRRAAPLVCEGGT
                     LVADGEGTLITTESVLLNRNRNPELGAEAIEAIFAEYLGVRRTIWIPEGLDHDDTDGH
                     ADNLVAFAAPGVVLALAEDDPGDLNYTVLADLRRRLAAARDARGRRLEILSLPQPRAR
                     YKRRMRQALSYVNFCLVNGGVLIPAYDDPRRDANARSIVAEAFPGRKAVSLPTLAIAD
                     GGGSLHCITHEEPDPCAS"
     gene            1440..>3208
                     /locus_tag="VFX38_07365"
     CDS             1440..>3208
                     /locus_tag="VFX38_07365"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX5314703.1"
                     /translation="MTRQASLPVAAGVSAILFLFLCASPLAMAADAATAQAAPLPTIE
                     VTGTRLSEPSLAAANPVVTITRAQIEASGFTQLGELINSLPFTGAGQNTHWNANGTGR
                     VLADIHNLGSNRVLVLVNGQRWVTTNSGPVDLSTIPLEVVDHVEVLLDGASAIYGSDA
                     ISGVINIITVKNFVGAAAATQIGMYDAHGDGGGWDGKMYGAHFLLGTGNERSNVTLGA
                     GYYNSNPVFAGNRDISRYPVAGFGNLLGSPITPDGHLILDTNDPGPFGSDCSAGGEGF
                     AYHCDLAGPLDGDSAHPFTTADLYNDAADTYIQTPQERWYLYGQGHYDFTDNVEFVFM
                     TTYERRNATELTPPGGWDIGSQGSIKVNGLPVGIAGSNPYNPFGMDLVPAAPGTPTFD
                     AWCARWGSAADGGCTDDFGMLDYLGIRPLGQGSETITQNLDNYYFNGGFDGSFSAFGN
                     PWTWNANYAYGQSLETEIRHGLTLAEPLQVGLGPADVCDGSPGCVPLDFFGGSAGVTP
                     AMIGYTNFVAHFVSDFISRDYNANISGGFFNGWYAGPWQVAAGYEYLNENGEFEPDAT
                     VIKGLWTGFAIQPQYGRKNSNAQ"
BASE COUNT          542 a         1035 c         1067 g          564 t
ORIGIN      
        1 cgcgaagagt tccggaagaa gaacgagttt tgcgccgcgg ccggccgcgg cgcggacaaa
       61 gccttcggcg cgggcgaggt tgtcctcgcg ctcttcgctc atgtgcatct gcaccgcggc
      121 gaccttcagc ttgcgcatgg atccggctcc tcgtgggtaa tgcagtgcag gcttccgccg
      181 ccgtcggcga tcgcgagagt cggcagcgag accgccttgc ggccggggaa agcctcggca
      241 acgatgctgc gcgcgttcgc gtcgcggcgc ggatcgtcgt aggccggaat caggacgccg
      301 ccgttgacga ggcagaaatt cacataggaa agagcctggc gcatgcgccg cttgtagcgc
      361 gcccgcggct gcggcagaga caggatctcg aggcggcggc cgcgggcgtc gcgcgccgcg
      421 gcgagtcggc ggcggagatc ggcgagtacc gtgtagttga gatcgcccgg atcgtcttcg
      481 gcgagggcga ggacgacgcc gggcgcggcg aaggcgacaa ggttgtcggc gtggccgtcg
      541 gtgtcgtcat ggtccaggcc ctcgggaatc cagatcgtgc ggcgcacgcc gagatactcg
      601 gcaaagatcg cctcgatcgc ctcggcaccg agttcggggt tgcggttgcg gttgaggagc
      661 acgctttcgg tggtaatgag cgtaccctcg ccgtcggcga ccagggtgcc gccctcgcag
      721 acgagcggcg cggcacggcg cttgatgccg agatgcgcga ggatgcgctc ggccgtcgcg
      781 gcgtcgttgc cgtagccttc aagaccgcgc tcgtccttgt tgccatagtc gttgaattcc
      841 cagtccactc cggccatgcg cccgctgccg tccaccacga aacttggcgc gatatctcgc
      901 atccaggcat cgtcgatcgg ccacacgaga atttcgaccg cggcggaaag cagccggcgc
      961 gctttgcgct ggtgcccggg gcgcaccacc atcgccaccg gttcgaactc gccgatggtg
     1021 ttggcgaccc gggcgtaggc ctcctgggcc gcggcgaccg tgccgacgaa ggtttccgga
     1081 tgccagggcc aggccatcca cgtgcgccgg tgccggcgcc attcgccccg gagcgcgact
     1141 cccgcggttg cggcgggagt ggggccggtt cgggaaacag ccatgagaat tcggaagggc
     1201 ggacggacgg catcagtata aggaaagggt gggcaattca cggtttgcgg gcggaaccgg
     1261 gacgtcggtc ggcgccggat cgcgcgcaaa agatcgattc cgtggtgggc ggatcgaagg
     1321 tcgcgcgatt ggcatgacaa gatgctaatg aaatatttgt aatctttcgg aatttctgct
     1381 agcgtgcgct tcgccaaccg gggtttttct tcggtttcca accaagggga tttgctctca
     1441 tgacgcgtca agcttcgctg ccggtcgcag ccggcgtatc cgcgattctg ttccttttcc
     1501 tctgtgcttc gccactggcg atggcggccg acgctgcgac ggctcaggcg gcgccgctgc
     1561 cgacgatcga ggtgacgggc acccgcctca gcgagccttc gctcgccgcc gccaatccgg
     1621 tcgtcacgat cacccgcgcg cagatcgagg cgagcgggtt cacgcagctc ggcgagttga
     1681 tcaactcgct gcccttcacc ggcgccgggc agaacacgca ctggaatgcc aatggcaccg
     1741 ggcgggtgct cgcggacatc cacaatctcg gctcgaaccg ggtgctggtg ctggtcaacg
     1801 gccagcgctg ggtcacgacc aacagcggcc ccgtggatct ttccaccatt ccgctggaag
     1861 tcgtggatca tgtggaggtg ctgctcgacg gtgcctcggc gatctacggc tcggacgcga
     1921 tatcgggcgt catcaacatc atcacggtga agaacttcgt gggcgcggcg gccgcgaccc
     1981 aaatcggcat gtacgatgcg cacggcgatg gcggcggctg ggacggcaag atgtatgggg
     2041 cgcattttct gctcggcacc ggcaacgagc gctccaacgt cacgctgggc gccggttatt
     2101 acaactcgaa ccccgtcttc gccggcaatc gcgatatttc gcggtatccg gtcgcgggct
     2161 tcggcaatct tctcgggagc ccgatcaccc ccgacggaca tctgattctc gacaccaacg
     2221 atcccggacc cttcggcagc gattgcagcg ccggcggcga aggcttcgcc tatcactgcg
     2281 acctcgccgg accgctcgac ggtgacagcg cgcatccctt cacgacggcg gatctctaca
     2341 acgatgcggc cgatacctac atccagacgc cgcaggagcg ctggtacctc tacgggcagg
     2401 gtcattacga tttcaccgac aacgtcgaat tcgttttcat gacgacctac gagcggcgca
     2461 acgccaccga actcacgccg ccgggaggct gggacatcgg ttcgcagggc agcatcaagg
     2521 tcaacggtct gccggtcgga attgcgggca gcaatcccta caacccgttc ggcatggatc
     2581 tcgtcccggc cgctcccgga acgcccacct tcgatgcctg gtgcgcgcgt tggggcagcg
     2641 cggccgacgg cggctgcacc gacgatttcg gcatgctgga ctatctcggc atccgcccgc
     2701 tcgggcaggg atcggagacg atcacccaga acctcgacaa ctactacttc aacggcggtt
     2761 tcgacggttc gttttcggcc ttcggcaatc cctggacctg gaacgccaac tacgcctacg
     2821 ggcagtcgct cgaaaccgaa attcgccacg ggctgacgct cgccgagccg ctgcaggtcg
     2881 ggctcggtcc cgcggatgtc tgcgacggct cgccgggctg tgtgccgctc gatttcttcg
     2941 gcggttccgc cggggtcacg ccggcgatga tcggctacac caatttcgtc gcgcatttcg
     3001 tgagcgactt catcagccgc gactacaacg cgaacatttc gggcggcttc ttcaacggct
     3061 ggtacgccgg tccctggcag gtcgcggcgg gctacgaata tctcaacgag aacggcgaat
     3121 tcgagcccga cgcgacggtc atcaagggac tgtggacggg cttcgccatt cagccgcagt
     3181 acgggcgcaa gaattccaac gcccagta
//
LOCUS       DASUJG010000340         8400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_954047, whole genome shotgun sequence.
ACCESSION   DASUJG010000340 DASUJG010000000
VERSION     DASUJG010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8400
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954047"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="VFX38_07370"
     CDS             complement(<1..800)
                     /locus_tag="VFX38_07370"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX5314704.1"
                     /translation="MALKPRTQAWLLAGALCAIAPWVALAALADSSGAPNPVQTDPDD
                     SDAPASDPPRRLLAAKPGTKSEAAQLPSISVTAKAPAQSYRVYETSTATGTDTPILDI
                     PQSIQAIPDSVLRDQAAQSLADAVRNAPGIYVQQGEGNRDEFFLRGVKTKSDFFEDGL
                     RDDSLYYRDLYNIEQVDVLQGPAAILFGRGGAGGVINLVTKKPERRPIRNFGFELGSW
                     EHYRGTLDVGGAVGESGAFRFMAMGEDSGGFRDHFFLHRYAANPTFSF"
     gene            1213..1833
                     /locus_tag="VFX38_07375"
     CDS             1213..1833
                     /locus_tag="VFX38_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314705.1"
                     /translation="MARPAADTTAPPPATATYFAKVIAPQSLTASQTGEADVYVGTSA
                     YIKKRASVPSAATASKTVELARGLKQSAEVTLAGGAFEIAPIVPSRGKPNCYALGEYG
                     SQFPFAITPRKNATSGSYQINAHVLLYPQRDCVGTPSSQPSEPATVTVHVKSGARKNW
                     NQLAAITWTEFKKFWGALVALVFALVLFLIRKRLAKWFGFKSKGDE"
     gene            complement(2106..3230)
                     /locus_tag="VFX38_07380"
     CDS             complement(2106..3230)
                     /locus_tag="VFX38_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314706.1"
                     /translation="MNTALVVDIAEFAGLFTISALVRSRFRHHGYSATAAGGIVMAIG
                     IFADAALRELPFYSPTLARLITLEALVIWLFLAWSYLLAATNRHFRMHMQHPLRRFAI
                     GTWVAGTAVLSVICVHYLPGAPLLGKTLALVAVVVYLPYVVLFVNGYLRLWRHPAKQN
                     ANGVILLATVSTQSVVIALDAAFGGGFATNIAIGMVAFDMIFLCSGLVLVAIHYHAVG
                     SWFLAIEWKNANCILHGAISITGLALVLTSNFNADVLFGVWEVALVLFVAVESIEFLR
                     MLERERRRGLRLGLLVYDSTQWTRNFTYGMFYAFSLALYHRTDFLQTATGAFWWPALD
                     PIVRWGQYAVLAILLVEILLFLRARLHLFWRVGARITREV"
     gene            complement(3348..4567)
                     /gene="ampC"
                     /locus_tag="VFX38_07385"
                     /pseudo
     CDS             complement(3348..4567)
                     /gene="ampC"
                     /locus_tag="VFX38_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008348358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class C beta-lactamase"
     gene            complement(4601..6475)
                     /locus_tag="VFX38_07390"
     CDS             complement(4601..6475)
                     /locus_tag="VFX38_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX5314707.1"
                     /translation="MNNPDSAIAVARPRVPRRLGDFNVNPRILLLSGLALVVGAAAAL
                     VADALLKLIGLITNLVFYQRVALSLVAPSGGHDQWWLIIAAPVVGGLIVGFMARYGAE
                     AIRGHGMPEAIEAILRGGSKIRPRIAVLKPISAAITIGTGGPFGAEGPIIMTGGAFGS
                     LLAQTLSLTADERKTLLVAGAAAGMAATFNAPLASILLAVELLLFEMRPRSFVPVTIA
                     VVVATALRWPLLGFAPIFATSHALPTVSPIVLCLCVVAGLVGGFVAWLATLLVYLSED
                     AFRKLPIHWMWWPAIGGLIIGVGGLFEPRALGVGYDVIRELLAGQATLALIVGILVVK
                     TLIWSLSLGSGTSGGVLAPVFMIGAALGAVLGYVFPHVAPGFWSLMCLAAVVGGVMRS
                     PLTGVIFAMELTHQWDALLPLVVASTAAFLLSTLVLKRSVLTEKVVRKGTHVTREYSV
                     DPLDVFLVGEIMPRNFVSFRSGTSLREAATEAAGAWSGGGKRKLEHGQRLYPVLDGDD
                     RMIGLITRHQLLDAAFELEGAPDTRLDDVMIREMVLGYPDMTLRELANLMAEREIANL
                     PIVARDDPRQVLSVMTIEHVLQGRLVDLNEERKTERVLLVPLIRAVRGGLIVMRRRRG
                     "
     gene            complement(6536..7471)
                     /locus_tag="VFX38_07395"
     CDS             complement(6536..7471)
                     /locus_tag="VFX38_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012983432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEX5314708.1"
                     /translation="MSAVISGSLAYDVIMRFPGRFGDHILPERIHKLSVAFMVSELRR
                     EFGGCAGNIAYTLTLLGERALPLGAAGRDFAPYAKWLDQNGIARESILELGDVYTAQA
                     FINTDNDGNQITAFYPGAMARAHEQTLPRIADARIGIVAPDGRDAMIRRAEEFAAAGI
                     PFLFDPGQGLPMFDGSDLEYFLQRASWLAMNDYEAGVFCEKTRLGLDEIADKLKAVIV
                     TRGAHGSVVHAEGNQYDIPAVEPNAVVDPTGCGDAYRAGLLYGLLHDLEWETTGRIAS
                     LAGSIKIASEGTQNHRFTPAEFAARFEAAFGYSFH"
     gene            complement(7468..>8400)
                     /gene="gcvPB"
                     /locus_tag="VFX38_07400"
     CDS             complement(7468..>8400)
                     /gene="gcvPB"
                     /locus_tag="VFX38_07400"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX5314709.1"
                     /translation="YTVREIPTAGDGDVNLEALAAAVGEKTAGIMLTNPSTLGVFERR
                     IGEIAKIVHAAGGLLYYDGANMNAILGKVRPGDMGFDVMHFNLHKTFSTPHGGGGPGA
                     GSVAVGPRLEPFLPLPLVAKAGERYRLLDEKDRPQSIGRLSAFNGNVGVLLRAYVYIR
                     LLGADGMQRVAEYATLNAAYLAARLVAAGFELAYPGRRASHEFIVTLKKLAKTQGITA
                     MDFAKRLLDYGVHAPTTYFPLLVPECLLIEPTETESKETLDAFVDAMAAILAEAQDEP
                     EKLKGAPYTTPVGRLDDVKAARELDLAWQGDLGS"
BASE COUNT         1569 a         2637 c         2844 g         1350 t
ORIGIN      
        1 tcaaagctga acgtgggatt cgccgcgtag cgatgcagaa aaaaatgatc gcgaaagccg
       61 ccggaatcct cgcccatcgc catgaagcga aacgcgccgc tctcgccgac ggccccgccg
      121 acatcgagcg tcccgcgata atgctcccag gagccaagct cgaagccgaa attgcggatc
      181 ggccggcgtt cgggcttctt cgtgaccaga ttgatgacgc cgcccgcgcc tccgcggccg
      241 aacaggatcg ccgccggccc ctgcaatacg tcgacctgct cgatgttgta gagatcgcgg
      301 tagtacaggc tgtcgtcgcg caggccgtcc tcgaagaagt ccgatttggt cttgactccg
      361 cgcagaaaga actcgtcgcg gttgccctcg ccctgctgca cgtagatgcc gggcgcgttg
      421 cgcaccgcat ccgcgagcga ctgcgccgcc tgatcccgca gcaccgagtc cgggatggcc
      481 tggatcgact gtggaatgtc gaggatcggg gtgtcggtgc cggttgccgt gctggtttca
      541 tagacgcggt agctttgcgc cggcgctttg gcggtcacgg aaatcgacgg caactgggcg
      601 gcttccgact tcgtgcccgg tttcgccgcg agcagccgtc gcggaggatc ggaggcgggt
      661 gcgtcggaat cgtccggatc ggtctgcacc ggattcggcg caccggacga gtccgccagc
      721 gctgcgaggg cgacccacgg ggcaatcgcg caaagcgccc ccgcgagcag ccaggcttgc
      781 gttcgtggct tcagggccat gaatgttcct tggagatgga taggagcggg gaatattaac
      841 aaattgtaat ctctggcccc tgatcccgct gggccggcgc tgcggggatc cgccgcccgc
      901 ctgccatgcc cgggcttttt cgcggctccg ccgccgcgac actcgtcggc aaagcccttg
      961 caagggtgcc aaaggaagcg tctaatacgc cgtcacaact tgtcgcggca ggcgggtatg
     1021 gagcagcggg gacgatccgg ctcgtcgggg aagctcgccg ccaatcgaag ctggaggtta
     1081 agggcgcaga atcgcgggac cctgctcgcg atgttcgttg gctttccgct gatgatcggg
     1141 cttggcgcct gcggcaaatc gtcggccccg ccaccgcccc cgcctccccc tccgccgcct
     1201 ccgccgcaag cgatggcgcg gccggctgcg gacacgaccg ccccgccgcc ggcgacggcc
     1261 acgtattttg ccaaggtcat cgcaccgcaa agtctgacgg cgagccagac cggcgaggcg
     1321 gacgtctacg tcggcacctc ggcctacatc aaaaaaagag cctcggtacc gtcggcggcg
     1381 accgcttcga agaccgtcga gcttgcgcgc gggctcaagc aatccgccga agtcacactt
     1441 gccgggggcg cattcgagat cgcgcccatc gttccgtcgc gaggcaagcc gaattgctat
     1501 gccctcggag aatacggatc gcagtttccc tttgccatca ctcccaggaa aaatgcgacg
     1561 agcggctcct atcagatcaa tgcgcatgtt ctgctgtatc cgcaacgcga ctgtgtcggc
     1621 acgccctcct cgcagccgtc ggaaccggcc acggtaacgg tgcacgtcaa gagcggcgcg
     1681 cgcaagaatt ggaatcagct cgccgcgatt acctggaccg aattcaagaa attctggggt
     1741 gccctggtcg cgctcgtctt cgcgctggtc ctgttcctga tccgcaagag gctcgcgaag
     1801 tggttcgggt tcaagagcaa aggcgacgaa tgaaccgcgt cctcgccggc gaccccgcac
     1861 aggatcgccg ccgtcggaac gacgccgcag gagcccaagg cccagggcaa cattgatcaa
     1921 ctttggcgtc gtcattccgg aatcgcgaag cggtgtccgg aatccagtac ggggaacgta
     1981 tcccgcccgg agggcgggat tcatgatcgt gactggatcc cggccttcgc cgggatgacg
     2041 accgagcaat ccccgaccag cgcagcacgg cgggacttct ccgcatcccc gtggcgatac
     2101 gcgactcaga cttcgcgggt gatgcgcgcg ccgacacgcc agaaaagatg caggcgggcg
     2161 cggagaaaga gcagaatctc gacgagcaga atggcgagta cggcgtactg accccagcgc
     2221 acgatcggat cgagcgccgg ccaccagaat gcacccgtcg ccgtctgcag gaagtcggtg
     2281 cggtggtaga gagcgagcga gaaggcgtag aacattccgt aggtgaagtt ccgcgtccat
     2341 tgcgtggagt cgtagacgag aagtccgagg cgcaggccgc ggcgacgttc acgttccagc
     2401 atccgcagga actcgatgga ctccaccgcc acgaacagca ccagcgccac ttcccacacg
     2461 ccgaagagca cgtcggcatt gaagttcgag gtgagcacaa gcgcgaggcc ggtgatcgaa
     2521 attgctccgt gcaggatgca attggcgttc ttccattcga tcgcaaggaa ccagcttccg
     2581 acggcgtggt aatggatcgc gacgagaacg aggccgctgc agagaaaaat catgtcgaag
     2641 gcaaccatgc cgatcgcgat gttggtcgcg aatcccccgc cgaaggccgc gtcgagcgcg
     2701 atcacgaccg attgggtgga gacggtcgcg aggaggatga cgccgttcgc gttctgtttc
     2761 gcgggatggc gccagagacg cagatacccg ttgacgaaca gcacgacata gggcagatac
     2821 acgacgacgg cgacgagcgc gagcgttttc ccgagcagcg gagccccggg aaggtagtga
     2881 acgcagatca cggaaagcac tgcggtcccc gcgacccagg ttccgatcgc gaagcgccgc
     2941 agcggatgct gcatgtgcat gcgaaagtgg cggttggtcg cggcgagcag gtaggaccag
     3001 gcgagaaaca gccagatcac cagagcttcg agggtgatga gccgggcgag cgtcggcgag
     3061 tagaagggca actcgcgaag cgcggcatcg gcgaagatgc cgatggccat gacaatgccg
     3121 cccgctgcgg tagccgaata gccgtgatgg cgaaagcgcg agcgcaccag ggccgagatc
     3181 gtgaagaggc cggcgaattc cgcgatgtca acgacgagcg cggtattcat cgaatcccga
     3241 tggctggaac gcttcgacgc gatacggaat gggcatcacc ttcttattgt gccgccccgt
     3301 gccgtcgcgg cgcaaagcgg tgcatttccc cgtcgcggtg ctgcggctca gtttcgccgg
     3361 ccggatgccg cgctttccgg ctccggcgcc gatgtccgtt ccagaccggc aactcttccg
     3421 agaagttcgc gcgccagctt cacgcgatcc gggatcggaa ggctcttgtt ggcgaggatg
     3481 acgatgcccg cacgcttctg cgggataaag gcaacgtagg ccccgaaccc gaaggtcgag
     3541 cccgtcttgt tgatccagac gtcggggcgt ggcggctcgg ccggattcag cttgaccgcg
     3601 ggattcggct ccgaaatcat ttggtagccg tttccccgca gagcagatcg gcgagtgcca
     3661 cgggaagcgg gtattgctcc catacgaggt cctgcgtcat cggacccacg cggaagtagc
     3721 cggtgtgcgt ggttctgatc gccgtggcga gcgcgggatc ggatatcggc gcccccattt
     3781 gcgccttgag aaagcgaagc atttccgcgg cggtcgatcg gatcccgtag gtttcttccc
     3841 acaaaatgct cttcgacatc cggatcgggg catcatccga gtcatatcct tgcgcatagt
     3901 cctgctgccg atccgcggga acctggagga aggtgctcgt catgcccagg ggacggagaa
     3961 tgcgcctctc gacgagcgtg gcgaaatcgg cgcccatgct tttggcggcg atcatgccga
     4021 gcgtgccggc gctgggattg ctgtacgtcc ggtacgttcc ctgcgcgtgc gtcgggcgcc
     4081 agcggaggta gtagtccatc agttgggcct ggtcgtggat gccgtcgggg aactgcagcg
     4141 gcaggatgcc cggagtgtag gttccgagac tgagcaggcg caacttcccg aaggggcttc
     4201 cacgcaattg cggaagatgc gtctcgaccc gatcgctcaa ggaaagccgg ccttcccctc
     4261 gggccagctc cgtcaaggtt gccgtgaacg tcttggtcac cgaaccgagt tcgaacagcg
     4321 ttgtggaatc gacgggacgc cgctctttct tcgaggccag tccgtagtag aggagccagt
     4381 catgaccggc aaccgtgacg ccaatggcca tgcccgcgat gcggtccttc gccatcaagg
     4441 tctgcactgc ctcggccgcg aacgtctgga aacccgctga tttctgcgct tcggcggcag
     4501 ccgatgccgg gctccctgcg agtgcgccga ctgccagcga cagcagggtc aagccggtaa
     4561 tccgcagaga aatcactccg aaaactccgt gcacgagact tcagccgcga cgccgccgca
     4621 tcacgatcag gccgccgcgc acggcgcgga tgagcgggac gagaagcacg cgctcggtct
     4681 tgcgctcctc gttgagatcc acgagccgtc cctgcaacac atgttcgatg gtcatgacgc
     4741 taagcacctg ccgcgggtcg tcgcgggcaa cgatggggag attcgcaatc tcgcgctcgg
     4801 ccatgagatt tgcaagctcg cgcagcgtca tgtcggggta gccgagcacc atctcgcgaa
     4861 tcatcacatc gtcgaggcgg gtgtcgggtg cgccctcgag ttcgaaggcg gcatcgagca
     4921 gttggtggcg ggtgatgagc ccgatcatgc gatcgtcgcc gtcgagcacc ggatagaggc
     4981 gctggccgtg ttcgagcttg cgcttgccgc cgcccgacca ggcgcccgcc gcctcggtcg
     5041 ccgcttcgcg cagactcgtg cccgagcgga agctcacgaa gttgcgcggc atgatctcgc
     5101 cgacgagaaa tacatcgagc ggatccaccg agtactcgcg ggtcacatgg gtgcccttgc
     5161 gcacgacttt ttccgtgagc accgaacgct tgagcaccag agtggaaagc aggaacgcgg
     5221 ccgtcgaggc gacgacgagc gggagaagcg catcccattg gtgcgtaagt tccatcgcga
     5281 agatgacgcc ggtcaaggga ctgcgcatca cgccgccgac gaccgccgca aggcacatga
     5341 gcgaccagaa gccgggcgcg acgtgcggaa agacgtagcc gagcacggcg ccgagcgcgg
     5401 cgccgatcat gaacaccggc gcgagcacgc cgcccgacgt gccggagccg agcgaaaggc
     5461 tccagatgag ggtcttcacg accagaatgc cgacgatcag cgcgagcgtt gcctggcctg
     5521 cgaggagttc ccggatcacg tcgtagccga cgccgagcgc ccgcggttcg aagagcccgc
     5581 cgacgccgat gatgagtccg ccgatcgccg gccaccacat ccagtggatc ggaagcttgc
     5641 ggaacgcgtc ttcggagaga tagacgagta gcgtcgcgag ccatgcgaca aagccgccga
     5701 cgagcccggc gaccacgcac agacacagca cgatcgggct caccgtcggc agggcgtgac
     5761 tcgtggcgaa gatcggcgcg aaaccgagaa gaggccagcg gagcgccgtc gcgacgacga
     5821 ccgcgatcgt caccggcacg aaggagcgcg ggcgcatctc gaagagcagc agttcgaccg
     5881 cgagcaggat cgaggcgagc ggcgcgttga aggtggccgc catgccggcc gcggcgccgg
     5941 cgacgaggag cgtcttgcgt tcgtcggccg tgagcgagag cgtctgcgcg agcagcgaac
     6001 cgaaggcgcc gccggtcatg atgatcgggc cctcggcgcc gaagggaccg ccggtgccga
     6061 tggtgatcgc agccgaaatc ggcttgagga cggcgatgcg cgggcggatc ttgctgccgc
     6121 cgcgcaaaat cgcctcgatc gcctcgggca tgccgtggcc gcggatcgcc tcggcgccgt
     6181 agcgcgccat gaatccgacg atcaggccgc cgacaacggg agccgcgatg atgagccacc
     6241 actgatcgtg ccctccgctc ggcgcgacca gcgacagggc gacgcgctgg tagaagacga
     6301 gattggtgat gagtccgatc agcttcagca gggcatcggc gacgagcgcg gccgcggcgc
     6361 cgacgacgag cgcgagaccg gagaggagaa gaatgcgcgg attgacgttg aagtcgccga
     6421 ggcggcgcgg tacccgcggg cgggcgacgg cgatcgccga gtccggattg ttcatcttcg
     6481 ttggaatcga gcttcccccc aagcctgccc gcgcggaaaa ggtttcggcg ctccctcagt
     6541 ggaaggagta gccgaaggcg gcctcgaagc gcgccgcgaa ttccgcgggc gtgaagcggt
     6601 gattctgggt gccctcgctc gcgatcttga tcgagcccgc gagcgaggcg atgcgccccg
     6661 tggtttccca ctccagatcg tgcagcaatc cgtagagcaa tccggcgcga taggcgtcgc
     6721 cgcagccggt gggatccacc accgcgttcg gttcgacggc cgggatatcg tactgattgc
     6781 cttcggcgtg aaccaccgag ccgtgcgcgc cgcgcgtcac gatcacggcc ttcaacttgt
     6841 ccgcgatctc gtcgaggccg aggcgagtct tttcgcaaaa gacgccggcc tcgtaatcgt
     6901 tcatggcgag ccagctggca cgttgcaaaa aatactcgag atcggagccg tcgaacatcg
     6961 gcagcccctg gcccggatcg aaaaggaagg gaatgcccgc ggcggcgaat tcttcggcgc
     7021 gccggatcat ggcgtcgcga ccgtcggggg cgacgatgcc gatgcgcgcg tcggctatcc
     7081 gcggtagcgt ctgttcgtgc gcgcgcgcca ttgccccggg ataaaaggcg gtgatctggt
     7141 tgccgtcgtt gtcggtattg atgaaagcct gtgcggtgta cacgtcgccg agttccagaa
     7201 ttgattcgcg cgcgatgccg ttctggtcca gccacttggc atagggcgcg aagtcgcggc
     7261 cggcggcgcc gagcggcagc gcgcgctcgc cgagcagcgt gagcgtgtag gcgatattgc
     7321 ccgcacagcc gccgaactcg cgccggagtt cactcaccat gaaggcgacg gacagcttgt
     7381 ggatgcgctc aggcaggatg tggtcgccga agcgcccggg aaaacgcatg atgacatcgt
     7441 aggcgaggga acccgaaatg actgcgctca tgagccgagg tctccctgcc aggcgaggtc
     7501 cagttcgcgc gcggccttga cgtcgtcgag gcggccgacc ggcgtcgtgt agggggcgcc
     7561 cttgagtttt tccggttcgt cttgcgcctc ggcgagaatc gccgccatcg cgtccacgaa
     7621 ggcgtcgagc gtttccttgg actcggtctc ggtgggctcg atcaggagac attcggggac
     7681 gagcagcgga aagtacgtgg tcggcgcgtg aacgccgtaa tccagaagac gcttggcgaa
     7741 atccatcgcc gtgatgccct gggtcttcgc gagtttcttc agcgtaacga tgaactcgtg
     7801 actcgcgcgc cggccgggat aggcgagttc gaagccggcg gcgacgagac gcgcggcgag
     7861 ataggcagca ttgagcgtcg cgtattcggc gacccgctgc atcccgtccg cgccgagaag
     7921 gcggatatag acgtaggcgc gcagcagcac cccgacgttg ccgttgaagg ccgaaagccg
     7981 cccgatggat tgcggccgat ctttctcgtc gagcaaacga tagcgctcgc ccgccttcgc
     8041 gaccagcggc agcggcagaa agggttcgag ccgcgggccg acggcgaccg agccggcgcc
     8101 gggaccgccg cccccgtggg gcgtcgaaaa ggtcttgtgc agattgaagt gcatgacgtc
     8161 gaagcccatg tcgccggggc gcaccttgcc gagaatcgca ttcatgttgg cgccgtcgta
     8221 atagaggagc ccgcccgcgg catgcacgat cttcgcgatc tcgccgatgc gccgctcgaa
     8281 gactccgagc gtcgagggat tggtgagcat gatgcccgcg gtcttctcgc cgacggcggc
     8341 cgcaagcgcc tcgaggttca cgtcgccgtc gcccgcggtc gggatttcgc gcacggtgta
//
LOCUS       DASUJG010000341         4732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_954230, whole genome shotgun sequence.
ACCESSION   DASUJG010000341 DASUJG010000000
VERSION     DASUJG010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4732
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954230"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1428
                     /locus_tag="VFX38_07405"
     CDS             <1..1428
                     /locus_tag="VFX38_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX5314710.1"
                     /translation="PWGDASTSRGGYHLVWSRDLVECAGALIALGAVEEARRVLTYLL
                     ATQQADGHWLQNQWLGGKPFWQGVQLDETAFPVLLAGLLRDCQALDGIPVAGMVRRAL
                     AYLIANGPASSQDRWEEDAGINAFTLAVMIAALVEGSQFLEEPDRGTALMIADTWNAR
                     IEDWTWAEGTELAQKLGVPGYYLRIAPADVLTREDAKSEDMLIKNRSYDNHLDANDQV
                     ATDFLQLVRYGLRRADDPKIVASVRVMDQLLRSDTPNGPVWHRYNNDGYGEHRDGRPF
                     DGTGHGRGWPLLTGERGHYALAAGEDPLPYLEAMAAMTGPGGLIPEQVWDSDPISERG
                     LYPGKPSGSAMPLVWAHGEFIKLCVSREMHRPCDRPPLTWARYQGKRPTIDWRDWRLH
                     LAPRRLPAAHTLRLLLPEAAQVHWGVNGWQRIADVETHPVGLGLHQADLPTKKLPVGT
                     TVDLTFRWLDRDTWQGEDFAVEIIA"
     gene            1520..2095
                     /locus_tag="VFX38_07410"
     CDS             1520..2095
                     /locus_tag="VFX38_07410"
                     /inference="COORDINATES: protein motif:HMM:NF017957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF996 domain-containing protein"
                     /protein_id="HEX5314711.1"
                     /translation="MDKTTKLLGGWGYIGVIVFGLFGGFLGPVAILSLVGAIIVVISY
                     VRAAGEYNRPEIRTNAIVALIIAIVSVIVFIFMVGASIFSLLFHAGEASIAAFGGSLI
                     AGVLITWLLAIVASWFWFRASASLGAASGQNLFKVGGLLIFIGAITIVVFGIGGIVSL
                     IGEILITVAFFATPEKSGAQVVVASDSAPSV"
     gene            complement(2139..2807)
                     /locus_tag="VFX38_07415"
     CDS             complement(2139..2807)
                     /locus_tag="VFX38_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX5314712.1"
                     /translation="MAKELRQHYLFRNLNEAQLARILARSRERAFERGERLFSRGEPA
                     ATFFLLRRGSAKLYRVSREGQEKVMRFIRPPQSFAETVMFMDVPRYPVHGDGVQPGVL
                     LAIDCAAFLDILRESFATCRAVMAQMTVRIQAHWDEIEELTLENSRFRVVHYLLGLAP
                     DGGSGPVTVTLPARKLLIASQLAITPETLSRILRSLSEEGLIEVQAAQVTLRDVAALR
                     RHPR"
     gene            complement(3261..>4732)
                     /locus_tag="VFX38_07420"
     CDS             complement(3261..>4732)
                     /locus_tag="VFX38_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEX5314713.1"
                     /translation="WTVASPDLSGADANAKDCADAQNSLAAAKACGFGVIFTIAPSPK
                     DSGTIWVGTDDGQVQLTTDGGKHWRNVTPPAMPLWGKVNDIAPSPFDADTAYVAVDTH
                     RLGEHRPVIFRTTDAGAHWQTITAGLPADEFVQTVVADSGRRGLLFAGSDRSVYVSFS
                     DGDGWQPLSLNLPTTSIRSLTIHGDDLVAATMGRGFWSLDDIEPLREGTANIAASPAH
                     LFAPAVAIRLRASENRDTPPPPSEPRAKNPPTGAIIDYWLKDATSGPVTLTISDSSGH
                     VLRQFSSAAKPERLDADRYFEKSWVGAPETLSAAAGAHRFVWNLRWPRPPAISYQYSI
                     SAVWPGDTPLVPQGALALPGKYTMTLTVDGKRYTQPLTIKMDPRVDVGEQALAANLAL
                     ENKISADLARAIAAYDASGATLSELKQHGGNAAQVKALEAWRDTDENSLANVAGVLDT
                     LATNLEMADAAPTAGQRAVYAAYSQKLDALLTHRPKPGS"
BASE COUNT          772 a         1579 c         1550 g          831 t
ORIGIN      
        1 ccgtggggcg atgccagcac cagccgcggc ggctatcacc tggtgtggtc gcgcgacctc
       61 gtggagtgcg ccggcgcgct tatcgccctc ggcgccgtcg aggaggcccg ccgggtgctg
      121 acctatcttc ttgccaccca gcaggcggat ggccattggc tgcagaacca atggctcggt
      181 ggcaagccgt tctggcaggg cgtccagctc gacgagaccg ccttcccggt gctcctcgcc
      241 ggactccttc gcgactgtca ggcgctcgac ggcatcccgg tcgcgggaat ggtgcggcgc
      301 gcgcttgcgt atctcatcgc caacgggccg gcgagttcgc aggatcgctg ggaagaagac
      361 gccggcatca atgccttcac cctcgcggtg atgattgccg ccctcgtcga aggctcgcag
      421 tttctggaag aacccgatcg cggcacggcg ctcatgatcg ccgacacctg gaatgcccgc
      481 atcgaggact ggacctgggc cgaaggcacg gaactcgccc aaaagctggg agtgccgggc
      541 tactacctgc ggatcgcgcc cgccgacgtc ctcacccgcg aggacgccaa gtccgaggac
      601 atgctgatca agaaccgctc ctacgacaat cacctcgatg ccaacgatca ggtcgcgacc
      661 gatttcctgc agctcgtgcg ctacgggctg cgccgcgcgg acgatccgaa gatcgtcgcg
      721 agcgtgcggg tgatggacca acttctgcgc agcgacacgc cgaacggccc ggtctggcat
      781 cgctacaaca acgacggcta cggggagcac cgcgacgggc ggcccttcga cggtaccggt
      841 cacgggcgcg gctggccgct gctcacgggc gaacgcgggc actacgcgct cgcggcgggc
      901 gaagatccgc tcccatatct cgaggcgatg gcggcgatga ccggccccgg agggctcatc
      961 cccgaacagg tctgggacag cgatccgatt tccgaacgcg ggctctatcc cggcaaaccg
     1021 agcggctcgg cgatgccgct cgtatgggcg cacggggagt tcatcaagct ctgcgtcagc
     1081 cgcgagatgc atcggccctg cgaccgcccg ccactcacct gggcccgcta tcagggcaag
     1141 cgcccgacga tcgactggcg cgactggcgc ctgcatctcg caccgcggcg cctgcccgcc
     1201 gcgcatacgc tgcgcctgct cctccccgaa gcggcgcagg tgcactgggg cgtgaacggc
     1261 tggcagcgca tcgccgacgt cgaaacccac cccgtcggtc tcggcctgca tcaggccgat
     1321 ctgccgacga agaagcttcc ggtcggcacc accgtcgact tgaccttccg ctggcttgat
     1381 cgcgacacct ggcagggcga ggacttcgca gtcgaaatca tcgcctgagc gcggcgcccg
     1441 gaggaaggct tgccttgcgc cgcgagcgcg ggtatagtca gccgcaccac ttctggcctg
     1501 tccccggagg gatgctcgca tggacaagac caccaagctg ctcggcggct ggggttatat
     1561 cggcgtaatc gtgttcggcc tgttcggcgg atttctcggc cccgtggcca tcctgtcgct
     1621 ggtcggagcc atcatcgtag tcatctccta cgttcgcgcg gccggcgaat acaaccgccc
     1681 cgagatccgc accaacgcga tcgtcgccct gatcatcgcg atcgtctcgg tcatcgtatt
     1741 catcttcatg gtcggcgcct cgattttctc gcttctgttt cacgccggcg aggccagcat
     1801 cgcagccttc ggcggaagcc tgatcgccgg tgtgctcatc acctggctgc tcgccatcgt
     1861 cgcgagctgg ttctggttcc gggcctccgc cagtctgggc gcggcgagcg gtcagaatct
     1921 gttcaaggtg ggcggcctgc tgatcttcat cggcgcgatc acgatcgtcg tcttcggaat
     1981 cggcgggatc gtttcgctga tcggcgaaat cctgatcacg gtcgccttct tcgccacacc
     2041 ggagaaaagc ggcgcccagg tcgtcgtcgc atcggactcc gcaccctcgg tctgaacctc
     2101 gcgccccgga gccaagccgg ggcgttgagc accgtctctc agcgcggatg ccggcgcaga
     2161 gccgccacgt cgcgcagggt aacctgcgcg gcctgtactt cgatgaggcc ttcctcgctc
     2221 aggctgcgca gaatgcgcga gagcgtttcc ggcgtaatcg cgagttgcga ggcgatcagg
     2281 agcttgcgcg ccggaagcgt cacggtgacc gggccgctcc cgccgtccgg cgcgaggccg
     2341 agaagataat ggacgacgcg aaagcggctg ttctcgagcg tcagctcctc gatctcgtcc
     2401 caatgcgcct ggatgcgcac ggtcatctgc gccatcaccg cgcggcaggt ggcgaaggat
     2461 tcgcgcagaa tgtcgagaaa ggccgcgcag tcaatcgcga gtaacactcc cggctgcacg
     2521 ccgtcgccgt gcaccgggta acgcggaacg tccatgaaca tcacggtttc ggcaaagctc
     2581 tgcggcgggc ggatgaagcg catgaccttc tcctgaccct cgcgcgagac ccgatagagt
     2641 ttcgcgctgc cccgccgcag caggaaaaag gtcgccgccg gctcgccccg gctgaacagc
     2701 cgctcgccgc gctcgaaggc ccgctcgcgg ctgcgcgcga gaatccgcgc cagttgcgct
     2761 tcgttcaggt tgcggaaaag ataatgctga cgcagctcct tcgccattgt ctccacgccc
     2821 gccaccatgc atttctctcc tgctcgtggc cgccagctta atagcggccg ggcgccgcgg
     2881 caaggagggc gcgcggcgta gcctgctcca tactcccggg gtcgcccgcc cgcgagcctc
     2941 ctcatgggca ggttcgttgg ctgaattttg ctttcgtttt tcgcgccggg tcttcacacc
     3001 ggctccggcg tgaagacgat tcccgccgtg cgtgaggcga ttgagcagaa gcagtagaac
     3061 gtcgcgcggc aatacgttcc cgtcgccacg acgctggacg gctacagcgc ccgactcgac
     3121 accgccacca aactgctcgg gtcaaagatc tgattgtcac gcacgattct ccgctcatcg
     3181 gatgatgcaa tggtaggagc gagcgataat ttgcactcaa gatcgcgagc aagctcgctc
     3241 ctgcaacagc gtttccatgc tcaggagccc ggtttcggcc gatgcgtgag aagcgcgtcg
     3301 agtttctggc tgtaggcggc gtaaacggca cgttgtccgg ccgtcggtgc ggcgtccgcc
     3361 atttcgaggt tcgtcgcgag cgtgtcaagc actccggcga cgttggccaa cgaattttca
     3421 tcggtgtcgc gccaagcttc gagcgccttg acttgcgcgg cattgccacc gtgctgtttc
     3481 aattcgctga gggtcgcacc gcttgcgtcg taggcggcga tcgcgcgggc gaggtcggcg
     3541 gagattttgt tttcgagcgc gagattggcg gcaagcgcct gctcgcccac gtccacgcgc
     3601 gggtccatct tgatcgtaag cggctgcgtg taacgcttgc cgtcgacggt caacgtcatg
     3661 gtgtattttc cgggcagcgc cagcgcaccc tgcgggacga gcggcgtgtc gcccggccac
     3721 acggccgaga tcgaatactg atagctgatc gcgggcggcc gcggccagcg cagattccac
     3781 acgaagcggt gcgcacccgc cgcggccgaa agcgtctcgg gcgcgccgac ccagctcttt
     3841 tcaaaatagc gatccgcatc gagccgttcg ggttttgccg cgctcgaaaa ctgtcgcagc
     3901 acatgaccgg aggaatcact gatcgtgagt gtgaccggac cgctggtcgc atccttcagc
     3961 caataatcga tgatcgcgcc ggtcggcgga ttcttcgctc tcggttcgga gggcggcggc
     4021 ggggtgtcac ggttttcgct cgcccgcagg cggatcgcga cggcgggcgc gaagagatgc
     4081 gcgggcgatg ccgcaatgtt cgcggtgcct tcgcgcaagg gttcgatatc gtccagcgac
     4141 cagaagccgc ggcccatggt cgcggcgacg agatcgtcac cgtggatcgt gagcgagcgg
     4201 atcgaggtgg tcggcagatt caagctcagt ggctgccagc cgtcgccgtc ggaaaacgac
     4261 acatagacgc tgcggtcgct gcccgcgaag agcagcccgc gacgtccgga atcggcgacg
     4321 accgtttgca cgaactcgtc ggcaggcagg ccggccgtga tcgtctgcca atgcgcgccc
     4381 gcgtcggtgg tgcgaaagat caccgggcga tgttcgccga ggcgatgcgt atccaccgcg
     4441 acataggcgg tgtcggcatc gaagggagac ggcgcgatat cgttcacctt gccccacagc
     4501 ggcatcgcgg gcggcgtgac gttcctccag tgcttgccgc cgtcggtcgt gagttgcacc
     4561 tggccgtcgt cggtgccgac ccagatcgtg ccggaatctt tgggcgacgg cgcgatggtg
     4621 aagatcacgc cgaagccgca ggccttggcc gccgcgagcg aattctgggc gtcggcacaa
     4681 tccttggcgt tggcatccgc gccgctcaga tccgggctcg cgacggtcca gt
//
LOCUS       DASUJG010000342         2179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_220815, whole genome shotgun sequence.
ACCESSION   DASUJG010000342 DASUJG010000000
VERSION     DASUJG010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220815"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VFX38_07425"
     CDS             <1..416
                     /locus_tag="VFX38_07425"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX5314714.1"
                     /translation="ERSQLEGPVVDIAPNQAIALAMAFHELATNAAKYGALSAAGGCI
                     EVHWRFDARGDARILHIDWTEQGGPPVGEPKRYGFGMRLVREGLSYQLEATVNLDFAP
                     DGLHATIAFPLKPIDLGAKIPRSGNGAAMRNGDGE"
     gene            406..786
                     /locus_tag="VFX38_07430"
     CDS             406..786
                     /locus_tag="VFX38_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010217215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5314715.1"
                     /translation="MANEAARPIVLAGMRILVVEDEMLLALALEDLLERKKCSVHKAP
                     SVAGALAQIADNPLDFAFLDINLGGEQVYPVADELQRRGVPFAFMTGYSSTAIEEAWR
                     GRPLLSKPFDEKALEAVVAQASAA"
     gene            910..1053
                     /locus_tag="VFX38_07435"
     CDS             910..1053
                     /locus_tag="VFX38_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314716.1"
                     /translation="MIAAKIVAATDAIMARAQALAFRILAERIEHREAAPLPVTFRLP
                     AAA"
     gene            1050..1263
                     /locus_tag="VFX38_07440"
                     /pseudo
     CDS             1050..1263
                     /locus_tag="VFX38_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005373476.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            complement(1310..>2179)
                     /locus_tag="VFX38_07445"
     CDS             complement(1310..>2179)
                     /locus_tag="VFX38_07445"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX5314717.1"
                     /translation="ALRLPVPCMNILNGGVHANWEGPDFQEYMIAPVGAESFGEALRR
                     GAEVYHALKSELKEMGLGTTVGDEGGFAPKVASNEQPLELIVKAIEQAGCKPGTEIAI
                     ACDAAASEFFSGGRYRLRSEKRELDAGEMSEYLGKLASTYPIVSVEDGLAESDWEGWK
                     NLTQALGGKLQLVGDDIFCTNPEIIRRGIAEGVSNAVLIKLNQIGTLTETIEATRLTL
                     DHGWGAMISHRSGETVDSFIADLAVALGSGQIKTGAPARGERVEKYNQLLRIESALGG
                     AAVYAGASAFAKH"
BASE COUNT          358 a          702 c          720 g          399 t
ORIGIN      
        1 aggaacgctc gcagctcgag ggacccgtcg tcgacatcgc gcccaatcag gcgatcgcgc
       61 tcgcgatggc cttccatgaa ctcgcgacca atgcggcgaa gtatggcgcg ctttctgcgg
      121 cgggcggatg catcgaggtg cattggcgtt tcgacgcgcg cggagacgct cgcatccttc
      181 acatcgactg gacggagcag ggcggcccgc cggtcggaga accgaagcgc tacggcttcg
      241 gaatgcgcct cgtgcgcgaa ggcttgagct atcaactcga ggcgacggtg aatctcgact
      301 tcgcccccga cggactgcat gccacgatcg cctttccgct caagcccatt gatttggggg
      361 caaagattcc gcgcagcggc aacggagccg cgatgaggaa cggtgatggc gaatgaggcg
      421 gcgcggccga tcgtgctggc gggcatgcgc atcctcgtcg tcgaagacga aatgttgctc
      481 gccctcgcgc tcgaggatct gctggaacgc aagaagtgca gcgtgcacaa ggcaccgagc
      541 gtggccggcg cccttgcgca gatcgcggac aatccgctcg acttcgcctt cctcgacatc
      601 aatctcggcg gggagcaggt ctaccccgtc gccgacgaac tgcagcggcg cggcgttccc
      661 tttgcgttca tgaccggcta cagttcgacc gcgatcgagg aggcctggcg cgggcgacct
      721 cttctttcaa agcccttcga cgaaaaggca ctcgaagcgg tcgtcgcgca ggcctccgcc
      781 gcctgaccga tccgctccgg gatcgcagca ccatctgcgc tcgccccggg ctccgccggg
      841 acgagtaatg cgaaatgggc gccgggtagt cggaatcagt tgtcagtacc ggggcgtgca
      901 tgcaggctcg tgatcgcggc gaagattgtc gccgcgaccg atgcaatcat ggcccgggcg
      961 caagcgctcg cgtttcgcat actcgccgag cgcatcgaac atcgggaagc ggcgccgctt
     1021 cccgtgacct tccggcttcc cgccgcggca tgagcgagtg gccttcggtt cgcgggcgcc
     1081 gtttgctcaa ggctgctcct gccgatcgga tggcagggca aacgacagtc tggttcgcac
     1141 cgcacactgt gccgcgccgg ccggccggac tacacgttcg cattccatga cagcgaagaa
     1201 atcggcccgc acatgctcgc gcgcgtcgcg aagcataccg gcctcgatcc cggtgactcg
     1261 tagaggcggt tacaggcgca caggcgatga cggcgggccg gcgcccgtct cagtgttttg
     1321 cgaatgcgct cgctcccgca tagacggcgg caccgccgag cgcggattcg atgcgcagca
     1381 gttgattgta tttttccacg cgctcgccgc gcgccggcgc gccggtcttg atctggccgc
     1441 tgccgagcgc gacggcgaga tcggcgatga agctgtccac ggtttccccc gaacgatgtg
     1501 aaatcatcgc tccccagccg tgatcgagcg tgaggcgcgt ggcttcgatc gtttcggtca
     1561 gcgtaccgat ctggttgagc ttgatgagca cggcgttcga gacgccttcg gcgatgccgc
     1621 ggcggatgat ctcgggattg gtgcagaaga tgtcgtcgcc gacgagctgg agttttccgc
     1681 cgagcgcctg tgtcaagttc ttccagcctt cccagtcgga ttcggcgaga ccgtcctcga
     1741 cgctgacgat ggggtaggtg ctcgcgagtt tgccgaggta ttcgctcatt tcgccggcgt
     1801 cgagttcgcg tttttcgctg cgcagacgat agcgtccgcc ggaaaagaat tcgctcgccg
     1861 ctgcatcgca ggcgatcgca atctccgtgc cgggcttgca gcccgcctgc tcgatggcct
     1921 tgacgatgag ttcgagcggc tgttcgttcg aagccacctt cggcgcgaag ccgccctcgt
     1981 cgccgacggt ggtgccgagc cccatctctt tcagttcgga cttgagcgcg tgatacacct
     2041 cggcgccgcg gcgaagcgcc tcgccgaagg attccgcgcc gacgggcgcg atcatgtatt
     2101 cctgaaagtc ggggccttcc caattcgcgt ggacgccgcc gttcaggatg ttcatgcagg
     2161 ggacgggcag acgcagggc
//
LOCUS       DASUJG010000343         2276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_708125, whole genome shotgun sequence.
ACCESSION   DASUJG010000343 DASUJG010000000
VERSION     DASUJG010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708125"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..1339
                     /locus_tag="VFX38_07450"
     CDS             146..1339
                     /locus_tag="VFX38_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314718.1"
                     /translation="MDLTPGVTVNALVELPPGFTPIAGEPPLWLQGAKEIALAGKVRG
                     RTEVLGFSGGNYKKMRLVAADGGPGAPHGKIVGLAASPDGQTLAVAEAEPGRIEIVLR
                     YVISNGGQNSVASFDGNFHAVSLNWLAPNTLAVGLAGDPAASPTEAGGLYLIHVLGAV
                     TVEQVKLTCPASMLVFSPDARFAVGEGDEHTPPALFDSRNGKCRHIGLDGPIRVLGWA
                     PGSTALLYSAPAGRDHGAGVFRYTIANGENELIAVSSSAAAYTGAGVVMALGNRDLNS
                     QSAASSPDKRVTAQIASFIPKQNQTLVDTLGMPTTPAMMMASTMTYSHGFAELALQLY
                     VAGPAGPTRKIVTFSIIDRKAFVLASGPFRGVAVIGWSPGANHLVIFDGDGTSGALAV
                     ITPQR"
     gene            complement(1386..>2276)
                     /locus_tag="VFX38_07455"
     CDS             complement(1386..>2276)
                     /locus_tag="VFX38_07455"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314719.1"
                     /translation="RGIRINKAASIGNVLMLEAADFLDLMTDDPATRAIGMYLEGVRD
                     GRRFFTSLRRAAARHPVVVWKGGMTEAGARATFSHTGSLATAAAVWRALMVQSGAVAV
                     ASLDAMLDAMELLARGRRVGGRGMGLVAMTGGQSVVITDTFASAGLEVPALSDASYAE
                     LKSFFNIIGGSYRNPLDAGGTIGMGHDQGNLDRILDILERDSAIDAIVLEIGTGLRAA
                     RWPVHEEELASLLDGLAEFNLRSHKPFAVILHPAHVAVIVARARELARGRGLVVFDSF
                     ERAAAAFGVAAEYWQGRRDS"
BASE COUNT          402 a          778 c          724 g          372 t
ORIGIN      
        1 aaactagcct ttttcaatgc accgatctct tccacccatc gttccacttc tcatgctgct
       61 aggtctcggc gccgcttcgt gctcgatatt cggccacaaa gaaaagcctc caggcccgcc
      121 gccagtcacg ttacacacgg cgacgatgga ccttacgccc ggcgtcaccg tcaacgccct
      181 ggtcgagctg ccgcccggct tcacgccgat tgcgggtgaa ccgccgctgt ggctgcaggg
      241 cgcaaaagag atcgcgctgg cgggtaaggt gcggggccgc acggaggtgc tcggcttcag
      301 cgggggcaat tacaagaaga tgcgcctggt cgccgccgac ggcggacccg gagcgccgca
      361 tggaaaaatc gtcggcctcg ccgcgagccc ggatgggcag acgctggcgg tggccgaggc
      421 cgagcctgga cggatcgaga tcgtactgcg ctacgtcatc agcaacggcg gacaaaactc
      481 cgtcgcaagc ttcgacggta atttccacgc ggtgagcctg aattggctcg cgcccaatac
      541 gctcgccgtg ggcctggcgg gcgatcccgc tgcttcgccg actgaagccg gcggcctgta
      601 tctgattcac gttctcggcg cagtcacggt cgagcaggtg aagctaacgt gcccggcctc
      661 gatgctggtc ttcagccccg atgcgcggtt tgcagtcggc gaaggcgacg agcatacgcc
      721 gccggcgctc ttcgacagcc gcaacgggaa gtgccgccat atcggcctcg atgggccgat
      781 cagggtgctc ggctgggcgc cgggaagtac cgcccttctc tactcggcac ccgctggacg
      841 cgatcacggc gcgggcgtct tccgctacac catcgctaac ggagagaacg agcttatcgc
      901 cgtttcctcg agcgccgcgg catacaccgg cgcgggcgtc gtcatggcgc tgggaaatcg
      961 tgacctgaat tcccaaagcg cggcgtcgtc gcccgacaag cgggtaaccg ctcagatcgc
     1021 gagcttcatt cccaagcaga atcagacgct ggtcgatacc ctcggaatgc caaccacgcc
     1081 ggcgatgatg atggcgagca cgatgaccta ctcgcacggc tttgccgagt tggcgctgca
     1141 attgtacgtg gccgggccgg caggcccgac gcgcaaaatc gtcaccttct cgatcatcga
     1201 ccgtaaggcg ttcgtgctgg caagcggacc gtttcgcggc gtcgcggtga tcggatggtc
     1261 acccggcgcc aatcatctgg tgatcttcga cggcgacggc acgagcggcg cgctcgcggt
     1321 gatcactccg caacgctaag gcggttctgc gcggcatcgg ttcaggcgcc ggggaaattc
     1381 gtacatcacg aatcgcgccg gccctgccaa tactcggcgg caacgccgaa cgcggccgcc
     1441 gcgcgctcga agctgtcgaa taccaccagc ccgcggccgc gcgccaattc cctggcgcgg
     1501 gcgacgatca ctgcgacgtg ggccggatgg aggatcacgg cgaacggctt gtgcgagcga
     1561 aggttgaact cggcgagccc gtcgagcaga ctggccagct cttcctcgtg aacgggccag
     1621 cgcgcggcgc gcagtcccgt tccgatctcc agcacgatcg catcgatggc cgaatcacgc
     1681 tcgaggatgt cgaggatgcg atcgaggttg ccctgatcgt ggcccatccc gatcgtcccg
     1741 ccggcgtcaa gcggattgcg atagctgccg ccgatgatgt tgaagaaact tttcaactcc
     1801 gcgtatgacg cgtcggaaag cgccggcact tcgagccccg ccgacgcaaa ggtatcggtt
     1861 atgaccaccg actgtccgcc cgtcatcgcg accagtccca tcccgcgacc gccaacgcgc
     1921 cgtccgcgcg ccagcagctc catcgcgtcg agcatcgcgt caaggctcgc gaccgcgacc
     1981 gccccgctct gcaccatcaa ggcgcgccat acggccgcgg cggtcgcgag cgagccggta
     2041 tgcgagaaag tcgcccgcgc cccggcctcg gtcatcccgc ctttccatac caccaccgga
     2101 tggcgcgcgg cggcgcggcg caggctcgtg aagaaacgcc ggccgtcgcg cacgccttcg
     2161 aggtacatcc cgatcgcccg ggtagccgga tcgtccgtca tcagatcgag gaagtcggcc
     2221 gcttccagca tcagcacgtt gccgatcgag gcggccttgt tgatacgaat gccgcg
//
LOCUS       DASUJG010000344         3893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_222054, whole genome shotgun sequence.
ACCESSION   DASUJG010000344 DASUJG010000000
VERSION     DASUJG010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222054"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..822
                     /locus_tag="VFX38_07460"
     CDS             55..822
                     /locus_tag="VFX38_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5314720.1"
                     /translation="MAEKAALVTGATAGFGEAIARAFLDAGWRVIGTGRRLQRLEKLR
                     AELGKDFLPLAFDVRDRSAVEKALATIPEYFLPIEALVNNAGLALGLERAHEADMADW
                     ETMVDTNVKGLLYMTHALAPGMVERGRGHVVNIGSIAADNPYPGGNVYGATKAFVWQF
                     SRNLRSDLLGTGVRVTDIAPGLAETEFSLVRFKGDETRARAPYRDLESLSGDDVARAV
                     LWVVTQPAHVNVNRIELAPTAQAWGAMATHRKKSKEP"
     gene            complement(837..1928)
                     /locus_tag="VFX38_07465"
     CDS             complement(837..1928)
                     /locus_tag="VFX38_07465"
                     /inference="COORDINATES: protein motif:HMM:NF025182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HEX5314721.1"
                     /translation="MSPRLAAAGANEAPPLPVVQSVTPLGTVQQNPVINMRDNAQSTL
                     FKGRSVWTFGDTDLSVPNAKGSYWDDNSLSWSDDLDASDGIRLEHDLADSSAVPREYL
                     PYTPAEARYNYNHDQKHCTQTPCGAEFALWSAQLVADPARDRLLLFYPEVWRVSGRPD
                     WRLVGSGIAVWTRAEGFTRPVENPGSPTPTLMWSDSEVSYIGGSLSVADTLYSYGCSP
                     DWIVMHCRLARVPLAGALDKSQWRYYAGGDNWSADENGAVTVFDGGAAANSVFYVPYL
                     GEYLAIYSQPFTDDVMYRVAEHPWGPWSEAGLLFTGLPGSQNNFDYSAVAHPEFSQGS
                     GRVQYVTYVRTTGFLQQEIRLVRVVFDDP"
     gene            complement(2047..>3893)
                     /locus_tag="VFX38_07470"
     CDS             complement(2047..>3893)
                     /locus_tag="VFX38_07470"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX5314722.1"
                     /translation="PGYKSADYPAAELAADALGSKRGPIQALQYQGKALFAGFQMQTL
                     PDVGIGYALAVYPPGGDANAVKQALIGAIGEVRKEGIASDLVSAAKRRAILAQALQKN
                     SIFGLAQTWTSALALAELDSPEEALARLREVTPEQVNAQVKDSLDLDHAITLVAEPTP
                     GTKPSSGSGFGGKESFASQPSGPVTLPDWAEQALATLPQPKPFLQPTDTRLANGMRLI
                     VQPLHISHSVSLYGIVNQNEDLQAPPGEEGVGDLLGSLFDYGPQGMSRLAFDAAQDKI
                     GANLTVGPSFSLEVLPQYFDQGVQLLASDLLQPALPEKAFQTQQFLQAQQAAGQVKSP
                     EFKFGRAVQEALLPKGDPALRLATAQTIGSLTLDKLQGYYDKAYRPDETTIVVIGDIA
                     PSAAKSAVEKYFGGWKATGSKPDLDYPPVPPSKATHTFVPDPMKKQDQVVLAETLDLD
                     YTDPDHFALDLANDYLSGGFYATPLYHVLREKLGLVYNVSASFYFARHRGNFTLNYGS
                     YPGKVTEARTAAIKVLQDASAKPFTADELHLAKSIGLRQIQLGQQSVDAIAQSWLTRS
                     TESLPLDWDYVMARHFEELTAPEIHRALQKYLDLSRLSTVVLGQPVKP"
BASE COUNT          725 a         1218 c         1276 g          674 t
ORIGIN      
        1 gccctgcggg cttacgccgg cctacaatgg agacccgagc acaggaagca gagtatggct
       61 gagaaggcgg cattggtgac gggcgcaacc gcgggattcg gcgaggcgat cgcgcgcgca
      121 tttctcgacg caggctggcg ggtaatcggt accggtcggc ggcttcaacg tctggaaaag
      181 ctccgtgcgg aactgggcaa ggactttctg cccctcgcct tcgacgtgcg cgatcgcagt
      241 gccgtcgaaa aggcattggc gacgattccg gaatatttcc tgccgatcga agcgctggtc
      301 aacaacgcgg ggcttgcgct cgggctcgag cgggcacacg aagccgacat ggcggactgg
      361 gagacgatgg tggacaccaa cgtcaagggg cttctctaca tgacccatgc gcttgcgccg
      421 ggcatggtcg agcgcggtcg cgggcatgtc gtgaacatcg gctccatcgc cgccgacaat
      481 ccctaccccg gcggcaacgt ctacggagca accaaggcgt tcgtgtggca gttctcgcgc
      541 aacctgcgtt cggatcttct cggaaccggc gtgcgcgtga ccgacatcgc accgggtctt
      601 gccgaaaccg agttttcgct ggtgcgtttc aagggcgacg aaacgcgggc gcgtgcccct
      661 taccgcgatc tcgaatcgct gagtggcgac gacgtcgcgc gcgcggtgct gtgggtagtc
      721 acgcaacccg cgcacgtcaa cgtgaaccgg atcgaactcg cgcccaccgc acaggcctgg
      781 ggcgcgatgg ccacgcaccg caaaaaaagc aaggaaccct gaaggcgaaa atccactcag
      841 ggatcgtcga agacgacccg cacgaggcgg atttcctgtt gcaaaaagcc ggtagtgcgc
      901 acgtaggtga cgtactgcac ccgcccgctt ccctgcgaaa actccggatg cgcaaccgcg
      961 gagtagtcga aattgttctg cgagcccggc aggccggtga agagcaaccc ggcctccgac
     1021 cacggccccc agggatgctc cgccacccga tacatcacgt cgtcggtgaa gggctgggag
     1081 taaatcgcca gatactcccc gagatacgga acgtagaaga ccgagttcgc ggcggccccg
     1141 ccgtcgaata ccgtcacggc gccattctcg tcggcgctcc agttgtcgcc gcccgcgtaa
     1201 tagcgccact gcgatttgtc cagcgccccg gcaagcggca ctcgcgccag ccggcaatgc
     1261 atgacaatcc aatcggggct gcaaccgtag gaatagagcg tgtccgcgac ggagagcgaa
     1321 ccgccgatat agctcacttc gctgtcgctc cacatcagcg tcggcgtcgg cgaacccggg
     1381 ttttccacgg ggcgcgtaaa gccctcggcg cgggtccaca ccgcaatgcc cgagccgacg
     1441 agccgccaat cgggccggcc gctgacgcgc cagacctcgg gataaaacag cagcaggcga
     1501 tcgcgtgcgg gatcggcgac aagctgcgcc gaccacaggg caaactcggc tccgcacggg
     1561 gtctgcgtgc agtgcttctg gtcgtggttg tagttgtagc gggcctcggc cggcgtgtag
     1621 ggcaggtatt cgcgcggcac agcgctggaa tcggcgagat cgtgctcgag gcggatgccg
     1681 tcggaggcat cgagatcgtc gctccaggaa agcgagttgt catcccagta gctgcctttc
     1741 gcgttcggta ccgacaggtc ggtatcgccg aaggtccaga ccgagcggcc cttgaaaagc
     1801 gtgctctggg cgttatcccg catgttgatc acagggttct gctggacggt gccgagcggc
     1861 gtgaccgatt gcacgacggg cagcggcggc gcctcgttcg cgccggcggc cgcaagacgc
     1921 ggtgacaacg caaacgcgca tgcagccgcg caaaccgcga acggcaatat gcgctgccgg
     1981 caactcacgg attctctccc ggaacgacga ggatcgaggc cgagggcgga aaacgcggcg
     2041 cgcccgttac ggcttcaccg gctggccgag cacgacggtc gaaagtcgcg acaggtcgag
     2101 atacttctgg agcgcccggt gaatctcggg tgcggtcagt tcctcgaaat gacgcgccat
     2161 gacgtagtcc cagtcgagcg gcaggctctc ggtgctgcgc gtgagccagc tctgcgcgat
     2221 cgcatcgacg ctttgttgac ccagttgaat ctggcgcagg ccgatggact tcgccagatg
     2281 caattcatcc gccgtgaacg gcttcgcgga cgcgtcttgc agcaccttga tcgccgccgt
     2341 gcgcgcctcc gtcaccttgc cgggataaga gccataattc aacgtaaaat tcccgcgatg
     2401 gcgcgcgaaa tagaaactcg cactgacatt gtaaacaaga cccagcttct cgcgcagaac
     2461 gtgataaagc ggagtcgcgt aaaagccgcc ggaaagatag tcgttcgcga gatcgagcgc
     2521 gaagtgatcc ggatcggtgt agtcgagatc gagcgtctcg gcgaggacca cctgatcctg
     2581 cttcttcatc ggatcgggta cgaaggtatg cgttgccttc gacggcggta ccggcggata
     2641 gtcgagatcg ggcttcgatc ccgtcgcctt ccagccgccg aagtatttct cgaccgccga
     2701 cttcgccgcc gacggagcaa tgtcgccgat cacgacgatc gtcgtctcgt cgggccggta
     2761 ggccttgtcg taatagcctt ggagtttgtc gagcgtgagg gaaccgatgg tctgggccgt
     2821 tgcgagacgg agcgccggat ctcctttggg aagcaatgcc tcctgaaccg cacgcccgaa
     2881 cttgaactcg gggctcttca cctggccggc ggcctgttgc gcctgcagga attgctgcgt
     2941 ttgaaacgcc ttctcgggaa gcgccggttg cagcagatcg ctcgcgagaa gttgcacacc
     3001 ttgatcgaag tattgcggca agacctcgag cgagaagctc ggcccgacgg tgagattcgc
     3061 accaatcttg tcctgggcgg catcgaacgc aagccggctc atgccctgcg ggccgtaatc
     3121 gaagagcgag ccgagcagat cgccgacgcc ttcttcgccc ggtggtgcct gcagatcctc
     3181 gttttggttc acgatcccgt agaggctcac cgaatggctg atgtggagcg gttgcacgat
     3241 gagacgcatc ccgttcgcaa gccgtgtgtc ggtcggttgc aggaagggct tcggttgcgg
     3301 aagcgtggca agcgcctgct cggcccaatc cggaagtgtc acggggccgc tcggttggct
     3361 cgcgaacgat tccttgccac cgaagccgct gccgctcgag ggcttggttc cgggcgtggg
     3421 ctccgcgacc agcgtgatcg catgatcgag atcgagcgaa tccttcacct gcgcgttaac
     3481 ttgttcgggc gtgacctcgc gcaggcgcgc gagcgcctcc tcgggcgaat cgagttcggc
     3541 gagggcgagc gcgctggtcc aggtctgggc gaggccgaag atcgagtttt tctgcagcgc
     3601 ctgggcaagg atcgcgcgcc gtttggcggc gctcaccaga tcggacgcaa taccttcctt
     3661 gcgcacctcc ccgatggcgc cgatcagcgc ctgcttcacc gcattcgcgt caccgccggg
     3721 cggatacacg gccagcgcat agccgatgcc gacatcggga agggtctgca tctggaagcc
     3781 ggcgaagagt gccttgcctt gatactgcag cgcctggatc ggcccgcgct tggagccgag
     3841 cgcatcggcg gccagctccg ccgccggata atcggcgctc ttgtagcccg gca
//
LOCUS       DASUJG010000345         4282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1077055, whole genome shotgun sequence.
ACCESSION   DASUJG010000345 DASUJG010000000
VERSION     DASUJG010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077055"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..171
                     /locus_tag="VFX38_07475"
     CDS             <1..171
                     /locus_tag="VFX38_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003817612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX5314723.1"
                     /translation="WIAGSRSIADPYLYVMLRWAKAQKIDLSGLDNLSGFVARIEADP
                     AVQKVLAAEKRA"
     gene            194..1993
                     /locus_tag="VFX38_07480"
     CDS             194..1993
                     /locus_tag="VFX38_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEX5314724.1"
                     /translation="MMVQHGRTLRSLAPFLWEFRGRAAVAIVFLIAAKLATVAIPMLL
                     KHIVDALDGTNVAIVLPLALLLAYGALRFAATLFGELRDLVFEFAALRAVRRVALRVF
                     RHLHDLDLAFHLERQTGGLSRDVERGTRGISTVLSFLLFNILPTLVEIGLVVGIFLWN
                     FPVWFALIILGAVILFIAYSLVITEWRMGHVRRMNELDSRANTRAIDSLLNYETVKYF
                     TNEEFEAGRYDAEMARWEREALKTQYSLSTLNIGQALIIALGVTAMMILAGYETVQRR
                     MTIGDFVMVNAYMLQLFTPLNFLGFVYRQMKHAFTDMERMFTLLDRRNAVADAPGAPA
                     LAVLRGELRFENVHFGYGPDREILHGLSFTVPAGAKVAVVGPSGAGKSTLARLLFRFY
                     DVGAGRILVDGVDIREVSRASLRENIGIVPQDAVLFNDTIGYNIAYGRTGASAEEIAG
                     AAQLAHLRDFVESLPRGYDTLVGERGLKLSGGEKQRVAIARAMLKNPPILVFDEATSS
                     LDSNAEQAILAAMRELAQDRSSLVIAHRLSTVVDADEIVVLDGGRIVERGSHRDLLRY
                     GGLYAELWTLQQSEARNGDREQTKRADAFAPAL"
     gene            complement(2017..2568)
                     /locus_tag="VFX38_07485"
     CDS             complement(2017..2568)
                     /locus_tag="VFX38_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314725.1"
                     /translation="MRKLILTAFLLTLGFAAAGVRAGAVSPADQKFFDDNIGKVVKPD
                     PTPLANPALAKMFTGRFYNVELNIAGAGEKIVVAREGNDLIAVTTPSTTADMPGFPKL
                     LKPDFRLKSDEDAHVLQDALDVLYPIDDSFGGEDAQAKTIRHAGNVWTFVRGKFFEHF
                     KGFVFTTDESGAVTKVQYSLDIK"
     gene            2719..3282
                     /locus_tag="VFX38_07490"
     CDS             2719..3282
                     /locus_tag="VFX38_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314726.1"
                     /translation="MTAGLGHYGWVWIVALAAILAFALYRRGRRLIGHQRVNDKRIFT
                     RIMLLAVLIALALSSYTRGANAVEGYEAAAAGFVLGVVVALAALRFTQMGRDKEGVWY
                     VPNLYLGIGLIGLLVARFAYEYFVVFPQIRQAAAEPGAVAAITVGPLIHGLLFVVLGY
                     YLIYYIGILWLARRLPPEPPHSMRERP"
     gene            3279..3971
                     /locus_tag="VFX38_07495"
     CDS             3279..3971
                     /locus_tag="VFX38_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017909988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX5314727.1"
                     /translation="MRTLGLIGGMSWESTAHYYARLNELVAERLGGLHSARLLLWNAD
                     FDEIAALQQADDWDGAGAILAAVAQRLESAGADALLICANTMHRVAPAIEAAVNIPLL
                     HLADITADALRRDGFRRAALLGTRFTMAQPFYRERLESHGLAIDVPNADEQEDIHRII
                     YEELVKGRFEPRSRERFLAIMKRMHATGSQAVILGCTEFGLLIRPEDAPEIPRFDTTE
                     LHCRAAANWMLA"
BASE COUNT          701 a         1372 c         1358 g          851 t
ORIGIN      
        1 tggatcgccg gcagccgctc catcgccgat ccgtatctgt acgtgatgct gcgctgggcg
       61 aaggcgcaga agatcgacct ttccggcctc gacaatcttt ccggtttcgt cgctcgcatc
      121 gaggccgacc ccgcggtgca gaaggttctg gcggcggaaa aacgggcctg agcgtttccg
      181 cgaggccgcg ccgatgatgg tccaacacgg ccgcacgctc cgcagcctgg ccccgtttct
      241 ctgggagttt cggggccggg cggccgttgc gatcgtgttc ctgattgccg ccaagctcgc
      301 gaccgtcgcg atcccgatgc tgctcaagca catcgtggat gcgctcgacg gcaccaacgt
      361 cgcgatcgtg ctgccgctcg cgcttcttct cgcctacggc gccctgcgat tcgccgcgac
      421 gcttttcggc gagcttcgcg atctggtgtt cgagttcgcg gcgctgcgcg ccgttcggcg
      481 ggtggcgctg cgcgtcttcc ggcatctgca cgacctcgat ctcgcctttc atctggagcg
      541 ccagaccggc ggcctttcgc gcgacgtaga acgcggcacc cgcggcatca gcaccgtgct
      601 gagtttcctg ctgttcaaca ttctgccgac cctggtcgaa atcgggctcg tcgtcggcat
      661 cttcctgtgg aacttcccgg tctggttcgc gctgatcatt ctcggggcgg tgattctctt
      721 catcgcctat tcactcgtca taaccgaatg gcgcatgggt cacgtccgcc gcatgaacga
      781 actcgattcg cgggcgaata cccgcgccat cgacagcctc ctcaactacg aaacggtcaa
      841 gtacttcacc aacgaagagt tcgaggcggg ccgctacgac gccgagatgg cgcgctggga
      901 gcgtgaggca ctgaagaccc agtattcgct ctcgacgctc aacatcggac aggccctgat
      961 catcgcgctc ggcgtcacgg cgatgatgat tctcgccggt tacgagacgg tccagcggcg
     1021 catgaccatc ggcgactttg tcatggtcaa tgcctacatg ctccagctct tcacgccgct
     1081 caattttctc ggcttcgtct atcgccagat gaagcatgcc ttcaccgaca tggagcgcat
     1141 gttcacgctg ctcgatcggc gcaacgcggt ggcggatgcg ccgggagcgc cggcgctcgc
     1201 cgttcttcgc ggcgagttgc gcttcgaaaa tgtacatttc ggctacgggc ccgatcggga
     1261 aattctccac ggcctgagtt tcaccgtgcc cgccggggca aaagtcgcgg tggtcgggcc
     1321 gagcggtgcg gggaagtcca cgcttgcgcg cctgcttttc cgtttctatg atgtcggtgc
     1381 gggacgcatt ctcgtggacg gcgtggacat tcgcgaggtc tcccgcgcaa gtctgcggga
     1441 gaacatcggc atcgtgccac aggatgccgt tctcttcaat gacacgatcg gctacaacat
     1501 cgcctatggc cgcaccggcg cgagtgcgga ggaaatcgcg ggcgcggcgc aactcgcaca
     1561 tctgcgggac ttcgtcgaat cgcttccgcg cggctacgac accctggtcg gcgagcgcgg
     1621 gctgaagctc tccggcggcg agaagcagcg ggtcgcgatc gcgcgggcaa tgctgaaaaa
     1681 tcctccgatt ctcgtcttcg acgaggccac ttcgagcctc gattccaatg ccgagcaggc
     1741 cattctcgcg gccatgcgcg aactcgcgca agaccgcagc agtctcgtca tcgcgcatcg
     1801 cctttccacc gtcgtggatg cggatgaaat cgtcgtgctc gatggcgggc gcatcgtcga
     1861 gcgcggcagt catcgcgacc tcctgcgata cggcggcctc tacgccgaac tctggacgct
     1921 ccagcaaagc gaggcgagaa acggagacag ggaacaaacg aagcgagccg acgctttcgc
     1981 gccggctctc tgacgacctc cgggcggcgt gcgcggctat ttgatgtcga gcgagtactg
     2041 gaccttggtg acggcgccgc tctcgtcggt cgtgaacacg aagcccttga agtgttcgaa
     2101 aaatttgccg cgtacgaagg tccagacgtt gccggcatgt cggatcgtct tggcctgcgc
     2161 atcctcgccg ccgaagctgt cgtcgatcgg gtagaggacg tcgagcgcat cctgcaggac
     2221 gtgcgcgtcc tcgtccgact tcaggcggaa atccggcttg agcagcttcg ggaagccggg
     2281 catgtccgcc gtcgtggacg gggtcgtcac cgcgatcaga tcgtttccct cacgggcgac
     2341 gacgattttc tctccggcgc cggcgatgtt cagttccacg ttgtagaaac ggccggtgaa
     2401 cattttggcg agcgccggat tggcgagcgg cgtcgggtcc ggtttgacca ctttgccgat
     2461 gttgtcgtcg aagaacttct gatcggccgg actcacggcc ccggcgcgga ctcccgcggc
     2521 ggcgaatccc agggtcaaca aaaaagcggt cagaatcaac ttgcgcatga ctgtccctcc
     2581 gattgtgatg gcgggcaaag cgcccgggcg aagtatagac tccgccgtgc ctttccgcaa
     2641 tgcgatcttc gcgtccgccc ggattcgagc cttcccggat atcatgggcg ccggtcggct
     2701 ttcgggacgg agatcggaat gacggcgggg ctcggacatt acggctgggt ctggatcgtt
     2761 gcgctggcgg cgatcctggc cttcgccctg taccgtcgcg ggcggcgact catcggtcac
     2821 cagcgtgtca acgacaagcg cattttcacg cgcatcatgc tgctcgccgt gctgatcgcg
     2881 ctggcgctca gctcctatac gcgcggtgcg aatgccgtcg agggatacga ggcggccgcc
     2941 gcgggtttcg tgctcggcgt cgtcgtcgcg ctcgccgcct tgcgcttcac gcagatgggt
     3001 cgggacaagg agggcgtgtg gtacgtgccg aatctttatc tcggcatcgg cctgatcggg
     3061 ctcctggtcg cgcgtttcgc ttacgaatat ttcgtcgtct tcccgcaaat ccggcaggcc
     3121 gcggccgaac ccggcgccgt ggccgcgatc acggtcgggc cgctgattca cggcctgctc
     3181 tttgtggtcc tcggctatta cctgatctat tacatcggca ttctgtggct cgcgcggcgg
     3241 ttgccgccgg agccgccgca ttcgatgcgg gagcgcccgt gaggacgctc ggcctgatcg
     3301 gcggaatgag ctgggagtcc acggcgcatt actacgcgcg cctgaacgaa ctcgttgccg
     3361 aacggctcgg cggtttgcat tcggcgcgtc ttctcctctg gaacgcggac ttcgatgaaa
     3421 tcgccgcctt gcagcaggcc gacgattggg acggagcggg agcgatactg gcggccgtgg
     3481 cgcaacgcct cgaaagcgcc ggtgccgacg cgcttctgat ctgtgccaac acgatgcatc
     3541 gcgtcgcccc ggcgatcgag gctgcggtga acatcccgct tcttcatctc gccgacatca
     3601 ccgcggatgc cttgcggcgg gatggatttc ggcgcgcggc gctcctcggc acgcgtttca
     3661 cgatggcgca gcccttctac cgcgagcgtc tcgaaagtca cggtttggcg attgacgtac
     3721 ccaacgcgga cgagcaggaa gacattcacc ggatcatcta cgaggaactc gtgaagggac
     3781 gcttcgaacc gcgttcgcgc gaacgcttcc tcgcgatcat gaaacgaatg catgcgaccg
     3841 gatcgcaggc cgtcatcctc ggctgcaccg agttcggtct cttgatccgc cccgaagatg
     3901 cgcccgaaat accgcggttc gacaccaccg aactccattg ccgcgccgcc gcgaattgga
     3961 tgctcgcctg acgaatgaaa aataacggca gagcgggcag cgcgagaaaa gtcctgtccg
     4021 tcatgccggc gaaggccggt atccagtgac agaagaatcc cggtctgccg gcgctccctc
     4081 cggttcgtca ttcccgcgga tgcgggaatc tggtgctgaa ataggttacc cgcaggagag
     4141 gcggcgcgac agttccgctc ctacggttgg cctgattcgg ttcgcagcac ttcgagttct
     4201 tcccagcggg cataggcgtc ggcaagtgcc tgctcggact gcgcgagccg gttctgcgtc
     4261 tccttgattt cgtcgccgtg cc
//
LOCUS       DASUJG010000346         2911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_709549, whole genome shotgun sequence.
ACCESSION   DASUJG010000346 DASUJG010000000
VERSION     DASUJG010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709549"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..1153
                     /locus_tag="VFX38_07500"
     CDS             110..1153
                     /locus_tag="VFX38_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5314728.1"
                     /translation="MNDSLSHAVLAIPDAPPAPGEARAVAPGISWLRMPLPFALDHIN
                     LWLIEERDGYTLVDCGYGDAATRAQWERHFETTLATRPIRRIVVTHCHPDHLGNAAWL
                     AARFGCIVEMTQGEYLAAHALIAEVASYAPSDVLALFARHGMALDHVKALGAHGNRYR
                     RGVPEAPPAFARLIDGDRITLGGQAFRVIAGYGHSTEHAALACVESRVLIAGDMLVPK
                     ISTNVAVWPGEPEADPVARFLDSIAAFCALPPDTLVLPSHGPPFRGIAARVAQLRAHH
                     AERLAELSAWLVQAAAPQSAHDVIPVLFRRTLDVHQRFFAMGEAIAHLNHLWRDGRAT
                     RLLVDDGAIRFTA"
     gene            1201..>2911
                     /gene="phaC"
                     /locus_tag="VFX38_07505"
     CDS             1201..>2911
                     /gene="phaC"
                     /locus_tag="VFX38_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEX5314729.1"
                     /translation="MSTPPKPATAGETPGTPAFDPVKLAESLASAADKSAKLIGEFAA
                     RNAGRQAAVASDELGLGKAFMELAANMLANPARMAEGQMRLWRDYMNLWQTSMLRMIG
                     GQALPVVEPAQGDKRFKDACWHEHFLFDYIKQSYLITARWLHEQVASVEGLSEPAKKK
                     VRFFTRQYIDALAPSNFALTNPEVFRETVASGGQNLVRGLNNLLDDIERGNGQLRISM
                     TDPKAFELGVNIATTPGKVVYQNDLMQLIQYEPTTAKTWKRPLLIMPPWINKYYILDL
                     REKNSFIRYAVNEGMTVFVVSWVNPDAKLAHKDFEDYLIDGPLAALDAIRDATGEADA
                     NVIGYCLGGTLLAATLGYLAAKKQRRIASATFMTALVDFTAAGELEVFIDEDQVASLE
                     KKMQERGYLEGNEMANTFNLLRANDLIWSFVINNYLLGRDPFPFDLLHWNCDSTRMPA
                     KMHSYYLRNMYMGNKLREPNALKLAGVPIDLSKIDVPTYFVSAIEDHIAPWKTTYAGP
                     GLMKGSKRFVLSGSGHIAGMINPPSANKYGYWTNEKLVDDADAWFAGATQHTGSWWSD
                     WRAW"
BASE COUNT          527 a          968 c          942 g          474 t
ORIGIN      
        1 gcgcgtaccc ggcggcgcgg catggcggtt cgtggcgggc atgcgaagcg agaagcgcgg
       61 cgcggcacga gccagagggc cgcggctgga ggcacgatac catatcgaga tgaacgattc
      121 gctttcgcac gcggtactcg cgatccccga tgcgccgccc gctcccggcg aggcgcgcgc
      181 cgtcgcgccc ggcatctcgt ggctgcgcat gccgctgccg ttcgcgctcg accacatcaa
      241 cctgtggctg atcgaagagc gcgatggata tacgctggtc gattgcggct acggcgatgc
      301 cgcaacgcgc gcgcagtggg agcgccattt cgagacgacg ctcgccacgc gtccgatacg
      361 ccggatcgtc gtcacgcact gtcatcccga tcatctcggc aacgccgcgt ggctcgccgc
      421 gcgcttcggc tgcatcgtcg aaatgacgca gggcgaatat ctcgcggcgc atgcgttgat
      481 tgccgaggtc gcgagctacg cgccgtccga cgtgctggcg ctcttcgcac ggcatggcat
      541 ggcgctcgac catgtgaaag cgctcggcgc acacggcaac cggtatcgtc gcggcgtgcc
      601 cgaagcgccg ccggcgttcg cgcggctgat cgacggcgac cgcatcacgc tcggcggtca
      661 agcctttcgc gtcattgcgg gctacggcca ttcgaccgag cacgcagcgc tcgcgtgcgt
      721 cgaatcgcgc gtgctgatcg ccggcgacat gctggtgccg aagatcagca cgaacgtcgc
      781 cgtatggccc ggcgagccgg aggccgatcc cgtcgcgcgc tttctcgatt cgatcgctgc
      841 gttctgcgcg ctcccgcccg atacgctggt gttgccgtcg catggtccgc cgtttcgcgg
      901 catcgccgcc cgcgtcgcgc aactgcgcgc gcaccatgcc gagcggcttg cggagctttc
      961 tgcctggctc gtgcaggcgg ccgcacctca gtcggcacac gacgtgatac cggtgctgtt
     1021 ccgccgcacg ctcgacgtgc atcagcgatt cttcgcgatg ggagaggcga tcgcgcatct
     1081 caaccatctg tggcgcgacg gacgtgcgac gcgccttctc gtcgatgacg gtgcgatccg
     1141 ctttacggcg taacattcgc gtccgcatcc gtgcaacggc ctccaacgac gattcgaaag
     1201 atgtccacgc cacccaagcc tgcaaccgcc ggcgaaacgc ccggcacgcc cgccttcgat
     1261 ccggtcaagc tcgccgaatc gctcgcctcg gccgccgaca agagcgcgaa gctgatcggc
     1321 gagttcgctg cgcgcaatgc cggcaggcag gcagcggtcg cgagcgacga gctcggcttg
     1381 ggcaaggcgt tcatggagct cgcggcgaac atgctcgcca acccggcgcg catggccgaa
     1441 ggccagatgc gcctttggcg cgattacatg aacctgtggc aaacgtcgat gctgcggatg
     1501 atcggcggcc aggcgcttcc cgtcgtcgag ccggcacagg gtgacaagcg cttcaaggac
     1561 gcgtgctggc acgagcactt cctgttcgac tacatcaagc agtcgtacct gatcaccgca
     1621 cgctggctgc acgagcaggt cgcgagcgtc gaaggactct ccgaaccggc gaagaagaag
     1681 gtccgcttct tcacgcggca gtacatcgac gcgctcgcgc cgtcgaactt cgcattgacc
     1741 aacccggaag tgttccgcga gacggtcgcg agcggcggcc agaacctggt gcggggcttg
     1801 aacaacctgc tcgacgacat cgagcgcggc aacggccagc tgcggatctc gatgaccgat
     1861 ccgaaagcct tcgagctcgg cgtcaacatc gcaacgacac ccggcaaggt cgtctaccag
     1921 aacgacctga tgcagctgat ccagtacgag ccgacgacgg caaagacatg gaagcggccg
     1981 cttctgatca tgccgccgtg gatcaacaag tactacatcc tcgacctgcg cgaaaagaac
     2041 tcgttcatcc gttatgcggt gaacgagggg atgaccgtgt tcgtcgtgtc gtgggtcaat
     2101 cccgacgcga agctcgcgca caaggacttc gaggattacc tgatcgacgg gccactcgcg
     2161 gcgctcgacg cgatccgcga cgcgaccggc gaagccgacg cgaacgtgat cggctactgc
     2221 ctcggcggca cgctcctcgc cgcgacgctc ggctacctcg cggcgaagaa gcaaaggcgc
     2281 atcgcgagcg cgaccttcat gacggcgctc gtcgacttca ccgcggcggg cgagctcgag
     2341 gtgttcatcg acgaggacca ggtcgcgtcg ctcgagaaga agatgcagga gcgcggctat
     2401 ctcgaaggca acgagatggc gaacacgttc aacctgcttc gcgcgaacga tctcatctgg
     2461 tcgttcgtca tcaacaacta cctgctcggc cgcgatccgt tcccgttcga cctgctgcac
     2521 tggaactgcg attcgacgcg gatgccggcg aagatgcaca gctactacct gcgcaacatg
     2581 tacatgggca acaagctgcg cgagccgaac gcattgaagc tcgccggcgt gccgatcgat
     2641 ttgtcgaaga tcgacgtccc gacgtacttc gtgtcggcga tcgaggatca catcgcgccg
     2701 tggaagacga cgtacgcggg gccgggcctg atgaagggca gcaagcgttt cgtcctctcg
     2761 gggtcgggcc acatcgccgg catgatcaat ccgccgagcg cgaacaagta cggctactgg
     2821 accaacgaga agctcgtcga cgatgccgat gcgtggttcg cgggcgcgac gcagcacacg
     2881 ggctcctggt ggtccgactg gcgcgcatgg a
//
LOCUS       DASUJG010000347         2223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_831220, whole genome shotgun sequence.
ACCESSION   DASUJG010000347 DASUJG010000000
VERSION     DASUJG010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831220"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1335
                     /locus_tag="VFX38_07510"
     CDS             <1..1335
                     /locus_tag="VFX38_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314730.1"
                     /translation="GTRWRMATFYVVVLLIPTVLGLWWMLPGVFSPHAYATREFDLNQ
                     RILTEGRVMVYYLRMVIAPWLPAMSLYHDGFTISTGLFKPATTIPAWALIAALLALAA
                     WLRRRLPLVALGIVWFFAAQVLESSVWPLEIAYEHRVYVADWGIVLAVAALTLLRARN
                     WRTRELVTAAAALAIVALALTTAWRAYDWRSNVALARVEASTHPHSPRATYLLSRIYT
                     NLALEGKDKYLPLAYEASEKAARVHDAGLDPWVAMVLLAAQTGGDVRPEWFDGMVKAV
                     GERPFTVSDVNALEALVGCVTRKQCVISRHQIERLFRAIDASPRLSRTGMNYANVLVT
                     EANFIGYDTPAERTRSGPLLLKAANVERDVAQYQENVFNIALADHQVEVARNMLARVE
                     KLNKLGKLDLVIADMQRRLAAAEAEPAPRPKRAQAPATHAPAAASSRPEPRR"
     gene            1402..>2223
                     /locus_tag="VFX38_07515"
     CDS             1402..>2223
                     /locus_tag="VFX38_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5314731.1"
                     /translation="MSDPRSLSFVLPAHNEANTLPALISDLRREHPAAEIIVVDDGSD
                     DGSGARATEAGADRVVRHPYPMGNGAAVKDGARAASGEVVVFMDADGQHRAEDTHKLI
                     AGLADGYVMVVGTRSGGAHANLGRRIANGVYNRLASWVTGQRIADLTSGFRAVRRKRF
                     MEILFLLPNGFSYPTTSTMAFFRAGYPVRFVPVDVRARAGAASHIRPLRDGGRFLLII
                     LKVTTLYSPLKVFTPAALLFFCIGLGYYLYTFFTSHRFTQMSALLFIISGLVFLMG"
BASE COUNT          326 a          813 c          699 g          385 t
ORIGIN      
        1 ggcacgcgct ggcgcatggc gaccttctac gtcgtcgtgc tcctcatccc gacggtgctc
       61 ggcctctggt ggatgcttcc gggagtgttc tcgccgcacg cctacgcgac ccgcgagttc
      121 gatctcaacc agcgcatcct caccgaaggg cgggtgatgg tgtattacct gcgcatggtg
      181 atcgcgccgt ggctgccggc gatgtcgctc tatcacgacg gcttcacaat ctcgaccgga
      241 ctgttcaagc cggcgaccac gattcccgcc tgggcgctga tcgctgccct gctcgcgctc
      301 gccgcgtggc tgcgccgccg cctgccgctg gtcgcgctcg gaatcgtctg gttcttcgcc
      361 gcgcaggtgc tcgaatcgag cgtctggccg ctcgaaatcg cctacgaaca tcgggtctac
      421 gtcgccgact ggggaatcgt cctcgcggtc gccgcactca cgctgctgcg cgcgcgcaac
      481 tggcgcaccc gcgaactcgt caccgccgcc gccgcgctcg cgatcgtcgc gctcgcgctc
      541 accaccgcgt ggcgggcgta tgactggcgc agcaacgtcg cgctcgcccg cgtcgaggcg
      601 agcactcacc cgcattctcc gcgcgcgacc tatctgcttt cgcgcatcta taccaatctc
      661 gcgctcgagg gcaaggacaa atacctgccg ctcgcctatg aggcctcgga gaaggcggcc
      721 cgggtccacg acgccggcct cgatccctgg gtggcgatgg tgctgctcgc cgcgcagacc
      781 ggcggcgacg tgcggcccga gtggttcgac ggcatggtca aggccgtcgg cgagcgtccg
      841 ttcacggtct ccgacgtgaa tgcgctcgaa gccctcgtcg gctgcgtcac ccgcaagcag
      901 tgcgtcattt cgcgtcacca gatcgagcgc ctcttccgcg ccatcgacgc gagcccgcgg
      961 ctttcgcgca ccggcatgaa ctacgccaac gtgctcgtga ccgaggccaa cttcatcggc
     1021 tacgacacgc ccgcggagcg cacacgcagc ggcccgctgc tcctcaaggc cgcgaatgtc
     1081 gagcgcgacg tcgcgcagta tcaggaaaac gtgttcaaca tcgcgctcgc ggatcatcag
     1141 gtcgaggttg cgagaaacat gctcgcgcgg gtcgagaaac tcaacaaact cggcaagctc
     1201 gatctcgtga tcgccgacat gcagcgccgg ctcgcagccg ccgaagccga acccgcgccg
     1261 cggccaaagc gagcccaggc gccggcgacc catgcgccgg ccgcggcttc ctcccgcccc
     1321 gagccgcgac gctgagcgcg cgcgggcttg acgctcgcgc cgtcctgccg taacgttgcg
     1381 cggactttcg ccggtgcccc cgtgagcgat ccccgatccc tttccttcgt gctgcccgcg
     1441 cacaacgagg cgaataccct gccggcgctc atttcggatc tgcgccgcga gcatcccgcc
     1501 gccgagatca tcgtcgtgga cgacggctcg gacgacggca gcggcgcgcg cgccacggaa
     1561 gccggagcgg accgcgtcgt gcgtcatccc tatccgatgg gaaacggcgc cgcggtcaag
     1621 gacggtgcgc gcgcggcgag cggcgaggtg gtcgtgttca tggatgccga cggccagcat
     1681 cgcgccgaag acacgcacaa gctgatcgcg ggcctcgcgg acggctacgt catggtcgtc
     1741 ggcacgcgca gcgggggcgc ccatgcgaac ctcgggcggc gcatcgccaa cggcgtttac
     1801 aaccgcctcg cgagctgggt caccggccag cgcattgcgg atctcacctc gggctttcgc
     1861 gccgtgcgcc gcaaacgctt catggagatt ctctttctcc tgccgaacgg cttttcctat
     1921 ccgacgacga gcaccatggc gttcttccgc gccggctatc cggtgcgctt cgtgccggtg
     1981 gacgtgcgcg cgcgcgccgg cgccgcgagt cacatccgcc cgcttcgaga cggcgggcgc
     2041 ttcctgctca tcatcctcaa ggtgacgacg ctctactcgc cgctcaaggt attcacgccg
     2101 gcggcgctgc tgttcttctg catcggcctc ggctattacc tctacacgtt tttcacctcg
     2161 catcgcttca cgcagatgag cgcgctcctc ttcatcatct cgggtctggt gttcctgatg
     2221 gga
//
LOCUS       DASUJG010000348         4725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1444380, whole genome shotgun sequence.
ACCESSION   DASUJG010000348 DASUJG010000000
VERSION     DASUJG010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4725
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1444380"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /gene="glyS"
                     /locus_tag="VFX38_07520"
     CDS             complement(<1..1088)
                     /gene="glyS"
                     /locus_tag="VFX38_07520"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEX5314732.1"
                     /translation="MSGSPARDLLIEIGTEELPAAAQLPLATALADALGEALDQCGLA
                     PASLRPFAAPRRLAVLAQAVPSREPPREIERRGPNLRAAFHPDGTPTAAAIGFARSVG
                     VKITELVRQSAADGERLVYRRHEAGRPLDAVLLAILPELFATLPLPQRMRWGSGEASF
                     LRPVRWLTLRYGRSSIPLEAFGLRATGKSRGHRLHHPGPVAFATPASYEEALQAAKVL
                     ADPTRREAELRRRVMAVLEKGDRVAAPPPGLYEEITGLVEWPVALTGRFDERYLTLPD
                     AVLVTTLAHHQRLIPVRDGDGHLQPEFVAVVDIESSDPDTIRQGLERVVRSRLEDAAF
                     YYRRDRERALGDYLADLEGLAFGRKLGN"
     gene            complement(1085..1990)
                     /locus_tag="VFX38_07525"
     CDS             complement(1085..1990)
                     /locus_tag="VFX38_07525"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014704746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEX5314733.1"
                     /translation="MPFYTRIFFGASGMPQSFQELILRLKRYWAGAGAALLEPYDMPM
                     GAATFHPATFLRALGPRPWSAAFVQPCRRPTDGRYGENPNRGQQYFQFQVVMKPSPDD
                     LQARYLGSLEALGLDLAKHEVRFVEDDWASPTLGAWGLGWEVWLNGMEISQFTYFQQV
                     GGLECRPVTGEITYGLERLALYLQDAASMFELRWQDGAGGELRYGDLYLENEREQSAY
                     NFELADVSALTVRFDAAEREHARLLEAGKVLPAYDCVLEASHTFNLLEARRALSVSER
                     QRRMLRVRRMATAVASAWTERETAA"
     gene            2094..3503
                     /gene="glnA"
                     /locus_tag="VFX38_07530"
     CDS             2094..3503
                     /gene="glnA"
                     /locus_tag="VFX38_07530"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX5314734.1"
                     /translation="MTIDEILRHVAAGEFAFVDLRFTDTRGKEQHVTVNGPVVDAAFF
                     DEGKPFDGSSIAGWKHINDSDMVLMPDPASAVRDPFAAHPTLILRCDVLEPATLEGYG
                     RDPRSIAKRAEAYILSSGLAEGAWFGPENEFFIFDDVRWGTSIDGAFYKVDSKEANWN
                     SGRVYADGNFGHRPSLKGGYFPVPPVDSLQDVRSEMCRVLGEMGIAAEVHHHEVATAG
                     QCEIGVNKNTLVRKADEVQMLKYVVMNVAQRYGKTATFMPKPLVGDNGNGMHVHVSLF
                     RKGENLFAGDGYGGLSDTALYFIGGVIRHARALNAFTNPATNSYKRLVPGFEAPVKLA
                     YSVRNRSASIRVPYAPSGKARRIEVRFPDGLANPYFAFSALALAGLDGLANRVHPGEA
                     MDKDLYDLPPEISKNVPEVAYSLEEALKALDCDREFLTAAGVFTDDTLDAYIALKGQD
                     IQRLRMTTHPVEFDLYYSL"
     gene            3508..4491
                     /gene="hemH"
                     /locus_tag="VFX38_07535"
     CDS             3508..4491
                     /gene="hemH"
                     /locus_tag="VFX38_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018294173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX5314735.1"
                     /translation="MPVGVLLVNLGTPDAPDSGSVRRFLAEFLADPRVIELPAALRWM
                     LLHGVVLRLRPRRTARAYRRIWREDGSPLAVFSGALCARLREVLAERTSRPITLRLAM
                     RYGNPGLREAFDGLVAEGVSCLIVLPLYPQYSAATTASAFDAFARAASHSRRLPSVHF
                     VSHYHDDPRYLAAVAASVSEAGERDERLLFSFHGMPQATRVAGDPYYDQCQGSARLIA
                     QSLGLAPDQWAVAFQSRFGRAAWLSPATDAVLRAWAREGVKSVSVVCPGFAVDCLETL
                     EEVALRYREVFLSAGGERFHYIPALNDRPDHAHALAELILRELERLPASEA"
     gene            complement(4505..>4725)
                     /locus_tag="VFX38_07540"
     CDS             complement(4505..>4725)
                     /locus_tag="VFX38_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314736.1"
                     /translation="LWIPLDEYLATMLRDPGLRQGLRQAALQWIHDLVRELDAARTRY
                     SAEIARGCAFAHRLWGRLRIEHAPGEKP"
BASE COUNT          782 a         1588 c         1526 g          829 t
ORIGIN      
        1 atgttgccga gcttgcggcc gaaggcgagg ccctcgagat ccgcgagata gtcgcccaga
       61 gcacgttcgc gatcccgccg gtagtaaaac gccgcatctt ccaggcgcga ccgtacgacg
      121 cgctccaggc cctgtcggat cgtgtcggga tccgagcttt cgatatcgac gacggccacg
      181 aactcgggct gaaggtgccc gtcgccatcg cgcaccggaa tcaagcgctg atggtgcgcc
      241 aaggtcgtca cgagcacggc gtcaggaagc gtgaggtagc gctcgtcaaa acgacccgtc
      301 aaagcgaccg gccactccac caggcccgtg atttcctcgt agagccccgg cggcggcgcg
      361 gcaacgcggt cacctttttc gagcacggcc attacccgtc gacgcagctc cgcctcacgc
      421 cgggtcggat ccgcgagaac cttcgccgcc tgcagcgcct cttcgtagga cgccggcgtg
      481 gcgaaggcga cggggcccgg atgatgcagc cgatgcccgc ggcttttgcc ggtcgcccga
      541 agcccgaagg cctccagagg aatgctgctt ctcccgtagc gcagcgtcag ccagcggacc
      601 ggccggagaa aactcgcctc gccgcttccc cagcgcatcc gttgcggcaa cgggagtgtc
      661 gcgaagagtt ccgggagaat ggcgagcagc accgcgtcaa gagggcgtcc ggcctcgtgg
      721 cgccgataga cgagccgttc gccgtcggca gcactctgcc gaacgagctc cgtgatcttc
      781 acgcccacgc tgcgcgcgaa gccgatcgcg gctgccgtcg gcgtgccatc gggatggaag
      841 gcggcgcgca ggttcgggcc gcggcgttcg atctccctgg ggggttcgcg gctcggcacc
      901 gcttgcgcca gcacggcgag acgccgtggc gccgcaaagg ggcgcagact cgcgggcgcg
      961 agcccgcact gatcgagcgc ctcgccgagc gcgtcggcca gcgccgtggc tagtggcagc
     1021 tgcgccgcgg cgggcagctc ttccgtcccg atttcgatca ggaggtcgcg cgccgggctg
     1081 ccgctcatgc cgcggtttcg cgttcggtcc aggcggaggc gacggccgtt gccatgcgtc
     1141 gtacccgcag catccgccgc tgtctctcgg agacggacag cgcccgccgt gcctcgagca
     1201 ggttgaacgt gtgcgaggct tccagcacgc aatcgtaagc cggcaggacc ttccccgcct
     1261 cgagaagacg cgcatgttcg cgctcggcgg cgtcgaagcg caccgtgagt gcggacacat
     1321 ccgcaagttc gaaattgtag gcggattgct cgcgctcgtt ttcgagatag agatccccgt
     1381 agcggagttc gccgccggcc ccgtcctgcc accgcagttc gaacatgctc gcggcgtcct
     1441 gcagatagag tgcgaggcgc tccagcccgt aggtaatctc gccggtgacc ggacggcatt
     1501 cgaggccgcc gacctgctgg aagtaggtga actggctgat ctccatcccg ttcagccaca
     1561 cctcccagcc gaggccccag gcacccaggg ttggcgacgc ccagtcgtcc tcgacaaagc
     1621 gcacctcgtg cttcgcgaga tcgaggccga gcgcctcgag cgagccgaga tagcgtgcct
     1681 gcaggtcatc gggtgaaggt ttcatcacca cctgaaactg gaaatactgc tgcccgcgat
     1741 tgggattctc gccatagcgc ccgtcggtcg ggcgccggca gggctgcacg aaggcggcgc
     1801 tccacggccg ggggccgagc gcccgcagga aggtcgccgg atgaaacgtc gccgcgccca
     1861 tcggcatgtc gtagggttcg agcaacgcgg cgcccgcccc ggcccagtat cgcttgagcc
     1921 ggagaatgag ttcctgaaag ctttgcggca tgccggaagc cccgaagaag atcctagtat
     1981 aaaacggcat ataattttcg ccgtcgcgag gcgacccgct tgcgtcgccg agcgatgcgc
     2041 cgtggttgcc tggtgatgaa ttgctgacgg gcccgccttc cttggagaac gtcatgacaa
     2101 tcgacgaaat cctgcgccat gtggccgcgg gcgagttcgc gttcgtggac ctgcgcttca
     2161 ccgacacgcg tggcaaggaa caacacgtca ccgtcaacgg tcccgtggtc gatgcggcat
     2221 tcttcgacga aggcaaaccc ttcgacggct cgtcgattgc gggctggaag catatcaacg
     2281 attccgacat ggtgctcatg cccgatcctg cgagcgcggt gcgcgatccc tttgcggcgc
     2341 atccgacgct gatcctgcgc tgcgacgtgc tggagccggc gaccctcgaa ggctacggtc
     2401 gcgatccgcg ctcgatcgcc aagcgcgccg aggcctacat cctctcgagc gggctcgccg
     2461 aaggtgcctg gttcggaccg gaaaacgaat ttttcatttt cgacgacgtg cgctggggaa
     2521 cgtcgatcga cggtgcgttc tacaaggtcg attcgaaaga ggcgaattgg aactccgggc
     2581 gcgtgtacgc cgacggcaac ttcggacacc gcccttcgct caagggcggc tattttccgg
     2641 tgccgcccgt ggattcactg caggacgtgc gctcggaaat gtgtcgggtg ctgggcgaga
     2701 tgggcattgc cgccgaggtt catcatcacg aggtcgcgac ggccggtcag tgcgagatcg
     2761 gagtgaacaa gaacaccctg gtgcgcaagg ccgacgaggt gcagatgctg aagtatgtgg
     2821 tcatgaacgt cgcgcagcgc tacggcaaga ccgcaacctt catgcccaag ccgctcgtcg
     2881 gcgacaatgg caacggcatg catgtgcacg tgtcgctctt ccgcaagggg gaaaatctct
     2941 tcgccggcga tggctacggc gggctgtccg acaccgccct ctatttcatc ggcggcgtca
     3001 tccgccacgc gcgcgctctc aacgccttca ccaacccggc gaccaacagc tacaagcgtc
     3061 tcgtgcccgg cttcgaggcg ccggtgaagc ttgcctactc ggtgcgcaat cgctcggcct
     3121 cgatccgggt gccctatgcg ccgagcggaa aagcgcggcg catcgaggtt cgatttcccg
     3181 atgggctggc caatccctac tttgccttca gcgctctcgc gctcgccggc cttgacggcc
     3241 tcgccaaccg cgtgcatccg ggcgaggcga tggacaagga cctttacgat ctgccgccgg
     3301 aaatatcgaa gaacgtcccc gaggtcgcct attcgctcga ggaggcgctc aaggcgcttg
     3361 actgcgatcg cgagtttctt accgcagcgg gagtatttac cgatgatacg ctcgatgcct
     3421 acatcgcctt gaaggggcag gacattcagc gcctgcggat gaccacgcat ccggtcgagt
     3481 ttgatctcta ctacagcctg tagcggcgtg ccggtcggag tcctgctcgt caacctcggc
     3541 acccccgacg ctcccgattc gggttcggtg cgccgattcc tcgccgagtt cctcgccgat
     3601 ccgcgcgtga tcgaattgcc tgccgccttg cgctggatgc tgctgcacgg cgtcgtgctg
     3661 cgtctgcgcc cgcggcgcac cgcccgcgcc taccgccgca tctggcgtga ggacggctcg
     3721 ccgctcgccg tgttcagcgg cgctctgtgc gcgcggctgc gagaggtcct cgccgaacga
     3781 acatcacgcc cgattacgct acggctcgca atgcgctacg ggaatcccgg tctgcgcgag
     3841 gcgttcgacg gtctcgtggc agaaggcgtc tcgtgcctga tcgtgctgcc tctttatccg
     3901 cagtactcgg ctgcgaccac cgcttcggca ttcgatgcat tcgcccgggc cgcgtcgcat
     3961 tcgcgccgtc ttccttccgt gcatttcgtg agtcactatc acgacgatcc ccgctatctc
     4021 gctgccgtcg cggcgtccgt gtcggaagcc ggcgagcgcg acgagcgcct gcttttctcc
     4081 tttcacggca tgccgcaggc gacccgcgtc gcgggcgatc cctactatga tcagtgtcag
     4141 ggaagcgcgc ggctcattgc gcaatctctc ggactcgcgc ccgatcaatg ggcggtcgct
     4201 tttcagtcgc gcttcggccg cgccgcgtgg ctgtcgccag cgaccgacgc ggtccttcgc
     4261 gcctgggcgc gcgaaggcgt gaagtcggtg agcgtcgtgt gtcccggctt cgccgtggat
     4321 tgcctggaaa cgctggaaga ggtcgccctt cgctaccggg aggtattcct ctcggcgggc
     4381 ggcgagcgct ttcattacat cccggcgctc aacgatcgtc ccgatcacgc acacgcgctc
     4441 gcagaactga tactgcggga gctggaacgc ctgcccgcct ccgaggccta gagcggatca
     4501 cggttcaagg cttctcgccg ggagcatgct cgatgcgcaa gcgaccccac aagcggtgcg
     4561 caaaggcgca ccccctggcg atctcggcgg agtaacgcgt ccttgccgca tcgagttccc
     4621 gcacgagatc gtgaatccat tgcaatgccg cctgccgcaa cccctgccgc aggccgggat
     4681 cgcggagcat cgtagcgaga tattcgtcga gaggaatcca cagca
//
LOCUS       DASUJG010000349         2087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1566998, whole genome shotgun sequence.
ACCESSION   DASUJG010000349 DASUJG010000000
VERSION     DASUJG010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1566998"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1859
                     /locus_tag="VFX38_07545"
     CDS             <1..1859
                     /locus_tag="VFX38_07545"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5314737.1"
                     /translation="IVMVEAIHRGHSTGMPTDAAVESALAELSRPLIASTLTVIAVFM
                     PLIFVSGITGLFFRALAITLGGGLAISLGLAIFFTPALEELIGRWRRKSRPPGRVFGA
                     MRSAFLAELRPFIRFPALALAVAAACFIAAFAFYHAVGTDYLPALDEGAFTLDYITPP
                     PSTLADTDALLGKIEDILRATPEVAAFSRRTGTQLGFFLTESDRGDFSVLLKRQRSRS
                     IDEIMNDVRRRIVAAVPGVHIEFSQVLQDLIGDLSGVPQPIEINVFGADQASIEATAR
                     RVATEIGGVPGVVDVFDGIVLSSPEEQIVINQSAAARYGLSADAIRATLETAIEGTVA
                     TQLSIGDRLIGVRVRYPPSFHRDLPALSEVQLRTPSGSTVPLSAVAELRWGGETTELA
                     RERLRPVVHVTARLEGVDLGTALGQIRPRLAKMVLPAGVSLELGGLYEQQQQAFHQLS
                     LVMAAGLVMVLLIVLWEFGRLAPALATVIAALACLVGSFAALGLTGITLNISSFMGVI
                     MVAGITAKNGIMLLDRAEHHVAEGIAPAKALADAAIVRLRPIVMTTLATAAGLLPLAL
                     GYGAGAKVQQPLAVAVIGGLVLAMLLSSAIAGGIYLLGTRPAPPGRTESENP"
     gene            1961..>2087
                     /locus_tag="VFX38_07550"
     CDS             1961..>2087
                     /locus_tag="VFX38_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011612874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5314738.1"
                     /translation="METAMPTQQGKQRLKGKAAIVTGAGRGIGRAEAMLLAAEGAS"
BASE COUNT          320 a          696 c          691 g          380 t
ORIGIN      
        1 cgatcgtcat ggtcgaagcg atccatcgcg gacattcgac tgggatgccc accgacgccg
       61 cagtcgagag cgcgctcgcc gaactcagcc gcccgctcat cgcttcgacg ctcaccgtga
      121 tcgcggtctt catgccgctg atcttcgttt cgggcatcac cgggctgttt tttcgtgcgc
      181 tcgcgatcac gctgggcggc ggccttgcga tttcgctcgg ccttgcgatt ttcttcacgc
      241 ccgcgctgga agagttgatc ggacgctggc ggcgcaagag ccgtccgccg ggacgggtgt
      301 tcggcgcgat gcgctccgcc tttctcgccg agctgcggcc gttcatccgc tttccggcgc
      361 tggcgctcgc cgtcgccgcg gcctgcttca tcgcggcttt cgcgttttac cacgcggtgg
      421 gcacggacta tctgccggcg ttggacgagg gcgccttcac gctcgactac atcactccgc
      481 cgcccagcac gctcgccgat accgatgcgc tgctgggtaa aatagaggac attctccggg
      541 cgacgcccga ggtggcagcc ttctcgcggc gcaccggcac gcaattggga ttcttcctta
      601 ccgaatcgga ccgcggtgac ttctccgtgc tcctcaagcg gcaacgctcg cgatcgatcg
      661 acgagatcat gaacgacgtg cgcaggcgca ttgtggccgc ggttcccggc gtgcatatcg
      721 agttctcgca ggtgttgcag gacttgatcg gcgacctttc gggcgtgccc cagccgatcg
      781 agattaacgt attcggcgcc gaccaggctt cgatcgaagc gaccgcgcgc cgcgtcgcca
      841 ccgagatcgg cggcgtcccg ggagtcgtcg acgtcttcga cggcatcgtg ctgagcagcc
      901 ccgaggagca gattgtgatc aaccagagcg ccgccgcgcg ctacggactg agcgccgacg
      961 ccatccgcgc caccctcgaa actgcgatcg aaggtaccgt cgcgacacaa ctcagcatcg
     1021 gcgaccggct catcggcgtg cgcgtgcgct atccgccgag cttccatcgc gatctgcccg
     1081 cgctctccga agtgcagctg cgcacgccgt caggcagcac tgttccactg agcgcggtgg
     1141 ctgagctgcg ctggggaggc gagaccaccg agcttgcccg cgagcgcttg cggcccgtgg
     1201 ttcacgtcac ggcgcgtctt gagggcgtcg acctgggcac cgcgctcggc cagattaggc
     1261 cgcgtctcgc gaagatggtg ctgcccgcgg gtgtgtcgct ggaacttggc gggctctatg
     1321 aacagcaaca gcaggcgttt catcaacttt cgctggtgat ggcggcgggg ctggtgatgg
     1381 tgctgctgat cgtattgtgg gaattcggac ggctggcccc tgccctggcc acggttatcg
     1441 cggcgctggc atgcctcgtc ggcagcttcg ccgcgctggg acttaccggg atcacgctca
     1501 acatctcgtc gttcatgggc gtcatcatgg tcgctggcat caccgctaaa aacggaatca
     1561 tgctgctgga ccgcgccgag caccacgtcg ctgagggtat cgcaccggcc aaggcgctgg
     1621 ccgacgcggc gatagtgcgg ctccggccga tcgtgatgac cacgctggcg acggcagcgg
     1681 gcctgctgcc gctcgcgctc ggctacggcg cgggggccaa ggtccagcaa ccactggcgg
     1741 tggcggtgat cggcgggctg gtgctggcga tgctcctctc ctccgcgatc gccggcggaa
     1801 tctacctgct cggtacgcgc cctgctccgc ctggccgcac tgaatcggaa aatccttgat
     1861 atgcgccggc gctctcgcat ggcgcgcgat tcggcgctat tcataggcgc gagccactcg
     1921 ccgcatctgc caaccggcgg cccgcggcgc gcatcaataa atggagacag cgatgccaac
     1981 gcaacaaggg aaacagagac tcaagggaaa ggccgcgatt gtaactggtg cgggccgcgg
     2041 aatcggccgt gcggaggcga tgctgctcgc ggccgagggc gcaagcg
//
LOCUS       DASUJG010000350         3175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_587973, whole genome shotgun sequence.
ACCESSION   DASUJG010000350 DASUJG010000000
VERSION     DASUJG010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587973"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VFX38_07555"
     CDS             complement(<1..558)
                     /locus_tag="VFX38_07555"
                     /inference="COORDINATES: protein motif:HMM:NF045000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HEX5314739.1"
                     /translation="MIDSLHTLKEAGALDALDLAFGEWIAKRNTETAGFLGALVSAEV
                     AKGHVCLDLGTVESALETHDVTPPEDWRTELAQSKAIAKPGERAPLVLYGDRLYLHRY
                     WTYETRIADALRSLAAPLPGIAPELIKHGLNQLFPDIGNGELDRQALAATIGATRRLT
                     ILTGGPGTGKTWTVLRLIALALLLDP"
     gene            complement(895..>3175)
                     /locus_tag="VFX38_07560"
     CDS             complement(895..>3175)
                     /locus_tag="VFX38_07560"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX5314740.1"
                     /translation="GDPKQAIYRFRGADVFTYMRARRQCEQSDRLYGLTENYRSTGAL
                     LDAFNALFEGRDNPFEYEQIPYSHVTSGREVAALNAKGDAACTVLWHAGADAKILKDE
                     AARIAADVCATEIARLLELGASGRAHYPKDGNTQRVEAKDIAVLVDKNRQGLAVQLAL
                     RRYGIAAAMVATASVFAGEEAEELQIVLAAVAEPANGALLRRALATRLLGATASDLAA
                     WANDEPRWGAIVSNFRDYHALWQSRGFAAMFARLIAEEGVIERTLARDDGERVMTNLR
                     QLAELAGEQAAHCPGIASLLVWFARERANVAKAEDRQLRLESDENLVRIATVHKAKGL
                     EYPIVFLPFLWDGKQPDKKTANPAVLAHDEDYRSVLDLGSEHVGQRQEDAAQEARSEE
                     ARKLYVALTRAQRACYLIAMPANQLELTALARMLNVDAFEELQPKLQDWTKAAGGSVK
                     IRAPLSAGATGQLSSARPHGQAREFPFADRLRQRFFVASYSRLAEGARGASAEAPDRD
                     ETVATVPAPEQAKGIHAFPAGPASGTFMHAVIEALDFDADENAISEAVRSQSAFHGIE
                     PRWNETLTGWLLEIFATPLGAPGCALRDIRRERRIDELEFYFPLNAIRAPALDEALRQ
                     FSPQGPRPSLQFDDVQGLMKGYLDLVFEQGGRYWITDYKSNRLGSDLAAYTPQALDRA
                     MGEHRYDLQYLIYTVALHRYLATRIADYDYDRHFGGVLYLFLRGMAPVAPEPRGVWHT
                     RPEKSDVERLDAYLAGAVP"
BASE COUNT          543 a          963 c         1050 g          619 t
ORIGIN      
        1 gggatcaagc aggagcgcga gcgcgatgag ccggagcacc gtccaggtct tgccggtgcc
       61 ggggccgccg gtgagaatcg tgagacggcg cgtcgcgccg atcgttgcgg cgagcgcctg
      121 acgatcgagt tcgccgttgc cgatgtcggg gaagagttga ttcaagccgt gttttatcag
      181 ttccggcgcg attccgggaa gcggtgccgc gagggaacgc aaggcgtcgg caatgcgggt
      241 ttcgtaggtc cagtagcgat ggagatagag acggtcgccg tagagaacga gcggcgcacg
      301 ctcgccgggt tttgcgatcg ctttgctttg cgcgagttcg gtacgccagt cttccggcgg
      361 cgtgacatca tgcgtttcca aagcgctttc aaccgtgccc agatcgaggc acacgtgacc
      421 tttggcgact tcggccgaca cgagcgcgcc aagaaagccg gcagtttcgg tgtttcgctt
      481 cgctatccat tcgccgaacg cgaggtccag cgcgtccaga gcgccggctt ccttcaatgt
      541 gtgcagcgaa tcaatcatga ccggcctcaa tcgcgttttg tatcccggcc tgcggtcgcg
      601 atgattgttc gactggattc cggccttcgc cggaatgacg gatggaattc ttgcaggcgg
      661 gcacaaggcc gaaggccgtt gccggcgtgg tgtgccgaat gcccgcaacg cttcgcttat
      721 gcgcgcctgc gaagaatgca ttgacttccc ccttcgcttg cgaaggggga ttgaggggga
      781 tgccttcgcc agacgcctga agaaaccccc tccagctccc ccttccccgc aaggcgggca
      841 agggggagag ccaaacggat gcgaaatcgc gagcaagctc gctcctgcgg atgttcatgg
      901 cacggcgccc gcgaggtagg cgtcgaggcg ttcgacgtcg cttttttccg ggcgcgtgtg
      961 ccagacgccg cgcggctccg gtgcaaccgg agccatgccg cgcaggaaga ggtagagcac
     1021 gccgccgaaa tgccgatcgt agtcgtaatc ggcaatccgt gtcgcaagat agcgatggag
     1081 cgctacggtg tagatcaggt attgcaggtc gtaacgatgt tcgcccatcg cccgatcgag
     1141 cgcttgcggc gtgtaggcgg cgagatcgga gccgaggcgg ttggacttgt agtcggtgat
     1201 ccagtagcgc ccgccctgct cgaagacgag atcgagatag cccttcatca atccctgcac
     1261 gtcgtcgaat tggagtgacg ggcgcggccc ctgcggcgag aattgccgga gagcctcgtc
     1321 gagtgccggc gctcggatcg cattcaacgg aaagtaaaac tcgagttcgt ctatgcgccg
     1381 ttccctacgg atatcgcgga gcgcgcagcc gggtgctccg agtggggtcg cgaagatttc
     1441 gagaagccat ccggtcaagg tttcgttcca tcgcggttcg atgccgtgga acgcgctttg
     1501 ggatcgcacc gcttcggaaa tggcattttc atcggcatcg aagtccagcg cctcgatcac
     1561 cgcgtgcatg aaagtgccgc tcgccgggcc cgccggaaag gcgtgaattc ctttcgcctg
     1621 ctcgggcgcc ggtaccgtgg cgaccgtctc gtcccggtcc ggggcttccg cggaggcgcc
     1681 gcgtgcgcct tcggcaagtc ggctgtaact tgcgacgaaa aatcgttgcc gcaagcgatc
     1741 cgcaaacgga aattcgcgcg cttgtccatg cgggcgtgcc gacgaaagct ggcctgttgc
     1801 gccggcggag agtggcgcgc ggatcttcac cgatccgcct gcggcctttg tccaatcctg
     1861 caatttcggc tgcaactctt cgaatgcgtc aacgttcagc atacgcgcca gcgcggtcaa
     1921 ttccagttga ttcgcgggca tcgcaatcag gtaacaggcg cgctgcgcac gggtcagcgc
     1981 aacatagagc ttgcgcgcct cttcggaacg ggcttcttgg gcggcgtctt cctgacgttg
     2041 gccaacatgt tccgaaccga gatcaaggac gctgcgataa tcctcatcgt gtgcgagtac
     2101 agccggattc gccgtcttct tgtccggttg tttaccgtcc cagagaaacg gcagaaagac
     2161 gatcgggtat tcgagtccct tggccttgtg aacggtggcg atgcgaacga ggttttcgtc
     2221 gctttcgagg cgcagttgcc ggtcttccgc cttcgcgacg ttggcgcgct ccctcgcgaa
     2281 ccagacgagc agcgacgcga tgccggggca atgcgcggcc tgttcgcccg cgagttcggc
     2341 gagctgacgc agattggtca tgacgcgttc gccatcgtcg cgcgcaaggg tgcgttcgat
     2401 gacaccctct tcggcgatca gccgcgcaaa catggcagcg aaaccacggc tctgccagag
     2461 ggcgtgatag tcgcgaaagt tcgagacgat ggctccccag cgcggctcgt cgttcgccca
     2521 ggccgcgaga tcgctcgccg tcgcgccgag caggcgggtc gcaagcgccc ggcgcaggag
     2581 cgcgccgttc gccggttcgg ccacggccgc gagcacgatc tgcagttcct cggcttcttc
     2641 gcctgcaaag acacttgcgg tcgcgaccat cgcggcggcg attccgtagc ggcgaagcgc
     2701 gagttgcacc gcgagtccct ggcgattctt gtcgaccagc acggcaatgt ctttggcctc
     2761 cacgcgctga gtgttgccgt ctttcgggta atgagccctg ccgcttgcgc caagttcgag
     2821 cagccgtgcg atttccgtcg cacaaacgtc ggccgcgatc ctggcggctt cgtccttgag
     2881 aatcttcgca tccgctcccg cgtgccacag caccgtgcag gcggcgtcgc ccttcgcgtt
     2941 cagcgccgcg acctcgcggc cggacgtgac gtgggaatag gggatttgct catattcgaa
     3001 gggattgtcg cggccttcga aaagcgcgtt gaaagcgtcg agtagagcgc ctgtgctgcg
     3061 atagttttcg gtcaggccat agagacggtc cgattgttcg cattggcgcc gggcccgcat
     3121 gtaggtgaac acgtcggcgc cgcggaagcg gtagatcgcc tgcttggggt cgccg
//
LOCUS       DASUJG010000351         4229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1811189, whole genome shotgun sequence.
ACCESSION   DASUJG010000351 DASUJG010000000
VERSION     DASUJG010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1811189"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..2197)
                     /locus_tag="VFX38_07565"
     CDS             complement(47..2197)
                     /locus_tag="VFX38_07565"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HEX5314741.1"
                     /translation="MSPETHSQLANFIWSICNLLRGPYKRNEYRKVILPLTVLRRFDC
                     LLAPTKARVLAEYPKIKGKPETVVLSLLERITRRSFYNLSPMQFDKDAEHSLLDDPNH
                     LAAHLNKYINSFSPNVRAIMERFAFDQQIARMAGKNLLYEVIKAFAKIDLSPARVDNV
                     QMGYVFEELIRIGAEQANEEAGEHFTPREVIKLMVNLLLSPETDLRKSHVVKTIYDPA
                     CGTGGMLSVAEGYLRSLNDDAKPMLFGQDWNDEAWAVCKSDMLIKGEEADNIRLGDIF
                     SQDRFERDEKDEKQTFDYMLANPPFGVEWKQQEKFIRREADTFGYEGRFGAGLPRIND
                     GSLLFLQHMLSKMRAAEKGGSRIGIVFNGSPLFTGDAGSGESNIRRWIIENDWLEAVV
                     ALPDQLFYNTGISTYLWILTNRKEARRKGKVQLVDARRFFVKMPKSLGNKRNKIGDPT
                     DRAGEPDQIAEITKIFGNCKEGETRRFTEEDPITKQPVERERVVCKLFDNTDFGFHKI
                     TVERPLRLNLQATPERIARLEEESGFRKLATTNKKNEKTRLAEIAAGEARQRQIREFL
                     TAFGKSNKGKLYKDREVFLTDLRKSAKSVTLSAAELKAVLNALGERDETAEICRDRKG
                     RPEPDPELRDTENVPLKERIEAYFEREVLPHVPDAWIDESKTKAGYEIPFNRHFYVYE
                     PPRPLDVIEADIKTLEQDITSLLANLTGSKDPAR"
     gene            complement(2436..3728)
                     /locus_tag="VFX38_07570"
     CDS             complement(2436..3728)
                     /locus_tag="VFX38_07570"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX5314742.1"
                     /translation="MGKNVILVGIQWGDEGKGKLVDLLTEHARAVVRFQGGHNAGHTI
                     VVGGKKTVLHLVPSGVLRDGVRCLIGNGVVRDPAALFAELELLEGEGVPARERLGLSA
                     ACPLILPSHAALDQAREKAKGAAAIGTTGRGIGPAYEDKVARRAVRVGDLFRRERFAA
                     KLGEVLEFHNFMLKNYFHAPQTDFQETLERYLGFGETLAPLVADVAAELVTFRKDGAN
                     VLFEGAQGTLLDVDVGTYPFVTSSNTTAGAAATGTGIGPREFDSVLGVVKAYTTRVGG
                     GPFPTELTDFLGRELGRRGQEFGSTTGRARRCGWFDAVVMRRAIVNNSLSALAVTKLD
                     VLDEMETVRIAVAYRHGATEVADVPIGGEAYASAEPMYIDMPGWQSSTVGITRYTELP
                     ANARAYLEKIEELAGVPLAMISTGPDRDHTITLHAPFA"
     gene            complement(3732..3932)
                     /locus_tag="VFX38_07575"
     CDS             complement(3732..3932)
                     /locus_tag="VFX38_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HEX5314743.1"
                     /translation="MISIRVLIAAVALAIIFEGILPFVSPRHFRKGLVPMLHLGDRAL
                     RIMGLVALVLGVTLLYLARYTG"
     gene            complement(3946..>4229)
                     /locus_tag="VFX38_07580"
     CDS             complement(3946..>4229)
                     /locus_tag="VFX38_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEX5314744.1"
                     /translation="SEQAAAIRNKGEARAAAIISAANARAATIHGEGEAKLVAIYAAA
                     SGDEPQFFDFYRQLQAERRQLLTNTKLLVISVDSPWFRALGPADKKGGG"
BASE COUNT          684 a         1292 c         1383 g          870 t
ORIGIN      
        1 gtgcggccct tgccgtcgat ctcgcggggc ggacggggcg atgtgttcat cgggccggat
       61 ctttgctgcc ggtcaagttc gcaaggaggc tcgtgatgtc ctgctccagc gtcttgatgt
      121 ccgcttcgat gacgtccagc gggcgcggcg gttcatacac gtagaaatgc cggttgaacg
      181 ggatttcgta gccggctttg gtcttgcttt cgtctatcca ggcatccggc acgtgcggca
      241 gcacttcacg ctcgaaatag gcttcgattc tttccttgag cggcacgttt tcggtatcgc
      301 gcaattccgg atcgggctcc ggcctgcctt tccggtcgcg gcagatttcg gcggtttcgt
      361 cgcgctcgcc gagcgcgttg agcaccgctt tgagttcggc agcggagagg gtcacggatt
      421 tcgccgactt ccgcagatcg gtcaggaaga cttcccggtc cttgtagagc ttgcctttgt
      481 tcgacttgcc gaacgcggta agaaactcgc gaatctgccg ttggcgtgct tcgcccgccg
      541 cgatctcggc caggcgtgtt ttttcgttct tcttgttggt ggtggcgagt tttctgaagc
      601 cgctctcttc ctccagccgg gcgatgcgct cgggcgtggc ctgtaagttc aatcgcaacg
      661 ggcgctcgac ggtgattttg tggaagccga agtccgtgtt gtcgaacaac ttgcagacga
      721 cgcgctcgcg ctccaccggc tgtttggtga tcgggtcttc ttcggtgaag cgacgggttt
      781 cgccttcctt gcagttgccg aaaatcttgg tgatctcggc gatctggtcc ggctcaccgg
      841 cacgatcggt ggggtcgccg atcttgttgc gcttgttgcc gaggcttttc ggcattttga
      901 cgaagaagcg gcgcgcgtcc acgagctgga ccttgccctt ccgccgcgct tccttgcgat
      961 tggtgagaat ccacagatag gtggaaatgc cggtgttgta gaagagctga tccggcagcg
     1021 cgaccacggc ctccagccag tcgttctcga tgatccaccg gcggatgttg ctttcgccgc
     1081 tgccggcgtc gccggtgaac aacggcgagc cgttgaagac gatgccgatg cggctgccgc
     1141 ccttttcggc cgcgcgcatc ttggagagca tgtgttgcag gaacaggagc gagccatcgt
     1201 tgatgcgcgg cagcccggcg ccgaagcggc cctcgtagcc gaacgtgtcg gcctcacgcc
     1261 gaatgaactt ctcctgctgc ttccattcga ccccgaaggg gggattcgcg agcatgtaat
     1321 cgaaagtctg tttctcgtct ttttcgtcgc gctcgaagcg atcctgtgaa aagatgtctc
     1381 cgagacggat gttgtcggcc tcctcgccct tgatgagcat gtccgacttg cacaccgccc
     1441 aggcttcgtc gttccagtcc tggccgaaca gcatcggctt ggcgtcgtca ttcaggctgc
     1501 gcaggtagcc ctccgctacc gacaacatgc cgcccgtgcc gcaggccgga tcgtaaatcg
     1561 ttttgaccac gtggctcttg cgcaaatccg tttccggcga gagcagcagg ttgaccatca
     1621 gcttgatgac ctcgcgcggc gtgaagtgct cgccggcttc ctcgttcgcc tgttcggcgc
     1681 cgatgcgaat cagttcctcg aacacgtagc ccatctgcac gttgtccacg cgcgcgggcg
     1741 aaaggtcaat cttcgcgaat gccttgatta cttcatagag caggttcttc cccgccatgc
     1801 gggcaatctg ctgatcgaag gcaaaacgct ccatgatcgc gcgcacgttc ggcgagaagc
     1861 tgttgatgta cttgttcaga tgagccgcga ggtggtttgg atcgtcgagc agtgaatgct
     1921 ctgcatcctt gtcgaactgc atcggtgaaa ggttgtagaa ggaccgccgg gtgatccttt
     1981 ccagcaggct gagaacgacc gtttccggct tgcccttgat cttcgggtat tcggcgagca
     2041 ccctcgcctt ggtcggcgcg agcaggcagt cgaagcgccg cagcaccgtc agcggcagga
     2101 tgactttgcg gtattcattg cgcttgtacg gcccgcgcaa caggttgcag atgctccaga
     2161 tgaagttggc gagctgggag tgggtttcgg gactcatgcg cctatccttg agtcaattcg
     2221 atccggcatg gcccggtcgc cgcccacaag cgcgacattc ctcatggcgg aaatatccgc
     2281 ggatcgcgtc gccatacccc gaactcccct tctcacgccg cgcttgcgcc gctgccaatg
     2341 ataccgcggc gcggcccgcc gtcgcgatcg ttcgggcaaa aaggattgca accgatgcgg
     2401 ttcgctgcgc tcaccagatc ctgcgaatcc ggaacctacg cgaagggcgc gtggagggtg
     2461 atggtgtggt cgcggtcggg gccggtggag atcatggcga ggggaacgcc ggcgagttct
     2521 tcgatttttt cgagataggc gcgggcgttc gcggggagtt cggtgtaacg ggtgataccg
     2581 acggtggagc tttgccatcc cggcatgtcg atgtacatgg gttcggcgct cgcgtaggct
     2641 tctccgccga tcggcacgtc ggcgacttcg gtcgcgccgt ggcggtaggc gacggcgatg
     2701 cgcaccgttt ccatttcgtc gagcacgtcg agcttggtga cggcgagtgc gctgagactg
     2761 ttgttgacga tcgcgcggcg catgacgacg gcgtcgaacc agccgcagcg gcgcgcgcgc
     2821 cccgtcgtcg atccgaattc ctggccgcgc cggccgagtt cgcggcccag aaaatcggtg
     2881 agttcggtcg ggaacggccc gccgccgacg cgcgtggtgt aggccttgac gacgccgagc
     2941 accgaatcga attcgcgcgg gccgattccg gtgccggtcg ccgccgctcc ggccgtggtg
     3001 ttggacgaag tgacgaaggg ataggtgccg acgtcgacat cgagcaaggt gccctgcgca
     3061 ccttcgaaga gcacgttcgc accgtccttg cgaaacgtca cgagttcggc ggcgacatcg
     3121 gcgaccagcg gcgcgagcgt ctcgccgaag ccgagatagc gttcgagcgt ttcctggaag
     3181 tccgtctgcg gcgcgtggaa ataattcttg agcatgaagt tgtgaaactc gagcacctcg
     3241 ccgagcttgg cggcgaagcg ctcgcggcgg aagagatcgc cgacgcgcac ggcgcggcgt
     3301 gcgaccttgt cttcgtaggc ggggccgatg ccgcgcccgg tggtgccgat ggcggcggcg
     3361 cccttggcct tttcgcgggc ctgatcgagc gccgcgtgcg agggcaggat gagcggacag
     3421 gcggcgctca agcccagacg ttcgcgcgcg ggcacgccct cgccttcgag aagttcgagt
     3481 tcggcgaaga gcgccgcggg atcgcgcacg acgccgttac cgatgaggca gcgcacgccg
     3541 tcgcgcagca cgcccgaggg aacgagatgc aacaccgtct tcttgccgcc gacgacgatg
     3601 gtgtgcccgg cgttgtgtcc gccctgaaaa cgcacgacgg cgcgcgcgtg ctcggtgagc
     3661 agatccacga gcttgccctt gccctcgtcg ccccactgga tgccgacgag aatgacgttc
     3721 ttgcccatgt gttatcccgt gtatcttgcg aggtaaagaa gagtcacgcc gaggacgagc
     3781 gcgacgagtc ccatgatgcg cagcgcccga tcgcccagat gcagcatcgg cacgaggccc
     3841 ttgcggaagt gccgcgggct cacgaaggga aggattccct cgaagatgat cgcgagcgcg
     3901 accgcggcga tgagtacgcg aatcgaaatc atgcgggccc tgcgttcaac ctccgccttt
     3961 tttgtccgcg gggccgagcg cgcggaacca ggggctgtcc accgaaatca ccagcaactt
     4021 cgtgttcgtg agaagttgcc ggcgctcggc ctgaagctgc cgatagaagt cgaaaaactg
     4081 cggctcatcg ccggaagccg ccgcgtagat cgcgaccagt ttcgcttcgc cctcgccgtg
     4141 aatggtggcg gcgcgcgcat tggccgcgga gatgatcgcc gccgccctcg cttccccctt
     4201 gttccggatc gccgccgcct gctcgctcc
//
LOCUS       DASUJG010000352         3745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_833566, whole genome shotgun sequence.
ACCESSION   DASUJG010000352 DASUJG010000000
VERSION     DASUJG010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833566"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..813
                     /locus_tag="VFX38_07585"
     CDS             229..813
                     /locus_tag="VFX38_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314745.1"
                     /translation="MTVYSPPGFTVSIRTPVGASFGLIGGAIAAGEINEKSAEFTKAV
                     AAQGFSVQQALTEQLKADLEAEGYAVDLVPATRSGDSFLRSYPQGSGGEAWLDVVVKD
                     HSVGYRAAGNKTSYYPYVLVSAQLVGPGGGVLYSQQLVYNPIHPPKNARTIAPSGEYA
                     YANFDQLMADPARATAGLKQAVAQIAAAIAADLK"
     gene            828..1466
                     /locus_tag="VFX38_07590"
     CDS             828..1466
                     /locus_tag="VFX38_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314746.1"
                     /translation="MRRLAAAAMVAICGLLAACTQPVPTAAPLSAGYLMAGRSCGGGA
                     TLPATALTIGTRQVIHLAESSPCITAGSGVNEPAVLLSLPVSSDTYYVTVRVWRGAKI
                     VVPRIELLDGNRRPVQTLTYRDMRYAGGALTATIFVEPGRTAPAFLLLYPDPALVGKS
                     VERIQQGASMASYGFFYVSYGTAGKSPVTFAESGTLEVDSAYDVRRSVPAGG"
     gene            complement(1699..2820)
                     /gene="ald"
                     /locus_tag="VFX38_07595"
     CDS             complement(1699..2820)
                     /gene="ald"
                     /locus_tag="VFX38_07595"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX5314747.1"
                     /translation="MLIGVPKEIKIREYRVGMTPAGVRDLTGAGHRVLVETNAGAAID
                     FDDDAYRAAGAQIVADPLALFEQAEMIVKVKEPQPVECERLRPGQILFTYLHLAPDPR
                     QTKMLVDSDAIAIAYETVTDRHGRLPLLAPMSEVAGRLAIQAGAHSLEKAQGGRGVLL
                     GGVPGVPPARVVVIGGGVVGINSARLAMGAGARVVVLDKSLERLAELDNLYGPRLETL
                     YATTDSIEASVREADLVVGAVLVPGAAAPKLVTRELLTKMPRRAVVVDVAIDQGGCFE
                     TSRPTTHDEPTYVEEDIVHYCVANMPGAVPRTSTLALTNATLPFVHALASKGYRRALA
                     DDPHLREGLNVHRGKITCRAVADSLGLNCVPALDVLQAA"
     gene            2951..3268
                     /locus_tag="VFX38_07600"
     CDS             2951..3268
                     /locus_tag="VFX38_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HEX5314748.1"
                     /translation="MIVTTTENVPGRHTDRALGQVYGVVVRSRGVAGNVMAGLRSIMG
                     GEIREYTELLEEARRHAIDRMVENATAMGANAVVMMRFDSSEIGQTMSEVVAYGTAVV
                     LAE"
     gene            3265..3573
                     /locus_tag="VFX38_07605"
     CDS             3265..3573
                     /locus_tag="VFX38_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314749.1"
                     /translation="MTLRGSLLVLAVAAAIAGIVLAVLRIAPGLPLAIGAGVFAILVA
                     GESWRYRHRRPAADASGFQTTAERYRDPGTGEWMAVEYDPRSGARRYVKADGPPLEGS
                     "
BASE COUNT          632 a         1152 c         1289 g          672 t
ORIGIN      
        1 agccggggta gctcagtctg tcccccattt gtcgccattt gtcggttgac agggctgcaa
       61 ttctgcgcga agattcgaaa cgctcatttc gtcagggaga ataggatgaa ccgtattgct
      121 ggattcacat cggtttgcgc agcgctgctg cttctgggcg gttgcgcgac gtatcagagt
      181 gtcccgttca acggcgcttc gggccagcgg ccgcagcgaa ttggcctgat gaccgtctac
      241 tcgccgccgg gattcaccgt gtccattcga acgccggtcg gggccagctt tggtctgatc
      301 gggggagcca tcgcggcggg cgagatcaac gaaaaatcgg cggaattcac gaaagccgtc
      361 gcggcgcagg ggttctcggt gcaacaggcc ttgaccgagc aactgaaggc ggacttggaa
      421 gcggagggct acgcggtcga tctcgtgccc gccacgagaa gcggcgactc ctttctgcgc
      481 agctatccgc aggggagcgg cggcgaggcc tggctcgacg tagtggtcaa ggaccattcc
      541 gtcgggtacc gcgctgcggg aaacaagacg agctattatc cgtatgtcct tgtttcagca
      601 caattggtcg gccccggcgg cggggtgctc tattcgcagc agctcgtcta caatccgatc
      661 catccgccga aaaatgcacg cacgatcgcg ccgagcggcg agtatgcgta cgcgaacttc
      721 gatcagttga tggccgatcc cgcccgggcg accgccggac tgaaacaggc cgtcgcgcaa
      781 atcgcggccg cgatcgccgc ggatctgaaa tagcgctcga ggccggaatg cgacgactcg
      841 cggcggcggc gatggtggcg atctgcggcc tgctggccgc ctgcacgcag ccggtgccaa
      901 cggctgcgcc cttgtctgcc gggtatctca tggcgggtcg cagttgcggc ggcggcgcga
      961 cgctgccggc aacggcgctc acgatcggga cgcggcaggt gattcatctg gcggaatcga
     1021 gtccctgcat taccgccgga tccggcgtga acgagcccgc ggtgctcctg agtctgccgg
     1081 tttcgtccga cacgtattac gtgacggtcc gcgtctggcg cggagcgaaa atcgtggtgc
     1141 cgcgcatcga actgctcgac ggaaaccggc ggcccgtaca gacgttgacg tatcgggaca
     1201 tgaggtacgc cgggggtgcg ttgacggcga cgattttcgt cgaacccgga cgaaccgctc
     1261 cggccttcct gttgctctat ccggaccccg cgctcgtcgg aaaaagcgtc gagcgaatcc
     1321 agcaaggggc ttccatggca agctacggtt ttttctatgt gtcgtacgga accgccggga
     1381 aatcgccggt gacctttgcg gaatcaggca cgctcgaagt cgacagcgcc tacgatgtga
     1441 ggcggagcgt ccccgcgggc ggttagcggg ccggagtcgg agactccgtt tgtcgttgcc
     1501 gcggccgttt cgaatgcgac cgcgactttc cagtgcaacg ccgatcgagt ctgacgtggt
     1561 cattccggaa cggcggtgcg ttgtccggca tccgttacca ggaaacgaat cccggcctgc
     1621 gcgcgggata cattctctcg actgggtccc ggccttcgcc ggaacgacga gacggagcaa
     1681 tccctgatcc gcgttgccct aggcggcttg cagcacgtcc agcgcgggaa cgcaattcag
     1741 acccagcgag tccgctacgg cccggcaggt gatcttgccg cgatgcacgt tgagcccttc
     1801 gcggagatgc ggatcgtcgg caagcgcgcg gcggtatccc ttgcttgcga gcgcatgcac
     1861 gaaaggcagg gtcgcgttgg tgagcgcaag cgtcgaggtg cggggcacgg cgccgggcat
     1921 gttggcgacg caatagtgca cgatgtcttc ctcgacataa gtaggctcgt cgtgcgtggt
     1981 gggacggctc gtttcgaagc agccgccctg gtcgatggcg acgtccacga cgaccgctcg
     2041 ccgcggcatc ttcgtcagaa gttcgcgggt cacgagtttc ggcgccgcgg cgccgggaac
     2101 cagcaccgcc ccgacgacga gatccgcttc gcgcacgctg gcctcgatgc tgtccgtcgt
     2161 tgcgtagagc gtttcgaggc gcggtccgta cagattgtcg agttcggcga ggcgttcgag
     2221 tgatttgtcg agcacgacga cgcgggcgcc cgcacccatg gcgaggcgcg ccgagttgat
     2281 cccgacgacg ccgccgccga tgacgacgac ccgtgccgga ggaacgccgg gaacgccgcc
     2341 gagaagaacg ccgcggccgc cctgggcttt ttcgagcgag tgggcgcccg cctgaatcgc
     2401 gaggcggccg gcgacttcgc tcatcggggc gagcagcggc aggcggccgt ggcgatcggt
     2461 cacggtctcg taggcgatgg cgatggcgtc cgaatcgacc agcatcttgg tttgccgcgg
     2521 atcgggcgcg agatgcagat aggtgaagag tatctgcccc ggacgcaggc gctcgcattc
     2581 caccggttgc ggctccttga ccttgacgat catctccgcc tgttcgaata gcgcgagggg
     2641 atcggcgaca atttgggcac cggcggcgcg ataggcgtcg tcgtcgaagt cgatggcggc
     2701 accggcgttc gtttccacca gcacccgatg cccggcgccg gtcaggtcgc gaacgcccgc
     2761 cggagtcatg ccgacgcggt attcgcgaat cttgatttct ttgggtacgc cgatgagcat
     2821 ggctgggaaa cctccggatg cggtggggcg ggcggtcgag gtggcacgaa accggataat
     2881 tttaccaaag ttcacgccgc cgcagcgtca ctcggtatac actgaaaact ctttccccga
     2941 gagttgaccg atgattgtca cgacgacgga aaacgtgccc ggcagacaca ccgaccgggc
     3001 cctgggccag gtctatggcg tcgtggtgcg cagtcgcggc gtggccggta acgtgatggc
     3061 cggtctgcgt tccatcatgg gcggcgagat tcgcgaatac accgagcttc tggaagaagc
     3121 acgccggcac gcgatcgacc gcatggtcga gaatgcgacc gcgatgggag cgaacgcggt
     3181 ggtgatgatg cgcttcgatt cttcggaaat cggccagacc atgagcgaag tcgtcgccta
     3241 cggcaccgcg gtggtcctcg cggaatgaca cttcgcggct ctcttctcgt tctcgccgtc
     3301 gcggccgcga tcgcgggaat cgtgctcgcc gttctgcgca tcgccccggg cctgccgctc
     3361 gcgatcggcg ccggagtgtt tgcgattctc gttgcgggcg aaagctggcg ctaccggcat
     3421 cggcgccctg cggcggatgc gagcggattt caaacgacgg ccgagcgcta tcgcgatccc
     3481 gggaccggcg agtggatggc ggtcgaatac gatccgcgca gcggcgcgcg acgttacgtc
     3541 aaggccgacg gcccgccgct cgaaggctcc tgagattttc ggaaatccgc ggccgggcga
     3601 cgcgcctgtg cagcaccgat cagtccgcat ccactgcgac ttcgacgcgc ccgtcgcgga
     3661 tgcgtacggc ataggttggg acgtcttcgt aagcgggagg tttgagcact gcgcccgtgc
     3721 ggatgtcgaa gcgcgcgccg tgtcg
//
LOCUS       DASUJG010000353         3133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1080215, whole genome shotgun sequence.
ACCESSION   DASUJG010000353 DASUJG010000000
VERSION     DASUJG010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080215"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VFX38_07610"
     CDS             <1..408
                     /locus_tag="VFX38_07610"
                     /inference="COORDINATES: protein motif:HMM:NF028497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HEX5314750.1"
                     /translation="SAQARAKIGAKALALVTRDENAASRDAVGFCPAEHGGKLAPQGL
                     PPGFRVLRTVCEHQSGLDGPVTHLLVGDGLAMVSVFIAPYRSNGTALIGVTALGAVHA
                     VGRVAGGYAITAMGDAPSITISRIASAVEVTKH"
     gene            481..2277
                     /gene="lepA"
                     /locus_tag="VFX38_07615"
     CDS             481..2277
                     /gene="lepA"
                     /locus_tag="VFX38_07615"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX5314751.1"
                     /translation="MRRIRNFAIIAHIDHGKSTLADRLIERCGGLAMREMAAQVLDSL
                     AIERERGITIKAQSVRLGYDARDGERYQLNLIDTPGHVDFAYEVSRSLAACEGALLVV
                     DSSQGVEAQSVANCYSAVEQGLEVIPVLNKIDLAAAEPERVAAEIEDLIGIEARGALR
                     VSAKTGAGIEELIEALIARVPPPAGDPEAPLQALILDSWFDSYEGVVSLVRIKSGALR
                     KGNRIQVMSTGGLWYVTRIGCFTPRMQDTGLLDCGEVGFVIAGIKEIDGAPVGDTITL
                     GDRPAARPLPGFAPLKPRVFAGLFPSDASAFENLREALAKLRLNDAALAYEPETSGAL
                     GFGFRCGFLGRLHMEIVQERLEREYGLDLISTAPSVVYRVVTTTGSVLEVENPAALPP
                     RGEIERIEEPVISATILTPHDYVGPVIGLCEEKRGSQKSLVYHTGQVALTYDLPLAEV
                     VLDFFDRLKSLSRGFASFDYGPARFVEAPLVRLDVLINQERVDALSLIVHRDHAFSRG
                     RALTERLRDLIPRQMFEVAIQAAIGGQVIARSSVKALRKNVTAKCYGGDITRKRKLLE
                     KQKEGKKRMKRVGRVDVPQSAFLAILQSERQS"
     gene            2317..>3133
                     /gene="lepB"
                     /locus_tag="VFX38_07620"
     CDS             2317..>3133
                     /gene="lepB"
                     /locus_tag="VFX38_07620"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX5314752.1"
                     /translation="MNYAVVILILVAITGFLWLLDILLRRVFLRGREFPGAWRWLADT
                     GRGFFPLLLAVFLLRSFVVEPFHIPSGSLMPTVLVGDFVATEKYAYGLYIPILHKKFI
                     STGSPHRGDIFVFHFPVDSALKHCQGDPLCAASGEANLFRTYAGVDFIKRVIGLPGDT
                     IGYTCDNQLVINGRKVRREYLGIYHGEGSEAQFTGEQVWREYLPQPDGSIVRHRILLM
                     PDRPIGPPYCGRTWTVAPNSYFAMGDNRDDSDDSRAWGDVPEANLVGRARLVFF"
BASE COUNT          525 a         1038 c         1004 g          566 t
ORIGIN      
        1 agcgcgcagg cgcgcgcgaa gatcggagcc aaggcgctcg cgctggtgac ccgcgacgaa
       61 aacgccgcgt cccgcgatgc ggtcggcttc tgcccggcgg aacacggcgg caagctcgcg
      121 ccgcaaggcc ttccgcccgg atttcgcgtg ctgcgaacgg tgtgcgaaca tcagagcggg
      181 ctcgacggcc cggtcaccca tcttctcgtg ggcgacggcc tcgccatggt ttccgtgttc
      241 atcgctccct atcgcagcaa cggcaccgcg ctgatcggag tcacggcgct gggcgcggtg
      301 cacgcggtcg gaagggtcgc gggcggctac gccatcaccg ccatgggcga cgctccctcg
      361 atcacgatct cgcgaatcgc gagcgcggtc gaggttacca aacattgaag cgggcaggcg
      421 gttcgcgccc gcacgatgcc ccgccggtat aatcgcgact ttgccggcag ccccctgttg
      481 atgcggcgaa tccgcaattt cgcgatcatc gcgcacatag atcatgggaa atccactctc
      541 gccgaccggc tgatcgaacg ctgcggcggg ctcgccatgc gcgagatggc ggcccaggtg
      601 ctcgattcgc tcgcgatcga gcgcgaacgc gggatcacca tcaaggcgca gagcgtgcgg
      661 ctcggctacg acgcgcgcga cggcgaacgc tatcaattga acctcatcga cacccccggg
      721 catgtggact tcgcctacga ggtgtcgcgt tcgctcgcgg cctgcgaagg cgcgcttctg
      781 gtcgtcgaca gttcccaggg cgtggaggcg caaagcgtcg ccaactgtta cagcgcggtg
      841 gagcaggggc tcgaagtcat cccggtgctg aacaagatcg atcttgccgc cgccgaaccc
      901 gagcgcgtcg ccgccgaaat cgaagacctg atcggcatcg aggcgcgcgg agcgctgcgc
      961 gtgagcgcga agaccggagc gggcatcgag gaactcatcg aggcgctgat cgcgcgcgtt
     1021 ccgccgccgg ccggcgatcc cgaggctccg ctgcaggcgc tgatcctcga ttcctggttt
     1081 gactcttacg aaggcgtggt ctcgctcgtg cggatcaagt cgggtgcgct gcgcaagggg
     1141 aaccgcattc aggtgatgtc gacgggcggc ctctggtatg tgacccgcat cggctgtttc
     1201 accccgcgga tgcaggatac ggggctcctc gactgcggcg aggtgggctt cgtcatcgcg
     1261 ggaatcaagg agatcgacgg ggctcccgtc ggagacacca tcacgctcgg cgatcgtccg
     1321 gccgcgcggc cgctgccggg atttgcgccg ctcaagccgc gggtgttcgc gggattgttc
     1381 ccctccgatg catcggcctt cgagaatctg cgcgaggcac tcgcgaagct gcggctgaac
     1441 gacgccgcgc tcgcctacga gccggaaacc tcgggcgcgc tcggcttcgg attccgctgc
     1501 ggttttctcg ggcgcctgca catggaaatc gtgcaggagc gtctcgagcg cgaatacgga
     1561 ctcgatctca tctccaccgc gccctcggtc gtgtatcgcg tggtgacgac gacggggagc
     1621 gtgctcgaag tcgaaaatcc ggcggccctg ccgccgcggg gtgaaatcga acgcatcgag
     1681 gagccggtca tttcggcgac gattctcacg ccgcacgatt acgtcggacc ggtcatcggt
     1741 ttgtgcgagg aaaagcgcgg cagccagaag tccctcgtct accacaccgg ccaggttgcg
     1801 ctcacctacg atctgccgct cgccgaggtg gtgctcgatt tcttcgaccg gctgaagtcg
     1861 ctcagccgcg gcttcgcctc cttcgattat ggcccggcgc gttttgtcga agctccgctg
     1921 gtgcgcctcg atgtgctgat caaccaagaa cgcgtcgatg cgctctcgct catcgtgcat
     1981 cgcgatcacg ccttctcccg cggccgcgcg ctcaccgagc ggctgcgcga tctcattccg
     2041 cgccagatgt tcgaagtggc gatccaggcg gcgatcggcg gccaggtgat cgcccgctcg
     2101 agtgtcaagg cgctgcggaa aaacgtgacc gccaagtgct acggcggcga catcacgcgc
     2161 aagcgcaagc tgctcgaaaa gcagaaggaa gggaagaagc gcatgaagcg cgtcggccgc
     2221 gtggacgttc cgcaatccgc gtttctcgcg atattgcaga gcgaacggca atcatgaggc
     2281 agcgcctcat cagcaatccg caagcgagac attgacatga actacgccgt cgtcattttg
     2341 attctggtcg cgatcacggg cttcctgtgg ctgctcgaca tcctgctgcg ccgggtcttc
     2401 ctgcgcggcc gggaatttcc cggcgcctgg cgctggcttg ccgacaccgg ccgcgggttc
     2461 tttccgctcc ttctcgccgt ctttctgctg cgcagtttcg tcgtcgagcc ctttcacatt
     2521 ccgagcggct cgctcatgcc gacggtgctc gtcggcgatt tcgtcgcaac cgaaaaatac
     2581 gcctacggcc tgtacattcc gatcctccac aagaaattca tttcgaccgg cagtccgcat
     2641 cgcggcgaca ttttcgtctt tcattttccc gtcgactcgg cgctgaaaca ttgtcaggga
     2701 gatcccctct gcgccgcgag tggcgaggca aatctcttcc gaacctatgc cggggtggac
     2761 ttcatcaagc gggtgatcgg tcttcccggc gacaccatcg gctacacctg cgacaatcag
     2821 ctcgtcatca acggcaggaa ggtgcgccgc gagtatctcg gcatctacca cggcgaaggc
     2881 tcggaagccc agttcacggg cgagcaggta tggcgcgagt atctcccgca gcccgacggg
     2941 agcatcgttc gtcaccgcat tctgctcatg ccggatcgcc cgatcgggcc gccctactgc
     3001 ggtcggacct ggaccgtagc gccgaactcc tatttcgcga tgggcgacaa ccgcgacgac
     3061 tccgacgaca gccgcgcctg gggcgacgtg cccgaggcca atctcgtggg ccgcgcccgg
     3121 ctcgtgttct tca
//
LOCUS       DASUJG010000354         2586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1201802, whole genome shotgun sequence.
ACCESSION   DASUJG010000354 DASUJG010000000
VERSION     DASUJG010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201802"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VFX38_07625"
     CDS             complement(<1..639)
                     /locus_tag="VFX38_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314753.1"
                     /translation="MKGGNVLFSVPRIYPRRIALAVMFLMTARTFYFFPGMSIPSEVW
                     NLLSAGFLAFVYLGWKIKVKGRFETFEFYVLILMVLMPLWSGFMAMQVFGQPMIFGVL
                     SQRTMVLGTGALALLLFYKAGWIDSNACRQAFLIPAWATLILYSAYQVYLQTGFSLNA
                     PKARFVYAFVEIGFYYYAITGFNRKSKAHFLLALPFFVYIISTGQRALLLTTI"
     gene            complement(676..1605)
                     /locus_tag="VFX38_07630"
     CDS             complement(676..1605)
                     /locus_tag="VFX38_07630"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX5314754.1"
                     /translation="MPETEFRVICGIARSGTTISREIARRAVPGVLLEEPLHRSRGVA
                     GVPVAYPYVSAKGGEYAELIDEITRFARPWGKHPASASWGKVKRAIFRVAGDEWWLRW
                     RLLRLRAGLGFAPRRVLWKDPFASLATPYLLARHGARVVCMVRHPAAVYVSMCKWGWG
                     FNIAYLRDQPELMEAQGCDILPRDWERARASTAASVAILWKVMVALNAPLADADSRLM
                     LVRHEDLCDDPMTVSRALVKQLGGEMTASAQRFMNRSFHGASAEGNSLRPHDFVRDIR
                     ALGDSWRRKVSPEDESAMRDIIGDDFHRIYAHW"
     gene            complement(1665..>2586)
                     /locus_tag="VFX38_07635"
     CDS             complement(1665..>2586)
                     /locus_tag="VFX38_07635"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX5314755.1"
                     /translation="SGSDGRGGCGKVSEATAKPLPPVIVIGMHRSGTTMIARMLSDLG
                     VFMGADLERNHESLFFIACNDWLLGNSGGRWDNPGAMKWLQASDEMLQLSEEYLAYRL
                     RHAPARHYTGLARYVAGSRALPGLTCPWGWKDPRNTFTLPFWLRLFPAARIVHIYRNG
                     VAVAGSLCARAKEEFVRGRSKHRHRLERGLYRWRAKRSGFGASPRALDLRGAFSLWEE
                     YLAAAFDVTERGSGSVFAVKYEDFLSAPEAGVARLASFCGLAPPASVLARIAVVADGG
                     KREGFLADPELLTMQNELSDNPWMKRLGYV"
BASE COUNT          568 a          835 c          765 g          418 t
ORIGIN      
        1 gatcgtggtg agcagcaggg cacgctgacc ggtcgagata atgtaaacaa aaaacgggag
       61 cgcgagcaaa aaatgcgcct tcgattttcg gttgaatccg gtaatggcgt aatagtaaaa
      121 cccgatttcg acgaacgcat agacgaaacg ggccttcggt gcgttcaggg aaaaccccgt
      181 ctgcaaatag acctgatagg cgctgtagag aatcagggtg gcccaggcag gtatgaggaa
      241 cgcctggcgg caagcgttgc tgtcgatcca gccggccttg tagaaaagca gcaacgcgag
      301 cgcaccggtg ccgaggacca tcgtgcgctg tgacaatacc ccgaagatca tcggctggcc
      361 gaagacttgc atcgccatga agcccgacca gagcggcatg agaaccatca ggatgaggac
      421 ataaaactcg aaggtctcga aacgcccctt gaccttgatc ttccagccca gatacacgaa
      481 agccagaaaa ccggccgaaa gcaggttcca gacttcgctc ggaatcgaca ttcccgggaa
      541 aaagtaaaaa gttcgggccg tcatgagaaa catcacggca agtgcgatgc ggcgaggata
      601 gatgcggggt acgctgaaca gtacgttgcc gcccttcacc ctcaatccgg aagacaatgt
      661 tcgattcacg ctccttcacc aatgcgcata gattcgatga aagtcatcgc cgatgatatc
      721 cctcatcgcg gattcatcct ccggagaaac cttgcgacgc cacgagtcgc cgagggcacg
      781 gatgtcgcgg acgaaatcgt gcggcctcag cgagtttccc tcggcgcttg ctccatgaaa
      841 actgcgattc atgaagcgtt gtgccgacgc ggtcatttct ccgcccaatt gcttcaccag
      901 cgcccgggat acggtcatcg ggtcgtcgca caaatcctcg tggcgcacca gcatcaggcg
      961 cgagtcggcg tcggccagcg gcgcgttgag agcaaccatg accttccaga gtatcgcgac
     1021 ggaagccgca gtactcgcgc gagcccgctc ccaatcgcgc ggcagtatat cgcagccctg
     1081 ggcttccatg agttccggct ggtcgcgaag ataggcgata ttgaaacccc agccccattt
     1141 gcacatgctc acgtatacgg cggccgggtg ccggaccatg cagaccaccc gcgccccgtg
     1201 ccgcgcgaga agataggggg ttgccaagct cgcgaacgga tccttccaca agaccctccg
     1261 cggagcgaat ccaaggcccg ctcgcagccg cagaagccgc cagcgcagcc accactcgtc
     1321 gcccgcgaca cgaaatatcg cccgtttgac ctttccccag gacgccgagg cggggtgctt
     1381 tccccaaggc cgcgcgaacc gcgttatctc gtcgataagc tcggcatact cccccccctt
     1441 ggcggagacg taggggtagg cgacaggcac gccggcgacg ccgcggctgc gatgcagggg
     1501 ttcctccagc agaaccccgg gaaccgcccg gcgggcgatc tcgcggctga tcgttgtacc
     1561 gctgcgggcg atgccgcata tcacccgaaa ctccgtctca ggcatcgccg caaaccgcag
     1621 ggcgaacacc gagcgagccg cgtgtaccct gcctcgcagc gccttcaaac gtatccaagg
     1681 cgcttcatcc atggattgtc cgacagctca ttctgcatcg tgagcaattc cgggtcggcg
     1741 agaaacccct cgcgtttgcc gccatccgcc acgacagcaa tgcgagcgag gacggacgcg
     1801 gggggggcca ggccgcagaa ggaagcgaga cgcgcaacac ccgcctccgg cgccgacagg
     1861 aaatcttcgt atttcaccgc gaacacggaa ccgctgccgc gttccgtcac gtcgaacgcc
     1921 gcggccagat actcttccca aagcgagaag gctccgcgca ggtcgagagc gcgaggcgag
     1981 gccccgaaac ccgatctttt ggcgcgccag cgatacagac cgcgctcgag acgatgccgg
     2041 tgtttgctcc gcccgcggac aaactcctct ttcgcccgtg cgcacaaact gccggcaacg
     2101 gcgaccccgt tgcggtagat gtgtacgatc cgggccgcgg gaaagagccg cagccaaaac
     2161 ggcaatgtga aggtgtttct cgggtccttc cagccccaag ggcaggtgag acccggaagc
     2221 gcgcgcgaac cggcaacgta gcgggcaagg ccggtgtagt gccgggccgg agcgtggcga
     2281 aggcgatacg cgagatactc ttcgctgagc tgcaacatct cgtcgctcgc ctgcaaccac
     2341 ttcatcgccc ccggattgtc ccagcgtccg ccgctgttgc ccagcagcca atcgttgcag
     2401 gcgatgaaaa acaaggactc gtgattgcgt tccaagtctg ctcccatgaa cacgccgagg
     2461 tcggagagca tccgcgcaat catggtcgtt cccgaacgat gcatcccgat cacgataacg
     2521 ggcgggagcg gcttggccgt cgcctcgctc accttgccgc atcctccgcg gccatcgctt
     2581 ccagac
//
LOCUS       DASUJG010000355         3872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_346599, whole genome shotgun sequence.
ACCESSION   DASUJG010000355 DASUJG010000000
VERSION     DASUJG010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346599"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2610)
                     /gene="leuS"
                     /locus_tag="VFX38_07640"
     CDS             complement(<1..2610)
                     /gene="leuS"
                     /locus_tag="VFX38_07640"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007972619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX5314756.1"
                     /translation="MKRDRATREPVEHYEPAAVEAQVQALWSERNAFAAREDSGRDKF
                     YCLSMLPYPSGQLHVGHVRNYTIGDVIARYRRMRGANVLQPIGWDAFGLPAENAAIQH
                     RRAPGEWTRGNIAEMRAQLRRMGFAFDWSREFATCDPGYYRWEQWFFLRLFERGLVYR
                     ANSIVNWDPVDRTVLANEQVVDGRGWRSGAVVEQRQVPQWFLKITAYADELLAELDRL
                     PGWPEEVKIMQRNWIGRSEGLDIRFAIAGETEPLQVFTTRPDTLLGVSFLAIAPEHPL
                     ALRAAGSDPQLAGFLAQCRRGSVSEAAVETREKRGMPTGFVALHPLTGERLPVWVANF
                     VLIAYGSGAVMGVPGHDRRDFDFAHACGLPIRQVIANEAEDYDAAEWRDWYEASDARL
                     RVVNSGFGLDGLAHREAFARVAELLAERGAAEPRVQYRLHDWGVSRQRYWGCPIPMIH
                     CPDCGAVPVPEEDLPVVLPENVRFDGVASPLTTDPEWRLVRCPRCGGAAERETDTFDT
                     FMESSWYFVRYCSPDAAAMTDGRARYWLPVDQYVGGVEHAVLHLLYARFFYKCMRDAG
                     LVPGDEPFTRLLTQGMVVADTWYRDEGNTRHWFNPAEVVVERDAKGALLAARLAADGE
                     ALRFGGVEKMSKSKNNGVAPEPLLERYGADALRLFSMFAAPPDQALEWSDAGIEGAAR
                     FLRRLWRLVRDPPRHEAGETGGSADDAIALRRKLHQTIVKVSDDLERRYAFNAAIAAN
                     MELANDLARFRPRSPGDDALVDETLRAMLRMLAPVVPHIAERLWQSLGEDGLIMDAAW
                     PEADPEALASDTIRLVVQVNGRLRGHIAVPAGATPAQIERCALAEENVRRHVGDRAVR
                     RVIVVPERLVNVVV"
     gene            complement(2607..3275)
                     /locus_tag="VFX38_07645"
     CDS             complement(2607..3275)
                     /locus_tag="VFX38_07645"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006100652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5314757.1"
                     /translation="MSAHTLPLTETLYRYLLDHSLREPPVLARLREETQLMAQASMQI
                     SPEQGQFLAMLVRLIGARRCLEVGSFTGYSSTAVALALPPDGRLLCLDVSEVWTARAR
                     RAWQQAGVENKVELRLAPARESLEALIAGGAGETFDFAFIDADKTNYLHYYEAALALL
                     RPGGLIAVDNVLWGGSVADPADQSEDTIAIRAFNDALHADRRVDLSLLSIGDGLTLAR
                     KREA"
     gene            3375..>3872
                     /locus_tag="VFX38_07650"
     CDS             3375..>3872
                     /locus_tag="VFX38_07650"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEX5314758.1"
                     /translation="MPSSSATTQSRAPSRLWKTSASAIAFLLLAFPAASVLAASHPLP
                     LWRVSDGRGHVLYLAGAMHALTPVDYPLPAAMTNAFANSDRLVEELDLAAISPVAAAQ
                     TLAAKGRLPEGQSLAGAMGEDWKRAQSLAKAGGLDLAAYDKLRPWLAAVEIAEQAVSD
                     AGYRPA"
BASE COUNT          635 a         1335 c         1294 g          608 t
ORIGIN      
        1 gacgacgaca ttgaccagcc tttccggcac gacgatgacc cggcgcaccg cgcgatcgcc
       61 cacgtgtcgc cgcacgttct cttcggccag ggcacagcgt tcgatttgcg ccggcgtcgc
      121 cccggcggga acggcgatat gcccgcgaag ccggccgttc acctggacca cgagccggat
      181 cgtgtcgctt gcgagcgcct ccggatccgc ctcgggccag gccgcgtcca tgatcaggcc
      241 gtcttctcca agcgactgcc acagccgctc ggcaatgtgc ggcaccaccg gcgcgagcat
      301 ccgcagcatc gcgcgcagtg tctcatccac cagcgcgtca tccccgggcg agcgcgggcg
      361 gaaacgcgcg aggtcgttcg cgagttccat gttcgccgcg attgcggcgt tgaaggcata
      421 gcgccgctcg agatcgtcgc tcaccttgac gatggtctgg tgtagcttgc ggcgcagcgc
      481 gatcgcatcg tccgcgctgc ctccggtttc gcccgcctcg tgacgcggcg gatcgcgcac
      541 cagccgccac aggcgacgaa gaaaccgcgc cgcaccctcg atgccggcat cggaccattc
      601 gagcgcctga tcgggcggcg ccgcgaacat cgaaaagagc cgcagcgcgt cggcgccgta
      661 gcgttccagg agcggttccg gggccacgcc gttgttcttc gatttggaca ttttctcgac
      721 gccgccgaag cgcagcgctt cgccgtcggc cgcgagtcgg gccgctagca gcgcgccctt
      781 cgcgtcccgc tcgaccacga cctcggccgg attgaaccaa tgccgcgtgt ttccttcgtc
      841 gcggtaccag gtgtcggcga cgaccatgcc ctgggtgagc aggcgcgtga acggctcgtc
      901 cccgggaacg agtccggcat cgcgcatgca cttgtagaaa aaacgcgcgt agagaagatg
      961 cagcaccgcg tgctcgacgc cgccgacgta ctggtccacg ggcagccagt agcgcgcgcg
     1021 cccgtcggtc atggccgcgg cgtcgggcga gcagtagcgc acgaagtacc acgacgattc
     1081 catgaaggta tcgaaggtgt cggtctcacg ctccgccgcg ccgccgcaac gcgggcagcg
     1141 caccagccgc cattcgggat cggtcgtgag cggcgaggcg acgccgtcga agcggacgtt
     1201 ctcggggagc acgaccggca gatcctcctc gggcaccggt accgcgccgc aatcggggca
     1261 gtgaatcatc ggaatgggac aaccccagta acgctggcgc gagactcccc aatcgtgaag
     1321 acgatattgc actcggggtt cggcggcgcc gcgttcggcg agcagttccg caacgcgcgc
     1381 gaaggcctcg cggtgagcga ggccgtcgag accgaaaccc gaattcacca cccgcagccg
     1441 cgcgtcgctc gcctcatacc agtcgcgcca ttcggcggcg tcgtaatcct ccgcttcgtt
     1501 tgcaatcacc tgccgaatcg ggagcccgca ggcatgcgca aaatcgaagt cgcgccgatc
     1561 atggcccgga actcccatga cggcaccgct gccgtaggcg atcagcacga aattcgccac
     1621 ccacacgggc aggcgttcgc cggtcagcgg atgcagcgcc acgaagcccg tcggcatgcc
     1681 gcgcttttcg cgcgtttcca ccgctgcttc gctcaccgag ccgcgccggc attgggcgag
     1741 aaagccggcg agttgcggat cgctcccggc ggcacgcaga gcgagcgggt gctcgggcgc
     1801 aatggcgaga aagctcacgc ccagcagcgt atcggggcgg gtcgtaaaaa cctgcagcgg
     1861 ttcggtttcg ccggcgatcg cgaagcgaat gtcgagcccc tcggagcgcc cgatccagtt
     1921 gcgctgcatg atcttcacct cctccggcca gccggggagg cgatccagtt ccgcaagcag
     1981 ttcgtcggcg taggccgtga ttttcagaaa ccactgcggg acctgtcgtt gctcgaccac
     2041 ggctcccgag cgccagccgc ggccatccac gacctgctcg ttggcgagca cggtgcgatc
     2101 gaccggatcc cagttcacga tggagttggc gcgatacacg agtccgcgct cgaacagacg
     2161 aaggaaaaac cattgctccc agcggtagta gccggggtcg caggtcgcga actcgcgcga
     2221 ccagtcgaag gcgaatccca tgcgccggag ttgcgcacgc atttcggcga tgttgccccg
     2281 cgtccattcg cccggcgccc tgcgatgctg gatcgccgca ttctccgcgg gcaggccgaa
     2341 ggcgtcccag ccgatcggct gaagtacgtt tgcgccgcgc atgcgccggt agcgtgcaat
     2401 cacgtcgccg atcgtgtagt tgcgcacgtg cccgacatgc aactggccgc tcgggtacgg
     2461 cagcatcgac agacaataga acttgtcgcg cccggaatcc tcgcgcgcgg caaacgcatt
     2521 gcgttcgctc cagagcgcct gcacctgcgc ttcgacggcg gccggctcgt agtgctcgac
     2581 cggctcgcgc gtcgcgcgat cccgcttcat gcctcgcgct tgcgggcgag cgtgagtccg
     2641 tctccgatgg aaaggagcga gagatcgacg cgtcggtccg cgtggagcgc gtcgttgaac
     2701 gcgcgaatgg cgatggtgtc ttcgctctgg tcggccgggt ctgcgacact tccgccccag
     2761 agcacgttgt cgaccgcgat caggccaccg gggcgaagca gcgcaagcgc cgcctcgtag
     2821 taatggagat agttcgtctt gtcggcgtcg atgaaggcga aatcgaaggt ctctccggcg
     2881 ccgccggcga tcagcgcctc gaggctttcg cgagcgggcg caagacgcag ctccaccttg
     2941 ttctcgacgc ccgcctgctg ccaggcgcgg cgggcgcgcg ccgtccacac ttcgctcacg
     3001 tcgagacaga gcagacggcc gtccggcggc agcgcgagtg cgaccgcggt gctggaatat
     3061 cccgtgaagc tgccgacctc gagacagcgg cgcgcgccga tgagtcgcac cagcatcgcc
     3121 agaaactggc cctgttcggg agagatctgc atgctcgcct gtgccatgag ctgcgtctct
     3181 tcgcgcagtc tcgcgagcac cggcggttca cgcagggaat gatccagcag gtagcgatac
     3241 agcgtctcgg tgagcggcag ggtgtgggcg gacatgactc gggcggcgat cgggaagagg
     3301 gccattctag cgaaccgcgg cggacccggc gacggctcgc gatcgggtgg gacgcacggc
     3361 gtacaatggc cccgatgcct tcgagcagcg ccacgacgca atcccgggcg ccatcgcggc
     3421 tgtggaagac ctctgcctcc gcgatcgcgt ttctcctgct cgcattccct gcggcgtcgg
     3481 tgcttgcggc ctcccatccg ctgccgctgt ggcgggtcag cgacggccgc ggacacgtcc
     3541 tctatctcgc cggcgccatg catgcgctca cgcccgtcga ctatccgctg ccggcggcga
     3601 tgacgaatgc gttcgcgaat tccgaccgcc tcgtggaaga actcgatctc gccgccattt
     3661 cgcccgtcgc agcggcgcag acgctcgcgg ccaaaggccg cttgccggag gggcagagcc
     3721 ttgccggggc gatgggcgag gactggaaga gggcgcagtc cctcgcgaag gcgggcggcc
     3781 tcgatctcgc tgcctacgac aagctgcgac cgtggctcgc ggccgtcgag atcgccgagc
     3841 aggcggtgag cgatgccggc tatcggccgg ct
//
LOCUS       DASUJG010000356         3272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1813215, whole genome shotgun sequence.
ACCESSION   DASUJG010000356 DASUJG010000000
VERSION     DASUJG010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1813215"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VFX38_07655"
     CDS             <1..352
                     /locus_tag="VFX38_07655"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323013.1"
                     /note="catalyzes the reduction of 2 glutathione to
                     glutathione disulfide; maintains high levels of reduced
                     glutathione in the cytosol; involved in redox regulation
                     and oxidative defense; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEX5314759.1"
                     /translation="VDYEYVPTVIFSHPPVGTVGLTETRARERHGDAVRVYTQRFTPL
                     TSALTEDQQEAAIKLVTIGQDERIVGLHVVGDGADELLQGFAVAIRMGATKRDFDETI
                     AIHPTLAEEVVTLR"
     gene            complement(431..865)
                     /locus_tag="VFX38_07660"
     CDS             complement(431..865)
                     /locus_tag="VFX38_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008030796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX5314760.1"
                     /translation="MSNEKSYRGHCFCGAVELVASGEPMAMGYCHCESCRHWAAGPVN
                     AFTLWKPEAVKITKGAENIGSYSKNPQSIRHWCKVCGGHVFGEHPTLGFTDVYAAVIP
                     DLEYRPAVHVNYQETVLHISDGLPKLKDFPAEMGGSGETLAE"
     gene            complement(954..2126)
                     /locus_tag="VFX38_07665"
     CDS             complement(954..2126)
                     /locus_tag="VFX38_07665"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5314761.1"
                     /translation="MSLTVWAREAVSTCRFSLSSVRFRLQPAVIAVIGFCGVVLVFIG
                     LLSIRDGLTAVLAHTGSDDVAIVSNDGPGMTDLQTVRAIGEAPGVAQGPAGPLALGDL
                     VTSIMAHKDSGTLAAVTLRGVGPGFEHIWNKVQIVQGRLFRSGLDEVVVGRAASQQFP
                     QFRLGNTFQWNHHEWKVVGIFSSGGDMHESEVWADVHDVQAAYNAGNNFSGVYVKLTS
                     AAAFDGFEKALKSNPQLNVSVDRESTYFANASKDLDGVLTVAGGVIALLMGIGAVFGA
                     LITMYAAVAARTREIATLRALGFSRSPVVLSVVVEALLLGAAGGIVGGLIGYLLFNGF
                     KATTIGNGGMLAFQFAVTPSLLATGVIYALLMGLVGGLFPAIRAARLPIATAIRDV"
     gene            complement(2128..>3272)
                     /locus_tag="VFX38_07670"
     CDS             complement(2128..>3272)
                     /locus_tag="VFX38_07670"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5314762.1"
                     /translation="YWPLLWSNLKRKKPRTIFTLFAVMSAFALFGLLAAMQHGMNGVL
                     EIASAQRIQTNAKLGALPIAYAPKIESVPGVTTVAWLSGFDGYYQDEKNDMQVVAVSP
                     AAFLKVYPEILLSPQAREAWFGDRTGAIVGTLLAKRYGWKVGDQVPIRSKDLTKDDGS
                     NTWDLTIDGIYDADLPDRYKSFVMIHYPFFNDSIATGKDLVFQYIERISDPRAAPEIS
                     RAIDDKFANSFPQTRTESEVAETQTFLREFGNIGFMTLVIGAAVFFSMLLVTINTMSQ
                     AVRERTSELAVLKALGFGRGTVSGLVLAEAVGVTLIGGLLGLAAAWLLARAMYGSLSA
                     VLPALGMPAEAVGLGVLLMILFGLIAGALPLRQVFRLRAADALRRA"
BASE COUNT          606 a         1073 c         1059 g          534 t
ORIGIN      
        1 cgtggattat gaatacgtcc ccaccgtgat cttcagccac ccgccggtcg gcacggtagg
       61 acttaccgag acgcgggcgc gcgaacgtca cggggatgcg gtgcgcgtct acacgcagcg
      121 cttcacgccg ctcacatcgg ccctcaccga ggatcagcag gaagccgcga tcaagctcgt
      181 cacgattgga caggacgagc gcatcgtcgg cctgcacgtg gtcggtgatg gcgccgacga
      241 actgctccag ggcttcgccg tcgcgattcg catgggcgcg accaaacgcg acttcgacga
      301 aaccatcgcc attcacccga cgctcgccga ggaagtcgtc accctgcgct gatttcgcct
      361 ccgcccggtc cgcagcatcg cgtggccgcg tcgggaatcg gcgattcgcc aaacggcagg
      421 aacggaaaat ttattcggcc agcgtttcgc ccgatccgcc catctccgcc ggaaagtcct
      481 tgagcttggg aaggccgtcg gaaatgtgca gcaccgtttc ctggtagttg acgtgcacgg
      541 cggggcgata ctccaggtcc ggaatcaccg ccgcgtacac gtcggtgaat ccgagcgtcg
      601 gatgttcgcc gaaaacatgt ccgccgcaga ccttgcacca gtgacgaatg ctttgcgggt
      661 tcttgctgta ggacccgatg ttttcggcgc ccttcgtgat cttcacggcc tccggcttcc
      721 acagagtgaa ggcattgaca gggcccgccg cccagtgccg gcaggattcg cagtgacaat
      781 accccatggc catcggctcg ccgctcgcga cgagctcgac ggcgccgcaa aaacaatgac
      841 cgcgatacga tttttcgttg ctcatcgtgg gctccccgtt tggaacgcga atggccgccg
      901 gcctgtatgg gcggagtata aacccgatcc ggaacggccg ccgccgccgc gcttcagacg
      961 tcgcggatgg cagttgcgat cggcagccgc gccgcacgga tcgccgggaa aagtccgccg
     1021 acgagtccca tcagaagcgc gtagatcaca ccggtcgcga gcaggctcgg cgtcacggcg
     1081 aactggaatg cgagcatgcc gccgttgccg atggtcgtcg ccttgaagcc gttgaagagc
     1141 aggtatccga tgaggccgcc gacgatcccg cccgcggcgc cgaggagcag tgcctcgacg
     1201 acgaccgaga gcacgacggg cgagcgcgaa aatccgagcg cgcggagcgt cgcgatttcg
     1261 cgggtgcgcg ccgcgaccgc ggcgtacatc gtgatcagcg cgccgaacac cgcgccgatt
     1321 cccatgagca aggcgatcac gccgccggcg acggtgagaa cgccgtcgag atccttggaa
     1381 gcgttggcga agtacgtgct ttcgcggtcc acgctcacgt tcaactgcgg attgctcttc
     1441 agcgccttct cgaagccgtc gaaggccgcg gccgaagtga gcttgacgta cacgccggag
     1501 aagttgttgc cggcgttgta ggcggcctgc acgtcatgca cgtccgccca cacttccgat
     1561 tcgtgcatgt cgcctccgga tgaaaaaatg ccgaccacct tccattcgtg gtggttccac
     1621 tgaaaggtat tgccgagtcg aaattgcggg aattgctggg acgccgcgcg cccgacgacg
     1681 acctcgtcga gaccggagcg gaacaggcgc ccctggacga tttgcacctt gttccagatg
     1741 tgctcgaagc cggggccgac gccgcggaga gtcacggccg cgagcgtgcc cgagtccttg
     1801 tgcgccatga tcgaggtcac gaggtcgccg agcgcgagcg gacccgccgg cccctgcgcc
     1861 acgcccgggg cctcgccgat tgcacgtacc gtctggagat cggtcatgcc ggggccgtcg
     1921 ttcgagacga tcgcgacgtc gtccgagccg gtgtgggcca gcaccgcggt cagcccgtcg
     1981 cggatcgaga gcagcccgat gaagacgagc accacgccgc agaatccgat caccgcgatg
     2041 acggccggct gcaggcggaa gcgcaccgaa ctcagactga agcggcaggt cgagacggcc
     2101 tcgcgcgccc atacggtgag tgacatgtca ggccctcctc agggcatcgg ccgcgcgcag
     2161 gcgaaacacc tgacgcagcg gcagagcccc ggcaatcaaa ccgaacagga tcatcagcaa
     2221 cactcccagg ccgacggctt ccgcgggcat gccgagggcg ggcaggaccg ccgagaggct
     2281 cccgtacatc gcccgcgcga ggagccaggc ggcggcgagc ccgagcaggc cgccgatcag
     2341 cgtgacgccg accgcttcgg cgagcacgag accggaaacg gtaccgcggc cgaagccgag
     2401 cgccttcagc accgcgagtt ccgaggtccg ctcgcgcacc gcctgcgaca tggtgttgat
     2461 cgtcaccagc aacatgctga aaaacacggc cgcgccgatc acgagcgtca tgaaaccgat
     2521 attgccgaat tcgcggagga aggtctgcgt ttccgcgact tccgactcgg tgcgcgtctg
     2581 cgggaaggag ttcgcgaact tgtcgtcgat cgcgcggctg atctcgggcg ccgcgcgcgg
     2641 atcggagatc cgttcgatgt actggaacac gagatccttg ccggtcgcga tcgaatcgtt
     2701 gaagaagggg tagtgaatca tcacgaaact cttgtagcgg tccggaagat ccgcgtcgta
     2761 gatgccgtcg atcgtgaggt cccaggtgtt cgagccgtcg tccttcgtga gatccttgct
     2821 gcggatcggc acctggtccc cgaccttcca gccgtagcgt ttggcgagca gcgtgccgac
     2881 gatggcgccg gtgcggtcgc cgaaccaggc ttcgcgcgcc tgcggactca agagaatttc
     2941 cgggtacacc ttcaggaacg ccgccgggct caccgccacc acctgcatgt cgttcttttc
     3001 gtcctggtag tagccgtcga aaccggacag ccaggcgacc gtcgtcacac cgggcaccga
     3061 ttcgattttc ggcgcgtagg cgatcgggag cgcacccagc ttcgcgttgg tctggatgcg
     3121 ctgcgcgctc gcaatctcga gcactccgtt catgccatgc tgcatcgcgg cgagcagccc
     3181 gaagagcgcg aacgccgaca tcaccgcgaa cagggtaaag atcgtgcgcg gcttcttgcg
     3241 cttgaggttg ctccacagga gcggccagta tt
//
LOCUS       DASUJG010000357         2099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1570499, whole genome shotgun sequence.
ACCESSION   DASUJG010000357 DASUJG010000000
VERSION     DASUJG010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1570499"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(408..884)
                     /locus_tag="VFX38_07675"
     CDS             complement(408..884)
                     /locus_tag="VFX38_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEX5314763.1"
                     /translation="MRTYDLTPFYRSTVGFDRLFSLLDQANSDGGNGYPPYNIERTGE
                     NAYRISVAVSGFAKEELSIVAKENTLTIKGEKVANENSKDKSEVLYRGIAARAFERVF
                     QLADFVIVKSASLEHGLLHVDLVREIPEAKKPRSIPIGTTSPAPQVVDSSAEKAAA"
     gene            1106..2053
                     /locus_tag="VFX38_07680"
     CDS             1106..2053
                     /locus_tag="VFX38_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5314764.1"
                     /translation="MTLVSIPANPVPENVVAGTLRTPDGAELRFARWAPPAGRKGTVC
                     IFQGRGEQIEKYFETVRDLRDRGFAVATIDWRGQGHSSRRLRDPRKGYVRDFADYEID
                     VETFVQQVVLPDCPPPHFALAHSMGGAVMLRLAHAGKRWFDRMVLSAPMIDLPGRSTT
                     LPVRLLLRAMRVSGQGGRYVLGGNDTLTGTRSFINNPLTSDPVRYARNAAVLEEDPTL
                     GIASPTVAWADTAFRAMNTFRRMYYPSQIRQPILMLAAASDEVVSTSAIEEFAYHLRA
                     GSHLVIAGSKHEILQEQDRYRAQFWAAFDAFVPGTPLFK"
BASE COUNT          371 a          665 c          674 g          389 t
ORIGIN      
        1 catcggttcc gcacgtcgca agtcggggcg tgccgctgcc cgcgccgaaa ccggccgtcg
       61 cgggaaggac gcctgatccg acgccgcagc catcggctgc tgccgccacc agatcgatag
      121 cgcccgcggc gccgacggcg catgcgagcg cgatgccgca gggctcggcg acagtgggcg
      181 aagcgaagtc cgctcccgtg atccggccga cgcaggagat gccgaagatg caagatttgg
      241 agtgattgct tcaatgccgt cattccggcg cgatgcgtag catcgaaccc ggaatctcga
      301 gattccgggt tcacgcttcg cgcgccccgg aacgacagac gacaaaaaac gccccggttt
      361 cccgggacgt ttcgttacgc aaggctacgc gaaaacctgg ttcgcgctca ggcggcagcc
      421 ttctcggccg agctgtcgac cacctgcggg gcaggagagg tcgtgccgat cggaatgctg
      481 cggggcttct tcgcctcggg aatctcgcgc acgagatcga catggagcag tccgtgctcg
      541 agcgacgcgc tcttcacgat cacgaagtcg gcgagctgga agacccgctc gaaggcgcgc
      601 gcggcgatac cgcggtacag cacctcggat ttgtccttgc tgttctcgtt ggcaactttc
      661 tcgcccttga tcgtcagcgt gttctccttc gcgacgatgg agagctcttc cttggcgaag
      721 ccggaaacgg cgaccgagat gcgataggca ttctcgccgg tccgctcaat attgtagggg
      781 ggataaccgt tgccgccgtc agaatttgcc tggtcgagca gagagaaaag gcgatcgaag
      841 ccgacggtgg aacgataaaa cggagtaagg tcgtaggtac gcatcaggta gtcctccatt
      901 gagcgactgt ttcaaatacc cgcccgccat cgggccgggc ttagtctgtg cagcctcaac
      961 gttcggagtt ccgaaacact ggtagcggcc tgcacgaaga tgatatggga gaggcaaaga
     1021 cggcgttcaa gagggcggga tccgagcctt ttttgaccct cccgaccctt gtttttcagc
     1081 gtttttctcc tacccagcgg cccgcatgac gctcgtctcg attcccgcca atcccgttcc
     1141 ggaaaacgtc gtcgccggca cgctgcggac gcccgacggc gcggagttgc gcttcgcgcg
     1201 ctgggcgccg cctgcgggac gcaagggcac cgtctgcatc tttcagggac gtggcgagca
     1261 gatcgaaaaa tatttcgaga ccgtgcgcga tctgcgcgac cgcggcttcg cggtggccac
     1321 catcgactgg cgcggacagg gtcattcctc gcgccgtctg cgcgatccgc gcaaaggtta
     1381 tgtccgcgac ttcgccgact acgagatcga cgtggaaacc ttcgtgcagc aggtggtgct
     1441 gccggattgc ccgccgccgc atttcgcact ggcgcattcc atgggcgggg cggtgatgct
     1501 gcggctggcg catgcgggaa aacgctggtt cgaccgcatg gtgctgtcgg cgccgatgat
     1561 cgatctgccg ggtcgctcca ccacgctgcc ggtgcggttg ttgttgcggg caatgcgtgt
     1621 cagcgggcag ggcgggcgct acgttctcgg cggcaatgac acgctcaccg gcacgcgctc
     1681 cttcatcaat aacccgctga ccagcgatcc cgtgcgctac gcccgcaacg ccgccgtgct
     1741 ggaggaagac ccgacgctcg gcatcgcctc gccgacggtc gcctgggccg ataccgcctt
     1801 tcgcgcgatg aacacctttc gccggatgta ttatccctcg cagatccgcc agccgatcct
     1861 gatgctggcg gccgcgagcg atgaggtggt ctcgacatcc gcgatcgagg agttcgccta
     1921 tcatctgcgc gccggatcgc acctcgtgat cgccggttcg aagcacgaga tcctgcagga
     1981 gcaagaccgc tatcgcgccc agttctgggc cgcgttcgat gccttcgtgc cgggcacgcc
     2041 gctgttcaag tgatgcgggc gaagtaagta ggcgtcaaag tacaattcag tcgtcatgc
//
LOCUS       DASUJG010000358         2206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1570786, whole genome shotgun sequence.
ACCESSION   DASUJG010000358 DASUJG010000000
VERSION     DASUJG010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1570786"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VFX38_07685"
     CDS             complement(<1..761)
                     /locus_tag="VFX38_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEX5314765.1"
                     /translation="MDVSQLLAVGKKHNASDLHLSAGVPPMIRVDGELRRVSQPALEH
                     KDVRDMLYDIMGDRQRKNYEEFLETDFSFEIPGLARFRVNAFNQKSGAAAVFRTIPEG
                     VVTLEDVGSPPVFREIVHEPRGLVLVTGPTGSGKTTTLAAMINYINENRTAHILTIED
                     PIEFVHESKKCLINQRELNRDTLGFSEALRSALREDPDVILVGEMRDLETIRLALTAA
                     ETGHLVFATLHTSSAAKTIDRIVDVFPAGEKEMVR"
     gene            918..1184
                     /locus_tag="VFX38_07690"
     CDS             918..1184
                     /locus_tag="VFX38_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314766.1"
                     /translation="MNRRIGSGNMARALAGGPIAAVWPADALAPTVERLGRARPLPLS
                     IAAGMHTAALEESARDDFRGLVARAFPRARDPCARIREPLRRAG"
     gene            1184..1741
                     /locus_tag="VFX38_07695"
     CDS             1184..1741
                     /locus_tag="VFX38_07695"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX5314767.1"
                     /translation="MSGNGVRALIFVVNVAFSLYIAALYLRILLQRLGADYYNPLVQL
                     VVRASDPLVRPLRRLLPSPGGWDFASFVLAVACAFLNAWLVLLLLGFASAGDLLLRYA
                     GMKLLAVLVNLYIVTILIQALMSWLQPRRYSPLALVLWRLNEPLLAPVRRILPRLGMF
                     DLSALVVLVVLEALSIFLGLPGYLS"
     gene            1850..>2206
                     /locus_tag="VFX38_07700"
     CDS             1850..>2206
                     /locus_tag="VFX38_07700"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX5314768.1"
                     /translation="MAKTGKAAAPATSKPLTKSQIVRYVAEHAELTHGQVADVFESLE
                     RLIKRELGRKGPGVFNLAGLLKVRLATKAATKAREGRNPFTGETIMIKAKPARRTVRV
                     SALKKLKDMAGGAAVPR"
BASE COUNT          355 a          731 c          704 g          416 t
ORIGIN      
        1 ctgcgtacca tttccttctc gccggccgga aacacgtcca cgatccgatc gatggtcttc
       61 gccgcggagc tggtatgcag ggtggcgaag acgagatggc cggtttccgc ggcggtcagc
      121 gcgaggcgga tggtttcgag atcgcgcatt tcgccgacga gaatcacgtc cgggtcctcg
      181 cgcagcgcgc tgcgcagggc ctcggaaaag ccgagggtgt cgcgattgag ctcgcgctga
      241 ttgatcaggc attttttcga ttcgtgcacg aactcgatcg ggtcctcgat cgtgaggatg
      301 tgcgccgtgc ggttttcgtt gatgtaattg atcatcgccg cgagcgtcgt ggtctttccc
      361 gagccggtcg ggccggtgac gagcacgagg ccccgcggct cgtgcacgat ctcgcggaac
      421 accggcggcg agccgacgtc ctcgagggtg acgacaccct ccggaatggt gcgaaaaacg
      481 gccgccgcgc cgctcttctg attgaaggcg ttcacgcgaa agcgcgcgag gccgggaatc
      541 tcgaaggaaa aatccgtttc gaggaactct tcgtagttct tgcgctggcg atcgcccatg
      601 atgtcgtaga gcatgtcgcg cacgtccttg tgctcgagcg ccggctggct gacgcgacgc
      661 agctcgccgt ccacgcggat catcggcggc acccccgccg agaggtgaag atccgaggcg
      721 ttgtgcttct tgccgacggc gagaagttgg gaaacgtcca gcgccatgtg ccactccctt
      781 cgcgaacgct gcgcacttta taaccccaag gtcgtcgcgg cgcaagcctc gcgtcctcgg
      841 acgcaacccc cggcgaggcg ccggcagccg ataaaatgac cgcctcttgc gattgccgcg
      901 acagggaaga gggtggcatg aaccgacgca tcggcagcgg caacatggcg cgggcgcttg
      961 cgggcgggcc gatcgccgcc gtttggcctg cggatgcgct cgccccgacg gtcgagcggt
     1021 tggggcgcgc acggccgctg ccgctctcga tcgcggctgg catgcatacc gcggcgctgg
     1081 aggagtctgc gcgcgacgac ttccgcggtc tcgtcgcccg ggccttcccg cgcgcccgcg
     1141 acccctgcgc gcgaattcgc gaaccattga ggcgcgcggg ctgatgtcgg gcaacggcgt
     1201 gcgcgccctg atattcgtcg tcaatgtggc gttcagtctc tacatcgcgg cgctctacct
     1261 gcgcatcctc ctgcagcgac tcggagccga ttattacaac ccgctggtgc aactcgtcgt
     1321 gcgcgcgagc gacccgctgg tgcggccgct gcgccggctg ctgccctcgc ccggcggttg
     1381 ggatttcgcc tcgttcgtac tcgccgtcgc ctgcgcattt ctcaacgcct ggctggtgct
     1441 cctgctcctc ggctttgcgt cggccggcga tctcctgctg cgttacgccg gcatgaaatt
     1501 gctcgcggtc ctggtaaacc tctatatcgt caccatcctc attcaggcac tcatgagctg
     1561 gttgcagccg cgccgctaca gcccgctcgc gctcgtcctg tggcgtctga acgaaccgct
     1621 gctcgcgccc gtgcgccgga tcctgccgcg actcggcatg ttcgatcttt cggcactcgt
     1681 cgtgctcgtc gtgctcgagg cgctgtcgat cttcctcggc ctgcccggct atctttcctg
     1741 atctgcgttc accgagagca aagagtggga aaaaagctgc aaaacgcctc gcgctgtgct
     1801 acgcttcgca acgcattttc ctacttgatc cactcaatca ggagttccca tggcgaagac
     1861 aggcaaggca gcggcccccg cgaccagcaa gccgcttacc aaatcccaga ttgttcgtta
     1921 cgtcgccgag cacgccgagc ttacccacgg tcaggtggcc gacgtctttg aatcgctgga
     1981 aaggctgatc aagcgcgaac tcggtcgcaa gggccccggc gtcttcaatc tcgccggcct
     2041 gctcaaggtc cgtctggcca ccaaggcggc caccaaggca cgcgagggca ggaatccctt
     2101 taccggcgag acgatcatga tcaaggcaaa gccggcgcgg cgcaccgtgc gggtgagcgc
     2161 gctcaagaaa ctgaaagaca tggccggagg ggccgccgtt ccccgg
//
LOCUS       DASUJG010000359         2068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_960440, whole genome shotgun sequence.
ACCESSION   DASUJG010000359 DASUJG010000000
VERSION     DASUJG010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960440"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="VFX38_07705"
     CDS             complement(<1..691)
                     /locus_tag="VFX38_07705"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEX5314769.1"
                     /translation="MKYTGVSTNILDAIGDTPLVVVDGVYAKLEYLNPSGSIKARIAK
                     YMIEKAEAEGLLKRGDTIVEATSGNTGNALSMVAAVKGYRMLVVMPEGLSSERVAISR
                     AFGAEILFVGSFHVNEALAKAKELGKLPGHFCPSQFESDSNVEENREILGPEILAQLP
                     EGAIPDALVMGVGTGGTLAGVGQAFRKVNPAVRLFAMEPAESCTLLCGEIGKHQIEGI
                     SDGFVPGIFARH"
     gene            complement(768..1544)
                     /locus_tag="VFX38_07710"
     CDS             complement(768..1544)
                     /locus_tag="VFX38_07710"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX5314770.1"
                     /translation="MQEKRDTVRIAAGPEPADVIPRLRESVVAILRLHPNARSHAKKD
                     GGTFEGSIVGTAFCVLANRYLLTAHHILAGGAKRLERDRFVAFAVPRNGRDAVHFPVT
                     GFPLERPDLDFAVLAIEPGATAGFALPALALGFEEERDGAAVLSLGFPAPKVVKLGVD
                     PAGNYRGGEFFLKSHANTGIVSAFYTVGGIPFYELNIGWHHGESGGPIVTLSDPPVAF
                     SMMQHYRNIQSPHGVMAGPHRGRSFAPIRDELAALGVHPA"
     gene            1685..>2068
                     /locus_tag="VFX38_07715"
     CDS             1685..>2068
                     /locus_tag="VFX38_07715"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5314771.1"
                     /translation="MRPAAAVTAASLAGRSVFRHPGVAQFLGARLAATIASQMQAVVV
                     GWQVYHISHSPLALGFVGLAQFVPMALFILPAGDFADRLNRKHLLVLGWALQALASVL
                     LAALTFSHFPGLLPFYAVLVLFGAAR"
BASE COUNT          310 a          671 c          708 g          379 t
ORIGIN      
        1 gatggcgggc gaagattccc ggcacgaagc cgtcggaaat tccttcgatc tggtgcttgc
       61 cgatctcgcc gcacagcagc gtgcaggact cggccggttc catcgcgaac agacgcacgg
      121 cggggttgac cttgcggaac gcctggccca cgccggcgag cgtaccgccg gtgccgaccc
      181 ccatgacgag cgcatcgggg atcgcgccct cgggcagctg cgcaaggatc tcggggccga
      241 ggatttcccg gttttcctcg acgttcgagt cggactcgaa ctggctgggg cagaagtggc
      301 cgggcagctt gccgagttcc ttcgccttgg cgagcgcctc gttcacgtga aagctgccga
      361 cgaagagaat ttccgcgccg aatgcgcgcg agatcgcgac ccgctcgctg gagagtccct
      421 ccggcatcac cacgagcatg cggtagccct tgaccgccgc gaccatcgag agcgcgttgc
      481 cggtgttgcc gctcgtcgcc tccacgatcg tgtcgccgcg cttgagcagg ccctcggcct
      541 cggccttctc gatcatgtat ttcgcgattc gcgccttgat cgaacccgag gggttgagat
      601 attcgagctt ggcgtagacg ccgtcgacta cgaccagcgg cgtgtcgccg atcgcatcga
      661 gaatgttggt cgaaaccccg gtgtatttca tcgcttctgg actcgtctcg gtgcgcgccg
      721 cgagcatacg ccgaagcacc gcttcctgcc aagtcccgcg gcgggtctca cgccggatgc
      781 acgccgagtg cggcaagttc gtcgcggatc ggggcgaagg agcggccgcg atgcgggccc
      841 gccatcacgc cgtgcggtga ctggatgttg cggtaatgct gcatcatcga aaaggcgacc
      901 ggcggatcgc tcagggtgac gatcggcccg ccgctttcgc cgtggtgcca gccgatgttg
      961 agttcgtaaa acgggattcc accgacggta tagaaggcag agacgatgcc cgtgttcgca
     1021 tggctcttga ggaagaattc gccgccgcga taattgcccg cgggatccac gccgagcttc
     1081 acgactttgg gtgcgggaaa gccgaggctc agcacggcgg cgccgtcgcg ctcctcctcg
     1141 aatccgagcg cgagcgccgg gagcgcgaag ccggccgtcg cgcccggttc gattgcgagc
     1201 acggcgaaat cgagatcggg gcgttcgagc gggaagccgg tgacggggaa gtgcacggcg
     1261 tcgcggccgt ttcgcggcac ggcgaaggcg acgaagcgat cgcgctcgag ccgtttggcg
     1321 ccccccgcca ggatgtgatg ggcggtgagg aggtaacggt tcgcaagcac gcagaacgcg
     1381 gtgccgacga tgctgccctc gaaggtccca ccgtccttct tggcgtgcga gcgcgcgttc
     1441 gggtgcaggc gcaggattgc gacgaccgac tcgcgcagac gggggatgac gtccgccggt
     1501 tccggtccag cggcgatgcg gactgtatcg cgtttctcct gcatggcgga aaccttagca
     1561 tggtggcgcc gcgatcatcg cgcgggcgcg gtagccgaat gcgaaaatag cgcccgatgt
     1621 tcaacctccg cgccgatccg gcggtcggcg gcttgccgtg cagcccgcgg agggcggctt
     1681 ccccgtgcgg cctgccgccg ccgtgaccgc agcgtcgctc gcgggtcgtt cggtattccg
     1741 ccatccggga gtcgcgcagt ttcttggcgc gcgccttgcg gcaaccatcg cctcgcagat
     1801 gcaggccgtc gtggtgggtt ggcaggtgta ccacatctcg cacagcccgc tcgcgctcgg
     1861 cttcgtcggc cttgcccaat tcgtaccgat ggcgcttttc atccttccgg ccggcgactt
     1921 cgccgatcgg ctcaatcgaa agcatcttct ggttctcggc tgggccttgc aggcgctggc
     1981 gagcgtactt ctcgccgcgc tcacgttctc gcattttccg gggcttctgc cgttttacgc
     2041 ggtactggtg cttttcggcg cggcgcgc
//
LOCUS       DASUJG010000360         3619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1447992, whole genome shotgun sequence.
ACCESSION   DASUJG010000360 DASUJG010000000
VERSION     DASUJG010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447992"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VFX38_07720"
     CDS             complement(<1..456)
                     /locus_tag="VFX38_07720"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEX5314772.1"
                     /translation="MKGNLLFAATGALLGMLPAAVLAAAPDIVVYQWTDARGTTHFSD
                     APRKDGPAKRLLLATPPPADRSAQAANRAWLAELDRDTEARLAREAAQRQAETRREAA
                     LAQERRQQAVAEQAVQRVPILYSGRRQIHRRPRRVHRGPWRFSVGAVASF"
     gene            complement(507..1412)
                     /locus_tag="VFX38_07725"
     CDS             complement(507..1412)
                     /locus_tag="VFX38_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314773.1"
                     /translation="MAGSALRDNAERSRFEENDMTPRILPVAAAAFSLALFLVPAAFA
                     AEAASPADPTLAQAVGGSWRTPDYVQRDQYRHPLPVLRFFGIRPDMTVVELAPGGGWW
                     TEILAPYLKAEGQLIETVPPENAKSHMGGMSGRFLAKLKADPALYGKVKTVPFAPPQV
                     VRLGPPDSADLVLTFRNLHDWVNDDSAKAVFKAAYEVLKPGGVFGVTDHRALPFANGN
                     EASKALHRLPEDFVIQMGLDAGFQLAGVSEVNANAKDPMTINIHHLPPDLADDTAAEK
                     KKYLAIGESDVFVLKFVKPGAQAGE"
     gene            1500..>3619
                     /gene="recC"
                     /locus_tag="VFX38_07730"
     CDS             1500..>3619
                     /gene="recC"
                     /locus_tag="VFX38_07730"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HEX5314774.1"
                     /translation="MLHIHFGNRIERLADELADTLKGAPPADPLAPETVVIGHAGMDE
                     WLKRALAERLGVAANLDFVQPAAFVWRLLRERDPKLLTGSPLDRAPLVWRLYAALGEH
                     EPAAEVANYLRGKDELARFELADALAAVFEQYQLYRPQMILDWETNPAAGGDAAWQAA
                     LWRRVAKGAGPNRARLYRDFLKAADSGKQPNSHPRVSVFGITALAPVHLEILAALARD
                     RDVSLYAPNPSRAYWGDIESEKRLARWALTDPGRGEYATSGHPLLSALGMQTRDFIEL
                     LIGLNDEGPEEHFEEPAGESALARLQRDILDLVEQPETIAADSADESLRVHVCHSRRR
                     EVEVLHDALLAAFEADPTLGPEEILVMAPNMDDYAAHVAAVFGAAPDERHIPWSLAER
                     SLRAEHPLAEALLTLLDLPESRFKASEVLGLLDLPAVARKLELDEQALEAIHRWTGEA
                     GIRWAWDGAHRAAFDLPAEDAFTWRFGLDRLLAGYAFAPDEDALWEGIAPWTGLEGQA
                     ARALGSLCACLARLDGWRKSLAEARSLDEWVTAIRELLTLFAPESPDEQTAMAALRQA
                     LSDLDTNRELAGFREPVPRNVVRAALAAGLALPRHPRPFLSGRVTFCALAPMRSIPAK
                     LVWLLGMSEEDFPRRGRAPGFDLIAAHPERGDRARQTEDRALFLEAILSARGAFHASY
                     IGRSERDNGELPASLVVNMLLDVT"
BASE COUNT          574 a         1273 c         1170 g          602 t
ORIGIN      
        1 aaagctcgcg actgcgccga ccgaaaacct ccacggcccg cgatgaacgc ggcgcggacg
       61 acgatgaatt tggcgccttc ccgagtagag tatgggaacg cgttgcacgg cttgctccgc
      121 gacggcctgc tgccttcgct cttgcgcgag cgccgcctcg cgccgcgtct cggcctgcct
      181 ctgcgccgcc tcgcgggcga gccgcgcctc ggtgtcgcga tcgagttcgg cgagccaggc
      241 ccggttcgcc gcctgcgcgc tgcgatcggc aggcggcgga gtcgcgagca ggaggcgttt
      301 cgccggaccg tccttgcgcg gcgcatcgct gaaatgcgtc gttccccggg cgtcggtcca
      361 ttggtacacc acgatgtccg gagccgcggc aagcactgcg gccggaagca tcccgagcag
      421 agcgcccgtc gccgcgaaga gcaaattccc tttcaatcgc ctgccttgca tcgttcgtta
      481 cttcgaccgt tgcgagcccg cggcgttcac tcgccggcct gcgcccccgg cttcacgaac
      541 ttgagcacga acacgtcgct ttcgccgatc gccagatatt tcttcttctc cgcggccgtg
      601 tcgtcggcca gatccggcgg cagatgatga atgttgatcg tcatcgggtc cttcgcgttc
      661 gcgttcacct cggatacccc ggcgagttgg aaacccgcat cgagccccat ctgaatcacg
      721 aagtcctcgg gcaggcgatg cagtgccttc gaggcttcgt tgccgttcgc gaacggtagc
      781 gcgcgatgat cggtgacgcc gaaaacgccg cccggcttga gtacttcgta ggccgccttg
      841 aacaccgcct tggccgagtc gtcgttgacc cagtcatgca gattgcggaa ggtcaaaacc
      901 agatcggccg agtccggcgg cccgagcctg acgacctgcg gcggcgcgaa gggcacggtt
      961 ttcaccttgc catacagcgc ggggtcggcc ttgagcttgg cgaggaaacg cccgctcatc
     1021 ccgcccatgt gagacttcgc attctccggc ggcacggtct cgatcaactg gccctcggcc
     1081 ttcagatagg gagcgaggat ttcggtccac cagccgccgc cgggcgcaag ttcgaccacc
     1141 gtcatgtccg gccggatgcc gaaaaaccgg agcactggaa gcggatggcg gtattgatcg
     1201 cgctggacgt agtcgggcgt gcgccagctt ccgccgacgg cctgcgcgag cgtcggatcg
     1261 gccggtgaag cggcttccgc cgcgaaggcc gcagggacca ggaacagcgc cagcgagaag
     1321 gctgccgcgg cgaccggaag aatcctcggg gtcatgtcgt tctcctcgaa acgggatcgt
     1381 tccgcattgt cgcgcagcgc cgagcccgcc acaaggagcc tccgccgcag cttgtcgcgg
     1441 gttctgcgac cgcacccggg ctaccatgat gctcgcggat tcgaaccggg tggcaaccct
     1501 tgctgcacat tcacttcggc aaccgcatcg agcggctcgc ggatgaactg gccgacacgc
     1561 tcaagggcgc gccgcccgcc gacccgctcg cgccggaaac cgtcgtcatc ggccacgccg
     1621 gcatggacga gtggctcaag cgcgcgctcg ccgagcggct cggcgtcgcc gcgaatctcg
     1681 acttcgtgca gcccgcggcc ttcgtgtggc ggctactccg cgagcgcgat ccgaaactgc
     1741 tgaccggttc gccgctcgat cgtgcgccgc tcgtgtggcg tctttacgcc gcgctcggcg
     1801 aacacgagcc ggcggcggaa gtcgcgaact atctccgcgg caaggacgaa ctcgcccgct
     1861 tcgaactcgc cgacgcgctg gcggcggtgt tcgagcagta tcagctctac cgtccccaaa
     1921 tgattctcga ctgggagacg aatccggctg ccggcggcga tgccgcctgg caggcggcgc
     1981 tctggcggcg ggtcgcgaag ggagccgggc cgaatcgcgc ccggctttac cgggactttc
     2041 tgaaagctgc cgattccggc aagcagccaa actcgcatcc tcgcgtttcg gtcttcggca
     2101 tcaccgcgct cgcgccggtt catctcgaaa ttctcgccgc cctcgcacgc gaccgcgacg
     2161 tttccctcta cgcgccgaat ccgtcgcggg cctactgggg cgacatcgaa agcgaaaagc
     2221 ggctcgcccg ctgggcgctc accgatcccg gacgtgggga atacgccacc agcgggcacc
     2281 cgctcctctc tgcgctcggg atgcagaccc gcgacttcat cgaacttcta atcggtttga
     2341 acgacgaggg gcccgaggaa catttcgagg agcctgcggg cgaaagcgcg ctcgcacgat
     2401 tgcagcgcga catcctcgat ctcgtcgaac aacccgaaac catagcggca gattcggcgg
     2461 acgaatccct gcgggtgcac gtctgccaca gccgccggcg cgaggtcgag gtgctgcacg
     2521 atgcgctgct cgcggccttc gaggcggatc cgacgctggg acccgaagag atcctcgtga
     2581 tggcgccgaa catggacgac tatgccgcgc acgtcgccgc ggtgttcggg gcggcgccgg
     2641 acgagcggca cattccctgg agcctcgccg agcgcagcct gcgcgccgaa catccgctcg
     2701 cggaagccct gctcacgctg ctcgatcttc ccgaatcgcg cttcaaggca agcgaggtgc
     2761 tggggcttct cgatctcccc gccgtcgcgc ggaaactcga actcgacgag caagcgctcg
     2821 aggcgatcca tcgctggacc ggcgaagcgg gcatccgctg ggcctgggac ggcgctcatc
     2881 gcgccgcgtt cgatctgccc gcggaggacg ccttcacctg gcgtttcggt ctcgatcggc
     2941 tcctcgccgg atacgccttc gcgcccgacg aggacgcgct gtgggagggc atcgcgccct
     3001 ggacggggct cgaaggtcag gcggcgcgcg cgctcggttc tttgtgtgcc tgtcttgcgc
     3061 gactcgatgg ctggcgcaag tcactcgccg aagcgcgatc gctcgacgaa tgggtcaccg
     3121 cgatccgcga actcctcacg cttttcgcgc ccgaatcgcc cgacgaacag acggcgatgg
     3181 ccgcgcttcg ccaagcgcta agcgatctcg atacgaatcg cgaactcgca ggtttcagag
     3241 aacccgtgcc gcgcaatgtc gtgcgcgccg cgctcgccgc cggtctcgcg ctgccgcgcc
     3301 atccgcgtcc cttcctttcc gggcgagtca ccttctgcgc gctcgcgccg atgcggagca
     3361 ttcccgcaaa gctcgtgtgg ctgctcggca tgagcgaaga agacttcccg cggcgcggac
     3421 gcgcgccggg cttcgatctc atcgccgcgc atccggagcg cggcgaccgc gcccgccaaa
     3481 cggaagaccg cgcgctcttt ctcgaagcaa tcctgagcgc ccgcggtgcc ttccacgcga
     3541 gttacatcgg ccgcagcgaa cgcgacaacg gggagctgcc cgcctcgctc gtcgtcaaca
     3601 tgttgctcga tgtcaccga
//
LOCUS       DASUJG010000361         2317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_714176, whole genome shotgun sequence.
ACCESSION   DASUJG010000361 DASUJG010000000
VERSION     DASUJG010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714176"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="VFX38_07735"
     CDS             <1..956
                     /locus_tag="VFX38_07735"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF036491.4"
                     /note="catalyzes the formation of agmatine from arginine
                     in putrescine and spermidine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HEX5314775.1"
                     /translation="YAEVIVRTLAEVAGEAGQPVPAIVSESGRALTAHHAVLVTKVIE
                     TERAPDAPAAAPAEDAPAALRNLWEAYEYSGEGNPQEIHADAAYEISEIHSLFSHGLV
                     SLAERAHAEAMYAALCRRVLARINPTRASDWEFADHLVEKLADRMFINLSIFQSLPDA
                     WAIDQVFPIVPLSRLDEVPTRRAILHDLTCDSDGRIEAYVDNEGVEKTLAVHEPDGNP
                     YLLGIFMVGAYQEILGDMHNLFGDTNAVNVLTRRDGYIIRDTREGDSAQVMLRYVHIP
                     PRALARTWRRRLAKSGLDSSRQAELAMLLERGLKAYTYLDR"
     gene            953..2026
                     /locus_tag="VFX38_07740"
     CDS             953..2026
                     /locus_tag="VFX38_07740"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX5314776.1"
                     /translation="MNDDPVALAPPAATGFLPDLCSPVATLPLVLIAALVALALAVAR
                     AAGPGFWPEFGRLLLFCEVLTLASAAVLCLLRRVLSGARPVLLAAVALVALVATDWAV
                     AEGAWFLLATLAVPLPGGPQARFWWVLRIVVVAAIVDALVLRYLYLGASWRENVRREA
                     TSRLAALTARIRPHFLFNTLNTAIALVAERPQDAERSLEDLAELFRASLADQGARIAL
                     AEELAVVRRYLAIETLRLGSRLAVHWRLAPLVERALVPGFILQPLVENAVTHGIEPAG
                     AGGSVEIRAERAGRMLELAVLNSVDAASGGAGYGIGLSGIRARLALVYGGDATLAVER
                     SPGNFGVRLRCPWEECDADPDRR"
     gene            2004..>2317
                     /locus_tag="VFX38_07745"
     CDS             2004..>2317
                     /locus_tag="VFX38_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5314777.1"
                     /translation="MQILIADDEAPARSRLRRMIGELAAGEIVGEAASGREALALAER
                     RRPDVVLLDIRMPEGDGIAMARALTKLEPPPAVVFVTAFADRALPALEAGATAYLVKP
                     VSR"
BASE COUNT          329 a          800 c          783 g          405 t
ORIGIN      
        1 tctacgccga ggtgatcgtg cgcacgctcg ccgaggtcgc gggcgaggcg gggcagccgg
       61 tgcccgcgat cgtttccgaa tccgggcgcg cgctcaccgc gcatcacgcg gtactcgtca
      121 ccaaggtgat cgaaaccgaa cgcgcgcccg acgcgccggc cgcggctccc gcggaggacg
      181 cgccggcggc gctgcgcaat ttgtgggaag cctacgagta ctcgggcgag ggcaacccgc
      241 aggaaatcca cgccgatgcc gcctacgaga tcagcgagat tcattcgctc ttcagccacg
      301 gactcgtgtc gctcgcggag cgcgcgcatg ccgaggcgat gtacgccgcg ctctgccggc
      361 gggtgctcgc tcgcatcaat cccacgcgcg cctcggactg ggagttcgcc gatcatctcg
      421 tcgagaaact cgccgatcgc atgttcatca atctttcgat cttccagtcc ctgccggatg
      481 cctgggcgat cgatcaggtc tttccgatcg tgccgctctc gcgtctcgac gaggtgccga
      541 cgcggcgcgc gattctccac gatctgacct gcgattccga cgggcgcatc gaggcctacg
      601 tggacaacga gggagtcgag aagacgctcg cggtgcacga gccggacggc aatccgtatc
      661 tgctcggaat cttcatggtc ggcgcgtatc aggaaattct cggcgacatg cacaatctgt
      721 tcggcgacac caacgcggtc aatgtgctca cgcgccgcga cggctacatc atccgcgaca
      781 cccgcgaggg tgacagcgcg caggtgatgc tgcgctacgt gcacattccg ccgcgcgcgc
      841 tcgcgcgtac ctggcggcgg cggctcgcca aaagcgggct cgattcctcg cgccaggcgg
      901 agttggcgat gctgctcgaa cgcggtctca aggcctacac ctacctcgat cgatgaacga
      961 cgatccggtg gcgctcgctc caccggcggc cacggggttt ctgccggatc tgtgctcgcc
     1021 ggtcgcgacc ctgccactcg tcctcattgc ggcgctcgtc gccctcgcgc tcgcagtggc
     1081 gcgggccgcc ggtccgggat tctggccgga gttcggccgg ctgctccttt tctgcgaagt
     1141 tctgacgctt gcgagcgcgg ccgtactctg cctgctgcgc cgcgtgcttt ccggcgcgcg
     1201 acccgtcctg ctcgcggcgg tcgccttggt ggcacttgtc gcgacggact gggccgtcgc
     1261 ggaaggcgcc tggttcctgc tcgcgacgct tgccgttccc cttccgggcg gcccgcaggc
     1321 gcgattctgg tgggtgttgc gtatcgtggt ggttgccgcg atcgtcgatg cgctcgtgct
     1381 gcggtatctc tatctcggcg cgagctggcg cgagaacgtg cgccgcgaag cgacgagccg
     1441 tcttgccgcg ctcacggcac gcatccgtcc gcattttctc ttcaacacgc tcaacaccgc
     1501 gatcgcgctc gtcgccgaac ggccgcagga tgccgagcgc agtctcgaag atctcgccga
     1561 actctttcgc gcgagcctcg cggaccaagg cgcgcgcatc gcgctcgcgg aagaactcgc
     1621 ggtcgtgcgc cgctatctcg cgatcgagac gttgcggctc ggctcgcgtc tcgccgtcca
     1681 ctggcggctc gcgccgctcg tggagcgcgc gctcgttccg ggtttcatcc tgcaaccgct
     1741 ggtggagaat gccgtgacgc acggaatcga gcccgcgggc gcgggaggat cggtcgagat
     1801 ccgcgccgag cgcgccgggc gcatgctcga actcgccgtg ctcaattcgg tcgacgccgc
     1861 gagcggcgga gcaggctacg gcattgggct ttcgggcatc cgcgcccggc tcgcgcttgt
     1921 ctacggaggg gacgcgacgc tcgccgtcga gcgttccccc ggaaatttcg gcgtgcggct
     1981 gcgttgtccc tgggaggaat gcgatgcaga tcctgatcgc cgatgacgag gcccccgcgc
     2041 gttcgcgtct gcgccggatg atcggcgaac tcgccgccgg ggaaatcgtc ggcgaggcgg
     2101 cgagcgggcg cgaagcactc gcgctcgccg agcgccggcg tcccgatgtc gtgttgctcg
     2161 acatccgcat gccagaaggc gacggaatcg caatggcacg cgcattgacg aaactcgaac
     2221 cgccgcccgc cgtggtgttc gtgacggcat tcgccgatcg agcgctgccg gcactcgagg
     2281 cgggagccac ggcttatctc gtcaaaccgg tgagccg
//
LOCUS       DASUJG010000362         2690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1572562, whole genome shotgun sequence.
ACCESSION   DASUJG010000362 DASUJG010000000
VERSION     DASUJG010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1572562"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..320)
                     /locus_tag="VFX38_07750"
     tRNA            complement(245..320)
                     /locus_tag="VFX38_07750"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(285..287),aa:Asn,seq:gtt)
     gene            complement(358..1275)
                     /gene="galU"
                     /locus_tag="VFX38_07755"
     CDS             complement(358..1275)
                     /gene="galU"
                     /locus_tag="VFX38_07755"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEX5314778.1"
                     /translation="MKVQPIRKAVFPVAGLGTRFLPATKANPKEMLPIVDKPLIQYAV
                     EEAVAAGAELLIFVTGRNKRAIEDHFDRAPELEETLERNSRFEQLKLVQDILPAHVAC
                     IHIRQAEALGLGDAVSCAEAVIGDEPFFVILADDLIAGDGEYCLQQMVAAYRENPGSI
                     LAVQRVDPALTASYGILETEPAEGRLRHVRAMVEKPQPEDAPSNLAVVGRYILTPKVL
                     ERLHGLGRGAGGEIQLTDAIASVLGEEPVYSYEFQGIRYDCGSKLGYLKATVAFALQH
                     PELAGPFAEWLDTGHLPPSPMNGGSGAKV"
     gene            complement(1316..2497)
                     /locus_tag="VFX38_07760"
     CDS             complement(1316..2497)
                     /locus_tag="VFX38_07760"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5314779.1"
                     /translation="MRAEDIALLAALVVAATGAAGILWAWLRERHRRPPGQRFTPEYF
                     RALSLLLDNQMDRALEVFMELADRDEDTVEFHFALGSLFRRKGELERATLIHQNLIAR
                     ESLSREQRDLALKELGQDYLRAGLYDRAERIFLQLLDLKAHQEFAARQLIMIYEQQQD
                     WAQAAALRRRLERITGSDEAQVVAQYCCEMAESAAARGDRAAAVEALAEARRYARELP
                     RVRLLTARAAVERGEPREAVHEYQQLLEGDPGLAELAMPPLAAIFPNGARARDFDSSV
                     SRALASAEQARTPIAAVGFKYVHLRSPAVIRAIEGELTQLISPYSDHEIAPLMDSHEA
                     RATLIPLLSGWVGQRSLYQCRVCGFRAHELYWHCPGCRGWDTMRMRQDVFPLPGIEPT
                     R"
     gene            complement(2487..>2690)
                     /locus_tag="VFX38_07765"
     CDS             complement(2487..>2690)
                     /locus_tag="VFX38_07765"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HEX5314780.1"
                     /translation="HYLLGRVRLPLAAALAGALVVGAIIAALALLPSVLAARARARRL
                     RGRLRAAAEKQESLPPTSFHDAR"
BASE COUNT          478 a          858 c          916 g          438 t
ORIGIN      
        1 cggacaggcg ttgcgccggg gcgcaggcat cgagcgcgtt gcgccaatcc gcttccgcga
       61 gcggacggcg ggcgagaatg cgccgatcgc gccaggctcg aagccctgcg atcatcgccg
      121 gaccatgttc gtgttcatct cgacctgcgc cgagcgtcgc atcgcgccct catcacgccc
      181 ggtgaaaggc gatcggcagg gcttcggaaa ctcggggacg cgcagttgca gctttcgatt
      241 taattggctc cccgggccgg actcgaacca gcgaccaacg gattaacagt ccgccgctct
      301 accaactgag ctaccgggga acacaacgtc ctattttatg gggctgcgcg ggtgcggtca
      361 aaccttcgcg ccggaaccgc cgttcatggg ggaaggcggc aaatgacccg tatcgagcca
      421 ttcggcgaag gggcccgcga gttccggatg ctgaagcgcg aaggcgacgg tcgccttgag
      481 atacccgagc ttgctgccgc agtcgtagcg gattccctga aactcgtagc tgtagaccgg
      541 ctcctcgccg aggacgctcg cgatcgcgtc ggtgagctgg atttcaccgc ccgcgccgcg
      601 gccgaggccg tgcaggcgct ccagcacctt gggggtgagg atgtagcggc cgacgaccgc
      661 gagattggag ggagcatcct ccggttgcgg tttttcgacc atggcgcgaa cgtggcgcag
      721 gcgcccttcg gccggttcgg tctcgagaat cccgtagctt gcggtaagcg cggggtccac
      781 gcgctgcacg gcgagaatcg atccgggatt ttcgcggtag gccgcgacca tctgctgcag
      841 gcaatattcg ccgtcgccgg cgatcaggtc gtcggcgaga atcacgaaga agggttcgtc
      901 gccgatcacg gcttcggcgc aggacactgc gtcgccgagt ccgagcgctt cggcctggcg
      961 gatgtgaatg caggcgacat gggcgggaag aatgtcctgc acgagtttga gctgttcgaa
     1021 tctggagttt ctttcgagcg tctcctcgag ttccggcgcg cggtcgaaat gatcctcgat
     1081 ggcgcgcttg ttgcgcccgg tcacgaagat gagcagctcc gccccggcgg ccaccgcctc
     1141 ctccaccgcg tattgaatga gcggcttgtc cacgatcggc agcatttcct tgggattggc
     1201 cttggtcgcc ggcaggaacc gcgtgccaag tcccgccacc gggaaaaccg ccttgcgtat
     1261 gggttgcacc ttcatgaatt tcaccaaatt cccgcaaaaa gtataaagga agcgctcagc
     1321 gcgtcggctc gatgcccggg agcggaaaga catcctggcg catgcgcatc gtgtcccagc
     1381 cccggcagcc gggacagtgc cagtaaagct catgcgcccg gaacccgcag acccggcatt
     1441 gatagagcga acgctgaccg acccagcccg aaagaagcgg aatcagcgtg gcacgtgcct
     1501 catgcgaatc catcaacgga gcgatttcgt gatcggagta gggcgagatc aattgcgtga
     1561 gttctccctc gatcgcgcgg atcaccgcag gcgaacgcaa atgcacgtac ttgaatccga
     1621 cggcggcgat cggcgtgcgc gcctgttctg cggaggcgag cgcacggctc acactggagt
     1681 cgaaatcgcg ggcacgggcg ccgttgggaa agatcgcggc aagcggcggc atcgcgagtt
     1741 cggcgaggcc gggatcgccc tcgagcagtt gctggtactc gtgcaccgcc tcgcgaggtt
     1801 cgccccgttc cacggcggcg cgcgcggtga gcaggcgcac gcgcggcaac tcgcgcgcgt
     1861 agcgcctcgc ctcggcgagc gcctcgacgg cagccgcgcg atcgccgcgg gccgcggcgg
     1921 actcggccat ttcacagcaa tactgcgcga ccacttgggc ctcgtcgctt ccggtgatgc
     1981 gctcgagacg tcggcgcagg gctgcggcct gcgcccagtc ctgctgctgt tcgtaaatca
     2041 tgatgagctg gcgcgccgca aattcctgat gggccttcag atcgagaagt tgcagaaaaa
     2101 tccgctcggc gcgatcgtag agaccggcgc ggagataatc ctggccgagt tccttgaggg
     2161 cgagatcacg ctgctcgcgc gagagcgatt cgcgggcgat gaggttctga tgaatcagcg
     2221 tcgcgcgttc gagttcgccc ttgcgtcgaa agaggctgcc gagcgcgaag tgaaactcga
     2281 cggtgtcttc gtcgcgatcg gcgagttcca tgaacacctc gagcgcccga tccatctggt
     2341 tgtcgaggag gagcgacagc gcgcggaagt actccggcgt gaaacgctgg ccgggcggac
     2401 gtcggtgccg ctcgcgcagc caggcccaca agatgccggc ggcgcccgtc gccgccacga
     2461 cgagcgccgc caggagcgcg atatcttcag cgcgcatcgt gaaacgatgt cggcggcaac
     2521 gactcctgct tctccgcggc ggcgcgaagc ctgcctcgca ggcgccgggc acgggcgcgc
     2581 gcggcgagca cagacggcag cagcgcgagc gccgcgatga tcgcacccac gacgagcgcg
     2641 cccgcgagcg ccgccgcgag cgggagtcgc acacggccca ggagatagtg
//
LOCUS       DASUJG010000363         5882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_349723, whole genome shotgun sequence.
ACCESSION   DASUJG010000363 DASUJG010000000
VERSION     DASUJG010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5882
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349723"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..1390
                     /locus_tag="VFX38_07770"
     CDS             68..1390
                     /locus_tag="VFX38_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5314781.1"
                     /translation="MRRLSVVAVLLLPLAAFAANTAPANASAATFAMAAPALDAYVGS
                     YELAPGFVLTISRRGEQLYAQATGQGAAPIYESAKDQFFYKVVNAQISFERDAAGNVT
                     GLVLHQNGDHPAPRMASAASAVTAMAPASATNWIAAAAVGTTTLPITVTRDGFVLKGV
                     LDLPAGKGPFGVVDIIPGSGPVSLNGNDGPITYSPYRKLAAALVEAGWAVARVAKRGM
                     APSTGNGNDVVFSDQLADNLAIVAALRKNPHINPRLIVVAGHSLGGLIAPKLATETKL
                     AGLILLEAPGEAMSRISTSQALAMSRQAGASATKLKAIAAEQNQFYAAVAKAPPGQSV
                     RFAGKTLQPDAVQLFKSWYAQKPLATAREVTIPVLVVQGGLDFNVPPGNGKRLVKALP
                     HATLLLLPRMGHALDDADCRCVRQLDTGKDATLAPGLAAGIVRWLQAL"
     gene            complement(1427..2680)
                     /locus_tag="VFX38_07775"
     CDS             complement(1427..2680)
                     /locus_tag="VFX38_07775"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX5314782.1"
                     /translation="MPMRRALIAVCAIAALAGTAAARASGGCANEKDVVDPIVQPHVY
                     AGGAATATQAVGMIVGVWLDGEPCYYGYGTTKLGEQSTPTARTLWEMGSNTKTFTATM
                     LALHQLAGRIRVHDPVDFSEVPCVIAESTSVCYEPTGGMEALTYFELATFTGGLYDDP
                     PNYKFAAAENYTQADFIKAFDVWPAPRNGLPAPNFYSNSSYGILGQVLMSFDGYTDFS
                     DPDAFRANFAQWIGRAILRPLGMRCTNPDPDAMPQACNATARMASGYHLRGDHYEIES
                     SPWPWVPWGPAGSLRSSAADMLRYLGAYLGNRIVAGRSVPWPLTATMTVARRLTAVPV
                     DPLPAGAAFATPRQGYAWVVTPPRPGYDLRFSKDGETHDFSSCVVGVPKKNVGVVIIA
                     NIAMHPGAIPCHIATEIVEQTPPPN"
     gene            complement(2746..3567)
                     /locus_tag="VFX38_07780"
     CDS             complement(2746..3567)
                     /locus_tag="VFX38_07780"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylarginine dimethylaminohydrolase family
                     protein"
                     /protein_id="HEX5314783.1"
                     /translation="MPARHKILMCRPDHFTVSYNINPWMDGEEGEVSRGRALAQWQAL
                     RDVLARVAEVELIPAQPQLPDMVFTANAGVVNGRRVIVSRFSPPERRGEEPHFRDWFA
                     SRGFELVAWPEELVFEGAGDALLDRGGPWVWAGYGQRTALAAHERLAAFYPERETVSL
                     RLVDPYYYHIDTCLAPLEGGYLLYYPEAFDEEGRAAIERRVAPERRIAVNAAEADALS
                     CNAVNLDRHVFLNNPSERLRNVLETAGFEVTGVDVSEFMKSGGAAKCLVLRLDEP"
     gene            complement(3636..5318)
                     /locus_tag="VFX38_07785"
     CDS             complement(3636..5318)
                     /locus_tag="VFX38_07785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX5314784.1"
                     /translation="MTNGIAAGASAERIAPGTLMALVAMGLAVFVLANDFTAMSVALT
                     QIEHDFNSDVTTVQWVINAYALVFGVLIVTGGKLADMFGRRRIFFVGTAIFALFSFLG
                     GIAPNVIWLIGARALMGIGGAMMWPAILGMTFAALPERKAGLAGGMILGIAGLGNAVG
                     PLVGGLLTDAFGWRWIFFLNLPIALIAVVVTWAKVHQPAERAPGQRLDYLGVAVLSLG
                     LIALLVALDQANSWGFSDYRTLILFALCVLLLASFPFVERRAGANALLPAALVRNRQF
                     MAACVSILLLSSTFFAALLYLPEFMQKLLDYSALRAGLGLLPLMASFALVSFVAGALY
                     ERLGAKLVTALGAACMTVGVLFFSMIGPDAGYGSFVGGMILLGVGLGLFLSSATTAGV
                     VALEHSQASLAGGVLYLFQIAGGSVGLGLVTAVFISGALAHVHAADAANQLNGMQEQA
                     VMGVLAGTSTARQLLEQFPALGAHLAEVARAAFAAGMHASFRIVGVLGALGFVVALVF
                     LGGRLHVWRKVGTPGPELRRPSARFHHRAYHWGRAPERSAASAPETEKARSI"
     gene            complement(5406..>5882)
                     /locus_tag="VFX38_07790"
     CDS             complement(5406..>5882)
                     /locus_tag="VFX38_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016643071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314785.1"
                     /translation="LPGLLLVGVGYGLGSIALNTASVLAVPPAHASLAGAMLYMAQLV
                     GGSLGLGAYTLIFSASAVHGLNAAGAAAGFAPDQLAAASRVLLGAGSGNSLLSRFGAS
                     GDALHALARDSFVVGLQTGFLLLLALGALSALTALLFLGNWRLPRATQEIEGLVRE"
BASE COUNT         1125 a         1933 c         1945 g          879 t
ORIGIN      
        1 ctgcccggat tggctccgca cgggctgcgg acgtaaaatc acgacatctt cgttcgtgga
       61 gagagccatg cgtcgtttgt ccgtcgtcgc cgtcttgctg ctgccgctcg ccgcgttcgc
      121 cgcgaacact gcgcccgcaa acgcctccgc agcgacgttt gccatggccg cgcccgcact
      181 cgacgcctac gtcggcagct acgaactcgc gcccggattc gtccttacga tttcccgtcg
      241 gggcgagcag ctctacgccc aggcgaccgg ccagggcgcg gcgccgattt acgaaagcgc
      301 gaaggatcaa ttcttctaca aggtggtgaa tgcgcagatc agcttcgagc gtgacgccgc
      361 gggcaatgtc accgggctcg tgctgcatca gaacggcgat cacccggcac cgcgcatggc
      421 gagcgccgcc tcagcggtga ctgcgatggc gcctgcgagc gcgacgaact ggatcgcggc
      481 ggccgccgtc ggtacgacaa cgctgcccat cacggtgacg cgcgacggct tcgtgctcaa
      541 gggcgtgctc gatcttccgg ccggaaaagg tccgttcggc gtcgttgaca tcattcccgg
      601 cagcggaccg gtgagtctca acggcaacga cggtccgatt acctacagtc cctacaggaa
      661 actcgccgcg gcgctggtcg aggccggctg ggccgtcgcg cgcgtagcca agcgcgggat
      721 ggcgccgtcg acggggaacg gtaacgacgt cgtcttctcc gatcagcttg ccgacaacct
      781 cgcgatcgtc gcggccctgc gcaaaaaccc gcacatcaac ccgcggctca tcgtcgtcgc
      841 cggccacagc ctcggcggtc tgattgcccc gaaactcgcc accgaaacga aactcgccgg
      901 cttgatcctg ctcgaagcac ccggcgaagc catgagcaga atctcgacct cccaggccct
      961 tgcgatgagc cggcaggccg gcgccagcgc cacgaaattg aaggcgatcg ccgcggaaca
     1021 gaaccagttc tacgccgcag tcgccaaggc gccgcccggt cagagtgtac ggttcgccgg
     1081 caaaacgttg cagccggacg cggtgcagtt gttcaagagt tggtatgccc agaagccgct
     1141 cgcgaccgcg cgggaagtca caatcccggt gctcgtcgtg cagggcggtc tggatttcaa
     1201 cgtgccgccc ggcaacggca agcggctcgt aaaggctctg ccgcacgcga cattgctgct
     1261 cctgcccagg atgggccacg cgctcgacga cgccgactgc cgctgtgtcc ggcagctcga
     1321 caccggcaag gatgcgacgc tcgcgcccgg cctcgccgcc ggcatcgtcc gctggctgca
     1381 agcactctag cccttcgccg cagaggcggt gacactccga tcgtcatcag ttcggaggcg
     1441 gcgtctgctc gacgatctcg gttgcgatat ggcagggaat ggcgccgggg tgcatggcga
     1501 tgttggcgat gatcacgacg ccgacgtttt ttttcggcac cccgacaacg caactggaaa
     1561 aatcgtgtgt ttccccatct ttggagaagc gcagatcgta accgggtcgc ggtggggtga
     1621 ccacccaggc gtagccctgg cgcggggtag cgaacgccgc acctgccggc agcggatcga
     1681 ccggcaccgc ggtcaggcga cgtgcaaccg tcatggtcgc ggtaagcggc cacggcacgg
     1741 agcggccggc gacgatgcga ttaccgagat aggcaccaag gtagcgcaac atgtccgcgg
     1801 cactggaacg cagcgatccc gccgggcccc agggaaccca gggccaggga gacgattcga
     1861 tctcgtagtg atcgccgcgc aggtgataac cgcttgccat tcgcgcggtc gcattgcagg
     1921 cctgcggcat cgcgtccgga tccggattgg tgcagcgcat gccgagcggc cgcaggatcg
     1981 cccgtccgat ccactgcgcg aagttcgcgc gaaatgcatc gggatcggaa aagtctgtat
     2041 agccgtcgaa ggacatgaga acctggccga gaataccgta gctcgaattg gaatagaaat
     2101 tcggcgcggg caggccgttc cgcggggccg gccatacatc gaacgccttg atgaaatccg
     2161 cctgggtata gttctcggcg gcggcgaatt tgtagttcgg cggatcgtcg tacaggccgc
     2221 cggtaaaggt ggcgagttcg aaatacgtga gcgcctccat gcctccggtc ggttcgtagc
     2281 agacgctcgt gctctccgca atcacgcacg ggacctcaga gaaatccacg ggatcgtgca
     2341 cgcggatccg cccggcgagt tgatgcaagg ccagcatcgt cgccgtgaag gtcttcgtgt
     2401 tcgaacccat ttcccacagg gtgcgcgccg tcggcgtgct ttgttcgccg agcttcgtcg
     2461 tgccgtagcc gtaatagcag ggctcaccgt cgagccacac gccgacgatc atgcccaccg
     2521 cctgcgtcgc ggtcgcggct ccgccggcat agacgtgcgg ttgcacgatc gggtcgacga
     2581 cgtctttttc gttcgcacac ccgccggacg cgcgcgccgc ggcggtgccc gcaagcgcgg
     2641 cgatcgcgca gacagcaatc agggcgcggc gcatcggcat tcccgctacg ttgccttgcg
     2701 attatagcga agcatcttcg ccgcgccccc gatctccggc ggagttcagg gctcgtcgag
     2761 acggagaacg aggcatttgg cggcgccgcc ggacttcatg aattccgaga cgtccacgcc
     2821 ggtaacttcg aagccggccg tttccagcac gtttcgcagc cgttcgctcg gattgttgag
     2881 gaacacgtgc cggtcgagat tcacggcgtt gcaggacagc gcgtcggcct ccgcggcgtt
     2941 gaccgcgatg cgccgctcgg gcgcaacgcg tcgttcgata gccgcgcggc cctcctcgtc
     3001 gaaggcctca gggtagtaca gaagatagcc gccttcgagc ggggcgaggc aggtatcgat
     3061 gtgatagtag tagggatcga cgagccgcag cgagacggtt tcgcgctcgg gataaaacgc
     3121 cgcgagcctt tcgtgtgccg cgagcgccgt gcgctgtccg tagcccgccc atacccaggg
     3181 accgccgcga tcaagcagcg catcgcccgc gccttcgaag accaactctt ccggccaggc
     3241 gaccagctcg aacccgcgcg atgcgaacca gtcgcgaaaa tgcggttctt cgccgcgacg
     3301 ttcgggcggc gagaaacggc tgacgatgac gcgccggccg ttcacgaccc cggcgttcgc
     3361 cgtgaagacc atgtccggca gttgcggctg cgccggaatc aattcgactt cggcgacgcg
     3421 cgcgagcaca tcacgcagcg cctgccattg cgcgagcgcg cggccgcgcg aaacttcgcc
     3481 ctcttcgccg tccatccagg gattgatgtt gtaactgacg gtgaagtgat cgggccggca
     3541 catgagaatc ttgtggcgcg cgggcatgac gttgctccgg ggaagacggc gatcggccac
     3601 gccgatcgtt gcatattgta aggaatattc ggcccttaga tcgaacgcgc tttttccgtc
     3661 tcgggcgcgg aagccgccga gcgctccggc gcccggcccc aatggtaggc gcggtggtga
     3721 aaacgcgcgg agggacggcg caattccgga cccggcgtgc cgactttgcg ccacacatgc
     3781 aggcgtccgc cgaggaaaac cagcgccacc acgaaaccca gcgctccgag gaccccgacg
     3841 atgcggaagc tcgcatgcat gccggccgcg aaggccgcgc gcgcgacctc ggcgagatgc
     3901 gcgccgagcg cggggaactg ctcgagcagt tggcgcgccg tgctcgttcc cgccagcact
     3961 cccatcacgg cctgctcctg catgccgttc agttggttgg cggcatccgc cgcatgcacg
     4021 tgagccagcg cccccgaaat gaaaaccgcg gtcacgagtc cgaggcctac cgagcctccg
     4081 gcaatctgga aaagatagag gacgccaccc gcgaggctcg cctgcgaatg ctccagcgcg
     4141 acgacccccg ccgtcgtcgc cgaagaaagg aacagcccga gtccgacgcc gagaaggatc
     4201 atgccgccga cgaaggatcc gtaccccgca tcggggccga tcatggaaaa gaacaagaca
     4261 ccgaccgtca tgcaggccgc gccgagcgcg gtgacgagct tcgcgccgag gcgttcgtag
     4321 agtgcccctg cgacgaagga aacgagggcg aacgacgcca tgagcggcag gagaccgagc
     4381 ccggcgcgca aggcggaata gtcgagcagc ttctgcatga actccggcag atacagaagg
     4441 gccgcgaaaa acgtgctcga aaggagcagg atcgaaacgc aggccgccat gaactggcgg
     4501 ttgcggacaa gcgcggcggg gagcagcgca ttcgctcccg cacgccgctc gacgaaggga
     4561 aacgaggcaa gcagcagcac gcagagcgca aacagaatga gcgtgcggta atcggaaaat
     4621 ccccaggaat tcgcttgatc cagcgcgacg agaagcgcga tgagcccgag cgaaagcacc
     4681 gcgacaccga gataatcgag acgctgcccc ggcgcgcgtt ccgccggctg atgcaccttc
     4741 gcccaggtga cgacgacggc gatgagggcg atcggcagat tgagaaagaa gatccagcgc
     4801 cagccgaagg cgtcggtgag taggccgcca accagcgggc cgacggcgtt gccgagcccg
     4861 gcgatgccga gaatcatgcc gccggcgagt cccgccttgc gttcgggaag cgcggcgaag
     4921 gtcatgccga ggatcgccgg ccacatcatc gcgccgccga tgcccatgag cgcgcgcgcg
     4981 ccgatgagcc agatcacgtt cggcgcgatg ccgccgagaa aggagaagag tgcgaagatc
     5041 gccgttccga cgaagaagat gcgccgccgg ccgaacatgt cggcgagctt gccgccggtc
     5101 acgatcagca cgccgaagac gagcgcgtag gcgttgatca cccactgcac cgtggtcacg
     5161 tcggagttga aatcgtgctc gatctgggtg agcgcgacgc tcatcgcggt gaaatcgttc
     5221 gcgagcacga agaccgcaag ccccatcgcg accagcgcca tgagcgttcc cggagcgatt
     5281 ctctccgccg atgcaccggc ggcgatgccg ttcgtcacgc tttcatcccc gggcgtccgt
     5341 ggcttcgcat catactcgat gaaaatttcg aggcaggcgt ctccctgcga ctactccgtg
     5401 cggaatcatt cgcgaacgag gccctcgatt tcctgcgttg cgcgcggcag gcgccagttc
     5461 cccaggaaga gtagcgcagt cagcgcggaa agcgcgccga gcgcaagaag cagcaggaat
     5521 ccggtctgaa gcccgacgac gaaggaatcg cgcgcgagcg cgtggagcgc atcgcccgaa
     5581 gcgccaaagc gggaaagaag cgaattcccc gagcccgctc cgagaagaac ccgggaggcg
     5641 gctgcgagtt gatcgggcgc gaagcccgcc gcggcgcccg cggcattcag gccgtgaacg
     5701 gcgctcgcgc tgaaaatcag cgtgtaggct ccgagcccga gggagccgcc gacgagctgc
     5761 gccatgtaga gcatcgcccc ggcgagactc gcatgcgccg gcggtaccgc gagcacgctc
     5821 gcggtgttga gcgcgatcga accgagaccg tagccgacgc cgacgagaag gaggcccgga
     5881 ag
//
LOCUS       DASUJG010000364         4664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1084143, whole genome shotgun sequence.
ACCESSION   DASUJG010000364 DASUJG010000000
VERSION     DASUJG010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4664
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084143"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /gene="gidA"
                     /locus_tag="VFX38_07795"
     CDS             <1..451
                     /gene="gidA"
                     /locus_tag="VFX38_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009150274.1"
                     /note="GidA; glucose-inhibited cell division protein A;
                     involved in the 5-carboxymethylaminomethyl modification
                     (mnm(5)s(2)U) of the wobble uridine base in some tRNAs;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX5314786.1"
                     /translation="EARRFTPRELSAAWIAEHLGEAPADSATAWQLLARPKMSYAAIA
                     ELAPAAAPIAADVAALLEAEAHYGGYLKRQSAEVLRLRARETLALPGDFDYAKVRGLS
                     NEVREKLAAARPATLGQAARIPGVTPAAVSLLLVHLSAAERARVARG"
     gene            555..2132
                     /locus_tag="VFX38_07800"
     CDS             555..2132
                     /locus_tag="VFX38_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HEX5314787.1"
                     /translation="MKRNFLLATAFSLSLVALPAFAGAPAPPPPPASAPSAQQSITHG
                     SVTVGGKSIDYTAIAGIMVLKNDDGKPYASMSYVAYVKSGGDANRPITFFYNGGPGSS
                     TVWLHMLAFGPKRVVVGNGTLTPSAPYQLVNNDESLLDATDEVFVDAPGTGFGRVIGK
                     DRGGVGKPEDVFGLDPDAEAFTRFITGYLTAHNRWNSPKFLFGESYGTTRSAVLAGDL
                     QQANIDVNGIVLLSSILNFDISVDGAEGNPGVNISYATGLPSYAATAWYHKKLPQQPK
                     DLKSFLAQVEHFAMNDYLLALNEGNRITPAAKKRIADQMHRYTGLPVAYILKADLKVT
                     GGQFAHELLGATDEITGRLDSRYSGPAMDPLAESAGYDPLDSAIDSATASLFNSYVRN
                     TLKFGQNLQYRPTAYAQIYGKLGGWSWKHKAPGSNYAGSGTPNVMPDLAAAMKFNPNM
                     KILLAGGYFDLGTPFYAAEFEMHQLPIQPSLQQNISYHFFPSGHMVYVNPTAHKGLHD
                     AAAAFIEANYQHPKQNQ"
     gene            complement(2140..2700)
                     /locus_tag="VFX38_07805"
     CDS             complement(2140..2700)
                     /locus_tag="VFX38_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX5314788.1"
                     /translation="MANDEITALDGGCDCGFVRYRMTGAPLFVHCCHCRWCQRETGSA
                     FVLNAMIETDRVHLLAGEPEIVNTPSASGKGQKIARCPSCRIALWSHYAGGGDAVRFV
                     RVGTLDEPDRLPPDVHIFTASKQPWVIIPPDMPAFAAFYEPEKCWPAASLERRRALLQ
                     TRTQAGRNPAKRGVAGNHPLRPGTRR"
     gene            complement(2736..3446)
                     /locus_tag="VFX38_07810"
     CDS             complement(2736..3446)
                     /locus_tag="VFX38_07810"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX5314789.1"
                     /translation="MPGSLGHVTAVSPRPWVVLLRAIGLMLVTFLAVYVPASLLTKGL
                     RLPINAAVPVEILVTFTIACAIAGWHIRRDGFTAAAFGARLPPRRYVGYALLFAAPLS
                     VLVAWFLGHFAESGPLAGLSLAPWLVYLYFVVAAPIQEEMIFRGLLQTALARRLALQR
                     KYAAASGVIAVLAVAILFAVIHLVVGAATAAGALVLGLLAGELRRRSGSLLPAIVCHS
                     IFNLAGILWTLHLHQAGG"
     gene            complement(3457..>4664)
                     /locus_tag="VFX38_07815"
     CDS             complement(3457..>4664)
                     /locus_tag="VFX38_07815"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX5314790.1"
                     /translation="FLADLDGWPHLYSIAKAGGEPLLLTPGDYMVEDVTESPDHQFLV
                     YNANTGATPGDGDRRHLYRVPVGAAQPVALTSGATIEWSPEITSDGKDVAFIRAGAQL
                     PPLIALADVDGSGARDLDANQLPADFPTAQLVVPRPVTFKAADGWTIQGQLFQREDGN
                     AAKPGIIFVHGGPPRQMLLGWHYMGYYSNSYAVNQYLANHGFVVLSVNYRLGIGYGHA
                     FHNPEHWGPTGASEYQDVVAGAKYLQRLRGVDPKRIGIWGGSYGGYLTALALARNSNI
                     FKAGVDMHGVHDWSRFIDDWFGKLQPRYEQGDRKKAMKVAWESSPDSAIAGWKSPVLL
                     IQGDDDRNVHFHQMVDLARRLHKQRVPFQEIVIPNEIHGFLRYRSWLLADTATVQFFE
                     AQFGRSNGK"
BASE COUNT          858 a         1504 c         1503 g          799 t
ORIGIN      
        1 cgaagcgcga cgcttcacgc cgcgcgagct ttcggctgcc tggatcgcgg agcatctcgg
       61 cgaggcgccg gcggattccg cgacggcctg gcagttgctc gcacgtccga agatgagtta
      121 cgcggcgatc gccgaactcg cgcccgccgc tgccccgatc gcggcggatg tcgcggcgct
      181 gctggaggcc gaggcgcatt acggcggcta tctcaagcgc cagagtgcgg aggtcctgcg
      241 gctgcgggcg cgggagacgc ttgcgcttcc cggcgacttc gattacgcga aggtgcgcgg
      301 gctttccaac gaggttcgcg agaaactcgc ggcggcgcgc ccggcaacgc tcgggcaggc
      361 cgcacgcatt cccggcgtga ctccggcggc ggtctcgctt ctcctcgtgc atctcagcgc
      421 cgcagagcgc gcccgcgtcg cccgcggctg agcgcgaggc gctatactgc ggcgcagccg
      481 ccgggacgat tcggcggcct tcgcatcacg tccgccggtc gcggttcttc cggcggagaa
      541 aacaggagat tccgatgaag cgcaactttc ttcttgcgac ggcattttcc ttgagcctcg
      601 tcgcgctgcc cgcgttcgcg ggagcacccg cgccgccgcc gccgcccgcg agcgcgccgt
      661 ccgcccagca gtccatcacg cacggttcgg tgacggtggg cggcaagagc atcgactaca
      721 cggcgatcgc cgggatcatg gtgctcaaga acgacgacgg caagccctat gcaagcatgt
      781 cctacgtcgc ctacgtcaag agcggcggcg acgcgaaccg tccgatcacc ttcttctaca
      841 acggcggacc gggctcctcg accgtgtggc tgcacatgct cgccttcgga ccgaagcgag
      901 tggtggtcgg aaacggcacg ctcacgccgt ccgcgcctta tcaactcgtc aacaacgacg
      961 aaagcctgct cgatgcgacc gacgaggtgt tcgtggacgc gcccggcacc ggcttcggcc
     1021 gcgtgatcgg caaggatcgg ggcggagtcg gaaagcccga ggacgtcttc ggtctcgatc
     1081 ccgatgccga ggccttcacg cgcttcatca ccgggtacct cacggcgcac aaccgctgga
     1141 actcgcccaa gttcctcttc ggcgaaagtt acggcacgac ccgctcggcc gtgctcgcgg
     1201 gcgatctcca gcaggcgaac atcgatgtga acggcatcgt gctcctgtcc tcgattctca
     1261 acttcgacat cagcgtggac ggagccgagg gcaatcccgg cgtcaacatt tcctatgcga
     1321 ccgggcttcc gagctatgcg gcgaccgcct ggtatcacaa gaagctgccg cagcagccga
     1381 aggacctgaa gagctttctc gcgcaggtcg agcacttcgc gatgaacgac tatctgctcg
     1441 cgctcaacga gggcaatcgc atcacgccgg cggcgaagaa aaggatcgcc gatcagatgc
     1501 atcgctacac ggggcttccc gtcgcctaca tcctgaaggc cgatctcaag gtcaccggcg
     1561 gtcagttcgc gcatgaactt ctgggcgcga cggacgagat caccgggcgg ctcgattcgc
     1621 gctacagcgg ccccgcgatg gacccgctcg ccgagtcggc gggctacgat ccgctcgatt
     1681 ccgcgatcga ttccgcgacc gcctcgctgt tcaactccta cgtgcgcaac acgctcaagt
     1741 tcggccagaa cctgcaatat cgtccgacgg cctacgccca gatctacgga aaactcggag
     1801 gctggagctg gaagcacaag gcgccgggtt ccaattacgc gggctcgggc acgccgaacg
     1861 tgatgccgga tctcgcggcg gcgatgaagt tcaatccgaa catgaagatt ctgctcgccg
     1921 gcggctattt cgatctcggc actccctttt atgcggccga gttcgagatg catcaattgc
     1981 cgatccagcc cagcctgcag cagaacatca gctatcactt cttcccgtcg ggccacatgg
     2041 tgtacgtcaa tccgacggcg cacaagggtc tgcacgacgc cgcggcggcc ttcatcgagg
     2101 cgaattacca gcatccaaag cagaatcagt aggccgggat cagcggcgcg ttcccggccg
     2161 caacggatga ttgccggcaa caccccgctt tgcggggttt ctgccggcct gggttcgtgt
     2221 ttggagtaac gcgcggcgcc gttcgagact tgccgccggc cagcattttt ccggctcgta
     2281 gaatgccgcg aacgcaggca tgtccggcgg aatgatgacc cagggctgtt tcgaggcggt
     2341 aaaaatgtgc acgtccggcg gcaggcgatc cggttcgtcg agcgtgccga cccgcacgaa
     2401 tcgcacggcg tcgccgccgc cggcgtagtg gctccacagc gcgatccgac agctcggaca
     2461 gcgcgcgatt ttctgacctt tgccgctcgc cgatggggtg ttcacgattt ccggctcacc
     2521 ggccagcaaa tggacgcgat cggtttcgat catggcattg agcacgaagg ccgatcccgt
     2581 ctcgcgttga caccagcggc aatggcagca gtgcacgaag agcggcgcgc ccgtcatgcg
     2641 atagcgcacg aagccgcaat cgcagccgcc gtcgagtgca gtaatttcgt cgttggccat
     2701 cgggcaggtc tcctctgtca gaatcgttct gtggactagc cgccggcctg gtggaggtgc
     2761 agggtccaca ggatgccggc aaggttgaag atggaatgac agacgatggc gggcagcagg
     2821 ctcccgctgc gccttctcaa ctccccggcg agcaatccga gaacgagcgc gccagctgcc
     2881 gttgccgctc ccaccaccag atgaatcacg gcgaaaagta tggcgacggc gaggactgcg
     2941 atcactccgg aagcggcggc gtattttcgc tggagcgcga gccgtcttgc caacgcggtt
     3001 tgcaacaagc cccggaaaat catttcttcc tggatgggtg ccgccaccac aaaatacagg
     3061 tagaccagcc agggagccag gctcaatccg gccagcggtc cggattctgc gaagtggccg
     3121 agaaaccacg cgacgagcac gctcagtggc gccgcgaaaa gcagcgcgta gccgacgtag
     3181 cgccggggcg gcagcctggc gccgaacgcg gccgccgtga atccatcacg acggatgtgc
     3241 cagcctgcaa tggcgcaggc aatcgtgaaa gtgacgagga tctcgaccgg tactgcggcg
     3301 ttgatcggca agcgcaggcc tttcgtcaac aaggaagccg gaacgtacac ggcaagaaac
     3361 gtgaccagca tgaggccgat cgcccgtagc aggacgaccc agggccttgg ggaaacggca
     3421 gtcacgtggc cgagtgatcc gggcatacgg attcggctac ttcccgttgc ttcgcccgaa
     3481 ctgcgcttcg aagaattgca cggtggcggt gtcggcaagc aaccaggagc gatagcgcag
     3541 gaagccgtgg atttcgttcg gaatgacgat ttcctgaaac ggaacccgct gcttgtgcag
     3601 ccgccgtgca aggtccacca tctgatgaaa atgcacgttg cggtcgtcgt cgccctgaat
     3661 gagcagcacc ggcgacttcc agccggcgat cgcggaatcc ggcgaggatt cccaggcgac
     3721 cttcatcgcc ttcttgcggt cgccctgctc gtagcgcggc tggagttttc cgaaccagtc
     3781 gtcgatgaag cgtgaccagt cgtgcacgcc gtgcatgtcc acgccggcct tgaagatgtt
     3841 ggagttgcgc gcgagcgcga gcgcggtgag atagccgccg taggagccgc cccagatgcc
     3901 gatgcgcttc ggatcgacac cgcgcagcct ttgcagatac ttcgcgcccg cgaccacgtc
     3961 ctgatattcg ctcgcgcccg tcggtcccca atgctcggga ttgtggaagg cgtggccgta
     4021 gccgatgccg aggcggtaat tgaccgagag cacgacgaag ccgtgattgg cgagatactg
     4081 gttgaccgcg taggaattgc tgtaatagcc catgtaatgc cagccgagca gcatctggcg
     4141 cggcggtccg ccgtgcacga agatgatgcc aggttttgcc gcattgccgt cttcgcgttg
     4201 aaagagctgg ccctgaatcg tccagccgtc ggcggccttg aaggttaccg gtcgcggaac
     4261 gacgagctgc gcggtcggga agtcggcggg cagttgattc gcatcgagat cacgggcgcc
     4321 gctgccgtcc acatcggcga gcgcgattag cggcggtagt tgcgcgccgg ctcggatgaa
     4381 cgcaacgtcc ttgccgtcgc tcgtgatttc cggactccat tcgatcgtcg cgccgctcgt
     4441 gagcgcgacg ggttgcgccg cacccacggg cacgcgatac aagtgacggc gatcgccgtc
     4501 tcccggcgtc gcgccggtgt tcgcgttata aacgagaaac tggtggtcgg ggctttccgt
     4561 cacgtcttcg accatgtagt cgcccggcgt gagcagcagc ggctcgccgc ccgccttcgc
     4621 gatcgaatac agatgcggcc agccgtcgag atcggcgaga aaca
//
LOCUS       DASUJG010000365         5268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_716057, whole genome shotgun sequence.
ACCESSION   DASUJG010000365 DASUJG010000000
VERSION     DASUJG010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5268
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716057"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VFX38_07820"
     CDS             <1..402
                     /locus_tag="VFX38_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314791.1"
                     /translation="APRGGGDVEPPRLNDGAQTTTMLPSRSEQAGMTQHDDPASPRPE
                     LDDSAAITEQDEEYASRSIPPVTRRVRYWHIYRGAGIVTACAGVVMMIAGHFADSHRS
                     MQIAGFLILSGAVVFTIGVIGSWITRQRPLD"
     gene            complement(464..934)
                     /locus_tag="VFX38_07825"
     CDS             complement(464..934)
                     /locus_tag="VFX38_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RT0821/Lpp0805 family surface protein"
                     /protein_id="HEX5314792.1"
                     /translation="MHRRVVLTLGLALFAATLVLAGCASKEQTGAVLGAGAGAAIGHQ
                     FGGGRGQTLMTIAGAIGGALAGSAIGKKMDDNDRQKTASALENTPTGQTSTWTNPDTG
                     QTYAVTPTNTTTENSEPCRTFVFKSYNSTTGQPQSVTQLACRQPNGTWQIQSGQ"
     gene            1406..>5268
                     /locus_tag="VFX38_07830"
     CDS             1406..>5268
                     /locus_tag="VFX38_07830"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HEX5314793.1"
                     /translation="MALRRRVEVLIFASLMVAATAASAAGGDLHLRRISPSGDDVPTG
                     QEIVFQFDRAMVALGNMARSPADIPITIEPAVGCEWRWLDTSELVCRLPGKATLRPAT
                     RYTITVGSDFRALDGARLAHAVTSGFTTILPQVDWLSFQQWRSPVMPVYLLRFNLPVT
                     AEAVAAHVGFNGNDGHNVAALADPFTKKREGPVWLSVEGMPGLLLEIENPQPATPLDA
                     RNAAGEARLQWLVRPAKPLEAASEYDLALAPGLTTPLGELSAKGGLPTRAHLETWGPF
                     RFRGVACEDVHANSVDVAPGESQRVPCEPTSLRLAFSAPVPLSTLAVAEWKPAPRPLP
                     EMKRKWLGYPVWYLGIPQDPTGAARNQSFPLPFDMKATSAYRITIPAGVHDAFGRTLA
                     EATTVTVRTGHFAPFFDGPPDEAVLEAKVPTIVPLRFVNLKRLEFRYRRLLAQDLGHP
                     VAPLRRPPAAATSILARSGLRVREDRIVTVPLGLRKLLGGHAGAAWGEISWAPGPGTT
                     RSASQNFFGEVTPWQVFAKIGYFDSLVWVSSLATGAAIPQAKVAFYVGHPESLDRLER
                     LGGKPDKTDLGGLAVLPGTLGFGADWFQPWENGALHYYVGVTENGRLALLPLDWSFRR
                     YIGDASDYNLYENNAPAWGHLEAWAMTQQGIYRPASDVHFALFVRNVSNASLVAPPAL
                     DYGLKIEDPTGTAVLEQKHLKLGPFGGFATTLHLPKTAPMGWYAIQLSWPTSTGKESR
                     EIGRFLVTEFVPASFKVSAMLAGSQFAPGESVRASARARLHAGGPYSDADIKFSAEID
                     AEAFQPDTPEAAGFSFAANPASAPSSLAVGSIETRLDHQGEAATLFRLPENPPIYYGR
                     LALEAAVKSERGSWVANRTSAQFAARDRFVGLHLEDWLLTAQSPATVQYLVVNAKGKP
                     AAGAPVHLLLQRQVVTSARVKNAAGYFTANQQTMWKDEDSCRAVSAAKPGSCTLTPRH
                     AGSYRIVAEVRDSEGREQTSTLQTWATGPGAVVWPQNKRVGLVPDKSEYHPGDTAHVL
                     VQNPFPGAVALVTVERYGVLWKKTMPLAGSTPLLDIPIEPGFFPGAYLSVAVFSPRVD
                     HPGSPDLGRPELALGYVALPVSGNGSSLAVKVTPAERVYKPGETVAVKVHVAREDGKT
                     SAGTRLVAVVLDQGVLDLLEQGKDYYDPRKRFYAPPEGPDMLNFSLAEQLLTELQPKT
                     GKGKSPGGGGGAGPDIRTIFKYSAYWNPTLATDAAGNANFSFTLPDNLTRWRIFVIAM
                     TPAAAMGLGDASVRVNLPIQIQPALP"
BASE COUNT          877 a         1809 c         1669 g          913 t
ORIGIN      
        1 gccccgcgcg gggggggcga cgtcgagccg ccacgcttga acgacggggc gcaaactact
       61 accatgcttc cttcgcgctc cgaacaggcc ggcatgaccc agcacgacga cccggcgagc
      121 cctcgtccgg aactggacga cagcgccgcc atcaccgaac aggacgagga atacgcctcg
      181 cgcagcattc cgccggtcac tcggcgcgtg cgttactggc atatttatcg cggcgcgggc
      241 atcgtcaccg cctgcgccgg ggtggtgatg atgatagccg gccactttgc cgattcgcat
      301 cgcagcatgc agatcgcggg atttctgatt ctctcgggtg ccgtcgtctt cacgatcggc
      361 gtaatcggca gctggatcac ccggcaacgt ccgcttgatt gaggcgccgg gcaggagcgg
      421 tgcgagcgca ccgttcctgc ctcgcccgat cacgcggcct tagttactgc ccgctctgga
      481 tctgccaggt cccgttgggc tgccgacagg cgagctgtgt caccgactgc ggttggcccg
      541 tcgtcgagtt gtagctcttg aacacgaagg tccggcaggg ttcgctgttc tcggtcgtag
      601 tgttcgtcgg cgtcaccgca taggtctggc ccgtatccgg attcgtccag gtcgaggtct
      661 ggccggtcgg cgtattttcc aatgccgaag ccgttttttg ccgatcgttg tcgtccatct
      721 tcttgccgat cgcactgccc gcgagcgcac cgccgatggc gccggcgatg gtcatcaggg
      781 tctgcccgcg tccgccaccg aactggtggc cgatcgccgc tccggcgccg gcgccgagaa
      841 cggctccggt ctgctccttg ctcgcgcaac ccgcaagaac gagcgttgcc gcaaacagcg
      901 cgaggccaag ggtaagtact acgcgtctgt gcatcttgca ctccatgttg atccacgttc
      961 ttgcagtgta gcgcaaagcc ggcgccggcc gcagggccgc aagcgcgcat caccgcaatt
     1021 gaagtgcctt catggaattg gacgcaataa attgattatc cttatgatcc gcctcagaga
     1081 agccgtgaag tgacggcgtc cgccagctca cggacacgga acctttgccc ccttcggagc
     1141 gaagggggcc gcgtcttcgc gggggatgtc cggcgtcggc gcacgaaaga catctctctc
     1201 gatcccactt cctgcggaag ggggaagccg tactcccaac ttccgcgacg ttgtcgtggt
     1261 tgcgacttcg cggagtccct tcgccccctt cggaacgaag agggccgcgt cttcgcgggg
     1321 agatgtcttt gtttctcgcg atcggaacaa accaaacgct cggctatact gcggtgcatc
     1381 ggcagctgac gcggcggcac gaacgatggc attgcggcgc cgggtcgaag tcctgatctt
     1441 cgcgagcctg atggtggcag caacggctgc atccgccgcg ggcggcgacc ttcacctccg
     1501 tcgcatctcg ccgagcggcg acgacgtgcc gacggggcag gaaatcgtgt tccagttcga
     1561 ccgcgcgatg gtcgcgctcg gcaacatggc gcgcagtccc gccgacattc cgatcacgat
     1621 cgagcccgcc gtcggctgcg aatggcgctg gctcgacacc agcgaactcg tctgccgtct
     1681 gccgggcaag gcaaccttgc gtccggcgac gcgctacacg atcaccgtcg gcagtgattt
     1741 tcgcgcgctt gacggcgcgc ggctcgcgca tgcggtcacg agcgggttca cgaccatcct
     1801 tccccaggtg gactggttga gcttccagca gtggcgctcg ccggtaatgc ccgtttacct
     1861 gctgcgcttc aatctgccgg taacggccga ggcggtcgcc gcgcacgtcg gtttcaacgg
     1921 aaatgacggg cacaacgttg ccgcgctggc ggatccgttc acgaaaaaac gcgaaggacc
     1981 ggtgtggctt tccgtcgagg gcatgccggg tctcctgctc gaaatcgaga atccgcagcc
     2041 cgcgaccccg ctcgatgctc gcaatgccgc gggcgaagca cggcttcaat ggctcgttcg
     2101 tccggccaag ccgctggagg cggcgagcga gtatgacctc gcgctcgcgc ccggattgac
     2161 gacgccgctc ggagaattga gcgccaaggg agggcttccg acccgggcgc atctcgaaac
     2221 ctggggcccg ttccgcttcc gtggtgtcgc ctgcgaggac gttcatgcga attcggtcga
     2281 cgtcgcgccg ggcgagtccc agcgcgttcc ctgcgagccg acgagcctgc gccttgcctt
     2341 cagcgcgccc gtgccgctgt ccacccttgc ggtcgccgaa tggaaaccgg cgccgcggcc
     2401 gctgccggaa atgaaacgca aatggctggg atatcccgtc tggtatctcg gcatcccgca
     2461 ggacccgacg ggcgccgccc gcaaccagtc ctttcctttg cccttcgaca tgaaggcaac
     2521 gagcgcgtac cggatcacga ttcctgccgg ggtgcacgat gccttcggcc gaacgctcgc
     2581 cgaagccacg accgtaaccg tgcgcaccgg ccacttcgcg ccatttttcg acggcccgcc
     2641 ggacgaggcg gtgctggaag ccaaggttcc gaccatcgtg ccgctgcgct tcgtcaacct
     2701 caagcgcctc gaattccgct accgccggct gctcgcgcag gacctcggcc acccggtcgc
     2761 accgctccgc cgcccgcccg cagccgccac ctcgattctc gcgagatccg ggctccgcgt
     2821 gcgcgaggac cggatcgtga ccgtgcccct gggcctgcgc aaacttctcg gcggacatgc
     2881 gggtgccgcc tggggcgaga tttcctgggc tcccggcccc ggcaccacgc gcagcgcgag
     2941 ccagaacttc ttcggcgagg tcacgccctg gcaggtgttc gccaagatcg ggtatttcga
     3001 cagcctcgtg tgggtgagct cgctcgcgac cggggccgcg attccgcaag ccaaggtcgc
     3061 gttttacgtc ggtcatcccg aaagcctcga tcggctcgaa cggctcggcg gcaagcccga
     3121 caagaccgat ctcgggggac tcgccgttct cccgggaacg ctcggcttcg gcgccgactg
     3181 gtttcagccc tgggaaaacg gcgcgcttca ctactacgtc ggggtcaccg aaaacggcag
     3241 gctcgcgctg cttccgctcg actggtcgtt ccgccggtac atcggcgatg cgagcgacta
     3301 caacctctac gaaaacaacg ctcccgcctg gggacacctc gaggcctggg cgatgacgca
     3361 gcagggtatc taccggcccg cgagcgacgt acacttcgcg ctcttcgtgc gcaacgtgag
     3421 caacgcgagt ctcgtcgccc cgccggcgct cgactacggc ctgaagatcg aagatccgac
     3481 cggcaccgcg gtgctcgagc agaagcacct caagctcggg cctttcggcg gcttcgcgac
     3541 cacgctccac ctgcccaaga ccgcgccgat gggctggtac gcgatacaac tttcctggcc
     3601 gacctcgacg ggaaaggagt cgcgggaaat cggccggttc ctcgtcaccg agttcgtgcc
     3661 cgcgagcttc aaggtttcgg cgatgctcgc cggttcgcag ttcgcgcccg gcgaaagcgt
     3721 gcgcgcgagt gcccgggcac gtctgcacgc cggcggcccc tacagcgacg ccgacatcaa
     3781 gttctccgcc gagatcgacg ccgaagcctt ccagcccgac acacccgaag ccgcgggctt
     3841 cagcttcgcc gcgaatcccg cgagcgcgcc ctcttcgctc gccgtcggca gcatcgagac
     3901 caggctcgat catcagggcg aggcggcgac gctgtttcgt cttccggaaa acccgccgat
     3961 ttattacgga cggctcgccc tcgaagcggc ggtgaaatcc gagcgcggga gctgggtcgc
     4021 gaaccgcacg agcgcgcagt tcgccgcccg cgaccgcttc gtcggcctgc atctcgagga
     4081 ctggctgctc acggcgcaaa gcccggccac ggtgcaatac ctcgtcgtga acgcgaaggg
     4141 aaaacccgcc gcgggcgctc ccgtgcatct tctgctgcaa cggcaggtcg tgacgagtgc
     4201 gcgcgtcaag aatgccgccg gctacttcac ggcgaatcag cagaccatgt ggaaggacga
     4261 agattcctgc cgcgcggttt cggctgcgaa gccgggaagt tgcacgctga ctccgcggca
     4321 cgcgggcagc tatcgcatcg ttgcggaggt ccgcgattcc gaaggtcgcg agcagacgag
     4381 cacgctccag acctgggcaa ccggccccgg cgcggtcgtc tggccgcaga acaaacgcgt
     4441 cggcctcgtt cccgacaaaa gcgaatacca tcctggcgac accgcgcatg tgctggttca
     4501 gaatccgttt ccgggcgcgg tcgcgctggt gacggtcgaa cgctatggcg tgctctggaa
     4561 gaagacgatg ccgcttgccg gcagcacgcc gctgctcgac atcccgatcg agccaggctt
     4621 ctttcccggc gcctatctct cggtcgccgt tttttctccg cgggtggatc atcccggatc
     4681 gcccgatctc ggccgtcccg aactcgcgct cggctatgtt gcacttcccg tatcgggcaa
     4741 cggcagttcg ctcgccgtaa aggtgacgcc cgcggagcgc gtttacaagc cgggagaaac
     4801 ggtcgcggtc aaggtgcacg tcgcgcgtga agacggcaag acgtcggccg ggacgcggct
     4861 tgtcgcggtc gtgctcgatc agggcgtact cgatcttctc gagcaaggca aggattacta
     4921 cgatccgcgc aagcgcttct acgcgccgcc cgaaggcccg gacatgctca acttcagtct
     4981 cgccgagcag ctcctgaccg aactgcaacc gaagaccggc aaggggaaga gccccggcgg
     5041 cggaggcggt gccgggccgg atatccgcac cattttcaaa tacagcgcct actggaatcc
     5101 gacgctcgca accgatgcgg ccggcaacgc gaatttttcc ttcacgcttc ccgacaatct
     5161 cacgcgctgg cgcatcttcg tgatcgcgat gacgcccgcg gccgcgatgg gactcggcga
     5221 tgccagcgtc cgagtgaatc taccgatcca gattcagccg gcgctgcc
//
LOCUS       DASUJG010000366         3304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_594939, whole genome shotgun sequence.
ACCESSION   DASUJG010000366 DASUJG010000000
VERSION     DASUJG010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594939"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1538)
                     /locus_tag="VFX38_07835"
     CDS             complement(57..1538)
                     /locus_tag="VFX38_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5314794.1"
                     /translation="MSAIGSAKAAAPPGAITESINPSTGDPIARVRTAARADYDRVVA
                     RASEAFRAWRLVPAPERGEAVRRLGVALREHKSDLARLVMLEVGKISSEAAGEVQEMI
                     DMCDYAAGLSRQLYGLTIASERRQHRLFEQWHPLGPVGVVTAFNFPVAVWAWNATLAA
                     VCGDTVIWKPSPQAPLTAVAVQRIAERVLAECGAPAVWNLCVAGSEAGRWMAEDRRLP
                     LVSATGSCAMGRALAPAVAARLGRSLLELGGNNGVIVTQSADLDLALEAVVFAAAGTA
                     GQRCTTLRRLIVHESVAEPFATRLVDAYRRLRIGPPWKDGVLVGPLINAEARAAMLHA
                     LEAAVEQGGEILCGGRALDGPGYFVEPAIVRAPHDLPIVATETFAPILYVMTYRDLDE
                     ALALHNSVEQGLSSAIFTERLRDAERFLSVEGSDCGIANVNVGTSGAEIGGAFGGEKD
                     TGGGREAGSDAWKSYMRRQTCTVNYGTRLPLAQGVRFDIAPRS"
     gene            complement(1839..3158)
                     /locus_tag="VFX38_07840"
     CDS             complement(1839..3158)
                     /locus_tag="VFX38_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HEX5314795.1"
                     /translation="MNSENTQSSVPCLVTPLPGPRARALIESDERYTSPSYTRVYPLA
                     VERAQGAVVEDVDGNRFLDFTAGIAVCSTGHCHPQVVEAIQRQAAKLLHMSGTDFYYA
                     PQRDLAKKLAEIAPGDVPKRVFFTNSGAEAIEAAFKLARFHTGRKHMIAFFGAFHGRT
                     MGALALTGSKATQRAGFAPLIPEVTHVEFPNPLRGPTVDECMQQIERDVFGRTVPPEE
                     VAAFVVEPIQGEGGYIVPPPDFHPRLKALAERHGICYVVDEVQSGMGRTGRMFAIEHT
                     GVVPDIVCLAKGIASGMPLGAIIARADIMDWPPGSHGSTFGGNPVSCVAALATIDLLE
                     RGLIENARRTGDYLRARLTTLAAERDTIAEVRGVGLMLGVEIVSDRRSMRPAPDERNR
                     IVRACFEQGLLLLGCGESTVRFCPPLIVTERDADTAVDIFEAALAAG"
BASE COUNT          498 a         1167 c         1133 g          506 t
ORIGIN      
        1 gcgcggccgg gtatcgaatg cccgtcgccg aggcggctgc cgccgatctc gccgcctcac
       61 gagcgcggag cgatgtcgaa gcgcactcct tgagccagcg gcaatcgcgt gccgtagttc
      121 accgtacacg tctgccgccg catgtacgat ttccaagcgt cgctgccggc ctcgcggccg
      181 ccgcccgtgt ccttctcgcc tccgaaggct ccgccgatct cggcgccgga ggtgcccacg
      241 ttgacgttgg cgatgccgca gtcgctgcct tcaaccgaaa ggaaacgctc ggcgtcgcgc
      301 agcctctcgg taaagatcgc ggacgacaag ccctgctcca cggagttgtg cagcgccagc
      361 gcttcgtcga gatcgcgata cgtcatcaca tagagaatcg gcgcgaaagt ttccgtcgcg
      421 acgatgggca agtcgtgagg ggcgcgaacg atggccggct cgacgaaata gccgggcccg
      481 tcgagcgctc ggccgccgca aaggatctcg ccgccttgct cgacggcagc ctcgagcgcg
      541 tggagcatcg ccgcgcgtgc ctcggcgttg atcaagggac cgaccagcac gccgtctttc
      601 cacggcggcc cgatgcgcag ccggcggtac gcatcgacga gccgcgtcgc gaacggctcg
      661 gccacggact cgtgcacgat gagccggcga agcgtcgtgc agcgctgccc ggccgtgccg
      721 gccgcggcga acaccacggc ctcgagggcg agatcgaggt cggccgattg cgtgacgatg
      781 acgccgttgt tgccgccgag ctcgagcagc gaccgcccga gccgggccgc gaccgcgggc
      841 gcgagggcgc gccccatcgc gcagcttccg gtcgccgaga ccagcggcag gcggcgatcc
      901 tccgccatcc atcgcccggc ctcgctgccc gcgacgcaaa ggttccagac ggcgggcgcg
      961 ccgcattcgg ctagcacgcg ttcggcgatt cgttgcacgg cgaccgccgt cagcggcgcc
     1021 tgcggcgacg gtttccagat caccgtgtcg ccgcaaacgg cagcgagcgt cgcgttccag
     1081 gcccacaccg cgaccggaaa gttgaaggcc gtgacgacgc cgacgggccc caacggatgc
     1141 cactgctcga acagccggtg ctgccgacgc tcgctcgcga tcgtcaagcc gtacaactgg
     1201 cgcgacaggc ccgccgcgta gtcgcacatg tcgatcatct cctggacttc gccggccgct
     1261 tccgagctga tcttgccgac ttcgagcatc acgagccgcg cgagatcgct cttgtgctcg
     1321 cgcagcgcga caccgaggcg ccgaacggcc tcgccgcgct cgggcgcagg cacgaggcgc
     1381 caggcgcgga acgcctccga agcgcgcgcg acgacgcggt cgtagtccgc tcgcgcggcg
     1441 gtgcgcacgc gcgcgatcgg atcgccggtc gaggggttga tggactcggt gatggcgccc
     1501 ggcggcgcag cggccttcgc gcttccgatt gcgctcatcg aggcggcctt cgcgcctccg
     1561 atggggccgg gcggggcggc ggctttgacg ccttcaatgc cgccggctgc ggcgctttcg
     1621 cacgccgcga cgccgtcgtt gacgtcgccg attcggattc cgagccgggt cagcgcttcc
     1681 tcatacatga cgttgtctcc ttcgaccgtc gcggaggcgg gcgcgatgcc cgattcgctc
     1741 cgccttgccc ggcgccgtgc gcctttatgc ctctgcgccc ggcacctcta tgcctctgcg
     1801 cgaggcgcgc gcggcttgcg ccgacgagcg ggctcgcgct agccggccgc gagcgcggcc
     1861 tcgaagatgt cgaccgccgt atcggcgtcg cgttcggtga cgatcagcgg cgggcagaac
     1921 cgaaccgtgc tctcgccgca gccgagcagc aacagcccct gctcgaaaca ggcgcggacg
     1981 atgcgattgc gctcgtccgg cgcggggcgc atcgagcgcc gatcgctcac gatctcgacg
     2041 ccgagcatca agccgacgcc ccgcacctcg gcgatcgtgt cccgctccgc ggcgagcgtt
     2101 gtgagacgcg cgcgcaggta gtcgcccgtt cgccgagcgt tctcgatgag cccgcgctcg
     2161 agcagatcga tcgtcgcgag cgcggcgacg cacgagacgg ggttgccgcc gaacgtgctg
     2221 ccgtgcgagc ccggcggcca atccatgatg tcggcgcgcg cgatgatcgc gccgagcggc
     2281 atgcccgagg cgatgccctt ggcgaggcaa acgatgtcgg gcacgacgcc ggtgtgctcg
     2341 atcgcgaaca tgcggcccgt gcggcccatg ccgctctgca cctcgtccac gacatagcag
     2401 atgccgtgac gctcggcgag cgccttgaga cgcggatgaa agtccggcgg cggaacgatg
     2461 tagccgcctt cgccctggat cggttcgacg acgaacgccg cgacctcctc gggcggcacg
     2521 gtgcggccga acacgtcgcg ctcgatctgc tgcatgcact cgtcgacggt cggtccgcgc
     2581 agcggattcg ggaactcgac gtgcgtgact tccggaatca gcggcgcaaa gcccgcgcgc
     2641 tgcgtcgcct tgctgcctgt cagcgcgagc gctcccatcg tcctgccgtg gaatgcgccg
     2701 aagaacgcga tcatgtgctt gcggccggtg tgaaagcgcg cgagcttgaa ggccgcttcg
     2761 atggcctcgg cgcccgagtt cgtgaagaac acgcgtttcg gcacgtcgcc aggcgcgatc
     2821 tccgcgagct tcttcgcgag gtcgcgctgc ggcgcatagt agaagtccgt gccggacatg
     2881 tgcagcagct tcgccgcctg gcgctggatc gcctcgacca cctgcgggtg acagtgaccg
     2941 gtcgagcaga cggcgatgcc cgccgtgaaa tcgaggaacc ggttgccgtc gacgtcctcg
     3001 acgacggcgc cctgggcgcg ctcgacggcg agcgggtata cgcgcgtata cgacggcgac
     3061 gtgtaacgct cgtcgctctc gatcagcgcc ctcgcccttg gtccgggcag gggcgtcacc
     3121 aagcagggta cggagctttg cgtattctcg ctgttcaatt gcgccgctcc ttccaaagga
     3181 ctcgagagcc atttcaatcc caatggacgg agcaacacaa gcgctcggtt ctcgcgactg
     3241 tccggcgaag gtggccgccg ctgatcgacg aattcgtgga caagaccttc gagggctgtc
     3301 gccg
//
LOCUS       DASUJG010000367         2074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1696466, whole genome shotgun sequence.
ACCESSION   DASUJG010000367 DASUJG010000000
VERSION     DASUJG010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1696466"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1601
                     /locus_tag="VFX38_07845"
     CDS             <1..1601
                     /locus_tag="VFX38_07845"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEX5314796.1"
                     /translation="YDSEAVAILQQNLTATQLTGSDVTGGGASSQSGVVELELEGKNP
                     YQIENVLDAITDQYVKENIAAMAAQAKKSLQFVKEQLPIVNRKQIAAMTGLAEYEAKH
                     GVVNLSAQTQLVLQQIGTYESQLTQLELERVTIAQQYTSNFPGYAAILSQEQAIRQKI
                     AALNDELRQVPQQAQGYVELQRDATVYGELYQTLLTTEQSLEISQAGTVGTARIVSHA
                     LTPYQADWPLPVLVIAIGVILGLIFGVIAVLLKSVLTRGVFDATALEQSFGLPVYAII
                     PHSKKEAEVRRRATKKKSHRLDILARAAPNDPAVEAMRSLRTSINFALWDSPRKLITF
                     SGTSPGVGKSFLSANMAHVLAAADARILVIDADMRKGHLHRYFGLEQEQGLSQILTGQ
                     LRIEQCIRRGVDGTAVDFLPCGPYPPGVFELVAGSRFQETVDECAALYDYVLIDVPPV
                     LAVAEGILIARLASANFLVVKAGGQTEREIQLSLERMRQNGVRLLGFVFNDLTPRAAS
                     ATFGRYYGGYGYAYEYRKTETNNA"
     gene            1849..>2074
                     /locus_tag="VFX38_07850"
     CDS             1849..>2074
                     /locus_tag="VFX38_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014595002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5314797.1"
                     /translation="MKRILVTGGAGFIGSALVRFLIRETDHEVVNLDALTYSGNLANV
                     AEVADHPRYRFVRLDLRDPAALGPVFAQAAP"
BASE COUNT          398 a          638 c          659 g          379 t
ORIGIN      
        1 tatacgactc cgaagccgtt gccatcctcc agcagaacct gacggcaacg caattgactg
       61 gttctgacgt cacgggcggc ggcgcgagtt cgcaatcggg tgttgtggag ctcgaactgg
      121 aaggcaagaa cccatatcag atcgagaacg ttctcgatgc aattaccgat caatatgtca
      181 aggaaaatat tgcggcgatg gcggcgcagg cgaagaagag cctgcagttc gtcaaggagc
      241 agttgccgat cgtcaaccgc aagcagattg ccgccatgac cgggctcgcc gaatacgaag
      301 caaagcacgg ggtggtgaat ttgagtgcgc agacacagct cgtgctgcag cagatcggaa
      361 cctatgaatc gcagcttacc caactcgaac tcgagcgggt caccattgca cagcaataca
      421 cgtcgaactt tcccggttac gccgcgatcc tctcgcagga gcaggcgatc cggcagaaga
      481 tcgctgcgtt gaacgacgag ctgcgccaag tgccgcagca ggcgcagggt tacgtcgagc
      541 tgcagcgcga tgccactgtc tatggggagc tctaccagac cttgctcacg acggagcaga
      601 gtctcgagat atcacaggcg ggcaccgtcg gtaccgcgcg catcgtgagc cacgccctga
      661 cgccctacca ggccgattgg ccgcttcccg tgctggtgat cgcaatcggg gtcattctgg
      721 gacttatctt cggcgtgatc gcggtccttc tgaagagcgt gctcacgcgt ggcgtgttcg
      781 atgcaaccgc gctggagcag agcttcggcc tgccggtcta tgcgatcatc ccgcacagca
      841 agaaagaggc cgaggtgcgt cgccgggcga cgaaaaagaa gagccaccgc ctcgatatcc
      901 tcgcgcgcgc cgcgccgaac gatccggcgg tcgaggcgat gcgcagcctc cggacgagca
      961 tcaatttcgc tctctgggac agcccgcgaa agctgattac cttcagcggc acgtcgccgg
     1021 gcgtggggaa aagctttctc agcgcgaaca tggcgcacgt tctcgcagcg gcggatgcac
     1081 gcattctcgt tatcgatgcg gacatgcgca aggggcatct tcatcggtat ttcggtctgg
     1141 aacaggagca agggctgtcg cagatcctga cgggtcagct gcgcatagag cagtgcatcc
     1201 ggcgcggcgt ggacgggacc gcggtggact tcctgccctg cggaccgtat ccgccgggtg
     1261 ttttcgagtt ggttgccggc agccgctttc aggagacggt ggacgaatgc gccgcgctct
     1321 acgattacgt gctcatcgac gtgccgccgg tgctcgccgt cgccgagggg attctgattg
     1381 cgcgcctcgc gagtgcaaat tttctggtgg tcaaggccgg cggtcaaacg gagcgcgaga
     1441 tccagctgtc gctcgagcgg atgcgccaga acggcgtgcg cctgctcggg ttcgtgttca
     1501 atgaccttac gccgcgcgcg gcgtccgcaa ctttcgggcg ctactacggc ggttacggct
     1561 atgcctacga ataccggaaa acggaaacca acaacgcctg aggccacagg attccgatcc
     1621 cgcgtggcgg cggagccggc gatggagctc ggccttctcg ccggccttgc gcgctgcgaa
     1681 gcgggagtga ccgcgactcc gtaagccggc ctcgcggacc cagcatcgcg acttgagcct
     1741 gcctggcgct ctggcctgca cgaaattccc tgatgggccg tctgaactcc acggagtgct
     1801 tttagtgacg acgaaatccc tgtacccgcg attgccgcaa gccgcgccat gaagcgaatc
     1861 ctggtgacgg gcggtgccgg attcatcggc tcggcgctcg tgaggtttct cattcgcgag
     1921 acggatcacg aagtcgtcaa cctcgacgcg ctgacgtatt ccggcaatct tgcgaacgtc
     1981 gccgaggtcg cggatcatcc gcgctatcgc ttcgtgcgcc tggaccttcg cgaccctgcg
     2041 gccctcggcc ccgtattcgc gcaagcggcc ccag
//
LOCUS       DASUJG010000368         2155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_839968, whole genome shotgun sequence.
ACCESSION   DASUJG010000368 DASUJG010000000
VERSION     DASUJG010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839968"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1105
                     /gene="trmFO"
                     /locus_tag="VFX38_07855"
     CDS             <1..1105
                     /gene="trmFO"
                     /locus_tag="VFX38_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEX5314798.1"
                     /translation="VMRAADKHRVPAGSALAVDREAFAAEVSRTIEQHPNVTVVRERV
                     DSLPDHPAIIATGPLTGSKLAASISAETGEDALAFFDAIAPIVHRDSIDMDVAWMAAR
                     WDKGGKDYINCPLDKPQYEDFVAALIDGEKMPFHDWERDTPYFEGCMPIEVMAERGPE
                     TLRYGPMKGVGLDNPRTGRWPYAVVQLRQDNALGTLWNMVGFQTKLKHGEQVRIFRTI
                     PGLENAEFARLGGIHRNSFINSPRLLDRELRLKSRPNIRFAGQITGCEGYVESAAVGI
                     LAARFAAAEIKGQSLDEGLSLPPPETALGALLNHITGGADAETFQPMNVNFGLMPPLE
                     GRSKRADRKKAYTDRARAAFGEWLEQFRVIEPA"
     gene            complement(1102..1557)
                     /locus_tag="VFX38_07860"
     CDS             complement(1102..1557)
                     /locus_tag="VFX38_07860"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314799.1"
                     /translation="MLRFFAGAAACFLLMTGAFLIWQSRAEQGPGIPGAPPARTYTAS
                     MLQAPEASTKSREERRFGRADKNKNGKIESEELLGPRRKAFAKLDTNGNGSLAFEEWA
                     HTTIDKFKGADKDRNGLLTAAEYATTAPPPPKHRTCSCGVPGPIARSDD"
     gene            complement(1626..>2155)
                     /locus_tag="VFX38_07865"
     CDS             complement(1626..>2155)
                     /locus_tag="VFX38_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314800.1"
                     /translation="LAWWRERLEELDAGVPTPPEPRLQAIERELLPRGVSGRDLGGLE
                     QGWLRLFDPFPWTIETSEAIWLRGNLLFGLGARVLGQAGEAIQTAGGLWALVDSARHC
                     SDSDSRQMLIGQARSFAKRIGGTRFAAHLRPLSMLAALAVRDCRHGEPFEAEGTAGRV
                     AAMLRHRLGGRIPRP"
BASE COUNT          367 a          785 c          670 g          333 t
ORIGIN      
        1 ggtcatgcgc gcggccgaca agcatcgtgt gccggcagga tcggcgcttg ccgtcgaccg
       61 cgaagccttc gccgccgaag tttcgcggac gatcgagcag catccgaacg tcacggtcgt
      121 gcgggaacgc gtcgattcgc tccccgatca tccggcgatc atcgcaacgg gaccgctcac
      181 aggctcgaag ctcgcagcct ccattagcgc cgagaccggc gaagacgcgc tcgccttctt
      241 cgacgcgatc gcgccgatcg tccaccgcga cagcatcgac atggacgtcg cctggatggc
      301 cgcgcgctgg gataaaggcg gcaaggatta catcaactgc cccctcgaca agccgcagta
      361 cgaggatttc gtcgcggcgc tgatcgacgg cgagaagatg cccttccacg actgggagag
      421 ggacacgccc tacttcgagg gctgcatgcc gatcgaggtg atggccgagc gcggccccga
      481 gacgctgcgt tatggcccga tgaagggcgt cggcctcgac aatccgcgca ccggccgctg
      541 gccctacgcc gtcgtccagc ttcgccagga caatgcactc ggcaccttgt ggaacatggt
      601 cggcttccag accaagctaa agcacggcga gcaggtgcgc atcttccgca ccattcccgg
      661 cctggaaaat gccgagttcg cgaggctcgg cgggatccac cgaaacagct tcatcaactc
      721 gccgcggctg ctcgaccgcg agcttcggct caaatccaga ccgaacatcc gcttcgccgg
      781 ccagatcacc ggctgcgaag gctatgtcga aagcgctgcc gtcggcatcc tagccgcccg
      841 cttcgccgcc gccgaaatca agggacagtc cctcgacgag ggactatccc tcccaccgcc
      901 cgaaaccgcg ctcggcgccc tgctgaacca catcaccggc ggcgccgacg ccgagacctt
      961 ccaaccgatg aacgtcaact tcggcctcat gccgcccctt gaaggccgct cgaaacgcgc
     1021 cgaccgcaag aaagcctaca ccgaccgcgc ccgcgcggct ttcggcgaat ggctggagca
     1081 gtttcgcgtc atcgaaccgg cctagtcgtc actgcgcgcg attggtcccg gtaccccgca
     1141 gctgcacgtc ctgtgcttcg gcggcggcgg ggcggtggtc gcgtattcgg cggcggtgag
     1201 gaggccgttt ctgtccttgt cggcgccctt gaacttgtcg atcgtcgtgt gcgcccattc
     1261 ctcgaaggcc agcgaaccgt tgccgttcgt atccagcttg gcgaacgctt tccgccgcgg
     1321 gccgagcagc tcctcggatt cgatcttgcc attcttgttc ttgtccgcgc ggccgaagcg
     1381 ccgctcctcg cggcttttcg tgctggcctc cggagcctga agcatcgacg cagtgtaggt
     1441 gcgcgccggc ggagcgccgg ggatccccgg gccctgctct gccctcgact gccagatgag
     1501 gaaggcgccg gtcatcagca ggaagcacgc cgcggcaccg gcgaagaaac gaagcatcgg
     1561 caaatctcct tcatttcagg agattcggca caccccatgc tccgcaaccg agcggttcgt
     1621 gtcaactacg gtcgcggaat gcgcccgccg agccgatggc ggagcatcgc cgccacgcgc
     1681 cccgccgtgc cttccgcctc gaacggctcg ccgtggcggc aatcgcgcac cgcgagcgcc
     1741 gcgagcatcg aaagcgggcg aagatgcgcc gcgaaccgcg ttccgccgat ccgcttcgcg
     1801 aacgagcgcg cctggccgat cagcatctgc cgcgagtccg agtcgctgca gtgccgcgcc
     1861 gaatcgacca gcgcccagag gccgcccgcc gtctggatcg cctcgcccgc ctggccaaga
     1921 acgcgcgcgc ccagcccgaa caacagattg ccgcgcagcc agatcgcctc gctggtctcg
     1981 atcgtccacg gaaatgggtc gaacagccgc agccagccct gttccaggcc accgaggtcg
     2041 cgtccgctga ctccgcgcgg caggagctcg cgctcgattg cctgcagccg cggctcgggc
     2101 ggggtcggga ctcccgcgtc cagctcctcc aggcgctcgc gccaccacgc aagac
//
LOCUS       DASUJG010000369         5675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1330638, whole genome shotgun sequence.
ACCESSION   DASUJG010000369 DASUJG010000000
VERSION     DASUJG010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5675
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1330638"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VFX38_07870"
     CDS             complement(<1..400)
                     /locus_tag="VFX38_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314801.1"
                     /translation="MRPELAARASALALILFPLAVWLGEGRIENRYLVAILLALIALR
                     LALAAFARRRRLWAAIAALALAGALVCALLPWSFPVPLRYYPVAIGGAFAALFLFSSL
                     SARPLVERIARLSDPDLQAAAIAYCRRLNWL"
     gene            complement(390..647)
                     /locus_tag="VFX38_07875"
     CDS             complement(390..647)
                     /locus_tag="VFX38_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003849951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX5314802.1"
                     /translation="MNPEALHARLRAILVEQFEIAPERITPEARLYEDLGLDSIDAVD
                     LVIHVQELTGRKIRPQEFREVRTVSDVERTIESLLGTTNEA"
     gene            complement(644..931)
                     /locus_tag="VFX38_07880"
     CDS             complement(644..931)
                     /locus_tag="VFX38_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017429812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEX5314803.1"
                     /translation="MNAEAAASGLIEELKALIIRTLDLEDVTPADIDAEAPLFGEGGG
                     LGLDSIDALELGVALQKHYHIPIEEEAGSYRDHFGSVAALARFVQSRSGRP"
     gene            complement(928..1710)
                     /locus_tag="VFX38_07885"
     CDS             complement(928..1710)
                     /locus_tag="VFX38_07885"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5314804.1"
                     /translation="MRESRLAGCWRAAASGLCFLGYWTGCLLVAPTALLAIVMLSRSA
                     FTRARRTRRLASASFRLLIRVCRILGIVECEIAGREWLAAAPGRLVLANHPTFLDVIV
                     LLALLPEANCVVKHSLRRHPVLAPFVRACGYLANDTEALAFLAACRDAKARGETLIVF
                     PEGTRSTAGAPLHFQRGAAQIALRAGMEILPVTVYCDPPFLTRQTRWYRAPQRRVRHL
                     VKFHPPVAAEGFAPVAGLEPPLAARRLTRGLQSYFQNELLSP"
     gene            complement(1703..2455)
                     /locus_tag="VFX38_07890"
     CDS             complement(1703..2455)
                     /locus_tag="VFX38_07890"
                     /inference="COORDINATES: protein motif:HMM:NF025100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HEX5314805.1"
                     /translation="MTTGSERFRNGADGALAIARWQAWTPDAREDGDWAAWFEGRRNA
                     QEAAQPDVSFLPAMLRRRLERSGRMSLAVAAGALGKDEKMATIFASRHGPFHRTMELL
                     AALARDEPISPTLFSLGVHNSVAGLFSIARADRSTHTSVAAGAATLAAAFWEAAGQLA
                     GGAPAVLIVYVSEPPPAMYQPYIGAEEPLLAFAAVLEPARADVPAWRLRREDGIAMGE
                     DPALALLRSLCHGSDRAVLAPGWLLARASAHA"
     gene            2612..3400
                     /locus_tag="VFX38_07895"
     CDS             2612..3400
                     /locus_tag="VFX38_07895"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5314806.1"
                     /translation="MTEWDAAGYFRMSGLQQAMAEEVLALLPLARARRVLDVGCGDGK
                     LSARIAHEAPRAELIGVDRSRAMIAFAAAHFDPARHSNLAFAVADARALPFRGRFDLA
                     VSFNALHWVPDQAAALRSLRAALESGGRAQLRLVTAGERKSLESIVEETRESERWRGH
                     FANFSDPYLRLTPAEYAALAESAGLRVLGAATALKTWDFGSRAAFFDFCAVGLVAWTE
                     RLPAEDRAGFIGEALDRYAALAGSRANAASTFHFYQSDFTLGRE"
     gene            3504..4034
                     /locus_tag="VFX38_07900"
     CDS             3504..4034
                     /locus_tag="VFX38_07900"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX5314807.1"
                     /translation="MSDEDAARVLFGGEYLRLLRRRHWEYAERTNSSGVVVIVAVTPE
                     KKLLLVEQFRWPVASAVIELPAGLVGDVDGDEDLSAAARRELIEETGFEAGEMRVLLS
                     GPVTAGLSNEVTHFCLARDLRRVGEGGGDASEDIRVHEVALAEVPGWLASRPPALLVD
                     PKTYIGLWLYAAYAGP"
     gene            4062..>5675
                     /locus_tag="VFX38_07905"
     CDS             4062..>5675
                     /locus_tag="VFX38_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX5314808.1"
                     /translation="MKVSIAAILLAFFTAPVMAASPHPFDVHDLVMMDRVGDPVLSPD
                     AGTAAFSVRETDYAANKGVTSVWVLNLRDPDAKPEKIREGASPQFSADGTLFFLADDK
                     AGITQLWRRSGNSAEQASELPISINNFRISPDGRHVLVSVDVFMDCKDLACTRAKLDA
                     RAKDKATGRLYHKLFVRHWNEWMDGRRSQLFIAMLGSYGKLEGAPIWLTKNVDADIPS
                     KPFGDNTDYGFSPGGKTVYFDAKPGGSSEAWSTDFNVYSVPADGSAAPVNLTSDNEAW
                     DAFPLASADGKTLYYLAMKTPGFESDRFAIMARDLATGSTREVDPDWDRSAGPLQLSA
                     DGKTLYTTADDEGNHRLFAVDMASGKVRNLSGDGTVTGFAAANGKLLVARQDFKHPTD
                     LYAANANGGNFHQLTHFNAARLANIEFADWQWFSFKGWNGDTVQGYVMQPVGYVKGRK
                     YPIAFLIHGGPQGSWTDDFHYRWNPETYAGQGFAVVAINFHGSTGYGQAFTDSISQHW
                     GDRPLIDLQKGWAAALTKFPFLAADRACAL"
BASE COUNT          975 a         1868 c         1914 g          918 t
ORIGIN      
        1 agagccagtt caggcggcgg caataggcga tggccgcagc ttgcagatcg ggatcgctca
       61 ggcgcgcgat gcgctcgacc agcggacgcg cgctcagcga ggagaacagg aatagcgccg
      121 cgaacgcgcc gccgatcgcg accgggtagt agcgcagcgg taccggaaag ctccagggca
      181 ggagcgcgca gacgagtgcg ccggcgagcg ccagcgcggc gatcgcggcc cacaggcgcc
      241 gcctgcgggc gaaggccgcg agagcgaggc gcagggcgat cagcgcgagc agaattgcga
      301 ccagatagcg gttttcgatt cgcccctctc ccagccatac ggcgagggga aagagaatca
      361 atgccagcgc gcttgcgcgt gccgccagct caggcctcat tggtcgtgcc gagcaggctc
      421 tcgatcgtgc gctcgacgtc cgagacggtg cgcacctcgc gaaactcctg cgggcggatt
      481 ttccgtccgg tcaactcctg cacgtggatc acgaggtcca ccgcgtcgat gctgtcgaga
      541 ccgaggtcct cgtagagacg cgcctcgggc gtgattcgtt cgggcgcgat ctcgaactgc
      601 tcgacgagaa tggcccgcag gcgcgcgtgg agtgcttccg ggttcacgga cgcccggagc
      661 gtgactggac gaagcgcgcg agcgcggcaa cggagccgaa gtgatcgcgg taggagccgg
      721 cctcctcctc gatcggaatg tggtagtgct tctggagagc gactccgagt tcgagggcgt
      781 cgatggaatc gaggccgagt ccgccgcctt ctccgaagag cggcgcctcg gcgtcgatgt
      841 ccgccggcgt cacgtcttcc agatcgagcg tccggatgat gagcgctttc aattcttcga
      901 tcaggccgct ggcggcagct tcggcattca tggagaaaga agctcgttct gaaaataaga
      961 ttgtagcccg cgggtaagcc gccgagccgc taggggcggc tcgagacccg ccaccggggc
     1021 gaaaccctcg gcggccacgg gagggtgaaa ttttaccaag tgacggaccc ggcgttgcgg
     1081 cgcccgatac cagcgggtct gcctggtgag aaagggcggg tcgcaatata ccgtcaccgg
     1141 caggatctcc atgccggcgc gcagcgcgat ctgggccgcg ccgcgctgaa agtgcagcgg
     1201 cgcgccggcg gtgctgcggg tcccttccgg aaagacgatg agggtctccc cgcgcgcctt
     1261 ggcatcgcgg caggcggcga gaaaggcgag cgcctcggtg tcgttcgcaa gatagccgca
     1321 ggcgcgcacg aagggcgcga gcaccgggtg acggcgcagg gaatgcttga cgacgcagtt
     1381 ggcctcggga aggagcgcga gcagcacaat tacatcgagg aacgtcgggt gattagcgag
     1441 cacaagccgt cccggcgcgg cggcgagcca ttcgcggccg gcgatttcgc actccacgat
     1501 tccgagaatc cggcaaaccc gaatcaggag ccggaaactg gcgctcgcga ggcggcgtgt
     1561 gcgccgcgcc cgggtgaacg cgctgcggga aagcatgacg atcgcgagga gcgcggtcgg
     1621 cgcgaccaga aggcaaccgg tccagtagcc gagaaagcag aggcccgagg ccgccgcacg
     1681 ccagcagccc gcgaggcgcg attcacgcat gcgcggaagc acgcgcgagc agccagcccg
     1741 gcgcgagcac ggctcgatcg ctcccgtggc aaagcgaacg gagcaatgcg agcgccggat
     1801 cctctcccat cgcgatgccg tcttcgcgcc gcagcctcca ggcgggaacg tccgcccgcg
     1861 ccggttcgag cacggcggcg aaggcaagca gaggttcttc cgctccgatg tagggctggt
     1921 acatcgccgg cggcggttcg ctcacataga cgatgagcac cgccggagct ccgccggcga
     1981 gttgcccggc ggcctcccag aaggcggcgg caagcgtcgc cgccccggcg gcgaccgaag
     2041 tgtgggtgct ccggtccgcc cttgcgatcg agaagagccc cgcgacgctg ttgtgcaccc
     2101 cgaggctgaa gagcgtcggc gaaatcggct cgtcgcgggc gagtgccgcg agcagttcca
     2161 tcgtgcgatg gaagggcccg tgccgcgagg cgaaaatcgt cgccatcttc tcgtccttgc
     2221 cgagcgcccc cgcggcgacg gcgagcgaca tccgccccga gcgctcgaga cgccggcgga
     2281 gcatcgccgg aagaaagctt acgtcgggtt gcgctgcttc ttgggcgttg cgccggcctt
     2341 cgaaccaggc cgcccagtcg ccgtcctcgc gcgcatcggg cgtccaggcc tgccagcggg
     2401 cgatggcgag cgcgccgtcg gcgccgtttc ggaaccgctc cgagcccgtc gtcacgattg
     2461 cctttccctc cggcccgaag cacgggccat tgtcctgctc ccgccaaaat actgcatgac
     2521 cgatctgcgg gcaagcgcgg gaggagacgc aaggcgcgcg ggcgatggga tatgctcgcg
     2581 ccttcccgcg ggagcggatc cgggccgaag catgacggaa tgggacgcag ccggctattt
     2641 ccggatgtcg ggcttgcagc aggcgatggc cgaagaggtg ctcgctcttt tgccgctcgc
     2701 gcgtgcccgc cgcgtgctcg acgtcggctg cggcgacggc aaattgagcg cccggatcgc
     2761 gcacgaagcc ccgcgggcag agttgatcgg cgtggatcgt tcgcgcgcga tgattgcctt
     2821 cgccgcggcg catttcgacc cggcgcggca ttccaatctc gcctttgccg ttgcggacgc
     2881 gcgcgcgctt ccgtttcgcg gccgcttcga tcttgcggtt tccttcaatg ccctgcactg
     2941 ggtgcccgat caggcggcgg cgctccgttc gctccgcgcg gcgctcgaat cgggcgggcg
     3001 ggcgcaactg cgcctcgtca cggcgggcga gcgaaaaagt ctcgaaagca tcgtcgagga
     3061 aacgcgcgaa tcggaacgct ggcgcggaca tttcgcgaat ttctccgatc cctatctgcg
     3121 cctcacgccc gcggaatatg ccgcgctcgc cgaaagcgca ggcttgcgcg tgctcggcgc
     3181 cgcgaccgcg ctcaagacct gggatttcgg atcgcgcgcc gcattcttcg acttctgtgc
     3241 ggtcggcctc gttgcctgga ctgaacgcct gcctgcggag gaccgcgccg gattcatcgg
     3301 cgaggcgctc gatcgttacg ccgcgcttgc gggcagccgc gcgaacgccg caagcacgtt
     3361 tcacttctac cagtccgact tcacgctcgg gcgcgaatga actccttgcg gatttgccgc
     3421 cgtcgcccgc ccggccgata tactggacga cgcagcgttt ttcgacggga acggacaatg
     3481 cctccggcca aggcgaggcg cacatgagtg acgaggatgc ggcgcgcgtt cttttcggcg
     3541 gcgaatacct gcgcctgttg cgccgccgcc attgggaata cgcggaacgc acgaattcga
     3601 gcggagtcgt cgtcatcgtc gcggtcacgc ccgagaaaaa gctgctgctc gtcgagcagt
     3661 tccgctggcc ggtcgcgagc gccgtgatcg agcttcccgc cggcctcgtc ggcgacgtcg
     3721 acggcgacga agacctttcc gcagccgccc ggcgcgagtt gatcgaggaa accggtttcg
     3781 aggcgggcga aatgcgggtg ctgctttcgg gtcccgtcac cgctgggctc agcaacgagg
     3841 tcacgcactt ctgtctcgcg cgcgatctcc ggcgcgtcgg cgagggcggc ggcgatgcga
     3901 gcgaggacat ccgcgtgcac gaagttgcgc tcgccgaggt acccggttgg ctcgcctcgc
     3961 gcccgcccgc cctgctcgtg gaccccaaga cctacatcgg cctgtggctg tacgcggcgt
     4021 acgccgggcc ctgattcggc aatggaatcg gaggaaatgg catgaaagtc tcgattgcgg
     4081 ctatcttgtt ggcgttcttc acggcgccgg tcatggccgc gtccccgcat cccttcgacg
     4141 tgcacgatct cgtgatgatg gatcgcgtcg gcgatcccgt cttgtcgccc gatgcgggga
     4201 cggccgcctt cagcgtccgc gaaaccgatt acgccgcgaa caagggcgtg acgagcgtgt
     4261 gggtgctgaa tcttcgcgac cccgacgcca aaccggagaa aatccgcgag ggcgcgagcc
     4321 cgcaattttc cgccgacgga acgctctttt ttctcgccga cgacaaggcc ggtatcaccc
     4381 agctctggcg gcgaagcggc aactccgccg agcaggcgag cgagctgccg atctcgatca
     4441 acaacttccg catctcgccc gacggcaggc acgttcttgt gtccgtggac gtattcatgg
     4501 actgcaagga tctcgcctgc acccgtgcga agctcgatgc gcgcgccaag gacaaggcga
     4561 ccggcaggct gtatcacaag ctcttcgtgc gccactggaa cgagtggatg gacgggcggc
     4621 gctcgcagtt gttcatcgcg atgctcggca gctacggcaa actcgaaggc gctccgatct
     4681 ggctgaccaa gaacgtcgat gcggacatcc cctcgaagcc cttcggcgac aacaccgact
     4741 acggtttttc gcccggtggc aagacggtgt atttcgatgc caagccgggc ggttcgagcg
     4801 aggcctggtc cacggatttc aatgtttatt cggtgccggc tgacggctcc gccgcgccgg
     4861 tgaatcttac ttccgacaac gaggcctggg acgcctttcc gctcgcatcc gccgacggca
     4921 agacgctcta ctatctcgcg atgaaaacgc cgggattcga gtccgatcgc ttcgcgatca
     4981 tggcccgcga tctcgcaacc ggctcgaccc gcgaagtgga tccggactgg gatcgctcgg
     5041 cgggcccgct gcaactttcc gcggacggaa agacgcttta caccacggcc gacgacgagg
     5101 gcaatcaccg gctgtttgcc gtggacatgg caagcggcaa ggttcgcaat ctcagcggcg
     5161 atggcaccgt cacgggcttt gccgctgcga acggaaaact cctcgtcgca cgccaggact
     5221 tcaagcatcc gaccgatctg tatgcagcca atgcgaacgg cgggaatttc catcaactca
     5281 cacatttcaa cgccgcgcgc cttgcgaaca tcgagttcgc cgactggcaa tggttcagct
     5341 tcaagggctg gaacggcgat accgtgcagg gctacgtcat gcagcccgtc ggctacgtca
     5401 agggaaggaa atatccgatt gcctttctga ttcacggcgg cccgcaaggc tcctggaccg
     5461 acgactttca ctaccgctgg aatcccgaaa cctacgccgg gcagggcttc gccgtcgtgg
     5521 cgatcaattt ccacggctcg accggctacg gacaggcctt caccgattcc atctcgcagc
     5581 actggggcga ccgcccgctg attgacctgc aaaagggctg ggccgccgcg ctcacgaagt
     5641 tcccgttcct cgccgctgat cgtgcctgtg cgctc
//
LOCUS       DASUJG010000370         2581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1330801, whole genome shotgun sequence.
ACCESSION   DASUJG010000370 DASUJG010000000
VERSION     DASUJG010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1330801"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VFX38_07910"
     CDS             complement(<1..399)
                     /locus_tag="VFX38_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008632385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX5314809.1"
                     /translation="MMMRAMVLEKGARRLLEREVPRPEPQAGEILVRVFACGVCRTDL
                     HIVDGELAPPAWPVIPGHEIVGEVAALGKGVEEFAMGERVGLAWLAGADGDCAFCRRG
                     EENLCPNAKFTGFTRPGGYAEYTTARADFAY"
     gene            complement(476..1459)
                     /gene="lipA"
                     /locus_tag="VFX38_07915"
     CDS             complement(476..1459)
                     /gene="lipA"
                     /locus_tag="VFX38_07915"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX5314810.1"
                     /translation="MPVVESGRKFTTPQGFTAIKNGIRARAAAPTPDIFLRKPAWLRV
                     KLPSGDGYEAVKTNVRRHRLATVCEESMCPNIAECWNAGTATIMIMGSVCTRACRFCA
                     VDTGNPRGWLDSDEPAQVADSVRLMGLKYVVMTSVDRDDLPDGGAGHYAACVRAIRRA
                     NPETAVEALTPDFRGVAEHVAKVVDSGLQVYAQNLETVERLTHEVRDPRAGYRQTLDV
                     LAFAKRHKPDVLTKTSLMLGLGERDEEIAQALDDCRAAGVDIVTFGQYLRPTKNHLPV
                     VRYVPPEDFARYREWGLERGFLEVVAGPLVRSSYRAERVFERNNAGLGTRD"
     gene            1598..>2581
                     /locus_tag="VFX38_07920"
     CDS             1598..>2581
                     /locus_tag="VFX38_07920"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018623542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEX5314811.1"
                     /translation="MPRPDLSFLDRAVEELKTEGLYKAERILAGPQGAEIEVGGRRVL
                     NLCANNYLGLAGHPELVKAAQNALDRYGYGMASVRFICGTQDVHKALEAKLAGFLGVD
                     DAILYSSCFDANGGLFETLLDENDTIISDALNHASIIDGVRLCKAKRLRYANNDMAEL
                     EARLKEAKDSRLRLIATDGVFSMDGIVADLPAICDLADRYEALVMVDDSHATGFMGAG
                     GRGSHEYRNVMGRIDLITTTFGKALGGASGGATAGRREIIGWLRQRSRPYLFSNSLAP
                     SITATTLHVLDMLESGSELRERLHDNARHFRTGMSALGFDLVPGEHPIIPIM"
BASE COUNT          444 a          898 c          796 g          443 t
ORIGIN      
        1 gtaggcgaaa tccgcgcgcg ccgtcgtgta ttcggcgtat cccccggggc gcgtgaagcc
       61 ggtgaatttt gcgttcgggc agagattctc ctcgccccgc cggcagaatg cgcagtcgcc
      121 gtcggcgcct gcgagccagg cgagtcccac ccgttctccc atcgcaaatt cctcgacgcc
      181 tttacccagc gccgcaactt cgccgacgat ctcgtgaccg ggaatcaccg gccacgcggg
      241 cggcgcgagt tcgccgtcca cgatgtgcaa gtcggtgcga cagacgccgc aggcgaacac
      301 gcgcacgagg atttcacccg cttgcggttc gggccgcggc acctcgcgtt cgagcagtcg
      361 gcgcgcgccc ttctccaaca ccatcgcccg catcatcatc gacgtcgccc tgcgctccgt
      421 aggatgcggt gaatccgcga accgcatcaa cccaaaccgc gaccgatgcc ggtccctagt
      481 ccctggtccc caaccccgca ttattgcgct cgaagacgcg ctcggcgcga tagctggagc
      541 gcaccagcgg cccggcgacg acttcgagaa atccgcgctc cagcccccat tcgcgatagc
      601 gcgcgaaatc ctccggcggc acgtaacgga cgaccggcag atgattcttc gtcggccgca
      661 gatactgtcc gaaggtcacg atgtccacgc cggccgcgcg acaatcgtcg agcgcctgcg
      721 cgatttcctc gtcgcgctcg ccgagtccga gcatgaggct ggtcttggtt agcacgtccg
      781 gcttgtgacg cttggcgaag gcaagcacgt cgagcgtctg gcgataaccc gcgcgcggat
      841 cgcgtacctc gtgcgtcagg cgctcgacgg tttccagatt ctgcgcatag acctgcaagc
      901 ccgaatccac caccttggct acgtgctccg ccacgccccg aaagtccggc gtcagcgcct
      961 cgaccgccgt ctcgggattc gcgcgccgga tcgcgcgcac gcaggcggcg taatgccccg
     1021 cgccgccgtc gggaagatcg tcgcgatcca ccgaagtcat caccacatat ttcaggccca
     1081 tcagccgcac cgaatcggcc acctgcgccg gttcgtcgga atccagccag ccgcgcgggt
     1141 tgccggtgtc cacggcgcag aacctgcagg cccgcgtgca caccgaaccc atgatcatga
     1201 tggtcgcggt gccggcgttc cagcactcgg cgatgttcgg gcacatggat tcttcgcaga
     1261 ccgtcgcgag ccggtgccgg cgcacgttgg tcttcaccgc ctcgtagcca tcgccgctcg
     1321 gcagtttcac ccgcagccag gcgggcttgc gcagaaagat gtccggcgtc ggcgccgcgg
     1381 cccgcgcccg gatgccgttc ttgatcgcgg tgaaaccctg cggcgtcgtg aacttgcgcc
     1441 cgctttccac gaccggaatg tcggcgagac gcttcacggg ccggctcgga agaatgcgtg
     1501 catgcgctta ttatcccacc tcgggccggc gttcgggccg cgcgcggctc accggcgccc
     1561 gaaagtataa tgagcgggtt ttccgcagga gtgcagcatg ccgcgaccgg atctctcctt
     1621 tctcgatcgc gccgtcgaag aactgaagac cgaggggctc tacaaggcgg aacgcattct
     1681 cgccggtccg cagggggccg aaatcgaggt cggcggccgg cgcgtgctga atctctgcgc
     1741 caacaactat ctcgggcttg caggtcatcc ggaactcgtg aaggccgcgc agaatgccct
     1801 cgatcgctac ggctacggca tggcatcggt gcgcttcatc tgcggcactc aggacgtgca
     1861 caaggcgctg gaggcgaagc tcgccggctt cctcggcgtg gacgacgcga ttctctattc
     1921 ctcctgcttc gatgccaacg gcgggctgtt cgaaacgctc ctcgacgaaa acgacacgat
     1981 catctccgat gcgctcaatc acgccagcat catcgacggc gtgcgcctgt gcaaggcgaa
     2041 gcgtctgcgc tacgcgaaca acgacatggc ggaactcgaa gcgcgattga aggaagcgaa
     2101 agactcgcgt ttgcgactca tcgcgaccga cggcgtgttt tcgatggacg gaatcgtcgc
     2161 cgatctcccg gcgatctgcg atctcgccga ccgctacgaa gcgctggtga tggtggacga
     2221 ttcacacgcg accggtttca tgggcgcggg cgggcgcggc agtcacgaat accgcaatgt
     2281 catgggccgc atcgatctca tcaccacgac cttcggcaag gccctcggcg gcgcctcggg
     2341 cggcgcgacc gccggacgcc gggaaatcat cggctggctg cgccagcgtt cgcgcccgta
     2401 tctcttttcc aactcgctcg cgccctcgat cacggcgacc acgctgcacg tgctcgacat
     2461 gctcgaatcg ggttcggaac tgcgcgaacg gctgcatgac aatgcgcgtc atttcagaac
     2521 gggaatgagc gcgctcggct tcgatctcgt gcccggcgag catccgatca tcccgatcat
     2581 g
//
LOCUS       DASUJG010000371         4154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_107616, whole genome shotgun sequence.
ACCESSION   DASUJG010000371 DASUJG010000000
VERSION     DASUJG010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107616"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VFX38_07925"
     CDS             complement(<1..296)
                     /locus_tag="VFX38_07925"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011587846.1"
                     /note="adds enolpyruvyl to UDP-N-acetylglucosamine as a
                     component of cell wall formation; gram-positive bacteria
                     have 2 copies of MurA which are active; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX5314812.1"
                     /translation="MNKLLIEGGARLEGEIRISGAKNATLPILAASLLADTPLTLGNV
                     PHLQDVTITVSLLRRMGVEVTVGEHMELEVDSAPIRDFVAPYELVRTMRASILVL"
     gene            complement(293..532)
                     /locus_tag="VFX38_07930"
     CDS             complement(293..532)
                     /locus_tag="VFX38_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HEX5314813.1"
                     /translation="MNRAELENLLTGSFSEAEVAVATADGVHFSARIVTPRFAGHPRL
                     ARHREVYAALGERMGGEIHALTLETLTPEEARERA"
     gene            636..1622
                     /locus_tag="VFX38_07935"
     CDS             636..1622
                     /locus_tag="VFX38_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEX5314814.1"
                     /translation="MPATAPPASAAQLIRSGREVVVIEAAAVAALAARLNETFAAACG
                     LLLACRGRVVTTGMGKSGHIARKVAATLASTGSPAFYVHPGEASHGDLGMITGEDVLI
                     AVSHSGETPEVLAILSLLKRLGAGLIALTGNPASTLARAADCHLDVAVSREACPLNLA
                     PTASTSAALAMGDALALAVSAARGFTAEDFARAHPGGNLGRRLLLRVADLMHRGEAIP
                     LVGEAATLREALVEMSAKGLGMTLVTDSAGQLLGLFTDGDLRRSLDQGAGLASPIADL
                     MTRECTVAAPEMLAGEVLRLMDRRRINGMPVVADGRLVGVFNMHDLLQAGVV"
     gene            1619..2110
                     /locus_tag="VFX38_07940"
     CDS             1619..2110
                     /locus_tag="VFX38_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEX5314815.1"
                     /translation="MNRERLAAVRLLALDVDGVLTDGRVWFFDDGHEAKAFSIRDGHG
                     IKRLLAAGVAVIIISGRRSAASAHRAQELGITAIHEGVSDKAGVLAEAAATAGVALAD
                     CAFMGDDEPDMPALEAAGIALAPADAIPAVRALADWCATSAGGRGAVREACELLLAAR
                     QSA"
     gene            2107..2670
                     /gene="lptC"
                     /locus_tag="VFX38_07945"
     CDS             2107..2670
                     /gene="lptC"
                     /locus_tag="VFX38_07945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEX5314816.1"
                     /translation="MRTLLRRAFAVALVLVAGFTSWLIIANHASVAPPAPAPVSDRSP
                     DYTLRDAVVTRYDPKGARRYVLRAQSIAHLPASGASVFGQPRLDYVAGPGNAWRVTAE
                     RGRLGSDNHLLLLRGKVYGRQLQGDNPLRITTAAAMVQLDRRIVRSRSPVRLWREGFQ
                     TDGIGLDADLTSGVVKLLAEVRSRYAP"
     gene            2660..3187
                     /gene="lptA"
                     /locus_tag="VFX38_07950"
     CDS             2660..3187
                     /gene="lptA"
                     /locus_tag="VFX38_07950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEX5314817.1"
                     /translation="MRRKSRPARRLLAAFLAAVAAAGMLAAAQAPILVSAERSTALLP
                     EGDHAQVTYEGAVVITHGAAVIHGDRAVLYMKNQHLDRAVVSGAPATFIWQPQTGVPV
                     HGEAAIITYLAAADTVVFAGRVKVHRESQTLSAAEARYTLKTGTLSARGGTGTNPERV
                     HVVIPPAPPATAPRP"
     gene            3184..3912
                     /gene="lptB"
                     /locus_tag="VFX38_07955"
     CDS             3184..3912
                     /gene="lptB"
                     /locus_tag="VFX38_07955"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEX5314818.1"
                     /translation="MKRLAAENLHKRFRGREVVKGLSLHVEAGEVVGLLGPNGAGKTT
                     CFYMILGLIRCDAGRITLDERDLTALPVHQRARAGLGYLPQEASIFRHLSARDNVLAV
                     LELNQTKVKRRDRGQRADELLEALGVQHLADTRGDSLSGGERRRVEIARALASDPTFM
                     LLDEPFAGVDPISVVDIQRIVSHLNERGIGVLVTDHNVRETLGICKRAYIVNEGAILA
                     EGKPRDLLADAATREIYLGEDFHL"
     gene            3963..>4154
                     /locus_tag="VFX38_07960"
     CDS             3963..>4154
                     /locus_tag="VFX38_07960"
                     /inference="COORDINATES: protein motif:HMM:NF012530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HEX5314819.1"
                     /translation="MNLSPRPVANIGQRLVLAPRQSESLQILALPATDLAALIAARLE
                     TNPLHEREEFLGADPQESDA"
BASE COUNT          642 a         1447 c         1395 g          670 t
ORIGIN      
        1 agcacgagga tcgaagcacg catggtccgg accagttcgt agggcgcgac gaagtcccgg
       61 atcggcgccg aatcgacttc gagttccatg tgctcgccga cggtgacttc gactcccatg
      121 cgccggagca ggctcacggt aatggtgaca tcctgcagat gcgggacatt gccgagggtg
      181 aggggcgtgt cggcgagaag cgaggccgcg agaatgggga gcgtcgcgtt ttttgcgccg
      241 gagatgcgga tttcgccctc gagccgcgcg ccgccttcga tgagaagctt gttcacgccc
      301 gctcgcgcgc ctcctcggga gtgagtgtct cgagggtgag cgcgtgaatc tctccgccca
      361 tgcgttcgcc gagcgccgcg tacacttcgc gatgccgggc aaggcgcgga tgtcctgcaa
      421 agcgtggcgt gacgatgcgg gcggaaaaat gcactccgtc ggccgtcgca acggcaacct
      481 ccgcttccga aaaggaaccg gtgagaagat tttcgagttc ggcgcggttc acgcggactt
      541 gccgggcgga caggaaacag gctttcgagt ttagcagagc gccgcgcgag cggggcgcga
      601 ctacaataac cggcagattt cccgacgcga ttgccatgcc cgccaccgct ccgcccgcgt
      661 ccgccgccca gttgattcgc tccgggcgcg aggtggtcgt gatcgaagcg gctgcggtcg
      721 cggcgctcgc cgctcgcctg aacgagacct tcgccgccgc ctgcggcctg ctgctcgcct
      781 gtcgcgggcg cgtggttacg accggcatgg gaaaatccgg gcatatcgcg cgcaaagtgg
      841 ccgccaccct cgcctccacg ggctcgcccg ccttctacgt tcaccccggc gaggccagcc
      901 acggggatct cggcatgatt accggcgaag acgtgttgat cgccgtctcc cattcgggtg
      961 aaacgccgga ggtcctggcc atcctttccc tgctcaagcg cctcggcgcc gggctgatcg
     1021 cgcttacggg caatcccgcc tcgaccctcg cccgcgcggc cgactgccat ctcgacgttg
     1081 ccgtctcgcg cgaagcctgc ccgctcaatc tcgccccgac cgcgagcacc agcgccgcgc
     1141 tcgccatggg cgatgcgctc gcactcgcgg tttcggccgc gcgcggcttt accgcggaag
     1201 acttcgcccg cgctcacccc ggcggcaacc tcggacgtcg cctgctgctg cgcgtcgccg
     1261 acctcatgca ccgcggcgag gccatccctc tcgtcggcga agccgcaacc ctgcgcgaag
     1321 ccctggtgga aatgagtgcc aagggcctcg gcatgaccct ggtgacggat tccgccggcc
     1381 agctcctcgg cctgttcacc gacggcgatc tgcgccgcag cctcgatcag ggcgccgggc
     1441 tcgcgagccc catcgccgat ctcatgaccc gcgaatgcac ggtggctgcg cccgagatgc
     1501 tcgccggtga ggttctgcgg ctgatggacc ggcgccgcat caacggcatg ccggtggtcg
     1561 ccgatggtcg cctcgtcggc gtgttcaata tgcacgatct cttgcaggcg ggagtcgtgt
     1621 gaaccgggag cgtctcgccg ccgtgcgcct gctcgccctc gacgtggacg gcgtgctcac
     1681 ggacggacgg gtgtggttct tcgacgacgg ccacgaggcc aaggccttct cgattcgcga
     1741 cggacacggc atcaaacgcc tcctcgccgc cggcgtcgca gtgataatta tcagcggccg
     1801 gcgctccgcg gcgagcgctc atcgcgcaca ggaactcggc atcacggcca tccacgaggg
     1861 cgtgagcgac aaggcgggcg tgctcgccga ggcggcggcc acggccggcg tggcgctcgc
     1921 cgactgcgcc ttcatgggcg atgacgagcc cgacatgccg gcactcgaag ccgccggcat
     1981 cgcactggcg cccgcggacg cgatccccgc ggtgcgcgcg ctcgccgact ggtgcgccac
     2041 gagcgcgggc gggcgcggcg ccgtgcgcga ggcctgcgag ctgctgctcg cggcgagaca
     2101 atccgcgtga ggaccttgct gcgccgggcc ttcgcggtcg cgctggtgct ggtggccggc
     2161 ttcacgagct ggttgatcat cgccaatcac gcctccgtcg cgccgccggc gcctgcgccg
     2221 gtctcggacc ggtccccgga ctacacgctc cgcgacgccg tggtcacgcg ttacgatccc
     2281 aagggcgcac ggcgttacgt gctgcgggcg caaagcatcg cccatcttcc cgcgagcggt
     2341 gcctcggtct tcggacagcc ccgcctcgat tatgtcgccg ggcccggcaa tgcgtggcgg
     2401 gtcaccgcgg agcgcggacg cctcggctcc gacaaccatc tgctgctgct tcggggaaag
     2461 gtgtacggcc ggcaactcca aggcgacaat ccgcttcgaa tcacaactgc ggcggcaatg
     2521 gtgcaactgg atcggcgcat cgtgcgctcg aggagtccgg tgcggctctg gcgcgaaggc
     2581 tttcaaaccg atggcatcgg gctcgacgcc gatctcacga gcggcgtcgt gaagctgttg
     2641 gcggaagttc gcagccgcta tgcgccgtaa gtcgcggccg gcgcgaaggc ttctcgcggc
     2701 gttcctcgcg gcggtcgcgg cggcgggcat gctcgccgcc gcgcaagcgc ccattctggt
     2761 gagcgccgag cgcagcacgg cgctcctgcc cgagggcgac cacgcacagg ttacgtatga
     2821 aggcgccgtg gtcatcacgc atggcgccgc ggtcattcat ggcgatcggg ccgtcctcta
     2881 catgaagaac cagcacctgg accgggcggt cgtgagcggc gcaccggcga ccttcatctg
     2941 gcagccgcag acgggagttc cggtccacgg cgaggccgcc atcattacct atcttgccgc
     3001 cgccgataca gtcgtcttcg cgggccgggt caaggtccat cgcgagtcgc agaccctctc
     3061 cgccgccgag gcgcgataca cattgaaaac cggcacgctt tcggcccgcg gcgggaccgg
     3121 aaccaatccc gagcgggttc atgtcgtcat tccgccggcg ccacccgcaa cggcgccgcg
     3181 gccgtgaagc gactggccgc ggagaatctg cacaagcgtt ttcgcggccg cgaggtggtc
     3241 aagggcctca gcctgcacgt ggaagcgggc gaagtggtcg gactgttagg ccccaatggc
     3301 gccggcaaga ccacctgctt ctacatgatt ctggggctca ttcgctgcga tgccggccgc
     3361 atcacgctcg acgagcgcga cctcaccgcg cttcccgtac accagcgggc ccgcgctggt
     3421 ctcggttatc tgccgcagga ggcgtccatc ttccgtcacc tgagcgcccg cgacaacgtg
     3481 ctcgcggtgc tcgaactcaa tcagacgaaa gtcaagcggc gcgatcgcgg gcaaagggcg
     3541 gatgaattgc tggaggcgct cggcgtgcag catcttgccg acacccgcgg cgacagtctt
     3601 tcggggggcg aacgccgccg cgtcgaaatc gcgcgcgcac tcgcctccga tccgaccttc
     3661 atgctgctcg acgagccgtt cgcgggcgtg gatccgatct cggtggtcga cattcagcgc
     3721 atcgtctcgc acctgaacga gcgcgggatc ggcgtgctcg tgaccgatca caacgtgcgg
     3781 gaaacgctgg gaatctgcaa gcgggcctac attgtcaacg aaggcgccat cctggccgaa
     3841 gggaagcccc gggatctgct ggccgatgcg gccacccgcg aaatctatct cggcgaggac
     3901 tttcatttgt aggcgtgtac catgagagac atgtcccggc gttcttcgcg attccccgcc
     3961 ccatgaacct gtcgccgcga ccggtcgcga atatcgggca acgtctcgtg ctggcgccgc
     4021 ggcagagcga atccctgcag atcctcgccc tgccggcgac ggatctggcc gcgctgatcg
     4081 cggcacggct ggagaccaat ccgctccacg agcgcgagga atttctcggc gcggacccgc
     4141 aggagtccga cgcc
//
LOCUS       DASUJG010000372         2060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1818543, whole genome shotgun sequence.
ACCESSION   DASUJG010000372 DASUJG010000000
VERSION     DASUJG010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1818543"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VFX38_07965"
     CDS             complement(<1..457)
                     /locus_tag="VFX38_07965"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HEX5314820.1"
                     /translation="MSTDTLLRQAVKGALALGSAGTMVGAGAALAQTAAPAAATATPL
                     SNIVVTGSHIPRTSIATAQPIVTINQQQIAATGFTTTDQLLQNIVSTGASLNVQFNNG
                     GNGSATINLHDLGAQRVLVLVNGQRWISSLGGTVDLTTIPLAAVDHVDVL"
     gene            913..>2060
                     /locus_tag="VFX38_07970"
     CDS             913..>2060
                     /locus_tag="VFX38_07970"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5314821.1"
                     /translation="MNATVPARLPSAAMRRMRSAAAAQQRRDYASAEALYRSLTREYP
                     DFADAWHFYGLLLHQRGEGRQALAMLERAERLTSDNFPFLVNHGRVLLEIGEYRRAYA
                     QLERARRHPAADPSALKLLIHAALKLGREAEMLPLLDEQLTGALARNAGDWQLWILLG
                     ECRERSGDRPGALAAFAEAGVFAPAGEVTPQLRQGSAALSAGQLEPARVAFEQALRRL
                     PDSPEALLGLANVAAREGDFGACQRLARQALARNPRLYIGWLLRVDSLGNGSAEALAA
                     DMETAAKRAGEDPEAFPLHFARGQLREKLGDYETAFAAYELGNRQRGRSYPYQAAAQT
                     AYSRDVIESLDEAFVARAPAVGVPGAGPIFVIGMPRSGTTLVETILAS"
BASE COUNT          344 a          718 c          681 g          317 t
ORIGIN      
        1 gcagcacgtc cacgtggtcc accgcggcga gcggaatggt ggtcaagtcc accgtgccgc
       61 cgaggctcga aatccagcgc tgcccgttca cgagcaccag cacgcgctgg gcgcccagat
      121 cgtgcaggtt gatggtggcc gagccgttgc cgccgttgtt gaactggacg ttcagcgagg
      181 cgccggtgga gacgatgttc tgcagcaact gatcggtcgt cgtgaaaccg gtcgcggcga
      241 tctgctgctg gttgatggtg acgatcggct gagcggtcgc gatcgaggtg cgcggaatgt
      301 gcgaacccgt gaccacgatg ttgctcagcg gggtggccgt ggcggccgcc ggcgccgcag
      361 tctgggccag cgccgcgccc gccccgacca tggtgccggc cgagccgagc gcgagcgcgc
      421 ccttcacggc ctgccgaagc aatgtatcgg tactcatgac aaaaatacct cccagctaag
      481 agggttgaat ccacaatcgg cggcccaatt gccgcctcgc cgccgcgagc cctgcgcaga
      541 cacacacgcc cactcgcgga gggtgaggga atcctagcac caccggaatc gttttgcaag
      601 cctttgttgc agagcggaga cagacgagac ccgccgggga cgggcatgac agacaaatcg
      661 acgggcaacc gcggtcgctt gttgcatcca cacaacacaa gatcgcagca ttgcttcggg
      721 gagatcgccc cggccgtcac accctccggg agagcctcgc ggccttggcc cgaaagccat
      781 tgtcggccgc agcggccgat gcctcgcccg agcgccgcga caaaggtacg ggctcgtgcc
      841 ggcccgcgac attggcgctc gatcggcgac ccgggagccc cgtgcgcttc gtctcctgca
      901 ataatgcctc ccatgaacgc aaccgtaccc gcccggctcc cgtctgccgc gatgcgacgc
      961 atgcgcagcg ccgccgcggc ccagcagcgg cgcgactacg cctcggcgga ggccttgtac
     1021 cgcagcctta cgcgagagta tccggacttc gccgacgcct ggcatttcta cgggttgctg
     1081 ctgcaccagc gcggcgaggg ccggcaggca ttggcgatgc tggagcgggc ggaacggctg
     1141 acctccgaca atttcccgtt tctcgtcaat cacggtcggg tcctgctcga gatcggcgaa
     1201 taccgccgcg cctatgcgca gctcgagcgt gcgcgccgcc atccggccgc ggatccttcc
     1261 gccctgaaat tgttgattca tgcggcactg aagctcgggc gcgaggcgga aatgcttcca
     1321 cttctcgacg aacagctcac cggagcgctc gcgcgcaacg ccggtgactg gcaactttgg
     1381 attctgctgg gagagtgccg cgagcgcagc ggcgatcgcc ccggcgcact cgccgccttt
     1441 gccgaagccg gggtttttgc cccggcgggc gaggtcacgc cccagcttcg ccagggatcg
     1501 gcggccctga gcgcgggaca actcgaaccc gcgcgcgtcg ccttcgaaca ggcgctgcgc
     1561 cgcttgcccg attctcccga ggccctgctc ggtctggcca acgttgccgc acgggaaggc
     1621 gacttcggag cctgccagcg gctcgcgcgc caggcgcttg cgcgcaatcc gcgcctttac
     1681 atcggctggc ttctgcgcgt cgacagcctc gggaacggct ccgcagaggc gctcgccgca
     1741 gacatggaaa cggcagccaa acgcgcgggc gaagatccgg aggccttccc gctgcacttc
     1801 gcgcgcggcc aactacggga aaaactcggc gactacgaaa cggccttcgc cgcctacgaa
     1861 cttggcaatc gccagcgggg gcgaagctat ccctatcagg cggcggcgca gacggcctac
     1921 agccgcgatg tgatcgaatc cctggacgaa gccttcgtcg cccgggcgcc ggcggtcggc
     1981 gtgccgggcg cgggaccgat cttcgtgatc ggcatgccgc gctcgggcac gaccctcgtc
     2041 gaaaccattc tcgcgagcca
//
LOCUS       DASUJG010000373         2052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1452663, whole genome shotgun sequence.
ACCESSION   DASUJG010000373 DASUJG010000000
VERSION     DASUJG010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1452663"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /gene="epmA"
                     /locus_tag="VFX38_07975"
     CDS             <1..1082
                     /gene="epmA"
                     /locus_tag="VFX38_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HEX5314822.1"
                     /translation="WWDRERYARRHGNLLARGRMAAAIRAYFSAEGFAEVETPALQVS
                     PGLEVHLRAFATVLNEPFGQDSRTLHLHTSPEFAMKKLLAAGEERIFQFARVYRNGER
                     SATHHPEFTMLEWYRADAGWRETAEDCRRFVVAALDALAPGCTLTREGRAADPRRSWE
                     YLSVSDAFARFAGIDLPACLAPDPKNPDPTLLREAAARAGHALPEKGEWDDLFFHLFL
                     AHVEPNVGSPAPTVLHGWPAAMAALAKISEDDPRFAERFEVYAAGLELANGFTELTDA
                     REQRARFEADQARKERLYGERYPIDEDFLAALEAMPPAAGVALGFDRLVMLATGAESI
                     EDVLWAPVAGGMARDAARAPVRSPY"
     gene            complement(1042..1695)
                     /gene="thiE"
                     /locus_tag="VFX38_07980"
     CDS             complement(1042..1695)
                     /gene="thiE"
                     /locus_tag="VFX38_07980"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX5314823.1"
                     /translation="MDAPEERCRLYLITPPRLDPVAFADRLSAALDGGDVASVQLRLK
                     DASDDEVRRAIDALRPVAQSRNVAFILNDRPDLASASGCDGVHVGKDDPDVAEARRIV
                     GADAIVGVSAYDSRHRAMEGADDGADYVAFGAFFPTTTKIAEGHPEPEVLAWWSELMT
                     VPCVAIGGIKVENGAVLVEAGADFLAVSSGVWDYPDGPAAAVKAFNTVIEQALARRP"
     gene            complement(1708..>2052)
                     /locus_tag="VFX38_07985"
     CDS             complement(1708..>2052)
                     /locus_tag="VFX38_07985"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5314824.1"
                     /translation="ELLGLRPLKKPDSLGPAGQHYCLSETPYAELHLGVARNAEGQSN
                     KAMRNHLGFQVGDLAAARKRFEAAGVRIEDAEPAYSKERGYHQDRFFVFDPGGNRLEI
                     LEPRRSYDSAQH"
BASE COUNT          305 a          684 c          722 g          341 t
ORIGIN      
        1 cctggtggga ccgcgagcgc tacgctcgcc gtcacggcaa tcttctcgcc cgtgggcgga
       61 tggcggccgc gatccgtgcg tatttttcgg cggaaggttt cgccgaggtg gagacgccgg
      121 cgcttcaggt ctcgcccggt ctggaggtgc acctgcgcgc cttcgcaacc gttcttaacg
      181 agccgttcgg gcaggactcg cgcacgctcc atctccatac gtcgcccgaa ttcgcaatga
      241 agaagctgct cgcggcgggc gaggagagaa tttttcagtt cgcacgggtt tatcgcaacg
      301 gcgagcgctc ggcgacccat catcccgagt tcacgatgct ggaatggtat cgggcggatg
      361 cgggctggcg agagaccgcg gaagattgcc gccggttcgt cgtcgccgcg ctcgatgcgc
      421 ttgcgcccgg ctgcacgctg acccgggagg gacgcgccgc cgatccgcgg cggtcgtggg
      481 aatatctttc ggtgagcgat gcgtttgcgc ggttcgcggg gatcgacctt cccgcctgcc
      541 ttgctcccga tccgaagaac cccgacccca cgctcctgcg ggaggcggcc gcacgcgcgg
      601 gccacgcgct ccccgaaaag ggcgagtggg acgacctctt ctttcatctc tttctagccc
      661 acgtcgagcc gaacgtcggg agcccggcgc cgaccgtgct tcacggctgg cccgcggcga
      721 tggcggcgct cgcgaaaatc tccgaggacg atccgcgttt cgccgagcgc ttcgaggtct
      781 acgccgccgg cctcgagctc gccaacggct ttaccgagtt gaccgacgcg cgcgagcagc
      841 gcgcccgctt cgaggcggat caggcgagga aggagcggct ttacggcgag cgctatccga
      901 tcgacgagga ttttcttgcg gctctggagg caatgccgcc cgcggcaggg gtcgcgctcg
      961 gcttcgatcg cctggtcatg ctcgcgaccg gcgccgaatc gatcgaggac gtcctgtggg
     1021 cgccggtggc gggtggtatg gctagggacg ccgcgcgagc gcctgttcga tcaccgtatt
     1081 gaacgccttc accgccgccg ccgggccgtc cgggtagtcc caaacgcccg aggagacggc
     1141 gaggaagtcg gcgccggctt ccaccagcac ggcgccgttc tcgaccttga tgccgccgat
     1201 ggcgacgcac ggcaccgtca taagctccga ccaccaagcg agaacctccg gctcggggtg
     1261 cccttcggcg atcttggtgg tggtcgggaa gaacgcgccg aacgcgacat agtcggcgcc
     1321 gtcgtcggcc ccttccatcg cgcggtggcg cgaatcgtag gccgagacgc cgacgatggc
     1381 gtccgcgccg acgatccggc gggcctccgc gacgtctggg tcgtccttac cgacatgaac
     1441 gccgtcgcag ccggaggcgg acgcgagatc ggggcggtcg ttcaggatga acgcgacatt
     1501 gcgcgattgg gcgacggggc ggagcgcgtc gatcgcgcgg cgcacctcgt cgtcgcttgc
     1561 gtccttgagg cgcaattgca cggaggcgac gtcgcccccg tcgagcgccg ccgacagccg
     1621 gtcggcgaac gccacgggat cgagcctcgg cggcgttatg aggtagagcc gacagcgctc
     1681 ttcgggggca tccacgcgtg caggggccta gtgctgcgcg ctgtcgtaag aacggcgcgg
     1741 ctcgagaatt tccagccggt tgccgcccgg atcgaagacg aagaagcgat cctggtggta
     1801 cccgcgctcc ttcgagtacg cgggttcggc gtcctcgatg cgcaccccgg ccgcctcgaa
     1861 ccgcttgcgc gccgccgcca gatcgccgac ctgaaagccg aggtggttgc gcatcgcctt
     1921 gttcgactgg ccctcggcgt tgcgcgcgac gccgagatgc aactcggcgt atggggtttc
     1981 ggagaggcag taatgctgcc cggcggggcc gaggctgtcg ggcttcttca gaggcctaag
     2041 cccgagcaat tc
//
LOCUS       DASUJG010000374         6479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1086951, whole genome shotgun sequence.
ACCESSION   DASUJG010000374 DASUJG010000000
VERSION     DASUJG010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6479
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086951"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VFX38_07990"
     CDS             complement(<1..686)
                     /locus_tag="VFX38_07990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX5314825.1"
                     /translation="MKAEGAAQLHRGPLTVAVMLATLMQVLDMTIANVALPHMQGSFA
                     VSLDQIKWVLTSYIVASAVATPLMGWLAERYGRRNVLLAAIGAFVVFSFMVGSSVAIS
                     EIVIARILQGVAGAAFSPLAQAILLDAYPRERHGTAMAILGVGILVGPILGPIVGGFL
                     TQYYSWRWCFFINVPLGALALLLVWNFVPRRLVRRKLSFDWLGFVMLGLGIGALQFML
                     DRGSTKNWFSS"
     gene            complement(683..1753)
                     /locus_tag="VFX38_07995"
     CDS             complement(683..1753)
                     /locus_tag="VFX38_07995"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEX5314826.1"
                     /translation="MAEEKESASGERSRPWLRRLRFALMILGPAILIAVGLWYYIAHK
                     GYVSTDDAFVQANAVRVSPQVAGRIVAVPVHTNERVKKGSVLLRLDPAPYDAAWRAAE
                     AKVAATADQVRATKAQYQALDAQVAGAQAQVAFLEREVHRQGPLAKKNVVTNAKLDEL
                     TTRLAQARQQVAALAAQRDQALAALGGDPDQPLAKNAGYRAALAALAQAKLNRSYTVV
                     RAPADGIVGPVDVRPGDVLPAGGAAFPLVETAEVWIKANFKETALTHMHPGDPATVTV
                     DSYPGRSWKAHVESISPASGEVYALLPPQNASGNWVKVVQRIPVRLAIEREANEPMLR
                     AGMSAEVSVYVGGKSKKRPPSQ"
     gene            1908..3224
                     /locus_tag="VFX38_08000"
     CDS             1908..3224
                     /locus_tag="VFX38_08000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEX5314827.1"
                     /translation="MRRLLPAIALPVLLLASSFAGAETLRQALAYAWLHNPDLLAARA
                     TQTAAQSDIRAAKGAWYPQLALTGGIARDHESGEITFFPEPTTFDADLDQNRIALRLD
                     QTLYQGGRIAADVSAARDEASAAHAQTRADESSVLLNVVRTYLGVVEAQAVLAVQERN
                     IAVLHRQWEAARQSLAHGEGTRTDVAQAESRLQAGIAARIRAAANLAEMRAEYRAVVG
                     HEPLATSLPTEAPALPASLQQAEALASENDYVAAARFTAEAAAAQADAAHGALYPSLG
                     LYAEINRQNNPQFGFSTLTDREIGVDVSIPIWQGGSLRARSSAARARADSASFEARSA
                     SQQAEAQAASAWQDHLAAHSAIAATQAQLDAARIAFRGVEAEHRQGERTLLDVLNAEQ
                     EVRNGEVALVQTQRDNLLADYALLAATGSLSAAALHLAPPAAGSAP"
     gene            3221..3841
                     /locus_tag="VFX38_08005"
     CDS             3221..3841
                     /locus_tag="VFX38_08005"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5314828.1"
                     /translation="MNAASEEGLSAKQTDILGAAWQVFLRESYAMASMESIAQEAGVS
                     KQTVYNHFGSKEDLFRAIIERRCERLSAAFGEEFRYHGDEPGRLLTVFGERFLEIMLS
                     KETMELHRLLQAESRRHPRLARIFYEYGADRTARQLADYLAEQDTKGRLRIRDARLAA
                     EQFITMLPGHLRLRHLLGTAPPPTAAERSRYIASAVRLFLDGVRPR"
     gene            complement(3991..4761)
                     /locus_tag="VFX38_08010"
     CDS             complement(3991..4761)
                     /locus_tag="VFX38_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEX5314829.1"
                     /translation="MNEIRPRSSNPALRTIARRGLPQAAAGEAAMTVGGTVNRTIVMI
                     ALVVIAAAWVWSRYALALPDTEAAAKAVSGYLIAGVIGGLVLAIATVFKPTWARVTAP
                     AYALLEGCFIGGISALLNNRYPGIVLEAVGLTFAVMIGMLLLYKTRIIKVTPALQRGI
                     IIATFGVLLFYGVSWLLTLFHVDTSMLFGNTKLSIIISLVIVGIAAFNLVLDFHFIDQ
                     GAAAGLPRYMEWYGAFALTVTLVWLYLEILRLLAATRR"
     gene            4843..5772
                     /locus_tag="VFX38_08015"
     CDS             4843..5772
                     /locus_tag="VFX38_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEX5314830.1"
                     /translation="MPIEARTRAPARPLPAPAPPLTIAISSRALFDLEASHRIFVEQG
                     LAAYARYQIAHEDELLQPGVAFALVEKLLRLNDAEHRRVNVILLSHNSADTGLRIFRA
                     IEHYGLDITQAAFTNGRSPYHYVSPFGADLFLSADPGDVARALAEDMAAATIIPKRVT
                     PAAGAQLRIAFDGDAVLFSDEAERVFREQGIEAFGESERRAAREPLAGGPFKNFLAAL
                     HRIQSDYPIEGSPIRTALVTARSAPAHERAIRTLREWNIRIDEALFLGGLDKAPFLKT
                     FGADIFFDDQRAHCEVASDLVATGHVPHGIANE"
     gene            5769..>6479
                     /locus_tag="VFX38_08020"
     CDS             5769..>6479
                     /locus_tag="VFX38_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX5314831.1"
                     /translation="MSERRNWREERQSAWLYRILAEREAAAPKRAGLFRDLAAAADRQ
                     AETWERRMQAIGETPPRFRPTLRARIVAVLIRRIPPAQLQSVLAAMKLRGLSIYALAA
                     APATHPQPARAGAPESFHRRLERGGSLRAAIFGVNDGLVSNTSLLLGLVGAGMDRGVI
                     LLAGVAGLVAGAASMAAGEYVSMRAQREMFEYQIGLEREELAVYPEEEAAELALIYTA
                     RGMEPAAARNLADDTIARP"
BASE COUNT         1134 a         2267 c         2074 g         1004 t
ORIGIN      
        1 gagctgaacc agttcttggt gctgccgcga tcgagcatga actggagcgc gccgatgcct
       61 aggccgagca tcacgaaacc gagccagtcg aagctcagct tgcgccgcac gagccggcgg
      121 ggtacgaagt tccacaccag cagcagcgcc agcgcgccga gcggcacgtt gatgaagaag
      181 caccagcgcc aactgtagta ctgggtgaga aagccgccga cgatgggccc gagaatcggt
      241 ccgacaagga tgccgactcc gagaatggcc atcgccgtgc cgtgacgttc gcgcgggtag
      301 gcgtcgagca ggatcgcctg cgcgagcggc gaaaatgctg cgccggcgac gccctgcaaa
      361 atgcgggcga tcacgatctc gctgatcgcg accgaagagc cgaccatgaa cgagaagacg
      421 acgaaggcgc cgatcgcggc gagcagcacg ttgcgccgcc cgtaacgctc cgcgagccag
      481 cccatgagcg gcgtcgccac cgccgaggcg acgatgtagg aggtgagcac ccacttgatc
      541 tgatcgaggc tgaccgcgaa gcttccctgc atgtgcggca gggcgacgtt cgcaatcgtc
      601 atgtcgagca cctgcatcag cgtcgcgagc atcaccgcga cggtgagcgg cccgcggtga
      661 agctgcgcgg cgccctcggc cttcattgcg acggcggtcg cttcttcgac ttccctccga
      721 cgtacacgct cacttcggcg ctcatgccgg cgcgcagcat cggctcgttc gcctcgcgtt
      781 cgatcgcgag acgcacggga atgcgctgca ccactttcac ccagttgccg ctcgcgtttt
      841 ggggcggaag gagcgcatag acttcccccg aggccgggct gatgctctcg acgtgcgcct
      901 tccagctacg ccccggataa ctgtccacgg tgacggtggc cggatcgccc ggatgcatgt
      961 gcgtcaaggc cgtttccttg aaatttgcct tgatccagac ctcggccgtt tcgaccagcg
     1021 gaaaggccgc gccccccgcg ggaagcacgt cgcccgggcg cacgtccacc ggccccacga
     1081 tgccgtcggc gggagcgcgg accaccgtgt aggagcggtt gagcttcgcc tgcgcaagcg
     1141 cggcaagtgc ggcgcgatag cccgcatttt tcgcgagcgg ctgatcgggg tcaccgccga
     1201 gcgcggcgag cgcctggtct cgctgagccg cgagcgccgc gacctgctgc cgcgcctggg
     1261 cgagcctggt cgtcagttca tccagtttcg cgttcgtcac gacgttcttc ttcgcgagcg
     1321 gcccctggcg atgcacctcg cgctcgagaa aggcgacctg cgcctgcgcg cccgcgacct
     1381 gggcatcgag cgcctgatac tgcgctttgg ttgcccgcac ctggtcggcg gtcgccgcga
     1441 ccttcgcttc ggcggcgcgc caggcggcat cgtagggcgc aggatccagc cgcagaagca
     1501 cgctgccctt tttcacccgc tcgttcgtgt gcacgggcac ggcgacgatg cgcccggcca
     1561 cctgcggact cacgcgcacg gcgttcgcct gcacgaaggc gtcgtcggtg ctgacgtagc
     1621 ccttgtgggc gatgtagtac cacagcccga ccgcgatcag aatcgccggg cccaggatca
     1681 tgagcgcgaa acggaggcgc cgcagccagg gacgggagcg ctcgccggag gccgattctt
     1741 tttcttccgc catgcggccc gccacttgac tggactggac ggtctagtct aaacctccgt
     1801 ccgtgttggg tcaatgtcgc aggcaattac ctgatgagac gccgatccct tgtgccgaat
     1861 gccggcagcg catagaatgg actggacggt ccagtccagg aatcactttg cgcaggctgc
     1921 tgccggcgat cgcgctgccg gttcttcttc tcgcaagctc cttcgccgga gcggaaacgc
     1981 tgcgccaggc gctcgcctac gcctggctgc acaatccgga tctgctcgcg gcccgtgcaa
     2041 cgcaaaccgc cgcacagagc gacatacgcg cggcgaaggg cgcgtggtat ccacagctcg
     2101 cgctcacggg cggcatcgct cgcgatcacg agagcggcga aatcaccttt ttccccgaac
     2161 cgacgacttt cgatgcggac ctcgatcaga atcgcatcgc cctgcggctg gaccagacgc
     2221 tctatcaggg cgggcggatc gcggccgacg tttccgcggc ccgcgacgag gcttcggcgg
     2281 cgcacgcgca aacccgcgcc gacgaaagct ccgttctgct gaatgtggtg cgcacctatc
     2341 tcggagtggt cgaggcgcag gcggtgctcg ccgtgcagga acgcaacatc gccgtgttgc
     2401 atcggcagtg ggaggccgca cggcaatcgc tcgcgcacgg cgaaggaacg cgcacggacg
     2461 tcgctcaggc cgaatcgcgt ctgcaggccg gcatcgcggc gcggattcgc gccgcggcga
     2521 atctggcgga aatgcgggcc gaataccgcg ccgtcgtcgg ccacgagccg ctcgcgactt
     2581 ccctgccgac ggaagcgccg gcccttcccg ctagcctcca acaggccgaa gcgctcgcga
     2641 gcgagaacga ttacgtcgct gccgcgcgat tcaccgccga ggccgccgcc gcccaggccg
     2701 atgccgcgca tggcgcactc tatccctcgc tcgggctcta cgccgagatc aatcgccaga
     2761 acaatccgca gttcggattc tcgaccctca cggatcggga gatcggcgtc gatgtcagca
     2821 ttcccatctg gcaaggcggg tcgctgcgcg cgcgcagttc cgccgcccgg gcgcgtgccg
     2881 attccgcatc gttcgaggct cgctccgcct cgcagcaggc cgaggcacag gcggcgagtg
     2941 cctggcagga tcaccttgcc gcgcattcgg cgatcgcggc cacgcaggcg caactcgatg
     3001 ccgcgcgcat cgccttccgc ggtgtcgagg ccgaacaccg tcagggcgaa cgcaccctgc
     3061 tcgacgtgct caacgcggag caggaagtgc gcaacggcga agtcgctctg gtgcagacgc
     3121 aacgcgacaa tctgctcgcc gactacgccc tcctcgcagc gaccggaagc ctttccgcgg
     3181 cggcgctgca cttggcaccg cccgcagcag gtagcgcgcc gtgaacgccg cctccgagga
     3241 aggcctgagc gccaagcaga ccgacatcct cggcgccgcc tggcaggtgt tcctgcgcga
     3301 aagctacgcc atggcgagca tggaatcgat cgcgcaggag gccggcgttt ccaagcagac
     3361 cgtctacaac cacttcggca gcaaggagga tctcttccgc gcgatcatcg agcgccgctg
     3421 cgaacggctg agcgccgcct tcggcgagga attccgctat cacggcgacg agccggggcg
     3481 ccttctcacg gtcttcggag aacgctttct cgagatcatg ctctcgaagg agaccatgga
     3541 actgcatcgc ctgctgcagg cggagagccg ccgtcatccg cgtctcgcga gaatcttcta
     3601 cgaatacggc gccgaccgca ctgcgaggca gctcgccgat tatctcgcgg aacaggacac
     3661 gaaggggcgg ctgcgcatcc gcgacgcccg cctcgcggcc gaacagttca tcacgatgct
     3721 gccggggcat ctgcgcctgc gtcaccttct cggcaccgca ccacctccca ccgcggcgga
     3781 gcgttcgcgc tacatcgcct cggccgtgcg tctctttctc gacggcgtgc gtccccggta
     3841 acgcaagctc ccacgtgaat tcgcctgctg aaaagcgtag cgagcgaagg cagattgggc
     3901 gcggccggag cgcagaaacc gcagcgtacc aacaagtacg tgaggatttc gagcaccgcc
     3961 cgcgctcaag atgccgagcg cagcagcttt tcagcgtcgg gttgcggcga ggagccgcag
     4021 aatctccaga tagagccaca ccagggtcac ggtgagcgcg aaggcgccgt accactccat
     4081 gtagcgcggc agacccgccg cagcgccctg atcgatgaaa tggaagtcga gcaccagatt
     4141 gaaggccgcg atgccgacga tcacgaggct gatgatgatg gaaagtttcg tgttgccgaa
     4201 aagcatcgag gtgtccacgt gaaagagtgt caacaaccac gacacaccat agaaaagaag
     4261 cacgccgaag gtcgcgatga tgatgccgcg ctgcagcgcc ggcgtcacct tgatgatccg
     4321 ggtcttgtag agcaggagca tgccgatcat gacggcgaag gtgagaccca ccgcctcgag
     4381 cacgatcccg ggatagcggt tgttgaggag cgcggagatt ccgccgataa agcagccttc
     4441 gagcagcgcg taggcgggtg ccgtgactcg cgcccaggtg ggcttgaaga ccgtcgcgat
     4501 cgcgagcacg agcccgccga tcaccccggc gatcaggtaa ccgctgaccg ccttcgccgc
     4561 cgcttccgtg tcgggcagcg cgagggcgta gcggctccac acccaggccg cggcgatgac
     4621 gacgagcgcg atcatcacga tggtgcgatt caccgtgccg ccgaccgtca ttgccgcctc
     4681 gccggcggcc gcctgcggca ggccccggcg ggcaatcgta cgcagggcgg ggttgctgct
     4741 cctcgggcgg atttcgttca tgcttgctcg cagaggggaa atcggtgcac attatgaccg
     4801 atcgccgcgc ccctggttcg ccgcctgtgc gaaaatttgc cgatgccgat tgaagcccgg
     4861 acacgagccc ccgcgcggcc gctccccgcg ccggctccgc cgctcacgat cgcgatttcc
     4921 tcgcgcgcgc tgttcgatct cgaggcgagc catcgcatct tcgtcgagca gggcctcgcc
     4981 gcctacgccc gctaccagat tgcgcacgaa gacgaactcc tgcaaccggg cgtcgccttc
     5041 gcgctggtgg aaaaactgct gcgtctgaac gacgccgaac accgacgggt caacgtgatc
     5101 ctgctctcgc acaacagcgc cgacacggga ctgcgcattt ttcgcgcgat cgaacactac
     5161 ggactcgaca tcacccaggc ggccttcacc aacggccgct cgccctatca ctacgtgagt
     5221 cccttcggcg cagacctttt cctttccgcc gatcccggcg acgtggcgcg ggcgctcgcc
     5281 gaagacatgg ccgcggcgac gatcatcccc aaacgcgtca cgcccgccgc gggagcgcag
     5341 cttcgcatcg ccttcgacgg cgacgccgtg cttttttccg acgaggccga gagggtgttt
     5401 cgcgagcagg gcatcgaggc atttggcgaa agcgaacgcc gcgccgcccg cgaaccgctc
     5461 gccggcggac ccttcaagaa ctttctcgcg gcgctgcatc gcatccagag cgactacccg
     5521 atcgaaggct cgcccatccg caccgcgctc gtcacggcgc gttccgctcc ggcgcacgag
     5581 cgcgcgatcc gcaccctgcg cgaatggaac atccgcatcg acgaggcgct ctttctcggc
     5641 ggactcgaca aggcgccgtt tctcaagacc ttcggcgccg acatcttctt cgacgatcag
     5701 cgcgcacact gcgaggttgc ttccgatctc gtcgcgaccg gccacgttcc gcacggcatc
     5761 gccaacgagt gagcgaacgc cgcaactggc gcgaagaacg gcaatcggcc tggctgtacc
     5821 gcatcctcgc cgaacgggaa gcggccgcac ccaaacgcgc cgggcttttt cgcgatctcg
     5881 ccgccgccgc cgaccgtcag gccgaaacct gggaacgacg catgcaggcg ataggcgaaa
     5941 cgccgccgcg ctttcgcccg acgctccgcg cacgaatcgt cgccgtactc atccgccgca
     6001 ttccgcccgc gcaactccag agcgtgctgg ccgcgatgaa actgcggggg ctgtcgatct
     6061 atgcgctcgc cgcggccccg gccacgcatc cgcaaccggc gcgggccggc gcgcccgaga
     6121 gttttcatcg tcgcctcgaa cgcggcggaa gcctccgcgc cgcgatcttc ggcgtgaacg
     6181 acgggcttgt ctccaacacc agtcttctgc tcggactcgt cggcgccgga atggatcgcg
     6241 gcgtgatcct gcttgccggg gtcgccggcc tcgtcgcggg cgcggcctcg atggcggccg
     6301 gcgaatacgt gtcgatgcgt gcgcaacgcg aaatgttcga ataccagatc gggctcgaac
     6361 gcgaagaact cgccgtgtac cccgaggaag aagccgccga actcgcgctc atctatacgg
     6421 cccgcggcat ggaaccggcc gcagcccgaa acctcgccga cgataccatc gcgcgaccc
//
LOCUS       DASUJG010000375         3041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1087012, whole genome shotgun sequence.
ACCESSION   DASUJG010000375 DASUJG010000000
VERSION     DASUJG010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087012"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VFX38_08025"
     CDS             <1..744
                     /locus_tag="VFX38_08025"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5314832.1"
                     /translation="HQDRVLHEDPAQKSWWWALRYVLSTRTNVILIIASALGYFYFTG
                     IRTFAVVFLRARFGLGQSAASSLLVVIGLGSIVGVLLAGRLADRLIQKGHLPARVTVG
                     AGAFLFAALLFLPGLLVPAFAAAAALFFFASMGLGAANPPLDAARLDIMHSRLWGRAE
                     SIRTALRFTLEAIAPLLFGYVSTFFGGGGATFAGRNTAQGGAGLDYTFLIMLVTLIAA
                     GIIMLRARKPYPRDVATAIASEHESGSGT"
     gene            1140..2270
                     /locus_tag="VFX38_08030"
     CDS             1140..2270
                     /locus_tag="VFX38_08030"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEX5314833.1"
                     /translation="MPGSITRGIVFFILSATSLMSCASAFAAAQGFGWDLSYKQDFLR
                     DVAGGKERGGGAPGMLEADGAWRWGSSEFKIGLLGTFGGSLYKRVGALQPPSDLKAYN
                     TLKLYKLWYGHRFGQTGLALRLGVQDYSALFNTLAAAQIFMNTSFGVAPTISQVHPSI
                     YPVTALGAVLHWQAHHVYALGGIYDGVPGLPGHPAGTHIELRSGDGTFSNVEAGIIGK
                     GYKLGVGGWYRTTDFKDMLGEPYDRNHGFYIVGSKMLFGGGGKPTLDVFVQLGSAEED
                     RNEMGRYIGAGVELQHFISGRPNDALGLGMARVLPSDLFQRATPGVTRPETVFEATYQ
                     AAISKSLVIHPDVQYITNPGAVPDYDNAWVVGFRLQYQMQGG"
     gene            2400..2657
                     /locus_tag="VFX38_08035"
     CDS             2400..2657
                     /locus_tag="VFX38_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HEX5314834.1"
                     /translation="MPGKSPGPQVKDKDRYEALRREGESKEKSARIANSPRSETARKG
                     GKSSAYEDWNKDDLEKRAREIGIEGRSRMSKNELINALRNH"
     gene            complement(2708..>3041)
                     /locus_tag="VFX38_08040"
     CDS             complement(2708..>3041)
                     /locus_tag="VFX38_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014059338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314835.1"
                     /translation="QHYAFLVSEIEFDEIFARIRERGLRYWADPRRKRGGEINSRDDG
                     RGCYFDDPSGHRLEIITRPYGSGGTTASSPHPLIAPRLTPHGQGASTHPQERRGGCEL
                     ADEEEPEG"
BASE COUNT          560 a          985 c          932 g          564 t
ORIGIN      
        1 caccaggatc gggtgctgca cgaggatccg gcgcaaaaat catggtggtg ggcgctgcgc
       61 tacgtgctct cgacgcgcac caacgtgatc ctgattatcg cctcggcgct cggctacttt
      121 tatttcaccg gaattcgcac ctttgcggtg gtctttctgc gggcgcgttt cggcctcggc
      181 caatccgccg ccagttcgct gctggtggtg atcgggctcg gctccatcgt cggcgttctc
      241 cttgcgggtc gcctggccga tcgactcatt caaaaaggcc atctccccgc gcgggtcacc
      301 gtcggcgcag gcgcgtttct cttcgccgcc ttgctgtttc ttccggggct tctcgtaccc
      361 gcctttgccg ccgccgcggc tttgttcttt tttgcctcga tgggtctcgg cgcggccaat
      421 ccgccgctgg acgcagcgcg cctggacatc atgcactcgc gtctgtgggg gcgggccgaa
      481 agcatacgca ccgcgctgcg cttcacactc gaagccatcg cgccgctgct tttcgggtac
      541 gtctcgacgt ttttcggcgg cggcggtgcg accttcgccg gacgcaatac cgcacagggc
      601 ggggccggcc tcgactatac ctttctgatc atgctggtca ccttgattgc ggcggggatc
      661 atcatgctgc gggcgcgcaa accctatccg cgcgatgtcg ccaccgcaat cgcgtcggaa
      721 catgagagcg gctccggcac ctgagcgccc ggcgacagga gaaagaagcc gcgatgcgac
      781 ccctttcctt tggcggacgc ggcagtaaag gcaaatagca gataaagcga aaagcggacc
      841 tggagcagac atcggcgaag cgcaagggct tcgtatcagc gcggagtatg cgtttctcgc
      901 gaccatgatc gaggcgacgc gcccgactcc tctctatgcg cgctgccaac gcgggttcgg
      961 gctcccaagt gatgggcgcc gccgcttcct gttcgcaacc atcgccgccc ctgacaatgt
     1021 gggctcgcgc acggaaaatc cttggattcc aacagcataa tcggggcaat ccatcaacgc
     1081 cgcgacagga aaaccgcaac cagaccgtca cggcttgcac tcgcgaggtg cgtcgcacaa
     1141 tgccagggtc gatcacacgc ggcatcgtct tcttcatcct gtcggcaact tcactcatga
     1201 gctgtgcttc cgcattcgcg gccgcacaag gctttggctg ggatctcagc tacaagcagg
     1261 attttctgcg cgacgtggcc ggagggaagg agcgcggcgg cggcgccccc gggatgctcg
     1321 aagccgatgg cgcctggcgt tggggcagca gcgagttcaa gatcggtctc ctcggcacat
     1381 tcggcggctc gctttacaag cgtgtcggcg cgctgcagcc gccctccgac ctcaaggcgt
     1441 acaacacgct caagctctac aaactgtggt acggccatcg ctttggccaa accggccttg
     1501 cgctgcgtct cggcgtgcag gactacagcg ctctgttcaa tacactcgcg gcagcccaga
     1561 tcttcatgaa cacctccttc ggcgtagccc cgacgatctc gcaggtgcat ccgagcatct
     1621 atccggtcac tgcgctcggc gcggtgctcc actggcaggc gcatcacgtc tatgcgctcg
     1681 gcggcatcta tgacggcgtg cccggccttc ccggccaccc ggccggaacg catatcgagc
     1741 tgcgcagcgg cgacggcacc ttctccaacg tcgaagccgg catcatcggc aagggctaca
     1801 aacttggcgt cggcggctgg tatcgaacga cggatttcaa ggacatgctc ggcgaaccgt
     1861 acgatcgcaa ccacggtttc tacatcgtcg gttcgaagat gctgttcgga ggcgggggca
     1921 aaccgacgct cgatgttttc gtgcaactcg ggagcgccga agaggaccgc aacgaaatgg
     1981 gccgctacat cggcgccggc gtcgaactcc agcacttcat ttccggccgc ccgaacgatg
     2041 cactcggcct cggcatggcg cgcgtgcttc cgagtgatct cttccagcgt gcgacaccgg
     2101 gcgtcacgcg ccccgaaacc gtgttcgagg cgacgtatca ggcagcgatc tcgaagagcc
     2161 tcgtcatcca tcccgacgtg caatacatca cgaatccggg cgcagttccc gattacgaca
     2221 acgcgtgggt cgtcggattc cgattgcaat atcagatgca gggcggctga cgagccgtcc
     2281 gcaaggaagg aggaatcatg cgaagtttct ggaaatttct gctttcgcgc ccggtcgtat
     2341 ccggcgtcat catcggcgcc gctgcaggct ctgagcagga aacgcgagga gacatcgtca
     2401 tgccaggaaa aagccccgga ccgcaggtga aggacaagga ccgctacgag gcactccgcc
     2461 gtgaaggcga aagcaaggag aagtccgcga gaatcgccaa ttctccgcgc agcgagaccg
     2521 cccggaaggg tgggaaaagc agcgcctacg aggattggaa caaggacgat ctggaaaagc
     2581 gcgcgcggga gatcggcatc gaaggccgct cgcgcatgag caagaacgaa ctgatcaacg
     2641 ccctgcgaaa ccactgaaac tcggtctgat tccgagcgga cgcatcccga cggcccgacc
     2701 gcccgcttca accttcgggc tcttcctcgt ccgcaagttc gcatccgccg cgcctttcct
     2761 gcggatgcgt cgaagcgcct tgcccgtgag gcgtgagcct gggagcgatc agcgggtgcg
     2821 gcgacgaagc cgtcgtgccg ccgcttccgt acgggcgggt gatgatttcc agacggtgtc
     2881 cgctcggatc gtcgaaataa caaccgcggc cgtcgtccct ggaattgatt tcaccgccgc
     2941 gtttccggcg cggatcggcc cagtaccgga gtccgcgttc gcgaatgcgc gcgaatatct
     3001 cgtcgaattc gatttcgctc accagaaagg cgtagtgctg c
//
LOCUS       DASUJG010000376         3708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_353299, whole genome shotgun sequence.
ACCESSION   DASUJG010000376 DASUJG010000000
VERSION     DASUJG010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353299"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..1614)
                     /locus_tag="VFX38_08045"
     CDS             complement(88..1614)
                     /locus_tag="VFX38_08045"
                     /inference="COORDINATES: protein motif:HMM:NF025418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HEX5314836.1"
                     /translation="MTALRASRPFRPPFRRSLAAWACLLCVAAILCAPCIANADPWLA
                     PGDIGARQDVELLVDSGVIDVPVTTWPLPWGAISAALATVSPEALPPDEQAAYSRLVT
                     AIESVQAGADEFGYEAAAAPGRPALRWFGTTPRGKEEAGVSYAGYNGSVAFRFNLLGV
                     YGASDHQRGRFDGSYIAFALGNWILTAGQIDQYWGPAWSGSLIFGNNSRPVPGVLLSR
                     NVAEPFQTPLLHWLGPWTLSFFTGRLEDDRYIPHPYLIGARFAFRPLSGLEFGITRMA
                     QYGGEGRPQSLSCLFDALFGRTNKNKKTAADDCANELAGVDMRFHVPRTRFDFYMQGS
                     AEDSSGGIPTRWSDLFGLSTWGAIGDDGASYRAFLEYANTTANSFTTPIPNVTYENIR
                     YLSGYRYRQVDLGYPTDNDSELWTAGVTLQGVNGGNVTFLLRHGLLNGDNTNRNEPWG
                     GNKLAPVRTGLNAADVYYMPAFWGRHLSMGVGVSRWAAFGLPAETGFHGQIMWEQNFQ
                     "
     gene            complement(1863..1939)
                     /locus_tag="VFX38_08050"
     tRNA            complement(1863..1939)
                     /locus_tag="VFX38_08050"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1903..1905),aa:Arg,seq:acg)
     gene            complement(2100..>3708)
                     /locus_tag="VFX38_08055"
     CDS             complement(2100..>3708)
                     /locus_tag="VFX38_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEX5314837.1"
                     /translation="SARVMKKAVAWLLPYMEAEKDGKALAKGKILMATVKGDVHDIGK
                     NIVGVVLACNNYEVVDLGVMVPAAKLLEAARREQADIVGVSGLITPSLEEMAHVASEM
                     QRQGFTIPLLIGGATTSPTHTSVKVAPHYDGPTVYVKDASRAVGVVSRLLGRERASYV
                     KGIEANHETVRRHYAEKRRDERLVSFAEARANAPGLDWADYEPPQPRRPGVHVFADFP
                     LAEIRRYLDWTPFFHAWQLRGSYPKIFADPDKGEPARKLFADAEAMLERMIAERWTRA
                     SAVVGLFRAGRVGPESVALYGDAARSQELERLEFLRQQQKKPSGKFDYSLADLIAPAE
                     SGKTDWMGAFAVTAGIGTAERAQAFADAGDDYAAIMVKALSDRLAEAFAELMHAKVRR
                     ELWGYAPGEALDNEALIAERYQGIRPAPGYPACPDHTEKAKLWRLLDVTNNAAIEITE
                     SFAMWPAAAVSGWYLSHPAARYYAVGKIDREQLADYARRRAIPIEEAERWLAPNLAYE
                     GTAPSEAVAGDKPSAELSAPAAVARRA"
BASE COUNT          667 a         1238 c         1184 g          619 t
ORIGIN      
        1 acggaaaact tctccccgcg ggcgtaactc ggcagcgagg cagcgctcgg tcgtgcgccg
       61 ggatcatgcg cttacgcctg ccgttcttca ctggaaattc tgctcccaca tgatttgacc
      121 atgaaatccc gtttcggcgg gcaaaccgaa ggctgcccag cggctgaccc cgacgcccat
      181 ggacaggtgc cgcccccaga aagccggcat atagtacacg tcggccgcgt tcaggcccgt
      241 gcgcaccggc gccagcttgt tgccacccca cggctcgtta cggttggtgt tgtccccgtt
      301 gagaaggccg tgacggagga gaaacgtgac gttgccgccg ttcacgccct gcagggttac
      361 tcccgcggtc cacagctcgc tgtcattgtc cgtgggatag ccgagatcca cctggcgata
      421 ccgatatccg ctgagatagc ggatgttttc gtaggtgacg ttcgggatcg gcgtggtgaa
      481 cgaattggcg gtcgtgttcg cgtactcgag aaacgcccgg taactcgcgc cgtcgtctcc
      541 aatcgcgccc caggtcgaaa gaccaaacag atccgaccat cgggtcggaa ttccgcccga
      601 ggaatcttcc gccgagccct gcatgtaaaa atcgaagcgc gtgcgcggaa cgtgaaagcg
      661 catgtcaaca cccgcaagtt cgttggcgca gtcgtcggcg gcggtcttct tgttcttgtt
      721 ggtgcgcccg aacagcgcat cgaaaagaca actcaaactc tgcggccgac cttcgccgcc
      781 gtactgtgcc atgcgcgtga tgccgaactc gagccccgaa agggggcgga atgcgaaacg
      841 cgccccgata agatacggat gcggaatgta gcggtcatcc tccaatcgcc cggtaaaaaa
      901 cgacagggtc caagggccca accagtgcag gagcggcgtc tggaacggtt cggcaacgtt
      961 gcggctcagg agcactccgg ggaccgggcg gctgttgttg ccgaaaatca ggctgccgct
     1021 ccaggccggc ccccaatact ggtcgatctg gcccgcggtg agaatccagt tgccgagcgc
     1081 gaaggcgatg tagctcccgt cgaagcggcc gcgctgatga tcggatgccc catagacgcc
     1141 gagaagattg aagcggaagg caacgctgcc gttgtagccg gcataggaaa cgccggcctc
     1201 ttccttgccg cgcggcgtcg tgccgaacca gcgcagcgcc gggcgcccgg gggcggcggc
     1261 cgcttcgtat ccgaactcat cggcgcccgc ctgcaccgac tcgatcgccg taaccagacg
     1321 gctgtaggcc gcttgttcgt cgggcggcag cgcctccggc gagacggtcg cgagtgcggc
     1381 ggaaattgcg ccccagggca gaggccaagt cgtcaccggc acgtcaatca cgccagagtc
     1441 caccaggagt tccacgtcct gacgcgcgcc gatgtcgccg ggcgcgagcc aaggatcggc
     1501 gttcgcgatg cagggagcgc aaaggatggc ggcgacacag agcagacagg cccatgcggc
     1561 cagcgagcgg cggaacgggg ggcgaaaggg tcgggaggcg cgcaacgccg tcaacggctc
     1621 cgcccgactc gcgcctgcgc gatcacgatg cgaacccgca ataccctcgg ctccgcgctc
     1681 gcgcagacga aaccgcccgc gcatgcagcg gacagtgcat ttccgtcccc ggatttccac
     1741 acgccattca cgattaccgg gttgcccgca cggggaaaca ggcgaattat agacagattg
     1801 cgcagccggc tgcagacggg tccgcgcaat cggcgcgaag atcccgcaaa acggtggtgg
     1861 tttggcgcgc caggagagat tcgaactccc gaccccctgg ttcgtagcca ggtactctat
     1921 ccagctgagc tacgggcgca tccggcctga gcacacatcg ccccggatca tcgcccgccg
     1981 agttccggca gacgccgcga agcgatgcct ccgcccgtct attatcgcga gtctgtgccc
     2041 ggcgggtcaa cgcaaacaca gttttcgaat gcagcgatcg cgaccagcgg ccgcatccat
     2101 caggcccgac gcgccacggc agccggcgca gacagctccg cgctcggctt gtcgccggca
     2161 accgcctcgc tcggcgcagt cccttcatag gcaaggttcg gcgcgagcca gcgttcggcc
     2221 tcttcgatgg gaatcgcccg ccggcgcgcg taatcggcga gctgctcgcg atcgatcttg
     2281 ccgaccgcgt agtagcgcgc ggccggatgc gacaaatacc agccgctcac cgctgccgcg
     2341 ggccacatcg cgaaactctc ggtaatctcg atcgccgcgt tgttggtgac atcgaggagg
     2401 cgccagagct tcgccttttc cgtatgatcg gggcaggcgg gatagcccgg cgcggggcga
     2461 attccctggt agcgctcggc gatcagtgcc tcgttgtcga gcgcctcgcc gggggcatag
     2521 ccccagagtt cgcgccgcac tttcgcgtgc atgagctcgg caaaggcctc ggcgaggcga
     2581 tccgagagcg ccttcaccat gatcgcggcg taatcgtcgc cggcgtccgc gaaggcctgc
     2641 gcccgctcgg cggtgccgat accggcggta acggcgaagg ctcccatcca atccgttttg
     2701 ccgctctccg cgggggcaat caagtccgcg aggctgtagt cgaactttcc cgaaggcttc
     2761 ttctgctgct ggcgcaggaa ctcgaggcgt tcgagttcct gcgaacgcgc cgcatcgccg
     2821 tagagtgcga ccgattcggg accgacgcga ccggcgcgga agagaccgac aacggcgctc
     2881 gcccgggtcc agcgctcggc aatcatgcgc tcgagcatgg cctccgcatc ggcgaagagt
     2941 ttccgcgccg gctcgccctt gtcggggtcg gcgaagattt tcgggtagct tccgcgcaac
     3001 tgccaggcat ggaaaaacgg cgtccaatcg aggtaacggc gaatttccgc gagcgggaaa
     3061 tccgcgaaca cgtgaacccc cggcctgcgg ggttgcggcg gttcgtagtc cgcccaatca
     3121 agcccgggag cgttcgcgcg cgcctcggcg aaactcacga ggcgttcgtc gcgccgcttt
     3181 tccgcataat gccggcgcac ggtttcgtga ttagcctcga ttcccttcac atagctcgcg
     3241 cgctcgcgcc cgaggagacg cgacaccact cccacggcgc gcgaggcatc cttcacgtac
     3301 acggtcggcc cgtcgtagtg cggggcgacc ttgaccgaag tgtgagtcgg cgaggtcgtc
     3361 gccccgccga tcaaaagcgg aatcgtgaaa ccctggcgct gcatttcgga ggcgacatgc
     3421 gccatctctt ccagcgacgg cgtgatcagc cccgatacgc cgacgatgtc ggcctgttcc
     3481 cggcgtgcgg cttcgagcag cttcgccgcg ggcaccatga cgccgaggtc cacgacttcg
     3541 taattgttgc aggcaagcac gacgccgacg atgttcttgc cgatgtcgtg gacgtcgccc
     3601 ttcaccgtcg ccatcagaat cttgcccttg gcaagcgcct tgccgtcctt ttccgcctcc
     3661 atgtacggca gcagccaggc gaccgccttt ttcatgacgc gcgccgat
//
LOCUS       DASUJG010000377         2275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_232571, whole genome shotgun sequence.
ACCESSION   DASUJG010000377 DASUJG010000000
VERSION     DASUJG010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232571"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..1282
                     /locus_tag="VFX38_08060"
     CDS             44..1282
                     /locus_tag="VFX38_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019018838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5314838.1"
                     /translation="MKPDADPEPAESPAKRRGFVYASYAFLATMFGTTLPTPLYPLYR
                     HEFGLSQLMITVIYAVYAAGVIGALIFAGRWSDQLGRRRVMFAALGFSAASALVFLAA
                     GDIALLFLGRVLSGISAGIVTGTATVMIVELAPPKRRARATLVAAAVNMGGLGLGPLV
                     AGTLAQYAPLALRLSFLVDLALLAFAFLAVIRVPETVQIAAQPRLGAQRLHVPPEVRA
                     AFIPACIAGFAGFAVMGLFSAVAPAFLARILGLSNLLLAGLTVFTIFATSILGQLALE
                     WIPRDRALAIGCLILAAGAGLVGAGIGAASLALLFAGAAVSGFGQGLSFRAGLAAISA
                     RSPADRRAEITSSFFVVLYVALSLPVIGLGIAAASVGLRIAGVAFAIAVGILALVALL
                     ALWRVRPTDNTRRRADSETD"
     gene            complement(1423..>2275)
                     /locus_tag="VFX38_08065"
     CDS             complement(1423..>2275)
                     /locus_tag="VFX38_08065"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX5314839.1"
                     /translation="LTRPLVVWCILVGVLCIWHIPGIYGLALAHGWIHDLEHLSFIAA
                     GLAYWTVVIEPYGRRRALSYGATIVYVVSIGFIMGMIGAILTFAPIPFYAAQLKSVVA
                     FGLTPLADQELAGAIMWVPAEIIQLAALSVLALAWLREDERRAAHVRTVLPSSTATLR
                     SLAVVPIALVLVATLVGCGRHPDPYRPTVSGGNPQRGKQAIIHYGCDSCHTIPGVPGA
                     RGLVAPPLTHWRERNFVAGLLRNRPDNLVYWIRDPQKVIPGVAMPDLGVSEQEARDIA
                     AYLYTIH"
BASE COUNT          361 a          778 c          714 g          422 t
ORIGIN      
        1 ggccccattc ctttggcgca tcgggctgcg aatcgaaagg ccgatgaaac cggacgcaga
       61 cccggaaccg gcggaatcgc cggcgaagcg caggggcttc gtgtacgcga gctatgcgtt
      121 tctcgcgacc atgttcggga cgacgctccc gactcctctt tatccgctct accggcacga
      181 gttcggcctc tcccaactga tgatcacggt gatctatgcc gtctacgcgg ccggcgtcat
      241 cggcgcgctg atcttcgcag ggcgctggtc ggaccaactc gggcggcgcc gcgtcatgtt
      301 cgcggccctc ggcttctccg ccgcgagcgc gctcgtcttt ctcgccgccg gcgacatcgc
      361 gctcctgttt ctcggccgcg tgctttccgg aatctcggcc ggcatcgtga ccggaaccgc
      421 gacggtgatg atcgtcgaac tcgcgccgcc gaaacgtcgc gctcgcgcga ccctggtcgc
      481 ggccgcggta aacatgggcg gcctaggcct cggcccgctc gtcgccggca cactcgcgca
      541 gtacgcgccg ctcgccctgc gactgagttt tctcgtcgat cttgcgcttc tcgccttcgc
      601 cttcctcgcc gtgatccggg taccggaaac cgtgcagatc gccgcgcagc cgcgtctcgg
      661 ggcgcagcgc ctgcacgtcc cgccggaggt tcgcgcggct ttcattccgg cgtgcatcgc
      721 gggatttgcg ggtttcgccg taatggggct tttctcggcg gtcgcgccgg catttctcgc
      781 ccggattctc ggcttgtcca atctgctcct cgccggactc acggtgttca cgattttcgc
      841 gacctcgata ctcggacagc tcgcgttgga atggatcccg cgggatcgcg cactcgcgat
      901 cggatgcctg attcttgcgg ccggggccgg actcgtcggc gccgggatcg gcgccgcctc
      961 cctcgcgctt ctgttcgcgg gagccgccgt ctccggcttc gggcagggat tgtcgttccg
     1021 cgccggtctc gccgcgatca gcgcgagatc tcccgccgat cggcgcgccg agatcacttc
     1081 gagctttttt gtcgttctct acgtcgcgct gtcgcttccc gtcatcgggc tcggaattgc
     1141 ggccgcctcc gtcggccttc gcatcgccgg cgtcgcattc gcgattgccg tcggaatcct
     1201 cgcgcttgtc gccctgctcg ccctctggcg cgtccgtcca acggacaata cccgacgccg
     1261 ggccgactcc gaaacggact gaggcgactc gcaacgccgg aatcaatcat tccccaatct
     1321 gcgttcgaac gatttccgtc gcttgacgtt cattcgctcg cctccgacca agcttgccgc
     1381 cgattcaatc gcctggcgcc agcggcatgg cggcagatgg cgtcaatgaa tcgtgtaaag
     1441 gtaggccgcg atgtcgcgcg cttcctgctc gctcacgccg agatccggca tggcgacccc
     1501 gggtatcacc ttttgcggat cgcgaatcca gtaaacgaga ttgtcgggtc tgttgcgcag
     1561 caatccggcg acgaaattgc gctcacgcca atgggtgagc ggcggggcaa ccaggccacg
     1621 ggcgccggga acgccgggga tcgtgtgaca ggaatcgcag ccgtaatgaa tgattgcctg
     1681 tttcccgcgc tgaggattgc cgccgctgac cgtcgggcgg tagggatcgg gatgacggcc
     1741 gcagccgacg agcgtcgcca ccagcaccag cgcaatcggt accacggcga gactgcgcag
     1801 cgtcgccgtc gacgagggca acaccgttcg aacatgcgcc gcccgccgct cgtcttcgcg
     1861 aagccaggcg agagcgagca cgctgagcgc cgcgagttgg atgatctcgg ccggcaccca
     1921 catgatcgcc ccggccagct cctgatcggc tagcggcgtg aggccgaagg cgaccacgga
     1981 cttgagctgg gcagcgtaga acggaatcgg cgcgaaggtc agaatcgcgc cgatcatgcc
     2041 catgatgaag ccgatcgaga ccacatacac gatggtcgcg ccgtaactca atgcgcgccg
     2101 tcttccgtag ggttcgatca ccaccgtcca ataggcgaga cccgcggcga tgaagctcaa
     2161 atgttccagg tcgtgaatcc acccgtgagc aagcgcgaga ccatagatac cgggaatgtg
     2221 ccaaatgcac aagactccga cgagaatgca ccacacgaca agcgggcgcg tgaga
//
LOCUS       DASUJG010000378         3837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1698034, whole genome shotgun sequence.
ACCESSION   DASUJG010000378 DASUJG010000000
VERSION     DASUJG010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1698034"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1308
                     /gene="purF"
                     /locus_tag="VFX38_08070"
     CDS             <1..1308
                     /gene="purF"
                     /locus_tag="VFX38_08070"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008735948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX5314840.1"
                     /translation="MGIGHCRYPTAGGLGADEAQPLYTNSPFGIGLAHNGNLTNAKTL
                     SELIYRSDRRLLNTNSDSEVVLNVFAHELARSASLKVRAEDVFRAVASVHERCRGAYA
                     VVAMIVGQGLVAFRDPYGIRPLVLGQRNVDGGPEYMIASESVALDILGFERLRDVAPG
                     EAIYVERNGRLHARVMAQQTLLRPCLFEYVYLARPDSMMNDVSVYKARLRMGEKLAEK
                     ILREHPRHDIDTVIPIPETSRVSALPLAQKLRVPFREGFVKNRYIGRTFIMPGQEERQ
                     RSVRHKLNAIELEFRDKNVLLVDDSIVRGTTSRQIVEMARQAGARKVYFASAAPPVRH
                     PNVYGIDMPVASELIAHERSVEEINDYIGSDSLIYQDLPDLAEAVREGNPALDAFEDS
                     CFSGRYLTGDVTPDYLAELAAERADARKRARARAELANVLNED"
     gene            complement(1432..2385)
                     /locus_tag="VFX38_08075"
     CDS             complement(1432..2385)
                     /locus_tag="VFX38_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019020738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5314841.1"
                     /translation="MKNERKREVVVITGAGAGLGRATVREFARHGADIGLIARGADRL
                     EAARAEVEQCGGRALVSPADVADANAVEAAAERIESGLGPIAIWVNVAMATVFGYFTD
                     IAPEDFRRVTEVTYLGNVYATQAALKRMQPRNFGTIVQVGSALAYRSVPLQSAYCGAK
                     HAIQGYTESIRSELLKSKSRVHLTIVEMPALNTPQFDWCKNTMAKRARPVAPVFQPEV
                     GARAIYWAAHHRRRQLYVGFPAVKAIWANKFIPGILDRMLVSAIAGQQTDQSEDANRP
                     FNLYDPVAGDTGAHGRFDDESRTRSLELWFATHLLPSKTSH"
     gene            complement(2437..3522)
                     /locus_tag="VFX38_08080"
     CDS             complement(2437..3522)
                     /locus_tag="VFX38_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX5314842.1"
                     /translation="MPQLSKLAVSAYTIPTDAPESDGTLEWDSTTMVLVEVEAGGKTG
                     LGYTYGHQAIATLVRDKLADVITGREAMNTGGAWLAMTRAIRNLGRPGLCSMAIAAVD
                     LALWDLKARLLDLPLARLLGALRDEVPVYGSGGFTSYSDSKLCEQLAAWVEAGIPRVK
                     MKIGREPGRDIERIMAAREAIGADAQLFVDANGAYDRKQALSIAQAFSELGVSWYEEP
                     VSSDDLEGLRLIRDCAPPAVEITAGEYGYRAEYFRVMLEAGAVDVIQADVTRCAGVTG
                     FLAAAAIADAHHIPLSGHCAPAMHLHPGCAVKPLRHLEYFHDHVRIEQLLFDGVSKPD
                     GGVLRPDLARPGHGLELKRADADKYLL"
     gene            complement(3524..>3837)
                     /locus_tag="VFX38_08085"
     CDS             complement(3524..>3837)
                     /locus_tag="VFX38_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018916170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HEX5314843.1"
                     /translation="YEASQDVPDFPYAEYAKLLGLEGIAVTETDQIGPAWDRALSAAK
                     PTVLEVSTDPDVPPLPPHITKEQAKAYLSAMLHGDPDALGIVKASVKQFAADFSTHFK
                     S"
BASE COUNT          656 a         1241 c         1252 g          688 t
ORIGIN      
        1 atggggatcg gccattgccg ctacccgacc gccggcgggc tcggcgccga cgaagcgcag
       61 cctctgtaca ccaattcgcc cttcggtatc gggctcgcgc acaacggcaa tcttaccaac
      121 gccaagacgc tttcggaact catctaccgt tccgatcgca ggcttctcaa caccaactcg
      181 gattccgagg tcgtgctcaa cgtgttcgcg cacgaactcg cgcgctcggc ctcgctcaag
      241 gtgcgggccg aagacgtgtt ccgggccgtg gcgagtgtgc atgaacgctg ccgtggcgcc
      301 tatgcggtgg tcgcaatgat cgtcgggcag gggctcgttg cgtttcgcga cccgtacgga
      361 atccgcccgc tcgtcctcgg gcagcgcaac gtggacggcg gtcccgaata catgatcgcc
      421 tcggaaagcg tcgcgctcga cattctcggc ttcgagcgcc tgcgcgacgt cgcgccgggg
      481 gaggcgatct acgtcgagcg caacggtcgc ctgcacgcgc gggtgatggc gcagcagacg
      541 cttttgcggc cctgcctctt cgaatacgtg tatctcgcgc ggcccgactc gatgatgaac
      601 gatgtgtccg tgtacaaggc ccgccttcgc atgggagaga aactcgccga aaagattctt
      661 cgcgagcatc cgcgccacga catcgacacc gtgattccga tccccgagac cagccgcgtg
      721 tcggcgcttc cgctcgcgca gaagctgcgc gttcccttcc gcgaaggctt cgtcaagaat
      781 cgctacatcg ggcgcacctt catcatgccg gggcaggagg aacgtcaacg ctccgtgcgc
      841 cacaagctca acgcgatcga actcgagttc cgcgacaaga acgtgctgct ggtggacgat
      901 tcgatcgtgc gcggtacgac ctcgcgtcag attgtggaga tggcgcgcca ggccggggcg
      961 cgcaaggtgt atttcgcctc cgccgcgccg ccggtgcgtc atccgaacgt atacggcatc
     1021 gacatgccgg tcgcctccga actcatcgca cacgagcgca gtgtcgagga gatcaacgac
     1081 tacatcggct cggactcact gatctatcag gaccttcccg atctcgccga ggccgtgcgc
     1141 gagggcaatc ccgcgctcga tgccttcgag gactcctgct tcagcggccg ttatctcacc
     1201 ggcgacgtga cgcccgatta tctcgccgaa ctcgccgccg aacgcgccga tgcgcgcaag
     1261 cgcgcccgcg cccgcgccga actcgccaac gtgctcaacg aagattgacc ctcgcgcttc
     1321 gttccgaagg gcaaaggcgg gacatccccc cgcgaggacg cggccccctt cgttccgaag
     1381 ggggcgaagc tactcggcga agacccttcc ccgttccgaa ggaaagggga ttcagtggga
     1441 tgtcttcgaa ggcaacagat gcgttgcgaa ccataactca aggctgcgcg ttcgcgattc
     1501 gtcatcgaaa cggccgtgcg cgccggtatc gccggccaca ggatcataga ggttgaaggg
     1561 ccgattcgca tcttcggact gatcggtctg ctgcccggcg attgccgaga cgagcatgcg
     1621 atcgagaatt cccggaatga atttgttggc ccagatcgct ttcacggccg ggaatcccac
     1681 gtaaagctga cggcggcggt gatgcgccgc ccagtagatc gcccgcgcgc cgacctccgg
     1741 ctgaaacacc ggcgcgaccg gccgtgcacg cttcgccatg gtgtttttgc accagtcgaa
     1801 ctgcggcgtg ttcagcgccg gcatctcgac gatcgtcagg tgcacgcggc tcttcgactt
     1861 caggagttcg ctgcggatgg actcggtgta gccctgaatg gcgtgtttgg caccgcaata
     1921 cgccgattgc aagggaaccg atcgataggc gagcgcggaa ccgacctgga cgatggtgcc
     1981 gaagttgcgt ggctgcatcc gtttcagagc agcttgggtg gcgtaaacgt tgccgagata
     2041 ggtgacctcc gtcacgcgcc ggaaatcctc gggcgcaatg tcggtgaagt atccgaagac
     2101 ggttgccatc gcgacgttga cccagatcgc gatcggtccg agtccggatt cgatgcgctc
     2161 ggcggccgct tcgacggcgt tggcatcggc gacgtctgcc ggggagacga gggcgcggcc
     2221 gccgcattgt tccacctcgg cccgcgccgc ctcgagccgg tctgcgccgc gcgcgatcag
     2281 tccgatgtcg gcgccgtggc gcgcgaactc ccgcacggtc gcgcgcccca ggccggcgcc
     2341 cgccccggtg atcacgacaa cctcgcgttt tcgttcgttc ttcatggcgc ctccatggct
     2401 ggatgccggc agcaccgctg cgcggcttgt gccgatctac aggagatatt tgtcggcatc
     2461 ggcacgcttc agctcgaggc cgtggccggg tcgggcgaga tcgggcctga gcacgccgcc
     2521 gtcgggtttg ctcacgccat cgaagagcag ttgctcgatc cgtacgtgat cgtgaaagta
     2581 ttcgagatgg cgcagcggct ttaccgcgca gccgggatgc agatgcatcg ccggcgcgca
     2641 gtgcccggag aggggtatgt ggtgagcgtc ggcgattgct gcggccgcga gaaaaccggt
     2701 gactccggcg cagcgcgtga catcggcctg gattacgtcc acggcgccgg cctccagcat
     2761 gacgcgaaag tactcggcgc gatagccgta ctcgccggcg gtgatttcaa ccgcgggcgg
     2821 cgcgcagtcg cggatcaggc gcaaaccttc caggtcgtcg gaggaaacgg gttcttcgta
     2881 ccagctcacg ccaagctcgg agaatgcctg ggcgatcgag agcgcctgct tgcgatcgta
     2941 ggcgccgttc gcgtccacga aaagctgagc gtctgccccg atcgcctcgc gcgccgccat
     3001 gatgcgttcg atgtcgcggc cgggttcgcg cccgatcttc atcttcacgc ggggaatgcc
     3061 ggcctcgacc caagccgcca attgttcgca gagcttcgag tcggagtagg acgtgaagcc
     3121 accgctgccg tacaccggca cttcgtcgcg caatgcgccc aacagtctcg cgagcgggag
     3181 atcgagcagc cgcgccttga gatcccagag cgcgagatcc accgccgcga ttgccatcga
     3241 acacaggccc gggcgcccga ggttgcgaat cgcccgcgtc atggcgagcc aggcgccgcc
     3301 ggtattcatg gcctcgcggc cggtgatcac gtcggcgagc ttgtcgcgga cgagcgtcgc
     3361 aatcgcctga tggccgtagg tgtagccgag gccggtcttg ccgccggctt cgacttcgac
     3421 cagcaccatc gtggtcgaat cccactcaag cgtgccgtcg gactccggtg catcggtcgg
     3481 gatcgtgtag gccgataccg cgagcttcga gagctgcggc atctcagctt ttgaagtggg
     3541 tcgaaaaatc ggcggcgaat tgcttcaccg aggccttgac gatgccgagc gcgtccggat
     3601 cgccgtggag cattgcggac agataggcct tcgcctgctc tttggtgatg tgcggcggaa
     3661 gcggcggcac gtcgggatcg gtgctgacct cgagcaccgt cggtttggcg gcggacaggg
     3721 cgcgatccca agccggtccg atctggtcgg tttcggtgac cgcaatgcct tcgaggccga
     3781 gcaatttcgc gtattcggcg tagggaaaat cgggcacgtc ctgggaggct tcgtatt
//
LOCUS       DASUJG010000379         4085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_108450, whole genome shotgun sequence.
ACCESSION   DASUJG010000379 DASUJG010000000
VERSION     DASUJG010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108450"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="VFX38_08090"
     CDS             complement(<1..803)
                     /locus_tag="VFX38_08090"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5314844.1"
                     /translation="MVTRFNLSEWALHHRQMVVFMLVVFTVAGLFAYFHLGQEEDPTF
                     TVRSMTVKAYWPGASVYDMENQVTDQFEQTLQTIGQIDHVQSWARAGATLLTITVRDD
                     VPPSQVPGVWYDVRKKIGDMEYQLPKGVQGPFFNDEYGETFGNIYALTGDGFSYQQLK
                     QFAKSLRAQILTIPAVDQVNYVGDQDQEIYIEMSNAKLASLGIDPMQILKVLNETNGV
                     EPAGVIQTGTDQVQLRVSGEFESLDTIRDIGIRSGDQVFRLGDIATVARG"
     gene            complement(807..1913)
                     /locus_tag="VFX38_08095"
     CDS             complement(807..1913)
                     /locus_tag="VFX38_08095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5314845.1"
                     /translation="MRELRSQPLGLTIAVAVAAMLLAACGKEKADPAPVRPVQTMVVG
                     RYVETAGATYSGQVQAQYSSQQGFQVAGIVAKRITEVGDRVAVGAPLLQLDLSNLQYN
                     LQQAKAQLDAARSQAAQAKVNLGRSKALVDQNFISQTEYDQSEVDYQTALAQQQAAQA
                     QYGEAENALGYGTLRAAVPGIVTSINVDVGQVVQAGEDAVDIAQNGDREVVISVPESR
                     VYQVRHARDLTASLWAIPDVTYKARLRVLYPDVNQSTRTYDGHVTLLNPDATVRLGMT
                     AYVHVPGTVAHRAYRVPLTALYNRDGASLLWVMNAKDSTVSARSVKICALQGDSALIS
                     SGIAPGDVIVTAGAQLLHAGQKVNPVGTYFPQSQ"
     gene            complement(1970..2677)
                     /locus_tag="VFX38_08100"
     CDS             complement(1970..2677)
                     /locus_tag="VFX38_08100"
                     /inference="COORDINATES: protein motif:HMM:NF023197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HEX5314846.1"
                     /translation="MKLLFPAFAILLAGLAAGCSSTEQAKSVQPNGFLAPYQSQLEKG
                     QRGKEALLVYRNPDADWASYHAILLEPVQLWNDPGEKLSAEDQKDLQELVDSFYATLY
                     NKLSQDYAMVAAPAPGVMRIQIAISHGEPSHTGLALVSKAILPVKLMNSLWSFASGKP
                     AFTGEVTIEAVVKDSASDKVLGVGADRRVGGLKLFAPNAFSSWGDVKNSLNFYAAAAV
                     WRLCVLRGDSHCVKPKA"
     gene            2827..>4085
                     /locus_tag="VFX38_08105"
     CDS             2827..>4085
                     /locus_tag="VFX38_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEX5314847.1"
                     /translation="MTAAPAQAAPPRSDSRLAAEVARRRTFAIVSHPDAGKTTLTEKL
                     LLFGGAIQLAGTVKARKSRRHVTSDWLEIEKQRGISVASSVMQFPYRDHVINLLDTPG
                     HQDFSEDTYRVLTAVDAALMVIDAAKGVEAQTIKLLEVCRMRATPVITFINKMDREVR
                     EPLALLDEIETVLGMDCAPATWPVGMGKRFGGVYDLAADRIRRFKPGEERAADDLEVV
                     HGLDNPQLERLFGLDAETLRTELELVRGAGTGFDEPAFLAGRQTPVFFGSAINNFGVR
                     EILDALLAWAPAPQPRDGGPRIVRPDEPDFSGIVFKIQANMDPQHHDRIAFLRLCSGR
                     YSPGLRAKHLRSGKEIRIANAVTFMAQERNRLDSAVAGDIIGIQNHGLLGIGDVLSEG
                     EELRYAGIPHFAPELFRRVRLRNPLRA"
BASE COUNT          703 a         1350 c         1291 g          741 t
ORIGIN      
        1 ccacgggcga cggtcgcgat gtcgccgagg cgaaatacct gatctcccga acggatgccg
       61 atatcgcgga tggtgtcgag cgattcgaat tcgccgctca cccgcagctg cacctgatcg
      121 gtgccggtct ggatgacccc cgccggctcg acaccgttgg tttcgttcag caccttgaga
      181 atctgcatgg gatcgatgcc gagcgaggcg agcttcgcat tcgacatttc gatgtagatt
      241 tcctggtcct ggtcgccgac gtagttcacc tgatcgaccg ccggaatggt caggatctgc
      301 gcacgcaggc tcttcgcgaa ctgcttgagt tgctggtagc tgaatccgtc tccggtcagc
      361 gcataaatgt tgccgaaggt ttctccgtat tcgtcgttga aaaacggccc ctgaacgccc
      421 ttcggcaact ggtattccat gtcgccgatc tttttccgca cgtcgtacca gacgccgggc
      481 acctgcgagg gcggcacgtc gtcgcgcacc gtgatcgtga ggagcgtcgc cccggccctg
      541 gcccagctct gcacgtgatc gatctgcccg atcgtctgca gggtctgttc gaactggtcg
      601 gtgacctggt tttccatgtc gtacacgctc gcgccgggcc agtaggcctt gacggtcatc
      661 gagcggacgg tgaaggtcgg gtcttcctcc tggccgagat ggaaatacgc gaacaggccg
      721 gcgaccgtaa agaccaccag catgaacacg accatctggc ggtgatgaag ggcccactcc
      781 gacagattga agcgggtgac cacgccctac tggctctgcg ggaaataggt tccgacggga
      841 ttcaccttct gccccgcgtg aaggagctgc gcgcccgcgg tgacgatcac gtctccgggc
      901 gcgattccgg aagagatgag ggcgctgtcg ccctgaagcg cgcagatttt cacgctgcga
      961 gcggataccg tcgaatcctt cgcgttcatc acccagagga gcgaagcgcc gtcgcggttg
     1021 tacagtgccg tgagcggcac ccgataggca cgatgcgcga ccgttcccgg cacgtggacg
     1081 tatgccgtca tgccgagacg caccgtcgcg tcgggattga gcagcgtaac gtgaccgtcg
     1141 taggtgcggg tcgactgatt cacgtcggga tagagaacgc gcagccgcgc cttgtaggtc
     1201 acgtcgggga tcgcccacag gctggcggtg aggtcgcgcg catggcgaac ctggtacacc
     1261 cgcgattcgg gcacgctgat gaccacctcg cgatcgccgt tctgggcaat gtccaccgcg
     1321 tcctcgcccg cttgcaccac ctgcccgacg tccacgttga tggaagtgac gatgcccggg
     1381 accgcggcgc gcagcgttcc atatccgagc gcgttttcgg cctcgccgta ttgcgcctgc
     1441 gccgcctgct gctgcgcgag cgccgtctgg taatcgactt cgctctggtc gtattcggtc
     1501 tggctgatga agttctgatc caccagcgcc ttggaacgtc cgagattgac cttggcctgc
     1561 gcggcctgac tgcgcgccgc gtcgagttgc gccttcgcct gttgcaggtt gtactgaaga
     1621 ttgctgagat cgagctgcag aagcggagcg ccgacggcaa cgcggtcgcc tacttcggtg
     1681 atgcgcttcg cgacgatgcc cgcgacctgg aatccctgct ggctcgagta ctgcgcctga
     1741 acctggcccg aataggtcgc gccggccgtc tccacatagc ggcccacgac catggtctgc
     1801 accgggcgca ccggtgcggg gtcggctttt tccttgccgc aggcggcgag gagcatcgcc
     1861 gcgacagcca ccgcgatcgt caggccgagc ggttgcgagc gcagttcacg cactatcgcg
     1921 gttcccgatt cattgtccgc ccgtaacgct ccccggcgtc accgcccggt caggctttgg
     1981 gtttcacgca atggctgtcg ccccgcagca cgcacaggcg ccagacggcc gcggcggcgt
     2041 agaagttgag gctgttcttc acgtcgcccc aggagctgaa cgcgttcggc gcgaagagct
     2101 tcaggccgcc gacgcgtcga tcggcaccga cgcccagcac cttgtcgctc gcagaatcct
     2161 tcaccaccgc ctcgatcgtc acctcgccgg tgaaggccgg cttgccgctc gcgaacgacc
     2221 acagcgaatt catgagcttg accggcagga tcgccttgga cacgagcgcg aggccggtgt
     2281 gcgaaggctc gccatggctg attgcgatct ggatgcgcat cactcccgga gccggggccg
     2341 ccaccatcgc gtagtcctgc gagagcttgt tgtacaaggt cgcgtagaag ctgtcgacga
     2401 gttcctgcaa gtccttctgg tcttcggcgg agagcttctc gccgggatcg ttccacagtt
     2461 ggacgggctc gaggaggatc gcgtgatagg acgcccagtc ggcgtccggg ttgcggtaga
     2521 cgagcagggc ctccttgccg cgctgcccct tctcgagctg cgactgatag ggagcgagaa
     2581 agccgttcgg ctggacgctc ttcgcctgct cggtgctgga gcagccggca gcaagcccgg
     2641 cgaggaggat tgcaaatgcg gggaaaagca gcttcattcc ggcagtacca ggagtgcggc
     2701 agcccgcgtt ggccggtata atattcctcg ccgcgcgcgt tgtcgagcgc tgcgtaatcg
     2761 cggccccaag cgacggcgcc ctcctccgcg cgatccgcgg ctcccttcgg cgaaccggac
     2821 tcccccatga cagcagcccc ggcccaggcc gcgcctcccc gcagcgattc gcgactcgcc
     2881 gccgaagtcg cgcggcggcg caccttcgcc atcgtttcgc accccgatgc gggcaagacg
     2941 acgttgaccg aaaagctgct gctcttcggc ggtgcgatcc agctcgccgg caccgtcaag
     3001 gcgcgaaaaa gccgacgcca cgtgacctcg gactggctcg agatcgaaaa gcagcgcggc
     3061 atctcggtcg cgagttccgt gatgcagttc ccctatcgcg atcacgtcat caacctgctc
     3121 gacacgccgg gccatcagga cttctccgaa gatacctacc gcgtgctgac ggcggtggat
     3181 gcggcgctga tggtgatcga tgcggcgaaa ggcgtggagg cacagaccat caagctgctc
     3241 gaggtttgcc gcatgcgggc aacgcccgtc attaccttca tcaacaagat ggaccgggag
     3301 gtgcgcgagc cgctcgcact gctcgacgaa atcgaaaccg tgctcggcat ggattgcgcg
     3361 cccgcgacct ggcccgtcgg catgggaaaa cgcttcggcg gcgtttacga tctcgccgcg
     3421 gatcgcatcc gtcgcttcaa gcccggcgaa gagcgcgccg ccgatgatct cgaagttgtc
     3481 cacggactcg acaacccgca actggaacgg ctgtttggac tggacgccga aacactgcgc
     3541 acggaattgg aactcgtgcg cggcgccggc accggattcg acgagccggc ctttctcgcc
     3601 ggaaggcaga cgccggtgtt cttcggttcg gcgatcaaca acttcggcgt gcgcgaaatc
     3661 ctcgacgccc tcctcgcctg ggcgccggcg ccgcaaccgc gcgacggcgg gcctcgcatc
     3721 gtgcgcccgg acgagcccga cttcagcggc atcgtattca agattcaggc gaatatggac
     3781 ccgcaacacc atgatcgtat tgcatttcta cggctctgtt ccggccgcta ttcacccggc
     3841 ttgcgcgcga aacatttgcg cagcgggaag gaaatccgca tcgccaacgc cgtgaccttc
     3901 atggcacagg aacgcaaccg actggacagc gccgtcgccg gcgacatcat cggcatccag
     3961 aatcacggcc tgctcggcat cggcgacgtg ctgagcgaag gcgaagaact gcgctacgcc
     4021 ggcatcccgc acttcgcccc ggaactcttc cgccgcgtgc gcctgcgcaa tccgctgcgt
     4081 gccaa
//
LOCUS       DASUJG010000380         2097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_233129, whole genome shotgun sequence.
ACCESSION   DASUJG010000380 DASUJG010000000
VERSION     DASUJG010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233129"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VFX38_08110"
     CDS             <1..759
                     /locus_tag="VFX38_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEX5314848.1"
                     /translation="IETPRLLLNSANTQFPDGLANSSGLVGKYLMAQTNQAAWGVMED
                     EIRWYKGPPSLAITEHWNYTDTGKDFFGGYAYMSQGPLPQAWASTLAGNRGLWGEALL
                     REMEKYNHQAGLKIVGEVLPQEANRVTLADEKDQYGLPIARVTYSLCDNDKALVKHAL
                     GFMSRSLEAIGASDIWTEDDDTAHLNGTARMGDDPATSVVNADCRSWDIPNLWICDGS
                     VFPTVGGVNPSLTIQAIACRTGDRIREMAARGVL"
     gene            complement(776..>2097)
                     /locus_tag="VFX38_08115"
     CDS             complement(776..>2097)
                     /locus_tag="VFX38_08115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5314849.1"
                     /translation="RLGFFRATAFRVAAIYALVYAVLTGVLVFIVYVHATNVIETQIR
                     EGLMDESTAISSLLLDHGPRAVSDAIHARSARRIAAAGPTVTDPGRRYYILASGEGKV
                     LTGDIPDWPEAAPASGWFRFRLQGRGEILAVVTPLQSGMKLLVGQSLVTTDALARTVK
                     LWVLLSAALALLVGLIGGGIVGSRVMHRIRAASATAERIQAGQLSERLATGGPAEHAA
                     LARTFNAMLERIEAAVIGLRDLAARTAHEMQHPLTRADQALARAEQSEDRAMIDAEIA
                     AARREIQELAQRNEALLRLARLEGDSAREFFREFDLARMVTDVSELYAPLAEERGRSL
                     VLAAMRELRIVGDRQLLAQAFANLLDNALKYAPPGPIGVRIEHAANDAVLEVRDGGAG
                     PGSDRTLRGSGLGLPIARAIAQLHGGRLEFAHDAAGFGVRLILRARP"
BASE COUNT          390 a          665 c          707 g          335 t
ORIGIN      
        1 atcgaaacgc cgcggctcct gttgaattcc gcgaacaccc aattcccgga cggcctcgcc
       61 aacagctcgg gactggtcgg aaaatatctc atggcgcaaa ccaaccaggc ggcctggggc
      121 gtgatggagg acgaaatccg ctggtacaag gggccccctt cgcttgcgat taccgagcat
      181 tggaattaca cggataccgg aaaggacttc ttcggcggtt acgcctacat gagccagggt
      241 ccgctcccgc aggcctgggc ctcgacgctc gccggaaacc gcggcctgtg gggcgaggcg
      301 ttgctgcgcg aaatggagaa atacaaccat caggcggggc tcaagatcgt cggcgaggtg
      361 ctgccgcagg aagcgaaccg cgtgacgctc gccgacgaaa aggaccagta cgggctcccg
      421 atcgcgcgtg tcacctattc gctttgcgac aacgacaagg cgttggtgaa acacgcgctc
      481 ggcttcatgt cgcgaagcct tgaggcgatc ggcgcgagcg acatctggac cgaagacgac
      541 gacacggcgc atctcaacgg gacggcgcgc atgggcgacg acccggccac gagcgtcgtc
      601 aatgccgatt gccgcagttg ggacatcccc aacctgtgga tctgcgacgg ttcggttttc
      661 ccaaccgtag gcggagtgaa tccctcgctg acgatccagg cgattgcctg ccgcaccggc
      721 gatcgaattc gggaaatggc ggcccgcgga gtgctttgat ccggatttga tgcactcacg
      781 gccgggcgcg gagaataagg cgcacgccga aacccgccgc atcgtgcgcg aattccagac
      841 gtccgccgtg aagctgggcg atggcgcggg caatcggcaa gccgagtccc gaaccgcgta
      901 acgtcctgtc ggagccggga ccggcgccgc cgtcgcgtac ttcgagcacg gcatcgtttg
      961 cggcgtgctc gatgcgcacg ccgatcggtc cgggcggagc gtacttcaag gcattgtcga
     1021 ggagattggc gaaggcctgc gcaagcaact gacgatctcc cacgatgcgc agctcgcgca
     1081 tggctgcgag aacgagactg cggccacgtt cttcggcgag cggtgcatac agttcgctca
     1141 cgtcggtgac catgcgagcg agatcgaact cgcggaagaa ttcgcgcgcg gaatcgcctt
     1201 cgagccgggc gaggcgcagc agcgcctcgt tgcgttgcgc gagttcctgg atttcccggc
     1261 gcgccgcggc gatttcggca tcgatcatcg cgcgatcttc cgattgttcg gcccgcgcga
     1321 gcgcctgatc ggcacgcgtc agcggatgtt gcatctcgtg cgccgtgcgt gcggcgagat
     1381 cgcgcaggcc gatcaccgct gcctcgatgc gctcgagcat ggcattgaag gtgcgcgcga
     1441 gcgcggcatg ttccgccggc ccaccggtcg cgagccgctc gctcaactgt cccgcctgaa
     1501 tgcgttcggc cgtcgcgctc gccgcacgga tgcggtgcat cacccgggag ccgacgatgc
     1561 cgccgccgat gagtccgacg agaagagcga gcgcggcact gaggagcacc cacagcttca
     1621 cggtgcgggc aagagcgtca gtggtgacga gcgattgccc gacgagaagc ttcatgccac
     1681 tttgcagggg cgtgaccacg gcgagaattt cgccgcggcc ctgcaggcgg aatcggaacc
     1741 agccgctggc aggcgcggct tcgggccagt cgggaatgtc ccccgtcagc accttgccct
     1801 cgccgctcgc gagaatgtag taacggcgcc cgggatcggt caccgtgggt ccggcggcgg
     1861 caatgcgccg cgcgctgcgc gcatggatcg catcgctgac cgcgcggggt ccgtgatcga
     1921 ggagcagcga agagatggcg gtgctttcgt ccatcagacc ctcgcggatc tgggtctcga
     1981 tcacgttggt cgcgtgcaca tagacgatga ataccagaac cccggtgagg acggcataca
     2041 cgagggcgta tattgcggcg acgcgaaacg ccgtcgcgcg gaaaaatccc aaccgca
//
LOCUS       DASUJG010000381         2428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_719857, whole genome shotgun sequence.
ACCESSION   DASUJG010000381 DASUJG010000000
VERSION     DASUJG010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719857"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /gene="mnmA"
                     /locus_tag="VFX38_08120"
     CDS             complement(<1..692)
                     /gene="mnmA"
                     /locus_tag="VFX38_08120"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX5314850.1"
                     /translation="MKVVVGLSGGVDSSVAALLLKRAGHEVTGLFMKNWEDDDDDAYC
                     STREDLVDAAAAAEVIGIELEAVNFAAEYKDRVFAEFLREYSAGRTPNPDVLCNAEIK
                     FKAFLDHAMRLGAEKIATGHYAGIRKNNDAFELLKGRDAGKDQSYFLHRLTQEQLART
                     VFPLGDLKKAEVRRIAREAGLPNHARKDSTGICFIGERPFREFLNKYLSRSPGPIVDE
                     RGSALGQHIGLA"
     gene            complement(689..1144)
                     /locus_tag="VFX38_08125"
     CDS             complement(689..1144)
                     /locus_tag="VFX38_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX5314851.1"
                     /translation="MKDIWRPSVTVAAVIERDGRFLFVEERIDGRLVLNQPAGHLDPG
                     ESLADACAREVLEETAHRFRPSALVGVYRWRYAPKDVTFLRFCFAGEVLSEERGRPLD
                     REIVRLHWLDPGELKRRQAEQRSPLVLKCVEDYLAGRRYPLELLSPEFA"
     gene            complement(1141..2274)
                     /locus_tag="VFX38_08130"
     CDS             complement(1141..2274)
                     /locus_tag="VFX38_08130"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX5314852.1"
                     /translation="MRAGRDIVSFCIGQPDFPTPGNIQDAAIKAIRAGRHGYTPSAGI
                     DELRAAAARDLGSRRGIEVDAGDVVVAGGAKPFIAYTIASVTDFGAGDEVIYPVPGFP
                     IYESQIVANGAVPVPIFLKESRGFAFDPAELEAKITDKTRLLILNSPQNPTGGILDAE
                     ALDAIAAILVEHPQVWVYADEIYSRLAYAEEFFSIAQVPGMAERTIICDGASKTWAMT
                     GWRIGFAANAALAPVFTRWITNTDSCASQISQWAALEAINGPQEAAEAMKQSFLERRN
                     LIAGLLNEVPGVSCRRPGGAFYAWPNITEACRLIGAADSEEFRKRLLNEAGVAVLADI
                     HFGARVPGEGQHLRFSYAASNEAIRAGVARMAEFIRKNTQKAA"
BASE COUNT          404 a          824 c          795 g          405 t
ORIGIN      
        1 aaggcgaggc cgatgtgctg gcccagagcg ctgccgcgct catcgacgat gggccccggg
       61 gaccgggaaa gatacttgtt gagaaactcc cggaacggcc gctcaccgat gaagcaaatg
      121 ccggtcgagt ccttccgggc atggttcggc aggcccgcct cgcgggcgat gcgccgtacc
      181 tcggctttct tcagatcgcc gagcggaaac acggtgcgtg caagctgctc ctgggtgagg
      241 cggtgcagga agtagctctg gtccttgccg gcgtcccggc ctttcagcaa ttcgaatgca
      301 tcattatttt tgcggattcc ggcgtaatgc ccggtcgcga tcttctcggc gcccaggcgc
      361 atggcatggt cgaggaaggc cttgaacttg atctcggcgt tgcacagcac gtccgggttg
      421 ggcgtgcggc cggccgagta ctcgcgcagg aactcggcga acacccggtc cttgtactcg
      481 gcggcgaaat tgaccgcttc gagctcgatg ccgatcacct cggccgccgc cgccgcatcg
      541 acgaggtcct cgcgcgtcga gcagtacgcg tcgtcgtcgt catcctccca gttcttcatg
      601 aacaggcctg tcacctcgtg gcccgcgcgc ttgagcagca gcgccgccac cgaggaatcc
      661 accccgcccg acaggccgac cacgactttc acgcgaactc cggcgacagc agctcgagcg
      721 gatagcgccg gcccgcgagg tagtcctcga cgcacttgag cacgagcggc gagcgctgct
      781 cggcttgcct gcgcttcaat tcacccggat ccagccaatg caggcgcacg atctccctgt
      841 cgagcggccg gccccgctct tcggaaagga cttctcccgc gaagcagaag cgaagaaacg
      901 tcacgtcctt cggcgcgtag cgccagcggt aaacgccgac cagcgcgctg ggacggaaac
      961 gatgcgcggt ctcctcgagc acctcgcgcg cgcaggcgtc ggcaagcgat tcgcccgggt
     1021 cgaggtggcc ggcgggctgg ttgagcacga ggcggccgtc gatgcgctct tcgacgaaga
     1081 gaaagcgccc gtcgcgctcg atgacggcag cgaccgtgac gctcggccgc cagatgtcct
     1141 tcatgccgcc ttctgggtgt tcttccggat gaactcggcc atgcgggcga cgcccgcgcg
     1201 gatcgcctcg ttggaagcgg catacgagaa gcgcaggtgc tggccttcgc ccggcacccg
     1261 ggcgccgaag tggatgtcgg cgagcactgc cacccccgcc tcgttcagca ggcgcttcct
     1321 gaactcctcg gagtcggccg cgccgatcag gcggcaggct tcggtgatgt tcggccaggc
     1381 gtagaaggcg ccgccgggcc gcctgcagct cacgcccggc acctcgttca gcaggcccgc
     1441 gatcaggttg cgccgctcga ggaagctctg cttcatcgcc tcggccgcct cctggggccc
     1501 attgatcgcc tcgagcgccg cccactggct gatctgcgag gcgcaggaat cggtgttggt
     1561 gatccagcgg gtgaacaccg gggcgagcgc cgcgttcgcg gcgaagccga tgcgccagcc
     1621 cgtcatcgcc caggttttcg acgcgccgtc gcagatgatg gtgcgctcgg ccatgcccgg
     1681 cacctgcgcg atcgagaaga actcctccgc ataggcgagg cgcgagtaaa tctcgtccgc
     1741 gtacacccac acctgcgggt gctcgacgag gatcgcagcg atggcatcga gcgcctccgc
     1801 gtcgaggatc cccccggtcg gattctgcgg cgagttcaag atcaggagtc gcgttttgtc
     1861 ggtgatcttc gcttcgagtt cggcgggatc gaacgcaaag ccacgcgact ccttgaggaa
     1921 aatcggaacc ggaacggcgc cgttggcgac gatctgcgac tcgtagatcg ggaagcccgg
     1981 aaccgggtag atcacctcgt caccggcccc gaaatcggtc accgaagcga tcgtgtaggc
     2041 gatgaaaggt ttcgccccac ccgcgaccac cacgtctccc gcatcgacct cgatgccgcg
     2101 gcgcgaaccg aggtcgcgcg ccgccgcggc ccgaagctcg tcgatgccgg cggacggtgt
     2161 gtagccgtgg cgcccggcac gaatcgcctt gatcgccgca tcctggatgt tgccaggtgt
     2221 cgggaaatcg ggctgtccga tgcagaacga cacgatgtcg cgcccggccc gcacgagttc
     2281 gttgacttcg gccagcacga cgaacgcgtt ctcggtgccg agcgcctcac cccggcgcga
     2341 aactcgcggg gcggtgtgca cagcgctcac atttcactcg atcgtatcgc gctcacggtt
     2401 cgctcgatcg catcggaatg aatcgatc
//
LOCUS       DASUJG010000382         2469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_354553, whole genome shotgun sequence.
ACCESSION   DASUJG010000382 DASUJG010000000
VERSION     DASUJG010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354553"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /gene="pgk"
                     /locus_tag="VFX38_08135"
     CDS             complement(<1..236)
                     /gene="pgk"
                     /locus_tag="VFX38_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX5314853.1"
                     /translation="MSGLKTLDDFALDGRRVLMRVDFNVPLKDGAIANAARIEAALPG
                     IRRVLASGARLLLMSHLGRPEEGKPDKKYSLEPVA"
     gene            complement(233..1249)
                     /gene="gap"
                     /locus_tag="VFX38_08140"
     CDS             complement(233..1249)
                     /gene="gap"
                     /locus_tag="VFX38_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX5314854.1"
                     /translation="MIKVAINGYGRIGRNILRAVYEEGRRKDLQIVAINDLGDAETNA
                     HLTRHDSVHGRFPGEVRVEGGNLVIDGDPVRVCAERDPTKLPWKELGVDLALECTGLF
                     TKRAQAEQHLAAGAKKVLLSAPAKDKVDATIVYGVNHETLDPEKHTVVSNGSCTTNCL
                     APVAQILHRLAGIEGGIMNTVHAFTNDQNLLDVFHKDLRRARAATMSMIPTSTGAAKA
                     IGLVLPELDGKLDGFAIRVPTPNVSFVDLTVLTARAVTVEEVHGAMREAANGYLEGIL
                     AYNEEPLVSIDFNHNPHSSTYESSLTKAKGRLLKVCSWYDNEWGFSNRMLDTAGAMFG
                     IARR"
     gene            complement(1404..>2469)
                     /locus_tag="VFX38_08145"
     CDS             complement(1404..>2469)
                     /locus_tag="VFX38_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006915002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5314855.1"
                     /translation="KAHPKLATELHRRLEGRLPDDWKDAAQAYIEKVAAEGKDRATRK
                     ASGAALAAWQPLLPELVGGSADLTPSNDTWFPDAKVIKPGNPAGNYLHWGVREFGMTA
                     ALNGMAVHGGIIAYGGTFLTFSDYARNAVRLAALGGYPTILVYTHDSIGLGEDGPTHQ
                     PIEHLASLRAMPGLTLWRPADDVETAVAWQDAIERRDGPTAMAFTRQSVPHFPRSAEQ
                     LGEIRRGGYVLQEPEETAVALIIATGSEVGLAAEAAHALGREGKPTRVVSMPCVENFL
                     AQDLEWQERVLPRSIRARVAVEAGVPMPWYRFVGDAGRIVGIERFGESAPGKEVFDEL
                     GFTSAKVATAVRESLAAASK"
BASE COUNT          398 a          796 c          831 g          444 t
ORIGIN      
        1 gcgaccggtt cgagcgaata cttcttgtcg ggcttgccct cttccggacg cccgagatgc
       61 gacatcagca gcaggcgcgc gccgcttgcg agcacgcgcc ggattccggg gagcgcggcc
      121 tcgatgcgcg ccgcgttcgc gatcgcgccg tccttgagcg gcacattgaa gtccacgcgc
      181 atgagcacgc gcctgccgtc gagcgcgaaa tcgtcgagcg ttttgagccc gctcatcgcc
      241 tcgcgatgcc gaacatcgct cccgccgtat cgagcatgcg gtttgagaat ccccactcgt
      301 tgtcgtacca ggagcagacc ttgagcagcc gccccttcgc cttggtgaga ctcgactcgt
      361 aggtcgagga atgcggattg tgattgaagt cgatggagac gagaggttcc tcgttgtagg
      421 cgagaatgcc ctcgaggtag ccgttcgccg cctcgcgcat cgcgccgtgc acttcctcca
      481 cggtgacggc gcgggcggtg agcacggtga ggtccacgaa ggaaacgttg ggcgtcggca
      541 cgcggatcgc gaagccgtcg agcttgccgt cgagttcggg cagcacgagc ccgatcgcct
      601 tggcggcgcc ggtcgaggtc ggaatcatgc tcatcgtcgc cgcgcgcgcg cggcggagat
      661 ccttgtggaa gacgtcgagc agattctggt cgttggtgaa ggcgtgcacc gtgttcatga
      721 tgccgccctc gatgccggcg aggcgatgca ggatctgtgc gaccggcgcg aggcaattgg
      781 tggtgcagga gccgttggaa acgacggtgt gcttttcggg atcgagggtt tcgtgattca
      841 cgccgtagac gatggtcgcg tccaccttgt ccttcgcggg cgcggacagc agcaccttct
      901 tcgcgccggc cgcgagatgc tgttcggcct gcgcgcgctt cgtgaacagt cccgtgcact
      961 cgagcgcgag gtccacgccg agttccttcc acgggagctt cgtcggatcg cgctcggcgc
     1021 agacgcgaac cggatcgccg tcgatgacca gattcccgcc ctcgacccgc acctcgcccg
     1081 ggaagcgccc gtgcaccgaa tcgtgacggg tgagatgcgc attggtttcg gcgtcgccga
     1141 gatcgttgat ggcgacgatc tgcaagtctt tgcgccgccc ttcttcataa acggcgcgca
     1201 ggatgttgcg cccgatgcgc ccgtagccgt tgattgcaac cttgatcatg aaagcattct
     1261 cctgagccgt taatcagccg tcataccggc gaaggccggt atccagtgcg agatgaatcc
     1321 cggcctgcgg ccgggataca aaaatcgact ggaccccggc tttcgccggg gtgacgagag
     1381 ggtttaatcg gagttccata cgttcatttc gatgccgcgg cgagactttc gcgcaccgcc
     1441 gtggccacct tcgccgaagt aaaacccagt tcgtcgaaga cttccttgcc cggagcggac
     1501 tcgccgaagc gctcgatccc gacgatccgc ccggcatcgc cgacgaagcg ataccagggc
     1561 atgggaacgc cggcctcgac cgcgactcgc gcgcggatcg agcgcggcaa cacccgctcc
     1621 tgccattcga gatcctgcgc gagaaaattc tcgacgcagg gcatcgagac cacgcgggtc
     1681 ggtttccctt cgcgaccgag ggcatgcgcc gcttcggcgg cgagaccgac ttccgagccg
     1741 gtcgcgatga tgagcgcgac cgccgtttct tccggttcct gcagcacgta gccgccgcgg
     1801 cggatttcgc cgagctgctc cgcgcttcgc ggaaaatgcg gtaccgactg gcgcgtgaac
     1861 gccatcgccg tcggcccgtc gcgccgctcg atcgcatcct gccaggctac ggcggtctcg
     1921 acatcgtcgg cggggcgcca aagcgtgagt cccggcatcg cgcgcagtga cgccagatgc
     1981 tcgatcggct gatgcgtcgg cccgtcctcg ccgagcccga tggagtcgtg cgtatagacg
     2041 aggatcgtcg gatagccgcc gagcgcggcg agacgcacgg cgttgcgtgc gtaatcggaa
     2101 aaggtgagaa aggtaccgcc gtaggcgatg atgccgccgt gcacggccat gccgttgagc
     2161 gccgcggtca tgccgaactc gcgcacgccc cagtgaagat aattccccgc ggggttgccg
     2221 ggtttgatca ccttggcgtc gggaaaccag gtgtcgttgg agggcgtgag gtccgccgag
     2281 ccgccgacga gttccggcag cagcggctgc caggcggcga gcgcggcacc ggaagccttg
     2341 cgggttgcgc gatccttgcc ctcggcggcg accttctcga tgtaggcctg cgccgcgtcc
     2401 ttccagtcgt cgggaagccg cccctcgagg cggcgatgga gttcggtcgc gagtttcgga
     2461 tgcgccttc
//
LOCUS       DASUJG010000383         2685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_967526, whole genome shotgun sequence.
ACCESSION   DASUJG010000383 DASUJG010000000
VERSION     DASUJG010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967526"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VFX38_08150"
     CDS             complement(<1..361)
                     /locus_tag="VFX38_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5314856.1"
                     /translation="MLEGKRALVTGASRGIGRAIAVALAGAGAEVVGTATGEPGLDAI
                     AAAFAEAGVSGEGRILDLADPASVEAMGEALADRMPDILVNNAGITRDGLLLRMKEED
                     WDAVLATDLTGVFRLTRR"
     gene            complement(385..1329)
                     /gene="fabD"
                     /locus_tag="VFX38_08155"
     CDS             complement(385..1329)
                     /gene="fabD"
                     /locus_tag="VFX38_08155"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017788074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX5314857.1"
                     /translation="MTFAAVFPGQGSQSVGMLAALGQRHSEIGATFAEASELLGYDLA
                     RLVAEGPVEELNRTAITQPALLAAGIGVWRSWLAEGGPQPAVLAGHSLGEYAALVAAD
                     AISFASGLRLVRLRGEAMQDAVAEGAGAMAAILGLDAARLHDLCAEAASIGVVTPANF
                     NSPEQTVIAGERAAVERAGELAAKAGAKRVIGLAVSVPSHCALMEPAAKALGAALAEV
                     EIRPPRIPVLNNVDVAAPASAEAIADALVRQLTQPVRWTEIVRACRDNYGIVALAEFG
                     PGKVLSGLARRIDRSLAALPLGEPAEFEVALQQLTAAA"
     gene            complement(1363..1989)
                     /gene="rrtA"
                     /locus_tag="VFX38_08160"
     CDS             complement(1363..1989)
                     /gene="rrtA"
                     /locus_tag="VFX38_08160"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhombosortase"
                     /protein_id="HEX5314858.1"
                     /translation="MSRWLAMRRTLFRAWPLGLVIVICVALQAGGAAPRLALRYQREA
                     IAAGQWWRLFTGNFVHVGWTHLWLDLVGLLLLWILVGARLSGWRWLFATIAGGWAIGL
                     GLWWAWPAVDWYVGISGIAHTYWAAGALLLLARREWEGWVLLALLGGKLAWEQASGTG
                     LPTSDLLLHAPILTAAHLIGAIAGVAVAAALLLARLRRLRGDAAGLGS"
     gene            2116..2610
                     /gene="gspG"
                     /locus_tag="VFX38_08165"
     CDS             2116..2610
                     /gene="gspG"
                     /locus_tag="VFX38_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEX5314859.1"
                     /translation="MSYLSRRRRAFPPERGFTLIEVMIVVVILAILAAIIVPRVMSAP
                     EKARVTRAKTDIQAVVSALSMYKLDNYTYPTTEQGLAALVTKPASPPVPPNWHQYLSK
                     IPKDPWGQPYKYLFPGTHGEPFDVWTDGPPSNNPGVTDPSAQIGNWNIDEDSSGSGDD
                     NSGG"
BASE COUNT          473 a          949 c          878 g          385 t
ORIGIN      
        1 accggcgggt caggcggaat acgccggtca gatcggtcgc gagcacggcg tcccagtcct
       61 cttccttcat ccgcaggagc aggccgtcgc gggtgatgcc ggcgttgttg acgagaatgt
      121 cgggcatgcg atccgcaagc gcctcgccca tggcttcgac cgaggcggga tcggccagat
      181 cgagaatccg gccctcgccc gacactccgg cttcggcgaa agccgcggcg atggcgtcga
      241 gtccgggctc gcccgttgcg gtgccgacga cctcggcgcc ggcgcccgcg agtgctacgg
      301 cgatcgcccg cccgatccct cgcgaagcgc ccgtaacgag cgcccgtttt ccttccagca
      361 tgaacgcctc cttcaacaaa tgcttcaggc ggccgccgtc aactgctgca aagcgacctc
      421 gaattccgcc ggttcgccga gcggcaacgc agcgagcgat cggtcgatgc ggcgcgcgag
      481 tccgctcaga acctttcccg gcccgaactc cgccagggcg acgatcccgt aattgtcgcg
      541 gcaggcgcgc acgatttccg tccaccgcac cggctgcgtg agctgccgta ccagggcatc
      601 ggcaatcgcc tccgccgatg cgggcgccgc aacgtccacg ttgttgagca ccgggatgcg
      661 aggcgggcgg atttccactt cggcgagggc tgcgcccaat gccttcgcgg ccggttccat
      721 cagtgcgcaa tgcgaaggca cgctgacggc gagcccgatg acacgtttgg ctccggcctt
      781 tgccgccagc tcccccgccc gctcgaccgc cgcgcgttcg ccggcgatca cggtctgttc
      841 gggcgaattg aaattggcag gcgtgacgac tccgatgctc gctgcctcgg cgcaaaggtc
      901 gtgcaggcgg gcggcatcga gtccgaggat cgccgccatc gcgcccgcgc cctccgccac
      961 cgcgtcttgc atcgcctcgc cgcgaaggcg cacgagccgc aggccggagg caaaggaaat
     1021 cgcatctgcc gccaccagcg ccgcatattc tccgaggctg tgaccggcga gcaccgccgg
     1081 ttgaggaccg ccttcggcga gccaggaacg ccatacgccg atgccggcgg cgagcagcgc
     1141 cggctgcgtg attgccgtgc ggttcagttc ctcgaccgga ccttcggcga cgagccgggc
     1201 gagatcgtag cccagcaact cggaggcttc ggcaaacgtc gcgccgatct cggaatgtcg
     1261 ttggccgagc gcggcgagca tcccgacgga ttgggagccc tgcccgggaa agacggcggc
     1321 aaaggtcatg gacaaattct ccgggccggt gcggccttca ttttagcttc ccaaaccggc
     1381 cgcatcgccg cgcagccgcc ggaggcgagc gagcagcagc gccgcggcga ccgcgacgcc
     1441 ggcgatcgcg ccgatgagat gcgccgccgt gagaatcggc gcgtggagga gcagatcgct
     1501 cgtcgggagg cccgttccgc tcgcctgctc ccaagcgagc ttgccgccga gaagcgcgag
     1561 cagaacccag ccctcccact cgcgccgtgc gaggagcagc agcgccccgg ccgcccagta
     1621 ggtgtgggcg attccggaaa ttccgacgta ccagtcgacc gcgggccagg cccaccagag
     1681 accgaggccg atcgcccagc cgcccgcgat cgtcgcgaac agccagcgcc agccggaaag
     1741 gcgggcgccc acgagaatcc acaaaagtag caggccgacg agatcgagcc acagatgcgt
     1801 ccagccgaca tgcacgaagt tgccggtgaa cagccgccac cactgccccg ccgcgatcgc
     1861 ctcgcgctgg tagcgcagcg cgaggcgcgg cgccgcaccg cccgcctgca gggcgacgca
     1921 gatcacgatg accagcccca gcggccaggc ccgaaacagg gtgcggcgca tcgccaacca
     1981 gcggctcatg ggcaacgcca tgtggtgaac cgtcggcgct tcccgcggtc gaatctcgca
     2041 tttccggccg caaacaggcc gcaagagcgt acacttgtcc gcggattcaa tctagcctgc
     2101 cgagggtcga gcagcatgtc gtatttgtcc cgccgacgcc gcgcattccc gccggaacgc
     2161 ggcttcacct tgatcgaagt catgatcgtg gtcgtgatcc tcgcgatcct ggccgccatc
     2221 atcgtgcccc gcgtgatgag cgccccggag aaggcccgcg tcacccgcgc caaaaccgat
     2281 attcaggcgg tcgtctccgc cttgagcatg tacaagctcg acaactatac ctatccgacg
     2341 accgaacagg ggctggccgc actcgtgacc aagccggcga gcccgccggt gccgccgaac
     2401 tggcaccagt atctgtccaa gattccgaag gatccctggg ggcagccgta caaatatctc
     2461 tttcccggaa cgcatggcga gcccttcgat gtttggacgg acgggccgcc gagcaacaat
     2521 cccggggtga ccgatccgag cgcgcagatc ggcaactgga acatcgacga ggacagcagc
     2581 ggcagcggcg atgacaattc gggcggatag cggccgggcg cggcaagcgg cgggattcac
     2641 gctcgttgaa attctcgccg tggtggtgat catcggcatc gtgac
//
LOCUS       DASUJG010000384         2617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1578586, whole genome shotgun sequence.
ACCESSION   DASUJG010000384 DASUJG010000000
VERSION     DASUJG010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1578586"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..309
                     /locus_tag="VFX38_08170"
     CDS             115..309
                     /locus_tag="VFX38_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012267987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX5314860.1"
                     /translation="MRYRIALHQSKEGFSVSVPGLPGCWSQGATEAEAIENIKNAIHE
                     YLAVVEKQLEGETIQEVEVD"
     gene            310..522
                     /locus_tag="VFX38_08175"
     CDS             310..522
                     /locus_tag="VFX38_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012593592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX5314861.1"
                     /translation="MPNIPGINHLDAIRALEKAGLRIMRQGKHIVMSDGTRILTVPRH
                     NPVNSYTLGGIVRDAGLTVEEFRTFL"
     gene            519..1880
                     /locus_tag="VFX38_08180"
     CDS             519..1880
                     /locus_tag="VFX38_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5314862.1"
                     /translation="MNIGALSKATILIADDQADVREALGLLLTGEGYAVETAASPAEV
                     RAALRRRRYDAMFLDLNFQADTTSGREGLTLLTDARERDPDLPIIVLTGWASTEIVVA
                     AMQAGAEDFLEKPWENARVLTVLKNQIALAAARADNRRLLAEVENSDEGGIVFRSAAM
                     REMMATVAKIASADVSVLLTGESGTGKGLLARTVHERSRRAAGPFVHVNLGALPETLA
                     ESELFGHVRGAFTDARADKPGRFELADGGTLFLDEISNATGAMQARLLTALESGSVER
                     LGATRARNVDVRIVSASNADLGAMIARDAFRNDLYFRLNTVEIRIPSLRERPEDIPVL
                     AAHFLTRFARHHGKSLEGFARTAEAALLGYDWPGNVRELAHVIERAVLLAAGARIEAG
                     DLRLPGTAGEATTFEAMSLEQAERYLLQKALARNDGDAAAAARQLGVSRSAFYRRLAK
                     LKQ"
     gene            2026..>2617
                     /locus_tag="VFX38_08185"
     CDS             2026..>2617
                     /locus_tag="VFX38_08185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5314863.1"
                     /translation="MKRPAHEWRLALLALVGLVPALGLFAAAPWLGLALWAHALLVAG
                     AALAGLAAAFAIARRSRRASRRLASLVLGIRDSEFGLRARVEAGAFGETLAALNALAE
                     ELAGMRRAGIESDALLGKLLSAVELAILVFDPRGRLVGVNRAGETLFGAGADALYGRS
                     ATTLEVADWLAADAPLRATKTLSAGDGPWEVRVARFR"
BASE COUNT          440 a          825 c          874 g          478 t
ORIGIN      
        1 cagacgagat tgttgcggcg gctgaaggcg caatcagcaa gcggtgattg tatcaggcga
       61 cgtgaaaatc tttacgatcg gtaggcactg agttcccttg gaaaggagga ttttatgcgt
      121 tacaggatag ctttgcatca gtcgaaggaa ggattctcgg tttcggtgcc ggggctgccg
      181 ggatgctggt cgcagggcgc taccgaggcc gaggcgattg agaatatcaa gaacgccatt
      241 cacgaatatc tcgcggttgt cgaaaagcag cttgagggcg agacgattca ggaagttgag
      301 gttgattgag tgcctaacat acccggcatc aaccatctcg atgccatacg cgccttggag
      361 aaagcgggat tgagaatcat gcgtcagggc aagcacatcg tcatgtcgga tggaacgcgc
      421 atcctgacgg ttccgaggca taatccggtc aattcctaca cgctgggcgg tatcgttcgc
      481 gacgcagggt tgaccgtcga ggaatttcgc acgtttttgt gaacataggt gctctcagca
      541 aggcaacgat tttgattgca gacgatcagg ccgacgtgcg cgaggcgctc ggcctgcttc
      601 tgactggcga gggatatgcg gtagaaacgg cggcttcgcc ggcggaagtg cgcgccgccc
      661 tgcgccggcg gcgctacgac gccatgttcc tcgacctcaa ctttcaggcg gacacgacct
      721 cggggcgcga gggtttgacc ttgctcaccg atgcgcgcga acgcgatccc gatcttccga
      781 tcatcgtgct taccggctgg gcgagcacgg aaatcgtggt tgcggcgatg caggcgggag
      841 ccgaggattt tctggagaag ccctgggaga acgcgcgcgt tctcacggtt ctcaagaatc
      901 agatcgccct cgccgctgcc cgcgcggaca accggcgtct gctcgccgaa gtcgaaaact
      961 ccgacgaagg cggaatcgtc tttcgttcgg cggcgatgcg cgagatgatg gcgacggtag
     1021 caaagattgc ctccgccgat gtctctgttc tgctcaccgg cgagagcggt accggcaagg
     1081 gactgctcgc gcgcaccgtg cacgaacggt cgcggcgcgc cgccggcccc ttcgtgcacg
     1141 taaatctcgg cgccctgccg gaaacgctcg ccgaaagcga actcttcggt cacgtcaggg
     1201 gcgcgttcac cgacgcgcga gccgacaagc ccgggcgctt cgagctcgcc gacgggggaa
     1261 ctctttttct cgacgagatt tcgaacgcga cgggcgccat gcaggcgcgg ctgctgacgg
     1321 cactcgaaag cggatcggtc gaacggctcg gcgccacgcg cgcgcgcaac gtcgacgtgc
     1381 gcatcgtttc cgcgagcaat gccgatctcg gcgcgatgat cgcgcgggat gcatttcgca
     1441 acgacttgta ttttcggctg aatacggtgg agatccgcat cccctccctg cgcgaacggc
     1501 cggaggatat cccggtcctc gcggcgcatt tcctcactcg cttcgcgcgc catcacggga
     1561 agtcgctcga aggtttcgcc cgcactgcgg aggcggcgct gctcggctac gactggcccg
     1621 gaaacgtgcg cgaactcgcg catgtcatcg aacgcgcggt gctgctcgcg gccggcgcgc
     1681 gaatcgaagc gggcgatctc aggttgccgg gaacggcggg tgaagcgacg acgttcgagg
     1741 cgatgagtct cgaacaggcc gaacgctatc ttctgcaaaa ggcgctcgcg cgcaacgacg
     1801 gcgatgcggc cgcggcggcg cgccagctcg gcgtctcgcg cagcgccttt tatcgccgac
     1861 tcgccaaact caagcaatga gggcttgctt ccggcactat tccgcgcagg tcatgccggt
     1921 gagggccggc atgcagtcga caatgattcc cgccggttgg gcgcgatgcg ttttcctgcc
     1981 gagtcccggc cttcgccgcg acgacgatac gcagcgtgct gccgcatgaa aagaccggcg
     2041 cacgaatggc ggcttgcgct cctcgcgctc gtcggtctcg ttccggctct cggcctcttc
     2101 gccgccgcgc cctggctcgg tctcgctctc tgggcgcacg ccctgctcgt cgcgggcgcc
     2161 gcgcttgcgg ggcttgccgc cgccttcgcg atcgcccggc gttcgcgccg cgcttcgcgc
     2221 cggctcgcga gtctcgtgct cggaatccgc gacagcgaat tcgggctgcg cgcccgcgtc
     2281 gaggccggtg ccttcggcga aacgctcgcg gcgctcaacg cgctcgccga ggaactcgcg
     2341 ggcatgcgcc gtgcgggcat cgaatcggat gcgctcctcg gaaagctgct ttctgcggtg
     2401 gaactcgcca tcctcgtgtt cgatccgcgc gggcgtctgg tcggagtcaa tcgtgccggc
     2461 gaaaccctgt tcggggccgg cgccgatgcg ctctacggac gcagcgccac gacgcttgaa
     2521 gtcgccgact ggctcgcggc ggatgctccg ctgcgcgcaa cgaaaacctt gtccgcgggc
     2581 gacggcccct gggaggtgcg ggtcgcgcgc tttcgcc
//
LOCUS       DASUJG010000385         2296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_478636, whole genome shotgun sequence.
ACCESSION   DASUJG010000385 DASUJG010000000
VERSION     DASUJG010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478636"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="VFX38_08190"
     CDS             complement(<1..912)
                     /locus_tag="VFX38_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314864.1"
                     /translation="MTRHHRNNAPKTAPAAPPLSPPAWYERREIWWLAIAVLLGVTWL
                     VYMRGLTGGWLLDDYGNIVNNDALKLPRLAWAPLWHAMWSFDAGPLGRPLSLAGFALQ
                     RYFVGLDPYSFKAVGLGMHMLAALLFAGFTRALLRAWRVRRAPALSLARCDWTAFAIG
                     AAWALHPFNLTPVLYAVQRETVQAALFTIGGLWAYVSMRERLRPSGPHLLLLAVVLGV
                     FTFLGAFSKETGLLLPLFTFLLEVFVFGFRDRTGTRWRMATFYVIVLLIPTVLGLWWM
                     LPGVFSPHAYATREFDLNQRILTEGRVM"
     gene            complement(909..>2296)
                     /locus_tag="VFX38_08195"
     CDS             complement(909..>2296)
                     /locus_tag="VFX38_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314865.1"
                     /translation="RLLAGETGSGLALFAAALVATALATFTKEDGALTLLYAFVIEWT
                     IFAFRRGDGRRSRLTIAFFVVFLAFPAAGAVAWLLPGTLHPSAWASRSFGLGERILSE
                     GRVIWDYFVWSLFPDLRQLTLYHDGFAASRGLFTPWTTLLAWAGIAVFLAAGILLRRR
                     RPLVSLGILWFLAGQVLTASFIPLELVFEHREYLPSLGLYLALFAPIALADPRVRLLR
                     ARAAALAAIVLLYGGALTLRSLDWSNPLLQAAIAAKAHPDSPRATYGYGRTLAMFATL
                     DPDIVPRARAALETASRVPGQTTLPDSALILLAHNSGEPMRREWFADMEKQLRRRAPG
                     PEDIAALDSLVHCAIREKNPCRFPPYALDSVFASALTGRVPNGYLMAIYGNYLLNARR
                     NPGAAAGIFRALVHLSPRTPAYHFNLGVCEANLDHLALARAELATLKRLNRFGLNDSQ
                     VEALTRLIAHP"
BASE COUNT          435 a          699 c          880 g          282 t
ORIGIN      
        1 catcacccgc ccttcggtga ggatgcgctg gttgagatcg aactcgcggg tcgcgtaggc
       61 gtgcggcgaa aacactcccg gaagcatcca ccagagtccg agcaccgtcg ggatgaggag
      121 cacgatgacg tagaaggtcg ccatgcgcca gcgcgtgccg gtacggtcgc ggaatccgaa
      181 gacgaagact tccagcagga aggtgaagag cggcagcagc agcccggttt ccttggagaa
      241 ggcgccgaga aaggtgaaga ctccgagcac gacggcgagc aggaggagat gcggcccgct
      301 cgggcgcagc cgttcgcgca tggatacgta ggcccacagc ccgccgatgg tgaagagcgc
      361 ggcctgcacc gtctcgcgct gcaccgcgta gaggacgggc gtcaggttga agggatgcag
      421 cgcccaggcg gcgccgatcg cgaacgccgt ccagtcgcag cgcgcgaggc tcagcgccgg
      481 cgcgcggcgc acgcgccagg cacgaagcag cgcgcgggtg aagccggcga agagcagggc
      541 cgcgagcatg tgcatgccga gccccaccgc cttgaagctg tagggatcga ggccgacgaa
      601 atagcgttgc agcgcaaagc ccgcgagcga cagcgggcgg ccgagcggtc ccgcgtcgaa
      661 ggaccacatc gcatgccaca gcggcgccca ggcgagacgc gggagcttga gcgcatcgtt
      721 gttgacgatg ttgccgtagt cgtcgagcag ccagccgccg gtgagcccgc gcatgtacac
      781 gagccaggtc acgccgagga gcaccgcgat cgcgagccac cagatttcgc gccgctcgta
      841 ccaggcgggc ggggaaagcg gcggcgcggc cggggcggtt ttgggagcgt tgttgcggtg
      901 gtgtcgcgtc atggatgggc gatcagacgg gtgagggcct cgacctggga gtcgttcagg
      961 ccgaagcgat tgagtcgttt cagggtcgcg agttccgcgc gtgcgagcgc gagatgatcg
     1021 aggttggcct cgcagactcc cagattgaag tggtaggccg gcgtgcgggg cgacaagtgt
     1081 accagcgccc ggaagattcc ggcggcggcg cccgggttgc gccgcgcgtt gagaaggtag
     1141 ttgccgtaga tggccatgag gtagccgttc ggcacccggc cggtgagcgc ggaggcgaag
     1201 accgaatcga gcgcataggg ggggaagcgg cagggatttt tctcgcggat cgcgcagtgc
     1261 acgaggctgt cgagggcggc gatgtcctcg ggaccgggcg cgcgccgccg caactgcttc
     1321 tccatgtccg caaaccattc gcggcgcatc ggctcgccgc tgttgtgggc gagaaggatg
     1381 agcgccgaat ccggcagagt ggtttgtccc ggcacgcggc tcgccgtttc cagcgcggcg
     1441 cgggcgcggg ggacgatgtc cggatcgagc gtcgcgaaca tcgcgagcgt gcgcccgtag
     1501 ccgtaggtcg cccgcggcga gtcgggatgc gccttcgccg cgatcgcggc ctgcagcagc
     1561 ggattcgacc agtcgagcga gcgcagcgtg agcgccccgc cgtacagaag cacgatggcg
     1621 gcgagcgcgg cggcgcgcgc gcgcaggaga cgcacgcgcg gatcggcgag ggcgatcggc
     1681 gcgaagagcg cgagataaag tccgagcgag ggcagatatt cgcgatgctc gaagacgagt
     1741 tcgaggggaa tgaaacttgc cgtgagcacc tgcccggcga gaaaccagag gatgccgaga
     1801 ctcacgagcg gtcggcggcg gcgcagcagg attccggccg caagaaacac ggcgatcccc
     1861 gcccaggcca gcagcgtcgt ccagggcgtg aagagtccgc gcgaagccgc gaagccgtcg
     1921 tgataaagcg tgagctggcg aagatcggga aagagcgacc agacaaagta atcccagatc
     1981 acccgccctt cgctcaagat gcgctcgccg agcccgaagg agcgcgaagc ccaggcggac
     2041 ggatgcagcg ttcccggcag cagccaggcg acggcgcccg cggcagggaa cgcgagaaag
     2101 acgacgaaga aggcgatcgt gagccgcgag cggcgcccgt cgccgcggcg gaaggcgaag
     2161 atcgtccact cgatgacgaa ggcgtagagg agcgtgagcg cgccgtcttc cttggtgaag
     2221 gtcgcgagcg cggtggcgac gagcgccgcg gcgaagagcg cgaggccgct ccccgtttcc
     2281 cccgcgagca gccgcc
//
LOCUS       DASUJG010000386         2547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1090100, whole genome shotgun sequence.
ACCESSION   DASUJG010000386 DASUJG010000000
VERSION     DASUJG010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090100"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="VFX38_08200"
     CDS             <1..967
                     /locus_tag="VFX38_08200"
                     /inference="COORDINATES: protein motif:HMM:NF024045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX5314866.1"
                     /translation="GRLDLAQAEAVADLIEAGSAAAAKAALASLEGRFSREIQALAAE
                     LLRLRVEIEARLDFPDDEIDRAGTLAALHALQERLRKVGEAAASGRRLAEGFVCVLAG
                     APNVGKSSLLNALAGSERAIVTELPGTTRDPIEVDITLDGLALRLVDTAGLRDSDDRI
                     EAEGVRRARERAAGADLVLEVRDDRVPMPALSRASGPRLLVINKIDLSGRSPGSFRLA
                     GERAVAVSASTGAGLEDLAACLREHALGGSFDEAAGFSARRRHLDALSRVSNAVSAAA
                     AALASGDEVAAEELRRAQSALGEITGEVTTEDLLGAIFSSFCLGK"
     gene            1051..1458
                     /locus_tag="VFX38_08205"
     CDS             1051..1458
                     /locus_tag="VFX38_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314867.1"
                     /translation="MSKRSSLERLLRRGLLVLLIALAAAAVYLWVYRGSGEAQAFDRI
                     YEASVAQASGNLRLEAALGVPIQASEQAASHKFYREDQRHHVRFHYPLSGPRGTVSIE
                     GEAIQLGRNWLIVALQARFPNDATLDLTSNVAT"
     gene            1455..2486
                     /gene="hemB"
                     /locus_tag="VFX38_08210"
     CDS             1455..2486
                     /gene="hemB"
                     /locus_tag="VFX38_08210"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX5314868.1"
                     /translation="MSPRGAFPGSYPRRRPRRLREKAFVRELVREHRLAPSDFVWPVF
                     VCEGEARREPVASMPGVERLSVDLLIEEARAAATLGIPAIALFPVVPPAKKTSGGDEA
                     CNPDGLIPHAVAALKSALPELGVICDVALDPYTSHGQDGVLDERGYIANDETVAVLVK
                     QALVQAAAGADVVAPSDMMDGRIGAIRDALEAAGHVNTRILAYSAKYASAFYGPFRDA
                     AGSAANLGAGGKETYQMDPANGDEALHEVAMDLEEGADMVMVKPGLPYLDILRRVKEA
                     FGVPTFVYQVSGEYAMLKAAAANGWLDEKKTVLETLIACKRAGADAILTYYARDAARW
                     LEQEAGGVP"
BASE COUNT          417 a          833 c          880 g          417 t
ORIGIN      
        1 cggcaggctc gatctcgcgc aggcggaagc ggtcgcggac ctgatcgaag cgggatccgc
       61 ggccgcggca aaggcggcgc tggcgtccct cgaagggcga ttctcgcggg aaattcaggc
      121 cctggccgcc gaactcctgc gcctgcgggt ggaaatcgag gcaaggctcg attttccgga
      181 cgacgaaatt gaccgcgccg gaactctcgc cgccctgcac gccttgcagg agcggctgcg
      241 gaaagtcggc gaagcggccg cgagcggccg acgcctcgcc gaaggcttcg tctgcgtgct
      301 cgcgggcgcc ccgaacgtcg gcaaatcctc gcttctcaac gccctcgccg gaagcgaacg
      361 cgcgatcgtc accgaactgc cgggcacgac ccgtgacccg atcgaggtgg acatcacgct
      421 cgacgggctg gcgttgcggc tcgtggacac ggccggactg cgcgactccg acgaccggat
      481 cgaggcggag ggagtacgcc gggcccgcga acgtgcggcc ggcgccgatc tggtgctcga
      541 ggtgcgcgac gatcgcgtgc cgatgccggc attgtctcgc gcctccggcc cgcgactgct
      601 ggtgatcaac aagatcgact tgagcgggcg ttcgcccggt tcgttccgtc tggcgggaga
      661 gcgcgccgtc gcagtctcgg cgagcacggg agcggggctc gaggatcttg cggcctgtct
      721 gcgcgaacac gccctgggcg ggagtttcga cgaagccgca ggattttccg cgcggcgccg
      781 acatctcgat gcgctcagtc gggtttcgaa cgccgtttcg gctgcggcag ccgccctggc
      841 ctctggcgac gaagtggccg ccgaggaact gcgccgcgcc cagagcgcgc tcggagaaat
      901 taccggcgag gtaaccaccg aagacctcct cggcgccatc ttttcgagct tctgcctcgg
      961 caaatgatga cggcgggggc gtcgtccgcg ttcctttcca aacgttcgcc tgaagcctta
     1021 cactgacgac gggcgcaggg aacattgcaa atgagcaaga ggtcgtcgct ggagcggctg
     1081 ctgcgccgcg ggctgctcgt cctgctcatc gcgctcgcgg ccgcggccgt gtatctgtgg
     1141 gtctatcgag gcagcggcga ggcgcaggcc ttcgatcgga tctacgaggc gagcgtggcg
     1201 caggcgagcg gcaacctgcg tctcgaggcc gcactcggag ttccgattca ggcttcggag
     1261 caggccgcga gccacaagtt ttaccgcgag gatcaacgtc accatgtccg tttccactat
     1321 ccgcttagcg gaccgcgggg caccgtgagc atcgaaggcg aggcgatcca gttgggtcgc
     1381 aactggttga tcgtggcctt gcaggcgcgc tttcccaacg atgcgacgct tgatctcacc
     1441 tccaacgtgg cgacatgagc ccgcgcggcg cattcccggg aagctatccg cgtcgccggc
     1501 cgcggcggct tcgcgaaaag gccttcgtac gcgaactcgt gcgcgaacat cgcctcgcgc
     1561 cttcggactt cgtctggccg gtgttcgtct gcgagggcga agcgcgacgg gaaccggtcg
     1621 cttccatgcc gggcgtggaa cgcctcagtg tggatttgct gatcgaggaa gcgcgtgccg
     1681 cggcgacgct cggcattccc gcgatcgcgc tatttcccgt agttccgccc gcgaagaaaa
     1741 cctcgggcgg cgacgaggcc tgcaatcccg acgggttgat tccgcatgcc gtcgcggcgc
     1801 tgaagtctgc tttgcctgaa ctcggcgtga tctgcgacgt ggcgctcgat ccctacacca
     1861 gccacgggca ggacggagtg ctcgatgagc gcgggtatat cgcgaacgac gaaaccgtcg
     1921 cggttctggt gaaacaagcg ctcgtgcagg ccgcggccgg agccgacgtg gtcgcgccct
     1981 cggacatgat ggacgggcgc atcggcgcga tccgcgatgc gctcgaagcg gcgggccacg
     2041 tcaatacgcg cattctcgcc tacagtgcga agtatgcctc ggcgttctac ggaccgttcc
     2101 gcgacgccgc cggttcggcc gcgaatctgg gtgccggcgg caaggaaacc taccagatgg
     2161 atcccgccaa cggcgacgag gccctccacg aagtcgcgat ggatctcgaa gaaggcgccg
     2221 acatggtgat ggtgaagccg ggcctgccgt acctcgacat cctgcgccgg gtgaaggaag
     2281 ccttcggcgt gccgaccttc gtctatcagg tgagcggcga gtacgccatg ctcaaggccg
     2341 cggcggcgaa cggctggttg gacgaaaaga agaccgtgct cgaaacgctc atcgcctgca
     2401 agcgcgccgg cgccgacgcg atcctgacct actacgcccg cgacgctgcc cgctggctgg
     2461 agcaggaggc gggaggcgtt ccctgagccg agcccagccg gttggcacgg ccgagtatcg
     2521 ccaattgctt tcctgattcg atcttcg
//
LOCUS       DASUJG010000387         4789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1701028, whole genome shotgun sequence.
ACCESSION   DASUJG010000387 DASUJG010000000
VERSION     DASUJG010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1701028"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="VFX38_08215"
     CDS             <1..1031
                     /locus_tag="VFX38_08215"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEX5314869.1"
                     /translation="LRPDDVLWVHDYHLVPLASEIRARGFEGPIGFFLHTPFPPLDIL
                     RSLPPHRDFLNWFGAYDLVGFQTEVDRRAFLGAMRFDLGATADRRGDIKLGARRIASR
                     VVPIGVDVTEVAAQARHGRSSRHVAMLKTSLEGRQLIIGADRLDYSKGLIERFRAYQH
                     LLATHPRLLGDIVYLQIAEPSRSEVPEYQKVRHELDQVAGEIIGHYARFDWMPMRYLN
                     RRMQRPTLLAFLSVARVGLVTPLRDGMNLVAKEFIAAQDPEEPGVLVLSEMAGAAAQL
                     PAALLVNPYDVEGVGETIAEALAMPLAERRERWQSLFASVRRDDIRAWSERFLEALAE
                     AYASRKSS"
     gene            1028..1999
                     /gene="glk"
                     /locus_tag="VFX38_08220"
     CDS             1028..1999
                     /gene="glk"
                     /locus_tag="VFX38_08220"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEX5314870.1"
                     /translation="MNNLIGDIGGTNTRLALAAAGEPRWDRLETYRNDDYESLAAVIE
                     EYLGADGSACEAAALAVAGPVRRGVAKLTNRDWTISASTLRERFRWKHCTVVNDFSAV
                     ALAIPALRATDLEQTGGGERDAEEPVAILGPGTGLGVAGLIPGADGGRVLVTEGGHAT
                     ATAIGERSAAICDRLRRRFGHVSIERIVSGQGMENIYRAIGELDGKNVEARSAAEIGE
                     AAMQGDDPVAAETMEQFFALLGAAAGNLALTYGAFGGVYIAGGIVPRYLPLFRCSRFR
                     ESFEAKGRLSGYVRAIPAFVILHEEVELLGLAASLAARGAGRAWPRV"
     gene            1996..2940
                     /locus_tag="VFX38_08225"
     CDS             1996..2940
                     /locus_tag="VFX38_08225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5314871.1"
                     /translation="MSAAGGGFEARHRIGILVLNFNGAELTRACVASILAHTSPSLDF
                     AILVVDNGSDAGELERLASLPQSERLRLVASRINLGFGGGHAFGLQFLWADHYLFLNS
                     DCVFRNDVVAALAEFFDRNPRAGLASGLSFDSDGNFRANYHPAPNLGELLVGRALARR
                     FAPARFPDRRQEPREPLAVEVAGGAALYVRAEAFFAVGGFDPFFFLYCEEEDLALRLR
                     RAGWGVWIVPGGRFFHAGGGSTPKDPAYRREFFISFLHYLRKHRSAAAVLAFRVIYAL
                     KLLRRAGRDAACARLAWFVLCGANPAASLRFRQRPREL"
     gene            complement(2951..3850)
                     /locus_tag="VFX38_08230"
     CDS             complement(2951..3850)
                     /locus_tag="VFX38_08230"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX5314872.1"
                     /translation="MTGAIAASEKSATAARDELSSLLRDAFVLGREPALKLALEGDDP
                     ATAARLLAYYGYYSRAQTAQIAALTAELAQYRSLQSRLADTGRELENTVATRKSSLAD
                     LEQSRDRRKALVARLASDISRTHAQADSLRADAARLEKIVRSVRRDIAAAPPPPVPAG
                     VAFGKLRGRLPWPVAGKIVDRYGSARADGELEWRAVRIAAPGGTRVHAIAYGRVVYAG
                     WLPYYGLVLMIDHGDGFLAVYGHNEALYKQVGDRVRPGDVITTVGVSGGQPRPMLYFQ
                     LRHDDQPLDPSQWCVAGGPPRRP"
     gene            3959..4216
                     /locus_tag="VFX38_08235"
     CDS             3959..4216
                     /locus_tag="VFX38_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314873.1"
                     /translation="MAAGDFLMLGGIERGNLLLETLQLRRVILPVGGAPCRGRERDCE
                     QQRDRRREAGATAKAGNQARDTRSVETTTQLPGLPLNCRYE"
     gene            complement(4197..>4789)
                     /locus_tag="VFX38_08240"
     CDS             complement(4197..>4789)
                     /locus_tag="VFX38_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX5314874.1"
                     /translation="PRRFGTGENVMAARQKPKKRKPVRRGGTAPIWFVAGALLAVLII
                     FGYPAVRNAVQHRSTGVAQQGGAPAGGRQENPAAAATTGESFDFYRMLSHPTQILTSG
                     ETAEVQPAKPAQSVAEPGSYILQVASFRAAKDAEALKAQLALWGVSAEVQAVTVQGET
                     WHRVRIGPVSDLASLNAMRSRLAAHKLKPLLIRTGN"
BASE COUNT          706 a         1632 c         1614 g          837 t
ORIGIN      
        1 tgcttcgccc cgacgatgtt ctgtgggtgc acgactatca tcttgtgcct ctcgcctccg
       61 agatacgcgc ccgaggtttc gaggggccga tcggcttttt tctccacacg ccgttcccgc
      121 cgctcgatat cctgcgctcc ctgccgcctc atcgcgactt tctcaactgg ttcggggcct
      181 acgatctggt cggcttccag acggaagtgg accggcgcgc gtttctcggc gcgatgcgat
      241 tcgatctcgg tgcgactgcg gatcggcgcg gcgacatcaa actcggcgcg cggcggatcg
      301 cgagccgcgt ggttccgatc ggcgtggacg ttaccgaagt cgcggcccag gcgcgccatg
      361 gccggagttc gcgccatgtc gcgatgctca agacgagcct cgagggccgg cagctcatca
      421 tcggcgcgga tcgcctcgat tattccaagg gcttgatcga acgctttcgc gcctatcagc
      481 acctgctcgc gacgcatccg cggctgctcg gcgacatcgt ctacttgcag atcgcggagc
      541 cgtcgcgctc cgaggtgccg gaataccaga aggtacgtca cgaactggat caggtggcgg
      601 gcgagatcat cggccactat gcccgcttcg actggatgcc gatgcgctat ctcaaccgcc
      661 ggatgcaacg gcccacgctg cttgcgtttc tctcggtcgc gcgggtggga ctcgtcacgc
      721 cgcttcgcga cggcatgaac ctcgtcgcca aggagttcat cgccgcgcag gacccggagg
      781 agcccggcgt actcgtgctc tccgagatgg ccggggcggc ggcgcagctt ccggccgcgc
      841 tcctcgtcaa cccctacgac gtcgagggag tgggcgagac catcgccgag gcgctcgcga
      901 tgccgctcgc cgaacggcgc gaacgctggc agagcctctt cgcctcggtg cgccgcgacg
      961 atatccgcgc ctggagcgaa cgttttctgg aagcgctggc ggaagcctac gcaagccgca
     1021 agtcttcatg aacaatctga tcggcgacat cggcggtacc aatacccggc tggcacttgc
     1081 cgccgccggc gagccccgct gggaccggct cgaaacctat cgcaacgacg actacgagtc
     1141 gctcgcggcg gtcatcgagg aatacctcgg cgcggacggg tcggcttgcg aagcggcggc
     1201 tcttgccgtg gccggcccgg tgcgccgggg agtcgcgaaa cttaccaatc gcgactggac
     1261 catttcggcg agcacgctga gggagcgatt ccgctggaag cattgcaccg tggtcaacga
     1321 tttttcggcg gtcgcgcttg ccatcccggc gcttcgcgcg acggatctgg agcagaccgg
     1381 aggcggcgag cgcgatgccg aggaaccggt cgcgatcctc gggccgggga ccggtctcgg
     1441 cgtcgccggg ctcatcccgg gagccgacgg cggacgagtg ctcgtcaccg aaggcggaca
     1501 cgcgacggcg acggcgatcg gggagcgcag cgcggcgatc tgcgatcgcc tgcgccggcg
     1561 cttcggtcac gtctcgatcg aacgaatcgt ttcgggccag ggaatggaga atatctatcg
     1621 cgccatcggc gagctggacg gcaagaacgt cgaggcgcgc agcgccgccg agatcggaga
     1681 ggcggcgatg cagggagacg atccagtcgc ggccgagacg atggagcagt tctttgcgct
     1741 gctcggggcc gcggcgggca acctcgccct cacctatgga gccttcggcg gcgtgtacat
     1801 cgcgggcggc atcgtgccgc gctatctgcc gctctttcgc tgctcgcgct ttcgcgaatc
     1861 gttcgaagcc aagggccgac tgagcggcta cgtccgcgcg attcccgcct tcgtcattct
     1921 gcacgaagag gtggaacttc tcgggctcgc ggcgagcctc gctgcccgcg gcgccggccg
     1981 agcctggccg cgggtgtgag cgctgcgggc ggcggcttcg aagctcgcca ccggatcggc
     2041 atcctcgttc tcaacttcaa cggcgccgaa ctcacccgcg cctgcgtcgc ctcgattctc
     2101 gcgcacactt ccccgtcgct cgacttcgcg atactggtcg tggacaacgg ctccgacgcc
     2161 ggcgaactcg aaagattggc atccctgccg caatcggagc gcctgcgtct cgtcgcgagc
     2221 cggatcaatc tcggcttcgg cggcggtcac gccttcggcc tgcaattcct ctgggcggac
     2281 cactacctgt ttctgaacag cgactgtgtt tttcgcaacg acgtcgtggc cgcgcttgcc
     2341 gaatttttcg atcgcaatcc gcgggcgggc ctcgcgagcg gactcagttt cgattccgac
     2401 ggaaacttcc gcgcgaacta tcatccggcg ccgaatctcg gcgagcttct cgtcggacga
     2461 gcgctcgcgc gccgcttcgc tcccgcgcgg ttccccgacc ggcgccagga gccgcgggaa
     2521 ccgctcgcgg tcgaagtcgc gggcggagcg gctctctacg tgcgggcgga ggcgtttttt
     2581 gccgtcggcg gattcgatcc ctttttcttt ctctattgcg aagaggagga tctggccctg
     2641 cgcctgcgtc gcgccggctg gggcgtgtgg atcgtgccgg gggggcgctt cttccatgcc
     2701 ggcggcggca gcacgccgaa ggacccggcg tatcggcgcg agtttttcat ttcgttcctg
     2761 cattacctgc gcaagcatcg ctccgccgcg gccgtgctcg cgttccgcgt gatttatgcg
     2821 ctgaaacttc tgcgccgcgc cgggcgcgac gccgcctgtg cccggctcgc gtggttcgtt
     2881 ttgtgcggcg ccaatccggc ggcgagcctg cggttccggc agcgcccgcg cgaactctga
     2941 gccgggatct tcagggtctc cggggtggac cgccggccac gcaccactgc gaggggtcga
     3001 gcggctggtc gtcgtggcga agctggaaat agagcatcgg tcgcggctgt ccgccgctca
     3061 cgccgaccgt ggtgatgaca tcgcctgggc gcacccgatc gccgacctgc ttgtagagcg
     3121 cctcgttgtg gccgtagacg gcgagaaatc catcgccgtg atcaatcatc aacaccagac
     3181 cgtaataggg cagccagccg gcgtacacca cccgcccgta ggcaatggcg tgcacgcgcg
     3241 tgccccccgg cgccgcgatg cgcaccgccc gccactcgag ttctccgtcg gcccgcgccg
     3301 aaccatagcg atcgacaatc tttccagcga cgggccaggg cagtctgccc cgcagtttgc
     3361 cgaaggcgac gccggcggga acgggaggcg gcggcgccgc cgcgatgtct cgacgcaccg
     3421 agcgcacgat cttctcgagg cgcgccgcat ccgcccgcaa cgaatccgcc tgcgcgtggg
     3481 tgcgggaaat gtcgcttgcg agccgggcga ccaaggcctt gcggcgatcg cggctttgtt
     3541 cgagatccgc gagcgaactc ttgcgcgtcg cgaccgtgtt ctccagctcg cggccggtat
     3601 cggcgagccg gctctgaagg ctgcggtact gcgcaagctc cgccgtcagc gccgcgatct
     3661 gcgccgtctg cgcccgggag taatagccgt agtaggcgag caggcgcgcc gccgtcgcgg
     3721 gatcgtcgcc ttcgagcgcg agtttcagcg ccggttcgcg cccgagaacg aacgcatcgc
     3781 gaagcagcga ggagagctcg tcgcgcgcgg cggtcgcgga tttttcggat gccgcgatcg
     3841 cgcctgtcaa ctccagcttc tgtttcgtga gcgaggcttt tcgtgtgccg agcgctgcca
     3901 gttcccgctc cagcgaaccg atgcgctgct ccagttctcc gagttcgccg agcgccttgt
     3961 ggcggcgggc gatttcctga tgctcggcgg aattgagcgc ggcaatcttc tccttgagac
     4021 gttgcagttg cgccgcgtta tcctgcccgt tggcggcgcc ccatgccgcg ggcgcgagcg
     4081 cgattgcgag cagcagcggg accgtcgccg ggaggcaggc gcgacggcaa aggctgggaa
     4141 tcaggcccgc gacacgcgca gcgtcgagac gacgactcag ctccccggtc tcccgctcaa
     4201 ttgccggtac gaatgagcag cggtttgagc ttgtgcgccg cgagccggct gcgcatggcg
     4261 ttcagcgagg ctaggtcgct caccggcccg atccgcacgc gatgccaggt ttcgccctgc
     4321 accgtcaccg cctgcacctc ggcgctcacg ccccaaagcg cgagttgcgc cttgagcgcc
     4381 tcggcatcct tggccgcgcg gaaggacgca acctgaagaa tgtacgaacc gggttcggca
     4441 accgactgcg ccggcttcgc gggttgaacc tccgccgttt cgcccgaggt gagaatctgg
     4501 gtggggtggg agagcatccg gtagaagtcg aacgattcgc cggtcgtggc cgcggcggcg
     4561 ggattctcct gccgcccgcc cgcaggcgcg ccgccctgct gcgcgactcc cgtactgcga
     4621 tgctgcaccg cattgcgcac ggcgggatag ccgaagatga tgagcaccgc gaggagcgcc
     4681 ccggcaacaa accagatcgg cgccgtcccg ccgcggcgca ccggctttcg cttcttcggt
     4741 ttctgccgag ccgccatcac attttctccg gtgccgaaac gccgaggag
//
LOCUS       DASUJG010000388         5029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_235747, whole genome shotgun sequence.
ACCESSION   DASUJG010000388 DASUJG010000000
VERSION     DASUJG010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235747"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VFX38_08245"
     CDS             complement(<1..478)
                     /locus_tag="VFX38_08245"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF015280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX5314875.1"
                     /translation="MGRRLIVNLGSTRIAWRGAGGSDVRVHDGRPGQVIAGVLAGSEP
                     PPEVIVGSVAAQPVTEEFLAACRANWDLDPCELVATREACGVRNAYAEPARLGIDRWA
                     AMIAAYRAHGGAVLLADCGTAVTVDYVAGEGRHVGGLIAPGIGLMRKALASGTRLAS"
     gene            complement(463..1416)
                     /locus_tag="VFX38_08250"
     CDS             complement(463..1416)
                     /locus_tag="VFX38_08250"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX5314876.1"
                     /translation="MVRAESDDADTLAELLVSAARPLAPATLRTRLGWSRQRHAKAQR
                     VLQALGATLVASGNDVLLRLADPLDASAIRDSLPRSFAFDEIIVRRVCSSTNDCVRGQ
                     ASTVLCVAEVQTAGRGRRGKVWMQPFGSGLALSLGAPAPRGRLDPLAIAVATAAAMAL
                     EDAGYRGIGLKWPNDLYACGRKLGGVLVEVEGGFEPRVVIGLGLNVHGAPALADRDTV
                     ALGELSGPQPCRNVLAAGLAAALAHALERFEAEGFAPFAREFATLDVLVGQTITLSEG
                     DTALRGVARGIDEMGALVLDTDSGCLHRTAGEVTLGAWAGA"
     gene            complement(1403..2029)
                     /gene="plsY"
                     /locus_tag="VFX38_08255"
     CDS             complement(1403..2029)
                     /gene="plsY"
                     /locus_tag="VFX38_08255"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX5314877.1"
                     /translation="MIIFWIVLAIVLAYLVGALPGGIVLGRLAGVNLREFGSGNAGAT
                     NALRARGPAFGVAVFAFDCVKGVIAALLLPRLAAGIFWLPEACAAAVVVGHVFPVWFG
                     FRGGKGFATALGAIVVLSPLALAPVAGVWILVLLLSGYVGLATLLSAVAYPIFIAARL
                     ALHRPALLAFAIFLALFLIYTHRDNIRRLAAGEEHRFERVRLLGRGSR"
     gene            2159..2716
                     /locus_tag="VFX38_08260"
     CDS             2159..2716
                     /locus_tag="VFX38_08260"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5314878.1"
                     /translation="MEPAPQLPSGDTEETLRAVRRIARALALSSRDLARQHRLTPAQL
                     LCLRLLKDQPALNAGAIAAALSLSPQTMTGLLDRLEARGLATRLRSTSDRRQVLVRLS
                     ARGSEALDAAGPLLQDRFVARLEALPIPRRRSLRRALQAIVALLEADRLDAAPLLAPG
                     SRLEPSARHGSATTAAGAEATADRQ"
     gene            2760..3962
                     /locus_tag="VFX38_08265"
     CDS             2760..3962
                     /locus_tag="VFX38_08265"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX5314879.1"
                     /translation="MSLIVQKYGGSSVADPDHIRRVARRIRRARDAGHEVVAVVSAMG
                     DTTSELLDRAQTLSQNPTPRELDMLLSAGERIAMSLLAMALGEAGVDAISLTGPQAGI
                     HTDETHFNAKIARVEPKRVRAELERGRTVIVAGYQGLTPRGEVATLGRGGSDTTAVAL
                     AAALGAERCEICSDVDGIYTADPRVVTSAKRIERMAYEDMLALARHGARVLNARAVGY
                     ALRTATPVFSVSSFRDGGGTLVHHRRRRPGRAIGIASHPGLLRIEWNAAAERETLARA
                     LEVLDVREPFHYRASPARATHELMIPVEQLAEPAACGEQMRRAFDTNVKIGNTLGSVS
                     VVGPGLDRDESARQRFGAQVTRFQPAPVRRLAAPDALACILPEAALADATRALHREFI
                     ENAGREAA"
     gene            3959..4459
                     /gene="ectA"
                     /locus_tag="VFX38_08270"
     CDS             3959..4459
                     /gene="ectA"
                     /locus_tag="VFX38_08270"
                     /EC_number="2.3.1.178"
                     /inference="COORDINATES: protein motif:HMM:TIGR02406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="HEX5314880.1"
                     /translation="MRAVDDLRVRAPLAVDGKRIWSLVRSEGVLEDNSAYGYLLLCTH
                     FAAHGLVAERSGELAGFVTAYRPPDDPEALFVWQIGVAANARGCGLGRTMLVDLIRRP
                     ANRDARYLSATVAPGNGPSNRLFAAFARVENVRLETREGYAAELFPSAHEPEPMLRIG
                     PLAARA"
     gene            4486..>5029
                     /locus_tag="VFX38_08275"
     CDS             4486..>5029
                     /locus_tag="VFX38_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5314881.1"
                     /translation="MNIEPFTRLESEVRSYCRSFPVVFTRARGARLHAENGRRYLDFF
                     AGAGALNYGHNHPRLQEALIAYLREDGIAHSLDMYTAAKRRFLERFEEIILAPRALSY
                     RVQFPGPTGTNAVESAVKLARKITGRGNVIAFTNAFHGMTLGALALSGNRFKRGGAGV
                     PLGNATPMPYAGYLGDDGDTL"
BASE COUNT          886 a         1804 c         1594 g          745 t
ORIGIN      
        1 tcgacgcgag gcgcgtgccg ctcgcgagcg ctttgcgcat cagtccgatt ccgggcgcga
       61 tcaatccgcc gacgtgccgg ccctccccgg cgacgtaatc caccgtcacg gcggtaccgc
      121 aatcggcgag cagcacggcc ccgccatgcg cgcgatatgc ggcgatcatc gcggcccagc
      181 ggtcgatacc aagacgcgcg ggttccgcat aggcattgcg tacgccgcag gcctcgcggg
      241 tcgcaacgag ttcgcaggga tcaaggtccc agttcgcgcg gcatgcggcg agaaattctt
      301 ccgttaccgg ctgggccgcg acgctgccga caatcacttc cggcggcggt tcgctgccgg
      361 cgagcacgcc ggcgatgacc tgccccggtc tgccgtcatg tacgcgcaca tcggaaccgc
      421 ccgcaccgcg ccaggcaatg cgggtgctgc cgagattgac gatcaggcgc ctgcccatgc
      481 gcccagcgta acctcgccgg ccgtgcggtg caggcagccg ctatccgtat cgagaaccaa
      541 ggcacccatt tcatcaatac cgcgggcgac gccgcgcaac gccgtatctc cttcggagag
      601 cgtgatcgtc tgccccacga gcacatcgag agtggcgaat tcgcgcgcga acggcgcaaa
      661 tccttcggcc tcgaaacgtt cgagcgcatg cgcgagcgcc gccgcgagcc cggcggcgag
      721 aacgttgcgg catggttgcg gcccactcag ctcgccgagc gcaacggtgt cgcggtccgc
      781 aagcgccggc gcgccatgca cgttgagccc gagtccgatc acgacacgcg gctcgaagcc
      841 gccttcgacc tcgaccagca ccccgccgag tttgcgcccg caggcataca gatcgttggg
      901 ccatttgaga ccaatgccgc gataacccgc atcctccagc gccatcgctg ccgccgtcgc
      961 cacggcgatc gcgagcgggt cgagccgccc gcgcggggcc ggcgcgccga gcgacagcgc
     1021 gagtccactg ccgaaaggct gcatccagac cttgccgcgc ctgccccgcc ccgccgtctg
     1081 cacttcggcg acacacaaga ccgtggaagc ctgcccgcgc acgcaatcgt tggtggaaga
     1141 gcaaacgcgg cgaacgatta tttcgtcaaa agcgaaactc cgaggcaagg aatcccggat
     1201 cgcgctcgca tccagcggat cggcgagacg caagagcaca tcgtttccgc ttgcgaccag
     1261 cgttgcgccg agcgcctgca acactcgctg cgccttcgca tgtcgttgcc gactccagcc
     1321 gagccgcgta cgcagcgtcg ccggcgccag cggtcgcgcc gcggaaacga gcagttccgc
     1381 gagcgtgtcg gcgtcatccg attcagcgcg aaccacgacc aagcagccgt acccgctcga
     1441 aacgatgttc ttccccggcg gcgagccggc ggatattgtc acgatgggtg taaatcagaa
     1501 acagcgcgag aaaaatggcg aaggccagca aggcagggcg atgcaacgcg aggcgagccg
     1561 cgatgaaaat cggatatgcg acggcactca gcaaggtcgc caggccgacg tagccgctga
     1621 gaagcagaac cagaatccac acgccggcga ccggcgcgag cgcgagcggc gaaagcacga
     1681 cgattgcgcc gagcgcggtc gcaaacccct tgccgccgcg aaagccgaac cagaccggga
     1741 acacatgtcc gacgacgacc gccgccgcac aggcctcggg aagccagaaa atgccggccg
     1801 ccagacgcgg cagcaacaag gcggcgatca cgcccttcac gcaatcgaaa gcgaacacgg
     1861 cgacgccgaa ggccggcccg cgagcgcgca gcgcattggt cgcgcccgcg tttccgctgc
     1921 cgaattcgcg cagattcacc cccgcgaggc ggccgagcac gatcccgccg ggcagggcgc
     1981 ccacgagata ggcgagcacg atggcaagga cgatccagaa gatgatcatg cttctcctcg
     2041 gccgccattg tacggagtcc gtccgggacg gcggccgtcg gtttgcaatc aaatgctacg
     2101 cgtgttaaca tttcttgtca tgaactatcc acaagctgaa gttttggcgg acaaggccat
     2161 ggagccggcg ccgcaactgc catccggcga taccgaggag accctgcggg cggtgcgtcg
     2221 cattgcccgc gcgctggcgc tttcgagccg ggatctggcc cgccagcacc gcctcactcc
     2281 ggcgcaactg ctgtgtctgc gtctgctgaa ggatcaaccg gcgctcaacg ccggcgcgat
     2341 cgcggccgcc ctgtcgcttt cgccccagac gatgacgggc ctgctcgatc gcctggaagc
     2401 gcgcggactc gcgacccggc tgcgctcgac cagcgaccgc cgccaagtgc tggtgcgcct
     2461 gagtgcgcgc ggatcggagg ctctcgatgc ggccggaccg ctgctgcagg atcgtttcgt
     2521 cgcccgcctg gaagctctgc ccatcccgcg gcggcgcagc ttgcgacgcg cgctgcaagc
     2581 gatcgtcgcc ctgctcgaag ccgaccggct cgacgccgcg ccgctcctcg cgcccggctc
     2641 ccggctcgaa ccgagcgccc gtcacggcag cgccacaacc gccgcgggtg cggaagccac
     2701 tgcggaccgg cagtgacgct gctcgctctc ccttcgaatt catccggaat cttgtaaaca
     2761 tgagcctgat cgttcagaaa tatggcggct cctcggtcgc cgatcccgat cacatccgtc
     2821 gcgttgcgcg gcggatccgg cgtgcccgcg atgccggcca cgaggtggta gcggtcgtct
     2881 cggcgatggg cgacactacc tccgaactgc tggatcgcgc ccaaactctg tcgcagaacc
     2941 cgacgccgcg cgaactggac atgctgcttt ccgccggcga gcgcatcgcc atgtcgctcc
     3001 tcgcgatggc gctcggagaa gcgggtgtcg atgcgatttc actcaccgga ccgcaggccg
     3061 gcattcacac cgacgagacc catttcaatg cgaaaattgc gagagtcgag ccgaagcgcg
     3121 tgcgcgccga actggagcgg ggacgaaccg tcatcgtcgc cggctatcag ggcctgaccc
     3181 cgcggggcga agtcgccacg ctcggacgcg gcggctcgga cacgaccgcc gtagcactcg
     3241 ccgcggcact cggggcggag cgctgcgaaa tctgttcgga cgtggacggc atttacaccg
     3301 ccgatccgcg ggtggtcaca tccgcgaagc gcatcgagcg gatggcctat gaggacatgc
     3361 tcgcgctcgc ccgccacggc gcgcgcgtgc tcaatgcccg cgccgtcggc tatgcgttgc
     3421 ggaccgccac gccggtgttt tcggtttcga gcttccggga cggtggcggc acgctcgtgc
     3481 atcatcgccg gcgccgcccg ggccgcgcga tcggaatcgc ctcgcatccg gggctgctcc
     3541 gcatcgaatg gaatgccgcg gcggaacgtg agacgcttgc gcgggcgctc gaagtgctgg
     3601 acgttcgcga gcccttccac tatcgcgcgt cgcccgcaag ggcaacacat gaactgatga
     3661 tccccgtcga acagcttgcc gaacccgcgg cctgcggcga gcagatgcgc cgcgccttcg
     3721 acaccaacgt caagatcgga aacactctgg gttccgtgtc ggtcgtcggc cccgggctcg
     3781 atcgcgacga gtctgcgcgg caacgtttcg gtgcgcaggt cacgcgattc caacccgccc
     3841 cggtgcgccg cctcgccgct cccgacgcgc tcgcctgcat ccttcccgag gcagcactcg
     3901 ccgacgcgac gcgcgcgctg catcgcgaat tcatcgaaaa cgccgggcgc gaagcggcat
     3961 gagagccgtg gacgaccttc gcgttcgtgc accgcttgcc gtcgacggca agcgtatctg
     4021 gtcgctcgtg cgcagcgagg gcgtgcttga ggacaactcc gcctacggct atctgctgct
     4081 atgcacgcat ttcgccgcgc atggactcgt cgccgaacgc agcggcgaac ttgcgggctt
     4141 cgtcaccgcc taccggccgc cggacgatcc cgaagcgctc ttcgtgtggc agatcggggt
     4201 cgccgccaat gcgcgcggct gcggactggg acgcacgatg ctcgtggatt tgatccgacg
     4261 tccggccaac cgcgacgccc gatacctgag cgcgacggtg gcgcccggca acggcccctc
     4321 gaatcggctc ttcgccgcct tcgcccgcgt cgagaacgtc cggctggaaa cccgcgaagg
     4381 ctatgccgcg gagctgttcc catccgctca cgagccggag ccaatgctgc gcatcggtcc
     4441 gcttgccgcg cgcgcctgaa ccttccggag gaaatggaaa tgagcatgaa tatcgaaccc
     4501 tttacgagac tcgaatcgga ggtgcgcagc tactgccgca gcttcccggt cgtcttcacc
     4561 cgcgcccggg gcgcccgtct ccacgccgaa aacgggagaa ggtatctcga ctttttcgcc
     4621 ggcgccggtg cactcaatta tggccacaac cacccgcgac ttcaggaagc gctcatcgca
     4681 tatctgcgcg aggacggcat cgcccacagc ctcgacatgt acacggcggc caaacgccgc
     4741 ttcctcgaac gcttcgagga aatcatcctc gccccgcgcg cactctccta ccgcgtgcag
     4801 tttccgggac cgaccggaac caatgcggtg gaatccgccg taaaactcgc gcgcaaaatc
     4861 accggccgtg gaaacgtcat cgccttcacc aatgcctttc acggcatgac gctcggcgcg
     4921 ctcgcgctct ccggcaaccg cttcaagcgc ggcggcgccg gcgtgccgct cggcaacgcg
     4981 acgcccatgc cttacgccgg ctatctcggc gacgacggcg acaccctcg
//
LOCUS       DASUJG010000389         2680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_113098, whole genome shotgun sequence.
ACCESSION   DASUJG010000389 DASUJG010000000
VERSION     DASUJG010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113098"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VFX38_08280"
     CDS             <1..604
                     /locus_tag="VFX38_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314882.1"
                     /translation="LAPEESASLIGAAREHFADADFLLEAGSSGRWYFSAAGIADARL
                     APPDEAQTGTFEAIPAGADGRRLARMLTELQMLWHGHPVNRARHRDGRPEANALWVWE
                     GGRLPPSPRLAAQPTLFASAPEISGLALWAGLQHSPLDSFDAVSSDGVPVVAIEADEG
                     ALGSEWLRRFDARRREFRLFVDGAVQALPARRGLFASRSR"
     gene            666..1764
                     /gene="prfB"
                     /locus_tag="VFX38_08285"
     CDS             join(666..740,742..1764)
                     /gene="prfB"
                     /locus_tag="VFX38_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_319006094.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX5314883.1"
                     /translation="MAEFAPTREKIADLESRNEALRRYLDYDGSRERLAEVERSLEAP
                     EVWSDPAQAQHLGRERVRLAGIVTTLDEVANSLRDSSDLLELAAEEDDAQALAQIDAD
                     VAASAARTGELEFRRMFAGEMDAANAFLDIQSGSGGTEAQDWSEMLLRMYLRWGERRG
                     FVTELIEVSPGEVAGIKSATIRFAGNYAYGWLRTETGVHRLVRKSPFDSGHRRHTSFA
                     AVFVSPEVDDDIEIEVNPADLRVDVYRASGAGGQHVNRTESAVRITHLPTGIVVQCQN
                     ERSQHKNRATAMKQLKARLYELELSKRRSAQQELEEAKSDIGWGSQIRSYVLDQSRIK
                     DLRTGIEIANTQGVLDGALDPFIEAALKAGV"
     gene            1928..>2680
                     /locus_tag="VFX38_08290"
     CDS             1928..>2680
                     /locus_tag="VFX38_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX5314884.1"
                     /translation="MTDRNERHDLVAERRQKLAALRAEGFAYPNDFRPDTTIASLLGA
                     FGELSEAALAGRPLWVRIGGRMMTRRIMGGASFAHLDDGSGRLQIFAQGESLGAEGYE
                     RFKSLDLGDLVGIEGQLFRTRTGELTLRASGLRLLVKCLRPLPEKFHGLKDPELRYRQ
                     RYLDLAMNPDVREIFRIRAEITRVTRALLDAWGFLEVETPMMQPLAGGALARPFVTHH
                     NALDIPLYLRIAPELYLKRLLVGGLARVYEINR"
BASE COUNT          437 a          889 c          879 g          475 t
ORIGIN      
        1 gctcgcgccc gaggagagcg cgtcgttgat tggcgccgct cgcgaacatt tcgccgacgc
       61 cgattttctc ctcgaagcgg gctcctcggg gcgctggtat ttttccgccg cgggcatcgc
      121 cgacgcgcgg cttgccccgc cggatgaagc gcagaccggg accttcgagg ccattcccgc
      181 cggagcggat gggcgccggc tcgcgcgcat gcttaccgaa ctgcagatgc tctggcatgg
      241 gcatccggtc aaccgggcgc gccatcgcga cggacgtccg gaggcgaatg cgctctgggt
      301 ctgggagggc gggcggcttc cgccgtcgcc acggctcgcg gcgcagccga ctttattcgc
      361 cagcgcgccg gagatctccg gtcttgcgct ctgggcggga ctacagcact cgccgctcga
      421 ctcgttcgac gcggtttcgt ccgacggtgt gccggtggtg gcgatcgaag cggacgaagg
      481 cgccctcggc agcgaatggc ttcgccgttt cgatgcgcgg cgccgcgaat tccgcctttt
      541 tgtcgacggc gccgtgcagg cgttgccggc acgccgcgga ctcttcgctt cgcgatcgcg
      601 ctgattgcgg ccaggcgatc tccttgcttc gttacaatac gttcccgcaa cgttgcgaga
      661 ctaccgtggc cgagtttgcc ccgacccgcg aaaaaattgc cgatctcgag agccgcaacg
      721 aggctctgag gaggtatctt tgactacgat ggaagccgcg aacggctcgc cgaggtagag
      781 cgctcgctcg aagcgccgga ggtctggtcc gatccggcgc aggcgcaaca tctcgggcgc
      841 gaacgggtgc gcctcgccgg catcgtcacg acgctcgacg aggtggcgaa ctcgcttcgc
      901 gactcctcgg acctgctcga actcgcggcc gaggaggacg acgcgcaggc gctggcgcag
      961 atcgacgccg acgtcgcggc gagcgcggcc cgcaccgggg aactcgaatt ccgccgcatg
     1021 ttcgccggcg agatggacgc ggcgaatgcc tttctcgata ttcagtcggg ctccggtggg
     1081 acggaggccc aggactggtc ggagatgctg ctgaggatgt atctgcgctg gggcgagcgg
     1141 cgcggtttcg tgactgaact catcgaagtg tcacccggcg aagtcgcggg gatcaagagt
     1201 gcgaccatcc gcttcgccgg caactatgcc tacggctggc tgcgcaccga aaccggcgtg
     1261 catcgcctcg tgcggaaatc cccgttcgac tcgggccatc gtcgccacac ttccttcgcg
     1321 gcggttttcg tgtcgccgga agtggacgac gacatcgaaa tcgaggtcaa tccggccgat
     1381 ctgcgcgtgg acgtgtatcg cgcgagcggc gccggcggcc agcacgtgaa ccgcaccgaa
     1441 tcggccgtgc gcatcacgca tcttccgacg ggcatcgtcg ttcagtgtca gaatgagcgc
     1501 tcgcagcaca agaaccgggc gaccgcgatg aagcagttga aggccaggct ttacgaactc
     1561 gaactctcca agcggcgcag cgcccagcag gaactcgagg aggccaagag cgacatcggc
     1621 tggggcagcc agattcgttc ctatgtgctc gatcagtcgc ggatcaagga tctgcgcacc
     1681 gggatcgaga tcgcgaacac ccagggcgtg ctcgacggcg cgctcgaccc cttcatcgaa
     1741 gcggcgctca aggcgggagt ctgaaatgtc tcgatgcagc gtacgagaaa gcctgctcgc
     1801 gatttcttcc gacctccccc ttcgcccgcg atatttcctg cctcccgcat cgcgagcgac
     1861 gccatcctgc tcccctttgc ttgcgaaggg ggaccgaggc ggatgtcctc gatcatgagg
     1921 tgtcgacatg accgacagaa atgaacgcca cgatctcgtc gccgaacggc gccagaagct
     1981 cgccgcgctg cgcgcggagg gcttcgccta tccgaacgac ttccgtcccg acaccacgat
     2041 cgcatcgctg ctcggagcct tcggcgagct gagcgaggcc gcgctcgcgg ggcggccgct
     2101 ctgggttcgc atcggtggac gcatgatgac gcggcggatc atgggcggcg cgagcttcgc
     2161 gcacttggac gatggcagcg gccgcctgca gattttcgcg caaggcgaat cgctcggtgc
     2221 ggaaggctat gagcgcttca agtcgctcga tctcggggat ctcgtcggca tcgaaggtca
     2281 actctttcgt acgcgcaccg gcgaactcac gctccgcgcc tcgggcctgc gtctgctcgt
     2341 gaagtgtctg cgcccgctgc ccgagaagtt ccatggtctc aaggatccgg agctgcgcta
     2401 tcgccagcgt tatctcgatc tggccatgaa tcccgacgtg cgcgagatct tccgcatccg
     2461 cgcggaaatc acgcgcgtca cccgcgcgct tctcgacgcc tggggctttc tcgaagtcga
     2521 aacgccgatg atgcagccgc tcgcgggcgg cgcgctggcg cgtcccttcg tcacccatca
     2581 caatgcgctc gacattccgc tttatctgcg catcgcgccc gaactgtatc tcaagcgcct
     2641 gctcgtcggc ggcctcgccc gggtgtacga aatcaaccgt
//
LOCUS       DASUJG010000390         2248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1092133, whole genome shotgun sequence.
ACCESSION   DASUJG010000390 DASUJG010000000
VERSION     DASUJG010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092133"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="VFX38_08295"
     CDS             complement(<1..1150)
                     /locus_tag="VFX38_08295"
                     /inference="COORDINATES: protein motif:HMM:NF025670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HEX5314885.1"
                     /translation="MTMHCKIALSAGFFLVLLPAAAMPIGTGRAVAAGSSHSDRLPEK
                     LRSAFYEALATDAGAAYAIDRRACVSLPKPALKACFDRSGASFTGGGTPLALRLAAWG
                     RGARLVAVAEVSPSVEGNRVSFVHGNLAEWWRVLPIGFEQGFTIAKRPPGGGELKLAL
                     ATSREAGATAGQNTVAWGKLRYGQLVVTDARGHALPATLKTQGKRIVVAVNDLHAVYP
                     LTVDPLVWLEQEVSANDGAAGDSFGWAVALSGRTALIGAPFATVDGNAYQGAAYVFTE
                     SNGRWAQTSKLVASDGESDDEFGIWVSLAGTTALVGAPFGDGAHGAAYVFHRSGGVWA
                     QTGRLSANDGASGDHFGWSVATDGASALVGAPFATVANHGDQGAAYVFA"
     gene            complement(1341..1874)
                     /locus_tag="VFX38_08300"
     CDS             complement(1341..1874)
                     /locus_tag="VFX38_08300"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014402262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HEX5314886.1"
                     /translation="MQAPASGDAAGPCARVAVFTTGGTLDKVYFDALSRYEVGTPVVT
                     GILSEAGVTAPYTVTMLMQKDSLDLSDADRGAIRSAIEQADAKWILVTHGTDTMIETA
                     RVLTDIPGKTIVLTGALKPARFRDSDAVFNVAAAFVAVQILPPGAWIVMNGRVFDPAR
                     ARKNRDANRFEESESGE"
     gene            1935..>2248
                     /locus_tag="VFX38_08305"
     CDS             1935..>2248
                     /locus_tag="VFX38_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004371159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEX5314887.1"
                     /translation="MRLIVASVFAAMLLASGAGPEANAAPAPSPRLLAIDGAIGPPMA
                     DYIGRTLAEAAKAKAPFVVIRLDTPGGLSDSMRSIIQDILASPVPVVCWVGPQGARAA
                     SAG"
BASE COUNT          382 a          775 c          718 g          373 t
ORIGIN      
        1 ccgcgaatac ataggctgca ccctgatcgc cgtggttggc gaccgtggcg aagggagcgc
       61 cgacgagagc gcttgcgccg tcggtcgcga ccgaccatcc gaaatggtcg cccgaggcgc
      121 cgtcattcgc gctgagccgc cccgtctgcg cccagacgcc gcctgaacgg tggaagacgt
      181 aggcagcacc atgagcaccg tctccaaacg gtgcgccgac cagcgcagtc gtgcccgcga
      241 gcgaaaccca gatcccgaac tcgtcgtccg attcgccgtc gctcgcgacg agcttggacg
      301 tctgtgccca tctcccgttc gactcggtaa atacgtaggc cgcgccttga taggcattgc
      361 catcgaccgt ggcaaagggg gcgccgatga gcgcggtgcg gccggaaagg gcaaccgccc
      421 agccgaaact gtcgcccgcc gccccgtcgt tcgcggaaac ctcctgctcc agccacacca
      481 gcggatcgac cgtcagcgga tagacggcat gaaggtcatt tacggcgaca acgatgcgct
      541 tgccctgcgt cttgagggtc gccggcaacg catggccccg tgcatcggtg acgacgagtt
      601 ggccgtaacg caacttcccc caggccacgg tattttgccc cgccgttgcg ccagcctccc
      661 ggctcgtcgc aagcgcaagc ttcagctcgc ctccgcccgg cgggcgcttc gcaatcgtaa
      721 agccctgctc gaatccaatc ggcaacaccc gccaccactc ggcgagattg ccatgcacaa
      781 aagacacccg atttccctcg accgaagggc tcacctcggc aacggccacg agtctcgccc
      841 cgcggcccca ggctgcgagc cgcagcgcga gcggcgttcc gccgcccgtg aagcttgcgc
      901 cggatcggtc gaaacaagcc ttcagtgcgg gcttcggcag gctcacgcaa gctctgcggt
      961 cgattgcata agcggctccg gcgtccgttg cgagcgcttc gtagaatgcc gaacgcaact
     1021 tctccggcag cctgtccgaa tgggacgatc ccgctgcaac agcacgaccc gtgccgatcg
     1081 gcatggcggc cgcgggcaga agcacaagga aaaacccggc gctcagggca attttgcaat
     1141 gcatggtcat gaaaatctcc cggtaaccgg atggattgcg cgcccggcga caatgcgctc
     1201 cgcgcacagt ttcgactata cacccgcaac cgttgccggc aaagcgcttg ctcacatcca
     1261 ccgcttggga taaatgtcgc cgcgatccgc agacgcgggt gggtgttcgc tcgcggagca
     1321 agcccgcggc tgcgacgctg ctattccccg ctttccgact cttcgaagcg gttcgcgtcg
     1381 cgattcttgc gcgcgcgagc cggatcgaaa acgcggccgt tcatgacgat ccaggcgccg
     1441 ggcgggagaa tctgcaccgc gacgaaggcc gcggcgacat tgaatacggc gtcgctgtcg
     1501 cgaaagcgcg ccggcttgag cgcgccggtg agcacgatcg tttttccggg aatatccgtc
     1561 agtacgcgcg cggtttcgat catggtgtcc gtgccgtgcg tcacgaggat ccatttcgcg
     1621 tcggcctgct cgatggcgct gcggatcgcg ccgcgatcgg cgtcgctgag atcgaggctg
     1681 tccttctgca tcagcatcgt cacggtgtag ggtgcggtca cgcccgcctc gctcaggatt
     1741 cccgtgacga ccggtgttcc cacttcgtag cggctgaggg catcgaaata caccttgtcg
     1801 agggttccgc cggtggtgaa caccgcgacc cgggcgcaag ggccggcggc atcgcccgac
     1861 gcgggcgctt gcatcgcgca cgcgcttctg ttgtgatgca cggctagcgg ctccttccga
     1921 accggaaaac ggcaatgcgg ctcattgtag cgagtgtttt tgcggcgatg ctgctggcgt
     1981 ccggcgcagg gccggaggcg aacgccgcgc cggcgccctc gccgcgtctg ctcgcgatcg
     2041 acggcgccat cggcccgccc atggccgatt acatcggacg cacgcttgcc gaagcggcaa
     2101 aagccaaggc ccccttcgtg gtcatccgcc tcgacacccc gggcggactt tcggattcga
     2161 tgcgcagcat catccaggac attctcgcct cgccggtgcc ggtggtgtgc tgggtcgggc
     2221 cgcagggcgc gcgggccgcg agcgccgg
//
LOCUS       DASUJG010000391         2604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_481360, whole genome shotgun sequence.
ACCESSION   DASUJG010000391 DASUJG010000000
VERSION     DASUJG010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481360"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="VFX38_08310"
     CDS             complement(<1..143)
                     /locus_tag="VFX38_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018138759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5314888.1"
                     /translation="MGFLAGKRALIVGLASQRSIAWGIAGAMHREGARLAFGYQGEKL
                     AERA"
     gene            complement(158..2164)
                     /locus_tag="VFX38_08315"
     CDS             complement(158..2164)
                     /locus_tag="VFX38_08315"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX5314889.1"
                     /translation="MTMRTTRTLSAAFAIAALAALAGCAGMQSPVAGGDTNSTTAASA
                     PGSVPIPIATLLDDAQSQADDDAATARLAALAGAIGGPDANLWGVMRQKFSLPDGNET
                     QVREQLSFFRGRSSFIQHSAQRAEPYLYYIVSQLEAHNMPVDLALLPIVESGFNPYAY
                     SPGHAAGLWQFISSTGRIYGLKQDSWYDGRLDVVAATGAALKYLENLHDQFDGNWLLA
                     LAAYNSGSITVQQAIDYNRRHGLPTDFWHLNLPAQTRAYVPRLLAIRDIIANPGKFGV
                     TLPFIANVPCLAEVDLKGQLDLAIAAKFAGMDLKDLYRINPGYSRWATPPDGPFTLLL
                     PIDKKSQFLAALDAKTQLERAPGWISHRVRSGETLSGIAANYHTSVASLEEHNQLRGS
                     VIRVGAALKVPTSGSGNNLRYVTDQPMQVAYDYSPPTPGRSSRSSGDEPSYSHIRVRS
                     GDSLWGVAKTYHVSVADLARWNHLSTSARLRTGQTLAVLATASGASAPAGGHYVVRSG
                     DSLSTIAARYHVSISDLANWNSIGSGSVIHPGQQLLIGGDGDKEAAVASTSYVVKSGD
                     SLSAIASRFDVSTGDLAAWNSLSRTSVLHPGQTLEIHGAAAADVAANQARRIHYVVRS
                     GDSLYAISNRFNVSVAKLTDWNSLSAASILHPGQLLTLFVSASN"
     gene            complement(2174..>2604)
                     /locus_tag="VFX38_08320"
     CDS             complement(2174..>2604)
                     /locus_tag="VFX38_08320"
                     /inference="COORDINATES: protein motif:HMM:NF027449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase C-terminal
                     domain-containing protein"
                     /protein_id="HEX5314890.1"
                     /translation="AGHVAYYGPGLLFCGDTLFSAGCGRLLEGTPAQMYRSLRRLAAL
                     PTRTQVYAAHEYTVENLRFACSLLPADSRLARALENAVTIRADAQATLPTTIGREREI
                     NLFLRVDEPELRAAIAERTGGSLPAHQEAFAALRRLKDSF"
BASE COUNT          448 a          806 c          897 g          453 t
ORIGIN      
        1 gcccgctcgg cgagcttttc gccctgatag ccgaaggcga gccgggcgcc ctcgcgatgc
       61 atggcgccgg cgatgcccca ggcgatggaa cgctgactcg caagtccgac gatcagcgcc
      121 cgtttgccgg caagaaatcc cacccgcagg ctcgcggcta gttgctggcg ctgacgaaca
      181 gggtcaacaa ctggccggga tggaggatgc tcgcggccga aagcgagttc caatccgtga
      241 gtttggcgac cgatacattg aagcgattcg aaatcgcgta gagcgaatct ccgctccgga
      301 ccacgtagtg aatgcgccgc gcctgattcg cggccacgtc ggccgctgcg gccccgtgaa
      361 tttcgagcgt ttggccggga tgaagaacgc tcgtccgcga gagggaattc caggcggcga
      421 gatctccggt cgagacatcg aagcggctgg cgattgccga gagcgagtcg ccgcttttca
      481 cgacatagct tgtcgacgct actgctgctt ccttgtcgcc gtccccgccg atcagcagtt
      541 gctggccggg atggatgacg ctcccgctcc cgatcgaatt ccagttcgcc agatcgctga
      601 ttgacacgtg atagcgagcc gcgatcgtcg aaagcgagtc gccgctgcgc accacgtaat
      661 ggccgccggc cggcgcgctt gcgcccgaag ccgtggcgag cacggcgagg gtttgccccg
      721 tgcgcaggcg ggcgctggtg ctcaggtgat tccagcgcgc gagatcggcc acgctcacgt
      781 gataggtctt tgcgaccccc caaagcgagt cgccactgcg cacccgtata tgagaatacg
      841 acggctcgtc cccgctgctc cggctgcttc gtccgggcgt gggggggctg tagtcatagg
      901 cgacctgcat cggctgatcg gtcacgtagc gaaggttgtt gccgctgccc gaggtcggaa
      961 ccttgagggc tgcgcccacg cgaatcacgc tgccgcggag ctggttgtgc tcctcgaggc
     1021 tcgcgacgct ggtgtggtag ttcgccgcga tgccgctcag ggtctcgccg ctgcgtaccc
     1081 gatggctgat ccagcccggc gcgcgctcga gttgcgtctt ggcgtcgaga gcggcgagga
     1141 actgcgattt cttgtcgatc ggcagaagca gcgtgaaggg accgtccggc ggggtcgccc
     1201 agcgactgta gccgggattg atccgataga gatccttgag gtccatgccc gcgaacttgg
     1261 ccgcgatcgc cagatcgagc tggcccttga ggtccacttc cgcgagacag ggaacattgg
     1321 cgatgaaggg cagggtcacg ccgaatttgc cggggttggc gatgatgtcg cgaatcgcga
     1381 gcagccgcgg cacgtaggct cgcgtctgcg ccggaaggtt gagatgccag aagtcggtgg
     1441 gcagcccgtg ccggcggttg taatcgatcg cctgctgcac ggtaatggac ccggagttgt
     1501 aggcggcgag cgcgagcagc cagttgccgt cgaattggtc gtgcaggttc tcaagatact
     1561 tcagagcggc gccggtcgcc gctaccacgt cgaggcggcc gtcgtaccag ctgtcctgtt
     1621 tcagcccgta gatgcgcccc gtcgaggaga tgaattgcca cagccccgcg gcgtgtcccg
     1681 gcgaataagc gtagggattg aagccgcttt cgacgatggg aagaagcgcg aggtcgaccg
     1741 gcatgttgtg tgcttcgagc tgcgagacga tgtagtagag atacggttcg gcgcgctgcg
     1801 cagaatgctg gatgaagctg ctgcggccgc ggaagaacga cagttgctcg cgcacctgcg
     1861 tctcgttgcc gtcgggcagc gaaaacttct ggcgcatgac accccagagg ttcgcatcgg
     1921 gcccgccaat ggcacccgcg agcgccgcga ggcgcgccgt ggcggcgtca tcgtcggcct
     1981 gggactgggc gtcgtcgaga agcgtggcga tcgggatcgg gaccgaaccc ggtgcgctcg
     2041 cggcggtggt ggagttggta tcgccgcccg cgaccggaga ttgcatgccg gcgcaacccg
     2101 cgagcgcggc gagagcggca atggcgaagg cggccgagag agtcctggtc gtgcgcatcg
     2161 tcatgtcctc tggctagaac gagtctttca atcggcgtaa tgccgcaaat gcctcctgat
     2221 gcgccggcag ggaaccgcca gtcctttccg cgatcgccgc gcgcaactcc ggctcgtcga
     2281 cccgcaaaaa aagattgatc tcacgctccc gcccgatcgt cgtgggcagc gttgcctgcg
     2341 catccgcgcg gattgtaaca gcattttcca gcgctcgtgc aagcctgctg tcggcaggga
     2401 gaagcgagca cgcgaaccgc aggttctcga cggtatattc gtgggccgca tagacttgcg
     2461 ttcgcgtggg caacgccgcg agccggcgca gcgagcggta catctgcgcg ggcgtccctt
     2521 ccaacagtcg cccgcagccc gccgagaaca gcgtatcgcc acagaagagc agaccggggc
     2581 cgtagtacgc tacatgcccg gccg
//
LOCUS       DASUJG010000392        10603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_601443, whole genome shotgun sequence.
ACCESSION   DASUJG010000392 DASUJG010000000
VERSION     DASUJG010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 10603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10603
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601443"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VFX38_08325"
     CDS             <1..415
                     /locus_tag="VFX38_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEX5314891.1"
                     /translation="LLGDVAPSSVRSYLRLNTPEIFKRNGRGLYTLREQGIQESRPVP
                     YSPPSRRAPLPAYHFGRSSLYLCDCFPWLLQRGANSIHAVVTDPPYGLFEYSAEQQRK
                     LRAGRGGVWRIPPSFDGTIRSPLPRFTVLTDSDLR"
     gene            452..1054
                     /locus_tag="VFX38_08330"
     CDS             452..1054
                     /locus_tag="VFX38_08330"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEX5314892.1"
                     /translation="MAVLVPGANVIVASNPLLSHVVAGALSAVGLERRGEIARLVTTM
                     RGGDRPKDAHEEFSDVSVMPRSMWEPWVLFRKPLEGRVQDNLRKWKTGGFRRPTGNQP
                     FGDVIRSAPTHKSERGLAPHPSLKPQGFLRALVRGALPLGEGVVLDPFAGSGSTLAAA
                     EYAGYESIGIEKDAHYFRLAKKAIPKLSAFKNGNNYSLNL"
     gene            complement(1036..1644)
                     /locus_tag="VFX38_08335"
     CDS             complement(1036..1644)
                     /locus_tag="VFX38_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314893.1"
                     /translation="MTIHRNRVNAQQFNAVAQLPYELRLRDFEMAMQDVYDFFFDVNR
                     GLVERGLMRLDDTIRPAILSGLLSDMLTSSLAKHSRTLVVNSYHNGHPDLVVAGVYPR
                     NSVKAGTEGVDIKTTRKHDGAVDTHGARDQWMCVCVYDVDNQTEPARERRPMSFREVY
                     LGQVMGADFRRNPRGELGTRTATLHREGIENPHRNWVYRLSE"
     gene            complement(1641..3239)
                     /locus_tag="VFX38_08340"
     CDS             complement(1641..3239)
                     /locus_tag="VFX38_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012328952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEX5314894.1"
                     /translation="MQPWAIGVFVALFALVTLVGFFAARWRRGPLDRLEEWGLGGRRF
                     GTVVTWFLLGGDLYTAYTFVAVPALVYGKGAIGFFALPYTLLVFPLVYVVFTRLWSVA
                     RRHGYITAADFVHGRFGSRTLAALVAITGILATMPYIALQLVGIQVVVAALGFTTHGW
                     LADMPLVIAFLVLAAYTYTSGIRAPALIAIVKDALIYLTVIVAVVVIPLHLGGFGAIF
                     ARIPEKMLYLAGPSQGSLGAMSAYATLALGSALALFLYPHAVTGLLSARSRGTVKRNM
                     ALLPAYSLALALIAGLGFMAYASGVGDLPAYSGYFHQYGPNFAVPALFLHNFPGWFVG
                     IAFAAIAIGALVPSAIMSIAAANLFTRNLWPYLARRKPDEAGEARIAKIVSLLVKIGA
                     LAFIIFLPNQYAIQFQLLGGIWIIQTLPSVVLGLYGRWFHARALIAGWIAGMLVGTGM
                     AWTQHFTSVIFPLPLGPLSIPGYAALYSVIANLAIAVAGTLLLDRLGVARGPDRTAAV
                     DYSFETEEPGRTEVLPAEPIQEFP"
     gene            complement(3244..3450)
                     /locus_tag="VFX38_08345"
     CDS             complement(3244..3450)
                     /locus_tag="VFX38_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3311 domain-containing protein"
                     /protein_id="HEX5314895.1"
                     /translation="MNPERHAGPFAKTLIAILFLGCCAIALAVPLYNRARPALFGVPF
                     FYWFQFLWIIVTGAATALAYRLRI"
     gene            complement(3514..4209)
                     /gene="queE"
                     /locus_tag="VFX38_08350"
     CDS             complement(3514..4209)
                     /gene="queE"
                     /locus_tag="VFX38_08350"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018140611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEX5314896.1"
                     /translation="MNETSGPMRSLRRDELPPAERLRLTEIFLSIQGEAASVGWPTVF
                     VRLTGCPLRCRWCDTAYAFHGGEWRSFDAIEAEVARYAVSHVCITGGEPLAQANCRGL
                     VARLAAAGYAISIETSGALPIAGLDPRAIIVMDVKTPGSGEVSRNRLENLEQLRARDQ
                     AKFVLAERGDYEWAREFLREHDLAARCEVLFSPVAGELAPRALAEWILADRLPVRFQL
                     QLHKLLWGDVPGH"
     gene            complement(4334..5191)
                     /gene="ybgF"
                     /locus_tag="VFX38_08355"
     CDS             complement(4334..5191)
                     /gene="ybgF"
                     /locus_tag="VFX38_08355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEX5314897.1"
                     /translation="MLLALGALAAAGALGGCSSAVSPDDPVYQQIDQLQTKVDRLQHV
                     VGGQAMMNMASQQQQSSEQLSTLQGEVQDLEHRLQQMQSHQQLVNRDFDQRLAALGQG
                     STLATPGVGAGPGAGNGAGSAGGNYFPTGPIALAASASAPPVASANASATPTPSQGAD
                     SEAYQAAFDQLRAGNYSAAVRGFDAFIKKYPQSSYVPNAWYWMGETHYVNGEYKDAID
                     NFQQVVTKYPSSAKASDAYLKMGYAQYALKDYNGAQATFKSVISHYPGTPAASLAQQR
                     LQQMGAPGQ"
     gene            complement(5200..5745)
                     /locus_tag="VFX38_08360"
     CDS             complement(5200..5745)
                     /locus_tag="VFX38_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX5314898.1"
                     /translation="MNRNTNKVLVTFAFAGMLVLAGCASGPKKAPMAGSAPAAGSTAG
                     ALGAGMGNAQAIQAGPQSSYQNRTVYFDFNQSVVKPQYFPLLKAQAAYLVANSGASVV
                     LQGYCDDRGTWDYNIGLGSRRAVAVQRFLELQGVSDSQIKTISYGEAYPADSANNEAA
                     WAQNRRVVIVFGTTPAPTATP"
     gene            complement(5785..7128)
                     /gene="tolB"
                     /locus_tag="VFX38_08365"
     CDS             complement(5785..7128)
                     /gene="tolB"
                     /locus_tag="VFX38_08365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HEX5314899.1"
                     /translation="MKHSIALLFAGVLALVLTFVPARADLVIKITQGFSQPTPIAVVP
                     FAWQGLGPAPVDVAKVIGDDLERSGLFAPLPRASMISRPSAANDINFTDWKALSVNDL
                     VIGGVNVANDQARVRFQLFNVYTEQQLLGYLLPGPADNLRFTAHVAADMIYQRLTGIR
                     GAFATRIAYVRNEDAGKGRTRWELVIADADGANTQTVVTANDLIMSPAWSPDGTQIAY
                     VEYGANGSAIYLQDVKTGARKELIARPGINGAPAFSPDGQKLALVLSTHPADPDIYVM
                     DLASGRLAQITANPAIDTEPAWLPNGQGLIFTSDRGGNPQLYQIALDGSGAERITWQG
                     KYNARAAVSPDGKSIAFVHRDQGGALTIAVMDRGSGNLRELTPGPLDVSPSFAPNGAL
                     ILYGSQHGEQHVLATVSVNTGVREELSGSSGGLSQPAWGPFPPTPPGSPSATGVK"
     gene            complement(7144..8055)
                     /gene="tolA"
                     /locus_tag="VFX38_08370"
     CDS             complement(7144..8055)
                     /gene="tolA"
                     /locus_tag="VFX38_08370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HEX5314900.1"
                     /translation="MRSADSSRLRRNAVPFALALLLHVIVGVLVFVGVRGGAINPQAP
                     PGPGNEPQPIKAVVVNESDYQAAQQEIAQTQQARTEEVERLKKEAEDARQSREKTEQQ
                     LADLRQQKQSAATAAAEQQQQLQQVSEQAKQAAAAKEAQQKKLQELQAQTAAAKKAQE
                     EEAARLQKIQDQREAAEKAAAEAKAKAAAQAEAQRKAQLQAQIQAEENQRNAAALGLW
                     TAAIKAKVNSNWNRPPSTPPGLDCFVKITQLPDGEVVSAVVQQCNGDAAVRQSVQTAI
                     LKSSPLPIPDDPSVFQRTITFEFNPGP"
     gene            complement(8052..8504)
                     /locus_tag="VFX38_08375"
     CDS             complement(8052..8504)
                     /locus_tag="VFX38_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HEX5314901.1"
                     /translation="MSRRRRLMSQINVVPYIDVTLVLLIIFMITAPLLTRGVKVNLPQ
                     AAAQALSEKEMERHQPVVLSVDPGGRLYLNIGAHPDAPLSPDMIVAMVTVVLRQTPDT
                     PVLVRGDEAASYADVVRGMTLLQSAGAKNIGLITKTPSSPLVRKPGQP"
     gene            complement(8501..9190)
                     /gene="tolQ"
                     /locus_tag="VFX38_08380"
     CDS             complement(8501..9190)
                     /gene="tolQ"
                     /locus_tag="VFX38_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010560440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEX5314902.1"
                     /translation="MSTNFSIYSMIASASIVVQAVLVILVIASIFSWAVIFRKYAELK
                     RAEANAAKFEGEFWSGADLGELYKRLDSRASGGGLAEMFRSGFREFARLIRHGHPGAA
                     NALANARRAMAATRSRELDRLETSMGYLATVGSTAPYVGLFGTVWGIMAAFQGLGNAE
                     QATLAMVAPGISEALIATAMGLIAAIPATIFYNRFANQVDRLDGRYESFTEEFTNVLE
                     HHRVAEGDATS"
     gene            complement(9187..9606)
                     /gene="ybgC"
                     /locus_tag="VFX38_08385"
     CDS             complement(9187..9606)
                     /gene="ybgC"
                     /locus_tag="VFX38_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HEX5314903.1"
                     /translation="MNEAVKPFVWPVRVYYEDTDLGGVVQHAQYVKFLERARTEWLRA
                     GGIEQDTLMRETEILFVVTRVDIRYRRPAHFNDLLEISVALTSATKTRFVCEQKVARA
                     GAAAEPLVVASVEIACVDRPSFRARALPPILLARMAS"
     gene            complement(9599..10591)
                     /gene="ruvB"
                     /locus_tag="VFX38_08390"
     CDS             complement(9599..10591)
                     /gene="ruvB"
                     /locus_tag="VFX38_08390"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX5314904.1"
                     /translation="MREEPRLRPQRLAEYIGQDQVRERMEIFIAAARGRGETLDHCLL
                     FGPPGLGKTTLAHIIAHEMGATLHQTSGPVLERAGDLAAILTNLERGDVLFVDEIHRL
                     SPVVEEALYPALEDFVLDIMIGEGPAARSIRLDLAPFTLVGATTRAGLLTSPLRDRFG
                     IVSRLDFYDPAALEAIVRRSAGILGVRLEPAGAHEIAGRARGTPRIVNRLLRRVRDYA
                     EVRADGTVTEEVARAALDFLEVDRRGFDALDRRLLAIVIEHFDGGPVGVDSLATSLGE
                     ERGTIEDVIEPYLIQQGFLKRTPRGRVATARAWQHLGLAPPARDADLFAGAGDE"
BASE COUNT         1899 a         3374 c         3498 g         1832 t
ORIGIN      
        1 attgctgggc gacgtcgcgc cgtccagcgt gcggtcctac cttcgactca acacgcccga
       61 aatcttcaaa cggaatggac gcggtctcta tacgctgcgc gagcagggca tacaggaatc
      121 caggcccgtc ccttactcgc caccgagccg ccgggcccca ttgcccgcct atcattttgg
      181 ccgttcttcc ctgtatctgt gcgactgctt tccatggctg ctgcaacgcg gagcaaattc
      241 gatccacgcc gtcgtcaccg atcctcccta tggcctcttc gaatactcgg cggaacaaca
      301 acgaaagctt cgtgccggcc ggggcggagt atggcgcatt ccgccgtcat ttgacggaac
      361 gattcgttcg cccttgccgc ggtttaccgt tttgacggac agcgatctca gatagctgag
      421 cgattttttt ctcgagttcg gaaaagtgct cttggccgtt ctggtcccgg gcgccaacgt
      481 cattgtagcc agcaacccac tcctctcgca cgtcgtggcc ggtgccttgt cggcggtcgg
      541 gctcgagcgc cgcggagaaa tcgcgcgact ggtcacgacc atgcgcggag gagaccgtcc
      601 gaaggatgcg catgaagaat tttccgacgt aagcgtcatg ccacgttcga tgtgggaacc
      661 gtgggtgctg ttcagaaaac cgctcgaagg tcgggtgcag gataacctgc gcaaatggaa
      721 aacgggcggt ttcagacgac ccaccgggaa ccagccattt ggcgacgtga ttcggtccgc
      781 gccgacccac aagtccgaac gcggccttgc gccgcatccg agtctcaagc cgcagggatt
      841 cctgcgggcg ctggtccgcg gtgcgttgcc cctgggcgaa ggtgtggtcc tggatccgtt
      901 cgccggttcg ggttcgacgc tggcggccgc ggaatatgcc ggatatgaaa gcatcggcat
      961 cgaaaaagat gctcactatt tccgtcttgc aaaaaaggcc attcccaaac tttcggcatt
     1021 caagaacgga aacaactact cgctcaatct gtagacccag ttccggtgcg gattttcgat
     1081 gccttccctg tgcagagtcg cggtcctggt accaagttcg ccgcgcggat tcctgcgaaa
     1141 gtctgccccc atgacctgcc ccagatagac ttcacgaaag ctcatcggtc gtcgctcgcg
     1201 ggccggctcg gtttgattgt cgacgtcata cacgcacacg cacatccact ggtcgcgcgc
     1261 accatgcgtg tcgaccgcgc cgtcgtgctt gcgcgtcgtc tttatatcga cgccttccgt
     1321 tcctgccttg acgctgttgc gtggataaac tccggcaacg accaaatccg gatggccgtt
     1381 atggtaagaa ttgacgacga gcgtacggga gtgcttcgcg aggctcgacg tgagcatatc
     1441 cgagagcagg ccagagagga tcgccggtcg aatcgtgtca tcgagccgca tcaggccacg
     1501 ttcgacgagc ccacggttca cgtcgaaaaa gaaatcatac acgtcctgca ttgccatctc
     1561 gaagtcccgg agacgcaact cgtacggcag ctgtgccact gcattgaact gttgcgcgtt
     1621 gaccctgttc ctatgaatcg tcatggaaac tcctggatcg gctccgccgg caggacttcg
     1681 gtacgccccg gttcttcggt ctcgaagctg tagtcgacgg cggccgtgcg gtcgggaccg
     1741 cgcgcgacgc cgagacgatc gagtaataaa gttccggcga cggcgatggc gaggttcgcg
     1801 atcaccgaat aaagcgctgc atagccggga atgctgagag ggccgagcgg gagcggaaag
     1861 atcacgctcg tgaaatgctg cgtccaggcc atgccggtgc cgacgagcat gccggcgatc
     1921 cagccggcga tgagcgcgcg ggcatgaaac cagcgcccgt agagtccgag cacgacgctc
     1981 ggcagggtct ggatgatcca gattccgccg agaagttgaa actggatcgc gtactggttc
     2041 ggtagaaaaa tgatgaaggc aagcgcgccg atcttcacga gcagggaaac gatcttggca
     2101 atgcgtgcct cgccggcttc gtcgggcttg cggcgcgcaa ggtacggcca gagattgcgg
     2161 gtaaacagat tggccgcggc gatcgacatg atcgccgagg gaacgagcgc tccgatcgcg
     2221 atcgcggcga acgcgatgcc gacgaaccag ccgggaaagt tgtggaggaa gagcgccggc
     2281 acggcgaagt tcggtccgta ctggtgaaag tatccgctgt aagccggaag atcgccgact
     2341 ccggaagcat aggccatgaa gccgagcccg gcgatgagcg cgagcgcgag cgaataggcg
     2401 ggaaggagcg ccatgttgcg cttgacggtg ccgcggctgc gggcggatag aagtccggtt
     2461 accgcatgcg gataaagaaa cagcgcaagc gcggatccga gggcgagggt cgcataggcg
     2521 ctcatggcgc cgaggctgcc ctgcgaaggc ccagcgaggt agagcatctt ttccggaatc
     2581 ctcgcgaaga tcgcgccgaa gcctccgagg tgcagcggaa tcacgacgac ggcgacgatc
     2641 accgtgagat agatgagcgc gtccttgacg atcgcgataa gcgcgggcgc acgaatgccg
     2701 ctcgtgtaag tgtaggcggc gagcaccaga aaggcgatga cgagcggcat gtcggcgagc
     2761 cagccgtgcg tggtgaagcc gagcgctgca accacgacct ggatgccgac gagttgcagc
     2821 gcaatgtagg gcatggtcgc gaggattccc gtgatcgcaa cgagcgccgc gagcgtacgc
     2881 gaaccaaagc gcccatggac gaagtccgcc gcggtgatgt agccgtgccg gcgcgcgacc
     2941 gaccacagcc gggtgaacac cacgtacacg agcggaaaaa cgagcaaggt atacggcagc
     3001 gcgaaaaagc cgatcgcgcc cttcccgtag acgagcgcgg gcaccgcgac gaaggtatag
     3061 gcggtgtaga gatcgccgcc gagaagaaac caggtgacga cggtgccgaa gcgccgtccc
     3121 ccgagccccc actcctccag ccggtcgagc gggccgcggc gccagcgggc ggcaaagaac
     3181 ccgacaagcg ttacgagcgc aaaaagagcg acgaagacgc cgatcgccca gggctgcacg
     3241 ggctcagatc cgaagccgat aggcgagcgc cgtagcggcg cccgtcacga tgatccacag
     3301 gaactggaac cagtagaaaa aagggacccc gaaaagcgcg ggccgggcgc ggttgtagag
     3361 cggcacggcg agcgcgatcg cgcagcaacc gaggaagagg atcgcgatca gggtcttggc
     3421 gaacggaccg gcgtgtcttt ccggattcat gcgtccctcc gtgcacgttt tttagcacat
     3481 cgccgacgca gacacaagcg cgtcgaacgc ggctcaatgc ccgggaacgt cgccccagag
     3541 aagtttgtgg agctgcaact gaaagcgcac gggcaggcga tccgcgagaa tccattcggc
     3601 aagcgcgcgc ggggcgagtt cgcccgcgac cggcgagaac agcacttcgc agcgagcggc
     3661 gagatcgtgt tcacgcagga actctcgcgc ccattcgtag tcgccgcgct cggcgagcac
     3721 gaatttcgcc tgatcgcggg cgcgcagttg ctccagattt tccagacgat tgcgcgagac
     3781 ctcacccgaa ccgggcgtct tgacgtccat cacgataatc gcccgcggat cgagccccgc
     3841 gatcggcagg gctccgctgg tctcgatcga aatcgcgtag ccggccgcgg cgagtcgcgc
     3901 aacgagaccg cgacaattcg cctgcgcgag cggctcgccg ccggtgatgc agacatggga
     3961 gacggcgtag cgcgcgactt ctgcttcgat ggcatcgaaa ctccgccatt cgccgccgtg
     4021 aaatgcatac gcagtgtcgc accagcggca gcgcaaggga cagccggtga ggcgcacgaa
     4081 cacggtcggc cagccgacgc tcgcggcctc gccctggatc gaaaggaaga tttcggtgag
     4141 acgcaggcgc tcggccggcg gcaattcatc gcgccgcagc gagcgcatcg ggccagaagt
     4201 ttcgttcatc gcctcgagtc cgctgcgagt cctgtccggg gggatcgctt cggccgatcc
     4261 ggacctgcat tcctgccggg actccacgcg gcgcgacggc tcgggtgcga agcctggcgc
     4321 acaagtcccg gagttactgt cccggcgccc ccatctgttg caggcgctgc tgcgcgaggc
     4381 ttgccgcggg cgtgcccggg taatgcgaaa tgacgctctt gaacgtcgcc tgcgccccgt
     4441 tgtaatcctt cagtgcgtac tgggcatagc ccatcttcag ataggcatcg gaagcctttg
     4501 ccgaggaggg atacttcgtc accacctgct gaaagttgtc gatcgcatcc ttgtattcgc
     4561 cgttgacgta atgggtctcg cccatccagt accaggcgtt cggcacatag ctgctctgcg
     4621 ggtacttctt gatgaacgca tcgaaaccgc gaacggcggc cgagtaattg ccggcccgca
     4681 gttggtcgaa ggccgcctga taagcctcgg aatcggcacc ctgcgaaggc gtcggggtcg
     4741 cgctcgcatt cgccgaggca acgggcggcg cactcgccga agccgccagt gcgatcgggc
     4801 cggtcggaaa ataatttccg cccgccgaac ctgcgccgtt gccggcgccc gggccggcgc
     4861 cgacgccggg agtcgcgagc gtgcttccct gtccgagcgc ggcgaggcgc tgatcgaagt
     4921 cgcggttcac gagctgctga tgactttgca tctgctgcag acggtgctcg aggtcctgca
     4981 cctcaccctg cagagtggac aactgctcgc tcgactgctg ttgttgcgag gccatgttca
     5041 tcatcgcctg cccgccgacg acgtgttgca gccgatcgac cttggtctgc aactgatcga
     5101 tctgctgata caccggatcg tccggggaaa cggccgaaga gcagccgccg agcgcgcccg
     5161 ccgcagcgag cgcgcccagc gccaggagca tgcctgcctt catggggtgg cggtgggagc
     5221 gggcgtcgtc ccgaagacga tcacgacccg ccggttctgc gcccaggcgg cctcgttgtt
     5281 cgccgaatcg gcgggatagg cctcgccgta gctgatcgtc ttgatctggc tgtcgctcac
     5341 gccctgcagc tcgaggaagc gctgcacggc cacggcccgg cgcgaaccga gcccgatgtt
     5401 gtaatcccag gtgccgcgat catcgcaata gccctggagc acgacgctgg cgccggaatt
     5461 ggcgacgaga taggcggcct gtgccttgag aagcgggaag tattgcggct tgaccaccga
     5521 ttggttgaag tcgaaataca cggtgcgatt ttgatagctg ctctgcggac cggcctggat
     5581 cgcctgggcg ttgcccatgc ccgccccgag agcccccgcg gtcgaaccgg cagccggtgc
     5641 actgccggcc atcggcgcct tcttcgggcc gctggcgcag cccgcgagga cgagcatccc
     5701 ggcgaaagcg aatgtgacca aaaccttgtt cgtgttcctg ttcatggctt gactcctgtc
     5761 gttgggatgg aaacagacgc ccctttactt tacgccagtc gcgctcgggc tgccgggcgg
     5821 agtcggcgga aacgggcccc aggcgggctg ggaaagcccc ccgctgcttc ccgacagctc
     5881 ctcgcgaacc ccggtgttga ccgataccgt cgcgagcaca tgctgctcgc cgtgctggct
     5941 gccgtagagg atcagcgcgc cgttcggcgc gaagctcggc gagacgtcga gcggccccgg
     6001 cgtgagctcg cgcagattgc cgcttccccg atccatgacc gcgatcgtga gcgctccgcc
     6061 ctggtcgcga tgcacgaagg cgatgctctt gccgtcgggc gacaccgcgg cgcgcgcgtt
     6121 gtatttgccc tgccaggtga tgcgctcggc gccgctgccg tcgagcgcga tctgatagag
     6181 ctggggattg ccgccgcgat cggaagtgaa aatcagcccc tgcccgtttg gcagccaggc
     6241 gggctcggta tcgatcgcgg gattggccgt gatctgcgcg agcctgccgc tcgccagatc
     6301 catcacgtag atgtcgggat cggccggatg ggtcgaaagc accagcgcga gtttttgccc
     6361 gtcgggcgag aaggccggag ccccgttgat gccgggacgc gcgatcagct ccttgcgcgc
     6421 tccggtcttc acgtcctgga gatagatcgc gctgccgttc gcgccgtact ccacataggc
     6481 gatctgcgta ccgtcgggcg accaggcggg agacatgatc agatcgttgg ccgtgaccac
     6541 ggtctgcgta ttggcgccgt cggcatcggc aatgaccagt tcccagcggg tacgtccctt
     6601 gcccgcgtct tcgtttctga cataggcgat gcgcgtcgcg aaggctccgc gaatgccggt
     6661 gagccgctga tagatcatgt cggccgcgac atgcgcggtg aagcgcagat tgtccgccgg
     6721 ccccggcagg aggtagccga gaagctgctg ttcggtgtac acgttgaaaa gctggaagcg
     6781 aaccctggcc tggtcgttcg cgacattgac gccgccgatg acgaggtcgt tcacgctgag
     6841 agccttccag tcggtgaagt tgatgtcgtt cgccgcgctc ggacgcgaaa tcatgctcgc
     6901 gcgcggcagc ggcgcgaaga gcccgctgcg ctcgagatcg tcgccgatga ccttggcgac
     6961 atccaccggc gccggcccga gcccctgcca ggcgaaggga acgaccgcga tcggcgtcgg
     7021 ctgactgaag ccctgagtga tcttgatgac gagatcggcg cgcgccggaa cgaacgtgag
     7081 cacgagcgcg agcacgccgg cgaaaagaag ggcgatcgaa tgtttcattg gctgtctcct
     7141 gcctcagggc ccgggattga attcgaaggt gatcgtgcgc tgaaagacgc tcggatcatc
     7201 ggggatcgga agtggcgagg atttcaggat ggccgtttgg acggattgcc gtaccgccgc
     7261 atcgccgttg cattgctgca cgaccgcgct caccacctcg ccgtcgggaa gctgcgtaat
     7321 cttgacgaaa caatcgagcc ccggcggcgt cgagggcggc cggttccaat tgctgttgac
     7381 cttggccttg attgcggccg tccacaggcc cagcgcggcc gcgttacgct ggttctcctc
     7441 cgcctgaatc tgcgcctgca actgcgcctt gcgttgggct tcggcctgcg ccgcggcctt
     7501 ggccttggcc tccgccgcgg ccttttccgc ggcttcccgc tgatcctgga tcttctgcag
     7561 tcgcgccgct tcttcctcct gcgccttctt ggccgccgcc gtctgggcct gcaactcttg
     7621 cagtttcttt tgttgggcct ccttcgccgc cgccgcctgc ttcgcctgct ccgagacctg
     7681 ctggagttgc tgctgctgct ctgcggcggc cgtcgccgcg ctctgcttct gctggcgcag
     7741 atccgcgagc tgctgttcgg tcttctcgcg cgattggcgc gcgtcctccg cttccttctt
     7801 cagccgttcg acttcctcgg tgcgtgcctg ctgggtctgg gcgatctcct gctgcgcggc
     7861 ctgataatcg gactcgttga cgaccacggc cttgatcggc tgcggctcgt tgccgggtcc
     7921 gggcggcgcc tgcggattga tggcaccgcc gcgcacgccg acgaagacga gcacgccgac
     7981 gatgacgtgc agcagcaggg cgagcgcaaa gggcacggca ttgcgccgca gccgcgagga
     8041 atcggccgac ctcatggttg ccccggtttc ctgacgagcg gcgaactcgg cgtcttggtg
     8101 atgaggccga tgtttttcgc gcccgcggat tgaagcagcg tcatgccgcg cacgacgtcg
     8161 gcgtagctcg ccgcctcgtc gccacgaaca agcaccggag tatccggagt ctgcctcagg
     8221 acgaccgtca ccatcgcgac gatcatgtcg ggcgagagcg gcgcgtcggg atgggcgccg
     8281 atgttgagat agagcctgcc gccgggatcg acgctgagta ccaccggttg atggcgttcc
     8341 atctccttct cggacagcgc ctgcgccgcg gcctgcggca gattcacctt gaccccgcgc
     8401 gtcaagagcg gcgcggtgat catgaagatg atcaggagca ccagcgtcac gtcgatgtag
     8461 ggcacgacgt tgatctgcga catcaggcga cggcgccggc tcatgacgtg gcgtcgccct
     8521 cggccacccg atggtgctcg agcacgttgg tgaactcctc cgtgaagctc tcgtatcgtc
     8581 cgtcgaggcg gtccacctga ttggcgaagc ggttatagaa aatggtcgcc gggattgcgg
     8641 cgatgagtcc catcgcggtc gcgatcagcg cctcggaaat gccgggggcg accatcgcga
     8701 gcgtcgcctg ctcggcgttg ccgagcccct gaaaggcggc catgattccc cagaccgtgc
     8761 cgaagagtcc gacgtagggc gccgtcgatc cgacggtcgc gagatagccc atgctggttt
     8821 cgagccgatc cagttcgcgc gagcgggtcg cggccatggc gcggcgcgca ttggcgagtg
     8881 cgttggccgc gcccggatgc ccgtggcgga tgagccgggc gaactcgcga aaacccgagc
     8941 ggaacatctc ggcgaggccg ccgccgctcg cgcgcgaatc gagccgcttg taaagctctc
     9001 cgaggtccgc tcccgaccag aactcgccct cgaacttcgc ggcgttcgcc tcggcgcgct
     9061 tgagttccgc gtatttgcgg aaaatcaccg cccaggagaa gatcgaggcg atcacgagaa
     9121 tcacgagcac cgcctgcacg acgatgctcg cgctcgcgat catgctgtaa atggaaaagt
     9181 tggtgctcat gaagccatcc tggcgagaag tatgggagga agtgcacggg cgcgaaagct
     9241 gggacggtcc acgcaggcta tttcgaccga ggccacgacg agtggttcag cggccgcgcc
     9301 agcacgcgcg accttctgct cgcagacgaa gcgggttttt gtcgcagatg tgagcgcgac
     9361 gctgatttcg aggagatcat tgaaatgagc cgggcggcga tagcgtatat ccacccgcgt
     9421 aacgacgaag aggatctcgg tctcgcgcat gagcgtgtcc tgctcgatcc ctcccgcacg
     9481 cagccattcg gtgcgcgcgc gctcgaggaa cttcacgtac tgcgcatgct gcacgacgcc
     9541 gcccagatcg gtgtcctcgt agtacacgcg caccggccag acgaagggct tcacggcctc
     9601 attcatcgcc tgctcccgcg aagagatccg catcgcgcgc cggcggagcg agacccagat
     9661 gctgccaggc gcgcgcggtt gcgacgcgcc cgcgcggcgt gcgcttgagg aaaccctgct
     9721 ggatcaggta cggttcgatc acgtcctcga tggttccgcg ctcttccccg agcgaagtcg
     9781 cgaggctgtc caccccgacc gggccgccgt cgaagtgttc gatcacgatc gcaagcaggc
     9841 gccggtcgag tgcatcgaag ccgcgccggt ccacttcgag gaaatcgagc gcggcgcggg
     9901 cgacctcctc ggtcacggta ccgtcggcgc gcacctcggc gtaatcgcgc acccggcgca
     9961 gcagacgatt gacgatgcgc ggcgtaccgc gcgcccgccc ggcaatctcg tgagcgccgg
    10021 ccggctccag ccgcacgccg agaattcccg ccgaacggcg cacgatcgcc tccaatgccg
    10081 ccggatcata gaagtcgagc cgcgagacga tgccgaagcg atcgcgcaat ggcgaggtga
    10141 gaagcccggc gcgggtcgtg gctcccacga gcgtgaaggg cgcgagatcg aggcggatgg
    10201 agcgtgcggc ggggccttcg ccgatcatga tgtcgaggac gaaatcctcg agcgccggat
    10261 agagcgcttc ttccacgacc ggactgagac ggtggatctc gtcgacgaag agcacgtcgc
    10321 cgcgttcgag attggtgagg atcgcggcga ggtcgccggc gcgctcgaga accggccccg
    10381 aagtctgatg aagtgtcgcc cccatctcgt gggcgatgat atgtgcgagc gtcgtcttgc
    10441 cgaggccggg cggaccgaac agcaggcaat gatcgagcgt ctcgccgcgc ccgcgcgccg
    10501 ccgcaatgaa aatctccatg cgctcgcgca cctgatcctg gccgatgtac tccgccaggc
    10561 gctgcgggcg caggcgcggt tcctcgcgca gctcgccggc ctc
//
LOCUS       DASUJG010000393         3997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_601505, whole genome shotgun sequence.
ACCESSION   DASUJG010000393 DASUJG010000000
VERSION     DASUJG010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601505"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1417
                     /locus_tag="VFX38_08395"
     CDS             <1..1417
                     /locus_tag="VFX38_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEX5314905.1"
                     /translation="ADCKAAPTNQTAAECGYSVIYALAPSPAKRGVIWAGTDDGLVWR
                     TEDGGKHWHDVTPKGLADWSRIDTIEADPHDASSAYAAVDRHQVDDFAPYMYVTHDAG
                     VHWRLAVAGIPRGDYVRVVRTDPERKGLLYAGTEQGVFVSFDDGRHWQSLQLNLPTAS
                     VHDLVVHGGDLVAATHGRGIWILDDIEPLREASAKVAKSAVHLYRPRPAYLFPLSLYH
                     AEARPPEEPHAANPPTGAIIDYRLAQSAKGAVQLAIYDANGRLVRRYSSAAKPTKMPL
                     ANFPDYFKAPPTVLPATAGLNRFVWDFHYAPPLGVDPYWAGPAVLGRTPRGPLGPLAP
                     PGTYRVVLTVNGAEYSAPLELIADPNLHAAAETLRANAAFALELEDALNRNAGLLARA
                     EEAAKAADKTGDVARAKRIRAVLAQFDLDSVNGQLYGLMREVSSSAEAPTASAKADSA
                     ALEHKSERARAALAALLVPGM"
     gene            1494..2225
                     /locus_tag="VFX38_08400"
     CDS             1494..2225
                     /locus_tag="VFX38_08400"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5314906.1"
                     /translation="MATRDSHIPGGRVTLDSGLRCWVAKPAENAAPPGVLLYIEAYGV
                     NAHMRAVADRFAQAGYAAIIPDIFHGRTFDYGNTQSAMRAVRELDDAQVMRESAEALK
                     WLAEDGHGKPAVVGFCMGGRLAFLAAIELGSHISRAVCYYGGGIAAEGERDKYGRRPP
                     IVGVADIAVPLLLHYGGRDAVIDSAEQLRVATALSKAGKQYVLAVYPEAGHAFNCEDR
                     PHYHDASAAEARRLTLAFLGGPHRH"
     gene            complement(2273..2977)
                     /gene="hda"
                     /locus_tag="VFX38_08405"
     CDS             complement(2273..2977)
                     /gene="hda"
                     /locus_tag="VFX38_08405"
                     /inference="COORDINATES: protein motif:HMM:TIGR03420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEX5314907.1"
                     /translation="MRQLGLPGLVLAEAPSLESFWPGANGDAAAAVQQMADGARIPMV
                     FLHGPGGVGKTHLLKAAWRRAQEAGRRGGYLPLEQALMLDPALIEGWGELDFVALDGI
                     EHVAGFALWQRALFRLSEELRERDAGLLVAARHPPDALGLELVDLASRLAWGPVYALA
                     PLDDADLAALAGHLARTRGLKLPDAVANFLVRRIARDPQHVALAIARLDEAALAAQRR
                     LSIPFARSVLLDETKA"
     gene            complement(2977..3600)
                     /locus_tag="VFX38_08410"
     CDS             complement(2977..3600)
                     /locus_tag="VFX38_08410"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX5314908.1"
                     /translation="MRFLPNALSIVRMALVAPVVWCIATHRYEVALGLFAVAGVSDAL
                     DGAIAKRYGWATVLGAFLDPAADKLLVLGSYTALAVDLQVPWWLLGLLFGRDLVIVGG
                     AAVYRWRRGPFEIQPALTSKIATGLLIAIGLALVIQGAYPYLGASAKVALIVLLAVSL
                     VVSGADYVLTWGLRFARLREPVRPELTAVPSAQAVQTMERKSATGPS"
     gene            complement(3600..>3997)
                     /locus_tag="VFX38_08415"
     CDS             complement(3600..>3997)
                     /locus_tag="VFX38_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314909.1"
                     /translation="TPFSATAATPQAAARAAVEHAATLLSRQLAYVPQQGSGGTLSVT
                     VSGVASLAAEAAVARLLANVPGVSDAHLASIDADRADFSLRYAGAPSELARALSLSGT
                     LTPEQAAPSAPASGTSAASSGTLYFRYTP"
BASE COUNT          716 a         1356 c         1343 g          582 t
ORIGIN      
        1 cgccgattgc aaggccgcac ccacgaatca aactgccgcc gaatgcggct attccgtgat
       61 ctatgcgctc gctccctcgc cggcgaagcg cggcgtgatc tgggccggaa ccgacgacgg
      121 gctcgtgtgg cgcacggaag acggcggcaa gcactggcac gacgtcaccc cgaagggact
      181 cgccgactgg agccggatag acacgatcga agcggacccg catgacgctt ccagtgcgta
      241 cgcggccgtg gatcgtcatc aggtggacga cttcgcgccc tacatgtacg tgacgcacga
      301 cgccggagtg cattggcgac tcgcggtcgc cgggattccg cggggcgact acgtgcgcgt
      361 cgtgcgcacc gatccggaac gcaagggatt gctgtacgcc ggcacggaac agggcgtctt
      421 cgtctccttc gacgacggtc gccattggca atcgttgcaa ttgaatctgc ccaccgcgtc
      481 ggtgcacgac cttgtggtgc acggaggcga tctcgtcgcc gcgacgcacg ggcgcgggat
      541 ctggattctc gacgacatcg agccgttgcg cgaggcgagc gcaaaagttg cgaagagcgc
      601 ggtgcatctg taccggccgc gccctgcgta tctctttccc ttgagcctgt atcacgccga
      661 agcacgtccg ccggaagagc cgcacgccgc aaatccgccg accggtgcca ttatcgatta
      721 ccggcttgcg caaagcgcga agggcgcggt gcagttggcg atttacgatg cgaacggcag
      781 gctggtgcgc cgctattcga gcgccgccaa gccgacgaag atgccgctcg caaacttccc
      841 cgactacttc aaggcgccgc ccaccgttct gccggcgacg gcgggcctga atcgcttcgt
      901 gtgggatttc cactacgcgc cgccgcttgg cgtcgatccg tactgggcgg ggccggcggt
      961 gctcggccgc acgccgcgcg gcccgctcgg gccgctggcg ccgccgggaa cgtaccgcgt
     1021 cgtgctcacg gtgaacggtg cggagtattc cgcgccgctc gaattgattg ccgacccgaa
     1081 cctgcacgcc gcggccgaga cattgcgcgc caatgccgcc ttcgctctcg aactggagga
     1141 tgcgctcaac cgtaacgcag gtctgctcgc ccgcgccgag gaggcggcga aagccgccga
     1201 caagaccggc gatgtcgcac gcgcaaaacg gatccgcgcc gtactcgcac aattcgacct
     1261 cgattccgtc aacggtcaac tctacggcct gatgcgcgaa gtctcgtcga gcgccgaagc
     1321 gccgacggca agcgccaaag ccgactccgc cgcgctcgag cacaaaagcg agcgagcacg
     1381 tgcggcgctc gccgccctgc tcgtcccggg catgtagcaa gcggagcgat tgcgcgataa
     1441 tcgtgccgat ggcaggagcg agcctgctcc tacccatgga tcgggggtgt caaatggcca
     1501 cgagagactc acacatcccg ggcggccggg tcacgctcga ttccggactg cgttgctggg
     1561 tcgcgaaacc agccgagaac gccgcgccgc ccggcgtcct gctctacatc gaggcctacg
     1621 gggtgaatgc gcacatgcgt gcggttgccg atcgcttcgc gcaggccggt tacgcggcga
     1681 tcatccccga cattttccac ggccggacat tcgactacgg caatacgcaa agtgcgatgc
     1741 gggccgtgcg cgaactcgac gacgcgcagg tcatgcgcga aagcgccgag gcactcaagt
     1801 ggctcgcgga agacgggcac ggcaagccgg cggtggtcgg cttctgcatg ggcggacgcc
     1861 tcgcattcct cgccgcgatc gaactcggat cgcacatctc gcgggcggtt tgctattacg
     1921 gcggcgggat cgcggccgag ggcgaacggg acaaatacgg gcgcaggccc ccgatcgtcg
     1981 gcgtagccga tattgcggta ccgctgctgc tccattacgg cgggcgcgat gcggtgatcg
     2041 actccgcgga acagcttcgc gtcgccaccg ccctgtcgaa ggccggcaaa caatatgtgc
     2101 ttgcggtcta tcccgaagcg ggccacgcct tcaactgcga ggatcgcccg cactatcacg
     2161 acgcgagcgc ggccgaagcg cggcgactga cgctcgcctt tctcggcggc ccgcaccggc
     2221 actgatgggc ctgcctgaca ccaactccgc gcgcacacgc gactgcgcga aatcaggcct
     2281 tggtttcgtc cagcagcacc gagcgggcga aggggatgct caatcgtcgc tgggccgcga
     2341 gcgcggcttc atccaggcgc gcgatcgcca gcgcgacatg ttgcggatcg cgggcgatgc
     2401 ggcgcaccag aaagttcgcg acggcatcgg gcagtttcag tccccgcgtt cgcgcgagat
     2461 gcccggcgag cgccgcgagg tccgcatcgt cgagcggcgc cagcgcatac accggtcccc
     2521 aggcgagccg cgaggcgagg tccaccagtt cgaggcccag agcatcggga ggatgccttg
     2581 cggccaccag caagcccgcg tcacgttcgc gcaattcttc gctcaggcga aacagggcac
     2641 gctgccagag cgcgaatccc gcgacgtgtt cgatcccgtc gagagcgacg aagtccagtt
     2701 ctccccagcc ctcgatcagg gccggatcca gcatcagcgc ctgctcgagc ggcagatatc
     2761 ctccgcgccg gccggcttcc tgcgcccggc gccaggcggc cttgaggaga tgcgttttgc
     2821 cgacgccacc cggcccgtgc agaaaaacca tgggaatgcg cgcgccgtcg gccatctgct
     2881 ggaccgcggc ggcggcgtcc ccgttcgcgc cgggccagaa gctctccaga ctgggagcct
     2941 cggcgaggac caatcccggc agaccgagtt ggcgcatcag gaaggcccgg ttgcgctttt
     3001 ccgctccatc gtctgcacag cttgcgccga gggcacagcc gtgagttccg gccggacagg
     3061 ttcgcgaagc cgggcgaagc gcagacccca ggtcagcaca tagtcggctc cgctgacgac
     3121 gagcgaaacc gcgagcaaca cgatgagggc gaccttcgcg ctcgccccga gatagggata
     3181 ggcgccctga atgacgagcg cgagcccgat cgcgatcagg agcccggtcg cgatcttgct
     3241 cgtcagtgcc ggctgaatct cgaagggccc gcgccgccag cgataaacgg cggccccgcc
     3301 gacgatgacg agatcgcgtc cgaacagcag gccgagaagc caccacggca cctgcagatc
     3361 gaccgcgagc gccgtgtagc ttccgaggac caggagcttg tccgccgccg gatcgagaaa
     3421 tgcgcccagc acggttgccc agccgtagcg cttggcgatg gccccgtcca gcgcatcgct
     3481 gacgccggcg acggcgaaca atcccagcgc tacctcataa cgatgggtcg cgatgcacca
     3541 gacgaccggg gccacaaggg ccatgcgcac gattgacaga gcattcggca gaaagcgcat
     3601 cagggcgtat aacgaaaata cagggtgccg gaggaggccg ccgaagtccc ggaggccggt
     3661 gcgctcggcg ccgcctgctc cggtgtcaga gttccactga ggctgagagc gcgcgcgagt
     3721 tcgctcggtg ctcccgcata acgcagggag aagtcggcgc gatcggcatc gatgctggcg
     3781 aggtgagcgt ccgaaacgcc ggggacgttc gcgagcaaac gcgcgacggc cgcctccgcg
     3841 gcgaggcttg cgactccgga caccgtcaca ctcagagtgc ctcccgaacc ctgttgcggg
     3901 acataggcga gctggcgcga gagcaaggtc gcagcgtgct cgacggcggc gcgcgcagcc
     3961 gcctgcggag tcgctgccgt tgcggaaaag ggtgtgg
//
LOCUS       DASUJG010000394         2689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_846270, whole genome shotgun sequence.
ACCESSION   DASUJG010000394 DASUJG010000000
VERSION     DASUJG010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846270"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1002)
                     /locus_tag="VFX38_08420"
     CDS             complement(139..1002)
                     /locus_tag="VFX38_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010379763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX5314910.1"
                     /translation="MAQAIWTGHISFGLVNIPVALYSAERKTDLSFRMLDSRNRARIR
                     YERINDETGDEVPWDKIVKGFEFEKGNYVLLDNEDFERAAPEATQSIEIESFVELAAI
                     EYAYFERPYVLEPESHGEKGYVLLRDTLRETGRAGIAKVVIRTRQHLAALVASERALI
                     LNLLRFHQELRSAEDFKLPDEEKKGFRISAAEKKMAKQLVESMSGEWKPDFWHDDYRA
                     ALLEYIEQKARKGKVAAEPKAKPRKKAQVIDLTERLRKSLEQSGGRKGGASKKTAARK
                     RSGAKRKAASR"
     gene            1216..2454
                     /locus_tag="VFX38_08425"
     CDS             1216..2454
                     /locus_tag="VFX38_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX5314911.1"
                     /translation="MSDREKPQAPDDRAPLSGLRVLDLSRVLAGPWCTQLLADLGAEV
                     IKIERPGGGDDTRSWGPPFAGPPADGLSAYFLCANRNKRSVAVNFKHPRGAELLRRLA
                     GCCDVLIENFRPGALAASGLDYESLHTEFPRLVYCSISGFGQGGPDSQRGGYDLLIQA
                     MGGLMSLTGKTDGEPMKVGVALTDILTGLYASTGILAALRQRDASGEGQHIDLALFDV
                     QVAALANQALNYLVSGRVPKRIGNAHPNIVPYQAFAARDGHLIIAVGNDSQFRRFCET
                     AGLPELVGDSRYATNAAHVSNRKELLARIEPVIASRRRDEWIAALEEADVPCGSVNDL
                     AEVFAAPQLEARGMRFTFEAGDTSLPQIASPLHLSAAPQVRGSAPPRLGADTCAVLAE
                     LLGLEDGELETLHAAGAIQS"
BASE COUNT          453 a          934 c          769 g          533 t
ORIGIN      
        1 ccgccggctt ttcggcgagt tcgcgctccc agccgacgat gccgccgagc accatggcgg
       61 cagcgatttt cccgacgaaa atgagttgga tgcgccagtc gtgcattcaa cccgctccgc
      121 accaagttcg cgaccggttc atcgcgaagc cgctttgcgt ttcgctccgc ttcgtttgcg
      181 agcagccgtc ttcttgctgg cgccgccttt ccttccgccg gactgctcga gacttttccg
      241 cagccgctcg gtgaggtcga tcacctgcgc cttcttgcgc ggtttggcct tcggttcggc
      301 tgcaactttc cccttgcgcg ccttctgttc gatgtactcg aggagcgccg cgcgatagtc
      361 gtcatgccag aagtcgggct tccactctcc gctcatcgat tccaccaact gcttcgccat
      421 tttcttctcc gctgcggaaa tcctgaaccc cttcttctct tcgtccggaa gtttgaagtc
      481 ctccgcgctc ctcagttcct gatggaaacg caggagattc aggatcaggg cccgctcgct
      541 tgcgaccagc gcggccagat gctgacgcgt gcggatcacc accttggcga tgccggcccg
      601 gccggtttcc cggagcgtgt cccgcagcag cacgtagccc ttttcgccgt ggctttcggg
      661 ttcgagcaca tacggtcgct cgaaatacgc gtattcgatc gcggcaagct cgacgaaaga
      721 ctcaatctcg atgctttgcg tcgcctcggg cgccgcgcgc tcgaaatcct cgttgtcgag
      781 cagcacgtaa ttccccttct cgaactcaaa tcccttgacg atcttgtccc agggaacttc
      841 gtcgccggtc tcgtcgttga tgcgttcgta gcgaatgcgc gcgcggttgc ggctgtcgag
      901 catccggaag ctgaggtcgg ttttccgctc ggccgaatag agcgcgaccg ggatgttgac
      961 gaggccgaag gaaatatgac ccgtccagat cgcctgtgcc atggttgcgt tctcctctgc
     1021 aatcgcttgc ggaatccgtc cccgcgtcga tcagaccgct gctttgactt cctcgtggtc
     1081 cgctgcgagt tcgcaacgaa cgagcgccac cgtcagaata gcgccgcgga cgcgaccatt
     1141 tcaacgggca gcggcacact tgcacgaaac cacgccgacc gggacaattg gcctttgcgc
     1201 cgcgcaggtc ggacgatgag cgatcgagag aagccgcaag cccccgacga tcgggctccc
     1261 ttgtccggcc tgcgcgtgct cgatctctcg cgggtgctgg ccggcccctg gtgcacgcaa
     1321 ctgcttgccg atctcggagc cgaggtgatc aagattgagc gccccggcgg cggcgacgac
     1381 acccgcagtt ggggcccgcc cttcgcggga ccgcccgccg acggactctc ggcctacttc
     1441 ctctgcgcga atcgcaacaa gcgttccgta gcggtgaact tcaagcatcc gcgcggagcg
     1501 gagttgctgc gccggctcgc cggatgctgc gacgtgttga tcgagaactt ccgaccgggc
     1561 gcgcttgccg ccagcggcct cgactatgaa tcgctgcata ccgaattccc gcgtctcgtg
     1621 tactgctcga tcagcggttt cggacagggc gggcccgaca gccagcgcgg cggctacgat
     1681 ctcctcattc aggccatggg cggcctgatg agcctcaccg gcaagacaga tggcgaaccc
     1741 atgaaggtcg gcgtcgccct caccgacatc ctcaccgggc tctacgcttc gaccggcatt
     1801 ctcgccgcct tgcgtcagcg cgatgcgagc ggcgaagggc agcacatcga tctcgccctc
     1861 ttcgacgtgc aggtcgcggc gctcgccaat caggcgctca actatctcgt gagcggccgc
     1921 gttccgaagc gcatcggcaa tgcacacccg aacatcgtgc cctatcaggc ctttgccgcc
     1981 cgtgacggac acctcatcat cgccgtcggc aacgattcgc agttccgccg cttctgcgag
     2041 actgcgggat tgcccgagct cgtcggcgat tcgcgctacg ccaccaacgc cgcacacgtc
     2101 tccaatcgga aggaattact cgcgcggatc gaaccggtga ttgcatcgcg ccgtcgcgac
     2161 gagtggatcg ccgcactgga agaggccgac gttccctgtg gctcggtcaa cgatctcgcc
     2221 gaagtcttcg cagcgccgca actcgaagcg cgtggaatgc gcttcacgtt cgaagccggt
     2281 gacacaagcc ttccccaaat cgcgagcccg ctccatctga gcgcagcgcc gcaagtccgc
     2341 ggcagcgcgc cgccgcgcct cggcgccgac acctgcgccg ttctcgccga acttctcgga
     2401 ctcgaagatg gcgaactcga aacgctccac gccgccggcg caatccagag ttgaggacgg
     2461 cccgcactcc gcctttcgca taagaaaaaa ttctagttcc gactcccgct attttccggg
     2521 gggattaccg tagccgatcg ccgcatatgc catatttttg tacatccaat ttcccaataa
     2581 ttgtggtctg tcccctattc cccccacgca gcacccgcca ggtcttcacc gccccgctgt
     2641 ggccgcgatg acggacatga acacggcgaa tctgctccaa caagcgcgc
//
LOCUS       DASUJG010000395         3884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_725032, whole genome shotgun sequence.
ACCESSION   DASUJG010000395 DASUJG010000000
VERSION     DASUJG010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725032"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="VFX38_08430"
     CDS             <1..996
                     /locus_tag="VFX38_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018948022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX5314912.1"
                     /translation="EATGREPGFLIGGLPENFAAPVRLGRGECFVIEADEYDTAFFDK
                     RAKFVHYRPRVLVLANLEYDHADIYPDLAAIERQFHHLVRTVPGSGRIIVNGGAAALA
                     RVLKMGCWTPVDYFGEGPQASLAGQSIAPDRFELWVDGKPKTTVRWNLRGAHNMENAL
                     AALAAVRALGVPLDEALAVLPGFRGVRRRLTLLAETNDICLYDDFAHHPTAISRTLAG
                     LAAPGRRLVVAFEPRSRSLRAGVNAEGLAVAFAQADRVFVFRRADLAWDPATVLKSLG
                     AVLTVHSDIEALAAQLLAEARPGDDIVLMSNGGFGGLPARLQAALGNGGKISSKF"
     gene            1501..3000
                     /locus_tag="VFX38_08435"
     CDS             1501..3000
                     /locus_tag="VFX38_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEX5314913.1"
                     /translation="MNDAGVAAGKSWLGHPRGLATLFFIEMWERFSFYGMRALLVLFM
                     VAAVEKGGLGFDDRTATAIYGLYMAAVYLTSLPGGWIADRLIGQQRAVFYGGIVIALG
                     HFTLAIPRRETFFIGLILIVLGTGLLKPNASTMVGDLYPEGGARRDAGFSIYYMGINL
                     GGFIGPLVCGYLGQKINWHYGFAAAGVGMVLGLVQYRLTLHHLGDAGRHAQRRFDDAA
                     AEWRSRRRGWTWLASGLALLGLLIALGYVGAIVYPPAALALWSGFVILALGVGFVVWV
                     FFRGALDGRERRRLVALAILFLVQVVFWAGYEQAGSNFNLFAERYTDLSAFGWSFPAS
                     WFQSENSLLIILLAPFFAWLWIWLGRRRLDPSTPLKFALGMIFMGIGFVVMTGAAWVV
                     VAGHEALPTWLALTYLLHTIGELCLSPVGLSATTKLAPRRYGGRMMGTWFLFLALGEL
                     LAGLIAGRFSAKDVQDMPHIYLTIAAVGIGVGIVLAIFSRLIQRRLIEK"
     gene            complement(3075..>3884)
                     /locus_tag="VFX38_08440"
     CDS             complement(3075..>3884)
                     /locus_tag="VFX38_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEX5314914.1"
                     /translation="NAGDDGLAAGHLEFPIVGPIMIPDAPRAPEPRMRRARITAALRA
                     ARKTGAGILAARCLCCNARGAGALCPSCFRDLPWNDHPCENCATPLPDLPGTRCGACA
                     RRAPLYDAATAAFVYAWPVNRLLQDFKFRGRLPVGRILALALAEYLDLHQLPRPDVLV
                     PVPLHRGRLAGRGFNQAAEIARILAQSLDVAAEPALLSRARPTPPQLGLPRRERRRNL
                     RGAFACRRALAGVHVAIVDDVITTGSTAEAAAQVLKRAGATRVDVYALARA"
BASE COUNT          583 a         1231 c         1315 g          755 t
ORIGIN      
        1 gaggcgacgg gacgagaacc cggctttctg atcggaggct tgccggagaa ctttgcggcg
       61 ccggtgcggc tcggtcgcgg cgaatgcttc gtgatcgagg ccgacgagta cgacaccgcg
      121 tttttcgaca agcgcgccaa gttcgttcac taccgtccgc gcgtgctggt gcttgccaat
      181 ctggaatatg accacgcgga tatttatccc gatctcgcgg cgatcgagcg ccagttccat
      241 catctcgtac gcacggtgcc gggctcggga cggatcatcg tgaacggcgg tgccgccgcg
      301 ctcgcgcgcg tgctcaagat gggttgttgg acaccggtcg attacttcgg cgaaggcccg
      361 caggcgagtc tcgccggtca gtcgattgcc cccgaccgct tcgagttgtg ggtggacggg
      421 aagccgaaga cgaccgtacg ctggaatctt cgcggcgcgc acaacatgga aaatgcgctt
      481 gcggcgctcg ccgccgtgcg cgcgctcggc gtgccgctgg acgaggcgct cgcagttctt
      541 cccggctttc gcggcgtgcg tcgccgcctg acccttctcg ccgagacgaa cgacatctgt
      601 ctctacgacg acttcgcaca tcatccgacg gccatttccc gcaccctcgc cgggctcgcg
      661 gctccggggc gccgccttgt cgtcgcgttc gagccacgtt cgcgctcgct tcgcgccggc
      721 gtcaatgcgg aaggattggc ggtcgcgttt gcgcaggccg atcgcgtgtt cgtgtttcgg
      781 cgcgccgatc tcgcctggga tccggcgacc gtgctgaaga gtcttggcgc cgttctcacg
      841 gtgcactccg acatcgaggc gctcgccgcg caactgctgg cggaagcgcg tccgggcgac
      901 gacatcgtgc tcatgtcgaa cggcggcttc ggcggccttc cggcacgcct gcaggcggcg
      961 ctcggcaatg gcgggaaaat ttcaagcaaa ttttgattcg caaaaggttt cggttttgcc
     1021 aataccggcg acttgggaat ggcagatcaa tgccgggcca gacggcggcg gctacacgtt
     1081 gtgcattcag cacccgtgat gtgcgccgcg acaaatcgtt tgggcaggag ccggccaatg
     1141 tagggaacgt gctggacagc tcacgggtgc tcgcctgtcg aggtgtgcgt acggacgcct
     1201 gacgggttca cggcgcggcc gtttgtatct gcggcacttc ccgattcctg tgcacgcgcc
     1261 ggactgaact tgggttagtg gcgataacaa cagcgctttg cggcgttgcg gtcgtcgggc
     1321 ggtcgtgact tgacggaccg atattccgat gtttcaatgg cgccattcgg aaccaagaca
     1381 acgggccgtg aaagtccttg cgcgtccaat cctgctgggt ggatacggtc tggatagatg
     1441 cgcttcggtc gttgattgtt tgccgcttcg taccgcatcg ggacgcccgg ccgaaagccg
     1501 atgaacgacg ccggcgtcgc ggccgggaag agctggctcg ggcatccgcg cggactcgcg
     1561 acgctgtttt tcatcgagat gtgggagcgc ttcagcttct acggcatgcg cgcgctgctg
     1621 gtgctgttca tggtcgcggc ggtcgagaag ggcgggctcg gcttcgacga ccggaccgcg
     1681 acggctatct acggcttgta catggcggcg gtgtatctca cctcgcttcc cggcggctgg
     1741 atcgcggatc gtttgatcgg tcagcagcgc gccgtgttct acggcggcat cgtgatcgcg
     1801 ctcgggcatt tcacgctcgc gatcccgcgg cgcgaaacgt ttttcatcgg actcattctc
     1861 atcgtgctcg gcacgggact cctcaagccg aacgcgagca cgatggtggg cgatctctat
     1921 cccgagggcg gtgcccgccg cgacgccggc ttttcgatct attacatggg catcaatctc
     1981 ggcggcttca tcggtccgct cgtctgcggc tatctcggac agaaaatcaa ttggcattac
     2041 ggattcgccg cggccggcgt cggcatggtc ctcgggctcg ttcagtaccg cctcaccctg
     2101 catcatctcg gcgatgccgg gcggcatgcg cagcgccggt tcgacgacgc cgccgccgaa
     2161 tggcgctcgc gacggcgcgg ctggacatgg ctcgcctcgg gactcgcact tctcggcctg
     2221 ctcatcgcgc tcggctatgt cggagcgatc gtctatccgc cggccgcgct cgcactctgg
     2281 tcgggattcg tgattctcgc gcttggagtc ggctttgtcg tctgggtgtt cttccgcggc
     2341 gcgctcgatg ggcgcgaacg gcgccggctc gtggcgctcg ccattctgtt cctggtgcag
     2401 gtcgtcttct gggccggcta cgagcaggcg ggctcgaatt tcaacctctt cgccgaacgc
     2461 tacaccgatc tttccgcctt cggctggagt ttccccgcct cctggttcca gagcgagaac
     2521 tcgctgctga tcattctgct cgcgccgttt ttcgcctggc tgtggatctg gctcgggcgg
     2581 cgccggctcg atccctcgac accgctcaag ttcgcgctcg gaatgatttt catgggcatc
     2641 ggcttcgtcg tgatgaccgg cgccgcctgg gttgtcgtcg ccgggcacga ggcgctgccg
     2701 acctggctcg ccttgacgta tctgctgcac acgatcggcg agttgtgtct cagcccggtc
     2761 gggctttcgg cgaccaccaa gctggcgccg cggcgctacg gcgggcgcat gatgggcacc
     2821 tggtttctgt ttctagcgct cggcgaattg ctcgccggcc tgatcgccgg gcgcttcagc
     2881 gcgaaggacg tgcaggacat gccgcacatc tatctgacga ttgccgccgt cggaatcggc
     2941 gtcggcatcg tgctcgcgat cttctcgcgg ctcatccagc ggcgcctgat cgagaagtaa
     3001 ggcgagaaca gtgaggtacg agaggcggag gggggcgtct atcccgtcct tcttctcttc
     3061 ccgcttttct cattttacgc acgcgcaagc gcgtagacat ccacacgagt ggccccggcg
     3121 cgcttgagca cttgcgccgc ggcctccgcg gtgctgcccg ttgtaatcac gtcgtcgacg
     3181 atcgcgacat gaacgcccgc gagtgcgcgt cggcatgcga aggctccgcg cagattgcgg
     3241 cgacgttccc gccgcgggag cccgagttgc ggaggcgtcg gacgtgcgcg gctcagaagc
     3301 gcgggctccg cggcaacgtc gagcgactgc gccaggatcc gcgcgatttc cgccgcctga
     3361 ttgaaaccgc gtccggcgag gcgaccgcgg tgaaggggca cgggaacgag cacatccgga
     3421 cggggcaatt gatgcagatc aaggtattcg gcgagcgcga gcgcgagaat gcgaccgacc
     3481 ggaaggcggc cccggaactt gaaatcctgc agcaggcgat tgacgggcca agcatagacg
     3541 aaggccgccg tcgccgcgtc gtacagcggg gcgcgacggg cgcaggcgcc gcagcgagta
     3601 cccgggagat cgggcagagg cgtcgcgcag ttttcgcagg gatgatcatt ccacggcaag
     3661 tcgcgaaagc aggacgggca gagtgcgcct gcgccccgcg cgttgcaaca gaggcaacgg
     3721 gcggcgagta tgccggcgcc cgtcttccgt gcggcgcgaa gcgcagcggt gatgcgtgcc
     3781 cggcgcatcc ggggttccgg ggcgcggggc gcatcgggta tcatgatcgg acccacgatc
     3841 gggaactcaa ggtgtccggc ggcgagccca tcatcacccg catt
//
LOCUS       DASUJG010000396         3284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1337890, whole genome shotgun sequence.
ACCESSION   DASUJG010000396 DASUJG010000000
VERSION     DASUJG010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337890"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(252..707)
                     /gene="dut"
                     /locus_tag="VFX38_08445"
     CDS             complement(252..707)
                     /gene="dut"
                     /locus_tag="VFX38_08445"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006320136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX5314915.1"
                     /translation="MNRVEVKILRPALVERWGLPRYATAGSAGLDLRAALDTPLRLAA
                     GAAELIPSGIAIHIGDAGLAAMILPRSGLGHRHGIVLGNLLGLIDSDYQGELLISCWN
                     RGEHEYTIEPGERIAQLLFVPVAHPELAVVEEFTPSERGAGGFGHSGRH"
     gene            complement(736..1923)
                     /gene="coaBC"
                     /locus_tag="VFX38_08450"
     CDS             complement(736..1923)
                     /gene="coaBC"
                     /locus_tag="VFX38_08450"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX5314916.1"
                     /translation="MKGLEGRHIVLGVAGGIAAYKAPDLVRRLRERGAEVEVVMTAAA
                     AHFVTAATFQAVAHRPVHCDLWHPTAATAMDHLALAGWADLLLIAPATADLIARLANG
                     IADDLLTTLALATAAPLVLAPAMNSRMWRHPATRANVATLAARGARTLGPDDGPLAEG
                     GSGPGRMMAPEAIAVALAGGGALAGKRVLVTAGPTEEPIDPVRFVGNRSSGKMGYAVA
                     EATIAAGAEVTLVSGPTALAPPPGCEFVTVRTAAEMAAAVRNHVGDCELFIAVAAVAD
                     YAPAKSAGEKIKKTGETLRLELVRTPDILAEVAAGKSRPFCVGFAAETENLIANARAK
                     LTEKHLDLVCVNRVGDDAAFGRDDNALVAISTQGETDLGSGPKRLLAERLIALVAELL
                     SGR"
     gene            1983..2657
                     /gene="radC"
                     /locus_tag="VFX38_08455"
     CDS             1983..2657
                     /gene="radC"
                     /locus_tag="VFX38_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEX5314917.1"
                     /translation="MSIRDWPASERPREKLLAEGAARLSNPELLAIFLRTGVRGKSAL
                     DLAQDLIAAHGGLRPLLAAGEEEFTSTPGLGQAKYVQLQAALELARRHLLAQLEDRDS
                     ITSPAATRAFLTARLRDLPHEVFGCLYLDTRHRVLGFEELFRGTIDGASVHPREVVKA
                     ALAKNAAAVIFAHNHPSGVAEPSDADRRLTRRLADALALVDVRVLDHFVIGEGDPVSF
                     AERGLL"
     gene            2740..>3284
                     /locus_tag="VFX38_08460"
     CDS             2740..>3284
                     /locus_tag="VFX38_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314918.1"
                     /translation="MLRSVLLRVRVFRLCSAVVWPLALLFVYPLSLLKKKNPGGLFFL
                     FDRYCIGGAQGVFLDILAAVEDSPKQLYFTRRPADSGLKHLFYAVPNTEIREIDFWTC
                     NPLLRFFAVHFYAFYINRHRGARVLGSNSTFFYDLVPFLSRKVRSIDLFHNFQNYGNH
                     GFQFYGLATCRHLDVRVVVDR"
BASE COUNT          527 a         1079 c         1096 g          582 t
ORIGIN      
        1 ctcggcgctg atgctcgccg ccgcgcgcct gcgccacgtc gctggcaccg cctggggcgt
       61 caaacgctac ttgaacatgg agccgctgct caaacagcag cagctcagtg aacgggcttc
      121 ggcttgagtg aaactcatgg atcggatcac cgcccaatcg aaagtgcgaa agaatcttga
      181 cactaccctc cgaacgcagg ctttccccgt acacgtcacg aggaaacggg cgctgcatta
      241 agctggctgg gttagtggcg cccggaatgg ccgaagccgc cggcgccgcg ctcgctcggc
      301 gtgaattcct cgacgacggc gagttcgggg tgggcgacgg gcacgaagag gagttgcgcg
      361 atgcgctcgc ccggttcgat ggtgtattcg tgctcgccgc ggttccagca ggagatcaag
      421 agttcgccct gatagtcgga atcgatcagg ccgaggagat tgccgagcac gatgccgtgg
      481 cgatggccga gcccggagcg cggcagaatc atcgcggcga ggccggcatc gccgatgtga
      541 atcgcgattc cgctcgggat cagctccgcc gcaccggccg cgagtcgcag cggtgtatcg
      601 agtgcggcgc gcagatcgag ccccgccgag cccgcggtcg cgtagcgcgg aagaccccag
      661 cgctcgacaa gcgccggacg cagaatcttg acctcgactc gattcattcg ctgacgatcg
      721 cgagcacgct cgttcctacc ggccgctcag aagttcggcg acgagcgcga tcaggcgctc
      781 ggcgagcaat cgtttcgggc cgctgccgag atcggtttcg ccttgcgtcg aaatcgccac
      841 gagtgcattg tcgtcgcgcc cgaaggcggc atcgtccccg acgcggttga cgcagacgag
      901 atcgagatgc ttctcggtca gcttcgcgcg cgcgttcgcg atcaggtttt cggtttcggc
      961 ggcaaagccg acgcaaaacg gccgcgactt gccggccgcg acttcggcga ggatgtcggg
     1021 cgtgcgtacc agctccaggc gcagggtttc gccggtcttc ttgattttct cgcctgcgga
     1081 tttcgcgggc gcgtagtcgg cgacggcggc gaccgcaatg aaaagctcgc aatcgccgac
     1141 atggttccgc accgccgcag ccatctccgc ggccgtgcgt accgtgacga actcgcagcc
     1201 gggaggcggc gcgagtgcgg ttgggccgct taccagcgtc acttcggctc cggccgcgat
     1261 cgtagcctct gcgacggcgt agcccatctt gccggagctg cgattgccga cgaagcgcac
     1321 cggatcgata ggctcctcgg tcggaccggc ggtcacgagc acgcgcttcc ccgcgagcgc
     1381 accgccgccc gcaagcgcca cggcaatcgc ctcgggtgcc atcatgcgtc ccgggcccga
     1441 accgccttcg gcgagaggac catcgtcggg cccgagagtg cgcgcgccgc gcgccgcgag
     1501 cgtcgcgacg ttggcgcggg tcgcgggatg ccgccacatg cgcgaattca tggccggggc
     1561 gagaacgagc ggcgctgcgg ttgcgagcgc gagggtggtc aggagatcgt cggcgatgcc
     1621 gttggcgagg cgagcgatca ggtcggcggt tgcgggcgcg atcagcagca gatcggccca
     1681 gccggcgagc gcgaggtggt ccatggccgt cgccgccgtc gggtgccaga gatcgcagtg
     1741 aacgggccgg tgcgcgacgg cctgaaaggt cgccgccgtg acgaaatgcg cggccgccgc
     1801 tgtcatcacg acctccacct cggcgccgcg ctcgcgcaga cgccgcacga ggtcgggcgc
     1861 cttgtaggcg gcgattcctc ccgcgacgcc gagcacgatg tgcctgcctt cgagcccctt
     1921 catccatcgc ctccgtgaac ggcgctatat tagcccgcgg ggaacgacag ggaggggagg
     1981 tcatgagcat ccgcgactgg ccggcgagcg aacgcccgcg cgagaagctg ctcgccgagg
     2041 gcgccgcgag gctttcgaat ccggaacttc tcgccatatt tctgcgcacc ggcgtgcgcg
     2101 ggaaaagcgc gctcgatctt gcgcaggact tgatcgcggc gcatgggggg ctccgtccgc
     2161 tgctcgcggc gggcgaggag gaattcacga gcacgcccgg gctcgggcag gcgaaatacg
     2221 tccagttgca ggcggcgctc gaactggcac gccgacacct tctcgcgcaa ctggaggatc
     2281 gtgactccat taccagcccg gcggcgaccc gcgcctttct caccgcccgc ctgcgcgatc
     2341 ttccgcatga agtttttggc tgcctctatc tcgacacgcg ccaccgggtt ctcggctttg
     2401 aggaactgtt ccgcggcacg atcgacgggg caagcgtgca tccgcgcgaa gtcgtgaagg
     2461 ccgcgctcgc gaagaacgcc gcggccgtga tcttcgcgca caatcatccc tcgggggtcg
     2521 ccgagcccag cgatgccgat cgcagactca cgcgcaggct cgccgatgcg ctcgcgctcg
     2581 tggacgtgcg cgtgctcgat catttcgtca tcggcgaagg cgatccggtt tccttcgccg
     2641 agcgcggctt gctgtagcac ggcagcgcgg ccgtgcccga gccggggcgg cctgtatgat
     2701 agagcgcagt tgccagggcc gcgcgcccgg ggatatgtcg tgttgcggag cgttctcctc
     2761 cgggtgcgtg tgtttcgcct ctgctctgcg gtcgtctggc cgctcgcgct cctgttcgta
     2821 tatccgctca gcctgctgaa gaaaaagaat ccgggtggct tgttcttcct tttcgaccgt
     2881 tactgcatcg gcggcgccca gggcgtgttt ctcgacattc tcgcggccgt ggaagactcg
     2941 cccaaacaac tgtatttcac gcgcagaccc gccgactccg gcctcaagca tctcttttat
     3001 gcggtcccca ataccgagat ccgcgagatc gatttctgga cttgcaatcc gctgctgcgg
     3061 ttcttcgccg tgcacttcta cgccttttac atcaaccggc acaggggggc gagggtactc
     3121 ggatccaaca gtacattctt ctacgacctg gtgccgtttc tgagccgaaa agtgcgctcg
     3181 atcgatctgt ttcacaactt tcagaactac ggcaatcacg gatttcagtt ttatggcctt
     3241 gcgacctgcc gccatctgga cgtgagggtc gtggtggatc gctt
//
LOCUS       DASUJG010000397         2992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1583017, whole genome shotgun sequence.
ACCESSION   DASUJG010000397 DASUJG010000000
VERSION     DASUJG010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1583017"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /locus_tag="VFX38_08465"
     CDS             complement(<1..955)
                     /locus_tag="VFX38_08465"
                     /inference="COORDINATES: protein motif:HMM:NF016252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HEX5314919.1"
                     /translation="MTAMASEDHCVGGWFGRLGGLPALAGVVALGVTLLPLEAHAFGF
                     DDVVAQAKALVAKPWQPVAAIPAWLQYPTLDYDQYQQIRFRPGKRLWRGDGSRFNVMF
                     LPAGLYYTEPVTIHVVSGGVSRPFAFDKSLFTYPDKSFAQKIPSDLGYGGLVLTYPLE
                     PNAAYNSFLVFGGASYFRAVATGENFGLSARGLAVDTGLATPESFPFFRSFWLVKPAP
                     GADTMDVYALLDGEHASGAYRFTVHPGAPTVLDVTAVLFFRDHPARVGIAPLTSMFYY
                     GSNTTRPPGEWRRAVHDSSGLGVENGDGEWLWRPLINPDTVT"
     gene            complement(970..2835)
                     /gene="sppA"
                     /locus_tag="VFX38_08470"
     CDS             complement(970..2835)
                     /gene="sppA"
                     /locus_tag="VFX38_08470"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEX5314920.1"
                     /translation="MASSRGGFRRFVGGTLKGIDIARRVVVDILFIAIIVFILVLIFR
                     PKGPSVPASAALFLQPQGMLVEQTPDPVRRAERQLVGQAPTPVARVRDLVWAADTAAG
                     DSHIKAIVLDLDGFAGGSLAQLERVGAALRNFEKSGKPVIAYASDYSQGSYYLAAMAT
                     HAYLTTDQGIVALFGLAAYQNYYKDLIDKLKVQWHVFRVGKYKSYVEPFTRNDMSQPA
                     RQETTDLLDVLWNSYVGEVAHARGHSADEWTSLANHIPDALATTHGDAATLAVRSGLI
                     DGIVALPVLRAKIAKIVGGENAGGEYRHIDLSAYLGARAPGPAIANRAWDQVAIIVAE
                     GDIVTGSAPQGTIGADTMDDLLNQARNDDRIKAVVLDVNSPGGSALASDQILHAELAL
                     KASGKPLVVSMAGVAASGGYWISMAGDKIFASPATITGSIGIFGMLPTFEDTMAWAGV
                     HRDGVETAPLADFGDPFRALDPQAAKVFQLTINHGYAEFTGNVAKYRRLPLSHVDEIA
                     QGRVWPGTDAKRIGLIDDFGDLGAAVRSAASLAKLDNYGVIYLEPQLSPGEQFLVKLA
                     GDGSVRAVSRHLFGGGSDLVASLRPVWEPLARALALHDPNGVYAYCFCAAATKLR"
BASE COUNT          564 a          978 c          991 g          459 t
ORIGIN      
        1 tggtcaccgt gtccggattg atgagcgggc gccacaacca ctcgccatcg ccgttttcga
       61 cgccgagccc gctcgaatcg tgcacggcgc ggcgccattc ccccggcggg cgtgtggtgt
      121 tggaaccgta gtagaacatg ctcgtgagcg gggcgatccc gactcgcgcg ggatgatcgc
      181 gaaagaagag cacggcggta acgtcgagca cggtcggcgc gcccggatgc accgtgaagc
      241 ggtaggcgcc gctcgcatgt tcgccgtcga gaagcgcata gacgtccatg gtgtcggcgc
      301 cgggtgccgg tttcaccagc cagaaagaac gaaagaacgg aaaggactcg ggcgtggcga
      361 gaccggtatc gacggcgagc ccgcgcgccg acagcccgaa gttctcgccg gtcgcgacgg
      421 cgcggaaata actcgcgccg ccgaagacca gaaaggaatt gtaggccgcg tttggctcga
      481 gcggataggt gagaacgagg ccgccgtagc cgagatccga cgggattttc tgcgcaaacg
      541 acttgtcggg ataggtaaaa agcgacttgt cgaaggcgaa ggggcgcgat acgccgccgc
      601 tgacgacgtg aatcgtcacg ggctcggtgt aataaaggcc cgccggcagg aacattacgt
      661 tgaagcgact tccgtccccc cgccacaggc gcttgccggg acggaaacga atttgctggt
      721 actgatcgta atcgagcgtc gggtactgca accaggcggg aatcgcggcg accggctgcc
      781 agggcttcgc gacgagcgcc ttcgcctgcg ccacgacgtc atcgaagccg aaggcatgcg
      841 cctcaagcgg caacagcgtg acgccgagcg cgacaacgcc agcgagggcc ggcaatccgc
      901 cgagcctgcc aaaccagccg ccgacgcaat gatcctcgct cgccatggcc gtcatcgctt
      961 ggcgttcact cagcgcagct tcgtcgcagc ggcacagaag cagtaggcat aaacgccgtt
     1021 cggatcgtgc agcgccaggg cccgcgcgag cggctcccac acgggacgca gcgacgcaac
     1081 cagatcgctc cccccgccga acagatgccg ggacaccgcc cgcacgctgc cgtcgcccgc
     1141 gagcttcacg aggaactgct cgcccggaga aagctgcggc tcgagataga taaccccgta
     1201 gttgtccagt ttcgcgagcg aggccgccga acgtaccgcg gcaccgagat cgccgaagtc
     1261 gtcgatgaga ccgatgcgct tggcatccgt ccccggccac acgcggccct gggcgatttc
     1321 gtccacatgc gagagcggca ggcggcggta cttggcgaca ttaccggtga actcggcgta
     1381 accgtggttg attgtgagct gaaagacctt ggcggcctgc ggatcgagcg cccggaaggg
     1441 atcgccgaaa tcggcgagcg gcgccgtctc gaccccgtcg cgatgaacgc ccgcccaggc
     1501 catcgtgtcc tcgaaggtcg gaagcatgcc gaagatgcca atcgaacccg tgatcgtagc
     1561 gggagacgcg aaaatcttgt cgcccgccat cgaaatccaa taaccgccgg aagcggcgac
     1621 gcccgccatc gaaaccacga ggggtttccc actcgccttg agcgcgagtt ccgcgtggag
     1681 aatctgatcc gaggcaagcg cgctgccccc ggggctgttc acgtcgagca cgaccgcctt
     1741 gatgcgatcg tcgttcctgg cctgattcag aagatcgtcc atcgtgtcgg cgccgatggt
     1801 cccctgcggc gccgagcccg tgacgatgtc gccctcggcg acgatgatcg cgacctgatc
     1861 ccaggcgcga ttggcgatag cggggccggg cgcgcgcgcg ccgaggtagg cgctcagatc
     1921 gatgtgacga tactcgccgc ccgcgttttc tcctcccacg attttcgcga tttttgcccg
     1981 cagcacggga agcgccacga tcccgtcgat gaggccactg cgcaccgcga gcgtcgccgc
     2041 gtcgccatgc gtcgtcgcga gcgcatcggg aatatggttc gcgaggctcg tccactcatc
     2101 ggcgctgtgg ccgcgagcgt gggcgacttc accgacgtag ctgttccaca gtacatcgag
     2161 cagatccgtg gtttcttgcc gcgccggctg cgacatgtcg ttgcgggtga agggttcgac
     2221 gtaggacttg tacttgccga cccgaaagac gtgccactgg accttgagct tgtcgatcag
     2281 gtccttgtag taattctgat acgcggcgag cccgaaaagc gcgacgatgc cctgatcggt
     2341 cgtgagatag gcgtgcgtcg ccatcgccgc gagatagtac gacccttgcg agtagtcgct
     2401 cgcgtaggcg atcaccggct tgccgctctt ctcgaaattg cgcagcgccg cgcccacccg
     2461 ctcgagctgc gcgagactgc cgccggcgaa gccatcgaga tcgagcacga tcgccttgat
     2521 gtgcgaatcg cccgctgcgg tatcggcggc ccagacaaga tcgcgcaccc gggcaaccgg
     2581 cgtcggagcc tggcccacga gctgccgctc cgcgcggcgc accggatcgg gggtctgctc
     2641 gaccagcatg ccctggggtt gcaggaagag cgccgccgag gcggggaccg acgggccctt
     2701 gggacggaag atcagcacca gaatgaaaac gatgatcgcg ataaacagga tgtcgacgac
     2761 gacgcgccgg gcgatgtcga tgcccttgag cgtgccaccc acgaagcgcc ggaagccgcc
     2821 gcgagaacta gccatccgcc tcatccgcga acccgtgatc ggagcagtgt agtacgcgcg
     2881 ggcttcgcgg cgcaaagcgg ccggcgcccc cgcggcgcgg gcttcgcgtc aaattgcggg
     2941 agaatcgccc cggctctgcg cttgcagacg aacccgttcc cgccggaacc cg
//
LOCUS       DASUJG010000398         2676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_483189, whole genome shotgun sequence.
ACCESSION   DASUJG010000398 DASUJG010000000
VERSION     DASUJG010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483189"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..924
                     /locus_tag="VFX38_08475"
     CDS             61..924
                     /locus_tag="VFX38_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX5314921.1"
                     /translation="MADWAASSGFESLEIACWPRTTGATRRYAGTSHIDVANLSDGQA
                     QEIVGEIAEKGLAISGLGYYPNPMHPDAEVRAQAIGHIKLVISAAARMGVPFMNTFMG
                     GDASKTQDENWETALQVWPEIVNHARENGVAITIENCPMIFSRDEWPAGHNIAWSPYI
                     WRRIIAEWGGVIGLNYDPSHLVWLMIDQERFIREFGPHILHVQAKDVQIDHDGLYERG
                     TLSGGIGWQIPRLPGLGDADWPRIFSALWRAGYDGDIIIEHEDHDFEATDELVKRGFL
                     LARDVLRPYIK"
     gene            921..1673
                     /gene="deoC"
                     /locus_tag="VFX38_08480"
     CDS             921..1673
                     /gene="deoC"
                     /locus_tag="VFX38_08480"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEX5314922.1"
                     /translation="MTSTPARSIDHLTERDIAKTIDHSLLRPELDDTQIAEGCRLAAE
                     YDVASVCVRPADVARARELLAGTDVAVDATVGFPHGNHLTATKVFETQRALDDGATEI
                     DMVLQIGALKSGRDADVQADIAAVVEVAHARGAIVKVIFENAYLTDDEKVRACRLSEA
                     AGADFVKTSTGYAPSGATHDDLRLMRANTSPHIAIKAAGGVRTLDALLDVMALGVTRI
                     GATATRTIIDDFRARKAGRAPVASTASGEGGY"
     gene            1796..>2676
                     /locus_tag="VFX38_08485"
     CDS             1796..>2676
                     /locus_tag="VFX38_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019859384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX5314923.1"
                     /translation="MTTMTRMKTVGAMLAVAILTAACSGGTTPSATGGASQSPAGSGS
                     QPAGGSYTIGYSNGGGVGNGFREEQVCTAKAQALASGEVSNLTTIHRNTDAAGQLSDI
                     RDLIAKGVNAIVFNPNDPEALNPALEEAHAAGIVTVSVDAYVTDPNTYNLYNNQIKYA
                     ELGAKWLFDQLGGKGTVYYMRGLAGNSADSDRDVGFKNILKDYPDIKIVPNADGVFTG
                     WDPATTTQLINDFLASGQYDSIDGIWTSGMDSQVVDAIKAANKPYVPIVGADLGAFVG
                     QLLNPTDYPGLEGAAVT"
BASE COUNT          501 a          908 c          870 g          397 t
ORIGIN      
        1 cgccacgtcg gcgtagacgg cgcggaacat cgcgccgaac gtgtcgccga agccctcgac
       61 gtggccgact gggccgcgtc gagcggcttc gagagcctgg agattgcctg ctggccgcgg
      121 acgacggggg caacccggcg ctacgccggc acgtcgcaca tcgacgtcgc caacctgtcc
      181 gatggccagg cacaggagat cgtcggcgag atcgccgaaa agggcctggc catctcgggt
      241 ctgggctact acccgaaccc gatgcacccg gatgcggagg tgcgggcaca ggccatcggc
      301 catatcaagc tggtcatcag cgccgcggcg cgcatgggcg tgccgttcat gaacacgttc
      361 atgggcggcg atgcatcgaa gacccaggac gagaactggg agaccgccct gcaggtctgg
      421 cccgagatcg tgaaccatgc gcgcgagaac ggtgtagcta tcacgatcga gaactgtccc
      481 atgatcttca gccgcgacga gtggccggcc gggcacaaca tcgcctggtc accgtacatc
      541 tggcggcgga tcatcgccga gtggggtggc gtgatcggcc tcaactacga cccgtcgcat
      601 ctcgtctggc tcatgatcga ccaggagcgt ttcatccgtg aattcgggcc gcacatcctg
      661 cacgtgcagg ccaaggacgt ccagatcgac cacgacggcc tctacgagcg cggaacgctg
      721 tcggggggca tcgggtggca gattccgcga ctgccggggc tgggtgacgc cgactggccc
      781 cgcatcttct cggcgctgtg gcgtgcggga tacgacggcg acatcatcat cgagcacgag
      841 gatcatgact tcgaggcgac cgacgagctg gtgaagcgag gcttcctgct ggctcgcgac
      901 gtcctgcgtc cgtacatcaa atgacaagca cacccgcacg ttccatcgac cacctcaccg
      961 agcgcgacat tgccaagacg attgaccact cgctgttgcg ccccgagctg gacgacaccc
     1021 agatcgccga aggctgccgg ctggcggcgg agtacgacgt cgcatcggtc tgcgtccggc
     1081 ccgccgacgt cgcgcgagcg cgcgagctgc tggccggaac cgatgtcgcc gtcgacgcca
     1141 cggtcggctt tccgcacggc aaccatctga ccgcaaccaa ggtcttcgag acccagcgcg
     1201 ccctggacga cggggccacc gagatcgaca tggtgctgca gatcggcgcc ctgaagtccg
     1261 gccgcgacgc ggacgtgcag gccgatatcg ccgccgtggt cgaggtggcg cacgcgcgcg
     1321 gcgcgatcgt gaaggtcatc ttcgagaacg cctacctgac cgatgacgag aaggttcgcg
     1381 cgtgccgcct gtcggaggcc gcgggcgccg atttcgtcaa gacctcgacc ggctatgccc
     1441 ccagcggtgc gacgcacgac gatctgcgcc tgatgcgcgc caacacctcg ccgcatatcg
     1501 caatcaaggc tgcggggggc gtgcgcacgc tcgatgcgct gctcgacgtc atggcgctgg
     1561 gcgtcacgcg catcggcgcg accgccacca ggacgatcat cgatgacttc cgcgcccgca
     1621 aggccggacg cgctccggtc gccagcaccg cgagcggcga gggtggttat tgaacctgtt
     1681 cgatttcgcc taccgcgaac acgcgcggcg gatggcgctg gccatcgaca agcaaggagg
     1741 tgaatgacac actgccgcgt caacggggaa tccatcagtt gaggaattgg agcggatgac
     1801 gacgatgaca cgtatgaaaa cggtcggggc gatgctcgcg gtggcgatcc tgactgccgc
     1861 gtgcagcgga ggcacgacgc cttccgcgac cggcggcgct tcgcagtcgc ctgccggatc
     1921 cggcagccag cccgccggcg gcagctacac cattggctac tccaatggtg gcggcgtcgg
     1981 caacggcttc cgggaggagc aggtctgcac cgccaaggcg caggcactcg cctcgggcga
     2041 ggtctcgaac ctcaccacca tccaccgcaa cacggacgcc gcgggccaac tctccgacat
     2101 tcgcgacctg atcgccaagg gcgtcaacgc gatcgtcttc aacccgaacg accccgaagc
     2161 gctcaatccg gcgctcgaag aggcgcacgc ggccggcatc gtgacggtct cggttgacgc
     2221 gtatgtcacc gacccgaaca cctacaacct ctacaacaac cagatcaagt acgcggagct
     2281 cggcgccaag tggctgttcg accagctcgg cggcaaggga accgtttact acatgcgcgg
     2341 cctcgccggc aactcggccg acagcgaccg cgacgtcggt ttcaagaaca tcctcaagga
     2401 ctacccggac atcaagatcg tcccgaacgc ggacggcgtc ttcaccggtt gggatccggc
     2461 taccaccacg cagctgatca atgacttcct ggccagcggc cagtacgact cgatcgacgg
     2521 catctggacc tcgggcatgg actcgcaggt cgtggacgcg atcaaggccg ccaacaagcc
     2581 ctatgtgccg atcgtcggcg ccgacctggg cgcgttcgtc ggccagctgt tgaacccgac
     2641 cgactatccg ggcctcgagg gcgcggccgt caccaa
//
LOCUS       DASUJG010000399         4417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_848340, whole genome shotgun sequence.
ACCESSION   DASUJG010000399 DASUJG010000000
VERSION     DASUJG010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848340"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1739)
                     /locus_tag="VFX38_08490"
     CDS             complement(<1..1739)
                     /locus_tag="VFX38_08490"
                     /inference="COORDINATES: protein motif:HMM:NF016252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HEX5314924.1"
                     /translation="MTKWTGIVAAAAALALAACATQQSVETAQDRAEQALAASRNFDA
                     KLQAALDEQARREQTIMARLADIQTRQDAASAKQSSLSQQLSEVHGELREQRKTIAKV
                     TPVRSAPVPGTPAPAPASLAAPVSSSAADAMYATVTARARQIAAGTTPAPSLAPVPAV
                     LQHLDYGTYEAIGYDGKLADWSGSDSFKLSFHPAGFVFDQSIEVHLLGQKADAALGFT
                     VDDFHWPGALRDQAPHKVPIAGFRAGQLYDGGLSAPFLDFLGASYFQATPVGKPFGLY
                     ARAVAIDTGVSNTAEEFPRFSEFWIGAASGSQLDVVALLTSASIDGAYHFKVLPNVKE
                     TAIEVNAALFLTRTVSQLGLAPLTSMYLQGPDSLNRPDTMAKAVHDSDGLLIHSGQGW
                     SWIPLFNPSRLQVVQIPVTDLRGFGLLQRDRKYDDYQSPQDAYQKHPSAWVEPTNDWG
                     RGVIKLVEIPAQYPSNNNIVAFWTPANPPAPKQLFRLAYRLDWSIGGPAEGELGRVLA
                     TRIVDLKSGHYAVMIEFGGGSLANLPGWVKVQPTVDTNGKDLKIDKVSLNKIAETGRW
                     RLSFEVSGGGGDE"
     gene            complement(1743..3863)
                     /gene="mdoH"
                     /locus_tag="VFX38_08495"
     CDS             complement(1743..3863)
                     /gene="mdoH"
                     /locus_tag="VFX38_08495"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF003958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HEX5314925.1"
                     /translation="MRELPPAAPALCRTPLAPSPKPSYRPRRQHWERVLLWRRSILFA
                     LVVAPSAFMGWLFSSALPPPHNDPLDLALAAAFGLLFAWVAIWFWTAMAGFFLLLGKR
                     RMKLPGAQASESSGTVARVAVVIPVYQEEPALVAGGIAALYEGLAERNSLERYDFFIL
                     SDSADADQWIAEEATWLRLTRELDAAGRIFYRRRRSRSKRKPGNIADFCRRWGRNYAY
                     LIMLDADSVLAAETAEELVARMDANPSAGIIQTFPQVVNQHTLYGRLQQFAAALYGRM
                     FTAGLHFWQLGAGMYWGHNAIIRLDAFMRHCMLPSIHGGPLGGDILSHDFVEAALMRR
                     AGYDVWIAYDLEGSYEETPPRFADDVIRDRRWSRGNLQHLRLLGMHGLHGAHRAMFVA
                     GAFSYISSGIWLIFLALGTAIMIQHAWSAPNYFPQAHSPFPHWPVWHHGIQIALIATA
                     FGTLLLPKLFGWLLAILRRSSRRRFGGGIRLTIGMLIELLVSGLFAPARMLLHSFFVV
                     AALLGARVEWGGQQRGLISIRPQAVMRRHFIGFALGALWLALALQVSLTLFFWLLPAL
                     AGLFLTPLLEIVTGSAKLGRRLRNKRILVTGEELAPPAALLARREILRRFLAAAMPGF
                     AEVFRDAATHALHVGLLRRRRLPHGAVGYERLAAAARLLRYGPAALDAEERLRLLYDR
                     ELLKSLYRRLAEPRRGANETLQEI"
     gene            complement(3925..>4417)
                     /locus_tag="VFX38_08500"
     CDS             complement(3925..>4417)
                     /locus_tag="VFX38_08500"
                     /EC_number="4.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187818.1"
                     /note="catalyzes the formation of acetoacetate and
                     acetyl-CoA from 3-hydroxy-3-methylglutaryl-CoA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX5314926.1"
                     /translation="TAIGCPYEGAIAPDAVARVAKELDALGCYEISLGDTIGVGTPRK
                     AQRMLEAVAVGVPIERLAVHFHDTYGQALANILACLELGAAVVDSSVSGLGGCPYAKG
                     ATGNVATEDLLYMLIGMGIETGVDLDALLDAGDFINRALSRRAESRLGRVAREQSRRF
                     ATP"
BASE COUNT          781 a         1402 c         1512 g          722 t
ORIGIN      
        1 tcttcgtcgc cgccaccacc gctcacttcg aagctcaggc gccagcgtcc cgtctcggcg
       61 atcttgttca gcgagacctt gtcgatcttg agatccttgc cgttggtgtc gaccgtcggc
      121 tgcaccttta cccatccggg aagattcgcg agcgagcccc cgccgaattc gatcattacc
      181 gcgtaatgtc cgctcttgag atccacgatg cgggttgcga gcacgcgccc gagttccccc
      241 tccgccggtc cgccgatcga ccagtcaagg cgataggcga ggcggaagag ctgcttgggc
      301 gcgggaggat tcgcgggtgt ccagaaggcg acgatgttgt tgttgctcgg atactgcgcg
      361 ggaatttcca cgagcttgat gaccccgcgg ccccaatcgt tggtcggctc gacccaggcg
      421 ctcggatgct tctgataggc gtcctgcggg gactggtaat cgtcgtattt gcgatcacgc
      481 tggagcagcc cgaagccacg gagatccgtc accggtatct gcacgacctg caggcgtgag
      541 ggattgaaga gcggaatcca ggaccatccc tgaccgctgt ggatgagaag accgtcggaa
      601 tcgtggacgg ccttggccat cgtgtccggg cggttcaggc tgtcggggcc ttgcagatac
      661 atgctcgtga gcggggccag tccgagttgg ctcacggtgc gcgtgagaaa cagcgcggcg
      721 ttgacctcga tcgccgtctc cttcacgttc ggcagaacct tgaagtgata ggcgccgtcg
      781 atgctggcgc tggtgagaag cgccaccacg tcgagctgcg agccgctcgc ggcgccgatc
      841 cagaactcgc tgaagcgcgg aaactcctcg gcggtattgc tcacgcccgt gtcgattgcg
      901 actgcgcgcg cgtacaagcc gaacggtttt cccacgggcg tggcctgaaa gtagctcgcg
      961 ccgagaaaat cgagaaacgg cgcggaaagc ccgccgtcgt acagttgccc ggcgcggaat
     1021 cccgcaatcg gaactttgtg cggcgcctgg tcgcgcagcg ccccgggcca gtgaaaatca
     1081 tcgacggtga agccgagggc agcgtcggcc ttctgcccga gcagatgcac ctcgatggat
     1141 tgatcgaaca cgaagccggc ggggtgaaag ctcagcttga agctgtcgct tcccgaccag
     1201 tcggcgagtt tgccatcgta gccgatggcc tcgtaggttc cgtagtcgag atgctgcagc
     1261 accgccggca ccggcgcgag cgagggtgcc ggagtcgttc cggccgcgat ttgtcgtgca
     1321 cgcgcggtga ccgtagcgta catggcgtcg gccgccgaac tgctcacggg cgcggcgagc
     1381 gaggcgggcg ccggcgccgg cgtgcccggc accggagcgc tgcggaccgg cgtcaccttg
     1441 gcgatggtct tgcgttgctc gcgaagctcg ccgtgaactt cggaaagctg ctgcgacaag
     1501 ctgctctgct tggcgctcgc tgcgtcctgc cgggtttgga tgtcggccag cctggccatg
     1561 atcgtctgct cgcggcgcgc ctgctcgtcg agcgccgcct gcaatttggc gtcgaagttg
     1621 cggctggccg cgagcgcctg ttcggcccga tcctgcgccg tctccacgga ttgttgcgtc
     1681 gcgcaggcgg cgagagcgag agcggctgcc gccgcgacga ttccggtcca cttcgtcatg
     1741 aactaaatct cttggagcgt ttcgttcgcg ccgcgccggg gttcggcaag acgccggtac
     1801 agcgatttca gcaactcccg atcatacagc aggcgcaggc gctcctcggc atcgagtgcc
     1861 gccggcccgt agcgcaggag tctcgcggcg gcagcgagcc gttcgtagcc gacggcgcca
     1921 tgcggcaggc gacgccggcg aagcaggccg acatgcagcg cgtgggtcgc ggcgtcgcgg
     1981 aacacctcgg cgaatcccgg catcgcggcg gcgagaaatc ggcgcaggat ctcgcggcga
     2041 gcgagcaggg cggccggcgg agcgagttcc tccccggtga cgaggatcct cttgtttcga
     2101 agccttcgcc cgagcttcgc actcccggtc acgatctcca gcagcggggt gagaaaaagc
     2161 cccgcgagcg ccggcaacag ccagaaaaag agggtcaggc tcacctgcaa cgcgagtgcc
     2221 agccacaagg cgccgagggc gaaaccgata aagtgccggc gcatgacggc ctgcggacgg
     2281 atggagatca ggccgcgttg ctggccaccc cattccacgc gcgccccgag cagggccgcg
     2341 acgacgaaga agctgtgcag cagcatgcgg gccggagcga acagccccga caccagcagt
     2401 tcgatcagca tgccgatcgt gaggcggatg ccgccgccga agcgcctgcg gctcgagcgg
     2461 cgcaggattg cgagcagcca gccgaagagc ttcggcagga gcagcgtgcc gaaggccgtc
     2521 gcgatgagag cgatctgaat cccgtgatgc cacacgggcc agtgcggaaa cggcgagtgg
     2581 gcctgcggga aatagttggg cgcgctccag gcatgctgga tcatgatcgc ggtgccgagc
     2641 gcgagaaaga tcagccagat gccggaggag atgtagctga aggcgccggc gacgaacatc
     2701 gcgcggtgcg ctccgtgcag gccgtgcatc ccgaggaggc gcagatgttg caggttgccg
     2761 cgcgaccagc gccggtcgcg aatgacatcg tcggcgaagc gcggcggtgt ttcctcgtag
     2821 ctgccctcga gatcgtaggc gatccacacg tcgtagccgg cgcggcgcat cagcgccgct
     2881 tccacgaagt cgtggctgag aatatcgccg ccgagcggac cgccgtgaat gctcggaagc
     2941 atgcagtggc gcatgaaggc gtcgaggcgg atgatcgcat tgtgccccca gtacatgccg
     3001 gcgccgagtt gccagaagtg caatcccgcg gtaaacatcc gcccgtagag tgccgcggcg
     3061 aattgttgca gccttccgta gagcgtgtgc tgattcacga cctgcggaaa ggtctggatg
     3121 atgccggcac tcgggttggc atccatgcgc gcgaccagtt cctcggccgt ttcggcggcg
     3181 agaacactat cggcgtcgag catgatgagg taggcgtaat tgcggcccca gcgccggcag
     3241 aagtccgcga tattccccgg tttgcgcttg ctgcggctgc gacggcggcg ataaaagatc
     3301 cgcccggcgg cgtcgagttc gcgggtaagc cgcaaccatg tcgcttcctc cgcgatccat
     3361 tgatcggcgt cggcgctgtc gctcagaatg aaaaagtcgt agcgctcaag ggaattcctc
     3421 tcggcgagcc cctcatacaa cgccgcgatt ccgccggcga ccagggccgg ctcttcctga
     3481 tataccggaa tcaccaccgc gacgcgcgcg acggtgccgg aggattccga ggcctgcgct
     3541 cccgggagtt tcatgcgccg tttgccgagg aggagaaaga atccggccat cgccgtccag
     3601 aaccagatcg cgacccaggc gaagagcagg ccgaaggcgg ccgcgagagc gagatcgagc
     3661 ggatcgttgt gcggcggcgg cagcgcgctc gaaaacagcc agcccatgaa ggcgctcggc
     3721 gcgacgacca gcgcaaaaag tatcgagcgc cgccacagga gcacgcgttc ccagtgctgc
     3781 cggcgggggc gatacgaggg cttgggcgaa ggcgcgagcg gcgtgcggca aagcgccggc
     3841 gcagcaggcg gcaattcgcg catcccggtt gtggaattgc atgcgggcgc aatcgtcacg
     3901 gctgccgaga gcccgttcgg tggctcaagg cgtggcaaag cgtcgactct gctcgcgtgc
     3961 gactctcccg aggcggcttt ccgcacggcg cgaaagcgct cgattgatga agtctcccgc
     4021 atcgaggagc gcgtcgagat ctacgcccgt ttcgatcccc atcccgatca gcatgtaaag
     4081 caaatcttcc gtcgcgacgt tccccgtcgc gcccttcgca tagggacatc ccccgagtcc
     4141 ggaaaccgag gagtccacga ccgcggcgcc gagttcgagg caggcgagga tgttggcaag
     4201 cgcctgcccg taggtatcgt ggaaatgcac ggcgagtctt tcgatcggta ccccgaccgc
     4261 aaccgcctcg agcattcgct gcgcctttcg cggtgtgccg acgccgatgg tgtcgccgag
     4321 agaaatctcg taacagccga gcgcgtcgag ttccttcgcg acccgcgcca ccgcgtcggg
     4381 cgcgatcgcg ccttcgtaag ggcagccgat tgcggtc
//
LOCUS       DASUJG010000400         2637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_848342, whole genome shotgun sequence.
ACCESSION   DASUJG010000400 DASUJG010000000
VERSION     DASUJG010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848342"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1175
                     /locus_tag="VFX38_08505"
     CDS             <1..1175
                     /locus_tag="VFX38_08505"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5314927.1"
                     /translation="TPSMPAQNPTPPSPEDLDEPPTPAERRVRSGAVIGILVLMILYT
                     AYFAAPILIPMALAFLLSLLLSPAVGWLARLRIPRVIGAALVLALLLVGFGAAIYGLS
                     APATHWVKEAPEAIAKIEKMFHSVNGSFNELQKATQKVESLPATNPPASETPRPVKVV
                     NANPGLVGEIFATAPDILAGVGIAIVLLYFLLAASDSFLRSLAHVVPRWSDKKRVVEV
                     ARGIQRHISAYLLTITLINLTLGLVDGLILWALGMPNPILWGAMIAIFNYVPYVGAAT
                     AIVVLTLIALLNFDGLGHVIVVPAAILAVAILEGQFLTPRIVGRRLVLSPVAVFLALV
                     VCGWIWGIAGAIMAVPFLASFKIMCEEIEPLRPIAQFLTLYSIHRGRMPRDDAGVE"
     gene            complement(1193..1639)
                     /locus_tag="VFX38_08510"
     CDS             complement(1193..1639)
                     /locus_tag="VFX38_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314928.1"
                     /translation="MALVARLKDRLQGYTIALGAGVVVALLLATVAGLCLAALYLYLI
                     GIFIAPFAALLTAAGALFLAGLIALGTWIARTCCEDSGGSSRKSGARATSGDGFDSML
                     LELAAEWIRSNAKGASVAAGLAGLAAGVSPQLRKLLISLLASRGKN"
     gene            complement(1647..1907)
                     /locus_tag="VFX38_08515"
     CDS             complement(1647..1907)
                     /locus_tag="VFX38_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 C-terminal domain-containing protein"
                     /protein_id="HEX5314929.1"
                     /translation="MATTEQDIQTLKSDFGSLRRDVAELTSAIKNLVVDESRVGYEKI
                     KETTGKSVRQVEHEIEEHPFASVGIAMGVGFLIGVLLDRLTR"
     gene            2140..>2637
                     /locus_tag="VFX38_08520"
     CDS             2140..>2637
                     /locus_tag="VFX38_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314930.1"
                     /translation="MAMNEHRPSALAATEVAALRPTGGLACVLTDLALVRIAGRDATA
                     FLDAQCTAAIGADGDAVTATALADAKGRVLGTFLALADGDDEWLLAVTASEEEWLRGH
                     LGRFVFRSRVAIAAATDKRLIGVLGPDADRALAAAGLPAPPENRLVRAGPLLVCGIGS
                     GRRLVI"
BASE COUNT          414 a          889 c          841 g          493 t
ORIGIN      
        1 agacaccgag catgcccgcg cagaacccga cgcctccgtc cccggaagac cttgacgaac
       61 cgccgacgcc ggcggagcgg cgggtacgct ccggcgcggt catcggcatt ctcgtcctga
      121 tgatcctgta caccgcctat ttcgcggcgc cgatcctcat tccgatggcg ctcgccttcc
      181 tgctgagcct tctcctctcc ccggcggtcg gctggctggc gcgcctgcgc attccgcggg
      241 tcatcggcgc cgcactcgtt ctcgcgctcc tgctggtcgg cttcggtgcg gcgatctacg
      301 ggctttccgc accggcgacc cattgggtga aggaggcgcc ggaggcgatc gccaagatcg
      361 agaagatgtt tcactcggtc aacggatcgt tcaacgagct tcagaaggcg acgcagaaag
      421 tcgaaagtct ccccgccacc aatccgccgg cgagcgaaac tccgcgaccg gtgaaggtgg
      481 tgaacgcgaa tccgggcctt gtcggggaaa tattcgcgac cgcgcccgat attctcgccg
      541 gcgtaggcat cgcgatcgtg ctcctgtact tcctgctcgc cgcgagcgac agcttcctgc
      601 gcagccttgc gcacgtcgtg ccgcgctgga gcgacaagaa gcgcgtggtc gaggtcgcgc
      661 gcggaattca gcggcatatt tccgcctatc tgctcacgat cacgctgatc aatctcaccc
      721 tcgggctggt ggacgggctc atcctgtggg cgctcgggat gcccaatccg atactttggg
      781 gtgcaatgat cgcgattttc aattatgtac cctacgtcgg ggcggccacc gcgatcgtgg
      841 ttctgacgct gatcgcgctg ctcaacttcg acggcctcgg ccacgtcatc gtcgttcccg
      901 cggccattct cgcggtcgcg atcctcgagg gccagttcct cacgccgcga atcgtgggcc
      961 gccgcctcgt gctgagcccc gttgcggtgt tcctcgcgct cgtcgtctgc ggctggatct
     1021 ggggcattgc cggcgccatc atggcggtcc cgtttctcgc gagcttcaag atcatgtgcg
     1081 aggagatcga acccttgcgc ccgatcgccc aatttctcac gctctactcg attcaccgcg
     1141 ggcgcatgcc tcgcgacgac gcgggagtcg aatgaggccg gcgcgccggc gactagttct
     1201 tgccgcggct tgccagaagc gagatcagca gcttgcgcaa ttgcgggctc acgccggccg
     1261 cgagaccggc aagtccggcg gcgacgcttg ctcctttcgc attcgagcgt atccactccg
     1321 cggccagttc gagcagcatc gagtcgaacc cgtcgccgga tgtcgcgcga gcaccagatt
     1381 ttcgcgagga gccgccggaa tcctcgcagc aagtccttgc aatccaggtg ccgagcgcga
     1441 tgagacccgc gagaaacagc gcgccggcgg ccgtcagaag cgccgcgaag ggggcgatga
     1501 agatcccgat cagatacaga tacaaggccg cgaggcacag gcctgcgacg gtggcgagca
     1561 ggagcgcgac gaccacgccc gcgcccagcg cgatcgtgta gccctggagc ctgtccttca
     1621 gacgcgcgac gagcgccatc ggttttctac cgggtcagcc gatcgagcaa cacgccgatg
     1681 agaaacccga cgcccatcgc gatgccgacg ctcgcgaagg gatgttcctc gatctcgtgc
     1741 tcgacctggc gcacggattt gcccgtcgtt tccttgatct tctcataacc gacacgcgat
     1801 tcgtccacga cgagattctt gatcgcgctc gtgagttcgg caacgtcccg gcggagactg
     1861 ccgaagtcgg acttgagggt ttgaatatcc tgttctgtgg tagccatgta gttcgtcctc
     1921 atgctggaat tgcttgtttc gcgcaagctc cgtcacgagt ttgcgccggc agtcgccggt
     1981 cccgggggcc gacccgccgc tccgcagaat tcgagggaat cgtgcggaaa ttcttctacg
     2041 tcttcttgta agcttcgatt ctagcatcgc gcggcggggc atctcaaggg cgctgcgaag
     2101 tgccgcggcg gctttgcagc ctgcccgggg gactacgata tggcgatgaa cgagcatcgc
     2161 ccgagcgctc tcgccgcgac ggaagtcgca gcccttcgtc ccaccggcgg ccttgcctgc
     2221 gtgctgacgg acctcgcgct cgttcgtatc gccgggcgcg acgccaccgc ttttctcgac
     2281 gcccagtgca cggccgcgat cggcgcggat ggagatgccg tgacggcgac ggcgctggcg
     2341 gatgccaagg gccgcgtact gggcactttt ctggcgctcg ccgacggcga cgacgaatgg
     2401 ctgctcgcgg taaccgcgag cgaagaagaa tggcttcggg ggcatctcgg tcgcttcgtg
     2461 ttccgctccc gggtcgccat cgccgcggcg acagacaagc gcctcatcgg agtgctcggt
     2521 cccgatgccg atcgggcgct cgccgcggcg ggtttgccgg cgccgccgga aaaccgtctc
     2581 gttcgcgccg gacccctcct cgtctgcggc atcggcagcg gccgccggct ggtcatc
//
LOCUS       DASUJG010000401         5777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1216242, whole genome shotgun sequence.
ACCESSION   DASUJG010000401 DASUJG010000000
VERSION     DASUJG010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5777
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216242"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VFX38_08525"
     CDS             <1..768
                     /locus_tag="VFX38_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5314931.1"
                     /translation="PYAGYLGDGGDTLAYLETVLADRASGVDKPAAIIVETVQAEGGI
                     NVASAGWLRRLGAIARANDVLLIVDDIQVGCGRTGPFFSFEEAGIVPDMVTLSKALSG
                     YGLPLAVVLIRPELDQWEPGEHNGTFRGHNPAFVTAAAALDFWRDDALELATCEKAAA
                     LRAALENITDANDGIAEVRGRGLIQGLAFREPGLANLVSAEAFRRGLVIETAGAESEV
                     LKCLPPLTIGADELGEGIDIIADSVAAATTSRRRDAA"
     gene            803..1210
                     /locus_tag="VFX38_08530"
     CDS             803..1210
                     /locus_tag="VFX38_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine synthase"
                     /protein_id="HEX5314932.1"
                     /translation="MIVKTLQDIVGTDADVSAPTWNSRRLLLKRDGMGYSIHDTIIRA
                     GTETHIHYRHHLEAVYLIEGAGEVETVADGKVYPLAAGTLYALDAHDEHYLRANKGTH
                     MRMVCVFNPPLTGDEVHDDSGVYALPAEAKTAA"
     gene            1230..2138
                     /gene="thpD"
                     /locus_tag="VFX38_08535"
     CDS             1230..2138
                     /gene="thpD"
                     /locus_tag="VFX38_08535"
                     /EC_number="1.14.11.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002645660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine hydroxylase"
                     /protein_id="HEX5314933.1"
                     /translation="MNANAHSDESLADLYPSRVGGEHRFLPRRDAIVYGSATEGPLDG
                     ATLARYERDGFLHLPEFLDNDDIAPARAELERLREDHAERARPDAITEPGSDALRSLF
                     AVHFDRGAVAALCRERRLVAIARQLLGGDVYILQSRINYKPGFGGREFFWHSDFETWH
                     AEDGLPRMRTASCSIALTANDANNGPLLLVPGSHRRFLSCAGETPEDNYKSSLKRQEL
                     GVPDEAHLGELIGQGGLAAATGPAGSATFFDCNLMHGSNSNITPRPRSNIFVVFNSVK
                     NRLSRPFGGRDPRPEFLAHRLRAEPL"
     gene            complement(2154..3146)
                     /locus_tag="VFX38_08540"
     CDS             complement(2154..3146)
                     /locus_tag="VFX38_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX5314934.1"
                     /translation="MSTYVGIDVGAKRCEWAWREAGCAREGGSFEQNPGGHAAVIARL
                     KRLVPERVVLEATGVYYLDLALALHAAGLPVAVINPKSFHHFVQLKLKVSKTDRLDAG
                     LLAEYAERMRPALWTPPEPTCLALRDIGRQIGRLTHMRTQAKNRLHALTAKCATPRLL
                     INDERAGIRALDRRARLSQAGDELIAQAPALAKHFACLRTAKGIGRISAVALLGELCV
                     LPEEMKTAQLARHAGLDVRLTQSGTSVNKPGRLSKSGNRYLRAALYMPALSAVRYDPH
                     ARAFYHALIARGKEKLQALCAVMRKYLTGLWACLKAGEPFDSSRLFSSIHIQGA"
     gene            complement(3671..4597)
                     /gene="ubiA"
                     /locus_tag="VFX38_08545"
     CDS             complement(3671..4597)
                     /gene="ubiA"
                     /locus_tag="VFX38_08545"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEX5314935.1"
                     /translation="MHAPENSRNDVSASPQRLGIRLLRRLGAYARLARMHRPIGTFLL
                     LWPTLWALWIAGRGHPTTLHFAVFVAGTFLMRCAGCAINDFADRGIDSQVRRTRDRPL
                     ARGEIRPAEAVAVFVVFAIAAFFLAFFTLDRLALLLAIPGVLLAASYPFVKRITHLPQ
                     LYLGVAFGWGIPMAFAAEAHALPPVCWLLLGVNVLWATVYDTFYAMADRPDDERIGVR
                     STAILFGRADRTIIAVLQLLVLIGLAVAGWWRELGAVYYWGLGAAAGFALYQQWLARN
                     REPARCFAAFLNNNWFGAAVFAGIILQANLRV"
     gene            complement(4584..>5777)
                     /locus_tag="VFX38_08550"
     CDS             complement(4584..>5777)
                     /locus_tag="VFX38_08550"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014038333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX5314936.1"
                     /translation="LQGDVGSGKTAVAALAVAQALGAGFQAALMAPTELLAEQHARNL
                     RPWFDPLGLPLGLVTAATPAALRREWTGRAAAGEPLFLIGTQALLSRDVRFARLALAI
                     VDEQHRFGVDQRLALAASGEGPVPHRLVMTATPIPRTLAMTLYADLDVSTLDERPPGR
                     KPVVTAVMSEERRDELVARIARLVAAGQQTYWVCPLIEESELLDAQAAEQSATTLAEA
                     LPGARVALLHGRLGAAQKDAIMRRFVAGEIDVLVATTVIEVGVDVPNASLMVVENAER
                     LGLAQLHQLRGRVGRGAAASHCILLYKPPLSAQARERLAALRASDDGFVIAEADLKLR
                     GPGEFAGTRQAGLARLRVADLVRDRGLLARVAPVANRLLAEEPAEADALIRFWIGAES
                     RYARA"
BASE COUNT         1039 a         1965 c         1864 g          909 t
ORIGIN      
        1 ccctacgcgg gttatctcgg cgacggcggc gataccctcg cctatctcga gaccgtactc
       61 gccgatcgcg cgagcggcgt cgacaagccc gccgccatca tcgtggaaac ggtgcaggcc
      121 gaaggcggca tcaacgtcgc gagcgccgga tggttgcgcc gtctgggcgc gatcgctcgc
      181 gcaaacgacg tgctcctgat cgtggacgac atccaggttg gatgcgggcg caccggcccg
      241 ttcttcagct tcgaggaagc cggaatcgtc ccggacatgg tgacgctttc gaaggcgctt
      301 tcgggctacg ggctgccgct cgcggtcgtg ctcatccgtc ccgaactcga tcaatgggaa
      361 ccgggcgaac acaacgggac cttccgcggg cacaatcccg cgttcgtgac cgccgcagcg
      421 gcgctggact tctggcgcga cgacgcgctg gaactcgcaa cctgcgagaa ggccgcggcc
      481 cttcgcgctg ccctcgagaa catcacggat gcgaatgacg gcatcgccga agtgcgcggc
      541 cgcggcctca tccaaggtct cgcgttccgc gagcccggac tcgccaacct ggtttcggcc
      601 gaggccttcc ggcgcgggct cgtcatcgaa accgcggggg ccgaaagcga ggttctcaag
      661 tgcctgccgc cgctcacgat cggcgccgac gaactcggcg agggtatcga catcatcgcg
      721 gacagcgtcg ccgccgcgac gacctcgcgt cgccgcgacg cggcctgact ctttccattc
      781 cttcactcac gggaacgctt acatgatcgt aaaaaccctg caagacatcg tcggcaccga
      841 tgccgacgtc tccgcaccga cctggaacag ccgccgcctg ctcctgaagc gcgatggcat
      901 gggctattcg attcacgaca ccatcattcg cgcaggcacc gaaacgcata tccattaccg
      961 ccatcatctc gaagcggtgt atctgatcga aggcgccggc gaggtcgaaa ccgtggccga
     1021 cggcaaggtg tatccgctcg cggccggcac cctgtacgcg ctcgacgccc acgacgaaca
     1081 ttacctgcga gccaacaagg ggacgcacat gcgcatggtc tgcgtcttca atccgccgct
     1141 cacgggcgac gaagtgcatg acgattccgg cgtgtatgcc ctgccggcgg aagccaaaac
     1201 cgcggcctga gccggtttgc cgaaccgaca tgaacgccaa cgcacattct gacgaaagcc
     1261 ttgcggatct ctacccgtcg cgcgtcggcg gcgagcaccg atttctgccg cgccgcgatg
     1321 cgatcgtgta cggcagcgcg accgagggac cgctcgacgg cgcgacgctg gcacgttacg
     1381 agcgcgacgg ctttctgcat ctgcccgagt ttctcgacaa cgacgacatc gcgccggcac
     1441 gggcggaact cgagcgtctg cgggaagatc acgcggagcg tgcgcggccc gacgccatca
     1501 ccgagccggg aagcgacgcc ttgcgctcgc ttttcgccgt gcatttcgac cgtggcgccg
     1561 tcgccgcttt gtgtcgcgag cggcgtctcg tcgcgatcgc tcgccagttg ctgggcggcg
     1621 acgtctacat cctgcaaagc cgcatcaatt acaagccggg cttcggcggg cgcgagttct
     1681 tctggcattc ggattttgaa acctggcatg cggaagacgg cctgccgcgg atgcgcacgg
     1741 caagctgctc gatcgcattg accgcgaacg atgccaacaa cgggcccctg ttgctcgtgc
     1801 ccggttcgca tcggcgcttt ctttcctgcg cgggagaaac tcccgaagac aactacaaaa
     1861 gctcgctcaa gcgccaggaa ctcggggttc ccgacgaagc gcatctcggc gaattgatcg
     1921 ggcaaggcgg cctcgcggct gcgaccgggc cggcgggaag cgcgaccttc ttcgactgca
     1981 acctcatgca cggctccaat tccaacatca cgccgcggcc gcgaagcaac atcttcgtcg
     2041 ttttcaacag cgtgaaaaac cgcctgtcgc gtcccttcgg cggacgcgat ccccgcccgg
     2101 agtttctcgc gcatcggctg cgcgccgaac cattgtagat actctgtttc aagtcaagcc
     2161 ccctgtatgt ggatggagct gaacaaacgc gaggaatcga agggttctcc tgccttgagg
     2221 caggcccaca agccggtgag gtatttgcgc attaccgcgc acagggcttg cagcttctct
     2281 ttgccgcgag cgatgagcgc gtgatagaag gcacgggcgt ggggatcgta gcgtacggcg
     2341 ctgagcgcgg gcatgtacag cgcggcgcgc aggtagcggt tgccgctctt gctcaatcgt
     2401 cccggtttat tcacactcgt gccggattgg gtgaggcgca catcgagccc ggcatggcgg
     2461 gcgagttgag cggttttcat ctcctcgggc aggacgcaca actcgcccag gagcgccacg
     2521 gcacttatgc gcccgatccc cttggccgtg cgcagacacg cgaagtgctt tgccagcgcg
     2581 ggtgcttggg cgatgagttc atcgcccgct tggctgagac gggcgcgccg atcgagcgcg
     2641 cggataccgg cacgctcatc gttgatgagg agtcgtgggg tggcgcactt ggccgtcaag
     2701 gcgtgcaggc gatttttggc ttgggtacgc atgtgggtga gccgcccgat ctgacgcccg
     2761 atgtcgcgca gcgcgaggca cgtgggctca ggcggcgtcc acagcgccgg gcgcatgcgc
     2821 tcggcgtact cggccagcaa ccccgcatcg agccgatcgg tcttggagac cttaagcttg
     2881 agctgcacga agtggtgaaa gctcttgggg ttgatcaccg ccacgggcaa ccccgccgcg
     2941 tgcaacgcga gtgccaagtc caggtaatac accccggtgg cctccagcac cacccgctcg
     3001 gggacgaggc gcttcagacg cgcgattacc gcggcgtgtc cgccggggtt ctgctcgaag
     3061 ctcccaccct cgcgcgcgca tcccgcctcg cgccacgccc actcgcagcg cttggcgccc
     3121 acatcaattc cgacatacgt actcatgatc gctttcctct tggttcgggt ttgggccaca
     3181 atagtcaccg gctccccgac ctcgcattga cggctcgcgc gaccttgtca tacgaagtcc
     3241 gctcaccagc ggcttcccga tactcctcgc gctgggccat gaggcgggcg gccactctac
     3301 atgcgaggtc agacaatgct gcctcaagga gcgaacggct tccccggtga actctcaact
     3361 tcggacgtcc tgacctctcg gcacggccga tcgctttaac atacaaggag cgagcgtgct
     3421 cgcgatctgg cggctcctca tcaccatccg gctcgctccc gcatccgcct gcgatgcaca
     3481 cacatacaat ctcgcgtagg ccggcaaaag gccgaagagc gttgccggca tttcggaatc
     3541 gccgggaacg ctccgcttgt ccggcctccc ttccgacagc gtcaccgcgc cgcgcagccg
     3601 cttgcgcacg gtttcgcaca gcatccggat cggtccttgc cgcaaaaaca cgaagcggac
     3661 gacgaagcgc tcagacgcgg agattcgcct gcaggatgat cccggcgaag accgccgcgc
     3721 caaaccagtt gttgttcagg aacgcggcga aacagcgcgc gggctcgcga ttgcgcgcga
     3781 gccattgttg atagagagcg aagccggcgg ctgcgccgag gccccagtaa taaacggcgc
     3841 cgagttcgcg ccaccatccc gctaccgcga ggccgatcag caccagaagt tgcagcaccg
     3901 cgatgatcgt gcggtctgcc cggccgaaaa gaatggccgt ggagcgcacg ccgatacgct
     3961 cgtcatcggg gcggtcggcc atcgcgtaga aggtgtcgta gaccgtcgcc cagagcacgt
     4021 tcacgcccag cagcagccag caaaccggcg gcagcgcatg cgcttcggcg gcgaaggcca
     4081 tcggaattcc ccagccgaac gctacgccga gatagagctg cggcagatgc gtgatgcgct
     4141 tcacgaacgg atagctcgct gccagcagaa cgccgggaat ggcaagcagc aaggcgagtc
     4201 ggtcgagggt gaagaacgcg agaaagaacg cggcgatcgc gaacacgaca aacaccgcca
     4261 ccgcttccgc cggacgaatc tcgccccgcg cgagcggccg atcgcgggtg cgccgaacct
     4321 gtgaatcaat cccgcggtcg gcaaagtcgt tgatcgcgca accggcgcag cgcatgagga
     4381 acgtgccggc gacgaacacc gcgaagtgca gcgtcgtcgg gtggccgcgc cccgcgatcc
     4441 acagagccca gagcgtcggc cagagcagca gaaacgtgcc gatgggccgg tgcatgcggg
     4501 caagccgggc ataggcgccg agacgccgca acagccggat tcccaagcgt tgcggcgagg
     4561 ccgacacgtc gttgcgcgaa ttttcaggcg cgtgcatagc gcgactcggc accgatccag
     4621 aaacggatga gggcgtcggc ttcggcgggt tcttccgcga gcaggcggtt tgcgaccggc
     4681 gccacccgcg cgagcaggcc gcgatcgcgg acgagatcgg ccacgcgcag ccgcgcaagc
     4741 cccgcctgcc gggttcccgc aaattcgccg ggaccgcgaa gtttgagatc cgcttcggcg
     4801 atgacgaaac cgtcgtcgct cgcgcgcagc gccgcgaggc gttcccgcgc ctgcgcggaa
     4861 agcggaggct tgtagagcag gatgcaatgg ctggccgcgg cgccgcgccc cactcttccg
     4921 cgcagttgat gcaactgcgc gagaccgaga cgctcggcgt tctccactac catgaggctc
     4981 gcgttcggca cgtccacgcc aacttcgatc acggtagtcg cgaccaggac atcgatttcg
     5041 ccggcgacga agcggcgcat gatcgcatcc ttctgcgccg cgccgagccg cccatgcagc
     5101 agcgcgaccc gggcgccggg aagcgcctcg gccaaggtcg tcgcgctctg ttcggcggcc
     5161 tgcgcatcga gcagttcgga ctcctcgatc agcggacaga cccagtacgt ctgctgaccg
     5221 gcggcgacga gccgggcgat gcgcgcaacg agttcgtcgc ggcgctcctc gctcatgacg
     5281 gcggtcacga ccggcttgcg gcccggcgga cgttcgtcga gcgtggaaac atcgagatcg
     5341 gcatacaggg tcatcgcgag cgtacgggga atcggcgtcg ccgtcatgac gagacgatgc
     5401 ggcaccggtc cctcgccgct cgcggcaagc gcaaggcgct gatccacgcc gaagcgatgc
     5461 tgctcgtcca cgatcgcgag cgcgagacga gcgaagcgaa cgtcacggga aagcagcgcc
     5521 tgcgtcccga tgagaaacag cggctcaccc gccgcggccc gcccggtcca ttcgcgtcgc
     5581 agcgccgccg gcgtggcggc ggtgaccaga ccgagcggca gtccgagcgg atcgaaccat
     5641 gggcgcaggt tgcgcgcatg ctgctccgcc agcagttccg tcggtgccat cagggccgcc
     5701 tggaagccgg ctcccagcgc ttgcgcgacg gcgagcgccg cgaccgccgt cttgccgctt
     5761 ccgacgtcgc cctgaag
//
LOCUS       DASUJG010000402         2251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_115877, whole genome shotgun sequence.
ACCESSION   DASUJG010000402 DASUJG010000000
VERSION     DASUJG010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115877"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VFX38_08555"
     CDS             complement(<1..337)
                     /locus_tag="VFX38_08555"
                     /inference="COORDINATES: protein motif:HMM:NF018299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314937.1"
                     /translation="MSESSPMRLLRGRIKPAVFTGSVVTIGKFDGLHRGHRALLARTG
                     EQASLLALPAVLLSFEPLPPEFFSRAKARLSSFAQKWRLLADTATIDTLACLRFDRAL
                     AALSAEDFVA"
     gene            complement(330..1898)
                     /gene="murJ"
                     /locus_tag="VFX38_08560"
     CDS             complement(330..1898)
                     /gene="murJ"
                     /locus_tag="VFX38_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX5314938.1"
                     /translation="MRGGFLANLSTVGGFTLLSRILGFARDIVFATHFGAGYGMDAFL
                     VAFKIPNFGRRLFAEGAFSQAFVPVLAEYRATRPESEVRELVSRTEGSLAGALALLSA
                     FGVLLTPVFIWIFAPGFHAIPGKFELATDLLRLTFPYLFFISLVALAGGVLNAHGSFG
                     PPAFAPVLLNIAFIVAALFVAPHLAQGSYALAGAVLVAGALQFAMQLPYLARHGMLVL
                     PRLGWRDPGVRRIFRLMVPVMFGASVTQLNLLVDTVIASFLASGSVSWLYYSDRLMEF
                     PLGVFAVALGTIILPGLAGHHVRGEHDRYSAMLDRALRLTAFAMPAAAIALIVLAGPL
                     VATLFGYGKFSDHDVAMTRASLVTYAAGLTAFSLVKVLLPAWYARQQTRKPVRIGILA
                     VGVNLVFNGALVVPWALTGGVAPHAGLALATSLAAVVNAALLWRGLRGIYRPLRGWTP
                     FLLRIAAGCVAMAAGLVWAQSRLSQWLNWRAEERVLHLVLLVLGGACLYLVVLGLAGL
                     RPRHLRLGAGEPDV"
     gene            1984..>2251
                     /gene="rpsT"
                     /locus_tag="VFX38_08565"
     CDS             1984..>2251
                     /gene="rpsT"
                     /locus_tag="VFX38_08565"
                     /inference="COORDINATES: protein motif:HMM:NF013787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX5314939.1"
                     /translation="MANTAQARKRARQAEERRARNQQLRSRVRSAIKKVEKATAGGDA
                     AAARAALQAATPEIDTMAGKRILKKNAASRYKHRLNLAIRRLSTP"
BASE COUNT          459 a          737 c          789 g          266 t
ORIGIN      
        1 ctgcgacgaa gtcctccgcg gaaagggcgg cgagcgcgcg gtcgaatcga aggcaggcca
       61 gggtgtcgat cgtggccgtg tccgcgagca gtcgccattt ctgcgcgaag ctcgaaaggc
      121 gcgccttcgc ccgggaaaag aattccggcg gcaacggttc gaagctcagc aggaccgcgg
      181 gcagcgcgag caggcttgcc tgctcgcccg tgcgcgcgag caaggcgcga tgcccgcgat
      241 gcaggccgtc gaacttgccg atcgtaacta ccgagcccgt gaagaccgca ggtttgattc
      301 gtccgcgcag caggcgcatc ggcgaagatt cagacatcgg gctcgccggc gccgagacgc
      361 aaatggcggg ggcgcaatcc ggcgagtcca agcacgacga ggtacaggca ggccccgccg
      421 aggacgagca gcacgaggtg aagcacgcgc tcctccgccc gccagttcaa ccattgcgag
      481 agtctcgact gcgcccagac gagccccgcg gccatcgcga cgcagccggc ggcgattcgc
      541 aagaggaaag gcgtccagcc gcgaagcggc cggtatatgc cgcgcaggcc gcgccacaag
      601 agggccgcgt tcacgaccgc ggcgagcgag gtcgccagcg ccagtcccgc gtggggcgcg
      661 actccgccgg tgagcgccca gggaacgacc agggcgccgt tgaacacgag attgactcca
      721 acggcgagga tgccgatgcg cacgggcttg cgcgtctgct ggcgcgcata ccaggccgga
      781 agcagcacct tgaccagcga gaacgccgtc aatcccgccg catacgtcac gaggctcgcc
      841 cgcgtcatgg ctacgtcgtg atcgctgaac ttgccgtaac cgaagagcgt cgcgacgaga
      901 ggtcccgcga gcacgatgag ggcgatcgcg gcggcgggca tggcgaaggc ggtgaggcgc
      961 agggcgcggt ccagcatggc cgagtagcga tcgtgctcgc cgcggacgtg atggcccgcg
     1021 agccccggaa ggatgatggt cccgagcgcg accgcaaaga ctccgagcgg aaactccatc
     1081 agccgatcgg agtaatacag ccagctcacg ctgccgctcg cgagaaacga tgcgatcacg
     1141 gtgtccacga gaaggttcaa ctgcgtcacc gaggcgccga acatgaccgg caccatcagg
     1201 cggaaaatgc gccgcactcc cggatcgcgc catccgaggc gcggcagcac cagcatcccg
     1261 tggcgcgcga ggtagggaag ctgcatcgcg aattgcaagg cgcccgcgac cagcacggcg
     1321 cccgcgagcg catagctccc ctgggcgaga tgcggcgcga cgaacagcgc cgcgacgatg
     1381 aaggcgatgt tgagcaggac cggcgcgaac gcaggcgggc cgaaactgcc gtgggcgttg
     1441 agcacgccgc cggcgagcgc cacgagcgag atgaagaaca ggtagggaaa ggtcaggcgg
     1501 agcagatcgg tcgcgagttc gaacttgccg gggatggcgt ggaagccggg cgcgaaaatc
     1561 cagatgaaca ccggggtgag aagcacgccg aaggcgctga gcagcgcgag cgcgcccgcg
     1621 aggctgcctt ccgtgcgcga caccagctcg cgcacctcac tctccggacg cgtcgcgcga
     1681 tattcggcga gcaccggcac gaaggcttgg gaaaacgccc cttcggcgaa gaggcggcgg
     1741 ccgaagttcg gaatcttgaa cgcgaccaga aaggcgtcca tgccgtagcc ggcgccgaag
     1801 tgcgtcgcga acacgatgtc gcgggcgaag ccgagaatgc gtgacagcag cgtgaagccg
     1861 ccgacggtgg agagattcgc gagaaagccg cctctcatgg tttcatgcgc gagcgcccgg
     1921 cttgagcccg gcccggtttc gcggcatact atgcggccca gcgaaagttt tcacggagac
     1981 tccgtggcca ataccgccca ggcacgcaag cgcgcccgtc aggccgaaga gcgccgcgcc
     2041 cgaaatcagc agttgcgctc gcgcgtgcgc agcgccatca agaaagtcga aaaggcgacg
     2101 gccggtggcg atgccgccgc tgcccgtgcc gccctgcagg cggcgacgcc cgagatcgac
     2161 accatggccg gcaagcgcat cctgaagaaa aacgccgcat cccgctacaa gcatcggctg
     2221 aatctcgcca tcaggcgtct ttcgactccg c
//
LOCUS       DASUJG010000403         2238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_727113, whole genome shotgun sequence.
ACCESSION   DASUJG010000403 DASUJG010000000
VERSION     DASUJG010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727113"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..559)
                     /locus_tag="VFX38_08570"
     CDS             complement(173..559)
                     /locus_tag="VFX38_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEX5314940.1"
                     /translation="MQNLIRNSAFALAAAGMAVSVPAAARDHGHHNRYEQTYRDRYGY
                     DTRYYGRDYHSRADYRDARSWRGNDGRYYCRKSDGTAGLVVGAVAGALLGRAVDGGYD
                     HTLGTVVGGAIGALLGKSVAQHNTCR"
     gene            complement(789..1817)
                     /gene="pyrC"
                     /locus_tag="VFX38_08575"
     CDS             complement(789..1817)
                     /gene="pyrC"
                     /locus_tag="VFX38_08575"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008601762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX5314941.1"
                     /translation="MAPPETLTLRRPDDWHVHLRDGEMMRALLPYTARQFARAIVMPN
                     LSPPVTDTAAARAYRERIMAALPDGANFTPLMTAYLTDGTDPADLARGFEEGVFTAAK
                     LYPANATTNSAHGVTDVSHIRHVLERIVDIGMVLCVHGEVTDPEVDVFDREVVFIDRV
                     LAPLLRELPELRVVLEHVTTGHAVQFVEDSPATVAATITPQHLHLNRNAMLVGGIRPH
                     AYCLPVAKREKHRQALRQAATSGNPKFFLGTDSAPHLRGAKESACGCAGIFNAPFALE
                     SYLMVFEAEGALDKFAGFASEHGTRFYGLPLNEGTVTLVREPSEVPDEIAGVVPFHTG
                     EMLAWKMA"
     gene            1856..>2238
                     /locus_tag="VFX38_08580"
     CDS             1856..>2238
                     /locus_tag="VFX38_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HEX5314942.1"
                     /translation="MPAERLFSRAIIRLSPQEENEDVADFLQGLVTQDVKDELPAWAG
                     LLTPQGKTLFDFIVWPAGRGELLLDCEADAADDLIRRLSMYRLRRKIGIARDDTLGVY
                     WQPELGDGGAPDPRLHELGQRWIAKA"
BASE COUNT          383 a          722 c          759 g          374 t
ORIGIN      
        1 aaccgcccgg cgtcccgatt cgggaaaggc cacccgcggg tgtaaacgcc gcataaaagc
       61 gaggcgccca tgcggttccg catgggcgcc tcggcgagac cctcgaaaat ttgcgacccc
      121 cgaaggcctc gctgtggccg cacccaacag ggaaaggcgc ggccggactg gctcagcggc
      181 aggtgttgtg ctgcgcaacg ctcttgccga gcagcgcgcc gatggcgccg ccgaccacgg
      241 tgccgagcgt gtggtcgtag cccccgtcga ccgcgcggcc gagcaaggca ccggccacgg
      301 cgccgaccac caggccggcc gtgccgtcgc tcttgcggca gtaatagcgc ccgtcgttgc
      361 cgcgccagga gcgagcgtcg cgataatcgg cgcgcgagtg gtaatcgcga ccgtaatagc
      421 gggtgtcgta gccgtagcgg tcgcgatagg tctgctcgta gcgattgtga tgaccatggt
      481 cgcgggcggc ggcgggaacg gacaccgcca tgccggcggc ggcaagcgcg aaggcgctgt
      541 tgcggatgag gttctgcatg gttttgctcc tttcggacga ggtcgtccgt tgcgttcatg
      601 tttagggggc ttttcatgaa cacatctgca acgaggttgt cagaaatagc aaatatctga
      661 atttacacga tttattccga aaatcagccg ctcgtgggca cttgcggccg cccgtaatcg
      721 tttgacaatg agcggatcgc gcacggtcac gagtggcgcg caaaatggaa agcgccggat
      781 tcggtcgcct aggccatttt ccaggcgagc atttcgccgg tgtggaacgg cacgacgccg
      841 gcgatctcat cgggcacttc ggacggctcg cgcaccagcg tgaccgtgcc ctcgttgagc
      901 ggcaaaccat agaatcgcgt cccgtgttcg gaggcgaacc ccgcgaactt gtcgagtgcg
      961 ccttcggcct cgaacaccat cagatagctc tcgagcgcga acggcgcgtt gaatatcccc
     1021 gcgcaaccgc acgcgctttc cttggcgccg cgcaaatggg gggcgctgtc ggtcccgaga
     1081 aagaacttcg gattgcccga agtcgccgcc tggcgcagcg cctgccggtg cttctcgcgc
     1141 ttggcgacag gcaggcaata agcgtggggc ctgatcccgc cgacgagcat cgcgttacgg
     1201 ttgaggtgca agtgctgcgg ggtgatggtc gcggcgacgg tggccggcga atcctcgacg
     1261 aactgcactg catgcccggt ggtgacgtgc tcgaggacga cgcgaagctc cggcagttcg
     1321 cgtagcagcg gcgccagcac gcgatcgatg aacaccacct cgcggtcgaa cacatcgacc
     1381 tcggggtcgg tcacttcgcc gtggacgcac agcaccatac cgatgtcgac tatccgctcg
     1441 agcacatggc ggatgtggga gacgtccgtc acgccatgcg ccgaattggt cgtggcgttc
     1501 gcgggataga gcttggccgc cgtgaacacc ccttcctcga acccgcgcgc cagatcggcg
     1561 gggtcggtgc cgtcggtgag ataggcggtc atcagcggcg tgaagttcgc gccgtccggc
     1621 aacgccgcca tgatccgctc gcgataagcc cgcgccgccg ccgtatcggt gaccggcggc
     1681 gaaaggttcg gcatcacgat ggcgcgcgcg aactgccgtg cggtgtacgg cagcagggcg
     1741 cgcatcatct cgccgtcgcg caagtggacg tgccaatcgt cgggccggcg gagggtgagc
     1801 gtttcgggcg gcgccatggc ttccccttag cgagcgcgat gcctatctgt cacccatgcc
     1861 cgccgaacgc ctgttttccc gcgcgatcat ccgcctgagc ccgcaagagg aaaacgagga
     1921 tgtcgccgat ttcctgcaag gtctcgtcac gcaagacgtg aaggacgagc tgccggcgtg
     1981 ggccgggttg ctgacgccgc aagggaagac cttgttcgac ttcatcgtct ggcccgccgg
     2041 ccgcggcgag ctgctgctcg actgcgaggc cgacgcggcc gacgacctga tccgccgcct
     2101 ttccatgtac cgcctgcgtc gcaagatcgg catcgcccgc gacgacacgc tcggcgttta
     2161 ctggcagccc gagctcggcg acggcggcgc gcccgatccg cggctgcacg aattgggcca
     2221 gcggtggatc gccaaggc
//
LOCUS       DASUJG010000404         4187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_362200, whole genome shotgun sequence.
ACCESSION   DASUJG010000404 DASUJG010000000
VERSION     DASUJG010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362200"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1614
                     /locus_tag="VFX38_08585"
     CDS             <1..1614
                     /locus_tag="VFX38_08585"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX5314943.1"
                     /translation="RGVRITNASDCGGQDCLDNIYTYWRNLSNSAGSNTMYILVGLDR
                     NHGGAGPTLFQFNKTSEALTKVGALFSPSDPLSWASTEGWFFSYSMPTKIYITNDDSK
                     LRRYDVLTHQMTTVFDITNQYPGMAVRQAYASNDDNVFSATLVNASTWESEGCIVYTA
                     SSGQFHFFPKRGDFDECQVDKSGRYVLIFETLPANVCPPADGHDVVEDLQTGEEQILC
                     KQDGAGGHYALGYGWMMEADSYAPLANTWRLWDLAKPLVKGIILPNGEKQGAMVYHDP
                     DWAIIAPDHATWENAVPDSVTPVTAQYNCAGAANRTLGVRSHEVECFPLDPSASAQNE
                     WTYVVAPTMTDMDAPGGRDDYSKYPKGNLDPTGHYFFWLSNLGGDRLDAFMVEIPYQK
                     LGGGGGDPPPPPNPSIAIDSPNPGATLSGTVDISASVSDDSQITRVQYQLDGQDIGSA
                     VTEPPYTMSWDTSATGVGNHTLLAIAFEESGSTVTSSPVSVVVARDGGPPGGGHASGG
                     GSGALTPWVALGLLSLVLASALSRALRRR"
     gene            1920..2483
                     /locus_tag="VFX38_08590"
     CDS             1920..2483
                     /locus_tag="VFX38_08590"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HEX5314944.1"
                     /translation="MPGNYADVHEPAATESLAIDREPQWHVLWAKSNCERSVQEQLAA
                     KGYEVLLPTVQKWSRSSRVQRLRCAPLFPGYLFLRRAIDRRSYLDISRARGLVRILGA
                     GWEHPACVPEREMEAICRLQHSELPRLPYTYLNEGERVRITRGPLVNATGIFLRTDSV
                     KGLLILSVDLLSRSVAVRVNCSHVVPA"
     gene            2742..3956
                     /locus_tag="VFX38_08595"
     CDS             2742..3956
                     /locus_tag="VFX38_08595"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX5314945.1"
                     /translation="MAKATLILGTGTLAQQVAVGLGSHRVRGYRLLGEIGEGPRAGSE
                     DLAGPLLGTTFDLPRVVAERRIRRLIVAPADPAAPLPLDLLIGTRTGRRLSIESGGEV
                     LEKLTGRIAIASMPQTAVLASLLQRPRRSSLLFSRMMSVAIALAALAVLAPLMALIAL
                     AIRIDSRGPILFVQERIGRDTRRFKFLKFRSMFHSTPRRSEWACDNSAEITRVGRFIR
                     RFRLDELPQFLNVLRGDMNIVGPRPHPASNRELLVLVARNASGGGEPTPYYSLRQSVL
                     PGITGWAQVRYKYANGLDEEIEKLCYDLYYVKHCSPLLDLRILFETVKVVLRGGADAA
                     GAPGAPSPAVVPTVAEEETSFRSSPRPMDARRRQGAEFRLCELESDDAGRPDRERDGE
                     ARAGSAPGNGRG"
     gene            3953..>4187
                     /locus_tag="VFX38_08600"
     CDS             3953..>4187
                     /locus_tag="VFX38_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HEX5314946.1"
                     /translation="MNLLERADQSAPRGDGSLAAAAADAPRALAITILGGGHGLAVVL
                     EGLRRSRLAGGEAAASPRVCAVVNMADDGGSSGG"
BASE COUNT          700 a         1409 c         1337 g          741 t
ORIGIN      
        1 cgcggggtgc gcatcaccaa tgcgagcgac tgcggcggcc aggactgcct cgataacatc
       61 tacacctact ggcgcaatct gagcaacagc gccggcagca acacgatgta cattctcgtc
      121 gggctcgatc gcaaccacgg cggagcgggc ccgacgctct tccagttcaa caagacgagc
      181 gaggcgctca cgaaggtggg ggcgctgttc tcgccctccg atcccctgag ctgggcctcg
      241 accgagggct ggttcttcag ctactcgatg ccgaccaaga tctacattac gaacgacgac
      301 tcgaagctcc ggcgctatga cgtgctcacg caccagatga cgacggtatt tgacatcacc
      361 aatcagtacc cggggatggc cgtgcgccag gcctatgcga gcaacgacga caacgtgttc
      421 tcggcgacgc tggtgaacgc gagcacctgg gagtccgagg gctgcattgt ctataccgcc
      481 tcgagcgggc agttccactt ctttcccaag cgcggggact tcgatgagtg ccaggtggac
      541 aagagcgggc gctacgtgct gattttcgag accctgcccg cgaacgtgtg cccgcccgcc
      601 gatggtcacg acgtggtgga ggatctccag acgggagagg agcagattct gtgcaagcag
      661 gacggggcgg gcggccacta cgcgctcggc tacggctgga tgatggaggc cgactcctac
      721 gccccgctcg ccaatacctg gcggctgtgg gacctcgcca agcccctggt gaaggggatt
      781 atcctgccca atggcgagaa gcagggcgcg atggtctacc acgatcccga ctgggcgatc
      841 attgcccccg atcatgccac ctgggagaat gccgtacccg attccgtgac gcccgtcacc
      901 gcgcagtaca actgcgccgg ggcggccaac cgcacgctcg gagtgcgctc ccacgaggtc
      961 gagtgcttcc cgctcgatcc ttcggcctcg gcgcagaacg agtggaccta cgtcgtcgct
     1021 ccgacgatga ccgacatgga tgcgccgggc ggccgcgacg actactcgaa gtatccgaag
     1081 ggcaatctcg atccgaccgg ccattacttc ttctggctga gcaatctcgg cggcgaccgc
     1141 ctcgacgcct tcatggtcga gattccgtat cagaaattgg gcggcggagg cggcgacccg
     1201 ccgccgcccc caaacccgag catcgccatt gactcgccga atccgggcgc gaccttgagc
     1261 ggcacggtcg atatctcggc atccgtcagc gacgatagcc aaattacccg cgttcagtac
     1321 caactcgacg ggcaggacat cggatcggca gtgacggagc cgccctacac gatgtcctgg
     1381 gatacctcgg ccactggcgt cggcaaccac accctgctcg ccatcgcatt cgaggaatcc
     1441 gggagcaccg tgacttcctc gccggtttcg gtggtcgtcg cgcgcgacgg cggccctccc
     1501 ggcggcggcc acgcgtccgg cggaggctcg ggtgcactca cgccctgggt tgcgctcgga
     1561 ctcttgtctc tcgtcttggc ctcggccctc tcgcgagcct tgcgccgacg ctgagtccgg
     1621 ccgtttgcgg cggtccggaa gggccgccga tcgcgcgacg attttttcct ccttctgccg
     1681 ctgcgaagcg tttcgacgct tcgcagcggt cttcgcgtcg ccgggcggga gaattcaata
     1741 aggatttccg cccgcgacaa gaagggaatt ccccgattgg ccgcgcatcg gctgcatggc
     1801 gtaatgagtg ctcggactct gtcctgcgtg cctgaagcgg atctccgcgg tcgcacgggg
     1861 gcagcgccga acgattcacg caagcggctt cgggagatcg aagccgaagg ggcgaagcct
     1921 tgccgggaaa ctacgccgac gttcacgagc cagcggcaac ggagtcgctg gcgatcgacc
     1981 gcgagccgca atggcacgtg ctatgggcga aaagcaactg cgagcgctcg gtgcaggagc
     2041 aactcgcggc gaagggttat gaagttcttc ttcccaccgt gcagaagtgg tcccgcagca
     2101 gccgcgtcca acgcctgcgt tgcgccccgc tctttccggg ttatctcttc ctccgccgcg
     2161 cgatcgatcg gcgcagttat ctcgacattt cccgggcgcg cggcctcgtg cgcatcctcg
     2221 gcgcgggttg ggagcacccg gcctgcgtac ccgagcgcga gatggaggcg atttgccgct
     2281 tgcagcactc ggagcttccc cgcctgcctt atacctacct gaacgagggg gagcgcgtgc
     2341 gcatcacccg cggaccgctg gtgaacgcga cgggtatctt tctcaggacc gattccgtca
     2401 agggacttct gatcctttcg gtcgatctcc tctcgcgcag cgtcgccgtc cgggtgaact
     2461 gttctcacgt cgtcccggcg taaagaagtt ctgatttatt ccgtcatacc ggcgccggcc
     2521 ggtatccagt gaacgaggca tcccggcttc tggccgggga tacagactcg tgccggaccc
     2581 cggttttcgc cggggtgacg ggcaaggcaa tggatccgag gttcctcaat gaggtttccg
     2641 gcaccttgtc gcatcgccga caaccctcgc ggtgttcgcg tcgctggcac ctcggaaatc
     2701 aattcatcgc atcggagagc gtcgctcagg agcggacgtc gatggcaaag gctacgctga
     2761 ttctcggcac cggaaccctg gcgcaacaag tcgccgtcgg gctcggatcg catcgcgtgc
     2821 gcggctatcg gcttctcggg gagatcggcg agggtccgcg agccggctcc gaggatttgg
     2881 ccggtcctct cctcggaacg acattcgacc tgccgcgggt cgtcgccgag cgccgcatac
     2941 gacgcctcat cgtggcgccc gccgatccgg ccgctccgct tccgctcgat ctgttgatcg
     3001 ggacgcggac cggacggcgc ctcagcatcg agtccggcgg cgaagtgctc gagaaattga
     3061 ccggacgaat cgccatcgcg tccatgccgc aaactgcggt tctcgcctcc ctgctccagc
     3121 gcccgcgccg ctcgagcctg cttttctcgc gcatgatgag cgttgccatt gccctcgccg
     3181 cgctcgccgt cctggcgccg ctgatggcgc tgatcgcgct cgcgatccgc atcgattcgc
     3241 gcgggccgat cctctttgtg caggaacgca tcggccgcga cacgcggcgg ttcaaattcc
     3301 tcaagtttcg cagcatgttc cactccactc cccggcgatc ggaatgggcc tgcgacaaca
     3361 gcgcagaaat tacccgggtg ggacgcttca tccgccggtt tcggctcgac gaactcccgc
     3421 aattcctcaa cgttcttcgc ggcgacatga atatcgtcgg cccgcgtccg catccggcga
     3481 gcaatcgcga actgctggtg ctggtcgcga gaaatgcctc cggaggaggc gagccgactc
     3541 cctattattc gctgcgtcag tcggtgttgc cgggcatcac cggctgggcc caggtgcgct
     3601 acaaatacgc caacgggctc gacgaggaaa tcgaaaaact ctgctacgac ctgtactacg
     3661 tcaaacattg ctcgccgctc ctcgatttgc gcatcctctt cgagaccgtc aaggtggtat
     3721 tgcgcggcgg cgccgacgcc gccggagcgc ccggtgcgcc gtcgccggcg gtcgtgccaa
     3781 ccgtcgccga ggaagagacg agcttccgct cctcaccccg gcccatggac gcccgcaggc
     3841 ggcaaggcgc ggagttccgt ttgtgcgaac tcgaaagcga cgatgccggc cgacccgacc
     3901 gcgaacgcga tggcgaagcg cgagccggct cggctccggg aaacggacga ggatgaacct
     3961 tctcgaacgc gctgatcagt ctgcccctcg cggggacgga tcccttgcgg cggccgccgc
     4021 ggatgcgccc cgcgcgctcg cgatcaccat ccttggcgga ggtcacgggc ttgccgtggt
     4081 tctcgaaggg ctgcggcgct cgcggcttgc cggcggggag gctgcggcgt cgccgcgcgt
     4141 atgcgccgtc gtcaacatgg ccgacgacgg cggcagctcg ggcgggt
//
LOCUS       DASUJG010000405         2918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1217902, whole genome shotgun sequence.
ACCESSION   DASUJG010000405 DASUJG010000000
VERSION     DASUJG010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217902"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1201)
                     /locus_tag="VFX38_08605"
     CDS             complement(<1..1201)
                     /locus_tag="VFX38_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin"
                     /protein_id="HEX5314947.1"
                     /translation="MLTGELFRLAPRDEKAADASGLRIRKAIACERIEAEPKAARAAA
                     RRGGVALHAVLEDAHDGLTIDWRAVLRDGTNYVREIVQLKPARALDLAKVTMIDLRLA
                     NAWVAGTVDGSPIVAGDRFFGFEQPMAQAAVFGNQATMTLERKLPLRAGVEVTYSAVF
                     GVAPPGQLRRGFLAYLANERAHPYRQFLHYNSWYDIGYFNRYNQTEALREINAIGEQL
                     VEKRGVDMKSFLFDDGWDDPSHLWQFDKGFPQGFAPLRKAAAKYGAGLGIWLSPWGGY
                     GEPREQRLAAARAAGYTVDEQGIALSGPKYYQLFHAVALSLLRDYDIDLFKFDGTGSP
                     DKVTPGSHFDSDFAAAIALIGDLRAVKPDLFVNLTTGTWPSPFWLRYADSIWRGGWDH
                     SFAGVGSQ"
     gene            complement(1502..2470)
                     /locus_tag="VFX38_08610"
     CDS             complement(1502..2470)
                     /locus_tag="VFX38_08610"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314948.1"
                     /translation="MNQRMESTVVDDKEMLAPEFPRVTLNLCRANAPVLVPLVESRIV
                     TAETSPSFVRHVSFDVGGTPLAGQLRAGQSFGVIPPGSDASGKAHKLRLYSVSSPSRG
                     EEGGGRIVSTIVKRVLEEDRDAHRLYLGVCSNYLCDLQIGDPVAMTGPAGKRFLLPVE
                     PDRYNYVFFATGTGIAPFRAMVFDLLAAGVASRIHLLVGVPYRTDYLYHDLFTGPARE
                     LPGFRYRPVVSREERRMDGFKRYVHHEIGDDEELRETLDQPNTLIYICGIKNMEFSVY
                     AELLRAGLGEYLTFRGLPEGMHPTQLRGEELQRHVRPGPRLFLEVY"
     gene            complement(2798..>2918)
                     /locus_tag="VFX38_08615"
     CDS             complement(2798..>2918)
                     /locus_tag="VFX38_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018282284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314949.1"
                     /translation="GKPEVRGRRRMGVGLARADSIAAARAKARAVVAAIAVRI"
BASE COUNT          534 a          942 c          962 g          480 t
ORIGIN      
        1 gctggctgcc cactcccgcg aaggaatggt cccagccgcc gcgccagatg gagtccgcgt
       61 aacgcagcca gaagggcgac ggccaggtgc cggtcgtcag gttgacgaac aggtccggct
      121 tgacggcgcg cagatcgccg atcagcgcaa tcgccgccgc gaagtcgctg tcgaagtggc
      181 tgcccggcgt caccttgtcc ggactgccgg tgccgtcgaa cttgaacagg tcgatgtcgt
      241 agtcgcgcag cagcgagagc gcgaccgcgt gaaacagttg gtagtatttc ggaccggaaa
      301 gcgcgatgcc ctgctcgtcc accgtgtagc cggcggcgcg cgccgccgcg aggcgctgct
      361 cgcgcggctc gccgtagccg ccccagggcg agagccagat gccgaggccg gcaccgtatt
      421 tcgccgcggc cttcctgagc ggcgcgaaac cctgcggaaa gcccttgtcg aattgccaaa
      481 gatgcgaggg atcgtcccag ccgtcgtcga acagaaacga cttcatgtcc acgccgcgct
      541 tctcgaccag ttgctcgccg atcgcgttga tctcgcgcag cgcctccgtc tggttgtagc
      601 gattgaaata gccgatgtcg taccaggagt tgtagtgcag gaactggcgg tagggatgcg
      661 cgcgctcgtt cgcgaggtaa gcgagaaagc cgcggcgcaa ctgccccggc ggcgcgacgc
      721 cgaacacggc cgaatacgtc acttccacgc ccgcgcgcag cggcagcttg cgctcgagtg
      781 tcatcgtcgc ctgattgccg aacaccgcgg cctgcgccat cggctgctcg aatccgaaga
      841 accggtcgcc ggcgacgatc ggcgagccgt ccacggtgcc cgcgacccag gcattcgcga
      901 ggcgaagatc gatcatcgtc accttggcga gatcgagcgc ccgcgcgggc ttcagttgca
      961 cgatctcgcg cacgtaattg gttccgtcgc gcaggaccgc gcgccagtcg atcgtcagac
     1021 cgtcgtgcgc gtcctccaat accgcgtgca gcgccacgcc gccgcggcgg gccgctgccc
     1081 gcgcggcctt cggttccgct tcgatccgct cgcaggcgat cgccttccga atacgcaggc
     1141 cgcttgcgtc cgccgccttt tcatcgcgtg gtgcgaggcg gaacagctcg ccggtgagca
     1201 cagattgttt gccggtgaag ccgttgtcga aggccgcaat tgatcctgcg ccgaggaaac
     1261 ggcgaagcgc agcaccgcat tgtcgagttg tgcatcggct ccctcgacgc tgcaatgcgc
     1321 cggaccggga tcgaccggca gccagggtgt cgccgccagc gacgcgggag tgagcagggt
     1381 aaaggaaagc acgaggaacc gagggcgctt catgacgatc tcccgattgc aagcaacaga
     1441 cggccggtac gacacgtgga atcggccgcg gcaacgcggc atcgaaaaac cggctgcgcc
     1501 gtcaatacac ctcgaggaag aggcgcggac cgggacggac gtgacgctgc aattcttcgc
     1561 cgcggagctg ggtcgggtgc atgccttcgg gaaggccgcg gaaagtcaaa tactcgccga
     1621 ggccggcacg cagcagttcg gcgtacaccg aaaattccat gttcttgatt ccgcagatgt
     1681 agatcagcgt gttcggctgg tcgagcgttt cgcgcagctc ttcgtcgtcg ccgatctcgt
     1741 gatgcacgta gcgcttgaag ccgtccatcc gccgttcctc gcgcgagacc accgggcgat
     1801 agcggaagcc gggcagctcg cgcgccggac cggtaaagag atcgtgatac agatagtcgg
     1861 tgcggtaagg cacgccgacg aggagatgga tgcggctcgc gacgccggcg gcgagtagat
     1921 cgaacaccat cgcgcggaac ggggcgatgc cggtgccggt cgcgaagaac acgtagttgt
     1981 aacggtcggg ttcgaccggc agcagaaaac gcttgccggc ggggccggtc atcgcgaccg
     2041 ggtcgccgat ttgaagatcg cagagataat tcgagcagac gccgagatac agacgatggg
     2101 cgtcgcggtc ctcttccagg acccgcttga cgatcgtgga cacgatccgc ccgccgcctt
     2161 cttcgccgcg cgaaggcgaa ctcacggaat acaggcgcag cttgtgcgcc ttgccgctgg
     2221 catcgctgcc gggcggaatc acgccgaagc tttgcccggc gcgaagttgc cccgcgagcg
     2281 gcgtgccgcc cacgtcgaag ctcacatgac gcacgaaact cgggctcgtc tccgcggtca
     2341 cgatgcgcga ttccaccagc ggcacgagta cgggagcatt ggcccggcaa agattgagcg
     2401 tgacccgcgg gaattcgggc gcgagcattt ccttgtcgtc cactactgtg ctttccatcc
     2461 gttggttcat gtcgtcatgc cgccatcgac gccgggattt ctcgctttcc ggacaagccc
     2521 gcaccgcttg tcgtttccat gagcgggcga cgcttcagcc aatctttcgg cgtgccggtg
     2581 cgcgcctgca cccagggatg cgatgttccg actggattcc ggctttcgcc ggaatgacgg
     2641 agatacgaac aagcgcaaca gtctggcgtt ccaagcgata atagaataac gcaagtgctt
     2701 ggtcggaaca ccgcgcaatt accgcacatt tcgttgcgtg gcaatgaaaa gccttcattg
     2761 cagacgcaag tgcggacgtc aacagagcgc actgcgccta gatgcggacc gcaatcgcgg
     2821 caacgacggc gcgggccttt gcgcgcgccg cggcaatcga atcggcgcgg gcgagcccga
     2881 cgcccatgcg ccgtcggccg cgcacttcgg gcttgccg
//
LOCUS       DASUJG010000406         4288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1707156, whole genome shotgun sequence.
ACCESSION   DASUJG010000406 DASUJG010000000
VERSION     DASUJG010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1707156"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VFX38_08620"
     CDS             <1..388
                     /locus_tag="VFX38_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005958859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX5314950.1"
                     /translation="GALKLKEISYIHAEAYPAGELKHGPLALVDESIPVVAVAPNDET
                     LAKLKSNLSEVRARGGNLIVFTDREARFAPSPDLTLVELSIPQGLLLDPVVYTIPLQL
                     LAYHVAVLRGTDVDQPRNLAKSVTVE"
     gene            537..1679
                     /locus_tag="VFX38_08625"
     CDS             537..1679
                     /locus_tag="VFX38_08625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5314951.1"
                     /translation="MLRVAIIEPVGGHCGMDYYDFGLARGLANAGADVVLHTCDETEI
                     PRDAPFEVRLSYRGIFGEAPKWRRALRYMRGALGALVSAVGERRRIVHFHLFHIRFAE
                     LANIVVARLLGRKVVITAHDVESFVGSLESRRIRKFVYGAADRVIAHNDVSRVELIEN
                     VGVDERKIRQIRHGNFLGLVPELPDSAAARRRVGVSPAARVILYFGQIKEVKGVDVLL
                     RAMPGVVAQFPDAVLLLAGKSWKTDFSRYQAVIDELGIGASCVVHHRFIPPEELPSFF
                     SAADVIALPYRRIYQSGVVLLSMSYGKPVVASDLPGFTQLLEDGVSGYVFSDGDPESL
                     CARLCAALADRSEREAVGRAALEKVIEHYDWDHSGRDTARVYEEIL"
     gene            1676..2842
                     /locus_tag="VFX38_08630"
     CDS             1676..2842
                     /locus_tag="VFX38_08630"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX5314952.1"
                     /translation="MITNRCASFLKVTAAAGAVSLFSLLAAGCMASGANATAPGDSGN
                     AVAPRGTAEIADSTLPPHWPWRGVTMVHGVKNGAQPADVDEFVKAFGINSVHLYPITG
                     KYGPREGLSDAAAWDASMKWLDAMIAACERDHVMAIVHLTSFPKPGGGYYNEHLASFW
                     NNPSAVNEIYTRTAALVKHLGSWGDDVVAFDIISEPTMKTSLGVQAPRDWTKIQTKIV
                     QIIRAQDPGRWIVVKPGPWGGSRGYGDFSPLPFPKLVYSVHIYAPIAYTHQGIRGHPA
                     GVSYPGKIGKHDWNKQLLERVIAPVVSFQQRYNVPVWVGEFSAVRWAPGSDQYLRDLV
                     SLFESHGWGWSYFSVGGWTGWDPRFSSSPDHAKMVGSSSQRWQTLEALFNTSGA"
     gene            complement(2946..3851)
                     /locus_tag="VFX38_08635"
     CDS             complement(2946..3851)
                     /locus_tag="VFX38_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEX5314953.1"
                     /translation="MAERGYIHVVAAEVLAHERHAAGQHVLRLAAPAIAAGARPGSFV
                     HIDCGPQWLLRRPMSLMSAAPANGEIELLFKEIGHGTVNLAALEPGDAVSLIGPIGNA
                     FTSLRERPRRLLLGGGVGIPPILYYAQTLVAGGESPPWVVTGSEVPFPFLTARADLPL
                     PGAPAAASDSISRLEALGVPNRLASLGKLPGCFHGFVTELAQLVIEALNPDERDELAL
                     YACGPTAMLAATAALARRYVLPCQVALEEYMACAVGGCAGCTVEVTTATGAAMKRVCV
                     DGPVFDAAAVFPAVSPVEPREPIFD"
     gene            complement(3844..>4288)
                     /gene="pyrB"
                     /locus_tag="VFX38_08640"
     CDS             complement(3844..>4288)
                     /gene="pyrB"
                     /locus_tag="VFX38_08640"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF012412.4"
                     /note="catalyzes the transfer of the carbamoyl moiety from
                     carbamoyl phosphate to L- aspartate in pyrimidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX5314954.1"
                     /translation="IAHSRVARSTLRALAILGVRELRMVGPEPFLPAAGELPAETSTE
                     LASGLAGADVVIALRIQRERMQAAEFPDPQEYRERYGITIERIERFAKPDALLLHPGP
                     VNWGLELDPELAAWPRSLIREQVRNGVAVRMALLARLAQALHGDG"
BASE COUNT          712 a         1379 c         1428 g          769 t
ORIGIN      
        1 gggggcgctg aagctcaagg aaatttccta cattcacgcc gaggcgtatc ccgccggcga
       61 actcaagcac gggccgctcg cactcgtcga cgaaagcatt ccggtggtcg ccgtcgcacc
      121 caacgacgag acgctcgcga agttgaaatc gaatctctcc gaagtgcgcg cccgcggcgg
      181 gaacctgatc gtgttcaccg accgcgaggc ccgcttcgcg ccgagtcccg atctcaccct
      241 ggtcgagctg tcgatcccgc aaggtctgct gctcgatccc gtcgtctata cgattccgct
      301 gcagcttctc gcctaccacg tcgcggtgct gcgggggacg gatgtcgatc agccgcgcaa
      361 tctggcgaag tccgtcacgg tcgagtgacg gcctcgcggt gtcccgcgtg ggagtgccga
      421 taatttataa tatccggttc ccggggttgc tcgaagcgtt gcgggccggc tcccgccccc
      481 gacgccaatc ctccgaacga agtaatcggc ctgtggtcca acacgagaat ccacgtatgt
      541 tgcgggttgc aatcatcgaa ccggtgggcg gacactgcgg aatggattac tacgacttcg
      601 ggctcgcgcg cggcctcgcc aatgccggcg cggacgtggt tctgcatacc tgcgacgaaa
      661 ccgagattcc gcgagatgct cccttcgagg tgcgcctgag ttatcgcggg atattcggcg
      721 aggcgcccaa atggcgccgc gcgctgcgct acatgcgtgg agcgctgggt gcccttgtct
      781 cggccgtggg cgagaggcgc cggatcgttc atttccatct ctttcacatc cgcttcgcgg
      841 aactcgcgaa catcgtcgtg gcccgccttc tcgggcgcaa ggtcgtaatc accgctcacg
      901 acgtcgagtc attcgtcggc tcgctcgaaa gccggcggat tcgcaagttc gtctacggcg
      961 ccgccgatcg cgtcattgcc cacaacgacg tgagccgcgt cgagctcatc gagaacgtcg
     1021 gcgtcgatga gcgcaagatc cgtcagatcc gccatggcaa tttcctcggg ctggtgcctg
     1081 aactgcccga ttccgcagcg gcgcgcagac gtgtgggcgt gtctcccgcc gcgcgcgtga
     1141 ttctctattt cggacagatc aaggaggtca agggcgtcga cgttctgctg cgcgccatgc
     1201 ccggcgtcgt ggcgcagttt ccggacgcgg tactgctcct cgccgggaaa tcctggaaga
     1261 cggacttttc ccgttatcag gccgtgatcg acgaactcgg aatcggcgct tcctgcgtgg
     1321 tgcaccatcg cttcattcct cccgaggaac tgccgtcctt tttttctgct gccgacgtga
     1381 tcgcgctacc ctaccgccgc atctatcaga gcggcgtcgt tcttttgtcc atgagttacg
     1441 gcaagccggt cgtggcgtcg gatcttcccg gctttacgca gttgctcgag gacggcgttt
     1501 ccggttatgt tttctcggac ggcgaccccg aatctctctg cgcgcgcctg tgtgcggcgc
     1561 tcgcggatcg ttccgagcgg gaggcggtcg gcagggccgc cctcgagaag gtgatcgagc
     1621 attacgattg ggatcattcc gggcgcgata ccgcccgcgt ttacgaggag attttatgat
     1681 tacgaatcgt tgcgcatcgt tcctaaaagt gacggcggcg gccggcgccg tttccctttt
     1741 ttcgttgctt gccgccggct gcatggcatc cggtgcgaat gcgacggcgc cgggcgactc
     1801 cggcaacgcg gttgcccccc gcgggaccgc ggagatcgcg gactcgacgc tccctcccca
     1861 ctggccctgg cgtggcgtga ccatggtcca cggggtcaag aacggcgcgc agcccgcgga
     1921 cgtggacgaa ttcgtcaagg ccttcggaat caacagcgtc catctctatc cgatcaccgg
     1981 gaagtatggc ccgcgggaag ggctcagcga tgccgcagcc tgggacgcct cgatgaaatg
     2041 gctggacgcg atgatcgcgg cctgtgagcg ggatcacgtc atggcgatcg tgcatctcac
     2101 gtcgtttccg aagccgggcg ggggttatta caacgagcat ctcgcgagtt tctggaacaa
     2161 cccctcggcc gtcaacgaaa tctacacccg cacggccgcg ctggtgaaac atctcggctc
     2221 ctggggcgac gacgtggtgg cgttcgacat catcagcgaa cccaccatga agacgagcct
     2281 cggcgtccag gcacctcgcg actggaccaa gatccagacg aaaatcgtcc agatcatccg
     2341 cgcccaggac ccgggtcgct ggatcgtcgt gaagccgggg ccctggggcg gttcgcgagg
     2401 ctatggggac ttctcgccgc ttccgtttcc aaagctcgtc tacagcgtgc acatctacgc
     2461 tcccatcgcc tacacgcatc agggaattcg cggccacccg gcgggcgtga gttatcccgg
     2521 aaagatcggc aagcacgatt ggaacaaaca actgctcgag cgggtgattg ctccggtcgt
     2581 ctccttccag cagcgctata acgtcccggt gtgggtgggc gaattcagcg cggtgcgctg
     2641 ggcgcccggc tccgatcagt acctgcgcga cctggtgagt ctcttcgaaa gccacggatg
     2701 gggatggtcg tatttcagtg tcggaggctg gaccggctgg gatccgcgct tcagttcgtc
     2761 ccctgaccac gcaaagatgg tcggaagcag ttcgcaacgc tggcagactc tcgaggcgct
     2821 gtttaacacc tccggcgcat gattggcgga agccggggca actcgggcga cggattccgg
     2881 aaagtccggc ggcaccgtcg cgaacggctc gcggagccgg cgggggcgct cgccggtgcg
     2941 gggcctcagt cgaagatcgg ttcgcgcggc tcgacgggcg acaccgcggg gaacacggcc
     3001 gcggcatcga agaccggccc gtcgacgcag acccgcttca tcgccgctcc tgtcgcggtc
     3061 gtgacctcga cggtgcaacc ggcgcagccg ccgaccgcgc aggccatgta ttcttccagc
     3121 gccacctggc aggggagaac gtagcggcgc gcgagcgcgg cggttgcggc gagcatcgcc
     3181 gtcgggccgc aggcatagag tgcgagttcg tcgcgttcgt cggggttaag cgcttcgatc
     3241 acgagttggg cgagttcggt cacgaatccg tggaagcagc cggggagctt gccgaggctc
     3301 gcgagtcgat tgggcacgcc gagcgcttcg aggcgcgaga tcgagtcgct cgccgcagcc
     3361 ggagcacccg gcaacggcag gtcagcgcgc gccgttagaa acgggaacgg cacttcggag
     3421 cccgtcacca cccagggcgg cgattcgccg ccggcaacga gcgtctgcgc gtagtagagg
     3481 atcggcggaa tgccgacgcc gccgccgagg agcaggcggc ggggccgctc gcgcaagctc
     3541 gtgaaggcgt tgccgatggg gccgatcagg ctgacggcat cgccgggttc gagtgcggcg
     3601 agattgacgg tgccgtggcc gatttccttg aagagcagct cgatttcgcc gttcgccgga
     3661 gcggcgctca tgagcgacat cgggcggcgc agcaaccatt gcgggccgca gtcgatatgt
     3721 acgaagcttc cggggcgagc gccggcggcg atggccggag ccgcgagccg caatacatgc
     3781 tgcccggcgg catgacgttc gtgcgcgagc acctctgcgg cgacgacgtg gatatagccg
     3841 cgctcagcca tcgccgtgca gggcctgggc aagcctggcg aggagcgcca tgcgcacggc
     3901 tactccgttt cgcacctgct cgcgaatgag gctgcgcggc caggccgcga gttcgggatc
     3961 gagttcgagc ccccaattga ccggcccggg atgcagcagc agcgcgtccg gtttggcgaa
     4021 gcgttcgatc cgctcgatcg tgattccata acgttcgcgg tactcctgcg gatccgggaa
     4081 ttccgccgcc tgcattcgct cgcgctggat gcggagcgca atcacgacgt cggcgcccgc
     4141 aaggccggac gcgagttccg tgctggtttc ggccggcagc tctccggcgg ccggcagaaa
     4201 aggctcgggc ccgaccatcc gcagttcgcg taccccgagg atcgcgagcg cccgcaaggt
     4261 cgagcgcgcg acccgcgaat gcgcgata
//
LOCUS       DASUJG010000407         3975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_362979, whole genome shotgun sequence.
ACCESSION   DASUJG010000407 DASUJG010000000
VERSION     DASUJG010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362979"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..1639)
                     /gene="lpdA"
                     /locus_tag="VFX38_08645"
     CDS             complement(206..1639)
                     /gene="lpdA"
                     /locus_tag="VFX38_08645"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX5314955.1"
                     /translation="MSDDNYDVIVIGGGPGGYVAAIRAAQNGLKAACIDNWQDRENKH
                     AFGGTCLNAGCIPSKAMLEASELYERTGHEFGDFGIKVEKYALDLATMQKRRAAIVKQ
                     HTGGIAQLFQGNKVTGLAGTGKLGKGGKVSFEGFDGKKQTLEAKNVILAAGSVPVELK
                     SLPFDHETIVDSWDALEFDRVPKRLGVIGAGVIGLEMGSIWRRLGSEVVILEALEEFL
                     PMVDEQVAKEAQKQFKKQGLDIRLGTKVTGAKKSKSGVTLSYETKDGKQTLEVDKVLV
                     AVGRRPFAEGLLEKDAGVAVDERGFIKVDEECRTNVKGVWAIGDVVRGPMLAHKASEE
                     GVMAADLIAGKVAEVNYEAVPSVIYTAPEVAWVGKSAQQLKKDGVPVKTGRFPFVANG
                     RANALGMPAGMVKMIAHQQTDEILGVHIVGPYASELIAEAVLAMEYRASAEDIQRTMH
                     AHPTLSEAMHEAALAVDKRAIHVVNRR"
     gene            complement(1748..3061)
                     /gene="odhB"
                     /locus_tag="VFX38_08650"
     CDS             complement(1748..3061)
                     /gene="odhB"
                     /locus_tag="VFX38_08650"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEX5314956.1"
                     /translation="MSTEIKVPELPESITEATLVAWHKKPGEAVARDENLVDLETDKV
                     VLEVPAPADGVLKEIKVEDGATVTAGQLLAVLDEGAEAGAGSDAQKEEKKPPKEEAGA
                     DDKSESGGSQSKPEAATEEAEEEPETSAQSGGSTKPLSPSVRRLVEEHELDPARIPAS
                     GRDGRLTKSDVLGFLERDDDAKKEPAPKNAEAAKPAPARRPAAGAREQRRVPMSRIRA
                     RIAERMLEAQATTAMLTSFNEVDMSAVMDIRKRYREDFEKKHGVRLGYLSFFVRAAVH
                     ALQRYPVVNASVEDGDIVYHDYWDIGVAVSTERGLVVPVIRDADALSSAEIEAQVIDY
                     ANKAREGGLTLEDITGGTFTITNGGVFGSLISTPIVNPPQSAIFGMHKIEERPVAIAG
                     EVKIRPMMYIALTYDHRIVDGRDAVLFLVAVKEALEDPARILIDL"
     gene            complement(3058..>3975)
                     /gene="sucA"
                     /locus_tag="VFX38_08655"
     CDS             complement(3058..>3975)
                     /gene="sucA"
                     /locus_tag="VFX38_08655"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672944.1"
                     /note="SucA; E1 component of the oxoglutarate
                     dehydrogenase complex which catalyzes the formation of
                     succinyl-CoA from 2-oxoglutarate; SucA catalyzes the
                     reaction of 2-oxoglutarate with dihydrolipoamide
                     succinyltransferase-lipoate to form dihydrolipoamide
                     succinyltransferase-succinyldihydrolipoate and carbon
                     dioxide; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX5314957.1"
                     /translation="HLAEDQAPFMVYDSLLSEAAVVGFEYGYATARPKTLVLWEAQYG
                     DFANNAQVMIDQFISSGETKWGRLCGLVLLLPHGYEGAGPEHSSARLERYLQLCAGSN
                     MQVCIPTTAAQIFHLLRRQMLRRFRAPLVVMAPKSLLRHKLAASALEELAGGGFRLVV
                     PETEQLDPKRVRRVVFASGKVYYELLARRGKEKRNDVALVRIEQLYPFPREAYAAEIN
                     LYRDAREIVWCQEEPENQGAWYQIQHRLREPLTRRQTLIYAGREASAAPATGYARQHR
                     AEEDALVEAALGIGGRGEVQQKIARMESQ"
BASE COUNT          609 a         1335 c         1246 g          785 t
ORIGIN      
        1 ggccatattg attctcgata cgcctgccgc gcccctgtag cccgcgcagc tcggaccggg
       61 tctcccttcg tgacgcttgt cgcagcagta atcgtaggcc cgcataagcg aagcgttgcg
      121 ggcaattcga cggttgccgg caacaccctt cgggcttatg ccggcctacg gggccaagtt
      181 cgagacgaac gaaaattcgg gtaaatcagc ggcggttgac gacgtggatg gcgcgcttgt
      241 ccaccgcgag cgcggcttcg tgcatcgcct cggagagagt cggatgcgcg tgcatcgtgc
      301 gctggatgtc ttcggcgctt gcgcggtatt ccatcgcgag cacggcttcc gcgatcaact
      361 ccgaggcata cgggccgacg atgtgcacgc cgagaatctc gtcggtctgt tgatgcgcga
      421 tcatcttcac catgccggcc ggcatgccta atgcgttggc gcggccgttg gcgacgaagg
      481 gaaaacggcc cgtcttcact ggcacgccgt ccttcttcaa ctgctgtgcg ctcttgccga
      541 cccaggccac ctccggcgcc gtgtaaatca ccgagggcac ggcttcgtag ttgacttctg
      601 cgaccttgcc ggcgatgaga tccgcggcca tgacgccctc ctcgctcgcc ttgtgcgcga
      661 gcatcgggcc gcgcacgaca tcaccgatcg cccacacgcc cttgacgttg gtgcggcatt
      721 cctcgtccac cttgatgaat ccgcgctcgt ccacggcgac gcccgcgtcc ttctccagca
      781 ggccttcggc aaacgggcgg cgcccgactg cgaccagcac cttgtccact tcgagcgtct
      841 gcttgccgtc cttcgtttcg taggaaagcg tgacgccgct cttgcttttc ttcgcgcccg
      901 tgaccttggt gccgagccgg atgtcgaggc cctgcttctt gaactgcttc tgcgcttcct
      961 tcgcgacctg ctcgtccacc atcggcagga attcctcgag tgcttcgagg atcacgacct
     1021 cgctgccgag ccggcgccag atgctgccca tctcgagccc gatgacaccg gcgccgatca
     1081 cgccgaggcg tttgggcacg cggtcgaatt cgagcgcgtc ccaggaatcc acgatcgtct
     1141 cgtgatcgaa ggggagcgac ttcagttcga ccggcaccga acccgcggcg agaatcacgt
     1201 tcttcgcttc gagcgtctgc ttcttgccgt cgaagccctc gaagctcacc ttgccgccct
     1261 tgccgagttt cccggtgccg gcgagtcccg tcaccttgtt gccctggaag agctgcgcga
     1321 tgccgccggt atgctgcttg acgatcgcgg cgcggcgctt ctgcatggtc gcgagatcga
     1381 gcgcgtactt ctcgaccttg atgccgaagt cgccgaattc gtgcccggtg cgctcgtaca
     1441 gctccgaggc ttcgagcatc gccttggacg gaatgcagcc cgcgttgagg caggtgccgc
     1501 cgaaggcatg cttgttctcg cgatcctgcc agttgtcgat gcaggcggcc ttcagcccgt
     1561 tctgtgccgc gcggatcgcg gcgacgtagc cgcccggtcc gccgccgatc acgatcacgt
     1621 catagttgtc gtcactcatc tttctctcca gagtaggccg gcacaaggcc gcgaggccgt
     1681 tgccggcatc gaacgtcaat cgccgggaac gcgcgcaagg cgcgcttatc ccggcctaca
     1741 acaattctca aaggtcaatg aggatccgcg cggggtcttc gagcgcttcc ttgacggcga
     1801 cgaggaagag cacggcgtca cgcccgtcca cgatccgatg atcgtaggtg agcgcgatgt
     1861 acatcatcgg gcggatcttc acctcgccgg cgatcgcgac ggggcgctcc tcgatcttgt
     1921 gcatgccgaa aatcgcgctc tgcggcgggt tcacgatcgg cgtcgaaatc agcgagccga
     1981 agacgccgcc gttggtgatg gtgaaggtgc cgccggtaat gtcctcgagc gtgagtccgc
     2041 cctcgcgcgc cttgttcgcg tagtcgatga cctgcgcctc gatctcggcg gaggaaagcg
     2101 cgtccgcatc gcggatcacg ggcacgacga gcccgcgctc ggtcgacacc gcgactccga
     2161 tgtcccagta atcgtgatag acgatgtcgc catcctcgac cgaggcgttg acgaccgggt
     2221 aacgctgcag ggcatgcacc gcggcgcgca cgaagaacga gagatagccg agacggactc
     2281 cgtgcttctt ctcgaagtcc tcgcggtagc gcttgcggat gtccatgacg gcgctcatgt
     2341 ccacttcgtt gaaggaagtg agcatggcgg tcgtcgcctg cgcctcgagc atgcgctcgg
     2401 cgatgcgcgc gcggatgcgc gacatcggca cgcgcctctg ctcgcgcgcg ccggccgcgg
     2461 gacgccgcgc gggtgcgggc ttcgcggctt cggcgttttt cggcgccggt tccttcttcg
     2521 cgtcgtcgtc ccgctccaga aacccgagaa cgtcggattt ggtcaggcgc ccgtcgcgtc
     2581 cggaagcggg aatgcgcgcg ggatcgagtt cgtgctcctc gacgaggcgg cgcaccgaag
     2641 gagaaagagg cttcgtgctg ccgccgcttt gcgcagaggt ttccggctct tcttcggctt
     2701 cctcggtggc ggcttcgggc ttcgattgcg agccgccgga ttccgatttg tcatccgcgc
     2761 cggcctcttc cttcggcggt ttcttctctt ctttctgtgc atcgctcccg gcgcccgctt
     2821 cggctccttc gtccagtacc gcgagaagct gaccggcggt gacggtcgcg ccgtcctcga
     2881 ccttgatttc cttcagcacg ccgtcggcgg gcgccggcac ttcgagtacg accttgtcgg
     2941 tttcgagatc caccagattc tcgtcgcggg caacggcctc tcccggcttc ttgtgccagg
     3001 cgacgagcgt cgcctcggtg atggattcgg gcagttcggg aaccttgatt tcggtgctca
     3061 ttgggactcc attcttgcga tcttctgttg cacttcgccg cgcccgccga tgccgagcgc
     3121 cgcctccacg agtgcatcct cttccgcgcg atgctggcgc gcgtagccgg tcgccggcgc
     3181 ggccgaggcc tcgcgcccgg cgtagatgag cgtctggcgc cgcgtcagcg gttcgcgcag
     3241 acgatgctga atctgatacc aggctccctg gttttcgggt tcctcctgac accacacgat
     3301 ctcgcgcgca tcgcgataga gattgatctc ggccgcgtag gcttcacgag gaaacggata
     3361 gagctgctcg atgcgaacga gtgcgacgtc gttccgcttt tccttgccgc ggcgcgcgag
     3421 cagttcgtaa tagaccttgc cgctcgcgaa gacgacgcgg cgcacccgct tgggatcgag
     3481 ctgctcggtc tcgggaacga cgagtcggaa gccgccgccc gccaattcct cgagcgcgga
     3541 ggccgcgagc ttgtggcgca acagactttt cggcgccatc accaccagcg gcgcgcggaa
     3601 gcggcgcagc atctggcggc gcagcagatg aaagatctgc gccgccgtcg tcggaatgca
     3661 gacctgcatg ttgcttccgg cacaaagctg gaggtagcgt tcgagccgcg ccgaggaatg
     3721 ctcgggaccg gcgccctcgt acccgtgcgg aagcagcagc accagcccgc agagccgccc
     3781 ccacttcgtc tcgcccgaac tgatgaactg atcgatcatc acctgcgcgt tgttcgcgaa
     3841 gtcgccgtat tgtgcctccc acaacaccag cgtcttcggc cgcgccgtcg catagccgta
     3901 ttcgaatccg acgaccgcgg cttcggacag gagcgagtcg tacaccatga acggcgcctg
     3961 atcctccgcg agatg
//
LOCUS       DASUJG010000408         5907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_606072, whole genome shotgun sequence.
ACCESSION   DASUJG010000408 DASUJG010000000
VERSION     DASUJG010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5907
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606072"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(239..1156)
                     /gene="fmt"
                     /locus_tag="VFX38_08660"
     CDS             complement(239..1156)
                     /gene="fmt"
                     /locus_tag="VFX38_08660"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX5314958.1"
                     /translation="MRIVFAGSGDFAVPILDSLIGSAQEIALVVTQAARPAGRGRRLS
                     ATPVALAAARMNLPVETPRSLADCEPSLRALAPDVLVVVDYGRIVPAAVLAAARHGGL
                     NVHPSLLPRWRGAAPVARAIEAGDRDTGVAIMQMDAGLDTGAILALERSAIGAEETAG
                     ELEARLAQRGAALLLATLDTLGTSATRATAQRGEASYAAKVLVREARLDFSEPAERLA
                     RRVRAFNPRPGAWAECAGERLRLLRARPRAGSLAAAPGTIVATQATGLAIATGEGLLE
                     VTELQRPGRRIQSAATLCGDERWIGRVLC"
     gene            complement(1173..1703)
                     /gene="def"
                     /locus_tag="VFX38_08665"
     CDS             complement(1173..1703)
                     /gene="def"
                     /locus_tag="VFX38_08665"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX5314959.1"
                     /translation="MALLPILHFPDPRLRERALDVESFDAALEATIADMFETMYAAPG
                     IGLAATQLGIARRIAVIDVSEDKSEPRVLVNPRLIESSGRRESEEGCLSVPGYYDTIE
                     RAERLRVRALGRDGEPFELEAEDLLAVCIQHELDHLNGQLFIDHLSDLKRRRLRRQIE
                     KARREARAEETGPAAG"
     gene            1843..3003
                     /locus_tag="VFX38_08670"
     CDS             1843..3003
                     /locus_tag="VFX38_08670"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEX5314960.1"
                     /translation="MRSWSSFAFPVTVALVAALGLAACANQPCAPQVAQPLAKTPPAT
                     AAPVPPLAKALQAHPPFRYVVQPGDTLWGISSRFLRSPWLWAEIWYENPYIHNPHLIY
                     PGDVITLTYPNGEAALSISRGGTMVATTSAELGTRFVAPKVRVTPLAHAIPTIPYDEI
                     APLLSKPRVMGAEAYASAPYVLRPLEDRLLAASPGSIYARGLDGTGDDPGSAWAVVEK
                     TKVLKEPPSGCSLGYEVAYLGRAEVRRAGDPTSLQLVQSTQEIKPGDRLVPVETGVVP
                     SQFPLLAPNVVVDADIIDVIGGLEEVGQYQVVVLDRGSQSGLRMGDVLAIYRRGEWMK
                     DPYAHGNLSGSVLLPNERSGHLVVFKVFPQVSFALVMSAQRPIRINDFATNP"
     gene            3011..4138
                     /gene="dprA"
                     /locus_tag="VFX38_08675"
     CDS             3011..4138
                     /gene="dprA"
                     /locus_tag="VFX38_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX5314961.1"
                     /translation="MDRGADDAALRVRTLAALLRAPGVGEARLRRLLDEFGSPLAIAR
                     AGRARLRAAGLPESALRASASSAAAEDLQWASAPGHHLVAWGEAAYPPLLAAIPDPPP
                     LLFVVGDPAVLADPQIAIVGSRNPSADGRDIAREFAAFLAGAGFTVTSGLALGIDAAA
                     HAGALDADGATIAVMATGPEQVYPRRHEELAQRIAAKGALTSEFPPGTPPLPEHFPRR
                     NRIISGLASATVVIEAALGSGSLITARLAAEQGREVFAVPGSIHSPLARGCHALIREG
                     ATLVERPSDILESLGAFAPASAAEEPREPDSDANADDPDYRAMLDALGFHPVSADTLG
                     ERTGLTPQAISSMLLILELRGEIEALPGARYARRRRGQEPK"
     gene            4135..4596
                     /locus_tag="VFX38_08680"
     CDS             4135..4596
                     /locus_tag="VFX38_08680"
                     /inference="COORDINATES: protein motif:HMM:NF016264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HEX5314962.1"
                     /translation="MNATVLDVLLYLFENCLQDEEPPPRAALERELRSSGFPLARVHQ
                     ALDWLEALVEDVPAPAGRTDALRVFSDFECDRLSAEARGFLMHLERCGILEPEQREIV
                     IAHLLELDEEPAGIEETKWVTLITLFHQHGQEAAFSRMQDLLHADFATATH"
     gene            4639..>5907
                     /gene="topA"
                     /locus_tag="VFX38_08685"
     CDS             4639..>5907
                     /gene="topA"
                     /locus_tag="VFX38_08685"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX5314963.1"
                     /translation="MSHSVVVVESPAKAKTLKKYLGADADVYASYGHVRDLVPKEGAV
                     APDSGFAMRYEPIERNERHVAAIARAVKKADRLVLATDPDREGEAISWHLRELLEERG
                     ALDGKEVVRVAFHEITERAIREAIAHPRELAGDLINAQQARRALDYLVGFNLSPLLWK
                     KIKRGLSAGRVQSPALRLIVEREEEIEAFAAQEFWRIEAALAREEQKFSARLYEYRGE
                     KVQQFSFTEAKTAQAARAEIARAAAGNLVVKAIERKERRRNPAPPFITSTLQQEAARK
                     LGFTTRRTMRIAQQLYEGVDLGEGPVGLISYMRTDSTQLASEAVAEIRDYIAGRFGKE
                     NLPPKARVYRTRSKNAQEAHEAIRPTVVGREPRAIAAKLSVEQARLYELIWNRTVACQ
                     MIHATLDLVAVDLAATDVAVFRATGSTIRHP"
BASE COUNT          931 a         2104 c         1972 g          900 t
ORIGIN      
        1 caggctgctc gcggcatcga gaacccggaa gcccggcgcc gcatcggccg gccgagcgga
       61 gccgagaagc gcgccgagca cgaggacggc gagaagaacc tggcgcgccg ggcgcgtcgc
      121 cttcgccggc gcgtcgcccg ccctgctgcg cggatgtccg ctcacccgcg gccgctcgcg
      181 ccgaccggaa acagcgtcat tgccgcgatg ataccgggat tcgcggcagc gcgagcgctc
      241 agcacagcac tcgcccgatc cagcgctcgt cgccgcagag cgtcgccgcc gactgaatcc
      301 gccggcccgg gcgctgcaat tccgtgactt cgagcagacc ctcgccggtc gcgattgcga
      361 gaccggtcgc ctgtgtcgcc acgatcgttc cgggcgccgc cgccaaactt cccgcccgcg
      421 gcctcgcgcg gagcaggcgc aggcgctcgc ccgcgcactc cgcccaggcg ccgggacgcg
      481 gattgaaggc gcgcacgcgg cgggcgagcc gctcggccgg ttccgagaag tcgagccgcg
      541 cctcgcggac gagtactttc gccgcgtaac tcgcctcgcc gcgctgcgcc gtcgcgcgcg
      601 tcgccgaagt cccgagcgtg tcgagcgtcg cgagcagcag cgccgcgccg cgctgcgcga
      661 ggcgcgcctc gagttccccc gccgtttcct ccgcgccgat cgcgctgcgc tcgagcgcga
      721 ggatcgctcc ggtgtcgagg ccggcatcca tctgcatgat cgcgacgccc gtgtcgcgat
      781 cgcccgcttc gatggcccgc gcgacgggtg ccgcgccgcg ccagcgcggc aagagcgaag
      841 gatggacatt gagtccgccg tgacgcgcgg ccgcaagaac ggcggcggga acgatgcggc
      901 cgtagtcgac gaccacgagc acatcgggtg cgagcgcccg caggctcggc tcgcagtcgg
      961 caagcgatcg tggagtctcg acgggcaggt tcatccgcgc ggccgcaagt gccaccggcg
     1021 tcgcgctcaa gcggcggccg cggccggccg gcctcgccgc ctgcgtcacg accagtgcga
     1081 tttcctgcgc cgagccgatc agggagtcga ggatgggtac ggcgaaatcg ccgctgccgg
     1141 caaatacgat ccgcatggcc gacgctcgcg tctcagcccg ccgcggggcc cgtctcctcc
     1201 gcccgagcct cccgccgcgc cttctcgatc tggcggcgca ggcgtcgacg cttgagatcc
     1261 gagagatggt cgatgaagag ctgaccgttg agatgatcga gttcatgctg gatgcagacc
     1321 gcgagcagat cctcggcctc gagttcgaag ggctcgccgt cacgcccgag cgcccgcacc
     1381 cgcaggcgct cggcccgctc gatcgtgtcg taataaccgg gaaccgaaag acagccctcc
     1441 tcgctctcgc gccggccgct gctttcgatg agtcgcggat tgacgaggac gcgcggttcg
     1501 ctcttgtcct cggatacgtc gatgacggcg atgcgccggg cgattccgag ctgcgtcgcc
     1561 gcaagcccga tgccgggggc ggcatacatc gtctcgaaca tgtcggcgat cgtcgcctcg
     1621 agcgcggcat cgaagctctc gacgtcgagc gcccgctcgc gcaggcgcgg atcggggaaa
     1681 tgcaaaatcg gaagcaaggc catcgtccgg tttctcgcgg caaaattttc tgctacagtt
     1741 ggcgccgatt atagcgaacg gtctcgcccc gcccgggcgg acaaacggcc gggaacgcgc
     1801 tccggggagg agttggaact gtccgcgctg ggagccgatg ccatgagaag ctggtcctca
     1861 ttcgcctttc ccgtcacggt ggcgctggtc gcggcgctcg ggctcgccgc ctgcgccaat
     1921 cagccctgcg caccgcaggt cgcgcagcct ctcgcgaaga cgccgccggc caccgccgcc
     1981 ccggtgccgc cgcttgcaaa ggcgctgcag gcgcaccctc cgtttcgcta cgtggtgcag
     2041 ccgggcgaca cgctctgggg aatctcgtcg cgctttctgc gcagtccctg gttgtgggcc
     2101 gaaatctggt acgaaaatcc ctacattcac aacccgcatc tcatctatcc cggcgacgtc
     2161 atcacgctca cctacccgaa cggcgaggcg gcgctctcga tttcgcgcgg cggcacgatg
     2221 gtcgcgacca cctcggccga gctcggcact cgtttcgtcg ccccgaaggt tcgggtcacg
     2281 ccgctcgcgc atgcgattcc gacgattccc tatgacgaga tcgcgccgct gctgtccaag
     2341 ccgcgggtga tgggcgccga ggcctacgcc agcgcaccgt acgtgctgcg gccgctggag
     2401 gaccggctgc tcgccgcgag cccgggctcg atctatgcgc gcggcctcga cgggacgggc
     2461 gacgatccgg gcagcgcctg ggccgtggtc gaaaagacca aggtcctgaa ggagcctccg
     2521 agcggctgct cgctcggcta cgaggtcgcc tatctcggcc gcgccgaagt gcggcgtgcc
     2581 ggcgatccga cgagcctcca actggtgcag agcacccagg agatcaagcc cggcgaccgt
     2641 ctcgtgccgg tggaaaccgg agtcgtgccc tcgcagtttc cgctgcttgc gccgaacgtc
     2701 gtcgtcgatg ccgacatcat cgacgtcatc ggtggcctcg aggaggtcgg ccagtatcag
     2761 gtggtcgtgc tcgatcgcgg cagccagagc gggctgcgca tgggggacgt gctcgcgatc
     2821 taccgccgcg gcgaatggat gaaggatccc tacgcccacg gcaatctctc ggggagcgtg
     2881 ctgctcccga acgagcgcag cggtcacctc gtcgtcttca aggtcttccc ccaggtttcc
     2941 tttgctctcg tgatgtcggc gcagcgaccg atccgcatca acgacttcgc gaccaatccc
     3001 tgaggcggag atggatcggg gcgccgacga cgccgcgctt cgcgtccgaa cactcgcggc
     3061 actgctgcgc gctcccggcg tcggagaggc gaggcttcgc cgcctgctcg atgaattcgg
     3121 ctcgccgctc gcgatcgccc gcgccggcag agcccgcctg cgcgccgcgg ggctgccgga
     3181 gtcggcgctt cgcgcaagcg cgtcctccgc cgcggcggaa gatctccaat gggcgagcgc
     3241 gcccggtcat catctcgtcg cctggggcga agcggcctat ccgccgctgc tggcggcgat
     3301 tcccgatccg ccgccgctgc tcttcgtggt cggcgatccg gccgttctcg cagacccgca
     3361 gatcgcgatc gtgggctcgc gcaacccgag cgccgacggg cgcgacatcg cccgcgagtt
     3421 cgcggccttt cttgcgggcg ccggattcac cgtcaccagc ggactcgcgc tcggcatcga
     3481 tgccgcggcc cacgccgggg cgctcgatgc cgacggcgcg acgatcgccg tcatggctac
     3541 cggaccggag caggtgtatc cgcgacgtca cgaggaactc gcgcaacgca tcgcggcaaa
     3601 gggcgcgctc acgagcgagt ttccgcccgg tacgccgcct ctgcccgaac acttcccgcg
     3661 ccgcaaccgc atcatcagcg gtctcgcctc ggcgacggtc gtgatcgaag cggcgctcgg
     3721 ctccggctcg ctcatcacgg cgcggctcgc cgccgagcag ggacgcgagg tcttcgccgt
     3781 tccgggctcg attcactcgc cgctcgcccg cggttgccat gccctcatcc gcgagggcgc
     3841 gaccctcgtc gagcgtccct ctgatattct tgagtcgctg ggcgcgtttg cgccggcgtc
     3901 ggccgcggag gaacctcgcg agcccgattc ggatgcaaac gcggacgacc cggactatcg
     3961 ggcgatgctc gatgcgctgg gttttcaccc cgtgagtgcc gatacgctcg gcgagcgtac
     4021 gggattgacg ccccaggcga tatcctccat gcttctgatc ctcgaactgc ggggcgagat
     4081 cgaagcgctg cccggtgcgc gctacgcgcg gcgacgacga ggccaggaac cgaaatgaat
     4141 gcgaccgtac tcgatgtgct cttgtatctg ttcgaaaact gcctgcagga cgaggagccg
     4201 ccgccgcgcg cggcgctcga acgggaactt cgcagcagcg gatttccgct cgcccgggtg
     4261 catcaggcgc tcgactggct cgaggctctc gtcgaggacg tgccggcgcc ggccggcaga
     4321 accgacgcct tgcgcgtgtt ctccgacttc gaatgcgacc ggctgagcgc cgaagcgcgc
     4381 ggctttctga tgcatctgga gcgctgcggc attctcgagc ccgagcaacg cgagatcgtg
     4441 atcgcgcatc tgctcgaact cgacgaggag ccggccggca tcgaagagac gaaatgggtg
     4501 acgctgatca cgctctttca ccagcacggt caggaggccg ccttctcccg catgcaggat
     4561 ctgctccatg ccgacttcgc gacggccacg cattgaagcg ggccggccgc gagtccgcgc
     4621 gttgaaccgg acggccgcgt gagtcattcg gtcgtcgtcg tcgagtcgcc cgccaaggcg
     4681 aaaacgctca agaaatatct cggagcggac gccgatgtgt atgcctccta cggtcacgtg
     4741 cgcgacctcg tgcccaagga aggcgccgtc gcgccggact cgggcttcgc gatgcgctac
     4801 gaacccatcg agaggaacga gcgccatgtt gccgccatcg cgagggcggt gaagaaggcc
     4861 gaccggctcg tgctcgcgac cgatcccgac cgcgagggcg aggcgatttc ctggcatctt
     4921 cgcgagcttc tcgaggaacg cggcgcgctc gacggcaagg aagtggtgcg cgtcgccttt
     4981 cacgaaatca cggagcgcgc gatccgcgag gccatcgctc atccgcgcga actcgcgggc
     5041 gatctcatca acgcacagca ggcaaggcgc gcactcgatt atctcgtcgg cttcaatctt
     5101 tcgccgctgc tgtggaaaaa aatcaagcgt ggactttccg ccgggcgcgt ccagagtccg
     5161 gcgctgcgac tcatcgtcga gcgcgaggag gaaatcgagg ccttcgcggc gcaggagttc
     5221 tggcgcatcg aggccgcgct cgcgcgcgag gagcagaagt tcagcgcgcg cctctacgag
     5281 taccgcggcg aaaaggtcca gcagttttcc ttcacggaag caaaaaccgc ccaggccgcg
     5341 cgcgccgaga tcgcgcgcgc tgcggccgga aacctcgtgg tcaaggccat cgaacgcaag
     5401 gagcggcggc gcaatcccgc acctcccttc atcacctcga cgctgcaaca ggaagcggcg
     5461 cgcaaactcg gcttcaccac ccggcgcacg atgcgcatcg cgcagcaact ctacgaaggc
     5521 gtcgacctcg gcgaaggtcc ggtcggactc atcagctaca tgcgcacgga ctccacgcag
     5581 ctcgcgtccg aagcggtcgc ggaaatccgc gactacatcg ccgggcgctt cggcaaggaa
     5641 aatcttccgc cgaaggcgcg ggtctatcgc acccgcagca agaacgcgca ggaggcgcac
     5701 gaggcgatcc gcccgaccgt cgtcgggcgc gagccgcgcg cgatcgcggc gaagctcagc
     5761 gtcgaacagg cgcggcttta cgaactcatc tggaaccgca ccgtcgcctg ccagatgatc
     5821 cacgcgacac tcgatctcgt cgccgtcgat ctcgcggcca cggatgtcgc cgtctttcgt
     5881 gcgaccggtt ccacgatccg ccatccc
//
LOCUS       DASUJG010000409         2394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1097077, whole genome shotgun sequence.
ACCESSION   DASUJG010000409 DASUJG010000000
VERSION     DASUJG010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097077"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1123
                     /locus_tag="VFX38_08690"
     CDS             <1..1123
                     /locus_tag="VFX38_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEX5314964.1"
                     /translation="SLRFFGGVGKLRARMRSGLDALGLTASLAFAPTPRAALWLARAD
                     ARTTPQRAEDLPGALGVLPLAVLNPEAQIARKFLRLGVKTLAQAFRLPREGLARRFGP
                     ELIHDWDRALGWSPEARRRYHAAPHFAIERELPGACDAAGELLPVLDELLGALVLALR
                     ERDAGIASFALIFRHDDLPDTRIVLRLFTPSRERARLERLLRTRLETLVLAAPVVAVG
                     IASGVFLPIVNPKTELLPDSREEAQAIDPLIEILHARLGGEAVFGLAFAADPRPERAW
                     RKSRPGAKSASPPAAAGPRPLWLFARPRPLPAREGIPWHANRPLALASGPERIEAGWW
                     EGGDVGRDYYLARSDAGARLWIYRDHRDGGAWFLHGLFS"
     gene            1120..>2394
                     /locus_tag="VFX38_08695"
     CDS             1120..>2394
                     /locus_tag="VFX38_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX5314965.1"
                     /translation="MSAPPAYAELHALSNFSFLRGASHPAELIARAAEHGYAALALTD
                     ECSLAGIVRAHVAAKETGLHLIVGSELRLEDGTRLVLLAENREGYGALAALITRARRR
                     VEKGSYRLRREDFDSPPAHCLALWLPDASRPERWRAEAEWLRERFSARAWIAVELLLS
                     GRDAEQLEGLRDIGRALSLPLAAAGDVHMHRRDRRMLQDALTAIRLKRPLREIRGELH
                     PNGERHLRSRARLARLYPPELLAETIRIAGRCRFGLEELRYEYPREIVPQGIAPAAHL
                     RALVEDGAARRWPAGIPAAARTQLEHELALIAELAYEPYFLTVHDIVRFARDRGILCQ
                     GRGSAANSAVCYCLGITELDPARSNLLFERFVSKERAEPPDIDVDFEHERREEVIQYI
                     YGKYGRERAALAATLITYRTRSAVRDLGKADRA"
BASE COUNT          348 a          813 c          813 g          420 t
ORIGIN      
        1 gagcctgcga tttttcggcg gcgtcgggaa attgcgggcg cgcatgcgtt ccggtctcga
       61 tgcgctcggt ctcacggcga gtctcgcgtt cgcgccgaca ccgcgggccg ccctgtggct
      121 ggcgcgggcg gatgcgcgca cgactccgca gcgggcggaa gatttgcccg gcgcgctcgg
      181 cgtcttgccg ctcgcggtgc tgaatccgga agcgcaaatc gcgcgcaagt ttctgcgtct
      241 cggagtgaag acgctcgccc aggccttccg gcttccgcgc gaagggcttg cgcgccgctt
      301 cggtcccgaa ttgatccacg attgggatcg cgcgctcggg tggtctcccg aagcgcggcg
      361 gcgctaccac gccgcgccgc attttgcgat cgagcgggaa ctgccgggag cctgcgatgc
      421 ggccggcgaa ctgctgccgg ttctcgacga actgctcggc gcgctggtat tggctcttcg
      481 cgaacgcgac gccggcatcg cgagcttcgc actgattttt cgtcacgacg accttccgga
      541 cacgcgcatc gtcctgcgtc tgttcacgcc cagccgcgag cgcgcacgtc tcgaacgcct
      601 gcttcgcacc cggttggaaa ccctcgttct cgccgcgccg gtcgtcgcgg tcgggatcgc
      661 gagcggcgtc ttcctgccga tcgtcaaccc caaaacggaa ctgcttcctg attcgagaga
      721 agaagcacaa gcaattgatc cgttgattga aatcctgcat gcacggctcg gcggcgaggc
      781 agtcttcggc ctcgccttcg ccgccgaccc ccggcccgaa cgggcctgga gaaagagccg
      841 gccgggagcg aaaagcgcgt cgccgcccgc agccgcgggt ccgcgtccgc tgtggttgtt
      901 cgcccgtccc cggccgcttc ccgcgcgcga gggaatccct tggcatgcga accggccgct
      961 ggcgctcgcg agcggtccgg aacggatcga agccggttgg tgggagggag gcgacgtcgg
     1021 ccgggattat taccttgcgc gcagcgatgc gggcgcgcgc ctgtggattt accgcgatca
     1081 tcgcgacggc ggtgcctggt ttctgcacgg cttgttttcg tgagtgcgcc gcccgcgtat
     1141 gcggaacttc acgcgctcag caacttcagc tttctgcgcg gagcttcgca tcccgcggaa
     1201 ttgatcgcgc gcgcggcgga acacggctat gcggcgctcg cgctgaccga cgagtgttcg
     1261 cttgccggga tcgtgcgcgc gcatgtggcg gcgaaggaaa ccggcctgca cttgatcgtc
     1321 ggcagcgaac tgcgcctcga agacggaacg cggctggtgc tgctcgcgga aaaccgtgaa
     1381 ggttatgggg cgctcgccgc actcatcacc cgcgcgcggc gacgtgtcga aaagggaagc
     1441 taccgtttgc ggcgcgagga tttcgattcg ccgccggcgc attgtctcgc gctgtggctc
     1501 cccgatgcct cgcgtccgga gcgttggcgt gccgaggccg agtggctgcg ggaacgcttc
     1561 tccgcccgcg cctggatcgc ggtcgaattg ctgctttcgg ggcgcgacgc cgaacaactc
     1621 gaaggcttgc gtgatatcgg ccgcgcgctg tccctgccgc tcgccgcggc cggcgacgtg
     1681 cacatgcacc ggcgcgatcg gcggatgctg caggatgcac tcaccgcgat ccggctcaag
     1741 cgtccgctgc gcgaaatccg cggcgaactt catccgaacg gcgaacggca tttgcgttcg
     1801 cgcgcgcggc tcgcgcgact ttatccgccc gagttgctgg cggagacgat ccgcatcgcc
     1861 gggcgttgcc gcttcgggct cgaggaactg cgctacgaat atccgcgcga gatcgtgccg
     1921 caggggatcg cgccggcggc gcatctgcgc gctctcgtcg aggacggcgc cgcccgccgc
     1981 tggcccgcgg gcattcccgc cgcggcgagg acgcagctcg aacacgaact cgcgctgatc
     2041 gcggaactcg cctacgaacc gtattttctt accgtgcacg acatcgtgcg tttcgcccgc
     2101 gaccgcggca ttctctgtca ggggcggggc tcggccgcga attccgccgt ctgctattgc
     2161 ctcggcatca ccgagctcga tccggcccgc tccaatcttc tgttcgagcg tttcgtctcg
     2221 aaggagcgcg ccgagccgcc cgacatcgac gtggacttcg aacacgagcg gcgcgaggaa
     2281 gtcattcaat acatctacgg aaaatacggg cgcgaacgtg cggcgctcgc cgcgaccctg
     2341 atcacctatc ggacgcgaag cgcggtgcgc gatctcggca aggcggatcg cgca
//
LOCUS       DASUJG010000410         2749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1098326, whole genome shotgun sequence.
ACCESSION   DASUJG010000410 DASUJG010000000
VERSION     DASUJG010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098326"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VFX38_08700"
     CDS             <1..185
                     /locus_tag="VFX38_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314966.1"
                     /translation="ANGGFGVDYPSAFEQASGSLGSGALWVTHFLALPSMWIGVGIGL
                     GLLVLATIARRYSATS"
     gene            200..1117
                     /locus_tag="VFX38_08705"
     CDS             200..1117
                     /locus_tag="VFX38_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314967.1"
                     /translation="MNRISALALGLLAAAFWTHSAVAADGSDTSQAKTLSRTVDAAGL
                     ASVRLDADVGSITVRAGKTNTVHVEATASPANHGHFIFDWTFGPPVNELPPDLRLVTA
                     RRGTELVICLASSTSGCKAVTEAAEEATAGSGHRRVTVVAPGGGFHVEYGGDSNTAWK
                     ADWTLVVPARLALELRSGVGSADISGVGGGLQAHIGVGKVDAVLPRGPVSVNVGVGDA
                     DITVASPDYGSVHLAAGVGDVSFEVNGNKNTTGYDHQFTAASQELDGPGGPAYTLKAG
                     VGHVKLALGEHAGPHSAEGAQNGADDDAP"
     gene            1101..1478
                     /locus_tag="VFX38_08710"
     CDS             1101..1478
                     /locus_tag="VFX38_08710"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX5314968.1"
                     /translation="MTTLHEPAMIRRLAFSVLGIACVALAFTGCVLVTAPGESPHGEW
                     AGTYAQQQAAGQDRDRDIALRVRDALAADPQLKTLGLRIFVDGAEVTLCGRYPDAATR
                     SRAIAIVSSVDGVAGVDPDCLDH"
     gene            complement(1513..2316)
                     /locus_tag="VFX38_08715"
     CDS             complement(1513..2316)
                     /locus_tag="VFX38_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314969.1"
                     /translation="MLRKPIRLLRRTERISAPARRRRRALLVGVAVLGAAAAAAPAAL
                     SAPPAPVREALAMLQEGRGRLYHAWRYEETITSSQGKERIAHNPSRPPGDRWRVLEVN
                     GKKPSAEQQKQFAARAKDVASTIAASLGTASGWLSQSDYRLVAKSATTLTYQLRPRPD
                     RASTDDSAKLLQHLAGRFVIARADHRPVSLSLDNFESFSPRFGVRIDSFRFWASFRRL
                     GGADGPVVITRTGTSTGGKIFWLKGFQDRTEVILGNFAPASATAAAAAG"
     gene            complement(2279..>2749)
                     /locus_tag="VFX38_08720"
     CDS             complement(2279..>2749)
                     /locus_tag="VFX38_08720"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEX5314970.1"
                     /translation="AADLKVGIVDPEAVLAQSDAGQAAQAKLKAEGEQHQKDAQQKQD
                     SLKKEQDALQKNASVETQTAQQQAQQKFQADVAAYQKWAQDTQQDFSQEQQKVLQPLQ
                     ATLYDVIQSYGKDNGYDLILDKSAAIYAAGGMDVTSAILKAFNAAEAHSSSSSH"
BASE COUNT          422 a          956 c          901 g          470 t
ORIGIN      
        1 acgcgaacgg cggattcggc gtggactatc cctcagcgtt cgagcaagcg tccggatcgc
       61 tcggttcggg agcgctctgg gtcacgcatt ttctcgccct gccctcgatg tggatcggcg
      121 tcggcatcgg cctcgggctt ctcgtcctcg ccacgatcgc ccgccgctat tcggccacct
      181 cctgatcccg gagactgtca tgaaccgcat atccgcactc gccctcgggc ttctcgccgc
      241 ggccttttgg acccattcgg ccgtcgccgc cgacggcagc gatacctccc aggcgaaaac
      301 cttgagccgc accgtcgacg ccgccggact cgcaagcgtc cggctcgacg cggatgtcgg
      361 ctcgatcacc gttcgcgcgg gcaaaacgaa taccgtccac gtggaagcga cggccagccc
      421 cgcgaaccac ggccacttca tcttcgattg gaccttcggt ccgccggtga acgaacttcc
      481 gcccgatctg cgactcgtga ccgcgcgccg cggcaccgaa ctggtgatct gcctcgcttc
      541 gagcacgagc ggttgcaagg ccgtcaccga ggcggcggaa gaggccaccg ccggctccgg
      601 gcaccgtcgc gtcaccgtcg tcgcgcccgg gggtggcttc catgtcgagt acggaggcga
      661 ttccaatacc gcctggaagg ccgactggac cctcgtcgtc cccgcgcgcc tggcgctgga
      721 gttgcgaagc ggagtcggca gcgccgacat cagcggcgtc ggcggcggcc tgcaggcgca
      781 catcggagtc ggcaaggtcg acgccgtact tccgcgcggg ccggtgagcg tcaacgtggg
      841 agtgggcgat gccgacatca ccgtcgcgag ccccgactac ggctcggtac acctcgcggc
      901 cggagtgggc gacgtgagtt tcgaagtgaa cggaaacaag aacacgaccg gctacgatca
      961 tcagttcacg gccgccagtc aggaacttga cggtcccggc ggtcccgcgt acacgctcaa
     1021 ggccggtgtc ggacacgtga aactggcact cggcgagcac gccggcccgc attccgccga
     1081 aggggcgcag aatggagcgg atgacgacgc tccatgaacc cgcgatgatc cgccggctcg
     1141 ccttctccgt gctgggcatc gcctgcgtcg cgctcgcgtt cacgggctgc gttctcgtca
     1201 ccgctcccgg cgagagtcct cacggcgagt gggccggtac ctacgcgcag cagcaggccg
     1261 ccggacagga tcgcgaccgc gacatcgcgc ttcgggtgcg cgacgcgctc gccgccgatc
     1321 cgcagttgaa aacgctcggg ctgcgcatct tcgtcgatgg cgcggaagtt acgctttgcg
     1381 gccgctaccc ggacgccgcc acccgcagtc gcgccatcgc gatcgtgagc agcgtcgatg
     1441 gtgtcgccgg agtcgatccc gactgcctgg atcactgatc gcgggagcgc ggccgcgctc
     1501 gcgcaggtcg gctcagccgg ccgccgcggc cgccgtagcg cttgccggcg cgaaattgcc
     1561 gaggatcacc tcggtccgat cctgaaatcc cttgagccag aaaatctttc ccccggtgga
     1621 cgtgccggtg cgcgtaatta ccaccgggcc gtcggccccg ccgaggcgac gaaaactcgc
     1681 ccagaaccgg aagctgtcga tccgcacgcc gaagcgcggc gaaaacgatt cgaagttatc
     1741 gaggctgaga ctcaccgggc gatgatcggc gcgggcaatg acgaagcgcc cggccagatg
     1801 ctgcagcagc ttcgcggagt cgtccgtgct cgcccggtcg ggccgcgggc gcagttggta
     1861 ggtaagcgtg gtcgccgact tcgcgaccag ccggtagtca ctttgcgaga gccagcccga
     1921 agcggttccc agcgacgccg cgatcgtgct cgccacatcc ttggcacgcg cggcgaattg
     1981 tttctgctgc tcggcgctcg gcttcttgcc gttgacctcg agcacgcgcc agcgatcccc
     2041 gggcgggcgc gaaggattgt gcgcgatccg ctccttgccc tgggagctgg tgatcgtctc
     2101 ctcgtaacgc caggcgtgat agagtcggcc gcgcccctcc tgcagcatcg cgagcgcctc
     2161 gcgcaccggc gccggcggcg cagagagtgc cgccggcgcc gcggcggccg cggcgccgag
     2221 aaccgcgact ccgacgagca gcgcccgtcg ccggcgtcgt gccggtgcgg aaattcgctc
     2281 agtgcgacga agaagacgaa tgggcttccg cagcattgaa agccttgagt atggcggagg
     2341 tcacatccat gccgccggcc gcatagatgg ctgcgctctt gtcgagaatg agatcgtaac
     2401 cgttgtcctt gccgtagctc tggataacgt cgtagagcgt ggcctgcaga ggctgaagaa
     2461 ctttctgctg ctcctgggaa aagtcctgct gggtgtcctg cgcccacttc tggtaggccg
     2521 ccacgtccgc ctgaaacttc tgctgcgcct gctgttgcgc ggtctgggtt tcgacgctgg
     2581 cattcttctg cagggcatcc tgctccttct tgaggctgtc ctgcttctgc tgggcatcct
     2641 tctgatgctg ctcgccctcc gccttgagct tcgcctgcgc tgcctgcccg gcatcgctct
     2701 gggcaagtac cgcctcggga tccacgatcc ccaccttgag gtcggcggc
//
LOCUS       DASUJG010000411         2340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_119678, whole genome shotgun sequence.
ACCESSION   DASUJG010000411 DASUJG010000000
VERSION     DASUJG010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119678"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VFX38_08725"
     CDS             <1..555
                     /locus_tag="VFX38_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HEX5314971.1"
                     /translation="FRLSMRRVVAAEEQGAPLDLTPAEEAGQAIARREEEFMYDGSKE
                     TGLEGLLGIAGAAKVRIGDWKNAGQVLTDMLTAVAALEKNGFLGPYALALPPKQYTLL
                     FRRYEGSDVLQLEHLKRLCERGVYKAEIARPVLVDPNVGHIVIGQDLRCGFSGTDGIH
                     YQLFANESVVLKVEEPGAVCVLGG"
     gene            599..1543
                     /locus_tag="VFX38_08730"
     CDS             599..1543
                     /locus_tag="VFX38_08730"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX5314972.1"
                     /translation="MDRPSPYRILALDGGGIRGYLSALLLARLERERPGLLGRFDLLA
                     GTSTGSIIALSLAAGFSPAEIAELYAHNVRRIFETTLARRLREANGLLGPRYGVQRLA
                     AALRTVFSDRRLGDLPRKVLVPAFDLDDENPDPARRRWKSRLFHNFPGDGGDAACLLR
                     KVALYSSVVPAVFASVDGYVDGGLFAANPALCAVALALEKCGDANGSHIPDLRLLSLG
                     TGLGPLHLGEETEQWGLAQWARPLLGLVLDAGVGMVDYQCRQLLRGNYHRLNPWIEGP
                     AILLDDLSALDRLHEAAETADLAPALAWLDATILGAAR"
     gene            1540..2091
                     /locus_tag="VFX38_08735"
     CDS             1540..2091
                     /locus_tag="VFX38_08735"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX5314973.1"
                     /translation="MNLYLRLLTLWLRRRARPAVSPLAPSRLRLRVWPNDLDLFGHMN
                     NGRYLTVMDLGRLDIVLGSGLLKVMRRRRWYAVAATVEIQFLRPLKLWRRYCLTTEIL
                     DWDEFWFVFEQRFDVADKPAARALVRAQFRHERDPVATAEVFAAAGWNNPRPPGGGKL
                     VESLRVATELTASASMPGSAAGR"
     gene            complement(2241..>2340)
                     /locus_tag="VFX38_08740"
     CDS             complement(2241..>2340)
                     /locus_tag="VFX38_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEX5314974.1"
                     /translation="RYRGLAKNANRLTTACALINLYRMRRQLLALP"
BASE COUNT          368 a          774 c          747 g          451 t
ORIGIN      
        1 ttccggctca gcatgcgccg cgtggtggcg gccgaggaac agggagcgcc gctcgatctc
       61 accccggcgg aggaggcggg gcaggcgatc gcgcgccgcg aggaggaatt catgtacgac
      121 ggcagcaagg aaaccgggct cgaagggctt ctcggcatcg cgggcgcggc caaggtgcgc
      181 atcggcgatt ggaagaacgc cggtcaagtt ctcaccgaca tgctcacggc cgtcgcggcg
      241 ctggagaaga acggctttct cggaccctat gcgctcgcgc tgccgccgaa gcagtacacg
      301 ctgctctttc gtcgctacga gggaagcgac gtgctgcaac tcgaacatct caagcgcctg
      361 tgcgagcggg gcgtgtacaa ggccgagatc gcgcgtccgg tgctggtgga cccgaacgtc
      421 gggcacatcg tcatcggaca ggatctgcgc tgcggcttct ccggcaccga cggcattcac
      481 taccagctct tcgccaacga aagcgttgtc ctcaaggtcg aggaaccggg cgcggtctgc
      541 gttctcggcg gctgatcgct cccgtggcga ctccctgagc gatcccgcag cggcgcggat
      601 ggaccgcccc tcgccctacc ggatccttgc gctcgacggc ggaggcattc gcggttatct
      661 gagcgcgctc ctgctcgcgc gcttggagcg cgagcgcccc gggcttctcg gacgcttcga
      721 tcttctcgcg ggcacctcga ccggatccat catcgcgctc agcctcgccg cgggattttc
      781 ccccgcggaa atcgcggaac tgtatgcgca caacgtccgc agaatcttcg agacgacact
      841 ggcgcggcgt ttgcgcgagg ccaacggtct cctcggaccc cgctacggcg tgcagcggct
      901 cgcggccgcg ttgcgcaccg tattttccga tcgtcggctc ggagatcttc cgcgcaaggt
      961 gctcgttccg gccttcgatc tcgatgacga aaaccccgac ccggcgcgaa ggcggtggaa
     1021 atcccggctc tttcacaatt ttcccgggga cggcggcgat gccgcatgct tgctccgcaa
     1081 agtcgcgctc tattccagcg tagttccggc cgtgtttgcc tcggtggacg gctacgtgga
     1141 cggcggtctc ttcgccgcga atcccgcgct ttgcgccgtc gccctcgccc tcgagaaatg
     1201 tggcgatgca aacggctcgc acatcccaga tctacggctc ctgtcgctcg gcaccgggct
     1261 cggtccgctg cacctcggag aagagaccga acagtgggga ctcgcacaat gggcgcggcc
     1321 gctgctgggc ctggtgctcg atgcgggtgt cggcatggtg gattaccaat gccgacaatt
     1381 gttgcgcggg aattatcatc gtctcaatcc ctggatcgaa ggcccggcaa ttctgctcga
     1441 tgacctgagc gcgctcgatc gcctgcacga agccgccgag actgccgatc tcgccccggc
     1501 actcgcctgg ctggatgcga cgatcctcgg ggctgcacgg tgaatctgta tctgcgcctg
     1561 ctcacgctgt ggctgcgccg ccgcgctcgc cctgctgtct cgccgctcgc cccctcgcgg
     1621 ttgcgcctgc gcgtgtggcc caatgatctc gatctcttcg gccacatgaa caacggccgc
     1681 tacctcaccg tgatggatct gggtcgcctg gacatcgtgc tcggaagcgg gcttctcaag
     1741 gtcatgcgcc gtcgccgctg gtatgcggtc gcggcgacgg tcgagattca gttccttcgt
     1801 ccgctgaaac tctggcggcg atactgcctc acgaccgaaa ttctcgactg ggacgaattc
     1861 tggttcgttt tcgagcaacg cttcgatgtc gcggacaagc ccgccgcgcg cgcgctcgtt
     1921 cgcgcgcaat ttcgccacga acgcgacccc gtcgcgacgg cggaagtttt cgcggcggcg
     1981 ggatggaaca atccacgccc gccgggcggc ggcaagcttg tcgagtcgct gcgcgtcgcg
     2041 accgaattga ccgcaagcgc ttccatgcct ggtagcgccg ccggtcgcta gggaagctct
     2101 gaactatatc ggcttacggt gttgacgttg cggattgaca tgccgagccc atttttcgtg
     2161 ataaattcga gttgacgggg gggcttttgc gcttttttac ccgccggccg ctcatttggc
     2221 ggccagcgga cgcatctctc ctacggaagt gccaacagtt gtcgtcgcat ccgatacaga
     2281 ttgatcaagg cgcaggcagt agtcagccga ttggcattct tcgccagccc tcggtagcgc
//
LOCUS       DASUJG010000412         3353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_487863, whole genome shotgun sequence.
ACCESSION   DASUJG010000412 DASUJG010000000
VERSION     DASUJG010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487863"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="VFX38_08745"
     CDS             <1..976
                     /locus_tag="VFX38_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314975.1"
                     /translation="AALAASMAVLQKKLRGQRAALEKFNSAHLGELPQQQQLNLAALD
                     HLYERLQGDQAAELDAMNRRADLLARSGGSGGGKLAALKQKLQALRLHYTDQYPDVVN
                     LRREIAALESSGAGDAAAPGASPSQGQLAQIDARLSDLRSDESAVQKQIAAARGKLER
                     LPLTNQRIEALSEGDSQTSGVYSALLTQYEEARVVMATAGKDGSPYVMLEPARAPRTS
                     EGPKRLRFAVMLLVVCAVLAFAAMLLAERRDTTFHTLEELREFTSVPILATIPVINRR
                     RDRLGRFLRVGALVAIVVCAVVILGGVGYVAGHGNQPVAAILAGGTNG"
     gene            969..1877
                     /locus_tag="VFX38_08750"
     CDS             969..1877
                     /locus_tag="VFX38_08750"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEX5314976.1"
                     /translation="MAKTARMAEQEYSGRSTPLVVPDGSLDGVPEPLRRNGPQTDLRC
                     VSLVAPESYESGRYQQLRSTIERKEPDGRAVVLAVTSPSAGDGKTLTAINLAGAFAHN
                     SQFRVLIVDVDMRRASETMGRYFGLPAEGQAGLTDVLHRPESGLHPTLQVRGLPNFSV
                     LLRGRRDAHAYEMLASERFGEFVERVRAYYHYVILDAPPILPVPDNSAISRWIDGFLL
                     VVTANRTPREALAEAMRSIGEDKLKGIVLNRCEPLAKRYYSYYGRYGGRRRGYHANAF
                     RAGDPQTLYESPAWPPQQGGVEIERR"
     gene            1935..3293
                     /locus_tag="VFX38_08755"
     CDS             1935..3293
                     /locus_tag="VFX38_08755"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX5314977.1"
                     /translation="MSGELRREWWQPAVAADRHVVAAGTPALGQASSVSFWALIAFTI
                     VLVLAPQDHLSFLKPLHLAMLSALAAGGSYVIDRYRGHCLRAPPSPEFLWLGAIVLWA
                     IATLPFSLWPGGSYQTLTGLFLKAVVIFWLLARVVDSPARLKTVAWLLSLAAVPLSLS
                     AVKAFVASGGAQNPDLSLGLSRINGYEGGLTSNPNDLALMINLILPLTLGLVFCTRRW
                     GLRFLLCGGAVLDAIAILATYSRGGFLMLATIVCSYLVVLCRRGRYFAVIAFLALGAA
                     TVPMLPSGYMQRLSTIVSIQNDQTNSAQDRWRDMKAAARYAFRHPVIGAGIGMSQLEL
                     NQVRGPEWVEVHNVYLKYAMELGWVGLALYLLLFGAALRSTRRACLAAARAGERELFF
                     LAQAIGVSLVGFTVAAMFYPDAYEFYFFYFAGLAVAARMIGLRAEAAIGANSRAREIA
                     IR"
BASE COUNT          534 a         1096 c         1077 g          646 t
ORIGIN      
        1 ggcggctctc gcggccagca tggcagtgct gcaaaagaag ctccgcggac agcgggcggc
       61 gctcgagaag ttcaacagcg ctcatctggg ggagttgccg cagcagcagc agctcaatct
      121 cgccgcgctc gatcatctct acgagcgcct gcagggagac caggcagcgg aactcgacgc
      181 catgaaccgg cgcgccgatc ttctcgcgcg ctccggcggt tcgggcggtg ggaagctcgc
      241 cgcgctgaaa cagaaactgc aggcgcttcg tctgcattac accgatcaat atcccgacgt
      301 ggtcaacctg cggcgcgaaa tcgccgctct ggaaagcagc ggagcgggtg acgccgcggc
      361 gccgggcgct tcgccctcgc agggacaact cgcgcagatc gatgcgcgcc tgagcgatct
      421 gcgatctgac gagtccgcgg tgcagaagca gatcgcagcg gcgcgcggaa aactcgagag
      481 gctgccgctc acgaatcagc gcatcgaggc gctgagcgag ggtgacagtc agacgagcgg
      541 cgtgtactcg gccctgctta cgcaatacga ggaagcgcgg gtggtcatgg ctaccgccgg
      601 caaggacgga tcgccctatg taatgctgga gccggcgcgc gcgccgcgca cttccgaggg
      661 gccgaagcgc ctgcgctttg cggtcatgct cctcgtcgtc tgcgcggtgc ttgccttcgc
      721 ggccatgttg ctcgccgagc ggcgcgatac gacgtttcac acgctcgagg aactgcggga
      781 attcacctcc gtgccgatcc tcgcgacgat tccggtcatc aaccggcgcc gcgatcgcct
      841 tgggcgcttt ctgcgggtgg gcgcgcttgt cgcgatcgtt gtctgcgcgg tggtgatcct
      901 cggcggagtc ggctatgtcg caggtcacgg caatcagccg gtcgccgcga tccttgccgg
      961 tggcaccaat ggctaagacc gcgcgcatgg ccgagcagga atattccggg cgcagtacgc
     1021 ccctcgtcgt tcccgacggg agtttggacg gggtgccgga gcccttgcgg cgcaacggac
     1081 cgcagacgga tctgcgctgc gtgagcctcg tcgctccgga atcctacgag agcggacgct
     1141 atcagcaact gcgctcgaca atcgaaagaa aggagccgga cgggcgcgcc gtggtcctgg
     1201 ctgtcaccag tccctcggcg ggcgatggaa aaacgctcac cgctatcaat ctcgcgggcg
     1261 ccttcgcgca caactcacag tttcgcgtac tgatcgtgga cgtcgacatg cgcagggcct
     1321 ccgagaccat gggccgatac ttcggtttgc cggcggaggg ccaggccgga ttgacggatg
     1381 ttctccatcg cccggagtcg ggactgcatc cgacgctgca agtgcgggga cttccgaact
     1441 tctccgtgct gctgcgcggg cgacgcgatg cccatgccta cgagatgctc gcttcggagc
     1501 gtttcggcga atttgtcgag cgcgtgcgcg cttactacca ttacgtgatc ctcgatgcgc
     1561 cgccgatcct ccccgttccc gacaacagcg ccatttcccg ctggatcgac ggatttctgc
     1621 tcgtggtgac cgcgaaccgg acgccgcggg aggcgctggc ggaggcgatg cgcagcatcg
     1681 gcgaggacaa gctgaagggg atcgtgttga atcgctgcga accactggcg aaacgctatt
     1741 acagttacta cggaagatac ggtggacggc ggcgtggcta ccatgcgaat gcctttcgtg
     1801 ccggcgatcc gcaaacgctc tacgaatctc cggcctggcc tccgcaacag ggcggcgtcg
     1861 agatcgaaag acgttgatcc ccgccaaatc gccacatccc gccccggtcc ccccgcaacc
     1921 gtgagcggcg agagatgagc ggagaacttc gccgcgaatg gtggcagccc gcagtggcag
     1981 ccgatcggca cgtcgtcgcc gctggcacac ccgccctcgg gcaggcgtct tccgtttcct
     2041 tctgggcatt gattgccttc accatcgtcc tcgttctcgc cccccaggat catctttcct
     2101 tcctcaagcc cctgcatctg gccatgcttt cggcgctcgc cgccggtggc agctacgtta
     2161 tcgatcgcta tcgtggtcat tgcctgcgcg ccccgccaag tcccgaattc ctttggctcg
     2221 gagcgatcgt gttgtgggcc atcgcgacgc taccgttttc gctctggccg ggggggagct
     2281 accagaccct gaccggcctc tttctgaagg cggtcgtgat cttctggctg cttgcccggg
     2341 tcgtggacag cccggcgcga ctgaagacgg tcgcctggct cctcagcctc gcggcagtgc
     2401 cgctgtcgct gtcggcggtg aaggccttcg tcgcttcggg aggcgcccag aatcccgatc
     2461 tttcgctggg cctctcgaga atcaacggct acgagggtgg gctcacctcg aatccgaacg
     2521 atcttgcgct gatgatcaac ctcattcttc cgctcacgct cgggctggtg ttctgcaccc
     2581 ggcgttgggg gttgcgcttc cttctgtgcg gcggcgccgt actcgatgcg atcgcgattc
     2641 tcgccaccta ttcacgcggc ggatttctca tgctcgcgac tatcgtctgc agctatctgg
     2701 tcgtgctgtg tcggcgcggt cggtacttcg ccgtaatcgc gttcctcgct ctcggcgccg
     2761 ccaccgtgcc aatgttgcct tcgggctaca tgcagcggct ctctacgatc gtctcgatcc
     2821 agaacgacca gaccaattcc gctcaggatc gctggcgcga catgaaagcc gccgcccgct
     2881 acgcgttccg tcatccggtg atcggcgccg gaatcggcat gagccaactc gagttgaatc
     2941 aggtgcgcgg tcccgaatgg gtcgaggtac acaacgttta cctgaaatat gccatggagc
     3001 tcggctgggt cggcctcgcg ctgtatctgc tcttgttcgg agctgccctg aggagtaccc
     3061 gtcgcgcctg ccttgccgcg gcgcgagccg gggaacggga gctgtttttt ctcgcgcagg
     3121 cgatcggagt gagcctcgtc ggtttcacgg tggcggcgat gttctacccc gacgcctacg
     3181 agttctattt cttctatttc gcgggtctcg cggtcgccgc gcggatgatc gggcttcgcg
     3241 ccgaagccgc gatcggcgca aattctcgcg cgcgcgagat cgctatccgc tgaagtcgaa
     3301 gtcgccgcgt tggcgccggc catgccgatg cagccatccc gaaagtgcca ccg
//
LOCUS       DASUJG010000413         6782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_608346, whole genome shotgun sequence.
ACCESSION   DASUJG010000413 DASUJG010000000
VERSION     DASUJG010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6782
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608346"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1514
                     /locus_tag="VFX38_08760"
     CDS             165..1514
                     /locus_tag="VFX38_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HEX5314978.1"
                     /translation="MTILAQVERNLERAEEVGRSLDERANEATASQQSAPEPQKWPES
                     MANEAFHGLAGDVVRTFEPHTEADPVALLMQFLAAFGNCIGRTAYYTVEGDRHFTNEY
                     AVLVGETAKARKGTSWGRIRALFALLEDSWARDRVHSGLSSGEGLIWSVHDPIIGRER
                     TGKGADIRYVAVEADPGVTDKRLLIVESEFANVLRVLAREGNTLSRVIRDGWDRGDLA
                     TMTKNSPARATGAHLSIVGHVTAEELRRYLDRTEAANGFANRFLYLCVRRSKVLPFGG
                     TLGNDELKPLAQRLAATIGAAKNPAGVGMNDEARAIWTRVYPELSEGQPGLLGAITGR
                     AEAHVVRFALIYALLDQAQQIEPAHLSAALAIWEYAEASARYIFGATLGDPVADEIVR
                     ALQGAPKTRTELRDLFGRHCSATQIGRALTLLEEQGRAQHQEIGTGGRPAEIWSLRQ"
     gene            1511..2110
                     /locus_tag="VFX38_08765"
     CDS             1511..2110
                     /locus_tag="VFX38_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314979.1"
                     /translation="MSYLDTARQVMIELHGGETATKATKATKAPLLSHMSLLSQGCAD
                     EKAELKALVCDETGLQVRCFDACRGLPILPILPMALFASLTDDDRAAQYSGEEGPDVL
                     RAYALAIADRLKRDDEAAAEWLAEREAIGAESGEPLRTTCPVFRFKLTWNPELWHYLL
                     APPGTDLAEARATLKRKHGDALLSTETFKPGACPQGGPA"
     gene            complement(2117..2638)
                     /locus_tag="VFX38_08770"
     CDS             complement(2117..2638)
                     /locus_tag="VFX38_08770"
                     /inference="COORDINATES: protein motif:HMM:NF027431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4823 domain-containing protein"
                     /protein_id="HEX5314980.1"
                     /translation="MQKFIVMIATVIMLGACSSTDIQNITGSPGATLDPHQGVYVTVP
                     SAPTEGDNPTVGQYIATAVASHFSKSGVTAWIADSPSSFTGNLTAARQRGAGYMVVPV
                     LTNWAHNATQWSSNPSTFGLRIGIVNVVTGQQIRVDSITSESSHISFLGTDPKELLND
                     ALDDYLELLYPEK"
     gene            complement(2872..3402)
                     /locus_tag="VFX38_08775"
     CDS             complement(2872..3402)
                     /locus_tag="VFX38_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314981.1"
                     /translation="MAVLQQHGGTYMAGREPEPVHSGERRKSPDRAILAESLALGHSL
                     REAAAAAGYTYDSARHVARTEAVRKMIDARTRELWSEGAPLAQAVLARIIADESAPAQ
                     TRGNLALAWLDRAGHKPVQQVQEIPAPRSRDEIIAALKAAAEAGSREAIRIYQAVAGT
                     GAQLEEVAAQDAQTTH"
     gene            complement(3631..3706)
                     /locus_tag="VFX38_08780"
     tRNA            complement(3631..3706)
                     /locus_tag="VFX38_08780"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3671..3673),aa:Lys,seq:ttt)
     gene            complement(3802..4542)
                     /gene="queC"
                     /locus_tag="VFX38_08785"
     CDS             complement(3802..4542)
                     /gene="queC"
                     /locus_tag="VFX38_08785"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEX5314982.1"
                     /translation="MSGIEVGRQPNMESDKAKGAVILLSGGLDSATTLAIARADGWLC
                     HALSLDYGQRHRAELDAASRLAVALGAAEQRVVRIGLDALGGSALTDAAIAVPETSDE
                     AIPATYVPARNTIFLAIALACAETVGATDLFIGVNSVDYSGYPDCRPEFIDAFARLAN
                     VATRAGVEGGRFHVQAPLLRMSKAEIIRRGTQLGVDYSLTVSCYQADERGRACARCDA
                     CHLRREGFRTAGMADPTRYQARASRALG"
     gene            4686..>6782
                     /locus_tag="VFX38_08790"
     CDS             4686..>6782
                     /locus_tag="VFX38_08790"
                     /inference="COORDINATES: protein motif:HMM:NF025670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HEX5314983.1"
                     /translation="MSIHISIVRVLGLATLAIVGILPAFAAPAAAGIPTVAPGAPMPA
                     GLRAALYRSLASDGVSARFIGGDGCATLGSSLRGCFDAEGAHFNDSRAAALTLRLLSW
                     GRGATLRGIEKVQPVLHRNGASFEHGGIRAWWRVLPIGFEQGFTIAQRPAGSGELTLE
                     LAAGGNPRLHNGELQWGPLHYGQLAVTDARGHSVPAALSAQDGRIRISVHDGRAVYPL
                     TVDPLLWQEQAVNASDGHALDQFGFAVAISGTTAIIGAPAATVQGRDFEGAAYVFTES
                     GGVWSASQKLTPSDNLPRDSFGWSVALNGTTAVVGATGLLGSGASDIGDGAKQGAAYV
                     FAESGGVWSETQRLTAHDGINYDEFGWSVAISGTNIFVGALNSGHSATATGAVYVFTL
                     NDGVWARGPKLLPDDSVPSDGFGDSIAATADTLLVGSPLAEVNGQSKQGAVYVFAQSD
                     GSWTQSQKFTADDGATDNFFGDAVAVSGSTALIGASYATVAGNNYQGAAYVFGEAGGI
                     WTQQQKLVAADGAANDNFGTAVALAGSRALVAAPLAAVADNDRQGAAYVFEKSGGNWS
                     QAQKFVASDGASAAYFGWSVALDQAPVALIGASYAAVGDHAGQGEAYFESGSGGCPQG
                     YSEYDGSLPAGGRAGTPVYSAPEGIENAILTAPDDFSLYARFQNGQGRKLIHVSGHEV
                     HRWGPAGQYEWLVQASP"
BASE COUNT         1239 a         2205 c         2166 g         1172 t
ORIGIN      
        1 catccgatgg acgccttgag gtgcgtcgcc catgaggcgc gagttgtcgc gatcacggcg
       61 agcgacattg ccgaggggcg caccgtctcg gcgcaagacg cgaaccgagt agcgacggcg
      121 gcaggccgga taggagccgc acttgacgcg gcagggggtg cgatatgacg atccttgcgc
      181 aagtcgaacg caaccttgaa cgcgccgaag aagtagggcg ctcacttgac gaacgcgcga
      241 atgaagcgac agcgtcgcaa cagagcgcac cggaaccgca gaagtggccg gaaagtatgg
      301 cgaatgaagc ctttcacggg ctcgcgggcg acgtggtgcg cacgtttgag ccgcataccg
      361 aggccgatcc cgtggcgctg ctgatgcaat tccttgcagc attcggcaac tgcatcgggc
      421 gtaccgcgta ttacaccgta gaaggcgacc gtcattttac gaacgagtat gcggtgctgg
      481 tgggcgaaac cgccaaggcc cgcaagggta caagttgggg acgtatccgg gcactgttcg
      541 ctctgctcga agacagctgg gcgcgcgacc gagtgcattc ggggctatcg tcgggtgaag
      601 ggttaatttg gtccgtgcac gatccgatca tcgggcgcga gcgcaccggg aaaggtgccg
      661 acatacgtta cgtcgcagtc gaagccgatc ccggcgttac cgacaagcgc ctcctcattg
      721 tcgaatcgga gtttgcgaac gtgctgcgcg tactggcgcg cgagggcaac accctgtccc
      781 gtgtgattcg agacggctgg gaccggggcg atctggcgac catgacaaag aactcgcccg
      841 cccgtgctac cggcgcgcat ctgtccattg tcgggcacgt cacggccgag gaactgcgcc
      901 gctatctgga tcgcaccgag gccgcgaacg gattcgcgaa ccgcttcctg tacctgtgcg
      961 tgcggcgctc gaaagtgctg ccctttggcg gaacgctcgg gaacgatgaa ctgaagccgc
     1021 tggcgcagag actcgccgcg accatcggcg ccgcgaagaa ccccgccggt gtcggtatga
     1081 acgatgaggc gcgcgcaatc tggacgcgcg tatatcccga actgtccgaa gggcaacctg
     1141 gactactggg agcgattacc ggccgcgccg aggcgcacgt cgttcgcttt gcgctgatat
     1201 acgcgctgct cgaccaggcg caacagatcg aaccggccca cctgagcgcc gcgctggcga
     1261 tttgggagta tgccgaggcg agcgcccgct acatattcgg ggcgaccctg ggcgaccccg
     1321 ttgccgatga gatcgtgcgc gctctgcaag gtgcccccaa gactcgaacc gaactccgag
     1381 acctgttcgg ccgccattgc tccgctactc aaatcggcag ggcactgaca ctgctcgaag
     1441 aacagggacg cgcccagcac caagaaatcg gcaccggcgg gcgcccggcc gaaatctgga
     1501 gcctgcgaca gtgagctacc tcgacaccgc ccggcaagtc atgatcgaat tgcacggggg
     1561 cgagactgcg acaaaagcga ccaaagcgac caaagccccc cttttgtcgc atatgtcgct
     1621 tttgtcgcaa ggctgcgcag atgagaaagc cgaactcaaa gcgctggtct gcgatgaaac
     1681 cggcctgcaa gtccgctgct tcgatgcctg ccgcggtctg cctattctgc ctattctgcc
     1741 gatggcgctg ttcgcgtccc tgaccgacga cgaccgggcc gcccagtatt caggcgagga
     1801 aggcccggac gtgctccgcg cctacgccct ggcaatcgcc gacaggctca agcgcgacga
     1861 tgaggcggcg gctgaatggt tggccgagcg cgaggcgatc ggggccgagt ccggcgagcc
     1921 gctacgcacc acctgcccgg tattccgctt caagctgacg tggaatcccg aactctggca
     1981 ctacctgctt gccccgccgg gcacggacct tgccgaggcg cgggcgacac tcaagcgcaa
     2041 gcacggcgac gcgctcctat cgaccgagac cttcaagccc ggcgcctgcc cgcaaggggg
     2101 tccggcatga gcgccattac ttctccggat acagtaattc caagtagtcg tcaagcgcat
     2161 cattaaggag ttccttcgga tctgtgccca aaaaggaaat gtggctgctt tcgctagtga
     2221 tgctgtccac ccgaatctgc tgcccggtga ccacgttgac gattccgatc ctgagtccaa
     2281 acgtgctcgg attgctggac cactgcgttg cattgtgtgc ccagttggtg agtaccggaa
     2341 caaccatata accggccccg cgctgccttg cggctgtcag gtttcccgta aatgatgacg
     2401 gactgtccgc aatccatgcg gtgacgccgc ttttagagaa atggctggct acggccgtcg
     2461 caatatactg ccctacggtc ggattatcgc cctcggttgg cgcggagggt acggttacat
     2521 acacgccttg atgagggtcc aaagtcgcac cgggggaacc cgtgatgttt tgtatatcgg
     2581 tactcgaaca tgcccccaac atgattaccg tcgcaatcat gacaatgaac ttctgcatgg
     2641 cgtcccccat ctccgttacc gcggcaatct tactaccatc gcgcggccgg agttgacgga
     2701 agggccgtga gcgggcggca ggccgcactg gcttccacgt aaacgccgcc aatccttgag
     2761 gcaccccgtc tcgccgcggc gttagtgcca cgggcaaggc gtgtgcattg acgcgataaa
     2821 cctctttcac gcctcctgtg taaggttgcc tcacggcccg gagtcggcct tttagtgcgt
     2881 agtctgtgca tcctgagcgg ctacttcttc gagctgcgcg cccgttccgg cgacggcctg
     2941 gtagatgcgt atggcctcac ggctgccggc ttcggccgcc gctttgagcg ccgcgatgat
     3001 ttcatcccgc gaccggggcg ccgggatttc ctgcacctgc tgcacaggtt tgtgccctgc
     3061 tcgatcaagc cacgcgaggg cgagattgcc gcgagtctgg gcgggcgcgg actcatcggc
     3121 aatgatgcgg gccagcacgg cttgggctag cggcgcacct tcactccaaa gttcgcgggt
     3181 ccgagcatct atcattttgc gcactgcttc cgtgcgggcg acgtggcgcg cgctatcgta
     3241 ggtatatccc gccgccgccg ctgcctcccg cagtgaatgc ccgagcgcca aggattccgc
     3301 aaggatggcg cgatccggcg atttacgccg ttccccgctg tgaaccggct ccggttccct
     3361 tcccgccata tacgtgcccc catgctgttg caaaaccgcc acaaatagtt taccccttcg
     3421 gtgagtaacc ggccgcaccc ccgccggtga attagcgaga gttggaagga aaatccgggc
     3481 gcgcgttttt gccccccggc ctgcacttgc gatgccgacc gccgacggct ccgaggaagg
     3541 ttcaaacccc gcgaaatcgg ttcaagtgct tacacagcgc ttacacgaaa aacgcttcac
     3601 gaacggttga cgtgtaagcc attgattgta tggtgggcgg tcagggattc gaaccctgga
     3661 ccaacggatt aaaagtccgc tgctctaccg gctgagctaa ccgccccgaa aaagcatcgc
     3721 cgagcctgcc ttcgctggcc gggcaggccc gcagctcatt gcaaaccgca aggatgggcc
     3781 ggcaggcccg gctcgacgat tctacccgag cgcccgcgac gcacgagcct gatagcgagt
     3841 cggatcggcc atccccgcgg tgcgaaagcc ctcacggcgc aggtggcagg catcgcaacg
     3901 ggcgcaggcg cgtccccgct catctgcctg ataacaggag acggtaagcg aatagtccac
     3961 gcccagttgc gttccgcgcc ggatgatttc ggccttgctc atgcgcaaca gcggcgcttg
     4021 cacatgaaac cgcccgcctt ccactcccgc ccgcgtcgcc acgttcgcga gccgcgcgaa
     4081 tgcatcgata aattcggggc gacagtcggg atatccggag taatccacgc tgtttacgcc
     4141 gatgaagaga tccgtggctc ccacggtttc ggcacaggcg agcgcgatcg cgagaaagat
     4201 cgtgttgcgc gcgggcacgt aggtcgccgg aatggcttcg tcggatgtct ccggcacggc
     4261 gatcgccgca tcggtcaggg ccgagccgcc caaggcgtcg aggccgatgc gaacgacgcg
     4321 ttgctcggcc gcgccgagcg cgacggcgag ccttgacgcc gcgtcaagct ccgcacgatg
     4381 gcgctgcccg tagtcaaggc tcagcgcatg acaaagccat ccgtccgcgc gcgcaatggc
     4441 aagcgtcgtt gccgaatcga gccctccgga gagaagaatt accgcgccct ttgccttgtc
     4501 cgattccatg ttcggctgcc gcccgacttc gattccgctc atgacgatac cttagcggcg
     4561 cacgggacga aggacaaggc gcatcgaagc gctggccggt tcaccgccga gccttgccag
     4621 tgtatattga gccgatcgca gcatgagcca tcgcgccggt ccgaccggca gagaggggaa
     4681 ttgccatgag catccacatt tccattgtcc gtgttctggg cctcgcgacg ctggccatcg
     4741 tcggcatcct gccggcattc gccgctccgg cggccgccgg gatcccaacg gttgcgccgg
     4801 gggccccgat gcccgcaggc ctgcgggccg ccctctaccg gagtctcgcg agcgacggcg
     4861 tttccgcccg attcatcggc ggcgacggct gcgcgacctt gggttcgtct ctgcggggat
     4921 gcttcgatgc cgagggggcg catttcaacg actctcgcgc ggcagctctt actctgcgcc
     4981 tcctttcgtg ggggcggggc gcaacgcttc gcggcattga gaaggtgcaa cccgtccttc
     5041 accgcaacgg cgcaagcttc gagcatggcg gcattcgcgc atggtggcga gtgctcccga
     5101 tcgggttcga gcagggcttc acgatcgcgc aacgtccggc gggaagtggc gaactcacgc
     5161 tggaacttgc agcaggcggc aatccgcgct tgcacaacgg cgaattgcaa tggggaccgc
     5221 tgcactacgg ccaactcgcg gtcaccgacg cccgcggcca ctcggttccg gcggcgctga
     5281 gcgcgcaaga cggccgcatc cgcatttcgg tacatgacgg aagggcggta tatccgctca
     5341 cggtggatcc tcttctctgg caggagcagg cggtcaatgc aagcgatggt cacgcactgg
     5401 accaattcgg cttcgcggtg gcgatttccg gcacgaccgc catcatcggc gcgcccgccg
     5461 ccacggttca gggccgcgac ttcgagggcg ccgcctacgt cttcaccgaa agcggcggcg
     5521 tctggagcgc aagtcaaaaa cttactccca gtgacaatct tccgcgcgac agtttcggct
     5581 ggtcggtcgc gctcaacggc acgaccgccg tcgtcggcgc cacgggtctt ctcggttccg
     5641 gcgccagcga catcggcgac ggcgccaaac agggagcggc ctacgtcttc gccgaaagcg
     5701 gcggcgtctg gagcgagacg cagaggctca ccgcccacga cggcatcaac tacgacgaat
     5761 tcggctggtc ggtcgcgatc agcggaacga acattttcgt cggtgcgctg aactccggcc
     5821 acagcgcgac cgcgaccggc gccgtgtatg tgttcacgct gaacgatggc gtgtgggcgc
     5881 gggggccgaa gcttctcccg gacgacagcg tcccgagcga cgggttcgga gattcgatcg
     5941 ccgccaccgc cgacacgctt ctcgtcggct cgccgctggc cgaagtgaac gggcagagca
     6001 aacagggagc cgtgtacgtt ttcgcacaat cggatggatc ctggacgcag agccagaagt
     6061 tcacggccga tgacggcgcg accgacaact tcttcggcga tgccgtagcc gtttcgggat
     6121 ccacggcgct gatcggcgcg agttacgcga ccgttgccgg caacaattac cagggggcgg
     6181 cctacgtctt cggcgaggcg ggcggcattt ggacccagca gcagaaactg gtcgccgccg
     6241 atggcgcggc caacgacaac ttcggcaccg ccgtcgcgct cgccggctcg cgcgccctcg
     6301 tcgcggcccc gctcgccgcg gtcgcggaca acgatcgcca gggcgcggcc tacgttttcg
     6361 agaagtcggg aggaaactgg tcgcaggcgc agaaattcgt cgcctccgac ggcgcctccg
     6421 ccgcctattt cggctggtcg gtcgcgctcg atcaagcgcc cgtcgccctc atcggagcga
     6481 gttacgccgc ggtcggcgac catgcgggac aaggggaggc ctacttcgag agcggttcgg
     6541 gcggctgccc gcagggttac agcgagtacg acggcagtct cccggccggc ggacgtgccg
     6601 gcacgccggt ttacagcgcc ccggagggaa tcgaaaacgc cattctcacc gcacccgacg
     6661 atttttcgct ctacgcgaga ttccagaacg gacagggccg aaagctcatc cacgtttcgg
     6721 gccacgaggt ccaccgctgg ggtccggcgg ggcaatacga gtggctggtg caggcttcgc
     6781 cg
//
LOCUS       DASUJG010000414         7041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1465032, whole genome shotgun sequence.
ACCESSION   DASUJG010000414 DASUJG010000000
VERSION     DASUJG010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7041
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1465032"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VFX38_08795"
     CDS             complement(<1..684)
                     /locus_tag="VFX38_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX5314984.1"
                     /translation="MADEHILFFRDIGVDDIPIVGGKSASLGEMYRALAGEGVRVPNG
                     FAITASAYRYLIGAAGLEGELRKSLAVVRPGDTRSLAEGGARARKLIADAPLPADLEH
                     AILGAYAELRKEYGSELSLAVRSSATAEDLPTASFAGQQESYLNVRGDAELLDACRQC
                     FASLFTDRAISYRSERGFDHFDVALAITVMKMVRSDLGASGVMFSLDTESGFRDVVMI
                     NSAWGLGENV"
     gene            complement(674..1549)
                     /locus_tag="VFX38_08800"
     CDS             complement(674..1549)
                     /locus_tag="VFX38_08800"
                     /inference="COORDINATES: protein motif:HMM:NF012647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEX5314985.1"
                     /translation="MDTAAALRYVRGRQTPAGGFSFYRSWGVEEPSPADSFYALFSLA
                     ILGGEPLEHDACANWLQARQDAAGGFSSLAAGWQVLESLDLLGMPPERSAAPWLITRA
                     GILFEAVEAGSAEAPGELLAELARFVDLWRSTGHLRLEPRQRMAIIASLERLRDARGA
                     FPRDAPNLTDSATALRLIRALHLLPHRSLLAFARSCEDPLYGFRPVPQGHSTRLEVVA
                     SGLDVCAAFGAEARYTDAIRETLAACQHDGGGFSPQPGALPTLRDTWLAMRILQRLAP
                     PSIDSNKTRGSFDGR"
     gene            complement(1709..3526)
                     /locus_tag="VFX38_08805"
     CDS             complement(1709..3526)
                     /locus_tag="VFX38_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HEX5314986.1"
                     /translation="MTTMVQALVLLAAAVIAVPLIRRAGFSSVLGYLVAGVAIGPWLL
                     GLVRGVDEILHFSELGVVMLLFIIGLELQPARLWALRRPIFGMGIVQLALTTIVIALF
                     GRLLGLPAPAALLAGFGLALSSTAFVLQILAERKQLGTTQGRAAFSILLFQDLAVIPV
                     LVLLHLFYEPGHGEGTWQSIAVAIAVVAALLLGGRYLLRPLFRMVASLGVPETFTAAA
                     LLIVLGAGLALQSVGLSMSLGAFLAGVLLADSAYRHELQASIEPFEGLLLGLFFVAVG
                     MSVNLGLVLAHPLRVVGLTLALLLVKGIILFVVGKAFRMPARSARALAFTLPQGGEFA
                     FVLFGAAAGYGLLSRDTADLLILIVAFSMALTPFLTMFNERVLARLAVPAPAPEFDRI
                     EEDEHPVIIAGFGRFGQVVARILRTRKIPFTALEISAEQVDFVRRYGSKIYYGDASKL
                     ELLRAAKADRAKIFVLAIDDVEASLRTAEMVRRHFPNLMIYARARNRRHVHLLMELGV
                     KYIVRETLLSSMDLSAKVLSGLGVAEREVARTLEIFRVHDDATLKRQLEVFRDESRLI
                     QTAQEAARELESLFEADKAAEEAHAAPSILVEAQKRAGK"
     gene            complement(3799..5376)
                     /locus_tag="VFX38_08810"
     CDS             complement(3799..5376)
                     /locus_tag="VFX38_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5314987.1"
                     /translation="MSGHNTTLLGLWQEVGRHGELSASIGNLAAILAQEIPLVALILF
                     ELDETQRSVLPLAEWRKRGGGEPHHHTRLRWDDGAYRALKQWCANDVVEEFSRSAPWP
                     IALAPLESFAVGSSALVAPLRQGEQAGGLVMIVAGGAKDFAAGTSNRLRSTLDPFSAA
                     VANELRLRELARLRAAAEADRTALLSRLGRDSLDEVIIGATTGLQGVMHRVELVAKSD
                     TAVLLLGETGAGKEVIARTIHDGSARHGGPFLRVNCGAIPPELMDSELFGHERGSFTG
                     AIATRRGWFERADGGTLLLDEVAELPPAAQVRLLRVLQEGRIHRVGGERELAVDVRII
                     AATHRNLAGRVQEGSFREDLWYRLAVFPIILPPLRERQQDIPDLVEHFVRRAAGRLGI
                     PVPPVTAEDIAQLQAYRWPGNIRELAAVLERAVILGEGRQLATTSAMGVVPIAPHRDQ
                     VFAGPAPQPGNARESLDSGTSFDSVMRQHLELVLRQTHGKVEGRGGASGILKLNANTL
                     RAKLRRLGIRPADFRTG"
     gene            5590..6642
                     /locus_tag="VFX38_08815"
     CDS             5590..6642
                     /locus_tag="VFX38_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5314988.1"
                     /translation="MLTLSLVAGNVAPVLAAPAAPRVSLLNVSYSSNTAFYTDFNSAF
                     RTYWKKKTGQEVDIHMSYGGSGSQARAVMEGLDADVVTLALASDIDAIASKTGLIDLD
                     WQTRLPHDSAPYTSTIVFLVRKGNPRHIKSWSDLVRPGVEVITPNPKTSGGARWNFLA
                     AWGAVIYRDLGGFKIIEDRGQAERVADTDRDARLFVSQLYRHVPVMDSSARVAAATFV
                     QRGIGDVLLAWENEALLAVAKLGRGKFEIVVPKVSILAQPAVAVVTQNVLRHGTGVVA
                     RAYLQYLYSPAGQALAARHDFRPIYPRYAGANDRARFPALKLFTVKELFGGWEQVGNK
                     FFADGGIFDQMYAPHD"
     gene            6685..>7041
                     /locus_tag="VFX38_08820"
     CDS             6685..>7041
                     /locus_tag="VFX38_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314989.1"
                     /translation="MMLALLPGRVKERAAREIARRERSASAPDTHGGRRANVWQPQIA
                     PAFSGIVKALLSWIAAFAGICLLGTTAARATVSVYSNDKLSVGLGGLLQLRFKDDHPI
                     GGNSEQEFLVQCLRLEF"
BASE COUNT         1388 a         2197 c         2236 g         1220 t
ORIGIN      
        1 gacgttttcg ccgagccccc aggcggagtt gatcattacc acgtcgcgga aacccgattc
       61 ggtgtcgagc gaaaacatga cgcctgaggc gccgaggtcc gagcgaacca tcttcatcac
      121 cgtgatcgcg agcgcgacat cgaaatgatc gaagccgcgc tcgctgcgat agctgatcgc
      181 ccgatccgtg aacagactcg cgaaacactg gcggcaggcg tccagcagtt ccgcgtcgcc
      241 gcgcacgttg agatagcttt cctgctggcc ggcgaaactc gccgtcggca ggtcttcggc
      301 ggtcgccgaa ctgcgcaccg cgaggctcag ctcggagccg tattccttgc gcaactcggc
      361 gtaggcgccg agaattgcgt gctcgagatc ggcgggcagg ggcgcatcgg caatcagctt
      421 gcgggcacgc gcgccgcctt cggcaagcga acgcgtgtcg ccggggcgga ccaccgcgag
      481 cgattttcgc agctcgcctt ccaagccggc cgccccgatg aggtagcgat aggccgaggc
      541 cgtgatcgcg aaaccgttcg gcacgcgcac gccctcgccg gcaagcgcac gatacatctc
      601 gccgagcgag gcgcttttgc cgccgacgat cggaatgtcg tccaccccga tatcgcgaaa
      661 aaacaggatg tgttcatcgg ccatcgaaac ttcctcgcgt cttgttcgag tcaattgagg
      721 gcggcgcgag tcgctgcaga atgcgcatcg cgagccaggt atcgcgcagc gtcggcaagg
      781 ctccgggctg cggtgaaaaa cctccgccgt cgtgctggca ggccgcgagc gtttcccgaa
      841 tcgcgtcggt gtagcgggcc tcggcgccga aggcggcgca aacgtcgagt cccgatgcca
      901 ctacttcgag gcgggtgctg tggccctgcg gaacgggccg gaatccgtag agcggatcct
      961 cgcaactgcg cgcaaatgcg agcagactgc gatgcgggag caggtggagc gcacggatca
     1021 gccgcagggc cgtcgccgag tccgtgaggt tcggcgcatc gcgcggaaag gcgccacgcg
     1081 catcgcgcag ccgctccagg ctcgcgatga tcgccatccg ctgccgcggt tcgagtcgca
     1141 aatgtccggt gctgcgccac agatcgacga agcgcgcgag ttcggcgagc agttcccccg
     1201 gcgcttccgc ggagccggcc tcgaccgcct cgaacagaat gcccgcgcgg gttatgagcc
     1261 acggcgctgc gcttcgttcc ggcggcatgc cgaggagatc gagcgattcg agcacctgcc
     1321 aacccgcggc gagactgctg aatcctcccg cggcatcctg gcgtgcctgc agccagttcg
     1381 cacaggcgtc gtgctcgagc ggttctccgc ccagaatggc gagggagaaa agggcgtaaa
     1441 aactgtcggc gggactgggt tcctcgacgc cccaactgcg gtaaaaggag aagccgccgg
     1501 ccggcgtctg gcgcccgcgc acgtagcgca gggcggcagc ggtatccacc tcgcagggtg
     1561 acgggttcgc tcgcggcgcg atgcttgcca atgacacgga ccgtcggggt atacccgcat
     1621 tgtaagcgat gcgcgccgcc gcacaggcgc cgccgcagtg cagaaaaccg tttaggttag
     1681 ctcgccacct tattgagttg gcaaaaggtt attttccggc tcgcttctgt gcttccacca
     1741 gaatcgaagg cgcagcgtgc gcttcctccg cggccttgtc cgcctcgaac agcgattcga
     1801 gttcacgggc ggcctcctgg gcggtctgga tgagccggct ttcgtcgcga aaaacttcca
     1861 actgccgctt gagggtcgca tcgtcgtgca cacggaaaat ttcgagcgtt cgcgcgactt
     1921 cgcgctccgc gacgccgagc ccgctcaata ccttggccga caaatccatg ctcgaaagca
     1981 gggtttcgcg aacaatgtac ttcacgccca gttccatcag aagatgcaca tggcggcgat
     2041 tgcgcgcgcg ggcgtagatc atgagattgg gaaagtggcg ccggaccatc tccgcggttc
     2101 gcagcgacgc ttccacgtcg tcgatggcca gcacgaaaat tttcgcacga tccgccttcg
     2161 ccgcacgcag gagttcgagc ttcgatgcgt caccatagta gatcttgctg ccgtagcggc
     2221 gcacgaagtc cacctgctcg gcgctgattt ccagcgccgt aaacgggatt tttcgcgttc
     2281 gcaaaatgcg ggcgacgacc tggccgaaac gcccgaaacc ggcgatgatc acggggtgtt
     2341 cgtcttcctc gatgcgatcg aactccgggg ccggcgcggg caccgccagg cgcgcgagaa
     2401 cgcgctcgtt gaacatcgtc aggaacggcg tcagcgccat ggaaaacgcg acgatcagaa
     2461 tcagcagatc cgccgtgtcc cgcgaaagga gcccgtagcc cgccgcggct ccaaacagca
     2521 cgaacgcaaa ctccccgccc tgaggcaggg tgaaggcgag ggcacgggca cttcgagccg
     2581 gcatgcggaa ggcctttccg acgacaaaga gaatgatgcc cttgacgagc agcagcgcga
     2641 gcgtcagtcc caccacgcga agcggatgcg cgagtacgag cccgagattc accgacatgc
     2701 cgacggcaac gaaaaacaat cccagcaaca ggccctcgaa gggttcgatg ctcgcctgca
     2761 gttcgtggcg gtaggcggaa tccgcgagaa gcacaccggc cagaaacgcc ccgagtgaca
     2821 tcgaaagccc cacggattgc agcgcaaggc cggcgccgag cacgatcagg agagccgcgg
     2881 ccgtgaaggt ttccggaacc ccgaggctcg ccaccatgcg aaagagcgga cgaagcagat
     2941 agcggccccc gagcaggagc gcggcgacaa cggcgatcgc gacggcgatg ctctgccacg
     3001 tcccttcgcc gtgtccgggc tcatagaaca gatgcagcag cacgaggacg ggaatcacgg
     3061 cgagatcctg gaacagaagg atggaaaacg ccgcccgccc ctgggtcgtg ccgagctgct
     3121 tgcgttcggc aaggatctgc agcacgaagg ccgtggacga taacgcgagg ccgaagccgg
     3181 cgagaagagc cgcgggcgca ggcagcccca gaagacggcc gaagagagcg atgacaatcg
     3241 tcgtgagtgc aagctgcacg atgcccatgc cgaagatggg ccgacgcagc gcccacaggc
     3301 gcgccggctg gagttcgagg ccgatgatga agagcagcat cacgacgccc aactcggaga
     3361 aatgcaggat ttcgtcgacc ccgcgcacga gaccgagcag ccacggtccg atcgcgacgc
     3421 cggcaacgag ataaccgaga accgagctga aaccggcccg gcggatcagc gggacggcga
     3481 tcaccgccgc ggcaagcagc acgagagcct gcaccatggt ggtcattgtt cgaggcgccc
     3541 gggaacagcg cggatacctt actcgaaatt ctgctggcgc cgatatgccg cccagtccga
     3601 cgcctttcga gttccacgag ccagaaggag agttcgaagc accctgtaag caattgagaa
     3661 ccccgtggtt atgtacggcc ataagtgccc gcacgggact cttgacgggt gcgctaacaa
     3721 cctagcgtac ttgacataat atatcttatg cgcacttgca gatacgcagc gaaactgacc
     3781 tctttcgcgc agaaactgtt agccggtccg aaagtctgcc ggccggattc cgagtctgcg
     3841 cagcttggca cgcagcgtgt ttgcgttgag tttcaggatt ccggacgcgc cgcctcgacc
     3901 ctcgactttc ccgtgagtct gacgcaggac aagctcaagg tgttgccgca tgacgctgtc
     3961 aaaacttgtt ccgctgtcga gtgactcgcg cgcgttcccg ggttgcggcg cgggtccggc
     4021 gaatacctga tcacggtgcg gagcgattgg caccactccc atggcgctcg tggtggcaag
     4081 ttgtcgccct tcaccgagga tgacggcgcg ctcgaggacc gcggcaagtt cgcgaatgtt
     4141 gccgggccaa cggtaggctt gcaattgcgc gatgtcttcg gccgtgaccg gtgggaccgg
     4201 aatgcccagc ctgccggcgg cgcggcgcac gaagtgttcg acgaggtccg gtatgtcctg
     4261 ttgccgttcg cgcaggggcg gaaggatgat cgggaatacg gcgagccggt accagaggtc
     4321 ctcgcggaag ctgccttcct gcactctgcc cgcaaggttg cgatgagtgg cggcgatgat
     4381 gcggacatcc acggcaagct cgcgttcgcc cccgacgcga tggatccgcc cttcctgcag
     4441 cacgcgcaac agccggacct gcgcggccgg gggcaattcc gccacttcgt ccaggagcag
     4501 cgtgccgccg tccgcccttt cgaaccagcc gcgccgggtc gcgatggcgc cggtgaaact
     4561 tccgcgctcg tggccaaaca gttccgaatc catcagctcg ggggggatcg cgccgcagtt
     4621 gacgcgcaga aacggtccgc cgtgacgtgc cgaaccgtcg tgaatggtgc gcgcaatgac
     4681 ttcttttccg gcacccgttt cgccgagcag cagaacggcc gtatccgatt ttgccaccag
     4741 ctcgacccga tgcatcacgc cctgcagacc cgtcgtcgcg ccgatgatta cctcgtcgag
     4801 ggaatcgcgc ccaagacggg acaacagggc cgtgcgatcg gcttcggcgg cggcccgcag
     4861 ccgcgccagt tcacgcagcc gcaattcatt ggccacagcc gccgaaaaag gatcgagcgt
     4921 ggatcgcaga cgattgctcg tcccggcggc aaaatctttt gcaccgccgg caacgatcat
     4981 caccaggccg ccggcctgtt ctccctgccg caacggtgcc acgagcgcgg aactgccgac
     5041 ggcaaacgat tcaagggggg ccagggctat gggccacggt gcagatcgcg agaattcctc
     5101 gacgacatcg ttcgcgcacc attgctttag tgcgcgatag gccccgtcgt cccagcgcag
     5161 gcgcgtgtgg tgatgcggct cccctcctcc ccgcttgcgc cattcggcga gcggaagcac
     5221 ggagcgctgg gtctcatcca gctcgaacag aataagggcg acgagcggaa tctcctgcgc
     5281 caaaatggcc gccaggtttc cgattgatgc tgacagttcc ccgtggcgac ccacttcctg
     5341 ccaaagccct aacaaggttg tattgtggcc gctcatgtca ttgcaatagg acgaatgcta
     5401 cgcaatataa cacgcactgg cattgtaata aaactgattg tttggggatg tgttgatgaa
     5461 acgccaagaa acgtacgata aaacagctag ttgagccctt gacggttgat tggcacggtt
     5521 cttgcgagga tacaggcagg ctcctgtgga catacctgcg tggacaaatt gatcttccct
     5581 ctcgtggcaa tgcttacgtt gagtcttgtc gcggggaatg tcgcgccggt actcgctgcc
     5641 cccgcggcgc ctcgggtttc cctgcttaac gtctcttact cgtccaacac ggcgttctac
     5701 accgacttca attccgcctt tcggacgtac tggaagaaga aaaccgggca ggaagtggac
     5761 atccacatgt cctacggcgg ttcaggttca caggcccgcg ccgtgatgga gggactggac
     5821 gccgacgtgg ttacactcgc cctcgcctcc gacatcgacg ccatcgccag caaaaccggg
     5881 ctgatagatt tggactggca gacccggctc ccgcacgaca gcgcgcctta tacgtccacc
     5941 atcgtgtttt tggtacgcaa gggcaatccg agacacatca aaagttggag cgatctcgtc
     6001 agaccgggcg tcgaggtgat cacaccgaat ccgaagactt ccggcggcgc acgttggaat
     6061 ttcctcgcgg cctggggcgc ggtgatatat cgcgatttgg gtggcttcaa gattattgaa
     6121 gacaggggtc aggcggaaag ggtcgcggac accgacagag acgcacgttt gttcgtcagc
     6181 caactatacc gtcatgttcc ggtcatggat tcaagcgcgc gagttgccgc agcaactttc
     6241 gtgcagcgcg ggataggcga tgtgcttctc gcctgggaaa acgaggctct gctcgcagtt
     6301 gccaaactcg gccggggtaa atttgaaatc gtggtgccga aggtcagtat tctcgcgcag
     6361 ccggcggttg ccgtagtgac tcaaaacgtc ttgcggcacg gtacgggagt cgtggccaga
     6421 gcctatcttc aatacctgta ctctcccgcc ggacaggcgc tggcggcacg ccacgatttc
     6481 cggccgatct atccgcggta tgccggagcc aacgaccgcg cgcggtttcc ggcgctgaag
     6541 ctctttaccg tcaaagagct cttcggcggc tgggaacagg tgggaaataa attcttcgca
     6601 gacggcggca tatttgacca aatgtatgca ccgcatgatt gaggacgtcc ggatgttacg
     6661 tcgcccgagt ctgcggggac atcggtgatg ctcgcgctgt tgcctggtcg ggtaaaggag
     6721 cgggctgctc gagaaattgc tcgccgggaa cggtctgcgt ccgccccgga tacacatgga
     6781 ggccgtcgtg cgaatgtgtg gcagccgcag attgcgccgg cgttctccgg aattgtgaag
     6841 gcgcttctct cgtggattgc ggcgtttgcg ggcatctgcc ttctcggaac gacagccgcc
     6901 cgcgccaccg tgtccgttta ttcgaatgac aagctgagcg tcgggctggg cggactcctg
     6961 caactccggt tcaaggacga tcatccgatt ggtggcaact ctgaacagga gttcctcgtg
     7021 caatgcctgc gcctcgaatt t
//
LOCUS       DASUJG010000415         2131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_732164, whole genome shotgun sequence.
ACCESSION   DASUJG010000415 DASUJG010000000
VERSION     DASUJG010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732164"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2002
                     /locus_tag="VFX38_08825"
     CDS             <1..2002
                     /locus_tag="VFX38_08825"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEX5314990.1"
                     /translation="KPVMEGKAVLFKRFADIDSIDLEVDTKDVDAFVNCVRYLGPAFG
                     GVNLEDIKAPECFVIEQQLRELMDIPVFHDDQHGTAIITTAGLINALHLTGRNLKDVK
                     MVVNGAGAASIACVELIKRMGLPHDQAILCDTKGVIYQGRTEGMNQWKSGHAVTTKAR
                     TLAEAMQGADVFIGLSVKGAVTQDMVKSMAAKPIIFAMANPDPEITPEEAKAVRPDAI
                     VATGRSDYPNQVNNVLGFPYIFRGALDVRARTINDQMKIAAAEALAALARQDVPDEVD
                     AAYSGRRLRFGPDYIIPVPFDPRLISCVPAAVAKAAMDSGVARRPIIDMEDYKASLAG
                     RLDPAAASMHLIFERLHLNPRRVVFAEGEEERVIRAALAYRAAGYGTPVLIGRTERIH
                     DTIRQAGLGEAAGLEIVNAANSPHTRTYHEMLYARLQRRGSLLRDVHRMVNQDRNVFG
                     ACMVEAGHADAMVTGTTRSFAVCLEDVLKVIEVREHQTLMAISVVVARGRTVLIADTS
                     VHELPTAEQLADFAAQSASLARQLGHEPRVAFLSFSNFGNPPREKAERIREAVEVMDS
                     RHADFEYEGEMQADVALDYGLMKELYPFTRLSGPANILIMPALHSANITAKLMKQLGE
                     TVIGPVLVGLAKPVQIVQMGATVNDIVTSAAFAALATMVHAR"
BASE COUNT          368 a          725 c          708 g          330 t
ORIGIN      
        1 caagccggtg atggaaggca aggcggtgct gttcaagcgc ttcgccgaca tcgattcgat
       61 cgacctcgag gtcgacacca aggacgtcga cgccttcgtc aattgcgtgc gttatctcgg
      121 ccccgccttc ggcggcgtca acctggagga catcaaggcg ccggaatgct tcgtcatcga
      181 gcagcagctg cgcgagctga tggacatccc ggtgttccat gacgaccagc atgggaccgc
      241 gatcatcacc acggccgggc tgatcaacgc gctccatctc acgggccgga acctcaagga
      301 cgtgaagatg gtggtgaacg gcgcgggcgc cgcctcgatc gcctgcgtgg agctcatcaa
      361 gcgcatgggc ctgccgcatg atcaggcgat cctgtgcgac accaagggcg tcatctacca
      421 gggccgcacc gagggcatga accagtggaa gtccggccat gcggtgacga ccaaggcgcg
      481 caccctggcc gaggcgatgc agggcgccga tgtcttcatc gggctttcgg tcaagggcgc
      541 ggtgacccag gacatggtga agagcatggc ggcgaagccg atcatcttcg ccatggccaa
      601 ccccgatccg gagatcacgc cggaggaagc gaaggcggtg cggcccgacg cgatcgtcgc
      661 caccgggcgc agcgactatc cgaaccaggt caacaacgtc ctcggcttcc cctacatctt
      721 ccgcggcgcg ctcgacgtgc gcgcgcgcac catcaacgac cagatgaaga tcgcggcggc
      781 cgaagccctc gccgctttgg cgagacaaga tgtcccggac gaggtcgacg ccgcctattc
      841 cgggcggcgc ctgcgcttcg ggccggacta catcatcccg gtgccgttcg atccgcgcct
      901 catctcctgc gtgccggcgg cggtcgccaa ggcggcgatg gacagcggcg tcgcgcgccg
      961 gccgatcatc gacatggagg actacaaggc ctcgctcgcc gggcgcctcg atcccgccgc
     1021 ggcttccatg catctcatct tcgagcggct gcatttgaat ccgcgccgcg tggtgttcgc
     1081 tgagggcgag gaggagcgcg tgatccgtgc ggccctggcg taccgcgcgg cgggctacgg
     1141 cacgccggtg ctgatcgggc gaaccgagcg catccacgac accatccggc aggcgggcct
     1201 cggcgaggcg gcggggctgg agatcgtgaa cgccgccaac tcgccgcaca cccgaaccta
     1261 tcacgagatg ttgtatgcca ggctgcagcg ccgcggctcg ctgctgcgcg acgtgcaccg
     1321 catggtgaac caggaccgca acgtgttcgg cgcctgcatg gtcgaggccg gccacgccga
     1381 cgcgatggtg accggcacca cccgcagctt cgcggtctgc ctcgaggacg tgctgaaggt
     1441 gatcgaggtg cgcgagcacc agaccctgat ggccatctcc gtggtggtgg cgcgcggccg
     1501 caccgtcctc atcgccgaca cctcggtgca tgagctgccg accgccgagc agctcgcgga
     1561 cttcgcggcg caatcggcct cgctggcgcg gcaattgggg cacgagccgc gggtcgcctt
     1621 cctctcgttc tcgaatttcg gcaacccgcc gcgcgaaaag gccgagcgca tccgcgaggc
     1681 ggtggaggtg atggacagcc gccatgccga cttcgaatac gagggcgaga tgcaggccga
     1741 cgtcgcgctc gattacgggc tgatgaagga gctctacccc ttcacgcgcc tgtcggggcc
     1801 ggccaatatc ctcatcatgc cggcgctgca ctccgccaac atcaccgcca agctgatgaa
     1861 gcagctcggc gagacggtga tcggccccgt gctggtcggc ttggcgaagc cagttcaaat
     1921 tgtccagatg ggcgccaccg tcaacgacat cgtcacctcc gccgccttcg ccgcgctcgc
     1981 caccatggtg catgcgcggt gaagttaatg atcacgcgtc gccgcgctat tcaggtgtca
     2041 agcgcggtcg tcctggcagg ctgcgggtcg accaaacgga acacctcgcc gtccggcacg
     2101 agcgcgccca aggcggaagg ctctcccttg g
//
LOCUS       DASUJG010000416         2495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1465764, whole genome shotgun sequence.
ACCESSION   DASUJG010000416 DASUJG010000000
VERSION     DASUJG010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1465764"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1266)
                     /locus_tag="VFX38_08830"
     CDS             complement(<1..1266)
                     /locus_tag="VFX38_08830"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012983752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX5314991.1"
                     /translation="MPSRRELANAIRALAMDAVQKADSGHPGMPMGMADIAEVLFNDF
                     LRHNPANPKWPGRDRFLLSNGHGCMLQYAALHLSGYDLPMEEIKRFRQLHSITPGHPE
                     YGHTPGVEVTTGPLGQGIGNAVGLALAEALLAAEFNRPGHDIVDHHTYVFCGDGCLME
                     GVSHEAGSLAGTLGLHKLIMIYDDNGISIDGEVKGWFHDDSAKRFQAYGWNTIGPIDG
                     LDAAAVKKALGEAQTETKRPTLILARTIIGFGAPDKQGTAATHGSALGEDEVAAARKT
                     LGWNFPAFEIPDEIYAGWDAHERGAKYEADWRGRFEAYEKAHPELAAELHRRLEGRLP
                     DDWKDAAQAYIEKVAAEGKDRATRKASGAALAAWQPVLPELVGGSADLTPSNDTWFPD
                     AKVIQPGKPAGNYLHWGVREFGMTAALNGM"
     gene            1427..1669
                     /locus_tag="VFX38_08835"
     CDS             1427..1669
                     /locus_tag="VFX38_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX5314992.1"
                     /translation="MESVNVTEFRKHLPSYLKRVAAGKAIRVTSRGRAIARLVPEEDP
                     AEAARKRLADLRGTMIVGNIDAPFENVDWTGDADNL"
     gene            1653..2054
                     /locus_tag="VFX38_08840"
     CDS             1653..2054
                     /locus_tag="VFX38_08840"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX5314993.1"
                     /translation="MRIICDTNVLIFWADRPGRLTPRVRRILDTKDANRRLAIADITL
                     WEIAMLFGKGRLNRSAGVTGSEYMGQIIDALRLELLPITPEIAELSQSTIFRHNDPED
                     RLIAATAIHHNAPLVTSDKRLRALKGLETIW"
     gene            2099..2443
                     /locus_tag="VFX38_08845"
     CDS             2099..2443
                     /locus_tag="VFX38_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5314994.1"
                     /translation="MMQDTSPETARYLRERYAAMSGAERFLIGARMFDTARRIVLASL
                     PADLSPAERRRALFRRLYPELDPNFLQARPTTAKCCGIAGVRHLPRKRTLSAAQPDRR
                     ISRLSRFRENRE"
BASE COUNT          437 a          793 c          797 g          468 t
ORIGIN      
        1 catgccgttg agcgcggccg tcatgccgaa ttcgcgcacg ccccagtgaa gataattccc
       61 cgcgggcttg ccgggttgga tcaccttcgc atcgggaaac caggtgtcgt ttgagggagt
      121 gagatccgcc gagccgccga cgagttcagg caacaccggc tgccaggcgg caagcgcggc
      181 accggatgcc ttgcgggtcg cgcgatcctt gccttcggcg gcgaccttct cgatgtaggc
      241 ctgcgccgcg tccttccagt cgtcgggaag ccgcccttcg agacggcgat gaagttcggc
      301 cgcgagttcc ggatgcgcct tttcgtaggc ttcgaaacgc ccgcgccagt cggcctcgta
      361 cttcgcgccg cgctcgtgcg catcccagcc ggcgtagatt tcgtccggaa tctcgaaggc
      421 gggaaagttc cagccgagcg tcttgcgcgc cgccgcgacc tcgtcttcgc cgagggcgct
      481 gccgtgagtc gcggccgtgc cctgcttgtc gggcgcaccg aagccgatga tggtgcgcgc
      541 gaggatgaga gtcgggcgct tcgtttccgt ttgcgcttcg ccgagcgcct ttttcaccgc
      601 cgcggcgtcg agtccgtcga tcggcccgat ggtgttccag ccgtaggcct ggaagcgctt
      661 ggcgctgtcg tcgtggaacc agcccttgac ctcgccgtcg atcgagatgc cgttgtcgtc
      721 gtagatcatg atcagcttgt ggagaccgag cgtgcccgcg agcgagcccg cctcgtgcga
      781 cacgccttcc atgagacagc cgtcgccgca gaagacatag gtgtgatgat cgacgatgtc
      841 gtggccgggg cgattgaact cggcggcgag gagtgcctcg gcgagcgcga ggccgacggc
      901 gttgccgatc ccctgaccga gcggaccggt cgtgacctcg acgcctggcg tgtgcccgta
      961 ttcgggatgc ccgggcgtga tcgagtgcaa ctggcggaag cgcttgatct cctccatcgg
     1021 caggtcataa cccgaaagat gcagcgccgc gtactgcagc atgcagccgt ggccgttcga
     1081 gaggagaaag cgatcgcgcc ccggccattt cgggttcgcc gggttgtggc gcaggaaatc
     1141 gttgaacagc acctcggcga tgtccgccat gcccattggc atgccgggat gcccggaatc
     1201 cgccttctgc acggcatcca tggccagcgc gcggatggca ttggcaagtt cgcggcgact
     1261 gggcatggag gactccggcg tgctgggttc tttattgtcg ccgactcgcg ccccagcggc
     1321 aaccgacgcg cgttcccgtc gcaggccgaa acacgcgccg catttccgac aagggtgggg
     1381 aggcgcaatc cggtaccatg tacataggtt ctgtacagtt cgaccgatgg aaagcgtaaa
     1441 cgtcaccgaa tttcgcaagc accttccctc ctatctcaaa cgcgtagcgg cgggaaaggc
     1501 gatccgcgtc accagtcgcg gccgtgcgat cgcgcgcttg gtcccggaag aagatcccgc
     1561 cgaagccgcg cgcaaacggc ttgccgacct gcgcggaacg atgatcgtgg gcaatatcga
     1621 cgcgcccttc gaaaacgtgg attggaccgg cgatgcggat aatctgtgac acgaatgtcc
     1681 tcatcttctg ggccgatcgc cctgggcgct tgacgcccag ggtcagacgg attctggaca
     1741 cgaaagatgc gaatcggcgc ctcgctattg ccgacatcac actttgggag attgcgatgt
     1801 tgttcgggaa ggggcgcttg aatcgttcgg caggagtcac cggatctgaa tacatgggtc
     1861 aaatcattga tgcgttgcgt ctggagttgc taccgatcac cccggagatt gccgagctgt
     1921 cgcaatcgac cattttccgg cataacgatc cggaagaccg ccttattgcg gcaactgcaa
     1981 tccatcacaa cgcaccgctt gtcaccagcg acaagcgact gcgcgcgctc aagggtctgg
     2041 aaacgatctg gtgaccgtcc gaaacggcag atcgcgaaat cgttgcgctg gtgtttcgat
     2101 gatgcaggac acgagccctg aaaccgcccg ctacctgcgc gagcgttacg ccgcgatgag
     2161 cggcgccgag cgtttcctga tcggcgcgcg catgttcgat accgcgcggc gcatcgtgct
     2221 cgcgtcgctg cccgcggact tgtcgcccgc cgagcggcgc cgtgccctct tccgccggct
     2281 ctatcccgaa ctcgatccga atttcttgca agcccgccct acgactgcaa aatgctgcgg
     2341 catcgcgggg gtacgccacc ttccccgtaa gcgcactctt tcggctgcgc aaccggacag
     2401 gcgaatttcc cggttgtcac gattccgcga gaaccgggaa taacactcat gcccgcgatc
     2461 agcagacgat gtaggccggg ataagcacag cgttc
//
LOCUS       DASUJG010000417         3310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1710895, whole genome shotgun sequence.
ACCESSION   DASUJG010000417 DASUJG010000000
VERSION     DASUJG010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1710895"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VFX38_08850"
     CDS             complement(<1..486)
                     /locus_tag="VFX38_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020559662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5314995.1"
                     /translation="MAQYVYTMNRVGKVVPPGRHILKDISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGVDSEIEGEARPQPGIRIGYLPQEPRLDPAKDVRGNVETGLGEIAAAQA
                     RLEEIYAAYAEPEADFDALATEQAKLENVVQAADAHNLEHRLEVAAEALRLPPWEADV
                     AM"
     gene            580..1833
                     /gene="glyA"
                     /locus_tag="VFX38_08855"
     CDS             580..1833
                     /gene="glyA"
                     /locus_tag="VFX38_08855"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011260263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX5314996.1"
                     /translation="MFAADLGIEDFDEELAAALANERRRQEDHLELIASENYVSPRVL
                     AAQGSVLTNKYAEGYPGKRYYGGCEYVDVAESLAIARAKELFDCDYANVQPHSGSQAN
                     QAVYFALLKPGDTILGMALAHGGHLTHGAKPNLSGRIFNAVQYGVNDEGLIDYDEVER
                     LAEEHRPKMVVAGFSAYSQVVDWARFRAIADQVGAFLFVDMAHPAGLIATGLYPSPLP
                     HAHVVTSTTHKTLRGPRGGLIVAKGAGEEIEKKLQSIVFPALQGGPLMHVIAAKAVAF
                     KEALQPEFRTYQQQVLVNAKALAEELAVRGYKIVSGGTEDHLFLVDLIARDITGKDAE
                     AALGAANITLNKNAVPNDPRSPFVTSGLRIGTPAATTRGLGEAEIRQVGEWIASILDD
                     LGHEQRIKAVKSEVLAMMQRFPVYG"
     gene            2110..2637
                     /gene="nrdR"
                     /locus_tag="VFX38_08860"
     CDS             2110..2637
                     /gene="nrdR"
                     /locus_tag="VFX38_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007042623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX5314997.1"
                     /translation="MHCPFCGHEDTRVADSRLAAGGRQIRRRRVCLKCRARFTTQETP
                     EFTLPRVVKRDGSRVPFDEEKLRVGVLKALEKRPVATETVDAMLANIVERLRLSGERE
                     MTSGAIGEMVMTALFDLDQVAYVRFASVYRSFQDVEAFRREIDQLEHQRGRPGRHRTP
                     DDGQLPLLDGKTAGK"
     gene            2661..>3310
                     /gene="ribD"
                     /locus_tag="VFX38_08865"
     CDS             2661..>3310
                     /gene="ribD"
                     /locus_tag="VFX38_08865"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX5314998.1"
                     /translation="MRRALRLAVRGLRSCAPNPAVGCVIVKDGRILGEGFHRRAGEAH
                     AEVLALAAAGADAHGATAYTTLEPCSHRGRTPPCAPALAAAGIARVVAAMEDPNPRVA
                     GGGFAALHAAGIEVETGIEAEKARRINRGFCSRFERGRPWVTLKLAASLDGRSALADG
                     TSRWITGEISRAAVQRERVRAGAVLTGVGTILADDPALDVRLPGAMRQPPRVVLDST"
BASE COUNT          561 a         1078 c         1120 g          551 t
ORIGIN      
        1 catcgcgaca tcggcttccc agggcggcag gcggagcgcc tcggcggcga cttcgagccg
       61 gtgttcgaga ttgtgcgcat ccgcggcctg gacgacgttc tcgagcttcg cctgctcggt
      121 ggcgagggca tcgaaatcgg cctcgggctc ggcgtaggcg gcatagattt cctcgaggcg
      181 cgcctgggcc gcggcgattt cgccgagccc ggtttcgaca ttgccgcgca cgtccttcgc
      241 cggatcgagc cgcggctcct gcggcagata gccgatgcgg atgccgggct gggggcgcgc
      301 ctcgccctcg atctcgctgt ccacgccggc catgatccgc agcagcgtgg atttgcccgc
      361 gccgttgagg ccgagtaccc cgatcttcgc gccgggaaag aaggacagcg agatgtcttt
      421 caggatgtgc cggccgggcg gaacgacctt gcccacccgg ttcatggtgt aaacgtattg
      481 cgccatcgtg gccggtggtt gcggcgggac tgcctattat aaaagcggcg cgggaagcgg
      541 gcaggctaca atttgcctcg tacccgccgg agcgcgcaga tgtttgcagc cgatctcggg
      601 atcgaggact tcgacgagga gctcgccgcg gcgctcgcga acgaaagacg gcgccaggag
      661 gaccacctcg agctgatcgc ctcggaaaat tacgtgagcc cgcgggtgct cgccgcgcag
      721 ggctcggtgc tcacgaacaa gtacgcggag ggctatcccg gcaaacgata ttacggcggc
      781 tgcgaatacg tggatgtggc ggaatcgctc gcgatcgcaa gggcgaagga actgttcgac
      841 tgcgactacg ccaacgtgca gccgcattcg ggttcgcagg cgaatcaggc agtgtatttc
      901 gcgctgctca agcccggcga cacgattctc ggcatggcgc tcgcccacgg cgggcatttg
      961 acgcacggcg cgaagccgaa tctttccggc aggattttca acgccgtgca gtacggcgtg
     1021 aacgacgagg gcctgatcga ttacgacgaa gtcgaacggc tggccgaaga gcaccgcccg
     1081 aagatggtcg tcgccggctt cagcgcctat tcgcaggtcg tggactgggc gcgctttcgc
     1141 gccattgcgg accaggtcgg cgccttcctg ttcgtggaca tggcgcatcc ggccgggctg
     1201 attgcgacgg gcttgtatcc gagtccgctg ccgcatgcgc acgtcgtcac ttcgaccacc
     1261 cacaagaccc tgcgcggccc gcgcggcggc ctgatcgtgg cgaaaggcgc gggcgaggag
     1321 atcgaaaaga aacttcagtc catcgttttt ccggcgcttc agggcggccc gctcatgcat
     1381 gtgatcgcgg ccaaggcggt ggccttcaag gaagccctgc aaccggagtt ccggacgtat
     1441 caacagcagg tgttggtgaa tgccaaggcg ctcgccgagg agctggccgt gcgcggctac
     1501 aagatcgttt ccggcggcac cgaggatcat ctttttctcg tggatctgat cgcgcgcgac
     1561 atcaccggca aggacgcgga ggcggcgctc ggtgcggcca acatcacgct caacaagaat
     1621 gcggtgccga acgatccgcg ctcgcccttc gtcaccagcg ggcttcgcat cggaacgccc
     1681 gcggcgacga cccgcggcct cggcgaggcc gaaatccgcc aggtgggaga atggatcgcg
     1741 agcatcctcg acgatctcgg gcacgagcaa cgcatcaagg cggtgaagag cgaggtgctc
     1801 gcgatgatgc agcgctttcc ggtttatggc tgagagattg gtactccgga ctccggactc
     1861 gggactcggg gttcagaatt ggaaatccgt ttcggacatg cggcaccgcg gatgcatcgg
     1921 aaaagacgtc cccccgcgtc tcgggcgcgg cccccttcgc cggcgaaggg ggcgaaaaca
     1981 tcgtcccttt gcctctgcga aaacatcgca tcccttcccc cctaggttca cgaacagcgc
     2041 attctttccc ccttcttcca gaagggggat tgagggggat gttccgagtc ccggctcccg
     2101 aggtttttga tgcactgccc gttttgcggc cacgaggaca cgcgggtcgc cgactcgcgg
     2161 ctcgcggcgg gaggacgcca gatccgccgc cgccgcgtct gcctcaaatg ccgggcgcgg
     2221 ttcacgacgc aggaaacgcc ggaattcacg ctgccgcggg tcgtcaagcg cgacggcagc
     2281 cgcgttccct tcgacgagga aaaacttcgc gtgggcgtac tcaaggcgct cgaaaaacgt
     2341 cccgtcgcga cggaaaccgt ggatgcgatg ctcgccaaca tcgtcgagcg cctgcgcctg
     2401 tccggcgagc gcgagatgac ctcgggcgcg atcggcgaaa tggtgatgac ggcgctcttc
     2461 gatctggacc aggtcgccta cgtgcgcttc gcctcggtgt atcgcagctt ccaggatgtc
     2521 gaggcctttc gtcgcgagat cgatcaactc gaacatcagc gcggccggcc cggtcgccac
     2581 cgcacgccgg acgacggcca gcttcccttg ctcgacggca aaacggccgg gaagtgagcg
     2641 gcgacgaaga ctgccgcctg atgcgccgcg cgctgcgact cgccgtacgc ggactgcgaa
     2701 gctgtgcgcc gaacccggcg gtcggttgcg tgatcgtgaa agacggacga atcctcggcg
     2761 agggctttca tcgtcgcgcc ggtgaggcgc atgccgaagt tcttgccctt gccgcggccg
     2821 gcgcggacgc tcacggcgct actgcctaca cgacgctcga gccatgcagt catcgggggc
     2881 gcacgccacc ctgcgccccg gcgctcgccg ccgccggcat cgcgcgggtc gtcgccgcga
     2941 tggaggatcc gaatccgcga gtcgcgggag gcggctttgc ggctctgcat gcggccggca
     3001 tcgaagtgga aaccggaatc gaagcagaga aggcgcgccg catcaatcgc ggcttctgca
     3061 gccgcttcga gcgcgggcgc ccctgggtca cgctcaaact ggcggcgagc ctcgacggca
     3121 gatcggctct cgccgacgga acgagccgct ggattaccgg cgaaatctca cgcgccgccg
     3181 tgcagcgcga acgtgtgcgc gccggcgcgg ttctcactgg cgtcgggacg atcctcgccg
     3241 acgatcccgc gctcgacgtg cgtcttcccg gagcgatgcg ccagccgccg cgcgtcgtcc
     3301 tcgattcgac
//
LOCUS       DASUJG010000418         2299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate SMAG_U1480
            k141_1466062, whole genome shotgun sequence.
ACCESSION   DASUJG010000418 DASUJG010000000
VERSION     DASUJG010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961629
            Sequence Read Archive: SRR25158533
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (soil metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:48:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,776
            CDSs (total)                      :: 1,739
            Genes (coding)                    :: 1,719
            CDSs (with protein)               :: 1,719
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 14 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1466062"
                     /isolate="SMAG_U1480"
                     /isolation_source="soil"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.64166667 N 123.6147222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..737
                     /gene="pgeF"
                     /locus_tag="VFX38_08870"
     CDS             24..737
                     /gene="pgeF"
                     /locus_tag="VFX38_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEX5314999.1"
                     /translation="MPAGVKAFVTTRAGGVSTASYASFNLGDHVGDDPAAVATNRRLL
                     RSALALPSEPCWLRQVHGRGIVRFEGRRSQEPLMADGAVSAEPGVVLAVLTADCLPVL
                     LAARDASEVAIVHAGWRGLAAGVIESAVGALGAEPEDLCAWLGPAIGAAHYEVGEEVR
                     AAFRGSAGAGEAFAPSPRRGHWRCDLAALARARLRHAGVPVVLGGGFCTFADRERFYS
                     YRRDGETGRMASLIWRAAA"
     gene            790..1566
                     /locus_tag="VFX38_08875"
     CDS             790..1566
                     /locus_tag="VFX38_08875"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEX5315000.1"
                     /translation="MSGNWAWVLALCGSAAIASAFGAVALAGLGERRLARMLPALVSF
                     STGVMLGAACLDLLPAVFAGAAAGRAANLGLALAGGILVFFALEKLIHWHHSHDIGPG
                     SAAQAENRATTVMVLTGTSLHNFLAGVLLAAAVLTDLKAALVLFAAILAHHIPQQLGD
                     FSVLLMTGYRRARALALTVASSAALFAGGVIAFVLLRHGRAALPFVVAVAAASLIYIA
                     LADLIPRLRGGHGGRQALAVIALVAAGIGVIYAVIRLLPG"
     gene            1745..>2299
                     /locus_tag="VFX38_08880"
     CDS             1745..>2299
                     /locus_tag="VFX38_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012983398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX5315001.1"
                     /translation="MRFDKLTSKFQMALADAQSLAVGRDHPFIEAAHLLLAMLEQDGG
                     SVRPLLAKAGVKVGVLENRLDAILDRLPQQKGTPGEVHVGNDLNRVLNITDKLAQDRK
                     DEFIAGELFVLACLEDRGAVGEALREAGAVKGAIERAIEEIRGGARVNDPEAEDKRQA
                     LEKYTQNLTARAERGKLDPVIGRDE"
BASE COUNT          316 a          763 c          831 g          389 t
ORIGIN      
        1 ccgggttttt gaccgcggat ggaatgccgg cgggcgtgaa ggcgttcgtg acgacgcgcg
       61 cgggcggtgt gagtacggcg agctacgcga gcttcaacct gggtgatcac gtcggcgacg
      121 atccggcggc cgtggcgacg aaccgccggc ttctgcgatc ggcccttgcc ttgccgtccg
      181 agccctgctg gttgcgccag gttcacggcc gcggcatcgt tcgtttcgaa ggcaggcggt
      241 cgcaggaacc gttaatggcc gatggcgccg tgagcgccga gcccggagtc gtcctcgccg
      301 tgctcacggc cgattgcctg ccggtgctgc ttgcggcccg ggacgcaagc gaggtcgcga
      361 tcgtgcatgc gggctggcgc ggcctcgccg ccggagtgat cgagtcggcc gtcggcgcgt
      421 tgggcgcgga gccggaagat ctctgcgcct ggctggggcc ggcgatcggc gctgcccatt
      481 acgaggttgg cgaggaggtg cgcgcggctt ttcgcggttc cgccggcgcc ggggaagcct
      541 tcgcgccctc gccgcgacgc gggcattggc gctgcgatct tgcggcactc gcccgggcgc
      601 ggctgcgcca tgccggcgtg ccggtggttc tcggaggagg cttctgtacc ttcgcggatc
      661 gcgagcggtt ttattcctac cgccgcgacg gcgagaccgg gcggatggcg agcctgatct
      721 ggcgcgccgc ggcttgacgg ctctggcagg attgaggcgg gtggctcgcg cggcaaaggg
      781 gcgggagcga tgagcggcaa ctgggcctgg gtgctggcgc tttgcgggag cgcggcgatc
      841 gcgagcgcct tcggcgcggt ggcgctcgcg gggctgggcg agcgccggct tgcgcggatg
      901 ctgccggcgc tcgtgagttt ctcgaccggg gtcatgctcg gcgcggcctg ccttgatctg
      961 ctgcccgcgg tcttcgccgg cgccgccgcg gggcgcgccg cgaatctcgg cctcgcgctc
     1021 gccggcggca ttctcgtttt cttcgcgctc gagaagctga ttcactggca tcattcgcac
     1081 gacatcggtc cgggcagcgc ggcgcaggcc gaaaatcggg caacgacggt gatggtgctc
     1141 accggcacca gcctgcacaa cttcctcgcc ggagtgcttc tcgcggcggc ggtgctgacc
     1201 gatctcaagg cggcgctggt actgttcgcg gccatcctcg cgcatcacat tccgcagcag
     1261 ctcggcgact tctcggtgct gctcatgacc ggctaccgcc gcgcccgcgc gcttgcgctc
     1321 accgtggctt cgagcgcggc gctcttcgcc ggcggcgtga tcgccttcgt gctgctgcgc
     1381 cacggccgcg cggccctgcc cttcgtcgtc gccgtcgcgg cggcgagcct catctacatc
     1441 gcgttggccg atctcatccc gcgtcttcgc ggcggccacg gcggacggca ggcgctggcg
     1501 gtgatcgcgc tggtggccgc gggcatcggc gtcatctacg ccgtcattcg cctccttccc
     1561 ggctgagaaa ggagagacga gaaagtcgaa aagtgagacg gtggcgacga cgcgtcgctg
     1621 acttccttgc aggcgcaccg cggccgactt gcagcgagca cggtgagcga cttgcgttat
     1681 cgcccgagtt gaaacgggat gaatccagcc ttatcttgaa accatctcgc cgggaatcca
     1741 gcccatgcgc ttcgacaaac ttaccagcaa gttccagatg gcgctcgccg atgcgcaatc
     1801 gcttgccgtc ggccgcgatc atcccttcat cgaggccgcg catctcctgc tcgccatgct
     1861 cgagcaggac ggcggcagcg tgcgaccgct gctcgcgaag gcgggtgtca aggtcggagt
     1921 gctggaaaat cgcctggatg ccatcctcga tcgcctgccg cagcagaagg gcacgcccgg
     1981 cgaggtccac gtcggcaacg atctcaatcg cgtgctcaac atcaccgaca agctcgcgca
     2041 ggatcgcaag gatgaattca tcgcgggcga actcttcgtg ctcgcctgtc tcgaagaccg
     2101 tggcgccgtc ggcgaggcgc tccgcgaggc gggcgcggtc aagggcgcga tcgaacgcgc
     2161 gatcgaggag atccgcggcg gcgcgcgggt gaacgatccc gaggccgagg acaagcgcca
     2221 ggcgctcgaa aaatacaccc agaatctcac ggctcgcgcc gagcgcggca agctcgatcc
     2281 ggtgatcggg cgcgacgag
//
